id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-327344-8gi1wb76	Gambarino, Stefano	Development of a RT Real-Time PCR for the Detection and Quantification of Human Rhinoviruses	2009-03-17		.txt	text/plain	3506	147	38	This article describes the development and optimization of a reverse transcription (RT) real-time PCR assay for quantification of HRV RNA in clinical samples. Clinical specimens originated from the Virology Unit of the Fig. 1 Standard curve (from 10 2 to 10 5 copies/reaction) and dynamic range (from 10 7 to 10 1 copies/reaction) of the real-time RT-PCR developed in this study Azienda Ospedaliero-Universitaria San Giovanni Battista, Turin, and included 110 bronchoalveolar lavages (BAL) obtained from 84 patients (M/F, 57/27; mean age, 57.8 years; range, . The performance of the RT real-time PCR developed in this study was examined over different concentrations of HRV RNA and it was found to be very sensitive with a minimum cut-off for detection of 10 0 copy/reaction and was linear up to 10 1 copies. In conclusion, the RT real-time PCR assay developed in this study could represent a useful tool for diagnosing HRV infections, quantifying the viral load and could be applicable for routine diagnostic workup of upper as well as lower respiratory tract diseases.	./cache/cord-327344-8gi1wb76.txt	./txt/cord-327344-8gi1wb76.txt