id sid tid token lemma pos cord-351964-hduv0ur4 1 1 key key JJ cord-351964-hduv0ur4 1 2 : : : cord-351964-hduv0ur4 1 3 cord-351964-hduv0ur4 cord-351964-hduv0ur4 JJ cord-351964-hduv0ur4 1 4 authors author NNS cord-351964-hduv0ur4 1 5 : : : cord-351964-hduv0ur4 2 1 nan nan NNP cord-351964-hduv0ur4 2 2 title title NN cord-351964-hduv0ur4 2 3 : : : cord-351964-hduv0ur4 2 4 Sorting sorting NN cord-351964-hduv0ur4 2 5 of of IN cord-351964-hduv0ur4 2 6 progeny progeny JJ cord-351964-hduv0ur4 2 7 coronavirus coronavirus NN cord-351964-hduv0ur4 2 8 from from IN cord-351964-hduv0ur4 2 9 condensed condense VBN cord-351964-hduv0ur4 2 10 secretory secretory JJ cord-351964-hduv0ur4 2 11 proteins protein NNS cord-351964-hduv0ur4 2 12 at at IN cord-351964-hduv0ur4 2 13 the the DT cord-351964-hduv0ur4 2 14 exit exit NN cord-351964-hduv0ur4 2 15 from from IN cord-351964-hduv0ur4 2 16 the the DT cord-351964-hduv0ur4 2 17 trans trans NNP cord-351964-hduv0ur4 2 18 - - NNP cord-351964-hduv0ur4 2 19 Golgi Golgi NNP cord-351964-hduv0ur4 2 20 network network NN cord-351964-hduv0ur4 2 21 of of IN cord-351964-hduv0ur4 2 22 AtT20 AtT20 NNP cord-351964-hduv0ur4 2 23 cells cell NNS cord-351964-hduv0ur4 2 24 date date NN cord-351964-hduv0ur4 2 25 : : : cord-351964-hduv0ur4 2 26 1987 1987 CD cord-351964-hduv0ur4 2 27 - - SYM cord-351964-hduv0ur4 2 28 09 09 CD cord-351964-hduv0ur4 2 29 - - HYPH cord-351964-hduv0ur4 2 30 01 01 CD cord-351964-hduv0ur4 2 31 journal journal NN cord-351964-hduv0ur4 2 32 : : : cord-351964-hduv0ur4 2 33 J J NNP cord-351964-hduv0ur4 2 34 Cell Cell NNP cord-351964-hduv0ur4 2 35 Biol Biol NNP cord-351964-hduv0ur4 2 36 DOI DOI NNP cord-351964-hduv0ur4 2 37 : : : cord-351964-hduv0ur4 3 1 nan nan NNP cord-351964-hduv0ur4 3 2 sha sha NNP cord-351964-hduv0ur4 3 3 : : : cord-351964-hduv0ur4 4 1 f1b5cde5a242e912703bae5311866136ef780c9d f1b5cde5a242e912703bae5311866136ef780c9d NNP cord-351964-hduv0ur4 4 2 doc_id doc_id CD cord-351964-hduv0ur4 4 3 : : : cord-351964-hduv0ur4 4 4 351964 351964 CD cord-351964-hduv0ur4 4 5 cord_uid cord_uid NNS cord-351964-hduv0ur4 4 6 : : : cord-351964-hduv0ur4 4 7 hduv0ur4 hduv0ur4 ADD cord-351964-hduv0ur4 5 1 Murine murine JJ cord-351964-hduv0ur4 5 2 hepatitis hepatitis NN cord-351964-hduv0ur4 5 3 virus virus NN cord-351964-hduv0ur4 5 4 ( ( -LRB- cord-351964-hduv0ur4 5 5 strain strain NN cord-351964-hduv0ur4 5 6 A59 A59 NNP cord-351964-hduv0ur4 5 7 ) ) -RRB- cord-351964-hduv0ur4 6 1 , , , cord-351964-hduv0ur4 6 2 ( ( -LRB- cord-351964-hduv0ur4 6 3 MHV MHV NNP cord-351964-hduv0ur4 6 4 - - HYPH cord-351964-hduv0ur4 6 5 A59 A59 NNP cord-351964-hduv0ur4 6 6 ) ) -RRB- cord-351964-hduv0ur4 6 7 is be VBZ cord-351964-hduv0ur4 6 8 a a DT cord-351964-hduv0ur4 6 9 coronavirus coronavirus NN cord-351964-hduv0ur4 6 10 that that WDT cord-351964-hduv0ur4 6 11 buds bud NNS cord-351964-hduv0ur4 6 12 into into IN cord-351964-hduv0ur4 6 13 pre pre JJ cord-351964-hduv0ur4 6 14 - - JJ cord-351964-hduv0ur4 6 15 Golgi golgi JJ cord-351964-hduv0ur4 6 16 compartments compartment NNS cord-351964-hduv0ur4 6 17 and and CC cord-351964-hduv0ur4 6 18 then then RB cord-351964-hduv0ur4 6 19 exploits exploit VBZ cord-351964-hduv0ur4 6 20 the the DT cord-351964-hduv0ur4 6 21 exocytic exocytic JJ cord-351964-hduv0ur4 6 22 pathway pathway NN cord-351964-hduv0ur4 6 23 of of IN cord-351964-hduv0ur4 6 24 the the DT cord-351964-hduv0ur4 6 25 host host NN cord-351964-hduv0ur4 6 26 cell cell NN cord-351964-hduv0ur4 6 27 to to TO cord-351964-hduv0ur4 6 28 reach reach VB cord-351964-hduv0ur4 6 29 the the DT cord-351964-hduv0ur4 6 30 exterior exterior NN cord-351964-hduv0ur4 6 31 . . . cord-351964-hduv0ur4 7 1 The the DT cord-351964-hduv0ur4 7 2 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 7 3 cells cell NNS cord-351964-hduv0ur4 7 4 in in IN cord-351964-hduv0ur4 7 5 which which WDT cord-351964-hduv0ur4 7 6 replication replication NN cord-351964-hduv0ur4 7 7 of of IN cord-351964-hduv0ur4 7 8 this this DT cord-351964-hduv0ur4 7 9 virus virus NN cord-351964-hduv0ur4 7 10 is be VBZ cord-351964-hduv0ur4 7 11 usually usually RB cord-351964-hduv0ur4 7 12 studied study VBN cord-351964-hduv0ur4 7 13 have have VBP cord-351964-hduv0ur4 7 14 only only RB cord-351964-hduv0ur4 7 15 a a DT cord-351964-hduv0ur4 7 16 constitutive constitutive JJ cord-351964-hduv0ur4 7 17 exocytic exocytic JJ cord-351964-hduv0ur4 7 18 pathway pathway NN cord-351964-hduv0ur4 7 19 that that WDT cord-351964-hduv0ur4 7 20 the the DT cord-351964-hduv0ur4 7 21 virus virus NN cord-351964-hduv0ur4 7 22 uses use VBZ cord-351964-hduv0ur4 7 23 . . . cord-351964-hduv0ur4 8 1 MHV MHV NNP cord-351964-hduv0ur4 8 2 - - HYPH cord-351964-hduv0ur4 8 3 A59 A59 NNP cord-351964-hduv0ur4 8 4 also also RB cord-351964-hduv0ur4 8 5 infects infect VBZ cord-351964-hduv0ur4 8 6 , , , cord-351964-hduv0ur4 8 7 albeit albeit IN cord-351964-hduv0ur4 8 8 inefficiently inefficiently RB cord-351964-hduv0ur4 8 9 , , , cord-351964-hduv0ur4 8 10 AtT20 AtT20 NNP cord-351964-hduv0ur4 8 11 cells cell NNS cord-351964-hduv0ur4 8 12 , , , cord-351964-hduv0ur4 8 13 murine murine JJ cord-351964-hduv0ur4 8 14 pituitary pituitary JJ cord-351964-hduv0ur4 8 15 tumor tumor NN cord-351964-hduv0ur4 8 16 cells cell NNS cord-351964-hduv0ur4 8 17 with with IN cord-351964-hduv0ur4 8 18 a a DT cord-351964-hduv0ur4 8 19 regulated regulate VBN cord-351964-hduv0ur4 8 20 as as RB cord-351964-hduv0ur4 8 21 well well RB cord-351964-hduv0ur4 8 22 as as IN cord-351964-hduv0ur4 8 23 a a DT cord-351964-hduv0ur4 8 24 constitutive constitutive JJ cord-351964-hduv0ur4 8 25 exocytic exocytic JJ cord-351964-hduv0ur4 8 26 pathway pathway NN cord-351964-hduv0ur4 8 27 . . . cord-351964-hduv0ur4 9 1 Here here RB cord-351964-hduv0ur4 9 2 we -PRON- PRP cord-351964-hduv0ur4 9 3 examine examine VBP cord-351964-hduv0ur4 9 4 AtT20 AtT20 NNP cord-351964-hduv0ur4 9 5 cells cell NNS cord-351964-hduv0ur4 9 6 at at IN cord-351964-hduv0ur4 9 7 early early JJ cord-351964-hduv0ur4 9 8 times time NNS cord-351964-hduv0ur4 9 9 after after IN cord-351964-hduv0ur4 9 10 the the DT cord-351964-hduv0ur4 9 11 infection infection NN cord-351964-hduv0ur4 9 12 , , , cord-351964-hduv0ur4 9 13 when when WRB cord-351964-hduv0ur4 9 14 the the DT cord-351964-hduv0ur4 9 15 Golgi Golgi NNP cord-351964-hduv0ur4 9 16 apparatus apparatus NN cord-351964-hduv0ur4 9 17 retains retain VBZ cord-351964-hduv0ur4 9 18 its -PRON- PRP$ cord-351964-hduv0ur4 9 19 morphological morphological JJ cord-351964-hduv0ur4 9 20 and and CC cord-351964-hduv0ur4 9 21 biochemical biochemical JJ cord-351964-hduv0ur4 9 22 integrity integrity NN cord-351964-hduv0ur4 9 23 . . . cord-351964-hduv0ur4 10 1 We -PRON- PRP cord-351964-hduv0ur4 10 2 observe observe VBP cord-351964-hduv0ur4 10 3 that that IN cord-351964-hduv0ur4 10 4 progeny progeny JJ cord-351964-hduv0ur4 10 5 coronavirus coronavirus NN cord-351964-hduv0ur4 10 6 and and CC cord-351964-hduv0ur4 10 7 secretory secretory JJ cord-351964-hduv0ur4 10 8 protein protein NN cord-351964-hduv0ur4 10 9 destined destine VBN cord-351964-hduv0ur4 10 10 for for IN cord-351964-hduv0ur4 10 11 the the DT cord-351964-hduv0ur4 10 12 secretory secretory JJ cord-351964-hduv0ur4 10 13 granules granule NNS cord-351964-hduv0ur4 10 14 of of IN cord-351964-hduv0ur4 10 15 the the DT cord-351964-hduv0ur4 10 16 regulated regulated JJ cord-351964-hduv0ur4 10 17 exocytic exocytic JJ cord-351964-hduv0ur4 10 18 pathway pathway NN cord-351964-hduv0ur4 10 19 traverse traverse NN cord-351964-hduv0ur4 10 20 the the DT cord-351964-hduv0ur4 10 21 same same JJ cord-351964-hduv0ur4 10 22 Golgi Golgi NNP cord-351964-hduv0ur4 10 23 stacks stack NNS cord-351964-hduv0ur4 10 24 and and CC cord-351964-hduv0ur4 10 25 accumulate accumulate VBP cord-351964-hduv0ur4 10 26 in in IN cord-351964-hduv0ur4 10 27 the the DT cord-351964-hduv0ur4 10 28 trans trans NNP cord-351964-hduv0ur4 10 29 - - NNP cord-351964-hduv0ur4 10 30 Golgi Golgi NNP cord-351964-hduv0ur4 10 31 network network NN cord-351964-hduv0ur4 10 32 . . . cord-351964-hduv0ur4 11 1 Their -PRON- PRP$ cord-351964-hduv0ur4 11 2 pathways pathway NNS cord-351964-hduv0ur4 11 3 diverge diverge VBP cord-351964-hduv0ur4 11 4 at at IN cord-351964-hduv0ur4 11 5 this this DT cord-351964-hduv0ur4 11 6 site site NN cord-351964-hduv0ur4 11 7 , , , cord-351964-hduv0ur4 11 8 the the DT cord-351964-hduv0ur4 11 9 condensed condense VBN cord-351964-hduv0ur4 11 10 secretory secretory JJ cord-351964-hduv0ur4 11 11 proteins protein NNS cord-351964-hduv0ur4 11 12 including include VBG cord-351964-hduv0ur4 11 13 the the DT cord-351964-hduv0ur4 11 14 ACTH ACTH NNP cord-351964-hduv0ur4 11 15 going go VBG cord-351964-hduv0ur4 11 16 to to IN cord-351964-hduv0ur4 11 17 the the DT cord-351964-hduv0ur4 11 18 secretory secretory JJ cord-351964-hduv0ur4 11 19 granules granule NNS cord-351964-hduv0ur4 11 20 and and CC cord-351964-hduv0ur4 11 21 the the DT cord-351964-hduv0ur4 11 22 coronavirus coronavirus NN cord-351964-hduv0ur4 11 23 to to TO cord-351964-hduv0ur4 11 24 post post VB cord-351964-hduv0ur4 11 25 - - JJ cord-351964-hduv0ur4 11 26 Golgi golgi JJ cord-351964-hduv0ur4 11 27 transport transport NN cord-351964-hduv0ur4 11 28 vesicles vesicle NNS cord-351964-hduv0ur4 11 29 devoid devoid JJ cord-351964-hduv0ur4 11 30 of of IN cord-351964-hduv0ur4 11 31 ACTH ACTH NNP cord-351964-hduv0ur4 11 32 . . . cord-351964-hduv0ur4 12 1 On on IN cord-351964-hduv0ur4 12 2 very very RB cord-351964-hduv0ur4 12 3 rare rare JJ cord-351964-hduv0ur4 12 4 occasions occasion NNS cord-351964-hduv0ur4 12 5 there there EX cord-351964-hduv0ur4 12 6 is be VBZ cord-351964-hduv0ur4 12 7 missorting missorte VBG cord-351964-hduv0ur4 12 8 such such JJ cord-351964-hduv0ur4 12 9 that that IN cord-351964-hduv0ur4 12 10 aggregates aggregate NNS cord-351964-hduv0ur4 12 11 of of IN cord-351964-hduv0ur4 12 12 condensed condense VBN cord-351964-hduv0ur4 12 13 secretory secretory JJ cord-351964-hduv0ur4 12 14 proteins protein NNS cord-351964-hduv0ur4 12 15 and and CC cord-351964-hduv0ur4 12 16 viruses virus NNS cord-351964-hduv0ur4 12 17 occur occur VBP cord-351964-hduv0ur4 12 18 together together RB cord-351964-hduv0ur4 12 19 in in IN cord-351964-hduv0ur4 12 20 post post JJ cord-351964-hduv0ur4 12 21 - - JJ cord-351964-hduv0ur4 12 22 Golgi golgi JJ cord-351964-hduv0ur4 12 23 vesicles vesicle NNS cord-351964-hduv0ur4 12 24 . . . cord-351964-hduv0ur4 13 1 We -PRON- PRP cord-351964-hduv0ur4 13 2 conclude conclude VBP cord-351964-hduv0ur4 13 3 that that IN cord-351964-hduv0ur4 13 4 the the DT cord-351964-hduv0ur4 13 5 constitutive constitutive JJ cord-351964-hduv0ur4 13 6 and and CC cord-351964-hduv0ur4 13 7 regulated regulated JJ cord-351964-hduv0ur4 13 8 exocytic exocytic JJ cord-351964-hduv0ur4 13 9 pathways pathway NNS cord-351964-hduv0ur4 13 10 , , , cord-351964-hduv0ur4 13 11 identified identify VBN cord-351964-hduv0ur4 13 12 respectively respectively RB cord-351964-hduv0ur4 13 13 by by IN cord-351964-hduv0ur4 13 14 the the DT cord-351964-hduv0ur4 13 15 progeny progeny JJ cord-351964-hduv0ur4 13 16 virions virion NNS cord-351964-hduv0ur4 13 17 and and CC cord-351964-hduv0ur4 13 18 the the DT cord-351964-hduv0ur4 13 19 secretory secretory JJ cord-351964-hduv0ur4 13 20 protein protein NN cord-351964-hduv0ur4 13 21 ACTH ACTH NNP cord-351964-hduv0ur4 13 22 , , , cord-351964-hduv0ur4 13 23 diverge diverge VBP cord-351964-hduv0ur4 13 24 at at IN cord-351964-hduv0ur4 13 25 the the DT cord-351964-hduv0ur4 13 26 exit exit NN cord-351964-hduv0ur4 13 27 from from IN cord-351964-hduv0ur4 13 28 the the DT cord-351964-hduv0ur4 13 29 trans trans NNP cord-351964-hduv0ur4 13 30 - - NNP cord-351964-hduv0ur4 13 31 Golgi Golgi NNP cord-351964-hduv0ur4 13 32 network network NN cord-351964-hduv0ur4 13 33 . . . cord-351964-hduv0ur4 14 1 I -PRON- PRP cord-351964-hduv0ur4 14 2 N n CD cord-351964-hduv0ur4 14 3 cells cell NNS cord-351964-hduv0ur4 14 4 of of IN cord-351964-hduv0ur4 14 5 the the DT cord-351964-hduv0ur4 14 6 anterior anterior JJ cord-351964-hduv0ur4 14 7 pituitary pituitary JJ cord-351964-hduv0ur4 14 8 tumor tumor NN cord-351964-hduv0ur4 14 9 cell cell NN cord-351964-hduv0ur4 14 10 line line NN cord-351964-hduv0ur4 14 11 ART20 art20 VBP cord-351964-hduv0ur4 14 12 there there EX cord-351964-hduv0ur4 14 13 are be VBP cord-351964-hduv0ur4 14 14 two two CD cord-351964-hduv0ur4 14 15 exocytic exocytic JJ cord-351964-hduv0ur4 14 16 pathways pathway NNS cord-351964-hduv0ur4 15 1 , , , cord-351964-hduv0ur4 15 2 one one CD cord-351964-hduv0ur4 15 3 is be VBZ cord-351964-hduv0ur4 15 4 constitutive constitutive JJ cord-351964-hduv0ur4 15 5 and and CC cord-351964-hduv0ur4 15 6 the the DT cord-351964-hduv0ur4 15 7 other other JJ cord-351964-hduv0ur4 15 8 is be VBZ cord-351964-hduv0ur4 15 9 regulated regulate VBN cord-351964-hduv0ur4 15 10 , , , cord-351964-hduv0ur4 15 11 such such JJ cord-351964-hduv0ur4 15 12 that that IN cord-351964-hduv0ur4 15 13 secretion secretion NN cord-351964-hduv0ur4 15 14 is be VBZ cord-351964-hduv0ur4 15 15 enhanced enhance VBN cord-351964-hduv0ur4 15 16 by by IN cord-351964-hduv0ur4 15 17 seeretagogues seeretagogue NNS cord-351964-hduv0ur4 15 18 ( ( -LRB- cord-351964-hduv0ur4 15 19 Gumbiner Gumbiner NNP cord-351964-hduv0ur4 15 20 and and CC cord-351964-hduv0ur4 15 21 Kelly Kelly NNP cord-351964-hduv0ur4 15 22 , , , cord-351964-hduv0ur4 15 23 1982 1982 CD cord-351964-hduv0ur4 15 24 ) ) -RRB- cord-351964-hduv0ur4 15 25 . . . cord-351964-hduv0ur4 16 1 The the DT cord-351964-hduv0ur4 16 2 major major JJ cord-351964-hduv0ur4 16 3 envelope envelope NN cord-351964-hduv0ur4 16 4 glycoprotein glycoprotein NN cord-351964-hduv0ur4 16 5 of of IN cord-351964-hduv0ur4 16 6 an an DT cord-351964-hduv0ur4 16 7 endogenous endogenous JJ cord-351964-hduv0ur4 16 8 murine murine JJ cord-351964-hduv0ur4 16 9 leukemia leukemia NN cord-351964-hduv0ur4 16 10 virus virus NN cord-351964-hduv0ur4 16 11 ( ( -LRB- cord-351964-hduv0ur4 16 12 Gumbiner Gumbiner NNP cord-351964-hduv0ur4 16 13 and and CC cord-351964-hduv0ur4 16 14 Kelly Kelly NNP cord-351964-hduv0ur4 16 15 , , , cord-351964-hduv0ur4 16 16 1982 1982 CD cord-351964-hduv0ur4 16 17 ) ) -RRB- cord-351964-hduv0ur4 16 18 and and CC cord-351964-hduv0ur4 16 19 the the DT cord-351964-hduv0ur4 16 20 secreted secrete VBN cord-351964-hduv0ur4 16 21 glycoprotein glycoprotein NN cord-351964-hduv0ur4 16 22 laminin laminin NN cord-351964-hduv0ur4 16 23 ( ( -LRB- cord-351964-hduv0ur4 16 24 Burgess Burgess NNP cord-351964-hduv0ur4 16 25 et et NNP cord-351964-hduv0ur4 16 26 al al NNP cord-351964-hduv0ur4 16 27 . . NNP cord-351964-hduv0ur4 16 28 , , , cord-351964-hduv0ur4 16 29 1985 1985 CD cord-351964-hduv0ur4 16 30 ) ) -RRB- cord-351964-hduv0ur4 16 31 are be VBP cord-351964-hduv0ur4 16 32 examples example NNS cord-351964-hduv0ur4 16 33 of of IN cord-351964-hduv0ur4 16 34 proteins protein NNS cord-351964-hduv0ur4 16 35 that that WDT cord-351964-hduv0ur4 16 36 are be VBP cord-351964-hduv0ur4 16 37 exported export VBN cord-351964-hduv0ur4 16 38 via via IN cord-351964-hduv0ur4 16 39 the the DT cord-351964-hduv0ur4 16 40 constitutive constitutive JJ cord-351964-hduv0ur4 16 41 pathway pathway NN cord-351964-hduv0ur4 16 42 . . . cord-351964-hduv0ur4 17 1 On on IN cord-351964-hduv0ur4 17 2 the the DT cord-351964-hduv0ur4 17 3 other other JJ cord-351964-hduv0ur4 17 4 hand hand NN cord-351964-hduv0ur4 17 5 , , , cord-351964-hduv0ur4 17 6 part part NN cord-351964-hduv0ur4 17 7 of of IN cord-351964-hduv0ur4 17 8 the the DT cord-351964-hduv0ur4 17 9 proopiomelanocortin proopiomelanocortin NNS cord-351964-hduv0ur4 17 10 made make VBN cord-351964-hduv0ur4 17 11 in in IN cord-351964-hduv0ur4 17 12 these these DT cord-351964-hduv0ur4 17 13 calls call NNS cord-351964-hduv0ur4 17 14 follows follow VBZ cord-351964-hduv0ur4 17 15 the the DT cord-351964-hduv0ur4 17 16 regulated regulated JJ cord-351964-hduv0ur4 17 17 pathway pathway NN cord-351964-hduv0ur4 17 18 in in IN cord-351964-hduv0ur4 17 19 which which WDT cord-351964-hduv0ur4 17 20 it -PRON- PRP cord-351964-hduv0ur4 17 21 is be VBZ cord-351964-hduv0ur4 17 22 cleaved cleave VBN cord-351964-hduv0ur4 17 23 proteolyrically proteolyrically RB cord-351964-hduv0ur4 17 24 to to TO cord-351964-hduv0ur4 17 25 yield yield VB cord-351964-hduv0ur4 17 26 ACTH ACTH NNP cord-351964-hduv0ur4 17 27 and and CC cord-351964-hduv0ur4 17 28 is be VBZ cord-351964-hduv0ur4 17 29 stored store VBN cord-351964-hduv0ur4 17 30 in in IN cord-351964-hduv0ur4 17 31 secretory secretory JJ cord-351964-hduv0ur4 17 32 granules granule NNS cord-351964-hduv0ur4 17 33 ( ( -LRB- cord-351964-hduv0ur4 17 34 Gumbiner Gumbiner NNP cord-351964-hduv0ur4 17 35 and and CC cord-351964-hduv0ur4 17 36 Kelly Kelly NNP cord-351964-hduv0ur4 17 37 , , , cord-351964-hduv0ur4 17 38 1982 1982 CD cord-351964-hduv0ur4 17 39 ; ; : cord-351964-hduv0ur4 17 40 Moore Moore NNP cord-351964-hduv0ur4 17 41 and and CC cord-351964-hduv0ur4 17 42 Kelly Kelly NNP cord-351964-hduv0ur4 17 43 , , , cord-351964-hduv0ur4 17 44 1985 1985 CD cord-351964-hduv0ur4 17 45 ) ) -RRB- cord-351964-hduv0ur4 17 46 . . . cord-351964-hduv0ur4 18 1 Foreign foreign JJ cord-351964-hduv0ur4 18 2 peptide peptide NN cord-351964-hduv0ur4 18 3 hormones hormone NNS cord-351964-hduv0ur4 18 4 , , , cord-351964-hduv0ur4 18 5 specified specify VBN cord-351964-hduv0ur4 18 6 by by IN cord-351964-hduv0ur4 18 7 cloned clone VBN cord-351964-hduv0ur4 18 8 genes gene NNS cord-351964-hduv0ur4 18 9 from from IN cord-351964-hduv0ur4 18 10 various various JJ cord-351964-hduv0ur4 18 11 endocrine endocrine JJ cord-351964-hduv0ur4 18 12 and and CC cord-351964-hduv0ur4 18 13 exocrine exocrine JJ cord-351964-hduv0ur4 18 14 cells cell NNS cord-351964-hduv0ur4 18 15 transfected transfecte VBN cord-351964-hduv0ur4 18 16 into into IN cord-351964-hduv0ur4 18 17 AtT20 AtT20 NNP cord-351964-hduv0ur4 18 18 cells cell NNS cord-351964-hduv0ur4 18 19 , , , cord-351964-hduv0ur4 18 20 are be VBP cord-351964-hduv0ur4 18 21 sorted sort VBN cord-351964-hduv0ur4 18 22 into into IN cord-351964-hduv0ur4 18 23 the the DT cord-351964-hduv0ur4 18 24 secretory secretory JJ cord-351964-hduv0ur4 18 25 granules granule NNS cord-351964-hduv0ur4 18 26 of of IN cord-351964-hduv0ur4 18 27 the the DT cord-351964-hduv0ur4 18 28 regulated regulated JJ cord-351964-hduv0ur4 18 29 pathway pathway NN cord-351964-hduv0ur4 18 30 ( ( -LRB- cord-351964-hduv0ur4 18 31 reviewed review VBN cord-351964-hduv0ur4 18 32 in in IN cord-351964-hduv0ur4 18 33 Kelly Kelly NNP cord-351964-hduv0ur4 18 34 , , , cord-351964-hduv0ur4 18 35 1985 1985 CD cord-351964-hduv0ur4 18 36 ) ) -RRB- cord-351964-hduv0ur4 18 37 . . . cord-351964-hduv0ur4 19 1 Condensed condense VBN cord-351964-hduv0ur4 19 2 secretory secretory NN cord-351964-hduv0ur4 19 3 proteins protein NNS cord-351964-hduv0ur4 19 4 first first RB cord-351964-hduv0ur4 19 5 accumulate accumulate VBP cord-351964-hduv0ur4 19 6 in in IN cord-351964-hduv0ur4 19 7 the the DT cord-351964-hduv0ur4 19 8 trans trans NN cord-351964-hduv0ur4 19 9 - - JJ cord-351964-hduv0ur4 19 10 most most JJS cord-351964-hduv0ur4 19 11 Golgi Golgi NNP cord-351964-hduv0ur4 19 12 compartment compartment NN cord-351964-hduv0ur4 19 13 of of IN cord-351964-hduv0ur4 19 14 AtT20 AtT20 NNP cord-351964-hduv0ur4 19 15 cells cell NNS cord-351964-hduv0ur4 19 16 . . . cord-351964-hduv0ur4 20 1 This this DT cord-351964-hduv0ur4 20 2 extreme extreme JJ cord-351964-hduv0ur4 20 3 trans trans NN cord-351964-hduv0ur4 20 4 - - NN cord-351964-hduv0ur4 20 5 compartment compartment NNP cord-351964-hduv0ur4 20 6 , , , cord-351964-hduv0ur4 20 7 which which WDT cord-351964-hduv0ur4 20 8 lies lie VBZ cord-351964-hduv0ur4 20 9 beyond beyond IN cord-351964-hduv0ur4 20 10 the the DT cord-351964-hduv0ur4 20 11 TPPase TPPase NNP cord-351964-hduv0ur4 20 12 - - HYPH cord-351964-hduv0ur4 20 13 positive positive JJ cord-351964-hduv0ur4 20 14 cisternae cisternae NN cord-351964-hduv0ur4 20 15 of of IN cord-351964-hduv0ur4 20 16 the the DT cord-351964-hduv0ur4 20 17 Golgi Golgi NNP cord-351964-hduv0ur4 20 18 stack stack NN cord-351964-hduv0ur4 20 19 and and CC cord-351964-hduv0ur4 20 20 has have VBZ cord-351964-hduv0ur4 20 21 been be VBN cord-351964-hduv0ur4 20 22 termed term VBN cord-351964-hduv0ur4 20 23 the the DT cord-351964-hduv0ur4 20 24 trans trans NNP cord-351964-hduv0ur4 20 25 - - NNP cord-351964-hduv0ur4 20 26 Golgi Golgi NNP cord-351964-hduv0ur4 20 27 network network NN cord-351964-hduv0ur4 20 28 or or CC cord-351964-hduv0ur4 20 29 trans trans JJ cord-351964-hduv0ur4 20 30 - - JJ cord-351964-hduv0ur4 20 31 Golgi Golgi NNP cord-351964-hduv0ur4 20 32 reticulum reticulum NN cord-351964-hduv0ur4 20 33 ( ( -LRB- cord-351964-hduv0ur4 20 34 Griffiths Griffiths NNP cord-351964-hduv0ur4 20 35 and and CC cord-351964-hduv0ur4 20 36 Simons Simons NNP cord-351964-hduv0ur4 20 37 , , , cord-351964-hduv0ur4 20 38 1986 1986 CD cord-351964-hduv0ur4 20 39 ) ) -RRB- cord-351964-hduv0ur4 20 40 , , , cord-351964-hduv0ur4 20 41 is be VBZ cord-351964-hduv0ur4 20 42 the the DT cord-351964-hduv0ur4 20 43 site site NN cord-351964-hduv0ur4 20 44 of of IN cord-351964-hduv0ur4 20 45 formation formation NN cord-351964-hduv0ur4 20 46 of of IN cord-351964-hduv0ur4 20 47 the the DT cord-351964-hduv0ur4 20 48 secretory secretory JJ cord-351964-hduv0ur4 20 49 granules granule NNS cord-351964-hduv0ur4 20 50 of of IN cord-351964-hduv0ur4 20 51 the the DT cord-351964-hduv0ur4 20 52 regulated regulated JJ cord-351964-hduv0ur4 20 53 pathway pathway NN cord-351964-hduv0ur4 20 54 . . . cord-351964-hduv0ur4 21 1 The the DT cord-351964-hduv0ur4 21 2 corona corona NN cord-351964-hduv0ur4 21 3 ~ ~ NFP cord-351964-hduv0ur4 21 4 Arus Arus NNP cord-351964-hduv0ur4 21 5 mouse mouse NN cord-351964-hduv0ur4 21 6 hepatitis hepatitis NN cord-351964-hduv0ur4 21 7 virus virus NN cord-351964-hduv0ur4 21 8 strain strain VBP cord-351964-hduv0ur4 21 9 A59 A59 NNP cord-351964-hduv0ur4 22 1 ( ( -LRB- cord-351964-hduv0ur4 22 2 MHV MHV NNP cord-351964-hduv0ur4 22 3 - - HYPH cord-351964-hduv0ur4 22 4 A59 A59 NNP cord-351964-hduv0ur4 22 5 ) ) -RRB- cord-351964-hduv0ur4 23 1 I -PRON- PRP cord-351964-hduv0ur4 23 2 infects infect VBZ cord-351964-hduv0ur4 23 3 and and CC cord-351964-hduv0ur4 23 4 replicates replicate VBZ cord-351964-hduv0ur4 23 5 efficiently efficiently RB cord-351964-hduv0ur4 23 6 in in IN cord-351964-hduv0ur4 23 7 some some DT cord-351964-hduv0ur4 23 8 mouse mouse NN cord-351964-hduv0ur4 23 9 1 1 CD cord-351964-hduv0ur4 23 10 . . . cord-351964-hduv0ur4 24 1 Abbre Abbre NNP cord-351964-hduv0ur4 24 2 ~ ~ CD cord-351964-hduv0ur4 24 3 ations ation NNS cord-351964-hduv0ur4 24 4 used use VBN cord-351964-hduv0ur4 24 5 in in IN cord-351964-hduv0ur4 24 6 this this DT cord-351964-hduv0ur4 24 7 paper paper NN cord-351964-hduv0ur4 24 8 : : : cord-351964-hduv0ur4 24 9 ECM ECM NNP cord-351964-hduv0ur4 24 10 , , , cord-351964-hduv0ur4 24 11 extracellular extracellular JJ cord-351964-hduv0ur4 24 12 matrix matrix NN cord-351964-hduv0ur4 24 13 ; ; : cord-351964-hduv0ur4 24 14 MHV MHV NNP cord-351964-hduv0ur4 24 15 - - HYPH cord-351964-hduv0ur4 24 16 A59 A59 NNP cord-351964-hduv0ur4 24 17 , , , cord-351964-hduv0ur4 24 18 mouse mouse NN cord-351964-hduv0ur4 24 19 hepatitis hepatitis NN cord-351964-hduv0ur4 24 20 virus virus NN cord-351964-hduv0ur4 24 21 A59 A59 NNP cord-351964-hduv0ur4 24 22 ; ; : cord-351964-hduv0ur4 24 23 PI pi NN cord-351964-hduv0ur4 24 24 , , , cord-351964-hduv0ur4 24 25 protease protease NN cord-351964-hduv0ur4 24 26 inhibitor inhibitor NN cord-351964-hduv0ur4 24 27 cocktail cocktail NN cord-351964-hduv0ur4 24 28 ( ( -LRB- cord-351964-hduv0ur4 24 29 1 1 CD cord-351964-hduv0ur4 24 30 Ig Ig NNP cord-351964-hduv0ur4 24 31 / / SYM cord-351964-hduv0ur4 24 32 ml ml IN cord-351964-hduv0ur4 24 33 leupeptin leupeptin NNP cord-351964-hduv0ur4 24 34 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 24 35 cell cell NN cord-351964-hduv0ur4 24 36 lines line NNS cord-351964-hduv0ur4 24 37 in in IN cord-351964-hduv0ur4 24 38 which which WDT cord-351964-hduv0ur4 24 39 the the DT cord-351964-hduv0ur4 24 40 maximum maximum JJ cord-351964-hduv0ur4 24 41 rate rate NN cord-351964-hduv0ur4 24 42 of of IN cord-351964-hduv0ur4 24 43 release release NN cord-351964-hduv0ur4 24 44 of of IN cord-351964-hduv0ur4 24 45 progeny progeny JJ cord-351964-hduv0ur4 24 46 virions virion NNS cord-351964-hduv0ur4 24 47 occurs occur VBZ cord-351964-hduv0ur4 24 48 well well RB cord-351964-hduv0ur4 24 49 before before IN cord-351964-hduv0ur4 24 50 cell cell NN cord-351964-hduv0ur4 24 51 lysis lysis NN cord-351964-hduv0ur4 24 52 . . . cord-351964-hduv0ur4 25 1 The the DT cord-351964-hduv0ur4 25 2 virus virus NN cord-351964-hduv0ur4 25 3 , , , cord-351964-hduv0ur4 25 4 which which WDT cord-351964-hduv0ur4 25 5 buds bud VBZ cord-351964-hduv0ur4 25 6 into into IN cord-351964-hduv0ur4 25 7 pre pre JJ cord-351964-hduv0ur4 25 8 - - JJ cord-351964-hduv0ur4 25 9 Golgi golgi JJ cord-351964-hduv0ur4 25 10 compartments compartment NNS cord-351964-hduv0ur4 25 11 , , , cord-351964-hduv0ur4 25 12 moves move VBZ cord-351964-hduv0ur4 25 13 through through IN cord-351964-hduv0ur4 25 14 the the DT cord-351964-hduv0ur4 25 15 Golgi Golgi NNP cord-351964-hduv0ur4 25 16 cisternae cisternae NN cord-351964-hduv0ur4 25 17 and and CC cord-351964-hduv0ur4 25 18 exploits exploit VBZ cord-351964-hduv0ur4 25 19 vesicles vesicle NNS cord-351964-hduv0ur4 25 20 of of IN cord-351964-hduv0ur4 25 21 the the DT cord-351964-hduv0ur4 25 22 constitutive constitutive JJ cord-351964-hduv0ur4 25 23 exocytic exocytic JJ cord-351964-hduv0ur4 25 24 pathway pathway NN cord-351964-hduv0ur4 25 25 of of IN cord-351964-hduv0ur4 25 26 these these DT cord-351964-hduv0ur4 25 27 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 25 28 hosts host NNS cord-351964-hduv0ur4 25 29 , , , cord-351964-hduv0ur4 25 30 which which WDT cord-351964-hduv0ur4 25 31 lack lack VBP cord-351964-hduv0ur4 25 32 a a DT cord-351964-hduv0ur4 25 33 regulated regulated JJ cord-351964-hduv0ur4 25 34 pathway pathway NN cord-351964-hduv0ur4 25 35 , , , cord-351964-hduv0ur4 25 36 to to TO cord-351964-hduv0ur4 25 37 reach reach VB cord-351964-hduv0ur4 25 38 the the DT cord-351964-hduv0ur4 25 39 cell cell NN cord-351964-hduv0ur4 25 40 surface surface NN cord-351964-hduv0ur4 25 41 ; ; : cord-351964-hduv0ur4 25 42 the the DT cord-351964-hduv0ur4 25 43 post post JJ cord-351964-hduv0ur4 25 44 - - JJ cord-351964-hduv0ur4 25 45 Golgi golgi JJ cord-351964-hduv0ur4 25 46 vesicles vesicle NNS cord-351964-hduv0ur4 25 47 filled fill VBN cord-351964-hduv0ur4 25 48 with with IN cord-351964-hduv0ur4 25 49 progeny progeny JJ cord-351964-hduv0ur4 25 50 virions virion NNS cord-351964-hduv0ur4 25 51 that that WDT cord-351964-hduv0ur4 25 52 can can MD cord-351964-hduv0ur4 25 53 be be VB cord-351964-hduv0ur4 25 54 seen see VBN cord-351964-hduv0ur4 25 55 near near IN cord-351964-hduv0ur4 25 56 the the DT cord-351964-hduv0ur4 25 57 cell cell NN cord-351964-hduv0ur4 25 58 surface surface NN cord-351964-hduv0ur4 25 59 apparently apparently RB cord-351964-hduv0ur4 25 60 fuse fuse VBP cord-351964-hduv0ur4 25 61 with with IN cord-351964-hduv0ur4 25 62 the the DT cord-351964-hduv0ur4 25 63 plasma plasma NN cord-351964-hduv0ur4 25 64 membrane membrane NN cord-351964-hduv0ur4 25 65 to to TO cord-351964-hduv0ur4 25 66 release release VB cord-351964-hduv0ur4 25 67 the the DT cord-351964-hduv0ur4 25 68 virus virus NN cord-351964-hduv0ur4 25 69 into into IN cord-351964-hduv0ur4 25 70 the the DT cord-351964-hduv0ur4 25 71 medium medium NN cord-351964-hduv0ur4 25 72 ( ( -LRB- cord-351964-hduv0ur4 25 73 for for IN cord-351964-hduv0ur4 25 74 review review NN cord-351964-hduv0ur4 25 75 see see VB cord-351964-hduv0ur4 25 76 Dubois Dubois NNP cord-351964-hduv0ur4 25 77 - - HYPH cord-351964-hduv0ur4 25 78 Dalcq Dalcq NNP cord-351964-hduv0ur4 25 79 et et NNP cord-351964-hduv0ur4 25 80 al al NNP cord-351964-hduv0ur4 25 81 . . NNP cord-351964-hduv0ur4 25 82 , , , cord-351964-hduv0ur4 25 83 1984 1984 CD cord-351964-hduv0ur4 25 84 ) ) -RRB- cord-351964-hduv0ur4 25 85 . . . cord-351964-hduv0ur4 26 1 MHV MHV NNP cord-351964-hduv0ur4 26 2 - - HYPH cord-351964-hduv0ur4 26 3 A59 A59 NNP cord-351964-hduv0ur4 26 4 can can MD cord-351964-hduv0ur4 26 5 , , , cord-351964-hduv0ur4 26 6 however however RB cord-351964-hduv0ur4 26 7 , , , cord-351964-hduv0ur4 26 8 also also RB cord-351964-hduv0ur4 26 9 infect infect VBP cord-351964-hduv0ur4 26 10 AtT20 AtT20 NNP cord-351964-hduv0ur4 26 11 cells cell NNS cord-351964-hduv0ur4 26 12 inefficiently inefficiently RB cord-351964-hduv0ur4 26 13 ( ( -LRB- cord-351964-hduv0ur4 26 14 Tooze Tooze NNP cord-351964-hduv0ur4 26 15 and and CC cord-351964-hduv0ur4 26 16 Tooze Tooze NNP cord-351964-hduv0ur4 26 17 , , , cord-351964-hduv0ur4 26 18 1985 1985 CD cord-351964-hduv0ur4 26 19 ) ) -RRB- cord-351964-hduv0ur4 26 20 . . . cord-351964-hduv0ur4 27 1 In in IN cord-351964-hduv0ur4 27 2 AtT20 AtT20 NNP cord-351964-hduv0ur4 27 3 cells cell NNS cord-351964-hduv0ur4 27 4 , , , cord-351964-hduv0ur4 27 5 as as IN cord-351964-hduv0ur4 27 6 in in IN cord-351964-hduv0ur4 27 7 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 27 8 cells cell NNS cord-351964-hduv0ur4 27 9 , , , cord-351964-hduv0ur4 27 10 the the DT cord-351964-hduv0ur4 27 11 virus virus NN cord-351964-hduv0ur4 27 12 buds bud VBZ cord-351964-hduv0ur4 27 13 into into IN cord-351964-hduv0ur4 27 14 pre pre JJ cord-351964-hduv0ur4 27 15 - - JJ cord-351964-hduv0ur4 27 16 Golgi golgi JJ cord-351964-hduv0ur4 27 17 compartments compartment NNS cord-351964-hduv0ur4 27 18 . . . cord-351964-hduv0ur4 28 1 The the DT cord-351964-hduv0ur4 28 2 progeny progeny NN cord-351964-hduv0ur4 28 3 virions virion NNS cord-351964-hduv0ur4 28 4 then then RB cord-351964-hduv0ur4 28 5 traverse traverse VB cord-351964-hduv0ur4 28 6 the the DT cord-351964-hduv0ur4 28 7 Golgi Golgi NNP cord-351964-hduv0ur4 28 8 stacks stack NNS cord-351964-hduv0ur4 28 9 and and CC cord-351964-hduv0ur4 28 10 are be VBP cord-351964-hduv0ur4 28 11 transported transport VBN cord-351964-hduv0ur4 28 12 thence thence RB cord-351964-hduv0ur4 28 13 to to IN cord-351964-hduv0ur4 28 14 the the DT cord-351964-hduv0ur4 28 15 cell cell NN cord-351964-hduv0ur4 28 16 surface surface NN cord-351964-hduv0ur4 28 17 . . . cord-351964-hduv0ur4 29 1 Assuming assume VBG cord-351964-hduv0ur4 29 2 that that IN cord-351964-hduv0ur4 29 3 MHV MHV NNP cord-351964-hduv0ur4 29 4 - - HYPH cord-351964-hduv0ur4 29 5 A59 A59 NNP cord-351964-hduv0ur4 29 6 utilizes utilize VBZ cord-351964-hduv0ur4 29 7 the the DT cord-351964-hduv0ur4 29 8 constitutive constitutive JJ cord-351964-hduv0ur4 29 9 pathway pathway NN cord-351964-hduv0ur4 29 10 in in IN cord-351964-hduv0ur4 29 11 AtT20 AtT20 NNP cord-351964-hduv0ur4 29 12 cells cell NNS cord-351964-hduv0ur4 29 13 , , , cord-351964-hduv0ur4 29 14 as as IN cord-351964-hduv0ur4 29 15 it -PRON- PRP cord-351964-hduv0ur4 29 16 does do VBZ cord-351964-hduv0ur4 29 17 in in IN cord-351964-hduv0ur4 29 18 fibroblasts fibroblast NNS cord-351964-hduv0ur4 29 19 , , , cord-351964-hduv0ur4 29 20 we -PRON- PRP cord-351964-hduv0ur4 29 21 can can MD cord-351964-hduv0ur4 29 22 use use VB cord-351964-hduv0ur4 29 23 the the DT cord-351964-hduv0ur4 29 24 viral viral JJ cord-351964-hduv0ur4 29 25 particles particle NNS cord-351964-hduv0ur4 29 26 as as IN cord-351964-hduv0ur4 29 27 a a DT cord-351964-hduv0ur4 29 28 morphological morphological JJ cord-351964-hduv0ur4 29 29 marker marker NN cord-351964-hduv0ur4 29 30 for for IN cord-351964-hduv0ur4 29 31 that that DT cord-351964-hduv0ur4 29 32 pathway pathway NN cord-351964-hduv0ur4 29 33 and and CC cord-351964-hduv0ur4 29 34 use use VB cord-351964-hduv0ur4 29 35 condensing condense VBG cord-351964-hduv0ur4 29 36 secretory secretory JJ cord-351964-hduv0ur4 29 37 proteins protein NNS cord-351964-hduv0ur4 29 38 , , , cord-351964-hduv0ur4 29 39 including include VBG cord-351964-hduv0ur4 29 40 ACTH ACTH NNP cord-351964-hduv0ur4 29 41 , , , cord-351964-hduv0ur4 29 42 as as IN cord-351964-hduv0ur4 29 43 a a DT cord-351964-hduv0ur4 29 44 morphological morphological JJ cord-351964-hduv0ur4 29 45 marker marker NN cord-351964-hduv0ur4 29 46 for for IN cord-351964-hduv0ur4 29 47 the the DT cord-351964-hduv0ur4 29 48 regulated regulated JJ cord-351964-hduv0ur4 29 49 pathway pathway NN cord-351964-hduv0ur4 29 50 . . . cord-351964-hduv0ur4 30 1 We -PRON- PRP cord-351964-hduv0ur4 30 2 can can MD cord-351964-hduv0ur4 30 3 then then RB cord-351964-hduv0ur4 30 4 ask ask VB cord-351964-hduv0ur4 30 5 at at IN cord-351964-hduv0ur4 30 6 what what WDT cord-351964-hduv0ur4 30 7 level level NN cord-351964-hduv0ur4 30 8 do do VBP cord-351964-hduv0ur4 30 9 the the DT cord-351964-hduv0ur4 30 10 two two CD cord-351964-hduv0ur4 30 11 pathways pathway NNS cord-351964-hduv0ur4 30 12 diverge diverge VBP cord-351964-hduv0ur4 30 13 ? ? . cord-351964-hduv0ur4 31 1 Which which WDT cord-351964-hduv0ur4 31 2 is be VBZ cord-351964-hduv0ur4 31 3 the the DT cord-351964-hduv0ur4 31 4 last last JJ cord-351964-hduv0ur4 31 5 compartment compartment NN cord-351964-hduv0ur4 31 6 to to TO cord-351964-hduv0ur4 31 7 contain contain VB cord-351964-hduv0ur4 31 8 both both DT cord-351964-hduv0ur4 31 9 virions virion NNS cord-351964-hduv0ur4 31 10 and and CC cord-351964-hduv0ur4 31 11 condensing condense VBG cord-351964-hduv0ur4 31 12 secretory secretory NN cord-351964-hduv0ur4 31 13 in in IN cord-351964-hduv0ur4 31 14 distilled distil VBN cord-351964-hduv0ur4 31 15 HzO hzo NN cord-351964-hduv0ur4 31 16 , , , cord-351964-hduv0ur4 31 17 1 1 CD cord-351964-hduv0ur4 31 18 1.tg/ml 1.tg/ml CD cord-351964-hduv0ur4 31 19 antipain antipain NN cord-351964-hduv0ur4 31 20 in in IN cord-351964-hduv0ur4 31 21 distilled distil VBN cord-351964-hduv0ur4 31 22 H20 H20 NNP cord-351964-hduv0ur4 31 23 , , , cord-351964-hduv0ur4 31 24 1 1 CD cord-351964-hduv0ur4 31 25 p.g p.g NNP cord-351964-hduv0ur4 31 26 / / SYM cord-351964-hduv0ur4 31 27 ml ml CC cord-351964-hduv0ur4 31 28 pepstatin pepstatin NNP cord-351964-hduv0ur4 31 29 in in IN cord-351964-hduv0ur4 31 30 H20 H20 NNP cord-351964-hduv0ur4 31 31 , , , cord-351964-hduv0ur4 31 32 1 1 CD cord-351964-hduv0ur4 31 33 mM mm CD cord-351964-hduv0ur4 31 34 bertzamidine bertzamidine NN cord-351964-hduv0ur4 31 35 in in IN cord-351964-hduv0ur4 31 36 dimethylsulfoxide dimethylsulfoxide NN cord-351964-hduv0ur4 31 37 , , , cord-351964-hduv0ur4 31 38 40 40 CD cord-351964-hduv0ur4 31 39 gtg gtg NN cord-351964-hduv0ur4 32 1 /ml /ml NFP cord-351964-hduv0ur4 32 2 phenylmethylsulfonyl phenylmethylsulfonyl NNP cord-351964-hduv0ur4 32 3 fluoride fluoride NN cord-351964-hduv0ur4 32 4 in in IN cord-351964-hduv0ur4 32 5 anhydrous anhydrous JJ cord-351964-hduv0ur4 32 6 ethanol ethanol NN cord-351964-hduv0ur4 32 7 , , , cord-351964-hduv0ur4 32 8 and and CC cord-351964-hduv0ur4 32 9 10 10 CD cord-351964-hduv0ur4 32 10 U u NN cord-351964-hduv0ur4 32 11 / / SYM cord-351964-hduv0ur4 32 12 ml ml NN cord-351964-hduv0ur4 32 13 trasylol trasylol NNP cord-351964-hduv0ur4 32 14 ) ) -RRB- cord-351964-hduv0ur4 32 15 ; ; : cord-351964-hduv0ur4 32 16 RIPA RIPA NNP cord-351964-hduv0ur4 32 17 , , , cord-351964-hduv0ur4 32 18 immunoprecipitation immunoprecipitation NN cord-351964-hduv0ur4 32 19 buffer buffer NN cord-351964-hduv0ur4 32 20 ( ( -LRB- cord-351964-hduv0ur4 32 21 1 1 CD cord-351964-hduv0ur4 32 22 % % NN cord-351964-hduv0ur4 32 23 [ [ -LRB- cord-351964-hduv0ur4 32 24 wt wt NN cord-351964-hduv0ur4 32 25 / / SYM cord-351964-hduv0ur4 32 26 vol vol NN cord-351964-hduv0ur4 32 27 ] ] -RRB- cord-351964-hduv0ur4 32 28 TX-100 TX-100 NNP cord-351964-hduv0ur4 32 29 , , , cord-351964-hduv0ur4 32 30 0.1 0.1 CD cord-351964-hduv0ur4 32 31 % % NN cord-351964-hduv0ur4 32 32 lwt lwt NNP cord-351964-hduv0ur4 32 33 / / SYM cord-351964-hduv0ur4 32 34 vol vol NNP cord-351964-hduv0ur4 32 35 ] ] -RRB- cord-351964-hduv0ur4 32 36 SDS SDS NNP cord-351964-hduv0ur4 32 37 , , , cord-351964-hduv0ur4 32 38 0.15 0.15 CD cord-351964-hduv0ur4 32 39 M M NNP cord-351964-hduv0ur4 32 40 NaC1 NaC1 NNP cord-351964-hduv0ur4 32 41 , , , cord-351964-hduv0ur4 32 42 1 1 CD cord-351964-hduv0ur4 32 43 % % NN cord-351964-hduv0ur4 32 44 [ [ -LRB- cord-351964-hduv0ur4 32 45 wt wt NN cord-351964-hduv0ur4 32 46 / / SYM cord-351964-hduv0ur4 32 47 vol vol NNP cord-351964-hduv0ur4 32 48 ] ] -RRB- cord-351964-hduv0ur4 32 49 sodium sodium NNP cord-351964-hduv0ur4 32 50 dodeeylate dodeeylate NNP cord-351964-hduv0ur4 32 51 , , , cord-351964-hduv0ur4 33 1 10ram 10ram NNP cord-351964-hduv0ur4 33 2 Tris Tris NNP cord-351964-hduv0ur4 33 3 , , , cord-351964-hduv0ur4 33 4 pH pH NNP cord-351964-hduv0ur4 33 5 7.6 7.6 CD cord-351964-hduv0ur4 33 6 ) ) -RRB- cord-351964-hduv0ur4 33 7 ; ; : cord-351964-hduv0ur4 33 8 VSV VSV NNP cord-351964-hduv0ur4 33 9 , , , cord-351964-hduv0ur4 33 10 vesicular vesicular JJ cord-351964-hduv0ur4 33 11 stomatitis stomatitis NN cord-351964-hduv0ur4 33 12 virus virus NN cord-351964-hduv0ur4 33 13 . . . cord-351964-hduv0ur4 34 1 proteins protein NNS cord-351964-hduv0ur4 34 2 and and CC cord-351964-hduv0ur4 34 3 are be VBP cord-351964-hduv0ur4 34 4 the the DT cord-351964-hduv0ur4 34 5 two two CD cord-351964-hduv0ur4 34 6 markers marker NNS cord-351964-hduv0ur4 34 7 segregated segregate VBN cord-351964-hduv0ur4 34 8 into into IN cord-351964-hduv0ur4 34 9 different different JJ cord-351964-hduv0ur4 34 10 populations population NNS cord-351964-hduv0ur4 34 11 of of IN cord-351964-hduv0ur4 34 12 transport transport NN cord-351964-hduv0ur4 34 13 vesicles vesicle NNS cord-351964-hduv0ur4 34 14 at at IN cord-351964-hduv0ur4 34 15 the the DT cord-351964-hduv0ur4 34 16 exit exit NN cord-351964-hduv0ur4 34 17 from from IN cord-351964-hduv0ur4 34 18 this this DT cord-351964-hduv0ur4 34 19 compartment compartment NN cord-351964-hduv0ur4 34 20 ? ? . cord-351964-hduv0ur4 35 1 We -PRON- PRP cord-351964-hduv0ur4 35 2 report report VBP cord-351964-hduv0ur4 35 3 here here RB cord-351964-hduv0ur4 35 4 our -PRON- PRP$ cord-351964-hduv0ur4 35 5 attempts attempt NNS cord-351964-hduv0ur4 35 6 to to TO cord-351964-hduv0ur4 35 7 answer answer VB cord-351964-hduv0ur4 35 8 these these DT cord-351964-hduv0ur4 35 9 questions question NNS cord-351964-hduv0ur4 35 10 by by IN cord-351964-hduv0ur4 35 11 electron electron NN cord-351964-hduv0ur4 35 12 microscopy microscopy NNP cord-351964-hduv0ur4 35 13 and and CC cord-351964-hduv0ur4 35 14 immunocytochemistry immunocytochemistry NN cord-351964-hduv0ur4 35 15 . . . cord-351964-hduv0ur4 36 1 Our -PRON- PRP$ cord-351964-hduv0ur4 36 2 observations observation NNS cord-351964-hduv0ur4 36 3 indicate indicate VBP cord-351964-hduv0ur4 36 4 that that IN cord-351964-hduv0ur4 36 5 the the DT cord-351964-hduv0ur4 36 6 constitutive constitutive JJ cord-351964-hduv0ur4 36 7 and and CC cord-351964-hduv0ur4 36 8 regulated regulated JJ cord-351964-hduv0ur4 36 9 pathways pathway NNS cord-351964-hduv0ur4 36 10 in in IN cord-351964-hduv0ur4 36 11 AtT20 AtT20 NNP cord-351964-hduv0ur4 36 12 cells cell NNS cord-351964-hduv0ur4 36 13 do do VBP cord-351964-hduv0ur4 36 14 indeed indeed RB cord-351964-hduv0ur4 36 15 diverge diverge VB cord-351964-hduv0ur4 36 16 at at IN cord-351964-hduv0ur4 36 17 the the DT cord-351964-hduv0ur4 36 18 exit exit NN cord-351964-hduv0ur4 36 19 from from IN cord-351964-hduv0ur4 36 20 the the DT cord-351964-hduv0ur4 36 21 trans trans NNP cord-351964-hduv0ur4 36 22 - - NNP cord-351964-hduv0ur4 36 23 Golgi Golgi NNP cord-351964-hduv0ur4 36 24 network network NN cord-351964-hduv0ur4 36 25 . . . cord-351964-hduv0ur4 37 1 ART20 art20 VB cord-351964-hduv0ur4 38 1 D16V d16v NN cord-351964-hduv0ur4 38 2 cells cell NNS cord-351964-hduv0ur4 38 3 were be VBD cord-351964-hduv0ur4 38 4 propagated propagate VBN cord-351964-hduv0ur4 38 5 at at IN cord-351964-hduv0ur4 38 6 37~ 37~ CD cord-351964-hduv0ur4 38 7 in in IN cord-351964-hduv0ur4 38 8 a a DT cord-351964-hduv0ur4 38 9 5 5 CD cord-351964-hduv0ur4 38 10 % % NN cord-351964-hduv0ur4 38 11 COz COz NNS cord-351964-hduv0ur4 38 12 incubator incubator NN cord-351964-hduv0ur4 38 13 as as IN cord-351964-hduv0ur4 38 14 previously previously RB cord-351964-hduv0ur4 38 15 described describe VBN cord-351964-hduv0ur4 38 16 ( ( -LRB- cord-351964-hduv0ur4 38 17 Tooze Tooze NNP cord-351964-hduv0ur4 38 18 and and CC cord-351964-hduv0ur4 38 19 Tooze Tooze NNP cord-351964-hduv0ur4 38 20 , , , cord-351964-hduv0ur4 38 21 1985 1985 CD cord-351964-hduv0ur4 38 22 ) ) -RRB- cord-351964-hduv0ur4 38 23 in in IN cord-351964-hduv0ur4 38 24 DME DME NNP cord-351964-hduv0ur4 38 25 supplemented supplement VBN cord-351964-hduv0ur4 38 26 with with IN cord-351964-hduv0ur4 38 27 10 10 CD cord-351964-hduv0ur4 38 28 % % NN cord-351964-hduv0ur4 38 29 horse horse NN cord-351964-hduv0ur4 38 30 serum serum NN cord-351964-hduv0ur4 38 31 and and CC cord-351964-hduv0ur4 38 32 4.5 4.5 CD cord-351964-hduv0ur4 38 33 g g NN cord-351964-hduv0ur4 38 34 / / SYM cord-351964-hduv0ur4 38 35 liter liter NN cord-351964-hduv0ur4 38 36 of of IN cord-351964-hduv0ur4 38 37 glucose glucose NN cord-351964-hduv0ur4 38 38 . . . cord-351964-hduv0ur4 39 1 When when WRB cord-351964-hduv0ur4 39 2 propagated propagate VBN cord-351964-hduv0ur4 39 3 on on IN cord-351964-hduv0ur4 39 4 normal normal JJ cord-351964-hduv0ur4 39 5 tissue tissue NN cord-351964-hduv0ur4 39 6 culture culture NN cord-351964-hduv0ur4 39 7 plastic plastic NN cord-351964-hduv0ur4 39 8 surfaces surface NNS cord-351964-hduv0ur4 39 9 or or CC cord-351964-hduv0ur4 39 10 glass glass NN cord-351964-hduv0ur4 39 11 AtT20 AtT20 NNP cord-351964-hduv0ur4 39 12 cells cell NNS cord-351964-hduv0ur4 39 13 form form VBP cord-351964-hduv0ur4 39 14 dense dense JJ cord-351964-hduv0ur4 39 15 clumps clump VBZ cord-351964-hduv0ur4 39 16 many many JJ cord-351964-hduv0ur4 39 17 ceils ceil NNS cord-351964-hduv0ur4 39 18 deep deep RB cord-351964-hduv0ur4 39 19 . . . cord-351964-hduv0ur4 40 1 When when WRB cord-351964-hduv0ur4 40 2 plated plate VBN cord-351964-hduv0ur4 40 3 on on IN cord-351964-hduv0ur4 40 4 plastic plastic NN cord-351964-hduv0ur4 40 5 or or CC cord-351964-hduv0ur4 40 6 glass glass NN cord-351964-hduv0ur4 40 7 surfaces surface NNS cord-351964-hduv0ur4 40 8 coated coat VBN cord-351964-hduv0ur4 40 9 with with IN cord-351964-hduv0ur4 40 10 extracellular extracellular JJ cord-351964-hduv0ur4 40 11 matrix matrix NN cord-351964-hduv0ur4 40 12 ( ( -LRB- cord-351964-hduv0ur4 40 13 ECM ECM NNP cord-351964-hduv0ur4 40 14 ; ; : cord-351964-hduv0ur4 40 15 International International NNP cord-351964-hduv0ur4 40 16 Bio Bio NNP cord-351964-hduv0ur4 40 17 - - HYPH cord-351964-hduv0ur4 40 18 Technologies Technologies NNPS cord-351964-hduv0ur4 40 19 Ltd. Ltd. NNP cord-351964-hduv0ur4 40 20 , , , cord-351964-hduv0ur4 40 21 Jerusalem Jerusalem NNP cord-351964-hduv0ur4 40 22 , , , cord-351964-hduv0ur4 40 23 Israel Israel NNP cord-351964-hduv0ur4 40 24 ) ) -RRB- cord-351964-hduv0ur4 40 25 , , , cord-351964-hduv0ur4 40 26 the the DT cord-351964-hduv0ur4 40 27 AtT20 AtT20 NNP cord-351964-hduv0ur4 40 28 cells cell NNS cord-351964-hduv0ur4 40 29 adhere adhere VBP cord-351964-hduv0ur4 40 30 and and CC cord-351964-hduv0ur4 40 31 flatten flatten VB cord-351964-hduv0ur4 40 32 within within IN cord-351964-hduv0ur4 40 33 1 1 CD cord-351964-hduv0ur4 40 34 h h NN cord-351964-hduv0ur4 40 35 , , , cord-351964-hduv0ur4 40 36 and and CC cord-351964-hduv0ur4 40 37 grow grow VB cord-351964-hduv0ur4 40 38 as as IN cord-351964-hduv0ur4 40 39 a a DT cord-351964-hduv0ur4 40 40 monolayer monolayer NN cord-351964-hduv0ur4 40 41 . . . cord-351964-hduv0ur4 41 1 This this DT cord-351964-hduv0ur4 41 2 greatly greatly RB cord-351964-hduv0ur4 41 3 facilitates facilitate VBZ cord-351964-hduv0ur4 41 4 optical optical JJ cord-351964-hduv0ur4 41 5 microscopy microscopy NNS cord-351964-hduv0ur4 41 6 . . . cord-351964-hduv0ur4 42 1 In in IN cord-351964-hduv0ur4 42 2 addition addition NN cord-351964-hduv0ur4 42 3 we -PRON- PRP cord-351964-hduv0ur4 42 4 have have VBP cord-351964-hduv0ur4 42 5 found find VBN cord-351964-hduv0ur4 42 6 that that IN cord-351964-hduv0ur4 42 7 ART20 ART20 NNP cord-351964-hduv0ur4 42 8 cells cell NNS cord-351964-hduv0ur4 42 9 growing grow VBG cord-351964-hduv0ur4 42 10 on on IN cord-351964-hduv0ur4 42 11 ECM ECM NNP cord-351964-hduv0ur4 42 12 am be VBP cord-351964-hduv0ur4 42 13 about about RB cord-351964-hduv0ur4 42 14 five five CD cord-351964-hduv0ur4 42 15 times time NNS cord-351964-hduv0ur4 42 16 more more RBR cord-351964-hduv0ur4 42 17 susceptible susceptible JJ cord-351964-hduv0ur4 42 18 to to IN cord-351964-hduv0ur4 42 19 infection infection NN cord-351964-hduv0ur4 42 20 with with IN cord-351964-hduv0ur4 42 21 the the DT cord-351964-hduv0ur4 42 22 coronavirus coronavirus NN cord-351964-hduv0ur4 42 23 MHV MHV NNP cord-351964-hduv0ur4 42 24 - - HYPH cord-351964-hduv0ur4 42 25 A59 A59 NNP cord-351964-hduv0ur4 42 26 than than IN cord-351964-hduv0ur4 42 27 are be VBP cord-351964-hduv0ur4 42 28 parallel parallel JJ cord-351964-hduv0ur4 42 29 cultures culture NNS cord-351964-hduv0ur4 42 30 growing grow VBG cord-351964-hduv0ur4 42 31 on on IN cord-351964-hduv0ur4 42 32 plastic plastic NN cord-351964-hduv0ur4 42 33 surfaces surface NNS cord-351964-hduv0ur4 42 34 . . . cord-351964-hduv0ur4 43 1 Only only RB cord-351964-hduv0ur4 43 2 between between IN cord-351964-hduv0ur4 43 3 5 5 CD cord-351964-hduv0ur4 43 4 and and CC cord-351964-hduv0ur4 43 5 10 10 CD cord-351964-hduv0ur4 43 6 % % NN cord-351964-hduv0ur4 43 7 of of IN cord-351964-hduv0ur4 43 8 the the DT cord-351964-hduv0ur4 43 9 cells cell NNS cord-351964-hduv0ur4 43 10 on on IN cord-351964-hduv0ur4 43 11 plastic plastic NN cord-351964-hduv0ur4 43 12 can can MD cord-351964-hduv0ur4 43 13 be be VB cord-351964-hduv0ur4 43 14 infected infect VBN cord-351964-hduv0ur4 43 15 , , , cord-351964-hduv0ur4 43 16 whereas whereas IN cord-351964-hduv0ur4 43 17 25 25 CD cord-351964-hduv0ur4 43 18 - - SYM cord-351964-hduv0ur4 43 19 50 50 CD cord-351964-hduv0ur4 43 20 % % NN cord-351964-hduv0ur4 43 21 of of IN cord-351964-hduv0ur4 43 22 the the DT cord-351964-hduv0ur4 43 23 cells cell NNS cord-351964-hduv0ur4 43 24 on on IN cord-351964-hduv0ur4 43 25 ECM ECM NNP cord-351964-hduv0ur4 43 26 are be VBP cord-351964-hduv0ur4 43 27 infected infect VBN cord-351964-hduv0ur4 43 28 . . . cord-351964-hduv0ur4 44 1 This this DT cord-351964-hduv0ur4 44 2 effect effect NN cord-351964-hduv0ur4 44 3 is be VBZ cord-351964-hduv0ur4 44 4 specific specific JJ cord-351964-hduv0ur4 44 5 for for IN cord-351964-hduv0ur4 44 6 MHV MHV NNP cord-351964-hduv0ur4 44 7 - - HYPH cord-351964-hduv0ur4 44 8 A59 A59 NNP cord-351964-hduv0ur4 44 9 . . . cord-351964-hduv0ur4 45 1 Vesicular vesicular JJ cord-351964-hduv0ur4 45 2 stomatitis stomatitis NN cord-351964-hduv0ur4 45 3 virus virus NN cord-351964-hduv0ur4 45 4 ( ( -LRB- cord-351964-hduv0ur4 45 5 VSV VSV NNP cord-351964-hduv0ur4 45 6 ) ) -RRB- cord-351964-hduv0ur4 45 7 infects infect VBZ cord-351964-hduv0ur4 45 8 essentially essentially RB cord-351964-hduv0ur4 45 9 all all PDT cord-351964-hduv0ur4 45 10 the the DT cord-351964-hduv0ur4 45 11 ART20 ART20 NNP cord-351964-hduv0ur4 45 12 cells cell NNS cord-351964-hduv0ur4 45 13 in in IN cord-351964-hduv0ur4 45 14 cultures culture NNS cord-351964-hduv0ur4 45 15 growing grow VBG cord-351964-hduv0ur4 45 16 on on IN cord-351964-hduv0ur4 45 17 plastic plastic NN cord-351964-hduv0ur4 45 18 or or CC cord-351964-hduv0ur4 45 19 on on IN cord-351964-hduv0ur4 45 20 ECM ECM NNP cord-351964-hduv0ur4 45 21 . . . cord-351964-hduv0ur4 46 1 We -PRON- PRP cord-351964-hduv0ur4 46 2 assume assume VBP cord-351964-hduv0ur4 46 3 that that IN cord-351964-hduv0ur4 46 4 when when WRB cord-351964-hduv0ur4 46 5 ART20 art20 VBP cord-351964-hduv0ur4 46 6 cells cell NNS cord-351964-hduv0ur4 46 7 grow grow VBP cord-351964-hduv0ur4 46 8 flattened flatten VBN cord-351964-hduv0ur4 46 9 on on IN cord-351964-hduv0ur4 46 10 ECM ECM NNP cord-351964-hduv0ur4 46 11 , , , cord-351964-hduv0ur4 46 12 either either CC cord-351964-hduv0ur4 46 13 the the DT cord-351964-hduv0ur4 46 14 numbers number NNS cord-351964-hduv0ur4 46 15 or or CC cord-351964-hduv0ur4 46 16 the the DT cord-351964-hduv0ur4 46 17 accessibility accessibility NN cord-351964-hduv0ur4 46 18 of of IN cord-351964-hduv0ur4 46 19 the the DT cord-351964-hduv0ur4 46 20 plasma plasma NN cord-351964-hduv0ur4 46 21 membrane membrane NN cord-351964-hduv0ur4 46 22 receptors receptor NNS cord-351964-hduv0ur4 46 23 exploited exploit VBN cord-351964-hduv0ur4 46 24 by by IN cord-351964-hduv0ur4 46 25 the the DT cord-351964-hduv0ur4 46 26 virus virus NN cord-351964-hduv0ur4 46 27 increases increase VBZ cord-351964-hduv0ur4 46 28 . . . cord-351964-hduv0ur4 47 1 But but CC cord-351964-hduv0ur4 47 2 whatever whatever WDT cord-351964-hduv0ur4 47 3 the the DT cord-351964-hduv0ur4 47 4 mechanism mechanism NN cord-351964-hduv0ur4 47 5 the the DT cord-351964-hduv0ur4 47 6 increased increase VBN cord-351964-hduv0ur4 47 7 susceptibility susceptibility NN cord-351964-hduv0ur4 47 8 of of IN cord-351964-hduv0ur4 47 9 the the DT cord-351964-hduv0ur4 47 10 cells cell NNS cord-351964-hduv0ur4 47 11 to to IN cord-351964-hduv0ur4 47 12 infection infection NN cord-351964-hduv0ur4 47 13 facilitated facilitate VBD cord-351964-hduv0ur4 47 14 several several JJ cord-351964-hduv0ur4 47 15 experiments experiment NNS cord-351964-hduv0ur4 47 16 reported report VBN cord-351964-hduv0ur4 47 17 here here RB cord-351964-hduv0ur4 47 18 . . . cord-351964-hduv0ur4 48 1 Coronavirus Coronavirus NNP cord-351964-hduv0ur4 48 2 MHV MHV NNP cord-351964-hduv0ur4 48 3 - - HYPH cord-351964-hduv0ur4 48 4 A59 A59 NNP cord-351964-hduv0ur4 48 5 , , , cord-351964-hduv0ur4 48 6 propagated propagate VBN cord-351964-hduv0ur4 48 7 on on IN cord-351964-hduv0ur4 48 8 sac sac NN cord-351964-hduv0ur4 48 9 - - HYPH cord-351964-hduv0ur4 48 10 cells cell NNS cord-351964-hduv0ur4 48 11 as as IN cord-351964-hduv0ur4 48 12 previously previously RB cord-351964-hduv0ur4 48 13 described describe VBN cord-351964-hduv0ur4 48 14 ( ( -LRB- cord-351964-hduv0ur4 48 15 Tooze Tooze NNP cord-351964-hduv0ur4 48 16 et et NNP cord-351964-hduv0ur4 48 17 aL aL NNP cord-351964-hduv0ur4 48 18 , , , cord-351964-hduv0ur4 48 19 1984 1984 CD cord-351964-hduv0ur4 48 20 ) ) -RRB- cord-351964-hduv0ur4 48 21 , , , cord-351964-hduv0ur4 48 22 was be VBD cord-351964-hduv0ur4 48 23 used use VBN cord-351964-hduv0ur4 48 24 to to TO cord-351964-hduv0ur4 48 25 infect infect VB cord-351964-hduv0ur4 48 26 ART20 ART20 NNP cord-351964-hduv0ur4 48 27 cells cell NNS cord-351964-hduv0ur4 48 28 at at IN cord-351964-hduv0ur4 48 29 a a DT cord-351964-hduv0ur4 48 30 multiplicity multiplicity NN cord-351964-hduv0ur4 48 31 of of IN cord-351964-hduv0ur4 48 32 infection infection NN cord-351964-hduv0ur4 48 33 of of IN cord-351964-hduv0ur4 48 34 102 102 CD cord-351964-hduv0ur4 48 35 - - SYM cord-351964-hduv0ur4 48 36 103 103 CD cord-351964-hduv0ur4 48 37 pfu pfu NN cord-351964-hduv0ur4 48 38 / / SYM cord-351964-hduv0ur4 48 39 cell cell NN cord-351964-hduv0ur4 48 40 . . . cord-351964-hduv0ur4 49 1 The the DT cord-351964-hduv0ur4 49 2 virus virus NN cord-351964-hduv0ur4 49 3 was be VBD cord-351964-hduv0ur4 49 4 applied apply VBN cord-351964-hduv0ur4 49 5 in in IN cord-351964-hduv0ur4 49 6 1 1 CD cord-351964-hduv0ur4 49 7 rrd rrd NN cord-351964-hduv0ur4 49 8 of of IN cord-351964-hduv0ur4 49 9 culture culture NN cord-351964-hduv0ur4 49 10 medium medium NN cord-351964-hduv0ur4 49 11 per per IN cord-351964-hduv0ur4 49 12 35-ram 35-ram CD cord-351964-hduv0ur4 49 13 dish dish NN cord-351964-hduv0ur4 49 14 for for IN cord-351964-hduv0ur4 49 15 1 1 CD cord-351964-hduv0ur4 49 16 - - SYM cord-351964-hduv0ur4 49 17 2 2 CD cord-351964-hduv0ur4 49 18 h h NN cord-351964-hduv0ur4 49 19 at at IN cord-351964-hduv0ur4 49 20 37~ 37~ CD cord-351964-hduv0ur4 49 21 in in IN cord-351964-hduv0ur4 49 22 a a DT cord-351964-hduv0ur4 49 23 5 5 CD cord-351964-hduv0ur4 49 24 % % NN cord-351964-hduv0ur4 49 25 CO2 CO2 NNP cord-351964-hduv0ur4 49 26 incubator incubator NN cord-351964-hduv0ur4 49 27 . . . cord-351964-hduv0ur4 50 1 A a DT cord-351964-hduv0ur4 50 2 stock stock NN cord-351964-hduv0ur4 50 3 of of IN cord-351964-hduv0ur4 50 4 vesicular vesicular JJ cord-351964-hduv0ur4 50 5 stomatitis stomatitis NN cord-351964-hduv0ur4 50 6 virus virus NN cord-351964-hduv0ur4 50 7 ( ( -LRB- cord-351964-hduv0ur4 50 8 Indiana Indiana NNP cord-351964-hduv0ur4 50 9 serotype serotype NN cord-351964-hduv0ur4 50 10 ) ) -RRB- cord-351964-hduv0ur4 50 11 was be VBD cord-351964-hduv0ur4 50 12 grown grow VBN cord-351964-hduv0ur4 50 13 and and CC cord-351964-hduv0ur4 50 14 titered titere VBN cord-351964-hduv0ur4 50 15 on on IN cord-351964-hduv0ur4 50 16 baby baby NN cord-351964-hduv0ur4 50 17 hamster hamster NN cord-351964-hduv0ur4 50 18 kidney kidney NN cord-351964-hduv0ur4 50 19 cells cell NNS cord-351964-hduv0ur4 50 20 ( ( -LRB- cord-351964-hduv0ur4 50 21 BHK-21 BHK-21 NNP cord-351964-hduv0ur4 50 22 ) ) -RRB- cord-351964-hduv0ur4 50 23 as as IN cord-351964-hduv0ur4 50 24 described describe VBN cord-351964-hduv0ur4 50 25 in in IN cord-351964-hduv0ur4 50 26 Fuller Fuller NNP cord-351964-hduv0ur4 50 27 et et NNP cord-351964-hduv0ur4 50 28 al al NNP cord-351964-hduv0ur4 50 29 . . . cord-351964-hduv0ur4 51 1 ( ( -LRB- cord-351964-hduv0ur4 51 2 1984 1984 CD cord-351964-hduv0ur4 51 3 ) ) -RRB- cord-351964-hduv0ur4 51 4 . . . cord-351964-hduv0ur4 52 1 Infection infection NN cord-351964-hduv0ur4 52 2 of of IN cord-351964-hduv0ur4 52 3 ART20 ART20 NNP cord-351964-hduv0ur4 52 4 cells cell NNS cord-351964-hduv0ur4 52 5 on on IN cord-351964-hduv0ur4 52 6 plastic plastic NN cord-351964-hduv0ur4 52 7 or or CC cord-351964-hduv0ur4 52 8 ECM ECM NNP cord-351964-hduv0ur4 52 9 was be VBD cord-351964-hduv0ur4 52 10 performed perform VBN cord-351964-hduv0ur4 52 11 by by IN cord-351964-hduv0ur4 52 12 rinsing rinse VBG cord-351964-hduv0ur4 52 13 the the DT cord-351964-hduv0ur4 52 14 cell cell NN cord-351964-hduv0ur4 52 15 monolayer monolayer NN cord-351964-hduv0ur4 52 16 with with IN cord-351964-hduv0ur4 52 17 Eagle Eagle NNP cord-351964-hduv0ur4 52 18 's 's POS cord-351964-hduv0ur4 52 19 medium medium NN cord-351964-hduv0ur4 52 20 supplemented supplement VBN cord-351964-hduv0ur4 52 21 with with IN cord-351964-hduv0ur4 52 22 0.2 0.2 CD cord-351964-hduv0ur4 52 23 % % NN cord-351964-hduv0ur4 52 24 BSA BSA NNP cord-351964-hduv0ur4 52 25 , , , cord-351964-hduv0ur4 52 26 10 10 CD cord-351964-hduv0ur4 52 27 mM mM NNP cord-351964-hduv0ur4 52 28 Hepes Hepes NNP cord-351964-hduv0ur4 52 29 , , , cord-351964-hduv0ur4 52 30 2 2 CD cord-351964-hduv0ur4 52 31 mM mm CD cord-351964-hduv0ur4 52 32 glutamine glutamine NN cord-351964-hduv0ur4 52 33 , , , cord-351964-hduv0ur4 52 34 100 100 CD cord-351964-hduv0ur4 52 35 tig tig NNP cord-351964-hduv0ur4 52 36 / / SYM cord-351964-hduv0ur4 52 37 ml ml NNP cord-351964-hduv0ur4 52 38 penicillin penicillin NN cord-351964-hduv0ur4 52 39 , , , cord-351964-hduv0ur4 52 40 and and CC cord-351964-hduv0ur4 52 41 100 100 CD cord-351964-hduv0ur4 52 42 gg gg NNP cord-351964-hduv0ur4 52 43 / / , cord-351964-hduv0ur4 52 44 ml ml NN cord-351964-hduv0ur4 52 45 streptomycin streptomycin NNS cord-351964-hduv0ur4 52 46 , , , cord-351964-hduv0ur4 52 47 and and CC cord-351964-hduv0ur4 52 48 then then RB cord-351964-hduv0ur4 52 49 applying apply VBG cord-351964-hduv0ur4 52 50 the the DT cord-351964-hduv0ur4 52 51 virus virus NN cord-351964-hduv0ur4 52 52 at at IN cord-351964-hduv0ur4 52 53 a a DT cord-351964-hduv0ur4 52 54 multiplicity multiplicity NN cord-351964-hduv0ur4 52 55 of of IN cord-351964-hduv0ur4 52 56 infection infection NN cord-351964-hduv0ur4 52 57 of of IN cord-351964-hduv0ur4 52 58 50 50 CD cord-351964-hduv0ur4 52 59 pill/ pill/ NN cord-351964-hduv0ur4 52 60 cell cell NN cord-351964-hduv0ur4 52 61 in in IN cord-351964-hduv0ur4 52 62 a a DT cord-351964-hduv0ur4 52 63 volume volume NN cord-351964-hduv0ur4 52 64 of of IN cord-351964-hduv0ur4 52 65 0.2 0.2 CD cord-351964-hduv0ur4 52 66 ml ml NNS cord-351964-hduv0ur4 52 67 per per IN cord-351964-hduv0ur4 52 68 35-mm 35-mm CD cord-351964-hduv0ur4 52 69 dish dish NN cord-351964-hduv0ur4 52 70 . . . cord-351964-hduv0ur4 53 1 The the DT cord-351964-hduv0ur4 53 2 monolayers monolayer NNS cord-351964-hduv0ur4 53 3 were be VBD cord-351964-hduv0ur4 53 4 returned return VBN cord-351964-hduv0ur4 53 5 to to IN cord-351964-hduv0ur4 53 6 the the DT cord-351964-hduv0ur4 53 7 37~ 37~ CD cord-351964-hduv0ur4 53 8 5 5 CD cord-351964-hduv0ur4 53 9 % % NN cord-351964-hduv0ur4 53 10 CO2 CO2 NNP cord-351964-hduv0ur4 53 11 incubator incubator NN cord-351964-hduv0ur4 53 12 for for IN cord-351964-hduv0ur4 53 13 1 1 CD cord-351964-hduv0ur4 53 14 h h NN cord-351964-hduv0ur4 53 15 , , , cord-351964-hduv0ur4 53 16 rinsed rinse VBN cord-351964-hduv0ur4 53 17 with with IN cord-351964-hduv0ur4 53 18 the the DT cord-351964-hduv0ur4 53 19 supplemented supplement VBN cord-351964-hduv0ur4 53 20 Eagle Eagle NNP cord-351964-hduv0ur4 53 21 's 's POS cord-351964-hduv0ur4 53 22 medium medium NN cord-351964-hduv0ur4 53 23 to to TO cord-351964-hduv0ur4 53 24 remove remove VB cord-351964-hduv0ur4 53 25 unbound unbound JJ cord-351964-hduv0ur4 53 26 virus virus NN cord-351964-hduv0ur4 53 27 , , , cord-351964-hduv0ur4 53 28 and and CC cord-351964-hduv0ur4 53 29 then then RB cord-351964-hduv0ur4 53 30 with with IN cord-351964-hduv0ur4 53 31 growth growth NN cord-351964-hduv0ur4 53 32 medium medium NN cord-351964-hduv0ur4 53 33 . . . cord-351964-hduv0ur4 54 1 The the DT cord-351964-hduv0ur4 54 2 cells cell NNS cord-351964-hduv0ur4 54 3 were be VBD cord-351964-hduv0ur4 54 4 returned return VBN cord-351964-hduv0ur4 54 5 to to IN cord-351964-hduv0ur4 54 6 the the DT cord-351964-hduv0ur4 54 7 incubator incubator NN cord-351964-hduv0ur4 54 8 in in IN cord-351964-hduv0ur4 54 9 growth growth NN cord-351964-hduv0ur4 54 10 medium medium NN cord-351964-hduv0ur4 54 11 for for IN cord-351964-hduv0ur4 54 12 the the DT cord-351964-hduv0ur4 54 13 remainder remainder NN cord-351964-hduv0ur4 54 14 of of IN cord-351964-hduv0ur4 54 15 the the DT cord-351964-hduv0ur4 54 16 incubation incubation NN cord-351964-hduv0ur4 54 17 period period NN cord-351964-hduv0ur4 54 18 . . . cord-351964-hduv0ur4 55 1 Cells cell NNS cord-351964-hduv0ur4 55 2 growing grow VBG cord-351964-hduv0ur4 55 3 on on IN cord-351964-hduv0ur4 55 4 glass glass NN cord-351964-hduv0ur4 55 5 coverslips coverslip NNS cord-351964-hduv0ur4 55 6 coated coat VBN cord-351964-hduv0ur4 55 7 with with IN cord-351964-hduv0ur4 55 8 ECM ECM NNP cord-351964-hduv0ur4 55 9 were be VBD cord-351964-hduv0ur4 55 10 infected infect VBN cord-351964-hduv0ur4 55 11 with with IN cord-351964-hduv0ur4 55 12 MHV MHV NNP cord-351964-hduv0ur4 55 13 - - HYPH cord-351964-hduv0ur4 55 14 A59 A59 NNP cord-351964-hduv0ur4 55 15 and and CC cord-351964-hduv0ur4 55 16 subsequently subsequently RB cord-351964-hduv0ur4 55 17 superinfected superinfect VBD cord-351964-hduv0ur4 55 18 with with IN cord-351964-hduv0ur4 55 19 VSV VSV NNP cord-351964-hduv0ur4 55 20 as as IN cord-351964-hduv0ur4 55 21 detailed detail VBN cord-351964-hduv0ur4 55 22 in in IN cord-351964-hduv0ur4 55 23 Results result NNS cord-351964-hduv0ur4 55 24 . . . cord-351964-hduv0ur4 56 1 The the DT cord-351964-hduv0ur4 56 2 cells cell NNS cord-351964-hduv0ur4 56 3 were be VBD cord-351964-hduv0ur4 56 4 then then RB cord-351964-hduv0ur4 56 5 fixed fix VBN cord-351964-hduv0ur4 56 6 in in IN cord-351964-hduv0ur4 56 7 3 3 CD cord-351964-hduv0ur4 56 8 % % NN cord-351964-hduv0ur4 56 9 paraformaldehyde paraformaldehyde NNP cord-351964-hduv0ur4 56 10 in in IN cord-351964-hduv0ur4 56 11 PBS PBS NNP cord-351964-hduv0ur4 56 12 containing contain VBG cord-351964-hduv0ur4 56 13 I -PRON- PRP cord-351964-hduv0ur4 57 1 mM mM NNP cord-351964-hduv0ur4 57 2 MgC12 MgC12 NNP cord-351964-hduv0ur4 57 3 and and CC cord-351964-hduv0ur4 58 1 I -PRON- PRP cord-351964-hduv0ur4 58 2 mM mM NNP cord-351964-hduv0ur4 58 3 CaCh CaCh NNPS cord-351964-hduv0ur4 58 4 and and CC cord-351964-hduv0ur4 58 5 labeled label VBN cord-351964-hduv0ur4 58 6 for for IN cord-351964-hduv0ur4 58 7 indirect indirect JJ cord-351964-hduv0ur4 58 8 immunofluorescence immunofluorescence NN cord-351964-hduv0ur4 58 9 microscopy microscopy NNS cord-351964-hduv0ur4 58 10 using use VBG cord-351964-hduv0ur4 58 11 a a DT cord-351964-hduv0ur4 58 12 procedure procedure NN cord-351964-hduv0ur4 58 13 based base VBN cord-351964-hduv0ur4 58 14 on on IN cord-351964-hduv0ur4 58 15 that that DT cord-351964-hduv0ur4 58 16 of of IN cord-351964-hduv0ur4 58 17 Ash Ash NNP cord-351964-hduv0ur4 58 18 et et NNP cord-351964-hduv0ur4 58 19 al al NNP cord-351964-hduv0ur4 58 20 . . . cord-351964-hduv0ur4 59 1 ( ( -LRB- cord-351964-hduv0ur4 59 2 1977 1977 CD cord-351964-hduv0ur4 59 3 ) ) -RRB- cord-351964-hduv0ur4 59 4 . . . cord-351964-hduv0ur4 60 1 Briefly briefly RB cord-351964-hduv0ur4 60 2 the the DT cord-351964-hduv0ur4 60 3 fixed fix VBN cord-351964-hduv0ur4 60 4 cells cell NNS cord-351964-hduv0ur4 60 5 were be VBD cord-351964-hduv0ur4 60 6 first first RB cord-351964-hduv0ur4 60 7 incubated incubate VBN cord-351964-hduv0ur4 60 8 with with IN cord-351964-hduv0ur4 60 9 a a DT cord-351964-hduv0ur4 60 10 rabbit rabbit NN cord-351964-hduv0ur4 60 11 antiserum antiserum NN cord-351964-hduv0ur4 60 12 against against IN cord-351964-hduv0ur4 60 13 VSV VSV NNP cord-351964-hduv0ur4 60 14 - - HYPH cord-351964-hduv0ur4 60 15 G G NNP cord-351964-hduv0ur4 60 16 protein protein NN cord-351964-hduv0ur4 60 17 , , , cord-351964-hduv0ur4 60 18 generously generously RB cord-351964-hduv0ur4 60 19 provided provide VBN cord-351964-hduv0ur4 60 20 by by IN cord-351964-hduv0ur4 60 21 Dr. Dr. NNP cord-351964-hduv0ur4 60 22 B. B. NNP cord-351964-hduv0ur4 60 23 Burke Burke NNP cord-351964-hduv0ur4 60 24 ( ( -LRB- cord-351964-hduv0ur4 60 25 European European NNP cord-351964-hduv0ur4 60 26 Molecular Molecular NNP cord-351964-hduv0ur4 60 27 Biological Biological NNP cord-351964-hduv0ur4 60 28 Laboratory Laboratory NNP cord-351964-hduv0ur4 60 29 , , , cord-351964-hduv0ur4 60 30 Heidelberg Heidelberg NNP cord-351964-hduv0ur4 60 31 ) ) -RRB- cord-351964-hduv0ur4 60 32 , , , cord-351964-hduv0ur4 60 33 and and CC cord-351964-hduv0ur4 60 34 then then RB cord-351964-hduv0ur4 60 35 with with IN cord-351964-hduv0ur4 60 36 rhodamine rhodamine NN cord-351964-hduv0ur4 60 37 - - HYPH cord-351964-hduv0ur4 60 38 conjugated conjugate VBN cord-351964-hduv0ur4 60 39 sheep sheep NN cord-351964-hduv0ur4 60 40 anti anti JJ cord-351964-hduv0ur4 60 41 - - JJ cord-351964-hduv0ur4 60 42 rabbit rabbit JJ cord-351964-hduv0ur4 60 43 antibody antibody NN cord-351964-hduv0ur4 60 44 . . . cord-351964-hduv0ur4 61 1 After after IN cord-351964-hduv0ur4 61 2 this this DT cord-351964-hduv0ur4 61 3 labeling labeling NN cord-351964-hduv0ur4 61 4 the the DT cord-351964-hduv0ur4 61 5 cells cell NNS cord-351964-hduv0ur4 61 6 were be VBD cord-351964-hduv0ur4 61 7 permeabilized permeabilize VBN cord-351964-hduv0ur4 61 8 with with IN cord-351964-hduv0ur4 61 9 0.2 0.2 CD cord-351964-hduv0ur4 61 10 % % NN cord-351964-hduv0ur4 61 11 Triton Triton NNP cord-351964-hduv0ur4 61 12 X-100 x-100 RB cord-351964-hduv0ur4 61 13 and and CC cord-351964-hduv0ur4 61 14 then then RB cord-351964-hduv0ur4 61 15 incubated incubate VBD cord-351964-hduv0ur4 61 16 with with IN cord-351964-hduv0ur4 61 17 an an DT cord-351964-hduv0ur4 61 18 affinity affinity NN cord-351964-hduv0ur4 61 19 - - HYPH cord-351964-hduv0ur4 61 20 purified purify VBN cord-351964-hduv0ur4 61 21 mouse mouse NN cord-351964-hduv0ur4 61 22 monoelonai monoelonai NNP cord-351964-hduv0ur4 61 23 antibody antibody NN cord-351964-hduv0ur4 61 24 against against IN cord-351964-hduv0ur4 61 25 the the DT cord-351964-hduv0ur4 61 26 E1 e1 NN cord-351964-hduv0ur4 61 27 glycoprotein glycoprotein NN cord-351964-hduv0ur4 61 28 of of IN cord-351964-hduv0ur4 61 29 MHV MHV NNP cord-351964-hduv0ur4 61 30 - - HYPH cord-351964-hduv0ur4 61 31 A59 A59 NNP cord-351964-hduv0ur4 61 32 followed follow VBD cord-351964-hduv0ur4 61 33 by by IN cord-351964-hduv0ur4 61 34 fluoresceln fluoresceln NN cord-351964-hduv0ur4 61 35 - - HYPH cord-351964-hduv0ur4 61 36 conjugated conjugate VBN cord-351964-hduv0ur4 61 37 sheep sheep NN cord-351964-hduv0ur4 61 38 anti anti JJ cord-351964-hduv0ur4 61 39 - - JJ cord-351964-hduv0ur4 61 40 mouse mouse JJ cord-351964-hduv0ur4 61 41 antiserum antiserum NN cord-351964-hduv0ur4 61 42 . . . cord-351964-hduv0ur4 62 1 All all DT cord-351964-hduv0ur4 62 2 incubations incubation NNS cord-351964-hduv0ur4 62 3 with with IN cord-351964-hduv0ur4 62 4 antibodies antibody NNS cord-351964-hduv0ur4 62 5 were be VBD cord-351964-hduv0ur4 62 6 at at IN cord-351964-hduv0ur4 62 7 room room NN cord-351964-hduv0ur4 62 8 temperature temperature NN cord-351964-hduv0ur4 62 9 for for IN cord-351964-hduv0ur4 62 10 30 30 CD cord-351964-hduv0ur4 62 11 rain rain NN cord-351964-hduv0ur4 62 12 . . . cord-351964-hduv0ur4 63 1 The the DT cord-351964-hduv0ur4 63 2 double double RB cord-351964-hduv0ur4 63 3 - - HYPH cord-351964-hduv0ur4 63 4 labeled label VBN cord-351964-hduv0ur4 63 5 cells cell NNS cord-351964-hduv0ur4 63 6 were be VBD cord-351964-hduv0ur4 63 7 examined examine VBN cord-351964-hduv0ur4 63 8 in in IN cord-351964-hduv0ur4 63 9 a a DT cord-351964-hduv0ur4 63 10 Zeiss Zeiss NNP cord-351964-hduv0ur4 63 11 photomicroscope photomicroscope NN cord-351964-hduv0ur4 63 12 HI HI NNP cord-351964-hduv0ur4 63 13 with with IN cord-351964-hduv0ur4 63 14 a a DT cord-351964-hduv0ur4 63 15 planapo planapo NNS cord-351964-hduv0ur4 63 16 x63 x63 CD cord-351964-hduv0ur4 63 17 oil oil NN cord-351964-hduv0ur4 63 18 immersion immersion NN cord-351964-hduv0ur4 63 19 objective objective NN cord-351964-hduv0ur4 63 20 and and CC cord-351964-hduv0ur4 63 21 appropriate appropriate JJ cord-351964-hduv0ur4 63 22 filters filter NNS cord-351964-hduv0ur4 63 23 . . . cord-351964-hduv0ur4 64 1 Cells cell NNS cord-351964-hduv0ur4 64 2 grown grow VBN cord-351964-hduv0ur4 64 3 on on IN cord-351964-hduv0ur4 64 4 plastic plastic JJ cord-351964-hduv0ur4 64 5 petri petri JJ cord-351964-hduv0ur4 64 6 dishes dish NNS cord-351964-hduv0ur4 64 7 were be VBD cord-351964-hduv0ur4 64 8 fixed fix VBN cord-351964-hduv0ur4 64 9 for for IN cord-351964-hduv0ur4 64 10 1 1 CD cord-351964-hduv0ur4 64 11 h h NN cord-351964-hduv0ur4 64 12 with with IN cord-351964-hduv0ur4 64 13 modified modify VBN cord-351964-hduv0ur4 64 14 Karnowsky Karnowsky NNP cord-351964-hduv0ur4 64 15 's 's POS cord-351964-hduv0ur4 64 16 fixative fixative NN cord-351964-hduv0ur4 64 17 containing contain VBG cord-351964-hduv0ur4 64 18 2 2 CD cord-351964-hduv0ur4 64 19 % % NN cord-351964-hduv0ur4 64 20 glutaraldehyde glutaraldehyde NN cord-351964-hduv0ur4 64 21 . . . cord-351964-hduv0ur4 65 1 Fixed fix VBN cord-351964-hduv0ur4 65 2 cultures culture NNS cord-351964-hduv0ur4 65 3 were be VBD cord-351964-hduv0ur4 65 4 postfixed postfixe VBN cord-351964-hduv0ur4 65 5 in in IN cord-351964-hduv0ur4 65 6 2 2 CD cord-351964-hduv0ur4 65 7 % % NN cord-351964-hduv0ur4 65 8 osmium osmium NN cord-351964-hduv0ur4 65 9 tetroxide tetroxide NN cord-351964-hduv0ur4 65 10 in in IN cord-351964-hduv0ur4 65 11 0.1 0.1 CD cord-351964-hduv0ur4 65 12 M m NN cord-351964-hduv0ur4 65 13 cacndylate cacndylate NN cord-351964-hduv0ur4 65 14 buffer buffer NN cord-351964-hduv0ur4 65 15 pH pH NNP cord-351964-hduv0ur4 65 16 7.9 7.9 CD cord-351964-hduv0ur4 65 17 for for IN cord-351964-hduv0ur4 65 18 I -PRON- PRP cord-351964-hduv0ur4 65 19 h h NN cord-351964-hduv0ur4 66 1 and and CC cord-351964-hduv0ur4 66 2 then then RB cord-351964-hduv0ur4 66 3 dehydrated dehydrate VBN cord-351964-hduv0ur4 66 4 , , , cord-351964-hduv0ur4 66 5 removed remove VBN cord-351964-hduv0ur4 66 6 from from IN cord-351964-hduv0ur4 66 7 the the DT cord-351964-hduv0ur4 66 8 dishes dish NNS cord-351964-hduv0ur4 66 9 with with IN cord-351964-hduv0ur4 66 10 propylene propylene NN cord-351964-hduv0ur4 66 11 oxide oxide NN cord-351964-hduv0ur4 66 12 , , , cord-351964-hduv0ur4 66 13 and and CC cord-351964-hduv0ur4 66 14 embedded embed VBN cord-351964-hduv0ur4 66 15 in in IN cord-351964-hduv0ur4 66 16 Epon Epon NNP cord-351964-hduv0ur4 66 17 . . . cord-351964-hduv0ur4 67 1 Thin thin JJ cord-351964-hduv0ur4 67 2 sections section NNS cord-351964-hduv0ur4 67 3 were be VBD cord-351964-hduv0ur4 67 4 viewed view VBN cord-351964-hduv0ur4 67 5 in in IN cord-351964-hduv0ur4 67 6 PhUips PhUips NNP cord-351964-hduv0ur4 67 7 electron electron NN cord-351964-hduv0ur4 67 8 microscopes microscope NNS cord-351964-hduv0ur4 67 9 after after IN cord-351964-hduv0ur4 67 10 contrasting contrast VBG cord-351964-hduv0ur4 67 11 with with IN cord-351964-hduv0ur4 67 12 uranyl uranyl NN cord-351964-hduv0ur4 67 13 acetate acetate NN cord-351964-hduv0ur4 67 14 and and CC cord-351964-hduv0ur4 67 15 lead lead NN cord-351964-hduv0ur4 67 16 citrate citrate NN cord-351964-hduv0ur4 67 17 . . . cord-351964-hduv0ur4 68 1 We -PRON- PRP cord-351964-hduv0ur4 68 2 followed follow VBD cord-351964-hduv0ur4 68 3 the the DT cord-351964-hduv0ur4 68 4 procedure procedure NN cord-351964-hduv0ur4 68 5 given give VBN cord-351964-hduv0ur4 68 6 by by IN cord-351964-hduv0ur4 68 7 Reggio Reggio NNP cord-351964-hduv0ur4 68 8 et et NNP cord-351964-hduv0ur4 68 9 al al NNP cord-351964-hduv0ur4 68 10 . . . cord-351964-hduv0ur4 69 1 ( ( -LRB- cord-351964-hduv0ur4 69 2 1983 1983 CD cord-351964-hduv0ur4 69 3 ) ) -RRB- cord-351964-hduv0ur4 69 4 . . . cord-351964-hduv0ur4 70 1 The the DT cord-351964-hduv0ur4 70 2 fixative fixative NN cord-351964-hduv0ur4 70 3 used use VBN cord-351964-hduv0ur4 70 4 was be VBD cord-351964-hduv0ur4 70 5 3 3 CD cord-351964-hduv0ur4 70 6 % % NN cord-351964-hduv0ur4 70 7 paraformaldehyde paraformaldehyde NN cord-351964-hduv0ur4 70 8 and and CC cord-351964-hduv0ur4 70 9 0.1 0.1 CD cord-351964-hduv0ur4 70 10 % % NN cord-351964-hduv0ur4 70 11 glutaraldehydr glutaraldehydr NN cord-351964-hduv0ur4 70 12 in in IN cord-351964-hduv0ur4 70 13 0.1 0.1 CD cord-351964-hduv0ur4 70 14 M m NN cord-351964-hduv0ur4 70 15 sodium sodium NN cord-351964-hduv0ur4 70 16 phosphate phosphate NN cord-351964-hduv0ur4 70 17 , , , cord-351964-hduv0ur4 70 18 pH pH NNP cord-351964-hduv0ur4 70 19 7.2 7.2 CD cord-351964-hduv0ur4 70 20 . . . cord-351964-hduv0ur4 71 1 We -PRON- PRP cord-351964-hduv0ur4 71 2 used use VBD cord-351964-hduv0ur4 71 3 an an DT cord-351964-hduv0ur4 71 4 affinity affinity NN cord-351964-hduv0ur4 71 5 - - HYPH cord-351964-hduv0ur4 71 6 purified purify VBN cord-351964-hduv0ur4 71 7 antibody antibody NN cord-351964-hduv0ur4 71 8 against against IN cord-351964-hduv0ur4 71 9 ACI~ ACI~ , cord-351964-hduv0ur4 71 10 kindly kindly RB cord-351964-hduv0ur4 71 11 provided provide VBN cord-351964-hduv0ur4 71 12 by by IN cord-351964-hduv0ur4 71 13 Dr. Dr. NNP cord-351964-hduv0ur4 71 14 R. R. NNP cord-351964-hduv0ur4 71 15 Kelly Kelly NNP cord-351964-hduv0ur4 71 16 ( ( -LRB- cord-351964-hduv0ur4 71 17 University University NNP cord-351964-hduv0ur4 71 18 of of IN cord-351964-hduv0ur4 71 19 California California NNP cord-351964-hduv0ur4 71 20 , , , cord-351964-hduv0ur4 71 21 San San NNP cord-351964-hduv0ur4 71 22 Francisco Francisco NNP cord-351964-hduv0ur4 71 23 ) ) -RRB- cord-351964-hduv0ur4 71 24 and and CC cord-351964-hduv0ur4 71 25 an an DT cord-351964-hduv0ur4 71 26 aflinity aflinity NN cord-351964-hduv0ur4 71 27 - - HYPH cord-351964-hduv0ur4 71 28 purified purify VBN cord-351964-hduv0ur4 71 29 antiserum antiserum NN cord-351964-hduv0ur4 71 30 against against IN cord-351964-hduv0ur4 71 31 the the DT cord-351964-hduv0ur4 71 32 carboxy carboxy JJ cord-351964-hduv0ur4 71 33 - - HYPH cord-351964-hduv0ur4 71 34 terminal terminal NN cord-351964-hduv0ur4 71 35 domain domain NN cord-351964-hduv0ur4 71 36 of of IN cord-351964-hduv0ur4 71 37 the the DT cord-351964-hduv0ur4 71 38 E1 e1 NN cord-351964-hduv0ur4 71 39 glycoprotein glycoprotein NN cord-351964-hduv0ur4 71 40 MHV MHV NNP cord-351964-hduv0ur4 71 41 - - HYPH cord-351964-hduv0ur4 71 42 A59 A59 NNP cord-351964-hduv0ur4 71 43 ( ( -LRB- cord-351964-hduv0ur4 71 44 ' ' '' cord-351964-hduv0ur4 71 45 Iboze Iboze NNP cord-351964-hduv0ur4 71 46 and and CC cord-351964-hduv0ur4 71 47 Stanley Stanley NNP cord-351964-hduv0ur4 71 48 , , , cord-351964-hduv0ur4 71 49 1986 1986 CD cord-351964-hduv0ur4 71 50 ) ) -RRB- cord-351964-hduv0ur4 71 51 . . . cord-351964-hduv0ur4 72 1 For for IN cord-351964-hduv0ur4 72 2 some some DT cord-351964-hduv0ur4 72 3 of of IN cord-351964-hduv0ur4 72 4 the the DT cord-351964-hduv0ur4 72 5 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 72 6 labeling labeling NN cord-351964-hduv0ur4 72 7 experiments experiment NNS cord-351964-hduv0ur4 72 8 the the DT cord-351964-hduv0ur4 72 9 cells cell NNS cord-351964-hduv0ur4 72 10 were be VBD cord-351964-hduv0ur4 72 11 grown grow VBN cord-351964-hduv0ur4 72 12 on on IN cord-351964-hduv0ur4 72 13 plastic plastic JJ cord-351964-hduv0ur4 72 14 dishes dish NNS cord-351964-hduv0ur4 72 15 coated coat VBN cord-351964-hduv0ur4 72 16 with with IN cord-351964-hduv0ur4 72 17 ECM ECM NNP cord-351964-hduv0ur4 72 18 . . . cord-351964-hduv0ur4 73 1 After after IN cord-351964-hduv0ur4 73 2 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 73 3 labeling label VBG cord-351964-hduv0ur4 73 4 the the DT cord-351964-hduv0ur4 73 5 cells cell NNS cord-351964-hduv0ur4 73 6 were be VBD cord-351964-hduv0ur4 73 7 dehydrated dehydrate VBN cord-351964-hduv0ur4 73 8 , , , cord-351964-hduv0ur4 73 9 removed remove VBN cord-351964-hduv0ur4 73 10 from from IN cord-351964-hduv0ur4 73 11 the the DT cord-351964-hduv0ur4 73 12 dishes dish NNS cord-351964-hduv0ur4 73 13 with with IN cord-351964-hduv0ur4 73 14 propylene propylene NN cord-351964-hduv0ur4 73 15 oxide oxide NN cord-351964-hduv0ur4 73 16 , , , cord-351964-hduv0ur4 73 17 embedded embed VBN cord-351964-hduv0ur4 73 18 in in IN cord-351964-hduv0ur4 73 19 Epon Epon NNP cord-351964-hduv0ur4 73 20 , , , cord-351964-hduv0ur4 73 21 and and CC cord-351964-hduv0ur4 73 22 thin thin JJ cord-351964-hduv0ur4 73 23 sectioned section VBN cord-351964-hduv0ur4 73 24 for for IN cord-351964-hduv0ur4 73 25 electron electron NN cord-351964-hduv0ur4 73 26 microscopy microscopy NN cord-351964-hduv0ur4 73 27 . . . cord-351964-hduv0ur4 74 1 At at IN cord-351964-hduv0ur4 74 2 8-h 8-h CD cord-351964-hduv0ur4 74 3 postinfection postinfection NN cord-351964-hduv0ur4 74 4 five five CD cord-351964-hduv0ur4 74 5 35-mm 35-mm CD cord-351964-hduv0ur4 74 6 dishes dish NNS cord-351964-hduv0ur4 74 7 of of IN cord-351964-hduv0ur4 74 8 AtT20 AtT20 NNP cord-351964-hduv0ur4 74 9 cells cell NNS cord-351964-hduv0ur4 74 10 infected infect VBN cord-351964-hduv0ur4 74 11 with with IN cord-351964-hduv0ur4 74 12 MHV MHV NNP cord-351964-hduv0ur4 74 13 - - HYPH cord-351964-hduv0ur4 74 14 A59 A59 NNP cord-351964-hduv0ur4 74 15 were be VBD cord-351964-hduv0ur4 74 16 washed wash VBN cord-351964-hduv0ur4 74 17 three three CD cord-351964-hduv0ur4 74 18 times time NNS cord-351964-hduv0ur4 74 19 with with IN cord-351964-hduv0ur4 74 20 methionine methionine NN cord-351964-hduv0ur4 74 21 - - HYPH cord-351964-hduv0ur4 74 22 free free JJ cord-351964-hduv0ur4 74 23 modified modify VBN cord-351964-hduv0ur4 74 24 Eagle Eagle NNP cord-351964-hduv0ur4 74 25 's 's POS cord-351964-hduv0ur4 74 26 medium medium NN cord-351964-hduv0ur4 74 27 ( ( -LRB- cord-351964-hduv0ur4 74 28 MEM mem NN cord-351964-hduv0ur4 74 29 - - HYPH cord-351964-hduv0ur4 74 30 met meet VBN cord-351964-hduv0ur4 74 31 ) ) -RRB- cord-351964-hduv0ur4 74 32 containing contain VBG cord-351964-hduv0ur4 74 33 2.2 2.2 CD cord-351964-hduv0ur4 74 34 gm gm NNP cord-351964-hduv0ur4 74 35 / / SYM cord-351964-hduv0ur4 74 36 liter liter NN cord-351964-hduv0ur4 74 37 sodium sodium NN cord-351964-hduv0ur4 74 38 bicarbonate bicarbonate NN cord-351964-hduv0ur4 74 39 , , , cord-351964-hduv0ur4 74 40 and and CC cord-351964-hduv0ur4 74 41 labeled label VBN cord-351964-hduv0ur4 74 42 with with IN cord-351964-hduv0ur4 74 43 0.5 0.5 CD cord-351964-hduv0ur4 74 44 I i NN cord-351964-hduv0ur4 74 45 ~ ~ CD cord-351964-hduv0ur4 74 46 Ci Ci NNP cord-351964-hduv0ur4 74 47 / / SYM cord-351964-hduv0ur4 74 48 gl gl NN cord-351964-hduv0ur4 74 49 of of IN cord-351964-hduv0ur4 75 1 [ [ -LRB- cord-351964-hduv0ur4 75 2 35S]methionlne 35s]methionlne CD cord-351964-hduv0ur4 75 3 in in IN cord-351964-hduv0ur4 75 4 MEM MEM NNP cord-351964-hduv0ur4 75 5 - - HYPH cord-351964-hduv0ur4 75 6 met meet VBD cord-351964-hduv0ur4 75 7 containing contain VBG cord-351964-hduv0ur4 75 8 10 10 CD cord-351964-hduv0ur4 75 9 % % NN cord-351964-hduv0ur4 75 10 dialyzed dialyze VBN cord-351964-hduv0ur4 75 11 FCS FCS NNP cord-351964-hduv0ur4 75 12 , , , cord-351964-hduv0ur4 75 13 10 10 CD cord-351964-hduv0ur4 75 14 mM mM NNP cord-351964-hduv0ur4 75 15 Hepes Hepes NNP cord-351964-hduv0ur4 75 16 , , , cord-351964-hduv0ur4 75 17 2 2 CD cord-351964-hduv0ur4 75 18 mM mm CD cord-351964-hduv0ur4 75 19 glutamine glutamine NN cord-351964-hduv0ur4 75 20 , , , cord-351964-hduv0ur4 75 21 100 100 CD cord-351964-hduv0ur4 75 22 gg gg NNP cord-351964-hduv0ur4 75 23 / / SYM cord-351964-hduv0ur4 75 24 ml ml CC cord-351964-hduv0ur4 75 25 penicillin penicillin NN cord-351964-hduv0ur4 75 26 , , , cord-351964-hduv0ur4 75 27 and and CC cord-351964-hduv0ur4 75 28 100 100 CD cord-351964-hduv0ur4 75 29 ~tg ~tg NNS cord-351964-hduv0ur4 75 30 / / SYM cord-351964-hduv0ur4 75 31 ml ml IN cord-351964-hduv0ur4 75 32 streptomycin streptomycin NNS cord-351964-hduv0ur4 75 33 for for IN cord-351964-hduv0ur4 75 34 10 10 CD cord-351964-hduv0ur4 75 35 rain rain NN cord-351964-hduv0ur4 75 36 . . . cord-351964-hduv0ur4 76 1 The the DT cord-351964-hduv0ur4 76 2 incorporation incorporation NN cord-351964-hduv0ur4 76 3 of of IN cord-351964-hduv0ur4 76 4 [ [ -LRB- cord-351964-hduv0ur4 76 5 ~S]methionine ~S]methionine NNP cord-351964-hduv0ur4 76 6 was be VBD cord-351964-hduv0ur4 76 7 stopped stop VBN cord-351964-hduv0ur4 76 8 by by IN cord-351964-hduv0ur4 76 9 adding add VBG cord-351964-hduv0ur4 76 10 2 2 CD cord-351964-hduv0ur4 76 11 ml ml NNS cord-351964-hduv0ur4 76 12 of of IN cord-351964-hduv0ur4 76 13 growth growth NN cord-351964-hduv0ur4 76 14 medium medium NN cord-351964-hduv0ur4 76 15 per per IN cord-351964-hduv0ur4 76 16 dish dish NN cord-351964-hduv0ur4 76 17 supplemented supplement VBN cord-351964-hduv0ur4 76 18 with with IN cord-351964-hduv0ur4 76 19 lO lO NNP cord-351964-hduv0ur4 76 20 times time NNS cord-351964-hduv0ur4 76 21 the the DT cord-351964-hduv0ur4 76 22 normal normal JJ cord-351964-hduv0ur4 76 23 amount amount NN cord-351964-hduv0ur4 76 24 of of IN cord-351964-hduv0ur4 76 25 methionine methionine NN cord-351964-hduv0ur4 76 26 ( ( -LRB- cord-351964-hduv0ur4 76 27 1.5 1.5 CD cord-351964-hduv0ur4 76 28 mg mg NNP cord-351964-hduv0ur4 76 29 / / SYM cord-351964-hduv0ur4 76 30 ml ml NNP cord-351964-hduv0ur4 76 31 ) ) -RRB- cord-351964-hduv0ur4 76 32 for for IN cord-351964-hduv0ur4 76 33 the the DT cord-351964-hduv0ur4 76 34 chase chase NN cord-351964-hduv0ur4 76 35 , , , cord-351964-hduv0ur4 76 36 or or CC cord-351964-hduv0ur4 76 37 by by IN cord-351964-hduv0ur4 76 38 placing place VBG cord-351964-hduv0ur4 76 39 the the DT cord-351964-hduv0ur4 76 40 cells cell NNS cord-351964-hduv0ur4 76 41 on on IN cord-351964-hduv0ur4 76 42 an an DT cord-351964-hduv0ur4 76 43 aluminium aluminium NN cord-351964-hduv0ur4 76 44 slab slab NN cord-351964-hduv0ur4 76 45 in in IN cord-351964-hduv0ur4 76 46 ice ice NN cord-351964-hduv0ur4 76 47 and and CC cord-351964-hduv0ur4 76 48 washing wash VBG cord-351964-hduv0ur4 76 49 them -PRON- PRP cord-351964-hduv0ur4 76 50 three three CD cord-351964-hduv0ur4 76 51 times time NNS cord-351964-hduv0ur4 76 52 with with IN cord-351964-hduv0ur4 76 53 calcium calcium NN cord-351964-hduv0ur4 76 54 and and CC cord-351964-hduv0ur4 76 55 magnesium magnesium NN cord-351964-hduv0ur4 76 56 - - HYPH cord-351964-hduv0ur4 76 57 free free JJ cord-351964-hduv0ur4 76 58 PBS PBS NNP cord-351964-hduv0ur4 76 59 at at IN cord-351964-hduv0ur4 76 60 4~ 4~ CD cord-351964-hduv0ur4 76 61 for for IN cord-351964-hduv0ur4 76 62 the the DT cord-351964-hduv0ur4 76 63 zero zero CD cord-351964-hduv0ur4 76 64 time time NN cord-351964-hduv0ur4 76 65 point point NN cord-351964-hduv0ur4 76 66 . . . cord-351964-hduv0ur4 77 1 Each each DT cord-351964-hduv0ur4 77 2 35-ram 35-ram CD cord-351964-hduv0ur4 77 3 dish dish NN cord-351964-hduv0ur4 77 4 of of IN cord-351964-hduv0ur4 77 5 cells cell NNS cord-351964-hduv0ur4 77 6 was be VBD cord-351964-hduv0ur4 77 7 solubilized solubilize VBN cord-351964-hduv0ur4 77 8 in in IN cord-351964-hduv0ur4 77 9 RIPA RIPA NNP cord-351964-hduv0ur4 77 10 buffer buffer NN cord-351964-hduv0ur4 77 11 ( ( -LRB- cord-351964-hduv0ur4 77 12 1 1 CD cord-351964-hduv0ur4 77 13 % % NN cord-351964-hduv0ur4 77 14 ( ( -LRB- cord-351964-hduv0ur4 77 15 wt wt NN cord-351964-hduv0ur4 77 16 / / SYM cord-351964-hduv0ur4 77 17 vol vol NN cord-351964-hduv0ur4 77 18 ) ) -RRB- cord-351964-hduv0ur4 77 19 TX-100 TX-100 NNP cord-351964-hduv0ur4 77 20 , , , cord-351964-hduv0ur4 77 21 0.1 0.1 CD cord-351964-hduv0ur4 77 22 % % NN cord-351964-hduv0ur4 77 23 ( ( -LRB- cord-351964-hduv0ur4 77 24 wt wt NN cord-351964-hduv0ur4 77 25 / / SYM cord-351964-hduv0ur4 77 26 vol vol NNP cord-351964-hduv0ur4 77 27 ) ) -RRB- cord-351964-hduv0ur4 77 28 SDS SDS NNP cord-351964-hduv0ur4 77 29 , , , cord-351964-hduv0ur4 77 30 0.15 0.15 CD cord-351964-hduv0ur4 77 31 M M NNP cord-351964-hduv0ur4 77 32 NaC1 NaC1 NNP cord-351964-hduv0ur4 77 33 , , , cord-351964-hduv0ur4 77 34 1 1 CD cord-351964-hduv0ur4 77 35 % % NN cord-351964-hduv0ur4 77 36 ( ( -LRB- cord-351964-hduv0ur4 77 37 wt wt NN cord-351964-hduv0ur4 77 38 / / SYM cord-351964-hduv0ur4 77 39 vol vol NN cord-351964-hduv0ur4 77 40 ) ) -RRB- cord-351964-hduv0ur4 78 1 sodium sodium NNP cord-351964-hduv0ur4 78 2 dodecylate dodecylate NNP cord-351964-hduv0ur4 78 3 , , , cord-351964-hduv0ur4 78 4 10 10 CD cord-351964-hduv0ur4 78 5 mM mM NNP cord-351964-hduv0ur4 78 6 Tris Tris NNP cord-351964-hduv0ur4 78 7 , , , cord-351964-hduv0ur4 78 8 pH pH NNP cord-351964-hduv0ur4 78 9 7.6 7.6 CD cord-351964-hduv0ur4 78 10 ) ) -RRB- cord-351964-hduv0ur4 78 11 containing contain VBG cord-351964-hduv0ur4 78 12 protease protease NN cord-351964-hduv0ur4 78 13 inhibitors inhibitor NNS cord-351964-hduv0ur4 78 14 ( ( -LRB- cord-351964-hduv0ur4 78 15 PI pi NN cord-351964-hduv0ur4 78 16 ) ) -RRB- cord-351964-hduv0ur4 79 1 ( ( -LRB- cord-351964-hduv0ur4 79 2 1 1 CD cord-351964-hduv0ur4 79 3 Ig Ig NNP cord-351964-hduv0ur4 79 4 / / SYM cord-351964-hduv0ur4 79 5 ml ml IN cord-351964-hduv0ur4 79 6 leupeptin leupeptin NN cord-351964-hduv0ur4 79 7 in in IN cord-351964-hduv0ur4 79 8 distilled distil VBN cord-351964-hduv0ur4 79 9 H20 H20 NNP cord-351964-hduv0ur4 79 10 , , , cord-351964-hduv0ur4 79 11 1 1 CD cord-351964-hduv0ur4 79 12 ~g ~g NNP cord-351964-hduv0ur4 79 13 / / SYM cord-351964-hduv0ur4 79 14 ml ml NN cord-351964-hduv0ur4 79 15 antipain antipain NNP cord-351964-hduv0ur4 79 16 in in IN cord-351964-hduv0ur4 79 17 distilled distil VBN cord-351964-hduv0ur4 79 18 H20 H20 NNP cord-351964-hduv0ur4 79 19 , , , cord-351964-hduv0ur4 79 20 1 1 CD cord-351964-hduv0ur4 79 21 ~g ~g NNP cord-351964-hduv0ur4 79 22 / / SYM cord-351964-hduv0ur4 79 23 ml ml IN cord-351964-hduv0ur4 79 24 pepstatin pepstatin NN cord-351964-hduv0ur4 79 25 in in IN cord-351964-hduv0ur4 79 26 H20 H20 NNP cord-351964-hduv0ur4 79 27 , , , cord-351964-hduv0ur4 79 28 1 1 CD cord-351964-hduv0ur4 79 29 mM mm CD cord-351964-hduv0ur4 79 30 benzamidine benzamidine NN cord-351964-hduv0ur4 79 31 in in IN cord-351964-hduv0ur4 79 32 dimethylsulfoxide dimethylsulfoxide NN cord-351964-hduv0ur4 79 33 , , , cord-351964-hduv0ur4 79 34 40 40 CD cord-351964-hduv0ur4 79 35 gg gg NNP cord-351964-hduv0ur4 79 36 / / SYM cord-351964-hduv0ur4 79 37 ml ml NN cord-351964-hduv0ur4 79 38 phenylmethylsulfouyl phenylmethylsulfouyl NN cord-351964-hduv0ur4 79 39 fluoride fluoride NN cord-351964-hduv0ur4 79 40 PMSF PMSF NNP cord-351964-hduv0ur4 79 41 in in IN cord-351964-hduv0ur4 79 42 anhydrous anhydrous JJ cord-351964-hduv0ur4 79 43 ethanol ethanol NN cord-351964-hduv0ur4 79 44 , , , cord-351964-hduv0ur4 79 45 and and CC cord-351964-hduv0ur4 79 46 10 10 CD cord-351964-hduv0ur4 79 47 U u NN cord-351964-hduv0ur4 79 48 / / SYM cord-351964-hduv0ur4 79 49 ml ml NN cord-351964-hduv0ur4 79 50 trasylol trasylol NNP cord-351964-hduv0ur4 79 51 ) ) -RRB- cord-351964-hduv0ur4 80 1 at at IN cord-351964-hduv0ur4 80 2 4~ 4~ CD cord-351964-hduv0ur4 80 3 The the DT cord-351964-hduv0ur4 80 4 insoluble insoluble JJ cord-351964-hduv0ur4 80 5 debris debris NN cord-351964-hduv0ur4 80 6 was be VBD cord-351964-hduv0ur4 80 7 pelleted pellete VBN cord-351964-hduv0ur4 80 8 in in IN cord-351964-hduv0ur4 80 9 an an DT cord-351964-hduv0ur4 80 10 Eppendorf Eppendorf NNP cord-351964-hduv0ur4 80 11 centrifuge centrifuge NN cord-351964-hduv0ur4 80 12 ( ( -LRB- cord-351964-hduv0ur4 80 13 Brinkmmm Brinkmmm NNP cord-351964-hduv0ur4 80 14 Instruments Instruments NNPS cord-351964-hduv0ur4 80 15 , , , cord-351964-hduv0ur4 80 16 Inc. Inc. NNP cord-351964-hduv0ur4 80 17 , , , cord-351964-hduv0ur4 80 18 Westhury Westhury NNP cord-351964-hduv0ur4 80 19 , , , cord-351964-hduv0ur4 80 20 NY NY NNP cord-351964-hduv0ur4 80 21 ) ) -RRB- cord-351964-hduv0ur4 80 22 for for IN cord-351964-hduv0ur4 80 23 5 5 CD cord-351964-hduv0ur4 80 24 min min NN cord-351964-hduv0ur4 80 25 at at IN cord-351964-hduv0ur4 80 26 top top JJ cord-351964-hduv0ur4 80 27 speed speed NN cord-351964-hduv0ur4 80 28 at at IN cord-351964-hduv0ur4 80 29 4~ 4~ CD cord-351964-hduv0ur4 81 1 The the DT cord-351964-hduv0ur4 81 2 supernatant supernatant NN cord-351964-hduv0ur4 81 3 was be VBD cord-351964-hduv0ur4 81 4 preprecipitated preprecipitate VBN cord-351964-hduv0ur4 81 5 with with IN cord-351964-hduv0ur4 81 6 an an DT cord-351964-hduv0ur4 81 7 equal equal JJ cord-351964-hduv0ur4 81 8 volume volume NN cord-351964-hduv0ur4 81 9 of of IN cord-351964-hduv0ur4 81 10 washed wash VBN cord-351964-hduv0ur4 81 11 Pansorbin Pansorbin NNP cord-351964-hduv0ur4 81 12 ( ( -LRB- cord-351964-hduv0ur4 81 13 Calbiochem Calbiochem NNP cord-351964-hduv0ur4 81 14 , , , cord-351964-hduv0ur4 81 15 Frankfurt Frankfurt NNP cord-351964-hduv0ur4 81 16 , , , cord-351964-hduv0ur4 81 17 Federal Federal NNP cord-351964-hduv0ur4 81 18 Republic Republic NNP cord-351964-hduv0ur4 81 19 of of IN cord-351964-hduv0ur4 81 20 Germany Germany NNP cord-351964-hduv0ur4 81 21 ) ) -RRB- cord-351964-hduv0ur4 81 22 in in IN cord-351964-hduv0ur4 81 23 RIPA RIPA NNP cord-351964-hduv0ur4 81 24 / / SYM cord-351964-hduv0ur4 81 25 PI PI NNP cord-351964-hduv0ur4 81 26 ( ( -LRB- cord-351964-hduv0ur4 81 27 20 20 CD cord-351964-hduv0ur4 81 28 % % NN cord-351964-hduv0ur4 81 29 wt wt NN cord-351964-hduv0ur4 81 30 / / SYM cord-351964-hduv0ur4 81 31 vol vol NN cord-351964-hduv0ur4 81 32 ) ) -RRB- cord-351964-hduv0ur4 81 33 ( ( -LRB- cord-351964-hduv0ur4 81 34 see see VB cord-351964-hduv0ur4 81 35 below below RB cord-351964-hduv0ur4 81 36 ) ) -RRB- cord-351964-hduv0ur4 81 37 for for IN cord-351964-hduv0ur4 81 38 45 45 CD cord-351964-hduv0ur4 81 39 rain rain NN cord-351964-hduv0ur4 81 40 at at IN cord-351964-hduv0ur4 81 41 room room NN cord-351964-hduv0ur4 81 42 temperature temperature NN cord-351964-hduv0ur4 81 43 . . . cord-351964-hduv0ur4 82 1 The the DT cord-351964-hduv0ur4 82 2 Pansorbin Pansorbin NNP cord-351964-hduv0ur4 82 3 was be VBD cord-351964-hduv0ur4 82 4 pelleted pellete VBN cord-351964-hduv0ur4 82 5 for for IN cord-351964-hduv0ur4 82 6 3 3 CD cord-351964-hduv0ur4 82 7 rain rain NN cord-351964-hduv0ur4 82 8 at at IN cord-351964-hduv0ur4 82 9 room room NN cord-351964-hduv0ur4 82 10 temperature temperature NN cord-351964-hduv0ur4 82 11 at at IN cord-351964-hduv0ur4 82 12 10,000 10,000 CD cord-351964-hduv0ur4 82 13 rpm rpm NN cord-351964-hduv0ur4 82 14 in in IN cord-351964-hduv0ur4 82 15 an an DT cord-351964-hduv0ur4 82 16 Eppendorf Eppendorf NNP cord-351964-hduv0ur4 82 17 centrifuge centrifuge NN cord-351964-hduv0ur4 82 18 , , , cord-351964-hduv0ur4 82 19 and and CC cord-351964-hduv0ur4 82 20 the the DT cord-351964-hduv0ur4 82 21 clarified clarify VBN cord-351964-hduv0ur4 82 22 supernatant supernatant NN cord-351964-hduv0ur4 82 23 was be VBD cord-351964-hduv0ur4 82 24 removed remove VBN cord-351964-hduv0ur4 82 25 . . . cord-351964-hduv0ur4 83 1 To to IN cord-351964-hduv0ur4 83 2 each each DT cord-351964-hduv0ur4 83 3 sample sample NN cord-351964-hduv0ur4 83 4 10 10 CD cord-351964-hduv0ur4 83 5 gg gg NNS cord-351964-hduv0ur4 83 6 of of IN cord-351964-hduv0ur4 83 7 an an DT cord-351964-hduv0ur4 83 8 affinity affinity NN cord-351964-hduv0ur4 83 9 - - HYPH cord-351964-hduv0ur4 83 10 purified purify VBN cord-351964-hduv0ur4 83 11 antibody antibody NN cord-351964-hduv0ur4 83 12 against against IN cord-351964-hduv0ur4 83 13 the the DT cord-351964-hduv0ur4 83 14 El El NNP cord-351964-hduv0ur4 83 15 glycoprotein glycoprotein NN cord-351964-hduv0ur4 83 16 of of IN cord-351964-hduv0ur4 83 17 MHV MHV NNP cord-351964-hduv0ur4 83 18 - - HYPH cord-351964-hduv0ur4 83 19 A59 A59 NNP cord-351964-hduv0ur4 84 1 ( ( -LRB- cord-351964-hduv0ur4 84 2 Tooze Tooze NNP cord-351964-hduv0ur4 84 3 and and CC cord-351964-hduv0ur4 84 4 Stanley Stanley NNP cord-351964-hduv0ur4 84 5 , , , cord-351964-hduv0ur4 84 6 1986 1986 CD cord-351964-hduv0ur4 84 7 ) ) -RRB- cord-351964-hduv0ur4 84 8 was be VBD cord-351964-hduv0ur4 84 9 added add VBN cord-351964-hduv0ur4 84 10 , , , cord-351964-hduv0ur4 84 11 and and CC cord-351964-hduv0ur4 84 12 the the DT cord-351964-hduv0ur4 84 13 mixture mixture NN cord-351964-hduv0ur4 84 14 was be VBD cord-351964-hduv0ur4 84 15 rotated rotate VBN cord-351964-hduv0ur4 84 16 at at IN cord-351964-hduv0ur4 84 17 4~ 4~ CD cord-351964-hduv0ur4 84 18 overnight overnight RB cord-351964-hduv0ur4 84 19 . . . cord-351964-hduv0ur4 85 1 To to IN cord-351964-hduv0ur4 85 2 the the DT cord-351964-hduv0ur4 85 3 immune immune JJ cord-351964-hduv0ur4 85 4 complexes complex NNS cord-351964-hduv0ur4 85 5 , , , cord-351964-hduv0ur4 85 6 250 250 CD cord-351964-hduv0ur4 85 7 gl gl NNS cord-351964-hduv0ur4 85 8 of of IN cord-351964-hduv0ur4 85 9 washed wash VBN cord-351964-hduv0ur4 85 10 Pansorbin Pansorbin NNP cord-351964-hduv0ur4 85 11 in in IN cord-351964-hduv0ur4 85 12 RIPA]PI ripa]pi ADD cord-351964-hduv0ur4 85 13 ( ( -LRB- cord-351964-hduv0ur4 85 14 10 10 CD cord-351964-hduv0ur4 85 15 % % NN cord-351964-hduv0ur4 85 16 wt wt NN cord-351964-hduv0ur4 85 17 / / SYM cord-351964-hduv0ur4 85 18 vol vol NN cord-351964-hduv0ur4 85 19 ) ) -RRB- cord-351964-hduv0ur4 85 20 was be VBD cord-351964-hduv0ur4 85 21 added add VBN cord-351964-hduv0ur4 85 22 and and CC cord-351964-hduv0ur4 85 23 the the DT cord-351964-hduv0ur4 85 24 mixture mixture NN cord-351964-hduv0ur4 85 25 was be VBD cord-351964-hduv0ur4 85 26 incubated incubate VBN cord-351964-hduv0ur4 85 27 at at IN cord-351964-hduv0ur4 85 28 37~ 37~ CD cord-351964-hduv0ur4 85 29 for for IN cord-351964-hduv0ur4 85 30 60 60 CD cord-351964-hduv0ur4 85 31 rain rain NN cord-351964-hduv0ur4 85 32 and and CC cord-351964-hduv0ur4 85 33 pelleted pelleted NN cord-351964-hduv0ur4 85 34 . . . cord-351964-hduv0ur4 86 1 The the DT cord-351964-hduv0ur4 86 2 pellet pellet NN cord-351964-hduv0ur4 86 3 was be VBD cord-351964-hduv0ur4 86 4 then then RB cord-351964-hduv0ur4 86 5 washed wash VBN cord-351964-hduv0ur4 86 6 three three CD cord-351964-hduv0ur4 86 7 times time NNS cord-351964-hduv0ur4 86 8 with with IN cord-351964-hduv0ur4 86 9 RIPA RIPA NNP cord-351964-hduv0ur4 86 10 / / SYM cord-351964-hduv0ur4 86 11 PI PI NNP cord-351964-hduv0ur4 86 12 buffer buffer NN cord-351964-hduv0ur4 86 13 ; ; : cord-351964-hduv0ur4 86 14 once once RB cord-351964-hduv0ur4 86 15 with with IN cord-351964-hduv0ur4 86 16 10 10 CD cord-351964-hduv0ur4 86 17 mM mM NNP cord-351964-hduv0ur4 86 18 Tris Tris NNP cord-351964-hduv0ur4 86 19 - - HYPH cord-351964-hduv0ur4 86 20 HCl HCl NNP cord-351964-hduv0ur4 86 21 , , , cord-351964-hduv0ur4 86 22 pH pH NNP cord-351964-hduv0ur4 86 23 7.4 7.4 CD cord-351964-hduv0ur4 86 24 , , , cord-351964-hduv0ur4 86 25 0.1 0.1 CD cord-351964-hduv0ur4 86 26 % % NN cord-351964-hduv0ur4 86 27 SDS SDS NNP cord-351964-hduv0ur4 86 28 , , , cord-351964-hduv0ur4 86 29 1 1 CD cord-351964-hduv0ur4 86 30 mM mm CD cord-351964-hduv0ur4 86 31 EDTA EDTA NNP cord-351964-hduv0ur4 86 32 plus plus CC cord-351964-hduv0ur4 86 33 PI pi NN cord-351964-hduv0ur4 86 34 ; ; : cord-351964-hduv0ur4 86 35 once once RB cord-351964-hduv0ur4 86 36 with with IN cord-351964-hduv0ur4 86 37 10 10 CD cord-351964-hduv0ur4 86 38 mM mM NNP cord-351964-hduv0ur4 86 39 Tris Tris NNP cord-351964-hduv0ur4 86 40 - - HYPH cord-351964-hduv0ur4 86 41 HCl HCl NNP cord-351964-hduv0ur4 86 42 , , , cord-351964-hduv0ur4 86 43 pH pH NNP cord-351964-hduv0ur4 86 44 7.4 7.4 CD cord-351964-hduv0ur4 86 45 , , , cord-351964-hduv0ur4 86 46 0.01 0.01 CD cord-351964-hduv0ur4 86 47 % % NN cord-351964-hduv0ur4 86 48 SDS SDS NNP cord-351964-hduv0ur4 86 49 , , , cord-351964-hduv0ur4 86 50 1 1 CD cord-351964-hduv0ur4 86 51 mM mm CD cord-351964-hduv0ur4 86 52 EDTA EDTA NNP cord-351964-hduv0ur4 86 53 plus plus CC cord-351964-hduv0ur4 86 54 PI pi NN cord-351964-hduv0ur4 86 55 ; ; : cord-351964-hduv0ur4 86 56 and and CC cord-351964-hduv0ur4 86 57 once once RB cord-351964-hduv0ur4 86 58 with with IN cord-351964-hduv0ur4 86 59 0.5 0.5 CD cord-351964-hduv0ur4 86 60 M M NNP cord-351964-hduv0ur4 86 61 NaCI NaCI NNP cord-351964-hduv0ur4 86 62 , , , cord-351964-hduv0ur4 86 63 10 10 CD cord-351964-hduv0ur4 86 64 mM mM NNP cord-351964-hduv0ur4 86 65 Tris Tris NNP cord-351964-hduv0ur4 86 66 - - HYPH cord-351964-hduv0ur4 86 67 HCl HCl NNP cord-351964-hduv0ur4 86 68 , , , cord-351964-hduv0ur4 86 69 pH pH NNP cord-351964-hduv0ur4 86 70 7.4 7.4 CD cord-351964-hduv0ur4 86 71 , , , cord-351964-hduv0ur4 86 72 1 1 CD cord-351964-hduv0ur4 86 73 mM mm CD cord-351964-hduv0ur4 86 74 EDTA edta NN cord-351964-hduv0ur4 86 75 . . . cord-351964-hduv0ur4 87 1 The the DT cord-351964-hduv0ur4 87 2 final final JJ cord-351964-hduv0ur4 87 3 wash wash NN cord-351964-hduv0ur4 87 4 was be VBD cord-351964-hduv0ur4 87 5 with with IN cord-351964-hduv0ur4 87 6 10 10 CD cord-351964-hduv0ur4 87 7 mM mM NNP cord-351964-hduv0ur4 87 8 Tris Tris NNP cord-351964-hduv0ur4 87 9 - - HYPH cord-351964-hduv0ur4 87 10 HC1 HC1 NNP cord-351964-hduv0ur4 87 11 , , , cord-351964-hduv0ur4 87 12 pH pH NNP cord-351964-hduv0ur4 87 13 7.4 7.4 CD cord-351964-hduv0ur4 87 14 , , , cord-351964-hduv0ur4 87 15 and and CC cord-351964-hduv0ur4 87 16 1 1 CD cord-351964-hduv0ur4 87 17 mM mm CD cord-351964-hduv0ur4 87 18 EDTA edta NN cord-351964-hduv0ur4 87 19 . . . cord-351964-hduv0ur4 88 1 All all DT cord-351964-hduv0ur4 88 2 washes wash NNS cord-351964-hduv0ur4 88 3 were be VBD cord-351964-hduv0ur4 88 4 for for IN cord-351964-hduv0ur4 88 5 1 1 CD cord-351964-hduv0ur4 88 6 min min NN cord-351964-hduv0ur4 88 7 at at IN cord-351964-hduv0ur4 88 8 10,000 10,000 CD cord-351964-hduv0ur4 88 9 rpm rpm NN cord-351964-hduv0ur4 88 10 at at IN cord-351964-hduv0ur4 88 11 room room NN cord-351964-hduv0ur4 88 12 temperature temperature NN cord-351964-hduv0ur4 88 13 . . . cord-351964-hduv0ur4 89 1 The the DT cord-351964-hduv0ur4 89 2 samples sample NNS cord-351964-hduv0ur4 89 3 were be VBD cord-351964-hduv0ur4 89 4 divided divide VBN cord-351964-hduv0ur4 89 5 in in IN cord-351964-hduv0ur4 89 6 two two CD cord-351964-hduv0ur4 89 7 during during IN cord-351964-hduv0ur4 89 8 the the DT cord-351964-hduv0ur4 89 9 final final JJ cord-351964-hduv0ur4 89 10 wash wash NN cord-351964-hduv0ur4 89 11 . . . cord-351964-hduv0ur4 90 1 One one CD cord-351964-hduv0ur4 90 2 - - HYPH cord-351964-hduv0ur4 90 3 half half NN cord-351964-hduv0ur4 90 4 was be VBD cord-351964-hduv0ur4 90 5 then then RB cord-351964-hduv0ur4 90 6 resuspended resuspend VBN cord-351964-hduv0ur4 90 7 in in IN cord-351964-hduv0ur4 90 8 50 50 CD cord-351964-hduv0ur4 90 9 gl gl NNS cord-351964-hduv0ur4 90 10 of of IN cord-351964-hduv0ur4 90 11 sample sample NN cord-351964-hduv0ur4 90 12 buffer buffer NN cord-351964-hduv0ur4 90 13 containing contain VBG cord-351964-hduv0ur4 90 14 0.2 0.2 CD cord-351964-hduv0ur4 90 15 M M NNP cord-351964-hduv0ur4 90 16 Tris Tris NNP cord-351964-hduv0ur4 90 17 - - HYPH cord-351964-hduv0ur4 90 18 HCI HCI NNP cord-351964-hduv0ur4 90 19 pH ph NN cord-351964-hduv0ur4 90 20 6~8 6~8 CD cord-351964-hduv0ur4 90 21 , , , cord-351964-hduv0ur4 91 1 5 5 LS cord-351964-hduv0ur4 91 2 mM mm CD cord-351964-hduv0ur4 91 3 EDTA EDTA NNP cord-351964-hduv0ur4 91 4 , , , cord-351964-hduv0ur4 91 5 1 1 CD cord-351964-hduv0ur4 91 6 M M NNP cord-351964-hduv0ur4 91 7 sucrose sucrose NN cord-351964-hduv0ur4 91 8 , , , cord-351964-hduv0ur4 91 9 4 4 CD cord-351964-hduv0ur4 91 10 % % NN cord-351964-hduv0ur4 91 11 ( ( -LRB- cord-351964-hduv0ur4 91 12 wt wt NN cord-351964-hduv0ur4 91 13 / / SYM cord-351964-hduv0ur4 91 14 vol vol NNP cord-351964-hduv0ur4 91 15 ) ) -RRB- cord-351964-hduv0ur4 91 16 , , , cord-351964-hduv0ur4 91 17 10 10 CD cord-351964-hduv0ur4 91 18 % % NN cord-351964-hduv0ur4 91 19 ( ( -LRB- cord-351964-hduv0ur4 91 20 wt wt NN cord-351964-hduv0ur4 91 21 / / SYM cord-351964-hduv0ur4 91 22 vol vol NNP cord-351964-hduv0ur4 91 23 ) ) -RRB- cord-351964-hduv0ur4 91 24 SDS SDS NNP cord-351964-hduv0ur4 91 25 , , , cord-351964-hduv0ur4 91 26 bromopbenol bromopbenol NNP cord-351964-hduv0ur4 91 27 blue blue NN cord-351964-hduv0ur4 91 28 , , , cord-351964-hduv0ur4 91 29 and and CC cord-351964-hduv0ur4 91 30 10 10 CD cord-351964-hduv0ur4 91 31 mM mm CD cord-351964-hduv0ur4 91 32 dithiothreitol dithiothreitol NN cord-351964-hduv0ur4 91 33 by by IN cord-351964-hduv0ur4 91 34 shaking shake VBG cord-351964-hduv0ur4 91 35 at at IN cord-351964-hduv0ur4 91 36 37~ 37~ CD cord-351964-hduv0ur4 91 37 for for IN cord-351964-hduv0ur4 91 38 15 15 CD cord-351964-hduv0ur4 91 39 min min NN cord-351964-hduv0ur4 91 40 , , , cord-351964-hduv0ur4 91 41 followed follow VBN cord-351964-hduv0ur4 91 42 by by IN cord-351964-hduv0ur4 91 43 freezing freeze VBG cord-351964-hduv0ur4 91 44 at at IN cord-351964-hduv0ur4 91 45 -80~ -80~ . cord-351964-hduv0ur4 92 1 for for IN cord-351964-hduv0ur4 92 2 5 5 CD cord-351964-hduv0ur4 92 3 min min NN cord-351964-hduv0ur4 92 4 , , , cord-351964-hduv0ur4 92 5 followed follow VBN cord-351964-hduv0ur4 92 6 by by IN cord-351964-hduv0ur4 92 7 an an DT cord-351964-hduv0ur4 92 8 additional additional JJ cord-351964-hduv0ur4 92 9 15-min 15-min CD cord-351964-hduv0ur4 92 10 incubation incubation NN cord-351964-hduv0ur4 92 11 at at IN cord-351964-hduv0ur4 92 12 37~ 37~ CD cord-351964-hduv0ur4 92 13 with with IN cord-351964-hduv0ur4 92 14 shaking shake VBG cord-351964-hduv0ur4 92 15 . . . cord-351964-hduv0ur4 93 1 The the DT cord-351964-hduv0ur4 93 2 insoluble insoluble JJ cord-351964-hduv0ur4 93 3 material material NN cord-351964-hduv0ur4 93 4 was be VBD cord-351964-hduv0ur4 93 5 pelleted pellete VBN cord-351964-hduv0ur4 93 6 in in IN cord-351964-hduv0ur4 93 7 an an DT cord-351964-hduv0ur4 93 8 Eppendorf Eppendorf NNP cord-351964-hduv0ur4 93 9 centrifuge centrifuge NN cord-351964-hduv0ur4 93 10 ( ( -LRB- cord-351964-hduv0ur4 93 11 Bdnkmann Bdnkmann NNP cord-351964-hduv0ur4 93 12 Instruments Instruments NNP cord-351964-hduv0ur4 93 13 , , , cord-351964-hduv0ur4 93 14 Inc. Inc. NNP cord-351964-hduv0ur4 93 15 ) ) -RRB- cord-351964-hduv0ur4 93 16 for for IN cord-351964-hduv0ur4 93 17 2 2 CD cord-351964-hduv0ur4 93 18 rain rain NN cord-351964-hduv0ur4 93 19 at at IN cord-351964-hduv0ur4 93 20 top top JJ cord-351964-hduv0ur4 93 21 speed speed NN cord-351964-hduv0ur4 93 22 at at IN cord-351964-hduv0ur4 93 23 room room NN cord-351964-hduv0ur4 93 24 temperature temperature NN cord-351964-hduv0ur4 93 25 , , , cord-351964-hduv0ur4 93 26 and and CC cord-351964-hduv0ur4 93 27 prepared prepare VBD cord-351964-hduv0ur4 93 28 for for IN cord-351964-hduv0ur4 93 29 SDS SDS NNP cord-351964-hduv0ur4 93 30 - - HYPH cord-351964-hduv0ur4 93 31 PAGE PAGE NNP cord-351964-hduv0ur4 93 32 as as IN cord-351964-hduv0ur4 93 33 previously previously RB cord-351964-hduv0ur4 93 34 described describe VBN cord-351964-hduv0ur4 93 35 ( ( -LRB- cord-351964-hduv0ur4 93 36 Green Green NNP cord-351964-hduv0ur4 93 37 et et FW cord-351964-hduv0ur4 93 38 ai ai NNP cord-351964-hduv0ur4 93 39 . . NNP cord-351964-hduv0ur4 93 40 , , , cord-351964-hduv0ur4 93 41 1981 1981 CD cord-351964-hduv0ur4 93 42 ) ) -RRB- cord-351964-hduv0ur4 93 43 . . . cord-351964-hduv0ur4 94 1 The the DT cord-351964-hduv0ur4 94 2 other other JJ cord-351964-hduv0ur4 94 3 half half NN cord-351964-hduv0ur4 94 4 was be VBD cord-351964-hduv0ur4 94 5 washed wash VBN cord-351964-hduv0ur4 94 6 twice twice RB cord-351964-hduv0ur4 94 7 and and CC cord-351964-hduv0ur4 94 8 resuspended resuspend VBD cord-351964-hduv0ur4 94 9 in in IN cord-351964-hduv0ur4 94 10 100 100 CD cord-351964-hduv0ur4 94 11 gl gl NNS cord-351964-hduv0ur4 94 12 of of IN cord-351964-hduv0ur4 94 13 100 100 CD cord-351964-hduv0ur4 94 14 mM mm CD cord-351964-hduv0ur4 94 15 sodium sodium NN cord-351964-hduv0ur4 94 16 acetate acetate NN cord-351964-hduv0ur4 94 17 , , , cord-351964-hduv0ur4 94 18 pH pH NNP cord-351964-hduv0ur4 94 19 5.6 5.6 CD cord-351964-hduv0ur4 94 20 and and CC cord-351964-hduv0ur4 94 21 1 1 CD cord-351964-hduv0ur4 94 22 mM mm CD cord-351964-hduv0ur4 94 23 CaC12 cac12 JJ cord-351964-hduv0ur4 94 24 at at IN cord-351964-hduv0ur4 94 25 4~ 4~ CD cord-351964-hduv0ur4 95 1 Then then RB cord-351964-hduv0ur4 95 2 20 20 CD cord-351964-hduv0ur4 95 3 gl gl NNS cord-351964-hduv0ur4 95 4 of of IN cord-351964-hduv0ur4 95 5 2 2 CD cord-351964-hduv0ur4 95 6 mU mU NNP cord-351964-hduv0ur4 95 7 / / SYM cord-351964-hduv0ur4 95 8 gl gl NNP cord-351964-hduv0ur4 95 9 neuraminidase neuraminidase NN cord-351964-hduv0ur4 95 10 from from IN cord-351964-hduv0ur4 95 11 Clostridium clostridium NN cord-351964-hduv0ur4 95 12 perfringens perfringen NNS cord-351964-hduv0ur4 95 13 ( ( -LRB- cord-351964-hduv0ur4 95 14 Sigma Sigma NNP cord-351964-hduv0ur4 95 15 Chemical Chemical NNP cord-351964-hduv0ur4 95 16 Co. Co. NNP cord-351964-hduv0ur4 95 17 , , , cord-351964-hduv0ur4 95 18 Munich Munich NNP cord-351964-hduv0ur4 95 19 , , , cord-351964-hduv0ur4 95 20 FRG FRG NNP cord-351964-hduv0ur4 95 21 ) ) -RRB- cord-351964-hduv0ur4 95 22 in in IN cord-351964-hduv0ur4 95 23 100 100 CD cord-351964-hduv0ur4 95 24 mM mm CD cord-351964-hduv0ur4 95 25 sodium sodium NN cord-351964-hduv0ur4 95 26 acetate acetate NN cord-351964-hduv0ur4 95 27 , , , cord-351964-hduv0ur4 96 1 pH pH NNP cord-351964-hduv0ur4 96 2 7.6 7.6 CD cord-351964-hduv0ur4 96 3 , , , cord-351964-hduv0ur4 96 4 1 1 CD cord-351964-hduv0ur4 96 5 mM mm CD cord-351964-hduv0ur4 96 6 CaCI2 CaCI2 NNP cord-351964-hduv0ur4 96 7 was be VBD cord-351964-hduv0ur4 96 8 added add VBN cord-351964-hduv0ur4 96 9 , , , cord-351964-hduv0ur4 96 10 and and CC cord-351964-hduv0ur4 96 11 the the DT cord-351964-hduv0ur4 96 12 volume volume NN cord-351964-hduv0ur4 96 13 was be VBD cord-351964-hduv0ur4 96 14 adjusted adjust VBN cord-351964-hduv0ur4 96 15 to to IN cord-351964-hduv0ur4 96 16 200 200 CD cord-351964-hduv0ur4 96 17 I i NN cord-351964-hduv0ur4 97 1 ~l ~l NNP cord-351964-hduv0ur4 97 2 with with IN cord-351964-hduv0ur4 97 3 the the DT cord-351964-hduv0ur4 97 4 same same JJ cord-351964-hduv0ur4 97 5 buffer buffer NN cord-351964-hduv0ur4 97 6 . . . cord-351964-hduv0ur4 98 1 After after IN cord-351964-hduv0ur4 98 2 a a DT cord-351964-hduv0ur4 98 3 16-h 16-h CD cord-351964-hduv0ur4 98 4 incubation incubation NN cord-351964-hduv0ur4 98 5 rotating rotate VBG cord-351964-hduv0ur4 98 6 end end NN cord-351964-hduv0ur4 98 7 over over IN cord-351964-hduv0ur4 98 8 end end NN cord-351964-hduv0ur4 98 9 at at IN cord-351964-hduv0ur4 98 10 37~ 37~ CD cord-351964-hduv0ur4 99 1 the the DT cord-351964-hduv0ur4 99 2 Pansorbin Pansorbin NNP cord-351964-hduv0ur4 99 3 was be VBD cord-351964-hduv0ur4 99 4 pelleted pellete VBN cord-351964-hduv0ur4 99 5 , , , cord-351964-hduv0ur4 99 6 solubilized solubilize VBN cord-351964-hduv0ur4 99 7 in in IN cord-351964-hduv0ur4 99 8 sample sample NN cord-351964-hduv0ur4 99 9 buffer buffer NN cord-351964-hduv0ur4 99 10 to to TO cord-351964-hduv0ur4 99 11 release release VB cord-351964-hduv0ur4 99 12 the the DT cord-351964-hduv0ur4 99 13 digested digest VBN cord-351964-hduv0ur4 99 14 E1 e1 NN cord-351964-hduv0ur4 99 15 glycoprotein glycoprotein NN cord-351964-hduv0ur4 99 16 , , , cord-351964-hduv0ur4 99 17 and and CC cord-351964-hduv0ur4 99 18 run run VB cord-351964-hduv0ur4 99 19 on on IN cord-351964-hduv0ur4 99 20 a a DT cord-351964-hduv0ur4 99 21 15 15 CD cord-351964-hduv0ur4 99 22 % % NN cord-351964-hduv0ur4 99 23 SDS sds NN cord-351964-hduv0ur4 99 24 - - HYPH cord-351964-hduv0ur4 99 25 polyacrylamide polyacrylamide NN cord-351964-hduv0ur4 99 26 gel gel NN cord-351964-hduv0ur4 99 27 . . . cord-351964-hduv0ur4 100 1 The the DT cord-351964-hduv0ur4 100 2 pulse pulse NN cord-351964-hduv0ur4 100 3 chase chase NN cord-351964-hduv0ur4 100 4 to to TO cord-351964-hduv0ur4 100 5 evaluate evaluate VB cord-351964-hduv0ur4 100 6 the the DT cord-351964-hduv0ur4 100 7 extent extent NN cord-351964-hduv0ur4 100 8 of of IN cord-351964-hduv0ur4 100 9 N n CD cord-351964-hduv0ur4 100 10 - - HYPH cord-351964-hduv0ur4 100 11 glycosylatiou glycosylatiou NN cord-351964-hduv0ur4 100 12 of of IN cord-351964-hduv0ur4 100 13 vesicular vesicular JJ cord-351964-hduv0ur4 100 14 stomatitis stomatitis NN cord-351964-hduv0ur4 100 15 G g NN cord-351964-hduv0ur4 100 16 protein protein NN cord-351964-hduv0ur4 100 17 in in IN cord-351964-hduv0ur4 100 18 cells cell NNS cord-351964-hduv0ur4 100 19 infected infect VBN cord-351964-hduv0ur4 100 20 with with IN cord-351964-hduv0ur4 100 21 the the DT cord-351964-hduv0ur4 100 22 corouavirus corouavirus JJ cord-351964-hduv0ur4 100 23 MHV MHV NNP cord-351964-hduv0ur4 100 24 - - HYPH cord-351964-hduv0ur4 100 25 A59 A59 NNP cord-351964-hduv0ur4 100 26 was be VBD cord-351964-hduv0ur4 100 27 performed perform VBN cord-351964-hduv0ur4 100 28 essentially essentially RB cord-351964-hduv0ur4 100 29 as as IN cord-351964-hduv0ur4 100 30 described describe VBN cord-351964-hduv0ur4 100 31 by by IN cord-351964-hduv0ur4 100 32 Fuller Fuller NNP cord-351964-hduv0ur4 100 33 et et NNP cord-351964-hduv0ur4 100 34 al al NNP cord-351964-hduv0ur4 100 35 . . . cord-351964-hduv0ur4 101 1 ( ( -LRB- cord-351964-hduv0ur4 101 2 1985 1985 CD cord-351964-hduv0ur4 101 3 ) ) -RRB- cord-351964-hduv0ur4 101 4 . . . cord-351964-hduv0ur4 102 1 Infected infected JJ cord-351964-hduv0ur4 102 2 cells cell NNS cord-351964-hduv0ur4 102 3 were be VBD cord-351964-hduv0ur4 102 4 pulsed pulse VBN cord-351964-hduv0ur4 102 5 with with IN cord-351964-hduv0ur4 102 6 [ [ -LRB- cord-351964-hduv0ur4 102 7 ~SS]methionine ~ss]methionine NN cord-351964-hduv0ur4 102 8 ( ( -LRB- cord-351964-hduv0ur4 102 9 100 100 CD cord-351964-hduv0ur4 102 10 I i NN cord-351964-hduv0ur4 102 11 ~ ~ CD cord-351964-hduv0ur4 102 12 Ci Ci NNP cord-351964-hduv0ur4 102 13 in in IN cord-351964-hduv0ur4 102 14 0.5 0.5 CD cord-351964-hduv0ur4 102 15 ml ml NNS cord-351964-hduv0ur4 102 16 of of IN cord-351964-hduv0ur4 102 17 MEM MEM NNP cord-351964-hduv0ur4 102 18 lacking lack VBG cord-351964-hduv0ur4 102 19 methionine methionine NN cord-351964-hduv0ur4 102 20 and and CC cord-351964-hduv0ur4 102 21 supplemented supplement VBN cord-351964-hduv0ur4 102 22 with with IN cord-351964-hduv0ur4 102 23 0.2 0.2 CD cord-351964-hduv0ur4 102 24 % % NN cord-351964-hduv0ur4 102 25 BSA bsa NN cord-351964-hduv0ur4 102 26 per per IN cord-351964-hduv0ur4 102 27 25-ram 25-ram CD cord-351964-hduv0ur4 102 28 dish dish NN cord-351964-hduv0ur4 102 29 ) ) -RRB- cord-351964-hduv0ur4 102 30 for for IN cord-351964-hduv0ur4 102 31 10 10 CD cord-351964-hduv0ur4 102 32 min min NN cord-351964-hduv0ur4 102 33 and and CC cord-351964-hduv0ur4 102 34 then then RB cord-351964-hduv0ur4 102 35 chased chase VBD cord-351964-hduv0ur4 102 36 with with IN cord-351964-hduv0ur4 102 37 3 3 CD cord-351964-hduv0ur4 102 38 ml ml NNS cord-351964-hduv0ur4 102 39 of of IN cord-351964-hduv0ur4 102 40 the the DT cord-351964-hduv0ur4 102 41 same same JJ cord-351964-hduv0ur4 102 42 medium medium NN cord-351964-hduv0ur4 102 43 containing contain VBG cord-351964-hduv0ur4 102 44 1.5 1.5 CD cord-351964-hduv0ur4 102 45 mg mg NNP cord-351964-hduv0ur4 102 46 / / SYM cord-351964-hduv0ur4 102 47 ml ml NN cord-351964-hduv0ur4 102 48 methionine methionine NN cord-351964-hduv0ur4 102 49 for for IN cord-351964-hduv0ur4 102 50 various various JJ cord-351964-hduv0ur4 102 51 times time NNS cord-351964-hduv0ur4 102 52 . . . cord-351964-hduv0ur4 103 1 The the DT cord-351964-hduv0ur4 103 2 cells cell NNS cord-351964-hduv0ur4 103 3 were be VBD cord-351964-hduv0ur4 103 4 harvested harvest VBN cord-351964-hduv0ur4 103 5 by by IN cord-351964-hduv0ur4 103 6 cooling cool VBG cord-351964-hduv0ur4 103 7 on on IN cord-351964-hduv0ur4 103 8 ice ice NN cord-351964-hduv0ur4 103 9 , , , cord-351964-hduv0ur4 103 10 rinsing rinse VBG cord-351964-hduv0ur4 103 11 twice twice RB cord-351964-hduv0ur4 103 12 with with IN cord-351964-hduv0ur4 103 13 ice ice NN cord-351964-hduv0ur4 103 14 - - HYPH cord-351964-hduv0ur4 103 15 cold cold JJ cord-351964-hduv0ur4 103 16 PBS PBS NNP cord-351964-hduv0ur4 103 17 , , , cord-351964-hduv0ur4 103 18 and and CC cord-351964-hduv0ur4 103 19 then then RB cord-351964-hduv0ur4 103 20 lysed lyse VBN cord-351964-hduv0ur4 103 21 with with IN cord-351964-hduv0ur4 103 22 0.5 0.5 CD cord-351964-hduv0ur4 103 23 ml ml NNS cord-351964-hduv0ur4 103 24 of of IN cord-351964-hduv0ur4 103 25 2 2 CD cord-351964-hduv0ur4 103 26 % % NN cord-351964-hduv0ur4 103 27 Triton Triton NNP cord-351964-hduv0ur4 103 28 X X NNP cord-351964-hduv0ur4 103 29 - - NNP cord-351964-hduv0ur4 103 30 I14 I14 NNP cord-351964-hduv0ur4 103 31 in in IN cord-351964-hduv0ur4 103 32 PBS PBS NNP cord-351964-hduv0ur4 103 33 cor cor NNP cord-351964-hduv0ur4 103 34 ~ ~ NFP cord-351964-hduv0ur4 103 35 nining nining NN cord-351964-hduv0ur4 103 36 1 1 CD cord-351964-hduv0ur4 103 37 mM mm CD cord-351964-hduv0ur4 103 38 phenylmethylsulfouyl phenylmethylsulfouyl NN cord-351964-hduv0ur4 103 39 fluoride fluoride NN cord-351964-hduv0ur4 103 40 . . . cord-351964-hduv0ur4 104 1 The the DT cord-351964-hduv0ur4 104 2 cells cell NNS cord-351964-hduv0ur4 104 3 were be VBD cord-351964-hduv0ur4 104 4 scraped scrape VBN cord-351964-hduv0ur4 104 5 from from IN cord-351964-hduv0ur4 104 6 the the DT cord-351964-hduv0ur4 104 7 dish dish NN cord-351964-hduv0ur4 104 8 with with IN cord-351964-hduv0ur4 104 9 a a DT cord-351964-hduv0ur4 104 10 plastic plastic NN cord-351964-hduv0ur4 104 11 pipette pipette NN cord-351964-hduv0ur4 104 12 tip tip NN cord-351964-hduv0ur4 104 13 and and CC cord-351964-hduv0ur4 104 14 the the DT cord-351964-hduv0ur4 104 15 lysate lysate NN cord-351964-hduv0ur4 104 16 spun spin VBD cord-351964-hduv0ur4 104 17 at at IN cord-351964-hduv0ur4 104 18 600 600 CD cord-351964-hduv0ur4 104 19 g g NN cord-351964-hduv0ur4 104 20 for for IN cord-351964-hduv0ur4 104 21 5 5 CD cord-351964-hduv0ur4 104 22 rain rain NN cord-351964-hduv0ur4 104 23 at at IN cord-351964-hduv0ur4 104 24 4~ 4~ CD cord-351964-hduv0ur4 104 25 to to TO cord-351964-hduv0ur4 104 26 remove remove VB cord-351964-hduv0ur4 104 27 nuclei nucleus NNS cord-351964-hduv0ur4 104 28 . . . cord-351964-hduv0ur4 105 1 The the DT cord-351964-hduv0ur4 105 2 supernatant supernatant NN cord-351964-hduv0ur4 105 3 was be VBD cord-351964-hduv0ur4 105 4 then then RB cord-351964-hduv0ur4 105 5 subjected subject VBN cord-351964-hduv0ur4 105 6 to to IN cord-351964-hduv0ur4 105 7 two two CD cord-351964-hduv0ur4 105 8 rounds round NNS cord-351964-hduv0ur4 105 9 of of IN cord-351964-hduv0ur4 105 10 phase phase NN cord-351964-hduv0ur4 105 11 partitioning partition VBG cord-351964-hduv0ur4 105 12 Bordier Bordier NNP cord-351964-hduv0ur4 105 13 , , , cord-351964-hduv0ur4 105 14 1981 1981 CD cord-351964-hduv0ur4 105 15 ) ) -RRB- cord-351964-hduv0ur4 105 16 to to TO cord-351964-hduv0ur4 105 17 enrich enrich VB cord-351964-hduv0ur4 105 18 for for IN cord-351964-hduv0ur4 105 19 the the DT cord-351964-hduv0ur4 105 20 G g NN cord-351964-hduv0ur4 105 21 protein protein NN cord-351964-hduv0ur4 105 22 . . . cord-351964-hduv0ur4 106 1 Equal equal JJ cord-351964-hduv0ur4 106 2 numbers number NNS cord-351964-hduv0ur4 106 3 of of IN cord-351964-hduv0ur4 106 4 counts count NNS cord-351964-hduv0ur4 106 5 ( ( -LRB- cord-351964-hduv0ur4 106 6 corresponding correspond VBG cord-351964-hduv0ur4 106 7 to to IN cord-351964-hduv0ur4 106 8 approximately approximately RB cord-351964-hduv0ur4 106 9 one one CD cord-351964-hduv0ur4 106 10 - - HYPH cord-351964-hduv0ur4 106 11 twentieth twentieth NN cord-351964-hduv0ur4 106 12 of of IN cord-351964-hduv0ur4 106 13 the the DT cord-351964-hduv0ur4 106 14 total total JJ cord-351964-hduv0ur4 106 15 lysate lysate NN cord-351964-hduv0ur4 106 16 ) ) -RRB- cord-351964-hduv0ur4 106 17 were be VBD cord-351964-hduv0ur4 106 18 then then RB cord-351964-hduv0ur4 106 19 prepared prepare VBN cord-351964-hduv0ur4 106 20 for for IN cord-351964-hduv0ur4 106 21 SDS SDS NNP cord-351964-hduv0ur4 106 22 - - HYPH cord-351964-hduv0ur4 106 23 PAGE PAGE NNP cord-351964-hduv0ur4 106 24 on on IN cord-351964-hduv0ur4 106 25 a a DT cord-351964-hduv0ur4 106 26 10 10 CD cord-351964-hduv0ur4 106 27 - - SYM cord-351964-hduv0ur4 106 28 15 15 CD cord-351964-hduv0ur4 106 29 % % NN cord-351964-hduv0ur4 106 30 polyacrylamide polyacrylamide NN cord-351964-hduv0ur4 106 31 gel gel NN cord-351964-hduv0ur4 106 32 . . . cord-351964-hduv0ur4 107 1 Replication replication NN cord-351964-hduv0ur4 107 2 of of IN cord-351964-hduv0ur4 107 3 MHV MHV NNP cord-351964-hduv0ur4 107 4 - - HYPH cord-351964-hduv0ur4 107 5 A59 A59 NNP cord-351964-hduv0ur4 107 6 in in IN cord-351964-hduv0ur4 107 7 AtT20 AtT20 NNP cord-351964-hduv0ur4 107 8 cells cell NNS cord-351964-hduv0ur4 107 9 ultimately ultimately RB cord-351964-hduv0ur4 107 10 results result VBZ cord-351964-hduv0ur4 107 11 in in IN cord-351964-hduv0ur4 107 12 disruption disruption NN cord-351964-hduv0ur4 107 13 of of IN cord-351964-hduv0ur4 107 14 the the DT cord-351964-hduv0ur4 107 15 Golgi Golgi NNP cord-351964-hduv0ur4 107 16 apparatus apparatus NN cord-351964-hduv0ur4 107 17 and and CC cord-351964-hduv0ur4 107 18 death death NN cord-351964-hduv0ur4 107 19 of of IN cord-351964-hduv0ur4 107 20 the the DT cord-351964-hduv0ur4 107 21 cells cell NNS cord-351964-hduv0ur4 107 22 . . . cord-351964-hduv0ur4 108 1 It -PRON- PRP cord-351964-hduv0ur4 108 2 was be VBD cord-351964-hduv0ur4 108 3 necessary necessary JJ cord-351964-hduv0ur4 108 4 , , , cord-351964-hduv0ur4 108 5 therefore therefore RB cord-351964-hduv0ur4 108 6 , , , cord-351964-hduv0ur4 108 7 to to TO cord-351964-hduv0ur4 108 8 establish establish VB cord-351964-hduv0ur4 108 9 that that DT cord-351964-hduv0ur4 108 10 at at IN cord-351964-hduv0ur4 108 11 early early JJ cord-351964-hduv0ur4 108 12 times time NNS cord-351964-hduv0ur4 108 13 postinfection postinfection NN cord-351964-hduv0ur4 108 14 the the DT cord-351964-hduv0ur4 108 15 Golgi Golgi NNP cord-351964-hduv0ur4 108 16 apparatus apparatus NN cord-351964-hduv0ur4 108 17 maintains maintain VBZ cord-351964-hduv0ur4 108 18 its -PRON- PRP$ cord-351964-hduv0ur4 108 19 structure structure NN cord-351964-hduv0ur4 108 20 and and CC cord-351964-hduv0ur4 108 21 function function NN cord-351964-hduv0ur4 108 22 and and CC cord-351964-hduv0ur4 108 23 is be VBZ cord-351964-hduv0ur4 108 24 not not RB cord-351964-hduv0ur4 108 25 yet yet RB cord-351964-hduv0ur4 108 26 disrupted disrupt VBN cord-351964-hduv0ur4 108 27 by by IN cord-351964-hduv0ur4 108 28 the the DT cord-351964-hduv0ur4 108 29 cytopathic cytopathic JJ cord-351964-hduv0ur4 108 30 effects effect NNS cord-351964-hduv0ur4 108 31 of of IN cord-351964-hduv0ur4 108 32 viral viral JJ cord-351964-hduv0ur4 108 33 replication replication NN cord-351964-hduv0ur4 108 34 . . . cord-351964-hduv0ur4 109 1 Until until IN cord-351964-hduv0ur4 109 2 at at RB cord-351964-hduv0ur4 109 3 least least JJS cord-351964-hduv0ur4 109 4 10 10 CD cord-351964-hduv0ur4 109 5 h h NN cord-351964-hduv0ur4 109 6 after after IN cord-351964-hduv0ur4 109 7 infection infection NN cord-351964-hduv0ur4 109 8 of of IN cord-351964-hduv0ur4 109 9 AtT20 AtT20 NNP cord-351964-hduv0ur4 109 10 cells cell NNS cord-351964-hduv0ur4 109 11 with with IN cord-351964-hduv0ur4 109 12 MHV MHV NNP cord-351964-hduv0ur4 109 13 - - HYPH cord-351964-hduv0ur4 109 14 A59 A59 NNP cord-351964-hduv0ur4 109 15 the the DT cord-351964-hduv0ur4 109 16 Golgi Golgi NNP cord-351964-hduv0ur4 109 17 apparatus apparatus NN cord-351964-hduv0ur4 109 18 maintains maintain VBZ cord-351964-hduv0ur4 109 19 its -PRON- PRP$ cord-351964-hduv0ur4 109 20 stacked stack VBN cord-351964-hduv0ur4 109 21 cistemal cistemal JJ cord-351964-hduv0ur4 109 22 structure structure NN cord-351964-hduv0ur4 109 23 . . . cord-351964-hduv0ur4 110 1 Progeny progeny JJ cord-351964-hduv0ur4 110 2 virus virus NN cord-351964-hduv0ur4 110 3 particles particle NNS cord-351964-hduv0ur4 110 4 , , , cord-351964-hduv0ur4 110 5 budded bud VBN cord-351964-hduv0ur4 110 6 into into IN cord-351964-hduv0ur4 110 7 pre pre JJ cord-351964-hduv0ur4 110 8 - - JJ cord-351964-hduv0ur4 110 9 Golgi golgi JJ cord-351964-hduv0ur4 110 10 compartments compartment NNS cord-351964-hduv0ur4 110 11 , , , cord-351964-hduv0ur4 110 12 can can MD cord-351964-hduv0ur4 110 13 be be VB cord-351964-hduv0ur4 110 14 seen see VBN cord-351964-hduv0ur4 110 15 in in IN cord-351964-hduv0ur4 110 16 the the DT cord-351964-hduv0ur4 110 17 dilated dilated JJ cord-351964-hduv0ur4 110 18 rims rim NNS cord-351964-hduv0ur4 110 19 of of IN cord-351964-hduv0ur4 110 20 the the DT cord-351964-hduv0ur4 110 21 Golgi Golgi NNP cord-351964-hduv0ur4 110 22 cisternae cisternae NNP cord-351964-hduv0ur4 110 23 and and CC cord-351964-hduv0ur4 110 24 accumulating accumulate VBG cord-351964-hduv0ur4 110 25 in in IN cord-351964-hduv0ur4 110 26 dilations dilation NNS cord-351964-hduv0ur4 110 27 of of IN cord-351964-hduv0ur4 110 28 the the DT cord-351964-hduv0ur4 110 29 trans trans NNP cord-351964-hduv0ur4 110 30 - - NNP cord-351964-hduv0ur4 110 31 Golgi Golgi NNP cord-351964-hduv0ur4 110 32 network network NN cord-351964-hduv0ur4 110 33 ( ( -LRB- cord-351964-hduv0ur4 110 34 Figs Figs NNP cord-351964-hduv0ur4 110 35 . . . cord-351964-hduv0ur4 110 36 1 1 CD cord-351964-hduv0ur4 110 37 and and CC cord-351964-hduv0ur4 110 38 2 2 CD cord-351964-hduv0ur4 110 39 ) ) -RRB- cord-351964-hduv0ur4 110 40 . . . cord-351964-hduv0ur4 111 1 From from IN cord-351964-hduv0ur4 111 2 the the DT cord-351964-hduv0ur4 111 3 trans trans NNP cord-351964-hduv0ur4 111 4 - - NNP cord-351964-hduv0ur4 111 5 Golgi Golgi NNP cord-351964-hduv0ur4 111 6 network network NN cord-351964-hduv0ur4 111 7 virions virion NNS cord-351964-hduv0ur4 111 8 are be VBP cord-351964-hduv0ur4 111 9 transported transport VBN cord-351964-hduv0ur4 111 10 , , , cord-351964-hduv0ur4 111 11 packed pack VBN cord-351964-hduv0ur4 111 12 into into IN cord-351964-hduv0ur4 111 13 spherical spherical JJ cord-351964-hduv0ur4 111 14 vesicles vesicle NNS cord-351964-hduv0ur4 111 15 , , , cord-351964-hduv0ur4 111 16 to to IN cord-351964-hduv0ur4 111 17 the the DT cord-351964-hduv0ur4 111 18 cell cell NN cord-351964-hduv0ur4 111 19 surface surface NN cord-351964-hduv0ur4 111 20 . . . cord-351964-hduv0ur4 112 1 At at IN cord-351964-hduv0ur4 112 2 these these DT cord-351964-hduv0ur4 112 3 early early JJ cord-351964-hduv0ur4 112 4 times time NNS cord-351964-hduv0ur4 112 5 postinfection postinfection NN cord-351964-hduv0ur4 113 1 the the DT cord-351964-hduv0ur4 113 2 morphology morphology NN cord-351964-hduv0ur4 113 3 of of IN cord-351964-hduv0ur4 113 4 the the DT cord-351964-hduv0ur4 113 5 Golgi Golgi NNP cord-351964-hduv0ur4 113 6 stack stack NN cord-351964-hduv0ur4 113 7 and and CC cord-351964-hduv0ur4 113 8 trans trans JJ cord-351964-hduv0ur4 113 9 - - JJ cord-351964-hduv0ur4 113 10 Golgi Golgi NNP cord-351964-hduv0ur4 113 11 network network NN cord-351964-hduv0ur4 113 12 is be VBZ cord-351964-hduv0ur4 113 13 normal normal JJ cord-351964-hduv0ur4 113 14 and and CC cord-351964-hduv0ur4 113 15 , , , cord-351964-hduv0ur4 113 16 except except IN cord-351964-hduv0ur4 113 17 for for IN cord-351964-hduv0ur4 113 18 the the DT cord-351964-hduv0ur4 113 19 presence presence NN cord-351964-hduv0ur4 113 20 of of IN cord-351964-hduv0ur4 113 21 virions virion NNS cord-351964-hduv0ur4 113 22 , , , cord-351964-hduv0ur4 113 23 identical identical JJ cord-351964-hduv0ur4 113 24 to to IN cord-351964-hduv0ur4 113 25 that that DT cord-351964-hduv0ur4 113 26 in in IN cord-351964-hduv0ur4 113 27 uninfected uninfected JJ cord-351964-hduv0ur4 113 28 cells cell NNS cord-351964-hduv0ur4 113 29 ( ( -LRB- cord-351964-hduv0ur4 113 30 see see VB cord-351964-hduv0ur4 113 31 also also RB cord-351964-hduv0ur4 113 32 Figs fig NNS cord-351964-hduv0ur4 113 33 . . . cord-351964-hduv0ur4 114 1 7 7 CD cord-351964-hduv0ur4 114 2 and and CC cord-351964-hduv0ur4 114 3 8) 8) NN cord-351964-hduv0ur4 114 4 . . . cord-351964-hduv0ur4 115 1 It -PRON- PRP cord-351964-hduv0ur4 115 2 is be VBZ cord-351964-hduv0ur4 115 3 noteworthy noteworthy JJ cord-351964-hduv0ur4 115 4 that that IN cord-351964-hduv0ur4 115 5 in in IN cord-351964-hduv0ur4 115 6 AtT20 AtT20 NNP cord-351964-hduv0ur4 115 7 cells cell NNS cord-351964-hduv0ur4 115 8 and and CC cord-351964-hduv0ur4 115 9 also also RB cord-351964-hduv0ur4 115 10 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 115 11 host host NN cord-351964-hduv0ur4 115 12 cells cell VBZ cord-351964-hduv0ur4 115 13 the the DT cord-351964-hduv0ur4 115 14 coronavirions coronavirion NNS cord-351964-hduv0ur4 115 15 invariably invariably RB cord-351964-hduv0ur4 115 16 occur occur VBP cord-351964-hduv0ur4 115 17 in in IN cord-351964-hduv0ur4 115 18 the the DT cord-351964-hduv0ur4 115 19 dilated dilated JJ cord-351964-hduv0ur4 115 20 rims rim NNS cord-351964-hduv0ur4 115 21 of of IN cord-351964-hduv0ur4 115 22 Golgi Golgi NNP cord-351964-hduv0ur4 115 23 cistemae cistemae NNP cord-351964-hduv0ur4 115 24 and and CC cord-351964-hduv0ur4 115 25 not not RB cord-351964-hduv0ur4 115 26 in in IN cord-351964-hduv0ur4 115 27 the the DT cord-351964-hduv0ur4 115 28 comparatively comparatively RB cord-351964-hduv0ur4 115 29 flat flat JJ cord-351964-hduv0ur4 115 30 stacked stack VBN cord-351964-hduv0ur4 115 31 central central JJ cord-351964-hduv0ur4 115 32 regions region NNS cord-351964-hduv0ur4 115 33 of of IN cord-351964-hduv0ur4 115 34 the the DT cord-351964-hduv0ur4 115 35 cisternae cisternae NNP cord-351964-hduv0ur4 115 36 . . . cord-351964-hduv0ur4 116 1 As as IN cord-351964-hduv0ur4 116 2 Figs fig NNS cord-351964-hduv0ur4 116 3 . . . cord-351964-hduv0ur4 117 1 1 1 CD cord-351964-hduv0ur4 117 2 , , , cord-351964-hduv0ur4 117 3 2 2 CD cord-351964-hduv0ur4 117 4 , , , cord-351964-hduv0ur4 117 5 and and CC cord-351964-hduv0ur4 117 6 7 7 CD cord-351964-hduv0ur4 117 7 show show NN cord-351964-hduv0ur4 117 8 , , , cord-351964-hduv0ur4 117 9 virions virion NNS cord-351964-hduv0ur4 117 10 in in IN cord-351964-hduv0ur4 117 11 the the DT cord-351964-hduv0ur4 117 12 trans trans NNP cord-351964-hduv0ur4 117 13 - - NNP cord-351964-hduv0ur4 117 14 Golgi Golgi NNP cord-351964-hduv0ur4 117 15 network network NN cord-351964-hduv0ur4 117 16 are be VBP cord-351964-hduv0ur4 117 17 often often RB cord-351964-hduv0ur4 117 18 kidney kidney NN cord-351964-hduv0ur4 117 19 shaped shape VBN cord-351964-hduv0ur4 117 20 and and CC cord-351964-hduv0ur4 117 21 have have VBP cord-351964-hduv0ur4 117 22 a a DT cord-351964-hduv0ur4 117 23 fairly fairly RB cord-351964-hduv0ur4 117 24 uniform uniform JJ cord-351964-hduv0ur4 117 25 and and CC cord-351964-hduv0ur4 117 26 high high JJ cord-351964-hduv0ur4 117 27 electron electron NN cord-351964-hduv0ur4 117 28 density density NN cord-351964-hduv0ur4 117 29 . . . cord-351964-hduv0ur4 118 1 By by IN cord-351964-hduv0ur4 118 2 contrast contrast NN cord-351964-hduv0ur4 118 3 the the DT cord-351964-hduv0ur4 118 4 virions virion NNS cord-351964-hduv0ur4 118 5 in in IN cord-351964-hduv0ur4 118 6 the the DT cord-351964-hduv0ur4 118 7 Golgi Golgi NNP cord-351964-hduv0ur4 118 8 cisternae cisternae NNP cord-351964-hduv0ur4 118 9 and and CC cord-351964-hduv0ur4 118 10 pre pre JJ cord-351964-hduv0ur4 118 11 - - JJ cord-351964-hduv0ur4 118 12 Golgi golgi JJ cord-351964-hduv0ur4 118 13 compartments compartment NNS cord-351964-hduv0ur4 118 14 are be VBP cord-351964-hduv0ur4 118 15 spherical spherical JJ cord-351964-hduv0ur4 118 16 structures structure NNS cord-351964-hduv0ur4 118 17 with with IN cord-351964-hduv0ur4 118 18 ribonucleoprotein ribonucleoprotein NN cord-351964-hduv0ur4 118 19 core core NN cord-351964-hduv0ur4 118 20 material material NN cord-351964-hduv0ur4 118 21 immediately immediately RB cord-351964-hduv0ur4 118 22 below below IN cord-351964-hduv0ur4 118 23 the the DT cord-351964-hduv0ur4 118 24 viral viral JJ cord-351964-hduv0ur4 118 25 envelope envelope NN cord-351964-hduv0ur4 118 26 and and CC cord-351964-hduv0ur4 118 27 an an DT cord-351964-hduv0ur4 118 28 " " `` cord-351964-hduv0ur4 118 29 empty empty JJ cord-351964-hduv0ur4 118 30 " " '' cord-351964-hduv0ur4 118 31 center center NN cord-351964-hduv0ur4 118 32 . . . cord-351964-hduv0ur4 119 1 This this DT cord-351964-hduv0ur4 119 2 morphological morphological JJ cord-351964-hduv0ur4 119 3 maturation maturation NN cord-351964-hduv0ur4 119 4 of of IN cord-351964-hduv0ur4 119 5 the the DT cord-351964-hduv0ur4 119 6 virus virus NN cord-351964-hduv0ur4 119 7 , , , cord-351964-hduv0ur4 119 8 which which WDT cord-351964-hduv0ur4 119 9 also also RB cord-351964-hduv0ur4 119 10 occurs occur VBZ cord-351964-hduv0ur4 119 11 in in IN cord-351964-hduv0ur4 119 12 other other JJ cord-351964-hduv0ur4 119 13 host host NN cord-351964-hduv0ur4 119 14 - - HYPH cord-351964-hduv0ur4 119 15 cell cell NN cord-351964-hduv0ur4 119 16 types type NNS cord-351964-hduv0ur4 119 17 ( ( -LRB- cord-351964-hduv0ur4 119 18 for for IN cord-351964-hduv0ur4 119 19 review review NN cord-351964-hduv0ur4 119 20 see see VB cord-351964-hduv0ur4 119 21 Dubois Dubois NNP cord-351964-hduv0ur4 119 22 - - HYPH cord-351964-hduv0ur4 119 23 Dalcq Dalcq NNP cord-351964-hduv0ur4 119 24 et et NNP cord-351964-hduv0ur4 119 25 al al NNP cord-351964-hduv0ur4 119 26 . . NNP cord-351964-hduv0ur4 119 27 , , , cord-351964-hduv0ur4 119 28 1984 1984 CD cord-351964-hduv0ur4 119 29 ) ) -RRB- cord-351964-hduv0ur4 119 30 , , , cord-351964-hduv0ur4 119 31 is be VBZ cord-351964-hduv0ur4 119 32 particularly particularly RB cord-351964-hduv0ur4 119 33 pronounced pronounce VBN cord-351964-hduv0ur4 119 34 in in IN cord-351964-hduv0ur4 119 35 AtT20 AtT20 NNP cord-351964-hduv0ur4 119 36 cells cell NNS cord-351964-hduv0ur4 119 37 , , , cord-351964-hduv0ur4 119 38 and and CC cord-351964-hduv0ur4 119 39 at at IN cord-351964-hduv0ur4 119 40 early early JJ cord-351964-hduv0ur4 119 41 times time NNS cord-351964-hduv0ur4 119 42 postinfection postinfection NN cord-351964-hduv0ur4 119 43 is be VBZ cord-351964-hduv0ur4 119 44 strictly strictly RB cord-351964-hduv0ur4 119 45 correlated correlate VBN cord-351964-hduv0ur4 119 46 with with IN cord-351964-hduv0ur4 119 47 delivery delivery NN cord-351964-hduv0ur4 119 48 of of IN cord-351964-hduv0ur4 119 49 the the DT cord-351964-hduv0ur4 119 50 viruses virus NNS cord-351964-hduv0ur4 119 51 to to IN cord-351964-hduv0ur4 119 52 the the DT cord-351964-hduv0ur4 119 53 trans trans NNP cord-351964-hduv0ur4 119 54 - - NNP cord-351964-hduv0ur4 119 55 Golgi Golgi NNP cord-351964-hduv0ur4 119 56 network network NN cord-351964-hduv0ur4 119 57 . . . cord-351964-hduv0ur4 120 1 Virions virion NNS cord-351964-hduv0ur4 120 2 with with IN cord-351964-hduv0ur4 120 3 the the DT cord-351964-hduv0ur4 120 4 mature mature JJ cord-351964-hduv0ur4 120 5 morphology morphology NN cord-351964-hduv0ur4 120 6 are be VBP cord-351964-hduv0ur4 120 7 , , , cord-351964-hduv0ur4 120 8 therefore therefore RB cord-351964-hduv0ur4 120 9 , , , cord-351964-hduv0ur4 120 10 a a DT cord-351964-hduv0ur4 120 11 convenient convenient JJ cord-351964-hduv0ur4 120 12 and and CC cord-351964-hduv0ur4 120 13 reliable reliable JJ cord-351964-hduv0ur4 120 14 morphological morphological JJ cord-351964-hduv0ur4 120 15 marker marker NN cord-351964-hduv0ur4 120 16 of of IN cord-351964-hduv0ur4 120 17 transand transand JJ cord-351964-hduv0ur4 120 18 post post JJ cord-351964-hduv0ur4 120 19 - - JJ cord-351964-hduv0ur4 120 20 Golgi Golgi NNP cord-351964-hduv0ur4 120 21 compartments compartment NNS cord-351964-hduv0ur4 120 22 . . . cord-351964-hduv0ur4 121 1 To to TO cord-351964-hduv0ur4 121 2 establish establish VB cord-351964-hduv0ur4 121 3 that that DT cord-351964-hduv0ur4 121 4 at at IN cord-351964-hduv0ur4 121 5 between between IN cord-351964-hduv0ur4 121 6 8 8 CD cord-351964-hduv0ur4 121 7 - - SYM cord-351964-hduv0ur4 121 8 10 10 CD cord-351964-hduv0ur4 121 9 h h NN cord-351964-hduv0ur4 121 10 postinfection postinfection NN cord-351964-hduv0ur4 121 11 the the DT cord-351964-hduv0ur4 121 12 Golgi Golgi NNP cord-351964-hduv0ur4 121 13 apparatus apparatus NN cord-351964-hduv0ur4 121 14 is be VBZ cord-351964-hduv0ur4 121 15 still still RB cord-351964-hduv0ur4 121 16 biochemically biochemically RB cord-351964-hduv0ur4 121 17 competent competent JJ cord-351964-hduv0ur4 121 18 , , , cord-351964-hduv0ur4 121 19 as as RB cord-351964-hduv0ur4 121 20 well well RB cord-351964-hduv0ur4 121 21 as as IN cord-351964-hduv0ur4 121 22 mor mor NN cord-351964-hduv0ur4 121 23 - - HYPH cord-351964-hduv0ur4 121 24 phologicaUy phologicaUy NNS cord-351964-hduv0ur4 121 25 intact intact JJ cord-351964-hduv0ur4 121 26 , , , cord-351964-hduv0ur4 121 27 we -PRON- PRP cord-351964-hduv0ur4 121 28 assayed assay VBD cord-351964-hduv0ur4 121 29 its -PRON- PRP$ cord-351964-hduv0ur4 121 30 ability ability NN cord-351964-hduv0ur4 121 31 to to IN cord-351964-hduv0ur4 121 32 O o NN cord-351964-hduv0ur4 121 33 - - HYPH cord-351964-hduv0ur4 121 34 glycosylate glycosylate JJ cord-351964-hduv0ur4 121 35 the the DT cord-351964-hduv0ur4 121 36 E1 e1 NN cord-351964-hduv0ur4 121 37 glycoprotein glycoprotein NN cord-351964-hduv0ur4 121 38 of of IN cord-351964-hduv0ur4 121 39 MHV MHV NNP cord-351964-hduv0ur4 121 40 - - HYPH cord-351964-hduv0ur4 121 41 A59 A59 NNP cord-351964-hduv0ur4 121 42 . . . cord-351964-hduv0ur4 122 1 The the DT cord-351964-hduv0ur4 122 2 only only RB cord-351964-hduv0ur4 122 3 known know VBN cord-351964-hduv0ur4 122 4 posttranslational posttranslational JJ cord-351964-hduv0ur4 122 5 modification modification NN cord-351964-hduv0ur4 122 6 of of IN cord-351964-hduv0ur4 122 7 the the DT cord-351964-hduv0ur4 122 8 transmembrane transmembrane NN cord-351964-hduv0ur4 122 9 E1 e1 NN cord-351964-hduv0ur4 122 10 glycoprotein glycoprotein NN cord-351964-hduv0ur4 122 11 is be VBZ cord-351964-hduv0ur4 122 12 the the DT cord-351964-hduv0ur4 122 13 addition addition NN cord-351964-hduv0ur4 122 14 of of IN cord-351964-hduv0ur4 122 15 O o NN cord-351964-hduv0ur4 122 16 - - HYPH cord-351964-hduv0ur4 122 17 linked link VBN cord-351964-hduv0ur4 122 18 glycans glycan NNS cord-351964-hduv0ur4 122 19 during during IN cord-351964-hduv0ur4 122 20 transit transit NN cord-351964-hduv0ur4 122 21 of of IN cord-351964-hduv0ur4 122 22 the the DT cord-351964-hduv0ur4 122 23 Golgi Golgi NNP cord-351964-hduv0ur4 122 24 apparatus apparatus NN cord-351964-hduv0ur4 122 25 ( ( -LRB- cord-351964-hduv0ur4 122 26 Niemann Niemann NNP cord-351964-hduv0ur4 122 27 and and CC cord-351964-hduv0ur4 122 28 Klenk Klenk NNP cord-351964-hduv0ur4 122 29 , , , cord-351964-hduv0ur4 122 30 1981 1981 CD cord-351964-hduv0ur4 122 31 ) ) -RRB- cord-351964-hduv0ur4 122 32 . . . cord-351964-hduv0ur4 123 1 In in IN cord-351964-hduv0ur4 123 2 fibroblasts fibroblast NNS cord-351964-hduv0ur4 123 3 these these DT cord-351964-hduv0ur4 123 4 are be VBP cord-351964-hduv0ur4 123 5 added add VBN cord-351964-hduv0ur4 123 6 posttranslationally posttranslationally RB cord-351964-hduv0ur4 123 7 to to IN cord-351964-hduv0ur4 123 8 serine serine NN cord-351964-hduv0ur4 123 9 and and CC cord-351964-hduv0ur4 123 10 threonine threonine NN cord-351964-hduv0ur4 123 11 residues residue NNS cord-351964-hduv0ur4 123 12 at at IN cord-351964-hduv0ur4 123 13 the the DT cord-351964-hduv0ur4 123 14 amino amino NN cord-351964-hduv0ur4 123 15 terminus terminus NN cord-351964-hduv0ur4 123 16 of of IN cord-351964-hduv0ur4 123 17 the the DT cord-351964-hduv0ur4 123 18 protein protein NN cord-351964-hduv0ur4 124 1 ( ( -LRB- cord-351964-hduv0ur4 124 2 Niemann Niemann NNP cord-351964-hduv0ur4 124 3 et et NNP cord-351964-hduv0ur4 124 4 al al NNP cord-351964-hduv0ur4 124 5 . . NNP cord-351964-hduv0ur4 124 6 , , , cord-351964-hduv0ur4 124 7 1984 1984 CD cord-351964-hduv0ur4 124 8 ) ) -RRB- cord-351964-hduv0ur4 124 9 , , , cord-351964-hduv0ur4 124 10 which which WDT cord-351964-hduv0ur4 124 11 is be VBZ cord-351964-hduv0ur4 124 12 exposed expose VBN cord-351964-hduv0ur4 124 13 on on IN cord-351964-hduv0ur4 124 14 the the DT cord-351964-hduv0ur4 124 15 lumenal lumenal JJ cord-351964-hduv0ur4 124 16 surface surface NN cord-351964-hduv0ur4 124 17 of of IN cord-351964-hduv0ur4 124 18 intracel- intracel- NN cord-351964-hduv0ur4 124 19 AtT20 AtT20 NNP cord-351964-hduv0ur4 124 20 cells cell NNS cord-351964-hduv0ur4 124 21 . . . cord-351964-hduv0ur4 125 1 Infected infected JJ cord-351964-hduv0ur4 125 2 cells cell NNS cord-351964-hduv0ur4 125 3 were be VBD cord-351964-hduv0ur4 125 4 pulsed pulse VBN cord-351964-hduv0ur4 125 5 for for IN cord-351964-hduv0ur4 125 6 10 10 CD cord-351964-hduv0ur4 125 7 rain rain NN cord-351964-hduv0ur4 125 8 with with IN cord-351964-hduv0ur4 125 9 [ [ -LRB- cord-351964-hduv0ur4 125 10 35S]methionine 35s]methionine CD cord-351964-hduv0ur4 125 11 and and CC cord-351964-hduv0ur4 125 12 then then RB cord-351964-hduv0ur4 125 13 chased chase VBD cord-351964-hduv0ur4 125 14 for for IN cord-351964-hduv0ur4 125 15 the the DT cord-351964-hduv0ur4 125 16 times time NNS cord-351964-hduv0ur4 125 17 indicated indicate VBD cord-351964-hduv0ur4 125 18 , , , cord-351964-hduv0ur4 125 19 up up IN cord-351964-hduv0ur4 125 20 to to TO cord-351964-hduv0ur4 125 21 80 80 CD cord-351964-hduv0ur4 125 22 min min NN cord-351964-hduv0ur4 125 23 . . . cord-351964-hduv0ur4 126 1 The the DT cord-351964-hduv0ur4 126 2 E1 e1 NN cord-351964-hduv0ur4 126 3 glycoprotein glycoprotein NN cord-351964-hduv0ur4 126 4 of of IN cord-351964-hduv0ur4 126 5 MHV MHV NNP cord-351964-hduv0ur4 126 6 - - HYPH cord-351964-hduv0ur4 126 7 A59 A59 NNP cord-351964-hduv0ur4 126 8 was be VBD cord-351964-hduv0ur4 126 9 then then RB cord-351964-hduv0ur4 126 10 immunoprecipitated immunoprecipitate VBN cord-351964-hduv0ur4 126 11 from from IN cord-351964-hduv0ur4 126 12 detergent detergent NN cord-351964-hduv0ur4 126 13 - - HYPH cord-351964-hduv0ur4 126 14 solubilized solubilize VBN cord-351964-hduv0ur4 126 15 cells cell NNS cord-351964-hduv0ur4 126 16 and and CC cord-351964-hduv0ur4 126 17 analyzed analyze VBN cord-351964-hduv0ur4 126 18 by by IN cord-351964-hduv0ur4 126 19 SDS SDS NNP cord-351964-hduv0ur4 126 20 - - HYPH cord-351964-hduv0ur4 126 21 PAGE PAGE NNP cord-351964-hduv0ur4 126 22 . . . cord-351964-hduv0ur4 127 1 During during IN cord-351964-hduv0ur4 127 2 the the DT cord-351964-hduv0ur4 127 3 10-min 10-min CD cord-351964-hduv0ur4 127 4 pulse pulse NN cord-351964-hduv0ur4 127 5 , , , cord-351964-hduv0ur4 127 6 some some DT cord-351964-hduv0ur4 127 7 of of IN cord-351964-hduv0ur4 127 8 the the DT cord-351964-hduv0ur4 127 9 El El NNP cord-351964-hduv0ur4 127 10 becomes become VBZ cord-351964-hduv0ur4 127 11 partially partially RB cord-351964-hduv0ur4 127 12 glyeosylated glyeosylate VBN cord-351964-hduv0ur4 127 13 ( ( -LRB- cord-351964-hduv0ur4 127 14 first first JJ cord-351964-hduv0ur4 127 15 lane lane NN cord-351964-hduv0ur4 127 16 ) ) -RRB- cord-351964-hduv0ur4 127 17 . . . cord-351964-hduv0ur4 128 1 By by IN cord-351964-hduv0ur4 128 2 40 40 CD cord-351964-hduv0ur4 128 3 min min NN cord-351964-hduv0ur4 128 4 of of IN cord-351964-hduv0ur4 128 5 chase chase NNP cord-351964-hduv0ur4 128 6 virtually virtually RB cord-351964-hduv0ur4 128 7 all all DT cord-351964-hduv0ur4 128 8 of of IN cord-351964-hduv0ur4 128 9 the the DT cord-351964-hduv0ur4 128 10 primary primary JJ cord-351964-hduv0ur4 128 11 translation translation NN cord-351964-hduv0ur4 128 12 product product NN cord-351964-hduv0ur4 128 13 has have VBZ cord-351964-hduv0ur4 128 14 chased chase VBN cord-351964-hduv0ur4 128 15 into into IN cord-351964-hduv0ur4 128 16 the the DT cord-351964-hduv0ur4 128 17 two two CD cord-351964-hduv0ur4 128 18 higher high JJR cord-351964-hduv0ur4 128 19 molecular molecular JJ cord-351964-hduv0ur4 128 20 weight weight NN cord-351964-hduv0ur4 128 21 forms form NNS cord-351964-hduv0ur4 128 22 . . . cord-351964-hduv0ur4 129 1 As as IN cord-351964-hduv0ur4 129 2 shown show VBN cord-351964-hduv0ur4 129 3 for for IN cord-351964-hduv0ur4 129 4 the the DT cord-351964-hduv0ur4 129 5 60-min 60-min CD cord-351964-hduv0ur4 129 6 sample sample NN cord-351964-hduv0ur4 129 7 , , , cord-351964-hduv0ur4 129 8 digestion digestion NN cord-351964-hduv0ur4 129 9 with with IN cord-351964-hduv0ur4 129 10 neumminidase neumminidase NN cord-351964-hduv0ur4 129 11 converts convert VBZ cord-351964-hduv0ur4 129 12 the the DT cord-351964-hduv0ur4 129 13 highest high JJS cord-351964-hduv0ur4 129 14 molecular molecular JJ cord-351964-hduv0ur4 129 15 weight weight NN cord-351964-hduv0ur4 129 16 form form NN cord-351964-hduv0ur4 129 17 to to IN cord-351964-hduv0ur4 129 18 an an DT cord-351964-hduv0ur4 129 19 intermediate intermediate JJ cord-351964-hduv0ur4 129 20 form form NN cord-351964-hduv0ur4 129 21 , , , cord-351964-hduv0ur4 129 22 indicating indicate VBG cord-351964-hduv0ur4 129 23 that that IN cord-351964-hduv0ur4 129 24 the the DT cord-351964-hduv0ur4 129 25 former former JJ cord-351964-hduv0ur4 129 26 is be VBZ cord-351964-hduv0ur4 129 27 the the DT cord-351964-hduv0ur4 129 28 sialylated sialylated JJ cord-351964-hduv0ur4 129 29 or or CC cord-351964-hduv0ur4 129 30 fully fully RB cord-351964-hduv0ur4 129 31 glycosylated glycosylate VBN cord-351964-hduv0ur4 129 32 form form NN cord-351964-hduv0ur4 129 33 of of IN cord-351964-hduv0ur4 129 34 the the DT cord-351964-hduv0ur4 129 35 protein protein NN cord-351964-hduv0ur4 129 36 . . . cord-351964-hduv0ur4 130 1 At at IN cord-351964-hduv0ur4 130 2 8-h 8-h CD cord-351964-hduv0ur4 130 3 postinfection postinfection NN cord-351964-hduv0ur4 130 4 AtT20 AtT20 NNP cord-351964-hduv0ur4 130 5 cells cell NNS cord-351964-hduv0ur4 130 6 were be VBD cord-351964-hduv0ur4 130 7 pulsed pulse VBN cord-351964-hduv0ur4 130 8 for for IN cord-351964-hduv0ur4 130 9 10 10 CD cord-351964-hduv0ur4 130 10 min min NN cord-351964-hduv0ur4 130 11 with with IN cord-351964-hduv0ur4 130 12 [ [ -LRB- cord-351964-hduv0ur4 130 13 35S]methionine 35s]methionine CD cord-351964-hduv0ur4 130 14 and and CC cord-351964-hduv0ur4 130 15 then then RB cord-351964-hduv0ur4 130 16 chased chase VBD cord-351964-hduv0ur4 130 17 for for IN cord-351964-hduv0ur4 130 18 up up IN cord-351964-hduv0ur4 130 19 to to TO cord-351964-hduv0ur4 130 20 80 80 CD cord-351964-hduv0ur4 130 21 min min NN cord-351964-hduv0ur4 130 22 . . . cord-351964-hduv0ur4 131 1 Fig Fig NNP cord-351964-hduv0ur4 131 2 . . . cord-351964-hduv0ur4 131 3 _SP cord-351964-hduv0ur4 132 1 3 3 CD cord-351964-hduv0ur4 132 2 shows show VBZ cord-351964-hduv0ur4 132 3 the the DT cord-351964-hduv0ur4 132 4 result result NN cord-351964-hduv0ur4 132 5 . . . cord-351964-hduv0ur4 133 1 After after IN cord-351964-hduv0ur4 133 2 the the DT cord-351964-hduv0ur4 133 3 10-min 10-min CD cord-351964-hduv0ur4 133 4 labeling labeling NN cord-351964-hduv0ur4 133 5 the the DT cord-351964-hduv0ur4 133 6 E1 e1 NN cord-351964-hduv0ur4 133 7 glycoprotein glycoprotein NN cord-351964-hduv0ur4 133 8 is be VBZ cord-351964-hduv0ur4 133 9 resolved resolve VBN cord-351964-hduv0ur4 133 10 on on IN cord-351964-hduv0ur4 133 11 polyacrylamide polyacrylamide JJ cord-351964-hduv0ur4 133 12 gels gel NNS cord-351964-hduv0ur4 133 13 into into IN cord-351964-hduv0ur4 133 14 two two CD cord-351964-hduv0ur4 133 15 bands band NNS cord-351964-hduv0ur4 133 16 . . . cord-351964-hduv0ur4 134 1 After after IN cord-351964-hduv0ur4 134 2 a a DT cord-351964-hduv0ur4 134 3 10-min 10-min CD cord-351964-hduv0ur4 134 4 chase chase NN cord-351964-hduv0ur4 134 5 a a DT cord-351964-hduv0ur4 134 6 third third JJ cord-351964-hduv0ur4 134 7 higher high JJR cord-351964-hduv0ur4 134 8 molecular molecular JJ cord-351964-hduv0ur4 134 9 weight weight NN cord-351964-hduv0ur4 134 10 band band NN cord-351964-hduv0ur4 134 11 is be VBZ cord-351964-hduv0ur4 134 12 resolved resolve VBN cord-351964-hduv0ur4 134 13 . . . cord-351964-hduv0ur4 135 1 These these DT cord-351964-hduv0ur4 135 2 data datum NNS cord-351964-hduv0ur4 135 3 indicate indicate VBP cord-351964-hduv0ur4 135 4 that that IN cord-351964-hduv0ur4 135 5 , , , cord-351964-hduv0ur4 135 6 as as IN cord-351964-hduv0ur4 135 7 in in IN cord-351964-hduv0ur4 135 8 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 135 9 saccells saccell NNS cord-351964-hduv0ur4 135 10 ( ( -LRB- cord-351964-hduv0ur4 135 11 Tooze Tooze NNP cord-351964-hduv0ur4 135 12 , , , cord-351964-hduv0ur4 135 13 S. S. NNP cord-351964-hduv0ur4 135 14 A. a. NN cord-351964-hduv0ur4 135 15 , , , cord-351964-hduv0ur4 135 16 J. J. NNP cord-351964-hduv0ur4 135 17 Tooze Tooze NNP cord-351964-hduv0ur4 135 18 , , , cord-351964-hduv0ur4 135 19 and and CC cord-351964-hduv0ur4 135 20 G. G. NNP cord-351964-hduv0ur4 135 21 Warren Warren NNP cord-351964-hduv0ur4 135 22 , , , cord-351964-hduv0ur4 135 23 manuscript manuscript NN cord-351964-hduv0ur4 135 24 in in IN cord-351964-hduv0ur4 135 25 preparation preparation NN cord-351964-hduv0ur4 135 26 ) ) -RRB- cord-351964-hduv0ur4 135 27 , , , cord-351964-hduv0ur4 135 28 the the DT cord-351964-hduv0ur4 135 29 O o NN cord-351964-hduv0ur4 135 30 - - HYPH cord-351964-hduv0ur4 135 31 glycosylation glycosylation NN cord-351964-hduv0ur4 135 32 of of IN cord-351964-hduv0ur4 135 33 E1 e1 NN cord-351964-hduv0ur4 135 34 occurs occur VBZ cord-351964-hduv0ur4 135 35 in in RP cord-351964-hduv0ur4 135 36 at at RB cord-351964-hduv0ur4 135 37 least least RBS cord-351964-hduv0ur4 135 38 two two CD cord-351964-hduv0ur4 135 39 steps step NNS cord-351964-hduv0ur4 135 40 and and CC cord-351964-hduv0ur4 135 41 that that IN cord-351964-hduv0ur4 135 42 some some DT cord-351964-hduv0ur4 135 43 E1 e1 NN cord-351964-hduv0ur4 135 44 molecules molecule NNS cord-351964-hduv0ur4 135 45 are be VBP cord-351964-hduv0ur4 135 46 partially partially RB cord-351964-hduv0ur4 135 47 glycosylated glycosylate VBN cord-351964-hduv0ur4 135 48 by by IN cord-351964-hduv0ur4 135 49 the the DT cord-351964-hduv0ur4 135 50 end end NN cord-351964-hduv0ur4 135 51 of of IN cord-351964-hduv0ur4 135 52 a a DT cord-351964-hduv0ur4 135 53 10-min 10-min CD cord-351964-hduv0ur4 135 54 pulse pulse NN cord-351964-hduv0ur4 135 55 . . . cord-351964-hduv0ur4 136 1 By by IN cord-351964-hduv0ur4 136 2 counting count VBG cord-351964-hduv0ur4 136 3 the the DT cord-351964-hduv0ur4 136 4 radioactivity radioactivity NN cord-351964-hduv0ur4 136 5 in in IN cord-351964-hduv0ur4 136 6 the the DT cord-351964-hduv0ur4 136 7 three three CD cord-351964-hduv0ur4 136 8 bands band NNS cord-351964-hduv0ur4 136 9 during during IN cord-351964-hduv0ur4 136 10 the the DT cord-351964-hduv0ur4 136 11 chase chase NN cord-351964-hduv0ur4 136 12 we -PRON- PRP cord-351964-hduv0ur4 136 13 found find VBD cord-351964-hduv0ur4 136 14 that that IN cord-351964-hduv0ur4 136 15 within within IN cord-351964-hduv0ur4 136 16 80 80 CD cord-351964-hduv0ur4 136 17 min min NN cord-351964-hduv0ur4 136 18 of of IN cord-351964-hduv0ur4 136 19 its -PRON- PRP$ cord-351964-hduv0ur4 136 20 synthesis synthesis NN cord-351964-hduv0ur4 136 21 , , , cord-351964-hduv0ur4 136 22 40 40 CD cord-351964-hduv0ur4 136 23 - - SYM cord-351964-hduv0ur4 136 24 45 45 CD cord-351964-hduv0ur4 136 25 % % NN cord-351964-hduv0ur4 136 26 of of IN cord-351964-hduv0ur4 136 27 the the DT cord-351964-hduv0ur4 136 28 E1 e1 NN cord-351964-hduv0ur4 136 29 is be VBZ cord-351964-hduv0ur4 136 30 found find VBN cord-351964-hduv0ur4 136 31 in in IN cord-351964-hduv0ur4 136 32 the the DT cord-351964-hduv0ur4 136 33 highest high JJS cord-351964-hduv0ur4 136 34 molecular molecular JJ cord-351964-hduv0ur4 136 35 weight weight NN cord-351964-hduv0ur4 136 36 form form NN cord-351964-hduv0ur4 136 37 and and CC cord-351964-hduv0ur4 136 38 presumably presumably RB cord-351964-hduv0ur4 136 39 is be VBZ cord-351964-hduv0ur4 136 40 fully fully RB cord-351964-hduv0ur4 136 41 glycosylated glycosylate VBN cord-351964-hduv0ur4 136 42 . . . cord-351964-hduv0ur4 137 1 About about IN cord-351964-hduv0ur4 137 2 the the DT cord-351964-hduv0ur4 137 3 same same JJ cord-351964-hduv0ur4 137 4 percentage percentage NN cord-351964-hduv0ur4 137 5 has have VBZ cord-351964-hduv0ur4 137 6 been be VBN cord-351964-hduv0ur4 137 7 partially partially RB cord-351964-hduv0ur4 137 8 glycosylated glycosylate VBN cord-351964-hduv0ur4 137 9 . . . cord-351964-hduv0ur4 138 1 To to TO cord-351964-hduv0ur4 138 2 establish establish VB cord-351964-hduv0ur4 138 3 that that IN cord-351964-hduv0ur4 138 4 the the DT cord-351964-hduv0ur4 138 5 highest high JJS cord-351964-hduv0ur4 138 6 molecular molecular JJ cord-351964-hduv0ur4 138 7 weight weight NN cord-351964-hduv0ur4 138 8 species specie NNS cord-351964-hduv0ur4 138 9 had have VBD cord-351964-hduv0ur4 138 10 terminal terminal JJ cord-351964-hduv0ur4 138 11 sialic sialic NN cord-351964-hduv0ur4 138 12 residues residue NNS cord-351964-hduv0ur4 138 13 on on IN cord-351964-hduv0ur4 138 14 the the DT cord-351964-hduv0ur4 138 15 sugar sugar NN cord-351964-hduv0ur4 138 16 chains chain NNS cord-351964-hduv0ur4 138 17 , , , cord-351964-hduv0ur4 138 18 and and CC cord-351964-hduv0ur4 138 19 therefore therefore RB cord-351964-hduv0ur4 138 20 was be VBD cord-351964-hduv0ur4 138 21 fully fully RB cord-351964-hduv0ur4 138 22 glycosylated glycosylate VBN cord-351964-hduv0ur4 138 23 , , , cord-351964-hduv0ur4 138 24 an an DT cord-351964-hduv0ur4 138 25 aliquot aliquot NN cord-351964-hduv0ur4 138 26 of of IN cord-351964-hduv0ur4 138 27 E1 e1 NN cord-351964-hduv0ur4 138 28 was be VBD cord-351964-hduv0ur4 138 29 taken take VBN cord-351964-hduv0ur4 138 30 after after IN cord-351964-hduv0ur4 138 31 the the DT cord-351964-hduv0ur4 138 32 60-min 60-min CD cord-351964-hduv0ur4 138 33 chase chase NN cord-351964-hduv0ur4 138 34 . . . cord-351964-hduv0ur4 139 1 One one CD cord-351964-hduv0ur4 139 2 - - HYPH cord-351964-hduv0ur4 139 3 half half NN cord-351964-hduv0ur4 139 4 was be VBD cord-351964-hduv0ur4 139 5 digested digest VBN cord-351964-hduv0ur4 139 6 with with IN cord-351964-hduv0ur4 139 7 neuraminidase neuraminidase NN cord-351964-hduv0ur4 139 8 while while IN cord-351964-hduv0ur4 139 9 the the DT cord-351964-hduv0ur4 139 10 other other JJ cord-351964-hduv0ur4 139 11 was be VBD cord-351964-hduv0ur4 139 12 similarly similarly RB cord-351964-hduv0ur4 139 13 incubated incubate VBN cord-351964-hduv0ur4 139 14 but but CC cord-351964-hduv0ur4 139 15 without without IN cord-351964-hduv0ur4 139 16 neuraminidase neuraminidase NN cord-351964-hduv0ur4 139 17 . . . cord-351964-hduv0ur4 140 1 This this DT cord-351964-hduv0ur4 140 2 digestion digestion NN cord-351964-hduv0ur4 140 3 converted convert VBD cord-351964-hduv0ur4 140 4 the the DT cord-351964-hduv0ur4 140 5 highest high JJS cord-351964-hduv0ur4 140 6 molecular molecular JJ cord-351964-hduv0ur4 140 7 weight weight NN cord-351964-hduv0ur4 140 8 form form NN cord-351964-hduv0ur4 140 9 to to IN cord-351964-hduv0ur4 140 10 an an DT cord-351964-hduv0ur4 140 11 intermediate intermediate JJ cord-351964-hduv0ur4 140 12 form form NN cord-351964-hduv0ur4 140 13 ( ( -LRB- cord-351964-hduv0ur4 140 14 Fig fig NN cord-351964-hduv0ur4 140 15 . . . cord-351964-hduv0ur4 140 16 3 3 CD cord-351964-hduv0ur4 140 17 ) ) -RRB- cord-351964-hduv0ur4 140 18 . . . cord-351964-hduv0ur4 141 1 These these DT cord-351964-hduv0ur4 141 2 results result NNS cord-351964-hduv0ur4 141 3 show show VBP cord-351964-hduv0ur4 141 4 that that IN cord-351964-hduv0ur4 141 5 at at IN cord-351964-hduv0ur4 141 6 8 8 CD cord-351964-hduv0ur4 141 7 - - SYM cord-351964-hduv0ur4 141 8 10-h 10-h CD cord-351964-hduv0ur4 141 9 postinfection postinfection NN cord-351964-hduv0ur4 141 10 the the DT cord-351964-hduv0ur4 141 11 Golgi Golgi NNP cord-351964-hduv0ur4 141 12 apparatus apparatus NN cord-351964-hduv0ur4 141 13 is be VBZ cord-351964-hduv0ur4 141 14 biochemically biochemically RB cord-351964-hduv0ur4 141 15 , , , cord-351964-hduv0ur4 141 16 as as RB cord-351964-hduv0ur4 141 17 well well RB cord-351964-hduv0ur4 141 18 as as IN cord-351964-hduv0ur4 141 19 morphologically morphologically RB cord-351964-hduv0ur4 141 20 , , , cord-351964-hduv0ur4 141 21 intact intact JJ cord-351964-hduv0ur4 141 22 and and CC cord-351964-hduv0ur4 141 23 adds add VBZ cord-351964-hduv0ur4 141 24 O o NN cord-351964-hduv0ur4 141 25 - - HYPH cord-351964-hduv0ur4 141 26 linked link VBN cord-351964-hduv0ur4 141 27 sugar sugar NN cord-351964-hduv0ur4 141 28 chains chain NNS cord-351964-hduv0ur4 141 29 terminating terminate VBG cord-351964-hduv0ur4 141 30 in in IN cord-351964-hduv0ur4 141 31 sialic sialic JJ cord-351964-hduv0ur4 141 32 acid acid NN cord-351964-hduv0ur4 141 33 residues residue NNS cord-351964-hduv0ur4 141 34 to to IN cord-351964-hduv0ur4 141 35 the the DT cord-351964-hduv0ur4 141 36 viral viral JJ cord-351964-hduv0ur4 141 37 E1 e1 NN cord-351964-hduv0ur4 141 38 glycoprotein glycoprotein NN cord-351964-hduv0ur4 141 39 . . . cord-351964-hduv0ur4 142 1 To to TO cord-351964-hduv0ur4 142 2 provide provide VB cord-351964-hduv0ur4 142 3 further further JJ cord-351964-hduv0ur4 142 4 evidence evidence NN cord-351964-hduv0ur4 142 5 that that IN cord-351964-hduv0ur4 142 6 the the DT cord-351964-hduv0ur4 142 7 constitutive constitutive JJ cord-351964-hduv0ur4 142 8 pathway pathway NN cord-351964-hduv0ur4 142 9 functions function NNS cord-351964-hduv0ur4 142 10 normally normally RB cord-351964-hduv0ur4 142 11 at at IN cord-351964-hduv0ur4 142 12 early early JJ cord-351964-hduv0ur4 142 13 times time NNS cord-351964-hduv0ur4 142 14 after after IN cord-351964-hduv0ur4 142 15 infection infection NN cord-351964-hduv0ur4 142 16 with with IN cord-351964-hduv0ur4 142 17 MHV MHV NNP cord-351964-hduv0ur4 142 18 - - HYPH cord-351964-hduv0ur4 142 19 A59 A59 NNP cord-351964-hduv0ur4 142 20 we -PRON- PRP cord-351964-hduv0ur4 142 21 performed perform VBD cord-351964-hduv0ur4 142 22 the the DT cord-351964-hduv0ur4 142 23 following follow VBG cord-351964-hduv0ur4 142 24 double double JJ cord-351964-hduv0ur4 142 25 - - HYPH cord-351964-hduv0ur4 142 26 infection infection NN cord-351964-hduv0ur4 142 27 experiment experiment NN cord-351964-hduv0ur4 142 28 . . . cord-351964-hduv0ur4 143 1 ART20 ART20 NNP cord-351964-hduv0ur4 143 2 cells cell NNS cord-351964-hduv0ur4 143 3 growing grow VBG cord-351964-hduv0ur4 143 4 on on IN cord-351964-hduv0ur4 143 5 ECM ECM NNP cord-351964-hduv0ur4 143 6 - - HYPH cord-351964-hduv0ur4 143 7 coated coat VBN cord-351964-hduv0ur4 143 8 glass glass NN cord-351964-hduv0ur4 143 9 coverslips coverslip NNS cord-351964-hduv0ur4 143 10 were be VBD cord-351964-hduv0ur4 143 11 infected infect VBN cord-351964-hduv0ur4 143 12 with with IN cord-351964-hduv0ur4 143 13 MHV MHV NNP cord-351964-hduv0ur4 143 14 - - HYPH cord-351964-hduv0ur4 143 15 A59 A59 NNP cord-351964-hduv0ur4 143 16 for for IN cord-351964-hduv0ur4 143 17 2 2 CD cord-351964-hduv0ur4 143 18 h h NN cord-351964-hduv0ur4 143 19 at at IN cord-351964-hduv0ur4 143 20 3"/~ 3"/~ CD cord-351964-hduv0ur4 144 1 The the DT cord-351964-hduv0ur4 144 2 cells cell NNS cord-351964-hduv0ur4 144 3 were be VBD cord-351964-hduv0ur4 144 4 then then RB cord-351964-hduv0ur4 144 5 washed wash VBN cord-351964-hduv0ur4 144 6 and and CC cord-351964-hduv0ur4 144 7 incubated incubate VBN cord-351964-hduv0ur4 144 8 at at IN cord-351964-hduv0ur4 144 9 370C 370c CD cord-351964-hduv0ur4 144 10 for for IN cord-351964-hduv0ur4 144 11 2.5 2.5 CD cord-351964-hduv0ur4 144 12 h h NN cord-351964-hduv0ur4 144 13 until until IN cord-351964-hduv0ur4 144 14 4.5-h 4.5-h CD cord-351964-hduv0ur4 144 15 postinfection postinfection NN cord-351964-hduv0ur4 144 16 . . . cord-351964-hduv0ur4 145 1 The the DT cord-351964-hduv0ur4 145 2 cultures culture NNS cord-351964-hduv0ur4 145 3 were be VBD cord-351964-hduv0ur4 145 4 then then RB cord-351964-hduv0ur4 145 5 superinfected superinfect VBN cord-351964-hduv0ur4 145 6 with with IN cord-351964-hduv0ur4 145 7 VSV VSV NNP cord-351964-hduv0ur4 145 8 for for IN cord-351964-hduv0ur4 145 9 I I NNP cord-351964-hduv0ur4 145 10 h h NNP cord-351964-hduv0ur4 145 11 and and CC cord-351964-hduv0ur4 145 12 incubated incubate VBN cord-351964-hduv0ur4 145 13 for for IN cord-351964-hduv0ur4 145 14 a a DT cord-351964-hduv0ur4 145 15 further further JJ cord-351964-hduv0ur4 145 16 2.5 2.5 CD cord-351964-hduv0ur4 145 17 h h NN cord-351964-hduv0ur4 145 18 at at IN cord-351964-hduv0ur4 145 19 37~ 37~ CD cord-351964-hduv0ur4 146 1 They -PRON- PRP cord-351964-hduv0ur4 146 2 were be VBD cord-351964-hduv0ur4 146 3 then then RB cord-351964-hduv0ur4 146 4 fixed fix VBN cord-351964-hduv0ur4 146 5 , , , cord-351964-hduv0ur4 146 6 8 8 CD cord-351964-hduv0ur4 146 7 h h NN cord-351964-hduv0ur4 146 8 after after IN cord-351964-hduv0ur4 146 9 infection infection NN cord-351964-hduv0ur4 146 10 with with IN cord-351964-hduv0ur4 146 11 MHV MHV NNP cord-351964-hduv0ur4 146 12 - - HYPH cord-351964-hduv0ur4 146 13 A59 A59 NNP cord-351964-hduv0ur4 146 14 and and CC cord-351964-hduv0ur4 146 15 3.5 3.5 CD cord-351964-hduv0ur4 146 16 h h NN cord-351964-hduv0ur4 146 17 after after IN cord-351964-hduv0ur4 146 18 infection infection NN cord-351964-hduv0ur4 146 19 with with IN cord-351964-hduv0ur4 146 20 VSV VSV NNP cord-351964-hduv0ur4 146 21 , , , cord-351964-hduv0ur4 146 22 and and CC cord-351964-hduv0ur4 146 23 processed process VBN cord-351964-hduv0ur4 146 24 for for IN cord-351964-hduv0ur4 146 25 immunofluorescence immunofluorescence NN cord-351964-hduv0ur4 146 26 microscopy microscopy NNS cord-351964-hduv0ur4 146 27 using use VBG cord-351964-hduv0ur4 146 28 a a DT cord-351964-hduv0ur4 146 29 double double JJ cord-351964-hduv0ur4 146 30 - - HYPH cord-351964-hduv0ur4 146 31 labeling labeling NN cord-351964-hduv0ur4 146 32 procedure procedure NN cord-351964-hduv0ur4 146 33 with with IN cord-351964-hduv0ur4 146 34 a a DT cord-351964-hduv0ur4 146 35 rabbit rabbit NN cord-351964-hduv0ur4 146 36 antiserum antiserum NN cord-351964-hduv0ur4 146 37 against against IN cord-351964-hduv0ur4 146 38 VSV VSV NNP cord-351964-hduv0ur4 146 39 - - HYPH cord-351964-hduv0ur4 146 40 G G NNP cord-351964-hduv0ur4 146 41 protein protein NN cord-351964-hduv0ur4 146 42 and and CC cord-351964-hduv0ur4 146 43 an an DT cord-351964-hduv0ur4 146 44 affinity affinity NN cord-351964-hduv0ur4 146 45 - - HYPH cord-351964-hduv0ur4 146 46 purified purify VBN cord-351964-hduv0ur4 146 47 mouse mouse NN cord-351964-hduv0ur4 146 48 monoclonal monoclonal JJ cord-351964-hduv0ur4 146 49 antibody antibody NN cord-351964-hduv0ur4 146 50 against against IN cord-351964-hduv0ur4 146 51 E1 e1 NN cord-351964-hduv0ur4 146 52 of of IN cord-351964-hduv0ur4 146 53 MHV MHV NNP cord-351964-hduv0ur4 146 54 - - HYPH cord-351964-hduv0ur4 146 55 A59 A59 NNP cord-351964-hduv0ur4 146 56 . . . cord-351964-hduv0ur4 147 1 All all DT cord-351964-hduv0ur4 147 2 of of IN cord-351964-hduv0ur4 147 3 the the DT cord-351964-hduv0ur4 147 4 cells cell NNS cord-351964-hduv0ur4 147 5 were be VBD cord-351964-hduv0ur4 147 6 infected infect VBN cord-351964-hduv0ur4 147 7 by by IN cord-351964-hduv0ur4 147 8 the the DT cord-351964-hduv0ur4 147 9 VSV VSV NNP cord-351964-hduv0ur4 147 10 and and CC cord-351964-hduv0ur4 147 11 expressed express VBD cord-351964-hduv0ur4 147 12 G g NN cord-351964-hduv0ur4 147 13 protein protein NN cord-351964-hduv0ur4 147 14 at at IN cord-351964-hduv0ur4 147 15 their -PRON- PRP$ cord-351964-hduv0ur4 147 16 cell cell NN cord-351964-hduv0ur4 147 17 surfaces surface NNS cord-351964-hduv0ur4 147 18 , , , cord-351964-hduv0ur4 147 19 including include VBG cord-351964-hduv0ur4 147 20 the the DT cord-351964-hduv0ur4 147 21 25 25 CD cord-351964-hduv0ur4 147 22 - - SYM cord-351964-hduv0ur4 147 23 30 30 CD cord-351964-hduv0ur4 147 24 % % NN cord-351964-hduv0ur4 147 25 of of IN cord-351964-hduv0ur4 147 26 cells cell NNS cord-351964-hduv0ur4 147 27 that that WDT cord-351964-hduv0ur4 147 28 had have VBD cord-351964-hduv0ur4 147 29 been be VBN cord-351964-hduv0ur4 147 30 infected infect VBN cord-351964-hduv0ur4 147 31 by by IN cord-351964-hduv0ur4 147 32 MHV MHV NNP cord-351964-hduv0ur4 147 33 - - HYPH cord-351964-hduv0ur4 147 34 A59 A59 NNP cord-351964-hduv0ur4 147 35 and and CC cord-351964-hduv0ur4 147 36 therefore therefore RB cord-351964-hduv0ur4 147 37 contained contain VBD cord-351964-hduv0ur4 147 38 the the DT cord-351964-hduv0ur4 147 39 coronaviral coronaviral JJ cord-351964-hduv0ur4 147 40 E1 e1 NN cord-351964-hduv0ur4 147 41 glycoprotein glycoprotein NN cord-351964-hduv0ur4 147 42 accumulated accumulate VBN cord-351964-hduv0ur4 147 43 in in IN cord-351964-hduv0ur4 147 44 the the DT cord-351964-hduv0ur4 147 45 Golgi Golgi NNP cord-351964-hduv0ur4 147 46 region region NN cord-351964-hduv0ur4 147 47 ( ( -LRB- cord-351964-hduv0ur4 147 48 Fig Fig NNP cord-351964-hduv0ur4 147 49 . . NNP cord-351964-hduv0ur4 147 50 4 4 CD cord-351964-hduv0ur4 147 51 ) ) -RRB- cord-351964-hduv0ur4 147 52 . . . cord-351964-hduv0ur4 148 1 This this DT cord-351964-hduv0ur4 148 2 establishes establish VBZ cord-351964-hduv0ur4 148 3 that that IN cord-351964-hduv0ur4 148 4 between between IN cord-351964-hduv0ur4 148 5 5.5-and 5.5-and CD cord-351964-hduv0ur4 148 6 8-h 8-h CD cord-351964-hduv0ur4 148 7 postinfection postinfection NN cord-351964-hduv0ur4 148 8 with with IN cord-351964-hduv0ur4 148 9 the the DT cord-351964-hduv0ur4 148 10 coronavirus coronavirus NN cord-351964-hduv0ur4 148 11 the the DT cord-351964-hduv0ur4 148 12 constitutive constitutive JJ cord-351964-hduv0ur4 148 13 exocytic exocytic JJ cord-351964-hduv0ur4 148 14 pathway pathway NN cord-351964-hduv0ur4 148 15 of of IN cord-351964-hduv0ur4 148 16 AtT20 AtT20 NNP cord-351964-hduv0ur4 148 17 cells cell NNS cord-351964-hduv0ur4 148 18 continues continue VBZ cord-351964-hduv0ur4 148 19 to to TO cord-351964-hduv0ur4 148 20 function function VB cord-351964-hduv0ur4 148 21 and and CC cord-351964-hduv0ur4 148 22 can can MD cord-351964-hduv0ur4 148 23 transport transport VB cord-351964-hduv0ur4 148 24 VSV VSV NNP cord-351964-hduv0ur4 148 25 - - HYPH cord-351964-hduv0ur4 148 26 G G NNP cord-351964-hduv0ur4 148 27 protein protein NN cord-351964-hduv0ur4 148 28 to to IN cord-351964-hduv0ur4 148 29 the the DT cord-351964-hduv0ur4 148 30 plasma plasma NN cord-351964-hduv0ur4 148 31 membrane membrane NN cord-351964-hduv0ur4 148 32 . . . cord-351964-hduv0ur4 149 1 The the DT cord-351964-hduv0ur4 149 2 same same JJ cord-351964-hduv0ur4 149 3 is be VBZ cord-351964-hduv0ur4 149 4 true true JJ cord-351964-hduv0ur4 149 5 for for IN cord-351964-hduv0ur4 149 6 cells cell NNS cord-351964-hduv0ur4 149 7 of of IN cord-351964-hduv0ur4 149 8 the the DT cord-351964-hduv0ur4 149 9 murine murine JJ cord-351964-hduv0ur4 149 10 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 149 11 line line NN cord-351964-hduv0ur4 149 12 sac-(data sac-(data NNP cord-351964-hduv0ur4 149 13 not not RB cord-351964-hduv0ur4 149 14 shown show VBN cord-351964-hduv0ur4 149 15 ) ) -RRB- cord-351964-hduv0ur4 149 16 . . . cord-351964-hduv0ur4 150 1 We -PRON- PRP cord-351964-hduv0ur4 150 2 used use VBD cord-351964-hduv0ur4 150 3 the the DT cord-351964-hduv0ur4 150 4 well well NN cord-351964-hduv0ur4 150 5 characterized characterized JJ cord-351964-hduv0ur4 150 6 N n NN cord-351964-hduv0ur4 150 7 - - HYPH cord-351964-hduv0ur4 150 8 glycosylation glycosylation NN cord-351964-hduv0ur4 150 9 of of IN cord-351964-hduv0ur4 150 10 VSV VSV NNP cord-351964-hduv0ur4 150 11 G G NNP cord-351964-hduv0ur4 150 12 protein protein NN cord-351964-hduv0ur4 150 13 to to TO cord-351964-hduv0ur4 150 14 test test VB cord-351964-hduv0ur4 150 15 further far RBR cord-351964-hduv0ur4 150 16 the the DT cord-351964-hduv0ur4 150 17 integrity integrity NN cord-351964-hduv0ur4 150 18 of of IN cord-351964-hduv0ur4 150 19 its -PRON- PRP$ cord-351964-hduv0ur4 150 20 transport transport NN cord-351964-hduv0ur4 150 21 to to IN cord-351964-hduv0ur4 150 22 the the DT cord-351964-hduv0ur4 150 23 plasma plasma NN cord-351964-hduv0ur4 150 24 membrane membrane NN cord-351964-hduv0ur4 150 25 surface surface NN cord-351964-hduv0ur4 150 26 in in IN cord-351964-hduv0ur4 150 27 MHV mhv NN cord-351964-hduv0ur4 150 28 - - HYPH cord-351964-hduv0ur4 150 29 infected infect VBN cord-351964-hduv0ur4 150 30 cells cell NNS cord-351964-hduv0ur4 150 31 ( ( -LRB- cord-351964-hduv0ur4 150 32 Fig fig NN cord-351964-hduv0ur4 150 33 . . . cord-351964-hduv0ur4 150 34 5 5 CD cord-351964-hduv0ur4 150 35 ) ) -RRB- cord-351964-hduv0ur4 150 36 . . . cord-351964-hduv0ur4 151 1 Knipe Knipe NNP cord-351964-hduv0ur4 151 2 et et NNP cord-351964-hduv0ur4 151 3 al al NNP cord-351964-hduv0ur4 151 4 . . . cord-351964-hduv0ur4 152 1 ( ( -LRB- cord-351964-hduv0ur4 152 2 1977 1977 CD cord-351964-hduv0ur4 152 3 ) ) -RRB- cord-351964-hduv0ur4 152 4 originally originally RB cord-351964-hduv0ur4 152 5 showed show VBD cord-351964-hduv0ur4 152 6 that that IN cord-351964-hduv0ur4 152 7 the the DT cord-351964-hduv0ur4 152 8 increase increase NN cord-351964-hduv0ur4 152 9 in in IN cord-351964-hduv0ur4 152 10 apparent apparent JJ cord-351964-hduv0ur4 152 11 molecular molecular JJ cord-351964-hduv0ur4 152 12 weight weight NN cord-351964-hduv0ur4 152 13 of of IN cord-351964-hduv0ur4 152 14 the the DT cord-351964-hduv0ur4 152 15 G g NN cord-351964-hduv0ur4 152 16 protein protein NN cord-351964-hduv0ur4 152 17 marked mark VBD cord-351964-hduv0ur4 152 18 the the DT cord-351964-hduv0ur4 152 19 acquisition acquisition NN cord-351964-hduv0ur4 152 20 of of IN cord-351964-hduv0ur4 152 21 terminal terminal JJ cord-351964-hduv0ur4 152 22 sialic sialic NN cord-351964-hduv0ur4 152 23 acid acid NN cord-351964-hduv0ur4 152 24 , , , cord-351964-hduv0ur4 152 25 the the DT cord-351964-hduv0ur4 152 26 last last JJ cord-351964-hduv0ur4 152 27 identified identify VBN cord-351964-hduv0ur4 152 28 step step NN cord-351964-hduv0ur4 152 29 in in IN cord-351964-hduv0ur4 152 30 the the DT cord-351964-hduv0ur4 152 31 Golgi Golgi NNP cord-351964-hduv0ur4 152 32 processing processing NN cord-351964-hduv0ur4 152 33 . . . cord-351964-hduv0ur4 153 1 There there EX cord-351964-hduv0ur4 153 2 were be VBD cord-351964-hduv0ur4 153 3 no no DT cord-351964-hduv0ur4 153 4 detectable detectable JJ cord-351964-hduv0ur4 153 5 differences difference NNS cord-351964-hduv0ur4 153 6 in in IN cord-351964-hduv0ur4 153 7 the the DT cord-351964-hduv0ur4 153 8 kinetics kinetic NNS cord-351964-hduv0ur4 153 9 of of IN cord-351964-hduv0ur4 153 10 this this DT cord-351964-hduv0ur4 153 11 apparent apparent JJ cord-351964-hduv0ur4 153 12 molecular molecular JJ cord-351964-hduv0ur4 153 13 weight weight NN cord-351964-hduv0ur4 153 14 shift shift NN cord-351964-hduv0ur4 153 15 in in IN cord-351964-hduv0ur4 153 16 the the DT cord-351964-hduv0ur4 153 17 presence presence NN cord-351964-hduv0ur4 153 18 or or CC cord-351964-hduv0ur4 153 19 the the DT cord-351964-hduv0ur4 153 20 absence absence NN cord-351964-hduv0ur4 153 21 of of IN cord-351964-hduv0ur4 153 22 MHV MHV NNP cord-351964-hduv0ur4 153 23 infection infection NN cord-351964-hduv0ur4 153 24 implying imply VBG cord-351964-hduv0ur4 153 25 that that IN cord-351964-hduv0ur4 153 26 neither neither CC cord-351964-hduv0ur4 153 27 the the DT cord-351964-hduv0ur4 153 28 rate rate NN cord-351964-hduv0ur4 153 29 nor nor CC cord-351964-hduv0ur4 153 30 extent extent NN cord-351964-hduv0ur4 153 31 of of IN cord-351964-hduv0ur4 153 32 sialylation sialylation NN cord-351964-hduv0ur4 153 33 are be VBP cord-351964-hduv0ur4 153 34 affected affect VBN cord-351964-hduv0ur4 153 35 ( ( -LRB- cord-351964-hduv0ur4 153 36 Fig Fig NNP cord-351964-hduv0ur4 153 37 . . . cord-351964-hduv0ur4 153 38 5 5 CD cord-351964-hduv0ur4 153 39 ) ) -RRB- cord-351964-hduv0ur4 153 40 . . . cord-351964-hduv0ur4 154 1 Hence hence RB cord-351964-hduv0ur4 154 2 the the DT cord-351964-hduv0ur4 154 3 processing processing NN cord-351964-hduv0ur4 154 4 of of IN cord-351964-hduv0ur4 154 5 a a DT cord-351964-hduv0ur4 154 6 plasma plasma NN cord-351964-hduv0ur4 154 7 membrane membrane NN cord-351964-hduv0ur4 154 8 glycoprotein glycoprotein NN cord-351964-hduv0ur4 154 9 seems seem VBZ cord-351964-hduv0ur4 154 10 to to TO cord-351964-hduv0ur4 154 11 be be VB cord-351964-hduv0ur4 154 12 unaffected unaffected JJ cord-351964-hduv0ur4 154 13 by by IN cord-351964-hduv0ur4 154 14 up up IN cord-351964-hduv0ur4 154 15 to to TO cord-351964-hduv0ur4 154 16 8 8 CD cord-351964-hduv0ur4 154 17 h h NN cord-351964-hduv0ur4 154 18 of of IN cord-351964-hduv0ur4 154 19 MHV MHV NNP cord-351964-hduv0ur4 154 20 - - HYPH cord-351964-hduv0ur4 154 21 A59 A59 NNP cord-351964-hduv0ur4 154 22 infection infection NN cord-351964-hduv0ur4 154 23 . . . cord-351964-hduv0ur4 155 1 Using use VBG cord-351964-hduv0ur4 155 2 an an DT cord-351964-hduv0ur4 155 3 antibody antibody NN cord-351964-hduv0ur4 155 4 specific specific JJ cord-351964-hduv0ur4 155 5 for for IN cord-351964-hduv0ur4 155 6 the the DT cord-351964-hduv0ur4 155 7 carboxy carboxy JJ cord-351964-hduv0ur4 155 8 - - HYPH cord-351964-hduv0ur4 155 9 terminal terminal NN cord-351964-hduv0ur4 155 10 domain domain NN cord-351964-hduv0ur4 155 11 of of IN cord-351964-hduv0ur4 155 12 the the DT cord-351964-hduv0ur4 155 13 glycoprotein glycoprotein NN cord-351964-hduv0ur4 155 14 E1 e1 NN cord-351964-hduv0ur4 155 15 of of IN cord-351964-hduv0ur4 155 16 MHV MHV NNP cord-351964-hduv0ur4 155 17 - - HYPH cord-351964-hduv0ur4 155 18 A59 A59 NNP cord-351964-hduv0ur4 156 1 ( ( -LRB- cord-351964-hduv0ur4 156 2 Tooze Tooze NNP cord-351964-hduv0ur4 156 3 and and CC cord-351964-hduv0ur4 156 4 Stanley Stanley NNP cord-351964-hduv0ur4 156 5 , , , cord-351964-hduv0ur4 156 6 1986 1986 CD cord-351964-hduv0ur4 156 7 ) ) -RRB- cord-351964-hduv0ur4 157 1 we -PRON- PRP cord-351964-hduv0ur4 157 2 labeled label VBD cord-351964-hduv0ur4 157 3 MHV MHV NNP cord-351964-hduv0ur4 157 4 - - HYPH cord-351964-hduv0ur4 157 5 A59-infected A59-infected NNP cord-351964-hduv0ur4 157 6 AtT20 AtT20 NNP cord-351964-hduv0ur4 157 7 cells cell NNS cord-351964-hduv0ur4 157 8 using use VBG cord-351964-hduv0ur4 157 9 the the DT cord-351964-hduv0ur4 157 10 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 157 11 procedure procedure NN cord-351964-hduv0ur4 157 12 . . . cord-351964-hduv0ur4 158 1 As as IN cord-351964-hduv0ur4 158 2 Fig Fig NNP cord-351964-hduv0ur4 158 3 . . . cord-351964-hduv0ur4 158 4 6 6 CD cord-351964-hduv0ur4 158 5 shows show NNS cord-351964-hduv0ur4 158 6 , , , cord-351964-hduv0ur4 158 7 membranes membrane NNS cord-351964-hduv0ur4 158 8 of of IN cord-351964-hduv0ur4 158 9 the the DT cord-351964-hduv0ur4 158 10 stacked stack VBN cord-351964-hduv0ur4 158 11 Golgi Golgi NNP cord-351964-hduv0ur4 158 12 cisternae cisternae NNP cord-351964-hduv0ur4 158 13 were be VBD cord-351964-hduv0ur4 158 14 heavily heavily RB cord-351964-hduv0ur4 158 15 labeled label VBN cord-351964-hduv0ur4 158 16 on on IN cord-351964-hduv0ur4 158 17 their -PRON- PRP$ cord-351964-hduv0ur4 158 18 The the DT cord-351964-hduv0ur4 158 19 Journal Journal NNP cord-351964-hduv0ur4 158 20 of of IN cord-351964-hduv0ur4 158 21 Cell Cell NNP cord-351964-hduv0ur4 158 22 Biology Biology NNP cord-351964-hduv0ur4 158 23 , , , cord-351964-hduv0ur4 158 24 Volume volume NN cord-351964-hduv0ur4 158 25 105 105 CD cord-351964-hduv0ur4 158 26 , , , cord-351964-hduv0ur4 158 27 1987 1987 CD cord-351964-hduv0ur4 158 28 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 158 29 face face NN cord-351964-hduv0ur4 158 30 ; ; : cord-351964-hduv0ur4 158 31 by by IN cord-351964-hduv0ur4 158 32 contrast contrast NN cord-351964-hduv0ur4 158 33 membranes membrane NNS cord-351964-hduv0ur4 158 34 of of IN cord-351964-hduv0ur4 158 35 the the DT cord-351964-hduv0ur4 158 36 trans trans NNP cord-351964-hduv0ur4 158 37 - - NNP cord-351964-hduv0ur4 158 38 Golgi Golgi NNP cord-351964-hduv0ur4 158 39 network network NN cord-351964-hduv0ur4 158 40 , , , cord-351964-hduv0ur4 158 41 identified identify VBN cord-351964-hduv0ur4 158 42 by by IN cord-351964-hduv0ur4 158 43 the the DT cord-351964-hduv0ur4 158 44 presence presence NN cord-351964-hduv0ur4 158 45 of of IN cord-351964-hduv0ur4 158 46 mature mature JJ cord-351964-hduv0ur4 158 47 virions virion NNS cord-351964-hduv0ur4 158 48 as as RB cord-351964-hduv0ur4 158 49 well well RB cord-351964-hduv0ur4 158 50 as as IN cord-351964-hduv0ur4 158 51 extensive extensive JJ cord-351964-hduv0ur4 158 52 surface surface NN cord-351964-hduv0ur4 158 53 coats coat NNS cord-351964-hduv0ur4 158 54 , , , cord-351964-hduv0ur4 158 55 were be VBD cord-351964-hduv0ur4 158 56 unlabeled unlabeled JJ cord-351964-hduv0ur4 158 57 . . . cord-351964-hduv0ur4 159 1 Post post JJ cord-351964-hduv0ur4 159 2 - - JJ cord-351964-hduv0ur4 159 3 Golgi Golgi NNP cord-351964-hduv0ur4 159 4 vesicles vesicle NNS cord-351964-hduv0ur4 159 5 transporting transport VBG cord-351964-hduv0ur4 159 6 progeny progeny JJ cord-351964-hduv0ur4 159 7 virions virion NNS cord-351964-hduv0ur4 159 8 to to IN cord-351964-hduv0ur4 159 9 the the DT cord-351964-hduv0ur4 159 10 cell cell NN cord-351964-hduv0ur4 159 11 surface surface NN cord-351964-hduv0ur4 159 12 and and CC cord-351964-hduv0ur4 159 13 secretory secretory NN cord-351964-hduv0ur4 159 14 granules granule NNS cord-351964-hduv0ur4 159 15 were be VBD cord-351964-hduv0ur4 159 16 also also RB cord-351964-hduv0ur4 159 17 unlabeled unlabeled JJ cord-351964-hduv0ur4 159 18 ( ( -LRB- cord-351964-hduv0ur4 159 19 not not RB cord-351964-hduv0ur4 159 20 shown show VBN cord-351964-hduv0ur4 159 21 ) ) -RRB- cord-351964-hduv0ur4 159 22 . . . cord-351964-hduv0ur4 160 1 This this DT cord-351964-hduv0ur4 160 2 establishes establish VBZ cord-351964-hduv0ur4 160 3 that that IN cord-351964-hduv0ur4 160 4 , , , cord-351964-hduv0ur4 160 5 at at IN cord-351964-hduv0ur4 160 6 8 8 CD cord-351964-hduv0ur4 160 7 - - SYM cord-351964-hduv0ur4 160 8 10-h 10-h CD cord-351964-hduv0ur4 160 9 postinfection postinfection NN cord-351964-hduv0ur4 160 10 , , , cord-351964-hduv0ur4 160 11 the the DT cord-351964-hduv0ur4 160 12 E1 e1 NN cord-351964-hduv0ur4 160 13 glycoprotein glycoprotein NN cord-351964-hduv0ur4 160 14 in in IN cord-351964-hduv0ur4 160 15 AtT20 AtT20 NNP cord-351964-hduv0ur4 160 16 cells cell NNS cord-351964-hduv0ur4 160 17 enters enter VBZ cord-351964-hduv0ur4 160 18 the the DT cord-351964-hduv0ur4 160 19 exocytic exocytic JJ cord-351964-hduv0ur4 160 20 pathway pathway NN cord-351964-hduv0ur4 160 21 and and CC cord-351964-hduv0ur4 160 22 reaches reach VBZ cord-351964-hduv0ur4 160 23 the the DT cord-351964-hduv0ur4 160 24 membranes membrane NNS cord-351964-hduv0ur4 160 25 of of IN cord-351964-hduv0ur4 160 26 the the DT cord-351964-hduv0ur4 160 27 stacked stack VBN cord-351964-hduv0ur4 160 28 Golgi Golgi NNP cord-351964-hduv0ur4 160 29 cisternae cisternae NNP cord-351964-hduv0ur4 160 30 where where WRB cord-351964-hduv0ur4 160 31 it -PRON- PRP cord-351964-hduv0ur4 160 32 accumulates accumulate VBZ cord-351964-hduv0ur4 160 33 and and CC cord-351964-hduv0ur4 160 34 is be VBZ cord-351964-hduv0ur4 160 35 apparently apparently RB cord-351964-hduv0ur4 160 36 retained retain VBN cord-351964-hduv0ur4 160 37 , , , cord-351964-hduv0ur4 160 38 perhaps perhaps RB cord-351964-hduv0ur4 160 39 being be VBG cord-351964-hduv0ur4 160 40 responsible responsible JJ cord-351964-hduv0ur4 160 41 for for IN cord-351964-hduv0ur4 160 42 the the DT cord-351964-hduv0ur4 160 43 eventual eventual JJ cord-351964-hduv0ur4 160 44 vesicularization vesicularization NN cord-351964-hduv0ur4 160 45 of of IN cord-351964-hduv0ur4 160 46 the the DT cord-351964-hduv0ur4 160 47 Golgi Golgi NNP cord-351964-hduv0ur4 160 48 cisternae cisternae NNP cord-351964-hduv0ur4 160 49 . . . cord-351964-hduv0ur4 161 1 E1 E1 NNP cord-351964-hduv0ur4 161 2 is be VBZ cord-351964-hduv0ur4 161 3 not not RB cord-351964-hduv0ur4 161 4 , , , cord-351964-hduv0ur4 161 5 thereafter thereafter RB cord-351964-hduv0ur4 161 6 , , , cord-351964-hduv0ur4 161 7 transported transport VBN cord-351964-hduv0ur4 161 8 as as IN cord-351964-hduv0ur4 161 9 an an DT cord-351964-hduv0ur4 161 10 integral integral JJ cord-351964-hduv0ur4 161 11 protein protein NN cord-351964-hduv0ur4 161 12 of of IN cord-351964-hduv0ur4 161 13 cellular cellular JJ cord-351964-hduv0ur4 161 14 membranes membrane NNS cord-351964-hduv0ur4 161 15 to to IN cord-351964-hduv0ur4 161 16 the the DT cord-351964-hduv0ur4 161 17 trans trans NNP cord-351964-hduv0ur4 161 18 - - NNP cord-351964-hduv0ur4 161 19 Golgi Golgi NNP cord-351964-hduv0ur4 161 20 network network NN cord-351964-hduv0ur4 161 21 and and CC cord-351964-hduv0ur4 161 22 beyond beyond IN cord-351964-hduv0ur4 161 23 . . . cord-351964-hduv0ur4 162 1 This this DT cord-351964-hduv0ur4 162 2 pattern pattern NN cord-351964-hduv0ur4 162 3 of of IN cord-351964-hduv0ur4 162 4 selective selective JJ cord-351964-hduv0ur4 162 5 E1 e1 NN cord-351964-hduv0ur4 162 6 transport transport NN cord-351964-hduv0ur4 162 7 is be VBZ cord-351964-hduv0ur4 162 8 also also RB cord-351964-hduv0ur4 162 9 seen see VBN cord-351964-hduv0ur4 162 10 in in IN cord-351964-hduv0ur4 162 11 infected infected JJ cord-351964-hduv0ur4 162 12 sacfibroblastic sacfibroblastic JJ cord-351964-hduv0ur4 162 13 cells cell NNS cord-351964-hduv0ur4 162 14 ( ( -LRB- cord-351964-hduv0ur4 162 15 Tooze Tooze NNP cord-351964-hduv0ur4 162 16 and and CC cord-351964-hduv0ur4 162 17 Stanley Stanley NNP cord-351964-hduv0ur4 162 18 , , , cord-351964-hduv0ur4 162 19 1986 1986 CD cord-351964-hduv0ur4 162 20 ) ) -RRB- cord-351964-hduv0ur4 162 21 and and CC cord-351964-hduv0ur4 162 22 provides provide VBZ cord-351964-hduv0ur4 162 23 further further JJ cord-351964-hduv0ur4 162 24 evidence evidence NN cord-351964-hduv0ur4 162 25 that that IN cord-351964-hduv0ur4 162 26 the the DT cord-351964-hduv0ur4 162 27 Golgi Golgi NNP cord-351964-hduv0ur4 162 28 apparatus apparatus NN cord-351964-hduv0ur4 162 29 retains retain VBZ cord-351964-hduv0ur4 162 30 its -PRON- PRP$ cord-351964-hduv0ur4 162 31 functional functional JJ cord-351964-hduv0ur4 162 32 integrity integrity NN cord-351964-hduv0ur4 162 33 at at IN cord-351964-hduv0ur4 162 34 early early JJ cord-351964-hduv0ur4 162 35 times time NNS cord-351964-hduv0ur4 162 36 postinfection postinfection NN cord-351964-hduv0ur4 162 37 . . . cord-351964-hduv0ur4 163 1 Budded Budded NNP cord-351964-hduv0ur4 163 2 virions virion NNS cord-351964-hduv0ur4 163 3 are be VBP cord-351964-hduv0ur4 163 4 not not RB cord-351964-hduv0ur4 163 5 labeled label VBN cord-351964-hduv0ur4 163 6 presumably presumably RB cord-351964-hduv0ur4 163 7 because because IN cord-351964-hduv0ur4 163 8 the the DT cord-351964-hduv0ur4 163 9 carboxy carboxy JJ cord-351964-hduv0ur4 163 10 - - HYPH cord-351964-hduv0ur4 163 11 terminal terminal NN cord-351964-hduv0ur4 163 12 epitopes epitope NNS cord-351964-hduv0ur4 163 13 of of IN cord-351964-hduv0ur4 163 14 E1 E1 NNP cord-351964-hduv0ur4 163 15 recognized recognize VBN cord-351964-hduv0ur4 163 16 by by IN cord-351964-hduv0ur4 163 17 the the DT cord-351964-hduv0ur4 163 18 antibody antibody NN cord-351964-hduv0ur4 163 19 are be VBP cord-351964-hduv0ur4 163 20 masked mask VBN cord-351964-hduv0ur4 163 21 by by IN cord-351964-hduv0ur4 163 22 association association NNP cord-351964-hduv0ur4 163 23 with with IN cord-351964-hduv0ur4 163 24 nucleocapsid nucleocapsid NNP cord-351964-hduv0ur4 163 25 protein protein NNP cord-351964-hduv0ur4 163 26 and and CC cord-351964-hduv0ur4 163 27 genomic genomic JJ cord-351964-hduv0ur4 163 28 RNA RNA NNP cord-351964-hduv0ur4 163 29 on on IN cord-351964-hduv0ur4 163 30 the the DT cord-351964-hduv0ur4 163 31 inner inner JJ cord-351964-hduv0ur4 163 32 face face NN cord-351964-hduv0ur4 163 33 of of IN cord-351964-hduv0ur4 163 34 the the DT cord-351964-hduv0ur4 163 35 virion virion NN cord-351964-hduv0ur4 163 36 's 's POS cord-351964-hduv0ur4 163 37 envelope envelope NN cord-351964-hduv0ur4 163 38 ( ( -LRB- cord-351964-hduv0ur4 163 39 Tooze Tooze NNP cord-351964-hduv0ur4 163 40 and and CC cord-351964-hduv0ur4 163 41 Stanley Stanley NNP cord-351964-hduv0ur4 163 42 , , , cord-351964-hduv0ur4 163 43 1986 1986 CD cord-351964-hduv0ur4 163 44 ) ) -RRB- cord-351964-hduv0ur4 163 45 . . . cord-351964-hduv0ur4 164 1 Condensation condensation NN cord-351964-hduv0ur4 164 2 of of IN cord-351964-hduv0ur4 164 3 secretory secretory JJ cord-351964-hduv0ur4 164 4 proteins protein NNS cord-351964-hduv0ur4 164 5 and and CC cord-351964-hduv0ur4 164 6 the the DT cord-351964-hduv0ur4 164 7 formation formation NN cord-351964-hduv0ur4 164 8 of of IN cord-351964-hduv0ur4 164 9 secretory secretory JJ cord-351964-hduv0ur4 164 10 granules granule NNS cord-351964-hduv0ur4 164 11 of of IN cord-351964-hduv0ur4 164 12 the the DT cord-351964-hduv0ur4 164 13 regulated regulated JJ cord-351964-hduv0ur4 164 14 exocytic exocytic JJ cord-351964-hduv0ur4 164 15 pathway pathway NN cord-351964-hduv0ur4 164 16 occurs occur VBZ cord-351964-hduv0ur4 164 17 exclusively exclusively RB cord-351964-hduv0ur4 164 18 in in IN cord-351964-hduv0ur4 164 19 the the DT cord-351964-hduv0ur4 164 20 trans trans NNP cord-351964-hduv0ur4 164 21 - - NNP cord-351964-hduv0ur4 164 22 Golgi Golgi NNP cord-351964-hduv0ur4 164 23 network network NN cord-351964-hduv0ur4 164 24 in in IN cord-351964-hduv0ur4 164 25 uninfected uninfected JJ cord-351964-hduv0ur4 164 26 AtT20 AtT20 NNP cord-351964-hduv0ur4 164 27 cells cell NNS cord-351964-hduv0ur4 164 28 . . . cord-351964-hduv0ur4 165 1 Progeny progeny JJ cord-351964-hduv0ur4 165 2 coronavirions coronavirion NNS cord-351964-hduv0ur4 165 3 that that WDT cord-351964-hduv0ur4 165 4 have have VBP cord-351964-hduv0ur4 165 5 budded bud VBN cord-351964-hduv0ur4 165 6 in in IN cord-351964-hduv0ur4 165 7 pre pre JJ cord-351964-hduv0ur4 165 8 - - JJ cord-351964-hduv0ur4 165 9 Golgi Golgi NNP cord-351964-hduv0ur4 165 10 compartments compartment NNS cord-351964-hduv0ur4 165 11 traverse traverse VB cord-351964-hduv0ur4 165 12 the the DT cord-351964-hduv0ur4 165 13 Golgi Golgi NNP cord-351964-hduv0ur4 165 14 stacks stack NNS cord-351964-hduv0ur4 165 15 and and CC cord-351964-hduv0ur4 165 16 are be VBP cord-351964-hduv0ur4 165 17 thence thence RB cord-351964-hduv0ur4 165 18 transported transport VBN cord-351964-hduv0ur4 165 19 to to IN cord-351964-hduv0ur4 165 20 the the DT cord-351964-hduv0ur4 165 21 plasma plasma NN cord-351964-hduv0ur4 165 22 membrane membrane NN cord-351964-hduv0ur4 165 23 . . . cord-351964-hduv0ur4 166 1 We -PRON- PRP cord-351964-hduv0ur4 166 2 therefore therefore RB cord-351964-hduv0ur4 166 3 examined examine VBD cord-351964-hduv0ur4 166 4 thin thin JJ cord-351964-hduv0ur4 166 5 sections section NNS cord-351964-hduv0ur4 166 6 of of IN cord-351964-hduv0ur4 166 7 infected infected JJ cord-351964-hduv0ur4 166 8 AtT20 AtT20 NNP cord-351964-hduv0ur4 166 9 cells cell NNS cord-351964-hduv0ur4 166 10 under under IN cord-351964-hduv0ur4 166 11 the the DT cord-351964-hduv0ur4 166 12 electron electron NN cord-351964-hduv0ur4 166 13 microscope microscope NN cord-351964-hduv0ur4 166 14 to to TO cord-351964-hduv0ur4 166 15 determine determine VB cord-351964-hduv0ur4 166 16 which which WDT cord-351964-hduv0ur4 166 17 compartments compartment NNS cord-351964-hduv0ur4 166 18 contained contain VBD cord-351964-hduv0ur4 166 19 both both DT cord-351964-hduv0ur4 166 20 condensing condense VBG cord-351964-hduv0ur4 166 21 secretory secretory NN cord-351964-hduv0ur4 166 22 proteins protein NNS cord-351964-hduv0ur4 166 23 and and CC cord-351964-hduv0ur4 166 24 coronavirus coronavirus NN cord-351964-hduv0ur4 166 25 . . . cord-351964-hduv0ur4 167 1 In in IN cord-351964-hduv0ur4 167 2 many many JJ cord-351964-hduv0ur4 167 3 infected infected JJ cord-351964-hduv0ur4 167 4 cells cell NNS cord-351964-hduv0ur4 167 5 with with IN cord-351964-hduv0ur4 167 6 progeny progeny JJ cord-351964-hduv0ur4 167 7 virions virion NNS cord-351964-hduv0ur4 167 8 in in IN cord-351964-hduv0ur4 167 9 the the DT cord-351964-hduv0ur4 167 10 Golgi Golgi NNP cord-351964-hduv0ur4 167 11 stack stack NN cord-351964-hduv0ur4 167 12 there there EX cord-351964-hduv0ur4 167 13 was be VBD cord-351964-hduv0ur4 167 14 no no DT cord-351964-hduv0ur4 167 15 evidence evidence NN cord-351964-hduv0ur4 167 16 of of IN cord-351964-hduv0ur4 167 17 condensing condense VBG cord-351964-hduv0ur4 167 18 secretory secretory JJ cord-351964-hduv0ur4 167 19 proteins protein NNS cord-351964-hduv0ur4 167 20 in in IN cord-351964-hduv0ur4 167 21 the the DT cord-351964-hduv0ur4 167 22 trans trans NNP cord-351964-hduv0ur4 167 23 - - NNP cord-351964-hduv0ur4 167 24 Golgi Golgi NNP cord-351964-hduv0ur4 167 25 network network NN cord-351964-hduv0ur4 167 26 ( ( -LRB- cord-351964-hduv0ur4 167 27 Fig Fig NNP cord-351964-hduv0ur4 167 28 . . NNP cord-351964-hduv0ur4 167 29 2 2 CD cord-351964-hduv0ur4 167 30 ) ) -RRB- cord-351964-hduv0ur4 167 31 ; ; : cord-351964-hduv0ur4 167 32 since since IN cord-351964-hduv0ur4 167 33 MHV MHV NNP cord-351964-hduv0ur4 167 34 - - HYPH cord-351964-hduv0ur4 167 35 A59 A59 NNP cord-351964-hduv0ur4 167 36 infections infection NNS cord-351964-hduv0ur4 167 37 are be VBP cord-351964-hduv0ur4 167 38 asynchronous asynchronous JJ cord-351964-hduv0ur4 167 39 , , , cord-351964-hduv0ur4 167 40 in in IN cord-351964-hduv0ur4 167 41 some some DT cord-351964-hduv0ur4 167 42 cells cell NNS cord-351964-hduv0ur4 167 43 viral viral JJ cord-351964-hduv0ur4 167 44 protein protein NN cord-351964-hduv0ur4 167 45 synthesis synthesis NN cord-351964-hduv0ur4 167 46 may may MD cord-351964-hduv0ur4 167 47 have have VB cord-351964-hduv0ur4 167 48 competed compete VBN cord-351964-hduv0ur4 167 49 with with IN cord-351964-hduv0ur4 167 50 cellular cellular JJ cord-351964-hduv0ur4 167 51 protein protein NN cord-351964-hduv0ur4 167 52 synthesis synthesis NN cord-351964-hduv0ur4 167 53 . . . cord-351964-hduv0ur4 168 1 In in IN cord-351964-hduv0ur4 168 2 other other JJ cord-351964-hduv0ur4 168 3 cells cell NNS cord-351964-hduv0ur4 168 4 virions virion NNS cord-351964-hduv0ur4 168 5 and and CC cord-351964-hduv0ur4 168 6 condensing condense VBG cord-351964-hduv0ur4 168 7 secretory secretory JJ cord-351964-hduv0ur4 168 8 proteins protein NNS cord-351964-hduv0ur4 168 9 were be VBD cord-351964-hduv0ur4 168 10 in in IN cord-351964-hduv0ur4 168 11 different different JJ cord-351964-hduv0ur4 168 12 dilations dilation NNS cord-351964-hduv0ur4 168 13 of of IN cord-351964-hduv0ur4 168 14 the the DT cord-351964-hduv0ur4 168 15 complex complex JJ cord-351964-hduv0ur4 168 16 trans trans NNP cord-351964-hduv0ur4 168 17 - - NNP cord-351964-hduv0ur4 168 18 Golgi Golgi NNP cord-351964-hduv0ur4 168 19 network network NN cord-351964-hduv0ur4 168 20 ( ( -LRB- cord-351964-hduv0ur4 168 21 Fig Fig NNP cord-351964-hduv0ur4 168 22 . . . cord-351964-hduv0ur4 168 23 1 1 CD cord-351964-hduv0ur4 168 24 ) ) -RRB- cord-351964-hduv0ur4 168 25 . . . cord-351964-hduv0ur4 169 1 In in IN cord-351964-hduv0ur4 169 2 a a DT cord-351964-hduv0ur4 169 3 third third JJ cord-351964-hduv0ur4 169 4 class class NN cord-351964-hduv0ur4 169 5 , , , cord-351964-hduv0ur4 169 6 however however RB cord-351964-hduv0ur4 169 7 , , , cord-351964-hduv0ur4 169 8 clumps clump NNS cord-351964-hduv0ur4 169 9 of of IN cord-351964-hduv0ur4 169 10 condensing condense VBG cord-351964-hduv0ur4 169 11 secretory secretory JJ cord-351964-hduv0ur4 169 12 proteins protein NNS cord-351964-hduv0ur4 169 13 and and CC cord-351964-hduv0ur4 169 14 progeny progeny JJ cord-351964-hduv0ur4 169 15 virions virion NNS cord-351964-hduv0ur4 169 16 occurred occur VBD cord-351964-hduv0ur4 169 17 in in IN cord-351964-hduv0ur4 169 18 the the DT cord-351964-hduv0ur4 169 19 same same JJ cord-351964-hduv0ur4 169 20 regions region NNS cord-351964-hduv0ur4 169 21 of of IN cord-351964-hduv0ur4 169 22 the the DT cord-351964-hduv0ur4 169 23 trans trans NNP cord-351964-hduv0ur4 169 24 - - NNP cord-351964-hduv0ur4 169 25 Golgi Golgi NNP cord-351964-hduv0ur4 169 26 network network NN cord-351964-hduv0ur4 169 27 ( ( -LRB- cord-351964-hduv0ur4 169 28 Fig Fig NNP cord-351964-hduv0ur4 169 29 . . NNP cord-351964-hduv0ur4 169 30 7 7 CD cord-351964-hduv0ur4 169 31 , , , cord-351964-hduv0ur4 169 32 A a NN cord-351964-hduv0ur4 169 33 and and CC cord-351964-hduv0ur4 169 34 _SP cord-351964-hduv0ur4 169 35 B b NN cord-351964-hduv0ur4 169 36 ) ) -RRB- cord-351964-hduv0ur4 169 37 . . . cord-351964-hduv0ur4 170 1 Note note VB cord-351964-hduv0ur4 170 2 that that IN cord-351964-hduv0ur4 170 3 although although IN cord-351964-hduv0ur4 170 4 they -PRON- PRP cord-351964-hduv0ur4 170 5 are be VBP cord-351964-hduv0ur4 170 6 in in IN cord-351964-hduv0ur4 170 7 proximity proximity NN cord-351964-hduv0ur4 170 8 in in IN cord-351964-hduv0ur4 170 9 the the DT cord-351964-hduv0ur4 170 10 same same JJ cord-351964-hduv0ur4 170 11 compartment compartment NN cord-351964-hduv0ur4 170 12 , , , cord-351964-hduv0ur4 170 13 the the DT cord-351964-hduv0ur4 170 14 virions virion NNS cord-351964-hduv0ur4 170 15 do do VBP cord-351964-hduv0ur4 170 16 not not RB cord-351964-hduv0ur4 170 17 occur occur VB cord-351964-hduv0ur4 170 18 embedded embed VBN cord-351964-hduv0ur4 170 19 in in IN cord-351964-hduv0ur4 170 20 or or CC cord-351964-hduv0ur4 170 21 surrounded surround VBN cord-351964-hduv0ur4 170 22 by by IN cord-351964-hduv0ur4 170 23 condensing condense VBG cord-351964-hduv0ur4 170 24 secretory secretory NN cord-351964-hduv0ur4 170 25 proteins protein NNS cord-351964-hduv0ur4 170 26 . . . cord-351964-hduv0ur4 171 1 This this DT cord-351964-hduv0ur4 171 2 separation separation NN cord-351964-hduv0ur4 171 3 is be VBZ cord-351964-hduv0ur4 171 4 maintained maintain VBN cord-351964-hduv0ur4 171 5 as as IN cord-351964-hduv0ur4 171 6 the the DT cord-351964-hduv0ur4 171 7 secretory secretory JJ cord-351964-hduv0ur4 171 8 proteins protein NNS cord-351964-hduv0ur4 171 9 condense condense VBP cord-351964-hduv0ur4 171 10 further further RB cord-351964-hduv0ur4 171 11 into into IN cord-351964-hduv0ur4 171 12 secretory secretory JJ cord-351964-hduv0ur4 171 13 granule granule NN cord-351964-hduv0ur4 171 14 cores core NNS cord-351964-hduv0ur4 171 15 . . . cord-351964-hduv0ur4 172 1 Fig Fig NNP cord-351964-hduv0ur4 172 2 . . NNP cord-351964-hduv0ur4 172 3 7 7 CD cord-351964-hduv0ur4 172 4 B b NN cord-351964-hduv0ur4 172 5 , , , cord-351964-hduv0ur4 172 6 inset inset NN cord-351964-hduv0ur4 172 7 shows show VBZ cord-351964-hduv0ur4 172 8 two two CD cord-351964-hduv0ur4 172 9 morphologically morphologically RB cord-351964-hduv0ur4 172 10 mature mature JJ cord-351964-hduv0ur4 172 11 cores core NNS cord-351964-hduv0ur4 172 12 and and CC cord-351964-hduv0ur4 172 13 two two CD cord-351964-hduv0ur4 172 14 virions virion NNS cord-351964-hduv0ur4 172 15 in in IN cord-351964-hduv0ur4 172 16 the the DT cord-351964-hduv0ur4 172 17 same same JJ cord-351964-hduv0ur4 172 18 trans trans JJ cord-351964-hduv0ur4 172 19 - - NNP cord-351964-hduv0ur4 172 20 Golgi Golgi NNP cord-351964-hduv0ur4 172 21 compartment compartment NN cord-351964-hduv0ur4 172 22 . . . cord-351964-hduv0ur4 173 1 As as IN cord-351964-hduv0ur4 173 2 we -PRON- PRP cord-351964-hduv0ur4 173 3 have have VBP cord-351964-hduv0ur4 173 4 recently recently RB cord-351964-hduv0ur4 173 5 shown show VBN cord-351964-hduv0ur4 173 6 in in IN cord-351964-hduv0ur4 173 7 ART20 ART20 NNP cord-351964-hduv0ur4 173 8 cells cell NNS cord-351964-hduv0ur4 173 9 , , , cord-351964-hduv0ur4 173 10 detachment detachment NN cord-351964-hduv0ur4 173 11 of of IN cord-351964-hduv0ur4 173 12 secretory secretory JJ cord-351964-hduv0ur4 173 13 granules granule NNS cord-351964-hduv0ur4 173 14 from from IN cord-351964-hduv0ur4 173 15 the the DT cord-351964-hduv0ur4 173 16 trans trans NNP cord-351964-hduv0ur4 173 17 - - NNP cord-351964-hduv0ur4 173 18 Golgi Golgi NNP cord-351964-hduv0ur4 173 19 network network NN cord-351964-hduv0ur4 173 20 occurs occur VBZ cord-351964-hduv0ur4 173 21 at at IN cord-351964-hduv0ur4 173 22 a a DT cord-351964-hduv0ur4 173 23 late late JJ cord-351964-hduv0ur4 173 24 stage stage NN cord-351964-hduv0ur4 173 25 in in IN cord-351964-hduv0ur4 173 26 Tooze Tooze NNP cord-351964-hduv0ur4 173 27 et et NNP cord-351964-hduv0ur4 173 28 infection infection NN cord-351964-hduv0ur4 173 29 . . . cord-351964-hduv0ur4 174 1 Aft20 Aft20 NNP cord-351964-hduv0ur4 174 2 cells cell NNS cord-351964-hduv0ur4 174 3 were be VBD cord-351964-hduv0ur4 174 4 infected infect VBN cord-351964-hduv0ur4 174 5 with with IN cord-351964-hduv0ur4 174 6 MHV MHV NNP cord-351964-hduv0ur4 174 7 - - HYPH cord-351964-hduv0ur4 174 8 A59 A59 NNP cord-351964-hduv0ur4 174 9 for for IN cord-351964-hduv0ur4 174 10 4.5 4.5 CD cord-351964-hduv0ur4 174 11 h h NN cord-351964-hduv0ur4 174 12 and and CC cord-351964-hduv0ur4 174 13 then then RB cord-351964-hduv0ur4 174 14 with with IN cord-351964-hduv0ur4 174 15 VSV VSV NNP cord-351964-hduv0ur4 174 16 for for IN cord-351964-hduv0ur4 174 17 a a DT cord-351964-hduv0ur4 174 18 further further JJ cord-351964-hduv0ur4 174 19 3.5 3.5 CD cord-351964-hduv0ur4 174 20 h. h. NN cord-351964-hduv0ur4 175 1 After after IN cord-351964-hduv0ur4 175 2 infection infection NN cord-351964-hduv0ur4 175 3 with with IN cord-351964-hduv0ur4 175 4 VSG VSG NNP cord-351964-hduv0ur4 175 5 the the DT cord-351964-hduv0ur4 175 6 ceils ceil NNS cord-351964-hduv0ur4 175 7 were be VBD cord-351964-hduv0ur4 175 8 pulse pulse NN cord-351964-hduv0ur4 175 9 - - HYPH cord-351964-hduv0ur4 175 10 chased chase VBN cord-351964-hduv0ur4 175 11 with with IN cord-351964-hduv0ur4 175 12 [ [ -LRB- cord-351964-hduv0ur4 175 13 3SS]methionine 3SS]methionine NNP cord-351964-hduv0ur4 175 14 ( ( -LRB- cord-351964-hduv0ur4 175 15 see see VB cord-351964-hduv0ur4 175 16 Materials material NNS cord-351964-hduv0ur4 175 17 and and CC cord-351964-hduv0ur4 175 18 Methods Methods NNPS cord-351964-hduv0ur4 175 19 ) ) -RRB- cord-351964-hduv0ur4 175 20 and and CC cord-351964-hduv0ur4 175 21 the the DT cord-351964-hduv0ur4 175 22 G g NN cord-351964-hduv0ur4 175 23 protein protein NN cord-351964-hduv0ur4 175 24 of of IN cord-351964-hduv0ur4 175 25 VSV VSV NNP cord-351964-hduv0ur4 175 26 and and CC cord-351964-hduv0ur4 175 27 the the DT cord-351964-hduv0ur4 175 28 E1 e1 NN cord-351964-hduv0ur4 175 29 protein protein NN cord-351964-hduv0ur4 175 30 of of IN cord-351964-hduv0ur4 175 31 MHV MHV NNP cord-351964-hduv0ur4 175 32 - - HYPH cord-351964-hduv0ur4 175 33 A59 A59 NNP cord-351964-hduv0ur4 176 1 were be VBD cord-351964-hduv0ur4 176 2 then then RB cord-351964-hduv0ur4 176 3 enriched enrich VBN cord-351964-hduv0ur4 176 4 by by IN cord-351964-hduv0ur4 176 5 TX TX NNP cord-351964-hduv0ur4 176 6 - - HYPH cord-351964-hduv0ur4 176 7 U4 u4 NN cord-351964-hduv0ur4 176 8 phase phase NN cord-351964-hduv0ur4 176 9 partition partition NN cord-351964-hduv0ur4 176 10 as as IN cord-351964-hduv0ur4 176 11 described describe VBN cord-351964-hduv0ur4 176 12 in in IN cord-351964-hduv0ur4 176 13 Materials material NNS cord-351964-hduv0ur4 176 14 and and CC cord-351964-hduv0ur4 176 15 Methods method NNS cord-351964-hduv0ur4 176 16 . . . cord-351964-hduv0ur4 177 1 The the DT cord-351964-hduv0ur4 177 2 increase increase NN cord-351964-hduv0ur4 177 3 in in IN cord-351964-hduv0ur4 177 4 apparent apparent JJ cord-351964-hduv0ur4 177 5 molecular molecular JJ cord-351964-hduv0ur4 177 6 weight weight NN cord-351964-hduv0ur4 177 7 of of IN cord-351964-hduv0ur4 177 8 G g NN cord-351964-hduv0ur4 177 9 protein protein NN cord-351964-hduv0ur4 177 10 , , , cord-351964-hduv0ur4 177 11 which which WDT cord-351964-hduv0ur4 177 12 indicates indicate VBZ cord-351964-hduv0ur4 177 13 its -PRON- PRP$ cord-351964-hduv0ur4 177 14 sialylation sialylation NN cord-351964-hduv0ur4 177 15 , , , cord-351964-hduv0ur4 177 16 was be VBD cord-351964-hduv0ur4 177 17 first first RB cord-351964-hduv0ur4 177 18 detected detect VBN cord-351964-hduv0ur4 177 19 between between IN cord-351964-hduv0ur4 177 20 15 15 CD cord-351964-hduv0ur4 177 21 and and CC cord-351964-hduv0ur4 177 22 30 30 CD cord-351964-hduv0ur4 177 23 min min NN cord-351964-hduv0ur4 177 24 and and CC cord-351964-hduv0ur4 177 25 occurs occur VBZ cord-351964-hduv0ur4 177 26 with with IN cord-351964-hduv0ur4 177 27 the the DT cord-351964-hduv0ur4 177 28 same same JJ cord-351964-hduv0ur4 177 29 kinetics kinetic NNS cord-351964-hduv0ur4 177 30 in in IN cord-351964-hduv0ur4 177 31 the the DT cord-351964-hduv0ur4 177 32 presence presence NN cord-351964-hduv0ur4 177 33 or or CC cord-351964-hduv0ur4 177 34 absence absence NN cord-351964-hduv0ur4 177 35 of of IN cord-351964-hduv0ur4 177 36 MHV MHV NNP cord-351964-hduv0ur4 177 37 - - HYPH cord-351964-hduv0ur4 177 38 A59 A59 NNP cord-351964-hduv0ur4 177 39 infection infection NN cord-351964-hduv0ur4 177 40 . . . cord-351964-hduv0ur4 178 1 The the DT cord-351964-hduv0ur4 178 2 MHV MHV NNP cord-351964-hduv0ur4 178 3 - - HYPH cord-351964-hduv0ur4 178 4 A59 A59 NNP cord-351964-hduv0ur4 178 5 E1 e1 NN cord-351964-hduv0ur4 178 6 protein protein NN cord-351964-hduv0ur4 178 7 is be VBZ cord-351964-hduv0ur4 178 8 seen see VBN cord-351964-hduv0ur4 178 9 in in IN cord-351964-hduv0ur4 178 10 the the DT cord-351964-hduv0ur4 178 11 infected infected JJ cord-351964-hduv0ur4 178 12 cells cell NNS cord-351964-hduv0ur4 178 13 ( ( -LRB- cord-351964-hduv0ur4 178 14 + + CC cord-351964-hduv0ur4 178 15 lanes lane NNS cord-351964-hduv0ur4 178 16 ) ) -RRB- cord-351964-hduv0ur4 178 17 in in IN cord-351964-hduv0ur4 178 18 a a DT cord-351964-hduv0ur4 178 19 longer long JJR cord-351964-hduv0ur4 178 20 exposure exposure NN cord-351964-hduv0ur4 178 21 of of IN cord-351964-hduv0ur4 178 22 the the DT cord-351964-hduv0ur4 178 23 bottom bottom JJ cord-351964-hduv0ur4 178 24 portion portion NN cord-351964-hduv0ur4 178 25 of of IN cord-351964-hduv0ur4 178 26 the the DT cord-351964-hduv0ur4 178 27 gel gel NN cord-351964-hduv0ur4 178 28 . . . cord-351964-hduv0ur4 179 1 The the DT cord-351964-hduv0ur4 179 2 E1 e1 NN cord-351964-hduv0ur4 179 3 is be VBZ cord-351964-hduv0ur4 179 4 the the DT cord-351964-hduv0ur4 179 5 middle middle JJ cord-351964-hduv0ur4 179 6 band band NN cord-351964-hduv0ur4 179 7 of of IN cord-351964-hduv0ur4 179 8 the the DT cord-351964-hduv0ur4 179 9 three three CD cord-351964-hduv0ur4 179 10 bands band NNS cord-351964-hduv0ur4 179 11 within within IN cord-351964-hduv0ur4 179 12 the the DT cord-351964-hduv0ur4 179 13 bracket bracket NN cord-351964-hduv0ur4 179 14 . . . cord-351964-hduv0ur4 180 1 This this DT cord-351964-hduv0ur4 180 2 establishes establish VBZ cord-351964-hduv0ur4 180 3 that that IN cord-351964-hduv0ur4 180 4 the the DT cord-351964-hduv0ur4 180 5 E1 E1 NNP cord-351964-hduv0ur4 180 6 protein protein NN cord-351964-hduv0ur4 180 7 of of IN cord-351964-hduv0ur4 180 8 MHV MHV NNP cord-351964-hduv0ur4 180 9 - - HYPH cord-351964-hduv0ur4 180 10 A59 A59 NNP cord-351964-hduv0ur4 180 11 was be VBD cord-351964-hduv0ur4 180 12 being be VBG cord-351964-hduv0ur4 180 13 synthesized synthesize VBN cord-351964-hduv0ur4 180 14 at at IN cord-351964-hduv0ur4 180 15 the the DT cord-351964-hduv0ur4 180 16 same same JJ cord-351964-hduv0ur4 180 17 time time NN cord-351964-hduv0ur4 180 18 as as IN cord-351964-hduv0ur4 180 19 the the DT cord-351964-hduv0ur4 180 20 VSV VSV NNP cord-351964-hduv0ur4 180 21 G G NNP cord-351964-hduv0ur4 180 22 protein protein NN cord-351964-hduv0ur4 180 23 . . . cord-351964-hduv0ur4 181 1 the the DT cord-351964-hduv0ur4 181 2 maturation maturation NN cord-351964-hduv0ur4 181 3 of of IN cord-351964-hduv0ur4 181 4 the the DT cord-351964-hduv0ur4 181 5 cores core NNS cord-351964-hduv0ur4 181 6 , , , cord-351964-hduv0ur4 181 7 after after IN cord-351964-hduv0ur4 181 8 they -PRON- PRP cord-351964-hduv0ur4 181 9 have have VBP cord-351964-hduv0ur4 181 10 become become VBN cord-351964-hduv0ur4 181 11 condensed condense VBN cord-351964-hduv0ur4 181 12 and and CC cord-351964-hduv0ur4 181 13 have have VBP cord-351964-hduv0ur4 181 14 assumed assume VBN cord-351964-hduv0ur4 181 15 a a DT cord-351964-hduv0ur4 181 16 spherical spherical JJ cord-351964-hduv0ur4 181 17 or or CC cord-351964-hduv0ur4 181 18 ovoid ovoid JJ cord-351964-hduv0ur4 181 19 shape shape NN cord-351964-hduv0ur4 181 20 with with IN cord-351964-hduv0ur4 181 21 a a DT cord-351964-hduv0ur4 181 22 well well RB cord-351964-hduv0ur4 181 23 - - HYPH cord-351964-hduv0ur4 181 24 defined define VBN cord-351964-hduv0ur4 181 25 surface surface NN cord-351964-hduv0ur4 181 26 . . . cord-351964-hduv0ur4 182 1 The the DT cord-351964-hduv0ur4 182 2 two two CD cord-351964-hduv0ur4 182 3 cores core NNS cord-351964-hduv0ur4 182 4 shown show VBN cord-351964-hduv0ur4 182 5 in in IN cord-351964-hduv0ur4 182 6 Fig Fig NNP cord-351964-hduv0ur4 182 7 . . . cord-351964-hduv0ur4 183 1 7 7 CD cord-351964-hduv0ur4 183 2 B b NN cord-351964-hduv0ur4 183 3 , , , cord-351964-hduv0ur4 183 4 inset inset NN cord-351964-hduv0ur4 183 5 are be VBP cord-351964-hduv0ur4 183 6 at at IN cord-351964-hduv0ur4 183 7 a a DT cord-351964-hduv0ur4 183 8 stage stage NN cord-351964-hduv0ur4 183 9 at at IN cord-351964-hduv0ur4 183 10 which which WDT cord-351964-hduv0ur4 183 11 they -PRON- PRP cord-351964-hduv0ur4 183 12 detach detach VBP cord-351964-hduv0ur4 183 13 from from IN cord-351964-hduv0ur4 183 14 the the DT cord-351964-hduv0ur4 183 15 trans trans NNP cord-351964-hduv0ur4 183 16 - - NNP cord-351964-hduv0ur4 183 17 Golgi Golgi NNP cord-351964-hduv0ur4 183 18 network network NN cord-351964-hduv0ur4 183 19 . . . cord-351964-hduv0ur4 184 1 Note note VB cord-351964-hduv0ur4 184 2 also also RB cord-351964-hduv0ur4 184 3 that that IN cord-351964-hduv0ur4 184 4 there there EX cord-351964-hduv0ur4 184 5 are be VBP cord-351964-hduv0ur4 184 6 no no DT cord-351964-hduv0ur4 184 7 virions virion NNS cord-351964-hduv0ur4 184 8 in in IN cord-351964-hduv0ur4 184 9 the the DT cord-351964-hduv0ur4 184 10 secretory secretory JJ cord-351964-hduv0ur4 184 11 granule granule NN cord-351964-hduv0ur4 184 12 close close RB cord-351964-hduv0ur4 184 13 to to IN cord-351964-hduv0ur4 184 14 the the DT cord-351964-hduv0ur4 184 15 Golgi Golgi NNP cord-351964-hduv0ur4 184 16 stack stack NN cord-351964-hduv0ur4 184 17 shown show VBN cord-351964-hduv0ur4 184 18 in in IN cord-351964-hduv0ur4 184 19 Fig Fig NNP cord-351964-hduv0ur4 184 20 . . . cord-351964-hduv0ur4 185 1 1 1 LS cord-351964-hduv0ur4 185 2 . . . cord-351964-hduv0ur4 186 1 We -PRON- PRP cord-351964-hduv0ur4 186 2 have have VBP cord-351964-hduv0ur4 186 3 never never RB cord-351964-hduv0ur4 186 4 seen see VBN cord-351964-hduv0ur4 186 5 virions virion NNS cord-351964-hduv0ur4 186 6 embedded embed VBN cord-351964-hduv0ur4 186 7 in in IN cord-351964-hduv0ur4 186 8 secretory secretory JJ cord-351964-hduv0ur4 186 9 granule granule NN cord-351964-hduv0ur4 186 10 cores core NNS cord-351964-hduv0ur4 186 11 either either CC cord-351964-hduv0ur4 186 12 in in IN cord-351964-hduv0ur4 186 13 the the DT cord-351964-hduv0ur4 186 14 Golgi Golgi NNP cord-351964-hduv0ur4 186 15 region region NN cord-351964-hduv0ur4 186 16 or or CC cord-351964-hduv0ur4 186 17 at at IN cord-351964-hduv0ur4 186 18 the the DT cord-351964-hduv0ur4 186 19 cell cell NN cord-351964-hduv0ur4 186 20 periphery periphery NN cord-351964-hduv0ur4 186 21 , , , cord-351964-hduv0ur4 186 22 where where WRB cord-351964-hduv0ur4 186 23 the the DT cord-351964-hduv0ur4 186 24 mature mature JJ cord-351964-hduv0ur4 186 25 granules granule NNS cord-351964-hduv0ur4 186 26 accumulate accumulate VBP cord-351964-hduv0ur4 186 27 . . . cord-351964-hduv0ur4 187 1 Fig Fig NNP cord-351964-hduv0ur4 187 2 . . . cord-351964-hduv0ur4 188 1 8 8 CD cord-351964-hduv0ur4 188 2 shows show NNS cord-351964-hduv0ur4 188 3 condensing condense VBG cord-351964-hduv0ur4 188 4 secretory secretory JJ cord-351964-hduv0ur4 188 5 protein protein NN cord-351964-hduv0ur4 188 6 in in IN cord-351964-hduv0ur4 188 7 the the DT cord-351964-hduv0ur4 188 8 trans trans NNP cord-351964-hduv0ur4 188 9 - - NNP cord-351964-hduv0ur4 188 10 Golgi Golgi NNP cord-351964-hduv0ur4 188 11 network network NN cord-351964-hduv0ur4 188 12 of of IN cord-351964-hduv0ur4 188 13 uninfected uninfected JJ cord-351964-hduv0ur4 188 14 AtT20 AtT20 NNP cord-351964-hduv0ur4 188 15 cells cell NNS cord-351964-hduv0ur4 188 16 . . . cord-351964-hduv0ur4 189 1 Comparison comparison NN cord-351964-hduv0ur4 189 2 of of IN cord-351964-hduv0ur4 189 3 Fig Fig NNP cord-351964-hduv0ur4 189 4 . . . cord-351964-hduv0ur4 190 1 7 7 LS cord-351964-hduv0ur4 190 2 , , , cord-351964-hduv0ur4 190 3 A a NN cord-351964-hduv0ur4 190 4 and and CC cord-351964-hduv0ur4 190 5 B b NN cord-351964-hduv0ur4 190 6 with with IN cord-351964-hduv0ur4 190 7 Fig Fig NNP cord-351964-hduv0ur4 190 8 . . . cord-351964-hduv0ur4 191 1 8 8 CD cord-351964-hduv0ur4 191 2 shows show VBZ cord-351964-hduv0ur4 191 3 that that IN cord-351964-hduv0ur4 191 4 the the DT cord-351964-hduv0ur4 191 5 only only JJ cord-351964-hduv0ur4 191 6 detectable detectable JJ cord-351964-hduv0ur4 191 7 change change NN cord-351964-hduv0ur4 191 8 at at IN cord-351964-hduv0ur4 191 9 these these DT cord-351964-hduv0ur4 191 10 early early JJ cord-351964-hduv0ur4 191 11 times time NNS cord-351964-hduv0ur4 191 12 after after IN cord-351964-hduv0ur4 191 13 infection infection NN cord-351964-hduv0ur4 191 14 with with IN cord-351964-hduv0ur4 191 15 MHV MHV NNP cord-351964-hduv0ur4 191 16 - - HYPH cord-351964-hduv0ur4 191 17 A59 A59 NNP cord-351964-hduv0ur4 191 18 is be VBZ cord-351964-hduv0ur4 191 19 the the DT cord-351964-hduv0ur4 191 20 presence presence NN cord-351964-hduv0ur4 191 21 of of IN cord-351964-hduv0ur4 191 22 virions virion NNS cord-351964-hduv0ur4 191 23 in in IN cord-351964-hduv0ur4 191 24 the the DT cord-351964-hduv0ur4 191 25 Golgi Golgi NNP cord-351964-hduv0ur4 191 26 complex complex NN cord-351964-hduv0ur4 191 27 . . . cord-351964-hduv0ur4 192 1 Clathrin Clathrin NNP cord-351964-hduv0ur4 192 2 is be VBZ cord-351964-hduv0ur4 192 3 a a DT cord-351964-hduv0ur4 192 4 marker marker NN cord-351964-hduv0ur4 192 5 for for IN cord-351964-hduv0ur4 192 6 the the DT cord-351964-hduv0ur4 192 7 trans trans JJ cord-351964-hduv0ur4 192 8 - - NN cord-351964-hduv0ur4 192 9 side side NN cord-351964-hduv0ur4 192 10 of of IN cord-351964-hduv0ur4 192 11 the the DT cord-351964-hduv0ur4 192 12 Golgi Golgi NNP cord-351964-hduv0ur4 192 13 apparatus apparatus NN cord-351964-hduv0ur4 192 14 ( ( -LRB- cord-351964-hduv0ur4 192 15 for for IN cord-351964-hduv0ur4 192 16 review review NN cord-351964-hduv0ur4 192 17 see see VBP cord-351964-hduv0ur4 192 18 Griffiths Griffiths NNP cord-351964-hduv0ur4 192 19 and and CC cord-351964-hduv0ur4 192 20 Simons Simons NNP cord-351964-hduv0ur4 192 21 , , , cord-351964-hduv0ur4 192 22 1986 1986 CD cord-351964-hduv0ur4 192 23 ) ) -RRB- cord-351964-hduv0ur4 192 24 and and CC cord-351964-hduv0ur4 192 25 , , , cord-351964-hduv0ur4 192 26 as as IN cord-351964-hduv0ur4 192 27 we -PRON- PRP cord-351964-hduv0ur4 192 28 have have VBP cord-351964-hduv0ur4 192 29 previously previously RB cord-351964-hduv0ur4 192 30 shown show VBN cord-351964-hduv0ur4 192 31 by by IN cord-351964-hduv0ur4 192 32 inununocytochemistry inununocytochemistry NNP cord-351964-hduv0ur4 192 33 , , , cord-351964-hduv0ur4 192 34 in in IN cord-351964-hduv0ur4 192 35 uninfected uninfected JJ cord-351964-hduv0ur4 192 36 AtT20 AtT20 NNP cord-351964-hduv0ur4 192 37 ceils ceil NNS cord-351964-hduv0ur4 193 1 the the DT cord-351964-hduv0ur4 193 2 trans trans NNP cord-351964-hduv0ur4 193 3 - - NNP cord-351964-hduv0ur4 193 4 Golgi Golgi NNP cord-351964-hduv0ur4 193 5 network network NN cord-351964-hduv0ur4 193 6 and and CC cord-351964-hduv0ur4 193 7 immature immature JJ cord-351964-hduv0ur4 193 8 secretory secretory JJ cord-351964-hduv0ur4 193 9 granules granule NNS cord-351964-hduv0ur4 193 10 in in IN cord-351964-hduv0ur4 193 11 the the DT cord-351964-hduv0ur4 193 12 Golgi Golgi NNP cord-351964-hduv0ur4 193 13 region region NN cord-351964-hduv0ur4 193 14 often often RB cord-351964-hduv0ur4 193 15 have have VBP cord-351964-hduv0ur4 193 16 clathrin clathrin NN cord-351964-hduv0ur4 193 17 coats coat NNS cord-351964-hduv0ur4 193 18 on on IN cord-351964-hduv0ur4 193 19 parts part NNS cord-351964-hduv0ur4 193 20 of of IN cord-351964-hduv0ur4 193 21 their -PRON- PRP$ cord-351964-hduv0ur4 193 22 surface surface NN cord-351964-hduv0ur4 193 23 ( ( -LRB- cord-351964-hduv0ur4 193 24 Fig Fig NNP cord-351964-hduv0ur4 193 25 . . . cord-351964-hduv0ur4 193 26 8) 8) NNP cord-351964-hduv0ur4 193 27 , , , cord-351964-hduv0ur4 193 28 and and CC cord-351964-hduv0ur4 193 29 are be VBP cord-351964-hduv0ur4 193 30 the the DT cord-351964-hduv0ur4 193 31 site site NN cord-351964-hduv0ur4 193 32 of of IN cord-351964-hduv0ur4 193 33 budding bud VBG cord-351964-hduv0ur4 193 34 or or CC cord-351964-hduv0ur4 193 35 fusion fusion NN cord-351964-hduv0ur4 193 36 of of IN cord-351964-hduv0ur4 193 37 clathrin clathrin NN cord-351964-hduv0ur4 193 38 - - HYPH cord-351964-hduv0ur4 193 39 coated coat VBN cord-351964-hduv0ur4 193 40 vesicles vesicle NNS cord-351964-hduv0ur4 193 41 . . . cord-351964-hduv0ur4 194 1 Coats coat NNS cord-351964-hduv0ur4 194 2 that that WDT cord-351964-hduv0ur4 194 3 resemble resemble VBP cord-351964-hduv0ur4 194 4 clathrin clathrin NN cord-351964-hduv0ur4 194 5 in in IN cord-351964-hduv0ur4 194 6 morphology morphology NN cord-351964-hduv0ur4 194 7 , , , cord-351964-hduv0ur4 194 8 but but CC cord-351964-hduv0ur4 194 9 were be VBD cord-351964-hduv0ur4 194 10 not not RB cord-351964-hduv0ur4 194 11 positively positively RB cord-351964-hduv0ur4 194 12 identified identify VBN cord-351964-hduv0ur4 194 13 as as IN cord-351964-hduv0ur4 194 14 such such JJ cord-351964-hduv0ur4 194 15 by by IN cord-351964-hduv0ur4 194 16 immunocytochemistry immunocytochemistry NNP cord-351964-hduv0ur4 194 17 , , , cord-351964-hduv0ur4 194 18 were be VBD cord-351964-hduv0ur4 194 19 also also RB cord-351964-hduv0ur4 194 20 often often RB cord-351964-hduv0ur4 194 21 present present JJ cord-351964-hduv0ur4 194 22 in in IN cord-351964-hduv0ur4 194 23 infected infected JJ cord-351964-hduv0ur4 194 24 cells cell NNS cord-351964-hduv0ur4 194 25 on on IN cord-351964-hduv0ur4 194 26 the the DT cord-351964-hduv0ur4 194 27 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 194 28 face face NN cord-351964-hduv0ur4 194 29 of of IN cord-351964-hduv0ur4 194 30 parts part NNS cord-351964-hduv0ur4 194 31 of of IN cord-351964-hduv0ur4 194 32 the the DT cord-351964-hduv0ur4 194 33 trans trans NNP cord-351964-hduv0ur4 194 34 - - NNP cord-351964-hduv0ur4 194 35 Golgi Golgi NNP cord-351964-hduv0ur4 194 36 network network NN cord-351964-hduv0ur4 194 37 containing contain VBG cord-351964-hduv0ur4 194 38 virions virion NNS cord-351964-hduv0ur4 194 39 and and CC cord-351964-hduv0ur4 194 40 on on IN cord-351964-hduv0ur4 194 41 peri peri NNP cord-351964-hduv0ur4 194 42 - - HYPH cord-351964-hduv0ur4 194 43 Golgi golgi NN cord-351964-hduv0ur4 194 44 vesicles vesicle NNS cord-351964-hduv0ur4 194 45 with with IN cord-351964-hduv0ur4 194 46 virions virion NNS cord-351964-hduv0ur4 194 47 ( ( -LRB- cord-351964-hduv0ur4 194 48 Figs fig NNS cord-351964-hduv0ur4 194 49 . . . cord-351964-hduv0ur4 195 1 7 7 CD cord-351964-hduv0ur4 195 2 A a NN cord-351964-hduv0ur4 195 3 and and CC cord-351964-hduv0ur4 195 4 9 9 CD cord-351964-hduv0ur4 195 5 ) ) -RRB- cord-351964-hduv0ur4 195 6 . . . cord-351964-hduv0ur4 196 1 The the DT cord-351964-hduv0ur4 196 2 observations observation NNS cord-351964-hduv0ur4 196 3 presented present VBN cord-351964-hduv0ur4 196 4 above above IN cord-351964-hduv0ur4 196 5 indicate indicate VBP cord-351964-hduv0ur4 196 6 : : : cord-351964-hduv0ur4 196 7 ( ( -LRB- cord-351964-hduv0ur4 196 8 a a LS cord-351964-hduv0ur4 196 9 ) ) -RRB- cord-351964-hduv0ur4 196 10 That that IN cord-351964-hduv0ur4 196 11 virions virion NNS cord-351964-hduv0ur4 196 12 and and CC cord-351964-hduv0ur4 196 13 secretory secretory NN cord-351964-hduv0ur4 196 14 proteins protein NNS cord-351964-hduv0ur4 196 15 traverse traverse VBP cord-351964-hduv0ur4 196 16 the the DT cord-351964-hduv0ur4 196 17 same same JJ cord-351964-hduv0ur4 196 18 Golgi Golgi NNP cord-351964-hduv0ur4 196 19 stacks stack NNS cord-351964-hduv0ur4 196 20 and and CC cord-351964-hduv0ur4 196 21 accumulate accumulate VBP cord-351964-hduv0ur4 196 22 in in IN cord-351964-hduv0ur4 196 23 the the DT cord-351964-hduv0ur4 196 24 trans trans NNP cord-351964-hduv0ur4 196 25 - - NNP cord-351964-hduv0ur4 196 26 Golgi Golgi NNP cord-351964-hduv0ur4 196 27 network network NN cord-351964-hduv0ur4 196 28 ; ; : cord-351964-hduv0ur4 196 29 we -PRON- PRP cord-351964-hduv0ur4 196 30 have have VBP cord-351964-hduv0ur4 196 31 shown show VBN cord-351964-hduv0ur4 196 32 previously previously RB cord-351964-hduv0ur4 196 33 by by IN cord-351964-hduv0ur4 196 34 immunocytochemistry immunocytochemistry NN cord-351964-hduv0ur4 196 35 that that IN cord-351964-hduv0ur4 196 36 ACTH ACTH NNP cord-351964-hduv0ur4 196 37 is be VBZ cord-351964-hduv0ur4 196 38 present present JJ cord-351964-hduv0ur4 196 39 in in IN cord-351964-hduv0ur4 196 40 all all DT cord-351964-hduv0ur4 196 41 cisternae cisternae NN cord-351964-hduv0ur4 196 42 of of IN cord-351964-hduv0ur4 196 43 the the DT cord-351964-hduv0ur4 196 44 Golgi Golgi NNP cord-351964-hduv0ur4 196 45 stacks stack NNS cord-351964-hduv0ur4 196 46 of of IN cord-351964-hduv0ur4 196 47 AtT20 AtT20 NNP cord-351964-hduv0ur4 196 48 cells cell NNS cord-351964-hduv0ur4 196 49 ( ( -LRB- cord-351964-hduv0ur4 196 50 Tooze Tooze NNP cord-351964-hduv0ur4 196 51 and and CC cord-351964-hduv0ur4 196 52 Tooze Tooze NNP cord-351964-hduv0ur4 196 53 , , , cord-351964-hduv0ur4 196 54 Note note VB cord-351964-hduv0ur4 196 55 that that IN cord-351964-hduv0ur4 196 56 only only RB cord-351964-hduv0ur4 196 57 morphologically morphologically RB cord-351964-hduv0ur4 196 58 mature mature JJ cord-351964-hduv0ur4 196 59 virions virion NNS cord-351964-hduv0ur4 196 60 are be VBP cord-351964-hduv0ur4 196 61 found find VBN cord-351964-hduv0ur4 196 62 on on IN cord-351964-hduv0ur4 196 63 the the DT cord-351964-hduv0ur4 196 64 trans trans JJ cord-351964-hduv0ur4 196 65 - - NN cord-351964-hduv0ur4 196 66 side side NN cord-351964-hduv0ur4 196 67 of of IN cord-351964-hduv0ur4 196 68 the the DT cord-351964-hduv0ur4 196 69 Golgi Golgi NNP cord-351964-hduv0ur4 196 70 complex complex NN cord-351964-hduv0ur4 196 71 . . . cord-351964-hduv0ur4 197 1 Many many JJ cord-351964-hduv0ur4 197 2 dilations dilation NNS cord-351964-hduv0ur4 197 3 of of IN cord-351964-hduv0ur4 197 4 the the DT cord-351964-hduv0ur4 197 5 trans trans NNP cord-351964-hduv0ur4 197 6 - - NNP cord-351964-hduv0ur4 197 7 Golgi Golgi NNP cord-351964-hduv0ur4 197 8 network network NN cord-351964-hduv0ur4 197 9 contain contain VBP cord-351964-hduv0ur4 197 10 either either CC cord-351964-hduv0ur4 197 11 condensing condense VBG cord-351964-hduv0ur4 197 12 secretory secretory NN cord-351964-hduv0ur4 197 13 proteins protein NNS cord-351964-hduv0ur4 197 14 ( ( -LRB- cord-351964-hduv0ur4 197 15 arrowheads arrowhead NNS cord-351964-hduv0ur4 197 16 ) ) -RRB- cord-351964-hduv0ur4 197 17 or or CC cord-351964-hduv0ur4 197 18 condensed condense VBN cord-351964-hduv0ur4 197 19 virions virion NNS cord-351964-hduv0ur4 197 20 ( ( -LRB- cord-351964-hduv0ur4 197 21 /arge /arge JJ cord-351964-hduv0ur4 197 22 arrows arrow NNS cord-351964-hduv0ur4 197 23 ) ) -RRB- cord-351964-hduv0ur4 197 24 . . . cord-351964-hduv0ur4 198 1 When when WRB cord-351964-hduv0ur4 198 2 both both DT cord-351964-hduv0ur4 198 3 virions virion NNS cord-351964-hduv0ur4 198 4 and and CC cord-351964-hduv0ur4 198 5 condensing condense VBG cord-351964-hduv0ur4 198 6 secretory secretory JJ cord-351964-hduv0ur4 198 7 proteins protein NNS cord-351964-hduv0ur4 198 8 share share VBP cord-351964-hduv0ur4 198 9 the the DT cord-351964-hduv0ur4 198 10 same same JJ cord-351964-hduv0ur4 198 11 dilation dilation NN cord-351964-hduv0ur4 198 12 , , , cord-351964-hduv0ur4 198 13 they -PRON- PRP cord-351964-hduv0ur4 198 14 are be VBP cord-351964-hduv0ur4 198 15 well well RB cord-351964-hduv0ur4 198 16 separated separate VBN cord-351964-hduv0ur4 198 17 ( ( -LRB- cord-351964-hduv0ur4 198 18 lower low JJR cord-351964-hduv0ur4 198 19 leJ lej NN cord-351964-hduv0ur4 198 20 ? ? . cord-351964-hduv0ur4 198 21 and and CC cord-351964-hduv0ur4 198 22 inset inset NN cord-351964-hduv0ur4 198 23 ) ) -RRB- cord-351964-hduv0ur4 198 24 . . . cord-351964-hduv0ur4 199 1 We -PRON- PRP cord-351964-hduv0ur4 199 2 have have VBP cord-351964-hduv0ur4 199 3 previously previously RB cord-351964-hduv0ur4 199 4 shown show VBN cord-351964-hduv0ur4 199 5 that that IN cord-351964-hduv0ur4 199 6 the the DT cord-351964-hduv0ur4 199 7 coat coat NN cord-351964-hduv0ur4 199 8 on on IN cord-351964-hduv0ur4 199 9 the the DT cord-351964-hduv0ur4 199 10 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 199 11 surface surface NN cord-351964-hduv0ur4 199 12 of of IN cord-351964-hduv0ur4 199 13 the the DT cord-351964-hduv0ur4 199 14 trans trans NNP cord-351964-hduv0ur4 199 15 - - NNP cord-351964-hduv0ur4 199 16 Golgi Golgi NNP cord-351964-hduv0ur4 199 17 network network NN cord-351964-hduv0ur4 199 18 ( ( -LRB- cord-351964-hduv0ur4 199 19 between between IN cord-351964-hduv0ur4 199 20 the the DT cord-351964-hduv0ur4 199 21 small small JJ cord-351964-hduv0ur4 199 22 arrows arrow NNS cord-351964-hduv0ur4 199 23 ) ) -RRB- cord-351964-hduv0ur4 199 24 is be VBZ cord-351964-hduv0ur4 199 25 clathrin clathrin NNP cord-351964-hduv0ur4 199 26 . . . cord-351964-hduv0ur4 200 1 Note note VB cord-351964-hduv0ur4 200 2 the the DT cord-351964-hduv0ur4 200 3 coated coated JJ cord-351964-hduv0ur4 200 4 vesicle vesicle NN cord-351964-hduv0ur4 200 5 budding bud VBG cord-351964-hduv0ur4 200 6 from from IN cord-351964-hduv0ur4 200 7 or or CC cord-351964-hduv0ur4 200 8 fusing fuse VBG cord-351964-hduv0ur4 200 9 with with IN cord-351964-hduv0ur4 200 10 the the DT cord-351964-hduv0ur4 200 11 trans trans NNP cord-351964-hduv0ur4 200 12 - - NNP cord-351964-hduv0ur4 200 13 Golgi Golgi NNP cord-351964-hduv0ur4 200 14 network network NN cord-351964-hduv0ur4 200 15 ( ( -LRB- cord-351964-hduv0ur4 200 16 curved curved JJ cord-351964-hduv0ur4 200 17 arrow arrow NN cord-351964-hduv0ur4 200 18 ) ) -RRB- cord-351964-hduv0ur4 200 19 . . . cord-351964-hduv0ur4 201 1 ( ( -LRB- cord-351964-hduv0ur4 201 2 B b NN cord-351964-hduv0ur4 201 3 ) ) -RRB- cord-351964-hduv0ur4 202 1 In in IN cord-351964-hduv0ur4 202 2 this this DT cord-351964-hduv0ur4 202 3 example example NN cord-351964-hduv0ur4 202 4 the the DT cord-351964-hduv0ur4 202 5 trans trans NNP cord-351964-hduv0ur4 202 6 - - NNP cord-351964-hduv0ur4 202 7 Golgi Golgi NNP cord-351964-hduv0ur4 202 8 network network NN cord-351964-hduv0ur4 202 9 contains contain VBZ cord-351964-hduv0ur4 202 10 condensing condense VBG cord-351964-hduv0ur4 202 11 secretory secretory JJ cord-351964-hduv0ur4 202 12 protein protein NN cord-351964-hduv0ur4 202 13 ( ( -LRB- cord-351964-hduv0ur4 202 14 arrows arrow NNS cord-351964-hduv0ur4 202 15 ) ) -RRB- cord-351964-hduv0ur4 202 16 and and CC cord-351964-hduv0ur4 202 17 one one CD cord-351964-hduv0ur4 202 18 dilation dilation NN cord-351964-hduv0ur4 202 19 also also RB cord-351964-hduv0ur4 202 20 contains contain VBZ cord-351964-hduv0ur4 202 21 virions virion NNS cord-351964-hduv0ur4 202 22 ( ( -LRB- cord-351964-hduv0ur4 202 23 open open JJ cord-351964-hduv0ur4 202 24 arrow arrow NN cord-351964-hduv0ur4 202 25 ) ) -RRB- cord-351964-hduv0ur4 202 26 . . . cord-351964-hduv0ur4 203 1 The the DT cord-351964-hduv0ur4 203 2 virions virion NNS cord-351964-hduv0ur4 203 3 have have VBP cord-351964-hduv0ur4 203 4 the the DT cord-351964-hduv0ur4 203 5 mature mature JJ cord-351964-hduv0ur4 203 6 morphology morphology NN cord-351964-hduv0ur4 203 7 and and CC cord-351964-hduv0ur4 203 8 are be VBP cord-351964-hduv0ur4 203 9 well well RB cord-351964-hduv0ur4 203 10 separated separate VBN cord-351964-hduv0ur4 203 11 from from IN cord-351964-hduv0ur4 203 12 the the DT cord-351964-hduv0ur4 203 13 condensing condense VBG cord-351964-hduv0ur4 203 14 secretory secretory JJ cord-351964-hduv0ur4 203 15 protein protein NN cord-351964-hduv0ur4 203 16 . . . cord-351964-hduv0ur4 204 1 Note note VB cord-351964-hduv0ur4 204 2 that that IN cord-351964-hduv0ur4 204 3 the the DT cord-351964-hduv0ur4 204 4 latter latter NN cord-351964-hduv0ur4 204 5 is be VBZ cord-351964-hduv0ur4 204 6 more more RBR cord-351964-hduv0ur4 204 7 uniformly uniformly RB cord-351964-hduv0ur4 204 8 compact compact JJ cord-351964-hduv0ur4 204 9 than than IN cord-351964-hduv0ur4 204 10 in in IN cord-351964-hduv0ur4 204 11 A A NNP cord-351964-hduv0ur4 204 12 , , , cord-351964-hduv0ur4 204 13 and and CC cord-351964-hduv0ur4 204 14 is be VBZ cord-351964-hduv0ur4 204 15 beginning begin VBG cord-351964-hduv0ur4 204 16 to to TO cord-351964-hduv0ur4 204 17 assume assume VB cord-351964-hduv0ur4 204 18 the the DT cord-351964-hduv0ur4 204 19 form form NN cord-351964-hduv0ur4 204 20 of of IN cord-351964-hduv0ur4 204 21 a a DT cord-351964-hduv0ur4 204 22 secretory secretory JJ cord-351964-hduv0ur4 204 23 granule granule NN cord-351964-hduv0ur4 204 24 core core NN cord-351964-hduv0ur4 204 25 . . . cord-351964-hduv0ur4 205 1 ( ( -LRB- cord-351964-hduv0ur4 205 2 Insert insert VB cord-351964-hduv0ur4 205 3 ) ) -RRB- cord-351964-hduv0ur4 205 4 Part part NN cord-351964-hduv0ur4 205 5 of of IN cord-351964-hduv0ur4 205 6 the the DT cord-351964-hduv0ur4 205 7 trans trans NNP cord-351964-hduv0ur4 205 8 - - NNP cord-351964-hduv0ur4 205 9 Golgi Golgi NNP cord-351964-hduv0ur4 205 10 network network NN cord-351964-hduv0ur4 205 11 in in IN cord-351964-hduv0ur4 205 12 another another DT cord-351964-hduv0ur4 205 13 infected infected JJ cord-351964-hduv0ur4 205 14 cell cell NN cord-351964-hduv0ur4 205 15 in in IN cord-351964-hduv0ur4 205 16 which which WDT cord-351964-hduv0ur4 205 17 two two CD cord-351964-hduv0ur4 205 18 mature mature JJ cord-351964-hduv0ur4 205 19 virions virion NNS cord-351964-hduv0ur4 205 20 ( ( -LRB- cord-351964-hduv0ur4 205 21 arrow arrow NN cord-351964-hduv0ur4 205 22 ) ) -RRB- cord-351964-hduv0ur4 205 23 and and CC cord-351964-hduv0ur4 205 24 two two CD cord-351964-hduv0ur4 205 25 aggregates aggregate NNS cord-351964-hduv0ur4 205 26 of of IN cord-351964-hduv0ur4 205 27 secretory secretory JJ cord-351964-hduv0ur4 205 28 proteins protein NNS cord-351964-hduv0ur4 205 29 that that WDT cord-351964-hduv0ur4 205 30 have have VBP cord-351964-hduv0ur4 205 31 condensed condense VBN cord-351964-hduv0ur4 205 32 into into IN cord-351964-hduv0ur4 205 33 ovoid ovoid JJ cord-351964-hduv0ur4 205 34 granule granule NN cord-351964-hduv0ur4 205 35 core core NN cord-351964-hduv0ur4 205 36 structures structure NNS cord-351964-hduv0ur4 205 37 ( ( -LRB- cord-351964-hduv0ur4 205 38 arrowheads arrowhead NNS cord-351964-hduv0ur4 205 39 ) ) -RRB- cord-351964-hduv0ur4 205 40 are be VBP cord-351964-hduv0ur4 205 41 in in IN cord-351964-hduv0ur4 205 42 the the DT cord-351964-hduv0ur4 205 43 same same JJ cord-351964-hduv0ur4 205 44 compartment compartment NN cord-351964-hduv0ur4 205 45 but but CC cord-351964-hduv0ur4 205 46 still still RB cord-351964-hduv0ur4 205 47 quite quite RB cord-351964-hduv0ur4 205 48 separate separate JJ cord-351964-hduv0ur4 205 49 in in IN cord-351964-hduv0ur4 205 50 the the DT cord-351964-hduv0ur4 205 51 sense sense NN cord-351964-hduv0ur4 205 52 that that IN cord-351964-hduv0ur4 205 53 there there EX cord-351964-hduv0ur4 205 54 are be VBP cord-351964-hduv0ur4 205 55 no no DT cord-351964-hduv0ur4 205 56 virions virion NNS cord-351964-hduv0ur4 205 57 within within IN cord-351964-hduv0ur4 205 58 the the DT cord-351964-hduv0ur4 205 59 cores core NNS cord-351964-hduv0ur4 205 60 . . . cord-351964-hduv0ur4 206 1 Bars bar NNS cord-351964-hduv0ur4 206 2 , , , cord-351964-hduv0ur4 206 3 0.1 0.1 CD cord-351964-hduv0ur4 206 4 lun lun NNS cord-351964-hduv0ur4 206 5 . . . cord-351964-hduv0ur4 207 1 Figures figure NNS cord-351964-hduv0ur4 207 2 8 8 CD cord-351964-hduv0ur4 207 3 - - SYM cord-351964-hduv0ur4 207 4 10 10 CD cord-351964-hduv0ur4 207 5 . . . cord-351964-hduv0ur4 208 1 ( ( -LRB- cord-351964-hduv0ur4 208 2 Fig Fig NNP cord-351964-hduv0ur4 208 3 . . . cord-351964-hduv0ur4 208 4 8) 8) NNP cord-351964-hduv0ur4 209 1 Condensing condense VBG cord-351964-hduv0ur4 209 2 secretory secretory NN cord-351964-hduv0ur4 209 3 proteins protein NNS cord-351964-hduv0ur4 209 4 in in IN cord-351964-hduv0ur4 209 5 the the DT cord-351964-hduv0ur4 209 6 trans trans NNP cord-351964-hduv0ur4 209 7 - - NNP cord-351964-hduv0ur4 209 8 Golgi Golgi NNP cord-351964-hduv0ur4 209 9 network network NN cord-351964-hduv0ur4 209 10 of of IN cord-351964-hduv0ur4 209 11 an an DT cord-351964-hduv0ur4 209 12 uninfected uninfected JJ cord-351964-hduv0ur4 209 13 AtT20 AtT20 NNP cord-351964-hduv0ur4 209 14 cell cell NN cord-351964-hduv0ur4 209 15 . . . cord-351964-hduv0ur4 210 1 The the DT cord-351964-hduv0ur4 210 2 condensing condense VBG cord-351964-hduv0ur4 210 3 secretory secretory JJ cord-351964-hduv0ur4 210 4 protein protein NN cord-351964-hduv0ur4 210 5 ( ( -LRB- cord-351964-hduv0ur4 210 6 open open JJ cord-351964-hduv0ur4 210 7 arrows arrow NNS cord-351964-hduv0ur4 210 8 ) ) -RRB- cord-351964-hduv0ur4 210 9 is be VBZ cord-351964-hduv0ur4 210 10 within within IN cord-351964-hduv0ur4 210 11 two two CD cord-351964-hduv0ur4 210 12 dilations dilation NNS cord-351964-hduv0ur4 210 13 of of IN cord-351964-hduv0ur4 210 14 the the DT cord-351964-hduv0ur4 210 15 trans trans NNP cord-351964-hduv0ur4 210 16 - - NNP cord-351964-hduv0ur4 210 17 Golgi Golgi NNP cord-351964-hduv0ur4 210 18 network network NN cord-351964-hduv0ur4 210 19 . . . cord-351964-hduv0ur4 211 1 Note note VB cord-351964-hduv0ur4 211 2 the the DT cord-351964-hduv0ur4 211 3 clathrin clathrin NN cord-351964-hduv0ur4 211 4 coat coat NN cord-351964-hduv0ur4 211 5 on on IN cord-351964-hduv0ur4 211 6 parts part NNS cord-351964-hduv0ur4 211 7 of of IN cord-351964-hduv0ur4 211 8 the the DT cord-351964-hduv0ur4 211 9 membrane membrane NN cord-351964-hduv0ur4 211 10 ( ( -LRB- cord-351964-hduv0ur4 211 11 between between IN cord-351964-hduv0ur4 211 12 the the DT cord-351964-hduv0ur4 211 13 arrows arrow NNS cord-351964-hduv0ur4 211 14 ) ) -RRB- cord-351964-hduv0ur4 211 15 and and CC cord-351964-hduv0ur4 211 16 also also RB cord-351964-hduv0ur4 211 17 coated coat VBD cord-351964-hduv0ur4 211 18 vesicles vesicle NNS cord-351964-hduv0ur4 211 19 . . . cord-351964-hduv0ur4 212 1 Interestingly interestingly RB cord-351964-hduv0ur4 212 2 , , , cord-351964-hduv0ur4 212 3 on on IN cord-351964-hduv0ur4 212 4 the the DT cord-351964-hduv0ur4 212 5 cis cis NNP cord-351964-hduv0ur4 212 6 - - HYPH cord-351964-hduv0ur4 212 7 side side NN cord-351964-hduv0ur4 212 8 of of IN cord-351964-hduv0ur4 212 9 the the DT cord-351964-hduv0ur4 212 10 Golgi Golgi NNP cord-351964-hduv0ur4 212 11 stack stack VB cord-351964-hduv0ur4 212 12 a a DT cord-351964-hduv0ur4 212 13 sheet sheet NN cord-351964-hduv0ur4 212 14 of of IN cord-351964-hduv0ur4 212 15 rough rough JJ cord-351964-hduv0ur4 212 16 endoplasmic endoplasmic NN cord-351964-hduv0ur4 212 17 reticulum reticulum NN cord-351964-hduv0ur4 212 18 is be VBZ cord-351964-hduv0ur4 212 19 differentiated differentiate VBN cord-351964-hduv0ur4 212 20 in in IN cord-351964-hduv0ur4 212 21 one one CD cord-351964-hduv0ur4 212 22 region region NN cord-351964-hduv0ur4 212 23 into into IN cord-351964-hduv0ur4 212 24 annulate annulate NN cord-351964-hduv0ur4 212 25 lamellae lamella NNS cord-351964-hduv0ur4 212 26 ( ( -LRB- cord-351964-hduv0ur4 212 27 AL AL NNP cord-351964-hduv0ur4 212 28 ) ) -RRB- cord-351964-hduv0ur4 212 29 and and CC cord-351964-hduv0ur4 212 30 in in IN cord-351964-hduv0ur4 212 31 another another DT cord-351964-hduv0ur4 212 32 into into IN cord-351964-hduv0ur4 212 33 a a DT cord-351964-hduv0ur4 212 34 transitional transitional JJ cord-351964-hduv0ur4 212 35 element element NN cord-351964-hduv0ur4 212 36 ( ( -LRB- cord-351964-hduv0ur4 212 37 TE TE NNP cord-351964-hduv0ur4 212 38 ) ) -RRB- cord-351964-hduv0ur4 212 39 . . . cord-351964-hduv0ur4 213 1 Apparently apparently RB cord-351964-hduv0ur4 213 2 two two CD cord-351964-hduv0ur4 213 3 morphological morphological JJ cord-351964-hduv0ur4 213 4 differentiations differentiation NNS cord-351964-hduv0ur4 213 5 of of IN cord-351964-hduv0ur4 213 6 the the DT cord-351964-hduv0ur4 213 7 rough rough JJ cord-351964-hduv0ur4 213 8 endoplasmic endoplasmic NN cord-351964-hduv0ur4 213 9 reticulum reticulum NN cord-351964-hduv0ur4 213 10 can can MD cord-351964-hduv0ur4 213 11 occur occur VB cord-351964-hduv0ur4 213 12 in in IN cord-351964-hduv0ur4 213 13 very very RB cord-351964-hduv0ur4 213 14 close close JJ cord-351964-hduv0ur4 213 15 proximity proximity NN cord-351964-hduv0ur4 213 16 in in IN cord-351964-hduv0ur4 213 17 these these DT cord-351964-hduv0ur4 213 18 tumor tumor NN cord-351964-hduv0ur4 213 19 cells cell NNS cord-351964-hduv0ur4 213 20 . . . cord-351964-hduv0ur4 214 1 Bar bar NN cord-351964-hduv0ur4 214 2 , , , cord-351964-hduv0ur4 214 3 0.1 0.1 CD cord-351964-hduv0ur4 214 4 Inn Inn NNP cord-351964-hduv0ur4 214 5 . . . cord-351964-hduv0ur4 215 1 ( ( -LRB- cord-351964-hduv0ur4 215 2 Fig Fig NNP cord-351964-hduv0ur4 215 3 . . . cord-351964-hduv0ur4 215 4 9 9 CD cord-351964-hduv0ur4 215 5 ) ) -RRB- cord-351964-hduv0ur4 215 6 Condensed condense VBN cord-351964-hduv0ur4 215 7 virions virion NNS cord-351964-hduv0ur4 215 8 in in IN cord-351964-hduv0ur4 215 9 the the DT cord-351964-hduv0ur4 215 10 trans trans NNP cord-351964-hduv0ur4 215 11 - - NNP cord-351964-hduv0ur4 215 12 Golgi Golgi NNP cord-351964-hduv0ur4 215 13 network network NN cord-351964-hduv0ur4 215 14 . . . cord-351964-hduv0ur4 216 1 Note note VB cord-351964-hduv0ur4 216 2 the the DT cord-351964-hduv0ur4 216 3 prominent prominent JJ cord-351964-hduv0ur4 216 4 coat coat NN cord-351964-hduv0ur4 216 5 on on IN cord-351964-hduv0ur4 216 6 the the DT cord-351964-hduv0ur4 216 7 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 216 8 face face NN cord-351964-hduv0ur4 216 9 of of IN cord-351964-hduv0ur4 216 10 one one CD cord-351964-hduv0ur4 216 11 of of IN cord-351964-hduv0ur4 216 12 the the DT cord-351964-hduv0ur4 216 13 compartments compartment NNS cord-351964-hduv0ur4 216 14 enclosing enclose VBG cord-351964-hduv0ur4 216 15 a a DT cord-351964-hduv0ur4 216 16 mature mature JJ cord-351964-hduv0ur4 216 17 virion virion NN cord-351964-hduv0ur4 216 18 ( ( -LRB- cord-351964-hduv0ur4 216 19 arrow arrow NN cord-351964-hduv0ur4 216 20 ) ) -RRB- cord-351964-hduv0ur4 216 21 . . . cord-351964-hduv0ur4 217 1 The the DT cord-351964-hduv0ur4 217 2 coat coat NN cord-351964-hduv0ur4 217 3 has have VBZ cord-351964-hduv0ur4 217 4 the the DT cord-351964-hduv0ur4 217 5 characteristic characteristic JJ cord-351964-hduv0ur4 217 6 morphology morphology NN cord-351964-hduv0ur4 217 7 of of IN cord-351964-hduv0ur4 217 8 clathrin clathrin NN cord-351964-hduv0ur4 217 9 . . . cord-351964-hduv0ur4 218 1 Bar bar NN cord-351964-hduv0ur4 218 2 , , , cord-351964-hduv0ur4 218 3 0.1 0.1 CD cord-351964-hduv0ur4 218 4 I -PRON- PRP cord-351964-hduv0ur4 218 5 m be NN cord-351964-hduv0ur4 218 6 a going to NN cord-351964-hduv0ur4 218 7 . . . cord-351964-hduv0ur4 219 1 ( ( -LRB- cord-351964-hduv0ur4 219 2 Fig Fig NNP cord-351964-hduv0ur4 219 3 . . . cord-351964-hduv0ur4 220 1 10 10 CD cord-351964-hduv0ur4 220 2 ) ) -RRB- cord-351964-hduv0ur4 221 1 Part part NN cord-351964-hduv0ur4 221 2 of of IN cord-351964-hduv0ur4 221 3 an an DT cord-351964-hduv0ur4 221 4 infected infected JJ cord-351964-hduv0ur4 221 5 cell cell NN cord-351964-hduv0ur4 221 6 exposed expose VBN cord-351964-hduv0ur4 221 7 to to IN cord-351964-hduv0ur4 221 8 200 200 CD cord-351964-hduv0ur4 221 9 I i NN cord-351964-hduv0ur4 221 10 ~ ~ NFP cord-351964-hduv0ur4 221 11 M m NN cord-351964-hduv0ur4 221 12 chloroquine chloroquine NN cord-351964-hduv0ur4 221 13 from from IN cord-351964-hduv0ur4 221 14 7.5-to 7.5-to CD cord-351964-hduv0ur4 221 15 9-h 9-h CD cord-351964-hduv0ur4 221 16 postinfection postinfection NN cord-351964-hduv0ur4 221 17 . . . cord-351964-hduv0ur4 222 1 Note note VB cord-351964-hduv0ur4 222 2 the the DT cord-351964-hduv0ur4 222 3 large large JJ cord-351964-hduv0ur4 222 4 accumulations accumulation NNS cord-351964-hduv0ur4 222 5 of of IN cord-351964-hduv0ur4 222 6 condensed condense VBN cord-351964-hduv0ur4 222 7 virions virion NNS cord-351964-hduv0ur4 222 8 in in IN cord-351964-hduv0ur4 222 9 the the DT cord-351964-hduv0ur4 222 10 trans trans NNP cord-351964-hduv0ur4 222 11 - - NNP cord-351964-hduv0ur4 222 12 Golgi Golgi NNP cord-351964-hduv0ur4 222 13 network network NN cord-351964-hduv0ur4 222 14 ( ( -LRB- cord-351964-hduv0ur4 222 15 arrows arrow NNS cord-351964-hduv0ur4 222 16 ) ) -RRB- cord-351964-hduv0ur4 222 17 and and CC cord-351964-hduv0ur4 222 18 immature immature JJ cord-351964-hduv0ur4 222 19 uncondensed uncondensed JJ cord-351964-hduv0ur4 222 20 virions virion NNS cord-351964-hduv0ur4 222 21 in in IN cord-351964-hduv0ur4 222 22 the the DT cord-351964-hduv0ur4 222 23 Golgi Golgi NNP cord-351964-hduv0ur4 222 24 cisternae cisternae NN cord-351964-hduv0ur4 222 25 ( ( -LRB- cord-351964-hduv0ur4 222 26 arrowheads arrowhead NNS cord-351964-hduv0ur4 222 27 ) ) -RRB- cord-351964-hduv0ur4 222 28 . . . cord-351964-hduv0ur4 223 1 Post Post NNP cord-351964-hduv0ur4 223 2 - - JJ cord-351964-hduv0ur4 223 3 Golgi Golgi NNP cord-351964-hduv0ur4 223 4 transport transport NN cord-351964-hduv0ur4 223 5 vesicles vesicle NNS cord-351964-hduv0ur4 223 6 packed pack VBN cord-351964-hduv0ur4 223 7 with with IN cord-351964-hduv0ur4 223 8 mature mature JJ cord-351964-hduv0ur4 223 9 virions virion NNS cord-351964-hduv0ur4 223 10 can can MD cord-351964-hduv0ur4 223 11 be be VB cord-351964-hduv0ur4 223 12 seen see VBN cord-351964-hduv0ur4 223 13 below below IN cord-351964-hduv0ur4 223 14 the the DT cord-351964-hduv0ur4 223 15 plasma plasma NN cord-351964-hduv0ur4 223 16 membrane membrane NN cord-351964-hduv0ur4 223 17 ( ( -LRB- cord-351964-hduv0ur4 223 18 open open JJ cord-351964-hduv0ur4 223 19 arrows arrow NNS cord-351964-hduv0ur4 223 20 ) ) -RRB- cord-351964-hduv0ur4 223 21 . . . cord-351964-hduv0ur4 224 1 Bar bar NN cord-351964-hduv0ur4 224 2 , , , cord-351964-hduv0ur4 224 3 0.5 0.5 CD cord-351964-hduv0ur4 224 4 ~tm ~tm NNP cord-351964-hduv0ur4 224 5 . . . cord-351964-hduv0ur4 225 1 Figure figure NN cord-351964-hduv0ur4 225 2 11 11 CD cord-351964-hduv0ur4 225 3 . . . cord-351964-hduv0ur4 226 1 Post Post NNP cord-351964-hduv0ur4 226 2 - - JJ cord-351964-hduv0ur4 226 3 Golgi Golgi NNP cord-351964-hduv0ur4 226 4 transport transport NN cord-351964-hduv0ur4 226 5 vesicles vesicle NNS cord-351964-hduv0ur4 226 6 and and CC cord-351964-hduv0ur4 226 7 secretory secretory NN cord-351964-hduv0ur4 226 8 granules granule NNS cord-351964-hduv0ur4 226 9 close close RB cord-351964-hduv0ur4 226 10 to to IN cord-351964-hduv0ur4 226 11 the the DT cord-351964-hduv0ur4 226 12 lower low JJR cord-351964-hduv0ur4 226 13 surface surface NN cord-351964-hduv0ur4 226 14 of of IN cord-351964-hduv0ur4 226 15 infected infected JJ cord-351964-hduv0ur4 226 16 AtT20 AtT20 NNP cord-351964-hduv0ur4 226 17 cells cell NNS cord-351964-hduv0ur4 226 18 , , , cord-351964-hduv0ur4 226 19 growing grow VBG cord-351964-hduv0ur4 226 20 on on IN cord-351964-hduv0ur4 226 21 extracellular extracellular JJ cord-351964-hduv0ur4 226 22 matrix matrix NN cord-351964-hduv0ur4 226 23 , , , cord-351964-hduv0ur4 226 24 after after IN cord-351964-hduv0ur4 226 25 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 226 26 labeling labeling NN cord-351964-hduv0ur4 226 27 with with IN cord-351964-hduv0ur4 226 28 anti anti JJ cord-351964-hduv0ur4 226 29 - - JJ cord-351964-hduv0ur4 226 30 ACTH acth JJ cord-351964-hduv0ur4 226 31 antibody antibody NN cord-351964-hduv0ur4 226 32 . . . cord-351964-hduv0ur4 227 1 In in IN cord-351964-hduv0ur4 227 2 A a DT cord-351964-hduv0ur4 227 3 note note NN cord-351964-hduv0ur4 227 4 that that IN cord-351964-hduv0ur4 227 5 the the DT cord-351964-hduv0ur4 227 6 secretory secretory JJ cord-351964-hduv0ur4 227 7 granules granule NNS cord-351964-hduv0ur4 227 8 are be VBP cord-351964-hduv0ur4 227 9 heavily heavily RB cord-351964-hduv0ur4 227 10 labeled label VBN cord-351964-hduv0ur4 227 11 for for IN cord-351964-hduv0ur4 227 12 ACTH ACTH NNP cord-351964-hduv0ur4 227 13 ( ( -LRB- cord-351964-hduv0ur4 227 14 arrows arrow NNS cord-351964-hduv0ur4 227 15 ) ) -RRB- cord-351964-hduv0ur4 227 16 . . . cord-351964-hduv0ur4 228 1 A a DT cord-351964-hduv0ur4 228 2 completely completely RB cord-351964-hduv0ur4 228 3 unlabeled unlabeled JJ cord-351964-hduv0ur4 228 4 transport transport NN cord-351964-hduv0ur4 228 5 vesicle vesicle NN cord-351964-hduv0ur4 228 6 packed pack VBN cord-351964-hduv0ur4 228 7 with with IN cord-351964-hduv0ur4 228 8 virions virion NNS cord-351964-hduv0ur4 228 9 ( ( -LRB- cord-351964-hduv0ur4 228 10 arrowhead arrowhead NNP cord-351964-hduv0ur4 228 11 ) ) -RRB- cord-351964-hduv0ur4 228 12 is be VBZ cord-351964-hduv0ur4 228 13 close close JJ cord-351964-hduv0ur4 228 14 to to IN cord-351964-hduv0ur4 228 15 the the DT cord-351964-hduv0ur4 228 16 lower low JJR cord-351964-hduv0ur4 228 17 cell cell NN cord-351964-hduv0ur4 228 18 surface surface NN cord-351964-hduv0ur4 228 19 , , , cord-351964-hduv0ur4 228 20 which which WDT cord-351964-hduv0ur4 228 21 lies lie VBZ cord-351964-hduv0ur4 228 22 on on IN cord-351964-hduv0ur4 228 23 the the DT cord-351964-hduv0ur4 228 24 layer layer NN cord-351964-hduv0ur4 228 25 of of IN cord-351964-hduv0ur4 228 26 extra extra JJ cord-351964-hduv0ur4 228 27 ~ ~ NNP cord-351964-hduv0ur4 228 28 llular llular NNP cord-351964-hduv0ur4 228 29 matrix matrix NN cord-351964-hduv0ur4 228 30 ( ( -LRB- cord-351964-hduv0ur4 228 31 ECM ECM NNP cord-351964-hduv0ur4 228 32 ) ) -RRB- cord-351964-hduv0ur4 228 33 . . . cord-351964-hduv0ur4 229 1 B b NN cord-351964-hduv0ur4 229 2 shows show NNS cord-351964-hduv0ur4 229 3 , , , cord-351964-hduv0ur4 229 4 near near IN cord-351964-hduv0ur4 229 5 the the DT cord-351964-hduv0ur4 229 6 lower low JJR cord-351964-hduv0ur4 229 7 surface surface NN cord-351964-hduv0ur4 229 8 of of IN cord-351964-hduv0ur4 229 9 a a DT cord-351964-hduv0ur4 229 10 cell cell NN cord-351964-hduv0ur4 229 11 growing grow VBG cord-351964-hduv0ur4 229 12 on on IN cord-351964-hduv0ur4 229 13 ECM ECM NNP cord-351964-hduv0ur4 229 14 , , , cord-351964-hduv0ur4 229 15 one one CD cord-351964-hduv0ur4 229 16 of of IN cord-351964-hduv0ur4 229 17 the the DT cord-351964-hduv0ur4 229 18 extremely extremely RB cord-351964-hduv0ur4 229 19 rare rare JJ cord-351964-hduv0ur4 229 20 classes class NNS cord-351964-hduv0ur4 229 21 of of IN cord-351964-hduv0ur4 229 22 post post JJ cord-351964-hduv0ur4 229 23 - - JJ cord-351964-hduv0ur4 229 24 Golgi golgi JJ cord-351964-hduv0ur4 229 25 transport transport NN cord-351964-hduv0ur4 229 26 vesicles vesicle NNS cord-351964-hduv0ur4 229 27 which which WDT cord-351964-hduv0ur4 229 28 contain contain VBP cord-351964-hduv0ur4 229 29 both both DT cord-351964-hduv0ur4 229 30 virions virion NNS cord-351964-hduv0ur4 229 31 ( ( -LRB- cord-351964-hduv0ur4 229 32 arrows arrow NNS cord-351964-hduv0ur4 229 33 ) ) -RRB- cord-351964-hduv0ur4 229 34 and and CC cord-351964-hduv0ur4 229 35 a a DT cord-351964-hduv0ur4 229 36 clump clump NN cord-351964-hduv0ur4 229 37 of of IN cord-351964-hduv0ur4 229 38 condensed condense VBN cord-351964-hduv0ur4 229 39 secretory secretory JJ cord-351964-hduv0ur4 229 40 protein protein NN cord-351964-hduv0ur4 229 41 that that WDT cord-351964-hduv0ur4 229 42 labels label VBZ cord-351964-hduv0ur4 229 43 heavily heavily RB cord-351964-hduv0ur4 229 44 with with IN cord-351964-hduv0ur4 229 45 anti anti JJ cord-351964-hduv0ur4 229 46 - - JJ cord-351964-hduv0ur4 229 47 ACTH acth JJ cord-351964-hduv0ur4 229 48 antibody antibody NN cord-351964-hduv0ur4 229 49 ( ( -LRB- cord-351964-hduv0ur4 229 50 arrowhead arrowhead NNP cord-351964-hduv0ur4 229 51 ) ) -RRB- cord-351964-hduv0ur4 229 52 . . . cord-351964-hduv0ur4 230 1 Some some DT cord-351964-hduv0ur4 230 2 of of IN cord-351964-hduv0ur4 230 3 the the DT cord-351964-hduv0ur4 230 4 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 230 5 reaction reaction NN cord-351964-hduv0ur4 230 6 product product NN cord-351964-hduv0ur4 230 7 has have VBZ cord-351964-hduv0ur4 230 8 diffused diffuse VBN cord-351964-hduv0ur4 230 9 to to TO cord-351964-hduv0ur4 230 10 coat coat VB cord-351964-hduv0ur4 230 11 the the DT cord-351964-hduv0ur4 230 12 virions virion NNS cord-351964-hduv0ur4 230 13 and and CC cord-351964-hduv0ur4 230 14 the the DT cord-351964-hduv0ur4 230 15 membrane membrane NN cord-351964-hduv0ur4 230 16 of of IN cord-351964-hduv0ur4 230 17 the the DT cord-351964-hduv0ur4 230 18 vesicle vesicle NN cord-351964-hduv0ur4 230 19 . . . cord-351964-hduv0ur4 231 1 Bars bar NNS cord-351964-hduv0ur4 231 2 , , , cord-351964-hduv0ur4 231 3 0.1 0.1 CD cord-351964-hduv0ur4 231 4 lain lain NN cord-351964-hduv0ur4 231 5 . . . cord-351964-hduv0ur4 231 6 1986 1986 CD cord-351964-hduv0ur4 231 7 ) ) -RRB- cord-351964-hduv0ur4 231 8 . . . cord-351964-hduv0ur4 232 1 ( ( -LRB- cord-351964-hduv0ur4 232 2 b b LS cord-351964-hduv0ur4 232 3 ) ) -RRB- cord-351964-hduv0ur4 232 4 That that IN cord-351964-hduv0ur4 232 5 within within IN cord-351964-hduv0ur4 232 6 the the DT cord-351964-hduv0ur4 232 7 trans trans NNP cord-351964-hduv0ur4 232 8 - - NNP cord-351964-hduv0ur4 232 9 Golgi Golgi NNP cord-351964-hduv0ur4 232 10 network network NN cord-351964-hduv0ur4 232 11 secretory secretory NN cord-351964-hduv0ur4 232 12 proteins protein NNS cord-351964-hduv0ur4 232 13 condense condense VBP cord-351964-hduv0ur4 232 14 into into IN cord-351964-hduv0ur4 232 15 granule granule NN cord-351964-hduv0ur4 232 16 cores core NNS cord-351964-hduv0ur4 232 17 that that WDT cord-351964-hduv0ur4 232 18 exclude exclude VBP cord-351964-hduv0ur4 232 19 virions virion NNS cord-351964-hduv0ur4 232 20 . . . cord-351964-hduv0ur4 233 1 ( ( -LRB- cord-351964-hduv0ur4 233 2 c c NN cord-351964-hduv0ur4 233 3 ) ) -RRB- cord-351964-hduv0ur4 233 4 Beyond beyond IN cord-351964-hduv0ur4 233 5 the the DT cord-351964-hduv0ur4 233 6 trans trans JJ cord-351964-hduv0ur4 233 7 - - NNP cord-351964-hduv0ur4 233 8 Golgi Golgi NNP cord-351964-hduv0ur4 233 9 network network NN cord-351964-hduv0ur4 233 10 virions virion NNS cord-351964-hduv0ur4 233 11 are be VBP cord-351964-hduv0ur4 233 12 packed pack VBN cord-351964-hduv0ur4 233 13 in in IN cord-351964-hduv0ur4 233 14 vesicles vesicle NNS cord-351964-hduv0ur4 233 15 lacking lack VBG cord-351964-hduv0ur4 233 16 condensed condense VBN cord-351964-hduv0ur4 233 17 secretory secretory JJ cord-351964-hduv0ur4 233 18 proteins protein NNS cord-351964-hduv0ur4 233 19 . . . cord-351964-hduv0ur4 234 1 We -PRON- PRP cord-351964-hduv0ur4 234 2 attempted attempt VBD cord-351964-hduv0ur4 234 3 to to TO cord-351964-hduv0ur4 234 4 confirm confirm VB cord-351964-hduv0ur4 234 5 this this DT cord-351964-hduv0ur4 234 6 sorting sort VBG cord-351964-hduv0ur4 234 7 ofvirions ofvirion NNS cord-351964-hduv0ur4 234 8 from from IN cord-351964-hduv0ur4 234 9 secretory secretory JJ cord-351964-hduv0ur4 234 10 proteins protein NNS cord-351964-hduv0ur4 234 11 entering enter VBG cord-351964-hduv0ur4 234 12 granules granule NNS cord-351964-hduv0ur4 234 13 of of IN cord-351964-hduv0ur4 234 14 the the DT cord-351964-hduv0ur4 234 15 regulated regulated JJ cord-351964-hduv0ur4 234 16 exocytic exocytic JJ cord-351964-hduv0ur4 234 17 pathway pathway NN cord-351964-hduv0ur4 234 18 . . . cord-351964-hduv0ur4 235 1 Infected infected JJ cord-351964-hduv0ur4 235 2 cultures culture NNS cord-351964-hduv0ur4 235 3 were be VBD cord-351964-hduv0ur4 235 4 labeled label VBN cord-351964-hduv0ur4 235 5 using use VBG cord-351964-hduv0ur4 235 6 an an DT cord-351964-hduv0ur4 235 7 affinity affinity NN cord-351964-hduv0ur4 235 8 - - HYPH cord-351964-hduv0ur4 235 9 purified purify VBN cord-351964-hduv0ur4 235 10 antiserum antiserum NN cord-351964-hduv0ur4 235 11 against against IN cord-351964-hduv0ur4 235 12 ACrH acrh NN cord-351964-hduv0ur4 235 13 by by IN cord-351964-hduv0ur4 235 14 the the DT cord-351964-hduv0ur4 235 15 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 235 16 method method NN cord-351964-hduv0ur4 235 17 , , , cord-351964-hduv0ur4 235 18 and and CC cord-351964-hduv0ur4 235 19 the the DT cord-351964-hduv0ur4 235 20 post post JJ cord-351964-hduv0ur4 235 21 - - JJ cord-351964-hduv0ur4 235 22 Golgi golgi JJ cord-351964-hduv0ur4 235 23 exocytic exocytic JJ cord-351964-hduv0ur4 235 24 compartments compartment NNS cord-351964-hduv0ur4 235 25 were be VBD cord-351964-hduv0ur4 235 26 examined examine VBN cord-351964-hduv0ur4 235 27 . . . cord-351964-hduv0ur4 236 1 This this DT cord-351964-hduv0ur4 236 2 labeling labeling NN cord-351964-hduv0ur4 236 3 reveals reveal VBZ cord-351964-hduv0ur4 236 4 ACTH ACTH NNP cord-351964-hduv0ur4 236 5 in in IN cord-351964-hduv0ur4 236 6 all all DT cord-351964-hduv0ur4 236 7 compartments compartment NNS cord-351964-hduv0ur4 236 8 of of IN cord-351964-hduv0ur4 236 9 the the DT cord-351964-hduv0ur4 236 10 exocytic exocytic JJ cord-351964-hduv0ur4 236 11 pathway pathway NN cord-351964-hduv0ur4 236 12 from from IN cord-351964-hduv0ur4 236 13 the the DT cord-351964-hduv0ur4 236 14 rough rough JJ cord-351964-hduv0ur4 236 15 endoplasmic endoplasmic NN cord-351964-hduv0ur4 236 16 reticulum reticulum NN cord-351964-hduv0ur4 236 17 onwards onwards RB cord-351964-hduv0ur4 236 18 . . . cord-351964-hduv0ur4 237 1 The the DT cord-351964-hduv0ur4 237 2 post post JJ cord-351964-hduv0ur4 237 3 - - JJ cord-351964-hduv0ur4 237 4 Golgi golgi JJ cord-351964-hduv0ur4 237 5 compartments compartment NNS cord-351964-hduv0ur4 237 6 that that WDT cord-351964-hduv0ur4 237 7 occurred occur VBD cord-351964-hduv0ur4 237 8 below below IN cord-351964-hduv0ur4 237 9 the the DT cord-351964-hduv0ur4 237 10 plasma plasma NN cord-351964-hduv0ur4 237 11 membrane membrane NN cord-351964-hduv0ur4 237 12 at at IN cord-351964-hduv0ur4 237 13 the the DT cord-351964-hduv0ur4 237 14 cell cell NN cord-351964-hduv0ur4 237 15 periphery periphery NN cord-351964-hduv0ur4 237 16 and and CC cord-351964-hduv0ur4 237 17 lacked lack VBD cord-351964-hduv0ur4 237 18 coats coat NNS cord-351964-hduv0ur4 237 19 on on IN cord-351964-hduv0ur4 237 20 their -PRON- PRP$ cord-351964-hduv0ur4 237 21 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 237 22 surface surface NN cord-351964-hduv0ur4 237 23 fell fall VBD cord-351964-hduv0ur4 237 24 into into IN cord-351964-hduv0ur4 237 25 three three CD cord-351964-hduv0ur4 237 26 categories category NNS cord-351964-hduv0ur4 237 27 : : : cord-351964-hduv0ur4 237 28 ( ( -LRB- cord-351964-hduv0ur4 237 29 a a LS cord-351964-hduv0ur4 237 30 ) ) -RRB- cord-351964-hduv0ur4 237 31 labeled label VBN cord-351964-hduv0ur4 237 32 secretory secretory JJ cord-351964-hduv0ur4 237 33 granules granule NNS cord-351964-hduv0ur4 237 34 that that WDT cord-351964-hduv0ur4 237 35 did do VBD cord-351964-hduv0ur4 237 36 not not RB cord-351964-hduv0ur4 237 37 contain contain VB cord-351964-hduv0ur4 237 38 coronavirions coronavirion NNS cord-351964-hduv0ur4 237 39 and and CC cord-351964-hduv0ur4 237 40 were be VBD cord-351964-hduv0ur4 237 41 identical identical JJ cord-351964-hduv0ur4 237 42 to to IN cord-351964-hduv0ur4 237 43 secretory secretory JJ cord-351964-hduv0ur4 237 44 granules granule NNS cord-351964-hduv0ur4 237 45 in in IN cord-351964-hduv0ur4 237 46 uninfected uninfected JJ cord-351964-hduv0ur4 237 47 cells cell NNS cord-351964-hduv0ur4 237 48 ( ( -LRB- cord-351964-hduv0ur4 237 49 Fig fig NN cord-351964-hduv0ur4 237 50 . . . cord-351964-hduv0ur4 237 51 11 11 CD cord-351964-hduv0ur4 237 52 A a NN cord-351964-hduv0ur4 237 53 ) ) -RRB- cord-351964-hduv0ur4 237 54 ; ; : cord-351964-hduv0ur4 238 1 ( ( -LRB- cord-351964-hduv0ur4 238 2 b b LS cord-351964-hduv0ur4 238 3 ) ) -RRB- cord-351964-hduv0ur4 238 4 post post VB cord-351964-hduv0ur4 238 5 - - JJ cord-351964-hduv0ur4 238 6 Golgi golgi JJ cord-351964-hduv0ur4 238 7 vesicles vesicle NNS cord-351964-hduv0ur4 238 8 packed pack VBN cord-351964-hduv0ur4 238 9 with with IN cord-351964-hduv0ur4 238 10 many many JJ cord-351964-hduv0ur4 238 11 virions virion NNS cord-351964-hduv0ur4 238 12 but but CC cord-351964-hduv0ur4 238 13 either either CC cord-351964-hduv0ur4 238 14 not not RB cord-351964-hduv0ur4 238 15 labeled label VBN cord-351964-hduv0ur4 238 16 for for IN cord-351964-hduv0ur4 238 17 ACTH ACTH NNP cord-351964-hduv0ur4 238 18 ( ( -LRB- cord-351964-hduv0ur4 238 19 Fig Fig NNP cord-351964-hduv0ur4 238 20 . . . cord-351964-hduv0ur4 238 21 11 11 CD cord-351964-hduv0ur4 238 22 A A NNP cord-351964-hduv0ur4 238 23 ) ) -RRB- cord-351964-hduv0ur4 238 24 or or CC cord-351964-hduv0ur4 238 25 with with IN cord-351964-hduv0ur4 238 26 traces trace NNS cord-351964-hduv0ur4 238 27 of of IN cord-351964-hduv0ur4 238 28 reaction reaction NN cord-351964-hduv0ur4 238 29 product product NN cord-351964-hduv0ur4 238 30 on on IN cord-351964-hduv0ur4 238 31 the the DT cord-351964-hduv0ur4 238 32 vesicles vesicle NNS cord-351964-hduv0ur4 238 33 inner inner JJ cord-351964-hduv0ur4 238 34 surface surface NN cord-351964-hduv0ur4 238 35 ( ( -LRB- cord-351964-hduv0ur4 238 36 in in IN cord-351964-hduv0ur4 238 37 this this DT cord-351964-hduv0ur4 238 38 class class NN cord-351964-hduv0ur4 238 39 of of IN cord-351964-hduv0ur4 238 40 vesicles vesicle NNS cord-351964-hduv0ur4 238 41 there there EX cord-351964-hduv0ur4 238 42 was be VBD cord-351964-hduv0ur4 238 43 no no DT cord-351964-hduv0ur4 238 44 evidence evidence NN cord-351964-hduv0ur4 238 45 of of IN cord-351964-hduv0ur4 238 46 clumps clump NNS cord-351964-hduv0ur4 238 47 of of IN cord-351964-hduv0ur4 238 48 condensed condense VBN cord-351964-hduv0ur4 238 49 secretory secretory JJ cord-351964-hduv0ur4 238 50 proteins protein NNS cord-351964-hduv0ur4 238 51 ) ) -RRB- cord-351964-hduv0ur4 238 52 ; ; : cord-351964-hduv0ur4 238 53 ( ( -LRB- cord-351964-hduv0ur4 238 54 c c NN cord-351964-hduv0ur4 238 55 ) ) -RRB- cord-351964-hduv0ur4 238 56 extremely extremely RB cord-351964-hduv0ur4 238 57 rare rare JJ cord-351964-hduv0ur4 238 58 vesicles vesicle NNS cord-351964-hduv0ur4 238 59 containing contain VBG cord-351964-hduv0ur4 238 60 both both DT cord-351964-hduv0ur4 238 61 virions virion NNS cord-351964-hduv0ur4 238 62 and and CC cord-351964-hduv0ur4 238 63 clumps clump NNS cord-351964-hduv0ur4 238 64 of of IN cord-351964-hduv0ur4 238 65 condensed condense VBN cord-351964-hduv0ur4 238 66 secretory secretory JJ cord-351964-hduv0ur4 238 67 proteins protein NNS cord-351964-hduv0ur4 238 68 ( ( -LRB- cord-351964-hduv0ur4 238 69 Fig fig NN cord-351964-hduv0ur4 238 70 . . . cord-351964-hduv0ur4 239 1 U U NNP cord-351964-hduv0ur4 239 2 B B NNP cord-351964-hduv0ur4 239 3 ) ) -RRB- cord-351964-hduv0ur4 239 4 that that WDT cord-351964-hduv0ur4 239 5 resemble resemble VBP cord-351964-hduv0ur4 239 6 in in IN cord-351964-hduv0ur4 239 7 their -PRON- PRP$ cord-351964-hduv0ur4 239 8 labeling labeling NN cord-351964-hduv0ur4 239 9 for for IN cord-351964-hduv0ur4 239 10 ACTH ACTH NNP cord-351964-hduv0ur4 239 11 the the DT cord-351964-hduv0ur4 239 12 core core NN cord-351964-hduv0ur4 239 13 material material NN cord-351964-hduv0ur4 239 14 of of IN cord-351964-hduv0ur4 239 15 secretory secretory JJ cord-351964-hduv0ur4 239 16 granules granule NNS cord-351964-hduv0ur4 239 17 in in IN cord-351964-hduv0ur4 239 18 both both CC cord-351964-hduv0ur4 239 19 infected infected JJ cord-351964-hduv0ur4 239 20 and and CC cord-351964-hduv0ur4 239 21 uninfected uninfected JJ cord-351964-hduv0ur4 239 22 cells cell NNS cord-351964-hduv0ur4 239 23 ( ( -LRB- cord-351964-hduv0ur4 239 24 compare compare VBP cord-351964-hduv0ur4 239 25 Fig Fig NNP cord-351964-hduv0ur4 239 26 . . . cord-351964-hduv0ur4 240 1 11 11 CD cord-351964-hduv0ur4 240 2 A a NN cord-351964-hduv0ur4 240 3 and and CC cord-351964-hduv0ur4 240 4 11 11 CD cord-351964-hduv0ur4 240 5 B b NN cord-351964-hduv0ur4 240 6 ) ) -RRB- cord-351964-hduv0ur4 240 7 . . . cord-351964-hduv0ur4 241 1 This this DT cord-351964-hduv0ur4 241 2 third third JJ cord-351964-hduv0ur4 241 3 class class NN cord-351964-hduv0ur4 241 4 comprised comprise VBN cord-351964-hduv0ur4 241 5 considerably considerably RB cord-351964-hduv0ur4 241 6 < < XX cord-351964-hduv0ur4 241 7 1 1 CD cord-351964-hduv0ur4 241 8 % % NN cord-351964-hduv0ur4 241 9 of of IN cord-351964-hduv0ur4 241 10 the the DT cord-351964-hduv0ur4 241 11 total total JJ cord-351964-hduv0ur4 241 12 post post JJ cord-351964-hduv0ur4 241 13 - - JJ cord-351964-hduv0ur4 241 14 Golgi golgi JJ cord-351964-hduv0ur4 241 15 vesicles vesicle NNS cord-351964-hduv0ur4 241 16 containing contain VBG cord-351964-hduv0ur4 241 17 mature mature JJ cord-351964-hduv0ur4 241 18 virions virion NNS cord-351964-hduv0ur4 241 19 . . . cord-351964-hduv0ur4 242 1 The the DT cord-351964-hduv0ur4 242 2 fact fact NN cord-351964-hduv0ur4 242 3 that that IN cord-351964-hduv0ur4 242 4 we -PRON- PRP cord-351964-hduv0ur4 242 5 have have VBP cord-351964-hduv0ur4 242 6 never never RB cord-351964-hduv0ur4 242 7 observed observe VBN cord-351964-hduv0ur4 242 8 post post JJ cord-351964-hduv0ur4 242 9 - - JJ cord-351964-hduv0ur4 242 10 Golgi golgi JJ cord-351964-hduv0ur4 242 11 vesicles vesicle NNS cord-351964-hduv0ur4 242 12 containing contain VBG cord-351964-hduv0ur4 242 13 virions virion NNS cord-351964-hduv0ur4 242 14 and and CC cord-351964-hduv0ur4 242 15 aggregates aggregate NNS cord-351964-hduv0ur4 242 16 of of IN cord-351964-hduv0ur4 242 17 condensed condense VBN cord-351964-hduv0ur4 242 18 secretory secretory JJ cord-351964-hduv0ur4 242 19 proteins protein NNS cord-351964-hduv0ur4 242 20 in in IN cord-351964-hduv0ur4 242 21 thin thin JJ cord-351964-hduv0ur4 242 22 sections section NNS cord-351964-hduv0ur4 242 23 of of IN cord-351964-hduv0ur4 242 24 conventionally conventionally RB cord-351964-hduv0ur4 242 25 fixed fix VBN cord-351964-hduv0ur4 242 26 and and CC cord-351964-hduv0ur4 242 27 embedded embed VBN cord-351964-hduv0ur4 242 28 cells cell NNS cord-351964-hduv0ur4 242 29 is be VBZ cord-351964-hduv0ur4 242 30 further further JJ cord-351964-hduv0ur4 242 31 evidence evidence NN cord-351964-hduv0ur4 242 32 of of IN cord-351964-hduv0ur4 242 33 their -PRON- PRP$ cord-351964-hduv0ur4 242 34 rarity rarity NN cord-351964-hduv0ur4 242 35 . . . cord-351964-hduv0ur4 243 1 Their -PRON- PRP$ cord-351964-hduv0ur4 243 2 detection detection NN cord-351964-hduv0ur4 243 3 is be VBZ cord-351964-hduv0ur4 243 4 dependent dependent JJ cord-351964-hduv0ur4 243 5 upon upon IN cord-351964-hduv0ur4 243 6 labeling label VBG cord-351964-hduv0ur4 243 7 the the DT cord-351964-hduv0ur4 243 8 cells cell NNS cord-351964-hduv0ur4 243 9 for for IN cord-351964-hduv0ur4 243 10 ACTH ACTH NNP cord-351964-hduv0ur4 243 11 by by IN cord-351964-hduv0ur4 243 12 the the DT cord-351964-hduv0ur4 243 13 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 243 14 procedure procedure NN cord-351964-hduv0ur4 243 15 . . . cord-351964-hduv0ur4 244 1 The the DT cord-351964-hduv0ur4 244 2 extreme extreme JJ cord-351964-hduv0ur4 244 3 trans trans JJ cord-351964-hduv0ur4 244 4 - - JJ cord-351964-hduv0ur4 244 5 Golgi Golgi NNP cord-351964-hduv0ur4 244 6 cisterna cisterna NNP cord-351964-hduv0ur4 244 7 is be VBZ cord-351964-hduv0ur4 244 8 the the DT cord-351964-hduv0ur4 244 9 most most RBS cord-351964-hduv0ur4 244 10 acidic acidic JJ cord-351964-hduv0ur4 244 11 compartment compartment NN cord-351964-hduv0ur4 244 12 of of IN cord-351964-hduv0ur4 244 13 the the DT cord-351964-hduv0ur4 244 14 Golgi Golgi NNP cord-351964-hduv0ur4 244 15 apparatus apparatus NN cord-351964-hduv0ur4 244 16 ( ( -LRB- cord-351964-hduv0ur4 244 17 Anderson Anderson NNP cord-351964-hduv0ur4 244 18 and and CC cord-351964-hduv0ur4 244 19 Pathak Pathak NNP cord-351964-hduv0ur4 244 20 , , , cord-351964-hduv0ur4 244 21 1985 1985 CD cord-351964-hduv0ur4 244 22 ) ) -RRB- cord-351964-hduv0ur4 244 23 , , , cord-351964-hduv0ur4 244 24 but but CC cord-351964-hduv0ur4 244 25 its -PRON- PRP$ cord-351964-hduv0ur4 244 26 pH ph NN cord-351964-hduv0ur4 244 27 is be VBZ cord-351964-hduv0ur4 244 28 believed believe VBN cord-351964-hduv0ur4 244 29 for for IN cord-351964-hduv0ur4 244 30 a a DT cord-351964-hduv0ur4 244 31 variety variety NN cord-351964-hduv0ur4 244 32 of of IN cord-351964-hduv0ur4 244 33 reasons reason NNS cord-351964-hduv0ur4 244 34 not not RB cord-351964-hduv0ur4 244 35 to to TO cord-351964-hduv0ur4 244 36 be be VB cord-351964-hduv0ur4 244 37 below below IN cord-351964-hduv0ur4 244 38 6 6 CD cord-351964-hduv0ur4 245 1 ( ( -LRB- cord-351964-hduv0ur4 245 2 Anderson Anderson NNP cord-351964-hduv0ur4 245 3 and and CC cord-351964-hduv0ur4 245 4 Pathak Pathak NNP cord-351964-hduv0ur4 245 5 , , , cord-351964-hduv0ur4 245 6 1985 1985 CD cord-351964-hduv0ur4 245 7 ; ; : cord-351964-hduv0ur4 245 8 Griffiths Griffiths NNP cord-351964-hduv0ur4 245 9 and and CC cord-351964-hduv0ur4 245 10 Simons Simons NNP cord-351964-hduv0ur4 245 11 , , , cord-351964-hduv0ur4 245 12 1986 1986 CD cord-351964-hduv0ur4 245 13 ) ) -RRB- cord-351964-hduv0ur4 245 14 . . . cord-351964-hduv0ur4 246 1 In in IN cord-351964-hduv0ur4 246 2 AtT20 AtT20 NNP cord-351964-hduv0ur4 246 3 cells cell NNS cord-351964-hduv0ur4 246 4 , , , cord-351964-hduv0ur4 246 5 as as IN cord-351964-hduv0ur4 246 6 we -PRON- PRP cord-351964-hduv0ur4 246 7 have have VBP cord-351964-hduv0ur4 246 8 recently recently RB cord-351964-hduv0ur4 246 9 shown show VBN cord-351964-hduv0ur4 246 10 ( ( -LRB- cord-351964-hduv0ur4 246 11 unpublished unpublished JJ cord-351964-hduv0ur4 246 12 results result NNS cord-351964-hduv0ur4 246 13 ) ) -RRB- cord-351964-hduv0ur4 246 14 , , , cord-351964-hduv0ur4 246 15 the the DT cord-351964-hduv0ur4 246 16 trans trans NNP cord-351964-hduv0ur4 246 17 - - NNP cord-351964-hduv0ur4 246 18 Golgi Golgi NNP cord-351964-hduv0ur4 246 19 network network NN cord-351964-hduv0ur4 246 20 is be VBZ cord-351964-hduv0ur4 246 21 much much RB cord-351964-hduv0ur4 246 22 less less RBR cord-351964-hduv0ur4 246 23 heavily heavily RB cord-351964-hduv0ur4 246 24 labeled label VBN cord-351964-hduv0ur4 246 25 with with IN cord-351964-hduv0ur4 246 26 3-(2,4-dinitroanilino-3"amino 3-(2,4-dinitroanilino-3"amino CD cord-351964-hduv0ur4 246 27 - - HYPH cord-351964-hduv0ur4 246 28 N n CD cord-351964-hduv0ur4 246 29 - - HYPH cord-351964-hduv0ur4 246 30 methyldipropylamine methyldipropylamine NN cord-351964-hduv0ur4 246 31 ( ( -LRB- cord-351964-hduv0ur4 246 32 Anderson Anderson NNP cord-351964-hduv0ur4 246 33 et et NNP cord-351964-hduv0ur4 246 34 al al NNP cord-351964-hduv0ur4 246 35 . . NNP cord-351964-hduv0ur4 246 36 , , , cord-351964-hduv0ur4 246 37 1984 1984 CD cord-351964-hduv0ur4 246 38 ) ) -RRB- cord-351964-hduv0ur4 246 39 than than IN cord-351964-hduv0ur4 246 40 are be VBP cord-351964-hduv0ur4 246 41 the the DT cord-351964-hduv0ur4 246 42 secretory secretory JJ cord-351964-hduv0ur4 246 43 granules granule NNS cord-351964-hduv0ur4 246 44 whose whose WP$ cord-351964-hduv0ur4 246 45 pH ph NN cord-351964-hduv0ur4 246 46 we -PRON- PRP cord-351964-hduv0ur4 246 47 estimate estimate VBP cord-351964-hduv0ur4 246 48 to to TO cord-351964-hduv0ur4 246 49 be be VB cord-351964-hduv0ur4 246 50 between between IN cord-351964-hduv0ur4 246 51 5.5 5.5 CD cord-351964-hduv0ur4 246 52 and and CC cord-351964-hduv0ur4 246 53 6.0 6.0 CD cord-351964-hduv0ur4 246 54 . . . cord-351964-hduv0ur4 247 1 To to TO cord-351964-hduv0ur4 247 2 test test VB cord-351964-hduv0ur4 247 3 whether whether IN cord-351964-hduv0ur4 247 4 the the DT cord-351964-hduv0ur4 247 5 lower low JJR cord-351964-hduv0ur4 247 6 pH ph NN cord-351964-hduv0ur4 247 7 of of IN cord-351964-hduv0ur4 247 8 this this DT cord-351964-hduv0ur4 247 9 trans trans VB cord-351964-hduv0ur4 247 10 - - JJ cord-351964-hduv0ur4 247 11 most most JJS cord-351964-hduv0ur4 247 12 compartment compartment NN cord-351964-hduv0ur4 247 13 of of IN cord-351964-hduv0ur4 247 14 the the DT cord-351964-hduv0ur4 247 15 Golgi Golgi NNP cord-351964-hduv0ur4 247 16 apparatus apparatus NN cord-351964-hduv0ur4 247 17 is be VBZ cord-351964-hduv0ur4 247 18 necessary necessary JJ cord-351964-hduv0ur4 247 19 for for IN cord-351964-hduv0ur4 247 20 the the DT cord-351964-hduv0ur4 247 21 morphological morphological JJ cord-351964-hduv0ur4 247 22 maturation maturation NN cord-351964-hduv0ur4 247 23 of of IN cord-351964-hduv0ur4 247 24 coronavirus coronavirus NN cord-351964-hduv0ur4 247 25 particles particle NNS cord-351964-hduv0ur4 247 26 , , , cord-351964-hduv0ur4 247 27 we -PRON- PRP cord-351964-hduv0ur4 247 28 exposed expose VBD cord-351964-hduv0ur4 247 29 infected infected JJ cord-351964-hduv0ur4 247 30 cultures culture NNS cord-351964-hduv0ur4 247 31 to to IN cord-351964-hduv0ur4 247 32 200 200 CD cord-351964-hduv0ur4 247 33 ttM ttm JJ cord-351964-hduv0ur4 247 34 chloroquine chloroquine NN cord-351964-hduv0ur4 247 35 from from IN cord-351964-hduv0ur4 247 36 7.5 7.5 CD cord-351964-hduv0ur4 247 37 - - HYPH cord-351964-hduv0ur4 247 38 9-h 9-h CD cord-351964-hduv0ur4 247 39 postinfection postinfection NN cord-351964-hduv0ur4 247 40 and and CC cord-351964-hduv0ur4 247 41 then then RB cord-351964-hduv0ur4 247 42 fixed fix VBD cord-351964-hduv0ur4 247 43 and and CC cord-351964-hduv0ur4 247 44 processed process VBD cord-351964-hduv0ur4 247 45 them -PRON- PRP cord-351964-hduv0ur4 247 46 for for IN cord-351964-hduv0ur4 247 47 electron electron NN cord-351964-hduv0ur4 247 48 microscopy microscopy NN cord-351964-hduv0ur4 247 49 . . . cord-351964-hduv0ur4 248 1 As as IN cord-351964-hduv0ur4 248 2 Fig Fig NNP cord-351964-hduv0ur4 248 3 . . . cord-351964-hduv0ur4 248 4 _SP cord-351964-hduv0ur4 249 1 10 10 CD cord-351964-hduv0ur4 249 2 shows show NNS cord-351964-hduv0ur4 249 3 , , , cord-351964-hduv0ur4 249 4 although although IN cord-351964-hduv0ur4 249 5 exposure exposure NN cord-351964-hduv0ur4 249 6 to to IN cord-351964-hduv0ur4 249 7 chloroquine chloroquine NN cord-351964-hduv0ur4 249 8 causes cause VBZ cord-351964-hduv0ur4 249 9 dilation dilation NN cord-351964-hduv0ur4 249 10 of of IN cord-351964-hduv0ur4 249 11 the the DT cord-351964-hduv0ur4 249 12 trans trans NNP cord-351964-hduv0ur4 249 13 - - NNP cord-351964-hduv0ur4 249 14 Golgi Golgi NNP cord-351964-hduv0ur4 249 15 network network NN cord-351964-hduv0ur4 249 16 , , , cord-351964-hduv0ur4 249 17 the the DT cord-351964-hduv0ur4 249 18 virions virion NNS cord-351964-hduv0ur4 249 19 that that WDT cord-351964-hduv0ur4 249 20 accumulated accumulate VBD cord-351964-hduv0ur4 249 21 in in IN cord-351964-hduv0ur4 249 22 this this DT cord-351964-hduv0ur4 249 23 compartment compartment NN cord-351964-hduv0ur4 249 24 in in IN cord-351964-hduv0ur4 249 25 the the DT cord-351964-hduv0ur4 249 26 presence presence NN cord-351964-hduv0ur4 249 27 of of IN cord-351964-hduv0ur4 249 28 this this DT cord-351964-hduv0ur4 249 29 weak weak JJ cord-351964-hduv0ur4 249 30 base base NN cord-351964-hduv0ur4 249 31 were be VBD cord-351964-hduv0ur4 249 32 as as RB cord-351964-hduv0ur4 249 33 compact compact JJ cord-351964-hduv0ur4 249 34 and and CC cord-351964-hduv0ur4 249 35 electron electron NN cord-351964-hduv0ur4 249 36 dense dense JJ cord-351964-hduv0ur4 249 37 as as IN cord-351964-hduv0ur4 249 38 those those DT cord-351964-hduv0ur4 249 39 in in IN cord-351964-hduv0ur4 249 40 untreated untreated JJ cord-351964-hduv0ur4 249 41 cells cell NNS cord-351964-hduv0ur4 249 42 . . . cord-351964-hduv0ur4 250 1 We -PRON- PRP cord-351964-hduv0ur4 250 2 obtained obtain VBD cord-351964-hduv0ur4 250 3 the the DT cord-351964-hduv0ur4 250 4 same same JJ cord-351964-hduv0ur4 250 5 result result NN cord-351964-hduv0ur4 250 6 when when WRB cord-351964-hduv0ur4 250 7 infected infected JJ cord-351964-hduv0ur4 250 8 cultures culture NNS cord-351964-hduv0ur4 250 9 were be VBD cord-351964-hduv0ur4 250 10 exposed expose VBN cord-351964-hduv0ur4 250 11 from from IN cord-351964-hduv0ur4 250 12 7.5 7.5 CD cord-351964-hduv0ur4 250 13 - - HYPH cord-351964-hduv0ur4 250 14 9-h 9-h CD cord-351964-hduv0ur4 250 15 postinfection postinfection NN cord-351964-hduv0ur4 250 16 to to IN cord-351964-hduv0ur4 250 17 10 10 CD cord-351964-hduv0ur4 250 18 mM mm CD cord-351964-hduv0ur4 250 19 NI NI NNP cord-351964-hduv0ur4 250 20 - - HYPH cord-351964-hduv0ur4 250 21 I4C1 I4C1 NNP cord-351964-hduv0ur4 250 22 ( ( -LRB- cord-351964-hduv0ur4 250 23 data datum NNS cord-351964-hduv0ur4 250 24 not not RB cord-351964-hduv0ur4 250 25 shown show VBN cord-351964-hduv0ur4 250 26 ) ) -RRB- cord-351964-hduv0ur4 250 27 . . . cord-351964-hduv0ur4 251 1 It -PRON- PRP cord-351964-hduv0ur4 251 2 is be VBZ cord-351964-hduv0ur4 251 3 also also RB cord-351964-hduv0ur4 251 4 noteworthy noteworthy JJ cord-351964-hduv0ur4 251 5 that that IN cord-351964-hduv0ur4 251 6 200 200 CD cord-351964-hduv0ur4 252 1 ~tM ~tM NNP cord-351964-hduv0ur4 252 2 chloroquine chloroquine NNP cord-351964-hduv0ur4 252 3 did do VBD cord-351964-hduv0ur4 252 4 not not RB cord-351964-hduv0ur4 252 5 inhibit inhibit VB cord-351964-hduv0ur4 252 6 the the DT cord-351964-hduv0ur4 252 7 formation formation NN cord-351964-hduv0ur4 252 8 of of IN cord-351964-hduv0ur4 252 9 the the DT cord-351964-hduv0ur4 252 10 post post JJ cord-351964-hduv0ur4 252 11 - - JJ cord-351964-hduv0ur4 252 12 Golgi golgi JJ cord-351964-hduv0ur4 252 13 vesicles vesicle NNS cord-351964-hduv0ur4 252 14 packed pack VBN cord-351964-hduv0ur4 252 15 with with IN cord-351964-hduv0ur4 252 16 mature mature JJ cord-351964-hduv0ur4 252 17 virus virus NN cord-351964-hduv0ur4 252 18 particles particle NNS cord-351964-hduv0ur4 252 19 that that WDT cord-351964-hduv0ur4 252 20 occur occur VBP cord-351964-hduv0ur4 252 21 close close RB cord-351964-hduv0ur4 252 22 to to IN cord-351964-hduv0ur4 252 23 the the DT cord-351964-hduv0ur4 252 24 plasma plasma NN cord-351964-hduv0ur4 252 25 membrane membrane NN cord-351964-hduv0ur4 252 26 ( ( -LRB- cord-351964-hduv0ur4 252 27 Fig Fig NNP cord-351964-hduv0ur4 252 28 . . . cord-351964-hduv0ur4 252 29 10 10 CD cord-351964-hduv0ur4 252 30 ) ) -RRB- cord-351964-hduv0ur4 252 31 . . . cord-351964-hduv0ur4 253 1 However however RB cord-351964-hduv0ur4 253 2 , , , cord-351964-hduv0ur4 253 3 in in IN cord-351964-hduv0ur4 253 4 both both CC cord-351964-hduv0ur4 253 5 infected infected JJ cord-351964-hduv0ur4 253 6 and and CC cord-351964-hduv0ur4 253 7 uninfected uninfected JJ cord-351964-hduv0ur4 253 8 cells cell NNS cord-351964-hduv0ur4 253 9 in in IN cord-351964-hduv0ur4 253 10 the the DT cord-351964-hduv0ur4 253 11 same same JJ cord-351964-hduv0ur4 253 12 cultures culture NNS cord-351964-hduv0ur4 253 13 exposed expose VBN cord-351964-hduv0ur4 253 14 for for IN cord-351964-hduv0ur4 253 15 2.5 2.5 CD cord-351964-hduv0ur4 253 16 h h NN cord-351964-hduv0ur4 253 17 to to IN cord-351964-hduv0ur4 253 18 either either CC cord-351964-hduv0ur4 253 19 200 200 CD cord-351964-hduv0ur4 253 20 ttM ttm JJ cord-351964-hduv0ur4 253 21 chloroquine chloroquine NN cord-351964-hduv0ur4 253 22 or or CC cord-351964-hduv0ur4 253 23 1D 1D NNP cord-351964-hduv0ur4 253 24 mM mm CD cord-351964-hduv0ur4 253 25 NI NI NNP cord-351964-hduv0ur4 253 26 - - HYPH cord-351964-hduv0ur4 253 27 I4C1 I4C1 NNP cord-351964-hduv0ur4 254 1 there there EX cord-351964-hduv0ur4 254 2 was be VBD cord-351964-hduv0ur4 254 3 little little JJ cord-351964-hduv0ur4 254 4 if if IN cord-351964-hduv0ur4 254 5 any any DT cord-351964-hduv0ur4 254 6 condensing condense VBG cord-351964-hduv0ur4 254 7 secretory secretory JJ cord-351964-hduv0ur4 254 8 protein protein NN cord-351964-hduv0ur4 254 9 in in IN cord-351964-hduv0ur4 254 10 the the DT cord-351964-hduv0ur4 254 11 trans trans NNP cord-351964-hduv0ur4 254 12 - - NNP cord-351964-hduv0ur4 254 13 Golgi Golgi NNP cord-351964-hduv0ur4 254 14 network network NN cord-351964-hduv0ur4 254 15 of of IN cord-351964-hduv0ur4 254 16 the the DT cord-351964-hduv0ur4 254 17 many many JJ cord-351964-hduv0ur4 254 18 sections section NNS cord-351964-hduv0ur4 254 19 we -PRON- PRP cord-351964-hduv0ur4 254 20 examined examine VBD cord-351964-hduv0ur4 254 21 ( ( -LRB- cord-351964-hduv0ur4 254 22 data datum NNS cord-351964-hduv0ur4 254 23 not not RB cord-351964-hduv0ur4 254 24 shown show VBN cord-351964-hduv0ur4 254 25 ) ) -RRB- cord-351964-hduv0ur4 254 26 . . . cord-351964-hduv0ur4 255 1 Exposure exposure NN cord-351964-hduv0ur4 255 2 of of IN cord-351964-hduv0ur4 255 3 the the DT cord-351964-hduv0ur4 255 4 cells cell NNS cord-351964-hduv0ur4 255 5 to to IN cord-351964-hduv0ur4 255 6 these these DT cord-351964-hduv0ur4 255 7 lysomotrophic lysomotrophic JJ cord-351964-hduv0ur4 255 8 agents agent NNS cord-351964-hduv0ur4 255 9 apparently apparently RB cord-351964-hduv0ur4 255 10 , , , cord-351964-hduv0ur4 255 11 therefore therefore RB cord-351964-hduv0ur4 255 12 , , , cord-351964-hduv0ur4 255 13 had have VBD cord-351964-hduv0ur4 255 14 a a DT cord-351964-hduv0ur4 255 15 differential differential JJ cord-351964-hduv0ur4 255 16 effect effect NN cord-351964-hduv0ur4 255 17 inhibiting inhibit VBG cord-351964-hduv0ur4 255 18 condensation condensation NN cord-351964-hduv0ur4 255 19 of of IN cord-351964-hduv0ur4 255 20 secretory secretory JJ cord-351964-hduv0ur4 255 21 proteins protein NNS cord-351964-hduv0ur4 255 22 but but CC cord-351964-hduv0ur4 255 23 not not RB cord-351964-hduv0ur4 255 24 inhibiting inhibit VBG cord-351964-hduv0ur4 255 25 the the DT cord-351964-hduv0ur4 255 26 morphological morphological JJ cord-351964-hduv0ur4 255 27 maturation maturation NN cord-351964-hduv0ur4 255 28 of of IN cord-351964-hduv0ur4 255 29 the the DT cord-351964-hduv0ur4 255 30 coronavirions coronavirion NNS cord-351964-hduv0ur4 255 31 . . . cord-351964-hduv0ur4 256 1 Viral viral JJ cord-351964-hduv0ur4 256 2 infection infection NN cord-351964-hduv0ur4 256 3 is be VBZ cord-351964-hduv0ur4 256 4 a a DT cord-351964-hduv0ur4 256 5 convenient convenient JJ cord-351964-hduv0ur4 256 6 way way NN cord-351964-hduv0ur4 256 7 in in IN cord-351964-hduv0ur4 256 8 which which WDT cord-351964-hduv0ur4 256 9 to to TO cord-351964-hduv0ur4 256 10 introduce introduce VB cord-351964-hduv0ur4 256 11 foreign foreign JJ cord-351964-hduv0ur4 256 12 genes gene NNS cord-351964-hduv0ur4 256 13 into into IN cord-351964-hduv0ur4 256 14 cells cell NNS cord-351964-hduv0ur4 256 15 to to TO cord-351964-hduv0ur4 256 16 study study VB cord-351964-hduv0ur4 256 17 the the DT cord-351964-hduv0ur4 256 18 synthesis synthesis NN cord-351964-hduv0ur4 256 19 , , , cord-351964-hduv0ur4 256 20 intracellular intracellular JJ cord-351964-hduv0ur4 256 21 transport transport NN cord-351964-hduv0ur4 256 22 , , , cord-351964-hduv0ur4 256 23 and and CC cord-351964-hduv0ur4 256 24 sorting sorting NN cord-351964-hduv0ur4 256 25 of of IN cord-351964-hduv0ur4 256 26 foreign foreign JJ cord-351964-hduv0ur4 256 27 proteins protein NNS cord-351964-hduv0ur4 256 28 . . . cord-351964-hduv0ur4 257 1 The the DT cord-351964-hduv0ur4 257 2 approach approach NN cord-351964-hduv0ur4 257 3 has have VBZ cord-351964-hduv0ur4 257 4 one one CD cord-351964-hduv0ur4 257 5 chief chief JJ cord-351964-hduv0ur4 257 6 disadvantage disadvantage NN cord-351964-hduv0ur4 257 7 , , , cord-351964-hduv0ur4 257 8 namely namely RB cord-351964-hduv0ur4 257 9 the the DT cord-351964-hduv0ur4 257 10 cytopathic cytopathic JJ cord-351964-hduv0ur4 257 11 effects effect NNS cord-351964-hduv0ur4 257 12 that that WDT cord-351964-hduv0ur4 257 13 accompany accompany VBP cord-351964-hduv0ur4 257 14 viral viral JJ cord-351964-hduv0ur4 257 15 replication replication NN cord-351964-hduv0ur4 257 16 . . . cord-351964-hduv0ur4 258 1 This this DT cord-351964-hduv0ur4 258 2 problem problem NN cord-351964-hduv0ur4 258 3 can can MD cord-351964-hduv0ur4 258 4 , , , cord-351964-hduv0ur4 258 5 however however RB cord-351964-hduv0ur4 258 6 , , , cord-351964-hduv0ur4 258 7 be be VB cord-351964-hduv0ur4 258 8 largely largely RB cord-351964-hduv0ur4 258 9 avoided avoid VBN cord-351964-hduv0ur4 258 10 by by IN cord-351964-hduv0ur4 258 11 studying study VBG cord-351964-hduv0ur4 258 12 the the DT cord-351964-hduv0ur4 258 13 cells cell NNS cord-351964-hduv0ur4 258 14 early early RB cord-351964-hduv0ur4 258 15 after after IN cord-351964-hduv0ur4 258 16 infection infection NN cord-351964-hduv0ur4 258 17 before before IN cord-351964-hduv0ur4 258 18 the the DT cord-351964-hduv0ur4 258 19 onset onset NN cord-351964-hduv0ur4 258 20 of of IN cord-351964-hduv0ur4 258 21 the the DT cord-351964-hduv0ur4 258 22 cytopathic cytopathic JJ cord-351964-hduv0ur4 258 23 changes change NNS cord-351964-hduv0ur4 258 24 . . . cord-351964-hduv0ur4 259 1 In in IN cord-351964-hduv0ur4 259 2 the the DT cord-351964-hduv0ur4 259 3 case case NN cord-351964-hduv0ur4 259 4 of of IN cord-351964-hduv0ur4 259 5 AtT20 AtT20 NNP cord-351964-hduv0ur4 259 6 ceils ceils NNP cord-351964-hduv0ur4 259 7 infected infect VBN cord-351964-hduv0ur4 259 8 by by IN cord-351964-hduv0ur4 259 9 MHV MHV NNP cord-351964-hduv0ur4 259 10 - - HYPH cord-351964-hduv0ur4 259 11 A59 A59 NNP cord-351964-hduv0ur4 260 1 we -PRON- PRP cord-351964-hduv0ur4 260 2 believe believe VBP cord-351964-hduv0ur4 260 3 that that IN cord-351964-hduv0ur4 260 4 at at IN cord-351964-hduv0ur4 260 5 7 7 CD cord-351964-hduv0ur4 260 6 - - SYM cord-351964-hduv0ur4 260 7 10-h 10-h CD cord-351964-hduv0ur4 260 8 postinfection postinfection NN cord-351964-hduv0ur4 260 9 the the DT cord-351964-hduv0ur4 260 10 exocytic exocytic JJ cord-351964-hduv0ur4 260 11 pathways pathway NNS cord-351964-hduv0ur4 260 12 , , , cord-351964-hduv0ur4 260 13 and and CC cord-351964-hduv0ur4 260 14 in in IN cord-351964-hduv0ur4 260 15 particular particular JJ cord-351964-hduv0ur4 260 16 the the DT cord-351964-hduv0ur4 260 17 Golgi Golgi NNP cord-351964-hduv0ur4 260 18 apparatus apparatus NN cord-351964-hduv0ur4 260 19 , , , cord-351964-hduv0ur4 260 20 still still RB cord-351964-hduv0ur4 260 21 function function VB cord-351964-hduv0ur4 260 22 normally normally RB cord-351964-hduv0ur4 260 23 for for IN cord-351964-hduv0ur4 260 24 the the DT cord-351964-hduv0ur4 260 25 following follow VBG cord-351964-hduv0ur4 260 26 reasons reason NNS cord-351964-hduv0ur4 260 27 : : : cord-351964-hduv0ur4 260 28 ( ( -LRB- cord-351964-hduv0ur4 260 29 a a LS cord-351964-hduv0ur4 260 30 ) ) -RRB- cord-351964-hduv0ur4 261 1 The the DT cord-351964-hduv0ur4 261 2 Golgi Golgi NNP cord-351964-hduv0ur4 261 3 stack stack NN cord-351964-hduv0ur4 261 4 has have VBZ cord-351964-hduv0ur4 261 5 a a DT cord-351964-hduv0ur4 261 6 normal normal JJ cord-351964-hduv0ur4 261 7 morphology morphology NN cord-351964-hduv0ur4 261 8 . . . cord-351964-hduv0ur4 262 1 ( ( -LRB- cord-351964-hduv0ur4 262 2 b b LS cord-351964-hduv0ur4 262 3 ) ) -RRB- cord-351964-hduv0ur4 262 4 The the DT cord-351964-hduv0ur4 262 5 Golgi Golgi NNP cord-351964-hduv0ur4 262 6 enzymes enzyme NNS cord-351964-hduv0ur4 262 7 responsible responsible JJ cord-351964-hduv0ur4 262 8 for for IN cord-351964-hduv0ur4 262 9 O o NN cord-351964-hduv0ur4 262 10 - - HYPH cord-351964-hduv0ur4 262 11 linked link VBN cord-351964-hduv0ur4 262 12 glycosylation glycosylation NN cord-351964-hduv0ur4 262 13 are be VBP cord-351964-hduv0ur4 262 14 functional functional JJ cord-351964-hduv0ur4 262 15 . . . cord-351964-hduv0ur4 263 1 ( ( -LRB- cord-351964-hduv0ur4 263 2 c c NN cord-351964-hduv0ur4 263 3 ) ) -RRB- cord-351964-hduv0ur4 263 4 VSV VSV NNP cord-351964-hduv0ur4 263 5 - - HYPH cord-351964-hduv0ur4 263 6 G G NNP cord-351964-hduv0ur4 263 7 protein protein NN cord-351964-hduv0ur4 263 8 is be VBZ cord-351964-hduv0ur4 263 9 N n CD cord-351964-hduv0ur4 263 10 - - HYPH cord-351964-hduv0ur4 263 11 glycosylated glycosylate VBN cord-351964-hduv0ur4 263 12 in in IN cord-351964-hduv0ur4 263 13 the the DT cord-351964-hduv0ur4 263 14 same same JJ cord-351964-hduv0ur4 263 15 way way NN cord-351964-hduv0ur4 263 16 in in IN cord-351964-hduv0ur4 263 17 both both CC cord-351964-hduv0ur4 263 18 uninfected uninfected JJ cord-351964-hduv0ur4 263 19 and and CC cord-351964-hduv0ur4 263 20 MHV MHV NNP cord-351964-hduv0ur4 263 21 - - HYPH cord-351964-hduv0ur4 263 22 A59-infected A59-infected NNP cord-351964-hduv0ur4 263 23 AtT20 AtT20 NNP cord-351964-hduv0ur4 263 24 cells cell NNS cord-351964-hduv0ur4 263 25 . . . cord-351964-hduv0ur4 264 1 Furthermore furthermore RB cord-351964-hduv0ur4 264 2 the the DT cord-351964-hduv0ur4 264 3 G g NN cord-351964-hduv0ur4 264 4 protein protein NN cord-351964-hduv0ur4 264 5 is be VBZ cord-351964-hduv0ur4 264 6 transported transport VBN cord-351964-hduv0ur4 264 7 to to IN cord-351964-hduv0ur4 264 8 the the DT cord-351964-hduv0ur4 264 9 plasma plasma NN cord-351964-hduv0ur4 264 10 membrane membrane NN cord-351964-hduv0ur4 264 11 in in IN cord-351964-hduv0ur4 264 12 both both DT cord-351964-hduv0ur4 264 13 uninfected uninfected JJ cord-351964-hduv0ur4 264 14 and and CC cord-351964-hduv0ur4 264 15 MHV MHV NNP cord-351964-hduv0ur4 264 16 - - HYPH cord-351964-hduv0ur4 264 17 A59-infected a59-infecte VBN cord-351964-hduv0ur4 264 18 cells cell NNS cord-351964-hduv0ur4 264 19 . . . cord-351964-hduv0ur4 265 1 Therefore therefore RB cord-351964-hduv0ur4 265 2 the the DT cord-351964-hduv0ur4 265 3 constitutive constitutive JJ cord-351964-hduv0ur4 265 4 exocytic exocytic JJ cord-351964-hduv0ur4 265 5 pathway pathway NN cord-351964-hduv0ur4 265 6 is be VBZ cord-351964-hduv0ur4 265 7 fully fully RB cord-351964-hduv0ur4 265 8 functional functional JJ cord-351964-hduv0ur4 265 9 after after IN cord-351964-hduv0ur4 265 10 infection infection NN cord-351964-hduv0ur4 265 11 with with IN cord-351964-hduv0ur4 265 12 the the DT cord-351964-hduv0ur4 265 13 coronavirus coronavirus NN cord-351964-hduv0ur4 265 14 . . . cord-351964-hduv0ur4 266 1 ( ( -LRB- cord-351964-hduv0ur4 266 2 d d LS cord-351964-hduv0ur4 266 3 ) ) -RRB- cord-351964-hduv0ur4 266 4 In in IN cord-351964-hduv0ur4 266 5 infected infected JJ cord-351964-hduv0ur4 266 6 cells cell NNS cord-351964-hduv0ur4 266 7 , , , cord-351964-hduv0ur4 266 8 as as IN cord-351964-hduv0ur4 266 9 in in IN cord-351964-hduv0ur4 266 10 uninfected uninfected JJ cord-351964-hduv0ur4 266 11 cells cell NNS cord-351964-hduv0ur4 266 12 , , , cord-351964-hduv0ur4 266 13 the the DT cord-351964-hduv0ur4 266 14 first first JJ cord-351964-hduv0ur4 266 15 compartment compartment NN cord-351964-hduv0ur4 266 16 in in IN cord-351964-hduv0ur4 266 17 which which WDT cord-351964-hduv0ur4 266 18 condensed condense VBN cord-351964-hduv0ur4 266 19 secretory secretory JJ cord-351964-hduv0ur4 266 20 proteins protein NNS cord-351964-hduv0ur4 266 21 occur occur VBP cord-351964-hduv0ur4 266 22 is be VBZ cord-351964-hduv0ur4 266 23 the the DT cord-351964-hduv0ur4 266 24 trans trans NNP cord-351964-hduv0ur4 266 25 - - NNP cord-351964-hduv0ur4 266 26 Golgi Golgi NNP cord-351964-hduv0ur4 266 27 network network NN cord-351964-hduv0ur4 266 28 , , , cord-351964-hduv0ur4 266 29 indicating indicate VBG cord-351964-hduv0ur4 266 30 that that IN cord-351964-hduv0ur4 266 31 secretory secretory JJ cord-351964-hduv0ur4 266 32 proteins protein NNS cord-351964-hduv0ur4 266 33 of of IN cord-351964-hduv0ur4 266 34 the the DT cord-351964-hduv0ur4 266 35 regulated regulated JJ cord-351964-hduv0ur4 266 36 exocytic exocytic JJ cord-351964-hduv0ur4 266 37 pathway pathway NN cord-351964-hduv0ur4 266 38 are be VBP cord-351964-hduv0ur4 266 39 being be VBG cord-351964-hduv0ur4 266 40 synthesized synthesize VBN cord-351964-hduv0ur4 266 41 , , , cord-351964-hduv0ur4 266 42 transported transport VBN cord-351964-hduv0ur4 266 43 , , , cord-351964-hduv0ur4 266 44 and and CC cord-351964-hduv0ur4 266 45 condensed condense VBN cord-351964-hduv0ur4 266 46 normally normally RB cord-351964-hduv0ur4 266 47 . . . cord-351964-hduv0ur4 267 1 ( ( -LRB- cord-351964-hduv0ur4 267 2 e e LS cord-351964-hduv0ur4 267 3 ) ) -RRB- cord-351964-hduv0ur4 268 1 The the DT cord-351964-hduv0ur4 268 2 E1 e1 NN cord-351964-hduv0ur4 268 3 envelope envelope NN cord-351964-hduv0ur4 268 4 glycoprotein glycoprotein NN cord-351964-hduv0ur4 268 5 of of IN cord-351964-hduv0ur4 268 6 the the DT cord-351964-hduv0ur4 268 7 virus virus NN cord-351964-hduv0ur4 268 8 is be VBZ cord-351964-hduv0ur4 268 9 not not RB cord-351964-hduv0ur4 268 10 transported transport VBN cord-351964-hduv0ur4 268 11 beyond beyond IN cord-351964-hduv0ur4 268 12 the the DT cord-351964-hduv0ur4 268 13 Golgi Golgi NNP cord-351964-hduv0ur4 268 14 stack stack NN cord-351964-hduv0ur4 268 15 , , , cord-351964-hduv0ur4 268 16 except except IN cord-351964-hduv0ur4 268 17 as as IN cord-351964-hduv0ur4 268 18 an an DT cord-351964-hduv0ur4 268 19 integral integral JJ cord-351964-hduv0ur4 268 20 component component NN cord-351964-hduv0ur4 268 21 of of IN cord-351964-hduv0ur4 268 22 the the DT cord-351964-hduv0ur4 268 23 envelopes envelope NNS cord-351964-hduv0ur4 268 24 of of IN cord-351964-hduv0ur4 268 25 progeny progeny JJ cord-351964-hduv0ur4 268 26 virions virion NNS cord-351964-hduv0ur4 268 27 ( ( -LRB- cord-351964-hduv0ur4 268 28 Fig Fig NNP cord-351964-hduv0ur4 268 29 . . . cord-351964-hduv0ur4 268 30 6 6 CD cord-351964-hduv0ur4 268 31 ) ) -RRB- cord-351964-hduv0ur4 268 32 . . . cord-351964-hduv0ur4 269 1 By by IN cord-351964-hduv0ur4 269 2 contrast contrast NN cord-351964-hduv0ur4 269 3 the the DT cord-351964-hduv0ur4 269 4 second second JJ cord-351964-hduv0ur4 269 5 viral viral JJ cord-351964-hduv0ur4 269 6 envelope envelope NN cord-351964-hduv0ur4 269 7 glycoprotein glycoprotein NN cord-351964-hduv0ur4 269 8 E2 E2 NNP cord-351964-hduv0ur4 269 9 is be VBZ cord-351964-hduv0ur4 269 10 transported transport VBN cord-351964-hduv0ur4 269 11 to to IN cord-351964-hduv0ur4 269 12 the the DT cord-351964-hduv0ur4 269 13 cell cell NN cord-351964-hduv0ur4 269 14 surface surface NN cord-351964-hduv0ur4 269 15 , , , cord-351964-hduv0ur4 269 16 as as IN cord-351964-hduv0ur4 269 17 we -PRON- PRP cord-351964-hduv0ur4 269 18 have have VBP cord-351964-hduv0ur4 269 19 previously previously RB cord-351964-hduv0ur4 269 20 shown show VBN cord-351964-hduv0ur4 269 21 ( ( -LRB- cord-351964-hduv0ur4 269 22 Tooze Tooze NNP cord-351964-hduv0ur4 269 23 and and CC cord-351964-hduv0ur4 269 24 Tooze Tooze NNP cord-351964-hduv0ur4 269 25 , , , cord-351964-hduv0ur4 269 26 1985 1985 CD cord-351964-hduv0ur4 269 27 ) ) -RRB- cord-351964-hduv0ur4 269 28 . . . cord-351964-hduv0ur4 270 1 Therefore therefore RB cord-351964-hduv0ur4 270 2 the the DT cord-351964-hduv0ur4 270 3 Golgi Golgi NNP cord-351964-hduv0ur4 270 4 apparatus apparatus NN cord-351964-hduv0ur4 270 5 in in IN cord-351964-hduv0ur4 270 6 MI MI NNP cord-351964-hduv0ur4 270 7 - - HYPH cord-351964-hduv0ur4 270 8 IV IV NNP cord-351964-hduv0ur4 270 9 - - HYPH cord-351964-hduv0ur4 270 10 A59-infected a59-infecte VBN cord-351964-hduv0ur4 270 11 ceils ceil NNS cord-351964-hduv0ur4 270 12 at at IN cord-351964-hduv0ur4 270 13 7 7 CD cord-351964-hduv0ur4 270 14 - - SYM cord-351964-hduv0ur4 270 15 10-h 10-h CD cord-351964-hduv0ur4 270 16 postinfection postinfection NN cord-351964-hduv0ur4 270 17 can can MD cord-351964-hduv0ur4 270 18 sort sort VB cord-351964-hduv0ur4 270 19 one one CD cord-351964-hduv0ur4 270 20 coronaviral coronaviral JJ cord-351964-hduv0ur4 270 21 glycoprotein glycoprotein NN cord-351964-hduv0ur4 270 22 from from IN cord-351964-hduv0ur4 270 23 the the DT cord-351964-hduv0ur4 270 24 other other JJ cord-351964-hduv0ur4 270 25 . . . cord-351964-hduv0ur4 271 1 By by IN cord-351964-hduv0ur4 271 2 these these DT cord-351964-hduv0ur4 271 3 five five CD cord-351964-hduv0ur4 271 4 criteria criterion NNS cord-351964-hduv0ur4 271 5 the the DT cord-351964-hduv0ur4 271 6 Golgi Golgi NNP cord-351964-hduv0ur4 271 7 apparatus apparatus NN cord-351964-hduv0ur4 271 8 maintains maintain VBZ cord-351964-hduv0ur4 271 9 its -PRON- PRP$ cord-351964-hduv0ur4 271 10 structural structural JJ cord-351964-hduv0ur4 271 11 and and CC cord-351964-hduv0ur4 271 12 functional functional JJ cord-351964-hduv0ur4 271 13 integrity integrity NN cord-351964-hduv0ur4 271 14 until until IN cord-351964-hduv0ur4 271 15 at at RB cord-351964-hduv0ur4 271 16 least least JJS cord-351964-hduv0ur4 271 17 10-h 10-h CD cord-351964-hduv0ur4 271 18 postinfection postinfection NN cord-351964-hduv0ur4 271 19 with with IN cord-351964-hduv0ur4 271 20 MHV MHV NNP cord-351964-hduv0ur4 271 21 - - HYPH cord-351964-hduv0ur4 271 22 A59 A59 NNP cord-351964-hduv0ur4 271 23 . . . cord-351964-hduv0ur4 272 1 In in IN cord-351964-hduv0ur4 272 2 this this DT cord-351964-hduv0ur4 272 3 context context NN cord-351964-hduv0ur4 272 4 it -PRON- PRP cord-351964-hduv0ur4 272 5 is be VBZ cord-351964-hduv0ur4 272 6 interesting interesting JJ cord-351964-hduv0ur4 272 7 to to TO cord-351964-hduv0ur4 272 8 note note VB cord-351964-hduv0ur4 272 9 that that IN cord-351964-hduv0ur4 272 10 in in IN cord-351964-hduv0ur4 272 11 cells cell NNS cord-351964-hduv0ur4 272 12 infected infect VBN cord-351964-hduv0ur4 272 13 with with IN cord-351964-hduv0ur4 272 14 Uukuniemi Uukuniemi NNP cord-351964-hduv0ur4 272 15 virus virus NN cord-351964-hduv0ur4 272 16 ( ( -LRB- cord-351964-hduv0ur4 272 17 a a DT cord-351964-hduv0ur4 272 18 bunyavirus bunyavirus NNP cord-351964-hduv0ur4 272 19 ) ) -RRB- cord-351964-hduv0ur4 273 1 the the DT cord-351964-hduv0ur4 273 2 constitutive constitutive JJ cord-351964-hduv0ur4 273 3 exocytic exocytic JJ cord-351964-hduv0ur4 273 4 pathway pathway NN cord-351964-hduv0ur4 273 5 also also RB cord-351964-hduv0ur4 273 6 continues continue VBZ cord-351964-hduv0ur4 273 7 to to TO cord-351964-hduv0ur4 273 8 transport transport VB cord-351964-hduv0ur4 273 9 Semliki Semliki NNP cord-351964-hduv0ur4 273 10 Forest Forest NNP cord-351964-hduv0ur4 273 11 virus virus NN cord-351964-hduv0ur4 273 12 glycoprotein glycoprotein NN cord-351964-hduv0ur4 273 13 to to IN cord-351964-hduv0ur4 273 14 the the DT cord-351964-hduv0ur4 273 15 plasma plasma NN cord-351964-hduv0ur4 273 16 membrane membrane NN cord-351964-hduv0ur4 273 17 even even RB cord-351964-hduv0ur4 273 18 after after IN cord-351964-hduv0ur4 273 19 the the DT cord-351964-hduv0ur4 273 20 Golgi Golgi NNP cord-351964-hduv0ur4 273 21 stack stack NN cord-351964-hduv0ur4 273 22 has have VBZ cord-351964-hduv0ur4 273 23 vacuolized vacuolize VBN cord-351964-hduv0ur4 273 24 as as IN cord-351964-hduv0ur4 273 25 a a DT cord-351964-hduv0ur4 273 26 result result NN cord-351964-hduv0ur4 273 27 of of IN cord-351964-hduv0ur4 273 28 the the DT cord-351964-hduv0ur4 273 29 Uukuniemi Uukuniemi NNP cord-351964-hduv0ur4 273 30 virus virus NN cord-351964-hduv0ur4 273 31 infection infection NN cord-351964-hduv0ur4 273 32 ( ( -LRB- cord-351964-hduv0ur4 273 33 Gahmberg Gahmberg NNP cord-351964-hduv0ur4 273 34 et et FW cord-351964-hduv0ur4 273 35 al al NNP cord-351964-hduv0ur4 273 36 . . NNP cord-351964-hduv0ur4 273 37 , , , cord-351964-hduv0ur4 273 38 1986 1986 CD cord-351964-hduv0ur4 273 39 ) ) -RRB- cord-351964-hduv0ur4 273 40 . . . cord-351964-hduv0ur4 274 1 In in IN cord-351964-hduv0ur4 274 2 this this DT cord-351964-hduv0ur4 274 3 case case NN cord-351964-hduv0ur4 274 4 the the DT cord-351964-hduv0ur4 274 5 biochemical biochemical JJ cord-351964-hduv0ur4 274 6 integrity integrity NN cord-351964-hduv0ur4 274 7 of of IN cord-351964-hduv0ur4 274 8 the the DT cord-351964-hduv0ur4 274 9 Golgi Golgi NNP cord-351964-hduv0ur4 274 10 complex complex NN cord-351964-hduv0ur4 274 11 survives survive VBZ cord-351964-hduv0ur4 274 12 longer long RBR cord-351964-hduv0ur4 274 13 than than IN cord-351964-hduv0ur4 274 14 its -PRON- PRP$ cord-351964-hduv0ur4 274 15 morphological morphological JJ cord-351964-hduv0ur4 274 16 integrity integrity NN cord-351964-hduv0ur4 274 17 . . . cord-351964-hduv0ur4 275 1 Unlike unlike IN cord-351964-hduv0ur4 275 2 many many JJ cord-351964-hduv0ur4 275 3 other other JJ cord-351964-hduv0ur4 275 4 viruses virus NNS cord-351964-hduv0ur4 275 5 , , , cord-351964-hduv0ur4 275 6 MHV MHV NNP cord-351964-hduv0ur4 275 7 - - HYPH cord-351964-hduv0ur4 275 8 A59 A59 NNP cord-351964-hduv0ur4 275 9 does do VBZ cord-351964-hduv0ur4 275 10 not not RB cord-351964-hduv0ur4 275 11 , , , cord-351964-hduv0ur4 275 12 early early RB cord-351964-hduv0ur4 275 13 in in IN cord-351964-hduv0ur4 275 14 infection infection NN cord-351964-hduv0ur4 275 15 , , , cord-351964-hduv0ur4 275 16 efficiently efficiently RB cord-351964-hduv0ur4 275 17 inhibit inhibit VB cord-351964-hduv0ur4 275 18 cellular cellular JJ cord-351964-hduv0ur4 275 19 protein protein NN cord-351964-hduv0ur4 275 20 synthesis synthesis NN cord-351964-hduv0ur4 275 21 . . . cord-351964-hduv0ur4 276 1 There there EX cord-351964-hduv0ur4 276 2 is be VBZ cord-351964-hduv0ur4 276 3 nevertheless nevertheless RB cord-351964-hduv0ur4 276 4 , , , cord-351964-hduv0ur4 276 5 during during IN cord-351964-hduv0ur4 276 6 coronavirus coronavirus NN cord-351964-hduv0ur4 276 7 infections infection NNS cord-351964-hduv0ur4 276 8 , , , cord-351964-hduv0ur4 276 9 a a DT cord-351964-hduv0ur4 276 10 progressive progressive JJ cord-351964-hduv0ur4 276 11 competitive competitive JJ cord-351964-hduv0ur4 276 12 inhibition inhibition NN cord-351964-hduv0ur4 276 13 of of IN cord-351964-hduv0ur4 276 14 host host NN cord-351964-hduv0ur4 276 15 - - HYPH cord-351964-hduv0ur4 276 16 protein protein NN cord-351964-hduv0ur4 276 17 synthesis synthesis NN cord-351964-hduv0ur4 276 18 ( ( -LRB- cord-351964-hduv0ur4 276 19 Tooze Tooze NNP cord-351964-hduv0ur4 276 20 , , , cord-351964-hduv0ur4 276 21 S. S. NNP cord-351964-hduv0ur4 276 22 A. a. NN cord-351964-hduv0ur4 276 23 , , , cord-351964-hduv0ur4 276 24 unpublished unpublished JJ cord-351964-hduv0ur4 276 25 data datum NNS cord-351964-hduv0ur4 276 26 ) ) -RRB- cord-351964-hduv0ur4 276 27 . . . cord-351964-hduv0ur4 277 1 Since since IN cord-351964-hduv0ur4 277 2 the the DT cord-351964-hduv0ur4 277 3 early early JJ cord-351964-hduv0ur4 277 4 stages stage NNS cord-351964-hduv0ur4 277 5 of of IN cord-351964-hduv0ur4 277 6 MHV MHV NNP cord-351964-hduv0ur4 277 7 - - HYPH cord-351964-hduv0ur4 277 8 A59 A59 NNP cord-351964-hduv0ur4 277 9 infections infection NNS cord-351964-hduv0ur4 277 10 are be VBP cord-351964-hduv0ur4 277 11 by by IN cord-351964-hduv0ur4 277 12 no no DT cord-351964-hduv0ur4 277 13 means mean NNS cord-351964-hduv0ur4 277 14 synchronous synchronous JJ cord-351964-hduv0ur4 277 15 , , , cord-351964-hduv0ur4 277 16 in in IN cord-351964-hduv0ur4 277 17 any any DT cord-351964-hduv0ur4 277 18 one one CD cord-351964-hduv0ur4 277 19 culture culture NN cord-351964-hduv0ur4 277 20 at at IN cord-351964-hduv0ur4 277 21 7 7 CD cord-351964-hduv0ur4 277 22 - - SYM cord-351964-hduv0ur4 277 23 10-h 10-h CD cord-351964-hduv0ur4 277 24 post post NN cord-351964-hduv0ur4 277 25 infection infection NN cord-351964-hduv0ur4 277 26 the the DT cord-351964-hduv0ur4 277 27 extent extent NN cord-351964-hduv0ur4 277 28 of of IN cord-351964-hduv0ur4 277 29 virus virus NN cord-351964-hduv0ur4 277 30 budding budding NN cord-351964-hduv0ur4 277 31 varies vary VBZ cord-351964-hduv0ur4 277 32 considerably considerably RB cord-351964-hduv0ur4 277 33 from from IN cord-351964-hduv0ur4 277 34 infected infected JJ cord-351964-hduv0ur4 277 35 cell cell NN cord-351964-hduv0ur4 277 36 to to IN cord-351964-hduv0ur4 277 37 cell cell NN cord-351964-hduv0ur4 277 38 . . . cord-351964-hduv0ur4 278 1 However however RB cord-351964-hduv0ur4 278 2 , , , cord-351964-hduv0ur4 278 3 in in IN cord-351964-hduv0ur4 278 4 most most JJS cord-351964-hduv0ur4 278 5 in in IN cord-351964-hduv0ur4 278 6 - - HYPH cord-351964-hduv0ur4 278 7 fected fecte VBN cord-351964-hduv0ur4 278 8 cells cell NNS cord-351964-hduv0ur4 278 9 the the DT cord-351964-hduv0ur4 278 10 number number NN cord-351964-hduv0ur4 278 11 of of IN cord-351964-hduv0ur4 278 12 virions virion NNS cord-351964-hduv0ur4 278 13 increases increase NNS cord-351964-hduv0ur4 278 14 rapidly rapidly RB cord-351964-hduv0ur4 278 15 after after IN cord-351964-hduv0ur4 278 16 10 10 CD cord-351964-hduv0ur4 278 17 h h NN cord-351964-hduv0ur4 278 18 of of IN cord-351964-hduv0ur4 278 19 infection infection NN cord-351964-hduv0ur4 278 20 . . . cord-351964-hduv0ur4 279 1 Concomittantly concomittantly RB cord-351964-hduv0ur4 279 2 the the DT cord-351964-hduv0ur4 279 3 number number NN cord-351964-hduv0ur4 279 4 of of IN cord-351964-hduv0ur4 279 5 infected infected JJ cord-351964-hduv0ur4 279 6 cells cell NNS cord-351964-hduv0ur4 279 7 with with IN cord-351964-hduv0ur4 279 8 condensing condense VBG cord-351964-hduv0ur4 279 9 secretory secretory JJ cord-351964-hduv0ur4 279 10 proteins protein NNS cord-351964-hduv0ur4 279 11 in in IN cord-351964-hduv0ur4 279 12 their -PRON- PRP$ cord-351964-hduv0ur4 279 13 trans trans JJ cord-351964-hduv0ur4 279 14 - - JJ cord-351964-hduv0ur4 279 15 Golgi Golgi NNP cord-351964-hduv0ur4 279 16 network network NN cord-351964-hduv0ur4 279 17 rapidly rapidly RB cord-351964-hduv0ur4 279 18 declines decline VBZ cord-351964-hduv0ur4 279 19 and and CC cord-351964-hduv0ur4 279 20 ACTH ACTH NNP cord-351964-hduv0ur4 279 21 can can MD cord-351964-hduv0ur4 279 22 no no RB cord-351964-hduv0ur4 279 23 longer longer RB cord-351964-hduv0ur4 279 24 be be VB cord-351964-hduv0ur4 279 25 detected detect VBN cord-351964-hduv0ur4 279 26 by by IN cord-351964-hduv0ur4 279 27 the the DT cord-351964-hduv0ur4 279 28 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 279 29 method method NN cord-351964-hduv0ur4 279 30 in in IN cord-351964-hduv0ur4 279 31 cisternae cisternae NN cord-351964-hduv0ur4 279 32 of of IN cord-351964-hduv0ur4 279 33 the the DT cord-351964-hduv0ur4 279 34 endoplasmic endoplasmic NN cord-351964-hduv0ur4 279 35 reticulum reticulum NN cord-351964-hduv0ur4 279 36 ( ( -LRB- cord-351964-hduv0ur4 279 37 data datum NNS cord-351964-hduv0ur4 279 38 not not RB cord-351964-hduv0ur4 279 39 shown show VBN cord-351964-hduv0ur4 279 40 ) ) -RRB- cord-351964-hduv0ur4 279 41 . . . cord-351964-hduv0ur4 280 1 Our -PRON- PRP$ cord-351964-hduv0ur4 280 2 observations observation NNS cord-351964-hduv0ur4 280 3 depended depend VBD cord-351964-hduv0ur4 280 4 , , , cord-351964-hduv0ur4 280 5 therefore therefore RB cord-351964-hduv0ur4 280 6 , , , cord-351964-hduv0ur4 280 7 on on IN cord-351964-hduv0ur4 280 8 examining examine VBG cord-351964-hduv0ur4 280 9 the the DT cord-351964-hduv0ur4 280 10 cells cell NNS cord-351964-hduv0ur4 280 11 during during IN cord-351964-hduv0ur4 280 12 the the DT cord-351964-hduv0ur4 280 13 relatively relatively RB cord-351964-hduv0ur4 280 14 short short JJ cord-351964-hduv0ur4 280 15 time time NN cord-351964-hduv0ur4 280 16 after after IN cord-351964-hduv0ur4 280 17 the the DT cord-351964-hduv0ur4 280 18 budding budding NN cord-351964-hduv0ur4 280 19 of of IN cord-351964-hduv0ur4 280 20 progeny progeny JJ cord-351964-hduv0ur4 280 21 virions virion NNS cord-351964-hduv0ur4 280 22 had have VBD cord-351964-hduv0ur4 280 23 begun begin VBN cord-351964-hduv0ur4 280 24 and and CC cord-351964-hduv0ur4 280 25 before before IN cord-351964-hduv0ur4 280 26 cellular cellular JJ cord-351964-hduv0ur4 280 27 protein protein NN cord-351964-hduv0ur4 280 28 synthesis synthesis NN cord-351964-hduv0ur4 280 29 was be VBD cord-351964-hduv0ur4 280 30 inhibited inhibit VBN cord-351964-hduv0ur4 280 31 . . . cord-351964-hduv0ur4 281 1 In in IN cord-351964-hduv0ur4 281 2 the the DT cord-351964-hduv0ur4 281 3 pulse pulse NN cord-351964-hduv0ur4 281 4 - - HYPH cord-351964-hduv0ur4 281 5 chase chase NN cord-351964-hduv0ur4 281 6 experiment experiment NN cord-351964-hduv0ur4 281 7 reported report VBN cord-351964-hduv0ur4 281 8 here here RB cord-351964-hduv0ur4 281 9 , , , cord-351964-hduv0ur4 281 10 two two CD cord-351964-hduv0ur4 281 11 glycosylated glycosylated JJ cord-351964-hduv0ur4 281 12 forms form NNS cord-351964-hduv0ur4 281 13 of of IN cord-351964-hduv0ur4 281 14 the the DT cord-351964-hduv0ur4 281 15 MHV MHV NNP cord-351964-hduv0ur4 281 16 - - HYPH cord-351964-hduv0ur4 281 17 A59 A59 NNP cord-351964-hduv0ur4 281 18 E1 e1 NN cord-351964-hduv0ur4 281 19 glycoprotein glycoprotein NN cord-351964-hduv0ur4 281 20 were be VBD cord-351964-hduv0ur4 281 21 resolved resolve VBN cord-351964-hduv0ur4 281 22 ( ( -LRB- cord-351964-hduv0ur4 281 23 Fig fig NN cord-351964-hduv0ur4 281 24 . . . cord-351964-hduv0ur4 281 25 3 3 CD cord-351964-hduv0ur4 281 26 ) ) -RRB- cord-351964-hduv0ur4 281 27 . . . cord-351964-hduv0ur4 282 1 However however RB cord-351964-hduv0ur4 282 2 , , , cord-351964-hduv0ur4 282 3 the the DT cord-351964-hduv0ur4 282 4 simplicity simplicity NN cord-351964-hduv0ur4 282 5 of of IN cord-351964-hduv0ur4 282 6 the the DT cord-351964-hduv0ur4 282 7 gel gel NN cord-351964-hduv0ur4 282 8 electrophoretic electrophoretic JJ cord-351964-hduv0ur4 282 9 pattern pattern NN cord-351964-hduv0ur4 282 10 of of IN cord-351964-hduv0ur4 282 11 glycosylated glycosylated JJ cord-351964-hduv0ur4 282 12 E1 e1 NN cord-351964-hduv0ur4 282 13 molecules molecule NNS cord-351964-hduv0ur4 282 14 from from IN cord-351964-hduv0ur4 282 15 AtT20 AtT20 NNP cord-351964-hduv0ur4 282 16 cells cell NNS cord-351964-hduv0ur4 282 17 contrasts contrast VBZ cord-351964-hduv0ur4 282 18 with with IN cord-351964-hduv0ur4 282 19 the the DT cord-351964-hduv0ur4 282 20 ladder ladder NN cord-351964-hduv0ur4 282 21 of of IN cord-351964-hduv0ur4 282 22 variously variously RB cord-351964-hduv0ur4 282 23 glycosylated glycosylate VBN cord-351964-hduv0ur4 282 24 molecules molecule NNS cord-351964-hduv0ur4 282 25 obtained obtain VBN cord-351964-hduv0ur4 282 26 by by IN cord-351964-hduv0ur4 282 27 gel gel NN cord-351964-hduv0ur4 282 28 electrophoresis electrophoresis NN cord-351964-hduv0ur4 282 29 of of IN cord-351964-hduv0ur4 282 30 E1 e1 NN cord-351964-hduv0ur4 282 31 isolated isolate VBN cord-351964-hduv0ur4 282 32 from from IN cord-351964-hduv0ur4 282 33 infected infected JJ cord-351964-hduv0ur4 282 34 murine murine JJ cord-351964-hduv0ur4 282 35 fibroblasts fibroblast NNS cord-351964-hduv0ur4 282 36 ( ( -LRB- cord-351964-hduv0ur4 282 37 Niemann Niemann NNP cord-351964-hduv0ur4 282 38 et et NNP cord-351964-hduv0ur4 282 39 al al NNP cord-351964-hduv0ur4 282 40 . . NNP cord-351964-hduv0ur4 282 41 , , , cord-351964-hduv0ur4 282 42 1984 1984 CD cord-351964-hduv0ur4 282 43 ; ; : cord-351964-hduv0ur4 282 44 Tooze Tooze NNP cord-351964-hduv0ur4 282 45 , , , cord-351964-hduv0ur4 282 46 S. S. NNP cord-351964-hduv0ur4 282 47 A. a. NN cord-351964-hduv0ur4 282 48 , , , cord-351964-hduv0ur4 282 49 J. J. NNP cord-351964-hduv0ur4 282 50 Tooze Tooze NNP cord-351964-hduv0ur4 282 51 , , , cord-351964-hduv0ur4 282 52 and and CC cord-351964-hduv0ur4 282 53 G. G. NNP cord-351964-hduv0ur4 282 54 Warren Warren NNP cord-351964-hduv0ur4 282 55 , , , cord-351964-hduv0ur4 282 56 manuscript manuscript NN cord-351964-hduv0ur4 282 57 in in IN cord-351964-hduv0ur4 282 58 preparation preparation NN cord-351964-hduv0ur4 282 59 ) ) -RRB- cord-351964-hduv0ur4 282 60 . . . cord-351964-hduv0ur4 283 1 In in IN cord-351964-hduv0ur4 283 2 murine murine JJ cord-351964-hduv0ur4 283 3 fibroblasts fibroblast NNS cord-351964-hduv0ur4 283 4 the the DT cord-351964-hduv0ur4 283 5 O o NN cord-351964-hduv0ur4 283 6 - - HYPH cord-351964-hduv0ur4 283 7 glycan glycan NN cord-351964-hduv0ur4 283 8 chain chain NN cord-351964-hduv0ur4 283 9 added add VBD cord-351964-hduv0ur4 283 10 to to IN cord-351964-hduv0ur4 283 11 E1 E1 NNP cord-351964-hduv0ur4 283 12 has have VBZ cord-351964-hduv0ur4 283 13 the the DT cord-351964-hduv0ur4 283 14 composition composition NN cord-351964-hduv0ur4 283 15 N n CD cord-351964-hduv0ur4 283 16 - - HYPH cord-351964-hduv0ur4 283 17 acetylgalactosamine acetylgalactosamine JJ cord-351964-hduv0ur4 283 18 - - HYPH cord-351964-hduv0ur4 283 19 galactose galactose NN cord-351964-hduv0ur4 283 20 - - HYPH cord-351964-hduv0ur4 283 21 sialic sialic NN cord-351964-hduv0ur4 283 22 acid acid NN cord-351964-hduv0ur4 283 23 and and CC cord-351964-hduv0ur4 283 24 in in IN cord-351964-hduv0ur4 283 25 ~o65 ~o65 CD cord-351964-hduv0ur4 283 26 % % NN cord-351964-hduv0ur4 283 27 of of IN cord-351964-hduv0ur4 283 28 these these DT cord-351964-hduv0ur4 283 29 chains chain NNS cord-351964-hduv0ur4 284 1 an an DT cord-351964-hduv0ur4 284 2 additional additional JJ cord-351964-hduv0ur4 284 3 sialic sialic NN cord-351964-hduv0ur4 284 4 acid acid NN cord-351964-hduv0ur4 284 5 residue residue NN cord-351964-hduv0ur4 284 6 is be VBZ cord-351964-hduv0ur4 284 7 added add VBN cord-351964-hduv0ur4 284 8 to to IN cord-351964-hduv0ur4 284 9 the the DT cord-351964-hduv0ur4 284 10 N n CD cord-351964-hduv0ur4 284 11 - - HYPH cord-351964-hduv0ur4 284 12 acetylgalactosamine acetylgalactosamine JJ cord-351964-hduv0ur4 284 13 residue residue NN cord-351964-hduv0ur4 284 14 ; ; : cord-351964-hduv0ur4 284 15 moreover moreover RB cord-351964-hduv0ur4 284 16 an an DT cord-351964-hduv0ur4 284 17 average average JJ cord-351964-hduv0ur4 284 18 three three CD cord-351964-hduv0ur4 284 19 of of IN cord-351964-hduv0ur4 284 20 the the DT cord-351964-hduv0ur4 284 21 four four CD cord-351964-hduv0ur4 284 22 amino amino NN cord-351964-hduv0ur4 284 23 - - HYPH cord-351964-hduv0ur4 284 24 terminal terminal NN cord-351964-hduv0ur4 284 25 acceptor acceptor NN cord-351964-hduv0ur4 284 26 amino amino NN cord-351964-hduv0ur4 284 27 acids acid NNS cord-351964-hduv0ur4 284 28 ( ( -LRB- cord-351964-hduv0ur4 284 29 NH2-Ser NH2-Ser NNP cord-351964-hduv0ur4 284 30 - - HYPH cord-351964-hduv0ur4 284 31 Ser Ser NNP cord-351964-hduv0ur4 284 32 - - HYPH cord-351964-hduv0ur4 284 33 Thr Thr NNP cord-351964-hduv0ur4 284 34 - - HYPH cord-351964-hduv0ur4 284 35 Thr Thr NNP cord-351964-hduv0ur4 284 36 ) ) -RRB- cord-351964-hduv0ur4 284 37 are be VBP cord-351964-hduv0ur4 284 38 glycosylated glycosylate VBN cord-351964-hduv0ur4 284 39 ( ( -LRB- cord-351964-hduv0ur4 285 1 Niemann Niemann NNP cord-351964-hduv0ur4 285 2 et et NNP cord-351964-hduv0ur4 285 3 al al NNP cord-351964-hduv0ur4 285 4 . . NNP cord-351964-hduv0ur4 285 5 , , , cord-351964-hduv0ur4 285 6 1984 1984 CD cord-351964-hduv0ur4 285 7 ) ) -RRB- cord-351964-hduv0ur4 285 8 . . . cord-351964-hduv0ur4 286 1 Our -PRON- PRP$ cord-351964-hduv0ur4 286 2 data datum NNS cord-351964-hduv0ur4 286 3 indicate indicate VBP cord-351964-hduv0ur4 286 4 that that IN cord-351964-hduv0ur4 286 5 in in IN cord-351964-hduv0ur4 286 6 AtT20 AtT20 NNP cord-351964-hduv0ur4 286 7 cells cell NNS cord-351964-hduv0ur4 286 8 the the DT cord-351964-hduv0ur4 286 9 O o NN cord-351964-hduv0ur4 286 10 - - HYPH cord-351964-hduv0ur4 286 11 glycosylation glycosylation NN cord-351964-hduv0ur4 286 12 of of IN cord-351964-hduv0ur4 286 13 E1 e1 NN cord-351964-hduv0ur4 286 14 is be VBZ cord-351964-hduv0ur4 286 15 considerably considerably RB cord-351964-hduv0ur4 286 16 simpler simple JJR cord-351964-hduv0ur4 286 17 than than IN cord-351964-hduv0ur4 286 18 in in IN cord-351964-hduv0ur4 286 19 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 286 20 cells cell NNS cord-351964-hduv0ur4 286 21 . . . cord-351964-hduv0ur4 287 1 Different different JJ cord-351964-hduv0ur4 287 2 patterns pattern NNS cord-351964-hduv0ur4 287 3 of of IN cord-351964-hduv0ur4 287 4 O o NN cord-351964-hduv0ur4 287 5 - - HYPH cord-351964-hduv0ur4 287 6 glycosylation glycosylation NN cord-351964-hduv0ur4 287 7 of of IN cord-351964-hduv0ur4 287 8 the the DT cord-351964-hduv0ur4 287 9 same same JJ cord-351964-hduv0ur4 287 10 protein protein NN cord-351964-hduv0ur4 287 11 in in IN cord-351964-hduv0ur4 287 12 different different JJ cord-351964-hduv0ur4 287 13 cell cell NN cord-351964-hduv0ur4 287 14 types type NNS cord-351964-hduv0ur4 287 15 is be VBZ cord-351964-hduv0ur4 287 16 , , , cord-351964-hduv0ur4 287 17 however however RB cord-351964-hduv0ur4 287 18 , , , cord-351964-hduv0ur4 287 19 not not RB cord-351964-hduv0ur4 287 20 unprecedented unprecedented JJ cord-351964-hduv0ur4 287 21 . . . cord-351964-hduv0ur4 288 1 Cummings Cummings NNP cord-351964-hduv0ur4 288 2 et et FW cord-351964-hduv0ur4 288 3 al al NNP cord-351964-hduv0ur4 288 4 . . . cord-351964-hduv0ur4 289 1 ( ( -LRB- cord-351964-hduv0ur4 289 2 1983 1983 CD cord-351964-hduv0ur4 289 3 ) ) -RRB- cord-351964-hduv0ur4 289 4 have have VBP cord-351964-hduv0ur4 289 5 shown show VBN cord-351964-hduv0ur4 289 6 that that IN cord-351964-hduv0ur4 289 7 the the DT cord-351964-hduv0ur4 289 8 low low JJ cord-351964-hduv0ur4 289 9 density density NN cord-351964-hduv0ur4 289 10 lipoprotein lipoprotein NN cord-351964-hduv0ur4 289 11 receptor receptor NN cord-351964-hduv0ur4 289 12 is be VBZ cord-351964-hduv0ur4 289 13 differently differently RB cord-351964-hduv0ur4 289 14 O o NN cord-351964-hduv0ur4 289 15 - - HYPH cord-351964-hduv0ur4 289 16 glycosylated glycosylate VBN cord-351964-hduv0ur4 289 17 in in IN cord-351964-hduv0ur4 289 18 different different JJ cord-351964-hduv0ur4 289 19 cells cell NNS cord-351964-hduv0ur4 289 20 . . . cord-351964-hduv0ur4 290 1 We -PRON- PRP cord-351964-hduv0ur4 290 2 have have VBP cord-351964-hduv0ur4 290 3 now now RB cord-351964-hduv0ur4 290 4 shown show VBN cord-351964-hduv0ur4 290 5 the the DT cord-351964-hduv0ur4 290 6 same same JJ cord-351964-hduv0ur4 290 7 to to TO cord-351964-hduv0ur4 290 8 be be VB cord-351964-hduv0ur4 290 9 true true JJ cord-351964-hduv0ur4 290 10 of of IN cord-351964-hduv0ur4 290 11 the the DT cord-351964-hduv0ur4 290 12 E1 e1 NN cord-351964-hduv0ur4 290 13 glycoprotein glycoprotein NN cord-351964-hduv0ur4 290 14 of of IN cord-351964-hduv0ur4 290 15 MHV MHV NNP cord-351964-hduv0ur4 290 16 - - HYPH cord-351964-hduv0ur4 290 17 A59 A59 NNP cord-351964-hduv0ur4 290 18 . . . cord-351964-hduv0ur4 291 1 In in IN cord-351964-hduv0ur4 291 2 AtT20 AtT20 NNP cord-351964-hduv0ur4 291 3 cells cell NNS cord-351964-hduv0ur4 291 4 , , , cord-351964-hduv0ur4 291 5 at at IN cord-351964-hduv0ur4 291 6 7 7 CD cord-351964-hduv0ur4 291 7 - - SYM cord-351964-hduv0ur4 291 8 10-h 10-h CD cord-351964-hduv0ur4 291 9 postinfection postinfection NN cord-351964-hduv0ur4 291 10 with with IN cord-351964-hduv0ur4 291 11 MHV MHV NNP cord-351964-hduv0ur4 291 12 - - HYPH cord-351964-hduv0ur4 291 13 A59 A59 NNP cord-351964-hduv0ur4 291 14 , , , cord-351964-hduv0ur4 291 15 both both DT cord-351964-hduv0ur4 291 16 progeny progeny JJ cord-351964-hduv0ur4 291 17 virions virion NNS cord-351964-hduv0ur4 291 18 and and CC cord-351964-hduv0ur4 291 19 condensing condense VBG cord-351964-hduv0ur4 291 20 secretory secretory JJ cord-351964-hduv0ur4 291 21 proteins protein NNS cord-351964-hduv0ur4 291 22 accumulate accumulate VBP cord-351964-hduv0ur4 291 23 in in IN cord-351964-hduv0ur4 291 24 the the DT cord-351964-hduv0ur4 291 25 trans trans NNP cord-351964-hduv0ur4 291 26 - - NNP cord-351964-hduv0ur4 291 27 Golgi Golgi NNP cord-351964-hduv0ur4 291 28 network network NN cord-351964-hduv0ur4 291 29 . . . cord-351964-hduv0ur4 292 1 Within within IN cord-351964-hduv0ur4 292 2 that that DT cord-351964-hduv0ur4 292 3 compartment compartment NN cord-351964-hduv0ur4 292 4 condensed condense VBN cord-351964-hduv0ur4 292 5 secretory secretory JJ cord-351964-hduv0ur4 292 6 proteins protein NNS cord-351964-hduv0ur4 292 7 , , , cord-351964-hduv0ur4 292 8 perhaps perhaps RB cord-351964-hduv0ur4 292 9 by by IN cord-351964-hduv0ur4 292 10 a a DT cord-351964-hduv0ur4 292 11 phase phase NN cord-351964-hduv0ur4 292 12 separation separation NN cord-351964-hduv0ur4 292 13 process process NN cord-351964-hduv0ur4 292 14 , , , cord-351964-hduv0ur4 292 15 form form VB cord-351964-hduv0ur4 292 16 the the DT cord-351964-hduv0ur4 292 17 secretory secretory JJ cord-351964-hduv0ur4 292 18 granule granule NN cord-351964-hduv0ur4 292 19 cores core NNS cord-351964-hduv0ur4 292 20 that that WDT cord-351964-hduv0ur4 292 21 exclude exclude VBP cord-351964-hduv0ur4 292 22 virions virion NNS cord-351964-hduv0ur4 292 23 . . . cord-351964-hduv0ur4 293 1 On on IN cord-351964-hduv0ur4 293 2 the the DT cord-351964-hduv0ur4 293 3 other other JJ cord-351964-hduv0ur4 293 4 hand hand NN cord-351964-hduv0ur4 293 5 , , , cord-351964-hduv0ur4 293 6 the the DT cord-351964-hduv0ur4 293 7 post post JJ cord-351964-hduv0ur4 293 8 - - JJ cord-351964-hduv0ur4 293 9 Golgi golgi JJ cord-351964-hduv0ur4 293 10 vesicles vesicle NNS cord-351964-hduv0ur4 293 11 transporting transport VBG cord-351964-hduv0ur4 293 12 mature mature JJ cord-351964-hduv0ur4 293 13 virions virion NNS cord-351964-hduv0ur4 293 14 were be VBD cord-351964-hduv0ur4 293 15 either either CC cord-351964-hduv0ur4 293 16 not not RB cord-351964-hduv0ur4 293 17 labeled label VBN cord-351964-hduv0ur4 293 18 or or CC cord-351964-hduv0ur4 293 19 only only RB cord-351964-hduv0ur4 293 20 very very RB cord-351964-hduv0ur4 293 21 weakly weakly RB cord-351964-hduv0ur4 293 22 labeled label VBN cord-351964-hduv0ur4 293 23 by by IN cord-351964-hduv0ur4 293 24 the the DT cord-351964-hduv0ur4 293 25 immunoperoxidase immunoperoxidase NN cord-351964-hduv0ur4 293 26 reaction reaction NN cord-351964-hduv0ur4 293 27 using use VBG cord-351964-hduv0ur4 293 28 antibody antibody NN cord-351964-hduv0ur4 293 29 that that WDT cord-351964-hduv0ur4 293 30 recognizes recognize VBZ cord-351964-hduv0ur4 293 31 both both CC cord-351964-hduv0ur4 293 32 ACTH ACTH NNP cord-351964-hduv0ur4 293 33 and and CC cord-351964-hduv0ur4 293 34 its -PRON- PRP$ cord-351964-hduv0ur4 293 35 precursor precursor NN cord-351964-hduv0ur4 293 36 , , , cord-351964-hduv0ur4 293 37 proopiomelanocortin proopiomelanocortin NNS cord-351964-hduv0ur4 293 38 . . . cord-351964-hduv0ur4 294 1 Whether whether IN cord-351964-hduv0ur4 294 2 or or CC cord-351964-hduv0ur4 294 3 not not RB cord-351964-hduv0ur4 294 4 there there EX cord-351964-hduv0ur4 294 5 exists exist VBZ cord-351964-hduv0ur4 294 6 in in IN cord-351964-hduv0ur4 294 7 infected infected JJ cord-351964-hduv0ur4 294 8 cells cell NNS cord-351964-hduv0ur4 294 9 a a DT cord-351964-hduv0ur4 294 10 further further JJ cord-351964-hduv0ur4 294 11 class class NN cord-351964-hduv0ur4 294 12 of of IN cord-351964-hduv0ur4 294 13 vesicles vesicle NNS cord-351964-hduv0ur4 294 14 that that WDT cord-351964-hduv0ur4 294 15 constitutively constitutively RB cord-351964-hduv0ur4 294 16 transport transport NN cord-351964-hduv0ur4 294 17 uncleaved uncleave VBN cord-351964-hduv0ur4 294 18 proopiomelanocortin proopiomelanocortin NNS cord-351964-hduv0ur4 294 19 but but CC cord-351964-hduv0ur4 294 20 not not RB cord-351964-hduv0ur4 294 21 virions virion NNS cord-351964-hduv0ur4 294 22 is be VBZ cord-351964-hduv0ur4 294 23 an an DT cord-351964-hduv0ur4 294 24 open open JJ cord-351964-hduv0ur4 294 25 question question NN cord-351964-hduv0ur4 294 26 . . . cord-351964-hduv0ur4 295 1 Furthermore furthermore RB cord-351964-hduv0ur4 295 2 chloroquine chloroquine NNP cord-351964-hduv0ur4 295 3 , , , cord-351964-hduv0ur4 295 4 at at IN cord-351964-hduv0ur4 295 5 200 200 CD cord-351964-hduv0ur4 295 6 lxM lxM NNP cord-351964-hduv0ur4 295 7 , , , cord-351964-hduv0ur4 295 8 does do VBZ cord-351964-hduv0ur4 295 9 not not RB cord-351964-hduv0ur4 295 10 block block VB cord-351964-hduv0ur4 295 11 the the DT cord-351964-hduv0ur4 295 12 formation formation NN cord-351964-hduv0ur4 295 13 of of IN cord-351964-hduv0ur4 295 14 the the DT cord-351964-hduv0ur4 295 15 vesicles vesicle NNS cord-351964-hduv0ur4 295 16 that that WDT cord-351964-hduv0ur4 295 17 transport transport VBP cord-351964-hduv0ur4 295 18 virions virion NNS cord-351964-hduv0ur4 295 19 to to IN cord-351964-hduv0ur4 295 20 the the DT cord-351964-hduv0ur4 295 21 cell cell NN cord-351964-hduv0ur4 295 22 surface surface NN cord-351964-hduv0ur4 295 23 . . . cord-351964-hduv0ur4 296 1 These these DT cord-351964-hduv0ur4 296 2 properties property NNS cord-351964-hduv0ur4 296 3 are be VBP cord-351964-hduv0ur4 296 4 consistent consistent JJ cord-351964-hduv0ur4 296 5 with with IN cord-351964-hduv0ur4 296 6 the the DT cord-351964-hduv0ur4 296 7 interpretation interpretation NN cord-351964-hduv0ur4 296 8 that that IN cord-351964-hduv0ur4 296 9 post post JJ cord-351964-hduv0ur4 296 10 - - JJ cord-351964-hduv0ur4 296 11 Golgi golgi JJ cord-351964-hduv0ur4 296 12 vacuoles vacuole NNS cord-351964-hduv0ur4 296 13 transporting transport VBG cord-351964-hduv0ur4 296 14 progeny progeny JJ cord-351964-hduv0ur4 296 15 MHV mhv NN cord-351964-hduv0ur4 296 16 - - HYPH cord-351964-hduv0ur4 296 17 A59 A59 NNP cord-351964-hduv0ur4 296 18 are be VBP cord-351964-hduv0ur4 296 19 part part NN cord-351964-hduv0ur4 296 20 of of IN cord-351964-hduv0ur4 296 21 the the DT cord-351964-hduv0ur4 296 22 constitutive constitutive JJ cord-351964-hduv0ur4 296 23 exocytic exocytic JJ cord-351964-hduv0ur4 296 24 pathway pathway NN cord-351964-hduv0ur4 296 25 . . . cord-351964-hduv0ur4 297 1 Using use VBG cord-351964-hduv0ur4 297 2 the the DT cord-351964-hduv0ur4 297 3 presence presence NN cord-351964-hduv0ur4 297 4 of of IN cord-351964-hduv0ur4 297 5 virions virion NNS cord-351964-hduv0ur4 297 6 as as IN cord-351964-hduv0ur4 297 7 a a DT cord-351964-hduv0ur4 297 8 morphological morphological JJ cord-351964-hduv0ur4 297 9 marker marker NN cord-351964-hduv0ur4 297 10 of of IN cord-351964-hduv0ur4 297 11 the the DT cord-351964-hduv0ur4 297 12 constitutive constitutive JJ cord-351964-hduv0ur4 297 13 pathway pathway NN cord-351964-hduv0ur4 297 14 in in IN cord-351964-hduv0ur4 297 15 infected infected JJ cord-351964-hduv0ur4 297 16 AtT20 AtT20 NNP cord-351964-hduv0ur4 297 17 cells cell NNS cord-351964-hduv0ur4 297 18 , , , cord-351964-hduv0ur4 297 19 we -PRON- PRP cord-351964-hduv0ur4 297 20 can can MD cord-351964-hduv0ur4 297 21 conclude conclude VB cord-351964-hduv0ur4 297 22 that that IN cord-351964-hduv0ur4 297 23 the the DT cord-351964-hduv0ur4 297 24 trans trans NNP cord-351964-hduv0ur4 297 25 - - NNP cord-351964-hduv0ur4 297 26 Golgi Golgi NNP cord-351964-hduv0ur4 297 27 network network NN cord-351964-hduv0ur4 297 28 is be VBZ cord-351964-hduv0ur4 297 29 the the DT cord-351964-hduv0ur4 297 30 compartment compartment NN cord-351964-hduv0ur4 297 31 from from IN cord-351964-hduv0ur4 297 32 which which WDT cord-351964-hduv0ur4 297 33 the the DT cord-351964-hduv0ur4 297 34 constitutive constitutive JJ cord-351964-hduv0ur4 297 35 and and CC cord-351964-hduv0ur4 297 36 regulated regulated JJ cord-351964-hduv0ur4 297 37 pathways pathway NNS cord-351964-hduv0ur4 297 38 diverge diverge VBP cord-351964-hduv0ur4 297 39 in in IN cord-351964-hduv0ur4 297 40 these these DT cord-351964-hduv0ur4 297 41 cells cell NNS cord-351964-hduv0ur4 297 42 . . . cord-351964-hduv0ur4 298 1 The the DT cord-351964-hduv0ur4 298 2 fact fact NN cord-351964-hduv0ur4 298 3 that that IN cord-351964-hduv0ur4 298 4 extremely extremely RB cord-351964-hduv0ur4 298 5 rare rare JJ cord-351964-hduv0ur4 298 6 post post JJ cord-351964-hduv0ur4 298 7 - - JJ cord-351964-hduv0ur4 298 8 Golgi golgi JJ cord-351964-hduv0ur4 298 9 vesicles vesicle NNS cord-351964-hduv0ur4 298 10 located locate VBN cord-351964-hduv0ur4 298 11 at at IN cord-351964-hduv0ur4 298 12 the the DT cord-351964-hduv0ur4 298 13 cell cell NN cord-351964-hduv0ur4 298 14 periphery periphery NN cord-351964-hduv0ur4 298 15 and and CC cord-351964-hduv0ur4 298 16 packed pack VBN cord-351964-hduv0ur4 298 17 with with IN cord-351964-hduv0ur4 298 18 virions virion NNS cord-351964-hduv0ur4 298 19 also also RB cord-351964-hduv0ur4 298 20 contain contain VBP cord-351964-hduv0ur4 298 21 clumps clump NNS cord-351964-hduv0ur4 298 22 of of IN cord-351964-hduv0ur4 298 23 condensed condense VBN cord-351964-hduv0ur4 298 24 secretory secretory JJ cord-351964-hduv0ur4 298 25 protein protein NN cord-351964-hduv0ur4 298 26 indicates indicate VBZ cord-351964-hduv0ur4 298 27 that that IN cord-351964-hduv0ur4 298 28 although although IN cord-351964-hduv0ur4 298 29 sorting sort VBG cord-351964-hduv0ur4 298 30 of of IN cord-351964-hduv0ur4 298 31 condensed condense VBN cord-351964-hduv0ur4 298 32 secretory secretory JJ cord-351964-hduv0ur4 298 33 proteins protein NNS cord-351964-hduv0ur4 298 34 into into IN cord-351964-hduv0ur4 298 35 the the DT cord-351964-hduv0ur4 298 36 cores core NNS cord-351964-hduv0ur4 298 37 of of IN cord-351964-hduv0ur4 298 38 secretory secretory JJ cord-351964-hduv0ur4 298 39 granules granule NNS cord-351964-hduv0ur4 298 40 of of IN cord-351964-hduv0ur4 298 41 the the DT cord-351964-hduv0ur4 298 42 regulated regulated JJ cord-351964-hduv0ur4 298 43 pathway pathway NN cord-351964-hduv0ur4 298 44 is be VBZ cord-351964-hduv0ur4 298 45 efficient efficient JJ cord-351964-hduv0ur4 298 46 , , , cord-351964-hduv0ur4 298 47 it -PRON- PRP cord-351964-hduv0ur4 298 48 is be VBZ cord-351964-hduv0ur4 298 49 not not RB cord-351964-hduv0ur4 298 50 absolutely absolutely RB cord-351964-hduv0ur4 298 51 rigorous rigorous JJ cord-351964-hduv0ur4 298 52 . . . cord-351964-hduv0ur4 299 1 A a DT cord-351964-hduv0ur4 299 2 number number NN cord-351964-hduv0ur4 299 3 of of IN cord-351964-hduv0ur4 299 4 groups group NNS cord-351964-hduv0ur4 299 5 have have VBP cord-351964-hduv0ur4 299 6 invested invest VBN cord-351964-hduv0ur4 299 7 a a DT cord-351964-hduv0ur4 299 8 great great JJ cord-351964-hduv0ur4 299 9 deal deal NN cord-351964-hduv0ur4 299 10 of of IN cord-351964-hduv0ur4 299 11 effort effort NN cord-351964-hduv0ur4 299 12 in in IN cord-351964-hduv0ur4 299 13 attempting attempt VBG cord-351964-hduv0ur4 299 14 to to TO cord-351964-hduv0ur4 299 15 identify identify VB cord-351964-hduv0ur4 299 16 the the DT cord-351964-hduv0ur4 299 17 site site NN cord-351964-hduv0ur4 299 18 of of IN cord-351964-hduv0ur4 299 19 divergence divergence NN cord-351964-hduv0ur4 299 20 of of IN cord-351964-hduv0ur4 299 21 intracellular intracellular JJ cord-351964-hduv0ur4 299 22 transport transport NN cord-351964-hduv0ur4 299 23 pathways pathway NNS cord-351964-hduv0ur4 299 24 to to IN cord-351964-hduv0ur4 299 25 the the DT cord-351964-hduv0ur4 299 26 cell cell NN cord-351964-hduv0ur4 299 27 surface surface NN cord-351964-hduv0ur4 299 28 . . . cord-351964-hduv0ur4 300 1 This this DT cord-351964-hduv0ur4 300 2 compartment compartment NN cord-351964-hduv0ur4 300 3 will will MD cord-351964-hduv0ur4 300 4 be be VB cord-351964-hduv0ur4 300 5 the the DT cord-351964-hduv0ur4 300 6 major major JJ cord-351964-hduv0ur4 300 7 site site NN cord-351964-hduv0ur4 300 8 for for IN cord-351964-hduv0ur4 300 9 the the DT cord-351964-hduv0ur4 300 10 sorting sorting NN cord-351964-hduv0ur4 300 11 of of IN cord-351964-hduv0ur4 300 12 newly newly RB cord-351964-hduv0ur4 300 13 synthesized synthesize VBN cord-351964-hduv0ur4 300 14 plasma plasma NN cord-351964-hduv0ur4 300 15 membrane membrane NN cord-351964-hduv0ur4 300 16 and and CC cord-351964-hduv0ur4 300 17 secretory secretory NN cord-351964-hduv0ur4 300 18 proteins protein NNS cord-351964-hduv0ur4 300 19 . . . cord-351964-hduv0ur4 301 1 The the DT cord-351964-hduv0ur4 301 2 evidence evidence NN cord-351964-hduv0ur4 301 3 accumulated accumulate VBN cord-351964-hduv0ur4 301 4 so so RB cord-351964-hduv0ur4 301 5 far far RB cord-351964-hduv0ur4 301 6 has have VBZ cord-351964-hduv0ur4 301 7 narrowed narrow VBN cord-351964-hduv0ur4 301 8 down down RP cord-351964-hduv0ur4 301 9 but but CC cord-351964-hduv0ur4 301 10 not not RB cord-351964-hduv0ur4 301 11 identified identify VBD cord-351964-hduv0ur4 301 12 the the DT cord-351964-hduv0ur4 301 13 site site NN cord-351964-hduv0ur4 301 14 of of IN cord-351964-hduv0ur4 301 15 sorting sorting NN cord-351964-hduv0ur4 301 16 either either CC cord-351964-hduv0ur4 301 17 for for IN cord-351964-hduv0ur4 301 18 components component NNS cord-351964-hduv0ur4 301 19 destined destine VBN cord-351964-hduv0ur4 301 20 for for IN cord-351964-hduv0ur4 301 21 the the DT cord-351964-hduv0ur4 301 22 constitutive constitutive JJ cord-351964-hduv0ur4 301 23 and and CC cord-351964-hduv0ur4 301 24 regulated regulated JJ cord-351964-hduv0ur4 301 25 pathways pathway NNS cord-351964-hduv0ur4 301 26 of of IN cord-351964-hduv0ur4 301 27 secretion secretion NN cord-351964-hduv0ur4 301 28 or or CC cord-351964-hduv0ur4 301 29 for for IN cord-351964-hduv0ur4 301 30 membrane membrane NN cord-351964-hduv0ur4 301 31 proteins protein NNS cord-351964-hduv0ur4 301 32 destined destine VBN cord-351964-hduv0ur4 301 33 for for IN cord-351964-hduv0ur4 301 34 different different JJ cord-351964-hduv0ur4 301 35 plasma plasma NN cord-351964-hduv0ur4 301 36 membrane membrane NN cord-351964-hduv0ur4 301 37 domains domain NNS cord-351964-hduv0ur4 301 38 ( ( -LRB- cord-351964-hduv0ur4 301 39 reviewed review VBN cord-351964-hduv0ur4 301 40 in in IN cord-351964-hduv0ur4 301 41 Griffiths Griffiths NNP cord-351964-hduv0ur4 301 42 and and CC cord-351964-hduv0ur4 301 43 Simons Simons NNP cord-351964-hduv0ur4 301 44 , , , cord-351964-hduv0ur4 301 45 1986 1986 CD cord-351964-hduv0ur4 301 46 ) ) -RRB- cord-351964-hduv0ur4 301 47 . . . cord-351964-hduv0ur4 302 1 Biochemical biochemical JJ cord-351964-hduv0ur4 302 2 and and CC cord-351964-hduv0ur4 302 3 morphological morphological JJ cord-351964-hduv0ur4 302 4 studies study NNS cord-351964-hduv0ur4 302 5 ( ( -LRB- cord-351964-hduv0ur4 302 6 Rindler Rindler NNP cord-351964-hduv0ur4 302 7 et et NNP cord-351964-hduv0ur4 302 8 al al NNP cord-351964-hduv0ur4 302 9 . . NNP cord-351964-hduv0ur4 302 10 , , , cord-351964-hduv0ur4 302 11 1984 1984 CD cord-351964-hduv0ur4 302 12 ) ) -RRB- cord-351964-hduv0ur4 302 13 indicate indicate VBP cord-351964-hduv0ur4 302 14 that that IN cord-351964-hduv0ur4 302 15 proteins protein NNS cord-351964-hduv0ur4 302 16 destined destine VBN cord-351964-hduv0ur4 302 17 for for IN cord-351964-hduv0ur4 302 18 separate separate JJ cord-351964-hduv0ur4 302 19 cell cell NN cord-351964-hduv0ur4 302 20 surfaces surface NNS cord-351964-hduv0ur4 302 21 colocalize colocalize NN cord-351964-hduv0ur4 302 22 throughout throughout IN cord-351964-hduv0ur4 302 23 the the DT cord-351964-hduv0ur4 302 24 Golgi Golgi NNP cord-351964-hduv0ur4 302 25 stack stack NN cord-351964-hduv0ur4 302 26 and and CC cord-351964-hduv0ur4 302 27 that that IN cord-351964-hduv0ur4 302 28 they -PRON- PRP cord-351964-hduv0ur4 302 29 separate separate VBP cord-351964-hduv0ur4 302 30 before before IN cord-351964-hduv0ur4 302 31 reaching reach VBG cord-351964-hduv0ur4 302 32 the the DT cord-351964-hduv0ur4 302 33 plasma plasma NN cord-351964-hduv0ur4 302 34 membrane membrane NN cord-351964-hduv0ur4 302 35 ( ( -LRB- cord-351964-hduv0ur4 302 36 Kelly Kelly NNP cord-351964-hduv0ur4 302 37 , , , cord-351964-hduv0ur4 302 38 1985 1985 CD cord-351964-hduv0ur4 302 39 ; ; : cord-351964-hduv0ur4 302 40 Marlin Marlin NNP cord-351964-hduv0ur4 302 41 and and CC cord-351964-hduv0ur4 302 42 Simons Simons NNP cord-351964-hduv0ur4 302 43 , , , cord-351964-hduv0ur4 302 44 1984 1984 CD cord-351964-hduv0ur4 302 45 ; ; : cord-351964-hduv0ur4 302 46 Misek Misek NNP cord-351964-hduv0ur4 302 47 et et NNP cord-351964-hduv0ur4 302 48 al al NNP cord-351964-hduv0ur4 302 49 . . NNP cord-351964-hduv0ur4 302 50 , , , cord-351964-hduv0ur4 302 51 1984 1984 CD cord-351964-hduv0ur4 302 52 ; ; : cord-351964-hduv0ur4 302 53 Pfeiffer Pfeiffer NNP cord-351964-hduv0ur4 302 54 et et NNP cord-351964-hduv0ur4 302 55 al al NNP cord-351964-hduv0ur4 302 56 . . NNP cord-351964-hduv0ur4 302 57 , , , cord-351964-hduv0ur4 302 58 1985 1985 CD cord-351964-hduv0ur4 302 59 ) ) -RRB- cord-351964-hduv0ur4 302 60 . . . cord-351964-hduv0ur4 303 1 Griffiths Griffiths NNP cord-351964-hduv0ur4 303 2 and and CC cord-351964-hduv0ur4 303 3 Simons Simons NNP cord-351964-hduv0ur4 303 4 ( ( -LRB- cord-351964-hduv0ur4 303 5 1986 1986 CD cord-351964-hduv0ur4 303 6 ) ) -RRB- cord-351964-hduv0ur4 303 7 proposed propose VBD cord-351964-hduv0ur4 303 8 that that IN cord-351964-hduv0ur4 303 9 the the DT cord-351964-hduv0ur4 303 10 sorting sorting NN cord-351964-hduv0ur4 303 11 of of IN cord-351964-hduv0ur4 303 12 these these DT cord-351964-hduv0ur4 303 13 components component NNS cord-351964-hduv0ur4 303 14 occurs occur VBZ cord-351964-hduv0ur4 303 15 in in IN cord-351964-hduv0ur4 303 16 the the DT cord-351964-hduv0ur4 303 17 trans trans NNP cord-351964-hduv0ur4 303 18 - - NNP cord-351964-hduv0ur4 303 19 Golgi Golgi NNP cord-351964-hduv0ur4 303 20 network network NN cord-351964-hduv0ur4 303 21 since since IN cord-351964-hduv0ur4 303 22 components component NNS cord-351964-hduv0ur4 303 23 to to TO cord-351964-hduv0ur4 303 24 be be VB cord-351964-hduv0ur4 303 25 sorted sort VBN cord-351964-hduv0ur4 303 26 are be VBP cord-351964-hduv0ur4 303 27 in in IN cord-351964-hduv0ur4 303 28 contact contact NN cord-351964-hduv0ur4 303 29 during during IN cord-351964-hduv0ur4 303 30 the the DT cord-351964-hduv0ur4 303 31 last last JJ cord-351964-hduv0ur4 303 32 identifable identifable JJ cord-351964-hduv0ur4 303 33 steps step NNS cord-351964-hduv0ur4 303 34 of of IN cord-351964-hduv0ur4 303 35 Golgi Golgi NNP cord-351964-hduv0ur4 303 36 processing processing NN cord-351964-hduv0ur4 303 37 . . . cord-351964-hduv0ur4 304 1 A a DT cord-351964-hduv0ur4 304 2 critical critical JJ cord-351964-hduv0ur4 304 3 test test NN cord-351964-hduv0ur4 304 4 of of IN cord-351964-hduv0ur4 304 5 this this DT cord-351964-hduv0ur4 304 6 hypothesis hypothesis NN cord-351964-hduv0ur4 304 7 has have VBZ cord-351964-hduv0ur4 304 8 been be VBN cord-351964-hduv0ur4 304 9 difficult difficult JJ cord-351964-hduv0ur4 304 10 since since IN cord-351964-hduv0ur4 304 11 it -PRON- PRP cord-351964-hduv0ur4 304 12 requires require VBZ cord-351964-hduv0ur4 304 13 simultaneous simultaneous JJ cord-351964-hduv0ur4 304 14 observation observation NN cord-351964-hduv0ur4 304 15 of of IN cord-351964-hduv0ur4 304 16 components component NNS cord-351964-hduv0ur4 304 17 destined destine VBN cord-351964-hduv0ur4 304 18 for for IN cord-351964-hduv0ur4 304 19 the the DT cord-351964-hduv0ur4 304 20 two two CD cord-351964-hduv0ur4 304 21 pathways pathway NNS cord-351964-hduv0ur4 304 22 . . . cord-351964-hduv0ur4 305 1 The the DT cord-351964-hduv0ur4 305 2 experiment experiment NN cord-351964-hduv0ur4 305 3 must must MD cord-351964-hduv0ur4 305 4 also also RB cord-351964-hduv0ur4 305 5 be be VB cord-351964-hduv0ur4 305 6 done do VBN cord-351964-hduv0ur4 305 7 with with IN cord-351964-hduv0ur4 305 8 sufficient sufficient JJ cord-351964-hduv0ur4 305 9 time time NN cord-351964-hduv0ur4 305 10 resolution resolution NN cord-351964-hduv0ur4 305 11 to to TO cord-351964-hduv0ur4 305 12 rule rule VB cord-351964-hduv0ur4 305 13 out out RP cord-351964-hduv0ur4 305 14 the the DT cord-351964-hduv0ur4 305 15 possibility possibility NN cord-351964-hduv0ur4 305 16 of of IN cord-351964-hduv0ur4 305 17 transient transient JJ cord-351964-hduv0ur4 305 18 formation formation NN cord-351964-hduv0ur4 305 19 of of IN cord-351964-hduv0ur4 305 20 a a DT cord-351964-hduv0ur4 305 21 compartment compartment NN cord-351964-hduv0ur4 305 22 beyond beyond IN cord-351964-hduv0ur4 305 23 the the DT cord-351964-hduv0ur4 305 24 trans trans NNP cord-351964-hduv0ur4 305 25 - - NNP cord-351964-hduv0ur4 305 26 Golgi Golgi NNP cord-351964-hduv0ur4 305 27 network network NN cord-351964-hduv0ur4 305 28 which which WDT cord-351964-hduv0ur4 305 29 fissions fission VBZ cord-351964-hduv0ur4 305 30 to to TO cord-351964-hduv0ur4 305 31 produce produce VB cord-351964-hduv0ur4 305 32 sorted sort VBN cord-351964-hduv0ur4 305 33 transport transport NN cord-351964-hduv0ur4 305 34 vesicles vesicle NNS cord-351964-hduv0ur4 305 35 . . . cord-351964-hduv0ur4 306 1 The the DT cord-351964-hduv0ur4 306 2 system system NN cord-351964-hduv0ur4 306 3 we -PRON- PRP cord-351964-hduv0ur4 306 4 use use VBP cord-351964-hduv0ur4 306 5 here here RB cord-351964-hduv0ur4 306 6 fulfills fulfill VBZ cord-351964-hduv0ur4 306 7 these these DT cord-351964-hduv0ur4 306 8 requirements requirement NNS cord-351964-hduv0ur4 306 9 because because IN cord-351964-hduv0ur4 306 10 it -PRON- PRP cord-351964-hduv0ur4 306 11 allows allow VBZ cord-351964-hduv0ur4 306 12 us -PRON- PRP cord-351964-hduv0ur4 306 13 to to TO cord-351964-hduv0ur4 306 14 observe observe VB cord-351964-hduv0ur4 306 15 directly directly RB cord-351964-hduv0ur4 306 16 the the DT cord-351964-hduv0ur4 306 17 separation separation NN cord-351964-hduv0ur4 306 18 of of IN cord-351964-hduv0ur4 306 19 the the DT cord-351964-hduv0ur4 306 20 constitutive constitutive JJ cord-351964-hduv0ur4 306 21 and and CC cord-351964-hduv0ur4 306 22 regulated regulated JJ cord-351964-hduv0ur4 306 23 exocytic exocytic JJ cord-351964-hduv0ur4 306 24 pathways pathway NNS cord-351964-hduv0ur4 306 25 . . . cord-351964-hduv0ur4 307 1 As as RB cord-351964-hduv0ur4 307 2 far far RB cord-351964-hduv0ur4 307 3 as as IN cord-351964-hduv0ur4 307 4 we -PRON- PRP cord-351964-hduv0ur4 307 5 are be VBP cord-351964-hduv0ur4 307 6 aware aware JJ cord-351964-hduv0ur4 307 7 , , , cord-351964-hduv0ur4 307 8 these these DT cord-351964-hduv0ur4 307 9 observations observation NNS cord-351964-hduv0ur4 307 10 are be VBP cord-351964-hduv0ur4 307 11 the the DT cord-351964-hduv0ur4 307 12 first first JJ cord-351964-hduv0ur4 307 13 direct direct JJ cord-351964-hduv0ur4 307 14 visualization visualization NN cord-351964-hduv0ur4 307 15 of of IN cord-351964-hduv0ur4 307 16 the the DT cord-351964-hduv0ur4 307 17 sorting sorting NN cord-351964-hduv0ur4 307 18 at at IN cord-351964-hduv0ur4 307 19 the the DT cord-351964-hduv0ur4 307 20 exit exit NN cord-351964-hduv0ur4 307 21 of of IN cord-351964-hduv0ur4 307 22 the the DT cord-351964-hduv0ur4 307 23 trans trans NNP cord-351964-hduv0ur4 307 24 - - NNP cord-351964-hduv0ur4 307 25 Golgi Golgi NNP cord-351964-hduv0ur4 307 26 network network NN cord-351964-hduv0ur4 307 27 of of IN cord-351964-hduv0ur4 307 28 components component NNS cord-351964-hduv0ur4 307 29 taking take VBG cord-351964-hduv0ur4 307 30 different different JJ cord-351964-hduv0ur4 307 31 routes route NNS cord-351964-hduv0ur4 307 32 to to IN cord-351964-hduv0ur4 307 33 the the DT cord-351964-hduv0ur4 307 34 cell cell NN cord-351964-hduv0ur4 307 35 surface surface NN cord-351964-hduv0ur4 307 36 . . . cord-351964-hduv0ur4 308 1 They -PRON- PRP cord-351964-hduv0ur4 308 2 rely rely VBP cord-351964-hduv0ur4 308 3 on on IN cord-351964-hduv0ur4 308 4 having have VBG cord-351964-hduv0ur4 308 5 readily readily RB cord-351964-hduv0ur4 308 6 distinguishable distinguishable JJ cord-351964-hduv0ur4 308 7 morphological morphological JJ cord-351964-hduv0ur4 308 8 markers marker NNS cord-351964-hduv0ur4 308 9 for for IN cord-351964-hduv0ur4 308 10 each each DT cord-351964-hduv0ur4 308 11 pathway pathway NN cord-351964-hduv0ur4 308 12 , , , cord-351964-hduv0ur4 308 13 and and CC cord-351964-hduv0ur4 308 14 they -PRON- PRP cord-351964-hduv0ur4 308 15 establish establish VBP cord-351964-hduv0ur4 308 16 for for IN cord-351964-hduv0ur4 308 17 this this DT cord-351964-hduv0ur4 308 18 system system NN cord-351964-hduv0ur4 308 19 that that IN cord-351964-hduv0ur4 308 20 there there EX cord-351964-hduv0ur4 308 21 is be VBZ cord-351964-hduv0ur4 308 22 no no DT cord-351964-hduv0ur4 308 23 sorting sorting NN cord-351964-hduv0ur4 308 24 compartment compartment NN cord-351964-hduv0ur4 308 25 beyond beyond IN cord-351964-hduv0ur4 308 26 the the DT cord-351964-hduv0ur4 308 27 trans trans NNP cord-351964-hduv0ur4 308 28 - - NNP cord-351964-hduv0ur4 308 29 Golgi Golgi NNP cord-351964-hduv0ur4 308 30 network network NN cord-351964-hduv0ur4 308 31 . . . cord-351964-hduv0ur4 309 1 In in IN cord-351964-hduv0ur4 309 2 both both DT cord-351964-hduv0ur4 309 3 AtT20 AtT20 NNP cord-351964-hduv0ur4 309 4 cells cell NNS cord-351964-hduv0ur4 309 5 and and CC cord-351964-hduv0ur4 309 6 in in IN cord-351964-hduv0ur4 309 7 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 309 8 host host NN cord-351964-hduv0ur4 309 9 cells cell NNS cord-351964-hduv0ur4 309 10 ( ( -LRB- cord-351964-hduv0ur4 309 11 our -PRON- PRP$ cord-351964-hduv0ur4 309 12 unpublished unpublished JJ cord-351964-hduv0ur4 309 13 observations observation NNS cord-351964-hduv0ur4 309 14 ) ) -RRB- cord-351964-hduv0ur4 309 15 the the DT cord-351964-hduv0ur4 309 16 post post JJ cord-351964-hduv0ur4 309 17 - - JJ cord-351964-hduv0ur4 309 18 Golgi golgi JJ cord-351964-hduv0ur4 309 19 vesicles vesicle NNS cord-351964-hduv0ur4 309 20 that that WDT cord-351964-hduv0ur4 309 21 apparently apparently RB cord-351964-hduv0ur4 309 22 transport transport VBP cord-351964-hduv0ur4 309 23 progeny progeny NN cord-351964-hduv0ur4 309 24 coronavirus coronavirus NN cord-351964-hduv0ur4 309 25 to to IN cord-351964-hduv0ur4 309 26 the the DT cord-351964-hduv0ur4 309 27 cell cell NN cord-351964-hduv0ur4 309 28 surface surface NN cord-351964-hduv0ur4 309 29 are be VBP cord-351964-hduv0ur4 309 30 large large JJ cord-351964-hduv0ur4 309 31 structures structure NNS cord-351964-hduv0ur4 309 32 , , , cord-351964-hduv0ur4 309 33 holding hold VBG cord-351964-hduv0ur4 309 34 several several JJ cord-351964-hduv0ur4 309 35 virions virion NNS cord-351964-hduv0ur4 310 1 each each DT cord-351964-hduv0ur4 310 2 ~1,000 ~1,000 . cord-351964-hduv0ur4 311 1 A a DT cord-351964-hduv0ur4 311 2 in in IN cord-351964-hduv0ur4 311 3 diameter diameter NN cord-351964-hduv0ur4 311 4 . . . cord-351964-hduv0ur4 312 1 These these DT cord-351964-hduv0ur4 312 2 vesicles vesicle NNS cord-351964-hduv0ur4 312 3 occur occur VBP cord-351964-hduv0ur4 312 4 close close RB cord-351964-hduv0ur4 312 5 to to IN cord-351964-hduv0ur4 312 6 the the DT cord-351964-hduv0ur4 312 7 plasma plasma NN cord-351964-hduv0ur4 312 8 membrane membrane NN cord-351964-hduv0ur4 312 9 and and CC cord-351964-hduv0ur4 312 10 lack lack JJ cord-351964-hduv0ur4 312 11 clathrin clathrin NN cord-351964-hduv0ur4 312 12 coats coat NNS cord-351964-hduv0ur4 312 13 on on IN cord-351964-hduv0ur4 312 14 their -PRON- PRP$ cord-351964-hduv0ur4 312 15 cytoplasmic cytoplasmic JJ cord-351964-hduv0ur4 312 16 surface surface NN cord-351964-hduv0ur4 312 17 . . . cord-351964-hduv0ur4 313 1 In in IN cord-351964-hduv0ur4 313 2 uninfected uninfected JJ cord-351964-hduv0ur4 313 3 cells cell NNS cord-351964-hduv0ur4 313 4 there there EX cord-351964-hduv0ur4 313 5 are be VBP cord-351964-hduv0ur4 313 6 no no DT cord-351964-hduv0ur4 313 7 post post JJ cord-351964-hduv0ur4 313 8 - - JJ cord-351964-hduv0ur4 313 9 Golgi golgi JJ cord-351964-hduv0ur4 313 10 vesicles vesicle NNS cord-351964-hduv0ur4 313 11 of of IN cord-351964-hduv0ur4 313 12 this this DT cord-351964-hduv0ur4 313 13 size size NN cord-351964-hduv0ur4 313 14 ; ; . cord-351964-hduv0ur4 314 1 no no RB cord-351964-hduv0ur4 314 2 doubt doubt RB cord-351964-hduv0ur4 314 3 the the DT cord-351964-hduv0ur4 314 4 vesicles vesicle NNS cord-351964-hduv0ur4 314 5 responsible responsible JJ cord-351964-hduv0ur4 314 6 for for IN cord-351964-hduv0ur4 314 7 the the DT cord-351964-hduv0ur4 314 8 constitutive constitutive JJ cord-351964-hduv0ur4 314 9 transport transport NN cord-351964-hduv0ur4 314 10 of of IN cord-351964-hduv0ur4 314 11 endogenous endogenous JJ cord-351964-hduv0ur4 314 12 proteins protein NNS cord-351964-hduv0ur4 314 13 are be VBP cord-351964-hduv0ur4 314 14 much much RB cord-351964-hduv0ur4 314 15 smaller small JJR cord-351964-hduv0ur4 314 16 . . . cord-351964-hduv0ur4 315 1 With with IN cord-351964-hduv0ur4 315 2 the the DT cord-351964-hduv0ur4 315 3 exception exception NN cord-351964-hduv0ur4 315 4 of of IN cord-351964-hduv0ur4 315 5 hepatocytes hepatocyte NNS cord-351964-hduv0ur4 315 6 , , , cord-351964-hduv0ur4 315 7 which which WDT cord-351964-hduv0ur4 315 8 exocytose exocytose NNP cord-351964-hduv0ur4 315 9 v. v. CC cord-351964-hduv0ur4 315 10 low low JJ cord-351964-hduv0ur4 315 11 density density NN cord-351964-hduv0ur4 315 12 lipoprotein lipoprotein NN cord-351964-hduv0ur4 315 13 particles particle NNS cord-351964-hduv0ur4 315 14 , , , cord-351964-hduv0ur4 315 15 mammalian mammalian JJ cord-351964-hduv0ur4 315 16 cells cell NNS cord-351964-hduv0ur4 315 17 normally normally RB cord-351964-hduv0ur4 315 18 never never RB cord-351964-hduv0ur4 315 19 transport transport VBP cord-351964-hduv0ur4 315 20 large large JJ cord-351964-hduv0ur4 315 21 particles particle NNS cord-351964-hduv0ur4 315 22 along along IN cord-351964-hduv0ur4 315 23 the the DT cord-351964-hduv0ur4 315 24 entire entire JJ cord-351964-hduv0ur4 315 25 exocytic exocytic JJ cord-351964-hduv0ur4 315 26 pathway pathway NN cord-351964-hduv0ur4 315 27 but but CC cord-351964-hduv0ur4 315 28 only only RB cord-351964-hduv0ur4 315 29 membrane membrane NN cord-351964-hduv0ur4 315 30 proteins protein NNS cord-351964-hduv0ur4 315 31 and and CC cord-351964-hduv0ur4 315 32 soluble soluble JJ cord-351964-hduv0ur4 315 33 proteins protein NNS cord-351964-hduv0ur4 315 34 . . . cord-351964-hduv0ur4 316 1 However however RB cord-351964-hduv0ur4 316 2 , , , cord-351964-hduv0ur4 316 3 the the DT cord-351964-hduv0ur4 316 4 very very JJ cord-351964-hduv0ur4 316 5 fact fact NN cord-351964-hduv0ur4 316 6 that that IN cord-351964-hduv0ur4 316 7 cells cell NNS cord-351964-hduv0ur4 316 8 of of IN cord-351964-hduv0ur4 316 9 both both DT cord-351964-hduv0ur4 316 10 epithelial epithelial JJ cord-351964-hduv0ur4 316 11 ( ( -LRB- cord-351964-hduv0ur4 316 12 AtT20 AtT20 NNP cord-351964-hduv0ur4 316 13 ) ) -RRB- cord-351964-hduv0ur4 316 14 and and CC cord-351964-hduv0ur4 316 15 fibroblastic fibroblastic JJ cord-351964-hduv0ur4 316 16 ( ( -LRB- cord-351964-hduv0ur4 316 17 sac- sac- NN cord-351964-hduv0ur4 316 18 , , , cord-351964-hduv0ur4 316 19 17Cll 17cll CD cord-351964-hduv0ur4 316 20 cells cell NNS cord-351964-hduv0ur4 316 21 ) ) -RRB- cord-351964-hduv0ur4 316 22 origin origin NN cord-351964-hduv0ur4 316 23 can can MD cord-351964-hduv0ur4 316 24 accomodate accomodate VB cord-351964-hduv0ur4 316 25 to to IN cord-351964-hduv0ur4 316 26 the the DT cord-351964-hduv0ur4 316 27 transport transport NN cord-351964-hduv0ur4 316 28 of of IN cord-351964-hduv0ur4 316 29 coronavirions coronavirion NNS cord-351964-hduv0ur4 316 30 from from IN cord-351964-hduv0ur4 316 31 pre pre JJ cord-351964-hduv0ur4 316 32 - - JJ cord-351964-hduv0ur4 316 33 Golgi Golgi NNP cord-351964-hduv0ur4 316 34 compartments compartment NNS cord-351964-hduv0ur4 316 35 to to IN cord-351964-hduv0ur4 316 36 the the DT cord-351964-hduv0ur4 316 37 cell cell NN cord-351964-hduv0ur4 316 38 surface surface NN cord-351964-hduv0ur4 316 39 proves prove VBZ cord-351964-hduv0ur4 316 40 that that IN cord-351964-hduv0ur4 316 41 the the DT cord-351964-hduv0ur4 316 42 transport transport NN cord-351964-hduv0ur4 316 43 vesicles vesicle NNS cord-351964-hduv0ur4 316 44 at at IN cord-351964-hduv0ur4 316 45 all all DT cord-351964-hduv0ur4 316 46 stages stage NNS cord-351964-hduv0ur4 316 47 of of IN cord-351964-hduv0ur4 316 48 the the DT cord-351964-hduv0ur4 316 49 exocytic exocytic JJ cord-351964-hduv0ur4 316 50 pathway pathway NN cord-351964-hduv0ur4 316 51 have have VBP cord-351964-hduv0ur4 316 52 the the DT cord-351964-hduv0ur4 316 53 capacity capacity NN cord-351964-hduv0ur4 316 54 to to TO cord-351964-hduv0ur4 316 55 enlarge enlarge VB cord-351964-hduv0ur4 316 56 to to TO cord-351964-hduv0ur4 316 57 carry carry VB cord-351964-hduv0ur4 316 58 large large JJ cord-351964-hduv0ur4 316 59 particulate particulate NN cord-351964-hduv0ur4 316 60 macromolecular macromolecular JJ cord-351964-hduv0ur4 316 61 assemblies assembly NNS cord-351964-hduv0ur4 316 62 such such JJ cord-351964-hduv0ur4 316 63 as as IN cord-351964-hduv0ur4 316 64 virions virion NNS cord-351964-hduv0ur4 316 65 , , , cord-351964-hduv0ur4 316 66 even even RB cord-351964-hduv0ur4 316 67 though though IN cord-351964-hduv0ur4 316 68 normally normally RB cord-351964-hduv0ur4 316 69 they -PRON- PRP cord-351964-hduv0ur4 316 70 are be VBP cord-351964-hduv0ur4 316 71 never never RB cord-351964-hduv0ur4 316 72 called call VBN cord-351964-hduv0ur4 316 73 upon upon IN cord-351964-hduv0ur4 316 74 to to TO cord-351964-hduv0ur4 316 75 do do VB cord-351964-hduv0ur4 316 76 so so RB cord-351964-hduv0ur4 316 77 . . . cord-351964-hduv0ur4 317 1 It -PRON- PRP cord-351964-hduv0ur4 317 2 would would MD cord-351964-hduv0ur4 317 3 be be VB cord-351964-hduv0ur4 317 4 of of IN cord-351964-hduv0ur4 317 5 interest interest NN cord-351964-hduv0ur4 317 6 to to TO cord-351964-hduv0ur4 317 7 determine determine VB cord-351964-hduv0ur4 317 8 by by IN cord-351964-hduv0ur4 317 9 immunocytochemical immunocytochemical NNP cord-351964-hduv0ur4 317 10 means mean VBZ cord-351964-hduv0ur4 317 11 whether whether IN cord-351964-hduv0ur4 317 12 or or CC cord-351964-hduv0ur4 317 13 not not RB cord-351964-hduv0ur4 317 14 the the DT cord-351964-hduv0ur4 317 15 constitutive constitutive JJ cord-351964-hduv0ur4 317 16 pathway pathway NN cord-351964-hduv0ur4 317 17 vesicles vesicle NNS cord-351964-hduv0ur4 318 1 that that DT cord-351964-hduv0ur4 318 2 transport transport NN cord-351964-hduv0ur4 318 3 progeny progeny NN cord-351964-hduv0ur4 318 4 virions virion NNS cord-351964-hduv0ur4 318 5 from from IN cord-351964-hduv0ur4 318 6 the the DT cord-351964-hduv0ur4 318 7 trans trans NNP cord-351964-hduv0ur4 318 8 - - NNP cord-351964-hduv0ur4 318 9 Golgi Golgi NNP cord-351964-hduv0ur4 318 10 network network NN cord-351964-hduv0ur4 318 11 to to IN cord-351964-hduv0ur4 318 12 the the DT cord-351964-hduv0ur4 318 13 cell cell NN cord-351964-hduv0ur4 318 14 surface surface NN cord-351964-hduv0ur4 318 15 in in IN cord-351964-hduv0ur4 318 16 either either DT cord-351964-hduv0ur4 318 17 sac sac NN cord-351964-hduv0ur4 318 18 - - , cord-351964-hduv0ur4 318 19 or or CC cord-351964-hduv0ur4 318 20 AtT20 AtT20 NNP cord-351964-hduv0ur4 318 21 cells cell NNS cord-351964-hduv0ur4 318 22 also also RB cord-351964-hduv0ur4 318 23 contain contain VBP cord-351964-hduv0ur4 318 24 constitutively constitutively RB cord-351964-hduv0ur4 318 25 secreted secrete VBN cord-351964-hduv0ur4 318 26 cellular cellular JJ cord-351964-hduv0ur4 318 27 proteins protein NNS cord-351964-hduv0ur4 318 28 such such JJ cord-351964-hduv0ur4 318 29 as as IN cord-351964-hduv0ur4 318 30 laminin laminin NN cord-351964-hduv0ur4 318 31 . . . cord-351964-hduv0ur4 319 1 Such such JJ cord-351964-hduv0ur4 319 2 experiments experiment NNS cord-351964-hduv0ur4 319 3 are be VBP cord-351964-hduv0ur4 319 4 planned plan VBN cord-351964-hduv0ur4 319 5 . . . cord-351964-hduv0ur4 320 1 The the DT cord-351964-hduv0ur4 320 2 striking striking JJ cord-351964-hduv0ur4 320 3 and and CC cord-351964-hduv0ur4 320 4 irreversible irreversible JJ cord-351964-hduv0ur4 320 5 morphological morphological JJ cord-351964-hduv0ur4 320 6 maturation maturation NN cord-351964-hduv0ur4 320 7 of of IN cord-351964-hduv0ur4 320 8 coronavirions coronavirion NNS cord-351964-hduv0ur4 320 9 , , , cord-351964-hduv0ur4 320 10 which which WDT cord-351964-hduv0ur4 320 11 takes take VBZ cord-351964-hduv0ur4 320 12 place place NN cord-351964-hduv0ur4 320 13 during during IN cord-351964-hduv0ur4 320 14 their -PRON- PRP$ cord-351964-hduv0ur4 320 15 transit transit NN cord-351964-hduv0ur4 320 16 of of IN cord-351964-hduv0ur4 320 17 the the DT cord-351964-hduv0ur4 320 18 Golgi Golgi NNP cord-351964-hduv0ur4 320 19 apparatus apparatus NN cord-351964-hduv0ur4 320 20 in in IN cord-351964-hduv0ur4 320 21 several several JJ cord-351964-hduv0ur4 320 22 types type NNS cord-351964-hduv0ur4 320 23 of of IN cord-351964-hduv0ur4 320 24 host host NN cord-351964-hduv0ur4 320 25 cells cell NNS cord-351964-hduv0ur4 320 26 ( ( -LRB- cord-351964-hduv0ur4 320 27 see see VB cord-351964-hduv0ur4 320 28 Dubois Dubois NNP cord-351964-hduv0ur4 320 29 - - HYPH cord-351964-hduv0ur4 320 30 Dalcq Dalcq NNP cord-351964-hduv0ur4 320 31 et et NNP cord-351964-hduv0ur4 320 32 al al NNP cord-351964-hduv0ur4 320 33 . . NNP cord-351964-hduv0ur4 320 34 , , , cord-351964-hduv0ur4 320 35 1984 1984 CD cord-351964-hduv0ur4 320 36 ) ) -RRB- cord-351964-hduv0ur4 320 37 , , , cord-351964-hduv0ur4 320 38 occurs occur VBZ cord-351964-hduv0ur4 320 39 in in IN cord-351964-hduv0ur4 320 40 ART20 ART20 NNP cord-351964-hduv0ur4 320 41 cells cell NNS cord-351964-hduv0ur4 320 42 at at IN cord-351964-hduv0ur4 320 43 early early JJ cord-351964-hduv0ur4 320 44 times time NNS cord-351964-hduv0ur4 320 45 after after IN cord-351964-hduv0ur4 320 46 infection infection NN cord-351964-hduv0ur4 320 47 exclusively exclusively RB cord-351964-hduv0ur4 320 48 in in IN cord-351964-hduv0ur4 320 49 the the DT cord-351964-hduv0ur4 320 50 trans trans NNP cord-351964-hduv0ur4 320 51 - - NNP cord-351964-hduv0ur4 320 52 Golgi Golgi NNP cord-351964-hduv0ur4 320 53 network network NN cord-351964-hduv0ur4 320 54 . . . cord-351964-hduv0ur4 321 1 This this DT cord-351964-hduv0ur4 321 2 morphological morphological JJ cord-351964-hduv0ur4 321 3 maturation maturation NN cord-351964-hduv0ur4 321 4 is be VBZ cord-351964-hduv0ur4 321 5 not not RB cord-351964-hduv0ur4 321 6 , , , cord-351964-hduv0ur4 321 7 however however RB cord-351964-hduv0ur4 321 8 , , , cord-351964-hduv0ur4 321 9 inhibited inhibit VBN cord-351964-hduv0ur4 321 10 by by IN cord-351964-hduv0ur4 321 11 200 200 CD cord-351964-hduv0ur4 321 12 ~tM ~tM NNP cord-351964-hduv0ur4 321 13 chloroquine chloroquine NN cord-351964-hduv0ur4 321 14 , , , cord-351964-hduv0ur4 321 15 or or CC cord-351964-hduv0ur4 321 16 by by IN cord-351964-hduv0ur4 321 17 the the DT cord-351964-hduv0ur4 321 18 presence presence NN cord-351964-hduv0ur4 321 19 of of IN cord-351964-hduv0ur4 321 20 10 10 CD cord-351964-hduv0ur4 321 21 mM mm CD cord-351964-hduv0ur4 321 22 NI NI NNP cord-351964-hduv0ur4 321 23 - - HYPH cord-351964-hduv0ur4 321 24 hC1 hC1 NNP cord-351964-hduv0ur4 321 25 . . . cord-351964-hduv0ur4 322 1 It -PRON- PRP cord-351964-hduv0ur4 322 2 can can MD cord-351964-hduv0ur4 322 3 not not RB cord-351964-hduv0ur4 322 4 , , , cord-351964-hduv0ur4 322 5 therefore therefore RB cord-351964-hduv0ur4 322 6 , , , cord-351964-hduv0ur4 322 7 be be VB cord-351964-hduv0ur4 322 8 dependent dependent JJ cord-351964-hduv0ur4 322 9 on on IN cord-351964-hduv0ur4 322 10 the the DT cord-351964-hduv0ur4 322 11 lower low JJR cord-351964-hduv0ur4 322 12 pH ph NN cord-351964-hduv0ur4 322 13 in in IN cord-351964-hduv0ur4 322 14 the the DT cord-351964-hduv0ur4 322 15 transmost transmost NN cord-351964-hduv0ur4 322 16 cisterna cisterna NNP cord-351964-hduv0ur4 322 17 of of IN cord-351964-hduv0ur4 322 18 the the DT cord-351964-hduv0ur4 322 19 Golgi Golgi NNP cord-351964-hduv0ur4 322 20 apparatus apparatus NN cord-351964-hduv0ur4 322 21 compared compare VBN cord-351964-hduv0ur4 322 22 with with IN cord-351964-hduv0ur4 322 23 the the DT cord-351964-hduv0ur4 322 24 earlier early JJR cord-351964-hduv0ur4 322 25 compartments compartment NNS cord-351964-hduv0ur4 322 26 ( ( -LRB- cord-351964-hduv0ur4 322 27 Anderson Anderson NNP cord-351964-hduv0ur4 322 28 and and CC cord-351964-hduv0ur4 322 29 Pathak Pathak NNP cord-351964-hduv0ur4 322 30 , , , cord-351964-hduv0ur4 322 31 1985 1985 CD cord-351964-hduv0ur4 322 32 ) ) -RRB- cord-351964-hduv0ur4 322 33 . . . cord-351964-hduv0ur4 323 1 By by IN cord-351964-hduv0ur4 323 2 contrast contrast NN cord-351964-hduv0ur4 323 3 , , , cord-351964-hduv0ur4 323 4 condensation condensation NN cord-351964-hduv0ur4 323 5 of of IN cord-351964-hduv0ur4 323 6 secretory secretory JJ cord-351964-hduv0ur4 323 7 proteins protein NNS cord-351964-hduv0ur4 323 8 and and CC cord-351964-hduv0ur4 323 9 their -PRON- PRP$ cord-351964-hduv0ur4 323 10 packaging packaging NN cord-351964-hduv0ur4 323 11 into into IN cord-351964-hduv0ur4 323 12 secretory secretory JJ cord-351964-hduv0ur4 323 13 granules granule NNS cord-351964-hduv0ur4 323 14 in in IN cord-351964-hduv0ur4 323 15 these these DT cord-351964-hduv0ur4 323 16 cells cell NNS cord-351964-hduv0ur4 323 17 seems seem VBZ cord-351964-hduv0ur4 323 18 to to TO cord-351964-hduv0ur4 323 19 be be VB cord-351964-hduv0ur4 323 20 pH pH NNP cord-351964-hduv0ur4 323 21 dependent dependent JJ cord-351964-hduv0ur4 323 22 , , , cord-351964-hduv0ur4 323 23 since since IN cord-351964-hduv0ur4 323 24 Moore Moore NNP cord-351964-hduv0ur4 323 25 et et NNP cord-351964-hduv0ur4 323 26 al al NNP cord-351964-hduv0ur4 323 27 . . . cord-351964-hduv0ur4 324 1 ( ( -LRB- cord-351964-hduv0ur4 324 2 1983 1983 CD cord-351964-hduv0ur4 324 3 ) ) -RRB- cord-351964-hduv0ur4 324 4 established establish VBD cord-351964-hduv0ur4 324 5 that that IN cord-351964-hduv0ur4 324 6 200 200 CD cord-351964-hduv0ur4 324 7 ~tM ~tM NNP cord-351964-hduv0ur4 324 8 chloroquine chloroquine NN cord-351964-hduv0ur4 324 9 diverts divert VBZ cord-351964-hduv0ur4 324 10 ACTH ACTH NNP cord-351964-hduv0ur4 324 11 from from IN cord-351964-hduv0ur4 324 12 the the DT cord-351964-hduv0ur4 324 13 regulated regulate VBN cord-351964-hduv0ur4 324 14 to to IN cord-351964-hduv0ur4 324 15 the the DT cord-351964-hduv0ur4 324 16 constitutive constitutive JJ cord-351964-hduv0ur4 324 17 pathway pathway NN cord-351964-hduv0ur4 324 18 . . . cord-351964-hduv0ur4 325 1 Consistent consistent JJ cord-351964-hduv0ur4 325 2 with with IN cord-351964-hduv0ur4 325 3 these these DT cord-351964-hduv0ur4 325 4 results result NNS cord-351964-hduv0ur4 325 5 we -PRON- PRP cord-351964-hduv0ur4 325 6 observed observe VBD cord-351964-hduv0ur4 325 7 little little JJ cord-351964-hduv0ur4 325 8 , , , cord-351964-hduv0ur4 325 9 if if IN cord-351964-hduv0ur4 325 10 any any DT cord-351964-hduv0ur4 325 11 , , , cord-351964-hduv0ur4 325 12 condensing condense VBG cord-351964-hduv0ur4 325 13 secretory secretory JJ cord-351964-hduv0ur4 325 14 proteins protein NNS cord-351964-hduv0ur4 325 15 in in IN cord-351964-hduv0ur4 325 16 the the DT cord-351964-hduv0ur4 325 17 trans trans NNP cord-351964-hduv0ur4 325 18 - - NNP cord-351964-hduv0ur4 325 19 Golgi Golgi NNP cord-351964-hduv0ur4 325 20 network network NN cord-351964-hduv0ur4 325 21 of of IN cord-351964-hduv0ur4 325 22 either either CC cord-351964-hduv0ur4 325 23 uninfected uninfected JJ cord-351964-hduv0ur4 325 24 or or CC cord-351964-hduv0ur4 325 25 infected infected JJ cord-351964-hduv0ur4 325 26 ART20 ART20 NNP cord-351964-hduv0ur4 325 27 cells cell NNS cord-351964-hduv0ur4 325 28 after after IN cord-351964-hduv0ur4 325 29 their -PRON- PRP$ cord-351964-hduv0ur4 325 30 exposure exposure NN cord-351964-hduv0ur4 325 31 to to IN cord-351964-hduv0ur4 325 32 200 200 CD cord-351964-hduv0ur4 325 33 ~tM ~tM NNP cord-351964-hduv0ur4 325 34 chloroquine chloroquine NN cord-351964-hduv0ur4 325 35 or or CC cord-351964-hduv0ur4 325 36 10 10 CD cord-351964-hduv0ur4 325 37 mM mm CD cord-351964-hduv0ur4 325 38 NI NI NNP cord-351964-hduv0ur4 325 39 - - HYPH cord-351964-hduv0ur4 325 40 hCI hCI NNP cord-351964-hduv0ur4 325 41 for for IN cord-351964-hduv0ur4 325 42 2.5 2.5 CD cord-351964-hduv0ur4 325 43 h h NN cord-351964-hduv0ur4 325 44 ( ( -LRB- cord-351964-hduv0ur4 325 45 data datum NNS cord-351964-hduv0ur4 325 46 not not RB cord-351964-hduv0ur4 325 47 shown show VBN cord-351964-hduv0ur4 325 48 ) ) -RRB- cord-351964-hduv0ur4 325 49 . . . cord-351964-hduv0ur4 326 1 In in IN cord-351964-hduv0ur4 326 2 conclusion conclusion NN cord-351964-hduv0ur4 326 3 , , , cord-351964-hduv0ur4 326 4 the the DT cord-351964-hduv0ur4 326 5 trans trans NNP cord-351964-hduv0ur4 326 6 - - NNP cord-351964-hduv0ur4 326 7 Golgi Golgi NNP cord-351964-hduv0ur4 326 8 network network NN cord-351964-hduv0ur4 326 9 of of IN cord-351964-hduv0ur4 326 10 AtT20 AtT20 NNP cord-351964-hduv0ur4 326 11 cells cell NNS cord-351964-hduv0ur4 326 12 appears appear VBZ cord-351964-hduv0ur4 326 13 to to TO cord-351964-hduv0ur4 326 14 be be VB cord-351964-hduv0ur4 326 15 the the DT cord-351964-hduv0ur4 326 16 site site NN cord-351964-hduv0ur4 326 17 of of IN cord-351964-hduv0ur4 326 18 sorting sorting NN cord-351964-hduv0ur4 326 19 of of IN cord-351964-hduv0ur4 326 20 material material NN cord-351964-hduv0ur4 326 21 destined destine VBN cord-351964-hduv0ur4 326 22 for for IN cord-351964-hduv0ur4 326 23 the the DT cord-351964-hduv0ur4 326 24 constitutive constitutive JJ cord-351964-hduv0ur4 326 25 and and CC cord-351964-hduv0ur4 326 26 regulated regulated JJ cord-351964-hduv0ur4 326 27 exocytic exocytic JJ cord-351964-hduv0ur4 326 28 pathways pathway NNS cord-351964-hduv0ur4 326 29 , , , cord-351964-hduv0ur4 326 30 which which WDT cord-351964-hduv0ur4 326 31 diverge diverge VBP cord-351964-hduv0ur4 326 32 at at IN cord-351964-hduv0ur4 326 33 the the DT cord-351964-hduv0ur4 326 34 exit exit NN cord-351964-hduv0ur4 326 35 from from IN cord-351964-hduv0ur4 326 36 this this DT cord-351964-hduv0ur4 326 37 compartment compartment NN cord-351964-hduv0ur4 326 38 . . . cord-351964-hduv0ur4 327 1 Visualization visualization NN cord-351964-hduv0ur4 327 2 of of IN cord-351964-hduv0ur4 327 3 acidic acidic JJ cord-351964-hduv0ur4 327 4 organelles organelle NNS cord-351964-hduv0ur4 327 5 in in IN cord-351964-hduv0ur4 327 6 intact intact JJ cord-351964-hduv0ur4 327 7 cells cell NNS cord-351964-hduv0ur4 327 8 by by IN cord-351964-hduv0ur4 327 9 electron electron NN cord-351964-hduv0ur4 327 10 microscopy microscopy NN cord-351964-hduv0ur4 327 11 Vesicles Vesicles NNP cord-351964-hduv0ur4 327 12 and and CC cord-351964-hduv0ur4 327 13 cisternae cisternae NNP cord-351964-hduv0ur4 327 14 in in IN cord-351964-hduv0ur4 327 15 the the DT cord-351964-hduv0ur4 327 16 trans trans NNP cord-351964-hduv0ur4 327 17 Golgi Golgi NNP cord-351964-hduv0ur4 327 18 apparatus apparatus NN cord-351964-hduv0ur4 327 19 of of IN cord-351964-hduv0ur4 327 20 human human JJ cord-351964-hduv0ur4 327 21 fibroblasts fibroblast NNS cord-351964-hduv0ur4 327 22 are be VBP cord-351964-hduv0ur4 327 23 acidic acidic JJ cord-351964-hduv0ur4 327 24 compartments compartment NNS cord-351964-hduv0ur4 327 25 Antibody antibody NN cord-351964-hduv0ur4 327 26 - - HYPH cord-351964-hduv0ur4 327 27 induced induce VBN cord-351964-hduv0ur4 327 28 linkages linkage NNS cord-351964-hduv0ur4 327 29 of of IN cord-351964-hduv0ur4 327 30 plasma plasma NN cord-351964-hduv0ur4 327 31 membrane membrane NN cord-351964-hduv0ur4 327 32 proteins protein NNS cord-351964-hduv0ur4 327 33 to to IN cord-351964-hduv0ur4 327 34 intracellular intracellular JJ cord-351964-hduv0ur4 327 35 actomyosin actomyosin NN cord-351964-hduv0ur4 327 36 - - HYPH cord-351964-hduv0ur4 327 37 containing contain VBG cord-351964-hduv0ur4 327 38 filaments filament NNS cord-351964-hduv0ur4 327 39 in in IN cord-351964-hduv0ur4 327 40 cultured cultured JJ cord-351964-hduv0ur4 327 41 fibroblasts fibroblast NNS cord-351964-hduv0ur4 327 42 Phase phase NN cord-351964-hduv0ur4 327 43 separation separation NN cord-351964-hduv0ur4 327 44 of of IN cord-351964-hduv0ur4 327 45 integral integral JJ cord-351964-hduv0ur4 327 46 membrane membrane NN cord-351964-hduv0ur4 327 47 proteins protein NNS cord-351964-hduv0ur4 327 48 in in IN cord-351964-hduv0ur4 327 49 Triton Triton NNP cord-351964-hduv0ur4 327 50 X-114 X-114 VBZ cord-351964-hduv0ur4 327 51 solution solution NN cord-351964-hduv0ur4 328 1 The the DT cord-351964-hduv0ur4 328 2 exocrine exocrine JJ cord-351964-hduv0ur4 328 3 protein protein NN cord-351964-hduv0ur4 328 4 trypsinogen trypsinogen NNP cord-351964-hduv0ur4 328 5 is be VBZ cord-351964-hduv0ur4 328 6 targeted target VBN cord-351964-hduv0ur4 328 7 into into IN cord-351964-hduv0ur4 328 8 the the DT cord-351964-hduv0ur4 328 9 secretory secretory JJ cord-351964-hduv0ur4 328 10 granules granule NNS cord-351964-hduv0ur4 328 11 of of IN cord-351964-hduv0ur4 328 12 an an DT cord-351964-hduv0ur4 328 13 endocrine endocrine JJ cord-351964-hduv0ur4 328 14 cell cell NN cord-351964-hduv0ur4 328 15 line line NN cord-351964-hduv0ur4 328 16 : : : cord-351964-hduv0ur4 328 17 studies study NNS cord-351964-hduv0ur4 328 18 by by IN cord-351964-hduv0ur4 328 19 gene gene NN cord-351964-hduv0ur4 328 20 transfer transfer NN cord-351964-hduv0ur4 328 21 Biosynthesis Biosynthesis NNP cord-351964-hduv0ur4 328 22 of of IN cord-351964-hduv0ur4 328 23 N N NNP cord-351964-hduv0ur4 328 24 - - : cord-351964-hduv0ur4 328 25 and and CC cord-351964-hduv0ur4 328 26 O o NN cord-351964-hduv0ur4 328 27 - - HYPH cord-351964-hduv0ur4 328 28 linked link VBN cord-351964-hduv0ur4 328 29 oligosaccharides oligosaccharide NNS cord-351964-hduv0ur4 328 30 of of IN cord-351964-hduv0ur4 328 31 the the DT cord-351964-hduv0ur4 328 32 low low JJ cord-351964-hduv0ur4 328 33 density density NN cord-351964-hduv0ur4 328 34 lipoprotein lipoprotein NN cord-351964-hduv0ur4 328 35 receptor receptor NN cord-351964-hduv0ur4 328 36 Assembly Assembly NNP cord-351964-hduv0ur4 328 37 of of IN cord-351964-hduv0ur4 328 38 the the DT cord-351964-hduv0ur4 328 39 Coronvidrae Coronvidrae NNP cord-351964-hduv0ur4 328 40 An an DT cord-351964-hduv0ur4 328 41 enzymatic enzymatic JJ cord-351964-hduv0ur4 328 42 assay assay NN cord-351964-hduv0ur4 328 43 reveals reveal VBZ cord-351964-hduv0ur4 328 44 that that IN cord-351964-hduv0ur4 328 45 proteins protein NNS cord-351964-hduv0ur4 328 46 destined destine VBN cord-351964-hduv0ur4 328 47 for for IN cord-351964-hduv0ur4 328 48 the the DT cord-351964-hduv0ur4 328 49 apical apical JJ cord-351964-hduv0ur4 328 50 or or CC cord-351964-hduv0ur4 328 51 basolateral basolateral JJ cord-351964-hduv0ur4 328 52 domains domain NNS cord-351964-hduv0ur4 328 53 of of IN cord-351964-hduv0ur4 328 54 an an DT cord-351964-hduv0ur4 328 55 epithelial epithelial JJ cord-351964-hduv0ur4 328 56 cell cell NN cord-351964-hduv0ur4 328 57 share share NN cord-351964-hduv0ur4 328 58 the the DT cord-351964-hduv0ur4 328 59 same same JJ cord-351964-hduv0ur4 328 60 late late JJ cord-351964-hduv0ur4 328 61 Golgi Golgi NNP cord-351964-hduv0ur4 328 62 compartments compartment NNS cord-351964-hduv0ur4 328 63 Vesicular Vesicular NNP cord-351964-hduv0ur4 328 64 Stomatitis Stomatitis NNP cord-351964-hduv0ur4 328 65 virus virus NN cord-351964-hduv0ur4 328 66 infects infect VBZ cord-351964-hduv0ur4 328 67 and and CC cord-351964-hduv0ur4 328 68 matures mature VBZ cord-351964-hduv0ur4 328 69 only only RB cord-351964-hduv0ur4 328 70 through through IN cord-351964-hduv0ur4 328 71 the the DT cord-351964-hduv0ur4 328 72 basolateral basolateral JJ cord-351964-hduv0ur4 328 73 surface surface NN cord-351964-hduv0ur4 328 74 of of IN cord-351964-hduv0ur4 328 75 the the DT cord-351964-hduv0ur4 328 76 polarized polarized JJ cord-351964-hduv0ur4 328 77 epithelial epithelial JJ cord-351964-hduv0ur4 328 78 cell cell NN cord-351964-hduv0ur4 328 79 line line NN cord-351964-hduv0ur4 328 80 Efficient efficient JJ cord-351964-hduv0ur4 328 81 transport transport NN cord-351964-hduv0ur4 328 82 of of IN cord-351964-hduv0ur4 328 83 Semliki Semliki NNP cord-351964-hduv0ur4 328 84 Forest Forest NNP cord-351964-hduv0ur4 328 85 virus virus NN cord-351964-hduv0ur4 328 86 glycoproteins glycoprotein VBZ cord-351964-hduv0ur4 328 87 through through IN cord-351964-hduv0ur4 328 88 a a DT cord-351964-hduv0ur4 328 89 Golgi Golgi NNP cord-351964-hduv0ur4 328 90 complex complex NN cord-351964-hduv0ur4 328 91 morphologically morphologically RB cord-351964-hduv0ur4 328 92 altered alter VBN cord-351964-hduv0ur4 328 93 by by IN cord-351964-hduv0ur4 328 94 Uukuniemi Uukuniemi NNP cord-351964-hduv0ur4 328 95 virus virus NN cord-351964-hduv0ur4 328 96 glycoproteins glycoprotein NNS cord-351964-hduv0ur4 328 97 Passage passage NN cord-351964-hduv0ur4 328 98 of of IN cord-351964-hduv0ur4 328 99 viral viral JJ cord-351964-hduv0ur4 328 100 membrane membrane NN cord-351964-hduv0ur4 328 101 proteins protein NNS cord-351964-hduv0ur4 328 102 through through IN cord-351964-hduv0ur4 328 103 the the DT cord-351964-hduv0ur4 328 104 Golgi Golgi NNP cord-351964-hduv0ur4 328 105 complex complex NN cord-351964-hduv0ur4 329 1 The the DT cord-351964-hduv0ur4 329 2 trans trans NNP cord-351964-hduv0ur4 329 3 Golgi Golgi NNP cord-351964-hduv0ur4 329 4 network network NN cord-351964-hduv0ur4 329 5 ; ; : cord-351964-hduv0ur4 329 6 sorting sort VBG cord-351964-hduv0ur4 329 7 at at IN cord-351964-hduv0ur4 329 8 the the DT cord-351964-hduv0ur4 329 9 exit exit NN cord-351964-hduv0ur4 329 10 site site NN cord-351964-hduv0ur4 329 11 of of IN cord-351964-hduv0ur4 329 12 the the DT cord-351964-hduv0ur4 329 13 Golgi Golgi NNP cord-351964-hduv0ur4 329 14 complex complex NN cord-351964-hduv0ur4 329 15 Two two CD cord-351964-hduv0ur4 329 16 distinct distinct JJ cord-351964-hduv0ur4 329 17 intracellular intracellular JJ cord-351964-hduv0ur4 329 18 pathways pathway NNS cord-351964-hduv0ur4 329 19 transport transport VBP cord-351964-hduv0ur4 329 20 secretory secretory NN cord-351964-hduv0ur4 329 21 and and CC cord-351964-hduv0ur4 329 22 membrane membrane NN cord-351964-hduv0ur4 329 23 glycoproteins glycoprotein NNS cord-351964-hduv0ur4 329 24 to to IN cord-351964-hduv0ur4 329 25 the the DT cord-351964-hduv0ur4 329 26 surface surface NN cord-351964-hduv0ur4 329 27 of of IN cord-351964-hduv0ur4 329 28 pituitary pituitary JJ cord-351964-hduv0ur4 329 29 tumor tumor NN cord-351964-hduv0ur4 329 30 cells cell NNS cord-351964-hduv0ur4 329 31 Pathways pathway NNS cord-351964-hduv0ur4 329 32 of of IN cord-351964-hduv0ur4 329 33 protein protein NN cord-351964-hduv0ur4 329 34 secretion secretion NN cord-351964-hduv0ur4 329 35 in in IN cord-351964-hduv0ur4 329 36 eukaryotes eukaryote NNS cord-351964-hduv0ur4 329 37 Localization localization NN cord-351964-hduv0ur4 329 38 of of IN cord-351964-hduv0ur4 329 39 two two CD cord-351964-hduv0ur4 329 40 intracellular intracellular JJ cord-351964-hduv0ur4 329 41 forms form NNS cord-351964-hduv0ur4 329 42 of of IN cord-351964-hduv0ur4 329 43 the the DT cord-351964-hduv0ur4 329 44 vesicular vesicular JJ cord-351964-hduv0ur4 329 45 stomatitis stomatitis NN cord-351964-hduv0ur4 329 46 viral viral JJ cord-351964-hduv0ur4 329 47 glycoprotein glycoprotein NN cord-351964-hduv0ur4 329 48 Sorting sort VBG cord-351964-hduv0ur4 329 49 of of IN cord-351964-hduv0ur4 329 50 a a DT cord-351964-hduv0ur4 329 51 plasma plasma NN cord-351964-hduv0ur4 329 52 membrane membrane NN cord-351964-hduv0ur4 329 53 protein protein NN cord-351964-hduv0ur4 329 54 occurs occur VBZ cord-351964-hduv0ur4 329 55 before before IN cord-351964-hduv0ur4 329 56 it -PRON- PRP cord-351964-hduv0ur4 329 57 reaches reach VBZ cord-351964-hduv0ur4 329 58 the the DT cord-351964-hduv0ur4 329 59 cell cell NN cord-351964-hduv0ur4 329 60 surface surface NN cord-351964-hduv0ur4 329 61 in in IN cord-351964-hduv0ur4 329 62 cultured cultured JJ cord-351964-hduv0ur4 329 63 epithelial epithelial JJ cord-351964-hduv0ur4 329 64 cells cell NNS cord-351964-hduv0ur4 329 65 Biogenesis biogenesis NN cord-351964-hduv0ur4 329 66 of of IN cord-351964-hduv0ur4 329 67 epithelial epithelial JJ cord-351964-hduv0ur4 329 68 cell cell NN cord-351964-hduv0ur4 329 69 polarity polarity NN cord-351964-hduv0ur4 329 70 : : : cord-351964-hduv0ur4 329 71 intracellular intracellular JJ cord-351964-hduv0ur4 329 72 sorting sorting NN cord-351964-hduv0ur4 329 73 and and CC cord-351964-hduv0ur4 329 74 vectorial vectorial JJ cord-351964-hduv0ur4 329 75 exocytosis exocytosis NN cord-351964-hduv0ur4 329 76 of of IN cord-351964-hduv0ur4 329 77 an an DT cord-351964-hduv0ur4 329 78 apical apical JJ cord-351964-hduv0ur4 329 79 plasma plasma NN cord-351964-hduv0ur4 329 80 membrane membrane NN cord-351964-hduv0ur4 329 81 glycoprotein glycoprotein NN cord-351964-hduv0ur4 329 82 Chloroquine Chloroquine NNP cord-351964-hduv0ur4 329 83 diverts divert VBZ cord-351964-hduv0ur4 329 84 ACTH ACTH NNP cord-351964-hduv0ur4 329 85 from from IN cord-351964-hduv0ur4 329 86 a a DT cord-351964-hduv0ur4 329 87 regulated regulate VBN cord-351964-hduv0ur4 329 88 to to IN cord-351964-hduv0ur4 329 89 a a DT cord-351964-hduv0ur4 329 90 constitutive constitutive JJ cord-351964-hduv0ur4 329 91 pathway pathway NN cord-351964-hduv0ur4 329 92 in in IN cord-351964-hduv0ur4 329 93 ART20 ART20 NNP cord-351964-hduv0ur4 329 94 cells cell NNS cord-351964-hduv0ur4 329 95 Secretory secretory JJ cord-351964-hduv0ur4 329 96 protein protein NN cord-351964-hduv0ur4 329 97 targeting target VBG cord-351964-hduv0ur4 329 98 in in IN cord-351964-hduv0ur4 329 99 a a DT cord-351964-hduv0ur4 329 100 pituitary pituitary JJ cord-351964-hduv0ur4 329 101 cell cell NN cord-351964-hduv0ur4 329 102 line line NN cord-351964-hduv0ur4 329 103 : : : cord-351964-hduv0ur4 329 104 differential differential JJ cord-351964-hduv0ur4 329 105 transport transport NN cord-351964-hduv0ur4 329 106 of of IN cord-351964-hduv0ur4 329 107 foreign foreign JJ cord-351964-hduv0ur4 329 108 secretory secretory NN cord-351964-hduv0ur4 329 109 proteins protein NNS cord-351964-hduv0ur4 329 110 to to IN cord-351964-hduv0ur4 329 111 distinct distinct JJ cord-351964-hduv0ur4 329 112 secretory secretory JJ cord-351964-hduv0ur4 329 113 pathways pathway NNS cord-351964-hduv0ur4 330 1 The the DT cord-351964-hduv0ur4 330 2 carbohydrates carbohydrate NNS cord-351964-hduv0ur4 330 3 of of IN cord-351964-hduv0ur4 330 4 the the DT cord-351964-hduv0ur4 330 5 O o NN cord-351964-hduv0ur4 330 6 - - HYPH cord-351964-hduv0ur4 330 7 glycosidically glycosidically RB cord-351964-hduv0ur4 330 8 linked link VBN cord-351964-hduv0ur4 330 9 oligosaccharides oligosaccharide NNS cord-351964-hduv0ur4 330 10 of of IN cord-351964-hduv0ur4 330 11 glycoprotein glycoprotein NN cord-351964-hduv0ur4 330 12 El El NNP cord-351964-hduv0ur4 330 13 Coronavirus Coronavirus NNP cord-351964-hduv0ur4 330 14 glycoprotein glycoprotein NN cord-351964-hduv0ur4 330 15 El El NNP cord-351964-hduv0ur4 330 16 , , , cord-351964-hduv0ur4 330 17 a a DT cord-351964-hduv0ur4 330 18 new new JJ cord-351964-hduv0ur4 330 19 type type NN cord-351964-hduv0ur4 330 20 of of IN cord-351964-hduv0ur4 330 21 viral viral JJ cord-351964-hduv0ur4 330 22 glycoprotein glycoprotein NN cord-351964-hduv0ur4 330 23 Intracellular Intracellular NNP cord-351964-hduv0ur4 330 24 sorting sort VBG cord-351964-hduv0ur4 330 25 and and CC cord-351964-hduv0ur4 330 26 basolateral basolateral JJ cord-351964-hduv0ur4 330 27 appearance appearance NN cord-351964-hduv0ur4 330 28 of of IN cord-351964-hduv0ur4 330 29 the the DT cord-351964-hduv0ur4 330 30 G g NN cord-351964-hduv0ur4 330 31 protein protein NN cord-351964-hduv0ur4 330 32 of of IN cord-351964-hduv0ur4 330 33 vesicular vesicular JJ cord-351964-hduv0ur4 330 34 stomatitis stomatitis NN cord-351964-hduv0ur4 330 35 virus virus NN cord-351964-hduv0ur4 330 36 in in IN cord-351964-hduv0ur4 330 37 MDCK MDCK NNP cord-351964-hduv0ur4 330 38 cells cell NNS cord-351964-hduv0ur4 330 39 Use Use NNP cord-351964-hduv0ur4 330 40 of of IN cord-351964-hduv0ur4 330 41 immunocytocbemical immunocytocbemical JJ cord-351964-hduv0ur4 330 42 techniques technique NNS cord-351964-hduv0ur4 330 43 in in IN cord-351964-hduv0ur4 330 44 studying study VBG cord-351964-hduv0ur4 330 45 the the DT cord-351964-hduv0ur4 330 46 biogenesis biogenesis NN cord-351964-hduv0ur4 330 47 of of IN cord-351964-hduv0ur4 330 48 cell cell NN cord-351964-hduv0ur4 330 49 surfaces surface NNS cord-351964-hduv0ur4 330 50 in in IN cord-351964-hduv0ur4 330 51 polarised polarised JJ cord-351964-hduv0ur4 330 52 epithelia epithelium NNS cord-351964-hduv0ur4 330 53 Viral viral JJ cord-351964-hduv0ur4 330 54 glycoprotein glycoprotein NN cord-351964-hduv0ur4 330 55 destined destine VBN cord-351964-hduv0ur4 330 56 for for IN cord-351964-hduv0ur4 330 57 apical apical JJ cord-351964-hduv0ur4 330 58 or or CC cord-351964-hduv0ur4 330 59 basolateral basolateral JJ cord-351964-hduv0ur4 330 60 plasma plasma NN cord-351964-hduv0ur4 330 61 membrane membrane NN cord-351964-hduv0ur4 330 62 domains domain NNS cord-351964-hduv0ur4 330 63 traverse traverse VBP cord-351964-hduv0ur4 330 64 the the DT cord-351964-hduv0ur4 330 65 same same JJ cord-351964-hduv0ur4 330 66 Golgi Golgi NNP cord-351964-hduv0ur4 330 67 apparatus apparatus NN cord-351964-hduv0ur4 330 68 during during IN cord-351964-hduv0ur4 330 69 their -PRON- PRP$ cord-351964-hduv0ur4 330 70 intracellufar intracellufar VBN cord-351964-hduv0ur4 330 71 transport transport NN cord-351964-hduv0ur4 330 72 in in IN cord-351964-hduv0ur4 330 73 doubly doubly RB cord-351964-hduv0ur4 330 74 infected infect VBN cord-351964-hduv0ur4 330 75 MDCK MDCK NNP cord-351964-hduv0ur4 330 76 cells cell NNS cord-351964-hduv0ur4 330 77 Identification identification NN cord-351964-hduv0ur4 330 78 of of IN cord-351964-hduv0ur4 330 79 two two CD cord-351964-hduv0ur4 330 80 epitopes epitope NNS cord-351964-hduv0ur4 330 81 in in IN cord-351964-hduv0ur4 330 82 the the DT cord-351964-hduv0ur4 330 83 carboxy carboxy JJ cord-351964-hduv0ur4 330 84 terminal terminal NN cord-351964-hduv0ur4 330 85 15 15 CD cord-351964-hduv0ur4 330 86 amino amino JJ cord-351964-hduv0ur4 330 87 acids acid NNS cord-351964-hduv0ur4 330 88 of of IN cord-351964-hduv0ur4 330 89 the the DT cord-351964-hduv0ur4 330 90 El El NNP cord-351964-hduv0ur4 330 91 glycoprotein glycoprotein NN cord-351964-hduv0ur4 330 92 of of IN cord-351964-hduv0ur4 330 93 Murine Murine NNP cord-351964-hduv0ur4 330 94 Hepatitis Hepatitis NNP cord-351964-hduv0ur4 330 95 virus virus NN cord-351964-hduv0ur4 330 96 A59 A59 NNP cord-351964-hduv0ur4 330 97 by by IN cord-351964-hduv0ur4 330 98 using use VBG cord-351964-hduv0ur4 330 99 hybrid hybrid JJ cord-351964-hduv0ur4 330 100 proteins protein NNS cord-351964-hduv0ur4 330 101 Infection infection NN cord-351964-hduv0ur4 330 102 of of IN cord-351964-hduv0ur4 330 103 ART20 ART20 NNP cord-351964-hduv0ur4 330 104 murine murine JJ cord-351964-hduv0ur4 330 105 pituitary pituitary NN cord-351964-hduv0ur4 330 106 tumour tumour NN cord-351964-hduv0ur4 330 107 cells cell NNS cord-351964-hduv0ur4 330 108 by by IN cord-351964-hduv0ur4 330 109 mouse mouse NN cord-351964-hduv0ur4 330 110 hepatitis hepatitis NNP cord-351964-hduv0ur4 330 111 virus virus NN cord-351964-hduv0ur4 331 1 strain strain VB cord-351964-hduv0ur4 331 2 A59 A59 NNP cord-351964-hduv0ur4 332 1 : : : cord-351964-hduv0ur4 332 2 virus virus NN cord-351964-hduv0ur4 332 3 budding budding NN cord-351964-hduv0ur4 332 4 is be VBZ cord-351964-hduv0ur4 332 5 restricted restrict VBN cord-351964-hduv0ur4 332 6 to to IN cord-351964-hduv0ur4 332 7 the the DT cord-351964-hduv0ur4 332 8 Golgi Golgi NNP cord-351964-hduv0ur4 332 9 region region NN cord-351964-hduv0ur4 332 10 Clathrin Clathrin NNP cord-351964-hduv0ur4 332 11 - - HYPH cord-351964-hduv0ur4 332 12 coated coat VBN cord-351964-hduv0ur4 332 13 vesicular vesicular JJ cord-351964-hduv0ur4 332 14 transport transport NN cord-351964-hduv0ur4 332 15 of of IN cord-351964-hduv0ur4 332 16 secretory secretory JJ cord-351964-hduv0ur4 332 17 proteins protein NNS cord-351964-hduv0ur4 332 18 during during IN cord-351964-hduv0ur4 332 19 the the DT cord-351964-hduv0ur4 332 20 formation formation NN cord-351964-hduv0ur4 332 21 of of IN cord-351964-hduv0ur4 332 22 ACTH ACTH NNP cord-351964-hduv0ur4 332 23 - - HYPH cord-351964-hduv0ur4 332 24 containing contain VBG cord-351964-hduv0ur4 332 25 secretory secretory JJ cord-351964-hduv0ur4 332 26 granules granule NNS cord-351964-hduv0ur4 332 27 in in IN cord-351964-hduv0ur4 332 28 ART20 art20 NN cord-351964-hduv0ur4 332 29 cells cell NNS cord-351964-hduv0ur4 332 30 Replication replication NN cord-351964-hduv0ur4 332 31 of of IN cord-351964-hduv0ur4 332 32 coronavirus coronavirus NN cord-351964-hduv0ur4 332 33 MHV mhv NN cord-351964-hduv0ur4 332 34 - - HYPH cord-351964-hduv0ur4 332 35 A59 A59 NNP cord-351964-hduv0ur4 332 36 in in IN cord-351964-hduv0ur4 332 37 sac sac NN cord-351964-hduv0ur4 332 38 - - HYPH cord-351964-hduv0ur4 332 39 cells cell NNS cord-351964-hduv0ur4 332 40 : : : cord-351964-hduv0ur4 332 41 determination determination NN cord-351964-hduv0ur4 332 42 of of IN cord-351964-hduv0ur4 332 43 the the DT cord-351964-hduv0ur4 332 44 first first JJ cord-351964-hduv0ur4 332 45 site site NN cord-351964-hduv0ur4 332 46 of of IN cord-351964-hduv0ur4 332 47 budding budding NN cord-351964-hduv0ur4 332 48 of of IN cord-351964-hduv0ur4 332 49 progeny progeny JJ cord-351964-hduv0ur4 332 50 virus virus NN cord-351964-hduv0ur4 333 1 We -PRON- PRP cord-351964-hduv0ur4 333 2 thank thank VBP cord-351964-hduv0ur4 333 3 our -PRON- PRP$ cord-351964-hduv0ur4 333 4 colleagues colleague NNS cord-351964-hduv0ur4 333 5 Stella Stella NNP cord-351964-hduv0ur4 333 6 Hurtiey Hurtiey NNP cord-351964-hduv0ur4 333 7 , , , cord-351964-hduv0ur4 333 8 Jean Jean NNP cord-351964-hduv0ur4 333 9 Gruenberg Gruenberg NNP cord-351964-hduv0ur4 333 10 , , , cord-351964-hduv0ur4 333 11 and and CC cord-351964-hduv0ur4 333 12 Gareth Gareth NNP cord-351964-hduv0ur4 333 13 Griffiths Griffiths NNP cord-351964-hduv0ur4 333 14 ( ( -LRB- cord-351964-hduv0ur4 333 15 European European NNP cord-351964-hduv0ur4 333 16 Molecular Molecular NNP cord-351964-hduv0ur4 333 17 Biological Biological NNP cord-351964-hduv0ur4 333 18 Laboratory Laboratory NNP cord-351964-hduv0ur4 333 19 , , , cord-351964-hduv0ur4 333 20 Heidelberg Heidelberg NNP cord-351964-hduv0ur4 333 21 ) ) -RRB- cord-351964-hduv0ur4 333 22 for for IN cord-351964-hduv0ur4 333 23 their -PRON- PRP$ cord-351964-hduv0ur4 333 24 constructive constructive JJ cord-351964-hduv0ur4 333 25 comments comment NNS cord-351964-hduv0ur4 333 26 on on IN cord-351964-hduv0ur4 333 27 the the DT cord-351964-hduv0ur4 333 28 manuscript manuscript NN cord-351964-hduv0ur4 333 29 , , , cord-351964-hduv0ur4 333 30 and and CC cord-351964-hduv0ur4 333 31 Ines Ines NNP cord-351964-hduv0ur4 333 32 Benner Benner NNP cord-351964-hduv0ur4 333 33 who who WP cord-351964-hduv0ur4 333 34 patiently patiently RB cord-351964-hduv0ur4 333 35 and and CC cord-351964-hduv0ur4 333 36 skillfully skillfully RB cord-351964-hduv0ur4 333 37 typed type VBD cord-351964-hduv0ur4 333 38 this this DT cord-351964-hduv0ur4 333 39 manuscript manuscript NN cord-351964-hduv0ur4 333 40 . . . cord-351964-hduv0ur4 334 1 Received receive VBN cord-351964-hduv0ur4 334 2 for for IN cord-351964-hduv0ur4 334 3 publication publication NN cord-351964-hduv0ur4 334 4 19 19 CD cord-351964-hduv0ur4 334 5 March March NNP cord-351964-hduv0ur4 334 6 1987 1987 CD cord-351964-hduv0ur4 334 7 , , , cord-351964-hduv0ur4 334 8 and and CC cord-351964-hduv0ur4 334 9 in in IN cord-351964-hduv0ur4 334 10 revised revise VBN cord-351964-hduv0ur4 334 11 form form NN cord-351964-hduv0ur4 334 12 11 11 CD cord-351964-hduv0ur4 334 13 May May NNP cord-351964-hduv0ur4 334 14 1987 1987 CD cord-351964-hduv0ur4 334 15 . . .