key: cord-013481-3zwq67do authors: Guo, Kejun; Shen, Guannan; Kibbie, Jon; Gonzalez, Tania; Dillon, Stephanie M.; Smith, Harry A.; Cooper, Emily H.; Lavender, Kerry; Hasenkrug, Kim J.; Sutter, Kathrin; Dittmer, Ulf; Kroehl, Miranda; Kechris, Katerina; Wilson, Cara C.; Santiago, Mario L. title: Qualitative Differences Between the IFNα subtypes and IFNβ Influence Chronic Mucosal HIV-1 Pathogenesis date: 2020-10-16 journal: PLoS Pathog DOI: 10.1371/journal.ppat.1008986 sha: doc_id: 13481 cord_uid: 3zwq67do The Type I Interferons (IFN-Is) are innate antiviral cytokines that include 12 different IFNα subtypes and IFNβ that signal through the IFN-I receptor (IFNAR), inducing hundreds of IFN-stimulated genes (ISGs) that comprise the ‘interferome’. Quantitative differences in IFNAR binding correlate with antiviral activity, but whether IFN-Is exhibit qualitative differences remains controversial. Moreover, the IFN-I response is protective during acute HIV-1 infection, but likely pathogenic during the chronic stages. To gain a deeper understanding of the IFN-I response, we compared the interferomes of IFNα subtypes dominantly-expressed in HIV-1-exposed plasmacytoid dendritic cells (1, 2, 5, 8 and 14) and IFNβ in the earliest cellular targets of HIV-1 infection. Primary gut CD4 T cells from 3 donors were treated for 18 hours ex vivo with individual IFN-Is normalized for IFNAR signaling strength. Of 1,969 IFN-regulated genes, 246 ‘core ISGs’ were induced by all IFN-Is tested. However, many IFN-regulated genes were not shared between the IFNα subtypes despite similar induction of canonical antiviral ISGs such as ISG15, RSAD2 and MX1, formally demonstrating qualitative differences between the IFNα subtypes. Notably, IFNβ induced a broader interferome than the individual IFNα subtypes. Since IFNβ, and not IFNα, is upregulated during chronic HIV-1 infection in the gut, we compared core ISGs and IFNβ-specific ISGs from colon pinch biopsies of HIV-1-uninfected (n = 13) versus age- and gender-matched, antiretroviral-therapy naïve persons with HIV-1 (PWH; n = 19). Core ISGs linked to inflammation, T cell activation and immune exhaustion were elevated in PWH, positively correlated with plasma lipopolysaccharide (LPS) levels and gut IFNβ levels, and negatively correlated with gut CD4 T cell frequencies. In sharp contrast, IFNβ-specific ISGs linked to protein translation and anti-inflammatory responses were significantly downregulated in PWH, negatively correlated with gut IFNβ and LPS, and positively correlated with plasma IL6 and gut CD4 T cell frequencies. Our findings reveal qualitative differences in interferome induction by diverse IFN-Is and suggest potential mechanisms for how IFNβ may drive HIV-1 pathogenesis in the gut. a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 The type I interferons (IFN-Is) are innate antiviral cytokines that include IFNα (12 different subtypes) and IFNβ [1] . These cytokines significantly inhibited HIV-1 replication in vitro, but human clinical trials with IFNα2 showed only moderate or no inhibitory effects on HIV-1 infection [2, 3] . All IFN-Is bind to the same IFN-I receptor that is composed of two subunits, IFNAR1 and IFNAR2, resulting in phosphorylation of JAK1 and TYK2. This in turn results in the phosphorylation of STAT1 and STAT2 that associate with IRF9 to form the transcriptional activator, ISGF3 [4] . ISGF3 translocates to the nucleus, where it binds to promoters of genes encoding IFN response elements (ISREs), resulting in the induction of hundreds of IFN stimulated genes (ISGs), collectively referred to as the 'interferome' [5] . Recent studies highlighted IFNα as a potential adjunct to an HIV-1 curative strategy [6-10]. However, almost all HIV-1 clinical trials with IFNα were performed with only one subtype, IFNα2, with mixed results (reviewed in [2, 3] ). More recently, IFNα2 treatment of SIV rhesus macaques under antiretroviral therapy did not reduce the latent HIV-1 reservoir [11] . Our group and others reported that IFNα2 only moderately inhibited HIV-1 in humanized mice and primary CD4+ T cells compared to the more potent subtypes IFNα6, IFNα14 and IFNα8 [12] [13] [14] [15] . Interestingly, the anti-HIV-1 potency of IFNα subtypes correlated with their binding affinity to IFNAR2, suggesting that the antiviral differences between the IFNα subtypes were due to quantitative differences in IFNAR signaling strength [12, 15, 16] . However, several human IFNα subtypes exhibit strong signals of purifying selection [17] , suggesting that these IFNα subtypes may have evolved to have specific, nonredundant functions. Some IFNα subtypes were better at inducing certain immune responses in vivo [13, 18] and may trigger distinct intracellular signaling pathways [19] . Nevertheless, the notion of qualitative differences between IFNα subtypes remains controversial [20, 21] . One reason is that most comparative studies normalized IFN-Is using protein amounts or Units/ml based on inhibition of vesicular stomatitis virus or encephalomyocarditis virus [1] . To unravel qualitative differences between the IFN-Is, it would be important to normalize IFN-Is for quantitative differences in IFNAR signaling strength. It is widely accepted that IFN-I signaling can prevent acute retrovirus infection. Genetic ablation of IFNAR resulted in higher Friend retrovirus replication in mice co-infected with lactate-dehydrogenase elevating virus, a potent IFN-I inducer [22] . Moreover, IFNAR blockade increased SIV replication in rhesus macaques [23, 24] . Administration of IFNα2 decreased retrovirus replication in mice, monkeys and humans [3, 18, 23] . However, in persistent virus infections, chronic IFN-I stimulation was linked to pathogenic outcomes [25] [26] [27] [28] [29] . Although clinical administration of IFNα2 increased the number of low-dose mucosal inoculations needed for breakthrough SIV infection in rhesus macaques, once the infection was established, lower CD4 T cell counts were observed in IFNα2-treated monkeys relative to untreated controls [23] . These findings highlight that HIV-1 infection shares features with 'interferonopathies' such as Aicardi-Goutières Syndrome [30] , which are currently being targeted through IFN-I blockade strategies (clinicaltrial.gov NCT03921554). In fact, IFNAR blockade during chronic HIV-1 infection in humanized mice restored immune function, leading to better HIV-1 control [31, 32] . Neutralization of (most) IFNα subtypes in SIV-infected rhesus macaques prior to infection increased viral loads as expected, but also decreased subsequent immune activation profiles [24] . By contrast, blockade of IFN-I signaling in chronic SIVtreated and untreated rhesus macaques decreased inflammation profiles associated with ISGs but did not reverse T cell exhaustion or activation [33] . The basis for the protective versus pathogenic effects of IFN-Is remains unclear. One hypothesis is that the initial IFN-I response is protective due to the induction of antiviral factors, whereas chronic stimulation promotes inflammation through other ISGs with sustained, elevated expression. Distinct IFN-Is present in the acute versus chronic stages of persistent viral infection may induce divergent cellular immune responses. Tissue compartmentalization may also play a role. The gut is a critical site not only for early HIV-1 infection, but also in driving chronic immune activation [34] . Epithelial barrier dysfunction, partly due to the loss of Th17 cells, leads to the translocation of enteric bacteria from the gut lumen to the lamina propria and systemic circulation, resulting in chronic immune activation [35] [36] [37] . We recently reported increased IFNβ gene expression in the gut, but not the blood, in persons with HIV-1 (PWH) infection compared to age/gender-matched HIV-1 uninfected controls [38] . By contrast, IFNα subtypes were downregulated in PWH, and IFNλ, a type III IFN linked to mucosal immunity in mouse models [39] , was undetectable in these samples [38] . These findings suggest that among the diverse IFN-Is, IFNβ may play a dominant role in the gut during chronic HIV-1 infection. Here, we utilized transcriptomic approaches to evaluate whether the IFNα subtypes and IFNβ exhibit qualitatively different effects on gene expression. We then tracked how interferon-regulated genes were altered during chronic HIV-1 infection in the gut. Our analyses highlight potential mechanisms driven by IFNβ that may influence mucosal HIV-1 pathogenesis. The relative anti-HIV-1 activity of IFNβ in primary LPMCs remains unclear, though studies using PBMCs suggest that IFNβ is relatively potent [40] . We previously reported a spectrum of antiviral potencies of the 12 IFNα subtypes against HIV-1 in primary LPMCs [12] . These data were extended to show a 10-fold difference in 50% inhibitory concentrations (IC50) between IFNα14 and IFNα2 [13] . Using the same 6 LPMC donors used to calculate the IC50s of IFNα14 and IFNα2, we titrated the anti-HIV-1 potencies of IFNα1 (weak), IFNα8 (potent) and IFNβ. The IFN-Is were added into LPMC cultures at the time of infection with HIV-1 BaL . At 4 d post-infection, the percentage of HIV-1 Gag p24+ cells were evaluated by flow cytometry. Sigmoidal curves were fitted into the average inhibition data for 6 donors, and used to calculate IC50 values (pg/ml protein concentration). We also calculated a metric known as 'Vres', which corresponds to the level of residual virus replication at maximal doses of IFN-Is [41] . For comparison, previously reported inhibition curves for IFNα14 and IFNα2 are also shown [13] . IFNα8 showed IC50 concentrations over 10-fold lower than that of IFNα1 (Fig 1) . Notably, IFNβ had a similar potency as IFNα8 and IFNα14. We also calculated IC50s for individual donors (S1A Fig) and show significantly lower inhibition by IFNα2 compared to IFNα8 and IFNα14 (S1B Fig). IFNα8 and IFNα14 reduced HIV-1 infection levels to~30% at the maximal doses tested (10 ng/ml), whereas IFNα1, IFNα2 and IFNβ had higher Vres between 38-42%. However, these differences in Vres were not significant (S1C Fig) . These findings validate prior results on the relative anti-HIV-1 activity of these IFNα subtypes and highlight IFNβ as a potent anti-HIV-1 IFN. Quantitative differences were evident, as increasing the dose of weaker IFNα subtypes should enable these cytokines to inhibit HIV-1 just as well as the potent IFNα subtypes. The stronger anti-HIV-1 potencies of IFNα14 and IFNα8 compared to IFNα2 and IFNα1 were associated with higher ISG induction [12] , but it remained unknown how the other IFNα subtypes and IFNβ compare. To test the ISRE signaling activity of IFN-Is, we used a commercially-available iLite assay (see Methods). The iLite cells are human U937 monocytic cell lines transduced with a luciferase reporter downstream of an ISRE from ISG15, a canonical ISG. Serial 10-fold dilutions of the 12 IFNα subtypes and IFNβ were added into the iLite cells and relative light units were measured after 18 h. 50% effective concentrations (EC50) were then calculated from best-fit sigmoidal plots. Fig 2A highlights the 482-fold EC50 difference in ISRE-activity between IFNα14 and IFNα1. The EC50s of the other IFNα subtypes and IFNβ fell in-between IFNα14 and IFNα1 Data were normalized to mock (untreated) as 100% for each donor. Dose-response curves were generated using a one-phase decay equation in GraphPad Prism 5.0 to determine IC50 values. Vres, the percentage of cells that remain infected relative to the mock at the maximum dose tested, corresponded to plateau values from the decay equation. � Note that data on IFNα14 and IFNα2 were previously published [13] . https://doi.org/10.1371/journal.ppat.1008986.g001 Type I interferons in mucosal HIV-1 infection ( Fig 2B) . Notably, the ISRE EC50 of the IFNα subtypes significantly correlated with anti-HIV-1 potency data from our previous study (Fig 2C) [12] . A significant positive correlation was also observed between ISRE-activity and the IC50 values from the 5 IFN-Is tested in Fig 1 that includes IFNβ (R 2 = 0.87, p = 0.02). Importantly, ISRE EC50 values correlated strongly with published IFNAR2 binding affinity values when comparing the IFNα subtypes ( Fig 2D) [16] . However, the inclusion of IFNβ, which was reported to have a higher IFNAR2 binding affinity than multiple IFNα subtypes [42, 43] , abolished the correlation (Fig 2E) . These data demonstrate that ISRE-activity as measured by the iLite assay can be used to evaluate quantitative differences between the IFN-Is, particularly for the IFNα subtypes. Our data in Figs 1 and 2, as well as data from other studies [12] [13] [14] [15] , provide strong evidence for quantitative differences among the IFN-Is. To uncouple quantitative versus qualitative differences, we normalized our IFN-I treatments for ISRE-activity ( Fig 2B) for unbiased transcriptomics. Purified gut CD4+ T cells from 4 different donors were treated with 100 pg/ml IFNα14, and the other IFN-Is were added at higher concentrations that match the ISRE-activity of this IFNα14 dose (e.g., 48.2 ng/ml IFNα1). Purified CD4+ T cells were evaluated instead IFNα subtypes that had potent activity against HIV-1 in a previous study are highlighted in black. IFNβ is highlighted in green. Note that the EC50s are negative log values; thus the higher the bar, the less concentration is needed to achieve an EC50. (C) ISRE-activity versus HIV-1 inhibition. HIV-1 inhibition values were previously reported [12] showing % inhibition of HIV-1 p24+ cells relative to mock in LPMC cultures. ISRE-activity versus IFNAR2 binding affinity (D) without or (E) with IFNβ. IFNAR2 binding affinity data were previously reported using surface plasmon resonance. For panels C to E, linear regression curves were plotted using Prism 5.0 and evaluated using Pearson statistics. https://doi.org/10.1371/journal.ppat.1008986.g002 Type I interferons in mucosal HIV-1 infection of total LPMCs to reduce potential confounders due to cell type heterogeneity in LPMCs when performing RNAseq. CD4+ T cells account for majority of cells in LPMCs (65%) [44] and are the main cell types for the interaction between HIV-1 and antiviral ISGs. Given that only limited numbers of primary LPMCs can be obtained per donor, we selected IFNα1, IFNα2, IFNα5, IFNα8 and IFNα14, as these were highly expressed in HIV-1-exposed primary pDCs in vitro and in PBMCs during chronic HIV-1 infection in vivo [12, 38] . We also selected IFNβ because it is significantly upregulated in the gut during chronic HIV-1 infection On average, we obtained 34.6 million (range: 10.4 to 167 million) sequence reads per sample (S1 Table) . We first evaluated the transcripts per million (TPM) levels of ISG15, from which the ISRE was genetically linked to luciferase in the iLite assay. All IFNα subtypes tested and IFNβ induced ISG15 to similar levels ( Fig 3A, S2B Fig). In fact, the treatment dose used Type I interferons in mucosal HIV-1 infection also induced the canonical ISGs RSAD2 (viperin) and MX1 (Fig 3B) , as well as OASL, BST2 and APOBEC3G (S3 Table) to similar extents. These data confirmed that the treatments were indeed normalized for ISRE-activity and IFNAR signaling strength. Overall, the 5 IFNα subtypes and IFNβ altered 1,969 genes. Upregulated genes (ISGs) were more prevalent (68-80% of IRGs) than downregulated genes across all IFN-Is ( Fig 3C) . We next compared our IRG set with IRGs catalogued in the INTERFEROME database [5] . A majority (70.8%) of the observed IRGs were found in the INTERFEROME database ( Fig 3D) . We then partitioned the data into individual IFN-Is. Strikingly, there were, on average, 2.7-fold more IFNβ-regulated genes than those regulated by any of the individual IFNα subtypes tested (Fig 3C) . On average, IFNβ upregulated 2.4-fold and downregulated 3.9-fold more genes than the individual IFNα subtypes (Fig 3C) . There was a strong correlation between IFNAR2 binding affinity and the number of IRGs or ISGs (R 2 >0.94, p<0.01), but these correlations were lost if IFNβ was removed (p<0.05). In addition, our current analysis revealed 578 novel IRGs (S2 Table) . Many of these novel IRGs may not encode protein products and/or have tentative gene designations, potentially explaining why these genes are not in the INTERFEROME database. However, some long non-coding RNAs such as NRIR (negative regulator of the interferon response) [47] and BISPR (BST2 interferon stimulated positive regulator) [48] could have critical roles for modulating IFN-I responses. Some repressed protein-coding genes such as CCR9 appeared specific to just one IFNα subtype (S2 Table) . The IFNα subtypes are homologous genes that activate IFNAR, raising questions on whether their differential effects were primarily quantitative. We postulated that if the differences between the IFNα subtypes are mainly quantitative, then we should observe a substantial overlap between the IRGs of cells treated with different IFNα subtypes that were normalized for ISRE-activity. The number of IRGs that overlapped between any two of the 5 tested IFNα subtypes ranged from 59% (IFNα2 vs IFNα5) to 82% (IFNα2 vs IFNα1) ( Fig 4A) . This included a core set of 266 IRGs altered by all 5 IFNα subtypes tested (Fig 4B; S3 Table) . Interestingly, many additional genes appeared to be specific to an IFNα subtype, particularly for IFNα5 and IFNα14 ( Fig 4B and S4 Table) . To test if the IRGs unique to each IFNα subtype could have been regulated by other IFNα subtypes but excluded due to a stringent FDR cut-off of 20%, we investigated the median FDR of these unique genes against the other 4 IFNα subtypes. This analysis is illustrated in S3 Fig, where the FDR values of each of the 201 IFNα5-specific genes or 257 IFNα14-specific genes ( Fig 4B) were plotted against the gene induction datasets for the other IFNα subtypes tested. As shown, majority of the IFNα5 or IFNα14-specific genes had FDR values that were >90% in the other IFNα subtypes. These analyses were expanded on S5 Table, where IFNα-specific IRGs had a median FDR of at least 75%, with most >90%, when tested against genes sets from other IFNα subtypes. Thus, IRGs that were differently expressed by a specific IFNα subtype were unlikely to be significantly altered by the other IFNα subtypes. We next evaluated trends as to whether IRGs altered by at least one IFNα subtype (a total of 1,257 genes) were similarly upregulated or downregulated. These comparisons were based on mean fold-induction values that can be visualized in the heatmap ( Fig 4C) . Surprisingly, a large number of genes (n = 367) that trended to be upregulated by IFNα1, IFNα2, IFNα8 and IFNα14 appeared to be downregulated by IFNα5 (Fig 4C, S6 Table) . As the IRGs were based on an arbitrary cut-off (�1.5× relative to mock, FDR �20%), we next evaluated whether increasing our FC criteria changed the differential expression patterns. At 2×, 2.5× and 3× cut-offs, we still observed genes that were differentially regulated by IFNα5 To determine whether IFNα subtypes induced molecular programs distinct from each other, we subjected the IRGs to Ingenuity Pathway Analyses (IPA) [49] . S7 Table provides a ranked list of activated and inhibited pathways for each IFN-I. As expected, 'Interferon signaling' and 'Pattern Recognition Receptors' were the top induced pathways predicted for the 5 79.0% of IFNα2-regulated genes were found among IFNα1-RGs, whereas 59.7% of IFNα1-RGs were found among IFNα2-RGs. (B) Euler diagram showing the interferome overlap between the 5 IFNα subtypes tested. A core set of 266 IRGs altered by all 5 IFNα subtypes were detected. IFNα-subtype specific genes were highlighted (e.g., 243 for IFNα14, 201 for IFNα5). The total number of IRGs (n = 1,257) include genes not shown that were shared between 2 to 4 IFNα subtypes. (C) Heatmap of IRGs from distinct IFNα subtypes. Highlighted areas in red correspond to core ISGs, whereas those in blue correspond to genes differentially regulated by IFNα5 relative to the four other IFNα subtypes tested. (D) Ingenuity Pathway Analyses of IFNα subtypes, highlighting Z-scores for shared pathways and those predicted to be specific to IFNα5 and IFNα14. https://doi.org/10.1371/journal.ppat.1008986.g004 IFNα subtypes tested ( Fig 4D) . Death Receptor, NFκB and Inflammasome signaling were also highly induced by all IFNα subtypes (S7 Table) . Interestingly, IPA also predicted distinct pathways induced by the IFNα subtypes. CD28 signaling, CTL and NK function, p38 MAPK signaling and sumoylation were predicted for IFNα14 but not the other IFNα subtypes (S7 Table and Fig 4D) . Only the IFNα5 interferome was associated with downregulated apoptosis, PPAR and LXR/RXR activation, likely due to some downregulated genes such as CASP9 (S6 Table) . These differential predictions provide confirmatory evidence of qualitative biological differences in endpoint effector mechanisms induced by different IFNα subtypes. Our data in Fig 3C revealed~970 more IRGs for IFNβ than the individual IFNα subtypes. We pooled IFNα regulated genes independently of the subtype and compared them to IFNβ-regulated genes. Almost half (46.4%) of IFNβ interferome genes were not regulated by any of the 5 IFNα subtypes (Fig 5A) . These included cytokines and cytokine receptor genes such as IL2, IFNGR1, IL21R and TGFB1 ( Fig 5A; S8 Table) . We compared the IPA results for the IFNα subtypes versus IFNβ regulated genes. IFNβ induced more pathways (Fig 5B) than the IFNα subtypes, such as 'Th2 pathway', 'ERK/MAPK signaling' and 'Regulation of actin motility'. These findings indicated that IFNβ induced a broader interferome than IFNα subtypes. Moreover, compared to IFNα, IFNβ likely regulated more cellular pathways in primary gut CD4+ T cells. We recently reported that during chronic, untreated HIV-1 infection, IFN-I inducible antiretroviral genes APOBEC3G, BST2 and MX2, as well as IFNβ, but not IFNα, were expressed to significantly higher levels when compared to HIV-1 uninfected individuals [38] . To expand on these findings, we performed RNAseq on these gut biopsies to more broadly investigate the Table 1 provides the demographic characteristics of this clinical cohort, which included 19 PWH and 13 HIV-1 uninfected individuals [50] . On average, we processed 41.2 million sequence reads from colon biopsies per subject (Table 1) . Filtered data were normalized using Transcripts Per Million (TPM), Trimmed Mean of M values (TMM) [51, 52] and DESeq2 [53] methods. Based on relative log expression plots [54] , the TMM method was most efficient at removing unwanted variation (S6 Fig) . We next determined the expression levels in the colon biopsies in vivo of the 266 'core IRGs' that were similarly regulated by the 5 IFNα subtypes and IFNβ in gut CD4+ T cells in Type I interferons in mucosal HIV-1 infection vitro. The majority (92%; n = 246) of these core IRGs that passed the RNAseq filter criteria were ISGs (e.g., these genes were induced by the IFN-Is tested, not downregulated). Of these 246 core ISGs, 126 (51%) were significantly altered in HIV-1 infected versus uninfected individuals at 5% FDR ( Fig 6A) . The majority (89%) of these altered core ISGs were upregulated during chronic HIV-1 infection. These included the antiretroviral genes APOBEC3G, BST2 and MX2, consistent with our previous report [38] . Genes linked to innate sensing, immunomodulation and cell death/proliferation, as well as negative feedback regulation of the IFN-I response, were also expressed at significantly higher levels in PWH (Fig 6B and 6C , S9 Table) . Since IFNβ induced a broader interferome than all IFNα subtypes tested (Fig 5A) , we evaluated whether ISGs that were unique to IFNβ (IFNβ-specific ISGs) were also induced in the gut during chronic HIV-1 infection. Of the 712 IFNβ-specific IRGs, 57% (n = 406) were ISGs that passed the RNAseq filter criteria (Fig 3C) . Nearly a third of these IFNβ-specific ISGs (28%; n = 112) were significantly altered in PWH compared to HIV-1 uninfected controls (Fig 7A) . Only a few of the IFNβ-specific ISGs were significantly upregulated in PWH (Fig 7A) . By contrast, the vast majority (>90% (n = 102) of the altered IFNβ-specific ISGs were downregulated during chronic HIV-1 infection in the gut (Fig 7A) . These repressed IFNβ-specific ISGs are involved in intracellular vesicle trafficking, transcriptional/translational regulation, protein ubiquitination and transport (Fig 7B and 7C ; S10 Table) . Moreover, several known HIV-1 Type I interferons in mucosal HIV-1 infection cofactors such as TSG101, NUP54, and CUL5 were downregulated [55] [56] [57] . We again emphasize that all these genes were induced by IFNβ in gut CD4 T cells ex vivo (S8 Table) . Given that a great majority of these IFNβ-specific ISGs were downregulated in PWH, we conclude that a significant inversion of IFNβ-specific ISGs was observed during chronic HIV-1 infection in the gut. We next investigated potential mechanisms for how these IFNβ-specific ISGs may have been downregulated in the gut in the presence of high IFNβ levels. Two known negative feedback regulators, USP18 [58] and UNC93B1 [59] , were induced by all IFN-Is tested ex vivo (S3 Table) . Gut IFNβ mRNA levels positively correlated with USP18 and UNC93B1 transcripts (S12 Table) . However, none of the IFNβ-specific ISGs altered in PWH correlated with USP18 (S11 and S13 Tables). By contrast, 89% of these IFNβ-specific ISGs negatively correlated with UNC93B1 (S11 and S13 Tables). Thus, one potential mechanism for the inversion IFNβ-specific ISGs in the gut during chronic HIV-1 infection may be the IFNβ-mediated induction of UNC93B1. The clinical study described in Table 1 involved cohorts where paired blood (PBMCs/plasma) and colon pinch biopsies (up to 30 per donor) were obtained. Colon pinch biopsies (~20) were Type I interferons in mucosal HIV-1 infection pooled for same-day flow-based immunophenotyping [50, 60] and the rest were frozen for later histology and transcriptomics. The study obtained comprehensive data including gut and PBMC IFNα and IFNβ transcript levels, plasma and gut viral loads, gut CD4+ T cell percentages (of CD45+ cells), myeloid activation (CD40 MFI in CD1c+ myeloid DCs), blood CD4+ T cell counts, markers of microbial translocation (sCD27, LPS, LTA), inflammation (CRP, IL6), and epithelial barrier dysfunction (iFABP) ( Table 1 and S11 Table) [38, 50, 60-63]. We investigated how individual altered core ISGs (n = 126) and IFNβ-specific ISGs (n = 112) correlated with these clinically relevant parameters using linear regression models, after adjusting the data for age and gender. We used a 5% FDR threshold for these associations. Five clinically relevant parameters-gut IFNβ mRNA, plasma LPS, gut CD4 T cell frequencies, blood CD4 T cell counts and plasma IL6 levels-correlated significantly with the altered core and IFNβ-specific ISGs (Fig 8 and S11 Table) . The expression of core and IFNβ-specific ISGs significantly correlated with transcript levels of IFNβ (>90%) rather than IFNα (<1%) (S11 Table) . Interestingly, the directionality of the correlations was discordant between these 2 gene sets: higher IFNβ levels correlated with higher expression of 82% of core ISGs, whereas higher IFNβ levels were associated with lower expression of 88% of IFNβ-specific ISGs ( Fig 8A) . Both core ISGs and IFNβ-specific ISGs were significantly associated with plasma LPS levels, but again with discordant directionalities (Fig 8B) . Gut CD4 T cell counts (as a percentage of CD45+ cells) were more significantly associated with core-ISGs (78% of genes negatively correlated) than IFNβ-specific ISGs (50% of genes positively correlated) (Fig 8C) . By contrast, blood CD4 T cell counts were more significantly associated with IFNβ-specific ISGs (87% negatively correlated) than core ISGs (Fig 8D) . Notably, the lower expression of a substantial fraction of IFNβ-specific ISGs (58%) was associated with higher levels of plasma IL6. None of the core ISGs correlated significantly with plasma IL6 at the 5% FDR cut-off. The complete list of genes that correlated with the 5 clinically relevant parameters (gut IFNβ mRNA, plasma LPS, gut CD4 T cell frequencies, blood CD4 T cell count and plasma IL6 levels) are described in S12 Table. We highlight several core ISGs with the highest correlations in Fig 9A, 68] . NLRC5 and LAG3 also positively correlated with plasma LPS levels and inversely correlated with the percentage of CD4+ T cells in the gut (Fig 9C and 9D ). Since T cell exhaustion in persistent LCMV infection has been linked to the suppressive cytokine IL10 [26] , we evaluated if IFNβ mRNA levels correlated with cytokine transcripts in the RNAseq dataset. Notably, IFNβ mRNA levels were associated with increased levels of IL10 and IL10RA, which were in turn correlated with LAG3 ( S7A Fig). IFNβ mRNA levels also correlated with transcript levels of TNFα and IFNγ, but not IL18 and TGFβ (S7B Fig) . These data suggest that increased IFNβ in the gut of chronic PWH may drive genes associated with sustained ISG expression, antigen processing, T cell activation, inflammation and immune exhaustion. Among the IFNβ-specific ISGs (Fig 9; panels in the right half), higher IFNβ transcript levels negatively correlated with: (1) EIF4H, a eukaryotic translational initiation factor (Fig 9E) [69]; (2) SMAD4, a key regulator of TGFβ [70] , which in turn promotes mucosal barrier integrity ( Fig 9F) ; (3) VIMP and SEP15, selenoproteins that regulate protein folding in the endoplasmic reticulum [71, 72] that were linked to anti-inflammatory processes [72] ; and (4) two co-factors of HIV-1 Vpr, NUP54 and MUS81 [56, 73] . NUP54 is a nuclear pore component and MUS81 is an endonuclease; both are involved in maintaining genomic DNA integrity [74, 75] . Reduced expression of VIMP and SEP15 were associated with lower gut CD4 T cell Type I interferons in mucosal HIV-1 infection percentages (Figs 8 and 9G) . Downregulated NUP54 and MUS81 were associated with higher plasma IL6 levels (Fig 9H and 9I) , lower gut CD4 T cell percentages (S12 Table) and blood CD4 T cell counts (Fig 9H and 9I) . Thus, our analyses link elevated IFNβ levels in the gut of PWH to decreased protein translation and decreased protection against DNA damage, protein misfolding and barrier dysfunction. Fig 6A) and IFNβ-specific ISGs (Fig 7A) in gut biopsies from the clinical cohort were determined against (A) gut IFNβ transcripts, (B) plasma LPS levels, (C) gut CD4+ T cell percentages, (D) Blood CD4 T cell counts and (E) Plasma IL6 levels using linear regression models, controlling for age and gender. The clinical cohort included PWH (n = 19) and matched HIV uninfected controls (n = 13). Correlations with FDR � 5% were considered significant; the proportion of those core and beta ISGs are plotted as pie charts, with red, blue and gray depicting positive, negative and no significant correlations, respectively. The top genes in the relevant categories with �50% representation are highlighted; a full list is available in S12 Table. https://doi.org/10.1371/journal.ppat.1008986.g008 Two aspects of type I IFN biology that could influence its translational potential remain insufficiently addressed. First, there is an ongoing debate as to whether the biological effects of IFN-Is are primarily due to quantitative differences in IFNAR signaling capacity. For example, would administration of higher doses of weakly antiviral IFN-Is (e.g., IFNα2 for HIV-1 infection) achieve the same in vivo effect as that of more potent IFN-Is (e.g., IFNα14)? Second, the mechanisms governing how and why IFN-Is become pathogenic during chronic HIV-1 infection remains unclear. Which ISGs are responsible for the discordant effects of IFN-Is at distinct phases of infection with persistent viruses? We have undertaken an unbiased transcriptomics approach to gain deeper insights on these questions. Our group and others reported diverse antiviral potencies of the IFNα subtypes and IFNβ that correlated with their IFNAR binding properties. These data emphasize the contribution of quantitative differences in IFNAR signaling in controlling acute viral infection [12] [13] [14] [15] 76] . However, data from multiple in vivo studies also revealed that the IFNα subtypes may have Expression levels were based on TMM-transformed counts. Linear regression was performed in R statistical package, adjusting for age and gender. A full list of the proportion of core and IFNβ-specific genes that correlated with clinical parameters is presented in S11 Table. https://doi.org/10.1371/journal.ppat.1008986.g009 Type I interferons in mucosal HIV-1 infection qualitative differences in mediating antiviral immunity (reviewed in [2] ). In fact, IFNβ has a significantly higher binding affinity to IFNAR2 than all IFNα subtypes [42, 43] but did not exhibit higher ISRE activity and inhibitory activity against HIV-1. Recently, Schaepler et al utilized saturating concentrations of various IFNα subtypes to inhibit HIV-1 infection in activated PBMCs in vitro. Since 25 canonical ISGs were induced to similar levels at saturating IFN-I doses, the authors concluded that there were no qualitative differences between the IFNα subtypes [15] . We argue that this conclusion is premature, as there are hundreds of ISGs encompassing the interferome [5, 20] . We postulate that if there were no qualitative differences, the interferomes of diverse IFN-Is should have substantial overlap following stimulation of cells with IFN-Is normalized for IFNAR signaling strength. We utilized a luciferase reporter cell line linked to the ISRE of a canonical ISG, ISG15, to normalize for IFNAR signaling strength. The 6 normalized IFN-Is tested (IFNα1, 2, 5, 8, 14 and IFNβ) induced ISG15 and other antiviral ISGs to similar extents in gut CD4+ T cells as expected. Interestingly, despite normalizing for quantitative differences in IFNAR signaling strength, the overlap between the IFNα subtype 'interferomes' were not complete, ranging from 59 to 82%. IFNα14 altered >200 genes not found in any of the other IFNα subtypes. IFNα5 altered >200 additional genes and weakly downregulated genes that were induced by other IFNα subtypes, raising the intriguing possibility that IFNα5 may modulate the overall IFN-I response. While it was possible that these IFNα-subtype specific genes were an artifact of the low numbers of donors used in this study, these genes were not close to statistical significance of being differentially expressed by the other IFNα subtypes, as majority had FDR values >90%. The sample size we used for this work was also counterbalanced by utilizing a homogenous cell subpopulation (purified CD4+ T cells) treated in a controlled fashion in vitro that should decrease overall variability. In fact, >240 canonical ISGs were captured by all IFNs tested including our positive control, ISG15. Interestingly, IFNβ altered nearly three times the number of genes compared to the individual IFNα subtypes we tested. We speculate that the higher binding affinity of IFNβ to the IFNAR2 may contribute in part to the increased gene induction numbers, but it was notable that enhanced binding affinity did not track with ISRE activity. The broader interferome associated with IFNβ was also reported by other groups using PBMCs [77, 78] , although it was unclear whether the doses were normalized for IFNAR signaling strength. Altogether, the incomplete overlap between the interferomes strongly suggests that there are qualitative differences between diverse IFN-Is. The underlying molecular mechanisms for differential interferome induction remain unclear. One possibility is that subtle differences in IFNAR binding may have triggered differential phosphorylation of diverse STATs, JAKs and MAPKs. This possibility is currently under investigation. We speculate that differences in interferome regulation may have consequences for the therapeutic use of IFN-Is in vivo. Clinical administration of IFNα2 and IFNβ in vivo were associated with a multitude of side-effects [79] . One approach to potentially reduce toxicity issues is to utilize IFNα subtypes with a more 'restricted' gene regulation signature. IFNα8 is ten times more potent at inhibiting HIV-1 than IFNα2, but did not induce as many genes as IFNα14. Thus, IFNα8 may be a potent anti-HIV-1 therapeutic with limited side-effects. However, IFNα8 did not seem to inhibit HIV-1 when administered in humanized mice as encoded plasmids via hydrodynamic injection [14] . IFN-Is with desirable immunomodulatory properties may also be useful in HIV-1 curative strategies [13] , as reactivation of latent HIV-1 was insufficient to reduce the reservoir during antiretroviral therapy [80] . IFN-Is could be potent additive drugs for HIV-1 cure by activating immune cells with latent HIV-1 while stimulating immune responses that can kill infected cells and prevent subsequent infection through intrinsic restriction. IFNα14 strongly induced TRAIL+ NK cells and APOBEC3G-mediated hypermutation compared to IFNα2 in humanized mice [13] and was predicted to induce pathways associated with immune function in gut CD4+ T cells compared to IFNα1, 2, 5 and 8. Thus, IFNα14 could be a viable candidate to pursue for HIV-1 curative strategies. The current data could serve as a useful template to design transcriptome-wide studies that extend to the 7 other IFNα subtypes not studied here, as well as other cell types (DCs, NK cells, CD8+ T cells, B cells) in various tissue compartments. Such follow-up studies may aid in designing focused IFN-I biologicals for various clinical applications. Our recent study suggested that IFNβ may play an important role during chronic HIV-1 immunopathogenesis in the gut [38] . We observed downregulated IFNα, but elevated IFNβ levels in colon biopsies from PWH, while IFNβ was rarely detected in the PBMCs and plasma of these patients [38] . Since IFNβ induced a broader interferome than the individual IFNα subtypes, we determined how ISGs induced by all IFN-Is tested ('core ISGs') versus ISGs specifically induced by IFNβ ('IFNβ-specific ISGs') were expressed in the gut biopsies following RNAseq. One limitation of our approach is that colon biopsies encompass other cell types that the interferomes based on gut CD4+ T cells may not capture. Specifically, it is possible that the decreased expression of IFNβ-specific ISGs in PWH relative to uninfected controls may be due to the significant loss CD4+ T cells in PWH. If this was the case, we should have also observed a decrease in core ISG expression in PWH. However, over a hundred core ISGs were upregulated in PWH, correlating significantly with gut IFNβ expression. Alterations in IFNAR expression in mucosal CD4+ T cells during HIV-1 infection may also contribute to our results, but our recent study have not detected significant changes in IFNAR2 expression in mucosal immune cells in PWH [81] . Expanding the interferome analyses to other mucosal cell types may enable deconvolution of the bulk RNAseq data to specific cell types. Consistent with our previous study [38] , many canonical ISGs that include antiviral genes remained upregulated in chronic HIV-1 infection in the gut. Core ISGs positively correlated with IFNβ rather than IFNα transcripts, suggesting that IFNβ drove these responses in the gut. Interestingly, core ISG expression positively correlated with plasma LPS levels. Previously, we showed that many ISGs were upregulated in gut CD4+ T cells following co-incubation with Prevotella stercorea, a gram-negative microbe present in colon tissue of PWH [82] . These findings strengthen a link between microbial translocation, IFNβ, and elevated ISG signatures in the gut during chronic HIV-1 infection. Interestingly, we did not observe correlations between core ISGs and the monocyte activation marker sCD14 or myeloid dendritic cell activation. Other markers such as sCD163 [83, 84] may need to be investigated in future work. IRF9, STAT1 and STAT2 (the components of ISGF3) remained elevated in PWH, suggesting a mechanism for constitutive ISG expression during chronic infection. Notably, IFNβ expression in the gut correlated with gene markers of immune activation and inflammation (CD38, PSMB9, NLRC5, TNFA, IFNG), and exhaustion (LAG3). IFNβ-mediated induction of these immunomodulatory genes may account for how IFNβ contributes to pathogenesis during chronic infection. Specifically, IFNβ levels in the gut were associated with IL10 and IL10RA expression, which in turn correlated with LAG3 levels, suggesting that IFNβ may drive immune exhaustion through the IL10 pathway. It remains to be determined whether these core ISGs are similarly regulated in the systemic circulation, where IFNα subtypes, and not IFNβ, are more prominent [38] . Further, the rationale for why the gut appears primed to express IFNβ remains unclear. A recent study noted that helix 4 of IFNβ may have direct antimicrobial properties [85] . Efforts are ongoing to investigate links between IFNβ levels, IFNβspecific genes and the altered microbiome in PWH. Could IFNβ have distinct biological effects that are likely not due to other IFN-Is? In persistent LCMV infection of mice, neutralization of IFNβ, and not IFNα, accelerated virus clearance and improved T cell responses [86] . In the present study, we observed that a substantial fraction of IFNβ-specific ISGs, but not core ISGs, correlated with plasma levels of the inflammatory cytokine IL6. A link between IFNβ and IL6 has previously been reported [87] . Surprisingly, the IFNβ-specific ISGs that correlated with IL6 were downregulated during chronic HIV-1 infection in the gut. The magnitude of downregulation correlated with higher IFNβ levels, suggesting negative feedback control. Among these negative feedback control genes, we identified UNC93B1 as a potential driver for the downregulation of IFNβ-specific ISGs, possibly through the regulation of TLR7 signaling via endosomal trafficking pathways [59, 88] . It is thought that constitutive expression of antiviral ISGs may protect from virus infection [89, 90] , but decreased protein translation (EIF4H) in PWH may minimize the contribution of antiviral ISGs that are constitutively expressed. Furthermore, our data suggest a potential link between IFNβ, decreased TGFβ signaling (SMAD4), increased unfolded protein response (VIMP and SEP15) and increased DNA damage (NUP54 and MUS81) in chronic HIV-1 infection. These results raise the possibility that genes downregulated by IFNβ could have important consequences for mucosal HIV-1 pathogenesis. One possibility is that these altered IFNβ-specific genes (such as those that decreased protection from DNA damage) may directly contribute to CD4+ T cell death, in line with previous studies linking IFN-Is and CD4+ T cell depletion [91, 92] . Given the predicted pleiotropic effects of IFNβ, our data also raise concerns in utilizing IFNβ for treating chronic Hepatitis B virus infections that became refractory to IFNα treatment [76, 93, 94] . Notably, IFNβ administered <7 days post-symptom onset may help resolve infection with the novel pandemic coronavirus, SARS-CoV-2 [95] . By contrast, delayed IFNβ treatment in murine coronavirus models exacerbated immune pathology [96, 97] . Based on our studies as well as others, understanding the role of distinct IFNs during the course of infection with diverse pathogenic viruses may yield important therapeutic insights. The mechanisms for the differential regulation of core versus IFNβ-specific ISGs during chronic HIV-1 infection remain to be determined. Studies in cell lines reveal that unphosphorylated STAT1, STAT2 and IRF9 can assemble into an unphosphorylated ISGF3 (U-ISGF3) complex that could sustain the expression of canonical ISGs after a single stimulation with IFNβ [90, 98] . Constitutively expressed ISGs regulated by U-ISGF3 included antiviral genes such as BST2, APOBEC3G, MX2 that remained elevated during chronic HIV-1 infection. By contrast, ISGs specifically regulated by phosphorylated ISGF3 are more sensitive to negative feedback regulation. The ISGF3/U-ISGF3 model would imply that core ISGs are regulated by U-ISGF3, whereas the IFNβ-specific ISGs are regulated by ISGF3. Investigations on the phosphorylation status of STAT1, STAT2 and IRF9 in chronic HIV-1 infection are underway. In conclusion, we demonstrate that diverse IFN-Is trigger non-overlapping interferomes in a single cell type, providing strong evidence for qualitative differences between the IFN-Is. Furthermore, conserved and qualitative differences in interferome induction correlated with clinically relevant markers of immunopathogenesis during chronic HIV-1 infection. Further studies on the differences between the IFN-Is and how these cytokines regulate distinct gene expression profiles in various tissue compartments could inform strategies to harness these biologicals and/or block these responses for HIV-1 control. The in vitro studies utilized disaggregated cells from macroscopically normal jejunum tissue that would otherwise be discarded. These tissues were obtained from patients undergoing elective surgery at the University of Colorado Hospital. The patients signed a release form for the unrestricted use of tissues for research following de-identification to laboratory personnel. The Colorado Multiple Institutional Review Board approved the procedures and have given exempt status. Colon pinch biopsies from PWH and age/gender-matched HIV-uninfected controls were obtained from archived samples from a completed, COMIRB-approved clinical study. This clinical study included 24 PWH and 14 HIV-uninfected controls, but archived colon pinch biopsies were available only for 19 PWH and 13 controls, respectively. Study participants signed an informed consent. Clinical parameters that include blood CD4-T cell counts (cells/ μl), plasma viral load, tissue HIV RNA (per CD4 T cell), Tissue CD4 T cells (% viable CD45 + cells), IL-6 (pg/ml), CRP (μg/ml), iFABP (pg/ml), sCD27 (U/ml), CD14 (ng/ml), LPS (pg/ ml), LTA (optical density), gut IFNα and IFNβ transcripts, were reported previously [38]. IFN-Is (PBL Assay Science, Piscataway NJ) were frozen into small aliquots for single-use. iLite Type I IFN Assay Ready cells, purchased from Svar Life Science AB (Malmõ, Sweden, Cat# BM3049) were seeded into a 96-well flat-bottomed cell culture plate and maintained in complete DMEM (with 10% FBS and 1% Penicillin-streptomycin and L-glutamine). Various doses of recombinant IFN-Is were added to corresponding wells. After 18 h, the cells were lysed and luciferase activities were developed using Bright-Glo Luciferase Assay System (Promega, Madison, WI, Cat# E2610) according to manufacturer's instruction. Luciferase activity was subsequently measured using a Perkin-Elmer Victor X5 plate reader. 50% effective concentrations were computed using dose-response curve in Prism 5.0. HIV-1 BaL (NIH AIDS Reagent Program Cat# 510) was prepared in MOLT4-CCR5 cells and titrated using an HIV-1 p24 ELISA (Advanced Bioscience Laboratories, Rockville, MD). 10 ng p24 of HIV-1 BaL / 10 6 LPMCs (n = 6 donors) was spinoculated in the presence or absence of titrated doses of IFNα subtypes and IFNβ (PBL Assay Science). The cells were harvested at 4 d and analyzed by intracellular p24 flow cytometry as previously described [12, 99] . 50% inhibitory concentrations and Vres were calculated using a one-phase decay equation in Prism 5.0 as previously described [12, 13] . CD4+ T cells were purified from LPMCs (n = 4 donors) by negative selection using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell, Vancouver, BC, Canada, Cat# 17952) and followed by flow sorting to reach a purity greater than 99%. 1x10 6 of these purified CD4+ T cells were treated with mock, 100 pg/ml IFNα14, or an equivalent ISRE-activity of IFNα1, 2, 5, 8 and IFNβ. After 18 h, RNA was extracted using RNeasy Mini Kit (Qiagen, Germany, Cat# 74104). RNAseq libraries were constructed from 500 ng RNA using QuantSeq 3' mRNAseq Library Prep Kit (Lexogen, Austria, Cat# 016.96). The RNAseq library quality was verified using 2100 BioAnalyzer (Agilent, Santa Clara, CA) before loaded into a HiSeq 2500 by the Genomics and Microarray Sequencing Core facility at the University of Colorado Anschutz Medical Campus. The 96-well array contained primers for target genes ISG15, ARHGEF3, LAT and AHNAK (S1 Fig) , a reverse transcription control, a genomic DNA control and a positive PCR control as well as the housekeeping gene GAPDH (PPH00150F). Ninety-six-well Custom RT 2 Profiler PCR Arrays were performed according to the manufacturer's instructions (Qiagen, Valencia, Type I interferons in mucosal HIV-1 infection California, USA) using the Bio-Rad CFX-96 Real-time PCR instrument (Bio-Rad, Hercules, California, USA) and Bio-Rad CFX Manager software (Ver. 3.1). cDNA templates were mixed with ready-to-use RT 2 qPCR Master Mixes and 25 μl of the PCR component mix was aliquoted into each well containing predispensed gene-specific primer sets. Each plate was loaded with cDNA from 6 individual samples. Gene expression was normalized according to the average expression level of GAPDH in the same sample. RNA (500 ng) from the colon pinch biopsies were used to prepare the RNAseq libraries. The RNAseq work flow of library construction, quality control, and the subsequent sequencing were similar to that of IFN-I treated gut CD4+ T cells as aforementioned. RNAseq data were downloaded as FASTQ files and their quality examined and visualized using FastQC (Babraham Institute, https://www.bioinformatics.babraham.ac.uk/projects/ fastqc). The removal of Illumina sequencing adapters and the by-base quality screening was performed with cutadapt (https://cutadapt.readthedocs.io/en/stable). The quality screened FASTQ files were mapped to the current human genome assembly GRCh38.p12 using Hisat2 (Johns Hopkins University, https://ccb.jhu.edu/software/hisat2/index.shtml), taking into account the Ensembl ID, gene symbol and gene length. Raw gene expression counts were extracted using featureCounts from the Subread package (Walter+Eliza Hall Institute of Medical Research, http://subread.sourceforge.net). RNAseq raw counts were obtained for gut CD4 T cells treated with 6 IFN-Is (IFNα1, 2, 5, 8, 14 and IFNβ) and mock from 4 donors. Gene counts were normalized using transcripts per million (TPM). Principal component analyses necessitated removal of one donor as the transcriptome of the mock condition was extremely skewed relative to the other 3 donors (S1A Fig). Using edgeR differential expression analysis, IFN regulated genes (IRGs) were defined based on a 1.5-fold change (FC) cutoff relative to mock and a false-discovery rate (FDR) �20%. The RNAseq raw counts data had gene-level read counts for 13 HIV-1-uninfected donors and 19 PWH. Lowly expressed genes which have average read counts less than 5 per library were filtered out. As a result, 19890 out of 43297 genes were kept for further analysis and gene counts were normalized using the trimmed mean of M values (TMM) normalization method from edgeR (version 3.24.3) in R (version 3.5.1) with the "estimateDisp" function and its default settings. The TMM method calculates the scaling normalization factor for each sample by accounting for library size and observed counts, while under the assumption that the majority of genes are not DE. In our case, the TMM method provided the best normalization results in terms of removing the unwanted variation, according to the relative log expression (RLE) plots (S6 Fig) . Two-group differential expression (DE) analysis was performed to test for differences between healthy controls and PWH, using normalized counts in edgeR with default settings and false discovery rate (FDR) of 5% to control for multiple testing. The DE analysis was done in edgeR by the exact test using the "exactTest" function and its default settings for two-group design. The Benjamini-Hochberg procedure (BH step-up procedure) controlling the FDR was used as the multiple testing correction method in this study. The normalized RNAseq counts were further transformed by the variance stabilization and bias reduction method called "regularized log transformation" (rlog) from DESeq2 (version 1.22.2) [53] . The transformed counts were then used as the outcome in a multivariate linear regression model. Specifically, the linear regression models were fit in a gene by gene fashion to test the correlation between RNA expression levels and immunopathogenic markers of chronic HIV-1 infection, with one marker included in the model at a time, while adjusting for age and gender. Based on the results of DE analysis, significantly altered genes from core-ISGs and IFNβ-specific ISGs were selected to test the associations with clinical parameters through the above model, separately. Several immunopathogenic markers were used in this part, including gut IFNβ transcript levels, gut CD4 T cell percentages, blood CD4 T cell counts, plasma IL6 levels and plasma LPS levels. Genes were considered as significantly associated with the corresponding clinical parameter with an FDR cutoff at 5%. In addition, the difference in proportions of significant genes in each gene list were tested with Chi-squared test in R, as well as the difference in proportions of positive correlations of each gene list. All the plots were generated by ggplot2 (version 3.1.1) package in R (version 3.5.1). Next generation sequencing data were deposited in the Sequence Read Archive PRJNA558974 (interferome dataset) and PRJNA558500 (colon pinch biopsies). These were also deposited in the Gene Expression Omnibus archive with accession numbers GSE156844 (interferome dataset) and GSE156861 (colon pinch biopsies). (Left panels) IFNα5-specific genes (n = 201) were classified based on significant induction/suppression in IFNα5-treated cells relative to mock at a 20% FDR cut-off. However, when these genes were evaluated in transcriptome datasets for IFNα1, IFNα2, IFNα8 and IFNα14, most had FDR values >90%, suggesting that these IFNα5-specific genes were not even close to being statistically-significant in these other IFNα subtypes. (Right panels) Evaluation of IFNα14-specific genes against gene datasets for IFNα1, IFNα2, IFNα8 and IFNα5. Bars correspond to the frequency of genes that fell within the FDR values noted in the x-axis. 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We also thank the patients who agreed to use discarded tissue for research purposes and the clinical study participants.