id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-264936-3posyr5n	Mohammadzadeh, Sara	Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications	2014-11-24		.txt	text/plain	6202	292	49	RESULTS: The codon-optimized gene had an increased adaptation index value (from 0.65 to 0.85) and reduced GC content (from 62.62 to 51.05) in tobacco and removed the possible deleterious effect of "GGTAAG" splice site in native HCVcp. Blotting assays via specific antibodies confirmed the expression of the 15 kDa HCVcp. The expression level of HCVcp was enhanced by 4-5 times in P19 co-agroinfiltrated plants with better outcomes for PVX, compared to pBI121 vector (0.022% versus 0.019% of the total soluble protein). In this study, we aimed to: i) Construct an efficient transient tobacco expression system for HCVcp N-121 by designing a highly codon-optimized gene and employment of the Iranian Jafarabadi-tobacco plant cultivar which is a high biomass producer ii) Evaluate the expression level of HCVcp for a potato virus X-based vector (PVX) (32) com-pared to a classic pBI121 binary plant vector in co-agroinfiltration with P19 gene silencing suppressor plasmid and iii) Assess the antigenicity of this tobacco-derived HCVcp for potential clinical (diagnostic and vaccine formulation) applications.	./cache/cord-264936-3posyr5n.txt	./txt/cord-264936-3posyr5n.txt