id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011457-hqxybv1k	Kirui, James	Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release	2020-05-19		.txt	text/plain	5612	249	46	To enable simple and highly sensitive measurement of virus release from transfected cells, we generated HIV-1 reporter viruses in which Nanoluciferase (NanoLuc) was inserted between the MA and CA domains of Gag (Gag-iNanoLuc). We generated viruses using the pNL4-3 Gag-iNanoLuc vector complemented with different ratios of the WT HIV-1 molecular clone pNL4-3 and tested their infectivity by measuring the HIV-1 Tat-driven firefly luciferase activity in TZM-bl cells. These results demonstrate that the Gag-iNanoLuc vector provides a highly sensitive and quantitative tool for measuring the effects of Gag mutations, host cell restriction factors, and small-molecule inhibitors on HIV-1 particle assembly and release. The Gag-NanoLuc fusion protein is expressed in the cell and released at similar levels to WT Gag, thereby enabling simple yet highly sensitive quantification of viral gene expression and virus particle production by measurement of the NanoLuc reporter protein bioluminescent activity in the cell lysates and supernatants.	./cache/cord-011457-hqxybv1k.txt	./txt/cord-011457-hqxybv1k.txt