id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346314-o9fjpqaj	Jarboui, Mohamed Ali	Nucleolar Protein Trafficking in Response to HIV-1 Tat: Rewiring the Nucleolus	2012-11-15		.txt	text/plain	10004	521	36	Pathway analysis and network reconstruction revealed that Tat expression specifically resulted in the nucleolar enrichment of proteins collectively participating in ribosomal biogenesis, protein homeostasis, metabolic pathways including glycolytic, pentose phosphate, nucleotides and amino acids biosynthetic pathways, stress response, T-cell signaling pathways and genome integrity. Following the detailed annotation of the quantitative abundance changes in the nucleolar protein composition upon Tat expression, we focussed on the Tat-affected cellular complexes and signalling pathways associated with ribosome biogenesis, spliceosome, molecular chaperones, DNA replication and repair and metabolism and discuss their potential involvement in HIV-1 pathogenesis. In this study, we investigated the quantitative changes in the nucleolar proteome of Jurkat T cells constitutively expressing HIV-1 Tat (86aa) versus their Tat-negative counterpart, using stable isotope labelling with amino acids in cell culture (SILAC) technology, followed by ESI tandem mass spectrometry and implemented the experimental approach described in Figure 1A .	./cache/cord-346314-o9fjpqaj.txt	./txt/cord-346314-o9fjpqaj.txt