id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322516-wekvet6f	Maceyka, Michael	Ceramide Accumulation Uncovers a Cycling Pathway for the cis-Golgi Network Marker, Infectious Bronchitis Virus M Protein	1997-12-15		.txt	text/plain	6173	326	53	Short-term treatment with two inhibitors of sphingolipid synthesis had no effect on localization of IBV M or other Golgi markers. Treatment of cells with 100 M PDMP for 1 h had no effect on the localization of either protein (Fig. 4) , suggesting that the morphology of the Golgi was not greatly altered. Pretreatment with the earlier inhibitors reduced the PDMP-induced slowing of anterograde traffic by about half, suggesting that accumulation of newly synthesized ceramide at least partially mediates this effect. The pretreatment experiments suggested that increased levels of ceramide mediate the PDMP-induced effects on IBV M localization. However, neither ␤CA nor FB1 had an effect on the rate of anterograde traffic of VSV G or the localization of IC, CGN, or Golgi stack proteins. However, the glucosylceramide analogue PDMP, at a concentration that inhibits both GlcCer and SM synthases, causes a redistribution of IBV M to the ER and a slowing of anterograde traffic in BHK-21 cells.	./cache/cord-322516-wekvet6f.txt	./txt/cord-322516-wekvet6f.txt