id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-284904-qw1ig2v4	Mizui, Tomokazu	Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy	2009-09-17		.txt	text/plain	4099	250	49	In this study, we investigated the role of autophagy on hepatitis C virus (HCV) RNA replication and demonstrated anti-HCV effects of an autophagic proteolysis inhibitor, chloroquine. Inhibition of autophagy and replication of HCV replicon Cells were treated with 3-methyladenine (10 mM) or mixture of E64d (1 lg/ml) and pepstatin A (1 lg/ml), chloroquine (10 -7 -10 -3 M), interferon (IFN)a (100 U/ml) for 18 h, the levels of replication of HCV replicon were assessed by luciferase assay. To clarify the role of autophagy on the replication of HCV, Huh7/ Rep-Feo cells were treated with 3-methyladenine (10 mM) or a mixture of E64d (10 lg/ml) and pepstatin A (10 lg/ml) which inhibited autophagic protein degradation. Anti-HCV effect of chloroquine independent of IFN signaling pathway IFN-inducible double-stranded RNA-activated protein kinase R (PKR) plays a key antiviral role against hepatitis C virus [26, 27] .	./cache/cord-284904-qw1ig2v4.txt	./txt/cord-284904-qw1ig2v4.txt