id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-032753-icteg34y	Namai, Fu	Microbial therapeutics for acute colitis based on genetically modified Lactococcus lactis hypersecreting IL-1Ra in mice	2020-09-28		.txt	text/plain	5311	313	51	Oral administration of these gmLAB suppressed body weight reduction and exacerbation of the disease activity index score in mice with acute colitis and decreased the number of CD4(+) IL-17A(+) cells in the mesenteric lymph nodes. HE staining of colon sections removed on the last day revealed a decrease in immune cell infiltration in the NZ-IL1Ra administration group and a significant decrease in the total mucosal tissue area (Fig. 5e-g) . Production of IL-2 by EL4.NOB-1 cells stimulated with IL-1β was suppressed by increasing the concentration of rmIL-1Ra purified from NZ-IL1Ra supernatant, commercially available mIL-1Ra, or hIL-1Ra. These results indicate that rmIL-1Ra produced by gmLAB can play an effective role as an endogenous antagonist of IL-1. In conclusion, we successfully constructed gmLAB that hypersecrete bioactive mIL-1Ra. Oral administration of NZ-IL1Ra to acute colitis mice alleviated colitis symptoms and suppressed excessive immune reactions in the intestinal tract and MLNs. These results suggest that rmIL-1Ra reaches the colon via NZ-IL1Ra and inhibits IL-1 signaling.	./cache/cord-032753-icteg34y.txt	./txt/cord-032753-icteg34y.txt