id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305079-foifc8ch	Zhou, Ying Shun	Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120	2013-02-22		.txt	text/plain	4842	229	54	The remaining one third of the genome encodes the structural proteins and group-specific ORFs, including spike glycoprotein (S), envelope protein (E), membrane Veterinary Microbiology 162 (2013) Infectious bronchitis virus (IBV) strain H120 was successfully rescued as infectious clone by reverse genetics. In this study, we describe the in vitro assembly and recovery of an infectious clone of IBV-H120, the biological and immune characteristics of the rescued H120 virus (R-H120), and the potential to use R120 as a candidate of vaccine vector in the future vaccine development. The IBV strains, H120 and Mass41, obtained from China Institute of Veterinary Drug Control (IVDC), were propagated in the allantoic cavities of the 10-day-old specific pathogen-free (SPF) embryonated chicken eggs (ECE), and the allantoic fluid was harvested 36 h post inoculation. Three groups of chickens were challenged with the virulent strain Mass41 to test if the rescued virus could protect the immunized animals from IBV infection.	./cache/cord-305079-foifc8ch.txt	./txt/cord-305079-foifc8ch.txt