id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259503-dkfrk71a	Smith, Sarah E.	Sherlock Genomes — viral investigator	2013-02-15		.txt	text/plain	806	54	56	Deep sequencing technologies and stateof-the-art bioinformatics techniques have revolutionized the way that RNA viruses, a notoriously variable group of pathogens, can be identified and characterized. By using de novo assembly of short reads, the authors showed that this method could generate full genomes for viruses within all four major HIV-1 genetic groups. Even when almost nothing is known about the agent of a viral infection, deep sequencing technologies can identify a pathogen and produce the full genome, fast. When SARS-CoV began infecting people in 2002, it took a large team to generate a full genome by capillary sequencing. 2 used random priming to amplify viral RNA isolated from the Saudi Arabian patient, and then deep-sequenced the genome. Knowing the sequence of the whole genome enabled the authors to further characterize this new virus, named human CoV (HCoV)-EMC2012. Universal amplification, next-generation sequencing, and assembly of HIV-1 genomes	./cache/cord-259503-dkfrk71a.txt	./txt/cord-259503-dkfrk71a.txt