id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-340220-raqapqg0	Potel, Julie	EWI‐2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry	2013-02-16		.txt	text/plain	11314	623	56	We took advantage of these permissive cells expressing mCD81 and the previously described MT81/MT81w monoclonal antibodies (mAbs) (Silvie et al., 2006) to analyse the role of tetraspanin web-associated CD81 in HCV infection. Interestingly, as shown for one representative cell clone, cells expressing EWI-2wint exhibited a stronger staining with MT81w, indicating that EWI-2wint modifies CD81 membrane organization, probably by increasing the association of CD81 with the tetraspanin web. To strengthen the hypothesis that EWI-2wint induces a change in CD81 organization at the plasma membrane, we next analysed the membrane dynamics and partitioning of human CD81 (hCD81) molecules in Huh-7 cells expressing or lacking EWI-2wint by single-molecule fluorescence microscopy, which is a method of choice to characterize the diffusion of proteins and lipids in different regions of the plasma membrane (Owen et al., 2009 ). D. Huh-7w7/mCD81 clones expressing pcDNA3.1, EWI-2 or EWI-2wint were stained with MT81 and MT81w mAbs followed by PE-labelled secondary antibody and analysed by flow cytometry.	./cache/cord-340220-raqapqg0.txt	./txt/cord-340220-raqapqg0.txt