id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336713-if6f58ii	Yuan, Lijuan	Short-term immunoglobulin A B-cell memory resides in intestinal lymphoid tissues but not in bone marrow of gnotobiotic pigs inoculated with Wa human rotavirus	2001-06-01		.txt	text/plain	7543	367	56	To investigate the development and sites of residence of intestinal memory B cells, and their role in protective immunity to reinfection with an enteric virus, we assessed the association between memory B cell and antibody-secreting cell (ASC) responses and protection using a gnotobiotic pig model for human rotavirus (HRV) infection and diarrhoea. The isotypes, quantities and tissue distribution of rotavirus-specific memory B cells and ASC were evaluated prechallenge (28 and 83 postinoculation days [PID]) and postchallenge (7 postchallenge days [PCD]), using enzyme-linked immunospot (ELISPOT) assay, in gnotobiotic pigs inoculated once with virulent or three times with attenuated HRV and challenged at PID 28 with the corresponding virulent HRV. In previous studies, 3, 4 we reported that the number of rotavirus-speci®c immunoglobulin A (IgA) antibody-secreting cells (ASC) present in the intestinal lamina propria of gnotobiotic pigs at the time of challenge (primary ASC) correlates with protection against infection and diarrhoea when challenged with human rotavirus (HRV).	./cache/cord-336713-if6f58ii.txt	./txt/cord-336713-if6f58ii.txt