id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350221-8u6q3wfa	Yang, Sung-Tae	HIV virions sense plasma membrane heterogeneity for cell entry	2017-06-28		.txt	text/plain	7788	413	55	We used giant plasma membrane vesicles (GPMVs) derived from HeLa cells that stably express the CD4 receptor and CCR5 co-receptor (CD4 + /CCR5 + ), investigated the lateral partitioning of both receptors in these membranes, and imaged the preferred regions of HIV binding and fusion at the single-particle level (Fig. 1A) . Recognition of Lo/Ld membrane boundaries by HIV Next, we examined whether and how HIV envelope (Env) particles interact with GPMVs. Murine leukemia viruses (MLVs) pseudotyped with HIV gp120/gp41 and fluorescently labeled with mKO-Gag were incubated with CD4 + /CCR5 + GPMVs. We visualized bound particles by epifluorescence microscopy and found that they migrate along The preparation of large-scale phase-separated GPMVs facilitates the study of the lateral distribution of CD4 and CCR5 and the role of ordered lipid domains in HIV entry.	./cache/cord-350221-8u6q3wfa.txt	./txt/cord-350221-8u6q3wfa.txt