id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-009922-t1hoox6e	Dearden, C. J.	Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay	2005-12-07		.txt	text/plain	4065	205	55	This paper describes the first enzyme‐linked immunosorbent assay for the detection of rhinovirus antigens in clinical specimens (nasal washings), either directly or following overnight cell culture amplification. Secondly, a direct ELISA system was developed in which the nasal washings or control antigen (uninfected tissue culture fluid) were added directly to each of a set of duplicate ELISA plate wells coated with either pre-or postchallenge rabbit anti HRV-EL hyperimmune serum. Figure 2 shows the results obtained with nasal washings, collected from three volunteers on consecutive days following HKV-EL or saline challenge, tested in both the cell-culture-amplified (CCA)-ELISA and direct ELISA systems. Although we would like to emphasise that our data are preliminary, both the direct and CCA-ELISAs gave a good correlation with virus isolation when used to detect rhinovirus antigens in nasal washings (obtained from 18 volunteers challenged with either HRV-EL or saline).	./cache/cord-009922-t1hoox6e.txt	./txt/cord-009922-t1hoox6e.txt