id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308950-bl83r4v3	Miguel, B.	The role of feline aminopeptidase N as a receptor for infectious bronchitis virus : Brief Review	2002		.txt	text/plain	2543	157	56	Our objective was to determine if fAPN can serve as a receptor for infectious bronchitis virus (IBV). Feline kidney cells that express fAPN and hamster kidney fibroblasts that do not express fAPN were inoculated with IBV and monitored for replication by indirect fluorescent assay and confocal microscopy and in chicken embryonated eggs. To evaluate the permissiveness of cells to IBV, monolayers of transfected (pBK-fAPN and pBK-CMV) BHK-21, non-transfected BHK-21 and FEK on coverslips were infected with a 10 3.2 EID 50 of Ark/IBV virus. To test the hypothesis that the feline APN had a role in permissiveness of FEK cells to Ark/IBV, BHK-21 cells were transfected with pBK-CMV plasmid or pBK-fAPN. The BHK-21 cells transfected with pBK-fAPN were shown to express the fAPN receptor on the cell surface (Fig. 3) , and were permissive to Ark/IBV (Figs.	./cache/cord-308950-bl83r4v3.txt	./txt/cord-308950-bl83r4v3.txt