id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320116-63yvpuqx	Bancroft, Tara	Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes	2019-10-07		.txt	text/plain	11866	656	55	Immunization with a multimerized version of this anti-idiotype induced the proliferation of transgenic murine B cells expressing the iglb12 heavy chain in vivo, despite the presence of deletion and anergy within this population. Using anti-idiotypes specific for the inferred germline version of b12 (iglb12) as baits for single B cell sorting, we identified a subset of human germline BCRs using V H 1-3 with some heavy chain CDRH3 similarity to iglb12. Identification of iglb12-like BCRs using anti-iglb12 idiotypes We next assessed whether the anti-iglb12 idiotypes could be used to identify B cells expressing iglb12-like BCRs from within the polyclonal human B cell repertoire of HIV-uninfected individuals using single B cell sorting followed by RT-PCR amplification and sequencing of heavy and light chain genes. To our knowledge, this study represents the first time antiidiotypes have been used to identify and analyze naive B cells expressing BCRs with similarities to the inferred germline sequence of a broadly neutralizing HIV-1-specific antibody.	./cache/cord-320116-63yvpuqx.txt	./txt/cord-320116-63yvpuqx.txt