id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314753-xflhxb13	Manso, Carmen F.	Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples	2017-06-23		.txt	text/plain	6333	296	48	The percentage of reads mapping to RNA virus genomes in the rRNA-depleted BBV Panel samples was between 40 and 150-fold higher than in corresponding untreated controls. The depth plots in Fig. 3 again show unbiased and even coverages across both genomes, and the percentages of reads mapping to viral targets was again much higher in the rRNA-depleted sample than in the untreated comparator (61-fold and 85-fold for HCV and HPgV respectively). By depleting host-derived nucleic acids and making modifications to an existing library preparation protocol to account for ultra-low RNA input quantities, we have been able to reconstruct effectively full-length genomes of HCV, HEV and HIV from plasma samples with viral loads of 10 4 IU/ml (copies/ml for HIV) and substantial fractions of complete genomes at 10 3 IU/ml. Additionally, our system was able to recover viral sequences from a panel of diverse RNA viruses diluted in human plasma, with a broad correlation between the genomic coverage and depth metrics and approximate concentration.	./cache/cord-314753-xflhxb13.txt	./txt/cord-314753-xflhxb13.txt