id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267149-5twx9y5c	Abraham, Jonathan	Host-Species Transferrin Receptor 1 Orthologs Are Cellular Receptors for Nonpathogenic New World Clade B Arenaviruses	2009-04-03		.txt	text/plain	5396	287	55	The ability of a New World (NW) clade B arenavirus to enter cells using human transferrin receptor 1 (TfR1) strictly correlates with its ability to cause hemorrhagic fever. Here we show that pseudotyped viruses bearing the surface glycoprotein (GP) of AMAV or TCRV can infect cells using the TfR1 orthologs of several mammalian species, including those of their respective natural hosts, the small rodent Neacomys spinosus and the fruit bat Artibeus jamaicensis. In order to determine the ability of the AMAV and TCRV GP1 proteins to bind these TfR1 orthologs, HEK293T cells were transfected with plasmids expressing human, Ns, or AjTfR1. As the pathogenic NW arenaviruses efficiently enter HEK293T cells using endogenous human TfR1 [32] , CHO cells were transfected with plasmids expressing human TfR1, AjTfR1, NsTfR1, ZbTfR1, or vector alone, and subsequently infected with MACV, JUNV, or GTOV pseudoviruses.	./cache/cord-267149-5twx9y5c.txt	./txt/cord-267149-5twx9y5c.txt