id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000868-vnwpzsu8	Eissmann, Kristin	HIV-1 Fusion Is Blocked through Binding of GB Virus C E2D Peptides to the HIV-1 gp41 Disulfide Loop	2013-01-22		.txt	text/plain	9072	425	51	Performing a virus-cell fusion assay and temperature-arrested HIV-infection kinetics, we provide evidence that the HIV-inhibitory E2 peptides interfere with late HIV-1 entry steps after the engagement of gp120 with CD4 receptor and coreceptor. Using synthetic peptides presenting different regions of E2, we recently demonstrated that this interference with HIV-1 entry can be ascribed to the N-terminal part of the GBV-C E2 protein ranging from residue 29 to 72 (according to GenBank accession no. The Effect of GBV-C E2 Peptides Arises After gp120/CD4 and gp120/coreceptor engagement To find out whether early or late steps of HIV-1 entry are affected by the E2 peptides, the E2 peptide activity was determined before and after gp120/CD4 engagement using the Vpr-b-lactamase (Vpr-BlaM) enzyme-based virus-cell fusion assay under standard (pre-CD4 binding) and temperature-arrested state (TAS; post-CD4 binding) conditions, respectively.	./cache/cord-000868-vnwpzsu8.txt	./txt/cord-000868-vnwpzsu8.txt