id sid tid token lemma pos cord-298078-uqrwq5qk 1 1 key key NN cord-298078-uqrwq5qk 1 2 : : : cord-298078-uqrwq5qk 2 1 cord-298078-uqrwq5qk cord-298078-uqrwq5qk NNP cord-298078-uqrwq5qk 2 2 authors author NNS cord-298078-uqrwq5qk 2 3 : : : cord-298078-uqrwq5qk 2 4 Kwak Kwak NNP cord-298078-uqrwq5qk 2 5 , , , cord-298078-uqrwq5qk 2 6 Hoyun Hoyun NNP cord-298078-uqrwq5qk 2 7 ; ; : cord-298078-uqrwq5qk 2 8 Park Park NNP cord-298078-uqrwq5qk 2 9 , , , cord-298078-uqrwq5qk 2 10 Min Min NNP cord-298078-uqrwq5qk 2 11 Woo Woo NNP cord-298078-uqrwq5qk 2 12 ; ; : cord-298078-uqrwq5qk 2 13 Jeong Jeong NNP cord-298078-uqrwq5qk 2 14 , , , cord-298078-uqrwq5qk 2 15 Sunjoo Sunjoo NNP cord-298078-uqrwq5qk 2 16 title title NN cord-298078-uqrwq5qk 2 17 : : : cord-298078-uqrwq5qk 3 1 Annexin Annexin NNP cord-298078-uqrwq5qk 3 2 A2 A2 NNP cord-298078-uqrwq5qk 3 3 Binds bind VBZ cord-298078-uqrwq5qk 3 4 RNA RNA NNP cord-298078-uqrwq5qk 3 5 and and CC cord-298078-uqrwq5qk 3 6 Reduces reduce VBZ cord-298078-uqrwq5qk 3 7 the the DT cord-298078-uqrwq5qk 3 8 Frameshifting Frameshifting NNP cord-298078-uqrwq5qk 3 9 Efficiency Efficiency NNP cord-298078-uqrwq5qk 3 10 of of IN cord-298078-uqrwq5qk 3 11 Infectious Infectious NNP cord-298078-uqrwq5qk 3 12 Bronchitis Bronchitis NNP cord-298078-uqrwq5qk 3 13 Virus Virus NNP cord-298078-uqrwq5qk 3 14 date date NN cord-298078-uqrwq5qk 3 15 : : : cord-298078-uqrwq5qk 3 16 2011 2011 CD cord-298078-uqrwq5qk 3 17 - - SYM cord-298078-uqrwq5qk 3 18 08 08 CD cord-298078-uqrwq5qk 3 19 - - SYM cord-298078-uqrwq5qk 3 20 30 30 CD cord-298078-uqrwq5qk 3 21 journal journal NN cord-298078-uqrwq5qk 3 22 : : : cord-298078-uqrwq5qk 4 1 PLoS PLoS NNP cord-298078-uqrwq5qk 4 2 One one CD cord-298078-uqrwq5qk 4 3 DOI DOI NNP cord-298078-uqrwq5qk 4 4 : : : cord-298078-uqrwq5qk 4 5 10.1371/ 10.1371/ CD cord-298078-uqrwq5qk 5 1 journal.pone.0024067 journal.pone.0024067 NNP cord-298078-uqrwq5qk 5 2 sha sha NNP cord-298078-uqrwq5qk 5 3 : : : cord-298078-uqrwq5qk 6 1 8bb9d8f7eeb8272924275f349e5c63a5bb006445 8bb9d8f7eeb8272924275f349e5c63a5bb006445 LS cord-298078-uqrwq5qk 6 2 doc_id doc_id CD cord-298078-uqrwq5qk 6 3 : : : cord-298078-uqrwq5qk 6 4 298078 298078 CD cord-298078-uqrwq5qk 6 5 cord_uid cord_uid NNS cord-298078-uqrwq5qk 6 6 : : : cord-298078-uqrwq5qk 7 1 uqrwq5qk uqrwq5qk NNP cord-298078-uqrwq5qk 7 2 Annexin Annexin NNP cord-298078-uqrwq5qk 7 3 A2 A2 NNP cord-298078-uqrwq5qk 7 4 ( ( -LRB- cord-298078-uqrwq5qk 7 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 7 6 ) ) -RRB- cord-298078-uqrwq5qk 7 7 is be VBZ cord-298078-uqrwq5qk 7 8 a a DT cord-298078-uqrwq5qk 7 9 protein protein NN cord-298078-uqrwq5qk 7 10 implicated implicate VBN cord-298078-uqrwq5qk 7 11 in in IN cord-298078-uqrwq5qk 7 12 diverse diverse JJ cord-298078-uqrwq5qk 7 13 cellular cellular JJ cord-298078-uqrwq5qk 7 14 functions function NNS cord-298078-uqrwq5qk 7 15 , , , cord-298078-uqrwq5qk 7 16 including include VBG cord-298078-uqrwq5qk 7 17 exocytosis exocytosis NN cord-298078-uqrwq5qk 7 18 , , , cord-298078-uqrwq5qk 7 19 DNA dna NN cord-298078-uqrwq5qk 7 20 synthesis synthesis NN cord-298078-uqrwq5qk 7 21 and and CC cord-298078-uqrwq5qk 7 22 cell cell NN cord-298078-uqrwq5qk 7 23 proliferation proliferation NN cord-298078-uqrwq5qk 7 24 . . . cord-298078-uqrwq5qk 8 1 It -PRON- PRP cord-298078-uqrwq5qk 8 2 was be VBD cord-298078-uqrwq5qk 8 3 recently recently RB cord-298078-uqrwq5qk 8 4 proposed propose VBN cord-298078-uqrwq5qk 8 5 to to TO cord-298078-uqrwq5qk 8 6 be be VB cord-298078-uqrwq5qk 8 7 involved involve VBN cord-298078-uqrwq5qk 8 8 in in IN cord-298078-uqrwq5qk 8 9 RNA RNA NNP cord-298078-uqrwq5qk 8 10 metabolism metabolism NN cord-298078-uqrwq5qk 8 11 because because IN cord-298078-uqrwq5qk 8 12 it -PRON- PRP cord-298078-uqrwq5qk 8 13 was be VBD cord-298078-uqrwq5qk 8 14 shown show VBN cord-298078-uqrwq5qk 8 15 to to TO cord-298078-uqrwq5qk 8 16 associate associate VB cord-298078-uqrwq5qk 8 17 with with IN cord-298078-uqrwq5qk 8 18 some some DT cord-298078-uqrwq5qk 8 19 cellular cellular JJ cord-298078-uqrwq5qk 8 20 mRNA mrna NN cord-298078-uqrwq5qk 8 21 . . . cord-298078-uqrwq5qk 9 1 Here here RB cord-298078-uqrwq5qk 9 2 , , , cord-298078-uqrwq5qk 9 3 we -PRON- PRP cord-298078-uqrwq5qk 9 4 identified identify VBD cord-298078-uqrwq5qk 9 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 9 6 as as IN cord-298078-uqrwq5qk 9 7 a a DT cord-298078-uqrwq5qk 9 8 RNA RNA NNP cord-298078-uqrwq5qk 9 9 binding bind VBG cord-298078-uqrwq5qk 9 10 protein protein NN cord-298078-uqrwq5qk 9 11 ( ( -LRB- cord-298078-uqrwq5qk 9 12 RBP RBP NNP cord-298078-uqrwq5qk 9 13 ) ) -RRB- cord-298078-uqrwq5qk 9 14 that that WDT cord-298078-uqrwq5qk 9 15 binds bind VBZ cord-298078-uqrwq5qk 9 16 IBV IBV NNP cord-298078-uqrwq5qk 9 17 ( ( -LRB- cord-298078-uqrwq5qk 9 18 Infectious Infectious NNP cord-298078-uqrwq5qk 9 19 Bronchitis Bronchitis NNP cord-298078-uqrwq5qk 9 20 Virus Virus NNP cord-298078-uqrwq5qk 9 21 ) ) -RRB- cord-298078-uqrwq5qk 9 22 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 9 23 RNA RNA NNP cord-298078-uqrwq5qk 9 24 . . . cord-298078-uqrwq5qk 10 1 We -PRON- PRP cord-298078-uqrwq5qk 10 2 first first RB cord-298078-uqrwq5qk 10 3 confirmed confirm VBD cord-298078-uqrwq5qk 10 4 the the DT cord-298078-uqrwq5qk 10 5 binding binding NN cord-298078-uqrwq5qk 10 6 of of IN cord-298078-uqrwq5qk 10 7 ANXA2 anxa2 NN cord-298078-uqrwq5qk 10 8 to to IN cord-298078-uqrwq5qk 10 9 IBV IBV NNP cord-298078-uqrwq5qk 10 10 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 10 11 RNA RNA NNP cord-298078-uqrwq5qk 10 12 by by IN cord-298078-uqrwq5qk 10 13 ultraviolet ultraviolet NN cord-298078-uqrwq5qk 10 14 crosslinking crosslinking NN cord-298078-uqrwq5qk 10 15 and and CC cord-298078-uqrwq5qk 10 16 showed show VBD cord-298078-uqrwq5qk 10 17 its -PRON- PRP$ cord-298078-uqrwq5qk 10 18 binding binding NN cord-298078-uqrwq5qk 10 19 to to IN cord-298078-uqrwq5qk 10 20 RNA RNA NNP cord-298078-uqrwq5qk 10 21 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 10 22 with with IN cord-298078-uqrwq5qk 10 23 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 10 24 protein protein NN cord-298078-uqrwq5qk 10 25 in in IN cord-298078-uqrwq5qk 10 26 vitro vitro FW cord-298078-uqrwq5qk 10 27 and and CC cord-298078-uqrwq5qk 10 28 in in IN cord-298078-uqrwq5qk 10 29 the the DT cord-298078-uqrwq5qk 10 30 cells cell NNS cord-298078-uqrwq5qk 10 31 . . . cord-298078-uqrwq5qk 11 1 Since since IN cord-298078-uqrwq5qk 11 2 the the DT cord-298078-uqrwq5qk 11 3 RNA RNA NNP cord-298078-uqrwq5qk 11 4 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 11 5 located locate VBN cord-298078-uqrwq5qk 11 6 in in IN cord-298078-uqrwq5qk 11 7 the the DT cord-298078-uqrwq5qk 11 8 frameshifting frameshifte VBG cord-298078-uqrwq5qk 11 9 region region NN cord-298078-uqrwq5qk 11 10 of of IN cord-298078-uqrwq5qk 11 11 IBV IBV NNP cord-298078-uqrwq5qk 11 12 was be VBD cord-298078-uqrwq5qk 11 13 used use VBN cord-298078-uqrwq5qk 11 14 as as IN cord-298078-uqrwq5qk 11 15 bait bait NN cord-298078-uqrwq5qk 11 16 for for IN cord-298078-uqrwq5qk 11 17 cellular cellular JJ cord-298078-uqrwq5qk 11 18 RBPs RBPs NNP cord-298078-uqrwq5qk 11 19 , , , cord-298078-uqrwq5qk 11 20 we -PRON- PRP cord-298078-uqrwq5qk 11 21 tested test VBD cord-298078-uqrwq5qk 11 22 whether whether IN cord-298078-uqrwq5qk 11 23 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 11 24 could could MD cord-298078-uqrwq5qk 11 25 regulate regulate VB cord-298078-uqrwq5qk 11 26 the the DT cord-298078-uqrwq5qk 11 27 frameshfting frameshfting NN cord-298078-uqrwq5qk 11 28 of of IN cord-298078-uqrwq5qk 11 29 IBV IBV NNP cord-298078-uqrwq5qk 11 30 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 11 31 RNA RNA NNP cord-298078-uqrwq5qk 11 32 by by IN cord-298078-uqrwq5qk 11 33 dual dual JJ cord-298078-uqrwq5qk 11 34 luciferase luciferase NN cord-298078-uqrwq5qk 11 35 assay assay NN cord-298078-uqrwq5qk 11 36 . . . cord-298078-uqrwq5qk 12 1 Overexpression overexpression NN cord-298078-uqrwq5qk 12 2 of of IN cord-298078-uqrwq5qk 12 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 12 4 significantly significantly RB cord-298078-uqrwq5qk 12 5 reduced reduce VBD cord-298078-uqrwq5qk 12 6 the the DT cord-298078-uqrwq5qk 12 7 frameshifting frameshifte VBG cord-298078-uqrwq5qk 12 8 efficiency efficiency NN cord-298078-uqrwq5qk 12 9 from from IN cord-298078-uqrwq5qk 12 10 IBV IBV NNP cord-298078-uqrwq5qk 12 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 12 12 RNA RNA NNP cord-298078-uqrwq5qk 12 13 and and CC cord-298078-uqrwq5qk 12 14 knockdown knockdown NN cord-298078-uqrwq5qk 12 15 of of IN cord-298078-uqrwq5qk 12 16 the the DT cord-298078-uqrwq5qk 12 17 protein protein NN cord-298078-uqrwq5qk 12 18 strikingly strikingly RB cord-298078-uqrwq5qk 12 19 increased increase VBD cord-298078-uqrwq5qk 12 20 the the DT cord-298078-uqrwq5qk 12 21 frameshifting frameshifte VBG cord-298078-uqrwq5qk 12 22 efficiency efficiency NN cord-298078-uqrwq5qk 12 23 . . . cord-298078-uqrwq5qk 13 1 The the DT cord-298078-uqrwq5qk 13 2 results result NNS cord-298078-uqrwq5qk 13 3 suggest suggest VBP cord-298078-uqrwq5qk 13 4 that that IN cord-298078-uqrwq5qk 13 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 13 6 is be VBZ cord-298078-uqrwq5qk 13 7 a a DT cord-298078-uqrwq5qk 13 8 cellular cellular JJ cord-298078-uqrwq5qk 13 9 RBP rbp NN cord-298078-uqrwq5qk 13 10 that that WDT cord-298078-uqrwq5qk 13 11 can can MD cord-298078-uqrwq5qk 13 12 modulate modulate VB cord-298078-uqrwq5qk 13 13 the the DT cord-298078-uqrwq5qk 13 14 frameshifting frameshifte VBG cord-298078-uqrwq5qk 13 15 efficiency efficiency NN cord-298078-uqrwq5qk 13 16 of of IN cord-298078-uqrwq5qk 13 17 viral viral JJ cord-298078-uqrwq5qk 13 18 RNA RNA NNP cord-298078-uqrwq5qk 13 19 , , , cord-298078-uqrwq5qk 13 20 enabling enable VBG cord-298078-uqrwq5qk 13 21 it -PRON- PRP cord-298078-uqrwq5qk 13 22 to to TO cord-298078-uqrwq5qk 13 23 act act VB cord-298078-uqrwq5qk 13 24 as as IN cord-298078-uqrwq5qk 13 25 an an DT cord-298078-uqrwq5qk 13 26 anti anti JJ cord-298078-uqrwq5qk 13 27 - - JJ cord-298078-uqrwq5qk 13 28 viral viral JJ cord-298078-uqrwq5qk 13 29 cellular cellular JJ cord-298078-uqrwq5qk 13 30 protein protein NN cord-298078-uqrwq5qk 13 31 , , , cord-298078-uqrwq5qk 13 32 and and CC cord-298078-uqrwq5qk 13 33 hinting hint VBG cord-298078-uqrwq5qk 13 34 at at IN cord-298078-uqrwq5qk 13 35 roles role NNS cord-298078-uqrwq5qk 13 36 in in IN cord-298078-uqrwq5qk 13 37 RNA RNA NNP cord-298078-uqrwq5qk 13 38 metabolism metabolism NN cord-298078-uqrwq5qk 13 39 for for IN cord-298078-uqrwq5qk 13 40 other other JJ cord-298078-uqrwq5qk 13 41 cellular cellular NN cord-298078-uqrwq5qk 13 42 mRNAs mrna NNS cord-298078-uqrwq5qk 13 43 . . . cord-298078-uqrwq5qk 14 1 Ribosomal ribosomal JJ cord-298078-uqrwq5qk 14 2 frameshifing frameshifing NN cord-298078-uqrwq5qk 14 3 is be VBZ cord-298078-uqrwq5qk 14 4 a a DT cord-298078-uqrwq5qk 14 5 recoding recoding JJ cord-298078-uqrwq5qk 14 6 process process NN cord-298078-uqrwq5qk 14 7 of of IN cord-298078-uqrwq5qk 14 8 translation translation NN cord-298078-uqrwq5qk 14 9 where where WRB cord-298078-uqrwq5qk 14 10 a a DT cord-298078-uqrwq5qk 14 11 specific specific JJ cord-298078-uqrwq5qk 14 12 messenger messenger NN cord-298078-uqrwq5qk 14 13 RNA RNA NNP cord-298078-uqrwq5qk 14 14 ( ( -LRB- cord-298078-uqrwq5qk 14 15 mRNA)-mediated mrna)-mediated JJ cord-298078-uqrwq5qk 14 16 signal signal NN cord-298078-uqrwq5qk 14 17 directs direct VBZ cord-298078-uqrwq5qk 14 18 a a DT cord-298078-uqrwq5qk 14 19 ribosome ribosome NN cord-298078-uqrwq5qk 14 20 to to TO cord-298078-uqrwq5qk 14 21 shift shift VB cord-298078-uqrwq5qk 14 22 its -PRON- PRP$ cord-298078-uqrwq5qk 14 23 reading reading NN cord-298078-uqrwq5qk 14 24 frame frame NN cord-298078-uqrwq5qk 14 25 and and CC cord-298078-uqrwq5qk 14 26 to to TO cord-298078-uqrwq5qk 14 27 continue continue VB cord-298078-uqrwq5qk 14 28 in in IN cord-298078-uqrwq5qk 14 29 the the DT cord-298078-uqrwq5qk 14 30 new new JJ cord-298078-uqrwq5qk 14 31 frame frame NN cord-298078-uqrwq5qk 14 32 . . . cord-298078-uqrwq5qk 15 1 Programmed-1 Programmed-1 NNP cord-298078-uqrwq5qk 16 1 ribosomal ribosomal JJ cord-298078-uqrwq5qk 16 2 frameshifting frameshifte VBG cord-298078-uqrwq5qk 16 3 is be VBZ cord-298078-uqrwq5qk 16 4 the the DT cord-298078-uqrwq5qk 16 5 most most RBS cord-298078-uqrwq5qk 16 6 widely widely RB cord-298078-uqrwq5qk 16 7 used use VBN cord-298078-uqrwq5qk 16 8 translational translational JJ cord-298078-uqrwq5qk 16 9 recoding recoding NN cord-298078-uqrwq5qk 16 10 mechanism mechanism NN cord-298078-uqrwq5qk 16 11 of of IN cord-298078-uqrwq5qk 16 12 many many JJ cord-298078-uqrwq5qk 16 13 viruses virus NNS cord-298078-uqrwq5qk 16 14 [ [ -LRB- cord-298078-uqrwq5qk 16 15 1 1 CD cord-298078-uqrwq5qk 16 16 ] ] -RRB- cord-298078-uqrwq5qk 16 17 . . . cord-298078-uqrwq5qk 17 1 Many many JJ cord-298078-uqrwq5qk 17 2 viruses virus NNS cord-298078-uqrwq5qk 17 3 employ employ VBP cord-298078-uqrwq5qk 17 4 frameshifting frameshifte VBG cord-298078-uqrwq5qk 17 5 during during IN cord-298078-uqrwq5qk 17 6 replication replication NN cord-298078-uqrwq5qk 17 7 and and CC cord-298078-uqrwq5qk 17 8 generate generate VB cord-298078-uqrwq5qk 17 9 the the DT cord-298078-uqrwq5qk 17 10 frameshifted frameshifte VBN cord-298078-uqrwq5qk 17 11 protein protein NN cord-298078-uqrwq5qk 17 12 critical critical JJ cord-298078-uqrwq5qk 17 13 for for IN cord-298078-uqrwq5qk 17 14 efficient efficient JJ cord-298078-uqrwq5qk 17 15 viral viral JJ cord-298078-uqrwq5qk 17 16 replication replication NN cord-298078-uqrwq5qk 17 17 [ [ -LRB- cord-298078-uqrwq5qk 17 18 2 2 CD cord-298078-uqrwq5qk 17 19 , , , cord-298078-uqrwq5qk 17 20 3 3 CD cord-298078-uqrwq5qk 17 21 , , , cord-298078-uqrwq5qk 17 22 4 4 CD cord-298078-uqrwq5qk 17 23 ] ] -RRB- cord-298078-uqrwq5qk 17 24 . . . cord-298078-uqrwq5qk 18 1 In in IN cord-298078-uqrwq5qk 18 2 the the DT cord-298078-uqrwq5qk 18 3 case case NN cord-298078-uqrwq5qk 18 4 of of IN cord-298078-uqrwq5qk 18 5 retroviruses retrovirus NNS cord-298078-uqrwq5qk 18 6 such such JJ cord-298078-uqrwq5qk 18 7 as as IN cord-298078-uqrwq5qk 18 8 HIV-1 HIV-1 NNP cord-298078-uqrwq5qk 18 9 , , , cord-298078-uqrwq5qk 18 10 ribosomal ribosomal JJ cord-298078-uqrwq5qk 18 11 frameshifting frameshifting NN cord-298078-uqrwq5qk 18 12 is be VBZ cord-298078-uqrwq5qk 18 13 required require VBN cord-298078-uqrwq5qk 18 14 for for IN cord-298078-uqrwq5qk 18 15 the the DT cord-298078-uqrwq5qk 18 16 expression expression NN cord-298078-uqrwq5qk 18 17 of of IN cord-298078-uqrwq5qk 18 18 protease protease NN cord-298078-uqrwq5qk 18 19 , , , cord-298078-uqrwq5qk 18 20 reverse reverse VB cord-298078-uqrwq5qk 18 21 transcriptase transcriptase NN cord-298078-uqrwq5qk 18 22 and and CC cord-298078-uqrwq5qk 18 23 integrase integrase NN cord-298078-uqrwq5qk 18 24 . . . cord-298078-uqrwq5qk 19 1 For for IN cord-298078-uqrwq5qk 19 2 most most JJS cord-298078-uqrwq5qk 19 3 other other JJ cord-298078-uqrwq5qk 19 4 viruses virus NNS cord-298078-uqrwq5qk 19 5 , , , cord-298078-uqrwq5qk 19 6 including include VBG cord-298078-uqrwq5qk 19 7 coronavirus coronavirus NN cord-298078-uqrwq5qk 19 8 avian avian JJ cord-298078-uqrwq5qk 19 9 infectious infectious JJ cord-298078-uqrwq5qk 19 10 bronchitis bronchitis NN cord-298078-uqrwq5qk 19 11 virus virus NN cord-298078-uqrwq5qk 19 12 ( ( -LRB- cord-298078-uqrwq5qk 19 13 IBV IBV NNP cord-298078-uqrwq5qk 19 14 ) ) -RRB- cord-298078-uqrwq5qk 19 15 , , , cord-298078-uqrwq5qk 19 16 frameshifting frameshifting NN cord-298078-uqrwq5qk 19 17 generates generate VBZ cord-298078-uqrwq5qk 19 18 RNA RNA NNP cord-298078-uqrwq5qk 19 19 dependent dependent JJ cord-298078-uqrwq5qk 19 20 RNA RNA NNP cord-298078-uqrwq5qk 19 21 polymerases polymerase NNS cord-298078-uqrwq5qk 19 22 . . . cord-298078-uqrwq5qk 20 1 Since since IN cord-298078-uqrwq5qk 20 2 translation translation NN cord-298078-uqrwq5qk 20 3 is be VBZ cord-298078-uqrwq5qk 20 4 a a DT cord-298078-uqrwq5qk 20 5 complex complex JJ cord-298078-uqrwq5qk 20 6 process process NN cord-298078-uqrwq5qk 20 7 involving involve VBG cord-298078-uqrwq5qk 20 8 many many JJ cord-298078-uqrwq5qk 20 9 regulatory regulatory JJ cord-298078-uqrwq5qk 20 10 factors factor NNS cord-298078-uqrwq5qk 20 11 in in IN cord-298078-uqrwq5qk 20 12 addition addition NN cord-298078-uqrwq5qk 20 13 to to IN cord-298078-uqrwq5qk 20 14 the the DT cord-298078-uqrwq5qk 20 15 ribosome ribosome NN cord-298078-uqrwq5qk 20 16 , , , cord-298078-uqrwq5qk 20 17 binding bind VBG cord-298078-uqrwq5qk 20 18 of of IN cord-298078-uqrwq5qk 20 19 RNA RNA NNP cord-298078-uqrwq5qk 20 20 binding bind VBG cord-298078-uqrwq5qk 20 21 protein protein NN cord-298078-uqrwq5qk 20 22 ( ( -LRB- cord-298078-uqrwq5qk 20 23 RBP RBP NNP cord-298078-uqrwq5qk 20 24 ) ) -RRB- cord-298078-uqrwq5qk 20 25 at at IN cord-298078-uqrwq5qk 20 26 a a DT cord-298078-uqrwq5qk 20 27 nearby nearby JJ cord-298078-uqrwq5qk 20 28 RNA RNA NNP cord-298078-uqrwq5qk 20 29 signal signal NN cord-298078-uqrwq5qk 20 30 could could MD cord-298078-uqrwq5qk 20 31 affect affect VB cord-298078-uqrwq5qk 20 32 RNA RNA NNP cord-298078-uqrwq5qk 20 33 conformation conformation NN cord-298078-uqrwq5qk 20 34 and and CC cord-298078-uqrwq5qk 20 35 somehow somehow RB cord-298078-uqrwq5qk 20 36 redirect redirect VB cord-298078-uqrwq5qk 20 37 the the DT cord-298078-uqrwq5qk 20 38 translational translational JJ cord-298078-uqrwq5qk 20 39 machinery machinery NN cord-298078-uqrwq5qk 20 40 [ [ -LRB- cord-298078-uqrwq5qk 20 41 5 5 CD cord-298078-uqrwq5qk 20 42 , , , cord-298078-uqrwq5qk 20 43 6 6 CD cord-298078-uqrwq5qk 20 44 ] ] -RRB- cord-298078-uqrwq5qk 20 45 . . . cord-298078-uqrwq5qk 21 1 Therefore therefore RB cord-298078-uqrwq5qk 21 2 , , , cord-298078-uqrwq5qk 21 3 the the DT cord-298078-uqrwq5qk 21 4 identification identification NN cord-298078-uqrwq5qk 21 5 of of IN cord-298078-uqrwq5qk 21 6 RBPs rbp NNS cord-298078-uqrwq5qk 21 7 involved involve VBN cord-298078-uqrwq5qk 21 8 in in IN cord-298078-uqrwq5qk 21 9 frameshifting frameshifte VBG cord-298078-uqrwq5qk 21 10 is be VBZ cord-298078-uqrwq5qk 21 11 important important JJ cord-298078-uqrwq5qk 21 12 in in IN cord-298078-uqrwq5qk 21 13 elucidating elucidate VBG cord-298078-uqrwq5qk 21 14 the the DT cord-298078-uqrwq5qk 21 15 regulatory regulatory JJ cord-298078-uqrwq5qk 21 16 mechanism mechanism NN cord-298078-uqrwq5qk 21 17 for for IN cord-298078-uqrwq5qk 21 18 RNA RNA NNP cord-298078-uqrwq5qk 21 19 translation translation NN cord-298078-uqrwq5qk 21 20 . . . cord-298078-uqrwq5qk 22 1 Viral viral JJ cord-298078-uqrwq5qk 22 2 frameshifting frameshifting NN cord-298078-uqrwq5qk 22 3 signals signal NNS cord-298078-uqrwq5qk 22 4 are be VBP cord-298078-uqrwq5qk 22 5 present present JJ cord-298078-uqrwq5qk 22 6 in in IN cord-298078-uqrwq5qk 22 7 coding code VBG cord-298078-uqrwq5qk 22 8 regions region NNS cord-298078-uqrwq5qk 22 9 of of IN cord-298078-uqrwq5qk 22 10 mRNA mrna NN cord-298078-uqrwq5qk 22 11 , , , cord-298078-uqrwq5qk 22 12 and and CC cord-298078-uqrwq5qk 22 13 are be VBP cord-298078-uqrwq5qk 22 14 generally generally RB cord-298078-uqrwq5qk 22 15 composed compose VBN cord-298078-uqrwq5qk 22 16 of of IN cord-298078-uqrwq5qk 22 17 a a DT cord-298078-uqrwq5qk 22 18 ' ' `` cord-298078-uqrwq5qk 22 19 ' ' `` cord-298078-uqrwq5qk 22 20 slippery slippery JJ cord-298078-uqrwq5qk 22 21 ' ' '' cord-298078-uqrwq5qk 22 22 ' ' '' cord-298078-uqrwq5qk 22 23 sequence sequence NN cord-298078-uqrwq5qk 22 24 , , , cord-298078-uqrwq5qk 22 25 a a DT cord-298078-uqrwq5qk 22 26 stimulatory stimulatory JJ cord-298078-uqrwq5qk 22 27 downstream downstream JJ cord-298078-uqrwq5qk 22 28 RNA RNA NNP cord-298078-uqrwq5qk 22 29 hairpin hairpin NN cord-298078-uqrwq5qk 22 30 or or CC cord-298078-uqrwq5qk 22 31 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 22 32 structure structure NN cord-298078-uqrwq5qk 22 33 and and CC cord-298078-uqrwq5qk 22 34 an an DT cord-298078-uqrwq5qk 22 35 intervening intervene VBG cord-298078-uqrwq5qk 22 36 spacer spacer NN cord-298078-uqrwq5qk 22 37 region region NN cord-298078-uqrwq5qk 22 38 [ [ -LRB- cord-298078-uqrwq5qk 22 39 7 7 CD cord-298078-uqrwq5qk 22 40 , , , cord-298078-uqrwq5qk 22 41 8 8 CD cord-298078-uqrwq5qk 22 42 , , , cord-298078-uqrwq5qk 22 43 9 9 CD cord-298078-uqrwq5qk 22 44 ] ] -RRB- cord-298078-uqrwq5qk 22 45 . . . cord-298078-uqrwq5qk 23 1 RNA RNA NNP cord-298078-uqrwq5qk 23 2 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 23 3 are be VBP cord-298078-uqrwq5qk 23 4 structural structural JJ cord-298078-uqrwq5qk 23 5 elements element NNS cord-298078-uqrwq5qk 23 6 found find VBN cord-298078-uqrwq5qk 23 7 in in IN cord-298078-uqrwq5qk 23 8 almost almost RB cord-298078-uqrwq5qk 23 9 all all DT cord-298078-uqrwq5qk 23 10 classes class NNS cord-298078-uqrwq5qk 23 11 of of IN cord-298078-uqrwq5qk 23 12 RNA RNA NNP cord-298078-uqrwq5qk 23 13 , , , cord-298078-uqrwq5qk 23 14 which which WDT cord-298078-uqrwq5qk 23 15 are be VBP cord-298078-uqrwq5qk 23 16 now now RB cord-298078-uqrwq5qk 23 17 recognized recognize VBN cord-298078-uqrwq5qk 23 18 as as IN cord-298078-uqrwq5qk 23 19 a a DT cord-298078-uqrwq5qk 23 20 widespread widespread JJ cord-298078-uqrwq5qk 23 21 motif motif NN cord-298078-uqrwq5qk 23 22 with with IN cord-298078-uqrwq5qk 23 23 diverse diverse JJ cord-298078-uqrwq5qk 23 24 biological biological JJ cord-298078-uqrwq5qk 23 25 functions function NNS cord-298078-uqrwq5qk 23 26 [ [ -LRB- cord-298078-uqrwq5qk 23 27 2 2 CD cord-298078-uqrwq5qk 23 28 ] ] -RRB- cord-298078-uqrwq5qk 23 29 . . . cord-298078-uqrwq5qk 24 1 For for IN cord-298078-uqrwq5qk 24 2 example example NN cord-298078-uqrwq5qk 24 3 , , , cord-298078-uqrwq5qk 24 4 viral viral JJ cord-298078-uqrwq5qk 24 5 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 24 6 in in IN cord-298078-uqrwq5qk 24 7 non non JJ cord-298078-uqrwq5qk 24 8 - - JJ cord-298078-uqrwq5qk 24 9 coding coding JJ cord-298078-uqrwq5qk 24 10 regions region NNS cord-298078-uqrwq5qk 24 11 act act VBP cord-298078-uqrwq5qk 24 12 in in IN cord-298078-uqrwq5qk 24 13 the the DT cord-298078-uqrwq5qk 24 14 regulation regulation NN cord-298078-uqrwq5qk 24 15 of of IN cord-298078-uqrwq5qk 24 16 translation translation NN cord-298078-uqrwq5qk 24 17 initiation initiation NN cord-298078-uqrwq5qk 24 18 and and CC cord-298078-uqrwq5qk 24 19 in in IN cord-298078-uqrwq5qk 24 20 template template NN cord-298078-uqrwq5qk 24 21 recognition recognition NN cord-298078-uqrwq5qk 24 22 by by IN cord-298078-uqrwq5qk 24 23 viral viral JJ cord-298078-uqrwq5qk 24 24 replicase replicase NN cord-298078-uqrwq5qk 24 25 . . . cord-298078-uqrwq5qk 25 1 In in IN cord-298078-uqrwq5qk 25 2 contrast contrast NN cord-298078-uqrwq5qk 25 3 , , , cord-298078-uqrwq5qk 25 4 when when WRB cord-298078-uqrwq5qk 25 5 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 25 6 are be VBP cord-298078-uqrwq5qk 25 7 present present JJ cord-298078-uqrwq5qk 25 8 in in IN cord-298078-uqrwq5qk 25 9 coding code VBG cord-298078-uqrwq5qk 25 10 regions region NNS cord-298078-uqrwq5qk 25 11 , , , cord-298078-uqrwq5qk 25 12 they -PRON- PRP cord-298078-uqrwq5qk 25 13 regulate regulate VBP cord-298078-uqrwq5qk 25 14 translation translation NN cord-298078-uqrwq5qk 25 15 elongation elongation NN cord-298078-uqrwq5qk 25 16 and and CC cord-298078-uqrwq5qk 25 17 termination termination NN cord-298078-uqrwq5qk 25 18 , , , cord-298078-uqrwq5qk 25 19 leading lead VBG cord-298078-uqrwq5qk 25 20 to to IN cord-298078-uqrwq5qk 25 21 ribosomal ribosomal JJ cord-298078-uqrwq5qk 25 22 frameshifting frameshifte VBG cord-298078-uqrwq5qk 25 23 [ [ -LRB- cord-298078-uqrwq5qk 25 24 10 10 CD cord-298078-uqrwq5qk 25 25 ] ] -RRB- cord-298078-uqrwq5qk 25 26 . . . cord-298078-uqrwq5qk 26 1 It -PRON- PRP cord-298078-uqrwq5qk 26 2 is be VBZ cord-298078-uqrwq5qk 26 3 not not RB cord-298078-uqrwq5qk 26 4 clear clear JJ cord-298078-uqrwq5qk 26 5 whether whether IN cord-298078-uqrwq5qk 26 6 ribosomal ribosomal JJ cord-298078-uqrwq5qk 26 7 frameshifting frameshifting NN cord-298078-uqrwq5qk 26 8 occurs occur VBZ cord-298078-uqrwq5qk 26 9 through through IN cord-298078-uqrwq5qk 26 10 the the DT cord-298078-uqrwq5qk 26 11 interaction interaction NN cord-298078-uqrwq5qk 26 12 of of IN cord-298078-uqrwq5qk 26 13 ribosome ribosome NN cord-298078-uqrwq5qk 26 14 or or CC cord-298078-uqrwq5qk 26 15 any any DT cord-298078-uqrwq5qk 26 16 RBPs rbps NN cord-298078-uqrwq5qk 26 17 , , , cord-298078-uqrwq5qk 26 18 or or CC cord-298078-uqrwq5qk 26 19 due due IN cord-298078-uqrwq5qk 26 20 to to IN cord-298078-uqrwq5qk 26 21 the the DT cord-298078-uqrwq5qk 26 22 intrinsic intrinsic JJ cord-298078-uqrwq5qk 26 23 nature nature NN cord-298078-uqrwq5qk 26 24 of of IN cord-298078-uqrwq5qk 26 25 the the DT cord-298078-uqrwq5qk 26 26 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 26 27 RNA RNA NNP cord-298078-uqrwq5qk 26 28 . . . cord-298078-uqrwq5qk 27 1 Annexin Annexin NNP cord-298078-uqrwq5qk 27 2 A2 A2 NNP cord-298078-uqrwq5qk 27 3 ( ( -LRB- cord-298078-uqrwq5qk 27 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 27 5 ) ) -RRB- cord-298078-uqrwq5qk 27 6 is be VBZ cord-298078-uqrwq5qk 27 7 a a DT cord-298078-uqrwq5qk 27 8 multi multi JJ cord-298078-uqrwq5qk 27 9 - - JJ cord-298078-uqrwq5qk 27 10 functional functional JJ cord-298078-uqrwq5qk 27 11 protein protein NN cord-298078-uqrwq5qk 27 12 that that WDT cord-298078-uqrwq5qk 27 13 has have VBZ cord-298078-uqrwq5qk 27 14 been be VBN cord-298078-uqrwq5qk 27 15 implicated implicate VBN cord-298078-uqrwq5qk 27 16 in in IN cord-298078-uqrwq5qk 27 17 a a DT cord-298078-uqrwq5qk 27 18 number number NN cord-298078-uqrwq5qk 27 19 of of IN cord-298078-uqrwq5qk 27 20 cellular cellular JJ cord-298078-uqrwq5qk 27 21 functions function NNS cord-298078-uqrwq5qk 27 22 , , , cord-298078-uqrwq5qk 27 23 including include VBG cord-298078-uqrwq5qk 27 24 calcium calcium NN cord-298078-uqrwq5qk 27 25 dependent dependent JJ cord-298078-uqrwq5qk 27 26 regulation regulation NN cord-298078-uqrwq5qk 27 27 of of IN cord-298078-uqrwq5qk 27 28 exocytosis exocytosis NN cord-298078-uqrwq5qk 27 29 , , , cord-298078-uqrwq5qk 27 30 DNA dna NN cord-298078-uqrwq5qk 27 31 synthesis synthesis NN cord-298078-uqrwq5qk 27 32 and and CC cord-298078-uqrwq5qk 27 33 cell cell NN cord-298078-uqrwq5qk 27 34 proliferation proliferation NN cord-298078-uqrwq5qk 27 35 [ [ -LRB- cord-298078-uqrwq5qk 27 36 11 11 CD cord-298078-uqrwq5qk 27 37 ] ] -RRB- cord-298078-uqrwq5qk 27 38 . . . cord-298078-uqrwq5qk 28 1 Recently recently RB cord-298078-uqrwq5qk 28 2 , , , cord-298078-uqrwq5qk 28 3 its -PRON- PRP$ cord-298078-uqrwq5qk 28 4 potential potential JJ cord-298078-uqrwq5qk 28 5 role role NN cord-298078-uqrwq5qk 28 6 in in IN cord-298078-uqrwq5qk 28 7 RNA RNA NNP cord-298078-uqrwq5qk 28 8 metabolism metabolism NN cord-298078-uqrwq5qk 28 9 was be VBD cord-298078-uqrwq5qk 28 10 proposed propose VBN cord-298078-uqrwq5qk 28 11 because because IN cord-298078-uqrwq5qk 28 12 it -PRON- PRP cord-298078-uqrwq5qk 28 13 was be VBD cord-298078-uqrwq5qk 28 14 found find VBN cord-298078-uqrwq5qk 28 15 to to TO cord-298078-uqrwq5qk 28 16 bind bind VB cord-298078-uqrwq5qk 28 17 some some DT cord-298078-uqrwq5qk 28 18 mRNA mRNA NNP cord-298078-uqrwq5qk 28 19 transcripts transcript NNS cord-298078-uqrwq5qk 28 20 , , , cord-298078-uqrwq5qk 28 21 such such JJ cord-298078-uqrwq5qk 28 22 as as IN cord-298078-uqrwq5qk 28 23 c c NNP cord-298078-uqrwq5qk 28 24 - - HYPH cord-298078-uqrwq5qk 28 25 myc myc NNP cord-298078-uqrwq5qk 28 26 , , , cord-298078-uqrwq5qk 28 27 collagen collagen NNP cord-298078-uqrwq5qk 28 28 prolyl prolyl NNP cord-298078-uqrwq5qk 28 29 4-hydroxylasea 4-hydroxylasea CD cord-298078-uqrwq5qk 28 30 ( ( -LRB- cord-298078-uqrwq5qk 28 31 I i NN cord-298078-uqrwq5qk 28 32 ) ) -RRB- cord-298078-uqrwq5qk 28 33 and and CC cord-298078-uqrwq5qk 28 34 its -PRON- PRP$ cord-298078-uqrwq5qk 28 35 own own JJ cord-298078-uqrwq5qk 28 36 mRNA mrna NN cord-298078-uqrwq5qk 28 37 [ [ -LRB- cord-298078-uqrwq5qk 28 38 12 12 CD cord-298078-uqrwq5qk 28 39 , , , cord-298078-uqrwq5qk 28 40 13 13 CD cord-298078-uqrwq5qk 28 41 , , , cord-298078-uqrwq5qk 28 42 14 14 CD cord-298078-uqrwq5qk 28 43 , , , cord-298078-uqrwq5qk 28 44 15 15 CD cord-298078-uqrwq5qk 28 45 , , , cord-298078-uqrwq5qk 28 46 16 16 CD cord-298078-uqrwq5qk 28 47 ] ] -RRB- cord-298078-uqrwq5qk 28 48 . . . cord-298078-uqrwq5qk 29 1 Since since IN cord-298078-uqrwq5qk 29 2 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 29 3 binds bind VBZ cord-298078-uqrwq5qk 29 4 39untranslated 39untranslated CD cord-298078-uqrwq5qk 29 5 region region NN cord-298078-uqrwq5qk 29 6 ( ( -LRB- cord-298078-uqrwq5qk 29 7 UTR UTR NNP cord-298078-uqrwq5qk 29 8 ) ) -RRB- cord-298078-uqrwq5qk 29 9 of of IN cord-298078-uqrwq5qk 29 10 these these DT cord-298078-uqrwq5qk 29 11 mRNAs mRNAs NNPS cord-298078-uqrwq5qk 29 12 , , , cord-298078-uqrwq5qk 29 13 it -PRON- PRP cord-298078-uqrwq5qk 29 14 was be VBD cord-298078-uqrwq5qk 29 15 suggested suggest VBN cord-298078-uqrwq5qk 29 16 to to TO cord-298078-uqrwq5qk 29 17 be be VB cord-298078-uqrwq5qk 29 18 involved involve VBN cord-298078-uqrwq5qk 29 19 in in IN cord-298078-uqrwq5qk 29 20 mRNA mRNA NNP cord-298078-uqrwq5qk 29 21 localization localization NN cord-298078-uqrwq5qk 29 22 and and CC cord-298078-uqrwq5qk 29 23 translational translational JJ cord-298078-uqrwq5qk 29 24 regulation regulation NN cord-298078-uqrwq5qk 29 25 [ [ -LRB- cord-298078-uqrwq5qk 29 26 15 15 CD cord-298078-uqrwq5qk 29 27 , , , cord-298078-uqrwq5qk 29 28 17 17 CD cord-298078-uqrwq5qk 29 29 ] ] -RRB- cord-298078-uqrwq5qk 29 30 . . . cord-298078-uqrwq5qk 30 1 Interestingly interestingly RB cord-298078-uqrwq5qk 30 2 , , , cord-298078-uqrwq5qk 30 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 30 4 is be VBZ cord-298078-uqrwq5qk 30 5 reported report VBN cord-298078-uqrwq5qk 30 6 to to TO cord-298078-uqrwq5qk 30 7 be be VB cord-298078-uqrwq5qk 30 8 the the DT cord-298078-uqrwq5qk 30 9 most most RBS cord-298078-uqrwq5qk 30 10 abundant abundant JJ cord-298078-uqrwq5qk 30 11 proteins protein NNS cord-298078-uqrwq5qk 30 12 in in IN cord-298078-uqrwq5qk 30 13 cytoskeleton cytoskeleton NN cord-298078-uqrwq5qk 30 14 bound bind VBN cord-298078-uqrwq5qk 30 15 polyribosome polyribosome NNP cord-298078-uqrwq5qk 30 16 fraction fraction NN cord-298078-uqrwq5qk 30 17 and and CC cord-298078-uqrwq5qk 30 18 also also RB cord-298078-uqrwq5qk 30 19 associated associate VBN cord-298078-uqrwq5qk 30 20 with with IN cord-298078-uqrwq5qk 30 21 mRNAs mrnas PRP cord-298078-uqrwq5qk 30 22 [ [ -LRB- cord-298078-uqrwq5qk 30 23 17 17 CD cord-298078-uqrwq5qk 30 24 ] ] -RRB- cord-298078-uqrwq5qk 30 25 . . . cord-298078-uqrwq5qk 31 1 These these DT cord-298078-uqrwq5qk 31 2 findings finding NNS cord-298078-uqrwq5qk 31 3 led lead VBD cord-298078-uqrwq5qk 31 4 to to IN cord-298078-uqrwq5qk 31 5 the the DT cord-298078-uqrwq5qk 31 6 suggestion suggestion NN cord-298078-uqrwq5qk 31 7 that that IN cord-298078-uqrwq5qk 31 8 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 31 9 may may MD cord-298078-uqrwq5qk 31 10 bind bind VB cord-298078-uqrwq5qk 31 11 to to IN cord-298078-uqrwq5qk 31 12 some some DT cord-298078-uqrwq5qk 31 13 RNA RNA NNP cord-298078-uqrwq5qk 31 14 elements element NNS cord-298078-uqrwq5qk 31 15 involved involve VBN cord-298078-uqrwq5qk 31 16 in in IN cord-298078-uqrwq5qk 31 17 translational translational JJ cord-298078-uqrwq5qk 31 18 regulation regulation NN cord-298078-uqrwq5qk 31 19 . . . cord-298078-uqrwq5qk 32 1 In in IN cord-298078-uqrwq5qk 32 2 fact fact NN cord-298078-uqrwq5qk 32 3 , , , cord-298078-uqrwq5qk 32 4 aberrant aberrant JJ cord-298078-uqrwq5qk 32 5 expression expression NN cord-298078-uqrwq5qk 32 6 of of IN cord-298078-uqrwq5qk 32 7 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 32 8 is be VBZ cord-298078-uqrwq5qk 32 9 somehow somehow RB cord-298078-uqrwq5qk 32 10 related relate VBN cord-298078-uqrwq5qk 32 11 to to IN cord-298078-uqrwq5qk 32 12 the the DT cord-298078-uqrwq5qk 32 13 carcinogenesis carcinogenesis NN cord-298078-uqrwq5qk 32 14 of of IN cord-298078-uqrwq5qk 32 15 human human JJ cord-298078-uqrwq5qk 32 16 cancers cancer NNS cord-298078-uqrwq5qk 32 17 , , , cord-298078-uqrwq5qk 32 18 because because IN cord-298078-uqrwq5qk 32 19 it -PRON- PRP cord-298078-uqrwq5qk 32 20 has have VBZ cord-298078-uqrwq5qk 32 21 been be VBN cord-298078-uqrwq5qk 32 22 shown show VBN cord-298078-uqrwq5qk 32 23 to to TO cord-298078-uqrwq5qk 32 24 be be VB cord-298078-uqrwq5qk 32 25 clearly clearly RB cord-298078-uqrwq5qk 32 26 absent absent JJ cord-298078-uqrwq5qk 32 27 in in IN cord-298078-uqrwq5qk 32 28 some some DT cord-298078-uqrwq5qk 32 29 prostate prostate NN cord-298078-uqrwq5qk 32 30 cancers cancer NNS cord-298078-uqrwq5qk 32 31 and and CC cord-298078-uqrwq5qk 32 32 highly highly RB cord-298078-uqrwq5qk 32 33 overexpressed overexpresse VBN cord-298078-uqrwq5qk 32 34 in in IN cord-298078-uqrwq5qk 32 35 other other JJ cord-298078-uqrwq5qk 32 36 human human JJ cord-298078-uqrwq5qk 32 37 cancers cancer NNS cord-298078-uqrwq5qk 32 38 [ [ -LRB- cord-298078-uqrwq5qk 32 39 18 18 CD cord-298078-uqrwq5qk 32 40 , , , cord-298078-uqrwq5qk 32 41 19 19 CD cord-298078-uqrwq5qk 32 42 , , , cord-298078-uqrwq5qk 32 43 20 20 CD cord-298078-uqrwq5qk 32 44 ] ] -RRB- cord-298078-uqrwq5qk 32 45 . . . cord-298078-uqrwq5qk 33 1 To to TO cord-298078-uqrwq5qk 33 2 identify identify VB cord-298078-uqrwq5qk 33 3 the the DT cord-298078-uqrwq5qk 33 4 proteins protein NNS cord-298078-uqrwq5qk 33 5 that that WDT cord-298078-uqrwq5qk 33 6 might may MD cord-298078-uqrwq5qk 33 7 recognize recognize VB cord-298078-uqrwq5qk 33 8 frameshifting frameshifting JJ cord-298078-uqrwq5qk 33 9 signals signal NNS cord-298078-uqrwq5qk 33 10 , , , cord-298078-uqrwq5qk 33 11 we -PRON- PRP cord-298078-uqrwq5qk 33 12 presently presently RB cord-298078-uqrwq5qk 33 13 utilized utilize VBD cord-298078-uqrwq5qk 33 14 IBV IBV NNP cord-298078-uqrwq5qk 33 15 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 33 16 RNA RNA NNP cord-298078-uqrwq5qk 33 17 as as IN cord-298078-uqrwq5qk 33 18 a a DT cord-298078-uqrwq5qk 33 19 model model NN cord-298078-uqrwq5qk 33 20 transcript transcript NN cord-298078-uqrwq5qk 33 21 . . . cord-298078-uqrwq5qk 34 1 We -PRON- PRP cord-298078-uqrwq5qk 34 2 identified identify VBD cord-298078-uqrwq5qk 34 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 34 4 as as IN cord-298078-uqrwq5qk 34 5 a a DT cord-298078-uqrwq5qk 34 6 RBP rbp NN cord-298078-uqrwq5qk 34 7 that that WDT cord-298078-uqrwq5qk 34 8 can can MD cord-298078-uqrwq5qk 34 9 recognize recognize VB cord-298078-uqrwq5qk 34 10 viral viral JJ cord-298078-uqrwq5qk 34 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 34 12 RNA RNA NNP cord-298078-uqrwq5qk 34 13 and and CC cord-298078-uqrwq5qk 34 14 reduce reduce VB cord-298078-uqrwq5qk 34 15 viral viral JJ cord-298078-uqrwq5qk 34 16 frameshifting frameshifting NN cord-298078-uqrwq5qk 34 17 . . . cord-298078-uqrwq5qk 35 1 We -PRON- PRP cord-298078-uqrwq5qk 35 2 confirmed confirm VBD cord-298078-uqrwq5qk 35 3 the the DT cord-298078-uqrwq5qk 35 4 RNA RNA NNP cord-298078-uqrwq5qk 35 5 binding binding NN cord-298078-uqrwq5qk 35 6 activity activity NN cord-298078-uqrwq5qk 35 7 of of IN cord-298078-uqrwq5qk 35 8 ANXA2 anxa2 NN cord-298078-uqrwq5qk 35 9 by by IN cord-298078-uqrwq5qk 35 10 various various JJ cord-298078-uqrwq5qk 35 11 methods method NNS cord-298078-uqrwq5qk 35 12 and and CC cord-298078-uqrwq5qk 35 13 tested test VBD cord-298078-uqrwq5qk 35 14 its -PRON- PRP$ cord-298078-uqrwq5qk 35 15 role role NN cord-298078-uqrwq5qk 35 16 in in IN cord-298078-uqrwq5qk 35 17 the the DT cord-298078-uqrwq5qk 35 18 regulation regulation NN cord-298078-uqrwq5qk 35 19 of of IN cord-298078-uqrwq5qk 35 20 frameshifting frameshifting NN cord-298078-uqrwq5qk 35 21 . . . cord-298078-uqrwq5qk 36 1 By by IN cord-298078-uqrwq5qk 36 2 overexpression overexpression NN cord-298078-uqrwq5qk 36 3 and and CC cord-298078-uqrwq5qk 36 4 knockdown knockdown NN cord-298078-uqrwq5qk 36 5 approaches approach NNS cord-298078-uqrwq5qk 36 6 , , , cord-298078-uqrwq5qk 36 7 we -PRON- PRP cord-298078-uqrwq5qk 36 8 showed show VBD cord-298078-uqrwq5qk 36 9 it -PRON- PRP cord-298078-uqrwq5qk 36 10 to to TO cord-298078-uqrwq5qk 36 11 be be VB cord-298078-uqrwq5qk 36 12 an an DT cord-298078-uqrwq5qk 36 13 efficient efficient JJ cord-298078-uqrwq5qk 36 14 modulator modulator NN cord-298078-uqrwq5qk 36 15 of of IN cord-298078-uqrwq5qk 36 16 viral viral JJ cord-298078-uqrwq5qk 36 17 frameshifting frameshifting NN cord-298078-uqrwq5qk 36 18 . . . cord-298078-uqrwq5qk 37 1 We -PRON- PRP cord-298078-uqrwq5qk 37 2 speculate speculate VBP cord-298078-uqrwq5qk 37 3 that that IN cord-298078-uqrwq5qk 37 4 cellular cellular JJ cord-298078-uqrwq5qk 37 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 37 6 protein protein NN cord-298078-uqrwq5qk 37 7 may may MD cord-298078-uqrwq5qk 37 8 act act VB cord-298078-uqrwq5qk 37 9 as as IN cord-298078-uqrwq5qk 37 10 an an DT cord-298078-uqrwq5qk 37 11 anti anti JJ cord-298078-uqrwq5qk 37 12 - - JJ cord-298078-uqrwq5qk 37 13 viral viral JJ cord-298078-uqrwq5qk 37 14 protein protein NN cord-298078-uqrwq5qk 37 15 that that WDT cord-298078-uqrwq5qk 37 16 can can MD cord-298078-uqrwq5qk 37 17 reduce reduce VB cord-298078-uqrwq5qk 37 18 detrimental detrimental JJ cord-298078-uqrwq5qk 37 19 viral viral JJ cord-298078-uqrwq5qk 37 20 frameshifting frameshifte VBG cord-298078-uqrwq5qk 37 21 and and CC cord-298078-uqrwq5qk 37 22 successful successful JJ cord-298078-uqrwq5qk 37 23 replication replication NN cord-298078-uqrwq5qk 37 24 of of IN cord-298078-uqrwq5qk 37 25 the the DT cord-298078-uqrwq5qk 37 26 virus virus NN cord-298078-uqrwq5qk 37 27 . . . cord-298078-uqrwq5qk 38 1 The the DT cord-298078-uqrwq5qk 38 2 present present JJ cord-298078-uqrwq5qk 38 3 results result NNS cord-298078-uqrwq5qk 38 4 offer offer VBP cord-298078-uqrwq5qk 38 5 a a DT cord-298078-uqrwq5qk 38 6 clue clue NN cord-298078-uqrwq5qk 38 7 for for IN cord-298078-uqrwq5qk 38 8 the the DT cord-298078-uqrwq5qk 38 9 role role NN cord-298078-uqrwq5qk 38 10 of of IN cord-298078-uqrwq5qk 38 11 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 38 12 as as IN cord-298078-uqrwq5qk 38 13 a a DT cord-298078-uqrwq5qk 38 14 RBP rbp NN cord-298078-uqrwq5qk 38 15 for for IN cord-298078-uqrwq5qk 38 16 other other JJ cord-298078-uqrwq5qk 38 17 cellular cellular JJ cord-298078-uqrwq5qk 38 18 RNA RNA NNP cord-298078-uqrwq5qk 38 19 transcripts transcript NNS cord-298078-uqrwq5qk 38 20 . . . cord-298078-uqrwq5qk 39 1 Since since IN cord-298078-uqrwq5qk 39 2 IBV IBV NNP cord-298078-uqrwq5qk 39 3 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 39 4 RNA RNA NNP cord-298078-uqrwq5qk 39 5 was be VBD cord-298078-uqrwq5qk 39 6 shown show VBN cord-298078-uqrwq5qk 39 7 to to TO cord-298078-uqrwq5qk 39 8 induce induce VB cord-298078-uqrwq5qk 39 9 ribosomal ribosomal JJ cord-298078-uqrwq5qk 39 10 frameshifting frameshifte VBG cord-298078-uqrwq5qk 39 11 during during IN cord-298078-uqrwq5qk 39 12 RNA RNA NNP cord-298078-uqrwq5qk 39 13 translation translation NN cord-298078-uqrwq5qk 39 14 , , , cord-298078-uqrwq5qk 39 15 we -PRON- PRP cord-298078-uqrwq5qk 39 16 utilized utilize VBD cord-298078-uqrwq5qk 39 17 IBV IBV NNP cord-298078-uqrwq5qk 39 18 frameshifting frameshifte VBG cord-298078-uqrwq5qk 39 19 RNA RNA NNP cord-298078-uqrwq5qk 39 20 sequences sequence NNS cord-298078-uqrwq5qk 39 21 as as IN cord-298078-uqrwq5qk 39 22 the the DT cord-298078-uqrwq5qk 39 23 model model NN cord-298078-uqrwq5qk 39 24 transcript transcript NN cord-298078-uqrwq5qk 39 25 [ [ -LRB- cord-298078-uqrwq5qk 39 26 3 3 CD cord-298078-uqrwq5qk 39 27 ] ] -RRB- cord-298078-uqrwq5qk 39 28 . . . cord-298078-uqrwq5qk 40 1 IBV IBV NNP cord-298078-uqrwq5qk 40 2 genomic genomic JJ cord-298078-uqrwq5qk 40 3 RNA RNA NNP cord-298078-uqrwq5qk 40 4 contains contain VBZ cord-298078-uqrwq5qk 40 5 the the DT cord-298078-uqrwq5qk 40 6 frameshifting frameshifte VBG cord-298078-uqrwq5qk 40 7 RNA RNA NNP cord-298078-uqrwq5qk 40 8 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 40 9 as as RB cord-298078-uqrwq5qk 40 10 well well RB cord-298078-uqrwq5qk 40 11 as as IN cord-298078-uqrwq5qk 40 12 the the DT cord-298078-uqrwq5qk 40 13 slippery slippery JJ cord-298078-uqrwq5qk 40 14 site site NN cord-298078-uqrwq5qk 40 15 and and CC cord-298078-uqrwq5qk 40 16 the the DT cord-298078-uqrwq5qk 40 17 spacer spacer NN cord-298078-uqrwq5qk 40 18 . . . cord-298078-uqrwq5qk 41 1 Sequences sequence NNS cord-298078-uqrwq5qk 41 2 of of IN cord-298078-uqrwq5qk 41 3 wild wild JJ cord-298078-uqrwq5qk 41 4 - - HYPH cord-298078-uqrwq5qk 41 5 type type NN cord-298078-uqrwq5qk 41 6 and and CC cord-298078-uqrwq5qk 41 7 mutant mutant JJ cord-298078-uqrwq5qk 41 8 IBV IBV NNP cord-298078-uqrwq5qk 41 9 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 41 10 RNA RNA NNP cord-298078-uqrwq5qk 41 11 ( ( -LRB- cord-298078-uqrwq5qk 41 12 72 72 CD cord-298078-uqrwq5qk 41 13 nts nt NNS cord-298078-uqrwq5qk 41 14 ) ) -RRB- cord-298078-uqrwq5qk 41 15 shown show VBN cord-298078-uqrwq5qk 41 16 in in IN cord-298078-uqrwq5qk 41 17 Figure figure NN cord-298078-uqrwq5qk 41 18 1A 1a NN cord-298078-uqrwq5qk 41 19 were be VBD cord-298078-uqrwq5qk 41 20 generated generate VBN cord-298078-uqrwq5qk 41 21 as as IN cord-298078-uqrwq5qk 41 22 in in IN cord-298078-uqrwq5qk 41 23 vitro vitro FW cord-298078-uqrwq5qk 41 24 transcribed transcribe VBN cord-298078-uqrwq5qk 41 25 transcript transcript NN cord-298078-uqrwq5qk 41 26 . . . cord-298078-uqrwq5qk 42 1 RNA RNA NNP cord-298078-uqrwq5qk 42 2 structure structure NN cord-298078-uqrwq5qk 42 3 of of IN cord-298078-uqrwq5qk 42 4 wild wild JJ cord-298078-uqrwq5qk 42 5 - - HYPH cord-298078-uqrwq5qk 42 6 type type NN cord-298078-uqrwq5qk 42 7 IBV IBV NNP cord-298078-uqrwq5qk 42 8 RNA RNA NNP cord-298078-uqrwq5qk 42 9 was be VBD cord-298078-uqrwq5qk 42 10 predicted predict VBN cord-298078-uqrwq5qk 42 11 to to TO cord-298078-uqrwq5qk 42 12 fold fold VB cord-298078-uqrwq5qk 42 13 into into IN cord-298078-uqrwq5qk 42 14 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 42 15 structure structure NN cord-298078-uqrwq5qk 42 16 , , , cord-298078-uqrwq5qk 42 17 whereas whereas IN cord-298078-uqrwq5qk 42 18 mutant mutant JJ cord-298078-uqrwq5qk 42 19 IBV IBV NNP cord-298078-uqrwq5qk 42 20 RNA RNA NNP cord-298078-uqrwq5qk 42 21 was be VBD cord-298078-uqrwq5qk 42 22 not not RB cord-298078-uqrwq5qk 42 23 able able JJ cord-298078-uqrwq5qk 42 24 to to TO cord-298078-uqrwq5qk 42 25 form form VB cord-298078-uqrwq5qk 42 26 a a DT cord-298078-uqrwq5qk 42 27 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 42 28 structure structure NN cord-298078-uqrwq5qk 42 29 ( ( -LRB- cord-298078-uqrwq5qk 42 30 pknotsRG pknotsRG NNP cord-298078-uqrwq5qk 42 31 : : : cord-298078-uqrwq5qk 42 32 http://bibiserv.techfak.uni-bielefeld.de/pknotsrg/submission.html http://bibiserv.techfak.uni-bielefeld.de/pknotsrg/submission.html ADD cord-298078-uqrwq5qk 42 33 ) ) -RRB- cord-298078-uqrwq5qk 42 34 [ [ -LRB- cord-298078-uqrwq5qk 42 35 21 21 CD cord-298078-uqrwq5qk 42 36 , , , cord-298078-uqrwq5qk 42 37 22 22 CD cord-298078-uqrwq5qk 42 38 ] ] -RRB- cord-298078-uqrwq5qk 42 39 . . . cord-298078-uqrwq5qk 43 1 These these DT cord-298078-uqrwq5qk 43 2 predicted predict VBN cord-298078-uqrwq5qk 43 3 structure structure NN cord-298078-uqrwq5qk 43 4 are be VBP cord-298078-uqrwq5qk 43 5 largely largely RB cord-298078-uqrwq5qk 43 6 consistent consistent JJ cord-298078-uqrwq5qk 43 7 with with IN cord-298078-uqrwq5qk 43 8 the the DT cord-298078-uqrwq5qk 43 9 structure structure NN cord-298078-uqrwq5qk 43 10 previously previously RB cord-298078-uqrwq5qk 43 11 reported report VBD cord-298078-uqrwq5qk 43 12 for for IN cord-298078-uqrwq5qk 43 13 IBV IBV NNP cord-298078-uqrwq5qk 43 14 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 43 15 RNA RNA NNP cord-298078-uqrwq5qk 43 16 by by IN cord-298078-uqrwq5qk 43 17 Brierley Brierley NNP cord-298078-uqrwq5qk 43 18 et et NNP cord-298078-uqrwq5qk 43 19 al al NNP cord-298078-uqrwq5qk 43 20 . . . cord-298078-uqrwq5qk 44 1 ( ( -LRB- cord-298078-uqrwq5qk 44 2 1992 1992 CD cord-298078-uqrwq5qk 44 3 ) ) -RRB- cord-298078-uqrwq5qk 44 4 , , , cord-298078-uqrwq5qk 44 5 albeit albeit IN cord-298078-uqrwq5qk 44 6 with with IN cord-298078-uqrwq5qk 44 7 minor minor JJ cord-298078-uqrwq5qk 44 8 differences difference NNS cord-298078-uqrwq5qk 44 9 [ [ -LRB- cord-298078-uqrwq5qk 44 10 9 9 CD cord-298078-uqrwq5qk 44 11 ] ] -RRB- cord-298078-uqrwq5qk 44 12 . . . cord-298078-uqrwq5qk 45 1 To to TO cord-298078-uqrwq5qk 45 2 search search VB cord-298078-uqrwq5qk 45 3 for for IN cord-298078-uqrwq5qk 45 4 cellular cellular JJ cord-298078-uqrwq5qk 45 5 proteins protein NNS cord-298078-uqrwq5qk 45 6 that that WDT cord-298078-uqrwq5qk 45 7 directly directly RB cord-298078-uqrwq5qk 45 8 interacted interact VBD cord-298078-uqrwq5qk 45 9 with with IN cord-298078-uqrwq5qk 45 10 IBV IBV NNP cord-298078-uqrwq5qk 45 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 45 12 RNA RNA NNP cord-298078-uqrwq5qk 45 13 , , , cord-298078-uqrwq5qk 45 14 a a DT cord-298078-uqrwq5qk 45 15 RNA RNA NNP cord-298078-uqrwq5qk 45 16 pull pull VBP cord-298078-uqrwq5qk 45 17 down down RP cord-298078-uqrwq5qk 45 18 assay assay NN cord-298078-uqrwq5qk 45 19 was be VBD cord-298078-uqrwq5qk 45 20 performed perform VBN cord-298078-uqrwq5qk 45 21 in in IN cord-298078-uqrwq5qk 45 22 the the DT cord-298078-uqrwq5qk 45 23 presence presence NN cord-298078-uqrwq5qk 45 24 of of IN cord-298078-uqrwq5qk 45 25 cell cell NN cord-298078-uqrwq5qk 45 26 extracts extract NNS cord-298078-uqrwq5qk 45 27 ( ( -LRB- cord-298078-uqrwq5qk 45 28 Figure figure NN cord-298078-uqrwq5qk 45 29 2A 2a CD cord-298078-uqrwq5qk 45 30 ) ) -RRB- cord-298078-uqrwq5qk 45 31 . . . cord-298078-uqrwq5qk 46 1 Wild wild JJ cord-298078-uqrwq5qk 46 2 - - HYPH cord-298078-uqrwq5qk 46 3 type type NN cord-298078-uqrwq5qk 46 4 and and CC cord-298078-uqrwq5qk 46 5 mutant mutant JJ cord-298078-uqrwq5qk 46 6 IBV IBV NNP cord-298078-uqrwq5qk 46 7 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 46 8 RNAs rna NNS cord-298078-uqrwq5qk 46 9 were be VBD cord-298078-uqrwq5qk 46 10 end end NN cord-298078-uqrwq5qk 46 11 - - HYPH cord-298078-uqrwq5qk 46 12 labeled label VBN cord-298078-uqrwq5qk 46 13 with with IN cord-298078-uqrwq5qk 46 14 biotin biotin NN cord-298078-uqrwq5qk 46 15 to to TO cord-298078-uqrwq5qk 46 16 serve serve VB cord-298078-uqrwq5qk 46 17 as as IN cord-298078-uqrwq5qk 46 18 bait bait NN cord-298078-uqrwq5qk 46 19 for for IN cord-298078-uqrwq5qk 46 20 cellular cellular JJ cord-298078-uqrwq5qk 46 21 proteins protein NNS cord-298078-uqrwq5qk 46 22 that that WDT cord-298078-uqrwq5qk 46 23 might may MD cord-298078-uqrwq5qk 46 24 precipitate precipitate VB cord-298078-uqrwq5qk 46 25 with with IN cord-298078-uqrwq5qk 46 26 streptavidin streptavidin NN cord-298078-uqrwq5qk 46 27 - - HYPH cord-298078-uqrwq5qk 46 28 coated coat VBN cord-298078-uqrwq5qk 46 29 beads bead NNS cord-298078-uqrwq5qk 46 30 . . . cord-298078-uqrwq5qk 47 1 In in IN cord-298078-uqrwq5qk 47 2 addition addition NN cord-298078-uqrwq5qk 47 3 , , , cord-298078-uqrwq5qk 47 4 RNA RNA NNP cord-298078-uqrwq5qk 47 5 was be VBD cord-298078-uqrwq5qk 47 6 in in IN cord-298078-uqrwq5qk 47 7 vitro vitro FW cord-298078-uqrwq5qk 47 8 transcribed transcribe VBN cord-298078-uqrwq5qk 47 9 in in IN cord-298078-uqrwq5qk 47 10 the the DT cord-298078-uqrwq5qk 47 11 presence presence NN cord-298078-uqrwq5qk 47 12 of of IN cord-298078-uqrwq5qk 47 13 4-thio 4-thio CD cord-298078-uqrwq5qk 47 14 UTP UTP NNP cord-298078-uqrwq5qk 47 15 that that WDT cord-298078-uqrwq5qk 47 16 could could MD cord-298078-uqrwq5qk 47 17 serve serve VB cord-298078-uqrwq5qk 47 18 as as IN cord-298078-uqrwq5qk 47 19 crosslinker crosslinker NNP cord-298078-uqrwq5qk 47 20 for for IN cord-298078-uqrwq5qk 47 21 directly directly RB cord-298078-uqrwq5qk 47 22 interacting interact VBG cord-298078-uqrwq5qk 47 23 proteins protein NNS cord-298078-uqrwq5qk 47 24 . . . cord-298078-uqrwq5qk 48 1 IBV IBV NNP cord-298078-uqrwq5qk 48 2 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 48 3 RNA RNA NNP cord-298078-uqrwq5qk 48 4 was be VBD cord-298078-uqrwq5qk 48 5 incubated incubate VBN cord-298078-uqrwq5qk 48 6 with with IN cord-298078-uqrwq5qk 48 7 cell cell NN cord-298078-uqrwq5qk 48 8 extracts extract NNS cord-298078-uqrwq5qk 48 9 from from IN cord-298078-uqrwq5qk 48 10 human human JJ cord-298078-uqrwq5qk 48 11 lung lung NN cord-298078-uqrwq5qk 48 12 fibroblast fibroblast NN cord-298078-uqrwq5qk 48 13 IMR90 IMR90 NNP cord-298078-uqrwq5qk 48 14 , , , cord-298078-uqrwq5qk 48 15 which which WDT cord-298078-uqrwq5qk 48 16 is be VBZ cord-298078-uqrwq5qk 48 17 a a DT cord-298078-uqrwq5qk 48 18 natural natural JJ cord-298078-uqrwq5qk 48 19 host host NN cord-298078-uqrwq5qk 48 20 cell cell NN cord-298078-uqrwq5qk 48 21 for for IN cord-298078-uqrwq5qk 48 22 the the DT cord-298078-uqrwq5qk 48 23 infection infection NN cord-298078-uqrwq5qk 48 24 of of IN cord-298078-uqrwq5qk 48 25 IBV IBV NNP cord-298078-uqrwq5qk 48 26 . . . cord-298078-uqrwq5qk 49 1 The the DT cord-298078-uqrwq5qk 49 2 complex complex NN cord-298078-uqrwq5qk 49 3 was be VBD cord-298078-uqrwq5qk 49 4 irradiated irradiate VBN cord-298078-uqrwq5qk 49 5 with with IN cord-298078-uqrwq5qk 49 6 365 365 CD cord-298078-uqrwq5qk 49 7 nm nm NN cord-298078-uqrwq5qk 49 8 UV uv NN cord-298078-uqrwq5qk 49 9 light light NN cord-298078-uqrwq5qk 49 10 to to TO cord-298078-uqrwq5qk 49 11 directly directly RB cord-298078-uqrwq5qk 49 12 crosslink crosslink VB cord-298078-uqrwq5qk 49 13 interacting interact VBG cord-298078-uqrwq5qk 49 14 proteins protein NNS cord-298078-uqrwq5qk 49 15 to to IN cord-298078-uqrwq5qk 49 16 4-thio 4-thio CD cord-298078-uqrwq5qk 49 17 UTP UTP NNP cord-298078-uqrwq5qk 49 18 incorporated incorporate VBD cord-298078-uqrwq5qk 49 19 IBV IBV NNP cord-298078-uqrwq5qk 49 20 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 49 21 RNA RNA NNP cord-298078-uqrwq5qk 49 22 . . . cord-298078-uqrwq5qk 50 1 RNA rna NN cord-298078-uqrwq5qk 50 2 - - HYPH cord-298078-uqrwq5qk 50 3 protein protein NN cord-298078-uqrwq5qk 50 4 complexes complex NNS cord-298078-uqrwq5qk 50 5 were be VBD cord-298078-uqrwq5qk 50 6 precipitated precipitate VBN cord-298078-uqrwq5qk 50 7 with with IN cord-298078-uqrwq5qk 50 8 Streptavidin Streptavidin NNP cord-298078-uqrwq5qk 50 9 - - HYPH cord-298078-uqrwq5qk 50 10 coated coat VBN cord-298078-uqrwq5qk 50 11 magnetic magnetic JJ cord-298078-uqrwq5qk 50 12 beads bead NNS cord-298078-uqrwq5qk 50 13 to to TO cord-298078-uqrwq5qk 50 14 pulldown pulldown VB cord-298078-uqrwq5qk 50 15 any any DT cord-298078-uqrwq5qk 50 16 proteins protein NNS cord-298078-uqrwq5qk 50 17 bound bind VBN cord-298078-uqrwq5qk 50 18 to to IN cord-298078-uqrwq5qk 50 19 biotin biotin NN cord-298078-uqrwq5qk 50 20 - - HYPH cord-298078-uqrwq5qk 50 21 labeled label VBN cord-298078-uqrwq5qk 50 22 IBV IBV NNP cord-298078-uqrwq5qk 50 23 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 50 24 RNA RNA NNP cord-298078-uqrwq5qk 50 25 . . . cord-298078-uqrwq5qk 51 1 Proteins protein NNS cord-298078-uqrwq5qk 51 2 were be VBD cord-298078-uqrwq5qk 51 3 eluted elute VBN cord-298078-uqrwq5qk 51 4 from from IN cord-298078-uqrwq5qk 51 5 the the DT cord-298078-uqrwq5qk 51 6 beads bead NNS cord-298078-uqrwq5qk 51 7 , , , cord-298078-uqrwq5qk 51 8 fractionated fractionate VBN cord-298078-uqrwq5qk 51 9 by by IN cord-298078-uqrwq5qk 51 10 SDS SDS NNP cord-298078-uqrwq5qk 51 11 - - HYPH cord-298078-uqrwq5qk 51 12 PAGE PAGE NNP cord-298078-uqrwq5qk 51 13 and and CC cord-298078-uqrwq5qk 51 14 analyzed analyze VBN cord-298078-uqrwq5qk 51 15 by by IN cord-298078-uqrwq5qk 51 16 silver silver JJ cord-298078-uqrwq5qk 51 17 staining staining NN cord-298078-uqrwq5qk 51 18 . . . cord-298078-uqrwq5qk 52 1 Comparison comparison NN cord-298078-uqrwq5qk 52 2 of of IN cord-298078-uqrwq5qk 52 3 the the DT cord-298078-uqrwq5qk 52 4 bands band NNS cord-298078-uqrwq5qk 52 5 from from IN cord-298078-uqrwq5qk 52 6 wild wild JJ cord-298078-uqrwq5qk 52 7 - - HYPH cord-298078-uqrwq5qk 52 8 type type NN cord-298078-uqrwq5qk 52 9 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 52 10 RNA RNA NNP cord-298078-uqrwq5qk 52 11 bound bind VBN cord-298078-uqrwq5qk 52 12 to to IN cord-298078-uqrwq5qk 52 13 those those DT cord-298078-uqrwq5qk 52 14 from from IN cord-298078-uqrwq5qk 52 15 the the DT cord-298078-uqrwq5qk 52 16 mutant mutant NN cord-298078-uqrwq5qk 53 1 demonstrated demonstrate VBD cord-298078-uqrwq5qk 53 2 that that IN cord-298078-uqrwq5qk 53 3 most most JJS cord-298078-uqrwq5qk 53 4 of of IN cord-298078-uqrwq5qk 53 5 the the DT cord-298078-uqrwq5qk 53 6 bound bind VBN cord-298078-uqrwq5qk 53 7 proteins protein NNS cord-298078-uqrwq5qk 53 8 were be VBD cord-298078-uqrwq5qk 53 9 common common JJ cord-298078-uqrwq5qk 53 10 for for IN cord-298078-uqrwq5qk 53 11 two two CD cord-298078-uqrwq5qk 53 12 RNA RNA NNP cord-298078-uqrwq5qk 53 13 molecules molecule NNS cord-298078-uqrwq5qk 53 14 ( ( -LRB- cord-298078-uqrwq5qk 53 15 Figure figure NN cord-298078-uqrwq5qk 53 16 2A 2a CD cord-298078-uqrwq5qk 53 17 ) ) -RRB- cord-298078-uqrwq5qk 53 18 . . . cord-298078-uqrwq5qk 54 1 However however RB cord-298078-uqrwq5qk 54 2 , , , cord-298078-uqrwq5qk 54 3 a a DT cord-298078-uqrwq5qk 54 4 couple couple NN cord-298078-uqrwq5qk 54 5 of of IN cord-298078-uqrwq5qk 54 6 protein protein NN cord-298078-uqrwq5qk 54 7 bands band NNS cord-298078-uqrwq5qk 54 8 seemed seem VBD cord-298078-uqrwq5qk 54 9 to to TO cord-298078-uqrwq5qk 54 10 present present VB cord-298078-uqrwq5qk 54 11 more more RBR cord-298078-uqrwq5qk 54 12 in in IN cord-298078-uqrwq5qk 54 13 wild wild JJ cord-298078-uqrwq5qk 54 14 - - HYPH cord-298078-uqrwq5qk 54 15 type type NN cord-298078-uqrwq5qk 54 16 than than IN cord-298078-uqrwq5qk 54 17 mutant mutant JJ cord-298078-uqrwq5qk 54 18 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 54 19 RNA RNA NNP cord-298078-uqrwq5qk 54 20 , , , cord-298078-uqrwq5qk 54 21 so so RB cord-298078-uqrwq5qk 54 22 we -PRON- PRP cord-298078-uqrwq5qk 54 23 eluted elute VBD cord-298078-uqrwq5qk 54 24 those those DT cord-298078-uqrwq5qk 54 25 bands band NNS cord-298078-uqrwq5qk 54 26 from from IN cord-298078-uqrwq5qk 54 27 the the DT cord-298078-uqrwq5qk 54 28 gel gel NN cord-298078-uqrwq5qk 54 29 . . . cord-298078-uqrwq5qk 55 1 Identification identification NN cord-298078-uqrwq5qk 55 2 of of IN cord-298078-uqrwq5qk 55 3 the the DT cord-298078-uqrwq5qk 55 4 protein protein NN cord-298078-uqrwq5qk 55 5 by by IN cord-298078-uqrwq5qk 55 6 MALDI MALDI NNP cord-298078-uqrwq5qk 55 7 - - HYPH cord-298078-uqrwq5qk 55 8 TOF TOF NNP cord-298078-uqrwq5qk 55 9 - - HYPH cord-298078-uqrwq5qk 55 10 TOF TOF NNP cord-298078-uqrwq5qk 55 11 revealed reveal VBD cord-298078-uqrwq5qk 55 12 that that IN cord-298078-uqrwq5qk 55 13 one one CD cord-298078-uqrwq5qk 55 14 of of IN cord-298078-uqrwq5qk 55 15 such such JJ cord-298078-uqrwq5qk 55 16 proteins protein NNS cord-298078-uqrwq5qk 55 17 was be VBD cord-298078-uqrwq5qk 55 18 Annexin Annexin NNP cord-298078-uqrwq5qk 55 19 A2 A2 NNP cord-298078-uqrwq5qk 55 20 ( ( -LRB- cord-298078-uqrwq5qk 55 21 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 55 22 ) ) -RRB- cord-298078-uqrwq5qk 55 23 . . . cord-298078-uqrwq5qk 56 1 The the DT cord-298078-uqrwq5qk 56 2 previous previous JJ cord-298078-uqrwq5qk 56 3 experiment experiment NN cord-298078-uqrwq5qk 56 4 indicated indicate VBD cord-298078-uqrwq5qk 56 5 the the DT cord-298078-uqrwq5qk 56 6 possible possible JJ cord-298078-uqrwq5qk 56 7 interaction interaction NN cord-298078-uqrwq5qk 56 8 of of IN cord-298078-uqrwq5qk 56 9 ANXA2 anxa2 NN cord-298078-uqrwq5qk 56 10 to to IN cord-298078-uqrwq5qk 56 11 IBV IBV NNP cord-298078-uqrwq5qk 56 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 56 13 RNA RNA NNP cord-298078-uqrwq5qk 56 14 . . . cord-298078-uqrwq5qk 57 1 To to TO cord-298078-uqrwq5qk 57 2 confirm confirm VB cord-298078-uqrwq5qk 57 3 whether whether IN cord-298078-uqrwq5qk 57 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 57 5 could could MD cord-298078-uqrwq5qk 57 6 bind bind VB cord-298078-uqrwq5qk 57 7 wild wild JJ cord-298078-uqrwq5qk 57 8 - - HYPH cord-298078-uqrwq5qk 57 9 type type NN cord-298078-uqrwq5qk 57 10 IBV IBV NNP cord-298078-uqrwq5qk 57 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 57 12 RNA RNA NNP cord-298078-uqrwq5qk 57 13 but but CC cord-298078-uqrwq5qk 57 14 not not RB cord-298078-uqrwq5qk 57 15 mutant mutant JJ cord-298078-uqrwq5qk 57 16 IBV IBV NNP cord-298078-uqrwq5qk 57 17 RNA RNA NNP cord-298078-uqrwq5qk 57 18 , , , cord-298078-uqrwq5qk 57 19 we -PRON- PRP cord-298078-uqrwq5qk 57 20 performed perform VBD cord-298078-uqrwq5qk 57 21 UV uv NN cord-298078-uqrwq5qk 57 22 crosslinking crosslinking NN cord-298078-uqrwq5qk 57 23 assay assay NN cord-298078-uqrwq5qk 57 24 to to TO cord-298078-uqrwq5qk 57 25 show show VB cord-298078-uqrwq5qk 57 26 that that IN cord-298078-uqrwq5qk 57 27 radiolabeled radiolabele VBN cord-298078-uqrwq5qk 57 28 wild wild JJ cord-298078-uqrwq5qk 57 29 - - HYPH cord-298078-uqrwq5qk 57 30 type type NN cord-298078-uqrwq5qk 57 31 IBV IBV NNP cord-298078-uqrwq5qk 57 32 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 57 33 RNA RNA NNP cord-298078-uqrwq5qk 57 34 bound bind VBN cord-298078-uqrwq5qk 57 35 to to IN cord-298078-uqrwq5qk 57 36 ANXA2 anxa2 FW cord-298078-uqrwq5qk 57 37 protein protein NN cord-298078-uqrwq5qk 57 38 ( ( -LRB- cord-298078-uqrwq5qk 57 39 Figure figure NN cord-298078-uqrwq5qk 57 40 2B 2b CD cord-298078-uqrwq5qk 57 41 ) ) -RRB- cord-298078-uqrwq5qk 57 42 . . . cord-298078-uqrwq5qk 58 1 After after IN cord-298078-uqrwq5qk 58 2 RNase rnase NN cord-298078-uqrwq5qk 58 3 treatment treatment NN cord-298078-uqrwq5qk 58 4 on on IN cord-298078-uqrwq5qk 58 5 RNA rna NN cord-298078-uqrwq5qk 58 6 - - HYPH cord-298078-uqrwq5qk 58 7 protein protein NN cord-298078-uqrwq5qk 58 8 complex complex NN cord-298078-uqrwq5qk 58 9 , , , cord-298078-uqrwq5qk 58 10 radiolabeled radiolabele VBD cord-298078-uqrwq5qk 58 11 IBV IBV NNP cord-298078-uqrwq5qk 58 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 58 13 RNA RNA NNP cord-298078-uqrwq5qk 58 14 bound bind VBD cord-298078-uqrwq5qk 58 15 protein protein NN cord-298078-uqrwq5qk 58 16 bands band NNS cord-298078-uqrwq5qk 58 17 were be VBD cord-298078-uqrwq5qk 58 18 characterized characterize VBN cord-298078-uqrwq5qk 58 19 by by IN cord-298078-uqrwq5qk 58 20 Western western JJ cord-298078-uqrwq5qk 58 21 blot blot NN cord-298078-uqrwq5qk 58 22 analysis analysis NN cord-298078-uqrwq5qk 58 23 with with IN cord-298078-uqrwq5qk 58 24 anti anti JJ cord-298078-uqrwq5qk 58 25 - - JJ cord-298078-uqrwq5qk 58 26 ANXA2 anxa2 JJ cord-298078-uqrwq5qk 58 27 antibody antibody NN cord-298078-uqrwq5qk 58 28 . . . cord-298078-uqrwq5qk 59 1 Radiolabeled Radiolabeled NNP cord-298078-uqrwq5qk 59 2 RNA RNA NNP cord-298078-uqrwq5qk 59 3 was be VBD cord-298078-uqrwq5qk 59 4 found find VBN cord-298078-uqrwq5qk 59 5 near near IN cord-298078-uqrwq5qk 59 6 the the DT cord-298078-uqrwq5qk 59 7 size size NN cord-298078-uqrwq5qk 59 8 of of IN cord-298078-uqrwq5qk 59 9 the the DT cord-298078-uqrwq5qk 59 10 Cytosolic Cytosolic NNP cord-298078-uqrwq5qk 59 11 extracts extract NNS cord-298078-uqrwq5qk 59 12 were be VBD cord-298078-uqrwq5qk 59 13 prepared prepare VBN cord-298078-uqrwq5qk 59 14 from from IN cord-298078-uqrwq5qk 59 15 IMR90 IMR90 NNP cord-298078-uqrwq5qk 59 16 cells cell NNS cord-298078-uqrwq5qk 59 17 , , , cord-298078-uqrwq5qk 59 18 and and CC cord-298078-uqrwq5qk 59 19 the the DT cord-298078-uqrwq5qk 59 20 extracts extract NNS cord-298078-uqrwq5qk 59 21 were be VBD cord-298078-uqrwq5qk 59 22 incubated incubate VBN cord-298078-uqrwq5qk 59 23 with with IN cord-298078-uqrwq5qk 59 24 either either DT cord-298078-uqrwq5qk 59 25 wild wild JJ cord-298078-uqrwq5qk 59 26 - - HYPH cord-298078-uqrwq5qk 59 27 type type NN cord-298078-uqrwq5qk 59 28 or or CC cord-298078-uqrwq5qk 59 29 mutant mutant JJ cord-298078-uqrwq5qk 59 30 IBV IBV NNP cord-298078-uqrwq5qk 59 31 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 59 32 RNA RNA NNP cord-298078-uqrwq5qk 59 33 and and CC cord-298078-uqrwq5qk 59 34 precipitated precipitate VBN cord-298078-uqrwq5qk 59 35 with with IN cord-298078-uqrwq5qk 59 36 Streptavidin Streptavidin NNP cord-298078-uqrwq5qk 59 37 - - HYPH cord-298078-uqrwq5qk 59 38 coated coat VBN cord-298078-uqrwq5qk 59 39 magnetic magnetic JJ cord-298078-uqrwq5qk 59 40 beads bead NNS cord-298078-uqrwq5qk 59 41 . . . cord-298078-uqrwq5qk 60 1 Bound bind VBN cord-298078-uqrwq5qk 60 2 proteins protein NNS cord-298078-uqrwq5qk 60 3 ( ( -LRB- cord-298078-uqrwq5qk 60 4 pellet pellet NN cord-298078-uqrwq5qk 60 5 ) ) -RRB- cord-298078-uqrwq5qk 60 6 were be VBD cord-298078-uqrwq5qk 60 7 eluted elute VBN cord-298078-uqrwq5qk 60 8 from from IN cord-298078-uqrwq5qk 60 9 the the DT cord-298078-uqrwq5qk 60 10 beads bead NNS cord-298078-uqrwq5qk 60 11 , , , cord-298078-uqrwq5qk 60 12 fractionated fractionate VBN cord-298078-uqrwq5qk 60 13 using use VBG cord-298078-uqrwq5qk 60 14 10 10 CD cord-298078-uqrwq5qk 60 15 % % NN cord-298078-uqrwq5qk 60 16 SDS SDS NNP cord-298078-uqrwq5qk 60 17 - - HYPH cord-298078-uqrwq5qk 60 18 PAGE PAGE NNP cord-298078-uqrwq5qk 60 19 and and CC cord-298078-uqrwq5qk 60 20 stained stain VBN cord-298078-uqrwq5qk 60 21 with with IN cord-298078-uqrwq5qk 60 22 silver silver NN cord-298078-uqrwq5qk 60 23 . . . cord-298078-uqrwq5qk 61 1 Unbound unbound JJ cord-298078-uqrwq5qk 61 2 proteins protein NNS cord-298078-uqrwq5qk 61 3 ( ( -LRB- cord-298078-uqrwq5qk 61 4 sup sup NNP cord-298078-uqrwq5qk 61 5 ) ) -RRB- cord-298078-uqrwq5qk 61 6 were be VBD cord-298078-uqrwq5qk 61 7 also also RB cord-298078-uqrwq5qk 61 8 analyzed analyze VBN cord-298078-uqrwq5qk 61 9 as as IN cord-298078-uqrwq5qk 61 10 a a DT cord-298078-uqrwq5qk 61 11 control control NN cord-298078-uqrwq5qk 61 12 . . . cord-298078-uqrwq5qk 62 1 Input input NN cord-298078-uqrwq5qk 62 2 refers refer VBZ cord-298078-uqrwq5qk 62 3 to to IN cord-298078-uqrwq5qk 62 4 10 10 CD cord-298078-uqrwq5qk 62 5 % % NN cord-298078-uqrwq5qk 62 6 input input NN cord-298078-uqrwq5qk 62 7 of of IN cord-298078-uqrwq5qk 62 8 IMR90 IMR90 NNP cord-298078-uqrwq5qk 62 9 cell cell NN cord-298078-uqrwq5qk 62 10 extract extract NN cord-298078-uqrwq5qk 62 11 . . . cord-298078-uqrwq5qk 63 1 Bands band NNS cord-298078-uqrwq5qk 63 2 isolated isolate VBN cord-298078-uqrwq5qk 63 3 from from IN cord-298078-uqrwq5qk 63 4 the the DT cord-298078-uqrwq5qk 63 5 gel gel NN cord-298078-uqrwq5qk 63 6 are be VBP cord-298078-uqrwq5qk 63 7 also also RB cord-298078-uqrwq5qk 63 8 shown show VBN cord-298078-uqrwq5qk 63 9 on on IN cord-298078-uqrwq5qk 63 10 the the DT cord-298078-uqrwq5qk 63 11 right right NN cord-298078-uqrwq5qk 63 12 with with IN cord-298078-uqrwq5qk 63 13 higher high JJR cord-298078-uqrwq5qk 63 14 magnification magnification NN cord-298078-uqrwq5qk 63 15 . . . cord-298078-uqrwq5qk 64 1 MALDI MALDI NNP cord-298078-uqrwq5qk 64 2 - - HYPH cord-298078-uqrwq5qk 64 3 TOF TOF NNP cord-298078-uqrwq5qk 64 4 - - HYPH cord-298078-uqrwq5qk 64 5 TOF TOF NNP cord-298078-uqrwq5qk 64 6 analysis analysis NN cord-298078-uqrwq5qk 64 7 identified identify VBD cord-298078-uqrwq5qk 64 8 the the DT cord-298078-uqrwq5qk 64 9 wild wild JJ cord-298078-uqrwq5qk 64 10 - - HYPH cord-298078-uqrwq5qk 64 11 type type NN cord-298078-uqrwq5qk 64 12 bound bind VBN cord-298078-uqrwq5qk 64 13 protein protein NN cord-298078-uqrwq5qk 64 14 as as IN cord-298078-uqrwq5qk 64 15 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 64 16 , , , cord-298078-uqrwq5qk 64 17 indicated indicate VBN cord-298078-uqrwq5qk 64 18 with with IN cord-298078-uqrwq5qk 64 19 asterisk asterisk NN cord-298078-uqrwq5qk 64 20 . . . cord-298078-uqrwq5qk 65 1 ( ( -LRB- cord-298078-uqrwq5qk 65 2 B b NN cord-298078-uqrwq5qk 65 3 ) ) -RRB- cord-298078-uqrwq5qk 65 4 UV uv NN cord-298078-uqrwq5qk 65 5 crosslinking crosslinking NN cord-298078-uqrwq5qk 65 6 assay assay NN cord-298078-uqrwq5qk 65 7 ( ( -LRB- cord-298078-uqrwq5qk 65 8 left left RB cord-298078-uqrwq5qk 65 9 ) ) -RRB- cord-298078-uqrwq5qk 65 10 . . . cord-298078-uqrwq5qk 66 1 IMR90 IMR90 NNP cord-298078-uqrwq5qk 66 2 cell cell NN cord-298078-uqrwq5qk 66 3 extracts extract NNS cord-298078-uqrwq5qk 66 4 were be VBD cord-298078-uqrwq5qk 66 5 incubated incubate VBN cord-298078-uqrwq5qk 66 6 with with IN cord-298078-uqrwq5qk 66 7 the the DT cord-298078-uqrwq5qk 66 8 [ [ -LRB- cord-298078-uqrwq5qk 66 9 a-32 a-32 NNP cord-298078-uqrwq5qk 66 10 P P NNP cord-298078-uqrwq5qk 66 11 ] ] -RRB- cord-298078-uqrwq5qk 66 12 labeled label VBN cord-298078-uqrwq5qk 66 13 Wild wild JJ cord-298078-uqrwq5qk 66 14 - - HYPH cord-298078-uqrwq5qk 66 15 type type NN cord-298078-uqrwq5qk 66 16 ( ( -LRB- cord-298078-uqrwq5qk 66 17 WT WT NNP cord-298078-uqrwq5qk 66 18 ) ) -RRB- cord-298078-uqrwq5qk 66 19 or or CC cord-298078-uqrwq5qk 66 20 mutant mutant JJ cord-298078-uqrwq5qk 66 21 IBV IBV NNP cord-298078-uqrwq5qk 66 22 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 66 23 RNA RNA NNP cord-298078-uqrwq5qk 66 24 . . . cord-298078-uqrwq5qk 67 1 After after IN cord-298078-uqrwq5qk 67 2 crosslinking crosslinke VBG cord-298078-uqrwq5qk 67 3 with with IN cord-298078-uqrwq5qk 67 4 UV UV NNP cord-298078-uqrwq5qk 67 5 , , , cord-298078-uqrwq5qk 67 6 RNA rna NN cord-298078-uqrwq5qk 67 7 - - HYPH cord-298078-uqrwq5qk 67 8 protein protein NN cord-298078-uqrwq5qk 67 9 complexes complex NNS cord-298078-uqrwq5qk 67 10 were be VBD cord-298078-uqrwq5qk 67 11 untreated untreated JJ cord-298078-uqrwq5qk 67 12 ( ( -LRB- cord-298078-uqrwq5qk 67 13 - - : cord-298078-uqrwq5qk 67 14 ) ) -RRB- cord-298078-uqrwq5qk 67 15 or or CC cord-298078-uqrwq5qk 67 16 treated treat VBN cord-298078-uqrwq5qk 67 17 ( ( -LRB- cord-298078-uqrwq5qk 67 18 + + CC cord-298078-uqrwq5qk 67 19 ) ) -RRB- cord-298078-uqrwq5qk 67 20 with with IN cord-298078-uqrwq5qk 67 21 RNase RNase NNP cord-298078-uqrwq5qk 67 22 A A NNP cord-298078-uqrwq5qk 67 23 and and CC cord-298078-uqrwq5qk 67 24 fractionated fractionate VBN cord-298078-uqrwq5qk 67 25 in in IN cord-298078-uqrwq5qk 67 26 polyacrylamide polyacrylamide NN cord-298078-uqrwq5qk 67 27 gel gel NN cord-298078-uqrwq5qk 67 28 . . . cord-298078-uqrwq5qk 68 1 Two two CD cord-298078-uqrwq5qk 68 2 protein protein NN cord-298078-uqrwq5qk 68 3 - - HYPH cord-298078-uqrwq5qk 68 4 RNA rna NN cord-298078-uqrwq5qk 68 5 complex complex JJ cord-298078-uqrwq5qk 68 6 bands band NNS cord-298078-uqrwq5qk 68 7 from from IN cord-298078-uqrwq5qk 68 8 IMR90 IMR90 NNP cord-298078-uqrwq5qk 68 9 cell cell NN cord-298078-uqrwq5qk 68 10 extract extract NN cord-298078-uqrwq5qk 68 11 ( ( -LRB- cord-298078-uqrwq5qk 68 12 shown show VBN cord-298078-uqrwq5qk 68 13 with with IN cord-298078-uqrwq5qk 68 14 an an DT cord-298078-uqrwq5qk 68 15 arrow arrow NN cord-298078-uqrwq5qk 68 16 on on IN cord-298078-uqrwq5qk 68 17 the the DT cord-298078-uqrwq5qk 68 18 right right JJ cord-298078-uqrwq5qk 68 19 side side NN cord-298078-uqrwq5qk 68 20 of of IN cord-298078-uqrwq5qk 68 21 the the DT cord-298078-uqrwq5qk 68 22 gel gel NN cord-298078-uqrwq5qk 68 23 , , , cord-298078-uqrwq5qk 68 24 an an DT cord-298078-uqrwq5qk 68 25 asterisk asterisk NN cord-298078-uqrwq5qk 68 26 indicating indicate VBG cord-298078-uqrwq5qk 68 27 non non JJ cord-298078-uqrwq5qk 68 28 - - JJ cord-298078-uqrwq5qk 68 29 specific specific JJ cord-298078-uqrwq5qk 68 30 band band NN cord-298078-uqrwq5qk 68 31 ) ) -RRB- cord-298078-uqrwq5qk 68 32 were be VBD cord-298078-uqrwq5qk 68 33 shown show VBN cord-298078-uqrwq5qk 68 34 to to TO cord-298078-uqrwq5qk 68 35 be be VB cord-298078-uqrwq5qk 68 36 specific specific JJ cord-298078-uqrwq5qk 68 37 to to IN cord-298078-uqrwq5qk 68 38 Wild wild JJ cord-298078-uqrwq5qk 68 39 - - HYPH cord-298078-uqrwq5qk 68 40 type type NN cord-298078-uqrwq5qk 68 41 ( ( -LRB- cord-298078-uqrwq5qk 68 42 WT WT NNP cord-298078-uqrwq5qk 68 43 ) ) -RRB- cord-298078-uqrwq5qk 68 44 IBV IBV NNP cord-298078-uqrwq5qk 68 45 RNA RNA NNP cord-298078-uqrwq5qk 68 46 . . . cord-298078-uqrwq5qk 69 1 Competition competition NN cord-298078-uqrwq5qk 69 2 assay assay NN cord-298078-uqrwq5qk 69 3 ( ( -LRB- cord-298078-uqrwq5qk 69 4 middle middle JJ cord-298078-uqrwq5qk 69 5 ) ) -RRB- cord-298078-uqrwq5qk 69 6 . . . cord-298078-uqrwq5qk 70 1 Cell cell NN cord-298078-uqrwq5qk 70 2 extracts extract NNS cord-298078-uqrwq5qk 70 3 were be VBD cord-298078-uqrwq5qk 70 4 incubated incubate VBN cord-298078-uqrwq5qk 70 5 with with IN cord-298078-uqrwq5qk 70 6 the the DT cord-298078-uqrwq5qk 70 7 [ [ -LRB- cord-298078-uqrwq5qk 70 8 a-32 a-32 NNP cord-298078-uqrwq5qk 70 9 P P NNP cord-298078-uqrwq5qk 70 10 ] ] -RRB- cord-298078-uqrwq5qk 70 11 labeled label VBN cord-298078-uqrwq5qk 70 12 Wild wild JJ cord-298078-uqrwq5qk 70 13 - - HYPH cord-298078-uqrwq5qk 70 14 type type NN cord-298078-uqrwq5qk 70 15 ( ( -LRB- cord-298078-uqrwq5qk 70 16 WT WT NNP cord-298078-uqrwq5qk 70 17 ) ) -RRB- cord-298078-uqrwq5qk 70 18 or or CC cord-298078-uqrwq5qk 70 19 mutant mutant JJ cord-298078-uqrwq5qk 70 20 ( ( -LRB- cord-298078-uqrwq5qk 70 21 MT MT NNP cord-298078-uqrwq5qk 70 22 ) ) -RRB- cord-298078-uqrwq5qk 70 23 IBV IBV NNP cord-298078-uqrwq5qk 70 24 RNA RNA NNP cord-298078-uqrwq5qk 70 25 and and CC cord-298078-uqrwq5qk 70 26 non non JJ cord-298078-uqrwq5qk 70 27 - - JJ cord-298078-uqrwq5qk 70 28 labeled labeled JJ cord-298078-uqrwq5qk 70 29 competitor competitor NN cord-298078-uqrwq5qk 70 30 ( ( -LRB- cord-298078-uqrwq5qk 70 31 16 16 CD cord-298078-uqrwq5qk 70 32 , , , cord-298078-uqrwq5qk 70 33 106 106 CD cord-298078-uqrwq5qk 70 34 and and CC cord-298078-uqrwq5qk 70 35 1006 1006 CD cord-298078-uqrwq5qk 70 36 , , , cord-298078-uqrwq5qk 70 37 respectively respectively RB cord-298078-uqrwq5qk 70 38 ) ) -RRB- cord-298078-uqrwq5qk 70 39 . . . cord-298078-uqrwq5qk 71 1 Western western JJ cord-298078-uqrwq5qk 71 2 blot blot NN cord-298078-uqrwq5qk 71 3 analysis analysis NN cord-298078-uqrwq5qk 71 4 of of IN cord-298078-uqrwq5qk 71 5 UV uv NN cord-298078-uqrwq5qk 71 6 - - HYPH cord-298078-uqrwq5qk 71 7 crosslinked crosslinke VBN cord-298078-uqrwq5qk 71 8 RNA RNA NNP cord-298078-uqrwq5qk 71 9 - - HYPH cord-298078-uqrwq5qk 71 10 protein protein NN cord-298078-uqrwq5qk 71 11 complexes complex NNS cord-298078-uqrwq5qk 71 12 ( ( -LRB- cord-298078-uqrwq5qk 71 13 right right RB cord-298078-uqrwq5qk 71 14 ) ) -RRB- cord-298078-uqrwq5qk 71 15 . . . cord-298078-uqrwq5qk 72 1 The the DT cord-298078-uqrwq5qk 72 2 same same JJ cord-298078-uqrwq5qk 72 3 gel gel NN cord-298078-uqrwq5qk 72 4 as as IN cord-298078-uqrwq5qk 72 5 shown show VBN cord-298078-uqrwq5qk 72 6 on on IN cord-298078-uqrwq5qk 72 7 the the DT cord-298078-uqrwq5qk 72 8 left left NN cord-298078-uqrwq5qk 72 9 was be VBD cord-298078-uqrwq5qk 72 10 blotted blot VBN cord-298078-uqrwq5qk 72 11 and and CC cord-298078-uqrwq5qk 72 12 incubated incubate VBN cord-298078-uqrwq5qk 72 13 with with IN cord-298078-uqrwq5qk 72 14 anti anti JJ cord-298078-uqrwq5qk 72 15 - - JJ cord-298078-uqrwq5qk 72 16 ANXA2 anxa2 JJ cord-298078-uqrwq5qk 72 17 antibody antibody NN cord-298078-uqrwq5qk 72 18 . . . cord-298078-uqrwq5qk 73 1 All all DT cord-298078-uqrwq5qk 73 2 data datum NNS cord-298078-uqrwq5qk 73 3 were be VBD cord-298078-uqrwq5qk 73 4 generated generate VBN cord-298078-uqrwq5qk 73 5 by by IN cord-298078-uqrwq5qk 73 6 the the DT cord-298078-uqrwq5qk 73 7 results result NNS cord-298078-uqrwq5qk 73 8 from from IN cord-298078-uqrwq5qk 73 9 at at RB cord-298078-uqrwq5qk 73 10 least least RBS cord-298078-uqrwq5qk 73 11 three three CD cord-298078-uqrwq5qk 73 12 independent independent JJ cord-298078-uqrwq5qk 73 13 experiments experiment NNS cord-298078-uqrwq5qk 73 14 . . . cord-298078-uqrwq5qk 74 1 doi:10.1371/ doi:10.1371/ NNP cord-298078-uqrwq5qk 75 1 journal.pone.0024067.g002 journal.pone.0024067.g002 NNP cord-298078-uqrwq5qk 75 2 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 75 3 protein protein NN cord-298078-uqrwq5qk 75 4 , , , cord-298078-uqrwq5qk 75 5 which which WDT cord-298078-uqrwq5qk 75 6 suggests suggest VBZ cord-298078-uqrwq5qk 75 7 an an DT cord-298078-uqrwq5qk 75 8 interaction interaction NN cord-298078-uqrwq5qk 75 9 between between IN cord-298078-uqrwq5qk 75 10 radiolabeled radiolabele VBN cord-298078-uqrwq5qk 75 11 IBV IBV NNP cord-298078-uqrwq5qk 75 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 75 13 RNA RNA NNP cord-298078-uqrwq5qk 75 14 and and CC cord-298078-uqrwq5qk 75 15 cellular cellular JJ cord-298078-uqrwq5qk 75 16 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 75 17 protein protein NN cord-298078-uqrwq5qk 75 18 . . . cord-298078-uqrwq5qk 76 1 Significantly significantly RB cord-298078-uqrwq5qk 76 2 , , , cord-298078-uqrwq5qk 76 3 this this DT cord-298078-uqrwq5qk 76 4 band band NN cord-298078-uqrwq5qk 76 5 specifically specifically RB cord-298078-uqrwq5qk 76 6 competed compete VBD cord-298078-uqrwq5qk 76 7 with with IN cord-298078-uqrwq5qk 76 8 wild wild JJ cord-298078-uqrwq5qk 76 9 - - HYPH cord-298078-uqrwq5qk 76 10 type type NN cord-298078-uqrwq5qk 76 11 IBV IBV NNP cord-298078-uqrwq5qk 76 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 76 13 RNA RNA NNP cord-298078-uqrwq5qk 76 14 but but CC cord-298078-uqrwq5qk 76 15 not not RB cord-298078-uqrwq5qk 76 16 with with IN cord-298078-uqrwq5qk 76 17 mutant mutant JJ cord-298078-uqrwq5qk 76 18 IBV IBV NNP cord-298078-uqrwq5qk 76 19 RNA RNA NNP cord-298078-uqrwq5qk 76 20 , , , cord-298078-uqrwq5qk 76 21 also also RB cord-298078-uqrwq5qk 76 22 supporting support VBG cord-298078-uqrwq5qk 76 23 the the DT cord-298078-uqrwq5qk 76 24 binding bind VBG cord-298078-uqrwq5qk 76 25 specificity specificity NN cord-298078-uqrwq5qk 76 26 of of IN cord-298078-uqrwq5qk 76 27 ANXA2 anxa2 NN cord-298078-uqrwq5qk 76 28 to to IN cord-298078-uqrwq5qk 76 29 wild wild JJ cord-298078-uqrwq5qk 76 30 - - HYPH cord-298078-uqrwq5qk 76 31 type type NN cord-298078-uqrwq5qk 76 32 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 76 33 RNA RNA NNP cord-298078-uqrwq5qk 76 34 ( ( -LRB- cord-298078-uqrwq5qk 76 35 Figure Figure NNP cord-298078-uqrwq5qk 76 36 2B 2b CD cord-298078-uqrwq5qk 76 37 ) ) -RRB- cord-298078-uqrwq5qk 76 38 . . . cord-298078-uqrwq5qk 77 1 Identity identity NN cord-298078-uqrwq5qk 77 2 of of IN cord-298078-uqrwq5qk 77 3 the the DT cord-298078-uqrwq5qk 77 4 RNA RNA NNP cord-298078-uqrwq5qk 77 5 bound bind VBN cord-298078-uqrwq5qk 77 6 protein protein NN cord-298078-uqrwq5qk 77 7 was be VBD cord-298078-uqrwq5qk 77 8 also also RB cord-298078-uqrwq5qk 77 9 confirmed confirm VBN cord-298078-uqrwq5qk 77 10 by by IN cord-298078-uqrwq5qk 77 11 Western western JJ cord-298078-uqrwq5qk 77 12 blot blot NN cord-298078-uqrwq5qk 77 13 analysis analysis NN cord-298078-uqrwq5qk 77 14 of of IN cord-298078-uqrwq5qk 77 15 the the DT cord-298078-uqrwq5qk 77 16 same same JJ cord-298078-uqrwq5qk 77 17 gel gel NN cord-298078-uqrwq5qk 77 18 ( ( -LRB- cord-298078-uqrwq5qk 77 19 Figure figure NN cord-298078-uqrwq5qk 77 20 2B 2b CD cord-298078-uqrwq5qk 77 21 ) ) -RRB- cord-298078-uqrwq5qk 77 22 . . . cord-298078-uqrwq5qk 78 1 These these DT cord-298078-uqrwq5qk 78 2 data datum NNS cord-298078-uqrwq5qk 78 3 clearly clearly RB cord-298078-uqrwq5qk 78 4 demonstrated demonstrate VBD cord-298078-uqrwq5qk 78 5 binding binding NN cord-298078-uqrwq5qk 78 6 of of IN cord-298078-uqrwq5qk 78 7 ANXA2 anxa2 NN cord-298078-uqrwq5qk 78 8 to to IN cord-298078-uqrwq5qk 78 9 the the DT cord-298078-uqrwq5qk 78 10 frameshifting frameshifte VBG cord-298078-uqrwq5qk 78 11 RNA RNA NNP cord-298078-uqrwq5qk 78 12 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 78 13 in in IN cord-298078-uqrwq5qk 78 14 the the DT cord-298078-uqrwq5qk 78 15 IBV IBV NNP cord-298078-uqrwq5qk 78 16 genome genome NN cord-298078-uqrwq5qk 78 17 . . . cord-298078-uqrwq5qk 79 1 Next next RB cord-298078-uqrwq5qk 79 2 , , , cord-298078-uqrwq5qk 79 3 we -PRON- PRP cord-298078-uqrwq5qk 79 4 tested test VBD cord-298078-uqrwq5qk 79 5 whether whether IN cord-298078-uqrwq5qk 79 6 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 79 7 could could MD cord-298078-uqrwq5qk 79 8 bind bind VB cord-298078-uqrwq5qk 79 9 directly directly RB cord-298078-uqrwq5qk 79 10 to to IN cord-298078-uqrwq5qk 79 11 IBV IBV NNP cord-298078-uqrwq5qk 79 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 79 13 RNA RNA NNP cord-298078-uqrwq5qk 79 14 . . . cord-298078-uqrwq5qk 80 1 To to TO cord-298078-uqrwq5qk 80 2 explore explore VB cord-298078-uqrwq5qk 80 3 this this DT cord-298078-uqrwq5qk 80 4 , , , cord-298078-uqrwq5qk 80 5 GST GST NNP cord-298078-uqrwq5qk 80 6 - - HYPH cord-298078-uqrwq5qk 80 7 fused fuse VBN cord-298078-uqrwq5qk 80 8 recombinant recombinant JJ cord-298078-uqrwq5qk 80 9 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 80 10 protein protein NN cord-298078-uqrwq5qk 80 11 was be VBD cord-298078-uqrwq5qk 80 12 purified purify VBN cord-298078-uqrwq5qk 80 13 and and CC cord-298078-uqrwq5qk 80 14 used use VBN cord-298078-uqrwq5qk 80 15 for for IN cord-298078-uqrwq5qk 80 16 GST GST NNP cord-298078-uqrwq5qk 80 17 pull pull VB cord-298078-uqrwq5qk 80 18 - - HYPH cord-298078-uqrwq5qk 80 19 down down RP cord-298078-uqrwq5qk 80 20 assay assay NN cord-298078-uqrwq5qk 80 21 . . . cord-298078-uqrwq5qk 81 1 More More JJR cord-298078-uqrwq5qk 81 2 radioactivity radioactivity NN cord-298078-uqrwq5qk 81 3 was be VBD cord-298078-uqrwq5qk 81 4 recovered recover VBN cord-298078-uqrwq5qk 81 5 after after IN cord-298078-uqrwq5qk 81 6 precipitating precipitate VBG cord-298078-uqrwq5qk 81 7 with with IN cord-298078-uqrwq5qk 81 8 GST GST NNP cord-298078-uqrwq5qk 81 9 - - HYPH cord-298078-uqrwq5qk 81 10 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 81 11 in in IN cord-298078-uqrwq5qk 81 12 comparison comparison NN cord-298078-uqrwq5qk 81 13 to to IN cord-298078-uqrwq5qk 81 14 GST GST NNP cord-298078-uqrwq5qk 81 15 , , , cord-298078-uqrwq5qk 81 16 suggesting suggest VBG cord-298078-uqrwq5qk 81 17 that that IN cord-298078-uqrwq5qk 81 18 recombinant recombinant JJ cord-298078-uqrwq5qk 81 19 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 81 20 could could MD cord-298078-uqrwq5qk 81 21 associate associate VB cord-298078-uqrwq5qk 81 22 with with IN cord-298078-uqrwq5qk 81 23 IBV IBV NNP cord-298078-uqrwq5qk 81 24 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 81 25 RNA RNA NNP cord-298078-uqrwq5qk 81 26 . . . cord-298078-uqrwq5qk 82 1 Moreover moreover RB cord-298078-uqrwq5qk 82 2 , , , cord-298078-uqrwq5qk 82 3 wild wild JJ cord-298078-uqrwq5qk 82 4 - - HYPH cord-298078-uqrwq5qk 82 5 type type NN cord-298078-uqrwq5qk 82 6 IBV IBV NNP cord-298078-uqrwq5qk 82 7 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 82 8 RNA RNA NNP cord-298078-uqrwq5qk 82 9 tended tend VBD cord-298078-uqrwq5qk 82 10 to to TO cord-298078-uqrwq5qk 82 11 bind bind VB cord-298078-uqrwq5qk 82 12 more more JJR cord-298078-uqrwq5qk 82 13 than than IN cord-298078-uqrwq5qk 82 14 mutant mutant JJ cord-298078-uqrwq5qk 82 15 IBV IBV NNP cord-298078-uqrwq5qk 82 16 RNA RNA NNP cord-298078-uqrwq5qk 82 17 did do VBD cord-298078-uqrwq5qk 82 18 ( ( -LRB- cord-298078-uqrwq5qk 82 19 Figure Figure NNP cord-298078-uqrwq5qk 82 20 3A 3a CD cord-298078-uqrwq5qk 82 21 ) ) -RRB- cord-298078-uqrwq5qk 82 22 . . . cord-298078-uqrwq5qk 83 1 We -PRON- PRP cord-298078-uqrwq5qk 83 2 tested test VBD cord-298078-uqrwq5qk 83 3 whether whether IN cord-298078-uqrwq5qk 83 4 such such JJ cord-298078-uqrwq5qk 83 5 binding binding NN cord-298078-uqrwq5qk 83 6 was be VBD cord-298078-uqrwq5qk 83 7 dependent dependent JJ cord-298078-uqrwq5qk 83 8 on on IN cord-298078-uqrwq5qk 83 9 the the DT cord-298078-uqrwq5qk 83 10 presence presence NN cord-298078-uqrwq5qk 83 11 of of IN cord-298078-uqrwq5qk 83 12 calcium calcium NN cord-298078-uqrwq5qk 83 13 , , , cord-298078-uqrwq5qk 83 14 given give VBN cord-298078-uqrwq5qk 83 15 that that IN cord-298078-uqrwq5qk 83 16 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 83 17 is be VBZ cord-298078-uqrwq5qk 83 18 a a DT cord-298078-uqrwq5qk 83 19 calcium calcium NN cord-298078-uqrwq5qk 83 20 binding bind VBG cord-298078-uqrwq5qk 83 21 protein protein NN cord-298078-uqrwq5qk 83 22 . . . cord-298078-uqrwq5qk 84 1 However however RB cord-298078-uqrwq5qk 84 2 , , , cord-298078-uqrwq5qk 84 3 calcium calcium NN cord-298078-uqrwq5qk 84 4 did do VBD cord-298078-uqrwq5qk 84 5 not not RB cord-298078-uqrwq5qk 84 6 cause cause VB cord-298078-uqrwq5qk 84 7 any any DT cord-298078-uqrwq5qk 84 8 change change NN cord-298078-uqrwq5qk 84 9 in in IN cord-298078-uqrwq5qk 84 10 the the DT cord-298078-uqrwq5qk 84 11 binding binding NN cord-298078-uqrwq5qk 84 12 of of IN cord-298078-uqrwq5qk 84 13 ANXA2 anxa2 NN cord-298078-uqrwq5qk 84 14 to to IN cord-298078-uqrwq5qk 84 15 IBV IBV NNP cord-298078-uqrwq5qk 84 16 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 84 17 RNA RNA NNP cord-298078-uqrwq5qk 84 18 ( ( -LRB- cord-298078-uqrwq5qk 84 19 data datum NNS cord-298078-uqrwq5qk 84 20 not not RB cord-298078-uqrwq5qk 84 21 shown show VBN cord-298078-uqrwq5qk 84 22 ) ) -RRB- cord-298078-uqrwq5qk 84 23 . . . cord-298078-uqrwq5qk 85 1 We -PRON- PRP cord-298078-uqrwq5qk 85 2 performed perform VBD cord-298078-uqrwq5qk 85 3 a a DT cord-298078-uqrwq5qk 85 4 RNA RNA NNP cord-298078-uqrwq5qk 85 5 - - HYPH cord-298078-uqrwq5qk 85 6 EMSA EMSA NNS cord-298078-uqrwq5qk 85 7 to to TO cord-298078-uqrwq5qk 85 8 analyze analyze VB cord-298078-uqrwq5qk 85 9 the the DT cord-298078-uqrwq5qk 85 10 ANXA2 anxa2 NN cord-298078-uqrwq5qk 85 11 binding binding NN cord-298078-uqrwq5qk 85 12 pattern pattern NN cord-298078-uqrwq5qk 85 13 ( ( -LRB- cord-298078-uqrwq5qk 85 14 Figure figure NN cord-298078-uqrwq5qk 85 15 3B 3b CD cord-298078-uqrwq5qk 85 16 ) ) -RRB- cord-298078-uqrwq5qk 85 17 . . . cord-298078-uqrwq5qk 86 1 RNA rna NN cord-298078-uqrwq5qk 86 2 - - HYPH cord-298078-uqrwq5qk 86 3 protein protein NN cord-298078-uqrwq5qk 86 4 complexes complex NNS cord-298078-uqrwq5qk 86 5 were be VBD cord-298078-uqrwq5qk 86 6 resolved resolve VBN cord-298078-uqrwq5qk 86 7 using use VBG cord-298078-uqrwq5qk 86 8 native native JJ cord-298078-uqrwq5qk 86 9 polyacrylamide polyacrylamide NN cord-298078-uqrwq5qk 86 10 gels gel NNS cord-298078-uqrwq5qk 86 11 . . . cord-298078-uqrwq5qk 87 1 Only only RB cord-298078-uqrwq5qk 87 2 a a DT cord-298078-uqrwq5qk 87 3 single single JJ cord-298078-uqrwq5qk 87 4 shifted shifted JJ cord-298078-uqrwq5qk 87 5 band band NN cord-298078-uqrwq5qk 87 6 was be VBD cord-298078-uqrwq5qk 87 7 generated generate VBN cord-298078-uqrwq5qk 87 8 in in IN cord-298078-uqrwq5qk 87 9 the the DT cord-298078-uqrwq5qk 87 10 presence presence NN cord-298078-uqrwq5qk 87 11 of of IN cord-298078-uqrwq5qk 87 12 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 87 13 protein protein NN cord-298078-uqrwq5qk 87 14 but but CC cord-298078-uqrwq5qk 87 15 not not RB cord-298078-uqrwq5qk 87 16 with with IN cord-298078-uqrwq5qk 87 17 the the DT cord-298078-uqrwq5qk 87 18 same same JJ cord-298078-uqrwq5qk 87 19 amount amount NN cord-298078-uqrwq5qk 87 20 of of IN cord-298078-uqrwq5qk 87 21 the the DT cord-298078-uqrwq5qk 87 22 GST GST NNP cord-298078-uqrwq5qk 87 23 protein protein NN cord-298078-uqrwq5qk 87 24 ( ( -LRB- cord-298078-uqrwq5qk 87 25 Figure figure NN cord-298078-uqrwq5qk 87 26 3B 3b CD cord-298078-uqrwq5qk 87 27 ) ) -RRB- cord-298078-uqrwq5qk 87 28 . . . cord-298078-uqrwq5qk 88 1 We -PRON- PRP cord-298078-uqrwq5qk 88 2 also also RB cord-298078-uqrwq5qk 88 3 confirmed confirm VBD cord-298078-uqrwq5qk 88 4 whether whether IN cord-298078-uqrwq5qk 88 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 88 6 bound bind VBN cord-298078-uqrwq5qk 88 7 to to IN cord-298078-uqrwq5qk 88 8 wild wild JJ cord-298078-uqrwq5qk 88 9 - - HYPH cord-298078-uqrwq5qk 88 10 type type NN cord-298078-uqrwq5qk 88 11 IBV IBV NNP cord-298078-uqrwq5qk 88 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 88 13 RNA RNA NNP cord-298078-uqrwq5qk 88 14 in in IN cord-298078-uqrwq5qk 88 15 the the DT cord-298078-uqrwq5qk 88 16 cells cell NNS cord-298078-uqrwq5qk 88 17 . . . cord-298078-uqrwq5qk 89 1 Through through IN cord-298078-uqrwq5qk 89 2 the the DT cord-298078-uqrwq5qk 89 3 RNA RNA NNP cord-298078-uqrwq5qk 89 4 - - HYPH cord-298078-uqrwq5qk 89 5 immunoprecipitation immunoprecipitation NN cord-298078-uqrwq5qk 89 6 assay assay NN cord-298078-uqrwq5qk 89 7 , , , cord-298078-uqrwq5qk 89 8 we -PRON- PRP cord-298078-uqrwq5qk 89 9 showed show VBD cord-298078-uqrwq5qk 89 10 that that IN cord-298078-uqrwq5qk 89 11 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 89 12 specifically specifically RB cord-298078-uqrwq5qk 89 13 interacted interact VBN cord-298078-uqrwq5qk 89 14 with with IN cord-298078-uqrwq5qk 89 15 wild wild JJ cord-298078-uqrwq5qk 89 16 - - HYPH cord-298078-uqrwq5qk 89 17 type type NN cord-298078-uqrwq5qk 89 18 IBV IBV NNP cord-298078-uqrwq5qk 89 19 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 89 20 RNA RNA NNP cord-298078-uqrwq5qk 89 21 but but CC cord-298078-uqrwq5qk 89 22 not not RB cord-298078-uqrwq5qk 89 23 with with IN cord-298078-uqrwq5qk 89 24 mutant mutant JJ cord-298078-uqrwq5qk 89 25 IBV IBV NNP cord-298078-uqrwq5qk 89 26 RNA RNA NNP cord-298078-uqrwq5qk 89 27 in in IN cord-298078-uqrwq5qk 89 28 LNCaP lncap NN cord-298078-uqrwq5qk 89 29 and and CC cord-298078-uqrwq5qk 89 30 HEK293 HEK293 NNP cord-298078-uqrwq5qk 89 31 T t NN cord-298078-uqrwq5qk 89 32 cells cell NNS cord-298078-uqrwq5qk 89 33 ( ( -LRB- cord-298078-uqrwq5qk 89 34 Figure Figure NNP cord-298078-uqrwq5qk 89 35 3C 3C NNP cord-298078-uqrwq5qk 89 36 and and CC cord-298078-uqrwq5qk 89 37 3D 3D NNP cord-298078-uqrwq5qk 89 38 ) ) -RRB- cord-298078-uqrwq5qk 89 39 . . . cord-298078-uqrwq5qk 90 1 These these DT cord-298078-uqrwq5qk 90 2 data datum NNS cord-298078-uqrwq5qk 90 3 clearly clearly RB cord-298078-uqrwq5qk 90 4 demonstrated demonstrate VBD cord-298078-uqrwq5qk 90 5 the the DT cord-298078-uqrwq5qk 90 6 specific specific JJ cord-298078-uqrwq5qk 90 7 binding binding NN cord-298078-uqrwq5qk 90 8 of of IN cord-298078-uqrwq5qk 90 9 ANXA2 anxa2 NN cord-298078-uqrwq5qk 90 10 to to IN cord-298078-uqrwq5qk 90 11 IBV IBV NNP cord-298078-uqrwq5qk 90 12 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 90 13 RNA RNA NNP cord-298078-uqrwq5qk 90 14 . . . cord-298078-uqrwq5qk 91 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 91 2 regulates regulate VBZ cord-298078-uqrwq5qk 91 3 ribosomal ribosomal JJ cord-298078-uqrwq5qk 91 4 frameshifting frameshifting NN cord-298078-uqrwq5qk 91 5 of of IN cord-298078-uqrwq5qk 91 6 IBV IBV NNP cord-298078-uqrwq5qk 91 7 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 91 8 RNA RNA NNP cord-298078-uqrwq5qk 92 1 Since since IN cord-298078-uqrwq5qk 92 2 we -PRON- PRP cord-298078-uqrwq5qk 92 3 found find VBD cord-298078-uqrwq5qk 92 4 that that IN cord-298078-uqrwq5qk 92 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 92 6 bound bind VBN cord-298078-uqrwq5qk 92 7 IBV IBV NNP cord-298078-uqrwq5qk 92 8 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 92 9 RNA RNA NNP cord-298078-uqrwq5qk 92 10 , , , cord-298078-uqrwq5qk 92 11 we -PRON- PRP cord-298078-uqrwq5qk 92 12 next next RB cord-298078-uqrwq5qk 92 13 tested test VBD cord-298078-uqrwq5qk 92 14 whether whether IN cord-298078-uqrwq5qk 92 15 it -PRON- PRP cord-298078-uqrwq5qk 92 16 could could MD cord-298078-uqrwq5qk 92 17 control control VB cord-298078-uqrwq5qk 92 18 the the DT cord-298078-uqrwq5qk 92 19 efficiency efficiency NN cord-298078-uqrwq5qk 92 20 of of IN cord-298078-uqrwq5qk 92 21 ribosomal ribosomal JJ cord-298078-uqrwq5qk 92 22 frameshifting frameshifte VBG cord-298078-uqrwq5qk 92 23 on on IN cord-298078-uqrwq5qk 92 24 IBV IBV NNP cord-298078-uqrwq5qk 92 25 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 92 26 RNA RNA NNP cord-298078-uqrwq5qk 92 27 in in IN cord-298078-uqrwq5qk 92 28 the the DT cord-298078-uqrwq5qk 92 29 cells cell NNS cord-298078-uqrwq5qk 92 30 . . . cord-298078-uqrwq5qk 93 1 We -PRON- PRP cord-298078-uqrwq5qk 93 2 constructed construct VBD cord-298078-uqrwq5qk 93 3 dual dual JJ cord-298078-uqrwq5qk 93 4 luciferase luciferase NN cord-298078-uqrwq5qk 93 5 reporters reporter NNS cord-298078-uqrwq5qk 93 6 controlled control VBN cord-298078-uqrwq5qk 93 7 by by IN cord-298078-uqrwq5qk 93 8 IBV IBV NNP cord-298078-uqrwq5qk 93 9 frameshifting frameshifte VBG cord-298078-uqrwq5qk 93 10 sequences sequence NNS cord-298078-uqrwq5qk 93 11 with with IN cord-298078-uqrwq5qk 93 12 or or CC cord-298078-uqrwq5qk 93 13 without without IN cord-298078-uqrwq5qk 93 14 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 93 15 RNA RNA NNP cord-298078-uqrwq5qk 93 16 ( ( -LRB- cord-298078-uqrwq5qk 93 17 Figure figure NN cord-298078-uqrwq5qk 93 18 4A 4a CD cord-298078-uqrwq5qk 93 19 ) ) -RRB- cord-298078-uqrwq5qk 93 20 . . . cord-298078-uqrwq5qk 94 1 Frameshifting frameshifte VBG cord-298078-uqrwq5qk 94 2 of of IN cord-298078-uqrwq5qk 94 3 the the DT cord-298078-uqrwq5qk 94 4 dual dual JJ cord-298078-uqrwq5qk 94 5 luciferase luciferase NN cord-298078-uqrwq5qk 94 6 reporter reporter NN cord-298078-uqrwq5qk 94 7 expressed express VBD cord-298078-uqrwq5qk 94 8 firefly firefly NN cord-298078-uqrwq5qk 94 9 luciferase luciferase NNP cord-298078-uqrwq5qk 94 10 as as RB cord-298078-uqrwq5qk 94 11 well well RB cord-298078-uqrwq5qk 94 12 as as IN cord-298078-uqrwq5qk 94 13 Renilla Renilla NNP cord-298078-uqrwq5qk 94 14 luciferase luciferase NN cord-298078-uqrwq5qk 94 15 ( ( -LRB- cord-298078-uqrwq5qk 94 16 Figure figure NN cord-298078-uqrwq5qk 94 17 4B 4b NN cord-298078-uqrwq5qk 94 18 ) ) -RRB- cord-298078-uqrwq5qk 94 19 , , , cord-298078-uqrwq5qk 94 20 so so IN cord-298078-uqrwq5qk 94 21 the the DT cord-298078-uqrwq5qk 94 22 frameshifting frameshifting NN cord-298078-uqrwq5qk 94 23 could could MD cord-298078-uqrwq5qk 94 24 be be VB cord-298078-uqrwq5qk 94 25 assessed assess VBN cord-298078-uqrwq5qk 94 26 by by IN cord-298078-uqrwq5qk 94 27 the the DT cord-298078-uqrwq5qk 94 28 ratio ratio NN cord-298078-uqrwq5qk 94 29 between between IN cord-298078-uqrwq5qk 94 30 these these DT cord-298078-uqrwq5qk 94 31 two two CD cord-298078-uqrwq5qk 94 32 proteins protein NNS cord-298078-uqrwq5qk 94 33 as as RB cord-298078-uqrwq5qk 94 34 well well RB cord-298078-uqrwq5qk 94 35 as as IN cord-298078-uqrwq5qk 94 36 by by IN cord-298078-uqrwq5qk 94 37 control control NN cord-298078-uqrwq5qk 94 38 reporters reporter NNS cord-298078-uqrwq5qk 94 39 . . . cord-298078-uqrwq5qk 95 1 Control control NN cord-298078-uqrwq5qk 95 2 reporters reporter NNS cord-298078-uqrwq5qk 95 3 with with IN cord-298078-uqrwq5qk 95 4 either either CC cord-298078-uqrwq5qk 95 5 in in IN cord-298078-uqrwq5qk 95 6 frame frame NN cord-298078-uqrwq5qk 95 7 or or CC cord-298078-uqrwq5qk 95 8 no no DT cord-298078-uqrwq5qk 95 9 expression expression NN cord-298078-uqrwq5qk 95 10 of of IN cord-298078-uqrwq5qk 95 11 firefly firefly NN cord-298078-uqrwq5qk 95 12 luciferase luciferase NN cord-298078-uqrwq5qk 95 13 used use VBN cord-298078-uqrwq5qk 95 14 as as IN cord-298078-uqrwq5qk 95 15 positive positive JJ cord-298078-uqrwq5qk 95 16 or or CC cord-298078-uqrwq5qk 95 17 negative negative JJ cord-298078-uqrwq5qk 95 18 controls control NNS cord-298078-uqrwq5qk 95 19 , , , cord-298078-uqrwq5qk 95 20 respectively respectively RB cord-298078-uqrwq5qk 95 21 . . . cord-298078-uqrwq5qk 96 1 To to TO cord-298078-uqrwq5qk 96 2 test test VB cord-298078-uqrwq5qk 96 3 how how WRB cord-298078-uqrwq5qk 96 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 96 5 regulates regulate VBZ cord-298078-uqrwq5qk 96 6 the the DT cord-298078-uqrwq5qk 96 7 frameshifting frameshifte VBG cord-298078-uqrwq5qk 96 8 efficiency efficiency NN cord-298078-uqrwq5qk 96 9 of of IN cord-298078-uqrwq5qk 96 10 IBV IBV NNP cord-298078-uqrwq5qk 96 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 96 12 RNA RNA NNP cord-298078-uqrwq5qk 96 13 , , , cord-298078-uqrwq5qk 96 14 we -PRON- PRP cord-298078-uqrwq5qk 96 15 first first RB cord-298078-uqrwq5qk 96 16 overexpressed overexpresse VBD cord-298078-uqrwq5qk 96 17 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 96 18 protein protein NN cord-298078-uqrwq5qk 96 19 in in IN cord-298078-uqrwq5qk 96 20 the the DT cord-298078-uqrwq5qk 96 21 presence presence NN cord-298078-uqrwq5qk 96 22 of of IN cord-298078-uqrwq5qk 96 23 the the DT cord-298078-uqrwq5qk 96 24 reporters reporter NNS cord-298078-uqrwq5qk 96 25 and and CC cord-298078-uqrwq5qk 96 26 measured measure VBD cord-298078-uqrwq5qk 96 27 the the DT cord-298078-uqrwq5qk 96 28 luciferase luciferase NN cord-298078-uqrwq5qk 96 29 activities activity NNS cord-298078-uqrwq5qk 96 30 . . . cord-298078-uqrwq5qk 97 1 Transfection transfection NN cord-298078-uqrwq5qk 97 2 of of IN cord-298078-uqrwq5qk 97 3 FLAGtagged flagtagged JJ cord-298078-uqrwq5qk 97 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 97 5 in in IN cord-298078-uqrwq5qk 97 6 HEK293 HEK293 NNP cord-298078-uqrwq5qk 97 7 T t NN cord-298078-uqrwq5qk 97 8 cells cell NNS cord-298078-uqrwq5qk 97 9 reduced reduce VBD cord-298078-uqrwq5qk 97 10 the the DT cord-298078-uqrwq5qk 97 11 relative relative JJ cord-298078-uqrwq5qk 97 12 frameshifting frameshifte VBG cord-298078-uqrwq5qk 97 13 efficiency efficiency NN cord-298078-uqrwq5qk 97 14 of of IN cord-298078-uqrwq5qk 97 15 IBV IBV NNP cord-298078-uqrwq5qk 97 16 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 97 17 RNA RNA NNP cord-298078-uqrwq5qk 97 18 up up IN cord-298078-uqrwq5qk 97 19 to to TO cord-298078-uqrwq5qk 97 20 40 40 CD cord-298078-uqrwq5qk 97 21 % % NN cord-298078-uqrwq5qk 97 22 ( ( -LRB- cord-298078-uqrwq5qk 97 23 Figure figure NN cord-298078-uqrwq5qk 97 24 5A 5a NN cord-298078-uqrwq5qk 97 25 ) ) -RRB- cord-298078-uqrwq5qk 97 26 . . . cord-298078-uqrwq5qk 98 1 Since since IN cord-298078-uqrwq5qk 98 2 HEK293 HEK293 NNP cord-298078-uqrwq5qk 98 3 T t NN cord-298078-uqrwq5qk 98 4 cells cell NNS cord-298078-uqrwq5qk 98 5 possess possess VBP cord-298078-uqrwq5qk 98 6 an an DT cord-298078-uqrwq5qk 98 7 endogenous endogenous JJ cord-298078-uqrwq5qk 98 8 protein protein NN cord-298078-uqrwq5qk 98 9 , , , cord-298078-uqrwq5qk 98 10 overexpression overexpression NN cord-298078-uqrwq5qk 98 11 of of IN cord-298078-uqrwq5qk 98 12 FLAG flag NN cord-298078-uqrwq5qk 98 13 - - HYPH cord-298078-uqrwq5qk 98 14 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 98 15 might may MD cord-298078-uqrwq5qk 98 16 generate generate VB cord-298078-uqrwq5qk 98 17 additional additional JJ cord-298078-uqrwq5qk 98 18 exogenous exogenous JJ cord-298078-uqrwq5qk 98 19 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 98 20 protein protein NN cord-298078-uqrwq5qk 98 21 . . . cord-298078-uqrwq5qk 99 1 To to TO cord-298078-uqrwq5qk 99 2 assess assess VB cord-298078-uqrwq5qk 99 3 more more RBR cord-298078-uqrwq5qk 99 4 definitively definitively RB cord-298078-uqrwq5qk 99 5 the the DT cord-298078-uqrwq5qk 99 6 inhibitory inhibitory JJ cord-298078-uqrwq5qk 99 7 effect effect NN cord-298078-uqrwq5qk 99 8 of of IN cord-298078-uqrwq5qk 99 9 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 99 10 on on IN cord-298078-uqrwq5qk 99 11 the the DT cord-298078-uqrwq5qk 99 12 frameshifting frameshifting NN cord-298078-uqrwq5qk 99 13 of of IN cord-298078-uqrwq5qk 99 14 IBV IBV NNP cord-298078-uqrwq5qk 99 15 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 99 16 RNA RNA NNP cord-298078-uqrwq5qk 99 17 , , , cord-298078-uqrwq5qk 99 18 we -PRON- PRP cord-298078-uqrwq5qk 99 19 used use VBD cord-298078-uqrwq5qk 99 20 a a DT cord-298078-uqrwq5qk 99 21 prostate prostate NN cord-298078-uqrwq5qk 99 22 cancer cancer NN cord-298078-uqrwq5qk 99 23 cell cell NN cord-298078-uqrwq5qk 99 24 line line NN cord-298078-uqrwq5qk 99 25 , , , cord-298078-uqrwq5qk 99 26 LNCaP lncap NN cord-298078-uqrwq5qk 99 27 , , , cord-298078-uqrwq5qk 99 28 which which WDT cord-298078-uqrwq5qk 99 29 does do VBZ cord-298078-uqrwq5qk 99 30 not not RB cord-298078-uqrwq5qk 99 31 express express VB cord-298078-uqrwq5qk 99 32 endogenous endogenous JJ cord-298078-uqrwq5qk 99 33 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 99 34 protein protein NN cord-298078-uqrwq5qk 99 35 in in IN cord-298078-uqrwq5qk 99 36 the the DT cord-298078-uqrwq5qk 99 37 cells cell NNS cord-298078-uqrwq5qk 99 38 . . . cord-298078-uqrwq5qk 100 1 More more RBR cord-298078-uqrwq5qk 100 2 significant significant JJ cord-298078-uqrwq5qk 100 3 reduction reduction NN cord-298078-uqrwq5qk 100 4 of of IN cord-298078-uqrwq5qk 100 5 the the DT cord-298078-uqrwq5qk 100 6 relative relative JJ cord-298078-uqrwq5qk 100 7 frameshifting frameshifte VBG cord-298078-uqrwq5qk 100 8 efficiency efficiency NN cord-298078-uqrwq5qk 100 9 was be VBD cord-298078-uqrwq5qk 100 10 shown show VBN cord-298078-uqrwq5qk 100 11 in in IN cord-298078-uqrwq5qk 100 12 LNCaP lncap NN cord-298078-uqrwq5qk 100 13 cells cell NNS cord-298078-uqrwq5qk 100 14 , , , cord-298078-uqrwq5qk 100 15 which which WDT cord-298078-uqrwq5qk 100 16 likely likely RB cord-298078-uqrwq5qk 100 17 resulted result VBD cord-298078-uqrwq5qk 100 18 from from IN cord-298078-uqrwq5qk 100 19 the the DT cord-298078-uqrwq5qk 100 20 overexpression overexpression NN cord-298078-uqrwq5qk 100 21 of of IN cord-298078-uqrwq5qk 100 22 ANXA2 ANXA2 NNS cord-298078-uqrwq5qk 100 23 without without IN cord-298078-uqrwq5qk 100 24 any any DT cord-298078-uqrwq5qk 100 25 basal basal NN cord-298078-uqrwq5qk 100 26 level level NN cord-298078-uqrwq5qk 100 27 expression expression NN cord-298078-uqrwq5qk 100 28 of of IN cord-298078-uqrwq5qk 100 29 the the DT cord-298078-uqrwq5qk 100 30 protein protein NN cord-298078-uqrwq5qk 100 31 ( ( -LRB- cord-298078-uqrwq5qk 100 32 Figure figure NN cord-298078-uqrwq5qk 100 33 5B 5b NN cord-298078-uqrwq5qk 100 34 ) ) -RRB- cord-298078-uqrwq5qk 100 35 . . . cord-298078-uqrwq5qk 101 1 Conversely conversely RB cord-298078-uqrwq5qk 101 2 , , , cord-298078-uqrwq5qk 101 3 we -PRON- PRP cord-298078-uqrwq5qk 101 4 showed show VBD cord-298078-uqrwq5qk 101 5 that that IN cord-298078-uqrwq5qk 101 6 the the DT cord-298078-uqrwq5qk 101 7 downregulation downregulation NN cord-298078-uqrwq5qk 101 8 of of IN cord-298078-uqrwq5qk 101 9 the the DT cord-298078-uqrwq5qk 101 10 ANXA2 anxa2 NN cord-298078-uqrwq5qk 101 11 by by IN cord-298078-uqrwq5qk 101 12 three three CD cord-298078-uqrwq5qk 101 13 independent independent JJ cord-298078-uqrwq5qk 101 14 knockdown knockdown NN cord-298078-uqrwq5qk 101 15 greatly greatly RB cord-298078-uqrwq5qk 101 16 increased increase VBD cord-298078-uqrwq5qk 101 17 the the DT cord-298078-uqrwq5qk 101 18 relative relative JJ cord-298078-uqrwq5qk 101 19 frameshifting frameshifte VBG cord-298078-uqrwq5qk 101 20 efficiency efficiency NN cord-298078-uqrwq5qk 101 21 of of IN cord-298078-uqrwq5qk 101 22 IBV IBV NNP cord-298078-uqrwq5qk 101 23 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 101 24 RNA RNA NNP cord-298078-uqrwq5qk 101 25 in in IN cord-298078-uqrwq5qk 101 26 HEK293 HEK293 NNP cord-298078-uqrwq5qk 101 27 T t NN cord-298078-uqrwq5qk 101 28 cells cell NNS cord-298078-uqrwq5qk 101 29 ( ( -LRB- cord-298078-uqrwq5qk 101 30 Figure figure NN cord-298078-uqrwq5qk 101 31 5D 5d NN cord-298078-uqrwq5qk 101 32 and and CC cord-298078-uqrwq5qk 101 33 5E 5e NN cord-298078-uqrwq5qk 101 34 ) ) -RRB- cord-298078-uqrwq5qk 101 35 . . . cord-298078-uqrwq5qk 102 1 Targeting target VBG cord-298078-uqrwq5qk 102 2 sequences sequence NNS cord-298078-uqrwq5qk 102 3 of of IN cord-298078-uqrwq5qk 102 4 shRNA shrna NN cord-298078-uqrwq5qk 102 5 and and CC cord-298078-uqrwq5qk 102 6 siRNA siRNA NNP cord-298078-uqrwq5qk 102 7 in in IN cord-298078-uqrwq5qk 103 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 103 2 mRNA mRNA NNP cord-298078-uqrwq5qk 103 3 were be VBD cord-298078-uqrwq5qk 103 4 diagrammed diagram VBN cord-298078-uqrwq5qk 103 5 in in IN cord-298078-uqrwq5qk 103 6 Figure figure NN cord-298078-uqrwq5qk 103 7 5C 5c CD cord-298078-uqrwq5qk 103 8 . . . cord-298078-uqrwq5qk 104 1 By by IN cord-298078-uqrwq5qk 104 2 independent independent JJ cord-298078-uqrwq5qk 104 3 experiments experiment NNS cord-298078-uqrwq5qk 104 4 either either CC cord-298078-uqrwq5qk 104 5 with with IN cord-298078-uqrwq5qk 104 6 shRNA shrna JJ cord-298078-uqrwq5qk 104 7 expression expression NN cord-298078-uqrwq5qk 104 8 vector vector NN cord-298078-uqrwq5qk 104 9 or or CC cord-298078-uqrwq5qk 104 10 with with IN cord-298078-uqrwq5qk 104 11 siRNA siRNA NNP cord-298078-uqrwq5qk 104 12 duplex duplex NNP cord-298078-uqrwq5qk 104 13 , , , cord-298078-uqrwq5qk 104 14 we -PRON- PRP cord-298078-uqrwq5qk 104 15 clearly clearly RB cord-298078-uqrwq5qk 104 16 demonstrated demonstrate VBD cord-298078-uqrwq5qk 104 17 that that IN cord-298078-uqrwq5qk 104 18 frameshifting frameshifte VBG cord-298078-uqrwq5qk 104 19 efficiency efficiency NN cord-298078-uqrwq5qk 104 20 was be VBD cord-298078-uqrwq5qk 104 21 dramatically dramatically RB cord-298078-uqrwq5qk 104 22 increased increase VBN cord-298078-uqrwq5qk 104 23 by by IN cord-298078-uqrwq5qk 104 24 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 104 25 knockdown knockdown NN cord-298078-uqrwq5qk 104 26 . . . cord-298078-uqrwq5qk 105 1 These these DT cord-298078-uqrwq5qk 105 2 data datum NNS cord-298078-uqrwq5qk 105 3 clearly clearly RB cord-298078-uqrwq5qk 105 4 indicated indicate VBD cord-298078-uqrwq5qk 105 5 that that IN cord-298078-uqrwq5qk 105 6 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 105 7 controls control VBZ cord-298078-uqrwq5qk 105 8 the the DT cord-298078-uqrwq5qk 105 9 frameshifting frameshifte VBG cord-298078-uqrwq5qk 105 10 efficiency efficiency NN cord-298078-uqrwq5qk 105 11 in in IN cord-298078-uqrwq5qk 105 12 the the DT cord-298078-uqrwq5qk 105 13 cells cell NNS cord-298078-uqrwq5qk 105 14 , , , cord-298078-uqrwq5qk 105 15 which which WDT cord-298078-uqrwq5qk 105 16 could could MD cord-298078-uqrwq5qk 105 17 act act VB cord-298078-uqrwq5qk 105 18 as as IN cord-298078-uqrwq5qk 105 19 the the DT cord-298078-uqrwq5qk 105 20 cellular cellular JJ cord-298078-uqrwq5qk 105 21 inhibitor inhibitor NN cord-298078-uqrwq5qk 105 22 against against IN cord-298078-uqrwq5qk 105 23 the the DT cord-298078-uqrwq5qk 105 24 frameshifting frameshifting NN cord-298078-uqrwq5qk 105 25 of of IN cord-298078-uqrwq5qk 105 26 viral viral JJ cord-298078-uqrwq5qk 105 27 RNA RNA NNP cord-298078-uqrwq5qk 105 28 translation translation NN cord-298078-uqrwq5qk 105 29 . . . cord-298078-uqrwq5qk 106 1 Translation translation NN cord-298078-uqrwq5qk 106 2 is be VBZ cord-298078-uqrwq5qk 106 3 the the DT cord-298078-uqrwq5qk 106 4 complex complex JJ cord-298078-uqrwq5qk 106 5 RNA RNA NNP cord-298078-uqrwq5qk 106 6 metabolism metabolism NN cord-298078-uqrwq5qk 106 7 regulated regulate VBN cord-298078-uqrwq5qk 106 8 by by IN cord-298078-uqrwq5qk 106 9 diverse diverse JJ cord-298078-uqrwq5qk 106 10 factors factor NNS cord-298078-uqrwq5qk 106 11 , , , cord-298078-uqrwq5qk 106 12 such such JJ cord-298078-uqrwq5qk 106 13 as as IN cord-298078-uqrwq5qk 106 14 ribosome ribosome NN cord-298078-uqrwq5qk 106 15 , , , cord-298078-uqrwq5qk 106 16 tRNA trna NN cord-298078-uqrwq5qk 106 17 and and CC cord-298078-uqrwq5qk 106 18 many many JJ cord-298078-uqrwq5qk 106 19 ribosome ribosome NN cord-298078-uqrwq5qk 106 20 associated associate VBD cord-298078-uqrwq5qk 106 21 proteins protein NNS cord-298078-uqrwq5qk 106 22 . . . cord-298078-uqrwq5qk 107 1 Here here RB cord-298078-uqrwq5qk 107 2 , , , cord-298078-uqrwq5qk 107 3 we -PRON- PRP cord-298078-uqrwq5qk 107 4 identify identify VBP cord-298078-uqrwq5qk 107 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 107 6 as as IN cord-298078-uqrwq5qk 107 7 the the DT cord-298078-uqrwq5qk 107 8 regulator regulator NN cord-298078-uqrwq5qk 107 9 of of IN cord-298078-uqrwq5qk 107 10 ribosomal ribosomal JJ cord-298078-uqrwq5qk 107 11 frameshifting frameshifting NN cord-298078-uqrwq5qk 107 12 . . . cord-298078-uqrwq5qk 108 1 Since since IN cord-298078-uqrwq5qk 108 2 it -PRON- PRP cord-298078-uqrwq5qk 108 3 was be VBD cord-298078-uqrwq5qk 108 4 previously previously RB cord-298078-uqrwq5qk 108 5 reported report VBN cord-298078-uqrwq5qk 108 6 that that IN cord-298078-uqrwq5qk 108 7 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 108 8 binds bind VBZ cord-298078-uqrwq5qk 108 9 RNA RNA NNP cord-298078-uqrwq5qk 108 10 and and CC cord-298078-uqrwq5qk 108 11 regulates regulate VBZ cord-298078-uqrwq5qk 108 12 RNA RNA NNP cord-298078-uqrwq5qk 108 13 localization localization NN cord-298078-uqrwq5qk 108 14 [ [ -LRB- cord-298078-uqrwq5qk 108 15 13 13 CD cord-298078-uqrwq5qk 108 16 , , , cord-298078-uqrwq5qk 108 17 14 14 CD cord-298078-uqrwq5qk 108 18 , , , cord-298078-uqrwq5qk 108 19 15 15 CD cord-298078-uqrwq5qk 108 20 , , , cord-298078-uqrwq5qk 108 21 16 16 CD cord-298078-uqrwq5qk 108 22 , , , cord-298078-uqrwq5qk 108 23 17 17 CD cord-298078-uqrwq5qk 108 24 ] ] -RRB- cord-298078-uqrwq5qk 108 25 , , , cord-298078-uqrwq5qk 108 26 our -PRON- PRP$ cord-298078-uqrwq5qk 108 27 proteomic proteomic JJ cord-298078-uqrwq5qk 108 28 identification identification NN cord-298078-uqrwq5qk 108 29 of of IN cord-298078-uqrwq5qk 108 30 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 108 31 as as IN cord-298078-uqrwq5qk 108 32 an an DT cord-298078-uqrwq5qk 108 33 IBV IBV NNP cord-298078-uqrwq5qk 108 34 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 108 35 RNA RNA NNP cord-298078-uqrwq5qk 108 36 binding bind VBG cord-298078-uqrwq5qk 108 37 protein protein NN cord-298078-uqrwq5qk 108 38 leads lead VBZ cord-298078-uqrwq5qk 108 39 us -PRON- PRP cord-298078-uqrwq5qk 108 40 to to TO cord-298078-uqrwq5qk 108 41 propose propose VB cord-298078-uqrwq5qk 108 42 that that IN cord-298078-uqrwq5qk 108 43 it -PRON- PRP cord-298078-uqrwq5qk 108 44 might may MD cord-298078-uqrwq5qk 108 45 regulate regulate VB cord-298078-uqrwq5qk 108 46 the the DT cord-298078-uqrwq5qk 108 47 frameshifting frameshifte VBG cord-298078-uqrwq5qk 108 48 efficiency efficiency NN cord-298078-uqrwq5qk 108 49 of of IN cord-298078-uqrwq5qk 108 50 viral viral JJ cord-298078-uqrwq5qk 108 51 RNA RNA NNP cord-298078-uqrwq5qk 108 52 translation translation NN cord-298078-uqrwq5qk 108 53 . . . cord-298078-uqrwq5qk 109 1 As as IN cord-298078-uqrwq5qk 109 2 shown show VBN cord-298078-uqrwq5qk 109 3 in in IN cord-298078-uqrwq5qk 109 4 Figure Figure NNP cord-298078-uqrwq5qk 109 5 5 5 CD cord-298078-uqrwq5qk 109 6 , , , cord-298078-uqrwq5qk 109 7 knockdown knockdown NN cord-298078-uqrwq5qk 109 8 of of IN cord-298078-uqrwq5qk 109 9 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 109 10 increased increase VBD cord-298078-uqrwq5qk 109 11 the the DT cord-298078-uqrwq5qk 109 12 frameshifting frameshifte VBG cord-298078-uqrwq5qk 109 13 efficiency efficiency NN cord-298078-uqrwq5qk 109 14 up up IN cord-298078-uqrwq5qk 109 15 to to TO cord-298078-uqrwq5qk 109 16 seven seven CD cord-298078-uqrwq5qk 109 17 times time NNS cord-298078-uqrwq5qk 109 18 , , , cord-298078-uqrwq5qk 109 19 whereas whereas IN cord-298078-uqrwq5qk 109 20 overexpression overexpression NN cord-298078-uqrwq5qk 109 21 of of IN cord-298078-uqrwq5qk 109 22 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 109 23 significantly significantly RB cord-298078-uqrwq5qk 109 24 down down RB cord-298078-uqrwq5qk 109 25 - - HYPH cord-298078-uqrwq5qk 109 26 regulated regulate VBD cord-298078-uqrwq5qk 109 27 the the DT cord-298078-uqrwq5qk 109 28 frameshifting frameshifte VBG cord-298078-uqrwq5qk 109 29 efficiency efficiency NN cord-298078-uqrwq5qk 109 30 of of IN cord-298078-uqrwq5qk 109 31 IBV IBV NNP cord-298078-uqrwq5qk 109 32 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 109 33 RNA RNA NNP cord-298078-uqrwq5qk 109 34 . . . cord-298078-uqrwq5qk 110 1 These these DT cord-298078-uqrwq5qk 110 2 results result NNS cord-298078-uqrwq5qk 110 3 clearly clearly RB cord-298078-uqrwq5qk 110 4 show show VBP cord-298078-uqrwq5qk 110 5 that that IN cord-298078-uqrwq5qk 110 6 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 110 7 reduces reduce VBZ cord-298078-uqrwq5qk 110 8 IBV IBV NNP cord-298078-uqrwq5qk 110 9 viral viral JJ cord-298078-uqrwq5qk 110 10 frameshifting frameshifte VBG cord-298078-uqrwq5qk 110 11 in in IN cord-298078-uqrwq5qk 110 12 some some DT cord-298078-uqrwq5qk 110 13 way way NN cord-298078-uqrwq5qk 110 14 , , , cord-298078-uqrwq5qk 110 15 probably probably RB cord-298078-uqrwq5qk 110 16 by by IN cord-298078-uqrwq5qk 110 17 binding bind VBG cord-298078-uqrwq5qk 110 18 to to IN cord-298078-uqrwq5qk 110 19 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 110 20 RNA RNA NNP cord-298078-uqrwq5qk 110 21 . . . cord-298078-uqrwq5qk 111 1 Since since IN cord-298078-uqrwq5qk 111 2 the the DT cord-298078-uqrwq5qk 111 3 frameshifting frameshifting NN cord-298078-uqrwq5qk 111 4 of of IN cord-298078-uqrwq5qk 111 5 IBV IBV NNP cord-298078-uqrwq5qk 111 6 generates generate VBZ cord-298078-uqrwq5qk 111 7 RNA RNA NNP cord-298078-uqrwq5qk 111 8 dependent dependent JJ cord-298078-uqrwq5qk 111 9 RNA RNA NNP cord-298078-uqrwq5qk 111 10 polymerase polymerase NN cord-298078-uqrwq5qk 111 11 , , , cord-298078-uqrwq5qk 111 12 which which WDT cord-298078-uqrwq5qk 111 13 is be VBZ cord-298078-uqrwq5qk 111 14 critical critical JJ cord-298078-uqrwq5qk 111 15 for for IN cord-298078-uqrwq5qk 111 16 successful successful JJ cord-298078-uqrwq5qk 111 17 viral viral JJ cord-298078-uqrwq5qk 111 18 replication replication NN cord-298078-uqrwq5qk 111 19 , , , cord-298078-uqrwq5qk 111 20 the the DT cord-298078-uqrwq5qk 111 21 protein protein NN cord-298078-uqrwq5qk 111 22 that that WDT cord-298078-uqrwq5qk 111 23 inhibits inhibit VBZ cord-298078-uqrwq5qk 111 24 frameshifting frameshifting NN cord-298078-uqrwq5qk 111 25 must must MD cord-298078-uqrwq5qk 111 26 be be VB cord-298078-uqrwq5qk 111 27 a a DT cord-298078-uqrwq5qk 111 28 major major JJ cord-298078-uqrwq5qk 111 29 antiviral antiviral JJ cord-298078-uqrwq5qk 111 30 regulator regulator NN cord-298078-uqrwq5qk 111 31 in in IN cord-298078-uqrwq5qk 111 32 eukaryotic eukaryotic JJ cord-298078-uqrwq5qk 111 33 cells cell NNS cord-298078-uqrwq5qk 111 34 . . . cord-298078-uqrwq5qk 112 1 However however RB cord-298078-uqrwq5qk 112 2 , , , cord-298078-uqrwq5qk 112 3 many many JJ cord-298078-uqrwq5qk 112 4 questions question NNS cord-298078-uqrwq5qk 112 5 remain remain VBP cord-298078-uqrwq5qk 112 6 to to TO cord-298078-uqrwq5qk 112 7 be be VB cord-298078-uqrwq5qk 112 8 answered answer VBN cord-298078-uqrwq5qk 112 9 . . . cord-298078-uqrwq5qk 113 1 These these DT cord-298078-uqrwq5qk 113 2 include include VBP cord-298078-uqrwq5qk 113 3 the the DT cord-298078-uqrwq5qk 113 4 exact exact JJ cord-298078-uqrwq5qk 113 5 mechanism mechanism NN cord-298078-uqrwq5qk 113 6 of of IN cord-298078-uqrwq5qk 113 7 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 113 8 action action NN cord-298078-uqrwq5qk 113 9 on on IN cord-298078-uqrwq5qk 113 10 IBV IBV NNP cord-298078-uqrwq5qk 113 11 frameshifting frameshifte VBG cord-298078-uqrwq5qk 113 12 , , , cord-298078-uqrwq5qk 113 13 possible possible JJ cord-298078-uqrwq5qk 113 14 involvement involvement NN cord-298078-uqrwq5qk 113 15 of of IN cord-298078-uqrwq5qk 113 16 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 113 17 on on IN cord-298078-uqrwq5qk 113 18 frameshifting frameshifte VBG cord-298078-uqrwq5qk 113 19 of of IN cord-298078-uqrwq5qk 113 20 other other JJ cord-298078-uqrwq5qk 113 21 viral viral JJ cord-298078-uqrwq5qk 113 22 RNA RNA NNP cord-298078-uqrwq5qk 113 23 and and CC cord-298078-uqrwq5qk 113 24 unexplored unexplored JJ cord-298078-uqrwq5qk 113 25 functions function NNS cord-298078-uqrwq5qk 113 26 of of IN cord-298078-uqrwq5qk 113 27 RNA RNA NNP cord-298078-uqrwq5qk 113 28 metabolism metabolism NN cord-298078-uqrwq5qk 113 29 on on IN cord-298078-uqrwq5qk 113 30 many many JJ cord-298078-uqrwq5qk 113 31 other other JJ cord-298078-uqrwq5qk 113 32 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 113 33 containing contain VBG cord-298078-uqrwq5qk 113 34 cellular cellular JJ cord-298078-uqrwq5qk 113 35 RNA RNA NNP cord-298078-uqrwq5qk 113 36 . . . cord-298078-uqrwq5qk 114 1 RNA RNA NNP cord-298078-uqrwq5qk 114 2 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 114 3 are be VBP cord-298078-uqrwq5qk 114 4 structural structural JJ cord-298078-uqrwq5qk 114 5 elements element NNS cord-298078-uqrwq5qk 114 6 formed form VBN cord-298078-uqrwq5qk 114 7 upon upon IN cord-298078-uqrwq5qk 114 8 basepairing basepairing NN cord-298078-uqrwq5qk 114 9 of of IN cord-298078-uqrwq5qk 114 10 a a DT cord-298078-uqrwq5qk 114 11 single single RB cord-298078-uqrwq5qk 114 12 - - HYPH cord-298078-uqrwq5qk 114 13 stranded strand VBN cord-298078-uqrwq5qk 114 14 region region NN cord-298078-uqrwq5qk 114 15 to to IN cord-298078-uqrwq5qk 114 16 a a DT cord-298078-uqrwq5qk 114 17 stretch stretch NN cord-298078-uqrwq5qk 114 18 of of IN cord-298078-uqrwq5qk 114 19 complementary complementary JJ cord-298078-uqrwq5qk 114 20 nucleotides nucleotide NNS cord-298078-uqrwq5qk 114 21 elsewhere elsewhere RB cord-298078-uqrwq5qk 114 22 in in IN cord-298078-uqrwq5qk 114 23 the the DT cord-298078-uqrwq5qk 114 24 RNA RNA NNP cord-298078-uqrwq5qk 114 25 chain chain NN cord-298078-uqrwq5qk 114 26 . . . cord-298078-uqrwq5qk 115 1 Since since IN cord-298078-uqrwq5qk 115 2 the the DT cord-298078-uqrwq5qk 115 3 first first JJ cord-298078-uqrwq5qk 115 4 discovery discovery NN cord-298078-uqrwq5qk 115 5 of of IN cord-298078-uqrwq5qk 115 6 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 115 7 structure structure NN cord-298078-uqrwq5qk 115 8 in in IN cord-298078-uqrwq5qk 115 9 39-UTR 39-utr CD cord-298078-uqrwq5qk 115 10 of of IN cord-298078-uqrwq5qk 115 11 Turnip Turnip NNP cord-298078-uqrwq5qk 115 12 Yellow Yellow NNP cord-298078-uqrwq5qk 115 13 Mosaic Mosaic NNP cord-298078-uqrwq5qk 115 14 Virus Virus NNP cord-298078-uqrwq5qk 115 15 ( ( -LRB- cord-298078-uqrwq5qk 115 16 TYMV TYMV NNP cord-298078-uqrwq5qk 115 17 ) ) -RRB- cord-298078-uqrwq5qk 115 18 , , , cord-298078-uqrwq5qk 115 19 numerous numerous JJ cord-298078-uqrwq5qk 115 20 viruses virus NNS cord-298078-uqrwq5qk 115 21 have have VBP cord-298078-uqrwq5qk 115 22 been be VBN cord-298078-uqrwq5qk 115 23 reported report VBN cord-298078-uqrwq5qk 115 24 to to TO cord-298078-uqrwq5qk 115 25 contain contain VB cord-298078-uqrwq5qk 115 26 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 115 27 RNA RNA NNP cord-298078-uqrwq5qk 115 28 [ [ -LRB- cord-298078-uqrwq5qk 115 29 7 7 CD cord-298078-uqrwq5qk 115 30 , , , cord-298078-uqrwq5qk 115 31 8 8 CD cord-298078-uqrwq5qk 115 32 , , , cord-298078-uqrwq5qk 115 33 9 9 CD cord-298078-uqrwq5qk 115 34 ] ] -RRB- cord-298078-uqrwq5qk 115 35 . . . cord-298078-uqrwq5qk 116 1 Many many JJ cord-298078-uqrwq5qk 116 2 other other JJ cord-298078-uqrwq5qk 116 3 cellular cellular JJ cord-298078-uqrwq5qk 116 4 RNAs rna NNS cord-298078-uqrwq5qk 116 5 , , , cord-298078-uqrwq5qk 116 6 such such JJ cord-298078-uqrwq5qk 116 7 as as IN cord-298078-uqrwq5qk 116 8 rRNA rRNA NNP cord-298078-uqrwq5qk 116 9 , , , cord-298078-uqrwq5qk 116 10 mRNA mRNA NNP cord-298078-uqrwq5qk 116 11 , , , cord-298078-uqrwq5qk 116 12 tmRNA tmRNA NNP cord-298078-uqrwq5qk 116 13 , , , cord-298078-uqrwq5qk 116 14 catalytic catalytic NNP cord-298078-uqrwq5qk 116 15 RNA RNA NNP cord-298078-uqrwq5qk 116 16 , , , cord-298078-uqrwq5qk 116 17 telomerase telomerase NN cord-298078-uqrwq5qk 116 18 RNA RNA NNP cord-298078-uqrwq5qk 116 19 , , , cord-298078-uqrwq5qk 116 20 RNA RNA NNP cord-298078-uqrwq5qk 116 21 components component NNS cord-298078-uqrwq5qk 116 22 of of IN cord-298078-uqrwq5qk 116 23 ribonucleoprotein ribonucleoprotein NN cord-298078-uqrwq5qk 116 24 complex complex NN cord-298078-uqrwq5qk 116 25 and and CC cord-298078-uqrwq5qk 116 26 many many JJ cord-298078-uqrwq5qk 116 27 artificially artificially RB cord-298078-uqrwq5qk 116 28 selected select VBN cord-298078-uqrwq5qk 116 29 RNA RNA NNP cord-298078-uqrwq5qk 116 30 aptamers aptamer NNS cord-298078-uqrwq5qk 116 31 also also RB cord-298078-uqrwq5qk 116 32 form form VBP cord-298078-uqrwq5qk 116 33 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 116 34 structures structure NNS cord-298078-uqrwq5qk 116 35 [ [ -LRB- cord-298078-uqrwq5qk 116 36 23 23 CD cord-298078-uqrwq5qk 116 37 , , , cord-298078-uqrwq5qk 116 38 24 24 CD cord-298078-uqrwq5qk 116 39 , , , cord-298078-uqrwq5qk 116 40 25 25 CD cord-298078-uqrwq5qk 116 41 , , , cord-298078-uqrwq5qk 116 42 26 26 CD cord-298078-uqrwq5qk 116 43 , , , cord-298078-uqrwq5qk 116 44 27 27 CD cord-298078-uqrwq5qk 116 45 ] ] -RRB- cord-298078-uqrwq5qk 116 46 . . . cord-298078-uqrwq5qk 117 1 A a DT cord-298078-uqrwq5qk 117 2 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 117 3 is be VBZ cord-298078-uqrwq5qk 117 4 not not RB cord-298078-uqrwq5qk 117 5 only only RB cord-298078-uqrwq5qk 117 6 a a DT cord-298078-uqrwq5qk 117 7 structural structural JJ cord-298078-uqrwq5qk 117 8 element element NN cord-298078-uqrwq5qk 117 9 of of IN cord-298078-uqrwq5qk 117 10 RNA RNA NNP cord-298078-uqrwq5qk 117 11 , , , cord-298078-uqrwq5qk 117 12 but but CC cord-298078-uqrwq5qk 117 13 also also RB cord-298078-uqrwq5qk 117 14 a a DT cord-298078-uqrwq5qk 117 15 functional functional JJ cord-298078-uqrwq5qk 117 16 regulator regulator NN cord-298078-uqrwq5qk 117 17 of of IN cord-298078-uqrwq5qk 117 18 RNA RNA NNP cord-298078-uqrwq5qk 117 19 . . . cord-298078-uqrwq5qk 118 1 As as IN cord-298078-uqrwq5qk 118 2 mentioned mention VBN cord-298078-uqrwq5qk 118 3 previously previously RB cord-298078-uqrwq5qk 118 4 , , , cord-298078-uqrwq5qk 118 5 RNA RNA NNP cord-298078-uqrwq5qk 118 6 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 118 7 located locate VBN cord-298078-uqrwq5qk 118 8 in in IN cord-298078-uqrwq5qk 118 9 the the DT cord-298078-uqrwq5qk 118 10 coding code VBG cord-298078-uqrwq5qk 118 11 region region NN cord-298078-uqrwq5qk 118 12 of of IN cord-298078-uqrwq5qk 118 13 virus virus NN cord-298078-uqrwq5qk 118 14 is be VBZ cord-298078-uqrwq5qk 118 15 involved involve VBN cord-298078-uqrwq5qk 118 16 in in IN cord-298078-uqrwq5qk 118 17 the the DT cord-298078-uqrwq5qk 118 18 -1 -1 NN cord-298078-uqrwq5qk 118 19 ribosomal ribosomal JJ cord-298078-uqrwq5qk 118 20 frameshifting frameshifting NN cord-298078-uqrwq5qk 118 21 of of IN cord-298078-uqrwq5qk 118 22 viral viral JJ cord-298078-uqrwq5qk 118 23 RNA RNA NNP cord-298078-uqrwq5qk 118 24 translation translation NN cord-298078-uqrwq5qk 118 25 , , , cord-298078-uqrwq5qk 118 26 and and CC cord-298078-uqrwq5qk 118 27 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 118 28 in in IN cord-298078-uqrwq5qk 118 29 59-UTR 59-utr CD cord-298078-uqrwq5qk 118 30 in in IN cord-298078-uqrwq5qk 118 31 some some DT cord-298078-uqrwq5qk 118 32 cases case NNS cord-298078-uqrwq5qk 118 33 regulates regulate VBZ cord-298078-uqrwq5qk 118 34 the the DT cord-298078-uqrwq5qk 118 35 initiation initiation NN cord-298078-uqrwq5qk 118 36 of of IN cord-298078-uqrwq5qk 118 37 viral viral JJ cord-298078-uqrwq5qk 118 38 RNA RNA NNP cord-298078-uqrwq5qk 118 39 replication replication NN cord-298078-uqrwq5qk 118 40 [ [ -LRB- cord-298078-uqrwq5qk 118 41 28 28 CD cord-298078-uqrwq5qk 118 42 ] ] -RRB- cord-298078-uqrwq5qk 118 43 . . . cord-298078-uqrwq5qk 119 1 Since since IN cord-298078-uqrwq5qk 119 2 mutational mutational JJ cord-298078-uqrwq5qk 119 3 analysis analysis NN cord-298078-uqrwq5qk 119 4 revealed reveal VBD cord-298078-uqrwq5qk 119 5 drastic drastic JJ cord-298078-uqrwq5qk 119 6 changes change NNS cord-298078-uqrwq5qk 119 7 of of IN cord-298078-uqrwq5qk 119 8 ribosomal ribosomal JJ cord-298078-uqrwq5qk 119 9 frameshifting frameshifte VBG cord-298078-uqrwq5qk 119 10 efficiency efficiency NN cord-298078-uqrwq5qk 119 11 upon upon IN cord-298078-uqrwq5qk 119 12 disruption disruption NN cord-298078-uqrwq5qk 119 13 of of IN cord-298078-uqrwq5qk 119 14 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 119 15 structure structure NN cord-298078-uqrwq5qk 119 16 , , , cord-298078-uqrwq5qk 119 17 this this DT cord-298078-uqrwq5qk 119 18 structure structure NN cord-298078-uqrwq5qk 119 19 is be VBZ cord-298078-uqrwq5qk 119 20 likely likely JJ cord-298078-uqrwq5qk 119 21 to to TO cord-298078-uqrwq5qk 119 22 be be VB cord-298078-uqrwq5qk 119 23 kept keep VBN cord-298078-uqrwq5qk 119 24 intact intact JJ cord-298078-uqrwq5qk 119 25 in in IN cord-298078-uqrwq5qk 119 26 addition addition NN cord-298078-uqrwq5qk 119 27 to to IN cord-298078-uqrwq5qk 119 28 the the DT cord-298078-uqrwq5qk 119 29 slippery slippery JJ cord-298078-uqrwq5qk 119 30 site site NN cord-298078-uqrwq5qk 119 31 and and CC cord-298078-uqrwq5qk 119 32 spacer spacer NN cord-298078-uqrwq5qk 119 33 region region NN cord-298078-uqrwq5qk 119 34 for for IN cord-298078-uqrwq5qk 119 35 efficient efficient JJ cord-298078-uqrwq5qk 119 36 frameshifting frameshifte VBG cord-298078-uqrwq5qk 119 37 [ [ -LRB- cord-298078-uqrwq5qk 119 38 29 29 CD cord-298078-uqrwq5qk 119 39 ] ] -RRB- cord-298078-uqrwq5qk 119 40 . . . cord-298078-uqrwq5qk 120 1 Recent recent JJ cord-298078-uqrwq5qk 120 2 advances advance NNS cord-298078-uqrwq5qk 120 3 in in IN cord-298078-uqrwq5qk 120 4 RNA RNA NNP cord-298078-uqrwq5qk 120 5 bioinformatics bioinformatic NNS cord-298078-uqrwq5qk 120 6 broaden broaden VBP cord-298078-uqrwq5qk 120 7 the the DT cord-298078-uqrwq5qk 120 8 possibility possibility NN cord-298078-uqrwq5qk 120 9 of of IN cord-298078-uqrwq5qk 120 10 finding find VBG cord-298078-uqrwq5qk 120 11 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 120 12 structure structure NN cord-298078-uqrwq5qk 120 13 from from IN cord-298078-uqrwq5qk 120 14 RNA RNA NNP cord-298078-uqrwq5qk 120 15 sequences sequence NNS cord-298078-uqrwq5qk 120 16 and and CC cord-298078-uqrwq5qk 120 17 predicting predict VBG cord-298078-uqrwq5qk 120 18 threedimensional threedimensional JJ cord-298078-uqrwq5qk 120 19 structures structure NNS cord-298078-uqrwq5qk 120 20 of of IN cord-298078-uqrwq5qk 120 21 the the DT cord-298078-uqrwq5qk 120 22 RNA RNA NNP cord-298078-uqrwq5qk 120 23 molecules molecule NNS cord-298078-uqrwq5qk 120 24 [ [ -LRB- cord-298078-uqrwq5qk 120 25 30 30 CD cord-298078-uqrwq5qk 120 26 , , , cord-298078-uqrwq5qk 120 27 31 31 CD cord-298078-uqrwq5qk 120 28 , , , cord-298078-uqrwq5qk 120 29 32 32 CD cord-298078-uqrwq5qk 120 30 ] ] -RRB- cord-298078-uqrwq5qk 120 31 . . . cord-298078-uqrwq5qk 121 1 The the DT cord-298078-uqrwq5qk 121 2 detailed detailed JJ cord-298078-uqrwq5qk 121 3 mechanism mechanism NN cord-298078-uqrwq5qk 121 4 of of IN cord-298078-uqrwq5qk 121 5 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 121 6 function function VB cord-298078-uqrwq5qk 121 7 on on IN cord-298078-uqrwq5qk 121 8 frameshifting frameshifte VBG cord-298078-uqrwq5qk 121 9 is be VBZ cord-298078-uqrwq5qk 121 10 being be VBG cord-298078-uqrwq5qk 121 11 intensively intensively RB cord-298078-uqrwq5qk 121 12 studied study VBN cord-298078-uqrwq5qk 121 13 . . . cord-298078-uqrwq5qk 122 1 Recent recent JJ cord-298078-uqrwq5qk 122 2 data datum NNS cord-298078-uqrwq5qk 122 3 suggests suggest VBZ cord-298078-uqrwq5qk 122 4 that that IN cord-298078-uqrwq5qk 122 5 the the DT cord-298078-uqrwq5qk 122 6 mechanical mechanical JJ cord-298078-uqrwq5qk 122 7 strength strength NN cord-298078-uqrwq5qk 122 8 of of IN cord-298078-uqrwq5qk 122 9 the the DT cord-298078-uqrwq5qk 122 10 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 122 11 structure structure NN cord-298078-uqrwq5qk 122 12 is be VBZ cord-298078-uqrwq5qk 122 13 correlated correlate VBN cord-298078-uqrwq5qk 122 14 with with IN cord-298078-uqrwq5qk 122 15 ribosomal ribosomal JJ cord-298078-uqrwq5qk 122 16 frameshifting frameshifting NN cord-298078-uqrwq5qk 122 17 , , , cord-298078-uqrwq5qk 122 18 with with IN cord-298078-uqrwq5qk 122 19 pausing pause VBG cord-298078-uqrwq5qk 122 20 of of IN cord-298078-uqrwq5qk 122 21 ribosome ribosome NN cord-298078-uqrwq5qk 122 22 itself -PRON- PRP cord-298078-uqrwq5qk 122 23 is be VBZ cord-298078-uqrwq5qk 122 24 not not RB cord-298078-uqrwq5qk 122 25 sufficient sufficient JJ cord-298078-uqrwq5qk 122 26 for for IN cord-298078-uqrwq5qk 122 27 frameshifting frameshifte VBG cord-298078-uqrwq5qk 122 28 [ [ -LRB- cord-298078-uqrwq5qk 122 29 33 33 CD cord-298078-uqrwq5qk 122 30 , , , cord-298078-uqrwq5qk 122 31 34 34 CD cord-298078-uqrwq5qk 122 32 , , , cord-298078-uqrwq5qk 122 33 35 35 CD cord-298078-uqrwq5qk 122 34 , , , cord-298078-uqrwq5qk 122 35 36 36 CD cord-298078-uqrwq5qk 122 36 ] ] -RRB- cord-298078-uqrwq5qk 122 37 . . . cord-298078-uqrwq5qk 123 1 While while IN cord-298078-uqrwq5qk 123 2 still still RB cord-298078-uqrwq5qk 123 3 contentious contentious JJ cord-298078-uqrwq5qk 123 4 , , , cord-298078-uqrwq5qk 123 5 several several JJ cord-298078-uqrwq5qk 123 6 reports report NNS cord-298078-uqrwq5qk 123 7 describe describe VBP cord-298078-uqrwq5qk 123 8 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 123 9 binding bind VBG cord-298078-uqrwq5qk 123 10 protein protein NN cord-298078-uqrwq5qk 123 11 factors factor NNS cord-298078-uqrwq5qk 123 12 . . . cord-298078-uqrwq5qk 124 1 For for IN cord-298078-uqrwq5qk 124 2 example example NN cord-298078-uqrwq5qk 124 3 , , , cord-298078-uqrwq5qk 124 4 102-kDa 102-kda CD cord-298078-uqrwq5qk 124 5 RNA RNA NNP cord-298078-uqrwq5qk 124 6 binding bind VBG cord-298078-uqrwq5qk 124 7 protein protein NN cord-298078-uqrwq5qk 124 8 and and CC cord-298078-uqrwq5qk 124 9 eukaryotic eukaryotic JJ cord-298078-uqrwq5qk 124 10 elongation elongation NN cord-298078-uqrwq5qk 124 11 factor factor NN cord-298078-uqrwq5qk 124 12 1 1 CD cord-298078-uqrwq5qk 124 13 ( ( -LRB- cord-298078-uqrwq5qk 124 14 eEF1 eEF1 NNP cord-298078-uqrwq5qk 124 15 ) ) -RRB- cord-298078-uqrwq5qk 124 16 bind bind VB cord-298078-uqrwq5qk 124 17 RNA RNA NNP cord-298078-uqrwq5qk 124 18 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 124 19 of of IN cord-298078-uqrwq5qk 124 20 39-UTR 39-utr CD cord-298078-uqrwq5qk 124 21 in in IN cord-298078-uqrwq5qk 124 22 the the DT cord-298078-uqrwq5qk 124 23 Tobacco Tobacco NNP cord-298078-uqrwq5qk 124 24 Mosaic Mosaic NNP cord-298078-uqrwq5qk 124 25 Virus Virus NNP cord-298078-uqrwq5qk 124 26 genome genome NN cord-298078-uqrwq5qk 124 27 [ [ -LRB- cord-298078-uqrwq5qk 124 28 37 37 CD cord-298078-uqrwq5qk 124 29 , , , cord-298078-uqrwq5qk 124 30 38 38 CD cord-298078-uqrwq5qk 124 31 ] ] -RRB- cord-298078-uqrwq5qk 124 32 . . . cord-298078-uqrwq5qk 125 1 Recently recently RB cord-298078-uqrwq5qk 125 2 , , , cord-298078-uqrwq5qk 125 3 it -PRON- PRP cord-298078-uqrwq5qk 125 4 was be VBD cord-298078-uqrwq5qk 125 5 also also RB cord-298078-uqrwq5qk 125 6 reported report VBN cord-298078-uqrwq5qk 125 7 that that IN cord-298078-uqrwq5qk 125 8 replicase replicase NN cord-298078-uqrwq5qk 125 9 gene gene NN cord-298078-uqrwq5qk 125 10 product product NN cord-298078-uqrwq5qk 125 11 of of IN cord-298078-uqrwq5qk 125 12 mouse mouse NN cord-298078-uqrwq5qk 125 13 coronavirus coronavirus NN cord-298078-uqrwq5qk 125 14 interacts interact VBZ cord-298078-uqrwq5qk 125 15 with with IN cord-298078-uqrwq5qk 125 16 its -PRON- PRP$ cord-298078-uqrwq5qk 125 17 own own JJ cord-298078-uqrwq5qk 125 18 RNA RNA NNP cord-298078-uqrwq5qk 125 19 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 125 20 [ [ -LRB- cord-298078-uqrwq5qk 125 21 29 29 CD cord-298078-uqrwq5qk 125 22 ] ] -RRB- cord-298078-uqrwq5qk 125 23 . . . cord-298078-uqrwq5qk 126 1 Also also RB cord-298078-uqrwq5qk 126 2 , , , cord-298078-uqrwq5qk 126 3 we -PRON- PRP cord-298078-uqrwq5qk 126 4 present present VBP cord-298078-uqrwq5qk 126 5 data datum NNS cord-298078-uqrwq5qk 126 6 showing show VBG cord-298078-uqrwq5qk 126 7 that that IN cord-298078-uqrwq5qk 126 8 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 126 9 ; ; : cord-298078-uqrwq5qk 126 10 Lanes lane NNS cord-298078-uqrwq5qk 126 11 4 4 CD cord-298078-uqrwq5qk 126 12 - - SYM cord-298078-uqrwq5qk 126 13 7 7 CD cord-298078-uqrwq5qk 126 14 contain contain VBP cord-298078-uqrwq5qk 126 15 GST GST NNP cord-298078-uqrwq5qk 126 16 - - HYPH cord-298078-uqrwq5qk 126 17 ANXA2 anxa2 NN cord-298078-uqrwq5qk 126 18 protein protein NN cord-298078-uqrwq5qk 126 19 ( ( -LRB- cord-298078-uqrwq5qk 126 20 0.5 0.5 CD cord-298078-uqrwq5qk 126 21 , , , cord-298078-uqrwq5qk 126 22 2 2 CD cord-298078-uqrwq5qk 126 23 , , , cord-298078-uqrwq5qk 126 24 5 5 CD cord-298078-uqrwq5qk 126 25 and and CC cord-298078-uqrwq5qk 126 26 10 10 CD cord-298078-uqrwq5qk 126 27 mM mm CD cord-298078-uqrwq5qk 126 28 , , , cord-298078-uqrwq5qk 126 29 respectively respectively RB cord-298078-uqrwq5qk 126 30 ) ) -RRB- cord-298078-uqrwq5qk 126 31 . . . cord-298078-uqrwq5qk 127 1 ( ( -LRB- cord-298078-uqrwq5qk 127 2 C C NNP cord-298078-uqrwq5qk 127 3 ) ) -RRB- cord-298078-uqrwq5qk 127 4 RNA rna NN cord-298078-uqrwq5qk 127 5 immunoprecipitation immunoprecipitation NN cord-298078-uqrwq5qk 127 6 assay assay NN cord-298078-uqrwq5qk 127 7 . . . cord-298078-uqrwq5qk 128 1 LNCaP lncap NN cord-298078-uqrwq5qk 128 2 cells cell NNS cord-298078-uqrwq5qk 128 3 were be VBD cord-298078-uqrwq5qk 128 4 cotransfected cotransfecte VBN cord-298078-uqrwq5qk 128 5 with with IN cord-298078-uqrwq5qk 128 6 reporters reporter NNS cord-298078-uqrwq5qk 128 7 ( ( -LRB- cord-298078-uqrwq5qk 128 8 wild wild JJ cord-298078-uqrwq5qk 128 9 - - HYPH cord-298078-uqrwq5qk 128 10 type type NN cord-298078-uqrwq5qk 128 11 or or CC cord-298078-uqrwq5qk 128 12 mutant mutant JJ cord-298078-uqrwq5qk 128 13 IBV IBV NNP cord-298078-uqrwq5qk 128 14 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 128 15 plasmid plasmid VBN cord-298078-uqrwq5qk 128 16 Wild Wild NNP cord-298078-uqrwq5qk 128 17 - - HYPH cord-298078-uqrwq5qk 128 18 type type NN cord-298078-uqrwq5qk 128 19 or or CC cord-298078-uqrwq5qk 128 20 mutant mutant NN cord-298078-uqrwq5qk 128 21 ) ) -RRB- cord-298078-uqrwq5qk 128 22 and and CC cord-298078-uqrwq5qk 128 23 ANXA2 anxa2 IN cord-298078-uqrwq5qk 128 24 expression expression NN cord-298078-uqrwq5qk 128 25 plasmids plasmid NNS cord-298078-uqrwq5qk 128 26 ( ( -LRB- cord-298078-uqrwq5qk 128 27 Flag flag NN cord-298078-uqrwq5qk 128 28 - - HYPH cord-298078-uqrwq5qk 128 29 vector vector NN cord-298078-uqrwq5qk 128 30 or or CC cord-298078-uqrwq5qk 128 31 Flag flag NN cord-298078-uqrwq5qk 128 32 - - HYPH cord-298078-uqrwq5qk 128 33 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 128 34 ) ) -RRB- cord-298078-uqrwq5qk 128 35 . . . cord-298078-uqrwq5qk 129 1 After after IN cord-298078-uqrwq5qk 129 2 formaldehyde formaldehyde NN cord-298078-uqrwq5qk 129 3 fixation fixation NN cord-298078-uqrwq5qk 129 4 , , , cord-298078-uqrwq5qk 129 5 immunoprecipitations immunoprecipitation NNS cord-298078-uqrwq5qk 129 6 were be VBD cord-298078-uqrwq5qk 129 7 performed perform VBN cord-298078-uqrwq5qk 129 8 with with IN cord-298078-uqrwq5qk 129 9 FLAG FLAG NNP cord-298078-uqrwq5qk 129 10 - - HYPH cord-298078-uqrwq5qk 129 11 M2 m2 NN cord-298078-uqrwq5qk 129 12 agarose agarose NN cord-298078-uqrwq5qk 129 13 beads bead NNS cord-298078-uqrwq5qk 129 14 . . . cord-298078-uqrwq5qk 130 1 Bound Bound NNP cord-298078-uqrwq5qk 130 2 RNA RNA NNP cord-298078-uqrwq5qk 130 3 was be VBD cord-298078-uqrwq5qk 130 4 extracted extract VBN cord-298078-uqrwq5qk 130 5 from from IN cord-298078-uqrwq5qk 130 6 the the DT cord-298078-uqrwq5qk 130 7 immune immune JJ cord-298078-uqrwq5qk 130 8 complexes complex NNS cord-298078-uqrwq5qk 130 9 and and CC cord-298078-uqrwq5qk 130 10 analyzed analyze VBN cord-298078-uqrwq5qk 130 11 by by IN cord-298078-uqrwq5qk 130 12 RT RT NNP cord-298078-uqrwq5qk 130 13 - - HYPH cord-298078-uqrwq5qk 130 14 PCR PCR NNP cord-298078-uqrwq5qk 130 15 ( ( -LRB- cord-298078-uqrwq5qk 130 16 left left JJ cord-298078-uqrwq5qk 130 17 panel panel NN cord-298078-uqrwq5qk 130 18 ) ) -RRB- cord-298078-uqrwq5qk 130 19 and and CC cord-298078-uqrwq5qk 130 20 qRT qRT NNP cord-298078-uqrwq5qk 130 21 - - HYPH cord-298078-uqrwq5qk 130 22 PCR PCR NNP cord-298078-uqrwq5qk 130 23 ( ( -LRB- cord-298078-uqrwq5qk 130 24 right right JJ cord-298078-uqrwq5qk 130 25 panel panel NN cord-298078-uqrwq5qk 130 26 ) ) -RRB- cord-298078-uqrwq5qk 130 27 . . . cord-298078-uqrwq5qk 131 1 PCR PCR NNP cord-298078-uqrwq5qk 131 2 products product NNS cord-298078-uqrwq5qk 131 3 were be VBD cord-298078-uqrwq5qk 131 4 resolved resolve VBN cord-298078-uqrwq5qk 131 5 by by IN cord-298078-uqrwq5qk 131 6 electrophoresis electrophoresis NN cord-298078-uqrwq5qk 131 7 in in IN cord-298078-uqrwq5qk 131 8 agarose agarose JJ cord-298078-uqrwq5qk 131 9 gel gel NN cord-298078-uqrwq5qk 131 10 and and CC cord-298078-uqrwq5qk 131 11 visualized visualize VBN cord-298078-uqrwq5qk 131 12 by by IN cord-298078-uqrwq5qk 131 13 staining stain VBG cord-298078-uqrwq5qk 131 14 with with IN cord-298078-uqrwq5qk 131 15 ethidium ethidium NN cord-298078-uqrwq5qk 131 16 bromide bromide NN cord-298078-uqrwq5qk 131 17 . . . cord-298078-uqrwq5qk 132 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 133 1 mRNA mRNA NNP cord-298078-uqrwq5qk 133 2 and and CC cord-298078-uqrwq5qk 133 3 IBV IBV NNP cord-298078-uqrwq5qk 133 4 PK PK NNP cord-298078-uqrwq5qk 133 5 RNA RNA NNP cord-298078-uqrwq5qk 133 6 were be VBD cord-298078-uqrwq5qk 133 7 also also RB cord-298078-uqrwq5qk 133 8 shown show VBN cord-298078-uqrwq5qk 133 9 as as IN cord-298078-uqrwq5qk 133 10 an an DT cord-298078-uqrwq5qk 133 11 expression expression NN cord-298078-uqrwq5qk 133 12 and and CC cord-298078-uqrwq5qk 133 13 input input NN cord-298078-uqrwq5qk 133 14 controls control NNS cord-298078-uqrwq5qk 133 15 . . . cord-298078-uqrwq5qk 134 1 Immunoprecipitation immunoprecipitation NN cord-298078-uqrwq5qk 134 2 of of IN cord-298078-uqrwq5qk 134 3 Flag Flag NNP cord-298078-uqrwq5qk 134 4 - - HYPH cord-298078-uqrwq5qk 134 5 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 134 6 was be VBD cord-298078-uqrwq5qk 134 7 confirmed confirm VBN cord-298078-uqrwq5qk 134 8 by by IN cord-298078-uqrwq5qk 134 9 immunoblotting immunoblotte VBG cord-298078-uqrwq5qk 134 10 ( ( -LRB- cord-298078-uqrwq5qk 134 11 IB IB NNP cord-298078-uqrwq5qk 134 12 ) ) -RRB- cord-298078-uqrwq5qk 134 13 using use VBG cord-298078-uqrwq5qk 134 14 anti anti JJ cord-298078-uqrwq5qk 134 15 - - JJ cord-298078-uqrwq5qk 134 16 Flag flag JJ cord-298078-uqrwq5qk 134 17 antibody antibody NN cord-298078-uqrwq5qk 134 18 . . . cord-298078-uqrwq5qk 135 1 In in IN cord-298078-uqrwq5qk 135 2 the the DT cord-298078-uqrwq5qk 135 3 qRT qRT NNP cord-298078-uqrwq5qk 135 4 - - HYPH cord-298078-uqrwq5qk 135 5 PCR PCR NNP cord-298078-uqrwq5qk 135 6 data datum NNS cord-298078-uqrwq5qk 135 7 , , , cord-298078-uqrwq5qk 135 8 wild wild JJ cord-298078-uqrwq5qk 135 9 - - HYPH cord-298078-uqrwq5qk 135 10 type type NN cord-298078-uqrwq5qk 135 11 PK PK NNP cord-298078-uqrwq5qk 135 12 RNA RNA NNP cord-298078-uqrwq5qk 135 13 ( ( -LRB- cord-298078-uqrwq5qk 135 14 WT WT NNP cord-298078-uqrwq5qk 135 15 ) ) -RRB- cord-298078-uqrwq5qk 135 16 in in IN cord-298078-uqrwq5qk 135 17 binds bind VBZ cord-298078-uqrwq5qk 135 18 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 135 19 RNA RNA NNP cord-298078-uqrwq5qk 135 20 and and CC cord-298078-uqrwq5qk 135 21 inhibits inhibit VBZ cord-298078-uqrwq5qk 135 22 ribosomal ribosomal JJ cord-298078-uqrwq5qk 135 23 frameshifting frameshifting NN cord-298078-uqrwq5qk 135 24 of of IN cord-298078-uqrwq5qk 135 25 IBV IBV NNP cord-298078-uqrwq5qk 135 26 . . . cord-298078-uqrwq5qk 136 1 Even even RB cord-298078-uqrwq5qk 136 2 though though IN cord-298078-uqrwq5qk 136 3 it -PRON- PRP cord-298078-uqrwq5qk 136 4 is be VBZ cord-298078-uqrwq5qk 136 5 necessary necessary JJ cord-298078-uqrwq5qk 136 6 to to TO cord-298078-uqrwq5qk 136 7 determine determine VB cord-298078-uqrwq5qk 136 8 the the DT cord-298078-uqrwq5qk 136 9 exact exact JJ cord-298078-uqrwq5qk 136 10 mode mode NN cord-298078-uqrwq5qk 136 11 of of IN cord-298078-uqrwq5qk 136 12 action action NN cord-298078-uqrwq5qk 136 13 for for IN cord-298078-uqrwq5qk 136 14 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 136 15 on on IN cord-298078-uqrwq5qk 136 16 ribosomal ribosomal JJ cord-298078-uqrwq5qk 136 17 frameshifting frameshifting NN cord-298078-uqrwq5qk 136 18 , , , cord-298078-uqrwq5qk 136 19 evidence evidence NN cord-298078-uqrwq5qk 136 20 is be VBZ cord-298078-uqrwq5qk 136 21 accumulating accumulate VBG cord-298078-uqrwq5qk 136 22 concerning concern VBG cord-298078-uqrwq5qk 136 23 the the DT cord-298078-uqrwq5qk 136 24 role role NN cord-298078-uqrwq5qk 136 25 of of IN cord-298078-uqrwq5qk 136 26 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 136 27 in in IN cord-298078-uqrwq5qk 136 28 translational translational JJ cord-298078-uqrwq5qk 136 29 regulation regulation NN cord-298078-uqrwq5qk 136 30 . . . cord-298078-uqrwq5qk 137 1 For for IN cord-298078-uqrwq5qk 137 2 example example NN cord-298078-uqrwq5qk 137 3 , , , cord-298078-uqrwq5qk 137 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 137 5 is be VBZ cord-298078-uqrwq5qk 137 6 associated associate VBN cord-298078-uqrwq5qk 137 7 with with IN cord-298078-uqrwq5qk 137 8 active active JJ cord-298078-uqrwq5qk 137 9 and and CC cord-298078-uqrwq5qk 137 10 inactive inactive JJ cord-298078-uqrwq5qk 137 11 mRNAs mRNAs NNPS cord-298078-uqrwq5qk 137 12 , , , cord-298078-uqrwq5qk 137 13 polyribosomes polyribosomes NNP cord-298078-uqrwq5qk 137 14 and and CC cord-298078-uqrwq5qk 137 15 many many JJ cord-298078-uqrwq5qk 137 16 ribosomal ribosomal JJ cord-298078-uqrwq5qk 137 17 subunit subunit NN cord-298078-uqrwq5qk 137 18 proteins protein NNS cord-298078-uqrwq5qk 137 19 [ [ -LRB- cord-298078-uqrwq5qk 137 20 12 12 CD cord-298078-uqrwq5qk 137 21 , , , cord-298078-uqrwq5qk 137 22 19 19 CD cord-298078-uqrwq5qk 137 23 ] ] -RRB- cord-298078-uqrwq5qk 137 24 . . . cord-298078-uqrwq5qk 138 1 In in IN cord-298078-uqrwq5qk 138 2 addition addition NN cord-298078-uqrwq5qk 138 3 , , , cord-298078-uqrwq5qk 138 4 the the DT cord-298078-uqrwq5qk 138 5 receptor receptor NN cord-298078-uqrwq5qk 138 6 for for IN cord-298078-uqrwq5qk 138 7 activated activate VBN cord-298078-uqrwq5qk 138 8 C c NN cord-298078-uqrwq5qk 138 9 - - HYPH cord-298078-uqrwq5qk 138 10 kinase kinase NN cord-298078-uqrwq5qk 138 11 ( ( -LRB- cord-298078-uqrwq5qk 138 12 RACK1 rack1 NN cord-298078-uqrwq5qk 138 13 ) ) -RRB- cord-298078-uqrwq5qk 138 14 , , , cord-298078-uqrwq5qk 138 15 which which WDT cord-298078-uqrwq5qk 138 16 resides reside VBZ cord-298078-uqrwq5qk 138 17 in in IN cord-298078-uqrwq5qk 138 18 ribosome ribosome NN cord-298078-uqrwq5qk 138 19 acting act VBG cord-298078-uqrwq5qk 138 20 as as IN cord-298078-uqrwq5qk 138 21 the the DT cord-298078-uqrwq5qk 138 22 signal signal JJ cord-298078-uqrwq5qk 138 23 regulator regulator NN cord-298078-uqrwq5qk 138 24 of of IN cord-298078-uqrwq5qk 138 25 translation translation NN cord-298078-uqrwq5qk 138 26 , , , cord-298078-uqrwq5qk 138 27 also also RB cord-298078-uqrwq5qk 138 28 interacts interact VBZ cord-298078-uqrwq5qk 138 29 with with IN cord-298078-uqrwq5qk 138 30 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 138 31 [ [ -LRB- cord-298078-uqrwq5qk 138 32 39 39 CD cord-298078-uqrwq5qk 138 33 ] ] -RRB- cord-298078-uqrwq5qk 138 34 . . . cord-298078-uqrwq5qk 139 1 Deregulated deregulated JJ cord-298078-uqrwq5qk 139 2 expression expression NN cord-298078-uqrwq5qk 139 3 of of IN cord-298078-uqrwq5qk 139 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 139 5 is be VBZ cord-298078-uqrwq5qk 139 6 implicated implicate VBN cord-298078-uqrwq5qk 139 7 in in IN cord-298078-uqrwq5qk 139 8 many many JJ cord-298078-uqrwq5qk 139 9 human human JJ cord-298078-uqrwq5qk 139 10 tumors tumor NNS cord-298078-uqrwq5qk 139 11 and and CC cord-298078-uqrwq5qk 139 12 diseases disease NNS cord-298078-uqrwq5qk 139 13 [ [ -LRB- cord-298078-uqrwq5qk 139 14 20 20 CD cord-298078-uqrwq5qk 139 15 , , , cord-298078-uqrwq5qk 139 16 40 40 CD cord-298078-uqrwq5qk 139 17 ] ] -RRB- cord-298078-uqrwq5qk 139 18 . . . cord-298078-uqrwq5qk 140 1 Especially especially RB cord-298078-uqrwq5qk 140 2 , , , cord-298078-uqrwq5qk 140 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 140 4 has have VBZ cord-298078-uqrwq5qk 140 5 an an DT cord-298078-uqrwq5qk 140 6 important important JJ cord-298078-uqrwq5qk 140 7 role role NN cord-298078-uqrwq5qk 140 8 in in IN cord-298078-uqrwq5qk 140 9 angiogenesis angiogenesis NN cord-298078-uqrwq5qk 140 10 and and CC cord-298078-uqrwq5qk 140 11 tumor tumor NN cord-298078-uqrwq5qk 140 12 progression progression NN cord-298078-uqrwq5qk 140 13 , , , cord-298078-uqrwq5qk 140 14 and and CC cord-298078-uqrwq5qk 140 15 , , , cord-298078-uqrwq5qk 140 16 thus thus RB cord-298078-uqrwq5qk 140 17 , , , cord-298078-uqrwq5qk 140 18 might may MD cord-298078-uqrwq5qk 140 19 be be VB cord-298078-uqrwq5qk 140 20 a a DT cord-298078-uqrwq5qk 140 21 potential potential JJ cord-298078-uqrwq5qk 140 22 therapeutic therapeutic JJ cord-298078-uqrwq5qk 140 23 target target NN cord-298078-uqrwq5qk 140 24 [ [ -LRB- cord-298078-uqrwq5qk 140 25 41 41 CD cord-298078-uqrwq5qk 140 26 ] ] -RRB- cord-298078-uqrwq5qk 140 27 . . . cord-298078-uqrwq5qk 141 1 Furthermore furthermore RB cord-298078-uqrwq5qk 141 2 , , , cord-298078-uqrwq5qk 141 3 the the DT cord-298078-uqrwq5qk 141 4 gene gene NN cord-298078-uqrwq5qk 141 5 linked link VBN cord-298078-uqrwq5qk 141 6 to to IN cord-298078-uqrwq5qk 141 7 Optiz Optiz NNP cord-298078-uqrwq5qk 141 8 BBB BBB NNP cord-298078-uqrwq5qk 141 9 / / SYM cord-298078-uqrwq5qk 141 10 G g NN cord-298078-uqrwq5qk 141 11 syndrome syndrome NN cord-298078-uqrwq5qk 141 12 , , , cord-298078-uqrwq5qk 141 13 MID1 MID1 NNP cord-298078-uqrwq5qk 141 14 , , , cord-298078-uqrwq5qk 141 15 is be VBZ cord-298078-uqrwq5qk 141 16 associated associate VBN cord-298078-uqrwq5qk 141 17 with with IN cord-298078-uqrwq5qk 141 18 microtubule microtubule NN cord-298078-uqrwq5qk 141 19 - - HYPH cord-298078-uqrwq5qk 141 20 associated associate VBN cord-298078-uqrwq5qk 141 21 ribonucleoprotein ribonucleoprotein NN cord-298078-uqrwq5qk 141 22 complex complex NN cord-298078-uqrwq5qk 141 23 including include VBG cord-298078-uqrwq5qk 141 24 EF-1a ef-1a NN cord-298078-uqrwq5qk 141 25 , , , cord-298078-uqrwq5qk 141 26 RACK-1 rack-1 NN cord-298078-uqrwq5qk 141 27 , , , cord-298078-uqrwq5qk 141 28 small small JJ cord-298078-uqrwq5qk 141 29 ribosomal ribosomal JJ cord-298078-uqrwq5qk 141 30 subunit subunit NN cord-298078-uqrwq5qk 141 31 proteins protein NNS cord-298078-uqrwq5qk 141 32 and and CC cord-298078-uqrwq5qk 141 33 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 141 34 , , , cord-298078-uqrwq5qk 141 35 which which WDT cord-298078-uqrwq5qk 141 36 again again RB cord-298078-uqrwq5qk 141 37 suggests suggest VBZ cord-298078-uqrwq5qk 141 38 a a DT cord-298078-uqrwq5qk 141 39 possible possible JJ cord-298078-uqrwq5qk 141 40 role role NN cord-298078-uqrwq5qk 141 41 of of IN cord-298078-uqrwq5qk 141 42 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 141 43 in in IN cord-298078-uqrwq5qk 141 44 RNA RNA NNP cord-298078-uqrwq5qk 141 45 translation translation NN cord-298078-uqrwq5qk 141 46 and and CC cord-298078-uqrwq5qk 141 47 in in IN cord-298078-uqrwq5qk 141 48 pathogenesis pathogenesis NN cord-298078-uqrwq5qk 141 49 of of IN cord-298078-uqrwq5qk 141 50 the the DT cord-298078-uqrwq5qk 141 51 cell cell NN cord-298078-uqrwq5qk 141 52 [ [ -LRB- cord-298078-uqrwq5qk 141 53 42 42 CD cord-298078-uqrwq5qk 141 54 ] ] -RRB- cord-298078-uqrwq5qk 141 55 . . . cord-298078-uqrwq5qk 142 1 Since since IN cord-298078-uqrwq5qk 142 2 viral viral JJ cord-298078-uqrwq5qk 142 3 frameshifting frameshifting NN cord-298078-uqrwq5qk 142 4 is be VBZ cord-298078-uqrwq5qk 142 5 critical critical JJ cord-298078-uqrwq5qk 142 6 for for IN cord-298078-uqrwq5qk 142 7 many many JJ cord-298078-uqrwq5qk 142 8 pathogenic pathogenic JJ cord-298078-uqrwq5qk 142 9 viruses virus NNS cord-298078-uqrwq5qk 142 10 including include VBG cord-298078-uqrwq5qk 142 11 coronavirus coronavirus NN cord-298078-uqrwq5qk 142 12 , , , cord-298078-uqrwq5qk 142 13 ANXA2-pseudoknot ANXA2-pseudoknot NNP cord-298078-uqrwq5qk 142 14 RNA RNA NNP cord-298078-uqrwq5qk 142 15 interaction interaction NN cord-298078-uqrwq5qk 142 16 might may MD cord-298078-uqrwq5qk 142 17 be be VB cord-298078-uqrwq5qk 142 18 a a DT cord-298078-uqrwq5qk 142 19 novel novel JJ cord-298078-uqrwq5qk 142 20 target target NN cord-298078-uqrwq5qk 142 21 for for IN cord-298078-uqrwq5qk 142 22 anti anti JJ cord-298078-uqrwq5qk 142 23 - - JJ cord-298078-uqrwq5qk 142 24 viral viral JJ cord-298078-uqrwq5qk 142 25 drug drug NN cord-298078-uqrwq5qk 142 26 discovery discovery NN cord-298078-uqrwq5qk 142 27 [ [ -LRB- cord-298078-uqrwq5qk 142 28 43 43 CD cord-298078-uqrwq5qk 142 29 , , , cord-298078-uqrwq5qk 142 30 44 44 CD cord-298078-uqrwq5qk 142 31 ] ] -RRB- cord-298078-uqrwq5qk 142 32 . . . cord-298078-uqrwq5qk 143 1 Plasmids plasmid NNS cord-298078-uqrwq5qk 143 2 containing contain VBG cord-298078-uqrwq5qk 143 3 wild wild JJ cord-298078-uqrwq5qk 143 4 - - HYPH cord-298078-uqrwq5qk 143 5 type type NN cord-298078-uqrwq5qk 143 6 ( ( -LRB- cord-298078-uqrwq5qk 143 7 pFS pf NNS cord-298078-uqrwq5qk 143 8 - - HYPH cord-298078-uqrwq5qk 143 9 cass5 cass5 NN cord-298078-uqrwq5qk 143 10 ) ) -RRB- cord-298078-uqrwq5qk 143 11 and and CC cord-298078-uqrwq5qk 143 12 mutant mutant JJ cord-298078-uqrwq5qk 143 13 ( ( -LRB- cord-298078-uqrwq5qk 143 14 pFS pfs NN cord-298078-uqrwq5qk 143 15 - - HYPH cord-298078-uqrwq5qk 143 16 cass5.15 cass5.15 VB cord-298078-uqrwq5qk 143 17 ) ) -RRB- cord-298078-uqrwq5qk 143 18 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 143 19 sequences sequence NNS cord-298078-uqrwq5qk 143 20 from from IN cord-298078-uqrwq5qk 143 21 IBV IBV NNP cord-298078-uqrwq5qk 143 22 genomic genomic JJ cord-298078-uqrwq5qk 143 23 RNA RNA NNP cord-298078-uqrwq5qk 143 24 were be VBD cord-298078-uqrwq5qk 143 25 generous generous JJ cord-298078-uqrwq5qk 143 26 gifts gift NNS cord-298078-uqrwq5qk 143 27 form form NN cord-298078-uqrwq5qk 143 28 Dr. Dr. NNP cord-298078-uqrwq5qk 143 29 Ian Ian NNP cord-298078-uqrwq5qk 143 30 Brierley Brierley NNP cord-298078-uqrwq5qk 143 31 ( ( -LRB- cord-298078-uqrwq5qk 143 32 University University NNP cord-298078-uqrwq5qk 143 33 of of IN cord-298078-uqrwq5qk 143 34 Cambridge Cambridge NNP cord-298078-uqrwq5qk 143 35 ) ) -RRB- cord-298078-uqrwq5qk 144 1 [ [ -LRB- cord-298078-uqrwq5qk 144 2 3 3 CD cord-298078-uqrwq5qk 144 3 ] ] -RRB- cord-298078-uqrwq5qk 144 4 . . . cord-298078-uqrwq5qk 145 1 Bovine bovine JJ cord-298078-uqrwq5qk 145 2 ANXA2 anxa2 NN cord-298078-uqrwq5qk 145 3 cDNA cdna NN cord-298078-uqrwq5qk 145 4 was be VBD cord-298078-uqrwq5qk 145 5 kindly kindly RB cord-298078-uqrwq5qk 145 6 provided provide VBN cord-298078-uqrwq5qk 145 7 by by IN cord-298078-uqrwq5qk 145 8 Dr. Dr. NNP cord-298078-uqrwq5qk 145 9 Anni Anni NNP cord-298078-uqrwq5qk 145 10 Vedeler Vedeler NNP cord-298078-uqrwq5qk 145 11 ( ( -LRB- cord-298078-uqrwq5qk 145 12 University University NNP cord-298078-uqrwq5qk 145 13 of of IN cord-298078-uqrwq5qk 145 14 Bergen Bergen NNP cord-298078-uqrwq5qk 145 15 ) ) -RRB- cord-298078-uqrwq5qk 146 1 [ [ -LRB- cord-298078-uqrwq5qk 146 2 12 12 CD cord-298078-uqrwq5qk 146 3 ] ] -RRB- cord-298078-uqrwq5qk 146 4 . . . cord-298078-uqrwq5qk 147 1 Human human JJ cord-298078-uqrwq5qk 147 2 embryonic embryonic JJ cord-298078-uqrwq5qk 147 3 kidney kidney NN cord-298078-uqrwq5qk 147 4 cell cell NN cord-298078-uqrwq5qk 147 5 line line NN cord-298078-uqrwq5qk 147 6 HEK293 HEK293 NNP cord-298078-uqrwq5qk 147 7 T T NNP cord-298078-uqrwq5qk 147 8 were be VBD cord-298078-uqrwq5qk 147 9 purchased purchase VBN cord-298078-uqrwq5qk 147 10 from from IN cord-298078-uqrwq5qk 147 11 the the DT cord-298078-uqrwq5qk 147 12 American American NNP cord-298078-uqrwq5qk 147 13 Type Type NNP cord-298078-uqrwq5qk 147 14 Culture Culture NNP cord-298078-uqrwq5qk 147 15 Collection Collection NNP cord-298078-uqrwq5qk 147 16 ( ( -LRB- cord-298078-uqrwq5qk 147 17 ATCC ATCC NNP cord-298078-uqrwq5qk 147 18 ) ) -RRB- cord-298078-uqrwq5qk 147 19 and and CC cord-298078-uqrwq5qk 147 20 maintained maintain VBN cord-298078-uqrwq5qk 147 21 in in IN cord-298078-uqrwq5qk 147 22 Dulbecco Dulbecco NNP cord-298078-uqrwq5qk 147 23 's 's POS cord-298078-uqrwq5qk 147 24 modified modify VBN cord-298078-uqrwq5qk 147 25 Eagle Eagle NNP cord-298078-uqrwq5qk 147 26 's 's POS cord-298078-uqrwq5qk 147 27 medium medium NN cord-298078-uqrwq5qk 147 28 ( ( -LRB- cord-298078-uqrwq5qk 147 29 DMEM DMEM NNP cord-298078-uqrwq5qk 147 30 ) ) -RRB- cord-298078-uqrwq5qk 147 31 containing contain VBG cord-298078-uqrwq5qk 147 32 10 10 CD cord-298078-uqrwq5qk 147 33 % % NN cord-298078-uqrwq5qk 147 34 fetal fetal JJ cord-298078-uqrwq5qk 147 35 bovine bovine JJ cord-298078-uqrwq5qk 147 36 serum serum NN cord-298078-uqrwq5qk 147 37 ( ( -LRB- cord-298078-uqrwq5qk 147 38 FBS FBS NNP cord-298078-uqrwq5qk 147 39 ; ; : cord-298078-uqrwq5qk 147 40 BRL BRL NNP cord-298078-uqrwq5qk 147 41 Life Life NNP cord-298078-uqrwq5qk 147 42 Technology Technology NNP cord-298078-uqrwq5qk 147 43 ) ) -RRB- cord-298078-uqrwq5qk 147 44 . . . cord-298078-uqrwq5qk 148 1 Human human JJ cord-298078-uqrwq5qk 148 2 prostate prostate NN cord-298078-uqrwq5qk 148 3 cancer cancer NN cord-298078-uqrwq5qk 148 4 cell cell NN cord-298078-uqrwq5qk 148 5 line line NN cord-298078-uqrwq5qk 148 6 LNCaP lncap NN cord-298078-uqrwq5qk 148 7 were be VBD cord-298078-uqrwq5qk 148 8 purchased purchase VBN cord-298078-uqrwq5qk 148 9 from from IN cord-298078-uqrwq5qk 148 10 the the DT cord-298078-uqrwq5qk 148 11 ATCC ATCC NNP cord-298078-uqrwq5qk 148 12 and and CC cord-298078-uqrwq5qk 148 13 cultured culture VBN cord-298078-uqrwq5qk 148 14 in in IN cord-298078-uqrwq5qk 148 15 RPMI1640 rpmi1640 NN cord-298078-uqrwq5qk 148 16 with with IN cord-298078-uqrwq5qk 148 17 10 10 CD cord-298078-uqrwq5qk 148 18 % % NN cord-298078-uqrwq5qk 148 19 FBS FBS NNP cord-298078-uqrwq5qk 148 20 . . . cord-298078-uqrwq5qk 149 1 Human human JJ cord-298078-uqrwq5qk 149 2 lung lung NN cord-298078-uqrwq5qk 149 3 fibroblast fibroblast NN cord-298078-uqrwq5qk 149 4 IMR90 IMR90 NNP cord-298078-uqrwq5qk 149 5 and and CC cord-298078-uqrwq5qk 150 1 human human JJ cord-298078-uqrwq5qk 150 2 lung lung NN cord-298078-uqrwq5qk 150 3 adenocarcinoma adenocarcinoma NN cord-298078-uqrwq5qk 150 4 SK SK NNP cord-298078-uqrwq5qk 150 5 - - HYPH cord-298078-uqrwq5qk 150 6 Lu Lu NNP cord-298078-uqrwq5qk 151 1 I -PRON- PRP cord-298078-uqrwq5qk 151 2 cells cell NNS cord-298078-uqrwq5qk 151 3 were be VBD cord-298078-uqrwq5qk 151 4 purchased purchase VBN cord-298078-uqrwq5qk 151 5 from from IN cord-298078-uqrwq5qk 151 6 ATCC ATCC NNP cord-298078-uqrwq5qk 151 7 and and CC cord-298078-uqrwq5qk 151 8 cultured culture VBN cord-298078-uqrwq5qk 151 9 in in IN cord-298078-uqrwq5qk 151 10 MEM MEM NNP cord-298078-uqrwq5qk 151 11 containing contain VBG cord-298078-uqrwq5qk 151 12 10 10 CD cord-298078-uqrwq5qk 151 13 % % NN cord-298078-uqrwq5qk 151 14 FBS FBS NNP cord-298078-uqrwq5qk 151 15 using use VBG cord-298078-uqrwq5qk 151 16 standard standard JJ cord-298078-uqrwq5qk 151 17 techniques technique NNS cord-298078-uqrwq5qk 151 18 . . . cord-298078-uqrwq5qk 152 1 Cells cell NNS cord-298078-uqrwq5qk 152 2 were be VBD cord-298078-uqrwq5qk 152 3 harvested harvest VBN cord-298078-uqrwq5qk 152 4 and and CC cord-298078-uqrwq5qk 152 5 washed wash VBN cord-298078-uqrwq5qk 152 6 twice twice RB cord-298078-uqrwq5qk 152 7 with with IN cord-298078-uqrwq5qk 152 8 ice ice NN cord-298078-uqrwq5qk 152 9 - - HYPH cord-298078-uqrwq5qk 152 10 cold cold JJ cord-298078-uqrwq5qk 152 11 phosphate phosphate NN cord-298078-uqrwq5qk 152 12 buffered buffer VBN cord-298078-uqrwq5qk 152 13 saline saline NN cord-298078-uqrwq5qk 152 14 ( ( -LRB- cord-298078-uqrwq5qk 152 15 PBS PBS NNP cord-298078-uqrwq5qk 152 16 ) ) -RRB- cord-298078-uqrwq5qk 152 17 . . . cord-298078-uqrwq5qk 153 1 Cell cell NN cord-298078-uqrwq5qk 153 2 pellets pellet NNS cord-298078-uqrwq5qk 153 3 were be VBD cord-298078-uqrwq5qk 153 4 resuspended resuspend VBN cord-298078-uqrwq5qk 153 5 in in IN cord-298078-uqrwq5qk 153 6 two two CD cord-298078-uqrwq5qk 153 7 volumes volume NNS cord-298078-uqrwq5qk 153 8 of of IN cord-298078-uqrwq5qk 153 9 buffer buffer NN cord-298078-uqrwq5qk 153 10 A a NN cord-298078-uqrwq5qk 153 11 ( ( -LRB- cord-298078-uqrwq5qk 153 12 20 20 CD cord-298078-uqrwq5qk 153 13 mM mm CD cord-298078-uqrwq5qk 153 14 HEPES HEPES NNP cord-298078-uqrwq5qk 153 15 , , , cord-298078-uqrwq5qk 153 16 pH pH NNP cord-298078-uqrwq5qk 153 17 7.9 7.9 CD cord-298078-uqrwq5qk 153 18 ; ; : cord-298078-uqrwq5qk 153 19 10 10 CD cord-298078-uqrwq5qk 153 20 mM mm CD cord-298078-uqrwq5qk 153 21 NaCl nacl NN cord-298078-uqrwq5qk 153 22 ; ; : cord-298078-uqrwq5qk 153 23 1 1 CD cord-298078-uqrwq5qk 153 24 mM mm CD cord-298078-uqrwq5qk 153 25 EDTA edta NN cord-298078-uqrwq5qk 153 26 ; ; : cord-298078-uqrwq5qk 153 27 1 1 CD cord-298078-uqrwq5qk 153 28 mM mm CD cord-298078-uqrwq5qk 153 29 DTT DTT NNP cord-298078-uqrwq5qk 153 30 ) ) -RRB- cord-298078-uqrwq5qk 153 31 supplemented supplement VBN cord-298078-uqrwq5qk 153 32 with with IN cord-298078-uqrwq5qk 153 33 protein protein NN cord-298078-uqrwq5qk 153 34 inhibitor inhibitor NN cord-298078-uqrwq5qk 153 35 cocktail cocktail NN cord-298078-uqrwq5qk 153 36 ( ( -LRB- cord-298078-uqrwq5qk 153 37 1 1 CD cord-298078-uqrwq5qk 153 38 mM mm CD cord-298078-uqrwq5qk 153 39 PMSF PMSF NNP cord-298078-uqrwq5qk 153 40 , , , cord-298078-uqrwq5qk 153 41 Sigma Sigma NNP cord-298078-uqrwq5qk 153 42 - - HYPH cord-298078-uqrwq5qk 153 43 Aldrich Aldrich NNP cord-298078-uqrwq5qk 153 44 ) ) -RRB- cord-298078-uqrwq5qk 153 45 . . . cord-298078-uqrwq5qk 154 1 After after IN cord-298078-uqrwq5qk 154 2 incubating incubate VBG cord-298078-uqrwq5qk 154 3 15 15 CD cord-298078-uqrwq5qk 154 4 min min NN cord-298078-uqrwq5qk 154 5 on on IN cord-298078-uqrwq5qk 154 6 ice ice NN cord-298078-uqrwq5qk 154 7 , , , cord-298078-uqrwq5qk 154 8 cells cell NNS cord-298078-uqrwq5qk 154 9 were be VBD cord-298078-uqrwq5qk 154 10 lysed lyse VBN cord-298078-uqrwq5qk 154 11 with with IN cord-298078-uqrwq5qk 154 12 0.5 0.5 CD cord-298078-uqrwq5qk 154 13 % % NN cord-298078-uqrwq5qk 154 14 Nonidet Nonidet NNP cord-298078-uqrwq5qk 154 15 P-40 P-40 NNP cord-298078-uqrwq5qk 154 16 and and CC cord-298078-uqrwq5qk 154 17 centrifuged centrifuge VBD cord-298078-uqrwq5qk 154 18 at at IN cord-298078-uqrwq5qk 154 19 4,000 4,000 CD cord-298078-uqrwq5qk 154 20 rpm rpm NN cord-298078-uqrwq5qk 154 21 at at IN cord-298078-uqrwq5qk 154 22 4uC 4uc CD cord-298078-uqrwq5qk 154 23 for for IN cord-298078-uqrwq5qk 154 24 30 30 CD cord-298078-uqrwq5qk 154 25 sec sec NNP cord-298078-uqrwq5qk 154 26 . . . cord-298078-uqrwq5qk 155 1 Clarified clarify VBN cord-298078-uqrwq5qk 155 2 cytosolic cytosolic JJ cord-298078-uqrwq5qk 155 3 extract extract NN cord-298078-uqrwq5qk 155 4 was be VBD cord-298078-uqrwq5qk 155 5 quantitated quantitate VBN cord-298078-uqrwq5qk 155 6 for for IN cord-298078-uqrwq5qk 155 7 protein protein NN cord-298078-uqrwq5qk 155 8 concentration concentration NN cord-298078-uqrwq5qk 155 9 and and CC cord-298078-uqrwq5qk 155 10 stored store VBN cord-298078-uqrwq5qk 155 11 in in IN cord-298078-uqrwq5qk 155 12 aliquots aliquot NNS cord-298078-uqrwq5qk 155 13 at at IN cord-298078-uqrwq5qk 155 14 280uC. 280uc. CD cord-298078-uqrwq5qk 155 15 Wild wild JJ cord-298078-uqrwq5qk 155 16 - - HYPH cord-298078-uqrwq5qk 155 17 type type NN cord-298078-uqrwq5qk 155 18 and and CC cord-298078-uqrwq5qk 155 19 mutant mutant JJ cord-298078-uqrwq5qk 155 20 IBV IBV NNP cord-298078-uqrwq5qk 155 21 RNAs rna NNS cord-298078-uqrwq5qk 155 22 were be VBD cord-298078-uqrwq5qk 155 23 transcribed transcribe VBN cord-298078-uqrwq5qk 155 24 in in IN cord-298078-uqrwq5qk 155 25 the the DT cord-298078-uqrwq5qk 155 26 presence presence NN cord-298078-uqrwq5qk 155 27 of of IN cord-298078-uqrwq5qk 155 28 4-thio 4-thio CD cord-298078-uqrwq5qk 155 29 UTP UTP NNP cord-298078-uqrwq5qk 155 30 ( ( -LRB- cord-298078-uqrwq5qk 155 31 Ambion Ambion NNP cord-298078-uqrwq5qk 155 32 ) ) -RRB- cord-298078-uqrwq5qk 155 33 by by IN cord-298078-uqrwq5qk 155 34 in in IN cord-298078-uqrwq5qk 155 35 vitro vitro FW cord-298078-uqrwq5qk 155 36 transcription transcription NN cord-298078-uqrwq5qk 155 37 with with IN cord-298078-uqrwq5qk 155 38 T7 T7 NNP cord-298078-uqrwq5qk 155 39 RNA RNA NNP cord-298078-uqrwq5qk 155 40 polymerase polymerase NN cord-298078-uqrwq5qk 155 41 . . . cord-298078-uqrwq5qk 156 1 Phosphate phosphate NN cord-298078-uqrwq5qk 156 2 was be VBD cord-298078-uqrwq5qk 156 3 removed remove VBN cord-298078-uqrwq5qk 156 4 from from IN cord-298078-uqrwq5qk 156 5 59-end 59-end CD cord-298078-uqrwq5qk 156 6 by by IN cord-298078-uqrwq5qk 156 7 Calf calf NN cord-298078-uqrwq5qk 156 8 Intestinal intestinal JJ cord-298078-uqrwq5qk 156 9 Alkaline alkaline JJ cord-298078-uqrwq5qk 156 10 Phosphatase Phosphatase NNP cord-298078-uqrwq5qk 156 11 ( ( -LRB- cord-298078-uqrwq5qk 156 12 New New NNP cord-298078-uqrwq5qk 156 13 England England NNP cord-298078-uqrwq5qk 156 14 Bio Bio NNP cord-298078-uqrwq5qk 156 15 Lab Lab NNP cord-298078-uqrwq5qk 156 16 ) ) -RRB- cord-298078-uqrwq5qk 156 17 and and CC cord-298078-uqrwq5qk 156 18 c c LS cord-298078-uqrwq5qk 156 19 - - HYPH cord-298078-uqrwq5qk 156 20 S S NNP cord-298078-uqrwq5qk 156 21 32 32 CD cord-298078-uqrwq5qk 156 22 was be VBD cord-298078-uqrwq5qk 156 23 added add VBN cord-298078-uqrwq5qk 156 24 by by IN cord-298078-uqrwq5qk 156 25 incubating incubate VBG cord-298078-uqrwq5qk 156 26 with with IN cord-298078-uqrwq5qk 156 27 c c NN cord-298078-uqrwq5qk 156 28 - - HYPH cord-298078-uqrwq5qk 156 29 S S NNP cord-298078-uqrwq5qk 156 30 32 32 CD cord-298078-uqrwq5qk 156 31 ATP ATP NNP cord-298078-uqrwq5qk 156 32 ( ( -LRB- cord-298078-uqrwq5qk 156 33 10 10 CD cord-298078-uqrwq5qk 156 34 mM mm CD cord-298078-uqrwq5qk 156 35 ) ) -RRB- cord-298078-uqrwq5qk 156 36 with with IN cord-298078-uqrwq5qk 156 37 T4 T4 NNP cord-298078-uqrwq5qk 156 38 Polynucleotide Polynucleotide NNP cord-298078-uqrwq5qk 156 39 Kinase Kinase NNP cord-298078-uqrwq5qk 156 40 ( ( -LRB- cord-298078-uqrwq5qk 156 41 New New NNP cord-298078-uqrwq5qk 156 42 England England NNP cord-298078-uqrwq5qk 156 43 Bio Bio NNP cord-298078-uqrwq5qk 156 44 Lab Lab NNP cord-298078-uqrwq5qk 156 45 ) ) -RRB- cord-298078-uqrwq5qk 156 46 . . . cord-298078-uqrwq5qk 157 1 Biotin biotin NN cord-298078-uqrwq5qk 157 2 was be VBD cord-298078-uqrwq5qk 157 3 added add VBN cord-298078-uqrwq5qk 157 4 at at IN cord-298078-uqrwq5qk 157 5 the the DT cord-298078-uqrwq5qk 157 6 59-end 59-end CD cord-298078-uqrwq5qk 157 7 of of IN cord-298078-uqrwq5qk 157 8 the the DT cord-298078-uqrwq5qk 157 9 prepared prepared JJ cord-298078-uqrwq5qk 157 10 RNA RNA NNP cord-298078-uqrwq5qk 157 11 by by IN cord-298078-uqrwq5qk 157 12 incubating incubate VBG cord-298078-uqrwq5qk 157 13 one one CD cord-298078-uqrwq5qk 157 14 and and CC cord-298078-uqrwq5qk 157 15 half half NN cord-298078-uqrwq5qk 157 16 hours hour NNS cord-298078-uqrwq5qk 157 17 with with IN cord-298078-uqrwq5qk 157 18 10 10 CD cord-298078-uqrwq5qk 157 19 mM mm CD cord-298078-uqrwq5qk 157 20 PEO PEO NNP cord-298078-uqrwq5qk 157 21 - - HYPH cord-298078-uqrwq5qk 157 22 Iodoacetyl Iodoacetyl NNP cord-298078-uqrwq5qk 157 23 Biotin Biotin NNP cord-298078-uqrwq5qk 157 24 ( ( -LRB- cord-298078-uqrwq5qk 157 25 PIERCE PIERCE NNP cord-298078-uqrwq5qk 157 26 ) ) -RRB- cord-298078-uqrwq5qk 157 27 in in IN cord-298078-uqrwq5qk 157 28 the the DT cord-298078-uqrwq5qk 157 29 dark dark NN cord-298078-uqrwq5qk 157 30 . . . cord-298078-uqrwq5qk 158 1 Cytosolic cytosolic JJ cord-298078-uqrwq5qk 158 2 extracts extract NNS cord-298078-uqrwq5qk 158 3 were be VBD cord-298078-uqrwq5qk 158 4 pre pre JJ cord-298078-uqrwq5qk 158 5 - - VBN cord-298078-uqrwq5qk 158 6 cleared clear VBN cord-298078-uqrwq5qk 159 1 with with IN cord-298078-uqrwq5qk 159 2 Streptavidin Streptavidin NNP cord-298078-uqrwq5qk 159 3 - - HYPH cord-298078-uqrwq5qk 159 4 coated coat VBN cord-298078-uqrwq5qk 159 5 magnetic magnetic JJ cord-298078-uqrwq5qk 159 6 beads bead NNS cord-298078-uqrwq5qk 159 7 ( ( -LRB- cord-298078-uqrwq5qk 159 8 New New NNP cord-298078-uqrwq5qk 159 9 England England NNP cord-298078-uqrwq5qk 159 10 Bio Bio NNP cord-298078-uqrwq5qk 159 11 Lab Lab NNP cord-298078-uqrwq5qk 159 12 ) ) -RRB- cord-298078-uqrwq5qk 160 1 and and CC cord-298078-uqrwq5qk 160 2 beads bead NNS cord-298078-uqrwq5qk 160 3 were be VBD cord-298078-uqrwq5qk 160 4 preblocked preblocke VBN cord-298078-uqrwq5qk 160 5 with with IN cord-298078-uqrwq5qk 160 6 tRNA tRNA NNP cord-298078-uqrwq5qk 160 7 . . . cord-298078-uqrwq5qk 161 1 Biotin biotin NN cord-298078-uqrwq5qk 161 2 and and CC cord-298078-uqrwq5qk 161 3 4-thio 4-thio CD cord-298078-uqrwq5qk 161 4 labeled label VBN cord-298078-uqrwq5qk 161 5 RNA RNA NNP cord-298078-uqrwq5qk 161 6 was be VBD cord-298078-uqrwq5qk 161 7 incubated incubate VBN cord-298078-uqrwq5qk 161 8 with with IN cord-298078-uqrwq5qk 161 9 pre pre JJ cord-298078-uqrwq5qk 161 10 - - JJ cord-298078-uqrwq5qk 161 11 cleared cleared JJ cord-298078-uqrwq5qk 161 12 cell cell NN cord-298078-uqrwq5qk 161 13 extract extract NN cord-298078-uqrwq5qk 161 14 in in IN cord-298078-uqrwq5qk 161 15 binding bind VBG cord-298078-uqrwq5qk 161 16 buffer buffer NN cord-298078-uqrwq5qk 161 17 ( ( -LRB- cord-298078-uqrwq5qk 161 18 20 20 CD cord-298078-uqrwq5qk 161 19 mM mm CD cord-298078-uqrwq5qk 161 20 HEPES HEPES NNP cord-298078-uqrwq5qk 161 21 , , , cord-298078-uqrwq5qk 161 22 pH pH NNP cord-298078-uqrwq5qk 161 23 7.5 7.5 CD cord-298078-uqrwq5qk 161 24 ; ; : cord-298078-uqrwq5qk 161 25 50 50 CD cord-298078-uqrwq5qk 161 26 mM mm CD cord-298078-uqrwq5qk 161 27 KCl KCl NNS cord-298078-uqrwq5qk 161 28 ; ; : cord-298078-uqrwq5qk 161 29 1 1 CD cord-298078-uqrwq5qk 161 30 mM mm CD cord-298078-uqrwq5qk 161 31 DTT dtt NN cord-298078-uqrwq5qk 161 32 ; ; : cord-298078-uqrwq5qk 161 33 0.1 0.1 CD cord-298078-uqrwq5qk 161 34 mM mm CD cord-298078-uqrwq5qk 161 35 EDTA edta NN cord-298078-uqrwq5qk 161 36 ; ; : cord-298078-uqrwq5qk 161 37 5 5 CD cord-298078-uqrwq5qk 161 38 % % NN cord-298078-uqrwq5qk 161 39 glycerol glycerol NN cord-298078-uqrwq5qk 161 40 ; ; : cord-298078-uqrwq5qk 161 41 40 40 CD cord-298078-uqrwq5qk 161 42 U u NN cord-298078-uqrwq5qk 161 43 RNase rnase NN cord-298078-uqrwq5qk 161 44 inhibitor inhibitor NN cord-298078-uqrwq5qk 161 45 ) ) -RRB- cord-298078-uqrwq5qk 161 46 for for IN cord-298078-uqrwq5qk 161 47 one one CD cord-298078-uqrwq5qk 161 48 hour hour NN cord-298078-uqrwq5qk 161 49 in in IN cord-298078-uqrwq5qk 161 50 room room NN cord-298078-uqrwq5qk 161 51 temperature temperature NN cord-298078-uqrwq5qk 161 52 . . . cord-298078-uqrwq5qk 162 1 Ultraviolet ultraviolet NN cord-298078-uqrwq5qk 162 2 radiation radiation NN cord-298078-uqrwq5qk 162 3 ( ( -LRB- cord-298078-uqrwq5qk 162 4 365 365 CD cord-298078-uqrwq5qk 162 5 nm nm NN cord-298078-uqrwq5qk 162 6 ) ) -RRB- cord-298078-uqrwq5qk 162 7 was be VBD cord-298078-uqrwq5qk 162 8 performed perform VBN cord-298078-uqrwq5qk 162 9 to to TO cord-298078-uqrwq5qk 162 10 induce induce VB cord-298078-uqrwq5qk 162 11 binding bind VBG cord-298078-uqrwq5qk 162 12 between between IN cord-298078-uqrwq5qk 162 13 4-thio 4-thio CD cord-298078-uqrwq5qk 162 14 UTP UTP NNP cord-298078-uqrwq5qk 162 15 and and CC cord-298078-uqrwq5qk 162 16 interacting interact VBG cord-298078-uqrwq5qk 162 17 proteins protein NNS cord-298078-uqrwq5qk 162 18 . . . cord-298078-uqrwq5qk 163 1 Pre pre JJ cord-298078-uqrwq5qk 163 2 - - JJ cord-298078-uqrwq5qk 163 3 blocked block VBN cord-298078-uqrwq5qk 163 4 magnetic magnetic JJ cord-298078-uqrwq5qk 163 5 beads bead NNS cord-298078-uqrwq5qk 163 6 were be VBD cord-298078-uqrwq5qk 163 7 added add VBN cord-298078-uqrwq5qk 163 8 and and CC cord-298078-uqrwq5qk 163 9 incubated incubate VBN cord-298078-uqrwq5qk 163 10 for for IN cord-298078-uqrwq5qk 163 11 one one CD cord-298078-uqrwq5qk 163 12 hour hour NN cord-298078-uqrwq5qk 163 13 to to IN cord-298078-uqrwq5qk 163 14 pellet pellet NN cord-298078-uqrwq5qk 163 15 RNA RNA NNP cord-298078-uqrwq5qk 163 16 - - HYPH cord-298078-uqrwq5qk 163 17 protein protein NN cord-298078-uqrwq5qk 163 18 complexes complex NNS cord-298078-uqrwq5qk 163 19 . . . cord-298078-uqrwq5qk 164 1 Two two CD cord-298078-uqrwq5qk 164 2 volumes volume NNS cord-298078-uqrwq5qk 164 3 of of IN cord-298078-uqrwq5qk 164 4 washing washing NN cord-298078-uqrwq5qk 164 5 buffer buffer NN cord-298078-uqrwq5qk 164 6 ( ( -LRB- cord-298078-uqrwq5qk 164 7 20 20 CD cord-298078-uqrwq5qk 164 8 mM mm CD cord-298078-uqrwq5qk 164 9 HEPES HEPES NNP cord-298078-uqrwq5qk 164 10 , , , cord-298078-uqrwq5qk 164 11 pH pH NNP cord-298078-uqrwq5qk 164 12 7.5 7.5 CD cord-298078-uqrwq5qk 164 13 ; ; : cord-298078-uqrwq5qk 164 14 500 500 CD cord-298078-uqrwq5qk 164 15 mM mm CD cord-298078-uqrwq5qk 164 16 NaCl nacl NN cord-298078-uqrwq5qk 164 17 , , , cord-298078-uqrwq5qk 164 18 1 1 CD cord-298078-uqrwq5qk 164 19 mM mm CD cord-298078-uqrwq5qk 164 20 DTT DTT NNP cord-298078-uqrwq5qk 164 21 , , , cord-298078-uqrwq5qk 164 22 0.1 0.1 CD cord-298078-uqrwq5qk 164 23 mM mm CD cord-298078-uqrwq5qk 164 24 EDTA EDTA NNP cord-298078-uqrwq5qk 164 25 , , , cord-298078-uqrwq5qk 164 26 5 5 CD cord-298078-uqrwq5qk 164 27 % % NN cord-298078-uqrwq5qk 164 28 glycerol glycerol NN cord-298078-uqrwq5qk 164 29 ) ) -RRB- cord-298078-uqrwq5qk 164 30 was be VBD cord-298078-uqrwq5qk 164 31 added add VBN cord-298078-uqrwq5qk 164 32 and and CC cord-298078-uqrwq5qk 164 33 washed wash VBD cord-298078-uqrwq5qk 164 34 seven seven CD cord-298078-uqrwq5qk 164 35 times time NNS cord-298078-uqrwq5qk 164 36 , , , cord-298078-uqrwq5qk 164 37 followed follow VBN cord-298078-uqrwq5qk 164 38 by by IN cord-298078-uqrwq5qk 164 39 elution elution NN cord-298078-uqrwq5qk 164 40 with with IN cord-298078-uqrwq5qk 164 41 boiling boil VBG cord-298078-uqrwq5qk 164 42 in in IN cord-298078-uqrwq5qk 164 43 26 26 CD cord-298078-uqrwq5qk 164 44 sample sample NN cord-298078-uqrwq5qk 164 45 buffer buffer NN cord-298078-uqrwq5qk 164 46 for for IN cord-298078-uqrwq5qk 164 47 5 5 CD cord-298078-uqrwq5qk 164 48 min min NN cord-298078-uqrwq5qk 164 49 . . . cord-298078-uqrwq5qk 165 1 Proteins protein NNS cord-298078-uqrwq5qk 165 2 were be VBD cord-298078-uqrwq5qk 165 3 separated separate VBN cord-298078-uqrwq5qk 165 4 by by IN cord-298078-uqrwq5qk 165 5 10 10 CD cord-298078-uqrwq5qk 165 6 % % NN cord-298078-uqrwq5qk 165 7 SDS SDS NNP cord-298078-uqrwq5qk 165 8 - - HYPH cord-298078-uqrwq5qk 165 9 PAGE PAGE NNP cord-298078-uqrwq5qk 165 10 and and CC cord-298078-uqrwq5qk 165 11 visualized visualize VBN cord-298078-uqrwq5qk 165 12 by by IN cord-298078-uqrwq5qk 165 13 silver silver JJ cord-298078-uqrwq5qk 165 14 staining staining NN cord-298078-uqrwq5qk 165 15 . . . cord-298078-uqrwq5qk 166 1 After after IN cord-298078-uqrwq5qk 166 2 pulling pull VBG cord-298078-uqrwq5qk 166 3 - - HYPH cord-298078-uqrwq5qk 166 4 down down RP cord-298078-uqrwq5qk 166 5 interacting interact VBG cord-298078-uqrwq5qk 166 6 proteins protein NNS cord-298078-uqrwq5qk 166 7 by by IN cord-298078-uqrwq5qk 166 8 a a DT cord-298078-uqrwq5qk 166 9 biotin biotin NN cord-298078-uqrwq5qk 166 10 pull pull VB cord-298078-uqrwq5qk 166 11 - - HYPH cord-298078-uqrwq5qk 166 12 down down RP cord-298078-uqrwq5qk 166 13 assay assay NN cord-298078-uqrwq5qk 166 14 as as IN cord-298078-uqrwq5qk 166 15 described describe VBN cord-298078-uqrwq5qk 166 16 below below RB cord-298078-uqrwq5qk 166 17 , , , cord-298078-uqrwq5qk 166 18 protein protein NN cord-298078-uqrwq5qk 166 19 identification identification NN cord-298078-uqrwq5qk 166 20 was be VBD cord-298078-uqrwq5qk 166 21 performed perform VBN cord-298078-uqrwq5qk 166 22 in in IN cord-298078-uqrwq5qk 166 23 the the DT cord-298078-uqrwq5qk 166 24 Functional Functional NNP cord-298078-uqrwq5qk 166 25 Proteomics Proteomics NNP cord-298078-uqrwq5qk 166 26 Center Center NNP cord-298078-uqrwq5qk 166 27 , , , cord-298078-uqrwq5qk 166 28 Korea Korea NNP cord-298078-uqrwq5qk 166 29 Institute Institute NNP cord-298078-uqrwq5qk 166 30 of of IN cord-298078-uqrwq5qk 166 31 Science Science NNP cord-298078-uqrwq5qk 166 32 and and CC cord-298078-uqrwq5qk 166 33 Technology Technology NNP cord-298078-uqrwq5qk 166 34 . . . cord-298078-uqrwq5qk 167 1 Silver silver NN cord-298078-uqrwq5qk 167 2 stained stain VBN cord-298078-uqrwq5qk 167 3 bands band NNS cord-298078-uqrwq5qk 167 4 were be VBD cord-298078-uqrwq5qk 167 5 excised excise VBN cord-298078-uqrwq5qk 167 6 and and CC cord-298078-uqrwq5qk 167 7 an an DT cord-298078-uqrwq5qk 167 8 automated automate VBN cord-298078-uqrwq5qk 167 9 in in IN cord-298078-uqrwq5qk 167 10 - - HYPH cord-298078-uqrwq5qk 167 11 gel gel NN cord-298078-uqrwq5qk 167 12 tryptic tryptic JJ cord-298078-uqrwq5qk 167 13 digestion digestion NN cord-298078-uqrwq5qk 167 14 was be VBD cord-298078-uqrwq5qk 167 15 performed perform VBN cord-298078-uqrwq5qk 167 16 on on IN cord-298078-uqrwq5qk 167 17 a a DT cord-298078-uqrwq5qk 167 18 Mass Mass NNP cord-298078-uqrwq5qk 167 19 Prep Prep NNP cord-298078-uqrwq5qk 167 20 Station Station NNP cord-298078-uqrwq5qk 167 21 ( ( -LRB- cord-298078-uqrwq5qk 167 22 Micromass Micromass NNP cord-298078-uqrwq5qk 167 23 ) ) -RRB- cord-298078-uqrwq5qk 167 24 . . . cord-298078-uqrwq5qk 168 1 The the DT cord-298078-uqrwq5qk 168 2 gel gel NN cord-298078-uqrwq5qk 168 3 pieces piece NNS cord-298078-uqrwq5qk 168 4 were be VBD cord-298078-uqrwq5qk 168 5 destained destain VBN cord-298078-uqrwq5qk 168 6 , , , cord-298078-uqrwq5qk 168 7 reduced reduce VBN cord-298078-uqrwq5qk 168 8 with with IN cord-298078-uqrwq5qk 168 9 DTT DTT NNP cord-298078-uqrwq5qk 168 10 , , , cord-298078-uqrwq5qk 168 11 alkylated alkylate VBN cord-298078-uqrwq5qk 168 12 with with IN cord-298078-uqrwq5qk 168 13 iodoacetamide iodoacetamide NN cord-298078-uqrwq5qk 168 14 and and CC cord-298078-uqrwq5qk 168 15 digested digest VBN cord-298078-uqrwq5qk 168 16 with with IN cord-298078-uqrwq5qk 168 17 sequencing sequence VBG cord-298078-uqrwq5qk 168 18 grade grade NN cord-298078-uqrwq5qk 168 19 modified modify VBN cord-298078-uqrwq5qk 168 20 trypsin trypsin NN cord-298078-uqrwq5qk 168 21 ( ( -LRB- cord-298078-uqrwq5qk 168 22 Promega Promega NNP cord-298078-uqrwq5qk 168 23 ) ) -RRB- cord-298078-uqrwq5qk 168 24 . . . cord-298078-uqrwq5qk 169 1 Resulting result VBG cord-298078-uqrwq5qk 169 2 peptides peptide NNS cord-298078-uqrwq5qk 169 3 were be VBD cord-298078-uqrwq5qk 169 4 extracted extract VBN cord-298078-uqrwq5qk 169 5 from from IN cord-298078-uqrwq5qk 169 6 the the DT cord-298078-uqrwq5qk 169 7 gel gel NN cord-298078-uqrwq5qk 169 8 and and CC cord-298078-uqrwq5qk 169 9 analyzed analyze VBD cord-298078-uqrwq5qk 169 10 via via IN cord-298078-uqrwq5qk 169 11 MALDI MALDI NNP cord-298078-uqrwq5qk 169 12 - - HYPH cord-298078-uqrwq5qk 169 13 TOF TOF NNP cord-298078-uqrwq5qk 169 14 and and CC cord-298078-uqrwq5qk 169 15 MALDI MALDI NNP cord-298078-uqrwq5qk 169 16 - - HYPH cord-298078-uqrwq5qk 169 17 TOF TOF NNP cord-298078-uqrwq5qk 169 18 - - HYPH cord-298078-uqrwq5qk 169 19 TOF TOF NNP cord-298078-uqrwq5qk 169 20 . . . cord-298078-uqrwq5qk 170 1 Proteins protein NNS cord-298078-uqrwq5qk 170 2 were be VBD cord-298078-uqrwq5qk 170 3 identified identify VBN cord-298078-uqrwq5qk 170 4 from from IN cord-298078-uqrwq5qk 170 5 the the DT cord-298078-uqrwq5qk 170 6 mass mass NN cord-298078-uqrwq5qk 170 7 spectrometry spectrometry NN cord-298078-uqrwq5qk 170 8 data datum NNS cord-298078-uqrwq5qk 170 9 using use VBG cord-298078-uqrwq5qk 170 10 MASCOT mascot NN cord-298078-uqrwq5qk 170 11 ( ( -LRB- cord-298078-uqrwq5qk 170 12 Matrix Matrix NNP cord-298078-uqrwq5qk 170 13 Science Science NNP cord-298078-uqrwq5qk 170 14 ) ) -RRB- cord-298078-uqrwq5qk 170 15 and and CC cord-298078-uqrwq5qk 170 16 searching search VBG cord-298078-uqrwq5qk 170 17 the the DT cord-298078-uqrwq5qk 170 18 NCBI NCBI NNP cord-298078-uqrwq5qk 170 19 data data NN cord-298078-uqrwq5qk 170 20 base base NN cord-298078-uqrwq5qk 170 21 . . . cord-298078-uqrwq5qk 171 1 IBV IBV NNP cord-298078-uqrwq5qk 171 2 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 171 3 RNA RNA NNP cord-298078-uqrwq5qk 171 4 was be VBD cord-298078-uqrwq5qk 171 5 transcribed transcribe VBN cord-298078-uqrwq5qk 171 6 and and CC cord-298078-uqrwq5qk 171 7 labeled label VBN cord-298078-uqrwq5qk 171 8 in in IN cord-298078-uqrwq5qk 171 9 vitro vitro FW cord-298078-uqrwq5qk 171 10 with with IN cord-298078-uqrwq5qk 171 11 T7 T7 NNP cord-298078-uqrwq5qk 171 12 RNA RNA NNP cord-298078-uqrwq5qk 171 13 polymerase polymerase NN cord-298078-uqrwq5qk 171 14 ( ( -LRB- cord-298078-uqrwq5qk 171 15 Ambion Ambion NNP cord-298078-uqrwq5qk 171 16 ) ) -RRB- cord-298078-uqrwq5qk 171 17 with with IN cord-298078-uqrwq5qk 171 18 a- a- XX cord-298078-uqrwq5qk 171 19 [ [ -LRB- cord-298078-uqrwq5qk 171 20 32 32 CD cord-298078-uqrwq5qk 171 21 P]CTP P]CTP NNP cord-298078-uqrwq5qk 171 22 . . . cord-298078-uqrwq5qk 172 1 32 32 CD cord-298078-uqrwq5qk 173 1 P p NN cord-298078-uqrwq5qk 173 2 - - HYPH cord-298078-uqrwq5qk 173 3 labeled label VBN cord-298078-uqrwq5qk 173 4 IBV IBV NNP cord-298078-uqrwq5qk 173 5 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 173 6 RNA RNA NNP cord-298078-uqrwq5qk 173 7 ( ( -LRB- cord-298078-uqrwq5qk 173 8 1.1610 1.1610 CD cord-298078-uqrwq5qk 173 9 5 5 CD cord-298078-uqrwq5qk 173 10 c.p.m c.p.m JJ cord-298078-uqrwq5qk 173 11 ) ) -RRB- cord-298078-uqrwq5qk 173 12 was be VBD cord-298078-uqrwq5qk 173 13 incubated incubate VBN cord-298078-uqrwq5qk 173 14 with with IN cord-298078-uqrwq5qk 173 15 30 30 CD cord-298078-uqrwq5qk 173 16 mg mg NN cord-298078-uqrwq5qk 173 17 of of IN cord-298078-uqrwq5qk 173 18 cell cell NN cord-298078-uqrwq5qk 173 19 extract extract NN cord-298078-uqrwq5qk 173 20 with with IN cord-298078-uqrwq5qk 173 21 2 2 CD cord-298078-uqrwq5qk 173 22 mg mg NNP cord-298078-uqrwq5qk 173 23 of of IN cord-298078-uqrwq5qk 173 24 tRNA tRNA NNP cord-298078-uqrwq5qk 173 25 , , , cord-298078-uqrwq5qk 173 26 0.2 0.2 CD cord-298078-uqrwq5qk 173 27 mg mg CD cord-298078-uqrwq5qk 173 28 of of IN cord-298078-uqrwq5qk 173 29 poly poly NNP cord-298078-uqrwq5qk 173 30 d(I d(I NNP cord-298078-uqrwq5qk 173 31 - - HYPH cord-298078-uqrwq5qk 173 32 C C NNP cord-298078-uqrwq5qk 173 33 ) ) -RRB- cord-298078-uqrwq5qk 173 34 and and CC cord-298078-uqrwq5qk 173 35 40 40 CD cord-298078-uqrwq5qk 173 36 U U NNS cord-298078-uqrwq5qk 173 37 of of IN cord-298078-uqrwq5qk 173 38 RNase rnase NN cord-298078-uqrwq5qk 173 39 inhibitor inhibitor NN cord-298078-uqrwq5qk 173 40 . . . cord-298078-uqrwq5qk 174 1 The the DT cord-298078-uqrwq5qk 174 2 RNA RNA NNP cord-298078-uqrwq5qk 174 3 - - HYPH cord-298078-uqrwq5qk 174 4 protein protein NN cord-298078-uqrwq5qk 174 5 binding binding NN cord-298078-uqrwq5qk 174 6 reaction reaction NN cord-298078-uqrwq5qk 174 7 was be VBD cord-298078-uqrwq5qk 174 8 carried carry VBN cord-298078-uqrwq5qk 174 9 out out RP cord-298078-uqrwq5qk 174 10 in in IN cord-298078-uqrwq5qk 174 11 a a DT cord-298078-uqrwq5qk 174 12 30 30 CD cord-298078-uqrwq5qk 174 13 ml ml NN cord-298078-uqrwq5qk 174 14 reaction reaction NN cord-298078-uqrwq5qk 174 15 mixture mixture NN cord-298078-uqrwq5qk 174 16 containing contain VBG cord-298078-uqrwq5qk 174 17 20 20 CD cord-298078-uqrwq5qk 174 18 mM mm CD cord-298078-uqrwq5qk 174 19 HEPES HEPES NNP cord-298078-uqrwq5qk 174 20 pH ph NN cord-298078-uqrwq5qk 174 21 7.5 7.5 CD cord-298078-uqrwq5qk 174 22 , , , cord-298078-uqrwq5qk 174 23 50 50 CD cord-298078-uqrwq5qk 174 24 mM mm CD cord-298078-uqrwq5qk 174 25 KCl KCl NNS cord-298078-uqrwq5qk 174 26 , , , cord-298078-uqrwq5qk 174 27 1 1 CD cord-298078-uqrwq5qk 174 28 mM mm CD cord-298078-uqrwq5qk 174 29 DTT DTT NNP cord-298078-uqrwq5qk 174 30 , , , cord-298078-uqrwq5qk 174 31 0.1 0.1 CD cord-298078-uqrwq5qk 174 32 mM mm CD cord-298078-uqrwq5qk 174 33 EDTA edta NN cord-298078-uqrwq5qk 174 34 and and CC cord-298078-uqrwq5qk 174 35 5 5 CD cord-298078-uqrwq5qk 174 36 % % NN cord-298078-uqrwq5qk 174 37 glycerol glycerol NN cord-298078-uqrwq5qk 174 38 . . . cord-298078-uqrwq5qk 175 1 The the DT cord-298078-uqrwq5qk 175 2 mixtures mixture NNS cord-298078-uqrwq5qk 175 3 were be VBD cord-298078-uqrwq5qk 175 4 incubated incubate VBN cord-298078-uqrwq5qk 175 5 at at IN cord-298078-uqrwq5qk 175 6 room room NN cord-298078-uqrwq5qk 175 7 temperature temperature NN cord-298078-uqrwq5qk 175 8 for for IN cord-298078-uqrwq5qk 175 9 30 30 CD cord-298078-uqrwq5qk 175 10 min min NN cord-298078-uqrwq5qk 175 11 , , , cord-298078-uqrwq5qk 175 12 after after IN cord-298078-uqrwq5qk 175 13 which which WDT cord-298078-uqrwq5qk 175 14 they -PRON- PRP cord-298078-uqrwq5qk 175 15 were be VBD cord-298078-uqrwq5qk 175 16 UV uv NN cord-298078-uqrwq5qk 175 17 - - HYPH cord-298078-uqrwq5qk 175 18 irradiated irradiate VBN cord-298078-uqrwq5qk 175 19 ( ( -LRB- cord-298078-uqrwq5qk 175 20 120 120 CD cord-298078-uqrwq5qk 175 21 mJ mJ NNP cord-298078-uqrwq5qk 175 22 / / SYM cord-298078-uqrwq5qk 175 23 cm cm NNP cord-298078-uqrwq5qk 175 24 2 2 CD cord-298078-uqrwq5qk 175 25 ) ) -RRB- cord-298078-uqrwq5qk 175 26 on on IN cord-298078-uqrwq5qk 175 27 ice ice NN cord-298078-uqrwq5qk 175 28 for for IN cord-298078-uqrwq5qk 175 29 30 30 CD cord-298078-uqrwq5qk 175 30 min min NN cord-298078-uqrwq5qk 175 31 with with IN cord-298078-uqrwq5qk 175 32 an an DT cord-298078-uqrwq5qk 175 33 UV uv NN cord-298078-uqrwq5qk 175 34 cross cross NN cord-298078-uqrwq5qk 175 35 linker linker NN cord-298078-uqrwq5qk 175 36 ( ( -LRB- cord-298078-uqrwq5qk 175 37 Fisher Fisher NNP cord-298078-uqrwq5qk 175 38 Scientific Scientific NNP cord-298078-uqrwq5qk 175 39 ) ) -RRB- cord-298078-uqrwq5qk 175 40 . . . cord-298078-uqrwq5qk 176 1 RNAs rna NNS cord-298078-uqrwq5qk 176 2 were be VBD cord-298078-uqrwq5qk 176 3 digested digest VBN cord-298078-uqrwq5qk 176 4 with with IN cord-298078-uqrwq5qk 176 5 1 1 CD cord-298078-uqrwq5qk 176 6 mg mg NN cord-298078-uqrwq5qk 176 7 RNase RNase NNP cord-298078-uqrwq5qk 176 8 A A NNP cord-298078-uqrwq5qk 176 9 , , , cord-298078-uqrwq5qk 176 10 25 25 CD cord-298078-uqrwq5qk 176 11 U U NNP cord-298078-uqrwq5qk 176 12 RNase RNase NNP cord-298078-uqrwq5qk 176 13 S1 s1 NN cord-298078-uqrwq5qk 176 14 and and CC cord-298078-uqrwq5qk 176 15 0.1 0.1 CD cord-298078-uqrwq5qk 176 16 U u NN cord-298078-uqrwq5qk 176 17 RNase rnase NN cord-298078-uqrwq5qk 176 18 V1 V1 VBZ cord-298078-uqrwq5qk 176 19 at at IN cord-298078-uqrwq5qk 176 20 37uC 37uc CD cord-298078-uqrwq5qk 176 21 for for IN cord-298078-uqrwq5qk 176 22 10 10 CD cord-298078-uqrwq5qk 176 23 min min NN cord-298078-uqrwq5qk 176 24 and and CC cord-298078-uqrwq5qk 176 25 analyzed analyze VBN cord-298078-uqrwq5qk 176 26 by by IN cord-298078-uqrwq5qk 176 27 10 10 CD cord-298078-uqrwq5qk 176 28 % % NN cord-298078-uqrwq5qk 176 29 SDS sds NN cord-298078-uqrwq5qk 176 30 - - HYPH cord-298078-uqrwq5qk 176 31 PAGE PAGE NNP cord-298078-uqrwq5qk 176 32 . . . cord-298078-uqrwq5qk 177 1 Anti anti JJ cord-298078-uqrwq5qk 177 2 - - JJ cord-298078-uqrwq5qk 177 3 ANXA2 anxa2 JJ cord-298078-uqrwq5qk 177 4 antibody antibody NN cord-298078-uqrwq5qk 177 5 ( ( -LRB- cord-298078-uqrwq5qk 177 6 BD BD NNP cord-298078-uqrwq5qk 177 7 Biosciences Biosciences NNP cord-298078-uqrwq5qk 177 8 ) ) -RRB- cord-298078-uqrwq5qk 177 9 was be VBD cord-298078-uqrwq5qk 177 10 used use VBN cord-298078-uqrwq5qk 177 11 for for IN cord-298078-uqrwq5qk 177 12 Western western JJ cord-298078-uqrwq5qk 177 13 blot blot NN cord-298078-uqrwq5qk 177 14 analysis analysis NN cord-298078-uqrwq5qk 177 15 . . . cord-298078-uqrwq5qk 178 1 Proteins protein NNS cord-298078-uqrwq5qk 178 2 in in IN cord-298078-uqrwq5qk 178 3 sample sample NN cord-298078-uqrwq5qk 178 4 buffer buffer NN cord-298078-uqrwq5qk 178 5 were be VBD cord-298078-uqrwq5qk 178 6 resolved resolve VBN cord-298078-uqrwq5qk 178 7 by by IN cord-298078-uqrwq5qk 178 8 10 10 CD cord-298078-uqrwq5qk 178 9 % % NN cord-298078-uqrwq5qk 178 10 SDS SDS NNP cord-298078-uqrwq5qk 178 11 - - HYPH cord-298078-uqrwq5qk 178 12 PAGE PAGE NNP cord-298078-uqrwq5qk 178 13 , , , cord-298078-uqrwq5qk 178 14 transferred transfer VBN cord-298078-uqrwq5qk 178 15 to to IN cord-298078-uqrwq5qk 178 16 a a DT cord-298078-uqrwq5qk 178 17 polyvinylidene polyvinylidene NN cord-298078-uqrwq5qk 178 18 difluoride difluoride NN cord-298078-uqrwq5qk 178 19 membrane membrane NN cord-298078-uqrwq5qk 178 20 , , , cord-298078-uqrwq5qk 178 21 immunostained immunostaine VBN cord-298078-uqrwq5qk 178 22 by by IN cord-298078-uqrwq5qk 178 23 specific specific JJ cord-298078-uqrwq5qk 178 24 antibodies antibody NNS cord-298078-uqrwq5qk 178 25 and and CC cord-298078-uqrwq5qk 178 26 visualized visualize VBD cord-298078-uqrwq5qk 178 27 using use VBG cord-298078-uqrwq5qk 178 28 a a DT cord-298078-uqrwq5qk 178 29 chemiluminescent chemiluminescent NN cord-298078-uqrwq5qk 178 30 substrate substrate NN cord-298078-uqrwq5qk 178 31 . . . cord-298078-uqrwq5qk 179 1 GST gst NN cord-298078-uqrwq5qk 179 2 - - HYPH cord-298078-uqrwq5qk 179 3 pull pull VB cord-298078-uqrwq5qk 179 4 down down RP cord-298078-uqrwq5qk 179 5 assay assay NN cord-298078-uqrwq5qk 179 6 was be VBD cord-298078-uqrwq5qk 179 7 performed perform VBN cord-298078-uqrwq5qk 179 8 for for IN cord-298078-uqrwq5qk 179 9 confirmation confirmation NN cord-298078-uqrwq5qk 179 10 of of IN cord-298078-uqrwq5qk 179 11 direct direct JJ cord-298078-uqrwq5qk 179 12 RNA RNA NNP cord-298078-uqrwq5qk 179 13 - - HYPH cord-298078-uqrwq5qk 179 14 protein protein NN cord-298078-uqrwq5qk 179 15 interaction interaction NN cord-298078-uqrwq5qk 179 16 . . . cord-298078-uqrwq5qk 180 1 Radiolabeled Radiolabeled NNP cord-298078-uqrwq5qk 180 2 RNA RNA NNP cord-298078-uqrwq5qk 180 3 transcript transcript NN cord-298078-uqrwq5qk 180 4 ( ( -LRB- cord-298078-uqrwq5qk 180 5 5610 5610 CD cord-298078-uqrwq5qk 180 6 4 4 CD cord-298078-uqrwq5qk 180 7 c.p.m c.p.m NN cord-298078-uqrwq5qk 180 8 ) ) -RRB- cord-298078-uqrwq5qk 180 9 and and CC cord-298078-uqrwq5qk 180 10 purified purify VBN cord-298078-uqrwq5qk 180 11 proteins protein NNS cord-298078-uqrwq5qk 180 12 were be VBD cord-298078-uqrwq5qk 180 13 incubated incubate VBN cord-298078-uqrwq5qk 180 14 at at IN cord-298078-uqrwq5qk 180 15 room room NN cord-298078-uqrwq5qk 180 16 immune immune NN cord-298078-uqrwq5qk 180 17 complex complex NN cord-298078-uqrwq5qk 180 18 was be VBD cord-298078-uqrwq5qk 180 19 normalized normalize VBN cord-298078-uqrwq5qk 180 20 with with IN cord-298078-uqrwq5qk 180 21 input input NN cord-298078-uqrwq5qk 180 22 PK PK NNP cord-298078-uqrwq5qk 180 23 RNA RNA NNP cord-298078-uqrwq5qk 180 24 level level NN cord-298078-uqrwq5qk 180 25 and and CC cord-298078-uqrwq5qk 180 26 presented present VBN cord-298078-uqrwq5qk 180 27 as as IN cord-298078-uqrwq5qk 180 28 relative relative JJ cord-298078-uqrwq5qk 180 29 enrichment enrichment NN cord-298078-uqrwq5qk 180 30 in in IN cord-298078-uqrwq5qk 180 31 comparison comparison NN cord-298078-uqrwq5qk 180 32 to to IN cord-298078-uqrwq5qk 180 33 mutant mutant JJ cord-298078-uqrwq5qk 180 34 PK PK NNP cord-298078-uqrwq5qk 180 35 RNA RNA NNP cord-298078-uqrwq5qk 180 36 ( ( -LRB- cord-298078-uqrwq5qk 180 37 MT MT NNP cord-298078-uqrwq5qk 180 38 ) ) -RRB- cord-298078-uqrwq5qk 180 39 . . . cord-298078-uqrwq5qk 181 1 ( ( -LRB- cord-298078-uqrwq5qk 181 2 D D NNP cord-298078-uqrwq5qk 181 3 ) ) -RRB- cord-298078-uqrwq5qk 181 4 RNA rna NN cord-298078-uqrwq5qk 181 5 immunoprecipitation immunoprecipitation NN cord-298078-uqrwq5qk 181 6 assay assay NN cord-298078-uqrwq5qk 181 7 in in IN cord-298078-uqrwq5qk 181 8 HEK293 HEK293 NNP cord-298078-uqrwq5qk 181 9 T T NNP cord-298078-uqrwq5qk 181 10 cells cell NNS cord-298078-uqrwq5qk 181 11 . . . cord-298078-uqrwq5qk 182 1 Reporter reporter NN cord-298078-uqrwq5qk 182 2 plasmids plasmid NNS cord-298078-uqrwq5qk 182 3 ( ( -LRB- cord-298078-uqrwq5qk 182 4 wild wild JJ cord-298078-uqrwq5qk 182 5 - - HYPH cord-298078-uqrwq5qk 182 6 type type NN cord-298078-uqrwq5qk 182 7 or or CC cord-298078-uqrwq5qk 182 8 mutant mutant JJ cord-298078-uqrwq5qk 182 9 IBV IBV NNP cord-298078-uqrwq5qk 182 10 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 182 11 plasmid plasmid NN cord-298078-uqrwq5qk 182 12 ) ) -RRB- cord-298078-uqrwq5qk 182 13 were be VBD cord-298078-uqrwq5qk 182 14 transfected transfecte VBN cord-298078-uqrwq5qk 182 15 into into IN cord-298078-uqrwq5qk 182 16 HEK293 HEK293 NNP cord-298078-uqrwq5qk 182 17 T T NNP cord-298078-uqrwq5qk 182 18 cells cell NNS cord-298078-uqrwq5qk 182 19 and and CC cord-298078-uqrwq5qk 182 20 fixed fix VBN cord-298078-uqrwq5qk 182 21 with with IN cord-298078-uqrwq5qk 182 22 formaldehyde formaldehyde NN cord-298078-uqrwq5qk 182 23 . . . cord-298078-uqrwq5qk 183 1 Sonicated Sonicated NNP cord-298078-uqrwq5qk 183 2 lysates lysate NNS cord-298078-uqrwq5qk 183 3 were be VBD cord-298078-uqrwq5qk 183 4 then then RB cord-298078-uqrwq5qk 183 5 incubated incubate VBN cord-298078-uqrwq5qk 183 6 with with IN cord-298078-uqrwq5qk 183 7 the the DT cord-298078-uqrwq5qk 183 8 antibodies antibody NNS cord-298078-uqrwq5qk 183 9 as as IN cord-298078-uqrwq5qk 183 10 indicated indicate VBN cord-298078-uqrwq5qk 183 11 ( ( -LRB- cord-298078-uqrwq5qk 183 12 anti anti JJ cord-298078-uqrwq5qk 183 13 - - JJ cord-298078-uqrwq5qk 183 14 IgG igg JJ cord-298078-uqrwq5qk 183 15 or or CC cord-298078-uqrwq5qk 183 16 anti anti JJ cord-298078-uqrwq5qk 183 17 - - JJ cord-298078-uqrwq5qk 183 18 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 183 19 ) ) -RRB- cord-298078-uqrwq5qk 183 20 . . . cord-298078-uqrwq5qk 184 1 RNA rna NN cord-298078-uqrwq5qk 184 2 - - HYPH cord-298078-uqrwq5qk 184 3 protein protein NN cord-298078-uqrwq5qk 184 4 complexes complex NNS cord-298078-uqrwq5qk 184 5 were be VBD cord-298078-uqrwq5qk 184 6 precipitated precipitate VBN cord-298078-uqrwq5qk 184 7 with with IN cord-298078-uqrwq5qk 184 8 protein protein NN cord-298078-uqrwq5qk 184 9 - - HYPH cord-298078-uqrwq5qk 184 10 G g NN cord-298078-uqrwq5qk 184 11 beads bead NNS cord-298078-uqrwq5qk 184 12 . . . cord-298078-uqrwq5qk 185 1 Bound Bound NNP cord-298078-uqrwq5qk 185 2 RNA RNA NNP cord-298078-uqrwq5qk 185 3 was be VBD cord-298078-uqrwq5qk 185 4 extracted extract VBN cord-298078-uqrwq5qk 185 5 from from IN cord-298078-uqrwq5qk 185 6 the the DT cord-298078-uqrwq5qk 185 7 immune immune JJ cord-298078-uqrwq5qk 185 8 complexes complex NNS cord-298078-uqrwq5qk 185 9 and and CC cord-298078-uqrwq5qk 185 10 analyzed analyze VBN cord-298078-uqrwq5qk 185 11 by by IN cord-298078-uqrwq5qk 185 12 RT RT NNP cord-298078-uqrwq5qk 185 13 - - HYPH cord-298078-uqrwq5qk 185 14 PCR PCR NNP cord-298078-uqrwq5qk 185 15 and and CC cord-298078-uqrwq5qk 185 16 qRT qRT NNP cord-298078-uqrwq5qk 185 17 - - HYPH cord-298078-uqrwq5qk 185 18 PCR PCR NNP cord-298078-uqrwq5qk 185 19 . . . cord-298078-uqrwq5qk 186 1 Immunoprecipitated Immunoprecipitated NNP cord-298078-uqrwq5qk 186 2 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 186 3 protein protein NN cord-298078-uqrwq5qk 186 4 was be VBD cord-298078-uqrwq5qk 186 5 shown show VBN cord-298078-uqrwq5qk 186 6 by by IN cord-298078-uqrwq5qk 186 7 immunoblotting immunoblotte VBG cord-298078-uqrwq5qk 186 8 ( ( -LRB- cord-298078-uqrwq5qk 186 9 IB IB NNP cord-298078-uqrwq5qk 186 10 ) ) -RRB- cord-298078-uqrwq5qk 186 11 . . . cord-298078-uqrwq5qk 187 1 Relative relative JJ cord-298078-uqrwq5qk 187 2 enrichment enrichment NN cord-298078-uqrwq5qk 187 3 of of IN cord-298078-uqrwq5qk 187 4 binding bind VBG cord-298078-uqrwq5qk 187 5 was be VBD cord-298078-uqrwq5qk 187 6 shown show VBN cord-298078-uqrwq5qk 187 7 as as IN cord-298078-uqrwq5qk 187 8 in in IN cord-298078-uqrwq5qk 187 9 ( ( -LRB- cord-298078-uqrwq5qk 187 10 C C NNP cord-298078-uqrwq5qk 187 11 ) ) -RRB- cord-298078-uqrwq5qk 187 12 . . . cord-298078-uqrwq5qk 188 1 doi:10.1371 doi:10.1371 NNP cord-298078-uqrwq5qk 189 1 /journal.pone.0024067.g003 /journal.pone.0024067.g003 : cord-298078-uqrwq5qk 189 2 temperature temperature NN cord-298078-uqrwq5qk 189 3 for for IN cord-298078-uqrwq5qk 189 4 1 1 CD cord-298078-uqrwq5qk 189 5 h h NN cord-298078-uqrwq5qk 189 6 in in IN cord-298078-uqrwq5qk 189 7 a a DT cord-298078-uqrwq5qk 189 8 binding binding JJ cord-298078-uqrwq5qk 189 9 buffer buffer NN cord-298078-uqrwq5qk 189 10 containing contain VBG cord-298078-uqrwq5qk 189 11 25 25 CD cord-298078-uqrwq5qk 189 12 mM mm CD cord-298078-uqrwq5qk 189 13 HEPES HEPES NNP cord-298078-uqrwq5qk 189 14 pH pH NNP cord-298078-uqrwq5qk 189 15 7.5 7.5 CD cord-298078-uqrwq5qk 189 16 , , , cord-298078-uqrwq5qk 189 17 100 100 CD cord-298078-uqrwq5qk 189 18 mM mm CD cord-298078-uqrwq5qk 189 19 NaCl nacl NN cord-298078-uqrwq5qk 189 20 and and CC cord-298078-uqrwq5qk 189 21 1 1 CD cord-298078-uqrwq5qk 189 22 mM mm CD cord-298078-uqrwq5qk 189 23 MgCl MgCl NNP cord-298078-uqrwq5qk 189 24 2 2 CD cord-298078-uqrwq5qk 189 25 . . . cord-298078-uqrwq5qk 190 1 RNA rna NN cord-298078-uqrwq5qk 190 2 - - HYPH cord-298078-uqrwq5qk 190 3 protein protein NN cord-298078-uqrwq5qk 190 4 complexes complex NNS cord-298078-uqrwq5qk 190 5 were be VBD cord-298078-uqrwq5qk 190 6 pulled pull VBN cord-298078-uqrwq5qk 190 7 down down RP cord-298078-uqrwq5qk 190 8 by by IN cord-298078-uqrwq5qk 190 9 Glutathione Glutathione NNP cord-298078-uqrwq5qk 190 10 Sepharose Sepharose NNP cord-298078-uqrwq5qk 190 11 4B 4b NN cord-298078-uqrwq5qk 190 12 ( ( -LRB- cord-298078-uqrwq5qk 190 13 GE GE NNP cord-298078-uqrwq5qk 190 14 Healthcare Healthcare NNP cord-298078-uqrwq5qk 190 15 ) ) -RRB- cord-298078-uqrwq5qk 191 1 pre pre VB cord-298078-uqrwq5qk 191 2 - - VBN cord-298078-uqrwq5qk 191 3 blocked block VBN cord-298078-uqrwq5qk 191 4 with with IN cord-298078-uqrwq5qk 191 5 tRNA trna NN cord-298078-uqrwq5qk 191 6 for for IN cord-298078-uqrwq5qk 191 7 30 30 CD cord-298078-uqrwq5qk 191 8 min min NN cord-298078-uqrwq5qk 191 9 . . . cord-298078-uqrwq5qk 192 1 Following follow VBG cord-298078-uqrwq5qk 192 2 washing washing NN cord-298078-uqrwq5qk 192 3 using use VBG cord-298078-uqrwq5qk 192 4 binding bind VBG cord-298078-uqrwq5qk 192 5 buffer buffer NN cord-298078-uqrwq5qk 192 6 , , , cord-298078-uqrwq5qk 192 7 the the DT cord-298078-uqrwq5qk 192 8 radioactivity radioactivity NN cord-298078-uqrwq5qk 192 9 in in IN cord-298078-uqrwq5qk 192 10 the the DT cord-298078-uqrwq5qk 192 11 recovered recover VBN cord-298078-uqrwq5qk 192 12 pellets pellet NNS cord-298078-uqrwq5qk 192 13 was be VBD cord-298078-uqrwq5qk 192 14 measured measure VBN cord-298078-uqrwq5qk 192 15 by by IN cord-298078-uqrwq5qk 192 16 scintillation scintillation NN cord-298078-uqrwq5qk 192 17 counting counting NN cord-298078-uqrwq5qk 192 18 . . . cord-298078-uqrwq5qk 193 1 Construction construction NN cord-298078-uqrwq5qk 193 2 of of IN cord-298078-uqrwq5qk 193 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 193 4 expression expression NN cord-298078-uqrwq5qk 193 5 clone clone NN cord-298078-uqrwq5qk 193 6 and and CC cord-298078-uqrwq5qk 193 7 RNA RNA NNP cord-298078-uqrwq5qk 193 8 interference interference NN cord-298078-uqrwq5qk 193 9 cDNA cdna NN cord-298078-uqrwq5qk 193 10 for for IN cord-298078-uqrwq5qk 193 11 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 194 1 was be VBD cord-298078-uqrwq5qk 194 2 polymerase polymerase NN cord-298078-uqrwq5qk 194 3 chain chain NN cord-298078-uqrwq5qk 194 4 reaction reaction NN cord-298078-uqrwq5qk 194 5 ( ( -LRB- cord-298078-uqrwq5qk 194 6 PCR)amplified pcr)amplifie VBN cord-298078-uqrwq5qk 194 7 and and CC cord-298078-uqrwq5qk 194 8 ligated ligate VBN cord-298078-uqrwq5qk 194 9 into into IN cord-298078-uqrwq5qk 194 10 pCMV pcmv NN cord-298078-uqrwq5qk 194 11 - - HYPH cord-298078-uqrwq5qk 194 12 Tag2B tag2b JJ cord-298078-uqrwq5qk 194 13 vector vector NN cord-298078-uqrwq5qk 194 14 ( ( -LRB- cord-298078-uqrwq5qk 194 15 stratagene stratagene NNP cord-298078-uqrwq5qk 194 16 ) ) -RRB- cord-298078-uqrwq5qk 194 17 . . . cord-298078-uqrwq5qk 195 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 195 2 was be VBD cord-298078-uqrwq5qk 195 3 amplified amplify VBN cord-298078-uqrwq5qk 195 4 with with IN cord-298078-uqrwq5qk 195 5 primers primer NNS cord-298078-uqrwq5qk 195 6 59 59 CD cord-298078-uqrwq5qk 195 7 -CAGGATCCATGTC -CAGGATCCATGTC . cord-298078-uqrwq5qk 196 1 -TACCGTTCA-39 -TACCGTTCA-39 NNP cord-298078-uqrwq5qk 196 2 and and CC cord-298078-uqrwq5qk 196 3 59 59 CD cord-298078-uqrwq5qk 196 4 -CCGAATTCTCAGTCATCCCCA -CCGAATTCTCAGTCATCCCCA . cord-298078-uqrwq5qk 196 5 - - : cord-298078-uqrwq5qk 196 6 C-39 C-39 NNP cord-298078-uqrwq5qk 196 7 . . . cord-298078-uqrwq5qk 197 1 To to TO cord-298078-uqrwq5qk 197 2 knock knock VB cord-298078-uqrwq5qk 197 3 - - HYPH cord-298078-uqrwq5qk 197 4 down down RP cord-298078-uqrwq5qk 198 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 198 2 mRNA mrna NN cord-298078-uqrwq5qk 198 3 , , , cord-298078-uqrwq5qk 198 4 small small JJ cord-298078-uqrwq5qk 198 5 hairpin hairpin NN cord-298078-uqrwq5qk 198 6 RNA RNA NNP cord-298078-uqrwq5qk 198 7 ( ( -LRB- cord-298078-uqrwq5qk 198 8 shRNA shrna NN cord-298078-uqrwq5qk 198 9 ) ) -RRB- cord-298078-uqrwq5qk 198 10 was be VBD cord-298078-uqrwq5qk 198 11 designed design VBN cord-298078-uqrwq5qk 198 12 using use VBG cord-298078-uqrwq5qk 198 13 the the DT cord-298078-uqrwq5qk 198 14 Ambion Ambion NNP cord-298078-uqrwq5qk 198 15 small small JJ cord-298078-uqrwq5qk 198 16 interfering interfere VBG cord-298078-uqrwq5qk 198 17 RNA RNA NNP cord-298078-uqrwq5qk 198 18 ( ( -LRB- cord-298078-uqrwq5qk 198 19 siRNA siRNA NNP cord-298078-uqrwq5qk 198 20 ) ) -RRB- cord-298078-uqrwq5qk 198 21 converter converter NNP cord-298078-uqrwq5qk 198 22 website website NN cord-298078-uqrwq5qk 198 23 . . . cord-298078-uqrwq5qk 199 1 The the DT cord-298078-uqrwq5qk 199 2 ANXA2 anxa2 FW cord-298078-uqrwq5qk 199 3 target target NN cord-298078-uqrwq5qk 199 4 sequence sequence NN cord-298078-uqrwq5qk 199 5 was be VBD cord-298078-uqrwq5qk 199 6 59-UGCAUAUGGGUCUGUCAA-39 59-UGCAUAUGGGUCUGUCAA-39 NNP cord-298078-uqrwq5qk 199 7 corresponded correspond VBD cord-298078-uqrwq5qk 199 8 to to IN cord-298078-uqrwq5qk 199 9 coding code VBG cord-298078-uqrwq5qk 199 10 region region NN cord-298078-uqrwq5qk 199 11 nucleotides nucleotide NNS cord-298078-uqrwq5qk 199 12 66 66 CD cord-298078-uqrwq5qk 199 13 - - SYM cord-298078-uqrwq5qk 199 14 83 83 CD cord-298078-uqrwq5qk 199 15 . . . cord-298078-uqrwq5qk 200 1 To to TO cord-298078-uqrwq5qk 200 2 make make VB cord-298078-uqrwq5qk 200 3 pSUPER psuper NN cord-298078-uqrwq5qk 200 4 - - HYPH cord-298078-uqrwq5qk 200 5 ANXA2 anxa2 NN cord-298078-uqrwq5qk 200 6 ( ( -LRB- cord-298078-uqrwq5qk 200 7 shANX2 shanx2 NN cord-298078-uqrwq5qk 200 8 - - HYPH cord-298078-uqrwq5qk 200 9 1 1 CD cord-298078-uqrwq5qk 200 10 ) ) -RRB- cord-298078-uqrwq5qk 200 11 , , , cord-298078-uqrwq5qk 200 12 specific specific JJ cord-298078-uqrwq5qk 200 13 oligonucleotides oligonucleotide NNS cord-298078-uqrwq5qk 200 14 were be VBD cord-298078-uqrwq5qk 200 15 synthesized synthesize VBN cord-298078-uqrwq5qk 200 16 ( ( -LRB- cord-298078-uqrwq5qk 200 17 Bioneer Bioneer NNP cord-298078-uqrwq5qk 200 18 , , , cord-298078-uqrwq5qk 200 19 Korea Korea NNP cord-298078-uqrwq5qk 200 20 ) ) -RRB- cord-298078-uqrwq5qk 200 21 and and CC cord-298078-uqrwq5qk 200 22 ligated ligate VBD cord-298078-uqrwq5qk 200 23 to to IN cord-298078-uqrwq5qk 200 24 pSUPER psuper NN cord-298078-uqrwq5qk 200 25 vector vector NN cord-298078-uqrwq5qk 200 26 as as IN cord-298078-uqrwq5qk 200 27 previously previously RB cord-298078-uqrwq5qk 200 28 reported report VBN cord-298078-uqrwq5qk 200 29 [ [ -LRB- cord-298078-uqrwq5qk 200 30 45 45 CD cord-298078-uqrwq5qk 200 31 ] ] -RRB- cord-298078-uqrwq5qk 200 32 . . . cord-298078-uqrwq5qk 201 1 Also also RB cord-298078-uqrwq5qk 201 2 , , , cord-298078-uqrwq5qk 201 3 two two CD cord-298078-uqrwq5qk 201 4 different different JJ cord-298078-uqrwq5qk 201 5 siRNAs sirna NNS cord-298078-uqrwq5qk 201 6 corresponding correspond VBG cord-298078-uqrwq5qk 201 7 to to IN cord-298078-uqrwq5qk 201 8 coding code VBG cord-298078-uqrwq5qk 201 9 region region NN cord-298078-uqrwq5qk 201 10 nucleotides nucleotide NNS cord-298078-uqrwq5qk 201 11 109 109 CD cord-298078-uqrwq5qk 201 12 - - SYM cord-298078-uqrwq5qk 201 13 129 129 CD cord-298078-uqrwq5qk 201 14 ( ( -LRB- cord-298078-uqrwq5qk 201 15 siANX2 sianx2 NN cord-298078-uqrwq5qk 201 16 - - HYPH cord-298078-uqrwq5qk 201 17 2 2 CD cord-298078-uqrwq5qk 201 18 : : : cord-298078-uqrwq5qk 201 19 59-CGGGAUGCUUUGAACAUUGAA-39 59-CGGGAUGCUUUGAACAUUGAA-39 NNP cord-298078-uqrwq5qk 201 20 ) ) -RRB- cord-298078-uqrwq5qk 201 21 and and CC cord-298078-uqrwq5qk 201 22 772 772 CD cord-298078-uqrwq5qk 201 23 - - HYPH cord-298078-uqrwq5qk 201 24 792 792 CD cord-298078-uqrwq5qk 201 25 ( ( -LRB- cord-298078-uqrwq5qk 201 26 siANX2 sianx2 NN cord-298078-uqrwq5qk 201 27 - - HYPH cord-298078-uqrwq5qk 201 28 3 3 CD cord-298078-uqrwq5qk 201 29 : : : cord-298078-uqrwq5qk 201 30 59-AACCUGGUUCAGUGCAUUCAG-39 59-AACCUGGUUCAGUGCAUUCAG-39 NNP cord-298078-uqrwq5qk 201 31 ) ) -RRB- cord-298078-uqrwq5qk 201 32 were be VBD cord-298078-uqrwq5qk 201 33 designed design VBN cord-298078-uqrwq5qk 201 34 . . . cord-298078-uqrwq5qk 202 1 siRNA siRNA NNP cord-298078-uqrwq5qk 202 2 duplex duplex NNP cord-298078-uqrwq5qk 202 3 were be VBD cord-298078-uqrwq5qk 202 4 chemically chemically RB cord-298078-uqrwq5qk 202 5 synthesized synthesize VBN cord-298078-uqrwq5qk 202 6 and and CC cord-298078-uqrwq5qk 202 7 contain contain VBP cord-298078-uqrwq5qk 202 8 dTdT dtdt CD cord-298078-uqrwq5qk 202 9 39 39 CD cord-298078-uqrwq5qk 202 10 overhangs overhang NNS cord-298078-uqrwq5qk 202 11 ( ( -LRB- cord-298078-uqrwq5qk 202 12 Bioneer Bioneer NNP cord-298078-uqrwq5qk 202 13 , , , cord-298078-uqrwq5qk 202 14 Korea Korea NNP cord-298078-uqrwq5qk 202 15 ) ) -RRB- cord-298078-uqrwq5qk 202 16 . . . cord-298078-uqrwq5qk 203 1 Purification purification NN cord-298078-uqrwq5qk 203 2 of of IN cord-298078-uqrwq5qk 203 3 recombinant recombinant JJ cord-298078-uqrwq5qk 203 4 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 203 5 protein protein NN cord-298078-uqrwq5qk 203 6 cDNA cdna NN cord-298078-uqrwq5qk 203 7 of of IN cord-298078-uqrwq5qk 203 8 the the DT cord-298078-uqrwq5qk 203 9 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 203 10 protein protein NN cord-298078-uqrwq5qk 203 11 was be VBD cord-298078-uqrwq5qk 203 12 PCR PCR NNP cord-298078-uqrwq5qk 203 13 amplified amplify VBN cord-298078-uqrwq5qk 203 14 and and CC cord-298078-uqrwq5qk 203 15 inserted insert VBN cord-298078-uqrwq5qk 203 16 into into IN cord-298078-uqrwq5qk 203 17 the the DT cord-298078-uqrwq5qk 203 18 pGEX-4T-1 pGEX-4T-1 NNP cord-298078-uqrwq5qk 203 19 vector vector NN cord-298078-uqrwq5qk 203 20 ( ( -LRB- cord-298078-uqrwq5qk 203 21 GE GE NNP cord-298078-uqrwq5qk 203 22 Healthcare Healthcare NNP cord-298078-uqrwq5qk 203 23 ) ) -RRB- cord-298078-uqrwq5qk 203 24 , , , cord-298078-uqrwq5qk 203 25 followed follow VBN cord-298078-uqrwq5qk 203 26 by by IN cord-298078-uqrwq5qk 203 27 the the DT cord-298078-uqrwq5qk 203 28 transformation transformation NN cord-298078-uqrwq5qk 203 29 of of IN cord-298078-uqrwq5qk 203 30 the the DT cord-298078-uqrwq5qk 203 31 vectors vector NNS cord-298078-uqrwq5qk 203 32 into into IN cord-298078-uqrwq5qk 203 33 the the DT cord-298078-uqrwq5qk 203 34 protease protease NN cord-298078-uqrwq5qk 203 35 deficient deficient JJ cord-298078-uqrwq5qk 203 36 strain strain NN cord-298078-uqrwq5qk 204 1 BL21 BL21 NNP cord-298078-uqrwq5qk 204 2 . . . cord-298078-uqrwq5qk 205 1 GST GST NNP cord-298078-uqrwq5qk 205 2 - - HYPH cord-298078-uqrwq5qk 205 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 205 4 and and CC cord-298078-uqrwq5qk 205 5 GST GST NNP cord-298078-uqrwq5qk 205 6 protein protein NN cord-298078-uqrwq5qk 205 7 was be VBD cord-298078-uqrwq5qk 205 8 purified purify VBN cord-298078-uqrwq5qk 205 9 according accord VBG cord-298078-uqrwq5qk 205 10 to to IN cord-298078-uqrwq5qk 205 11 manufacturer manufacturer NN cord-298078-uqrwq5qk 205 12 's 's POS cord-298078-uqrwq5qk 205 13 instructions instruction NNS cord-298078-uqrwq5qk 205 14 . . . cord-298078-uqrwq5qk 206 1 The the DT cord-298078-uqrwq5qk 206 2 basic basic JJ cord-298078-uqrwq5qk 206 3 procedure procedure NN cord-298078-uqrwq5qk 206 4 for for IN cord-298078-uqrwq5qk 206 5 EMSA EMSA NNP cord-298078-uqrwq5qk 206 6 was be VBD cord-298078-uqrwq5qk 206 7 previously previously RB cord-298078-uqrwq5qk 206 8 described describe VBN cord-298078-uqrwq5qk 206 9 [ [ -LRB- cord-298078-uqrwq5qk 206 10 46 46 CD cord-298078-uqrwq5qk 206 11 ] ] -RRB- cord-298078-uqrwq5qk 206 12 . . . cord-298078-uqrwq5qk 207 1 Briefly briefly RB cord-298078-uqrwq5qk 207 2 , , , cord-298078-uqrwq5qk 207 3 RNA RNA NNP cord-298078-uqrwq5qk 207 4 was be VBD cord-298078-uqrwq5qk 207 5 in in IN cord-298078-uqrwq5qk 207 6 vitro vitro FW cord-298078-uqrwq5qk 207 7 transcribed transcribe VBN cord-298078-uqrwq5qk 207 8 and and CC cord-298078-uqrwq5qk 207 9 labeled label VBN cord-298078-uqrwq5qk 207 10 in in IN cord-298078-uqrwq5qk 207 11 vitro vitro FW cord-298078-uqrwq5qk 207 12 with with IN cord-298078-uqrwq5qk 207 13 T7 T7 NNP cord-298078-uqrwq5qk 207 14 RNA RNA NNP cord-298078-uqrwq5qk 207 15 polymerase polymerase NN cord-298078-uqrwq5qk 207 16 ( ( -LRB- cord-298078-uqrwq5qk 207 17 Ambion Ambion NNP cord-298078-uqrwq5qk 207 18 ) ) -RRB- cord-298078-uqrwq5qk 207 19 with with IN cord-298078-uqrwq5qk 207 20 [ [ -LRB- cord-298078-uqrwq5qk 207 21 a-32 a-32 NNP cord-298078-uqrwq5qk 207 22 P]ATP P]ATP NNP cord-298078-uqrwq5qk 207 23 . . . cord-298078-uqrwq5qk 208 1 32 32 CD cord-298078-uqrwq5qk 208 2 P p NN cord-298078-uqrwq5qk 208 3 labeled label VBD cord-298078-uqrwq5qk 208 4 IBV IBV NNP cord-298078-uqrwq5qk 208 5 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 208 6 RNA RNA NNP cord-298078-uqrwq5qk 208 7 ( ( -LRB- cord-298078-uqrwq5qk 208 8 1.1610 1.1610 CD cord-298078-uqrwq5qk 208 9 5 5 CD cord-298078-uqrwq5qk 208 10 c.p.m c.p.m NNS cord-298078-uqrwq5qk 208 11 ) ) -RRB- cord-298078-uqrwq5qk 208 12 and and CC cord-298078-uqrwq5qk 208 13 purified purify VBN cord-298078-uqrwq5qk 208 14 GST GST NNP cord-298078-uqrwq5qk 208 15 - - HYPH cord-298078-uqrwq5qk 208 16 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 208 17 or or CC cord-298078-uqrwq5qk 208 18 GST GST NNP cord-298078-uqrwq5qk 208 19 protein protein NN cord-298078-uqrwq5qk 208 20 were be VBD cord-298078-uqrwq5qk 208 21 incubated incubate VBN cord-298078-uqrwq5qk 208 22 at at IN cord-298078-uqrwq5qk 208 23 room room NN cord-298078-uqrwq5qk 208 24 temperature temperature NN cord-298078-uqrwq5qk 208 25 for for IN cord-298078-uqrwq5qk 208 26 30 30 CD cord-298078-uqrwq5qk 208 27 min min NN cord-298078-uqrwq5qk 208 28 in in IN cord-298078-uqrwq5qk 208 29 a a DT cord-298078-uqrwq5qk 208 30 binding binding JJ cord-298078-uqrwq5qk 208 31 buffer buffer NN cord-298078-uqrwq5qk 208 32 containing contain VBG cord-298078-uqrwq5qk 208 33 25 25 CD cord-298078-uqrwq5qk 208 34 mM mm CD cord-298078-uqrwq5qk 208 35 HEPES HEPES NNP cord-298078-uqrwq5qk 208 36 pH pH NNP cord-298078-uqrwq5qk 208 37 7.5 7.5 CD cord-298078-uqrwq5qk 208 38 , , , cord-298078-uqrwq5qk 208 39 100 100 CD cord-298078-uqrwq5qk 208 40 mM mm CD cord-298078-uqrwq5qk 208 41 NaCl nacl NN cord-298078-uqrwq5qk 208 42 , , , cord-298078-uqrwq5qk 208 43 1 1 CD cord-298078-uqrwq5qk 208 44 mM mm CD cord-298078-uqrwq5qk 208 45 MgCl MgCl NNP cord-298078-uqrwq5qk 208 46 2 2 CD cord-298078-uqrwq5qk 208 47 and and CC cord-298078-uqrwq5qk 208 48 4 4 CD cord-298078-uqrwq5qk 208 49 % % NN cord-298078-uqrwq5qk 208 50 glycerol glycerol NN cord-298078-uqrwq5qk 208 51 . . . cord-298078-uqrwq5qk 209 1 RNA rna NN cord-298078-uqrwq5qk 209 2 - - HYPH cord-298078-uqrwq5qk 209 3 protein protein NN cord-298078-uqrwq5qk 209 4 complexes complex NNS cord-298078-uqrwq5qk 209 5 were be VBD cord-298078-uqrwq5qk 209 6 resolved resolve VBN cord-298078-uqrwq5qk 209 7 on on IN cord-298078-uqrwq5qk 209 8 5 5 CD cord-298078-uqrwq5qk 209 9 % % NN cord-298078-uqrwq5qk 209 10 native native JJ cord-298078-uqrwq5qk 209 11 polyacrylamide polyacrylamide NN cord-298078-uqrwq5qk 209 12 gels gel NNS cord-298078-uqrwq5qk 209 13 . . . cord-298078-uqrwq5qk 210 1 Gels gel NNS cord-298078-uqrwq5qk 210 2 were be VBD cord-298078-uqrwq5qk 210 3 dried dry VBN cord-298078-uqrwq5qk 210 4 and and CC cord-298078-uqrwq5qk 210 5 analyzed analyze VBN cord-298078-uqrwq5qk 210 6 by by IN cord-298078-uqrwq5qk 210 7 autoradiography autoradiography NNP cord-298078-uqrwq5qk 210 8 . . . cord-298078-uqrwq5qk 211 1 The the DT cord-298078-uqrwq5qk 211 2 basic basic JJ cord-298078-uqrwq5qk 211 3 procedure procedure NN cord-298078-uqrwq5qk 211 4 for for IN cord-298078-uqrwq5qk 211 5 RNA RNA NNP cord-298078-uqrwq5qk 211 6 - - HYPH cord-298078-uqrwq5qk 211 7 IP IP NNP cord-298078-uqrwq5qk 211 8 was be VBD cord-298078-uqrwq5qk 211 9 previously previously RB cord-298078-uqrwq5qk 211 10 described describe VBN cord-298078-uqrwq5qk 211 11 [ [ -LRB- cord-298078-uqrwq5qk 211 12 46 46 CD cord-298078-uqrwq5qk 211 13 ] ] -RRB- cord-298078-uqrwq5qk 211 14 . . . cord-298078-uqrwq5qk 212 1 Briefly briefly RB cord-298078-uqrwq5qk 212 2 , , , cord-298078-uqrwq5qk 212 3 LNCaP lncap NN cord-298078-uqrwq5qk 212 4 and and CC cord-298078-uqrwq5qk 212 5 HEK293 HEK293 NNP cord-298078-uqrwq5qk 212 6 T t NN cord-298078-uqrwq5qk 212 7 cells cell NNS cord-298078-uqrwq5qk 212 8 were be VBD cord-298078-uqrwq5qk 212 9 transiently transiently RB cord-298078-uqrwq5qk 212 10 cotransfected cotransfecte VBN cord-298078-uqrwq5qk 212 11 with with IN cord-298078-uqrwq5qk 212 12 wild wild JJ cord-298078-uqrwq5qk 212 13 - - HYPH cord-298078-uqrwq5qk 212 14 type type NN cord-298078-uqrwq5qk 212 15 of of IN cord-298078-uqrwq5qk 212 16 mutant mutant JJ cord-298078-uqrwq5qk 212 17 IBV IBV NNP cord-298078-uqrwq5qk 212 18 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 212 19 reporters reporter NNS cord-298078-uqrwq5qk 212 20 and and CC cord-298078-uqrwq5qk 212 21 pCMV pcmv NN cord-298078-uqrwq5qk 212 22 Tag2B Tag2B NNP cord-298078-uqrwq5qk 212 23 vector vector NN cord-298078-uqrwq5qk 212 24 ( ( -LRB- cord-298078-uqrwq5qk 212 25 Flag Flag NNP cord-298078-uqrwq5qk 212 26 - - HYPH cord-298078-uqrwq5qk 212 27 Vector Vector NNP cord-298078-uqrwq5qk 212 28 ) ) -RRB- cord-298078-uqrwq5qk 212 29 or or CC cord-298078-uqrwq5qk 212 30 FLAG flag NN cord-298078-uqrwq5qk 212 31 - - HYPH cord-298078-uqrwq5qk 212 32 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 212 33 plasmids plasmid NNS cord-298078-uqrwq5qk 212 34 , , , cord-298078-uqrwq5qk 212 35 and and CC cord-298078-uqrwq5qk 212 36 incubated incubate VBN cord-298078-uqrwq5qk 212 37 with with IN cord-298078-uqrwq5qk 212 38 1 1 CD cord-298078-uqrwq5qk 212 39 % % NN cord-298078-uqrwq5qk 212 40 formaldehyde formaldehyde NN cord-298078-uqrwq5qk 212 41 for for IN cord-298078-uqrwq5qk 212 42 crosslinking crosslinke VBG cord-298078-uqrwq5qk 212 43 . . . cord-298078-uqrwq5qk 213 1 Sonicated Sonicated NNP cord-298078-uqrwq5qk 213 2 lysates lysate NNS cord-298078-uqrwq5qk 213 3 were be VBD cord-298078-uqrwq5qk 213 4 immunoprecipitated immunoprecipitate VBN cord-298078-uqrwq5qk 213 5 with with IN cord-298078-uqrwq5qk 213 6 FLAG FLAG NNP cord-298078-uqrwq5qk 213 7 M2 M2 NNP cord-298078-uqrwq5qk 213 8 agarose agarose JJ cord-298078-uqrwq5qk 213 9 beads bead NNS cord-298078-uqrwq5qk 213 10 ( ( -LRB- cord-298078-uqrwq5qk 213 11 Sigma Sigma NNP cord-298078-uqrwq5qk 213 12 Aldrich Aldrich NNP cord-298078-uqrwq5qk 213 13 ) ) -RRB- cord-298078-uqrwq5qk 213 14 or or CC cord-298078-uqrwq5qk 213 15 with with IN cord-298078-uqrwq5qk 213 16 antibodies antibody NNS cord-298078-uqrwq5qk 213 17 as as IN cord-298078-uqrwq5qk 213 18 indicated indicate VBN cord-298078-uqrwq5qk 213 19 ( ( -LRB- cord-298078-uqrwq5qk 213 20 anti anti JJ cord-298078-uqrwq5qk 213 21 - - JJ cord-298078-uqrwq5qk 213 22 normal normal JJ cord-298078-uqrwq5qk 213 23 mouse mouse NN cord-298078-uqrwq5qk 213 24 IgG igg NN cord-298078-uqrwq5qk 213 25 or or CC cord-298078-uqrwq5qk 213 26 anti anti JJ cord-298078-uqrwq5qk 213 27 - - JJ cord-298078-uqrwq5qk 213 28 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 213 29 ) ) -RRB- cord-298078-uqrwq5qk 213 30 . . . cord-298078-uqrwq5qk 214 1 Pellets pellet NNS cord-298078-uqrwq5qk 214 2 were be VBD cord-298078-uqrwq5qk 214 3 subsequently subsequently RB cord-298078-uqrwq5qk 214 4 incubated incubate VBN cord-298078-uqrwq5qk 214 5 at at IN cord-298078-uqrwq5qk 214 6 70uC 70uc CD cord-298078-uqrwq5qk 214 7 for for IN cord-298078-uqrwq5qk 214 8 1 1 CD cord-298078-uqrwq5qk 214 9 h h NN cord-298078-uqrwq5qk 214 10 to to TO cord-298078-uqrwq5qk 214 11 reverse reverse VB cord-298078-uqrwq5qk 214 12 the the DT cord-298078-uqrwq5qk 214 13 crosslinks crosslink NNS cord-298078-uqrwq5qk 214 14 , , , cord-298078-uqrwq5qk 214 15 and and CC cord-298078-uqrwq5qk 214 16 the the DT cord-298078-uqrwq5qk 214 17 RNA RNA NNP cord-298078-uqrwq5qk 214 18 was be VBD cord-298078-uqrwq5qk 214 19 purified purify VBN cord-298078-uqrwq5qk 214 20 with with IN cord-298078-uqrwq5qk 214 21 TRI TRI NNP cord-298078-uqrwq5qk 214 22 Reagent Reagent NNP cord-298078-uqrwq5qk 214 23 ( ( -LRB- cord-298078-uqrwq5qk 214 24 Ambion Ambion NNP cord-298078-uqrwq5qk 214 25 ) ) -RRB- cord-298078-uqrwq5qk 214 26 and and CC cord-298078-uqrwq5qk 214 27 treated treat VBN cord-298078-uqrwq5qk 214 28 with with IN cord-298078-uqrwq5qk 214 29 DNase DNase NNP cord-298078-uqrwq5qk 214 30 I i NN cord-298078-uqrwq5qk 214 31 ( ( -LRB- cord-298078-uqrwq5qk 214 32 Ambion ambion NN cord-298078-uqrwq5qk 214 33 ) ) -RRB- cord-298078-uqrwq5qk 214 34 to to TO cord-298078-uqrwq5qk 214 35 remove remove VB cord-298078-uqrwq5qk 214 36 reporter reporter NN cord-298078-uqrwq5qk 214 37 plasmid plasmid NN cord-298078-uqrwq5qk 214 38 DNA DNA NNP cord-298078-uqrwq5qk 214 39 . . . cord-298078-uqrwq5qk 215 1 After after IN cord-298078-uqrwq5qk 215 2 reverse reverse JJ cord-298078-uqrwq5qk 215 3 transcription transcription NN cord-298078-uqrwq5qk 215 4 , , , cord-298078-uqrwq5qk 215 5 cDNA cdna NN cord-298078-uqrwq5qk 215 6 was be VBD cord-298078-uqrwq5qk 215 7 amplified amplify VBN cord-298078-uqrwq5qk 215 8 by by IN cord-298078-uqrwq5qk 215 9 using use VBG cord-298078-uqrwq5qk 215 10 primer primer NN cord-298078-uqrwq5qk 215 11 pairs pair NNS cord-298078-uqrwq5qk 215 12 for for IN cord-298078-uqrwq5qk 215 13 IBV IBV NNP cord-298078-uqrwq5qk 215 14 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 215 15 RNA RNA NNP cord-298078-uqrwq5qk 215 16 ( ( -LRB- cord-298078-uqrwq5qk 215 17 59-GTCGACTTTAAACTGATACGGGGT 59-gtcgactttaaactgatacggggt CD cord-298078-uqrwq5qk 215 18 - - HYPH cord-298078-uqrwq5qk 215 19 ATC-39 ATC-39 NNP cord-298078-uqrwq5qk 215 20 and and CC cord-298078-uqrwq5qk 215 21 59-GAAGGATCCCAGCTGAAAGGC-39 59-GAAGGATCCCAGCTGAAAGGC-39 NNP cord-298078-uqrwq5qk 215 22 ) ) -RRB- cord-298078-uqrwq5qk 215 23 . . . cord-298078-uqrwq5qk 216 1 qRT qRT NNP cord-298078-uqrwq5qk 216 2 - - HYPH cord-298078-uqrwq5qk 216 3 PCR PCR NNP cord-298078-uqrwq5qk 216 4 was be VBD cord-298078-uqrwq5qk 216 5 performed perform VBN cord-298078-uqrwq5qk 216 6 with with IN cord-298078-uqrwq5qk 216 7 StepOne StepOne NNP cord-298078-uqrwq5qk 216 8 real real JJ cord-298078-uqrwq5qk 216 9 - - HYPH cord-298078-uqrwq5qk 216 10 time time NN cord-298078-uqrwq5qk 216 11 PCR pcr NN cord-298078-uqrwq5qk 216 12 system system NN cord-298078-uqrwq5qk 216 13 ( ( -LRB- cord-298078-uqrwq5qk 216 14 Applied Applied NNP cord-298078-uqrwq5qk 216 15 Biosystems Biosystems NNP cord-298078-uqrwq5qk 216 16 ) ) -RRB- cord-298078-uqrwq5qk 216 17 . . . cord-298078-uqrwq5qk 217 1 Reactions reaction NNS cord-298078-uqrwq5qk 217 2 were be VBD cord-298078-uqrwq5qk 217 3 amplified amplify VBN cord-298078-uqrwq5qk 217 4 using use VBG cord-298078-uqrwq5qk 217 5 selective selective JJ cord-298078-uqrwq5qk 217 6 primers primer NNS cord-298078-uqrwq5qk 217 7 as as IN cord-298078-uqrwq5qk 217 8 described describe VBN cord-298078-uqrwq5qk 217 9 above above IN cord-298078-uqrwq5qk 217 10 with with IN cord-298078-uqrwq5qk 217 11 Power Power NNP cord-298078-uqrwq5qk 217 12 SYBR SYBR NNP cord-298078-uqrwq5qk 217 13 master master NN cord-298078-uqrwq5qk 217 14 mix mix NN cord-298078-uqrwq5qk 217 15 ( ( -LRB- cord-298078-uqrwq5qk 217 16 Applied Applied NNP cord-298078-uqrwq5qk 217 17 Biosystems Biosystems NNP cord-298078-uqrwq5qk 217 18 ) ) -RRB- cord-298078-uqrwq5qk 217 19 according accord VBG cord-298078-uqrwq5qk 217 20 to to IN cord-298078-uqrwq5qk 217 21 the the DT cord-298078-uqrwq5qk 217 22 manufacturer manufacturer NN cord-298078-uqrwq5qk 217 23 's 's POS cord-298078-uqrwq5qk 217 24 instructions instruction NNS cord-298078-uqrwq5qk 217 25 . . . cord-298078-uqrwq5qk 218 1 Quantification quantification NN cord-298078-uqrwq5qk 218 2 was be VBD cord-298078-uqrwq5qk 218 3 carried carry VBN cord-298078-uqrwq5qk 218 4 out out RP cord-298078-uqrwq5qk 218 5 with with IN cord-298078-uqrwq5qk 218 6 the the DT cord-298078-uqrwq5qk 218 7 StepOne StepOne NNP cord-298078-uqrwq5qk 218 8 TM TM NNP cord-298078-uqrwq5qk 218 9 software software NN cord-298078-uqrwq5qk 218 10 ( ( -LRB- cord-298078-uqrwq5qk 218 11 Ver Ver NNP cord-298078-uqrwq5qk 218 12 2.2 2.2 CD cord-298078-uqrwq5qk 218 13 ) ) -RRB- cord-298078-uqrwq5qk 218 14 . . . cord-298078-uqrwq5qk 219 1 Percentages percentage NNS cord-298078-uqrwq5qk 219 2 of of IN cord-298078-uqrwq5qk 219 3 PK PK NNP cord-298078-uqrwq5qk 219 4 RNA RNA NNP cord-298078-uqrwq5qk 219 5 binding bind VBG cord-298078-uqrwq5qk 219 6 to to IN cord-298078-uqrwq5qk 219 7 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 219 8 were be VBD cord-298078-uqrwq5qk 219 9 expressed express VBN cord-298078-uqrwq5qk 219 10 as as IN cord-298078-uqrwq5qk 219 11 the the DT cord-298078-uqrwq5qk 219 12 _SP cord-298078-uqrwq5qk 219 13 To to TO cord-298078-uqrwq5qk 219 14 make make VB cord-298078-uqrwq5qk 219 15 IBV IBV NNP cord-298078-uqrwq5qk 219 16 dual dual JJ cord-298078-uqrwq5qk 219 17 luciferase luciferase NN cord-298078-uqrwq5qk 219 18 reporters reporter NNS cord-298078-uqrwq5qk 219 19 , , , cord-298078-uqrwq5qk 219 20 a a DT cord-298078-uqrwq5qk 219 21 frameshifting frameshifte VBG cord-298078-uqrwq5qk 219 22 sequence sequence NN cord-298078-uqrwq5qk 219 23 ( ( -LRB- cord-298078-uqrwq5qk 219 24 72 72 CD cord-298078-uqrwq5qk 219 25 bp bp NNP cord-298078-uqrwq5qk 219 26 ) ) -RRB- cord-298078-uqrwq5qk 219 27 was be VBD cord-298078-uqrwq5qk 219 28 amplified amplify VBN cord-298078-uqrwq5qk 219 29 from from IN cord-298078-uqrwq5qk 219 30 pFS pFS NNP cord-298078-uqrwq5qk 219 31 - - HYPH cord-298078-uqrwq5qk 219 32 cass cass NNP cord-298078-uqrwq5qk 219 33 5 5 CD cord-298078-uqrwq5qk 219 34 and and CC cord-298078-uqrwq5qk 219 35 pFS pFS NNP cord-298078-uqrwq5qk 219 36 - - HYPH cord-298078-uqrwq5qk 219 37 cass cass NNP cord-298078-uqrwq5qk 219 38 5.15 5.15 CD cord-298078-uqrwq5qk 219 39 . . . cord-298078-uqrwq5qk 220 1 The the DT cord-298078-uqrwq5qk 220 2 sequence sequence NN cord-298078-uqrwq5qk 220 3 was be VBD cord-298078-uqrwq5qk 220 4 inserted insert VBN cord-298078-uqrwq5qk 220 5 between between IN cord-298078-uqrwq5qk 220 6 Renilla Renilla NNP cord-298078-uqrwq5qk 220 7 and and CC cord-298078-uqrwq5qk 220 8 firefly firefly NN cord-298078-uqrwq5qk 220 9 luciferase luciferase NN cord-298078-uqrwq5qk 220 10 genes gene NNS cord-298078-uqrwq5qk 220 11 of of IN cord-298078-uqrwq5qk 220 12 a a DT cord-298078-uqrwq5qk 220 13 dual dual JJ cord-298078-uqrwq5qk 220 14 reporter reporter NN cord-298078-uqrwq5qk 220 15 , , , cord-298078-uqrwq5qk 220 16 p2Luc p2Luc NNP cord-298078-uqrwq5qk 220 17 vector vector NN cord-298078-uqrwq5qk 220 18 ( ( -LRB- cord-298078-uqrwq5qk 220 19 kindly kindly RB cord-298078-uqrwq5qk 220 20 provided provide VBN cord-298078-uqrwq5qk 220 21 by by IN cord-298078-uqrwq5qk 220 22 Dr. Dr. NNP cord-298078-uqrwq5qk 220 23 Yang Yang NNP cord-298078-uqrwq5qk 220 24 Kyun Kyun NNP cord-298078-uqrwq5qk 220 25 Kim Kim NNP cord-298078-uqrwq5qk 220 26 , , , cord-298078-uqrwq5qk 220 27 SungKyunKwan SungKyunKwan NNP cord-298078-uqrwq5qk 220 28 University University NNP cord-298078-uqrwq5qk 220 29 ) ) -RRB- cord-298078-uqrwq5qk 220 30 [ [ -LRB- cord-298078-uqrwq5qk 220 31 47 47 CD cord-298078-uqrwq5qk 220 32 ] ] -RRB- cord-298078-uqrwq5qk 220 33 . . . cord-298078-uqrwq5qk 221 1 Wildtype Wildtype NNP cord-298078-uqrwq5qk 221 2 reporter reporter NN cord-298078-uqrwq5qk 221 3 is be VBZ cord-298078-uqrwq5qk 221 4 composed compose VBN cord-298078-uqrwq5qk 221 5 of of IN cord-298078-uqrwq5qk 221 6 slippery slippery JJ cord-298078-uqrwq5qk 221 7 site site NN cord-298078-uqrwq5qk 221 8 , , , cord-298078-uqrwq5qk 221 9 stop stop VB cord-298078-uqrwq5qk 221 10 codon codon NN cord-298078-uqrwq5qk 221 11 , , , cord-298078-uqrwq5qk 221 12 spacer spacer NN cord-298078-uqrwq5qk 221 13 linker linker NN cord-298078-uqrwq5qk 221 14 and and CC cord-298078-uqrwq5qk 221 15 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 221 16 region region NN cord-298078-uqrwq5qk 221 17 of of IN cord-298078-uqrwq5qk 221 18 IBV IBV NNP cord-298078-uqrwq5qk 221 19 genomic genomic JJ cord-298078-uqrwq5qk 221 20 sequences sequence NNS cord-298078-uqrwq5qk 221 21 . . . cord-298078-uqrwq5qk 222 1 The the DT cord-298078-uqrwq5qk 222 2 mutant mutant JJ cord-298078-uqrwq5qk 222 3 reporter reporter NN cord-298078-uqrwq5qk 222 4 is be VBZ cord-298078-uqrwq5qk 222 5 as as RB cord-298078-uqrwq5qk 222 6 same same JJ cord-298078-uqrwq5qk 222 7 as as IN cord-298078-uqrwq5qk 222 8 the the DT cord-298078-uqrwq5qk 222 9 wild wild JJ cord-298078-uqrwq5qk 222 10 - - HYPH cord-298078-uqrwq5qk 222 11 type type NN cord-298078-uqrwq5qk 222 12 reporter reporter NN cord-298078-uqrwq5qk 222 13 except except IN cord-298078-uqrwq5qk 222 14 for for IN cord-298078-uqrwq5qk 222 15 a a DT cord-298078-uqrwq5qk 222 16 disrupted disrupt VBN cord-298078-uqrwq5qk 222 17 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 222 18 . . . cord-298078-uqrwq5qk 223 1 The the DT cord-298078-uqrwq5qk 223 2 in in IN cord-298078-uqrwq5qk 223 3 - - HYPH cord-298078-uqrwq5qk 223 4 frame frame NN cord-298078-uqrwq5qk 223 5 reporter reporter NN cord-298078-uqrwq5qk 223 6 has have VBZ cord-298078-uqrwq5qk 223 7 wild wild JJ cord-298078-uqrwq5qk 223 8 - - HYPH cord-298078-uqrwq5qk 223 9 type type NN cord-298078-uqrwq5qk 223 10 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 223 11 but but CC cord-298078-uqrwq5qk 223 12 not not RB cord-298078-uqrwq5qk 223 13 frameshifting frameshifte VBG cord-298078-uqrwq5qk 223 14 regulatory regulatory JJ cord-298078-uqrwq5qk 223 15 elements element NNS cord-298078-uqrwq5qk 223 16 , , , cord-298078-uqrwq5qk 223 17 where where WRB cord-298078-uqrwq5qk 223 18 no no DT cord-298078-uqrwq5qk 223 19 - - HYPH cord-298078-uqrwq5qk 223 20 frame frame NN cord-298078-uqrwq5qk 223 21 reporter reporter NN cord-298078-uqrwq5qk 223 22 has have VBZ cord-298078-uqrwq5qk 223 23 wild wild JJ cord-298078-uqrwq5qk 223 24 - - HYPH cord-298078-uqrwq5qk 223 25 type type NN cord-298078-uqrwq5qk 223 26 frameshifting frameshifte VBG cord-298078-uqrwq5qk 223 27 regulatory regulatory JJ cord-298078-uqrwq5qk 223 28 elements element NNS cord-298078-uqrwq5qk 223 29 except except IN cord-298078-uqrwq5qk 223 30 for for IN cord-298078-uqrwq5qk 223 31 the the DT cord-298078-uqrwq5qk 223 32 slippery slippery JJ cord-298078-uqrwq5qk 223 33 site site NN cord-298078-uqrwq5qk 223 34 . . . cord-298078-uqrwq5qk 224 1 Wild wild JJ cord-298078-uqrwq5qk 224 2 - - HYPH cord-298078-uqrwq5qk 224 3 type type NN cord-298078-uqrwq5qk 224 4 and and CC cord-298078-uqrwq5qk 224 5 mutant mutant JJ cord-298078-uqrwq5qk 224 6 IBV IBV NNP cord-298078-uqrwq5qk 224 7 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 224 8 sequences sequence NNS cord-298078-uqrwq5qk 224 9 were be VBD cord-298078-uqrwq5qk 224 10 amplified amplify VBN cord-298078-uqrwq5qk 224 11 by by IN cord-298078-uqrwq5qk 224 12 PCR PCR NNP cord-298078-uqrwq5qk 224 13 with with IN cord-298078-uqrwq5qk 224 14 primers primer NNS cord-298078-uqrwq5qk 224 15 59-AATGTCGACTT 59-aatgtcgactt CD cord-298078-uqrwq5qk 224 16 - - HYPH cord-298078-uqrwq5qk 224 17 TAAACTGATACGGG-39 taaactgatacggg-39 NN cord-298078-uqrwq5qk 224 18 and and CC cord-298078-uqrwq5qk 224 19 59-TAAGGATCCCAGCTGA 59-taaggatcccagctga CD cord-298078-uqrwq5qk 224 20 - - : cord-298078-uqrwq5qk 224 21 AAGGCTC-39 aaggctc-39 NN cord-298078-uqrwq5qk 224 22 from from IN cord-298078-uqrwq5qk 224 23 of of IN cord-298078-uqrwq5qk 224 24 pFS pFS NNP cord-298078-uqrwq5qk 224 25 - - HYPH cord-298078-uqrwq5qk 224 26 cass cass NNP cord-298078-uqrwq5qk 224 27 5 5 CD cord-298078-uqrwq5qk 224 28 and and CC cord-298078-uqrwq5qk 224 29 pFS pFS NNP cord-298078-uqrwq5qk 224 30 - - HYPH cord-298078-uqrwq5qk 224 31 cass cass NNP cord-298078-uqrwq5qk 224 32 5.15 5.15 CD cord-298078-uqrwq5qk 224 33 , , , cord-298078-uqrwq5qk 224 34 respectively respectively RB cord-298078-uqrwq5qk 224 35 . . . cord-298078-uqrwq5qk 225 1 In in IN cord-298078-uqrwq5qk 225 2 frame frame NN cord-298078-uqrwq5qk 225 3 sequence sequence NN cord-298078-uqrwq5qk 225 4 was be VBD cord-298078-uqrwq5qk 225 5 amplified amplify VBN cord-298078-uqrwq5qk 225 6 with with IN cord-298078-uqrwq5qk 225 7 primers primer NNS cord-298078-uqrwq5qk 225 8 59-AA 59-aa CD cord-298078-uqrwq5qk 225 9 - - HYPH cord-298078-uqrwq5qk 225 10 TGTCGACACGGGGTATCAGTC-39 tgtcgacacggggtatcagtc-39 NN cord-298078-uqrwq5qk 225 11 and and CC cord-298078-uqrwq5qk 225 12 59-TAAGGATCC 59-taaggatcc CD cord-298078-uqrwq5qk 225 13 - - HYPH cord-298078-uqrwq5qk 225 14 CAGCTGAAAGGCTC-39 cagctgaaaggctc-39 ADD cord-298078-uqrwq5qk 225 15 . . . cord-298078-uqrwq5qk 226 1 No no DT cord-298078-uqrwq5qk 226 2 frame frame NN cord-298078-uqrwq5qk 226 3 sequence sequence NN cord-298078-uqrwq5qk 226 4 was be VBD cord-298078-uqrwq5qk 226 5 amplified amplify VBN cord-298078-uqrwq5qk 226 6 with with IN cord-298078-uqrwq5qk 226 7 primers primer NNS cord-298078-uqrwq5qk 226 8 59-AATGTCGACCTGATACGGGGTAT-39 59-AATGTCGACCTGATACGGGGTAT-39 NNP cord-298078-uqrwq5qk 226 9 and and CC cord-298078-uqrwq5qk 226 10 59-TAAGGATCCCAGCTGAAAGGCTC-39 59-TAAGGATCCCAGCTGAAAGGCTC-39 NNP cord-298078-uqrwq5qk 226 11 . . . cord-298078-uqrwq5qk 227 1 Cells cell NNS cord-298078-uqrwq5qk 227 2 were be VBD cord-298078-uqrwq5qk 227 3 cultured culture VBN cord-298078-uqrwq5qk 227 4 and and CC cord-298078-uqrwq5qk 227 5 transfected transfecte VBN cord-298078-uqrwq5qk 227 6 with with IN cord-298078-uqrwq5qk 227 7 frameshifting frameshifte VBG cord-298078-uqrwq5qk 227 8 reporters reporter NNS cord-298078-uqrwq5qk 227 9 using use VBG cord-298078-uqrwq5qk 227 10 Lipofectamine Lipofectamine NNP cord-298078-uqrwq5qk 227 11 TM TM NNP cord-298078-uqrwq5qk 227 12 ( ( -LRB- cord-298078-uqrwq5qk 227 13 Invitrogen Invitrogen NNP cord-298078-uqrwq5qk 227 14 ) ) -RRB- cord-298078-uqrwq5qk 227 15 . . . cord-298078-uqrwq5qk 228 1 For for IN cord-298078-uqrwq5qk 228 2 the the DT cord-298078-uqrwq5qk 228 3 luciferase luciferase NN cord-298078-uqrwq5qk 228 4 assay assay NN cord-298078-uqrwq5qk 228 5 , , , cord-298078-uqrwq5qk 228 6 cells cell NNS cord-298078-uqrwq5qk 228 7 were be VBD cord-298078-uqrwq5qk 228 8 scraped scrape VBN cord-298078-uqrwq5qk 228 9 into into IN cord-298078-uqrwq5qk 228 10 100 100 CD cord-298078-uqrwq5qk 228 11 ml ml NNS cord-298078-uqrwq5qk 228 12 of of IN cord-298078-uqrwq5qk 228 13 passive passive JJ cord-298078-uqrwq5qk 228 14 lysis lysis NN cord-298078-uqrwq5qk 228 15 buffer buffer NN cord-298078-uqrwq5qk 228 16 ( ( -LRB- cord-298078-uqrwq5qk 228 17 Promega Promega NNP cord-298078-uqrwq5qk 228 18 ) ) -RRB- cord-298078-uqrwq5qk 228 19 . . . cord-298078-uqrwq5qk 229 1 Luciferase luciferase NN cord-298078-uqrwq5qk 229 2 activity activity NN cord-298078-uqrwq5qk 229 3 in in IN cord-298078-uqrwq5qk 229 4 the the DT cord-298078-uqrwq5qk 229 5 lysate lysate NN cord-298078-uqrwq5qk 229 6 was be VBD cord-298078-uqrwq5qk 229 7 determined determine VBN cord-298078-uqrwq5qk 229 8 with with IN cord-298078-uqrwq5qk 229 9 a a DT cord-298078-uqrwq5qk 229 10 dualluciferase dualluciferase NN cord-298078-uqrwq5qk 229 11 reporter reporter NN cord-298078-uqrwq5qk 229 12 assay assay NN cord-298078-uqrwq5qk 229 13 system system NN cord-298078-uqrwq5qk 229 14 ( ( -LRB- cord-298078-uqrwq5qk 229 15 Promega Promega NNP cord-298078-uqrwq5qk 229 16 ) ) -RRB- cord-298078-uqrwq5qk 229 17 , , , cord-298078-uqrwq5qk 229 18 according accord VBG cord-298078-uqrwq5qk 229 19 to to IN cord-298078-uqrwq5qk 229 20 the the DT cord-298078-uqrwq5qk 229 21 manufacturer manufacturer NN cord-298078-uqrwq5qk 229 22 's 's POS cord-298078-uqrwq5qk 229 23 instruction instruction NN cord-298078-uqrwq5qk 229 24 and and CC cord-298078-uqrwq5qk 229 25 measured measure VBN cord-298078-uqrwq5qk 229 26 with with IN cord-298078-uqrwq5qk 229 27 a a DT cord-298078-uqrwq5qk 229 28 Turner Turner NNP cord-298078-uqrwq5qk 229 29 Luminometer Luminometer NNP cord-298078-uqrwq5qk 229 30 TD-20/20 TD-20/20 NNP cord-298078-uqrwq5qk 229 31 . . . cord-298078-uqrwq5qk 230 1 Firefly Firefly NNP cord-298078-uqrwq5qk 230 2 luciferase luciferase NN cord-298078-uqrwq5qk 230 3 activity activity NN cord-298078-uqrwq5qk 230 4 was be VBD cord-298078-uqrwq5qk 230 5 normalized normalize VBN cord-298078-uqrwq5qk 230 6 to to IN cord-298078-uqrwq5qk 230 7 the the DT cord-298078-uqrwq5qk 230 8 activity activity NN cord-298078-uqrwq5qk 230 9 of of IN cord-298078-uqrwq5qk 230 10 renilla renilla NNP cord-298078-uqrwq5qk 230 11 luciferase luciferase NNP cord-298078-uqrwq5qk 230 12 . . . cord-298078-uqrwq5qk 231 1 Frameshifiting frameshifite VBG cord-298078-uqrwq5qk 231 2 efficiency efficiency NN cord-298078-uqrwq5qk 231 3 was be VBD cord-298078-uqrwq5qk 231 4 calculated calculate VBN cord-298078-uqrwq5qk 231 5 by by IN cord-298078-uqrwq5qk 231 6 the the DT cord-298078-uqrwq5qk 231 7 following following JJ cord-298078-uqrwq5qk 231 8 formula formula NN cord-298078-uqrwq5qk 231 9 : : : cord-298078-uqrwq5qk 231 10 where where WRB cord-298078-uqrwq5qk 231 11 Fe Fe NNP cord-298078-uqrwq5qk 231 12 = = SYM cord-298078-uqrwq5qk 231 13 Frameshifting Frameshifting NNP cord-298078-uqrwq5qk 231 14 efficiency efficiency NN cord-298078-uqrwq5qk 231 15 , , , cord-298078-uqrwq5qk 231 16 F F NNP cord-298078-uqrwq5qk 231 17 = = SYM cord-298078-uqrwq5qk 231 18 Wild wild JJ cord-298078-uqrwq5qk 231 19 - - HYPH cord-298078-uqrwq5qk 231 20 type type NN cord-298078-uqrwq5qk 231 21 or or CC cord-298078-uqrwq5qk 231 22 mutant mutant JJ cord-298078-uqrwq5qk 231 23 Luc Luc NNP cord-298078-uqrwq5qk 231 24 activity activity NN cord-298078-uqrwq5qk 231 25 , , , cord-298078-uqrwq5qk 231 26 Fn Fn NNP cord-298078-uqrwq5qk 231 27 = = SYM cord-298078-uqrwq5qk 231 28 No no DT cord-298078-uqrwq5qk 231 29 - - HYPH cord-298078-uqrwq5qk 231 30 frame frame NN cord-298078-uqrwq5qk 231 31 Luc Luc NNP cord-298078-uqrwq5qk 231 32 activity activity NN cord-298078-uqrwq5qk 231 33 and and CC cord-298078-uqrwq5qk 231 34 Fp Fp NNP cord-298078-uqrwq5qk 231 35 = = SYM cord-298078-uqrwq5qk 231 36 In in IN cord-298078-uqrwq5qk 231 37 - - HYPH cord-298078-uqrwq5qk 231 38 frame frame NN cord-298078-uqrwq5qk 231 39 Luc Luc NNP cord-298078-uqrwq5qk 231 40 activity activity NN cord-298078-uqrwq5qk 231 41 . . . cord-298078-uqrwq5qk 232 1 Programmed program VBN cord-298078-uqrwq5qk 232 2 ribosomal ribosomal NN cord-298078-uqrwq5qk 232 3 frameshifting frameshifte VBG cord-298078-uqrwq5qk 232 4 in in IN cord-298078-uqrwq5qk 232 5 HIV-1 HIV-1 NNP cord-298078-uqrwq5qk 232 6 and and CC cord-298078-uqrwq5qk 232 7 the the DT cord-298078-uqrwq5qk 232 8 SARS SARS NNP cord-298078-uqrwq5qk 232 9 - - HYPH cord-298078-uqrwq5qk 232 10 CoV CoV NNP cord-298078-uqrwq5qk 232 11 Viral Viral NNP cord-298078-uqrwq5qk 232 12 RNA RNA NNP cord-298078-uqrwq5qk 232 13 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 232 14 : : : cord-298078-uqrwq5qk 232 15 versatile versatile JJ cord-298078-uqrwq5qk 232 16 motifs motif NNS cord-298078-uqrwq5qk 232 17 in in IN cord-298078-uqrwq5qk 232 18 gene gene NN cord-298078-uqrwq5qk 232 19 expression expression NN cord-298078-uqrwq5qk 232 20 and and CC cord-298078-uqrwq5qk 232 21 replication replication NN cord-298078-uqrwq5qk 233 1 Decreasing decrease VBG cord-298078-uqrwq5qk 234 1 the the DT cord-298078-uqrwq5qk 234 2 frameshift frameshift NN cord-298078-uqrwq5qk 234 3 efficiency efficiency NN cord-298078-uqrwq5qk 234 4 translates translate VBZ cord-298078-uqrwq5qk 234 5 into into IN cord-298078-uqrwq5qk 234 6 an an DT cord-298078-uqrwq5qk 234 7 equivalent equivalent JJ cord-298078-uqrwq5qk 234 8 reduction reduction NN cord-298078-uqrwq5qk 234 9 of of IN cord-298078-uqrwq5qk 234 10 the the DT cord-298078-uqrwq5qk 234 11 replication replication NN cord-298078-uqrwq5qk 234 12 of of IN cord-298078-uqrwq5qk 234 13 the the DT cord-298078-uqrwq5qk 234 14 human human JJ cord-298078-uqrwq5qk 234 15 immunodeficiency immunodeficiency NN cord-298078-uqrwq5qk 234 16 virus virus NN cord-298078-uqrwq5qk 234 17 type type NN cord-298078-uqrwq5qk 234 18 1 1 CD cord-298078-uqrwq5qk 234 19 A a DT cord-298078-uqrwq5qk 234 20 three three CD cord-298078-uqrwq5qk 234 21 - - HYPH cord-298078-uqrwq5qk 234 22 stemmed stem VBN cord-298078-uqrwq5qk 234 23 mRNA mRNA NNP cord-298078-uqrwq5qk 234 24 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 234 25 in in IN cord-298078-uqrwq5qk 234 26 the the DT cord-298078-uqrwq5qk 234 27 SARS SARS NNP cord-298078-uqrwq5qk 234 28 coronavirus coronavirus NN cord-298078-uqrwq5qk 234 29 frameshift frameshift NN cord-298078-uqrwq5qk 234 30 signal signal NN cord-298078-uqrwq5qk 234 31 Trading trading NN cord-298078-uqrwq5qk 234 32 translation translation NN cord-298078-uqrwq5qk 234 33 with with IN cord-298078-uqrwq5qk 234 34 RNA rna NN cord-298078-uqrwq5qk 234 35 - - HYPH cord-298078-uqrwq5qk 234 36 binding bind VBG cord-298078-uqrwq5qk 234 37 proteins protein NNS cord-298078-uqrwq5qk 235 1 Translation translation NN cord-298078-uqrwq5qk 235 2 matters matter VBZ cord-298078-uqrwq5qk 235 3 : : : cord-298078-uqrwq5qk 236 1 protein protein NN cord-298078-uqrwq5qk 236 2 synthesis synthesis NN cord-298078-uqrwq5qk 236 3 defects defect NNS cord-298078-uqrwq5qk 236 4 in in IN cord-298078-uqrwq5qk 236 5 inherited inherit VBN cord-298078-uqrwq5qk 236 6 disease disease NN cord-298078-uqrwq5qk 237 1 An an DT cord-298078-uqrwq5qk 237 2 efficient efficient JJ cord-298078-uqrwq5qk 237 3 ribosomal ribosomal JJ cord-298078-uqrwq5qk 237 4 frame frame NN cord-298078-uqrwq5qk 237 5 - - HYPH cord-298078-uqrwq5qk 237 6 shifting shift VBG cord-298078-uqrwq5qk 237 7 signal signal NN cord-298078-uqrwq5qk 237 8 in in IN cord-298078-uqrwq5qk 237 9 the the DT cord-298078-uqrwq5qk 237 10 polymerase polymerase NN cord-298078-uqrwq5qk 237 11 - - HYPH cord-298078-uqrwq5qk 237 12 encoding encode VBG cord-298078-uqrwq5qk 237 13 region region NN cord-298078-uqrwq5qk 237 14 of of IN cord-298078-uqrwq5qk 237 15 the the DT cord-298078-uqrwq5qk 237 16 coronavirus coronavirus NN cord-298078-uqrwq5qk 237 17 IBV IBV NNP cord-298078-uqrwq5qk 237 18 Characterization characterization NN cord-298078-uqrwq5qk 237 19 of of IN cord-298078-uqrwq5qk 237 20 an an DT cord-298078-uqrwq5qk 237 21 efficient efficient JJ cord-298078-uqrwq5qk 237 22 coronavirus coronavirus NN cord-298078-uqrwq5qk 237 23 ribosomal ribosomal NN cord-298078-uqrwq5qk 237 24 frameshifting frameshifte VBG cord-298078-uqrwq5qk 237 25 signal signal NN cord-298078-uqrwq5qk 237 26 : : : cord-298078-uqrwq5qk 237 27 requirement requirement NN cord-298078-uqrwq5qk 237 28 for for IN cord-298078-uqrwq5qk 237 29 an an DT cord-298078-uqrwq5qk 237 30 RNA RNA NNP cord-298078-uqrwq5qk 237 31 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 237 32 Mutational Mutational NNP cord-298078-uqrwq5qk 237 33 analysis analysis NN cord-298078-uqrwq5qk 237 34 of of IN cord-298078-uqrwq5qk 237 35 the the DT cord-298078-uqrwq5qk 237 36 " " `` cord-298078-uqrwq5qk 237 37 slipperysequence slipperysequence NN cord-298078-uqrwq5qk 237 38 " " '' cord-298078-uqrwq5qk 237 39 component component NN cord-298078-uqrwq5qk 237 40 of of IN cord-298078-uqrwq5qk 237 41 a a DT cord-298078-uqrwq5qk 237 42 coronavirus coronavirus NN cord-298078-uqrwq5qk 237 43 ribosomal ribosomal JJ cord-298078-uqrwq5qk 237 44 frameshifting frameshifte VBG cord-298078-uqrwq5qk 237 45 signal signal NN cord-298078-uqrwq5qk 237 46 Structure structure NN cord-298078-uqrwq5qk 237 47 , , , cord-298078-uqrwq5qk 237 48 stability stability NN cord-298078-uqrwq5qk 237 49 and and CC cord-298078-uqrwq5qk 237 50 function function NN cord-298078-uqrwq5qk 237 51 of of IN cord-298078-uqrwq5qk 237 52 RNA rna NN cord-298078-uqrwq5qk 237 53 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 237 54 involved involve VBN cord-298078-uqrwq5qk 237 55 in in IN cord-298078-uqrwq5qk 237 56 stimulating stimulate VBG cord-298078-uqrwq5qk 237 57 ribosomal ribosomal JJ cord-298078-uqrwq5qk 237 58 frameshifting frameshifte VBG cord-298078-uqrwq5qk 237 59 Annexins Annexins NNP cord-298078-uqrwq5qk 237 60 -unique -unique NN cord-298078-uqrwq5qk 237 61 membrane membrane NN cord-298078-uqrwq5qk 237 62 binding bind VBG cord-298078-uqrwq5qk 237 63 proteins protein NNS cord-298078-uqrwq5qk 237 64 with with IN cord-298078-uqrwq5qk 237 65 diverse diverse JJ cord-298078-uqrwq5qk 237 66 functions function NNS cord-298078-uqrwq5qk 237 67 Annexin Annexin NNP cord-298078-uqrwq5qk 237 68 A2 A2 NNP cord-298078-uqrwq5qk 237 69 Is be VBZ cord-298078-uqrwq5qk 237 70 a a DT cord-298078-uqrwq5qk 237 71 Novel Novel NNP cord-298078-uqrwq5qk 237 72 RNA RNA NNP cord-298078-uqrwq5qk 237 73 - - HYPH cord-298078-uqrwq5qk 237 74 binding bind VBG cord-298078-uqrwq5qk 237 75 Protein Protein NNP cord-298078-uqrwq5qk 237 76 Annexin Annexin NNP cord-298078-uqrwq5qk 237 77 A2 A2 NNP cord-298078-uqrwq5qk 237 78 binds bind VBZ cord-298078-uqrwq5qk 237 79 to to IN cord-298078-uqrwq5qk 237 80 the the DT cord-298078-uqrwq5qk 237 81 localization localization NN cord-298078-uqrwq5qk 237 82 signal signal NN cord-298078-uqrwq5qk 237 83 in in IN cord-298078-uqrwq5qk 237 84 the the DT cord-298078-uqrwq5qk 237 85 39 39 CD cord-298078-uqrwq5qk 237 86 untranslated untranslated JJ cord-298078-uqrwq5qk 237 87 region region NN cord-298078-uqrwq5qk 237 88 of of IN cord-298078-uqrwq5qk 237 89 c c NNP cord-298078-uqrwq5qk 237 90 - - HYPH cord-298078-uqrwq5qk 237 91 myc myc NNP cord-298078-uqrwq5qk 237 92 mRNA mrna NN cord-298078-uqrwq5qk 237 93 Annexin Annexin NNP cord-298078-uqrwq5qk 237 94 A2 A2 NNP cord-298078-uqrwq5qk 238 1 recognises recognise VBZ cord-298078-uqrwq5qk 238 2 a a DT cord-298078-uqrwq5qk 238 3 specific specific JJ cord-298078-uqrwq5qk 238 4 region region NN cord-298078-uqrwq5qk 238 5 in in IN cord-298078-uqrwq5qk 238 6 the the DT cord-298078-uqrwq5qk 238 7 39-UTR 39-utr CD cord-298078-uqrwq5qk 238 8 of of IN cord-298078-uqrwq5qk 238 9 its -PRON- PRP$ cord-298078-uqrwq5qk 238 10 cognate cognate JJ cord-298078-uqrwq5qk 238 11 messenger messenger NN cord-298078-uqrwq5qk 238 12 RNA RNA NNP cord-298078-uqrwq5qk 238 13 Translational Translational NNP cord-298078-uqrwq5qk 238 14 Control Control NNP cord-298078-uqrwq5qk 238 15 of of IN cord-298078-uqrwq5qk 238 16 Collagen Collagen NNP cord-298078-uqrwq5qk 238 17 Prolyl Prolyl NNP cord-298078-uqrwq5qk 238 18 4-Hydroxylase-(I 4-hydroxylase-(i CD cord-298078-uqrwq5qk 238 19 ) ) -RRB- cord-298078-uqrwq5qk 239 1 Gene Gene NNP cord-298078-uqrwq5qk 239 2 Expression expression NN cord-298078-uqrwq5qk 239 3 under under IN cord-298078-uqrwq5qk 239 4 Hypoxia hypoxia NN cord-298078-uqrwq5qk 240 1 The the DT cord-298078-uqrwq5qk 240 2 mRNAbinding mrnabinde VBG cord-298078-uqrwq5qk 240 3 site site NN cord-298078-uqrwq5qk 240 4 of of IN cord-298078-uqrwq5qk 240 5 annexin annexin NNP cord-298078-uqrwq5qk 240 6 A2 A2 NNP cord-298078-uqrwq5qk 240 7 resides reside VBZ cord-298078-uqrwq5qk 240 8 in in IN cord-298078-uqrwq5qk 240 9 helices helix NNS cord-298078-uqrwq5qk 240 10 C c NN cord-298078-uqrwq5qk 240 11 - - HYPH cord-298078-uqrwq5qk 240 12 D D NNP cord-298078-uqrwq5qk 240 13 of of IN cord-298078-uqrwq5qk 240 14 its -PRON- PRP$ cord-298078-uqrwq5qk 240 15 domain domain NN cord-298078-uqrwq5qk 241 1 IV IV NNP cord-298078-uqrwq5qk 242 1 An an DT cord-298078-uqrwq5qk 242 2 AU AU NNP cord-298078-uqrwq5qk 242 3 - - HYPH cord-298078-uqrwq5qk 242 4 rich rich JJ cord-298078-uqrwq5qk 242 5 stem stem NN cord-298078-uqrwq5qk 242 6 - - HYPH cord-298078-uqrwq5qk 242 7 loop loop NN cord-298078-uqrwq5qk 242 8 structure structure NN cord-298078-uqrwq5qk 242 9 is be VBZ cord-298078-uqrwq5qk 242 10 a a DT cord-298078-uqrwq5qk 242 11 critical critical JJ cord-298078-uqrwq5qk 242 12 feature feature NN cord-298078-uqrwq5qk 242 13 of of IN cord-298078-uqrwq5qk 242 14 the the DT cord-298078-uqrwq5qk 242 15 perinuclear perinuclear NNP cord-298078-uqrwq5qk 242 16 localization localization NN cord-298078-uqrwq5qk 242 17 signal signal NN cord-298078-uqrwq5qk 242 18 of of IN cord-298078-uqrwq5qk 242 19 c c NNP cord-298078-uqrwq5qk 242 20 - - HYPH cord-298078-uqrwq5qk 242 21 myc myc NNP cord-298078-uqrwq5qk 242 22 mRNA mRNA NNP cord-298078-uqrwq5qk 242 23 Medicationrelated Medicationrelated NNP cord-298078-uqrwq5qk 242 24 problem problem NN cord-298078-uqrwq5qk 242 25 type type NN cord-298078-uqrwq5qk 242 26 and and CC cord-298078-uqrwq5qk 242 27 appearance appearance NN cord-298078-uqrwq5qk 242 28 rate rate NN cord-298078-uqrwq5qk 242 29 in in IN cord-298078-uqrwq5qk 242 30 ambulatory ambulatory JJ cord-298078-uqrwq5qk 242 31 hemodialysis hemodialysis NN cord-298078-uqrwq5qk 242 32 patients patient NNS cord-298078-uqrwq5qk 242 33 Annexin Annexin NNP cord-298078-uqrwq5qk 242 34 II II NNP cord-298078-uqrwq5qk 242 35 is be VBZ cord-298078-uqrwq5qk 242 36 associated associate VBN cord-298078-uqrwq5qk 242 37 with with IN cord-298078-uqrwq5qk 242 38 mRNAs mrnas XX cord-298078-uqrwq5qk 242 39 which which WDT cord-298078-uqrwq5qk 242 40 may may MD cord-298078-uqrwq5qk 242 41 constitute constitute VB cord-298078-uqrwq5qk 242 42 a a DT cord-298078-uqrwq5qk 242 43 distinct distinct JJ cord-298078-uqrwq5qk 242 44 subpopulation subpopulation NN cord-298078-uqrwq5qk 242 45 Loss loss NN cord-298078-uqrwq5qk 242 46 of of IN cord-298078-uqrwq5qk 242 47 Annexin Annexin NNP cord-298078-uqrwq5qk 242 48 II II NNP cord-298078-uqrwq5qk 242 49 Heavy Heavy NNP cord-298078-uqrwq5qk 242 50 and and CC cord-298078-uqrwq5qk 242 51 Light Light NNP cord-298078-uqrwq5qk 242 52 Chains Chains NNPS cord-298078-uqrwq5qk 242 53 in in IN cord-298078-uqrwq5qk 242 54 Prostate Prostate NNP cord-298078-uqrwq5qk 242 55 Cancer Cancer NNP cord-298078-uqrwq5qk 242 56 and and CC cord-298078-uqrwq5qk 242 57 Its -PRON- PRP$ cord-298078-uqrwq5qk 242 58 Precursors precursor NNS cord-298078-uqrwq5qk 242 59 Equal equal JJ cord-298078-uqrwq5qk 242 60 loading loading NN cord-298078-uqrwq5qk 242 61 of of IN cord-298078-uqrwq5qk 242 62 proteins protein NNS cord-298078-uqrwq5qk 242 63 were be VBD cord-298078-uqrwq5qk 242 64 confirmed confirm VBN cord-298078-uqrwq5qk 242 65 by by IN cord-298078-uqrwq5qk 242 66 anti anti JJ cord-298078-uqrwq5qk 242 67 - - JJ cord-298078-uqrwq5qk 242 68 b b JJ cord-298078-uqrwq5qk 242 69 - - HYPH cord-298078-uqrwq5qk 242 70 actin actin NN cord-298078-uqrwq5qk 242 71 antibody antibody NN cord-298078-uqrwq5qk 242 72 . . . cord-298078-uqrwq5qk 243 1 ( ( -LRB- cord-298078-uqrwq5qk 243 2 B b NN cord-298078-uqrwq5qk 243 3 ) ) -RRB- cord-298078-uqrwq5qk 244 1 Luciferase Luciferase NNP cord-298078-uqrwq5qk 244 2 reporter reporter NN cord-298078-uqrwq5qk 244 3 assay assay NN cord-298078-uqrwq5qk 244 4 after after IN cord-298078-uqrwq5qk 244 5 overexpression overexpression NN cord-298078-uqrwq5qk 244 6 of of IN cord-298078-uqrwq5qk 244 7 ANXA2 anxa2 NN cord-298078-uqrwq5qk 244 8 in in IN cord-298078-uqrwq5qk 244 9 LNCaP lncap NN cord-298078-uqrwq5qk 244 10 cells cell NNS cord-298078-uqrwq5qk 244 11 . . . cord-298078-uqrwq5qk 245 1 Relative relative JJ cord-298078-uqrwq5qk 245 2 frameshifting frameshifte VBG cord-298078-uqrwq5qk 245 3 efficiency efficiency NN cord-298078-uqrwq5qk 245 4 was be VBD cord-298078-uqrwq5qk 245 5 measured measure VBN cord-298078-uqrwq5qk 245 6 for for IN cord-298078-uqrwq5qk 245 7 the the DT cord-298078-uqrwq5qk 245 8 vector vector NN cord-298078-uqrwq5qk 245 9 and and CC cord-298078-uqrwq5qk 245 10 FLAG flag NN cord-298078-uqrwq5qk 245 11 - - HYPH cord-298078-uqrwq5qk 245 12 tagged tag VBN cord-298078-uqrwq5qk 245 13 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 245 14 transfected transfecte VBN cord-298078-uqrwq5qk 245 15 cells cell NNS cord-298078-uqrwq5qk 245 16 . . . cord-298078-uqrwq5qk 246 1 Expression expression NN cord-298078-uqrwq5qk 246 2 of of IN cord-298078-uqrwq5qk 246 3 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 246 4 was be VBD cord-298078-uqrwq5qk 246 5 confirmed confirm VBN cord-298078-uqrwq5qk 246 6 by by IN cord-298078-uqrwq5qk 246 7 Western western JJ cord-298078-uqrwq5qk 246 8 blot blot NN cord-298078-uqrwq5qk 246 9 analysis analysis NN cord-298078-uqrwq5qk 246 10 with with IN cord-298078-uqrwq5qk 246 11 anti anti JJ cord-298078-uqrwq5qk 246 12 - - JJ cord-298078-uqrwq5qk 246 13 ANXA2 anxa2 JJ cord-298078-uqrwq5qk 246 14 antibody antibody NN cord-298078-uqrwq5qk 246 15 . . . cord-298078-uqrwq5qk 247 1 Anti anti JJ cord-298078-uqrwq5qk 247 2 - - JJ cord-298078-uqrwq5qk 247 3 b b JJ cord-298078-uqrwq5qk 247 4 - - HYPH cord-298078-uqrwq5qk 247 5 actin actin NN cord-298078-uqrwq5qk 247 6 antibody antibody NN cord-298078-uqrwq5qk 247 7 was be VBD cord-298078-uqrwq5qk 247 8 used use VBN cord-298078-uqrwq5qk 247 9 as as IN cord-298078-uqrwq5qk 247 10 a a DT cord-298078-uqrwq5qk 247 11 loading loading NN cord-298078-uqrwq5qk 247 12 control control NN cord-298078-uqrwq5qk 247 13 . . . cord-298078-uqrwq5qk 248 1 ( ( -LRB- cord-298078-uqrwq5qk 248 2 C C NNP cord-298078-uqrwq5qk 248 3 ) ) -RRB- cord-298078-uqrwq5qk 248 4 Diagram Diagram NNP cord-298078-uqrwq5qk 248 5 of of IN cord-298078-uqrwq5qk 248 6 siRNA siRNA NNP cord-298078-uqrwq5qk 248 7 or or CC cord-298078-uqrwq5qk 248 8 shRNA shrna NN cord-298078-uqrwq5qk 248 9 target target NN cord-298078-uqrwq5qk 248 10 sequences sequence NNS cord-298078-uqrwq5qk 248 11 ( ( -LRB- cord-298078-uqrwq5qk 248 12 dashed dash VBN cord-298078-uqrwq5qk 248 13 line line NN cord-298078-uqrwq5qk 248 14 ) ) -RRB- cord-298078-uqrwq5qk 248 15 in in IN cord-298078-uqrwq5qk 248 16 the the DT cord-298078-uqrwq5qk 248 17 coding code VBG cord-298078-uqrwq5qk 248 18 regions region NNS cord-298078-uqrwq5qk 249 1 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 250 1 mRNA mRNA NNP cord-298078-uqrwq5qk 250 2 ( ( -LRB- cord-298078-uqrwq5qk 250 3 filled fill VBN cord-298078-uqrwq5qk 250 4 line line NN cord-298078-uqrwq5qk 250 5 ) ) -RRB- cord-298078-uqrwq5qk 250 6 . . . cord-298078-uqrwq5qk 251 1 Locations location NNS cord-298078-uqrwq5qk 251 2 of of IN cord-298078-uqrwq5qk 251 3 a a DT cord-298078-uqrwq5qk 251 4 shRNA shrna NN cord-298078-uqrwq5qk 251 5 and and CC cord-298078-uqrwq5qk 251 6 two two CD cord-298078-uqrwq5qk 251 7 siRNAs sirna NNS cord-298078-uqrwq5qk 251 8 were be VBD cord-298078-uqrwq5qk 251 9 indicated indicate VBN cord-298078-uqrwq5qk 251 10 ( ( -LRB- cord-298078-uqrwq5qk 251 11 shANX2 shanx2 NN cord-298078-uqrwq5qk 251 12 - - HYPH cord-298078-uqrwq5qk 251 13 1 1 CD cord-298078-uqrwq5qk 251 14 : : : cord-298078-uqrwq5qk 251 15 66 66 CD cord-298078-uqrwq5qk 251 16 - - SYM cord-298078-uqrwq5qk 251 17 83 83 CD cord-298078-uqrwq5qk 251 18 nt nt NN cord-298078-uqrwq5qk 251 19 , , , cord-298078-uqrwq5qk 251 20 siANX2 sianx2 NN cord-298078-uqrwq5qk 251 21 - - HYPH cord-298078-uqrwq5qk 251 22 2 2 CD cord-298078-uqrwq5qk 251 23 : : : cord-298078-uqrwq5qk 251 24 109 109 CD cord-298078-uqrwq5qk 251 25 - - SYM cord-298078-uqrwq5qk 251 26 129 129 CD cord-298078-uqrwq5qk 251 27 nt nt RB cord-298078-uqrwq5qk 251 28 , , , cord-298078-uqrwq5qk 251 29 siANX2 sianx2 NN cord-298078-uqrwq5qk 251 30 - - HYPH cord-298078-uqrwq5qk 251 31 3 3 CD cord-298078-uqrwq5qk 251 32 : : : cord-298078-uqrwq5qk 252 1 772 772 CD cord-298078-uqrwq5qk 252 2 - - SYM cord-298078-uqrwq5qk 252 3 792 792 CD cord-298078-uqrwq5qk 252 4 nt nt NN cord-298078-uqrwq5qk 252 5 ) ) -RRB- cord-298078-uqrwq5qk 252 6 . . . cord-298078-uqrwq5qk 253 1 ( ( -LRB- cord-298078-uqrwq5qk 253 2 D D NNP cord-298078-uqrwq5qk 253 3 ) ) -RRB- cord-298078-uqrwq5qk 253 4 Dual dual JJ cord-298078-uqrwq5qk 253 5 luciferase luciferase NN cord-298078-uqrwq5qk 253 6 reporter reporter NN cord-298078-uqrwq5qk 253 7 assay assay NN cord-298078-uqrwq5qk 253 8 following follow VBG cord-298078-uqrwq5qk 253 9 knockdown knockdown NN cord-298078-uqrwq5qk 253 10 of of IN cord-298078-uqrwq5qk 253 11 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 253 12 in in IN cord-298078-uqrwq5qk 253 13 HEK293 HEK293 NNP cord-298078-uqrwq5qk 253 14 T t NN cord-298078-uqrwq5qk 253 15 cells cell NNS cord-298078-uqrwq5qk 253 16 . . . cord-298078-uqrwq5qk 254 1 Relative relative JJ cord-298078-uqrwq5qk 254 2 frameshifting frameshifte VBG cord-298078-uqrwq5qk 254 3 efficiency efficiency NN cord-298078-uqrwq5qk 254 4 was be VBD cord-298078-uqrwq5qk 254 5 measured measure VBN cord-298078-uqrwq5qk 254 6 with with IN cord-298078-uqrwq5qk 254 7 pSUPER psuper NN cord-298078-uqrwq5qk 254 8 vector vector NN cord-298078-uqrwq5qk 254 9 ( ( -LRB- cord-298078-uqrwq5qk 254 10 Vector vector NN cord-298078-uqrwq5qk 254 11 ) ) -RRB- cord-298078-uqrwq5qk 254 12 and and CC cord-298078-uqrwq5qk 254 13 with with IN cord-298078-uqrwq5qk 254 14 pSUPER psuper NN cord-298078-uqrwq5qk 254 15 - - HYPH cord-298078-uqrwq5qk 254 16 ANXA2 anxa2 NN cord-298078-uqrwq5qk 254 17 ( ( -LRB- cord-298078-uqrwq5qk 254 18 shANX2 shanx2 NN cord-298078-uqrwq5qk 254 19 - - HYPH cord-298078-uqrwq5qk 254 20 1 1 CD cord-298078-uqrwq5qk 254 21 ) ) -RRB- cord-298078-uqrwq5qk 254 22 transfected transfecte VBN cord-298078-uqrwq5qk 254 23 cells cell NNS cord-298078-uqrwq5qk 254 24 . . . cord-298078-uqrwq5qk 255 1 Western western JJ cord-298078-uqrwq5qk 255 2 blot blot NN cord-298078-uqrwq5qk 255 3 analysis analysis NN cord-298078-uqrwq5qk 255 4 was be VBD cord-298078-uqrwq5qk 255 5 performed perform VBN cord-298078-uqrwq5qk 255 6 after after IN cord-298078-uqrwq5qk 255 7 shRNA shrna NN cord-298078-uqrwq5qk 255 8 transfection transfection NN cord-298078-uqrwq5qk 255 9 . . . cord-298078-uqrwq5qk 256 1 Anti anti JJ cord-298078-uqrwq5qk 256 2 - - JJ cord-298078-uqrwq5qk 256 3 b b JJ cord-298078-uqrwq5qk 256 4 - - HYPH cord-298078-uqrwq5qk 256 5 actin actin NN cord-298078-uqrwq5qk 256 6 antibody antibody NN cord-298078-uqrwq5qk 256 7 was be VBD cord-298078-uqrwq5qk 256 8 used use VBN cord-298078-uqrwq5qk 256 9 as as IN cord-298078-uqrwq5qk 256 10 a a DT cord-298078-uqrwq5qk 256 11 loading loading NN cord-298078-uqrwq5qk 256 12 control control NN cord-298078-uqrwq5qk 256 13 . . . cord-298078-uqrwq5qk 257 1 ( ( -LRB- cord-298078-uqrwq5qk 257 2 E E NNP cord-298078-uqrwq5qk 257 3 ) ) -RRB- cord-298078-uqrwq5qk 257 4 Luciferase Luciferase NNP cord-298078-uqrwq5qk 257 5 reporter reporter NN cord-298078-uqrwq5qk 257 6 assay assay NN cord-298078-uqrwq5qk 257 7 after after IN cord-298078-uqrwq5qk 257 8 knockdown knockdown NN cord-298078-uqrwq5qk 257 9 of of IN cord-298078-uqrwq5qk 257 10 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 257 11 in in IN cord-298078-uqrwq5qk 257 12 HEK293 HEK293 NNP cord-298078-uqrwq5qk 257 13 T t NN cord-298078-uqrwq5qk 257 14 cells cell NNS cord-298078-uqrwq5qk 257 15 . . . cord-298078-uqrwq5qk 258 1 Relative relative JJ cord-298078-uqrwq5qk 258 2 frameshifting frameshifte VBG cord-298078-uqrwq5qk 258 3 efficiency efficiency NN cord-298078-uqrwq5qk 258 4 was be VBD cord-298078-uqrwq5qk 258 5 measured measure VBN cord-298078-uqrwq5qk 258 6 from from IN cord-298078-uqrwq5qk 258 7 control control NN cord-298078-uqrwq5qk 258 8 siRNA siRNA NNP cord-298078-uqrwq5qk 258 9 ( ( -LRB- cord-298078-uqrwq5qk 258 10 siGFP siGFP NNP cord-298078-uqrwq5qk 258 11 ) ) -RRB- cord-298078-uqrwq5qk 258 12 or or CC cord-298078-uqrwq5qk 258 13 two two CD cord-298078-uqrwq5qk 258 14 independent independent JJ cord-298078-uqrwq5qk 258 15 ANXA2 ANXA2 NNP cord-298078-uqrwq5qk 258 16 siRNAs siRNAs NNP cord-298078-uqrwq5qk 258 17 ( ( -LRB- cord-298078-uqrwq5qk 258 18 siANX2 sianx2 NN cord-298078-uqrwq5qk 258 19 - - HYPH cord-298078-uqrwq5qk 258 20 2 2 CD cord-298078-uqrwq5qk 258 21 and and CC cord-298078-uqrwq5qk 258 22 -3 -3 NNP cord-298078-uqrwq5qk 258 23 ) ) -RRB- cord-298078-uqrwq5qk 258 24 transfected transfecte VBN cord-298078-uqrwq5qk 258 25 cells cell NNS cord-298078-uqrwq5qk 258 26 . . . cord-298078-uqrwq5qk 259 1 Western western JJ cord-298078-uqrwq5qk 259 2 blot blot NN cord-298078-uqrwq5qk 259 3 analysis analysis NN cord-298078-uqrwq5qk 259 4 was be VBD cord-298078-uqrwq5qk 259 5 performed perform VBN cord-298078-uqrwq5qk 259 6 after after IN cord-298078-uqrwq5qk 259 7 siRNA siRNA NNP cord-298078-uqrwq5qk 259 8 transfection transfection NN cord-298078-uqrwq5qk 259 9 . . . cord-298078-uqrwq5qk 260 1 Anti anti JJ cord-298078-uqrwq5qk 260 2 - - JJ cord-298078-uqrwq5qk 260 3 b b JJ cord-298078-uqrwq5qk 260 4 - - HYPH cord-298078-uqrwq5qk 260 5 actin actin NN cord-298078-uqrwq5qk 260 6 antibody antibody NN cord-298078-uqrwq5qk 260 7 was be VBD cord-298078-uqrwq5qk 260 8 used use VBN cord-298078-uqrwq5qk 260 9 as as IN cord-298078-uqrwq5qk 260 10 a a DT cord-298078-uqrwq5qk 260 11 loading loading NN cord-298078-uqrwq5qk 260 12 control control NN cord-298078-uqrwq5qk 260 13 pknotsRG pknotsRG NNP cord-298078-uqrwq5qk 260 14 : : : cord-298078-uqrwq5qk 260 15 RNA RNA NNP cord-298078-uqrwq5qk 260 16 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 260 17 folding fold VBG cord-298078-uqrwq5qk 260 18 including include VBG cord-298078-uqrwq5qk 260 19 near near JJ cord-298078-uqrwq5qk 260 20 - - HYPH cord-298078-uqrwq5qk 260 21 optimal optimal JJ cord-298078-uqrwq5qk 260 22 structures structure NNS cord-298078-uqrwq5qk 260 23 and and CC cord-298078-uqrwq5qk 260 24 sliding slide VBG cord-298078-uqrwq5qk 260 25 windows windows NNP cord-298078-uqrwq5qk 260 26 Design Design NNP cord-298078-uqrwq5qk 260 27 , , , cord-298078-uqrwq5qk 260 28 implementation implementation NN cord-298078-uqrwq5qk 260 29 and and CC cord-298078-uqrwq5qk 260 30 evaluation evaluation NN cord-298078-uqrwq5qk 260 31 of of IN cord-298078-uqrwq5qk 260 32 a a DT cord-298078-uqrwq5qk 260 33 piratical piratical JJ cord-298078-uqrwq5qk 260 34 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 260 35 folding folding NN cord-298078-uqrwq5qk 260 36 algorithm algorithm NN cord-298078-uqrwq5qk 260 37 based base VBN cord-298078-uqrwq5qk 260 38 on on IN cord-298078-uqrwq5qk 260 39 thermodynamics thermodynamic NNS cord-298078-uqrwq5qk 260 40 Structure structure NN cord-298078-uqrwq5qk 260 41 and and CC cord-298078-uqrwq5qk 260 42 function function NN cord-298078-uqrwq5qk 260 43 of of IN cord-298078-uqrwq5qk 260 44 telomerase telomerase NN cord-298078-uqrwq5qk 260 45 RNA RNA NNP cord-298078-uqrwq5qk 260 46 Structural structural JJ cord-298078-uqrwq5qk 260 47 basis basis NN cord-298078-uqrwq5qk 260 48 of of IN cord-298078-uqrwq5qk 260 49 a a DT cord-298078-uqrwq5qk 260 50 ribozyme ribozyme NN cord-298078-uqrwq5qk 260 51 's 's POS cord-298078-uqrwq5qk 260 52 thermostability thermostability NN cord-298078-uqrwq5qk 260 53 : : : cord-298078-uqrwq5qk 260 54 P1-L9 P1-L9 NNP cord-298078-uqrwq5qk 260 55 interdomain interdomain JJ cord-298078-uqrwq5qk 260 56 interaction interaction NN cord-298078-uqrwq5qk 260 57 in in IN cord-298078-uqrwq5qk 260 58 RNase RNase NNP cord-298078-uqrwq5qk 260 59 P P NNP cord-298078-uqrwq5qk 260 60 RNA RNA NNP cord-298078-uqrwq5qk 260 61 Folding Folding NNP cord-298078-uqrwq5qk 260 62 of of IN cord-298078-uqrwq5qk 260 63 the the DT cord-298078-uqrwq5qk 260 64 SAM SAM NNP cord-298078-uqrwq5qk 260 65 aptamer aptamer NNP cord-298078-uqrwq5qk 260 66 is be VBZ cord-298078-uqrwq5qk 260 67 determined determine VBN cord-298078-uqrwq5qk 260 68 by by IN cord-298078-uqrwq5qk 260 69 the the DT cord-298078-uqrwq5qk 260 70 formation formation NN cord-298078-uqrwq5qk 260 71 of of IN cord-298078-uqrwq5qk 260 72 a a DT cord-298078-uqrwq5qk 260 73 K k NN cord-298078-uqrwq5qk 260 74 - - HYPH cord-298078-uqrwq5qk 260 75 turn turn NN cord-298078-uqrwq5qk 260 76 - - HYPH cord-298078-uqrwq5qk 260 77 dependent dependent JJ cord-298078-uqrwq5qk 260 78 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 260 79 Endogenous endogenous JJ cord-298078-uqrwq5qk 260 80 expression expression NN cord-298078-uqrwq5qk 260 81 of of IN cord-298078-uqrwq5qk 260 82 a a DT cord-298078-uqrwq5qk 260 83 high high JJ cord-298078-uqrwq5qk 260 84 - - HYPH cord-298078-uqrwq5qk 260 85 affinity affinity NN cord-298078-uqrwq5qk 260 86 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 260 87 RNA RNA NNP cord-298078-uqrwq5qk 260 88 aptamer aptamer NNP cord-298078-uqrwq5qk 260 89 suppresses suppress VBZ cord-298078-uqrwq5qk 260 90 replication replication NN cord-298078-uqrwq5qk 260 91 of of IN cord-298078-uqrwq5qk 260 92 HIV-1 HIV-1 NNP cord-298078-uqrwq5qk 260 93 RNA RNA NNP cord-298078-uqrwq5qk 260 94 aptamers aptamer NNS cord-298078-uqrwq5qk 260 95 that that WDT cord-298078-uqrwq5qk 260 96 bind bind VBP cord-298078-uqrwq5qk 260 97 the the DT cord-298078-uqrwq5qk 260 98 nucleocapsid nucleocapsid NNP cord-298078-uqrwq5qk 260 99 protein protein NN cord-298078-uqrwq5qk 260 100 contain contain VBP cord-298078-uqrwq5qk 260 101 pseudoknots pseudoknot VBZ cord-298078-uqrwq5qk 260 102 Genetic genetic JJ cord-298078-uqrwq5qk 260 103 Interactions Interactions NNPS cord-298078-uqrwq5qk 260 104 between between IN cord-298078-uqrwq5qk 260 105 an an DT cord-298078-uqrwq5qk 260 106 Essential Essential NNP cord-298078-uqrwq5qk 260 107 39 39 CD cord-298078-uqrwq5qk 260 108 cis cis NNP cord-298078-uqrwq5qk 260 109 - - HYPH cord-298078-uqrwq5qk 260 110 Acting Acting NNP cord-298078-uqrwq5qk 260 111 RNA RNA NNP cord-298078-uqrwq5qk 260 112 Pseudoknot Pseudoknot NNP cord-298078-uqrwq5qk 260 113 , , , cord-298078-uqrwq5qk 260 114 Replicase Replicase NNP cord-298078-uqrwq5qk 260 115 Gene Gene NNP cord-298078-uqrwq5qk 260 116 Products Products NNP cord-298078-uqrwq5qk 260 117 , , , cord-298078-uqrwq5qk 260 118 and and CC cord-298078-uqrwq5qk 260 119 the the DT cord-298078-uqrwq5qk 260 120 Extreme Extreme NNP cord-298078-uqrwq5qk 260 121 39 39 CD cord-298078-uqrwq5qk 260 122 End end NN cord-298078-uqrwq5qk 260 123 of of IN cord-298078-uqrwq5qk 260 124 the the DT cord-298078-uqrwq5qk 260 125 Mouse Mouse NNP cord-298078-uqrwq5qk 260 126 Coronavirus Coronavirus NNP cord-298078-uqrwq5qk 260 127 Genome Genome NNP cord-298078-uqrwq5qk 260 128 Mutational Mutational NNP cord-298078-uqrwq5qk 260 129 study study NN cord-298078-uqrwq5qk 260 130 reveals reveal VBZ cord-298078-uqrwq5qk 260 131 that that IN cord-298078-uqrwq5qk 260 132 tertiary tertiary JJ cord-298078-uqrwq5qk 260 133 interactions interaction NNS cord-298078-uqrwq5qk 260 134 are be VBP cord-298078-uqrwq5qk 260 135 conserved conserve VBN cord-298078-uqrwq5qk 260 136 in in IN cord-298078-uqrwq5qk 260 137 ribosomal ribosomal JJ cord-298078-uqrwq5qk 260 138 frameshifting frameshifte VBG cord-298078-uqrwq5qk 260 139 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 260 140 of of IN cord-298078-uqrwq5qk 260 141 two two CD cord-298078-uqrwq5qk 260 142 luteoviruses luteoviruse NNS cord-298078-uqrwq5qk 260 143 Beyond beyond IN cord-298078-uqrwq5qk 260 144 Mfold Mfold NNP cord-298078-uqrwq5qk 260 145 : : : cord-298078-uqrwq5qk 260 146 recent recent JJ cord-298078-uqrwq5qk 260 147 advances advance NNS cord-298078-uqrwq5qk 260 148 in in IN cord-298078-uqrwq5qk 260 149 RNA RNA NNP cord-298078-uqrwq5qk 260 150 bioinformatics bioinformatics NNP cord-298078-uqrwq5qk 261 1 The the DT cord-298078-uqrwq5qk 261 2 MC MC NNP cord-298078-uqrwq5qk 261 3 - - HYPH cord-298078-uqrwq5qk 261 4 Fold Fold NNP cord-298078-uqrwq5qk 261 5 and and CC cord-298078-uqrwq5qk 261 6 MC MC NNP cord-298078-uqrwq5qk 261 7 - - HYPH cord-298078-uqrwq5qk 261 8 Sym Sym NNP cord-298078-uqrwq5qk 261 9 pipeline pipeline NN cord-298078-uqrwq5qk 261 10 infers infer VBZ cord-298078-uqrwq5qk 261 11 RNA RNA NNP cord-298078-uqrwq5qk 261 12 structure structure NN cord-298078-uqrwq5qk 261 13 from from IN cord-298078-uqrwq5qk 261 14 sequence sequence NN cord-298078-uqrwq5qk 261 15 data data NN cord-298078-uqrwq5qk 261 16 KnotSeeker KnotSeeker NNP cord-298078-uqrwq5qk 261 17 : : : cord-298078-uqrwq5qk 261 18 Heuristic heuristic JJ cord-298078-uqrwq5qk 261 19 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 261 20 detection detection NN cord-298078-uqrwq5qk 261 21 in in IN cord-298078-uqrwq5qk 261 22 long long JJ cord-298078-uqrwq5qk 261 23 RNA RNA NNP cord-298078-uqrwq5qk 261 24 sequences sequence NNS cord-298078-uqrwq5qk 262 1 The the DT cord-298078-uqrwq5qk 262 2 9-Å 9-å CD cord-298078-uqrwq5qk 262 3 solution solution NN cord-298078-uqrwq5qk 262 4 : : : cord-298078-uqrwq5qk 263 1 How how WRB cord-298078-uqrwq5qk 263 2 mRNA mrna NN cord-298078-uqrwq5qk 263 3 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 263 4 promote promote VBP cord-298078-uqrwq5qk 263 5 efficient efficient JJ cord-298078-uqrwq5qk 263 6 programmed program VBN cord-298078-uqrwq5qk 263 7 -1 -1 NN cord-298078-uqrwq5qk 263 8 ribosomal ribosomal JJ cord-298078-uqrwq5qk 263 9 frameshifting frameshifte VBG cord-298078-uqrwq5qk 263 10 Correlation correlation NN cord-298078-uqrwq5qk 263 11 between between IN cord-298078-uqrwq5qk 263 12 mechanical mechanical JJ cord-298078-uqrwq5qk 263 13 strength strength NN cord-298078-uqrwq5qk 263 14 of of IN cord-298078-uqrwq5qk 263 15 messenger messenger NN cord-298078-uqrwq5qk 263 16 RNA RNA NNP cord-298078-uqrwq5qk 263 17 pseudoknots pseudoknot NNS cord-298078-uqrwq5qk 263 18 and and CC cord-298078-uqrwq5qk 263 19 ribosomal ribosomal NN cord-298078-uqrwq5qk 264 1 frameshifting frameshifting NNP cord-298078-uqrwq5qk 265 1 A a DT cord-298078-uqrwq5qk 265 2 mechanical mechanical JJ cord-298078-uqrwq5qk 265 3 explanation explanation NN cord-298078-uqrwq5qk 265 4 of of IN cord-298078-uqrwq5qk 265 5 RNA RNA NNP cord-298078-uqrwq5qk 265 6 pseudoknot pseudoknot NNP cord-298078-uqrwq5qk 265 7 function function VB cord-298078-uqrwq5qk 265 8 in in IN cord-298078-uqrwq5qk 265 9 programmed program VBN cord-298078-uqrwq5qk 265 10 ribosomal ribosomal JJ cord-298078-uqrwq5qk 265 11 frameshifting frameshifte VBG cord-298078-uqrwq5qk 265 12 Ribosomal Ribosomal NNP cord-298078-uqrwq5qk 265 13 Pausing pause VBG cord-298078-uqrwq5qk 265 14 at at IN cord-298078-uqrwq5qk 265 15 a a DT cord-298078-uqrwq5qk 265 16 Frameshifter Frameshifter NNP cord-298078-uqrwq5qk 265 17 RNA RNA NNP cord-298078-uqrwq5qk 266 1 Pseudoknot Pseudoknot NNP cord-298078-uqrwq5qk 266 2 Is be VBZ cord-298078-uqrwq5qk 266 3 Sensitive sensitive JJ cord-298078-uqrwq5qk 266 4 to to IN cord-298078-uqrwq5qk 266 5 Reading reading NN cord-298078-uqrwq5qk 266 6 Phase phase NN cord-298078-uqrwq5qk 266 7 but but CC cord-298078-uqrwq5qk 266 8 Shows show VBZ cord-298078-uqrwq5qk 266 9 Little little JJ cord-298078-uqrwq5qk 266 10 Correlation correlation NN cord-298078-uqrwq5qk 266 11 with with IN cord-298078-uqrwq5qk 266 12 Frameshift Frameshift NNP cord-298078-uqrwq5qk 266 13 Efficiency Efficiency NNP cord-298078-uqrwq5qk 266 14 Isolation Isolation NNP cord-298078-uqrwq5qk 266 15 and and CC cord-298078-uqrwq5qk 266 16 Characterization characterization NN cord-298078-uqrwq5qk 266 17 of of IN cord-298078-uqrwq5qk 266 18 the the DT cord-298078-uqrwq5qk 266 19 102-Kilodalton 102-kilodalton CD cord-298078-uqrwq5qk 266 20 RNA RNA NNP cord-298078-uqrwq5qk 266 21 - - HYPH cord-298078-uqrwq5qk 266 22 binding bind VBG cord-298078-uqrwq5qk 266 23 Protein Protein NNP cord-298078-uqrwq5qk 266 24 That that WDT cord-298078-uqrwq5qk 266 25 Binds bind VBZ cord-298078-uqrwq5qk 266 26 to to IN cord-298078-uqrwq5qk 266 27 the the DT cord-298078-uqrwq5qk 266 28 5 5 CD cord-298078-uqrwq5qk 266 29 and and CC cord-298078-uqrwq5qk 266 30 3 3 CD cord-298078-uqrwq5qk 266 31 Translational Translational NNP cord-298078-uqrwq5qk 266 32 Enhancers Enhancers NNPS cord-298078-uqrwq5qk 266 33 of of IN cord-298078-uqrwq5qk 266 34 Tobacco Tobacco NNP cord-298078-uqrwq5qk 266 35 Mosaic Mosaic NNP cord-298078-uqrwq5qk 266 36 Virus Virus NNP cord-298078-uqrwq5qk 266 37 RNA RNA NNP cord-298078-uqrwq5qk 266 38 Eukaryotic Eukaryotic NNP cord-298078-uqrwq5qk 266 39 Elongation Elongation NNP cord-298078-uqrwq5qk 266 40 Factor Factor NNP cord-298078-uqrwq5qk 266 41 1A 1a CD cord-298078-uqrwq5qk 267 1 Interacts interact VBZ cord-298078-uqrwq5qk 267 2 with with IN cord-298078-uqrwq5qk 267 3 the the DT cord-298078-uqrwq5qk 267 4 Upstream Upstream NNP cord-298078-uqrwq5qk 267 5 Pseudoknot Pseudoknot NNP cord-298078-uqrwq5qk 267 6 Domain Domain NNP cord-298078-uqrwq5qk 267 7 in in IN cord-298078-uqrwq5qk 267 8 the the DT cord-298078-uqrwq5qk 267 9 39 39 CD cord-298078-uqrwq5qk 267 10 Untranslated Untranslated NNP cord-298078-uqrwq5qk 267 11 Region Region NNP cord-298078-uqrwq5qk 267 12 of of IN cord-298078-uqrwq5qk 267 13 Tobacco Tobacco NNP cord-298078-uqrwq5qk 267 14 Mosaic Mosaic NNP cord-298078-uqrwq5qk 267 15 Virus Virus NNP cord-298078-uqrwq5qk 267 16 RNA RNA NNP cord-298078-uqrwq5qk 267 17 Regulation Regulation NNP cord-298078-uqrwq5qk 267 18 of of IN cord-298078-uqrwq5qk 267 19 eukaryotic eukaryotic JJ cord-298078-uqrwq5qk 267 20 translation translation NN cord-298078-uqrwq5qk 267 21 by by IN cord-298078-uqrwq5qk 267 22 the the DT cord-298078-uqrwq5qk 267 23 RACK1 rack1 NN cord-298078-uqrwq5qk 267 24 protein protein NN cord-298078-uqrwq5qk 267 25 : : : cord-298078-uqrwq5qk 267 26 a a DT cord-298078-uqrwq5qk 267 27 platform platform NN cord-298078-uqrwq5qk 267 28 for for IN cord-298078-uqrwq5qk 267 29 signalling signal VBG cord-298078-uqrwq5qk 267 30 molecules molecule NNS cord-298078-uqrwq5qk 267 31 on on IN cord-298078-uqrwq5qk 267 32 the the DT cord-298078-uqrwq5qk 267 33 ribosome ribosome NN cord-298078-uqrwq5qk 268 1 The the DT cord-298078-uqrwq5qk 268 2 annexinopathies annexinopathie NNS cord-298078-uqrwq5qk 268 3 : : : cord-298078-uqrwq5qk 268 4 a a DT cord-298078-uqrwq5qk 268 5 new new JJ cord-298078-uqrwq5qk 268 6 category category NN cord-298078-uqrwq5qk 268 7 of of IN cord-298078-uqrwq5qk 268 8 diseases disease NNS cord-298078-uqrwq5qk 269 1 The the DT cord-298078-uqrwq5qk 269 2 role role NN cord-298078-uqrwq5qk 269 3 of of IN cord-298078-uqrwq5qk 269 4 annexin annexin NNP cord-298078-uqrwq5qk 269 5 II II NNP cord-298078-uqrwq5qk 269 6 in in IN cord-298078-uqrwq5qk 269 7 angiogenesis angiogenesis NN cord-298078-uqrwq5qk 269 8 and and CC cord-298078-uqrwq5qk 269 9 tumor tumor NN cord-298078-uqrwq5qk 269 10 progression progression NN cord-298078-uqrwq5qk 269 11 : : : cord-298078-uqrwq5qk 269 12 a a DT cord-298078-uqrwq5qk 269 13 potential potential JJ cord-298078-uqrwq5qk 269 14 therapeutic therapeutic JJ cord-298078-uqrwq5qk 269 15 target target NN cord-298078-uqrwq5qk 269 16 The the DT cord-298078-uqrwq5qk 269 17 Opitz Opitz NNP cord-298078-uqrwq5qk 269 18 syndrome syndrome NN cord-298078-uqrwq5qk 269 19 gene gene NN cord-298078-uqrwq5qk 269 20 product product NN cord-298078-uqrwq5qk 269 21 MID1 MID1 NNP cord-298078-uqrwq5qk 269 22 assembles assemble VBZ cord-298078-uqrwq5qk 269 23 a a DT cord-298078-uqrwq5qk 269 24 microtubule microtubule NN cord-298078-uqrwq5qk 269 25 - - HYPH cord-298078-uqrwq5qk 269 26 associated associate VBN cord-298078-uqrwq5qk 269 27 ribonucleoprotein ribonucleoprotein NN cord-298078-uqrwq5qk 269 28 complex complex JJ cord-298078-uqrwq5qk 269 29 Coronavirus Coronavirus NNP cord-298078-uqrwq5qk 269 30 Pathogenesis Pathogenesis NNP cord-298078-uqrwq5qk 269 31 and and CC cord-298078-uqrwq5qk 269 32 the the DT cord-298078-uqrwq5qk 269 33 Emerging Emerging NNP cord-298078-uqrwq5qk 269 34 Pathogen Pathogen NNP cord-298078-uqrwq5qk 269 35 Severe Severe NNP cord-298078-uqrwq5qk 269 36 Acute acute JJ cord-298078-uqrwq5qk 269 37 Respiratory Respiratory NNP cord-298078-uqrwq5qk 269 38 Syndrome Syndrome NNP cord-298078-uqrwq5qk 269 39 Coronavirus Coronavirus NNP cord-298078-uqrwq5qk 269 40 Identification Identification NNP cord-298078-uqrwq5qk 269 41 of of IN cord-298078-uqrwq5qk 269 42 novel novel JJ cord-298078-uqrwq5qk 269 43 ligands ligand NNS cord-298078-uqrwq5qk 269 44 for for IN cord-298078-uqrwq5qk 269 45 the the DT cord-298078-uqrwq5qk 269 46 RNA RNA NNP cord-298078-uqrwq5qk 269 47 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 269 48 that that WDT cord-298078-uqrwq5qk 269 49 regulate regulate VBP cord-298078-uqrwq5qk 269 50 -1 -1 CD cord-298078-uqrwq5qk 269 51 ribosomal ribosomal JJ cord-298078-uqrwq5qk 269 52 frameshifting frameshifte VBG cord-298078-uqrwq5qk 269 53 Specific specific JJ cord-298078-uqrwq5qk 269 54 inhibition inhibition NN cord-298078-uqrwq5qk 269 55 of of IN cord-298078-uqrwq5qk 269 56 gene gene NN cord-298078-uqrwq5qk 269 57 expression expression NN cord-298078-uqrwq5qk 269 58 using use VBG cord-298078-uqrwq5qk 269 59 a a DT cord-298078-uqrwq5qk 269 60 stably stably RB cord-298078-uqrwq5qk 269 61 integrated integrate VBN cord-298078-uqrwq5qk 269 62 , , , cord-298078-uqrwq5qk 269 63 inducible inducible JJ cord-298078-uqrwq5qk 269 64 small small RB cord-298078-uqrwq5qk 269 65 - - HYPH cord-298078-uqrwq5qk 269 66 interfering interfere VBG cord-298078-uqrwq5qk 269 67 - - HYPH cord-298078-uqrwq5qk 269 68 RNA rna NN cord-298078-uqrwq5qk 269 69 vector vector NN cord-298078-uqrwq5qk 269 70 b b NN cord-298078-uqrwq5qk 269 71 - - : cord-298078-uqrwq5qk 269 72 Catenin Catenin NNP cord-298078-uqrwq5qk 270 1 stabilizes stabilize VBZ cord-298078-uqrwq5qk 271 1 Cyclooxygenase-2 Cyclooxygenase-2 NNP cord-298078-uqrwq5qk 272 1 mRNA mrna NN cord-298078-uqrwq5qk 272 2 by by IN cord-298078-uqrwq5qk 272 3 interacting interact VBG cord-298078-uqrwq5qk 272 4 with with IN cord-298078-uqrwq5qk 272 5 AU AU NNP cord-298078-uqrwq5qk 272 6 - - HYPH cord-298078-uqrwq5qk 272 7 rich rich JJ cord-298078-uqrwq5qk 272 8 elements element NNS cord-298078-uqrwq5qk 272 9 of of IN cord-298078-uqrwq5qk 272 10 39-UTR 39-utr CD cord-298078-uqrwq5qk 272 11 From from IN cord-298078-uqrwq5qk 272 12 the the DT cord-298078-uqrwq5qk 272 13 Cover cover NN cord-298078-uqrwq5qk 272 14 : : : cord-298078-uqrwq5qk 272 15 Specific specific JJ cord-298078-uqrwq5qk 272 16 mutations mutation NNS cord-298078-uqrwq5qk 272 17 in in IN cord-298078-uqrwq5qk 272 18 a a DT cord-298078-uqrwq5qk 272 19 viral viral JJ cord-298078-uqrwq5qk 272 20 RNA RNA NNP cord-298078-uqrwq5qk 272 21 pseudoknot pseudoknot NN cord-298078-uqrwq5qk 272 22 drastically drastically RB cord-298078-uqrwq5qk 272 23 change change VB cord-298078-uqrwq5qk 272 24 ribosomal ribosomal JJ cord-298078-uqrwq5qk 272 25 frameshifting frameshifte VBG cord-298078-uqrwq5qk 272 26 efficiency efficiency NN cord-298078-uqrwq5qk 273 1 We -PRON- PRP cord-298078-uqrwq5qk 273 2 appreciate appreciate VBP cord-298078-uqrwq5qk 273 3 Drs Drs NNP cord-298078-uqrwq5qk 273 4 . . . cord-298078-uqrwq5qk 274 1 Brierly Brierly NNP cord-298078-uqrwq5qk 274 2 ( ( -LRB- cord-298078-uqrwq5qk 274 3 University University NNP cord-298078-uqrwq5qk 274 4 of of IN cord-298078-uqrwq5qk 274 5 Cambridge Cambridge NNP cord-298078-uqrwq5qk 274 6 ) ) -RRB- cord-298078-uqrwq5qk 274 7 , , , cord-298078-uqrwq5qk 274 8 Vedeler Vedeler NNP cord-298078-uqrwq5qk 274 9 ( ( -LRB- cord-298078-uqrwq5qk 274 10 University University NNP cord-298078-uqrwq5qk 274 11 of of IN cord-298078-uqrwq5qk 274 12 Bergen Bergen NNP cord-298078-uqrwq5qk 274 13 ) ) -RRB- cord-298078-uqrwq5qk 274 14 and and CC cord-298078-uqrwq5qk 274 15 Yang Yang NNP cord-298078-uqrwq5qk 274 16 Kyun Kyun NNP cord-298078-uqrwq5qk 274 17 Kim Kim NNP cord-298078-uqrwq5qk 274 18 ( ( -LRB- cord-298078-uqrwq5qk 274 19 SungKyunKwan SungKyunKwan NNP cord-298078-uqrwq5qk 274 20 University University NNP cord-298078-uqrwq5qk 274 21 ) ) -RRB- cord-298078-uqrwq5qk 274 22 for for IN cord-298078-uqrwq5qk 274 23 plasmids plasmid NNS cord-298078-uqrwq5qk 274 24 . . . cord-298078-uqrwq5qk 275 1 We -PRON- PRP cord-298078-uqrwq5qk 275 2 especially especially RB cord-298078-uqrwq5qk 275 3 thank thank VBP cord-298078-uqrwq5qk 275 4 Dr. Dr. NNP cord-298078-uqrwq5qk 275 5 Myeong Myeong NNP cord-298078-uqrwq5qk 275 6 - - HYPH cord-298078-uqrwq5qk 275 7 Hee Hee NNP cord-298078-uqrwq5qk 275 8 Yu Yu NNP cord-298078-uqrwq5qk 275 9 ( ( -LRB- cord-298078-uqrwq5qk 275 10 Director Director NNP cord-298078-uqrwq5qk 275 11 of of IN cord-298078-uqrwq5qk 275 12 Functional Functional NNP cord-298078-uqrwq5qk 275 13 Proteomics Proteomics NNP cord-298078-uqrwq5qk 275 14 Center Center NNP cord-298078-uqrwq5qk 275 15 , , , cord-298078-uqrwq5qk 275 16 Korea Korea NNP cord-298078-uqrwq5qk 275 17 Institute Institute NNP cord-298078-uqrwq5qk 275 18 of of IN cord-298078-uqrwq5qk 275 19 Science Science NNP cord-298078-uqrwq5qk 275 20 and and CC cord-298078-uqrwq5qk 275 21 Technology Technology NNP cord-298078-uqrwq5qk 275 22 ) ) -RRB- cord-298078-uqrwq5qk 275 23 for for IN cord-298078-uqrwq5qk 275 24 allowing allow VBG cord-298078-uqrwq5qk 275 25 us -PRON- PRP cord-298078-uqrwq5qk 275 26 to to TO cord-298078-uqrwq5qk 275 27 use use VB cord-298078-uqrwq5qk 275 28 proteomics proteomic NNS cord-298078-uqrwq5qk 275 29 facility facility NN cord-298078-uqrwq5qk 275 30 and and CC cord-298078-uqrwq5qk 275 31 providing provide VBG cord-298078-uqrwq5qk 275 32 us -PRON- PRP cord-298078-uqrwq5qk 275 33 excellent excellent JJ cord-298078-uqrwq5qk 275 34 analysis analysis NN cord-298078-uqrwq5qk 275 35 . . . cord-298078-uqrwq5qk 275 36 _SP