id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000252-iejfgkst	Chen, YanYi	Calciomics: prediction and analysis of EF-hand calcium binding proteins by protein engineering	2010-02-07		.txt	text/plain	4220	253	51	In this paper we first describe our development of a grafting approach to understand site-specific Ca(2+) binding properties of EF-hand proteins with a helix-loop-helix Ca(2+) binding motif, then summarize our prediction and identification of EF-hand Ca(2+) binding sites on a genome-wide scale in bacteria and virus, and next report the application of the grafting approach to probe the metal binding capability of predicted EF-hand motifs within the streptococcal hemoprotein receptor (Shr) of Streptococcus pyrogenes and the nonstructural protein 1 (nsP1) of Sindbis virus. We then report our results following application of the grafting approach to probe calcium binding capabilities in streptococcal hemoprotein receptor (Shr) of Streptococcus pyrogenes and the nonstructural protein of Sindbis virus. Figure 3 shows our grafting approach in obtaining site-specific calcium binding affinity using domain1 CD2 as a scaffold protein.	./cache/cord-000252-iejfgkst.txt	./txt/cord-000252-iejfgkst.txt