id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-304427-r7jt95ko	Pasquato, A.	Heparin enhances the furin cleavage of HIV-1 gp160 peptides	2007-12-22		.txt	text/plain	3356	176	54	The 18mer, 41mer, and 51mer were synthesized on a semi-automatic synthesizer (Applied Biosystems, Mod. 431A) using a Rink amide MBHA resin Abbreviations: HIV-1, human immunodeficiency virus type 1; GAGs, glycosaminoglycans; CD, circular dichroism; AIDS, acquired immunodeficiency syndrome; RP-HPLC, reverse phase high performance liquid chromatography; PC, proprotein convertase; BTMD, before trans membrane domain; MS, mass spectrometry; AMC, 7-amino-4methyl-coumarin; MCA, 7-amido-4-methylcoumarin; TFE, trifluoroethanol; SDS, sodium dodecyl sulfate; Tris-HCl, Tris-(hydroxymethyl) aminomethane-HCl; DMSO, dimethyl sulfoxide; HF, hydrofluoric acid; cmk, chloromethylketone; PBS, phosphate buffer saline (NovaBiochem, La Jolla, 0.48 mmol/g, 0.25 mmol), Boc chemistry and HBTU/HOBt activation. Similarly, peptides derived from the cleavage site of the human respiratory syncytial virus (RSV) fusion glycoprotein bind heparin and cellular GAGs [26] . Heparin was shown to strongly interact with the 41mer and 51mer peptides, inducing conformational changes, thereby exposing site1 for cleavage.	./cache/cord-304427-r7jt95ko.txt	./txt/cord-304427-r7jt95ko.txt