id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-343262-zopxdw1d	Srinivasan, Raghuraman C.	Effects of Cryogenic Storage on Human Amnion Epithelial Cells	2020-07-15		.txt	text/plain	5436	283	42	In this study, hAEC were isolated from full-term human placenta from 14 different donors, cryopreserved using a protocol and reagents commonly adopted for epithelial cell preservation. In anticipation that the transplant product used in any future clinical trial will be previously cryopreserved cells, the current study was undertaken to determine if there are any significant differences between the populations initially isolated from the amnion membranes, and the cells recovered following the cryopreservation procedure. The presence of CD45, a receptor linked to protein tyrosine phosphatase present in cells of the hematopoietic lineage, was detected in 9/12 of hAEC preparations immediately after isolation at an average level of 1.2% ± 1.4% cells, but greatly reduced after cryopreservation and washing steps, where only four preparations still contained an extremely low number of CD45 positive cells (0.3% ± 0.4%; p = 0.0146) ( Figure 2D ). The presence of HLA-G on hAEC after isolation was confirmed using a specific antibody, and HLA-G Amnion epithelial cells have been reported to express characteristic immunomodulatory molecules, such as HLA class Ib [21, 22] .	./cache/cord-343262-zopxdw1d.txt	./txt/cord-343262-zopxdw1d.txt