id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263452-y2ral8nx	Watanabe, Yasunori	Site-specific glycan analysis of the SARS-CoV-2 spike	2020-05-04		.txt	text/plain	2073	121	50	To resolve the site-specific glycosylation of SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo-electron microscopy (cryo-EM) structure, albeit without glycan processing blockade using kifunensine (4). The shielding of receptor binding sites by glycans is a common feature of viral glycoproteins, as observed on SARS-CoV-1 S (10, 13), HIV-1 Env (27) , influenza HA (28, 29) , and LASV GPC (24). For example, one of the most densely glycosylated viral spike proteins is HIV-1 Env, which exhibits ~60% oligomannose-type glycans (21, 34) . This suggests that SARS-CoV-2 S protein is less densely glycosylated and that the glycans form less of a shield compared with other viral glycoproteins including HIV-1 Env and LASV GPC, which may be beneficial for the elicitation of neutralizing antibodies. SARS-CoV-2 spike site-specific N-linked glycan analysis	./cache/cord-263452-y2ral8nx.txt	./txt/cord-263452-y2ral8nx.txt