id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301935-0qjo94ty	Varma, Ratna	Current strategies and opportunities to manufacture cells for modeling human lungs	2020-08-22		.txt	text/plain	10320	515	35	Current lung directed differentiation protocols are limited as they do not 1) recapitulate the diversity of respiratory epithelium, 2) generate consistent or sufficient cell numbers for drug discovery platforms, and 3) establish the histologic tissue-level organization critical for modeling lung function. We then discuss the evolution of directed differentiation protocols to find opportunities for creating specific populations of airway and lung epithelia through targeted manipulation of key signaling pathways in 2D and 3D models. While the cell fate of early proximal and distal lineages is directed through chemical signals, the lung epithelium itself undergoes marked changes in architecture, a process known as branching morphogenesis [79, 92] . In the future, human PSC-derived lung tissue models have the potential to enable exploration of infection, disease and regeneration mechanisms of action to impact drug discovery and drug development, and further inform patient-specific drug selection. Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein	./cache/cord-301935-0qjo94ty.txt	./txt/cord-301935-0qjo94ty.txt