id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-299719-bvdsz626	Fournier, C.	Are trans‐complementation systems suitable for hepatitis C virus life cycle studies?	2013-02-14		.txt	text/plain	4511	262	47	Next, the development of cell-culture-grown hepatitis C virus (HCVcc) systems that can assemble and release of infectious viral particles has made it possible to the study structural regions using trans-complementation systems. A series of replicon RNAs carrying mutations (in the NS3, NS4B, NS5A and NS5B regions) that abolished replication were transfected into Huh-7 hepatoma cells harbouring autonomous replicating HCV helper RNAs. In this context, only NS5A mutations in the low complexity sequence I (LCS I) domain have been efficiently rescued ( Table 1 ). These results have clarified the role of Core, p7 and LDs in pseudo-infectious particle production and have indicated that some steps of virus assembly take place around LDs. Various trans-packaging systems have been developed with subgenomic replicons. Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteins Naturally occurring hepatitis C virus subgenomic deletion mutants replicate efficiently in Huh-7 cells and are trans-packaged in vitro to generate infectious defective particles	./cache/cord-299719-bvdsz626.txt	./txt/cord-299719-bvdsz626.txt