id sid tid token lemma pos cord-329553-93tbgn2b 1 1 key key JJ cord-329553-93tbgn2b 1 2 : : : cord-329553-93tbgn2b 1 3 cord-329553 cord-329553 NNP cord-329553-93tbgn2b 1 4 - - HYPH cord-329553-93tbgn2b 1 5 93tbgn2b 93tbgn2b CD cord-329553-93tbgn2b 1 6 authors author NNS cord-329553-93tbgn2b 1 7 : : : cord-329553-93tbgn2b 2 1 nan nan NNP cord-329553-93tbgn2b 2 2 title title NN cord-329553-93tbgn2b 2 3 : : : cord-329553-93tbgn2b 3 1 The the DT cord-329553-93tbgn2b 3 2 17-residue 17-residue CD cord-329553-93tbgn2b 3 3 transmembrane transmembrane NN cord-329553-93tbgn2b 3 4 domain domain NN cord-329553-93tbgn2b 3 5 of of IN cord-329553-93tbgn2b 3 6 beta beta NN cord-329553-93tbgn2b 3 7 - - HYPH cord-329553-93tbgn2b 3 8 galactoside galactoside JJ cord-329553-93tbgn2b 3 9 alpha alpha NN cord-329553-93tbgn2b 3 10 2,6- 2,6- CD cord-329553-93tbgn2b 3 11 sialyltransferase sialyltransferase NNP cord-329553-93tbgn2b 3 12 is be VBZ cord-329553-93tbgn2b 3 13 sufficient sufficient JJ cord-329553-93tbgn2b 3 14 for for IN cord-329553-93tbgn2b 3 15 Golgi Golgi NNP cord-329553-93tbgn2b 3 16 retention retention NN cord-329553-93tbgn2b 3 17 date date NN cord-329553-93tbgn2b 4 1 : : : cord-329553-93tbgn2b 4 2 1992 1992 CD cord-329553-93tbgn2b 4 3 - - HYPH cord-329553-93tbgn2b 4 4 04 04 CD cord-329553-93tbgn2b 4 5 - - HYPH cord-329553-93tbgn2b 4 6 02 02 CD cord-329553-93tbgn2b 4 7 journal journal NN cord-329553-93tbgn2b 4 8 : : : cord-329553-93tbgn2b 5 1 J J NNP cord-329553-93tbgn2b 5 2 Cell Cell NNP cord-329553-93tbgn2b 5 3 Biol Biol NNP cord-329553-93tbgn2b 5 4 DOI DOI NNP cord-329553-93tbgn2b 5 5 : : : cord-329553-93tbgn2b 6 1 nan nan NNP cord-329553-93tbgn2b 6 2 sha sha NNP cord-329553-93tbgn2b 6 3 : : : cord-329553-93tbgn2b 6 4 885674f9e88e504ac2fed625ec9c64fdc3ad71f0 885674f9e88e504ac2fed625ec9c64fdc3ad71f0 CD cord-329553-93tbgn2b 6 5 doc_id doc_id CD cord-329553-93tbgn2b 6 6 : : : cord-329553-93tbgn2b 6 7 329553 329553 CD cord-329553-93tbgn2b 6 8 cord_uid cord_uid NNS cord-329553-93tbgn2b 6 9 : : : cord-329553-93tbgn2b 7 1 93tbgn2b 93tbgn2b CD cord-329553-93tbgn2b 7 2 beta beta NN cord-329553-93tbgn2b 7 3 - - HYPH cord-329553-93tbgn2b 7 4 Galactoside Galactoside NNP cord-329553-93tbgn2b 7 5 alpha alpha NN cord-329553-93tbgn2b 7 6 2,6-sialyltransferase 2,6-sialyltransferase CD cord-329553-93tbgn2b 8 1 ( ( -LRB- cord-329553-93tbgn2b 8 2 ST ST NNP cord-329553-93tbgn2b 8 3 ) ) -RRB- cord-329553-93tbgn2b 8 4 is be VBZ cord-329553-93tbgn2b 8 5 a a DT cord-329553-93tbgn2b 8 6 type type NN cord-329553-93tbgn2b 8 7 II ii CD cord-329553-93tbgn2b 8 8 integral integral JJ cord-329553-93tbgn2b 8 9 membrane membrane NN cord-329553-93tbgn2b 8 10 protein protein NN cord-329553-93tbgn2b 8 11 of of IN cord-329553-93tbgn2b 8 12 the the DT cord-329553-93tbgn2b 8 13 Golgi Golgi NNP cord-329553-93tbgn2b 8 14 apparatus apparatus NN cord-329553-93tbgn2b 8 15 involved involve VBN cord-329553-93tbgn2b 8 16 in in IN cord-329553-93tbgn2b 8 17 the the DT cord-329553-93tbgn2b 8 18 sialylation sialylation NN cord-329553-93tbgn2b 8 19 of of IN cord-329553-93tbgn2b 8 20 N n NN cord-329553-93tbgn2b 8 21 - - HYPH cord-329553-93tbgn2b 8 22 linked link VBN cord-329553-93tbgn2b 8 23 glycans glycan NNS cord-329553-93tbgn2b 8 24 . . . cord-329553-93tbgn2b 9 1 A a DT cord-329553-93tbgn2b 9 2 series series NN cord-329553-93tbgn2b 9 3 of of IN cord-329553-93tbgn2b 9 4 experiments experiment NNS cord-329553-93tbgn2b 9 5 has have VBZ cord-329553-93tbgn2b 9 6 shown show VBN cord-329553-93tbgn2b 9 7 that that IN cord-329553-93tbgn2b 9 8 the the DT cord-329553-93tbgn2b 9 9 17-residue 17-residue CD cord-329553-93tbgn2b 9 10 transmembrane transmembrane NN cord-329553-93tbgn2b 9 11 domain domain NN cord-329553-93tbgn2b 9 12 of of IN cord-329553-93tbgn2b 9 13 ST ST NNP cord-329553-93tbgn2b 9 14 is be VBZ cord-329553-93tbgn2b 9 15 sufficient sufficient JJ cord-329553-93tbgn2b 9 16 to to TO cord-329553-93tbgn2b 9 17 confer confer VB cord-329553-93tbgn2b 9 18 localization localization NN cord-329553-93tbgn2b 9 19 to to IN cord-329553-93tbgn2b 9 20 the the DT cord-329553-93tbgn2b 9 21 Golgi Golgi NNP cord-329553-93tbgn2b 9 22 apparatus apparatus NN cord-329553-93tbgn2b 9 23 when when WRB cord-329553-93tbgn2b 9 24 transferred transfer VBN cord-329553-93tbgn2b 9 25 to to IN cord-329553-93tbgn2b 9 26 the the DT cord-329553-93tbgn2b 9 27 corresponding corresponding JJ cord-329553-93tbgn2b 9 28 region region NN cord-329553-93tbgn2b 9 29 of of IN cord-329553-93tbgn2b 9 30 a a DT cord-329553-93tbgn2b 9 31 cell cell NN cord-329553-93tbgn2b 9 32 surface surface NN cord-329553-93tbgn2b 9 33 type type NN cord-329553-93tbgn2b 9 34 II II NNP cord-329553-93tbgn2b 9 35 integral integral JJ cord-329553-93tbgn2b 9 36 membrane membrane NN cord-329553-93tbgn2b 9 37 protein protein NN cord-329553-93tbgn2b 9 38 . . . cord-329553-93tbgn2b 10 1 Lectin Lectin NNP cord-329553-93tbgn2b 10 2 affinity affinity NN cord-329553-93tbgn2b 10 3 chromatography chromatography NN cord-329553-93tbgn2b 10 4 of of IN cord-329553-93tbgn2b 10 5 chimeric chimeric JJ cord-329553-93tbgn2b 10 6 proteins protein NNS cord-329553-93tbgn2b 10 7 bearing bear VBG cord-329553-93tbgn2b 10 8 this this DT cord-329553-93tbgn2b 10 9 17-residue 17-residue CD cord-329553-93tbgn2b 10 10 sequence sequence NN cord-329553-93tbgn2b 10 11 suggests suggest VBZ cord-329553-93tbgn2b 10 12 that that IN cord-329553-93tbgn2b 10 13 these these DT cord-329553-93tbgn2b 10 14 chimeric chimeric JJ cord-329553-93tbgn2b 10 15 proteins protein NNS cord-329553-93tbgn2b 10 16 are be VBP cord-329553-93tbgn2b 10 17 localized localize VBN cord-329553-93tbgn2b 10 18 in in IN cord-329553-93tbgn2b 10 19 the the DT cord-329553-93tbgn2b 10 20 trans trans NNP cord-329553-93tbgn2b 10 21 - - NNP cord-329553-93tbgn2b 10 22 Golgi Golgi NNP cord-329553-93tbgn2b 10 23 cisternae cisternae NN cord-329553-93tbgn2b 11 1 and/or and/or CC cord-329553-93tbgn2b 11 2 trans trans NNP cord-329553-93tbgn2b 11 3 - - NNP cord-329553-93tbgn2b 11 4 Golgi Golgi NNP cord-329553-93tbgn2b 11 5 network network NN cord-329553-93tbgn2b 11 6 . . . cord-329553-93tbgn2b 12 1 Further further JJ cord-329553-93tbgn2b 12 2 experiments experiment NNS cord-329553-93tbgn2b 12 3 suggest suggest VBP cord-329553-93tbgn2b 12 4 that that IN cord-329553-93tbgn2b 12 5 this this DT cord-329553-93tbgn2b 12 6 17-residue 17-residue CD cord-329553-93tbgn2b 12 7 sequence sequence NN cord-329553-93tbgn2b 12 8 functions function NNS cord-329553-93tbgn2b 12 9 as as IN cord-329553-93tbgn2b 12 10 a a DT cord-329553-93tbgn2b 12 11 retention retention NN cord-329553-93tbgn2b 12 12 signal signal NN cord-329553-93tbgn2b 12 13 for for IN cord-329553-93tbgn2b 12 14 the the DT cord-329553-93tbgn2b 12 15 Golgi Golgi NNP cord-329553-93tbgn2b 12 16 apparatus apparatus NN cord-329553-93tbgn2b 12 17 . . . cord-329553-93tbgn2b 13 1 transferase transferase NNP cord-329553-93tbgn2b 13 2 I I NNP cord-329553-93tbgn2b 13 3 ( ( -LRB- cord-329553-93tbgn2b 13 4 NTI NTI NNP cord-329553-93tbgn2b 13 5 ) ) -RRB- cord-329553-93tbgn2b 13 6 , , , cord-329553-93tbgn2b 13 7 ß-1,4-galactosyltransferase ß-1,4-galactosyltransferase NNP cord-329553-93tbgn2b 13 8 ( ( -LRB- cord-329553-93tbgn2b 13 9 GT GT NNP cord-329553-93tbgn2b 13 10 ) ) -RRB- cord-329553-93tbgn2b 13 11 , , , cord-329553-93tbgn2b 13 12 and and CC cord-329553-93tbgn2b 13 13 c12,6-sialyltransferase c12,6-sialyltransferase XX cord-329553-93tbgn2b 14 1 ( ( -LRB- cord-329553-93tbgn2b 14 2 ST)have ST)have NNP cord-329553-93tbgn2b 14 3 beenlocalizedto beenlocalizedto NNP cord-329553-93tbgn2b 14 4 themedial- themedial- NN cord-329553-93tbgn2b 14 5 , , , cord-329553-93tbgn2b 14 6 trams tram NNS cord-329553-93tbgn2b 14 7 - - : cord-329553-93tbgn2b 14 8 cisternae cisternae JJ cord-329553-93tbgn2b 14 9 , , , cord-329553-93tbgn2b 14 10 and and CC cord-329553-93tbgn2b 14 11 the the DT cord-329553-93tbgn2b 14 12 trams tram NNS cord-329553-93tbgn2b 14 13 - - HYPH cord-329553-93tbgn2b 14 14 cistemae cistemae NNP cord-329553-93tbgn2b 14 15 / / SYM cord-329553-93tbgn2b 14 16 TGN TGN NNP cord-329553-93tbgn2b 14 17 , , , cord-329553-93tbgn2b 14 18 respectively respectively RB cord-329553-93tbgn2b 15 1 ( ( -LRB- cord-329553-93tbgn2b 15 2 Dunphy Dunphy NNP cord-329553-93tbgn2b 15 3 et et FW cord-329553-93tbgn2b 15 4 al al NNP cord-329553-93tbgn2b 15 5 . . NNP cord-329553-93tbgn2b 15 6 , , , cord-329553-93tbgn2b 15 7 1985 1985 CD cord-329553-93tbgn2b 15 8 ; ; : cord-329553-93tbgn2b 16 1 Roth Roth NNP cord-329553-93tbgn2b 16 2 and and CC cord-329553-93tbgn2b 16 3 Berger Berger NNP cord-329553-93tbgn2b 16 4 , , , cord-329553-93tbgn2b 16 5 1982 1982 CD cord-329553-93tbgn2b 16 6 ; ; : cord-329553-93tbgn2b 16 7 Roth Roth NNP cord-329553-93tbgn2b 16 8 et et FW cord-329553-93tbgn2b 16 9 al al NNP cord-329553-93tbgn2b 16 10 . . NNP cord-329553-93tbgn2b 16 11 , , , cord-329553-93tbgn2b 16 12 1985 1985 CD cord-329553-93tbgn2b 16 13 ; ; : cord-329553-93tbgn2b 16 14 Chege Chege NNP cord-329553-93tbgn2b 16 15 and and CC cord-329553-93tbgn2b 16 16 Pfeffer Pfeffer NNP cord-329553-93tbgn2b 16 17 , , , cord-329553-93tbgn2b 16 18 1990 1990 CD cord-329553-93tbgn2b 16 19 ) ) -RRB- cord-329553-93tbgn2b 16 20 . . . cord-329553-93tbgn2b 17 1 Despite despite IN cord-329553-93tbgn2b 17 2 our -PRON- PRP$ cord-329553-93tbgn2b 17 3 current current JJ cord-329553-93tbgn2b 17 4 knowledge knowledge NN cord-329553-93tbgn2b 17 5 about about IN cord-329553-93tbgn2b 17 6 the the DT cord-329553-93tbgn2b 17 7 morphology morphology NN cord-329553-93tbgn2b 17 8 , , , cord-329553-93tbgn2b 17 9 organization organization NN cord-329553-93tbgn2b 17 10 , , , cord-329553-93tbgn2b 17 11 function function NN cord-329553-93tbgn2b 17 12 , , , cord-329553-93tbgn2b 17 13 and and CC cord-329553-93tbgn2b 17 14 composition composition NN cord-329553-93tbgn2b 17 15 of of IN cord-329553-93tbgn2b 17 16 the the DT cord-329553-93tbgn2b 17 17 Golgi Golgi NNP cord-329553-93tbgn2b 17 18 apparatus apparatus NN cord-329553-93tbgn2b 17 19 , , , cord-329553-93tbgn2b 17 20 the the DT cord-329553-93tbgn2b 17 21 mechanism mechanism NN cord-329553-93tbgn2b 17 22 for for IN cord-329553-93tbgn2b 17 23 establishing establish VBG cord-329553-93tbgn2b 17 24 and and CC cord-329553-93tbgn2b 17 25 maintaining maintain VBG cord-329553-93tbgn2b 17 26 the the DT cord-329553-93tbgn2b 17 27 highly highly RB cord-329553-93tbgn2b 17 28 ordered order VBN cord-329553-93tbgn2b 17 29 Golgi Golgi NNP cord-329553-93tbgn2b 17 30 structure structure NN cord-329553-93tbgn2b 17 31 is be VBZ cord-329553-93tbgn2b 17 32 poorly poorly RB cord-329553-93tbgn2b 17 33 understood understand VBN cord-329553-93tbgn2b 17 34 , , , cord-329553-93tbgn2b 17 35 and and CC cord-329553-93tbgn2b 17 36 very very RB cord-329553-93tbgn2b 17 37 little little JJ cord-329553-93tbgn2b 17 38 is be VBZ cord-329553-93tbgn2b 17 39 known know VBN cord-329553-93tbgn2b 17 40 about about IN cord-329553-93tbgn2b 17 41 the the DT cord-329553-93tbgn2b 17 42 signals signal NNS cord-329553-93tbgn2b 17 43 that that WDT cord-329553-93tbgn2b 17 44 mediate mediate VBP cord-329553-93tbgn2b 17 45 the the DT cord-329553-93tbgn2b 17 46 Golgi Golgi NNP cord-329553-93tbgn2b 17 47 localization localization NN cord-329553-93tbgn2b 17 48 of of IN cord-329553-93tbgn2b 17 49 its -PRON- PRP$ cord-329553-93tbgn2b 17 50 resident resident JJ cord-329553-93tbgn2b 17 51 proteins protein NNS cord-329553-93tbgn2b 17 52 . . . cord-329553-93tbgn2b 18 1 Previous previous JJ cord-329553-93tbgn2b 18 2 studies study NNS cord-329553-93tbgn2b 18 3 have have VBP cord-329553-93tbgn2b 18 4 shown show VBN cord-329553-93tbgn2b 18 5 that that IN cord-329553-93tbgn2b 18 6 a a DT cord-329553-93tbgn2b 18 7 catalytically catalytically RB cord-329553-93tbgn2b 18 8 active active JJ cord-329553-93tbgn2b 18 9 fragment fragment NN cord-329553-93tbgn2b 18 10 of of IN cord-329553-93tbgn2b 18 11 ST ST NNP cord-329553-93tbgn2b 18 12 is be VBZ cord-329553-93tbgn2b 18 13 secreted secrete VBN cord-329553-93tbgn2b 18 14 Jamieson Jamieson NNP cord-329553-93tbgn2b 18 15 , , , cord-329553-93tbgn2b 18 16 1988 1988 CD cord-329553-93tbgn2b 18 17 , , , cord-329553-93tbgn2b 18 18 1989 1989 CD cord-329553-93tbgn2b 18 19 ; ; : cord-329553-93tbgn2b 18 20 Paulson Paulson NNP cord-329553-93tbgn2b 18 21 and and CC cord-329553-93tbgn2b 18 22 Colley Colley NNP cord-329553-93tbgn2b 18 23 , , , cord-329553-93tbgn2b 18 24 1989 1989 CD cord-329553-93tbgn2b 18 25 ) ) -RRB- cord-329553-93tbgn2b 18 26 . . . cord-329553-93tbgn2b 19 1 Further further JJ cord-329553-93tbgn2b 19 2 experiments experiment NNS cord-329553-93tbgn2b 19 3 also also RB cord-329553-93tbgn2b 19 4 showed show VBD cord-329553-93tbgn2b 19 5 that that IN cord-329553-93tbgn2b 19 6 a a DT cord-329553-93tbgn2b 19 7 41 41 CD cord-329553-93tbgn2b 19 8 - - HYPH cord-329553-93tbgn2b 19 9 -43 -43 NN cord-329553-93tbgn2b 19 10 kD kd JJ cord-329553-93tbgn2b 19 11 fragment fragment NN cord-329553-93tbgn2b 19 12 of of IN cord-329553-93tbgn2b 19 13 ST ST NNP cord-329553-93tbgn2b 19 14 could could MD cord-329553-93tbgn2b 19 15 be be VB cord-329553-93tbgn2b 19 16 released release VBN cord-329553-93tbgn2b 19 17 from from IN cord-329553-93tbgn2b 19 18 Golgi Golgi NNP cord-329553-93tbgn2b 19 19 membranes membrane NNS cord-329553-93tbgn2b 19 20 by by IN cord-329553-93tbgn2b 19 21 endogenous endogenous JJ cord-329553-93tbgn2b 19 22 cathepsin cathepsin NN cord-329553-93tbgn2b 19 23 D d NN cord-329553-93tbgn2b 19 24 - - HYPH cord-329553-93tbgn2b 19 25 like like JJ cord-329553-93tbgn2b 19 26 protease protease NN cord-329553-93tbgn2b 19 27 ( ( -LRB- cord-329553-93tbgn2b 19 28 Lammers Lammers NNP cord-329553-93tbgn2b 19 29 and and CC cord-329553-93tbgn2b 19 30 Jamieson Jamieson NNP cord-329553-93tbgn2b 19 31 , , , cord-329553-93tbgn2b 19 32 1988 1988 CD cord-329553-93tbgn2b 19 33 ; ; : cord-329553-93tbgn2b 19 34 Weinstein Weinstein NNP cord-329553-93tbgn2b 19 35 et et NNP cord-329553-93tbgn2b 19 36 al al NNP cord-329553-93tbgn2b 19 37 . . NNP cord-329553-93tbgn2b 19 38 , , , cord-329553-93tbgn2b 19 39 1987 1987 CD cord-329553-93tbgn2b 19 40 ) ) -RRB- cord-329553-93tbgn2b 19 41 . . . cord-329553-93tbgn2b 20 1 This this DT cord-329553-93tbgn2b 20 2 41 41 CD cord-329553-93tbgn2b 20 3 - - SYM cord-329553-93tbgn2b 20 4 4341 4341 CD cord-329553-93tbgn2b 20 5 ) ) -RRB- cord-329553-93tbgn2b 20 6 fragment fragment NN cord-329553-93tbgn2b 20 7 is be VBZ cord-329553-93tbgn2b 20 8 not not RB cord-329553-93tbgn2b 20 9 anchored anchor VBN cord-329553-93tbgn2b 20 10 in in IN cord-329553-93tbgn2b 20 11 the the DT cord-329553-93tbgn2b 20 12 membrane membrane NN cord-329553-93tbgn2b 20 13 but but CC cord-329553-93tbgn2b 20 14 is be VBZ cord-329553-93tbgn2b 20 15 still still RB cord-329553-93tbgn2b 20 16 enzymatically enzymatically RB cord-329553-93tbgn2b 20 17 active active JJ cord-329553-93tbgn2b 20 18 . . . cord-329553-93tbgn2b 21 1 Protein protein NN cord-329553-93tbgn2b 21 2 sequencing sequencing NN cord-329553-93tbgn2b 21 3 analysis analysis NN cord-329553-93tbgn2b 21 4 has have VBZ cord-329553-93tbgn2b 21 5 established establish VBN cord-329553-93tbgn2b 21 6 that that IN cord-329553-93tbgn2b 21 7 this this DT cord-329553-93tbgn2b 21 8 fragment fragment NN cord-329553-93tbgn2b 21 9 is be VBZ cord-329553-93tbgn2b 21 10 released release VBN cord-329553-93tbgn2b 21 11 by by IN cord-329553-93tbgn2b 21 12 cleavage cleavage NN cord-329553-93tbgn2b 21 13 between between IN cord-329553-93tbgn2b 21 14 residues residue NNS cord-329553-93tbgn2b 21 15 62 62 CD cord-329553-93tbgn2b 21 16 and and CC cord-329553-93tbgn2b 21 17 63 63 CD cord-329553-93tbgn2b 22 1 ( ( -LRB- cord-329553-93tbgn2b 22 2 Weinstein Weinstein NNP cord-329553-93tbgn2b 22 3 et et NNP cord-329553-93tbgn2b 22 4 al al NNP cord-329553-93tbgn2b 22 5 . . NNP cord-329553-93tbgn2b 22 6 , , , cord-329553-93tbgn2b 22 7 1987 1987 CD cord-329553-93tbgn2b 22 8 ) ) -RRB- cord-329553-93tbgn2b 22 9 . . . cord-329553-93tbgn2b 23 1 It -PRON- PRP cord-329553-93tbgn2b 23 2 seems seem VBZ cord-329553-93tbgn2b 23 3 that that IN cord-329553-93tbgn2b 23 4 the the DT cord-329553-93tbgn2b 23 5 secreted secrete VBN cord-329553-93tbgn2b 23 6 form form NN cord-329553-93tbgn2b 23 7 of of IN cord-329553-93tbgn2b 23 8 ST ST NNP cord-329553-93tbgn2b 23 9 is be VBZ cord-329553-93tbgn2b 23 10 also also RB cord-329553-93tbgn2b 23 11 the the DT cord-329553-93tbgn2b 23 12 result result NN cord-329553-93tbgn2b 23 13 of of IN cord-329553-93tbgn2b 23 14 cleavage cleavage NN cord-329553-93tbgn2b 23 15 around around IN cord-329553-93tbgn2b 23 16 this this DT cord-329553-93tbgn2b 23 17 site site NN cord-329553-93tbgn2b 23 18 . . . cord-329553-93tbgn2b 24 1 When when WRB cord-329553-93tbgn2b 24 2 fused fuse VBN cord-329553-93tbgn2b 24 3 to to IN cord-329553-93tbgn2b 24 4 a a DT cord-329553-93tbgn2b 24 5 cleavable cleavable JJ cord-329553-93tbgn2b 24 6 signal signal NN cord-329553-93tbgn2b 24 7 sequence sequence NN cord-329553-93tbgn2b 24 8 , , , cord-329553-93tbgn2b 24 9 an an DT cord-329553-93tbgn2b 24 10 enzymatically enzymatically RB cord-329553-93tbgn2b 24 11 active active JJ cord-329553-93tbgn2b 24 12 ST ST NNP cord-329553-93tbgn2b 24 13 fragment fragment NN cord-329553-93tbgn2b 24 14 from from IN cord-329553-93tbgn2b 24 15 residue residue NN cord-329553-93tbgn2b 24 16 58 58 CD cord-329553-93tbgn2b 24 17 to to IN cord-329553-93tbgn2b 24 18 the the DT cord-329553-93tbgn2b 24 19 COOH COOH NNP cord-329553-93tbgn2b 24 20 terminus terminus NN cord-329553-93tbgn2b 24 21 is be VBZ cord-329553-93tbgn2b 24 22 secreted secrete VBN cord-329553-93tbgn2b 24 23 in in IN cord-329553-93tbgn2b 24 24 transfected transfecte VBN cord-329553-93tbgn2b 24 25 cells cell NNS cord-329553-93tbgn2b 24 26 . . . cord-329553-93tbgn2b 25 1 These these DT cord-329553-93tbgn2b 25 2 observations observation NNS cord-329553-93tbgn2b 25 3 suggest suggest VBP cord-329553-93tbgn2b 25 4 that that IN cord-329553-93tbgn2b 25 5 the the DT cord-329553-93tbgn2b 25 6 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 25 7 region region NN cord-329553-93tbgn2b 25 8 of of IN cord-329553-93tbgn2b 25 9 ST ST NNP cord-329553-93tbgn2b 25 10 is be VBZ cord-329553-93tbgn2b 25 11 necessary necessary JJ cord-329553-93tbgn2b 25 12 for for IN cord-329553-93tbgn2b 25 13 membrane membrane NN cord-329553-93tbgn2b 25 14 anchorage anchorage NNP cord-329553-93tbgn2b 25 15 and and CC cord-329553-93tbgn2b 25 16 Golgi Golgi NNP cord-329553-93tbgn2b 25 17 localization localization NN cord-329553-93tbgn2b 25 18 Paulson Paulson NNP cord-329553-93tbgn2b 25 19 and and CC cord-329553-93tbgn2b 25 20 Colley Colley NNP cord-329553-93tbgn2b 25 21 , , , cord-329553-93tbgn2b 25 22 1989 1989 CD cord-329553-93tbgn2b 25 23 ) ) -RRB- cord-329553-93tbgn2b 25 24 . . . cord-329553-93tbgn2b 26 1 In in IN cord-329553-93tbgn2b 26 2 this this DT cord-329553-93tbgn2b 26 3 report report NN cord-329553-93tbgn2b 26 4 , , , cord-329553-93tbgn2b 26 5 we -PRON- PRP cord-329553-93tbgn2b 26 6 have have VBP cord-329553-93tbgn2b 26 7 fused fuse VBN cord-329553-93tbgn2b 26 8 different different JJ cord-329553-93tbgn2b 26 9 regions region NNS cord-329553-93tbgn2b 26 10 of of IN cord-329553-93tbgn2b 26 11 the the DT cord-329553-93tbgn2b 26 12 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 26 13 ST ST NNP cord-329553-93tbgn2b 26 14 sequence sequence NN cord-329553-93tbgn2b 26 15 to to IN cord-329553-93tbgn2b 26 16 the the DT cord-329553-93tbgn2b 26 17 ectodomain ectodomain NN cord-329553-93tbgn2b 26 18 of of IN cord-329553-93tbgn2b 26 19 dipeptidyl dipeptidyl JJ cord-329553-93tbgn2b 26 20 peptidase peptidase NN cord-329553-93tbgn2b 26 21 IV IV NNP cord-329553-93tbgn2b 26 22 ( ( -LRB- cord-329553-93tbgn2b 26 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 26 24 ) ) -RRB- cord-329553-93tbgn2b 26 25 , , , cord-329553-93tbgn2b 26 26 a a DT cord-329553-93tbgn2b 26 27 type type NN cord-329553-93tbgn2b 26 28 11 11 CD cord-329553-93tbgn2b 26 29 surface surface NN cord-329553-93tbgn2b 26 30 membrane membrane NN cord-329553-93tbgn2b 26 31 protein protein NN cord-329553-93tbgn2b 26 32 ( ( -LRB- cord-329553-93tbgn2b 26 33 Hong Hong NNP cord-329553-93tbgn2b 26 34 and and CC cord-329553-93tbgn2b 26 35 Doyle Doyle NNP cord-329553-93tbgn2b 26 36 , , , cord-329553-93tbgn2b 26 37 1990 1990 CD cord-329553-93tbgn2b 26 38 ; ; : cord-329553-93tbgn2b 26 39 Ogato Ogato NNP cord-329553-93tbgn2b 26 40 et et NNP cord-329553-93tbgn2b 26 41 al al NNP cord-329553-93tbgn2b 26 42 . . NNP cord-329553-93tbgn2b 26 43 , , , cord-329553-93tbgn2b 26 44 1989 1989 CD cord-329553-93tbgn2b 26 45 ) ) -RRB- cord-329553-93tbgn2b 26 46 to to TO cord-329553-93tbgn2b 26 47 study study VB cord-329553-93tbgn2b 26 48 their -PRON- PRP$ cord-329553-93tbgn2b 26 49 abilities ability NNS cord-329553-93tbgn2b 26 50 in in IN cord-329553-93tbgn2b 26 51 conferring confer VBG cord-329553-93tbgn2b 26 52 Golgi Golgi NNP cord-329553-93tbgn2b 26 53 localization localization NN cord-329553-93tbgn2b 26 54 ofthe ofthe DT cord-329553-93tbgn2b 26 55 chimeric chimeric JJ cord-329553-93tbgn2b 26 56 proteins protein NNS cord-329553-93tbgn2b 26 57 . . . cord-329553-93tbgn2b 27 1 We -PRON- PRP cord-329553-93tbgn2b 27 2 found find VBD cord-329553-93tbgn2b 27 3 that that IN cord-329553-93tbgn2b 27 4 the the DT cord-329553-93tbgn2b 27 5 17-residue 17-residue CD cord-329553-93tbgn2b 27 6 transmembrane transmembrane NN cord-329553-93tbgn2b 27 7 domain domain NN cord-329553-93tbgn2b 27 8 of of IN cord-329553-93tbgn2b 27 9 ST ST NNP cord-329553-93tbgn2b 27 10 is be VBZ cord-329553-93tbgn2b 27 11 sufficient sufficient JJ cord-329553-93tbgn2b 27 12 for for IN cord-329553-93tbgn2b 27 13 Golgi Golgi NNP cord-329553-93tbgn2b 27 14 localization localization NN cord-329553-93tbgn2b 27 15 and and CC cord-329553-93tbgn2b 27 16 that that IN cord-329553-93tbgn2b 27 17 this this DT cord-329553-93tbgn2b 27 18 se se FW cord-329553-93tbgn2b 27 19 - - HYPH cord-329553-93tbgn2b 27 20 Native Native NNP cord-329553-93tbgn2b 27 21 and and CC cord-329553-93tbgn2b 27 22 Chimeric chimeric JJ cord-329553-93tbgn2b 27 23 cDNA cdna NN cord-329553-93tbgn2b 27 24 DPPIV DPPIV NNP cord-329553-93tbgn2b 27 25 . . . cord-329553-93tbgn2b 28 1 A a DT cord-329553-93tbgn2b 28 2 3 3 CD cord-329553-93tbgn2b 28 3 .2-kbp .2-kbp . cord-329553-93tbgn2b 29 1 EcoRI EcoRI NNP cord-329553-93tbgn2b 29 2 cDNA cdna NN cord-329553-93tbgn2b 29 3 fragment fragment NN cord-329553-93tbgn2b 29 4 encoding encode VBG cord-329553-93tbgn2b 29 5 for for IN cord-329553-93tbgn2b 29 6 DPPIV DPPIV NNP cord-329553-93tbgn2b 29 7 in in IN cord-329553-93tbgn2b 29 8 the the DT cord-329553-93tbgn2b 29 9 pGEM-4Z pgem-4z CD cord-329553-93tbgn2b 29 10 vector vector NN cord-329553-93tbgn2b 29 11 ( ( -LRB- cord-329553-93tbgn2b 29 12 its -PRON- PRP$ cord-329553-93tbgn2b 29 13 5 5 CD cord-329553-93tbgn2b 29 14 ' ' NN cord-329553-93tbgn2b 29 15 end end NN cord-329553-93tbgn2b 29 16 facing face VBG cord-329553-93tbgn2b 29 17 the the DT cord-329553-93tbgn2b 29 18 Sp6 sp6 CD cord-329553-93tbgn2b 29 19 promoter promoter NN cord-329553-93tbgn2b 29 20 ) ) -RRB- cord-329553-93tbgn2b 29 21 has have VBZ cord-329553-93tbgn2b 29 22 been be VBN cord-329553-93tbgn2b 29 23 described describe VBN cord-329553-93tbgn2b 29 24 ( ( -LRB- cord-329553-93tbgn2b 29 25 Hong Hong NNP cord-329553-93tbgn2b 29 26 and and CC cord-329553-93tbgn2b 29 27 Doyle Doyle NNP cord-329553-93tbgn2b 29 28 , , , cord-329553-93tbgn2b 29 29 1988 1988 CD cord-329553-93tbgn2b 29 30 ) ) -RRB- cord-329553-93tbgn2b 29 31 . . . cord-329553-93tbgn2b 30 1 Tb Tb NNP cord-329553-93tbgn2b 30 2 facilitate facilitate VBP cord-329553-93tbgn2b 30 3 the the DT cord-329553-93tbgn2b 30 4 construction construction NN cord-329553-93tbgn2b 30 5 of of IN cord-329553-93tbgn2b 30 6 chimeric chimeric JJ cord-329553-93tbgn2b 30 7 cDNA cdna NN cord-329553-93tbgn2b 30 8 , , , cord-329553-93tbgn2b 30 9 the the DT cord-329553-93tbgn2b 30 10 recombinant recombinant JJ cord-329553-93tbgn2b 30 11 plasmid plasmid NN cord-329553-93tbgn2b 30 12 was be VBD cord-329553-93tbgn2b 30 13 digested digest VBN cord-329553-93tbgn2b 30 14 with with IN cord-329553-93tbgn2b 30 15 the the DT cord-329553-93tbgn2b 30 16 restriction restriction NN cord-329553-93tbgn2b 30 17 enzymes enzyme NNS cord-329553-93tbgn2b 30 18 XhoI XhoI NNP cord-329553-93tbgn2b 30 19 and and CC cord-329553-93tbgn2b 30 20 BamHI BamHI NNP cord-329553-93tbgn2b 30 21 , , , cord-329553-93tbgn2b 30 22 blunt blunt JJ cord-329553-93tbgn2b 30 23 ended end VBD cord-329553-93tbgn2b 30 24 , , , cord-329553-93tbgn2b 30 25 and and CC cord-329553-93tbgn2b 30 26 then then RB cord-329553-93tbgn2b 30 27 self self NN cord-329553-93tbgn2b 30 28 - - HYPH cord-329553-93tbgn2b 30 29 ligated ligate VBN cord-329553-93tbgn2b 30 30 . . . cord-329553-93tbgn2b 31 1 This this DT cord-329553-93tbgn2b 31 2 resulted result VBD cord-329553-93tbgn2b 31 3 in in IN cord-329553-93tbgn2b 31 4 the the DT cord-329553-93tbgn2b 31 5 deletion deletion NN cord-329553-93tbgn2b 31 6 of of IN cord-329553-93tbgn2b 31 7 most most JJS cord-329553-93tbgn2b 31 8 of of IN cord-329553-93tbgn2b 31 9 the the DT cord-329553-93tbgn2b 31 10 3'-untranslated 3'-untranslated JJ cord-329553-93tbgn2b 31 11 region region NN cord-329553-93tbgn2b 31 12 of of IN cord-329553-93tbgn2b 31 13 DPPIV DPPIV NNP cord-329553-93tbgn2b 31 14 cDNA cdna NN cord-329553-93tbgn2b 31 15 . . . cord-329553-93tbgn2b 32 1 The the DT cord-329553-93tbgn2b 32 2 resulting result VBG cord-329553-93tbgn2b 32 3 construct construct NN cord-329553-93tbgn2b 32 4 was be VBD cord-329553-93tbgn2b 32 5 referred refer VBN cord-329553-93tbgn2b 32 6 to to IN cord-329553-93tbgn2b 32 7 as as RB cord-329553-93tbgn2b 32 8 pGEM pGEM NNP cord-329553-93tbgn2b 32 9 / / SYM cord-329553-93tbgn2b 32 10 D4S d4s NN cord-329553-93tbgn2b 32 11 ) ) -RRB- cord-329553-93tbgn2b 32 12 . . . cord-329553-93tbgn2b 33 1 This this DT cord-329553-93tbgn2b 33 2 process process NN cord-329553-93tbgn2b 33 3 resulted result VBD cord-329553-93tbgn2b 33 4 in in IN cord-329553-93tbgn2b 33 5 the the DT cord-329553-93tbgn2b 33 6 creation creation NN cord-329553-93tbgn2b 33 7 of of IN cord-329553-93tbgn2b 33 8 a a DT cord-329553-93tbgn2b 33 9 unique unique JJ cord-329553-93tbgn2b 33 10 EcoRl ecorl NN cord-329553-93tbgn2b 33 11 site site NN cord-329553-93tbgn2b 33 12 at at IN cord-329553-93tbgn2b 33 13 the the DT cord-329553-93tbgn2b 33 14 5 5 CD cord-329553-93tbgn2b 33 15 ' ' NN cord-329553-93tbgn2b 33 16 end end NN cord-329553-93tbgn2b 33 17 and and CC cord-329553-93tbgn2b 33 18 unique unique JJ cord-329553-93tbgn2b 33 19 Xhol Xhol NNP cord-329553-93tbgn2b 33 20 , , , cord-329553-93tbgn2b 33 21 XbaI XbaI NNP cord-329553-93tbgn2b 33 22 , , , cord-329553-93tbgn2b 33 23 Sall Sall NNP cord-329553-93tbgn2b 33 24 , , , cord-329553-93tbgn2b 33 25 and and CC cord-329553-93tbgn2b 33 26 HindIII HindIII NNP cord-329553-93tbgn2b 33 27 sites site NNS cord-329553-93tbgn2b 33 28 at at IN cord-329553-93tbgn2b 33 29 the the DT cord-329553-93tbgn2b 33 30 3 3 CD cord-329553-93tbgn2b 33 31 ' ' NN cord-329553-93tbgn2b 33 32 end end NN cord-329553-93tbgn2b 33 33 of of IN cord-329553-93tbgn2b 33 34 the the DT cord-329553-93tbgn2b 33 35 DPPIV DPPIV NNP cord-329553-93tbgn2b 33 36 coding coding NN cord-329553-93tbgn2b 33 37 region region NN cord-329553-93tbgn2b 33 38 . . . cord-329553-93tbgn2b 34 1 There there EX cord-329553-93tbgn2b 34 2 is be VBZ cord-329553-93tbgn2b 34 3 a a DT cord-329553-93tbgn2b 34 4 unique unique JJ cord-329553-93tbgn2b 34 5 Nod nod JJ cord-329553-93tbgn2b 34 6 site site NN cord-329553-93tbgn2b 34 7 in in IN cord-329553-93tbgn2b 34 8 the the DT cord-329553-93tbgn2b 34 9 codons codon NNS cord-329553-93tbgn2b 34 10 for for IN cord-329553-93tbgn2b 34 11 residues residue NNS cord-329553-93tbgn2b 34 12 33 33 CD cord-329553-93tbgn2b 34 13 - - SYM cord-329553-93tbgn2b 34 14 35 35 CD cord-329553-93tbgn2b 34 15 in in IN cord-329553-93tbgn2b 34 16 the the DT cord-329553-93tbgn2b 34 17 DPPIV DPPIV NNP cord-329553-93tbgn2b 34 18 cDNA cdna NN cord-329553-93tbgn2b 34 19 . . . cord-329553-93tbgn2b 35 1 The the DT cord-329553-93tbgn2b 35 2 5 5 CD cord-329553-93tbgn2b 35 3 ' ' `` cord-329553-93tbgn2b 35 4 unique unique JJ cord-329553-93tbgn2b 35 5 EcoRl ecorl NN cord-329553-93tbgn2b 35 6 site site NN cord-329553-93tbgn2b 35 7 and and CC cord-329553-93tbgn2b 35 8 this this DT cord-329553-93tbgn2b 35 9 Nod Nod NNP cord-329553-93tbgn2b 35 10 site site NN cord-329553-93tbgn2b 35 11 were be VBD cord-329553-93tbgn2b 35 12 used use VBN cord-329553-93tbgn2b 35 13 to to TO cord-329553-93tbgn2b 35 14 replace replace VB cord-329553-93tbgn2b 35 15 the the DT cord-329553-93tbgn2b 35 16 DNA dna NN cord-329553-93tbgn2b 35 17 sequence sequence NN cord-329553-93tbgn2b 35 18 encoding encode VBG cord-329553-93tbgn2b 35 19 for for IN cord-329553-93tbgn2b 35 20 the the DT cord-329553-93tbgn2b 35 21 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 35 22 32-residue 32-residue CD cord-329553-93tbgn2b 35 23 sequence sequence NN cord-329553-93tbgn2b 35 24 of of IN cord-329553-93tbgn2b 35 25 DPPIV DPPIV NNP cord-329553-93tbgn2b 35 26 with with IN cord-329553-93tbgn2b 35 27 various various JJ cord-329553-93tbgn2b 35 28 DNA dna NN cord-329553-93tbgn2b 35 29 sequences sequence NNS cord-329553-93tbgn2b 35 30 encoding encode VBG cord-329553-93tbgn2b 35 31 for for IN cord-329553-93tbgn2b 35 32 different different JJ cord-329553-93tbgn2b 35 33 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 35 34 ST ST NNP cord-329553-93tbgn2b 35 35 sequences sequence NNS cord-329553-93tbgn2b 35 36 . . . cord-329553-93tbgn2b 36 1 ST ST NNP cord-329553-93tbgn2b 36 2 A A NNP cord-329553-93tbgn2b 36 3 cDNA cdna NN cord-329553-93tbgn2b 36 4 clone clone NN cord-329553-93tbgn2b 36 5 of of IN cord-329553-93tbgn2b 36 6 unknown unknown JJ cord-329553-93tbgn2b 36 7 identity identity NN cord-329553-93tbgn2b 36 8 was be VBD cord-329553-93tbgn2b 36 9 obtained obtain VBN cord-329553-93tbgn2b 36 10 by by IN cord-329553-93tbgn2b 36 11 screening screen VBG cord-329553-93tbgn2b 36 12 a a DT cord-329553-93tbgn2b 36 13 rat rat NN cord-329553-93tbgn2b 36 14 kidney kidney NN cord-329553-93tbgn2b 36 15 kgtll kgtll NNP cord-329553-93tbgn2b 36 16 cDNA cDNA NNP cord-329553-93tbgn2b 36 17 library library NNP cord-329553-93tbgn2b 36 18 with with IN cord-329553-93tbgn2b 36 19 polyclonal polyclonal JJ cord-329553-93tbgn2b 36 20 antibodies antibody NNS cord-329553-93tbgn2b 36 21 against against IN cord-329553-93tbgn2b 36 22 rat rat NN cord-329553-93tbgn2b 36 23 Golgi Golgi NNP cord-329553-93tbgn2b 36 24 WGAbinding WGAbinding NNP cord-329553-93tbgn2b 36 25 proteins protein NNS cord-329553-93tbgn2b 36 26 . . . cord-329553-93tbgn2b 37 1 Limited limited JJ cord-329553-93tbgn2b 37 2 sequence sequence NN cord-329553-93tbgn2b 37 3 analysis analysis NN cord-329553-93tbgn2b 37 4 established establish VBD cord-329553-93tbgn2b 37 5 that that IN cord-329553-93tbgn2b 37 6 it -PRON- PRP cord-329553-93tbgn2b 37 7 encodes encode VBZ cord-329553-93tbgn2b 37 8 for for IN cord-329553-93tbgn2b 37 9 ST ST NNP cord-329553-93tbgn2b 37 10 . . . cord-329553-93tbgn2b 38 1 To to TO cord-329553-93tbgn2b 38 2 facilitate facilitate VB cord-329553-93tbgn2b 38 3 the the DT cord-329553-93tbgn2b 38 4 analysis analysis NN cord-329553-93tbgn2b 38 5 , , , cord-329553-93tbgn2b 38 6 oligonucleotides oligonucleotide NNS cord-329553-93tbgn2b 38 7 1 1 CD cord-329553-93tbgn2b 38 8 and and CC cord-329553-93tbgn2b 38 9 2 2 CD cord-329553-93tbgn2b 38 10 were be VBD cord-329553-93tbgn2b 38 11 used use VBN cord-329553-93tbgn2b 38 12 to to TO cord-329553-93tbgn2b 38 13 retrieve retrieve VB cord-329553-93tbgn2b 38 14 the the DT cord-329553-93tbgn2b 38 15 ST ST NNP cord-329553-93tbgn2b 38 16 coding coding NN cord-329553-93tbgn2b 38 17 region region NN cord-329553-93tbgn2b 38 18 by by IN cord-329553-93tbgn2b 38 19 polymerase polymerase NN cord-329553-93tbgn2b 38 20 chain chain NN cord-329553-93tbgn2b 38 21 reaction reaction NN cord-329553-93tbgn2b 38 22 ( ( -LRB- cord-329553-93tbgn2b 38 23 PCR PCR NNP cord-329553-93tbgn2b 38 24 ) ) -RRB- cord-329553-93tbgn2b 38 25 . . . cord-329553-93tbgn2b 39 1 The the DT cord-329553-93tbgn2b 39 2 PCR PCR NNP cord-329553-93tbgn2b 39 3 product product NN cord-329553-93tbgn2b 39 4 was be VBD cord-329553-93tbgn2b 39 5 digested digest VBN cord-329553-93tbgn2b 39 6 with with IN cord-329553-93tbgn2b 39 7 Xhol Xhol NNP cord-329553-93tbgn2b 39 8 and and CC cord-329553-93tbgn2b 39 9 inserted insert VBD cord-329553-93tbgn2b 39 10 into into IN cord-329553-93tbgn2b 39 11 the the DT cord-329553-93tbgn2b 39 12 Xhol Xhol NNP cord-329553-93tbgn2b 39 13 site site NN cord-329553-93tbgn2b 39 14 of of IN cord-329553-93tbgn2b 39 15 pGEM-11Z(+ pGEM-11Z(+ NNP cord-329553-93tbgn2b 39 16 ) ) -RRB- cord-329553-93tbgn2b 39 17 with with IN cord-329553-93tbgn2b 39 18 its -PRON- PRP$ cord-329553-93tbgn2b 39 19 5 5 CD cord-329553-93tbgn2b 39 20 ' ' NN cord-329553-93tbgn2b 39 21 end end NN cord-329553-93tbgn2b 39 22 facing face VBG cord-329553-93tbgn2b 39 23 the the DT cord-329553-93tbgn2b 39 24 Sp6 sp6 CD cord-329553-93tbgn2b 39 25 promoter promoter NN cord-329553-93tbgn2b 39 26 . . . cord-329553-93tbgn2b 40 1 S64D. s64d. VB cord-329553-93tbgn2b 41 1 Oligonucleotides oligonucleotides NN cord-329553-93tbgn2b 41 2 1 1 CD cord-329553-93tbgn2b 41 3 and and CC cord-329553-93tbgn2b 41 4 3 3 CD cord-329553-93tbgn2b 41 5 were be VBD cord-329553-93tbgn2b 41 6 used use VBN cord-329553-93tbgn2b 41 7 to to TO cord-329553-93tbgn2b 41 8 retrieve retrieve VB cord-329553-93tbgn2b 41 9 ST ST NNP cord-329553-93tbgn2b 41 10 cDNA cdna NN cord-329553-93tbgn2b 41 11 encoding encoding NN cord-329553-93tbgn2b 41 12 for for IN cord-329553-93tbgn2b 41 13 the the DT cord-329553-93tbgn2b 41 14 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 41 15 64-residue 64-residue CD cord-329553-93tbgn2b 41 16 sequence sequence NN cord-329553-93tbgn2b 41 17 by by IN cord-329553-93tbgn2b 41 18 PCR PCR NNP cord-329553-93tbgn2b 41 19 . . . cord-329553-93tbgn2b 42 1 The the DT cord-329553-93tbgn2b 42 2 PCR PCR NNP cord-329553-93tbgn2b 42 3 product product NN cord-329553-93tbgn2b 42 4 was be VBD cord-329553-93tbgn2b 42 5 digested digest VBN cord-329553-93tbgn2b 42 6 with with IN cord-329553-93tbgn2b 42 7 EcoRI EcoRI NNP cord-329553-93tbgn2b 42 8 and and CC cord-329553-93tbgn2b 42 9 Nod Nod NNP cord-329553-93tbgn2b 42 10 and and CC cord-329553-93tbgn2b 42 11 used use VBD cord-329553-93tbgn2b 42 12 to to TO cord-329553-93tbgn2b 42 13 replace replace VB cord-329553-93tbgn2b 42 14 the the DT cord-329553-93tbgn2b 42 15 5 5 CD cord-329553-93tbgn2b 42 16 ' ' '' cord-329553-93tbgn2b 42 17 EcoRI EcoRI NNP cord-329553-93tbgn2b 42 18 - - HYPH cord-329553-93tbgn2b 42 19 NotI NotI NNP cord-329553-93tbgn2b 42 20 sequence sequence NN cord-329553-93tbgn2b 42 21 of of IN cord-329553-93tbgn2b 42 22 DPPIV DPPIV NNP cord-329553-93tbgn2b 42 23 in in IN cord-329553-93tbgn2b 42 24 pGEM pGEM NNS cord-329553-93tbgn2b 42 25 / / SYM cord-329553-93tbgn2b 42 26 D4S. d4s. NN cord-329553-93tbgn2b 43 1 The the DT cord-329553-93tbgn2b 43 2 resulting result VBG cord-329553-93tbgn2b 43 3 chimeric chimeric JJ cord-329553-93tbgn2b 43 4 cDNA cdna NN cord-329553-93tbgn2b 43 5 thus thus RB cord-329553-93tbgn2b 43 6 encodes encode VBZ cord-329553-93tbgn2b 43 7 for for IN cord-329553-93tbgn2b 43 8 the the DT cord-329553-93tbgn2b 43 9 fusion fusion NN cord-329553-93tbgn2b 43 10 protein protein NN cord-329553-93tbgn2b 43 11 S64D S64D NNP cord-329553-93tbgn2b 43 12 . . . cord-329553-93tbgn2b 44 1 S51D. S51D. : cord-329553-93tbgn2b 44 2 Oligonucleotides Oligonucleotides NNP cord-329553-93tbgn2b 44 3 1 1 CD cord-329553-93tbgn2b 44 4 and and CC cord-329553-93tbgn2b 44 5 4 4 CD cord-329553-93tbgn2b 44 6 were be VBD cord-329553-93tbgn2b 44 7 used use VBN cord-329553-93tbgn2b 44 8 to to TO cord-329553-93tbgn2b 44 9 create create VB cord-329553-93tbgn2b 44 10 chimeric chimeric JJ cord-329553-93tbgn2b 44 11 cDNA cdna NN cord-329553-93tbgn2b 44 12 for for IN cord-329553-93tbgn2b 44 13 fusion fusion NN cord-329553-93tbgn2b 44 14 protein protein NN cord-329553-93tbgn2b 44 15 S51D S51D NNP cord-329553-93tbgn2b 44 16 as as IN cord-329553-93tbgn2b 44 17 described describe VBN cord-329553-93tbgn2b 44 18 above above RB cord-329553-93tbgn2b 44 19 for for IN cord-329553-93tbgn2b 44 20 S64D. s64d. ADD cord-329553-93tbgn2b 45 1 The the DT cord-329553-93tbgn2b 45 2 Journal Journal NNP cord-329553-93tbgn2b 45 3 .of .of . cord-329553-93tbgn2b 46 1 Cell cell NN cord-329553-93tbgn2b 46 2 Biology biology NN cord-329553-93tbgn2b 46 3 , , , cord-329553-93tbgn2b 46 4 Volume volume NN cord-329553-93tbgn2b 46 5 117 117 CD cord-329553-93tbgn2b 46 6 , , , cord-329553-93tbgn2b 46 7 1992 1992 CD cord-329553-93tbgn2b 47 1 S46D. S46D. NNP cord-329553-93tbgn2b 47 2 Chimeric chimeric JJ cord-329553-93tbgn2b 47 3 cDNA cdna NN cord-329553-93tbgn2b 47 4 coding code VBG cord-329553-93tbgn2b 47 5 for for IN cord-329553-93tbgn2b 47 6 S46D S46D NNP cord-329553-93tbgn2b 47 7 was be VBD cord-329553-93tbgn2b 47 8 similarly similarly RB cord-329553-93tbgn2b 47 9 constructed construct VBN cord-329553-93tbgn2b 47 10 by by IN cord-329553-93tbgn2b 47 11 using use VBG cord-329553-93tbgn2b 47 12 oligonucleotides oligonucleotide NNS cord-329553-93tbgn2b 47 13 1 1 CD cord-329553-93tbgn2b 47 14 and and CC cord-329553-93tbgn2b 47 15 5 5 CD cord-329553-93tbgn2b 47 16 . . . cord-329553-93tbgn2b 48 1 S40D. S40D. . cord-329553-93tbgn2b 49 1 This this DT cord-329553-93tbgn2b 49 2 was be VBD cord-329553-93tbgn2b 49 3 constructed construct VBN cord-329553-93tbgn2b 49 4 as as IN cord-329553-93tbgn2b 49 5 above above IN cord-329553-93tbgn2b 49 6 using use VBG cord-329553-93tbgn2b 49 7 oligonucleotides oligonucleotide NNS cord-329553-93tbgn2b 49 8 1 1 CD cord-329553-93tbgn2b 49 9 and and CC cord-329553-93tbgn2b 49 10 6 6 CD cord-329553-93tbgn2b 49 11 . . . cord-329553-93tbgn2b 50 1 S33D. S33D. NNPS cord-329553-93tbgn2b 51 1 Oligonucleotides oligonucleotides NN cord-329553-93tbgn2b 51 2 1 1 CD cord-329553-93tbgn2b 51 3 and and CC cord-329553-93tbgn2b 51 4 7 7 CD cord-329553-93tbgn2b 51 5 were be VBD cord-329553-93tbgn2b 51 6 used use VBN cord-329553-93tbgn2b 51 7 to to TO cord-329553-93tbgn2b 51 8 construct construct VB cord-329553-93tbgn2b 51 9 chimeric chimeric JJ cord-329553-93tbgn2b 51 10 cDNA cdna NN cord-329553-93tbgn2b 51 11 for for IN cord-329553-93tbgn2b 51 12 S33D s33d PDT cord-329553-93tbgn2b 51 13 the the DT cord-329553-93tbgn2b 51 14 same same JJ cord-329553-93tbgn2b 51 15 way way NN cord-329553-93tbgn2b 51 16 as as IN cord-329553-93tbgn2b 51 17 described describe VBN cord-329553-93tbgn2b 51 18 above above RB cord-329553-93tbgn2b 51 19 for for IN cord-329553-93tbgn2b 51 20 S64D. s64d. ADD cord-329553-93tbgn2b 51 21 S27D. S27D. NNS cord-329553-93tbgn2b 52 1 This this DT cord-329553-93tbgn2b 52 2 was be VBD cord-329553-93tbgn2b 52 3 created create VBN cord-329553-93tbgn2b 52 4 as as IN cord-329553-93tbgn2b 52 5 above above IN cord-329553-93tbgn2b 52 6 using use VBG cord-329553-93tbgn2b 52 7 oligonucleotides oligonucleotide NNS cord-329553-93tbgn2b 52 8 1 1 CD cord-329553-93tbgn2b 52 9 and and CC cord-329553-93tbgn2b 52 10 8 8 CD cord-329553-93tbgn2b 52 11 . . . cord-329553-93tbgn2b 53 1 S26D. S26D. NNP cord-329553-93tbgn2b 54 1 Oligonucleotides oligonucleotides NN cord-329553-93tbgn2b 54 2 1 1 CD cord-329553-93tbgn2b 54 3 and and CC cord-329553-93tbgn2b 54 4 9 9 CD cord-329553-93tbgn2b 54 5 were be VBD cord-329553-93tbgn2b 54 6 used use VBN cord-329553-93tbgn2b 54 7 to to TO cord-329553-93tbgn2b 54 8 construct construct VB cord-329553-93tbgn2b 54 9 the the DT cord-329553-93tbgn2b 54 10 chimeric chimeric JJ cord-329553-93tbgn2b 54 11 cDNA cdna NN cord-329553-93tbgn2b 54 12 for for IN cord-329553-93tbgn2b 54 13 S26D. S26D. NNP cord-329553-93tbgn2b 54 14 S9D. S9D. NNP cord-329553-93tbgn2b 54 15 Oligonucleotides Oligonucleotides NNP cord-329553-93tbgn2b 54 16 10 10 CD cord-329553-93tbgn2b 54 17 and and CC cord-329553-93tbgn2b 54 18 11 11 CD cord-329553-93tbgn2b 54 19 were be VBD cord-329553-93tbgn2b 54 20 used use VBN cord-329553-93tbgn2b 54 21 to to TO cord-329553-93tbgn2b 54 22 modify modify VB cord-329553-93tbgn2b 54 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 54 24 cDNA cdna NN cord-329553-93tbgn2b 54 25 by by IN cord-329553-93tbgn2b 54 26 PCR PCR NNP cord-329553-93tbgn2b 54 27 ( ( -LRB- cord-329553-93tbgn2b 54 28 DPPIV DPPIV NNP cord-329553-93tbgn2b 54 29 cDNA cdna NN cord-329553-93tbgn2b 54 30 as as IN cord-329553-93tbgn2b 54 31 template template NN cord-329553-93tbgn2b 54 32 ) ) -RRB- cord-329553-93tbgn2b 54 33 . . . cord-329553-93tbgn2b 55 1 The the DT cord-329553-93tbgn2b 55 2 PCR PCR NNP cord-329553-93tbgn2b 55 3 product product NN cord-329553-93tbgn2b 55 4 was be VBD cord-329553-93tbgn2b 55 5 digested digest VBN cord-329553-93tbgn2b 55 6 with with IN cord-329553-93tbgn2b 55 7 XhoI XhoI NNP cord-329553-93tbgn2b 55 8 and and CC cord-329553-93tbgn2b 55 9 inserted insert VBD cord-329553-93tbgn2b 55 10 into into IN cord-329553-93tbgn2b 55 11 the the DT cord-329553-93tbgn2b 55 12 Xhol Xhol NNP cord-329553-93tbgn2b 55 13 site site NN cord-329553-93tbgn2b 55 14 of of IN cord-329553-93tbgn2b 55 15 pGEM-11Z(+ pGEM-11Z(+ NNP cord-329553-93tbgn2b 55 16 ) ) -RRB- cord-329553-93tbgn2b 55 17 . . . cord-329553-93tbgn2b 56 1 This this DT cord-329553-93tbgn2b 56 2 process process NN cord-329553-93tbgn2b 56 3 resulted result VBD cord-329553-93tbgn2b 56 4 in in IN cord-329553-93tbgn2b 56 5 the the DT cord-329553-93tbgn2b 56 6 substitution substitution NN cord-329553-93tbgn2b 56 7 of of IN cord-329553-93tbgn2b 56 8 DPPIV DPPIV NNP cord-329553-93tbgn2b 56 9 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 56 10 domain domain NN cord-329553-93tbgn2b 56 11 by by IN cord-329553-93tbgn2b 56 12 that that DT cord-329553-93tbgn2b 56 13 of of IN cord-329553-93tbgn2b 56 14 ST ST NNP cord-329553-93tbgn2b 56 15 . . . cord-329553-93tbgn2b 57 1 S10 S10 NNP cord-329553-93tbgn2b 57 2 - - HYPH cord-329553-93tbgn2b 57 3 33D. 33d. CD cord-329553-93tbgn2b 57 4 Oligonucleotides Oligonucleotides NNP cord-329553-93tbgn2b 57 5 7 7 CD cord-329553-93tbgn2b 57 6 and and CC cord-329553-93tbgn2b 57 7 9 9 CD cord-329553-93tbgn2b 57 8 were be VBD cord-329553-93tbgn2b 57 9 used use VBN cord-329553-93tbgn2b 57 10 by by IN cord-329553-93tbgn2b 57 11 PCR PCR NNP cord-329553-93tbgn2b 57 12 ( ( -LRB- cord-329553-93tbgn2b 57 13 ST ST NNP cord-329553-93tbgn2b 57 14 cDNA cdna NN cord-329553-93tbgn2b 57 15 as as IN cord-329553-93tbgn2b 57 16 template template NN cord-329553-93tbgn2b 57 17 ) ) -RRB- cord-329553-93tbgn2b 57 18 to to TO cord-329553-93tbgn2b 57 19 create create VB cord-329553-93tbgn2b 57 20 a a DT cord-329553-93tbgn2b 57 21 cDNA cdna NN cord-329553-93tbgn2b 57 22 fragment fragment NN cord-329553-93tbgn2b 57 23 that that WDT cord-329553-93tbgn2b 57 24 encodes encode VBZ cord-329553-93tbgn2b 57 25 a a DT cord-329553-93tbgn2b 57 26 protein protein NN cord-329553-93tbgn2b 57 27 fragment fragment NN cord-329553-93tbgn2b 57 28 in in IN cord-329553-93tbgn2b 57 29 which which WDT cord-329553-93tbgn2b 57 30 the the DT cord-329553-93tbgn2b 57 31 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 57 32 6-residue 6-residue CD cord-329553-93tbgn2b 57 33 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 57 34 domain domain NN cord-329553-93tbgn2b 57 35 of of IN cord-329553-93tbgn2b 57 36 DPPIV DPPIV NNP cord-329553-93tbgn2b 57 37 was be VBD cord-329553-93tbgn2b 57 38 fused fuse VBN cord-329553-93tbgn2b 57 39 to to IN cord-329553-93tbgn2b 57 40 an an DT cord-329553-93tbgn2b 57 41 ST ST NNP cord-329553-93tbgn2b 57 42 sequence sequence NN cord-329553-93tbgn2b 57 43 from from IN cord-329553-93tbgn2b 57 44 residue residue NN cord-329553-93tbgn2b 57 45 10 10 CD cord-329553-93tbgn2b 57 46 to to IN cord-329553-93tbgn2b 57 47 33 33 CD cord-329553-93tbgn2b 57 48 . . . cord-329553-93tbgn2b 58 1 The the DT cord-329553-93tbgn2b 58 2 resulting result VBG cord-329553-93tbgn2b 58 3 PCR pcr NN cord-329553-93tbgn2b 58 4 fragment fragment NN cord-329553-93tbgn2b 58 5 was be VBD cord-329553-93tbgn2b 58 6 digested digest VBN cord-329553-93tbgn2b 58 7 with with IN cord-329553-93tbgn2b 58 8 EcoRl EcoRl NNP cord-329553-93tbgn2b 58 9 and and CC cord-329553-93tbgn2b 58 10 Nod Nod NNP cord-329553-93tbgn2b 58 11 and and CC cord-329553-93tbgn2b 58 12 used use VBD cord-329553-93tbgn2b 58 13 to to TO cord-329553-93tbgn2b 58 14 replace replace VB cord-329553-93tbgn2b 58 15 the the DT cord-329553-93tbgn2b 58 16 5 5 CD cord-329553-93tbgn2b 58 17 ' ' '' cord-329553-93tbgn2b 58 18 EcoRI EcoRI NNP cord-329553-93tbgn2b 58 19 - - HYPH cord-329553-93tbgn2b 58 20 NotI NotI NNP cord-329553-93tbgn2b 58 21 region region NN cord-329553-93tbgn2b 58 22 of of IN cord-329553-93tbgn2b 58 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 58 24 cDNA cdna NN cord-329553-93tbgn2b 58 25 . . . cord-329553-93tbgn2b 59 1 S10 S10 NNP cord-329553-93tbgn2b 59 2 - - HYPH cord-329553-93tbgn2b 59 3 26D. 26D. NNP cord-329553-93tbgn2b 60 1 Oligonucleotides Oligonucleotides NNP cord-329553-93tbgn2b 60 2 9 9 CD cord-329553-93tbgn2b 60 3 and and CC cord-329553-93tbgn2b 60 4 12 12 CD cord-329553-93tbgn2b 60 5 were be VBD cord-329553-93tbgn2b 60 6 used use VBN cord-329553-93tbgn2b 60 7 by by IN cord-329553-93tbgn2b 60 8 PCR PCR NNP cord-329553-93tbgn2b 60 9 ( ( -LRB- cord-329553-93tbgn2b 60 10 ST ST NNP cord-329553-93tbgn2b 60 11 cDNA cdna NN cord-329553-93tbgn2b 60 12 as as IN cord-329553-93tbgn2b 60 13 template template NN cord-329553-93tbgn2b 60 14 ) ) -RRB- cord-329553-93tbgn2b 60 15 to to TO cord-329553-93tbgn2b 60 16 create create VB cord-329553-93tbgn2b 60 17 a a DT cord-329553-93tbgn2b 60 18 cDNA cdna NN cord-329553-93tbgn2b 60 19 fragment fragment NN cord-329553-93tbgn2b 60 20 . . . cord-329553-93tbgn2b 61 1 After after IN cord-329553-93tbgn2b 61 2 digesting digest VBG cord-329553-93tbgn2b 61 3 with with IN cord-329553-93tbgn2b 61 4 EcoRl EcoRl NNP cord-329553-93tbgn2b 61 5 and and CC cord-329553-93tbgn2b 61 6 Nod Nod NNP cord-329553-93tbgn2b 61 7 , , , cord-329553-93tbgn2b 61 8 this this DT cord-329553-93tbgn2b 61 9 PCR PCR NNP cord-329553-93tbgn2b 61 10 fragment fragment NN cord-329553-93tbgn2b 61 11 was be VBD cord-329553-93tbgn2b 61 12 used use VBN cord-329553-93tbgn2b 61 13 to to TO cord-329553-93tbgn2b 61 14 replace replace VB cord-329553-93tbgn2b 61 15 the the DT cord-329553-93tbgn2b 61 16 EcoRI ecori NN cord-329553-93tbgn2b 61 17 - - HYPH cord-329553-93tbgn2b 61 18 Notl Notl NNP cord-329553-93tbgn2b 61 19 region region NN cord-329553-93tbgn2b 61 20 of of IN cord-329553-93tbgn2b 61 21 DPPIV DPPIV NNP cord-329553-93tbgn2b 61 22 cDNA cdna NN cord-329553-93tbgn2b 61 23 . . . cord-329553-93tbgn2b 62 1 This this DT cord-329553-93tbgn2b 62 2 created create VBD cord-329553-93tbgn2b 62 3 the the DT cord-329553-93tbgn2b 62 4 chimeric chimeric JJ cord-329553-93tbgn2b 62 5 cDNA cdna NN cord-329553-93tbgn2b 62 6 encoding encoding NN cord-329553-93tbgn2b 62 7 for for IN cord-329553-93tbgn2b 62 8 S10- S10- NNP cord-329553-93tbgn2b 63 1 26D. 26d. CD cord-329553-93tbgn2b 63 2 S27 S27 NNP cord-329553-93tbgn2b 63 3 - - HYPH cord-329553-93tbgn2b 63 4 33D. 33d. CD cord-329553-93tbgn2b 63 5 Oligonucleotides Oligonucleotides NNP cord-329553-93tbgn2b 63 6 13 13 CD cord-329553-93tbgn2b 63 7 and and CC cord-329553-93tbgn2b 63 8 14 14 CD cord-329553-93tbgn2b 63 9 were be VBD cord-329553-93tbgn2b 63 10 used use VBN cord-329553-93tbgn2b 63 11 by by IN cord-329553-93tbgn2b 63 12 PCR PCR NNP cord-329553-93tbgn2b 63 13 ( ( -LRB- cord-329553-93tbgn2b 63 14 DPPIV DPPIV NNP cord-329553-93tbgn2b 63 15 cDNA cdna NN cord-329553-93tbgn2b 63 16 as as IN cord-329553-93tbgn2b 63 17 template template NN cord-329553-93tbgn2b 63 18 ) ) -RRB- cord-329553-93tbgn2b 63 19 to to TO cord-329553-93tbgn2b 63 20 create create VB cord-329553-93tbgn2b 63 21 a a DT cord-329553-93tbgn2b 63 22 DNA dna NN cord-329553-93tbgn2b 63 23 fragment fragment NN cord-329553-93tbgn2b 63 24 . . . cord-329553-93tbgn2b 64 1 The the DT cord-329553-93tbgn2b 64 2 PCR PCR NNP cord-329553-93tbgn2b 64 3 product product NN cord-329553-93tbgn2b 64 4 was be VBD cord-329553-93tbgn2b 64 5 digested digest VBN cord-329553-93tbgn2b 64 6 with with IN cord-329553-93tbgn2b 64 7 EcoRI EcoRI NNP cord-329553-93tbgn2b 64 8 and and CC cord-329553-93tbgn2b 64 9 Nod Nod NNP cord-329553-93tbgn2b 64 10 and and CC cord-329553-93tbgn2b 64 11 used use VBD cord-329553-93tbgn2b 64 12 to to TO cord-329553-93tbgn2b 64 13 replace replace VB cord-329553-93tbgn2b 64 14 the the DT cord-329553-93tbgn2b 64 15 EcoRI ecori NN cord-329553-93tbgn2b 64 16 - - HYPH cord-329553-93tbgn2b 64 17 Notl Notl NNP cord-329553-93tbgn2b 64 18 region region NN cord-329553-93tbgn2b 64 19 in in IN cord-329553-93tbgn2b 64 20 pGEM pGEM NNP cord-329553-93tbgn2b 64 21 / / SYM cord-329553-93tbgn2b 64 22 D4S d4s NN cord-329553-93tbgn2b 64 23 . . . cord-329553-93tbgn2b 65 1 The the DT cord-329553-93tbgn2b 65 2 resulting result VBG cord-329553-93tbgn2b 65 3 chimeric chimeric JJ cord-329553-93tbgn2b 65 4 cDNA cdna NN cord-329553-93tbgn2b 65 5 encodes encode VBZ cord-329553-93tbgn2b 65 6 for for IN cord-329553-93tbgn2b 65 7 S27 S27 NNP cord-329553-93tbgn2b 65 8 - - HYPH cord-329553-93tbgn2b 65 9 33D. 33D. NNP cord-329553-93tbgn2b 65 10 Chimeric chimeric JJ cord-329553-93tbgn2b 65 11 cDNAs cDNAs NNPS cord-329553-93tbgn2b 65 12 ( ( -LRB- cord-329553-93tbgn2b 65 13 mainly mainly RB cord-329553-93tbgn2b 65 14 the the DT cord-329553-93tbgn2b 65 15 ST ST NNP cord-329553-93tbgn2b 65 16 and and CC cord-329553-93tbgn2b 65 17 fusion fusion NN cord-329553-93tbgn2b 65 18 regions region NNS cord-329553-93tbgn2b 65 19 ) ) -RRB- cord-329553-93tbgn2b 65 20 were be VBD cord-329553-93tbgn2b 65 21 all all DT cord-329553-93tbgn2b 65 22 confirmed confirm VBN cord-329553-93tbgn2b 65 23 by by IN cord-329553-93tbgn2b 65 24 DNA dna NN cord-329553-93tbgn2b 65 25 sequencing sequencing NN cord-329553-93tbgn2b 65 26 in in IN cord-329553-93tbgn2b 65 27 pGEM pgem JJ cord-329553-93tbgn2b 65 28 vectors vector NNS cord-329553-93tbgn2b 65 29 . . . cord-329553-93tbgn2b 66 1 The the DT cord-329553-93tbgn2b 66 2 resulting result VBG cord-329553-93tbgn2b 66 3 chimeric chimeric JJ cord-329553-93tbgn2b 66 4 cDNAs cdna NNS cord-329553-93tbgn2b 66 5 all all DT cord-329553-93tbgn2b 66 6 have have VBP cord-329553-93tbgn2b 66 7 a a DT cord-329553-93tbgn2b 66 8 unique unique JJ cord-329553-93tbgn2b 66 9 XhoI XhoI NNP cord-329553-93tbgn2b 66 10 site site NN cord-329553-93tbgn2b 66 11 in in IN cord-329553-93tbgn2b 66 12 both both DT cord-329553-93tbgn2b 66 13 5 5 CD cord-329553-93tbgn2b 66 14 ' ' '' cord-329553-93tbgn2b 66 15 and and CC cord-329553-93tbgn2b 66 16 3 3 CD cord-329553-93tbgn2b 66 17 ' ' POS cord-329553-93tbgn2b 66 18 ends end NNS cord-329553-93tbgn2b 66 19 . . . cord-329553-93tbgn2b 67 1 For for IN cord-329553-93tbgn2b 67 2 insertion insertion NN cord-329553-93tbgn2b 67 3 into into IN cord-329553-93tbgn2b 67 4 pRSN prsn NN cord-329553-93tbgn2b 67 5 , , , cord-329553-93tbgn2b 67 6 they -PRON- PRP cord-329553-93tbgn2b 67 7 were be VBD cord-329553-93tbgn2b 67 8 digested digest VBN cord-329553-93tbgn2b 67 9 with with IN cord-329553-93tbgn2b 67 10 Xhol Xhol NNP cord-329553-93tbgn2b 67 11 to to TO cord-329553-93tbgn2b 67 12 retrieve retrieve VB cord-329553-93tbgn2b 67 13 the the DT cord-329553-93tbgn2b 67 14 chimeric chimeric JJ cord-329553-93tbgn2b 67 15 cDNA cdna NN cord-329553-93tbgn2b 67 16 and and CC cord-329553-93tbgn2b 67 17 ligated ligate VBD cord-329553-93tbgn2b 67 18 into into IN cord-329553-93tbgn2b 67 19 the the DT cord-329553-93tbgn2b 67 20 Xhol Xhol NNP cord-329553-93tbgn2b 67 21 site site NN cord-329553-93tbgn2b 67 22 of of IN cord-329553-93tbgn2b 67 23 pRSN prsn NN cord-329553-93tbgn2b 67 24 . . . cord-329553-93tbgn2b 68 1 The the DT cord-329553-93tbgn2b 68 2 right right JJ cord-329553-93tbgn2b 68 3 orientation orientation NN cord-329553-93tbgn2b 68 4 was be VBD cord-329553-93tbgn2b 68 5 verified verify VBN cord-329553-93tbgn2b 68 6 by by IN cord-329553-93tbgn2b 68 7 restriction restriction NN cord-329553-93tbgn2b 68 8 enzyme enzyme NN cord-329553-93tbgn2b 68 9 digestion digestion NN cord-329553-93tbgn2b 68 10 and and CC cord-329553-93tbgn2b 68 11 direct direct JJ cord-329553-93tbgn2b 68 12 DNA dna NN cord-329553-93tbgn2b 68 13 sequencing sequencing NN cord-329553-93tbgn2b 68 14 of of IN cord-329553-93tbgn2b 68 15 the the DT cord-329553-93tbgn2b 68 16 5 5 CD cord-329553-93tbgn2b 68 17 ' ' POS cord-329553-93tbgn2b 68 18 region region NN cord-329553-93tbgn2b 68 19 ( ( -LRB- cord-329553-93tbgn2b 68 20 200 200 CD cord-329553-93tbgn2b 68 21 - - SYM cord-329553-93tbgn2b 68 22 300-bp 300-bp CD cord-329553-93tbgn2b 68 23 sequence sequence NN cord-329553-93tbgn2b 68 24 ) ) -RRB- cord-329553-93tbgn2b 68 25 with with IN cord-329553-93tbgn2b 68 26 oligonucleotide oligonucleotide JJ cord-329553-93tbgn2b 68 27 No no NN cord-329553-93tbgn2b 68 28 . . NN cord-329553-93tbgn2b 68 29 15 15 CD cord-329553-93tbgn2b 68 30 , , , cord-329553-93tbgn2b 68 31 which which WDT cord-329553-93tbgn2b 68 32 hybridizes hybridize VBZ cord-329553-93tbgn2b 68 33 to to IN cord-329553-93tbgn2b 68 34 the the DT cord-329553-93tbgn2b 68 35 sequence sequence NN cord-329553-93tbgn2b 68 36 before before IN cord-329553-93tbgn2b 68 37 the the DT cord-329553-93tbgn2b 68 38 Xhol Xhol NNP cord-329553-93tbgn2b 68 39 site site NN cord-329553-93tbgn2b 68 40 of of IN cord-329553-93tbgn2b 68 41 the the DT cord-329553-93tbgn2b 68 42 pRSN prsn JJ cord-329553-93tbgn2b 68 43 vector vector NN cord-329553-93tbgn2b 68 44 . . . cord-329553-93tbgn2b 69 1 Insertion insertion NN cord-329553-93tbgn2b 69 2 of of IN cord-329553-93tbgn2b 69 3 DPPIV DPPIV NNP cord-329553-93tbgn2b 69 4 cDNA cdna NN cord-329553-93tbgn2b 69 5 into into IN cord-329553-93tbgn2b 69 6 pRSN pRSN NNP cord-329553-93tbgn2b 69 7 has have VBZ cord-329553-93tbgn2b 69 8 been be VBN cord-329553-93tbgn2b 69 9 described describe VBN cord-329553-93tbgn2b 69 10 previously previously RB cord-329553-93tbgn2b 70 1 ( ( -LRB- cord-329553-93tbgn2b 70 2 Low Low NNP cord-329553-93tbgn2b 70 3 et et FW cord-329553-93tbgn2b 70 4 al al NNP cord-329553-93tbgn2b 70 5 . . NNP cord-329553-93tbgn2b 70 6 , , , cord-329553-93tbgn2b 70 7 1991a 1991a CD cord-329553-93tbgn2b 70 8 , , , cord-329553-93tbgn2b 70 9 b b LS cord-329553-93tbgn2b 70 10 ) ) -RRB- cord-329553-93tbgn2b 70 11 . . . cord-329553-93tbgn2b 71 1 Cell cell NN cord-329553-93tbgn2b 71 2 Culture culture NN cord-329553-93tbgn2b 71 3 MDCK MDCK NNP cord-329553-93tbgn2b 72 1 ( ( -LRB- cord-329553-93tbgn2b 72 2 strain strain VB cord-329553-93tbgn2b 72 3 11 11 CD cord-329553-93tbgn2b 72 4 ) ) -RRB- cord-329553-93tbgn2b 72 5 cells cell NNS cord-329553-93tbgn2b 72 6 were be VBD cord-329553-93tbgn2b 72 7 a a DT cord-329553-93tbgn2b 72 8 generous generous JJ cord-329553-93tbgn2b 72 9 gift gift NN cord-329553-93tbgn2b 72 10 from from IN cord-329553-93tbgn2b 72 11 Dr. Dr. NNP cord-329553-93tbgn2b 72 12 K K NNP cord-329553-93tbgn2b 72 13 . . . cord-329553-93tbgn2b 73 1 Simons Simons NNP cord-329553-93tbgn2b 73 2 ( ( -LRB- cord-329553-93tbgn2b 73 3 European European NNP cord-329553-93tbgn2b 73 4 Molecular Molecular NNP cord-329553-93tbgn2b 73 5 Biology Biology NNP cord-329553-93tbgn2b 73 6 Laboratory Laboratory NNP cord-329553-93tbgn2b 73 7 ) ) -RRB- cord-329553-93tbgn2b 73 8 . . . cord-329553-93tbgn2b 74 1 MDCK MDCK NNP cord-329553-93tbgn2b 74 2 and and CC cord-329553-93tbgn2b 74 3 the the DT cord-329553-93tbgn2b 74 4 transfected transfecte VBN cord-329553-93tbgn2b 74 5 cells cell NNS cord-329553-93tbgn2b 74 6 were be VBD cord-329553-93tbgn2b 74 7 cultured culture VBN cord-329553-93tbgn2b 74 8 as as IN cord-329553-93tbgn2b 74 9 before before RB cord-329553-93tbgn2b 75 1 ( ( -LRB- cord-329553-93tbgn2b 75 2 Low Low NNP cord-329553-93tbgn2b 75 3 et et FW cord-329553-93tbgn2b 75 4 al al NNP cord-329553-93tbgn2b 75 5 . . NNP cord-329553-93tbgn2b 75 6 , , , cord-329553-93tbgn2b 75 7 1991b 1991b CD cord-329553-93tbgn2b 75 8 ) ) -RRB- cord-329553-93tbgn2b 75 9 . . . cord-329553-93tbgn2b 76 1 This this DT cord-329553-93tbgn2b 76 2 was be VBD cord-329553-93tbgn2b 76 3 performed perform VBN cord-329553-93tbgn2b 76 4 as as IN cord-329553-93tbgn2b 76 5 described describe VBN cord-329553-93tbgn2b 76 6 previously previously RB cord-329553-93tbgn2b 76 7 ( ( -LRB- cord-329553-93tbgn2b 76 8 Low Low NNP cord-329553-93tbgn2b 76 9 et et FW cord-329553-93tbgn2b 76 10 al al NNP cord-329553-93tbgn2b 76 11 . . NNP cord-329553-93tbgn2b 76 12 , , , cord-329553-93tbgn2b 76 13 1991b 1991b CD cord-329553-93tbgn2b 76 14 ) ) -RRB- cord-329553-93tbgn2b 76 15 . . . cord-329553-93tbgn2b 77 1 Cells cell NNS cord-329553-93tbgn2b 77 2 grown grow VBN cord-329553-93tbgn2b 77 3 on on IN cord-329553-93tbgn2b 77 4 coverslips coverslip NNS cord-329553-93tbgn2b 77 5 were be VBD cord-329553-93tbgn2b 77 6 washed wash VBN cord-329553-93tbgn2b 77 7 twice twice RB cord-329553-93tbgn2b 77 8 with with IN cord-329553-93tbgn2b 77 9 PBS PBS NNP cord-329553-93tbgn2b 77 10 containing contain VBG cord-329553-93tbgn2b 77 11 1 1 CD cord-329553-93tbgn2b 77 12 mM mm CD cord-329553-93tbgn2b 77 13 CaC12 cac12 NN cord-329553-93tbgn2b 77 14 and and CC cord-329553-93tbgn2b 77 15 1 1 CD cord-329553-93tbgn2b 77 16 MM MM NNP cord-329553-93tbgn2b 77 17 M9C12 M9C12 NNP cord-329553-93tbgn2b 77 18 ( ( -LRB- cord-329553-93tbgn2b 77 19 PBSCM PBSCM NNP cord-329553-93tbgn2b 77 20 ) ) -RRB- cord-329553-93tbgn2b 77 21 , , , cord-329553-93tbgn2b 77 22 and and CC cord-329553-93tbgn2b 77 23 then then RB cord-329553-93tbgn2b 77 24 fixed fix VBN cord-329553-93tbgn2b 77 25 with with IN cord-329553-93tbgn2b 77 26 2 2 CD cord-329553-93tbgn2b 77 27 .7 .7 CD cord-329553-93tbgn2b 77 28 % % NN cord-329553-93tbgn2b 77 29 paraformaldehyde paraformaldehyde NN cord-329553-93tbgn2b 77 30 in in IN cord-329553-93tbgn2b 77 31 PBSCM PBSCM NNP cord-329553-93tbgn2b 77 32 at at IN cord-329553-93tbgn2b 77 33 4 4 CD cord-329553-93tbgn2b 77 34 ° ° NN cord-329553-93tbgn2b 77 35 C c NN cord-329553-93tbgn2b 77 36 for for IN cord-329553-93tbgn2b 77 37 60 60 CD cord-329553-93tbgn2b 77 38 min min NN cord-329553-93tbgn2b 77 39 . . . cord-329553-93tbgn2b 78 1 The the DT cord-329553-93tbgn2b 78 2 fixed fix VBN cord-329553-93tbgn2b 78 3 cells cell NNS cord-329553-93tbgn2b 78 4 were be VBD cord-329553-93tbgn2b 78 5 washed wash VBN cord-329553-93tbgn2b 78 6 once once RB cord-329553-93tbgn2b 78 7 with with IN cord-329553-93tbgn2b 78 8 PBSCM PBSCM NNP cord-329553-93tbgn2b 78 9 , , , cord-329553-93tbgn2b 78 10 twice twice RB cord-329553-93tbgn2b 78 11 with with IN cord-329553-93tbgn2b 78 12 PBSCM PBSCM NNP cord-329553-93tbgn2b 78 13 containing contain VBG cord-329553-93tbgn2b 78 14 50 50 CD cord-329553-93tbgn2b 78 15 mM mm CD cord-329553-93tbgn2b 78 16 NH2Cl NH2Cl NNP cord-329553-93tbgn2b 78 17 , , , cord-329553-93tbgn2b 78 18 and and CC cord-329553-93tbgn2b 78 19 then then RB cord-329553-93tbgn2b 78 20 three three CD cord-329553-93tbgn2b 78 21 times time NNS cord-329553-93tbgn2b 78 22 with with IN cord-329553-93tbgn2b 78 23 PBSCM PBSCM NNP cord-329553-93tbgn2b 78 24 ( ( -LRB- cord-329553-93tbgn2b 78 25 5 5 CD cord-329553-93tbgn2b 78 26 - - SYM cord-329553-93tbgn2b 78 27 10 10 CD cord-329553-93tbgn2b 78 28 min min NN cord-329553-93tbgn2b 78 29 each each DT cord-329553-93tbgn2b 78 30 ) ) -RRB- cord-329553-93tbgn2b 78 31 . . . cord-329553-93tbgn2b 79 1 The the DT cord-329553-93tbgn2b 79 2 cells cell NNS cord-329553-93tbgn2b 79 3 were be VBD cord-329553-93tbgn2b 79 4 either either CC cord-329553-93tbgn2b 79 5 not not RB cord-329553-93tbgn2b 79 6 permeabilized permeabilize VBN cord-329553-93tbgn2b 79 7 for for IN cord-329553-93tbgn2b 79 8 surface surface NN cord-329553-93tbgn2b 79 9 staining staining NN cord-329553-93tbgn2b 79 10 or or CC cord-329553-93tbgn2b 79 11 permeabilized permeabilize VBN cord-329553-93tbgn2b 79 12 with with IN cord-329553-93tbgn2b 79 13 0.1 0.1 CD cord-329553-93tbgn2b 79 14 % % NN cord-329553-93tbgn2b 79 15 saponin saponin NN cord-329553-93tbgn2b 79 16 in in IN cord-329553-93tbgn2b 79 17 PBSCM PBSCM NNP cord-329553-93tbgn2b 79 18 for for IN cord-329553-93tbgn2b 79 19 15 15 CD cord-329553-93tbgn2b 79 20 min min NN cord-329553-93tbgn2b 79 21 for for IN cord-329553-93tbgn2b 79 22 total total JJ cord-329553-93tbgn2b 79 23 staining staining NN cord-329553-93tbgn2b 79 24 ( ( -LRB- cord-329553-93tbgn2b 79 25 surface surface NN cord-329553-93tbgn2b 79 26 plus plus CC cord-329553-93tbgn2b 79 27 intracellular intracellular JJ cord-329553-93tbgn2b 79 28 ) ) -RRB- cord-329553-93tbgn2b 79 29 . . . cord-329553-93tbgn2b 80 1 mAbs mAbs NNP cord-329553-93tbgn2b 80 2 ( ( -LRB- cord-329553-93tbgn2b 80 3 ascitic ascitic JJ cord-329553-93tbgn2b 80 4 fluid fluid NNP cord-329553-93tbgn2b 80 5 ) ) -RRB- cord-329553-93tbgn2b 80 6 , , , cord-329553-93tbgn2b 80 7 diluted dilute VBN cord-329553-93tbgn2b 80 8 1 1 CD cord-329553-93tbgn2b 80 9 : : SYM cord-329553-93tbgn2b 80 10 1,000 1,000 CD cord-329553-93tbgn2b 80 11 in in IN cord-329553-93tbgn2b 80 12 fluorescence fluorescence NN cord-329553-93tbgn2b 80 13 dilution dilution NN cord-329553-93tbgn2b 80 14 buffer buffer NN cord-329553-93tbgn2b 80 15 ( ( -LRB- cord-329553-93tbgn2b 80 16 PBSCM PBSCM NNP cord-329553-93tbgn2b 80 17 with with IN cord-329553-93tbgn2b 80 18 5 5 CD cord-329553-93tbgn2b 80 19 % % NN cord-329553-93tbgn2b 80 20 normal normal JJ cord-329553-93tbgn2b 80 21 goat goat NN cord-329553-93tbgn2b 80 22 serum serum NN cord-329553-93tbgn2b 80 23 , , , cord-329553-93tbgn2b 80 24 5 5 CD cord-329553-93tbgn2b 80 25 % % NN cord-329553-93tbgn2b 80 26 FBS FBS NNP cord-329553-93tbgn2b 80 27 , , , cord-329553-93tbgn2b 80 28 and and CC cord-329553-93tbgn2b 80 29 2 2 CD cord-329553-93tbgn2b 80 30 % % NN cord-329553-93tbgn2b 80 31 BSA BSA NNP cord-329553-93tbgn2b 80 32 , , , cord-329553-93tbgn2b 80 33 pH pH NNP cord-329553-93tbgn2b 80 34 7.6 7.6 CD cord-329553-93tbgn2b 80 35 ) ) -RRB- cord-329553-93tbgn2b 80 36 were be VBD cord-329553-93tbgn2b 80 37 added add VBN cord-329553-93tbgn2b 80 38 to to IN cord-329553-93tbgn2b 80 39 the the DT cord-329553-93tbgn2b 80 40 cells cell NNS cord-329553-93tbgn2b 80 41 and and CC cord-329553-93tbgn2b 80 42 incubated incubate VBN cord-329553-93tbgn2b 80 43 at at IN cord-329553-93tbgn2b 80 44 room room NN cord-329553-93tbgn2b 80 45 temperature temperature NN cord-329553-93tbgn2b 80 46 for for IN cord-329553-93tbgn2b 80 47 60 60 CD cord-329553-93tbgn2b 80 48 - - SYM cord-329553-93tbgn2b 80 49 120 120 CD cord-329553-93tbgn2b 80 50 min min NN cord-329553-93tbgn2b 80 51 . . . cord-329553-93tbgn2b 81 1 After after IN cord-329553-93tbgn2b 81 2 washing wash VBG cord-329553-93tbgn2b 81 3 six six CD cord-329553-93tbgn2b 81 4 times time NNS cord-329553-93tbgn2b 81 5 with with IN cord-329553-93tbgn2b 81 6 PBSCM PBSCM NNP cord-329553-93tbgn2b 81 7 ( ( -LRB- cord-329553-93tbgn2b 81 8 5 5 CD cord-329553-93tbgn2b 81 9 - - SYM cord-329553-93tbgn2b 81 10 10 10 CD cord-329553-93tbgn2b 81 11 min min NN cord-329553-93tbgn2b 81 12 each each DT cord-329553-93tbgn2b 81 13 ) ) -RRB- cord-329553-93tbgn2b 81 14 , , , cord-329553-93tbgn2b 81 15 the the DT cord-329553-93tbgn2b 81 16 cells cell NNS cord-329553-93tbgn2b 81 17 were be VBD cord-329553-93tbgn2b 81 18 then then RB cord-329553-93tbgn2b 81 19 incubated incubate VBN cord-329553-93tbgn2b 81 20 with with IN cord-329553-93tbgn2b 81 21 goat goat NN cord-329553-93tbgn2b 81 22 anti anti JJ cord-329553-93tbgn2b 81 23 - - JJ cord-329553-93tbgn2b 81 24 mouse mouse JJ cord-329553-93tbgn2b 81 25 IgG igg NN cord-329553-93tbgn2b 81 26 conjugated conjugate VBN cord-329553-93tbgn2b 81 27 with with IN cord-329553-93tbgn2b 81 28 FIX fix NN cord-329553-93tbgn2b 81 29 ( ( -LRB- cord-329553-93tbgn2b 81 30 10 10 CD cord-329553-93tbgn2b 81 31 pg pg NN cord-329553-93tbgn2b 81 32 / / SYM cord-329553-93tbgn2b 81 33 ml ml NN cord-329553-93tbgn2b 81 34 ) ) -RRB- cord-329553-93tbgn2b 81 35 for for IN cord-329553-93tbgn2b 81 36 60 60 CD cord-329553-93tbgn2b 81 37 - - SYM cord-329553-93tbgn2b 81 38 120 120 CD cord-329553-93tbgn2b 81 39 min min NN cord-329553-93tbgn2b 81 40 . . . cord-329553-93tbgn2b 82 1 After after IN cord-329553-93tbgn2b 82 2 washing washing NN cord-329553-93tbgn2b 82 3 , , , cord-329553-93tbgn2b 82 4 the the DT cord-329553-93tbgn2b 82 5 cells cell NNS cord-329553-93tbgn2b 82 6 were be VBD cord-329553-93tbgn2b 82 7 mounted mount VBN cord-329553-93tbgn2b 82 8 in in IN cord-329553-93tbgn2b 82 9 90 90 CD cord-329553-93tbgn2b 82 10 % % NN cord-329553-93tbgn2b 82 11 glycerol glycerol NN cord-329553-93tbgn2b 82 12 in in IN cord-329553-93tbgn2b 82 13 PBS PBS NNP cord-329553-93tbgn2b 82 14 , , , cord-329553-93tbgn2b 82 15 pH pH NNP cord-329553-93tbgn2b 82 16 8.0 8.0 CD cord-329553-93tbgn2b 82 17 , , , cord-329553-93tbgn2b 82 18 containing contain VBG cord-329553-93tbgn2b 82 19 1 1 CD cord-329553-93tbgn2b 82 20 mg mg NNP cord-329553-93tbgn2b 82 21 / / SYM cord-329553-93tbgn2b 82 22 ml ml NN cord-329553-93tbgn2b 82 23 p p NN cord-329553-93tbgn2b 82 24 - - HYPH cord-329553-93tbgn2b 82 25 phenylenediamine phenylenediamine NN cord-329553-93tbgn2b 82 26 , , , cord-329553-93tbgn2b 82 27 observed observe VBD cord-329553-93tbgn2b 82 28 using use VBG cord-329553-93tbgn2b 82 29 the the DT cord-329553-93tbgn2b 82 30 Axiophot Axiophot NNP cord-329553-93tbgn2b 82 31 microscope microscope NN cord-329553-93tbgn2b 82 32 ( ( -LRB- cord-329553-93tbgn2b 82 33 Carl Carl NNP cord-329553-93tbgn2b 82 34 Zeiss Zeiss NNP cord-329553-93tbgn2b 82 35 , , , cord-329553-93tbgn2b 82 36 Inc Inc NNP cord-329553-93tbgn2b 82 37 . . NNP cord-329553-93tbgn2b 82 38 , , , cord-329553-93tbgn2b 82 39 Thornwood Thornwood NNP cord-329553-93tbgn2b 82 40 , , , cord-329553-93tbgn2b 82 41 NY NY NNP cord-329553-93tbgn2b 82 42 ) ) -RRB- cord-329553-93tbgn2b 82 43 equipped equip VBN cord-329553-93tbgn2b 82 44 with with IN cord-329553-93tbgn2b 82 45 epifluorescence epifluorescence NN cord-329553-93tbgn2b 82 46 optics optic NNS cord-329553-93tbgn2b 82 47 and and CC cord-329553-93tbgn2b 82 48 photographed photograph VBN cord-329553-93tbgn2b 82 49 with with IN cord-329553-93tbgn2b 82 50 Kodak Kodak NNP cord-329553-93tbgn2b 82 51 Tri Tri NNP cord-329553-93tbgn2b 82 52 - - HYPH cord-329553-93tbgn2b 82 53 X X NNP cord-329553-93tbgn2b 82 54 400 400 CD cord-329553-93tbgn2b 82 55 film film NN cord-329553-93tbgn2b 82 56 . . . cord-329553-93tbgn2b 83 1 Diluted Diluted NNP cord-329553-93tbgn2b 83 2 mAbs mAbs NNP cord-329553-93tbgn2b 83 3 and and CC cord-329553-93tbgn2b 83 4 goat goat NNP cord-329553-93tbgn2b 83 5 anti anti JJ cord-329553-93tbgn2b 83 6 - - JJ cord-329553-93tbgn2b 83 7 mouse mouse JJ cord-329553-93tbgn2b 83 8 IgG igg NN cord-329553-93tbgn2b 83 9 conjugated conjugate VBN cord-329553-93tbgn2b 83 10 with with IN cord-329553-93tbgn2b 83 11 FIX fix NN cord-329553-93tbgn2b 83 12 were be VBD cord-329553-93tbgn2b 83 13 spun spin VBN cord-329553-93tbgn2b 83 14 down down RP cord-329553-93tbgn2b 83 15 at at IN cord-329553-93tbgn2b 83 16 4 4 CD cord-329553-93tbgn2b 83 17 ° ° NN cord-329553-93tbgn2b 83 18 C c NN cord-329553-93tbgn2b 83 19 for for IN cord-329553-93tbgn2b 83 20 10 10 CD cord-329553-93tbgn2b 83 21 min min NN cord-329553-93tbgn2b 83 22 ( ( -LRB- cord-329553-93tbgn2b 83 23 14 14 CD cord-329553-93tbgn2b 83 24 krpm krpm NN cord-329553-93tbgn2b 83 25 in in IN cord-329553-93tbgn2b 83 26 a a DT cord-329553-93tbgn2b 83 27 microfuge microfuge NN cord-329553-93tbgn2b 83 28 ) ) -RRB- cord-329553-93tbgn2b 83 29 before before IN cord-329553-93tbgn2b 83 30 use use NN cord-329553-93tbgn2b 83 31 . . . cord-329553-93tbgn2b 84 1 For for IN cord-329553-93tbgn2b 84 2 colocalization colocalization NN cord-329553-93tbgn2b 84 3 with with IN cord-329553-93tbgn2b 84 4 WGA WGA NNP cord-329553-93tbgn2b 84 5 , , , cord-329553-93tbgn2b 84 6 cells cell NNS cord-329553-93tbgn2b 84 7 were be VBD cord-329553-93tbgn2b 84 8 first first RB cord-329553-93tbgn2b 84 9 incubated incubate VBN cord-329553-93tbgn2b 84 10 with with IN cord-329553-93tbgn2b 84 11 0 0 CD cord-329553-93tbgn2b 84 12 .5 .5 . cord-329553-93tbgn2b 85 1 mg mg NNP cord-329553-93tbgn2b 85 2 / / SYM cord-329553-93tbgn2b 85 3 ml ml NNP cord-329553-93tbgn2b 85 4 WGA WGA NNP cord-329553-93tbgn2b 85 5 for for IN cord-329553-93tbgn2b 85 6 60 60 CD cord-329553-93tbgn2b 85 7 min min NN cord-329553-93tbgn2b 85 8 on on IN cord-329553-93tbgn2b 85 9 ice ice NN cord-329553-93tbgn2b 85 10 to to TO cord-329553-93tbgn2b 85 11 block block VB cord-329553-93tbgn2b 85 12 surface surface NN cord-329553-93tbgn2b 85 13 WGA WGA NNP cord-329553-93tbgn2b 85 14 binding bind VBG cord-329553-93tbgn2b 85 15 sites site NNS cord-329553-93tbgn2b 85 16 . . . cord-329553-93tbgn2b 86 1 After after IN cord-329553-93tbgn2b 86 2 a a DT cord-329553-93tbgn2b 86 3 brief brief JJ cord-329553-93tbgn2b 86 4 rinse rinse NN cord-329553-93tbgn2b 86 5 with with IN cord-329553-93tbgn2b 86 6 PBSCM PBSCM NNP cord-329553-93tbgn2b 86 7 , , , cord-329553-93tbgn2b 86 8 the the DT cord-329553-93tbgn2b 86 9 cells cell NNS cord-329553-93tbgn2b 86 10 were be VBD cord-329553-93tbgn2b 86 11 fixed fix VBN cord-329553-93tbgn2b 86 12 and and CC cord-329553-93tbgn2b 86 13 permeabilized permeabilize VBN cord-329553-93tbgn2b 86 14 as as IN cord-329553-93tbgn2b 86 15 above above RB cord-329553-93tbgn2b 86 16 . . . cord-329553-93tbgn2b 87 1 Cells cell NNS cord-329553-93tbgn2b 87 2 were be VBD cord-329553-93tbgn2b 87 3 then then RB cord-329553-93tbgn2b 87 4 incubated incubate VBN cord-329553-93tbgn2b 87 5 with with IN cord-329553-93tbgn2b 87 6 rhodamine rhodamine NN cord-329553-93tbgn2b 87 7 - - HYPH cord-329553-93tbgn2b 87 8 WGA WGA NNP cord-329553-93tbgn2b 87 9 ( ( -LRB- cord-329553-93tbgn2b 87 10 2 2 CD cord-329553-93tbgn2b 87 11 .5 .5 CD cord-329553-93tbgn2b 87 12 mg mg NNP cord-329553-93tbgn2b 87 13 / / SYM cord-329553-93tbgn2b 87 14 ml ml NNP cord-329553-93tbgn2b 87 15 ) ) -RRB- cord-329553-93tbgn2b 87 16 in in IN cord-329553-93tbgn2b 87 17 PBSCM PBSCM NNP cord-329553-93tbgn2b 87 18 for for IN cord-329553-93tbgn2b 87 19 60 60 CD cord-329553-93tbgn2b 87 20 min min NN cord-329553-93tbgn2b 87 21 . . . cord-329553-93tbgn2b 88 1 After after IN cord-329553-93tbgn2b 88 2 washing washing NN cord-329553-93tbgn2b 88 3 , , , cord-329553-93tbgn2b 88 4 the the DT cord-329553-93tbgn2b 88 5 cells cell NNS cord-329553-93tbgn2b 88 6 were be VBD cord-329553-93tbgn2b 88 7 fixed fix VBN cord-329553-93tbgn2b 88 8 again again RB cord-329553-93tbgn2b 88 9 to to TO cord-329553-93tbgn2b 88 10 immobilize immobilize VB cord-329553-93tbgn2b 88 11 the the DT cord-329553-93tbgn2b 88 12 bound bind VBN cord-329553-93tbgn2b 88 13 rhodamine rhodamine NN cord-329553-93tbgn2b 88 14 -- -- : cord-329553-93tbgn2b 88 15 WGA WGA NNP cord-329553-93tbgn2b 88 16 . . . cord-329553-93tbgn2b 89 1 After after IN cord-329553-93tbgn2b 89 2 WGA WGA NNP cord-329553-93tbgn2b 89 3 staining staining NN cord-329553-93tbgn2b 89 4 , , , cord-329553-93tbgn2b 89 5 the the DT cord-329553-93tbgn2b 89 6 cells cell NNS cord-329553-93tbgn2b 89 7 were be VBD cord-329553-93tbgn2b 89 8 sequentially sequentially RB cord-329553-93tbgn2b 89 9 incubated incubate VBN cord-329553-93tbgn2b 89 10 with with IN cord-329553-93tbgn2b 89 11 monoclonal monoclonal JJ cord-329553-93tbgn2b 89 12 antibodies antibody NNS cord-329553-93tbgn2b 89 13 against against IN cord-329553-93tbgn2b 89 14 DPPIV DPPIV NNP cord-329553-93tbgn2b 89 15 and and CC cord-329553-93tbgn2b 89 16 goat goat NN cord-329553-93tbgn2b 89 17 anti anti JJ cord-329553-93tbgn2b 89 18 - - JJ cord-329553-93tbgn2b 89 19 mouse mouse JJ cord-329553-93tbgn2b 89 20 IgG igg NN cord-329553-93tbgn2b 89 21 - - HYPH cord-329553-93tbgn2b 89 22 FITC FITC NNP cord-329553-93tbgn2b 89 23 . . . cord-329553-93tbgn2b 90 1 The the DT cord-329553-93tbgn2b 90 2 coverslips coverslip NNS cord-329553-93tbgn2b 90 3 were be VBD cord-329553-93tbgn2b 90 4 then then RB cord-329553-93tbgn2b 90 5 processed process VBN cord-329553-93tbgn2b 90 6 and and CC cord-329553-93tbgn2b 90 7 observed observe VBD cord-329553-93tbgn2b 90 8 as as IN cord-329553-93tbgn2b 90 9 above above RB cord-329553-93tbgn2b 90 10 . . . cord-329553-93tbgn2b 91 1 Cells cell NNS cord-329553-93tbgn2b 91 2 were be VBD cord-329553-93tbgn2b 91 3 washed wash VBN cord-329553-93tbgn2b 91 4 twice twice RB cord-329553-93tbgn2b 91 5 with with IN cord-329553-93tbgn2b 91 6 PBSCM PBSCM NNP cord-329553-93tbgn2b 91 7 and and CC cord-329553-93tbgn2b 91 8 then then RB cord-329553-93tbgn2b 91 9 incubated incubate VBD cord-329553-93tbgn2b 91 10 for for IN cord-329553-93tbgn2b 91 11 45 45 CD cord-329553-93tbgn2b 91 12 nun nun NN cord-329553-93tbgn2b 91 13 at at IN cord-329553-93tbgn2b 91 14 37 37 CD cord-329553-93tbgn2b 91 15 ° ° NN cord-329553-93tbgn2b 91 16 C C NNP cord-329553-93tbgn2b 91 17 inmethionine inmethionine NN cord-329553-93tbgn2b 91 18 - - HYPH cord-329553-93tbgn2b 91 19 freemedium freemedium NN cord-329553-93tbgn2b 91 20 supplemented supplement VBN cord-329553-93tbgn2b 91 21 with with IN cord-329553-93tbgn2b 91 22 10 10 CD cord-329553-93tbgn2b 91 23 % % NN cord-329553-93tbgn2b 91 24 dialyzed dialyze VBN cord-329553-93tbgn2b 91 25 FBS FBS NNP cord-329553-93tbgn2b 91 26 ( ( -LRB- cord-329553-93tbgn2b 91 27 labeling labeling NN cord-329553-93tbgn2b 91 28 buffer buffer NN cord-329553-93tbgn2b 91 29 ) ) -RRB- cord-329553-93tbgn2b 91 30 . . . cord-329553-93tbgn2b 92 1 The the DT cord-329553-93tbgn2b 92 2 cells cell NNS cord-329553-93tbgn2b 92 3 were be VBD cord-329553-93tbgn2b 92 4 then then RB cord-329553-93tbgn2b 92 5 pulse pulse NN cord-329553-93tbgn2b 92 6 - - HYPH cord-329553-93tbgn2b 92 7 labeled label VBN cord-329553-93tbgn2b 92 8 with with IN cord-329553-93tbgn2b 92 9 [ [ -LRB- cord-329553-93tbgn2b 92 10 35 35 CD cord-329553-93tbgn2b 92 11 S]methionine S]methionine NNP cord-329553-93tbgn2b 92 12 ( ( -LRB- cord-329553-93tbgn2b 92 13 1 1 CD cord-329553-93tbgn2b 92 14 mCi mCi NNS cord-329553-93tbgn2b 92 15 / / SYM cord-329553-93tbgn2b 92 16 ml ml NNS cord-329553-93tbgn2b 92 17 in in IN cord-329553-93tbgn2b 92 18 labeling labeling NN cord-329553-93tbgn2b 92 19 buffer buffer NN cord-329553-93tbgn2b 92 20 ) ) -RRB- cord-329553-93tbgn2b 92 21 , , , cord-329553-93tbgn2b 92 22 washed wash VBN cord-329553-93tbgn2b 92 23 , , , cord-329553-93tbgn2b 92 24 and and CC cord-329553-93tbgn2b 92 25 chased chase VBN cord-329553-93tbgn2b 92 26 in in IN cord-329553-93tbgn2b 92 27 medium medium JJ cord-329553-93tbgn2b 92 28 containing contain VBG cord-329553-93tbgn2b 92 29 excess excess JJ cord-329553-93tbgn2b 92 30 cold cold JJ cord-329553-93tbgn2b 92 31 methionine methionine NN cord-329553-93tbgn2b 92 32 ( ( -LRB- cord-329553-93tbgn2b 92 33 100 100 CD cord-329553-93tbgn2b 92 34 mg mg NN cord-329553-93tbgn2b 92 35 / / SYM cord-329553-93tbgn2b 92 36 liter liter NN cord-329553-93tbgn2b 92 37 ) ) -RRB- cord-329553-93tbgn2b 92 38 for for IN cord-329553-93tbgn2b 92 39 various various JJ cord-329553-93tbgn2b 92 40 times time NNS cord-329553-93tbgn2b 92 41 as as IN cord-329553-93tbgn2b 92 42 detailed detail VBN cord-329553-93tbgn2b 92 43 in in IN cord-329553-93tbgn2b 92 44 each each DT cord-329553-93tbgn2b 92 45 figure figure NN cord-329553-93tbgn2b 92 46 . . . cord-329553-93tbgn2b 93 1 For for IN cord-329553-93tbgn2b 93 2 assessing assess VBG cord-329553-93tbgn2b 93 3 the the DT cord-329553-93tbgn2b 93 4 surface surface NN cord-329553-93tbgn2b 93 5 expression expression NN cord-329553-93tbgn2b 93 6 , , , cord-329553-93tbgn2b 93 7 cells cell NNS cord-329553-93tbgn2b 93 8 were be VBD cord-329553-93tbgn2b 93 9 grown grow VBN cord-329553-93tbgn2b 93 10 on on IN cord-329553-93tbgn2b 93 11 Transwell Transwell NNP cord-329553-93tbgn2b 93 12 filters filter NNS cord-329553-93tbgn2b 93 13 so so IN cord-329553-93tbgn2b 93 14 that that IN cord-329553-93tbgn2b 93 15 the the DT cord-329553-93tbgn2b 93 16 entire entire JJ cord-329553-93tbgn2b 93 17 surface surface NN cord-329553-93tbgn2b 93 18 was be VBD cord-329553-93tbgn2b 93 19 maximally maximally RB cord-329553-93tbgn2b 93 20 exposed expose VBN cord-329553-93tbgn2b 93 21 for for IN cord-329553-93tbgn2b 93 22 biotinylation biotinylation NN cord-329553-93tbgn2b 93 23 . . . cord-329553-93tbgn2b 94 1 Cells cell NNS cord-329553-93tbgn2b 94 2 grown grow VBN cord-329553-93tbgn2b 94 3 on on IN cord-329553-93tbgn2b 94 4 Transwell Transwell NNP cord-329553-93tbgn2b 94 5 filters filter NNS cord-329553-93tbgn2b 94 6 were be VBD cord-329553-93tbgn2b 94 7 similarly similarly RB cord-329553-93tbgn2b 94 8 labeled label VBN cord-329553-93tbgn2b 94 9 with with IN cord-329553-93tbgn2b 94 10 ( ( -LRB- cord-329553-93tbgn2b 94 11 35 35 CD cord-329553-93tbgn2b 94 12 S]methionine S]methionine NNP cord-329553-93tbgn2b 94 13 . . . cord-329553-93tbgn2b 95 1 This this DT cord-329553-93tbgn2b 95 2 was be VBD cord-329553-93tbgn2b 95 3 performed perform VBN cord-329553-93tbgn2b 95 4 as as IN cord-329553-93tbgn2b 95 5 described describe VBN cord-329553-93tbgn2b 95 6 ( ( -LRB- cord-329553-93tbgn2b 95 7 Le Le NNP cord-329553-93tbgn2b 95 8 Bivic Bivic NNP cord-329553-93tbgn2b 95 9 et et FW cord-329553-93tbgn2b 95 10 al al NNP cord-329553-93tbgn2b 95 11 . . . cord-329553-93tbgn2b 96 1 , , , cord-329553-93tbgn2b 96 2 1990a 1990a CD cord-329553-93tbgn2b 96 3 , , , cord-329553-93tbgn2b 96 4 b b LS cord-329553-93tbgn2b 96 5 ; ; : cord-329553-93tbgn2b 96 6 Low Low NNP cord-329553-93tbgn2b 96 7 et et FW cord-329553-93tbgn2b 96 8 al al NNP cord-329553-93tbgn2b 96 9 . . NNP cord-329553-93tbgn2b 96 10 , , , cord-329553-93tbgn2b 96 11 1991a 1991a CD cord-329553-93tbgn2b 96 12 , , , cord-329553-93tbgn2b 96 13 b b LS cord-329553-93tbgn2b 96 14 ) ) -RRB- cord-329553-93tbgn2b 96 15 . . . cord-329553-93tbgn2b 97 1 Briefly briefly RB cord-329553-93tbgn2b 97 2 , , , cord-329553-93tbgn2b 97 3 cells cell NNS cord-329553-93tbgn2b 97 4 grown grow VBN cord-329553-93tbgn2b 97 5 on on IN cord-329553-93tbgn2b 97 6 Transwell Transwell NNP cord-329553-93tbgn2b 97 7 filters filter NNS cord-329553-93tbgn2b 97 8 were be VBD cord-329553-93tbgn2b 97 9 washed wash VBN cord-329553-93tbgn2b 97 10 four four CD cord-329553-93tbgn2b 97 11 times time NNS cord-329553-93tbgn2b 97 12 with with IN cord-329553-93tbgn2b 97 13 PBSCM PBSCM NNP cord-329553-93tbgn2b 97 14 ( ( -LRB- cord-329553-93tbgn2b 97 15 5 5 CD cord-329553-93tbgn2b 97 16 - - SYM cord-329553-93tbgn2b 97 17 10 10 CD cord-329553-93tbgn2b 97 18 min min NN cord-329553-93tbgn2b 97 19 each each DT cord-329553-93tbgn2b 97 20 ) ) -RRB- cord-329553-93tbgn2b 97 21 on on IN cord-329553-93tbgn2b 97 22 ice ice NN cord-329553-93tbgn2b 97 23 . . . cord-329553-93tbgn2b 98 1 The the DT cord-329553-93tbgn2b 98 2 entire entire JJ cord-329553-93tbgn2b 98 3 surface surface NN cord-329553-93tbgn2b 98 4 was be VBD cord-329553-93tbgn2b 98 5 biotinylated biotinylate VBN cord-329553-93tbgn2b 98 6 by by IN cord-329553-93tbgn2b 98 7 adding add VBG cord-329553-93tbgn2b 98 8 to to IN cord-329553-93tbgn2b 98 9 both both CC cord-329553-93tbgn2b 98 10 the the DT cord-329553-93tbgn2b 98 11 upperand upperand NN cord-329553-93tbgn2b 98 12 lower low JJR cord-329553-93tbgn2b 98 13 chambers chamber NNS cord-329553-93tbgn2b 98 14 1 1 CD cord-329553-93tbgn2b 98 15 ail ail NN cord-329553-93tbgn2b 98 16 of of IN cord-329553-93tbgn2b 98 17 PBSCM PBSCM NNP cord-329553-93tbgn2b 98 18 containing contain VBG cord-329553-93tbgn2b 98 19 s s NNP cord-329553-93tbgn2b 98 20 - - HYPH cord-329553-93tbgn2b 98 21 NHS NHS NNP cord-329553-93tbgn2b 98 22 - - HYPH cord-329553-93tbgn2b 98 23 biotin biotin NNP cord-329553-93tbgn2b 98 24 ( ( -LRB- cord-329553-93tbgn2b 98 25 0.5 0.5 CD cord-329553-93tbgn2b 98 26 mg mg NNP cord-329553-93tbgn2b 98 27 / / SYM cord-329553-93tbgn2b 98 28 ml ml NN cord-329553-93tbgn2b 98 29 diluted dilute VBD cord-329553-93tbgn2b 98 30 from from IN cord-329553-93tbgn2b 98 31 200 200 CD cord-329553-93tbgn2b 98 32 mg mg NNP cord-329553-93tbgn2b 98 33 / / SYM cord-329553-93tbgn2b 98 34 ml ml NN cord-329553-93tbgn2b 98 35 stock stock NN cord-329553-93tbgn2b 98 36 in in IN cord-329553-93tbgn2b 98 37 DMSO DMSO NNP cord-329553-93tbgn2b 98 38 ) ) -RRB- cord-329553-93tbgn2b 98 39 . . . cord-329553-93tbgn2b 99 1 The the DT cord-329553-93tbgn2b 99 2 biotinylation biotinylation NN cord-329553-93tbgn2b 99 3 was be VBD cord-329553-93tbgn2b 99 4 performed perform VBN cord-329553-93tbgn2b 99 5 twice twice RB cord-329553-93tbgn2b 99 6 on on IN cord-329553-93tbgn2b 99 7 ice ice NN cord-329553-93tbgn2b 99 8 ( ( -LRB- cord-329553-93tbgn2b 99 9 15 15 CD cord-329553-93tbgn2b 99 10 - - SYM cord-329553-93tbgn2b 99 11 20 20 CD cord-329553-93tbgn2b 99 12 min min NN cord-329553-93tbgn2b 99 13 each each DT cord-329553-93tbgn2b 99 14 ) ) -RRB- cord-329553-93tbgn2b 99 15 and and CC cord-329553-93tbgn2b 99 16 stopped stop VBD cord-329553-93tbgn2b 99 17 by by IN cord-329553-93tbgn2b 99 18 repeated repeat VBN cord-329553-93tbgn2b 99 19 washing washing NN cord-329553-93tbgn2b 99 20 with with IN cord-329553-93tbgn2b 99 21 PBSCM PBSCM NNP cord-329553-93tbgn2b 99 22 containing contain VBG cord-329553-93tbgn2b 99 23 50 50 CD cord-329553-93tbgn2b 99 24 mM mm CD cord-329553-93tbgn2b 99 25 NH4Cl nh4cl CD cord-329553-93tbgn2b 99 26 and and CC cord-329553-93tbgn2b 99 27 medium medium NN cord-329553-93tbgn2b 99 28 with with IN cord-329553-93tbgn2b 99 29 10 10 CD cord-329553-93tbgn2b 99 30 % % NN cord-329553-93tbgn2b 99 31 FBS FBS NNP cord-329553-93tbgn2b 99 32 . . . cord-329553-93tbgn2b 100 1 These these DT cord-329553-93tbgn2b 100 2 were be VBD cord-329553-93tbgn2b 100 3 performed perform VBN cord-329553-93tbgn2b 100 4 exactly exactly RB cord-329553-93tbgn2b 100 5 as as IN cord-329553-93tbgn2b 100 6 described describe VBN cord-329553-93tbgn2b 100 7 previously previously RB cord-329553-93tbgn2b 100 8 ( ( -LRB- cord-329553-93tbgn2b 100 9 Low Low NNP cord-329553-93tbgn2b 100 10 et et FW cord-329553-93tbgn2b 100 11 al al NNP cord-329553-93tbgn2b 100 12 . . NNP cord-329553-93tbgn2b 100 13 , , , cord-329553-93tbgn2b 100 14 1991a 1991a CD cord-329553-93tbgn2b 100 15 , , , cord-329553-93tbgn2b 100 16 b b LS cord-329553-93tbgn2b 100 17 ) ) -RRB- cord-329553-93tbgn2b 100 18 . . . cord-329553-93tbgn2b 101 1 The the DT cord-329553-93tbgn2b 101 2 immunocomplex immunocomplex NN cord-329553-93tbgn2b 101 3 on on IN cord-329553-93tbgn2b 101 4 Sepharose Sepharose NNP cord-329553-93tbgn2b 101 5 beads bead NNS cord-329553-93tbgn2b 101 6 was be VBD cord-329553-93tbgn2b 101 7 resuspended resuspend VBN cord-329553-93tbgn2b 101 8 in in IN cord-329553-93tbgn2b 101 9 300 300 CD cord-329553-93tbgn2b 101 10 Al Al NNP cord-329553-93tbgn2b 101 11 of of IN cord-329553-93tbgn2b 101 12 0.1 0.1 CD cord-329553-93tbgn2b 101 13 M M NNP cord-329553-93tbgn2b 101 14 sodium sodium NN cord-329553-93tbgn2b 101 15 citrate citrate NN cord-329553-93tbgn2b 101 16 , , , cord-329553-93tbgn2b 101 17 pH pH NNP cord-329553-93tbgn2b 101 18 5.5 5.5 CD cord-329553-93tbgn2b 101 19 , , , cord-329553-93tbgn2b 101 20 0.1 0.1 CD cord-329553-93tbgn2b 101 21 % % NN cord-329553-93tbgn2b 101 22 Triton Triton NNP cord-329553-93tbgn2b 101 23 X-100 x-100 NN cord-329553-93tbgn2b 101 24 , , , cord-329553-93tbgn2b 101 25 0.1 0.1 CD cord-329553-93tbgn2b 101 26 % % NN cord-329553-93tbgn2b 101 27 0-mercaptoethanol 0-mercaptoethanol CD cord-329553-93tbgn2b 101 28 , , , cord-329553-93tbgn2b 101 29 100 100 CD cord-329553-93tbgn2b 101 30 mg mg NNP cord-329553-93tbgn2b 101 31 / / SYM cord-329553-93tbgn2b 101 32 ml ml NNP cord-329553-93tbgn2b 101 33 aprotinin aprotinin NNP cord-329553-93tbgn2b 101 34 , , , cord-329553-93tbgn2b 101 35 and and CC cord-329553-93tbgn2b 101 36 1 1 CD cord-329553-93tbgn2b 101 37 mM mm CD cord-329553-93tbgn2b 101 38 CaC12 cac12 NN cord-329553-93tbgn2b 101 39 . . . cord-329553-93tbgn2b 102 1 10 10 CD cord-329553-93tbgn2b 102 2 mU mU NNS cord-329553-93tbgn2b 102 3 of of IN cord-329553-93tbgn2b 102 4 endo endo NN cord-329553-93tbgn2b 102 5 H H NNP cord-329553-93tbgn2b 102 6 was be VBD cord-329553-93tbgn2b 102 7 then then RB cord-329553-93tbgn2b 102 8 added add VBN cord-329553-93tbgn2b 102 9 . . . cord-329553-93tbgn2b 103 1 After after IN cord-329553-93tbgn2b 103 2 overnight overnight JJ cord-329553-93tbgn2b 103 3 incubation incubation NN cord-329553-93tbgn2b 103 4 at at IN cord-329553-93tbgn2b 103 5 37 37 CD cord-329553-93tbgn2b 103 6 ° ° NN cord-329553-93tbgn2b 103 7 C c NN cord-329553-93tbgn2b 103 8 , , , cord-329553-93tbgn2b 103 9 the the DT cord-329553-93tbgn2b 103 10 beads bead NNS cord-329553-93tbgn2b 103 11 were be VBD cord-329553-93tbgn2b 103 12 recovered recover VBN cord-329553-93tbgn2b 103 13 and and CC cord-329553-93tbgn2b 103 14 lysed lyse VBN cord-329553-93tbgn2b 103 15 in in IN cord-329553-93tbgn2b 103 16 80 80 CD cord-329553-93tbgn2b 103 17 Al Al NNP cord-329553-93tbgn2b 103 18 SDS SDS NNP cord-329553-93tbgn2b 103 19 sample sample NN cord-329553-93tbgn2b 103 20 buffer buffer NN cord-329553-93tbgn2b 103 21 followed follow VBN cord-329553-93tbgn2b 103 22 by by IN cord-329553-93tbgn2b 103 23 SDS SDS NNP cord-329553-93tbgn2b 103 24 - - HYPH cord-329553-93tbgn2b 103 25 PAGE PAGE NNP cord-329553-93tbgn2b 103 26 . . . cord-329553-93tbgn2b 104 1 Immunoprecipitated immunoprecipitated JJ cord-329553-93tbgn2b 104 2 proteins protein NNS cord-329553-93tbgn2b 104 3 were be VBD cord-329553-93tbgn2b 104 4 eluted elute VBN cord-329553-93tbgn2b 104 5 from from IN cord-329553-93tbgn2b 104 6 beads bead NNS cord-329553-93tbgn2b 104 7 by by IN cord-329553-93tbgn2b 104 8 boiling boil VBG cord-329553-93tbgn2b 104 9 in in RP cord-329553-93tbgn2b 104 10 80 80 CD cord-329553-93tbgn2b 104 11 Al Al NNP cord-329553-93tbgn2b 104 12 SDS SDS NNP cord-329553-93tbgn2b 104 13 sample sample NN cord-329553-93tbgn2b 104 14 buffer buffer NN cord-329553-93tbgn2b 104 15 ( ( -LRB- cord-329553-93tbgn2b 104 16 without without IN cord-329553-93tbgn2b 104 17 ß ß NN cord-329553-93tbgn2b 104 18 - - HYPH cord-329553-93tbgn2b 104 19 mercaptoethanol mercaptoethanol NN cord-329553-93tbgn2b 104 20 ) ) -RRB- cord-329553-93tbgn2b 104 21 followed follow VBN cord-329553-93tbgn2b 104 22 by by IN cord-329553-93tbgn2b 104 23 dilution dilution NN cord-329553-93tbgn2b 104 24 in in IN cord-329553-93tbgn2b 104 25 several several JJ cord-329553-93tbgn2b 104 26 milliliters milliliter NNS cord-329553-93tbgn2b 104 27 of of IN cord-329553-93tbgn2b 104 28 lectin lectin NN cord-329553-93tbgn2b 104 29 buffer buffer NN cord-329553-93tbgn2b 104 30 ( ( -LRB- cord-329553-93tbgn2b 104 31 40 40 CD cord-329553-93tbgn2b 104 32 mM mM NNP cord-329553-93tbgn2b 104 33 Tris Tris NNP cord-329553-93tbgn2b 104 34 - - HYPH cord-329553-93tbgn2b 104 35 HCI HCI NNP cord-329553-93tbgn2b 104 36 , , , cord-329553-93tbgn2b 104 37 pH pH NNP cord-329553-93tbgn2b 104 38 7.5 7.5 CD cord-329553-93tbgn2b 104 39 , , , cord-329553-93tbgn2b 104 40 150 150 CD cord-329553-93tbgn2b 104 41 mM mm CD cord-329553-93tbgn2b 104 42 NaCl nacl NN cord-329553-93tbgn2b 104 43 , , , cord-329553-93tbgn2b 104 44 1 1 CD cord-329553-93tbgn2b 104 45 mM mm CD cord-329553-93tbgn2b 104 46 CaC12 cac12 NN cord-329553-93tbgn2b 104 47 , , , cord-329553-93tbgn2b 104 48 1 1 CD cord-329553-93tbgn2b 104 49 mM mm CD cord-329553-93tbgn2b 104 50 MgC12 MgC12 NNP cord-329553-93tbgn2b 104 51 , , , cord-329553-93tbgn2b 104 52 and and CC cord-329553-93tbgn2b 104 53 1 1 CD cord-329553-93tbgn2b 104 54 MM MM NNP cord-329553-93tbgn2b 104 55 MnC12 MnC12 NNP cord-329553-93tbgn2b 104 56 ) ) -RRB- cord-329553-93tbgn2b 104 57 . . . cord-329553-93tbgn2b 105 1 An an DT cord-329553-93tbgn2b 105 2 aliquot aliquot NN cord-329553-93tbgn2b 105 3 of of IN cord-329553-93tbgn2b 105 4 this this DT cord-329553-93tbgn2b 105 5 was be VBD cord-329553-93tbgn2b 105 6 incubated incubate VBN cord-329553-93tbgn2b 105 7 withthe withthe DT cord-329553-93tbgn2b 105 8 various various JJ cord-329553-93tbgn2b 105 9 lectin lectin NN cord-329553-93tbgn2b 105 10 - - HYPH cord-329553-93tbgn2b 105 11 agaroses agarose NNS cord-329553-93tbgn2b 105 12 ( ( -LRB- cord-329553-93tbgn2b 105 13 120 120 CD cord-329553-93tbgn2b 105 14 Al Al NNP cord-329553-93tbgn2b 105 15 slurry slurry NN cord-329553-93tbgn2b 105 16 ) ) -RRB- cord-329553-93tbgn2b 105 17 for for IN cord-329553-93tbgn2b 105 18 60 60 CD cord-329553-93tbgn2b 105 19 - - SYM cord-329553-93tbgn2b 105 20 120 120 CD cord-329553-93tbgn2b 105 21 min min NN cord-329553-93tbgn2b 105 22 . . . cord-329553-93tbgn2b 106 1 After after IN cord-329553-93tbgn2b 106 2 extensive extensive JJ cord-329553-93tbgn2b 106 3 washing washing NN cord-329553-93tbgn2b 106 4 , , , cord-329553-93tbgn2b 106 5 the the DT cord-329553-93tbgn2b 106 6 bound bind VBN cord-329553-93tbgn2b 106 7 materials material NNS cord-329553-93tbgn2b 106 8 were be VBD cord-329553-93tbgn2b 106 9 eluted elute VBN cord-329553-93tbgn2b 106 10 by by IN cord-329553-93tbgn2b 106 11 boiling boil VBG cord-329553-93tbgn2b 106 12 in in IN cord-329553-93tbgn2b 106 13 SDS SDS NNP cord-329553-93tbgn2b 106 14 sample sample NN cord-329553-93tbgn2b 106 15 buffer buffer NN cord-329553-93tbgn2b 106 16 and and CC cord-329553-93tbgn2b 106 17 analyzed analyze VBN cord-329553-93tbgn2b 106 18 by by IN cord-329553-93tbgn2b 106 19 SDS SDS NNP cord-329553-93tbgn2b 106 20 - - HYPH cord-329553-93tbgn2b 106 21 PAGE PAGE NNP cord-329553-93tbgn2b 106 22 . . . cord-329553-93tbgn2b 107 1 In in IN cord-329553-93tbgn2b 107 2 Vitro Vitro NNP cord-329553-93tbgn2b 107 3 Translation Translation NNP cord-329553-93tbgn2b 107 4 and and CC cord-329553-93tbgn2b 107 5 Sodium Sodium NNP cord-329553-93tbgn2b 107 6 Bicarbonate Bicarbonate NNP cord-329553-93tbgn2b 107 7 Treatment Treatment NNP cord-329553-93tbgn2b 108 1 These these DT cord-329553-93tbgn2b 108 2 were be VBD cord-329553-93tbgn2b 108 3 performed perform VBN cord-329553-93tbgn2b 108 4 as as IN cord-329553-93tbgn2b 108 5 described describe VBN cord-329553-93tbgn2b 108 6 previously previously RB cord-329553-93tbgn2b 108 7 ( ( -LRB- cord-329553-93tbgn2b 108 8 Hong Hong NNP cord-329553-93tbgn2b 108 9 and and CC cord-329553-93tbgn2b 108 10 Doyle Doyle NNP cord-329553-93tbgn2b 108 11 , , , cord-329553-93tbgn2b 108 12 1990 1990 CD cord-329553-93tbgn2b 108 13 ) ) -RRB- cord-329553-93tbgn2b 108 14 . . . cord-329553-93tbgn2b 109 1 These these DT cord-329553-93tbgn2b 109 2 were be VBD cord-329553-93tbgn2b 109 3 carried carry VBN cord-329553-93tbgn2b 109 4 out out RP cord-329553-93tbgn2b 109 5 exactly exactly RB cord-329553-93tbgn2b 109 6 as as IN cord-329553-93tbgn2b 109 7 described describe VBN cord-329553-93tbgn2b 109 8 before before IN cord-329553-93tbgn2b 109 9 ( ( -LRB- cord-329553-93tbgn2b 109 10 Low Low NNP cord-329553-93tbgn2b 109 11 et et FW cord-329553-93tbgn2b 109 12 al al NNP cord-329553-93tbgn2b 109 13 . . NNP cord-329553-93tbgn2b 109 14 , , , cord-329553-93tbgn2b 109 15 1991a 1991a CD cord-329553-93tbgn2b 109 16 ) ) -RRB- cord-329553-93tbgn2b 109 17 . . . cord-329553-93tbgn2b 110 1 Localization localization NN cord-329553-93tbgn2b 110 2 ofS33D ofS33D NNP cord-329553-93tbgn2b 110 3 and and CC cord-329553-93tbgn2b 110 4 S64D s64d NN cord-329553-93tbgn2b 110 5 to to IN cord-329553-93tbgn2b 110 6 the the DT cord-329553-93tbgn2b 110 7 GolgiApparatus GolgiApparatus NNP cord-329553-93tbgn2b 110 8 ST ST NNP cord-329553-93tbgn2b 110 9 is be VBZ cord-329553-93tbgn2b 110 10 a a DT cord-329553-93tbgn2b 110 11 47 47 CD cord-329553-93tbgn2b 110 12 - - SYM cord-329553-93tbgn2b 110 13 48-kD 48-kd CD cord-329553-93tbgn2b 110 14 integral integral JJ cord-329553-93tbgn2b 110 15 membrane membrane NN cord-329553-93tbgn2b 110 16 protein protein NN cord-329553-93tbgn2b 110 17 oftype oftype NNP cord-329553-93tbgn2b 110 18 II II NNP cord-329553-93tbgn2b 110 19 orientation orientation NN cord-329553-93tbgn2b 110 20 ( ( -LRB- cord-329553-93tbgn2b 110 21 single single JJ cord-329553-93tbgn2b 110 22 transmembrane transmembrane NN cord-329553-93tbgn2b 110 23 domain domain NN cord-329553-93tbgn2b 110 24 with with IN cord-329553-93tbgn2b 110 25 its -PRON- PRP$ cord-329553-93tbgn2b 110 26 NH2 NH2 NNP cord-329553-93tbgn2b 110 27 terminus terminus NN cord-329553-93tbgn2b 110 28 in in IN cord-329553-93tbgn2b 110 29 the the DT cord-329553-93tbgn2b 110 30 cytosol cytosol NN cord-329553-93tbgn2b 110 31 and and CC cord-329553-93tbgn2b 110 32 the the DT cord-329553-93tbgn2b 110 33 COOH COOH NNP cord-329553-93tbgn2b 110 34 terminus terminus NN cord-329553-93tbgn2b 110 35 in in IN cord-329553-93tbgn2b 110 36 the the DT cord-329553-93tbgn2b 110 37 lumen lumen NN cord-329553-93tbgn2b 110 38 of of IN cord-329553-93tbgn2b 110 39 the the DT cord-329553-93tbgn2b 110 40 Golgi Golgi NNP cord-329553-93tbgn2b 110 41 apparatus apparatus NN cord-329553-93tbgn2b 110 42 ) ) -RRB- cord-329553-93tbgn2b 111 1 Weinstein Weinstein NNP cord-329553-93tbgn2b 111 2 et et NNP cord-329553-93tbgn2b 111 3 al al NNP cord-329553-93tbgn2b 111 4 . . NNP cord-329553-93tbgn2b 111 5 , , , cord-329553-93tbgn2b 111 6 1987 1987 CD cord-329553-93tbgn2b 111 7 ; ; : cord-329553-93tbgn2b 111 8 our -PRON- PRP$ cord-329553-93tbgn2b 111 9 unpublished unpublished JJ cord-329553-93tbgn2b 111 10 observations observation NNS cord-329553-93tbgn2b 111 11 ) ) -RRB- cord-329553-93tbgn2b 111 12 . . . cord-329553-93tbgn2b 112 1 The the DT cord-329553-93tbgn2b 112 2 NH2-terminal nh2-terminal NN cord-329553-93tbgn2b 112 3 65-residue 65-residue CD cord-329553-93tbgn2b 112 4 sequence sequence NN cord-329553-93tbgn2b 112 5 of of IN cord-329553-93tbgn2b 112 6 rat rat NN cord-329553-93tbgn2b 112 7 ST ST NNP cord-329553-93tbgn2b 112 8 is be VBZ cord-329553-93tbgn2b 112 9 shown show VBN cord-329553-93tbgn2b 112 10 in in IN cord-329553-93tbgn2b 112 11 Fig Fig NNP cord-329553-93tbgn2b 112 12 . . . cord-329553-93tbgn2b 113 1 1 1 LS cord-329553-93tbgn2b 113 2 . . . cord-329553-93tbgn2b 114 1 To to TO cord-329553-93tbgn2b 114 2 determine determine VB cord-329553-93tbgn2b 114 3 the the DT cord-329553-93tbgn2b 114 4 minimum minimum JJ cord-329553-93tbgn2b 114 5 sequence sequence NN cord-329553-93tbgn2b 114 6 ofthe ofthe DT cord-329553-93tbgn2b 114 7 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 114 8 region region NN cord-329553-93tbgn2b 114 9 of of IN cord-329553-93tbgn2b 114 10 ST ST NNP cord-329553-93tbgn2b 114 11 that that WDT cord-329553-93tbgn2b 114 12 is be VBZ cord-329553-93tbgn2b 114 13 sufficient sufficient JJ cord-329553-93tbgn2b 114 14 for for IN cord-329553-93tbgn2b 114 15 membrane membrane NN cord-329553-93tbgn2b 114 16 anchorage anchorage NNP cord-329553-93tbgn2b 114 17 and and CC cord-329553-93tbgn2b 114 18 Golgi Golgi NNP cord-329553-93tbgn2b 114 19 localization localization NN cord-329553-93tbgn2b 114 20 , , , cord-329553-93tbgn2b 114 21 we -PRON- PRP cord-329553-93tbgn2b 114 22 have have VBP cord-329553-93tbgn2b 114 23 constructed construct VBN cord-329553-93tbgn2b 114 24 a a DT cord-329553-93tbgn2b 114 25 series series NN cord-329553-93tbgn2b 114 26 of of IN cord-329553-93tbgn2b 114 27 chimeric chimeric JJ cord-329553-93tbgn2b 114 28 cDNAs cdna NNS cord-329553-93tbgn2b 114 29 which which WDT cord-329553-93tbgn2b 114 30 encode encode VBP cord-329553-93tbgn2b 114 31 different different JJ cord-329553-93tbgn2b 114 32 fusion fusion NN cord-329553-93tbgn2b 114 33 proteins protein NNS cord-329553-93tbgn2b 115 1 ( ( -LRB- cord-329553-93tbgn2b 115 2 Fig Fig NNP cord-329553-93tbgn2b 115 3 . . . cord-329553-93tbgn2b 115 4 1 1 CD cord-329553-93tbgn2b 115 5 B b NN cord-329553-93tbgn2b 115 6 ) ) -RRB- cord-329553-93tbgn2b 115 7 , , , cord-329553-93tbgn2b 115 8 with with IN cord-329553-93tbgn2b 115 9 decreasing decrease VBG cord-329553-93tbgn2b 115 10 lengths length NNS cord-329553-93tbgn2b 115 11 of of IN cord-329553-93tbgn2b 115 12 the the DT cord-329553-93tbgn2b 115 13 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 115 14 ST ST NNP cord-329553-93tbgn2b 115 15 sequence sequence NN cord-329553-93tbgn2b 115 16 fused fuse VBD cord-329553-93tbgn2b 115 17 to to IN cord-329553-93tbgn2b 115 18 the the DT cord-329553-93tbgn2b 115 19 ectodomain ectodomain NN cord-329553-93tbgn2b 115 20 of of IN cord-329553-93tbgn2b 115 21 DPPIV DPPIV NNP cord-329553-93tbgn2b 115 22 . . . cord-329553-93tbgn2b 116 1 The the DT cord-329553-93tbgn2b 116 2 NH2-terminal nh2-terminal NN cord-329553-93tbgn2b 116 3 35residue 35residue JJ cord-329553-93tbgn2b 116 4 sequence sequence NN cord-329553-93tbgn2b 116 5 of of IN cord-329553-93tbgn2b 116 6 DPPIV DPPIV NNP cord-329553-93tbgn2b 116 7 is be VBZ cord-329553-93tbgn2b 116 8 also also RB cord-329553-93tbgn2b 116 9 shown show VBN cord-329553-93tbgn2b 116 10 in in IN cord-329553-93tbgn2b 116 11 Fig Fig NNP cord-329553-93tbgn2b 116 12 . . . cord-329553-93tbgn2b 117 1 1 1 LS cord-329553-93tbgn2b 117 2 A a NN cord-329553-93tbgn2b 117 3 ( ( -LRB- cord-329553-93tbgn2b 117 4 D4 d4 NN cord-329553-93tbgn2b 117 5 ) ) -RRB- cord-329553-93tbgn2b 117 6 . . . cord-329553-93tbgn2b 118 1 Previous previous JJ cord-329553-93tbgn2b 118 2 studies study NNS cord-329553-93tbgn2b 118 3 have have VBP cord-329553-93tbgn2b 118 4 shown show VBN cord-329553-93tbgn2b 118 5 that that IN cord-329553-93tbgn2b 118 6 the the DT cord-329553-93tbgn2b 118 7 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 118 8 34residue 34residue JJ cord-329553-93tbgn2b 118 9 sequence sequence NN cord-329553-93tbgn2b 118 10 of of IN cord-329553-93tbgn2b 118 11 DPPIV DPPIV NNP cord-329553-93tbgn2b 118 12 is be VBZ cord-329553-93tbgn2b 118 13 the the DT cord-329553-93tbgn2b 118 14 signal signal NN cord-329553-93tbgn2b 118 15 / / SYM cord-329553-93tbgn2b 118 16 anchor anchor NN cord-329553-93tbgn2b 118 17 sequence sequence NN cord-329553-93tbgn2b 118 18 ( ( -LRB- cord-329553-93tbgn2b 118 19 Hong Hong NNP cord-329553-93tbgn2b 118 20 and and CC cord-329553-93tbgn2b 118 21 Doyle Doyle NNP cord-329553-93tbgn2b 118 22 , , , cord-329553-93tbgn2b 118 23 1990 1990 CD cord-329553-93tbgn2b 118 24 ) ) -RRB- cord-329553-93tbgn2b 118 25 . . . cord-329553-93tbgn2b 119 1 The the DT cord-329553-93tbgn2b 119 2 first first JJ cord-329553-93tbgn2b 119 3 set set NN cord-329553-93tbgn2b 119 4 of of IN cord-329553-93tbgn2b 119 5 experiments experiment NNS cord-329553-93tbgn2b 119 6 resulted result VBD cord-329553-93tbgn2b 119 7 in in IN cord-329553-93tbgn2b 119 8 the the DT cord-329553-93tbgn2b 119 9 construction construction NN cord-329553-93tbgn2b 119 10 of of IN cord-329553-93tbgn2b 119 11 chimeric chimeric JJ cord-329553-93tbgn2b 119 12 cDNAs cdna NNS cord-329553-93tbgn2b 119 13 encoding encode VBG cord-329553-93tbgn2b 119 14 for for IN cord-329553-93tbgn2b 119 15 fusion fusion NN cord-329553-93tbgn2b 119 16 proteins protein NNS cord-329553-93tbgn2b 119 17 S64D S64D NNP cord-329553-93tbgn2b 119 18 , , , cord-329553-93tbgn2b 119 19 S5 S5 NNP cord-329553-93tbgn2b 119 20 1D 1d CD cord-329553-93tbgn2b 119 21 , , , cord-329553-93tbgn2b 119 22 S46D S46D NNP cord-329553-93tbgn2b 119 23 , , , cord-329553-93tbgn2b 119 24 S40D s40d NN cord-329553-93tbgn2b 119 25 , , , cord-329553-93tbgn2b 119 26 S33D s33d JJ cord-329553-93tbgn2b 119 27 , , , cord-329553-93tbgn2b 119 28 and and CC cord-329553-93tbgn2b 119 29 S27D s27d NN cord-329553-93tbgn2b 119 30 in in IN cord-329553-93tbgn2b 119 31 which which WDT cord-329553-93tbgn2b 119 32 the the DT cord-329553-93tbgn2b 119 33 same same JJ cord-329553-93tbgn2b 119 34 ectodomain ectodomain NN cord-329553-93tbgn2b 119 35 of of IN cord-329553-93tbgn2b 119 36 DPPIV DPPIV NNP cord-329553-93tbgn2b 119 37 ( ( -LRB- cord-329553-93tbgn2b 119 38 from from IN cord-329553-93tbgn2b 119 39 residue residue NN cord-329553-93tbgn2b 119 40 33 33 CD cord-329553-93tbgn2b 119 41 to to IN cord-329553-93tbgn2b 119 42 the the DT cord-329553-93tbgn2b 119 43 COOH COOH NNP cord-329553-93tbgn2b 119 44 - - HYPH cord-329553-93tbgn2b 119 45 terminus terminus NN cord-329553-93tbgn2b 119 46 ) ) -RRB- cord-329553-93tbgn2b 119 47 was be VBD cord-329553-93tbgn2b 119 48 fused fuse VBN cord-329553-93tbgn2b 119 49 to to IN cord-329553-93tbgn2b 119 50 the the DT cord-329553-93tbgn2b 119 51 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 119 52 64- 64- NNP cord-329553-93tbgn2b 119 53 , , , cord-329553-93tbgn2b 119 54 51- 51- CD cord-329553-93tbgn2b 119 55 , , , cord-329553-93tbgn2b 119 56 46- 46- CD cord-329553-93tbgn2b 119 57 , , , cord-329553-93tbgn2b 119 58 40- 40- CD cord-329553-93tbgn2b 119 59 , , , cord-329553-93tbgn2b 119 60 33- 33- CD cord-329553-93tbgn2b 119 61 , , , cord-329553-93tbgn2b 119 62 and and CC cord-329553-93tbgn2b 119 63 27-residue 27-residue CD cord-329553-93tbgn2b 119 64 sequence sequence NN cord-329553-93tbgn2b 119 65 of of IN cord-329553-93tbgn2b 119 66 ST ST NNP cord-329553-93tbgn2b 119 67 ( ( -LRB- cord-329553-93tbgn2b 119 68 Fig Fig NNP cord-329553-93tbgn2b 119 69 . . . cord-329553-93tbgn2b 119 70 1 1 CD cord-329553-93tbgn2b 119 71 B b NN cord-329553-93tbgn2b 119 72 ) ) -RRB- cord-329553-93tbgn2b 119 73 . . . cord-329553-93tbgn2b 120 1 In in IN cord-329553-93tbgn2b 120 2 vitro vitro FW cord-329553-93tbgn2b 120 3 translation translation NN cord-329553-93tbgn2b 120 4 studies study NNS cord-329553-93tbgn2b 120 5 demonstrated demonstrate VBD cord-329553-93tbgn2b 120 6 that that IN cord-329553-93tbgn2b 120 7 all all DT cord-329553-93tbgn2b 120 8 ofthem ofthem NNS cord-329553-93tbgn2b 120 9 , , , cord-329553-93tbgn2b 120 10 except except IN cord-329553-93tbgn2b 120 11 for for IN cord-329553-93tbgn2b 120 12 S27D s27d NN cord-329553-93tbgn2b 120 13 , , , cord-329553-93tbgn2b 120 14 were be VBD cord-329553-93tbgn2b 120 15 efficiently efficiently RB cord-329553-93tbgn2b 120 16 anchored anchor VBN cord-329553-93tbgn2b 120 17 in in IN cord-329553-93tbgn2b 120 18 the the DT cord-329553-93tbgn2b 120 19 membrane membrane NN cord-329553-93tbgn2b 120 20 . . . cord-329553-93tbgn2b 121 1 The the DT cord-329553-93tbgn2b 121 2 results result NNS cord-329553-93tbgn2b 121 3 for for IN cord-329553-93tbgn2b 121 4 S27D s27d NN cord-329553-93tbgn2b 121 5 and and CC cord-329553-93tbgn2b 121 6 S33D s33d NN cord-329553-93tbgn2b 121 7 are be VBP cord-329553-93tbgn2b 121 8 shown show VBN cord-329553-93tbgn2b 121 9 in in IN cord-329553-93tbgn2b 121 10 Fig Fig NNP cord-329553-93tbgn2b 121 11 . . . cord-329553-93tbgn2b 122 1 2 2 LS cord-329553-93tbgn2b 122 2 . . . cord-329553-93tbgn2b 123 1 Results result NNS cord-329553-93tbgn2b 123 2 similar similar JJ cord-329553-93tbgn2b 123 3 to to IN cord-329553-93tbgn2b 123 4 S33D s33d EX cord-329553-93tbgn2b 123 5 were be VBD cord-329553-93tbgn2b 123 6 obtained obtain VBN cord-329553-93tbgn2b 123 7 for for IN cord-329553-93tbgn2b 123 8 S64D s64d NN cord-329553-93tbgn2b 123 9 , , , cord-329553-93tbgn2b 123 10 S51D S51D NNP cord-329553-93tbgn2b 123 11 , , , cord-329553-93tbgn2b 123 12 S46D S46D NNP cord-329553-93tbgn2b 123 13 , , , cord-329553-93tbgn2b 123 14 and and CC cord-329553-93tbgn2b 123 15 S40D s40d NN cord-329553-93tbgn2b 123 16 ( ( -LRB- cord-329553-93tbgn2b 123 17 data datum NNS cord-329553-93tbgn2b 123 18 not not RB cord-329553-93tbgn2b 123 19 shown show VBN cord-329553-93tbgn2b 123 20 ) ) -RRB- cord-329553-93tbgn2b 123 21 . . . cord-329553-93tbgn2b 124 1 These these DT cord-329553-93tbgn2b 124 2 results result NNS cord-329553-93tbgn2b 124 3 demonstrated demonstrate VBD cord-329553-93tbgn2b 124 4 that that IN cord-329553-93tbgn2b 124 5 the the DT cord-329553-93tbgn2b 124 6 NH2-terminal nh2-terminal NN cord-329553-93tbgn2b 124 7 33-but 33-but CD cord-329553-93tbgn2b 124 8 not not RB cord-329553-93tbgn2b 124 9 27-residue 27-residue CD cord-329553-93tbgn2b 124 10 sequence sequence NN cord-329553-93tbgn2b 124 11 of of IN cord-329553-93tbgn2b 124 12 ST ST NNP cord-329553-93tbgn2b 124 13 is be VBZ cord-329553-93tbgn2b 124 14 sufficient sufficient JJ cord-329553-93tbgn2b 124 15 for for IN cord-329553-93tbgn2b 124 16 membrane membrane NNP cord-329553-93tbgn2b 124 17 anchorage anchorage NNP cord-329553-93tbgn2b 124 18 . . . cord-329553-93tbgn2b 125 1 Since since IN cord-329553-93tbgn2b 125 2 S27D s27d NN cord-329553-93tbgn2b 125 3 was be VBD cord-329553-93tbgn2b 125 4 not not RB cord-329553-93tbgn2b 125 5 anchored anchor VBN cord-329553-93tbgn2b 125 6 in in IN cord-329553-93tbgn2b 125 7 the the DT cord-329553-93tbgn2b 125 8 membrane membrane NN cord-329553-93tbgn2b 125 9 , , , cord-329553-93tbgn2b 125 10 it -PRON- PRP cord-329553-93tbgn2b 125 11 was be VBD cord-329553-93tbgn2b 125 12 not not RB cord-329553-93tbgn2b 125 13 pursued pursue VBN cord-329553-93tbgn2b 125 14 further far RBR cord-329553-93tbgn2b 125 15 . . . cord-329553-93tbgn2b 126 1 To to TO cord-329553-93tbgn2b 126 2 examine examine VB cord-329553-93tbgn2b 126 3 the the DT cord-329553-93tbgn2b 126 4 role role NN cord-329553-93tbgn2b 126 5 in in IN cord-329553-93tbgn2b 126 6 Golgi Golgi NNP cord-329553-93tbgn2b 126 7 localization localization NN cord-329553-93tbgn2b 126 8 of of IN cord-329553-93tbgn2b 126 9 the the DT cord-329553-93tbgn2b 126 10 ST ST NNP cord-329553-93tbgn2b 126 11 sequence sequence NN cord-329553-93tbgn2b 126 12 in in IN cord-329553-93tbgn2b 126 13 the the DT cord-329553-93tbgn2b 126 14 remaining remain VBG cord-329553-93tbgn2b 126 15 five five CD cord-329553-93tbgn2b 126 16 fusion fusion NN cord-329553-93tbgn2b 126 17 proteins protein NNS cord-329553-93tbgn2b 126 18 , , , cord-329553-93tbgn2b 126 19 the the DT cord-329553-93tbgn2b 126 20 respective respective JJ cord-329553-93tbgn2b 126 21 chimeric chimeric JJ cord-329553-93tbgn2b 126 22 cDNAs cdna NNS cord-329553-93tbgn2b 126 23 were be VBD cord-329553-93tbgn2b 126 24 inserted insert VBN cord-329553-93tbgn2b 126 25 into into IN cord-329553-93tbgn2b 126 26 the the DT cord-329553-93tbgn2b 126 27 eukaryotic eukaryotic JJ cord-329553-93tbgn2b 126 28 expression expression NN cord-329553-93tbgn2b 126 29 vector vector NN cord-329553-93tbgn2b 126 30 pRSN prsn JJ cord-329553-93tbgn2b 126 31 ( ( -LRB- cord-329553-93tbgn2b 126 32 Low Low NNP cord-329553-93tbgn2b 126 33 et et FW cord-329553-93tbgn2b 126 34 al al NNP cord-329553-93tbgn2b 126 35 . . NNP cord-329553-93tbgn2b 126 36 , , , cord-329553-93tbgn2b 126 37 1991a 1991a CD cord-329553-93tbgn2b 126 38 , , , cord-329553-93tbgn2b 126 39 b b LS cord-329553-93tbgn2b 126 40 ) ) -RRB- cord-329553-93tbgn2b 126 41 and and CC cord-329553-93tbgn2b 126 42 transfected transfecte VBN cord-329553-93tbgn2b 126 43 into into IN cord-329553-93tbgn2b 126 44 MDCK MDCK NNP cord-329553-93tbgn2b 126 45 cells cell NNS cord-329553-93tbgn2b 126 46 . . . cord-329553-93tbgn2b 127 1 Stably stably RB cord-329553-93tbgn2b 127 2 transfected transfecte VBN cord-329553-93tbgn2b 127 3 cells cell NNS cord-329553-93tbgn2b 127 4 were be VBD cord-329553-93tbgn2b 127 5 found find VBN cord-329553-93tbgn2b 127 6 to to TO cord-329553-93tbgn2b 127 7 express express VB cord-329553-93tbgn2b 127 8 the the DT cord-329553-93tbgn2b 127 9 desired desire VBN cord-329553-93tbgn2b 127 10 fusion fusion NN cord-329553-93tbgn2b 127 11 proteins protein NNS cord-329553-93tbgn2b 127 12 . . . cord-329553-93tbgn2b 128 1 Since since IN cord-329553-93tbgn2b 128 2 similar similar JJ cord-329553-93tbgn2b 128 3 results result NNS cord-329553-93tbgn2b 128 4 were be VBD cord-329553-93tbgn2b 128 5 obtained obtain VBN cord-329553-93tbgn2b 128 6 for for IN cord-329553-93tbgn2b 128 7 all all PDT cord-329553-93tbgn2b 128 8 the the DT cord-329553-93tbgn2b 128 9 five five CD cord-329553-93tbgn2b 128 10 fusion fusion NN cord-329553-93tbgn2b 128 11 proteins protein NNS cord-329553-93tbgn2b 128 12 , , , cord-329553-93tbgn2b 128 13 only only RB cord-329553-93tbgn2b 128 14 the the DT cord-329553-93tbgn2b 128 15 results result NNS cord-329553-93tbgn2b 128 16 obtained obtain VBN cord-329553-93tbgn2b 128 17 for for IN cord-329553-93tbgn2b 128 18 fusion fusion NN cord-329553-93tbgn2b 128 19 proteins protein NNS cord-329553-93tbgn2b 129 1 S64D S64D NNP cord-329553-93tbgn2b 129 2 and and CC cord-329553-93tbgn2b 129 3 S33D S33D NNP cord-329553-93tbgn2b 129 4 are be VBP cord-329553-93tbgn2b 129 5 shown show VBN cord-329553-93tbgn2b 129 6 . . . cord-329553-93tbgn2b 130 1 Pulse pulse NN cord-329553-93tbgn2b 130 2 - - HYPH cord-329553-93tbgn2b 130 3 chase chase NN cord-329553-93tbgn2b 130 4 experiments experiment NNS cord-329553-93tbgn2b 130 5 were be VBD cord-329553-93tbgn2b 130 6 performed perform VBN cord-329553-93tbgn2b 130 7 to to TO cord-329553-93tbgn2b 130 8 follow follow VB cord-329553-93tbgn2b 130 9 the the DT cord-329553-93tbgn2b 130 10 intracellular intracellular JJ cord-329553-93tbgn2b 130 11 transport transport NN cord-329553-93tbgn2b 130 12 of of IN cord-329553-93tbgn2b 130 13 S64D s64d NN cord-329553-93tbgn2b 130 14 and and CC cord-329553-93tbgn2b 130 15 S33D s33d NN cord-329553-93tbgn2b 131 1 ( ( -LRB- cord-329553-93tbgn2b 131 2 Fig Fig NNP cord-329553-93tbgn2b 131 3 . . . cord-329553-93tbgn2b 131 4 3 3 CD cord-329553-93tbgn2b 131 5 A a NN cord-329553-93tbgn2b 131 6 ) ) -RRB- cord-329553-93tbgn2b 131 7 . . . cord-329553-93tbgn2b 132 1 As as IN cord-329553-93tbgn2b 132 2 shown show VBN cord-329553-93tbgn2b 132 3 , , , cord-329553-93tbgn2b 132 4 a a DT cord-329553-93tbgn2b 132 5 100-kD 100-kd CD cord-329553-93tbgn2b 132 6 protein protein NN cord-329553-93tbgn2b 132 7 was be VBD cord-329553-93tbgn2b 132 8 specifically specifically RB cord-329553-93tbgn2b 132 9 detected detect VBN cord-329553-93tbgn2b 132 10 in in IN cord-329553-93tbgn2b 132 11 the the DT cord-329553-93tbgn2b 132 12 pulse pulse NN cord-329553-93tbgn2b 132 13 - - HYPH cord-329553-93tbgn2b 132 14 labeled label VBN cord-329553-93tbgn2b 132 15 cells cell NNS cord-329553-93tbgn2b 132 16 expressing express VBG cord-329553-93tbgn2b 132 17 S33D. S33D. NNS cord-329553-93tbgn2b 132 18 As as IN cord-329553-93tbgn2b 132 19 chase chase NN cord-329553-93tbgn2b 132 20 proceeded proceed VBD cord-329553-93tbgn2b 132 21 , , , cord-329553-93tbgn2b 132 22 this this DT cord-329553-93tbgn2b 132 23 1004D 1004d CD cord-329553-93tbgn2b 132 24 form form NN cord-329553-93tbgn2b 132 25 was be VBD cord-329553-93tbgn2b 132 26 converted convert VBN cord-329553-93tbgn2b 132 27 into into IN cord-329553-93tbgn2b 132 28 a a DT cord-329553-93tbgn2b 132 29 110-kD 110-kd CD cord-329553-93tbgn2b 132 30 form form NN cord-329553-93tbgn2b 132 31 . . . cord-329553-93tbgn2b 133 1 This this DT cord-329553-93tbgn2b 133 2 conversion conversion NN cord-329553-93tbgn2b 133 3 was be VBD cord-329553-93tbgn2b 133 4 very very RB cord-329553-93tbgn2b 133 5 efficient efficient JJ cord-329553-93tbgn2b 133 6 , , , cord-329553-93tbgn2b 133 7 as as IN cord-329553-93tbgn2b 133 8 all all DT cord-329553-93tbgn2b 133 9 newly newly RB cord-329553-93tbgn2b 133 10 made make VBN cord-329553-93tbgn2b 133 11 100-kD 100-kd CD cord-329553-93tbgn2b 133 12 molecules molecule NNS cord-329553-93tbgn2b 133 13 were be VBD cord-329553-93tbgn2b 133 14 converted convert VBN cord-329553-93tbgn2b 133 15 into into IN cord-329553-93tbgn2b 133 16 the the DT cord-329553-93tbgn2b 133 17 110-kD 110-kd CD cord-329553-93tbgn2b 133 18 form form NN cord-329553-93tbgn2b 133 19 within within IN cord-329553-93tbgn2b 133 20 90 90 CD cord-329553-93tbgn2b 133 21 min min NN cord-329553-93tbgn2b 133 22 of of IN cord-329553-93tbgn2b 133 23 chase chase NN cord-329553-93tbgn2b 133 24 . . . cord-329553-93tbgn2b 134 1 In in IN cord-329553-93tbgn2b 134 2 cells cell NNS cord-329553-93tbgn2b 134 3 expressing express VBG cord-329553-93tbgn2b 134 4 S64D S64D NNP cord-329553-93tbgn2b 134 5 , , , cord-329553-93tbgn2b 134 6 a a DT cord-329553-93tbgn2b 134 7 105-kD 105-kd CD cord-329553-93tbgn2b 134 8 form form NN cord-329553-93tbgn2b 134 9 was be VBD cord-329553-93tbgn2b 134 10 initially initially RB cord-329553-93tbgn2b 134 11 detected detect VBN cord-329553-93tbgn2b 134 12 in in IN cord-329553-93tbgn2b 134 13 pulse pulse NN cord-329553-93tbgn2b 134 14 - - HYPH cord-329553-93tbgn2b 134 15 labeled label VBN cord-329553-93tbgn2b 134 16 cells cell NNS cord-329553-93tbgn2b 134 17 and and CC cord-329553-93tbgn2b 134 18 this this DT cord-329553-93tbgn2b 134 19 form form NN cord-329553-93tbgn2b 134 20 was be VBD cord-329553-93tbgn2b 134 21 converted convert VBN cord-329553-93tbgn2b 134 22 into into IN cord-329553-93tbgn2b 134 23 a a DT cord-329553-93tbgn2b 134 24 115-kD 115-kd CD cord-329553-93tbgn2b 134 25 form form NN cord-329553-93tbgn2b 134 26 during during IN cord-329553-93tbgn2b 134 27 the the DT cord-329553-93tbgn2b 134 28 chase chase NN cord-329553-93tbgn2b 134 29 period period NN cord-329553-93tbgn2b 134 30 . . . cord-329553-93tbgn2b 135 1 A a DT cord-329553-93tbgn2b 135 2 majority majority NN cord-329553-93tbgn2b 135 3 of of IN cord-329553-93tbgn2b 135 4 the the DT cord-329553-93tbgn2b 135 5 105-kD 105-kd CD cord-329553-93tbgn2b 135 6 form form NN cord-329553-93tbgn2b 135 7 was be VBD cord-329553-93tbgn2b 135 8 converted convert VBN cord-329553-93tbgn2b 135 9 into into IN cord-329553-93tbgn2b 135 10 the the DT cord-329553-93tbgn2b 135 11 115-kD 115-kd CD cord-329553-93tbgn2b 135 12 form form NN cord-329553-93tbgn2b 135 13 after after IN cord-329553-93tbgn2b 135 14 90 90 CD cord-329553-93tbgn2b 135 15 min min NN cord-329553-93tbgn2b 135 16 of of IN cord-329553-93tbgn2b 135 17 chase chase NN cord-329553-93tbgn2b 135 18 , , , cord-329553-93tbgn2b 135 19 although although IN cord-329553-93tbgn2b 135 20 a a DT cord-329553-93tbgn2b 135 21 small small JJ cord-329553-93tbgn2b 135 22 amount amount NN cord-329553-93tbgn2b 135 23 of of IN cord-329553-93tbgn2b 135 24 the the DT cord-329553-93tbgn2b 135 25 105-kD 105-kd CD cord-329553-93tbgn2b 135 26 form form NN cord-329553-93tbgn2b 135 27 was be VBD cord-329553-93tbgn2b 135 28 still still RB cord-329553-93tbgn2b 135 29 observed observe VBN cord-329553-93tbgn2b 135 30 . . . cord-329553-93tbgn2b 136 1 Endo Endo NNP cord-329553-93tbgn2b 136 2 H H NNP cord-329553-93tbgn2b 136 3 treatment treatment NN cord-329553-93tbgn2b 136 4 ofimmunoprecipitated ofimmunoprecipitate VBN cord-329553-93tbgn2b 136 5 proteins protein NNS cord-329553-93tbgn2b 137 1 ( ( -LRB- cord-329553-93tbgn2b 137 2 Fig Fig NNP cord-329553-93tbgn2b 137 3 . . . cord-329553-93tbgn2b 137 4 3 3 CD cord-329553-93tbgn2b 137 5 B b NN cord-329553-93tbgn2b 137 6 ) ) -RRB- cord-329553-93tbgn2b 137 7 showed show VBD cord-329553-93tbgn2b 137 8 that that IN cord-329553-93tbgn2b 137 9 the the DT cord-329553-93tbgn2b 137 10 100-kD 100-kd CD cord-329553-93tbgn2b 137 11 form form NN cord-329553-93tbgn2b 137 12 ofS33D ofS33D NNP cord-329553-93tbgn2b 137 13 and and CC cord-329553-93tbgn2b 137 14 the the DT cord-329553-93tbgn2b 137 15 105-kD 105-kd CD cord-329553-93tbgn2b 137 16 formofS64Dwere formofS64Dwere NNP cord-329553-93tbgn2b 137 17 mainly mainly RB cord-329553-93tbgn2b 137 18 converted convert VBN cord-329553-93tbgn2b 137 19 into into IN cord-329553-93tbgn2b 137 20 80-and 80-and CD cord-329553-93tbgn2b 137 21 85-kD 85-kd CD cord-329553-93tbgn2b 137 22 polypeptides polypeptide NNS cord-329553-93tbgn2b 137 23 , , , cord-329553-93tbgn2b 137 24 respectively respectively RB cord-329553-93tbgn2b 137 25 , , , cord-329553-93tbgn2b 137 26 which which WDT cord-329553-93tbgn2b 137 27 were be VBD cord-329553-93tbgn2b 137 28 of of IN cord-329553-93tbgn2b 137 29 the the DT cord-329553-93tbgn2b 137 30 same same JJ cord-329553-93tbgn2b 137 31 size size NN cord-329553-93tbgn2b 137 32 as as IN cord-329553-93tbgn2b 137 33 the the DT cord-329553-93tbgn2b 137 34 respective respective JJ cord-329553-93tbgn2b 137 35 polypeptides polypeptide NNS cord-329553-93tbgn2b 137 36 without without IN cord-329553-93tbgn2b 137 37 any any DT cord-329553-93tbgn2b 137 38 glycosylation glycosylation NN cord-329553-93tbgn2b 137 39 ( ( -LRB- cord-329553-93tbgn2b 137 40 produced produce VBN cord-329553-93tbgn2b 137 41 by by IN cord-329553-93tbgn2b 137 42 in in FW cord-329553-93tbgn2b 137 43 vitro vitro FW cord-329553-93tbgn2b 137 44 translation translation NN cord-329553-93tbgn2b 137 45 without without IN cord-329553-93tbgn2b 137 46 microsomes microsomes NNP cord-329553-93tbgn2b 137 47 ) ) -RRB- cord-329553-93tbgn2b 137 48 . . . cord-329553-93tbgn2b 138 1 This this DT cord-329553-93tbgn2b 138 2 result result NN cord-329553-93tbgn2b 138 3 suggests suggest VBZ cord-329553-93tbgn2b 138 4 that that IN cord-329553-93tbgn2b 138 5 all all PDT cord-329553-93tbgn2b 138 6 the the DT cord-329553-93tbgn2b 138 7 N n NN cord-329553-93tbgn2b 138 8 - - HYPH cord-329553-93tbgn2b 138 9 linked link VBN cord-329553-93tbgn2b 138 10 glycans glycan NNS cord-329553-93tbgn2b 138 11 ( ( -LRB- cord-329553-93tbgn2b 138 12 about about RB cord-329553-93tbgn2b 138 13 7 7 CD cord-329553-93tbgn2b 138 14 - - SYM cord-329553-93tbgn2b 138 15 8 8 CD cord-329553-93tbgn2b 138 16 N n CD cord-329553-93tbgn2b 138 17 - - HYPH cord-329553-93tbgn2b 138 18 linked link VBN cord-329553-93tbgn2b 138 19 glycans glycan NNS cord-329553-93tbgn2b 138 20 , , , cord-329553-93tbgn2b 138 21 but but CC cord-329553-93tbgn2b 138 22 no no DT cord-329553-93tbgn2b 138 23 detectable detectable JJ cord-329553-93tbgn2b 138 24 0-linked 0-linked CD cord-329553-93tbgn2b 138 25 glycans glycan NNS cord-329553-93tbgn2b 138 26 , , , cord-329553-93tbgn2b 138 27 were be VBD cord-329553-93tbgn2b 138 28 present present JJ cord-329553-93tbgn2b 138 29 in in IN cord-329553-93tbgn2b 138 30 the the DT cord-329553-93tbgn2b 138 31 ectodomain ectodomain NN cord-329553-93tbgn2b 138 32 of of IN cord-329553-93tbgn2b 138 33 DPPIV DPPIV NNP cord-329553-93tbgn2b 139 1 [ [ -LRB- cord-329553-93tbgn2b 139 2 Hong Hong NNP cord-329553-93tbgn2b 139 3 and and CC cord-329553-93tbgn2b 139 4 Doyle Doyle NNP cord-329553-93tbgn2b 139 5 , , , cord-329553-93tbgn2b 139 6 1988 1988 CD cord-329553-93tbgn2b 139 7 ; ; : cord-329553-93tbgn2b 139 8 Hong Hong NNP cord-329553-93tbgn2b 139 9 et et NNP cord-329553-93tbgn2b 139 10 al al NNP cord-329553-93tbgn2b 139 11 . . NNP cord-329553-93tbgn2b 139 12 , , , cord-329553-93tbgn2b 139 13 1989 1989 CD cord-329553-93tbgn2b 139 14 ] ] -RRB- cord-329553-93tbgn2b 140 1 ) ) -RRB- cord-329553-93tbgn2b 140 2 attached attach VBN cord-329553-93tbgn2b 140 3 to to IN cord-329553-93tbgn2b 140 4 the the DT cord-329553-93tbgn2b 140 5 100-kD 100-kd CD cord-329553-93tbgn2b 140 6 form form NN cord-329553-93tbgn2b 140 7 of of IN cord-329553-93tbgn2b 140 8 S33D s33d NN cord-329553-93tbgn2b 140 9 and and CC cord-329553-93tbgn2b 140 10 the the DT cord-329553-93tbgn2b 140 11 105-kD 105-kd CD cord-329553-93tbgn2b 140 12 form form NN cord-329553-93tbgn2b 140 13 of of IN cord-329553-93tbgn2b 140 14 S64D s64d NN cord-329553-93tbgn2b 140 15 were be VBD cord-329553-93tbgn2b 140 16 essentially essentially RB cord-329553-93tbgn2b 140 17 sensitive sensitive JJ cord-329553-93tbgn2b 140 18 to to IN cord-329553-93tbgn2b 140 19 endo endo FW cord-329553-93tbgn2b 140 20 H h NN cord-329553-93tbgn2b 140 21 and and CC cord-329553-93tbgn2b 140 22 that that IN cord-329553-93tbgn2b 140 23 they -PRON- PRP cord-329553-93tbgn2b 140 24 represent represent VBP cord-329553-93tbgn2b 140 25 the the DT cord-329553-93tbgn2b 140 26 newly newly RB cord-329553-93tbgn2b 140 27 made make VBN cord-329553-93tbgn2b 140 28 proteins protein NNS cord-329553-93tbgn2b 140 29 in in IN cord-329553-93tbgn2b 140 30 the the DT cord-329553-93tbgn2b 140 31 ER ER NNP cord-329553-93tbgn2b 140 32 bearing bear VBG cord-329553-93tbgn2b 140 33 N N NNP cord-329553-93tbgn2b 140 34 - - HYPH cord-329553-93tbgn2b 140 35 linked link VBN cord-329553-93tbgn2b 140 36 glycans glycan NNS cord-329553-93tbgn2b 140 37 of of IN cord-329553-93tbgn2b 140 38 the the DT cord-329553-93tbgn2b 140 39 highmannose highmannose JJ cord-329553-93tbgn2b 140 40 type type NN cord-329553-93tbgn2b 140 41 . . . cord-329553-93tbgn2b 141 1 The the DT cord-329553-93tbgn2b 141 2 size size NN cord-329553-93tbgn2b 141 3 of of IN cord-329553-93tbgn2b 141 4 the the DT cord-329553-93tbgn2b 141 5 110-kD 110-kd CD cord-329553-93tbgn2b 141 6 form form NN cord-329553-93tbgn2b 141 7 of of IN cord-329553-93tbgn2b 141 8 S33D s33d NN cord-329553-93tbgn2b 141 9 was be VBD cord-329553-93tbgn2b 141 10 reduced reduce VBN cord-329553-93tbgn2b 141 11 by by IN cord-329553-93tbgn2b 141 12 4 4 CD cord-329553-93tbgn2b 141 13 - - SYM cord-329553-93tbgn2b 141 14 5-kD 5-kd CD cord-329553-93tbgn2b 141 15 after after IN cord-329553-93tbgn2b 141 16 endo endo NNP cord-329553-93tbgn2b 141 17 H H NNP cord-329553-93tbgn2b 141 18 treatment treatment NN cord-329553-93tbgn2b 141 19 , , , cord-329553-93tbgn2b 141 20 suggesting suggest VBG cord-329553-93tbgn2b 141 21 that that IN cord-329553-93tbgn2b 141 22 about about RB cord-329553-93tbgn2b 141 23 two two CD cord-329553-93tbgn2b 141 24 of of IN cord-329553-93tbgn2b 141 25 the the DT cord-329553-93tbgn2b 141 26 N n NN cord-329553-93tbgn2b 141 27 - - HYPH cord-329553-93tbgn2b 141 28 linked link VBN cord-329553-93tbgn2b 141 29 glycans glycan NNS cord-329553-93tbgn2b 141 30 in in IN cord-329553-93tbgn2b 141 31 the the DT cord-329553-93tbgn2b 141 32 polypeptide polypeptide NN cord-329553-93tbgn2b 141 33 were be VBD cord-329553-93tbgn2b 141 34 of of IN cord-329553-93tbgn2b 141 35 the the DT cord-329553-93tbgn2b 141 36 high high JJ cord-329553-93tbgn2b 141 37 - - HYPH cord-329553-93tbgn2b 141 38 mannose mannose NN cord-329553-93tbgn2b 141 39 type type NN cord-329553-93tbgn2b 141 40 while while IN cord-329553-93tbgn2b 141 41 the the DT cord-329553-93tbgn2b 141 42 remaining remain VBG cord-329553-93tbgn2b 141 43 N n NN cord-329553-93tbgn2b 141 44 - - HYPH cord-329553-93tbgn2b 141 45 linked link VBN cord-329553-93tbgn2b 141 46 glycans glycan NNS cord-329553-93tbgn2b 141 47 ( ( -LRB- cord-329553-93tbgn2b 141 48 N5 N5 NNP cord-329553-93tbgn2b 141 49 - - HYPH cord-329553-93tbgn2b 141 50 6 6 CD cord-329553-93tbgn2b 141 51 ) ) -RRB- cord-329553-93tbgn2b 141 52 were be VBD cord-329553-93tbgn2b 141 53 resistant resistant JJ cord-329553-93tbgn2b 141 54 to to IN cord-329553-93tbgn2b 141 55 endo endo NN cord-329553-93tbgn2b 141 56 H. h. NN cord-329553-93tbgn2b 142 1 For for IN cord-329553-93tbgn2b 142 2 the the DT cord-329553-93tbgn2b 142 3 MAD mad JJ cord-329553-93tbgn2b 142 4 form form NN cord-329553-93tbgn2b 142 5 Residue residue NN cord-329553-93tbgn2b 142 6 numbers number NNS cord-329553-93tbgn2b 142 7 from from IN cord-329553-93tbgn2b 142 8 the the DT cord-329553-93tbgn2b 142 9 NH2 NH2 NNP cord-329553-93tbgn2b 142 10 terminus terminus NN cord-329553-93tbgn2b 142 11 are be VBP cord-329553-93tbgn2b 142 12 indicated indicate VBN cord-329553-93tbgn2b 142 13 above above IN cord-329553-93tbgn2b 142 14 the the DT cord-329553-93tbgn2b 142 15 sequence sequence NN cord-329553-93tbgn2b 142 16 . . . cord-329553-93tbgn2b 143 1 ( ( -LRB- cord-329553-93tbgn2b 143 2 B b NN cord-329553-93tbgn2b 143 3 ) ) -RRB- cord-329553-93tbgn2b 143 4 Summary summary NN cord-329553-93tbgn2b 143 5 of of IN cord-329553-93tbgn2b 143 6 fusion fusion NN cord-329553-93tbgn2b 143 7 proteins protein NNS cord-329553-93tbgn2b 143 8 used use VBN cord-329553-93tbgn2b 143 9 in in IN cord-329553-93tbgn2b 143 10 this this DT cord-329553-93tbgn2b 143 11 study study NN cord-329553-93tbgn2b 143 12 and and CC cord-329553-93tbgn2b 143 13 their -PRON- PRP$ cord-329553-93tbgn2b 143 14 properties property NNS cord-329553-93tbgn2b 143 15 in in IN cord-329553-93tbgn2b 143 16 membrane membrane NN cord-329553-93tbgn2b 143 17 anchorage anchorage NNP cord-329553-93tbgn2b 143 18 and and CC cord-329553-93tbgn2b 143 19 cellular cellular JJ cord-329553-93tbgn2b 143 20 localization localization NN cord-329553-93tbgn2b 143 21 . . . cord-329553-93tbgn2b 144 1 Residue residue NN cord-329553-93tbgn2b 144 2 numbers number NNS cord-329553-93tbgn2b 144 3 corresponding correspond VBG cord-329553-93tbgn2b 144 4 to to IN cord-329553-93tbgn2b 144 5 ST ST NNP cord-329553-93tbgn2b 144 6 are be VBP cord-329553-93tbgn2b 144 7 indicated indicate VBN cord-329553-93tbgn2b 144 8 above above IN cord-329553-93tbgn2b 144 9 while while IN cord-329553-93tbgn2b 144 10 those those DT cord-329553-93tbgn2b 144 11 to to IN cord-329553-93tbgn2b 144 12 D4 d4 NN cord-329553-93tbgn2b 144 13 are be VBP cord-329553-93tbgn2b 144 14 indicated indicate VBN cord-329553-93tbgn2b 144 15 below below IN cord-329553-93tbgn2b 144 16 each each DT cord-329553-93tbgn2b 144 17 fusion fusion NN cord-329553-93tbgn2b 144 18 protein protein NN cord-329553-93tbgn2b 144 19 . . . cord-329553-93tbgn2b 145 1 For for IN cord-329553-93tbgn2b 145 2 fusion fusion NN cord-329553-93tbgn2b 145 3 proteins protein NNS cord-329553-93tbgn2b 145 4 , , , cord-329553-93tbgn2b 145 5 they -PRON- PRP cord-329553-93tbgn2b 145 6 were be VBD cord-329553-93tbgn2b 145 7 named name VBN cord-329553-93tbgn2b 145 8 SxD SxD NNP cord-329553-93tbgn2b 145 9 ( ( -LRB- cord-329553-93tbgn2b 145 10 in in IN cord-329553-93tbgn2b 145 11 which which WDT cord-329553-93tbgn2b 145 12 the the DT cord-329553-93tbgn2b 145 13 NH2-terminal NH2-terminal NNP cord-329553-93tbgn2b 145 14 X x NN cord-329553-93tbgn2b 145 15 - - NN cord-329553-93tbgn2b 145 16 residue residue JJ cord-329553-93tbgn2b 145 17 sequence sequence NN cord-329553-93tbgn2b 145 18 of of IN cord-329553-93tbgn2b 145 19 ST ST NNP cord-329553-93tbgn2b 145 20 was be VBD cord-329553-93tbgn2b 145 21 fused fuse VBN cord-329553-93tbgn2b 145 22 to to IN cord-329553-93tbgn2b 145 23 the the DT cord-329553-93tbgn2b 145 24 ectodomain ectodomain NN cord-329553-93tbgn2b 145 25 of of IN cord-329553-93tbgn2b 145 26 DPPIV DPPIV NNP cord-329553-93tbgn2b 145 27 ) ) -RRB- cord-329553-93tbgn2b 145 28 or or CC cord-329553-93tbgn2b 146 1 Sx Sx NNP cord-329553-93tbgn2b 146 2 - - HYPH cord-329553-93tbgn2b 146 3 yD yD NNP cord-329553-93tbgn2b 146 4 ( ( -LRB- cord-329553-93tbgn2b 146 5 in in IN cord-329553-93tbgn2b 146 6 which which WDT cord-329553-93tbgn2b 146 7 the the DT cord-329553-93tbgn2b 146 8 ST ST NNP cord-329553-93tbgn2b 146 9 sequence sequence NN cord-329553-93tbgn2b 146 10 from from IN cord-329553-93tbgn2b 146 11 residue residue NN cord-329553-93tbgn2b 146 12 x x NN cord-329553-93tbgn2b 146 13 to to IN cord-329553-93tbgn2b 146 14 y y NNP cord-329553-93tbgn2b 146 15 was be VBD cord-329553-93tbgn2b 146 16 used use VBN cord-329553-93tbgn2b 146 17 to to TO cord-329553-93tbgn2b 146 18 replace replace VB cord-329553-93tbgn2b 146 19 the the DT cord-329553-93tbgn2b 146 20 corresponding correspond VBG cord-329553-93tbgn2b 146 21 region region NN cord-329553-93tbgn2b 146 22 of of IN cord-329553-93tbgn2b 146 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 146 24 ) ) -RRB- cord-329553-93tbgn2b 146 25 . . . cord-329553-93tbgn2b 147 1 For for IN cord-329553-93tbgn2b 147 2 membrane membrane NN cord-329553-93tbgn2b 147 3 anchorage anchorage NNP cord-329553-93tbgn2b 147 4 , , , cord-329553-93tbgn2b 147 5 + + CC cord-329553-93tbgn2b 147 6 and and CC cord-329553-93tbgn2b 147 7 -indicates -indicate VBZ cord-329553-93tbgn2b 147 8 that that IN cord-329553-93tbgn2b 147 9 the the DT cord-329553-93tbgn2b 147 10 majority majority NN cord-329553-93tbgn2b 147 11 of of IN cord-329553-93tbgn2b 147 12 the the DT cord-329553-93tbgn2b 147 13 protein protein NN cord-329553-93tbgn2b 147 14 is be VBZ cord-329553-93tbgn2b 147 15 membrane membrane NN cord-329553-93tbgn2b 147 16 anchored anchor VBN cord-329553-93tbgn2b 147 17 or or CC cord-329553-93tbgn2b 147 18 not not RB cord-329553-93tbgn2b 147 19 , , , cord-329553-93tbgn2b 147 20 respectively respectively RB cord-329553-93tbgn2b 147 21 , , , cord-329553-93tbgn2b 147 22 as as IN cord-329553-93tbgn2b 147 23 assessed assess VBN cord-329553-93tbgn2b 147 24 by by IN cord-329553-93tbgn2b 147 25 in in IN cord-329553-93tbgn2b 147 26 vitro vitro FW cord-329553-93tbgn2b 147 27 translation translation NN cord-329553-93tbgn2b 147 28 experiments experiment NNS cord-329553-93tbgn2b 147 29 . . . cord-329553-93tbgn2b 148 1 For for IN cord-329553-93tbgn2b 148 2 cellular cellular JJ cord-329553-93tbgn2b 148 3 localization localization NN cord-329553-93tbgn2b 148 4 , , , cord-329553-93tbgn2b 148 5 Sand Sand NNP cord-329553-93tbgn2b 148 6 Grepresent Grepresent NNP cord-329553-93tbgn2b 148 7 surface surface NN cord-329553-93tbgn2b 148 8 or or CC cord-329553-93tbgn2b 148 9 Golgi Golgi NNP cord-329553-93tbgn2b 148 10 localization localization NN cord-329553-93tbgn2b 148 11 , , , cord-329553-93tbgn2b 148 12 respectively respectively RB cord-329553-93tbgn2b 148 13 , , , cord-329553-93tbgn2b 148 14 as as IN cord-329553-93tbgn2b 148 15 examined examine VBN cord-329553-93tbgn2b 148 16 by by IN cord-329553-93tbgn2b 148 17 indirect indirect JJ cord-329553-93tbgn2b 148 18 immunofluorescence immunofluorescence NN cord-329553-93tbgn2b 148 19 microscopy microscopy NN cord-329553-93tbgn2b 148 20 , , , cord-329553-93tbgn2b 148 21 while while IN cord-329553-93tbgn2b 148 22 nd nd NNP cord-329553-93tbgn2b 148 23 indicates indicate VBZ cord-329553-93tbgn2b 148 24 " " `` cord-329553-93tbgn2b 148 25 not not RB cord-329553-93tbgn2b 148 26 determined determine VBN cord-329553-93tbgn2b 148 27 " " '' cord-329553-93tbgn2b 148 28 because because IN cord-329553-93tbgn2b 148 29 of of IN cord-329553-93tbgn2b 148 30 theirnonanchorage theirnonanchorage NN cord-329553-93tbgn2b 148 31 in in IN cord-329553-93tbgn2b 148 32 the the DT cord-329553-93tbgn2b 148 33 membrane membrane NN cord-329553-93tbgn2b 148 34 . . . cord-329553-93tbgn2b 149 1 Figure figure NN cord-329553-93tbgn2b 149 2 2 2 CD cord-329553-93tbgn2b 149 3 . . . cord-329553-93tbgn2b 150 1 Membrane Membrane NNP cord-329553-93tbgn2b 150 2 anchorage anchorage NN cord-329553-93tbgn2b 150 3 of of IN cord-329553-93tbgn2b 150 4 selected select VBN cord-329553-93tbgn2b 150 5 fusion fusion NN cord-329553-93tbgn2b 150 6 proteins protein NNS cord-329553-93tbgn2b 150 7 . . . cord-329553-93tbgn2b 151 1 The the DT cord-329553-93tbgn2b 151 2 mRNA mrna NN cord-329553-93tbgn2b 151 3 for for IN cord-329553-93tbgn2b 151 4 the the DT cord-329553-93tbgn2b 151 5 fusion fusion NN cord-329553-93tbgn2b 151 6 protein protein NN cord-329553-93tbgn2b 151 7 was be VBD cord-329553-93tbgn2b 151 8 translated translate VBN cord-329553-93tbgn2b 151 9 in in IN cord-329553-93tbgn2b 151 10 rabbit rabbit NN cord-329553-93tbgn2b 151 11 reticulocyte reticulocyte NNP cord-329553-93tbgn2b 151 12 lysate lysate NN cord-329553-93tbgn2b 151 13 supplemented supplement VBN cord-329553-93tbgn2b 151 14 with with IN cord-329553-93tbgn2b 151 15 microsomes microsome NNS cord-329553-93tbgn2b 151 16 as as IN cord-329553-93tbgn2b 151 17 described describe VBN cord-329553-93tbgn2b 151 18 previously previously RB cord-329553-93tbgn2b 151 19 Doyle Doyle NNP cord-329553-93tbgn2b 151 20 , , , cord-329553-93tbgn2b 151 21 1988 1988 CD cord-329553-93tbgn2b 151 22 , , , cord-329553-93tbgn2b 151 23 1990 1990 CD cord-329553-93tbgn2b 151 24 ) ) -RRB- cord-329553-93tbgn2b 151 25 . . . cord-329553-93tbgn2b 152 1 10 10 CD cord-329553-93tbgn2b 152 2 pl pl NNS cord-329553-93tbgn2b 152 3 of of IN cord-329553-93tbgn2b 152 4 total total JJ cord-329553-93tbgn2b 152 5 translation translation NN cord-329553-93tbgn2b 152 6 reaction reaction NN cord-329553-93tbgn2b 152 7 was be VBD cord-329553-93tbgn2b 152 8 diluted dilute VBN cord-329553-93tbgn2b 152 9 with with IN cord-329553-93tbgn2b 152 10 100,u1 100,u1 CD cord-329553-93tbgn2b 152 11 of of IN cord-329553-93tbgn2b 152 12 sodium sodium NN cord-329553-93tbgn2b 152 13 bicarbonate bicarbonate NN cord-329553-93tbgn2b 152 14 , , , cord-329553-93tbgn2b 152 15 pH ph NN cord-329553-93tbgn2b 152 16 11 11 CD cord-329553-93tbgn2b 152 17 .0 .0 CD cord-329553-93tbgn2b 152 18 , , , cord-329553-93tbgn2b 152 19 and and CC cord-329553-93tbgn2b 152 20 the the DT cord-329553-93tbgn2b 152 21 membrane membrane NN cord-329553-93tbgn2b 152 22 - - HYPH cord-329553-93tbgn2b 152 23 associated associate VBN cord-329553-93tbgn2b 152 24 proteins protein NNS cord-329553-93tbgn2b 152 25 ( ( -LRB- cord-329553-93tbgn2b 152 26 P p NN cord-329553-93tbgn2b 152 27 ) ) -RRB- cord-329553-93tbgn2b 152 28 were be VBD cord-329553-93tbgn2b 152 29 separated separate VBN cord-329553-93tbgn2b 152 30 from from IN cord-329553-93tbgn2b 152 31 those those DT cord-329553-93tbgn2b 152 32 extractable extractable JJ cord-329553-93tbgn2b 152 33 by by IN cord-329553-93tbgn2b 152 34 this this DT cord-329553-93tbgn2b 152 35 treatment treatment NN cord-329553-93tbgn2b 152 36 ( ( -LRB- cord-329553-93tbgn2b 152 37 S S NNP cord-329553-93tbgn2b 152 38 ) ) -RRB- cord-329553-93tbgn2b 152 39 . . . cord-329553-93tbgn2b 153 1 Samples sample NNS cord-329553-93tbgn2b 153 2 equivalent equivalent JJ cord-329553-93tbgn2b 153 3 to to IN cord-329553-93tbgn2b 153 4 4 4 CD cord-329553-93tbgn2b 153 5 gl gl NNS cord-329553-93tbgn2b 153 6 of of IN cord-329553-93tbgn2b 153 7 original original JJ cord-329553-93tbgn2b 153 8 translation translation NN cord-329553-93tbgn2b 153 9 were be VBD cord-329553-93tbgn2b 153 10 analyzed analyze VBN cord-329553-93tbgn2b 153 11 forboth forboth NN cord-329553-93tbgn2b 153 12 P p NN cord-329553-93tbgn2b 153 13 and and CC cord-329553-93tbgn2b 153 14 S S NNP cord-329553-93tbgn2b 153 15 fractions fraction NNS cord-329553-93tbgn2b 153 16 . . . cord-329553-93tbgn2b 154 1 The the DT cord-329553-93tbgn2b 154 2 80-kD 80-kd CD cord-329553-93tbgn2b 154 3 polypeptide polypeptide NN cord-329553-93tbgn2b 154 4 is be VBZ cord-329553-93tbgn2b 154 5 the the DT cord-329553-93tbgn2b 154 6 untranslocated untranslocated JJ cord-329553-93tbgn2b 154 7 form form NN cord-329553-93tbgn2b 154 8 , , , cord-329553-93tbgn2b 154 9 while while IN cord-329553-93tbgn2b 154 10 the the DT cord-329553-93tbgn2b 154 11 100-kD 100-kd CD cord-329553-93tbgn2b 154 12 polypeptide polypeptide NN cord-329553-93tbgn2b 154 13 is be VBZ cord-329553-93tbgn2b 154 14 the the DT cord-329553-93tbgn2b 154 15 translocated translocate VBN cord-329553-93tbgn2b 154 16 N N NNP cord-329553-93tbgn2b 154 17 - - HYPH cord-329553-93tbgn2b 154 18 glycosylated glycosylate VBN cord-329553-93tbgn2b 154 19 form form NN cord-329553-93tbgn2b 154 20 for for IN cord-329553-93tbgn2b 154 21 the the DT cord-329553-93tbgn2b 154 22 respective respective JJ cord-329553-93tbgn2b 154 23 fusion fusion NN cord-329553-93tbgn2b 154 24 proteins protein NNS cord-329553-93tbgn2b 154 25 . . . cord-329553-93tbgn2b 155 1 of of IN cord-329553-93tbgn2b 155 2 S64D S64D NNP cord-329553-93tbgn2b 155 3 , , , cord-329553-93tbgn2b 155 4 a a DT cord-329553-93tbgn2b 155 5 2 2 CD cord-329553-93tbgn2b 155 6 - - SYM cord-329553-93tbgn2b 155 7 3 3 CD cord-329553-93tbgn2b 155 8 kD kd JJ cord-329553-93tbgn2b 155 9 size size NN cord-329553-93tbgn2b 155 10 decrease decrease NN cord-329553-93tbgn2b 155 11 was be VBD cord-329553-93tbgn2b 155 12 observed observe VBN cord-329553-93tbgn2b 155 13 after after IN cord-329553-93tbgn2b 155 14 endo endo NNP cord-329553-93tbgn2b 155 15 H H NNP cord-329553-93tbgn2b 155 16 treatment treatment NN cord-329553-93tbgn2b 155 17 , , , cord-329553-93tbgn2b 155 18 suggesting suggest VBG cord-329553-93tbgn2b 155 19 that that IN cord-329553-93tbgn2b 155 20 one one CD cord-329553-93tbgn2b 155 21 of of IN cord-329553-93tbgn2b 155 22 the the DT cord-329553-93tbgn2b 155 23 N n NN cord-329553-93tbgn2b 155 24 - - HYPH cord-329553-93tbgn2b 155 25 linked link VBN cord-329553-93tbgn2b 155 26 glycans glycan NNS cord-329553-93tbgn2b 155 27 was be VBD cord-329553-93tbgn2b 155 28 a a DT cord-329553-93tbgn2b 155 29 high high JJ cord-329553-93tbgn2b 155 30 - - HYPH cord-329553-93tbgn2b 155 31 mannose mannose NN cord-329553-93tbgn2b 155 32 type type NN cord-329553-93tbgn2b 155 33 while while IN cord-329553-93tbgn2b 155 34 the the DT cord-329553-93tbgn2b 155 35 rest rest NN cord-329553-93tbgn2b 155 36 ( ( -LRB- cord-329553-93tbgn2b 155 37 -6 -6 NNP cord-329553-93tbgn2b 155 38 - - SYM cord-329553-93tbgn2b 155 39 7 7 CD cord-329553-93tbgn2b 155 40 ) ) -RRB- cord-329553-93tbgn2b 155 41 of of IN cord-329553-93tbgn2b 155 42 the the DT cord-329553-93tbgn2b 155 43 N n NN cord-329553-93tbgn2b 155 44 - - HYPH cord-329553-93tbgn2b 155 45 linked link VBN cord-329553-93tbgn2b 155 46 glycans glycan NNS cord-329553-93tbgn2b 155 47 were be VBD cord-329553-93tbgn2b 155 48 endo endo NNP cord-329553-93tbgn2b 155 49 H H NNP cord-329553-93tbgn2b 155 50 resistant resistant JJ cord-329553-93tbgn2b 155 51 . . . cord-329553-93tbgn2b 156 1 These these DT cord-329553-93tbgn2b 156 2 results result NNS cord-329553-93tbgn2b 156 3 demonstrate demonstrate VBP cord-329553-93tbgn2b 156 4 that that IN cord-329553-93tbgn2b 156 5 the the DT cord-329553-93tbgn2b 156 6 majority majority NN cord-329553-93tbgn2b 156 7 of of IN cord-329553-93tbgn2b 156 8 the the DT cord-329553-93tbgn2b 156 9 N N NNP cord-329553-93tbgn2b 156 10 - - HYPH cord-329553-93tbgn2b 156 11 linked link VBN cord-329553-93tbgn2b 156 12 glycans glycan NNS cord-329553-93tbgn2b 156 13 in in IN cord-329553-93tbgn2b 156 14 the the DT cord-329553-93tbgn2b 156 15 110ÁD 110ÁD NNP cord-329553-93tbgn2b 156 16 form form NN cord-329553-93tbgn2b 156 17 of of IN cord-329553-93tbgn2b 156 18 S33D s33d NN cord-329553-93tbgn2b 156 19 and and CC cord-329553-93tbgn2b 156 20 the the DT cord-329553-93tbgn2b 156 21 MAD mad JJ cord-329553-93tbgn2b 156 22 form form NN cord-329553-93tbgn2b 156 23 of of IN cord-329553-93tbgn2b 156 24 S64D s64d NN cord-329553-93tbgn2b 156 25 had have VBD cord-329553-93tbgn2b 156 26 been be VBN cord-329553-93tbgn2b 156 27 modified modify VBN cord-329553-93tbgn2b 156 28 by by IN cord-329553-93tbgn2b 156 29 enzymes enzyme NNS cord-329553-93tbgn2b 156 30 in in IN cord-329553-93tbgn2b 156 31 the the DT cord-329553-93tbgn2b 156 32 medial medial JJ cord-329553-93tbgn2b 156 33 - - HYPH cord-329553-93tbgn2b 156 34 Golgi Golgi NNP cord-329553-93tbgn2b 156 35 cisternae cisternae NN cord-329553-93tbgn2b 156 36 . . . cord-329553-93tbgn2b 157 1 These these DT cord-329553-93tbgn2b 157 2 results result NNS cord-329553-93tbgn2b 157 3 therefore therefore RB cord-329553-93tbgn2b 157 4 show show VBP cord-329553-93tbgn2b 157 5 that that IN cord-329553-93tbgn2b 157 6 both both CC cord-329553-93tbgn2b 157 7 fusion fusion NN cord-329553-93tbgn2b 157 8 proteins protein NNS cord-329553-93tbgn2b 157 9 were be VBD cord-329553-93tbgn2b 157 10 transported transport VBN cord-329553-93tbgn2b 157 11 efficiently efficiently RB cord-329553-93tbgn2b 157 12 from from IN cord-329553-93tbgn2b 157 13 the the DT cord-329553-93tbgn2b 157 14 ER ER NNP cord-329553-93tbgn2b 157 15 to to IN cord-329553-93tbgn2b 157 16 the the DT cord-329553-93tbgn2b 157 17 Golgi Golgi NNP cord-329553-93tbgn2b 157 18 apparatus apparatus NN cord-329553-93tbgn2b 157 19 and and CC cord-329553-93tbgn2b 157 20 that that IN cord-329553-93tbgn2b 157 21 they -PRON- PRP cord-329553-93tbgn2b 157 22 were be VBD cord-329553-93tbgn2b 157 23 localized localize VBN cord-329553-93tbgn2b 157 24 in in IN cord-329553-93tbgn2b 157 25 the the DT cord-329553-93tbgn2b 157 26 medial medial JJ cord-329553-93tbgn2b 157 27 - - HYPH cord-329553-93tbgn2b 157 28 and and CC cord-329553-93tbgn2b 157 29 / / SYM cord-329553-93tbgn2b 157 30 or or CC cord-329553-93tbgn2b 157 31 post post JJ cord-329553-93tbgn2b 157 32 - - JJ cord-329553-93tbgn2b 157 33 medial medial JJ cord-329553-93tbgn2b 157 34 - - HYPH cord-329553-93tbgn2b 157 35 Golgi golgi NN cord-329553-93tbgn2b 157 36 compartment compartment NN cord-329553-93tbgn2b 157 37 . . . cord-329553-93tbgn2b 158 1 Indirect indirect JJ cord-329553-93tbgn2b 158 2 immunofluorescence immunofluorescence NN cord-329553-93tbgn2b 158 3 microscopy microscopy NN cord-329553-93tbgn2b 158 4 was be VBD cord-329553-93tbgn2b 158 5 used use VBN cord-329553-93tbgn2b 158 6 to to TO cord-329553-93tbgn2b 158 7 determine determine VB cord-329553-93tbgn2b 158 8 the the DT cord-329553-93tbgn2b 158 9 cellular cellular JJ cord-329553-93tbgn2b 158 10 localization localization NN cord-329553-93tbgn2b 158 11 of of IN cord-329553-93tbgn2b 158 12 these these DT cord-329553-93tbgn2b 158 13 fusion fusion NN cord-329553-93tbgn2b 158 14 proteins protein NNS cord-329553-93tbgn2b 158 15 Cell cell NN cord-329553-93tbgn2b 158 16 lysates lysate NNS cord-329553-93tbgn2b 158 17 were be VBD cord-329553-93tbgn2b 158 18 immunoprecipitated immunoprecipitate VBN cord-329553-93tbgn2b 158 19 with with IN cord-329553-93tbgn2b 158 20 monoclonal monoclonal JJ cord-329553-93tbgn2b 158 21 antibodies antibody NNS cord-329553-93tbgn2b 158 22 to to IN cord-329553-93tbgn2b 158 23 DPPN DPPN NNP cord-329553-93tbgn2b 158 24 and and CC cord-329553-93tbgn2b 158 25 the the DT cord-329553-93tbgn2b 158 26 immunocomplexes immunocomplexe NNS cord-329553-93tbgn2b 158 27 were be VBD cord-329553-93tbgn2b 158 28 analyzed analyze VBN cord-329553-93tbgn2b 158 29 by by IN cord-329553-93tbgn2b 158 30 SDS SDS NNP cord-329553-93tbgn2b 158 31 - - HYPH cord-329553-93tbgn2b 158 32 PAGE PAGE NNP cord-329553-93tbgn2b 158 33 and and CC cord-329553-93tbgn2b 158 34 fluorography fluorography NN cord-329553-93tbgn2b 158 35 . . . cord-329553-93tbgn2b 159 1 The the DT cord-329553-93tbgn2b 159 2 smaller small JJR cord-329553-93tbgn2b 159 3 - - : cord-329553-93tbgn2b 159 4 and and CC cord-329553-93tbgn2b 159 5 the the DT cord-329553-93tbgn2b 159 6 larger large JJR cord-329553-93tbgn2b 159 7 - - HYPH cord-329553-93tbgn2b 159 8 sized sized JJ cord-329553-93tbgn2b 159 9 bands band NNS cord-329553-93tbgn2b 159 10 represent represent VBP cord-329553-93tbgn2b 159 11 the the DT cord-329553-93tbgn2b 159 12 ER ER NNP cord-329553-93tbgn2b 159 13 and and CC cord-329553-93tbgn2b 159 14 Golgi Golgi NNP cord-329553-93tbgn2b 159 15 forms form NNS cord-329553-93tbgn2b 159 16 , , , cord-329553-93tbgn2b 159 17 respectively respectively RB cord-329553-93tbgn2b 159 18 . . . cord-329553-93tbgn2b 160 1 ( ( -LRB- cord-329553-93tbgn2b 160 2 B b NN cord-329553-93tbgn2b 160 3 ) ) -RRB- cord-329553-93tbgn2b 161 1 Endo Endo NNP cord-329553-93tbgn2b 161 2 H H NNP cord-329553-93tbgn2b 161 3 treatment treatment NN cord-329553-93tbgn2b 161 4 of of IN cord-329553-93tbgn2b 161 5 fusion fusion NN cord-329553-93tbgn2b 161 6 proteins protein NNS cord-329553-93tbgn2b 162 1 S33D s33d JJ cord-329553-93tbgn2b 162 2 and and CC cord-329553-93tbgn2b 162 3 S64D. s64d. VB cord-329553-93tbgn2b 162 4 Cells cell NNS cord-329553-93tbgn2b 162 5 expressing express VBG cord-329553-93tbgn2b 162 6 the the DT cord-329553-93tbgn2b 162 7 respective respective JJ cord-329553-93tbgn2b 162 8 fusion fusion NN cord-329553-93tbgn2b 162 9 proteins protein NNS cord-329553-93tbgn2b 162 10 were be VBD cord-329553-93tbgn2b 162 11 labeled label VBN cord-329553-93tbgn2b 162 12 with with IN cord-329553-93tbgn2b 162 13 PIS]methionine pis]methionine PRP cord-329553-93tbgn2b 162 14 for for IN cord-329553-93tbgn2b 162 15 30 30 CD cord-329553-93tbgn2b 162 16 min min NN cord-329553-93tbgn2b 162 17 followed follow VBN cord-329553-93tbgn2b 162 18 by by IN cord-329553-93tbgn2b 162 19 30 30 CD cord-329553-93tbgn2b 162 20 min min NN cord-329553-93tbgn2b 162 21 of of IN cord-329553-93tbgn2b 162 22 chase chase NN cord-329553-93tbgn2b 162 23 . . . cord-329553-93tbgn2b 163 1 After after IN cord-329553-93tbgn2b 163 2 being be VBG cord-329553-93tbgn2b 163 3 immunoprecipitated immunoprecipitate VBN cord-329553-93tbgn2b 163 4 , , , cord-329553-93tbgn2b 163 5 half half NN cord-329553-93tbgn2b 163 6 of of IN cord-329553-93tbgn2b 163 7 the the DT cord-329553-93tbgn2b 163 8 sample sample NN cord-329553-93tbgn2b 163 9 was be VBD cord-329553-93tbgn2b 163 10 treated treat VBN cord-329553-93tbgn2b 163 11 with with IN cord-329553-93tbgn2b 163 12 endo endo NN cord-329553-93tbgn2b 163 13 H H NNP cord-329553-93tbgn2b 163 14 ( ( -LRB- cord-329553-93tbgn2b 163 15 + + CC cord-329553-93tbgn2b 163 16 ) ) -RRB- cord-329553-93tbgn2b 163 17 while while IN cord-329553-93tbgn2b 163 18 the the DT cord-329553-93tbgn2b 163 19 remaining remain VBG cord-329553-93tbgn2b 163 20 half half NN cord-329553-93tbgn2b 163 21 was be VBD cord-329553-93tbgn2b 163 22 processed process VBN cord-329553-93tbgn2b 163 23 in in IN cord-329553-93tbgn2b 163 24 an an DT cord-329553-93tbgn2b 163 25 identical identical JJ cord-329553-93tbgn2b 163 26 way way NN cord-329553-93tbgn2b 163 27 except except IN cord-329553-93tbgn2b 163 28 that that IN cord-329553-93tbgn2b 163 29 no no DT cord-329553-93tbgn2b 163 30 endo endo NN cord-329553-93tbgn2b 163 31 H h NN cord-329553-93tbgn2b 163 32 was be VBD cord-329553-93tbgn2b 163 33 included include VBN cord-329553-93tbgn2b 163 34 ( ( -LRB- cord-329553-93tbgn2b 163 35 - - : cord-329553-93tbgn2b 163 36 ) ) -RRB- cord-329553-93tbgn2b 163 37 . . . cord-329553-93tbgn2b 164 1 ( ( -LRB- cord-329553-93tbgn2b 164 2 Fig Fig NNP cord-329553-93tbgn2b 164 3 . . . cord-329553-93tbgn2b 164 4 4 4 CD cord-329553-93tbgn2b 164 5 A a NN cord-329553-93tbgn2b 164 6 ) ) -RRB- cord-329553-93tbgn2b 164 7 . . . cord-329553-93tbgn2b 165 1 An an DT cord-329553-93tbgn2b 165 2 intense intense JJ cord-329553-93tbgn2b 165 3 staining staining NN cord-329553-93tbgn2b 165 4 signal signal NN cord-329553-93tbgn2b 165 5 was be VBD cord-329553-93tbgn2b 165 6 detected detect VBN cord-329553-93tbgn2b 165 7 on on IN cord-329553-93tbgn2b 165 8 the the DT cord-329553-93tbgn2b 165 9 surface surface NN cord-329553-93tbgn2b 165 10 of of IN cord-329553-93tbgn2b 165 11 MDCK MDCK NNP cord-329553-93tbgn2b 165 12 cells cell NNS cord-329553-93tbgn2b 165 13 expressing express VBG cord-329553-93tbgn2b 165 14 normal normal JJ cord-329553-93tbgn2b 165 15 DPPN DPPN NNP cord-329553-93tbgn2b 165 16 ( ( -LRB- cord-329553-93tbgn2b 165 17 A a NN cord-329553-93tbgn2b 165 18 ) ) -RRB- cord-329553-93tbgn2b 165 19 . . . cord-329553-93tbgn2b 166 1 A a DT cord-329553-93tbgn2b 166 2 similar similar JJ cord-329553-93tbgn2b 166 3 staining staining NN cord-329553-93tbgn2b 166 4 pattern pattern NN cord-329553-93tbgn2b 166 5 was be VBD cord-329553-93tbgn2b 166 6 observed observe VBN cord-329553-93tbgn2b 166 7 for for IN cord-329553-93tbgn2b 166 8 normal normal JJ cord-329553-93tbgn2b 166 9 DPPIV DPPIV NNP cord-329553-93tbgn2b 166 10 in in IN cord-329553-93tbgn2b 166 11 permeabilized permeabilized JJ cord-329553-93tbgn2b 166 12 cells cell NNS cord-329553-93tbgn2b 166 13 ( ( -LRB- cord-329553-93tbgn2b 166 14 B b NN cord-329553-93tbgn2b 166 15 ) ) -RRB- cord-329553-93tbgn2b 166 16 except except IN cord-329553-93tbgn2b 166 17 that that IN cord-329553-93tbgn2b 166 18 some some DT cord-329553-93tbgn2b 166 19 perinuclear perinuclear JJ cord-329553-93tbgn2b 166 20 signal signal NN cord-329553-93tbgn2b 166 21 was be VBD cord-329553-93tbgn2b 166 22 also also RB cord-329553-93tbgn2b 166 23 detected detect VBN cord-329553-93tbgn2b 166 24 . . . cord-329553-93tbgn2b 167 1 The the DT cord-329553-93tbgn2b 167 2 perinuclear perinuclear NN cord-329553-93tbgn2b 167 3 staining stain VBG cord-329553-93tbgn2b 167 4 most most RBS cord-329553-93tbgn2b 167 5 likely likely RB cord-329553-93tbgn2b 167 6 represents represent VBZ cord-329553-93tbgn2b 167 7 molecules molecule NNS cord-329553-93tbgn2b 167 8 in in IN cord-329553-93tbgn2b 167 9 transit transit NN cord-329553-93tbgn2b 167 10 through through IN cord-329553-93tbgn2b 167 11 the the DT cord-329553-93tbgn2b 167 12 Golgi Golgi NNP cord-329553-93tbgn2b 167 13 apparatus apparatus NN cord-329553-93tbgn2b 167 14 . . . cord-329553-93tbgn2b 168 1 In in IN cord-329553-93tbgn2b 168 2 contrast contrast NN cord-329553-93tbgn2b 168 3 , , , cord-329553-93tbgn2b 168 4 only only RB cord-329553-93tbgn2b 168 5 background background NN cord-329553-93tbgn2b 168 6 staining staining NN cord-329553-93tbgn2b 168 7 was be VBD cord-329553-93tbgn2b 168 8 observed observe VBN cord-329553-93tbgn2b 168 9 on on IN cord-329553-93tbgn2b 168 10 the the DT cord-329553-93tbgn2b 168 11 surface surface NN cord-329553-93tbgn2b 168 12 of of IN cord-329553-93tbgn2b 168 13 cells cell NNS cord-329553-93tbgn2b 168 14 expressing express VBG cord-329553-93tbgn2b 168 15 S33D s33d RB cord-329553-93tbgn2b 168 16 ( ( -LRB- cord-329553-93tbgn2b 168 17 C C NNP cord-329553-93tbgn2b 168 18 ) ) -RRB- cord-329553-93tbgn2b 168 19 and and CC cord-329553-93tbgn2b 168 20 S64D S64D NNP cord-329553-93tbgn2b 168 21 ( ( -LRB- cord-329553-93tbgn2b 168 22 E E NNP cord-329553-93tbgn2b 168 23 ) ) -RRB- cord-329553-93tbgn2b 168 24 . . . cord-329553-93tbgn2b 169 1 When when WRB cord-329553-93tbgn2b 169 2 the the DT cord-329553-93tbgn2b 169 3 cells cell NNS cord-329553-93tbgn2b 169 4 were be VBD cord-329553-93tbgn2b 169 5 permeabilized permeabilize VBN cord-329553-93tbgn2b 169 6 , , , cord-329553-93tbgn2b 169 7 intense intense JJ cord-329553-93tbgn2b 169 8 perinuclear perinuclear NNP cord-329553-93tbgn2b 169 9 vesiculartubular vesiculartubular NNP cord-329553-93tbgn2b 169 10 staining staining NN cord-329553-93tbgn2b 169 11 was be VBD cord-329553-93tbgn2b 169 12 detected detect VBN cord-329553-93tbgn2b 169 13 in in IN cord-329553-93tbgn2b 169 14 the the DT cord-329553-93tbgn2b 169 15 cells cell NNS cord-329553-93tbgn2b 169 16 expressing express VBG cord-329553-93tbgn2b 169 17 S33D s33d JJ cord-329553-93tbgn2b 169 18 ( ( -LRB- cord-329553-93tbgn2b 169 19 D D NNP cord-329553-93tbgn2b 169 20 ) ) -RRB- cord-329553-93tbgn2b 169 21 and and CC cord-329553-93tbgn2b 169 22 S64D S64D NNP cord-329553-93tbgn2b 169 23 ( ( -LRB- cord-329553-93tbgn2b 169 24 F F NNP cord-329553-93tbgn2b 169 25 ) ) -RRB- cord-329553-93tbgn2b 169 26 . . . cord-329553-93tbgn2b 170 1 This this DT cord-329553-93tbgn2b 170 2 pattern pattern NN cord-329553-93tbgn2b 170 3 of of IN cord-329553-93tbgn2b 170 4 intracellular intracellular JJ cord-329553-93tbgn2b 170 5 staining staining NN cord-329553-93tbgn2b 170 6 is be VBZ cord-329553-93tbgn2b 170 7 typical typical JJ cord-329553-93tbgn2b 170 8 for for IN cord-329553-93tbgn2b 170 9 the the DT cord-329553-93tbgn2b 170 10 Golgi Golgi NNP cord-329553-93tbgn2b 170 11 apparatus apparatus NN cord-329553-93tbgn2b 170 12 ( ( -LRB- cord-329553-93tbgn2b 170 13 Lipsky Lipsky NNP cord-329553-93tbgn2b 170 14 and and CC cord-329553-93tbgn2b 170 15 Pagano Pagano NNP cord-329553-93tbgn2b 170 16 , , , cord-329553-93tbgn2b 170 17 1985 1985 CD cord-329553-93tbgn2b 170 18 ; ; : cord-329553-93tbgn2b 170 19 Louvard Louvard NNP cord-329553-93tbgn2b 170 20 et et NNP cord-329553-93tbgn2b 170 21 al al NNP cord-329553-93tbgn2b 170 22 . . NNP cord-329553-93tbgn2b 170 23 , , , cord-329553-93tbgn2b 170 24 1982 1982 CD cord-329553-93tbgn2b 170 25 ; ; : cord-329553-93tbgn2b 170 26 Saraste Saraste NNP cord-329553-93tbgn2b 170 27 et et NNP cord-329553-93tbgn2b 170 28 al al NNP cord-329553-93tbgn2b 170 29 . . NNP cord-329553-93tbgn2b 170 30 , , , cord-329553-93tbgn2b 170 31 1987 1987 CD cord-329553-93tbgn2b 170 32 ) ) -RRB- cord-329553-93tbgn2b 170 33 , , , cord-329553-93tbgn2b 170 34 suggesting suggest VBG cord-329553-93tbgn2b 170 35 that that IN cord-329553-93tbgn2b 170 36 S33D s33d NN cord-329553-93tbgn2b 170 37 and and CC cord-329553-93tbgn2b 170 38 S64D s64d NN cord-329553-93tbgn2b 170 39 are be VBP cord-329553-93tbgn2b 170 40 predominantly predominantly RB cord-329553-93tbgn2b 170 41 localized localize VBN cord-329553-93tbgn2b 170 42 to to IN cord-329553-93tbgn2b 170 43 the the DT cord-329553-93tbgn2b 170 44 Golgi Golgi NNP cord-329553-93tbgn2b 170 45 apparatus apparatus NN cord-329553-93tbgn2b 170 46 . . . cord-329553-93tbgn2b 171 1 To to TO cord-329553-93tbgn2b 171 2 further further RB cord-329553-93tbgn2b 171 3 confirm confirm VB cord-329553-93tbgn2b 171 4 the the DT cord-329553-93tbgn2b 171 5 Golgi Golgi NNP cord-329553-93tbgn2b 171 6 localization localization NN cord-329553-93tbgn2b 171 7 of of IN cord-329553-93tbgn2b 171 8 S33D s33d NN cord-329553-93tbgn2b 171 9 and and CC cord-329553-93tbgn2b 171 10 S64D s64d NN cord-329553-93tbgn2b 171 11 , , , cord-329553-93tbgn2b 171 12 colocalization colocalization NN cord-329553-93tbgn2b 171 13 of of IN cord-329553-93tbgn2b 171 14 S33D s33d JJ cord-329553-93tbgn2b 171 15 or or CC cord-329553-93tbgn2b 171 16 S64D s64d NN cord-329553-93tbgn2b 171 17 with with IN cord-329553-93tbgn2b 171 18 intracellular intracellular JJ cord-329553-93tbgn2b 171 19 WGA WGA NNP cord-329553-93tbgn2b 171 20 binding bind VBG cord-329553-93tbgn2b 171 21 sites site NNS cord-329553-93tbgn2b 171 22 was be VBD cord-329553-93tbgn2b 171 23 carried carry VBN cord-329553-93tbgn2b 171 24 out out RP cord-329553-93tbgn2b 171 25 . . . cord-329553-93tbgn2b 172 1 Intracellular Intracellular NNP cord-329553-93tbgn2b 172 2 WGA WGA NNP cord-329553-93tbgn2b 172 3 binding bind VBG cord-329553-93tbgn2b 172 4 sites site NNS cord-329553-93tbgn2b 172 5 are be VBP cord-329553-93tbgn2b 172 6 present present JJ cord-329553-93tbgn2b 172 7 predominantly predominantly RB cord-329553-93tbgn2b 172 8 in in IN cord-329553-93tbgn2b 172 9 the the DT cord-329553-93tbgn2b 172 10 Golgi Golgi NNP cord-329553-93tbgn2b 172 11 apparatus apparatus NN cord-329553-93tbgn2b 172 12 ( ( -LRB- cord-329553-93tbgn2b 172 13 Lipsky Lipsky NNP cord-329553-93tbgn2b 172 14 and and CC cord-329553-93tbgn2b 172 15 Pagano Pagano NNP cord-329553-93tbgn2b 172 16 , , , cord-329553-93tbgn2b 172 17 1985 1985 CD cord-329553-93tbgn2b 172 18 ; ; : cord-329553-93tbgn2b 172 19 Tartakoff Tartakoff NNP cord-329553-93tbgn2b 172 20 and and CC cord-329553-93tbgn2b 172 21 Vassalli Vassalli NNP cord-329553-93tbgn2b 172 22 , , , cord-329553-93tbgn2b 172 23 1983 1983 CD cord-329553-93tbgn2b 172 24 ) ) -RRB- cord-329553-93tbgn2b 172 25 . . . cord-329553-93tbgn2b 173 1 As as IN cord-329553-93tbgn2b 173 2 shown show VBN cord-329553-93tbgn2b 173 3 in in IN cord-329553-93tbgn2b 173 4 Fig Fig NNP cord-329553-93tbgn2b 173 5 . . . cord-329553-93tbgn2b 174 1 4 4 CD cord-329553-93tbgn2b 174 2 B b NN cord-329553-93tbgn2b 174 3 , , , cord-329553-93tbgn2b 174 4 the the DT cord-329553-93tbgn2b 174 5 staining staining NN cord-329553-93tbgn2b 174 6 for for IN cord-329553-93tbgn2b 174 7 S33D s33d JJ cord-329553-93tbgn2b 174 8 ( ( -LRB- cord-329553-93tbgn2b 174 9 A a NN cord-329553-93tbgn2b 174 10 ) ) -RRB- cord-329553-93tbgn2b 174 11 and and CC cord-329553-93tbgn2b 174 12 S64D S64D NNP cord-329553-93tbgn2b 174 13 ( ( -LRB- cord-329553-93tbgn2b 174 14 C C NNP cord-329553-93tbgn2b 174 15 ) ) -RRB- cord-329553-93tbgn2b 174 16 colocalized colocalize VBN cord-329553-93tbgn2b 174 17 well well RB cord-329553-93tbgn2b 174 18 with with IN cord-329553-93tbgn2b 174 19 that that DT cord-329553-93tbgn2b 174 20 for for IN cord-329553-93tbgn2b 174 21 WGA WGA NNP cord-329553-93tbgn2b 174 22 binding bind VBG cord-329553-93tbgn2b 174 23 sites site NNS cord-329553-93tbgn2b 174 24 ( ( -LRB- cord-329553-93tbgn2b 174 25 B b NN cord-329553-93tbgn2b 174 26 and and CC cord-329553-93tbgn2b 174 27 D D NNP cord-329553-93tbgn2b 174 28 ) ) -RRB- cord-329553-93tbgn2b 174 29 . . . cord-329553-93tbgn2b 175 1 These these DT cord-329553-93tbgn2b 175 2 results result NNS cord-329553-93tbgn2b 175 3 demonstrated demonstrate VBD cord-329553-93tbgn2b 175 4 that that IN cord-329553-93tbgn2b 175 5 S33D s33d NN cord-329553-93tbgn2b 175 6 and and CC cord-329553-93tbgn2b 175 7 S64D s64d NN cord-329553-93tbgn2b 175 8 were be VBD cord-329553-93tbgn2b 175 9 indeed indeed RB cord-329553-93tbgn2b 175 10 localized localized JJ cord-329553-93tbgn2b 175 11 to to IN cord-329553-93tbgn2b 175 12 the the DT cord-329553-93tbgn2b 175 13 Golgi Golgi NNP cord-329553-93tbgn2b 175 14 apparatus apparatus NN cord-329553-93tbgn2b 175 15 . . . cord-329553-93tbgn2b 176 1 Localization localization NN cord-329553-93tbgn2b 176 2 ofS33D ofS33D NNP cord-329553-93tbgn2b 176 3 and and CC cord-329553-93tbgn2b 176 4 S64D s64d NN cord-329553-93tbgn2b 176 5 to to IN cord-329553-93tbgn2b 176 6 the the DT cord-329553-93tbgn2b 176 7 Trans Trans NNP cord-329553-93tbgn2b 176 8 - - NNP cord-329553-93tbgn2b 176 9 Golgi Golgi NNP cord-329553-93tbgn2b 176 10 Cisternae Cisternae NNP cord-329553-93tbgn2b 177 1 and/or and/or CC cord-329553-93tbgn2b 177 2 TGN TGN NNP cord-329553-93tbgn2b 178 1 The the DT cord-329553-93tbgn2b 178 2 results result NNS cord-329553-93tbgn2b 178 3 obtained obtain VBN cord-329553-93tbgn2b 178 4 from from IN cord-329553-93tbgn2b 178 5 pulse pulse NN cord-329553-93tbgn2b 178 6 - - HYPH cord-329553-93tbgn2b 178 7 chase chase NN cord-329553-93tbgn2b 178 8 and and CC cord-329553-93tbgn2b 178 9 endo endo NN cord-329553-93tbgn2b 178 10 H H NNP cord-329553-93tbgn2b 178 11 treatment treatment NN cord-329553-93tbgn2b 178 12 experiments experiment NNS cord-329553-93tbgn2b 178 13 suggest suggest VBP cord-329553-93tbgn2b 178 14 that that IN cord-329553-93tbgn2b 178 15 S33D S33D : cord-329553-93tbgn2b 178 16 and and CC cord-329553-93tbgn2b 178 17 S64D s64d NN cord-329553-93tbgn2b 178 18 were be VBD cord-329553-93tbgn2b 178 19 present present JJ cord-329553-93tbgn2b 178 20 in in IN cord-329553-93tbgn2b 178 21 the the DT cord-329553-93tbgn2b 178 22 medial medial JJ cord-329553-93tbgn2b 178 23 - - HYPH cord-329553-93tbgn2b 178 24 and and CC cord-329553-93tbgn2b 178 25 / / SYM cord-329553-93tbgn2b 178 26 or or CC cord-329553-93tbgn2b 178 27 post post JJ cord-329553-93tbgn2b 178 28 - - JJ cord-329553-93tbgn2b 178 29 medial medial JJ cord-329553-93tbgn2b 178 30 - - HYPH cord-329553-93tbgn2b 178 31 Golgi Golgi NNP cord-329553-93tbgn2b 178 32 cisternae cisternae NN cord-329553-93tbgn2b 178 33 . . . cord-329553-93tbgn2b 179 1 The the DT cord-329553-93tbgn2b 179 2 indirect indirect JJ cord-329553-93tbgn2b 179 3 fluorescence fluorescence NN cord-329553-93tbgn2b 179 4 microscopy microscopy NNS cord-329553-93tbgn2b 179 5 demonstrated demonstrate VBD cord-329553-93tbgn2b 179 6 that that IN cord-329553-93tbgn2b 179 7 they -PRON- PRP cord-329553-93tbgn2b 179 8 were be VBD cord-329553-93tbgn2b 179 9 localized localize VBN cord-329553-93tbgn2b 179 10 in in IN cord-329553-93tbgn2b 179 11 the the DT cord-329553-93tbgn2b 179 12 Golgi Golgi NNP cord-329553-93tbgn2b 179 13 apparatus apparatus NN cord-329553-93tbgn2b 179 14 . . . cord-329553-93tbgn2b 180 1 To to TO cord-329553-93tbgn2b 180 2 further further RB cord-329553-93tbgn2b 180 3 determine determine VB cord-329553-93tbgn2b 180 4 the the DT cord-329553-93tbgn2b 180 5 compartment compartment NN cord-329553-93tbgn2b 180 6 of of IN cord-329553-93tbgn2b 180 7 their -PRON- PRP$ cord-329553-93tbgn2b 180 8 localization localization NN cord-329553-93tbgn2b 180 9 , , , cord-329553-93tbgn2b 180 10 their -PRON- PRP$ cord-329553-93tbgn2b 180 11 N n CD cord-329553-93tbgn2b 180 12 - - HYPH cord-329553-93tbgn2b 180 13 linked link VBN cord-329553-93tbgn2b 180 14 carbohydrate carbohydrate NN cord-329553-93tbgn2b 180 15 structures structure NNS cord-329553-93tbgn2b 180 16 were be VBD cord-329553-93tbgn2b 180 17 examined examine VBN cord-329553-93tbgn2b 180 18 by by IN cord-329553-93tbgn2b 180 19 lectin lectin NN cord-329553-93tbgn2b 180 20 affinity affinity NN cord-329553-93tbgn2b 180 21 chromatography chromatography NN cord-329553-93tbgn2b 180 22 ( ( -LRB- cord-329553-93tbgn2b 180 23 Haselbeck Haselbeck NNP cord-329553-93tbgn2b 180 24 et et NNP cord-329553-93tbgn2b 180 25 al al NNP cord-329553-93tbgn2b 180 26 . . NNP cord-329553-93tbgn2b 180 27 , , , cord-329553-93tbgn2b 180 28 1990 1990 CD cord-329553-93tbgn2b 180 29 ; ; : cord-329553-93tbgn2b 180 30 Lis Lis NNP cord-329553-93tbgn2b 180 31 and and CC cord-329553-93tbgn2b 180 32 Sharon Sharon NNP cord-329553-93tbgn2b 180 33 , , , cord-329553-93tbgn2b 180 34 1986 1986 CD cord-329553-93tbgn2b 180 35 ) ) -RRB- cord-329553-93tbgn2b 181 1 ( ( -LRB- cord-329553-93tbgn2b 181 2 Fig Fig NNP cord-329553-93tbgn2b 181 3 . . NNP cord-329553-93tbgn2b 181 4 5 5 CD cord-329553-93tbgn2b 181 5 ) ) -RRB- cord-329553-93tbgn2b 181 6 . . . cord-329553-93tbgn2b 182 1 Cells cell NNS cord-329553-93tbgn2b 182 2 expressing express VBG cord-329553-93tbgn2b 182 3 S33D s33d JJ cord-329553-93tbgn2b 182 4 were be VBD cord-329553-93tbgn2b 182 5 pulse pulse NN cord-329553-93tbgn2b 182 6 labeled label VBN cord-329553-93tbgn2b 182 7 with with IN cord-329553-93tbgn2b 182 8 [ [ -LRB- cord-329553-93tbgn2b 182 9 35S]methionine 35s]methionine CD cord-329553-93tbgn2b 182 10 followed follow VBN cord-329553-93tbgn2b 182 11 by by IN cord-329553-93tbgn2b 182 12 30 30 CD cord-329553-93tbgn2b 182 13 min min NN cord-329553-93tbgn2b 182 14 of of IN cord-329553-93tbgn2b 182 15 chase chase NNP cord-329553-93tbgn2b 183 1 such such JJ cord-329553-93tbgn2b 183 2 that that IN cord-329553-93tbgn2b 183 3 both both CC cord-329553-93tbgn2b 183 4 the the DT cord-329553-93tbgn2b 183 5 100-kD 100-kd CD cord-329553-93tbgn2b 183 6 ER ER NNP cord-329553-93tbgn2b 183 7 form form NN cord-329553-93tbgn2b 183 8 and and CC cord-329553-93tbgn2b 183 9 the the DT cord-329553-93tbgn2b 183 10 110-kD 110-kd CD cord-329553-93tbgn2b 183 11 Golgi Golgi NNP cord-329553-93tbgn2b 183 12 form form NN cord-329553-93tbgn2b 183 13 of of IN cord-329553-93tbgn2b 183 14 S33D s33d JJ cord-329553-93tbgn2b 183 15 were be VBD cord-329553-93tbgn2b 183 16 present present JJ cord-329553-93tbgn2b 183 17 in in IN cord-329553-93tbgn2b 183 18 the the DT cord-329553-93tbgn2b 183 19 cells cell NNS cord-329553-93tbgn2b 183 20 . . . cord-329553-93tbgn2b 184 1 Similar similar JJ cord-329553-93tbgn2b 184 2 amounts amount NNS cord-329553-93tbgn2b 184 3 of of IN cord-329553-93tbgn2b 184 4 labeled label VBN cord-329553-93tbgn2b 184 5 S33D s33d JJ cord-329553-93tbgn2b 184 6 were be VBD cord-329553-93tbgn2b 184 7 incubated incubate VBN cord-329553-93tbgn2b 184 8 with with IN cord-329553-93tbgn2b 184 9 various various JJ cord-329553-93tbgn2b 184 10 lectin lectin NN cord-329553-93tbgn2b 184 11 - - HYPH cord-329553-93tbgn2b 184 12 agarose agarose VBN cord-329553-93tbgn2b 184 13 beads bead NNS cord-329553-93tbgn2b 184 14 . . . cord-329553-93tbgn2b 185 1 The the DT cord-329553-93tbgn2b 185 2 material material NN cord-329553-93tbgn2b 185 3 bound bind VBN cord-329553-93tbgn2b 185 4 to to IN cord-329553-93tbgn2b 185 5 the the DT cord-329553-93tbgn2b 185 6 beads bead NNS cord-329553-93tbgn2b 185 7 was be VBD cord-329553-93tbgn2b 185 8 analyzed analyze VBN cord-329553-93tbgn2b 185 9 . . . cord-329553-93tbgn2b 186 1 As as IN cord-329553-93tbgn2b 186 2 shown show VBN cord-329553-93tbgn2b 186 3 in in IN cord-329553-93tbgn2b 186 4 Fig Fig NNP cord-329553-93tbgn2b 186 5 . . . cord-329553-93tbgn2b 187 1 5 5 LS cord-329553-93tbgn2b 187 2 A a NN cord-329553-93tbgn2b 187 3 , , , cord-329553-93tbgn2b 187 4 both both CC cord-329553-93tbgn2b 187 5 the the DT cord-329553-93tbgn2b 187 6 100-and 100-and CD cord-329553-93tbgn2b 187 7 11041 11041 CD cord-329553-93tbgn2b 187 8 ) ) -RRB- cord-329553-93tbgn2b 188 1 forms form NNS cord-329553-93tbgn2b 188 2 could could MD cord-329553-93tbgn2b 188 3 be be VB cord-329553-93tbgn2b 188 4 quantitatively quantitatively RB cord-329553-93tbgn2b 188 5 recovered recover VBN cord-329553-93tbgn2b 188 6 with with IN cord-329553-93tbgn2b 188 7 Con Con NNP cord-329553-93tbgn2b 188 8 A A NNP cord-329553-93tbgn2b 188 9 - - HYPH cord-329553-93tbgn2b 188 10 agarose agarose JJ cord-329553-93tbgn2b 188 11 ( ( -LRB- cord-329553-93tbgn2b 188 12 mannose mannose NN cord-329553-93tbgn2b 188 13 - - HYPH cord-329553-93tbgn2b 188 14 binding bind VBG cord-329553-93tbgn2b 188 15 lectin lectin NN cord-329553-93tbgn2b 188 16 ) ) -RRB- cord-329553-93tbgn2b 189 1 ( ( -LRB- cord-329553-93tbgn2b 189 2 lane lane NNP cord-329553-93tbgn2b 189 3 2 2 CD cord-329553-93tbgn2b 189 4 ) ) -RRB- cord-329553-93tbgn2b 189 5 , , , cord-329553-93tbgn2b 189 6 consistent consistent JJ cord-329553-93tbgn2b 189 7 with with IN cord-329553-93tbgn2b 189 8 the the DT cord-329553-93tbgn2b 189 9 presence presence NN cord-329553-93tbgn2b 189 10 of of IN cord-329553-93tbgn2b 189 11 N n NN cord-329553-93tbgn2b 189 12 - - HYPH cord-329553-93tbgn2b 189 13 linked link VBN cord-329553-93tbgn2b 189 14 glycans glycan NNS cord-329553-93tbgn2b 189 15 of of IN cord-329553-93tbgn2b 189 16 high high JJ cord-329553-93tbgn2b 189 17 - - HYPH cord-329553-93tbgn2b 189 18 mannose mannose NN cord-329553-93tbgn2b 189 19 type type NN cord-329553-93tbgn2b 189 20 in in IN cord-329553-93tbgn2b 189 21 both both DT cord-329553-93tbgn2b 189 22 100-and 100-and CD cord-329553-93tbgn2b 189 23 110-kD 110-kd CD cord-329553-93tbgn2b 189 24 forms form NNS cord-329553-93tbgn2b 189 25 of of IN cord-329553-93tbgn2b 189 26 S33D s33d RB cord-329553-93tbgn2b 189 27 as as IN cord-329553-93tbgn2b 189 28 concluded conclude VBN cord-329553-93tbgn2b 189 29 from from IN cord-329553-93tbgn2b 189 30 the the DT cord-329553-93tbgn2b 189 31 endo endo NN cord-329553-93tbgn2b 189 32 H H NNP cord-329553-93tbgn2b 189 33 experiment experiment NN cord-329553-93tbgn2b 189 34 . . . cord-329553-93tbgn2b 190 1 The the DT cord-329553-93tbgn2b 190 2 110-but 110-but CD cord-329553-93tbgn2b 190 3 not not RB cord-329553-93tbgn2b 190 4 the the DT cord-329553-93tbgn2b 190 5 100-kD 100-kd CD cord-329553-93tbgn2b 190 6 form form NN cord-329553-93tbgn2b 190 7 was be VBD cord-329553-93tbgn2b 190 8 also also RB cord-329553-93tbgn2b 190 9 recovered recover VBN cord-329553-93tbgn2b 190 10 from from IN cord-329553-93tbgn2b 190 11 WGA- wga- JJ cord-329553-93tbgn2b 190 12 , , , cord-329553-93tbgn2b 190 13 Erythrina Erythrina NNP cord-329553-93tbgn2b 190 14 cristagalli cristagalli NN cord-329553-93tbgn2b 190 15 , , , cord-329553-93tbgn2b 190 16 ( ( -LRB- cord-329553-93tbgn2b 190 17 ECA)- ECA)- NNP cord-329553-93tbgn2b 190 18 , , , cord-329553-93tbgn2b 190 19 Sambucus Sambucus NNP cord-329553-93tbgn2b 190 20 nigra nigra NN cord-329553-93tbgn2b 190 21 ( ( -LRB- cord-329553-93tbgn2b 190 22 SNA)- SNA)- NNP cord-329553-93tbgn2b 190 23 , , , cord-329553-93tbgn2b 190 24 and and CC cord-329553-93tbgn2b 190 25 Maackia Maackia NNP cord-329553-93tbgn2b 190 26 amurensis amurensis NN cord-329553-93tbgn2b 190 27 ( ( -LRB- cord-329553-93tbgn2b 190 28 MAA)-agarose maa)-agarose JJ cord-329553-93tbgn2b 190 29 beads bead NNS cord-329553-93tbgn2b 190 30 . . . cord-329553-93tbgn2b 191 1 Since since IN cord-329553-93tbgn2b 191 2 ECA ECA NNP cord-329553-93tbgn2b 191 3 binds bind VBZ cord-329553-93tbgn2b 191 4 specifically specifically RB cord-329553-93tbgn2b 191 5 to to IN cord-329553-93tbgn2b 191 6 the the DT cord-329553-93tbgn2b 191 7 Gal-01,4-G1cNac Gal-01,4-G1cNac NNP cord-329553-93tbgn2b 191 8 structure structure NN cord-329553-93tbgn2b 191 9 in in IN cord-329553-93tbgn2b 191 10 N n CD cord-329553-93tbgn2b 191 11 - - HYPH cord-329553-93tbgn2b 191 12 linked link VBN cord-329553-93tbgn2b 191 13 glycans glycan NNS cord-329553-93tbgn2b 191 14 , , , cord-329553-93tbgn2b 191 15 this this DT cord-329553-93tbgn2b 191 16 result result NN cord-329553-93tbgn2b 191 17 demonstrates demonstrate VBZ cord-329553-93tbgn2b 191 18 that that IN cord-329553-93tbgn2b 191 19 the the DT cord-329553-93tbgn2b 191 20 110-kD 110-kd CD cord-329553-93tbgn2b 191 21 form form NN cord-329553-93tbgn2b 191 22 has have VBZ cord-329553-93tbgn2b 191 23 been be VBN cord-329553-93tbgn2b 191 24 modified modify VBN cord-329553-93tbgn2b 191 25 by by IN cord-329553-93tbgn2b 191 26 trans trans NNP cord-329553-93tbgn2b 191 27 - - NNP cord-329553-93tbgn2b 191 28 Golgi Golgi NNP cord-329553-93tbgn2b 191 29 GT GT NNP cord-329553-93tbgn2b 191 30 . . . cord-329553-93tbgn2b 192 1 SNA SNA NNP cord-329553-93tbgn2b 192 2 and and CC cord-329553-93tbgn2b 192 3 MAA MAA NNP cord-329553-93tbgn2b 192 4 bind bind VBP cord-329553-93tbgn2b 192 5 a-2,6-and a-2,6-and NNP cord-329553-93tbgn2b 192 6 a-2,3linked a-2,3linked JJ cord-329553-93tbgn2b 192 7 sialic sialic JJ cord-329553-93tbgn2b 192 8 acids acid NNS cord-329553-93tbgn2b 192 9 , , , cord-329553-93tbgn2b 192 10 respectively respectively RB cord-329553-93tbgn2b 192 11 . . . cord-329553-93tbgn2b 193 1 Thus thus RB cord-329553-93tbgn2b 193 2 the the DT cord-329553-93tbgn2b 193 3 110-kD 110-kd CD cord-329553-93tbgn2b 193 4 form form NN cord-329553-93tbgn2b 193 5 of of IN cord-329553-93tbgn2b 193 6 S33D s33d NN cord-329553-93tbgn2b 193 7 has have VBZ cord-329553-93tbgn2b 193 8 been be VBN cord-329553-93tbgn2b 193 9 modified modify VBN cord-329553-93tbgn2b 193 10 byboth byboth JJ cord-329553-93tbgn2b 193 11 a-2,6-and a-2,6-and . cord-329553-93tbgn2b 193 12 a-2,3-sialyltransferases a-2,3-sialyltransferases NNPS cord-329553-93tbgn2b 193 13 , , , cord-329553-93tbgn2b 193 14 both both DT cord-329553-93tbgn2b 193 15 of of IN cord-329553-93tbgn2b 193 16 which which WDT cord-329553-93tbgn2b 193 17 are be VBP cord-329553-93tbgn2b 193 18 localized localize VBN cord-329553-93tbgn2b 193 19 to to IN cord-329553-93tbgn2b 193 20 the the DT cord-329553-93tbgn2b 193 21 trans trans NNP cord-329553-93tbgn2b 193 22 - - NNP cord-329553-93tbgn2b 193 23 Golgi Golgi NNP cord-329553-93tbgn2b 193 24 cisternae cisternae NNP cord-329553-93tbgn2b 193 25 and/or and/or CC cord-329553-93tbgn2b 193 26 TGN TGN NNP cord-329553-93tbgn2b 193 27 . . . cord-329553-93tbgn2b 194 1 These these DT cord-329553-93tbgn2b 194 2 results result NNS cord-329553-93tbgn2b 194 3 demonstrate demonstrate VBP cord-329553-93tbgn2b 194 4 that that IN cord-329553-93tbgn2b 194 5 S33D s33d RB cord-329553-93tbgn2b 194 6 had have VBD cord-329553-93tbgn2b 194 7 been be VBN cord-329553-93tbgn2b 194 8 modified modify VBN cord-329553-93tbgn2b 194 9 by by IN cord-329553-93tbgn2b 194 10 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 194 11 of of IN cord-329553-93tbgn2b 194 12 the the DT cord-329553-93tbgn2b 194 13 trans trans NNP cord-329553-93tbgn2b 194 14 - - NNP cord-329553-93tbgn2b 194 15 Golgi Golgi NNP cord-329553-93tbgn2b 194 16 cisternae cisternae NNP cord-329553-93tbgn2b 194 17 and and CC cord-329553-93tbgn2b 194 18 the the DT cord-329553-93tbgn2b 194 19 TGN TGN NNP cord-329553-93tbgn2b 194 20 and and CC cord-329553-93tbgn2b 194 21 suggest suggest VBP cord-329553-93tbgn2b 194 22 that that IN cord-329553-93tbgn2b 194 23 it -PRON- PRP cord-329553-93tbgn2b 194 24 is be VBZ cord-329553-93tbgn2b 194 25 localized localize VBN cord-329553-93tbgn2b 194 26 to to IN cord-329553-93tbgn2b 194 27 the the DT cord-329553-93tbgn2b 194 28 trans trans NNP cord-329553-93tbgn2b 194 29 - - NNP cord-329553-93tbgn2b 194 30 Golgi Golgi NNP cord-329553-93tbgn2b 194 31 cisternae cisternae NN cord-329553-93tbgn2b 195 1 and/or and/or CC cord-329553-93tbgn2b 195 2 TGN TGN NNP cord-329553-93tbgn2b 195 3 . . . cord-329553-93tbgn2b 196 1 WGA WGA NNP cord-329553-93tbgn2b 196 2 binds bind VBZ cord-329553-93tbgn2b 196 3 both both DT cord-329553-93tbgn2b 196 4 sialic sialic JJ cord-329553-93tbgn2b 196 5 acid acid NN cord-329553-93tbgn2b 196 6 and and CC cord-329553-93tbgn2b 196 7 N n CD cord-329553-93tbgn2b 196 8 - - HYPH cord-329553-93tbgn2b 196 9 acetylglucosamine acetylglucosamine NNP cord-329553-93tbgn2b 196 10 while while IN cord-329553-93tbgn2b 196 11 succinylated succinylated JJ cord-329553-93tbgn2b 196 12 WGA WGA NNP cord-329553-93tbgn2b 196 13 ( ( -LRB- cord-329553-93tbgn2b 196 14 sWGA sWGA NNP cord-329553-93tbgn2b 196 15 ) ) -RRB- cord-329553-93tbgn2b 196 16 binds bind VBZ cord-329553-93tbgn2b 196 17 only only RB cord-329553-93tbgn2b 196 18 N n CD cord-329553-93tbgn2b 196 19 - - HYPH cord-329553-93tbgn2b 196 20 acetylglucosamine acetylglucosamine NNP cord-329553-93tbgn2b 197 1 ( ( -LRB- cord-329553-93tbgn2b 197 2 Monsigny Monsigny NNP cord-329553-93tbgn2b 197 3 et et NNP cord-329553-93tbgn2b 197 4 al al NNP cord-329553-93tbgn2b 197 5 . . . cord-329553-93tbgn2b 198 1 , , , cord-329553-93tbgn2b 198 2 1979 1979 CD cord-329553-93tbgn2b 198 3 ( ( -LRB- cord-329553-93tbgn2b 198 4 Monsigny Monsigny NNP cord-329553-93tbgn2b 198 5 et et NNP cord-329553-93tbgn2b 198 6 al al NNP cord-329553-93tbgn2b 198 7 . . NNP cord-329553-93tbgn2b 198 8 , , , cord-329553-93tbgn2b 198 9 , , , cord-329553-93tbgn2b 198 10 1980 1980 CD cord-329553-93tbgn2b 198 11 . . . cord-329553-93tbgn2b 199 1 The the DT cord-329553-93tbgn2b 199 2 binding binding NN cord-329553-93tbgn2b 199 3 of of IN cord-329553-93tbgn2b 199 4 110-kD 110-kd CD cord-329553-93tbgn2b 199 5 S33D s33d NN cord-329553-93tbgn2b 199 6 to to IN cord-329553-93tbgn2b 199 7 WGA WGA NNP cord-329553-93tbgn2b 199 8 but but CC cord-329553-93tbgn2b 199 9 not not RB cord-329553-93tbgn2b 199 10 sWGA swga JJ cord-329553-93tbgn2b 199 11 not not RB cord-329553-93tbgn2b 199 12 only only RB cord-329553-93tbgn2b 199 13 confirmed confirm VBD cord-329553-93tbgn2b 199 14 the the DT cord-329553-93tbgn2b 199 15 presence presence NN cord-329553-93tbgn2b 199 16 of of IN cord-329553-93tbgn2b 199 17 sialic sialic JJ cord-329553-93tbgn2b 199 18 acid acid NN cord-329553-93tbgn2b 199 19 on on IN cord-329553-93tbgn2b 199 20 the the DT cord-329553-93tbgn2b 199 21 N n NN cord-329553-93tbgn2b 199 22 - - HYPH cord-329553-93tbgn2b 199 23 linked link VBN cord-329553-93tbgn2b 199 24 glycans glycan NNS cord-329553-93tbgn2b 199 25 but but CC cord-329553-93tbgn2b 199 26 also also RB cord-329553-93tbgn2b 199 27 demonstrated demonstrate VBD cord-329553-93tbgn2b 199 28 that that IN cord-329553-93tbgn2b 199 29 no no DT cord-329553-93tbgn2b 199 30 N N NNP cord-329553-93tbgn2b 199 31 - - HYPH cord-329553-93tbgn2b 199 32 acetylglucosamine acetylglucosamine NN cord-329553-93tbgn2b 199 33 residues residue NNS cord-329553-93tbgn2b 199 34 were be VBD cord-329553-93tbgn2b 199 35 exposed expose VBN cord-329553-93tbgn2b 199 36 on on IN cord-329553-93tbgn2b 199 37 the the DT cord-329553-93tbgn2b 199 38 glycans glycan NNS cord-329553-93tbgn2b 199 39 . . . cord-329553-93tbgn2b 200 1 Both both DT cord-329553-93tbgn2b 200 2 the the DT cord-329553-93tbgn2b 200 3 100-and 100-and CD cord-329553-93tbgn2b 200 4 the the DT cord-329553-93tbgn2b 200 5 110-kD 110-kd CD cord-329553-93tbgn2b 200 6 forms form NNS cord-329553-93tbgn2b 200 7 of of IN cord-329553-93tbgn2b 200 8 S33D s33d RB cord-329553-93tbgn2b 200 9 failed fail VBD cord-329553-93tbgn2b 200 10 to to TO cord-329553-93tbgn2b 200 11 bind bind VB cord-329553-93tbgn2b 200 12 Lotusagarose Lotusagarose NNP cord-329553-93tbgn2b 200 13 , , , cord-329553-93tbgn2b 200 14 a a DT cord-329553-93tbgn2b 200 15 fucose fucose JJ cord-329553-93tbgn2b 200 16 - - HYPH cord-329553-93tbgn2b 200 17 specific specific JJ cord-329553-93tbgn2b 200 18 lectin lectin NN cord-329553-93tbgn2b 200 19 . . . cord-329553-93tbgn2b 201 1 This this DT cord-329553-93tbgn2b 201 2 result result NN cord-329553-93tbgn2b 201 3 suggests suggest VBZ cord-329553-93tbgn2b 201 4 that that IN cord-329553-93tbgn2b 201 5 no no DT cord-329553-93tbgn2b 201 6 significant significant JJ cord-329553-93tbgn2b 201 7 fucose fucose JJ cord-329553-93tbgn2b 201 8 residues residue NNS cord-329553-93tbgn2b 201 9 were be VBD cord-329553-93tbgn2b 201 10 added add VBN cord-329553-93tbgn2b 201 11 to to IN cord-329553-93tbgn2b 201 12 the the DT cord-329553-93tbgn2b 201 13 N n NN cord-329553-93tbgn2b 201 14 - - HYPH cord-329553-93tbgn2b 201 15 linked link VBN cord-329553-93tbgn2b 201 16 glycans glycan NNS cord-329553-93tbgn2b 201 17 of of IN cord-329553-93tbgn2b 201 18 S33D. S33D. NNS cord-329553-93tbgn2b 201 19 Similar similar JJ cord-329553-93tbgn2b 201 20 experiments experiment NNS cord-329553-93tbgn2b 201 21 performed perform VBN cord-329553-93tbgn2b 201 22 with with IN cord-329553-93tbgn2b 201 23 cells cell NNS cord-329553-93tbgn2b 201 24 expressing express VBG cord-329553-93tbgn2b 201 25 S64D S64D NNP cord-329553-93tbgn2b 201 26 ( ( -LRB- cord-329553-93tbgn2b 201 27 Fig Fig NNP cord-329553-93tbgn2b 201 28 . . . cord-329553-93tbgn2b 201 29 5 5 CD cord-329553-93tbgn2b 201 30 B b NN cord-329553-93tbgn2b 201 31 ) ) -RRB- cord-329553-93tbgn2b 201 32 revealed reveal VBD cord-329553-93tbgn2b 201 33 that that IN cord-329553-93tbgn2b 201 34 the the DT cord-329553-93tbgn2b 201 35 115-kD 115-kd CD cord-329553-93tbgn2b 201 36 Golgi Golgi NNP cord-329553-93tbgn2b 201 37 form form NN cord-329553-93tbgn2b 201 38 of of IN cord-329553-93tbgn2b 201 39 S64D s64d NN cord-329553-93tbgn2b 201 40 binds bind VBZ cord-329553-93tbgn2b 201 41 Con Con NNP cord-329553-93tbgn2b 201 42 A- A- NNP cord-329553-93tbgn2b 201 43 , , , cord-329553-93tbgn2b 201 44 WGA- WGA- NNP cord-329553-93tbgn2b 201 45 , , , cord-329553-93tbgn2b 201 46 ECA- ECA- NNP cord-329553-93tbgn2b 201 47 , , , cord-329553-93tbgn2b 201 48 SNA SNA NNP cord-329553-93tbgn2b 201 49 , , , cord-329553-93tbgn2b 201 50 and and CC cord-329553-93tbgn2b 201 51 MAA- MAA- NNP cord-329553-93tbgn2b 201 52 , , , cord-329553-93tbgn2b 201 53 but but CC cord-329553-93tbgn2b 201 54 not not RB cord-329553-93tbgn2b 201 55 sWGA swga CD cord-329553-93tbgn2b 201 56 - - : cord-329553-93tbgn2b 201 57 or or CC cord-329553-93tbgn2b 201 58 Lotus Lotus NNP cord-329553-93tbgn2b 201 59 - - HYPH cord-329553-93tbgn2b 201 60 agarose agarose VBN cord-329553-93tbgn2b 201 61 ( ( -LRB- cord-329553-93tbgn2b 201 62 identical identical JJ cord-329553-93tbgn2b 201 63 pattern pattern NN cord-329553-93tbgn2b 201 64 as as IN cord-329553-93tbgn2b 201 65 the the DT cord-329553-93tbgn2b 201 66 110-kD 110-kd CD cord-329553-93tbgn2b 201 67 form form NN cord-329553-93tbgn2b 201 68 of of IN cord-329553-93tbgn2b 201 69 S33D s33d NN cord-329553-93tbgn2b 201 70 ) ) -RRB- cord-329553-93tbgn2b 201 71 . . . cord-329553-93tbgn2b 202 1 The the DT cord-329553-93tbgn2b 202 2 115-kD 115-kd CD cord-329553-93tbgn2b 202 3 form form NN cord-329553-93tbgn2b 202 4 of of IN cord-329553-93tbgn2b 202 5 S64D s64d NN cord-329553-93tbgn2b 202 6 has have VBZ cord-329553-93tbgn2b 202 7 thus thus RB cord-329553-93tbgn2b 202 8 been be VBN cord-329553-93tbgn2b 202 9 modified modify VBN cord-329553-93tbgn2b 202 10 by by IN cord-329553-93tbgn2b 202 11 both both DT cord-329553-93tbgn2b 202 12 GT GT NNP cord-329553-93tbgn2b 202 13 and and CC cord-329553-93tbgn2b 202 14 sialyltransferases sialyltransferase NNS cord-329553-93tbgn2b 202 15 . . . cord-329553-93tbgn2b 203 1 The the DT cord-329553-93tbgn2b 203 2 105-kD 105-kd CD cord-329553-93tbgn2b 203 3 form form NN cord-329553-93tbgn2b 203 4 of of IN cord-329553-93tbgn2b 203 5 S64D s64d NN cord-329553-93tbgn2b 203 6 was be VBD cord-329553-93tbgn2b 203 7 quantitatively quantitatively RB cord-329553-93tbgn2b 203 8 recovered recover VBN cord-329553-93tbgn2b 203 9 from from IN cord-329553-93tbgn2b 203 10 Con Con NNP cord-329553-93tbgn2b 203 11 ( ( -LRB- cord-329553-93tbgn2b 203 12 10041 10041 CD cord-329553-93tbgn2b 203 13 ) ) -RRB- cord-329553-93tbgn2b 203 14 ) ) -RRB- cord-329553-93tbgn2b 203 15 and and CC cord-329553-93tbgn2b 203 16 Golgi Golgi NNP cord-329553-93tbgn2b 203 17 ( ( -LRB- cord-329553-93tbgn2b 203 18 11041 11041 CD cord-329553-93tbgn2b 203 19 ) ) -RRB- cord-329553-93tbgn2b 203 20 ) ) -RRB- cord-329553-93tbgn2b 203 21 forms form NNS cord-329553-93tbgn2b 203 22 were be VBD cord-329553-93tbgn2b 203 23 present present JJ cord-329553-93tbgn2b 203 24 . . . cord-329553-93tbgn2b 204 1 Similar similar JJ cord-329553-93tbgn2b 204 2 amounts amount NNS cord-329553-93tbgn2b 204 3 of of IN cord-329553-93tbgn2b 204 4 the the DT cord-329553-93tbgn2b 204 5 immunocomplex immunocomplex NN cord-329553-93tbgn2b 204 6 were be VBD cord-329553-93tbgn2b 204 7 analyzed analyze VBN cord-329553-93tbgn2b 204 8 directly directly RB cord-329553-93tbgn2b 204 9 ( ( -LRB- cord-329553-93tbgn2b 204 10 Control Control NNP cord-329553-93tbgn2b 204 11 ) ) -RRB- cord-329553-93tbgn2b 204 12 or or CC cord-329553-93tbgn2b 204 13 incubated incubate VBN cord-329553-93tbgn2b 204 14 with with IN cord-329553-93tbgn2b 204 15 various various JJ cord-329553-93tbgn2b 204 16 lectin lectin NN cord-329553-93tbgn2b 204 17 - - HYPH cord-329553-93tbgn2b 204 18 agaroses agarose NNS cord-329553-93tbgn2b 204 19 and and CC cord-329553-93tbgn2b 204 20 the the DT cord-329553-93tbgn2b 204 21 bound bind VBN cord-329553-93tbgn2b 204 22 fraction fraction NN cord-329553-93tbgn2b 204 23 was be VBD cord-329553-93tbgn2b 204 24 then then RB cord-329553-93tbgn2b 204 25 analyzed analyze VBN cord-329553-93tbgn2b 204 26 . . . cord-329553-93tbgn2b 205 1 ( ( -LRB- cord-329553-93tbgn2b 205 2 B b NN cord-329553-93tbgn2b 205 3 ) ) -RRB- cord-329553-93tbgn2b 205 4 Lectin Lectin NNP cord-329553-93tbgn2b 205 5 - - HYPH cord-329553-93tbgn2b 205 6 binding bind VBG cord-329553-93tbgn2b 205 7 properties property NNS cord-329553-93tbgn2b 205 8 of of IN cord-329553-93tbgn2b 205 9 S64D. S64D. NNS cord-329553-93tbgn2b 205 10 Cells cell NNS cord-329553-93tbgn2b 205 11 expressing express VBG cord-329553-93tbgn2b 205 12 S64D S64D NNP cord-329553-93tbgn2b 205 13 were be VBD cord-329553-93tbgn2b 205 14 analyzed analyze VBN cord-329553-93tbgn2b 205 15 as as IN cord-329553-93tbgn2b 205 16 described describe VBN cord-329553-93tbgn2b 205 17 for for IN cord-329553-93tbgn2b 205 18 S331 S331 NNP cord-329553-93tbgn2b 205 19 ) ) -RRB- cord-329553-93tbgn2b 205 20 . . . cord-329553-93tbgn2b 206 1 Con Con NNP cord-329553-93tbgn2b 206 2 A A NNP cord-329553-93tbgn2b 206 3 , , , cord-329553-93tbgn2b 206 4 concanavalin concanavalin NNP cord-329553-93tbgn2b 206 5 A a NN cord-329553-93tbgn2b 206 6 ; ; : cord-329553-93tbgn2b 206 7 sWGA sWGA NNP cord-329553-93tbgn2b 206 8 , , , cord-329553-93tbgn2b 206 9 succinylated succinylated JJ cord-329553-93tbgn2b 206 10 wheat wheat NN cord-329553-93tbgn2b 206 11 germ germ NNP cord-329553-93tbgn2b 206 12 agglutinin agglutinin NNP cord-329553-93tbgn2b 206 13 ; ; : cord-329553-93tbgn2b 206 14 WGA WGA NNP cord-329553-93tbgn2b 206 15 , , , cord-329553-93tbgn2b 206 16 wheat wheat NN cord-329553-93tbgn2b 206 17 germ germ NN cord-329553-93tbgn2b 206 18 agglutinin agglutinin NNS cord-329553-93tbgn2b 206 19 ; ; : cord-329553-93tbgn2b 206 20 ECA ECA NNP cord-329553-93tbgn2b 206 21 , , , cord-329553-93tbgn2b 206 22 Erythrina Erythrina NNP cord-329553-93tbgn2b 206 23 cristagalli cristagalli NN cord-329553-93tbgn2b 206 24 ; ; : cord-329553-93tbgn2b 206 25 Lotus Lotus NNP cord-329553-93tbgn2b 206 26 , , , cord-329553-93tbgn2b 206 27 Lotus Lotus NNP cord-329553-93tbgn2b 206 28 tetragonolobus tetragonolobus NN cord-329553-93tbgn2b 206 29 ; ; : cord-329553-93tbgn2b 206 30 SNA SNA NNP cord-329553-93tbgn2b 206 31 , , , cord-329553-93tbgn2b 206 32 Sambucus Sambucus NNP cord-329553-93tbgn2b 206 33 nigra nigra NN cord-329553-93tbgn2b 206 34 ; ; : cord-329553-93tbgn2b 206 35 MAA MAA NNP cord-329553-93tbgn2b 206 36 , , , cord-329553-93tbgn2b 206 37 Maackia Maackia NNP cord-329553-93tbgn2b 206 38 amurensis amurensis NN cord-329553-93tbgn2b 206 39 . . . cord-329553-93tbgn2b 207 1 A a DT cord-329553-93tbgn2b 207 2 - - HYPH cord-329553-93tbgn2b 207 3 agarose agarose JJ cord-329553-93tbgn2b 207 4 beads bead NNS cord-329553-93tbgn2b 207 5 . . . cord-329553-93tbgn2b 208 1 Interestingly interestingly RB cord-329553-93tbgn2b 208 2 , , , cord-329553-93tbgn2b 208 3 a a DT cord-329553-93tbgn2b 208 4 small small JJ cord-329553-93tbgn2b 208 5 but but CC cord-329553-93tbgn2b 208 6 detectable detectable JJ cord-329553-93tbgn2b 208 7 portion portion NN cord-329553-93tbgn2b 208 8 of of IN cord-329553-93tbgn2b 208 9 the the DT cord-329553-93tbgn2b 208 10 105-kD 105-kd CD cord-329553-93tbgn2b 208 11 form form NN cord-329553-93tbgn2b 208 12 of of IN cord-329553-93tbgn2b 208 13 S64D s64d NN cord-329553-93tbgn2b 208 14 was be VBD cord-329553-93tbgn2b 208 15 also also RB cord-329553-93tbgn2b 208 16 capable capable JJ cord-329553-93tbgn2b 208 17 of of IN cord-329553-93tbgn2b 208 18 binding bind VBG cord-329553-93tbgn2b 208 19 WGA WGA NNP cord-329553-93tbgn2b 208 20 , , , cord-329553-93tbgn2b 208 21 ECA ECA NNP cord-329553-93tbgn2b 208 22 , , , cord-329553-93tbgn2b 208 23 and and CC cord-329553-93tbgn2b 208 24 SNA SNA NNP cord-329553-93tbgn2b 208 25 ( ( -LRB- cord-329553-93tbgn2b 208 26 but but CC cord-329553-93tbgn2b 208 27 not not RB cord-329553-93tbgn2b 208 28 sWGA swga JJ cord-329553-93tbgn2b 208 29 , , , cord-329553-93tbgn2b 208 30 Lotus Lotus NNP cord-329553-93tbgn2b 208 31 , , , cord-329553-93tbgn2b 208 32 or or CC cord-329553-93tbgn2b 208 33 MAA MAA NNP cord-329553-93tbgn2b 208 34 ) ) -RRB- cord-329553-93tbgn2b 208 35 . . . cord-329553-93tbgn2b 209 1 This this DT cord-329553-93tbgn2b 209 2 observation observation NN cord-329553-93tbgn2b 209 3 suggests suggest VBZ cord-329553-93tbgn2b 209 4 that that IN cord-329553-93tbgn2b 209 5 a a DT cord-329553-93tbgn2b 209 6 small small JJ cord-329553-93tbgn2b 209 7 fraction fraction NN cord-329553-93tbgn2b 209 8 of of IN cord-329553-93tbgn2b 209 9 the the DT cord-329553-93tbgn2b 209 10 105-kD 105-kd CD cord-329553-93tbgn2b 209 11 form form NN cord-329553-93tbgn2b 209 12 might may MD cord-329553-93tbgn2b 209 13 have have VB cord-329553-93tbgn2b 209 14 undergone undergo VBN cord-329553-93tbgn2b 209 15 modifications modification NNS cord-329553-93tbgn2b 209 16 by by IN cord-329553-93tbgn2b 209 17 0-GT 0-gt CD cord-329553-93tbgn2b 209 18 and and CC cord-329553-93tbgn2b 209 19 ST ST NNP cord-329553-93tbgn2b 209 20 , , , cord-329553-93tbgn2b 209 21 although although IN cord-329553-93tbgn2b 209 22 the the DT cord-329553-93tbgn2b 209 23 possibility possibility NN cord-329553-93tbgn2b 209 24 that that IN cord-329553-93tbgn2b 209 25 the the DT cord-329553-93tbgn2b 209 26 observed observed JJ cord-329553-93tbgn2b 209 27 binding binding NN cord-329553-93tbgn2b 209 28 of of IN cord-329553-93tbgn2b 209 29 the the DT cord-329553-93tbgn2b 209 30 105-kD 105-kd CD cord-329553-93tbgn2b 209 31 form form NN cord-329553-93tbgn2b 209 32 of of IN cord-329553-93tbgn2b 209 33 S64D s64d NN cord-329553-93tbgn2b 209 34 to to IN cord-329553-93tbgn2b 209 35 WGA WGA NNP cord-329553-93tbgn2b 209 36 , , , cord-329553-93tbgn2b 209 37 ECA ECA NNP cord-329553-93tbgn2b 209 38 , , , cord-329553-93tbgn2b 209 39 and and CC cord-329553-93tbgn2b 209 40 SNA SNA NNP cord-329553-93tbgn2b 209 41 was be VBD cord-329553-93tbgn2b 209 42 due due JJ cord-329553-93tbgn2b 209 43 to to IN cord-329553-93tbgn2b 209 44 nonspecific nonspecific JJ cord-329553-93tbgn2b 209 45 background background NN cord-329553-93tbgn2b 209 46 binding binding NN cord-329553-93tbgn2b 209 47 could could MD cord-329553-93tbgn2b 209 48 not not RB cord-329553-93tbgn2b 209 49 be be VB cord-329553-93tbgn2b 209 50 excluded exclude VBN cord-329553-93tbgn2b 209 51 yet yet RB cord-329553-93tbgn2b 209 52 . . . cord-329553-93tbgn2b 210 1 The the DT cord-329553-93tbgn2b 210 2 small small JJ cord-329553-93tbgn2b 210 3 amount amount NN cord-329553-93tbgn2b 210 4 of of IN cord-329553-93tbgn2b 210 5 105-kD 105-kd CD cord-329553-93tbgn2b 210 6 form form NN cord-329553-93tbgn2b 210 7 present present JJ cord-329553-93tbgn2b 210 8 in in IN cord-329553-93tbgn2b 210 9 pulse pulse NN cord-329553-93tbgn2b 210 10 - - HYPH cord-329553-93tbgn2b 210 11 labeled label VBN cord-329553-93tbgn2b 210 12 cells cell NNS cord-329553-93tbgn2b 210 13 after after IN cord-329553-93tbgn2b 210 14 90 90 CD cord-329553-93tbgn2b 210 15 min min NN cord-329553-93tbgn2b 210 16 of of IN cord-329553-93tbgn2b 210 17 chase chase NNP cord-329553-93tbgn2b 210 18 ( ( -LRB- cord-329553-93tbgn2b 210 19 Fig Fig NNP cord-329553-93tbgn2b 210 20 . . . cord-329553-93tbgn2b 210 21 3 3 CD cord-329553-93tbgn2b 210 22 A A NNP cord-329553-93tbgn2b 210 23 , , , cord-329553-93tbgn2b 210 24 lane lane NN cord-329553-93tbgn2b 210 25 5 5 CD cord-329553-93tbgn2b 210 26 ) ) -RRB- cord-329553-93tbgn2b 210 27 may may MD cord-329553-93tbgn2b 210 28 therefore therefore RB cord-329553-93tbgn2b 210 29 have have VB cord-329553-93tbgn2b 210 30 been be VBN cord-329553-93tbgn2b 210 31 transported transport VBN cord-329553-93tbgn2b 210 32 to to IN cord-329553-93tbgn2b 210 33 the the DT cord-329553-93tbgn2b 210 34 Golgi Golgi NNP cord-329553-93tbgn2b 210 35 apparatus apparatus NN cord-329553-93tbgn2b 210 36 . . . cord-329553-93tbgn2b 211 1 These these DT cord-329553-93tbgn2b 211 2 results result NNS cord-329553-93tbgn2b 211 3 , , , cord-329553-93tbgn2b 211 4 taken take VBN cord-329553-93tbgn2b 211 5 together together RB cord-329553-93tbgn2b 211 6 , , , cord-329553-93tbgn2b 211 7 suggest suggest VBP cord-329553-93tbgn2b 211 8 strongly strongly RB cord-329553-93tbgn2b 211 9 that that IN cord-329553-93tbgn2b 211 10 both both CC cord-329553-93tbgn2b 211 11 S33D s33d NN cord-329553-93tbgn2b 211 12 and and CC cord-329553-93tbgn2b 211 13 S64D s64d NN cord-329553-93tbgn2b 211 14 are be VBP cord-329553-93tbgn2b 211 15 localized localize VBN cord-329553-93tbgn2b 211 16 in in IN cord-329553-93tbgn2b 211 17 the the DT cord-329553-93tbgn2b 211 18 trans trans NNP cord-329553-93tbgn2b 211 19 - - NNP cord-329553-93tbgn2b 211 20 Golgi Golgi NNP cord-329553-93tbgn2b 211 21 cisternae cisternae NNP cord-329553-93tbgn2b 211 22 and/or and/or CC cord-329553-93tbgn2b 211 23 TGN TGN NNP cord-329553-93tbgn2b 211 24 . . . cord-329553-93tbgn2b 212 1 We -PRON- PRP cord-329553-93tbgn2b 212 2 have have VBP cord-329553-93tbgn2b 212 3 thus thus RB cord-329553-93tbgn2b 212 4 demonstrated demonstrate VBN cord-329553-93tbgn2b 212 5 that that IN cord-329553-93tbgn2b 212 6 both both CC cord-329553-93tbgn2b 212 7 S33D s33d NN cord-329553-93tbgn2b 212 8 and and CC cord-329553-93tbgn2b 212 9 S64D s64d NN cord-329553-93tbgn2b 212 10 are be VBP cord-329553-93tbgn2b 212 11 predominantly predominantly RB cord-329553-93tbgn2b 212 12 present present JJ cord-329553-93tbgn2b 212 13 in in IN cord-329553-93tbgn2b 212 14 the the DT cord-329553-93tbgn2b 212 15 Golgi Golgi NNP cord-329553-93tbgn2b 212 16 apparatus apparatus NN cord-329553-93tbgn2b 212 17 . . . cord-329553-93tbgn2b 213 1 Similar similar JJ cord-329553-93tbgn2b 213 2 results result NNS cord-329553-93tbgn2b 213 3 were be VBD cord-329553-93tbgn2b 213 4 obtained obtain VBN cord-329553-93tbgn2b 213 5 for for IN cord-329553-93tbgn2b 213 6 S51D s51d NN cord-329553-93tbgn2b 213 7 , , , cord-329553-93tbgn2b 213 8 S46D S46D NNP cord-329553-93tbgn2b 213 9 , , , cord-329553-93tbgn2b 213 10 and and CC cord-329553-93tbgn2b 213 11 S40D s40d NN cord-329553-93tbgn2b 213 12 ( ( -LRB- cord-329553-93tbgn2b 213 13 data datum NNS cord-329553-93tbgn2b 213 14 not not RB cord-329553-93tbgn2b 213 15 shown show VBN cord-329553-93tbgn2b 213 16 ) ) -RRB- cord-329553-93tbgn2b 213 17 . . . cord-329553-93tbgn2b 214 1 These these DT cord-329553-93tbgn2b 214 2 results result NNS cord-329553-93tbgn2b 214 3 demonstrate demonstrate VBP cord-329553-93tbgn2b 214 4 that that IN cord-329553-93tbgn2b 214 5 the the DT cord-329553-93tbgn2b 214 6 NHz nhz NN cord-329553-93tbgn2b 214 7 - - HYPH cord-329553-93tbgn2b 214 8 terminal terminal JJ cord-329553-93tbgn2b 214 9 33-residue 33-residue CD cord-329553-93tbgn2b 214 10 sequence sequence NN cord-329553-93tbgn2b 214 11 of of IN cord-329553-93tbgn2b 214 12 ST ST NNP cord-329553-93tbgn2b 214 13 is be VBZ cord-329553-93tbgn2b 214 14 sufficient sufficient JJ cord-329553-93tbgn2b 214 15 for for IN cord-329553-93tbgn2b 214 16 Golgi Golgi NNP cord-329553-93tbgn2b 214 17 localization localization NN cord-329553-93tbgn2b 214 18 and and CC cord-329553-93tbgn2b 214 19 that that IN cord-329553-93tbgn2b 214 20 the the DT cord-329553-93tbgn2b 214 21 stem stem NN cord-329553-93tbgn2b 214 22 region region NN cord-329553-93tbgn2b 214 23 of of IN cord-329553-93tbgn2b 214 24 ST ST NNP cord-329553-93tbgn2b 214 25 ( ( -LRB- cord-329553-93tbgn2b 214 26 sequence sequence NN cord-329553-93tbgn2b 214 27 from from IN cord-329553-93tbgn2b 214 28 residue residue NN cord-329553-93tbgn2b 214 29 34 34 CD cord-329553-93tbgn2b 214 30 to to IN cord-329553-93tbgn2b 214 31 63 63 CD cord-329553-93tbgn2b 214 32 ) ) -RRB- cord-329553-93tbgn2b 214 33 is be VBZ cord-329553-93tbgn2b 214 34 apparently apparently RB cord-329553-93tbgn2b 214 35 not not RB cord-329553-93tbgn2b 214 36 essential essential JJ cord-329553-93tbgn2b 214 37 for for IN cord-329553-93tbgn2b 214 38 Golgi Golgi NNP cord-329553-93tbgn2b 214 39 localization localization NN cord-329553-93tbgn2b 214 40 . . . cord-329553-93tbgn2b 215 1 The the DT cord-329553-93tbgn2b 215 2 sequence sequence NN cord-329553-93tbgn2b 215 3 in in IN cord-329553-93tbgn2b 215 4 this this DT cord-329553-93tbgn2b 215 5 stem stem NN cord-329553-93tbgn2b 215 6 region region NN cord-329553-93tbgn2b 215 7 is be VBZ cord-329553-93tbgn2b 215 8 in in IN cord-329553-93tbgn2b 215 9 fact fact NN cord-329553-93tbgn2b 215 10 the the DT cord-329553-93tbgn2b 215 11 most most RBS cord-329553-93tbgn2b 215 12 divergent divergent JJ cord-329553-93tbgn2b 215 13 be-25 be-25 NNP cord-329553-93tbgn2b 215 14 1 1 CD cord-329553-93tbgn2b 215 15 tween tween NN cord-329553-93tbgn2b 215 16 rat rat NN cord-329553-93tbgn2b 215 17 and and CC cord-329553-93tbgn2b 215 18 human human JJ cord-329553-93tbgn2b 215 19 ST ST NNP cord-329553-93tbgn2b 215 20 . . . cord-329553-93tbgn2b 216 1 The the DT cord-329553-93tbgn2b 216 2 NH2-terminal nh2-terminal NN cord-329553-93tbgn2b 216 3 64-residue 64-residue CD cord-329553-93tbgn2b 216 4 sequence sequence NN cord-329553-93tbgn2b 216 5 ofhuman ofhuman NNP cord-329553-93tbgn2b 216 6 ST ST NNP cord-329553-93tbgn2b 217 1 ( ( -LRB- cord-329553-93tbgn2b 217 2 Grundmann Grundmann NNP cord-329553-93tbgn2b 217 3 et et NNP cord-329553-93tbgn2b 217 4 al al NNP cord-329553-93tbgn2b 217 5 . . NNP cord-329553-93tbgn2b 217 6 , , , cord-329553-93tbgn2b 217 7 1990 1990 CD cord-329553-93tbgn2b 217 8 ) ) -RRB- cord-329553-93tbgn2b 217 9 is be VBZ cord-329553-93tbgn2b 217 10 also also RB cord-329553-93tbgn2b 217 11 shown show VBN cord-329553-93tbgn2b 217 12 in in IN cord-329553-93tbgn2b 217 13 Fig Fig NNP cord-329553-93tbgn2b 217 14 . . . cord-329553-93tbgn2b 218 1 1 1 LS cord-329553-93tbgn2b 218 2 A a NN cord-329553-93tbgn2b 218 3 ( ( -LRB- cord-329553-93tbgn2b 218 4 M M NNP cord-329553-93tbgn2b 218 5 ) ) -RRB- cord-329553-93tbgn2b 218 6 . . . cord-329553-93tbgn2b 219 1 Incontrast Incontrast NNP cord-329553-93tbgn2b 219 2 to to IN cord-329553-93tbgn2b 219 3 the the DT cord-329553-93tbgn2b 219 4 stem stem NN cord-329553-93tbgn2b 219 5 region region NN cord-329553-93tbgn2b 219 6 , , , cord-329553-93tbgn2b 219 7 the the DT cord-329553-93tbgn2b 219 8 sequence sequence NN cord-329553-93tbgn2b 219 9 of of IN cord-329553-93tbgn2b 219 10 the the DT cord-329553-93tbgn2b 219 11 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 219 12 domain domain NN cord-329553-93tbgn2b 219 13 is be VBZ cord-329553-93tbgn2b 219 14 totally totally RB cord-329553-93tbgn2b 219 15 conserved conserve VBN cord-329553-93tbgn2b 219 16 between between IN cord-329553-93tbgn2b 219 17 the the DT cord-329553-93tbgn2b 219 18 rat rat NN cord-329553-93tbgn2b 219 19 and and CC cord-329553-93tbgn2b 219 20 human human JJ cord-329553-93tbgn2b 219 21 ST ST NNP cord-329553-93tbgn2b 219 22 . . . cord-329553-93tbgn2b 220 1 The the DT cord-329553-93tbgn2b 220 2 transmembrane transmembrane NN cord-329553-93tbgn2b 220 3 domain domain NN cord-329553-93tbgn2b 220 4 is be VBZ cord-329553-93tbgn2b 220 5 also also RB cord-329553-93tbgn2b 220 6 highly highly RB cord-329553-93tbgn2b 220 7 conserved conserve VBN cord-329553-93tbgn2b 220 8 . . . cord-329553-93tbgn2b 221 1 The the DT cord-329553-93tbgn2b 221 2 sequence sequence NN cord-329553-93tbgn2b 221 3 conservation conservation NN cord-329553-93tbgn2b 221 4 of of IN cord-329553-93tbgn2b 221 5 the the DT cord-329553-93tbgn2b 221 6 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 221 7 and and CC cord-329553-93tbgn2b 221 8 transmembrane transmembrane NN cord-329553-93tbgn2b 221 9 domains domain NNS cord-329553-93tbgn2b 221 10 but but CC cord-329553-93tbgn2b 221 11 not not RB cord-329553-93tbgn2b 221 12 the the DT cord-329553-93tbgn2b 221 13 stem stem NN cord-329553-93tbgn2b 221 14 region region NN cord-329553-93tbgn2b 221 15 is be VBZ cord-329553-93tbgn2b 221 16 consistent consistent JJ cord-329553-93tbgn2b 221 17 with with IN cord-329553-93tbgn2b 221 18 our -PRON- PRP$ cord-329553-93tbgn2b 221 19 finding finding NN cord-329553-93tbgn2b 221 20 that that IN cord-329553-93tbgn2b 221 21 the the DT cord-329553-93tbgn2b 221 22 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 221 23 33-residue 33-residue CD cord-329553-93tbgn2b 221 24 sequence sequence NN cord-329553-93tbgn2b 221 25 of of IN cord-329553-93tbgn2b 221 26 ST ST NNP cord-329553-93tbgn2b 221 27 is be VBZ cord-329553-93tbgn2b 221 28 sufficient sufficient JJ cord-329553-93tbgn2b 221 29 for for IN cord-329553-93tbgn2b 221 30 Golgi Golgi NNP cord-329553-93tbgn2b 221 31 localization localization NN cord-329553-93tbgn2b 221 32 . . . cord-329553-93tbgn2b 222 1 This this DT cord-329553-93tbgn2b 222 2 33residue 33residue JJ cord-329553-93tbgn2b 222 3 sequence sequence NN cord-329553-93tbgn2b 222 4 can can MD cord-329553-93tbgn2b 222 5 be be VB cord-329553-93tbgn2b 222 6 further further RB cord-329553-93tbgn2b 222 7 divided divide VBN cord-329553-93tbgn2b 222 8 into into IN cord-329553-93tbgn2b 222 9 three three CD cord-329553-93tbgn2b 222 10 regions region NNS cord-329553-93tbgn2b 222 11 : : : cord-329553-93tbgn2b 222 12 the the DT cord-329553-93tbgn2b 222 13 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 222 14 domain domain NN cord-329553-93tbgn2b 222 15 , , , cord-329553-93tbgn2b 222 16 the the DT cord-329553-93tbgn2b 222 17 transmembrane transmembrane NN cord-329553-93tbgn2b 222 18 domain domain NN cord-329553-93tbgn2b 222 19 , , , cord-329553-93tbgn2b 222 20 and and CC cord-329553-93tbgn2b 222 21 the the DT cord-329553-93tbgn2b 222 22 7-residue 7-residue CD cord-329553-93tbgn2b 222 23 sequence sequence NN cord-329553-93tbgn2b 222 24 flanking flank VBG cord-329553-93tbgn2b 222 25 the the DT cord-329553-93tbgn2b 222 26 COOH COOH NNP cord-329553-93tbgn2b 222 27 - - HYPH cord-329553-93tbgn2b 222 28 terminal terminal JJ cord-329553-93tbgn2b 222 29 side side NN cord-329553-93tbgn2b 222 30 of of IN cord-329553-93tbgn2b 222 31 the the DT cord-329553-93tbgn2b 222 32 transmembrane transmembrane NN cord-329553-93tbgn2b 222 33 domain domain NN cord-329553-93tbgn2b 222 34 . . . cord-329553-93tbgn2b 223 1 To to TO cord-329553-93tbgn2b 223 2 further further RB cord-329553-93tbgn2b 223 3 narrow narrow VB cord-329553-93tbgn2b 223 4 down down RP cord-329553-93tbgn2b 223 5 the the DT cord-329553-93tbgn2b 223 6 sequence sequence NN cord-329553-93tbgn2b 223 7 required require VBN cord-329553-93tbgn2b 223 8 for for IN cord-329553-93tbgn2b 223 9 Golgi Golgi NNP cord-329553-93tbgn2b 223 10 localization localization NN cord-329553-93tbgn2b 223 11 , , , cord-329553-93tbgn2b 223 12 an an DT cord-329553-93tbgn2b 223 13 additional additional JJ cord-329553-93tbgn2b 223 14 five five CD cord-329553-93tbgn2b 223 15 chimeric chimeric JJ cord-329553-93tbgn2b 223 16 cDNAs cdna NNS cord-329553-93tbgn2b 223 17 were be VBD cord-329553-93tbgn2b 223 18 constructed construct VBN cord-329553-93tbgn2b 223 19 which which WDT cord-329553-93tbgn2b 223 20 encode encode VBP cord-329553-93tbgn2b 223 21 fusion fusion NN cord-329553-93tbgn2b 223 22 proteins protein NNS cord-329553-93tbgn2b 223 23 S26D s26d VBP cord-329553-93tbgn2b 223 24 , , , cord-329553-93tbgn2b 223 25 S9D s9d NN cord-329553-93tbgn2b 223 26 , , , cord-329553-93tbgn2b 223 27 S10 S10 NNP cord-329553-93tbgn2b 223 28 - - HYPH cord-329553-93tbgn2b 223 29 33D 33d NN cord-329553-93tbgn2b 223 30 , , , cord-329553-93tbgn2b 223 31 S10 S10 NNP cord-329553-93tbgn2b 223 32 - - HYPH cord-329553-93tbgn2b 223 33 26D 26d NN cord-329553-93tbgn2b 223 34 , , , cord-329553-93tbgn2b 223 35 and and CC cord-329553-93tbgn2b 223 36 S27 S27 NNP cord-329553-93tbgn2b 223 37 - - HYPH cord-329553-93tbgn2b 223 38 33D 33D NNP cord-329553-93tbgn2b 223 39 ( ( -LRB- cord-329553-93tbgn2b 223 40 Fig fig NN cord-329553-93tbgn2b 223 41 . . . cord-329553-93tbgn2b 224 1 1 1 CD cord-329553-93tbgn2b 224 2 _SP cord-329553-93tbgn2b 224 3 B B NNP cord-329553-93tbgn2b 224 4 ) ) -RRB- cord-329553-93tbgn2b 224 5 . . . cord-329553-93tbgn2b 225 1 In in IN cord-329553-93tbgn2b 225 2 vitro vitro FW cord-329553-93tbgn2b 225 3 translation translation NN cord-329553-93tbgn2b 225 4 studies study NNS cord-329553-93tbgn2b 225 5 demonstrated demonstrate VBD cord-329553-93tbgn2b 225 6 that that IN cord-329553-93tbgn2b 225 7 S26D s26d NN cord-329553-93tbgn2b 225 8 , , , cord-329553-93tbgn2b 225 9 like like IN cord-329553-93tbgn2b 225 10 S27D s27d NN cord-329553-93tbgn2b 225 11 , , , cord-329553-93tbgn2b 225 12 was be VBD cord-329553-93tbgn2b 225 13 not not RB cord-329553-93tbgn2b 225 14 anchored anchor VBN cord-329553-93tbgn2b 225 15 in in IN cord-329553-93tbgn2b 225 16 the the DT cord-329553-93tbgn2b 225 17 microsomal microsomal NNP cord-329553-93tbgn2b 225 18 membrane membrane NN cord-329553-93tbgn2b 225 19 ( ( -LRB- cord-329553-93tbgn2b 225 20 Fig Fig NNP cord-329553-93tbgn2b 225 21 . . . cord-329553-93tbgn2b 225 22 _SP cord-329553-93tbgn2b 225 23 2 2 CD cord-329553-93tbgn2b 225 24 ) ) -RRB- cord-329553-93tbgn2b 225 25 . . . cord-329553-93tbgn2b 226 1 Interestingly interestingly RB cord-329553-93tbgn2b 226 2 , , , cord-329553-93tbgn2b 226 3 S10 S10 NNP cord-329553-93tbgn2b 226 4 - - HYPH cord-329553-93tbgn2b 226 5 26D 26d NN cord-329553-93tbgn2b 226 6 , , , cord-329553-93tbgn2b 226 7 which which WDT cord-329553-93tbgn2b 226 8 is be VBZ cord-329553-93tbgn2b 226 9 identical identical JJ cord-329553-93tbgn2b 226 10 to to TO cord-329553-93tbgn2b 226 11 S26D s26d VB cord-329553-93tbgn2b 226 12 except except IN cord-329553-93tbgn2b 226 13 that that IN cord-329553-93tbgn2b 226 14 the the DT cord-329553-93tbgn2b 226 15 ST ST NNP cord-329553-93tbgn2b 226 16 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 226 17 domain domain NN cord-329553-93tbgn2b 226 18 was be VBD cord-329553-93tbgn2b 226 19 replaced replace VBN cord-329553-93tbgn2b 226 20 by by IN cord-329553-93tbgn2b 226 21 that that DT cord-329553-93tbgn2b 226 22 of of IN cord-329553-93tbgn2b 226 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 226 24 , , , cord-329553-93tbgn2b 226 25 was be VBD cord-329553-93tbgn2b 226 26 mainly mainly RB cord-329553-93tbgn2b 226 27 ( ( -LRB- cord-329553-93tbgn2b 226 28 N70 N70 NNP cord-329553-93tbgn2b 226 29 % % NN cord-329553-93tbgn2b 226 30 ) ) -RRB- cord-329553-93tbgn2b 226 31 anchored anchor VBN cord-329553-93tbgn2b 226 32 in in IN cord-329553-93tbgn2b 226 33 the the DT cord-329553-93tbgn2b 226 34 membrane membrane NN cord-329553-93tbgn2b 226 35 ( ( -LRB- cord-329553-93tbgn2b 226 36 Fig Fig NNP cord-329553-93tbgn2b 226 37 . . NNP cord-329553-93tbgn2b 226 38 2 2 CD cord-329553-93tbgn2b 226 39 ) ) -RRB- cord-329553-93tbgn2b 226 40 . . . cord-329553-93tbgn2b 227 1 For for IN cord-329553-93tbgn2b 227 2 S26D s26d NN cord-329553-93tbgn2b 227 3 , , , cord-329553-93tbgn2b 227 4 S27D s27d NN cord-329553-93tbgn2b 227 5 , , , cord-329553-93tbgn2b 227 6 and and CC cord-329553-93tbgn2b 227 7 S10 S10 NNP cord-329553-93tbgn2b 227 8 - - HYPH cord-329553-93tbgn2b 227 9 26D 26d NN cord-329553-93tbgn2b 227 10 , , , cord-329553-93tbgn2b 227 11 the the DT cord-329553-93tbgn2b 227 12 translocated translocate VBN cord-329553-93tbgn2b 227 13 form form NN cord-329553-93tbgn2b 227 14 in in IN cord-329553-93tbgn2b 227 15 the the DT cord-329553-93tbgn2b 227 16 supernatant supernatant NN cord-329553-93tbgn2b 227 17 was be VBD cord-329553-93tbgn2b 227 18 ofa ofa RB cord-329553-93tbgn2b 227 19 smaller small JJR cord-329553-93tbgn2b 227 20 size size NN cord-329553-93tbgn2b 227 21 than than IN cord-329553-93tbgn2b 227 22 the the DT cord-329553-93tbgn2b 227 23 translocated translocate VBN cord-329553-93tbgn2b 227 24 form form NN cord-329553-93tbgn2b 227 25 in in IN cord-329553-93tbgn2b 227 26 the the DT cord-329553-93tbgn2b 227 27 membrane membrane NN cord-329553-93tbgn2b 227 28 , , , cord-329553-93tbgn2b 227 29 implying imply VBG cord-329553-93tbgn2b 227 30 that that IN cord-329553-93tbgn2b 227 31 the the DT cord-329553-93tbgn2b 227 32 nonanchorage nonanchorage NN cord-329553-93tbgn2b 227 33 in in IN cord-329553-93tbgn2b 227 34 the the DT cord-329553-93tbgn2b 227 35 membrane membrane NN cord-329553-93tbgn2b 227 36 of of IN cord-329553-93tbgn2b 227 37 S26D s26d NN cord-329553-93tbgn2b 227 38 , , , cord-329553-93tbgn2b 227 39 S27D s27d NN cord-329553-93tbgn2b 227 40 , , , cord-329553-93tbgn2b 227 41 and and CC cord-329553-93tbgn2b 227 42 , , , cord-329553-93tbgn2b 227 43 to to IN cord-329553-93tbgn2b 227 44 a a DT cord-329553-93tbgn2b 227 45 much much RB cord-329553-93tbgn2b 227 46 lesser less JJR cord-329553-93tbgn2b 227 47 extent extent NN cord-329553-93tbgn2b 227 48 , , , cord-329553-93tbgn2b 227 49 SIO-26D sio-26d NN cord-329553-93tbgn2b 227 50 might may MD cord-329553-93tbgn2b 227 51 be be VB cord-329553-93tbgn2b 227 52 due due JJ cord-329553-93tbgn2b 227 53 to to IN cord-329553-93tbgn2b 227 54 a a DT cord-329553-93tbgn2b 227 55 cleavage cleavage NN cord-329553-93tbgn2b 227 56 of of IN cord-329553-93tbgn2b 227 57 ( ( -LRB- cord-329553-93tbgn2b 227 58 or or CC cord-329553-93tbgn2b 227 59 part part NN cord-329553-93tbgn2b 227 60 of of IN cord-329553-93tbgn2b 227 61 ) ) -RRB- cord-329553-93tbgn2b 227 62 the the DT cord-329553-93tbgn2b 227 63 signal signal NN cord-329553-93tbgn2b 227 64 / / SYM cord-329553-93tbgn2b 227 65 anchor anchor NN cord-329553-93tbgn2b 227 66 sequence sequence NN cord-329553-93tbgn2b 227 67 ofST ofst JJ cord-329553-93tbgn2b 227 68 by by IN cord-329553-93tbgn2b 227 69 the the DT cord-329553-93tbgn2b 227 70 signal signal NN cord-329553-93tbgn2b 227 71 peptidase peptidase NN cord-329553-93tbgn2b 227 72 , , , cord-329553-93tbgn2b 227 73 although although IN cord-329553-93tbgn2b 227 74 this this DT cord-329553-93tbgn2b 227 75 explanation explanation NN cord-329553-93tbgn2b 227 76 will will MD cord-329553-93tbgn2b 227 77 have have VB cord-329553-93tbgn2b 227 78 to to TO cord-329553-93tbgn2b 227 79 be be VB cord-329553-93tbgn2b 227 80 verified verify VBN cord-329553-93tbgn2b 227 81 by by IN cord-329553-93tbgn2b 227 82 future future JJ cord-329553-93tbgn2b 227 83 experiments experiment NNS cord-329553-93tbgn2b 227 84 . . . cord-329553-93tbgn2b 228 1 Replacing replace VBG cord-329553-93tbgn2b 228 2 the the DT cord-329553-93tbgn2b 228 3 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 228 4 domain domain NN cord-329553-93tbgn2b 228 5 of of IN cord-329553-93tbgn2b 228 6 ST ST NNP cord-329553-93tbgn2b 228 7 by by IN cord-329553-93tbgn2b 228 8 that that DT cord-329553-93tbgn2b 228 9 of of IN cord-329553-93tbgn2b 228 10 DPPIV DPPIV NNP cord-329553-93tbgn2b 228 11 in in IN cord-329553-93tbgn2b 228 12 S10 S10 NNP cord-329553-93tbgn2b 228 13 - - HYPH cord-329553-93tbgn2b 228 14 26D 26d NN cord-329553-93tbgn2b 228 15 significantly significantly RB cord-329553-93tbgn2b 228 16 enhanced enhance VBD cord-329553-93tbgn2b 228 17 the the DT cord-329553-93tbgn2b 228 18 membrane membrane NN cord-329553-93tbgn2b 228 19 anchorage anchorage NN cord-329553-93tbgn2b 228 20 . . . cord-329553-93tbgn2b 229 1 In in IN cord-329553-93tbgn2b 229 2 connection connection NN cord-329553-93tbgn2b 229 3 with with IN cord-329553-93tbgn2b 229 4 the the DT cord-329553-93tbgn2b 229 5 results result NNS cord-329553-93tbgn2b 229 6 obtained obtain VBN cord-329553-93tbgn2b 229 7 with with IN cord-329553-93tbgn2b 229 8 S27D s27d NN cord-329553-93tbgn2b 229 9 and and CC cord-329553-93tbgn2b 229 10 S33D s33d NN cord-329553-93tbgn2b 230 1 ( ( -LRB- cord-329553-93tbgn2b 230 2 Fig Fig NNP cord-329553-93tbgn2b 230 3 . . NNP cord-329553-93tbgn2b 230 4 2 2 CD cord-329553-93tbgn2b 230 5 ) ) -RRB- cord-329553-93tbgn2b 230 6 , , , cord-329553-93tbgn2b 230 7 it -PRON- PRP cord-329553-93tbgn2b 230 8 can can MD cord-329553-93tbgn2b 230 9 be be VB cord-329553-93tbgn2b 230 10 concluded conclude VBN cord-329553-93tbgn2b 230 11 that that IN cord-329553-93tbgn2b 230 12 the the DT cord-329553-93tbgn2b 230 13 signal signal NN cord-329553-93tbgn2b 230 14 / / SYM cord-329553-93tbgn2b 230 15 anchor anchor NN cord-329553-93tbgn2b 230 16 sequence sequence NN cord-329553-93tbgn2b 230 17 of of IN cord-329553-93tbgn2b 230 18 ST ST NNP cord-329553-93tbgn2b 230 19 is be VBZ cord-329553-93tbgn2b 230 20 within within IN cord-329553-93tbgn2b 230 21 its -PRON- PRP$ cord-329553-93tbgn2b 230 22 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 230 23 33-residue 33-residue CD cord-329553-93tbgn2b 230 24 sequence sequence NN cord-329553-93tbgn2b 230 25 , , , cord-329553-93tbgn2b 230 26 comprising comprise VBG cord-329553-93tbgn2b 230 27 the the DT cord-329553-93tbgn2b 230 28 17-residue 17-residue CD cord-329553-93tbgn2b 230 29 hydrophobic hydrophobic JJ cord-329553-93tbgn2b 230 30 core core NN cord-329553-93tbgn2b 230 31 and and CC cord-329553-93tbgn2b 230 32 flanking flanking JJ cord-329553-93tbgn2b 230 33 hydrophilic hydrophilic JJ cord-329553-93tbgn2b 230 34 sequences sequence NNS cord-329553-93tbgn2b 230 35 on on IN cord-329553-93tbgn2b 230 36 both both DT cord-329553-93tbgn2b 230 37 sides side NNS cord-329553-93tbgn2b 230 38 . . . cord-329553-93tbgn2b 231 1 The the DT cord-329553-93tbgn2b 231 2 nature nature NN cord-329553-93tbgn2b 231 3 of of IN cord-329553-93tbgn2b 231 4 the the DT cord-329553-93tbgn2b 231 5 hydrophilic hydrophilic JJ cord-329553-93tbgn2b 231 6 sequences sequence NNS cord-329553-93tbgn2b 231 7 on on IN cord-329553-93tbgn2b 231 8 either either DT cord-329553-93tbgn2b 231 9 side side NN cord-329553-93tbgn2b 231 10 of of IN cord-329553-93tbgn2b 231 11 the the DT cord-329553-93tbgn2b 231 12 hydrophobic hydrophobic JJ cord-329553-93tbgn2b 231 13 core core NN cord-329553-93tbgn2b 231 14 significantly significantly RB cord-329553-93tbgn2b 231 15 affect affect VBP cord-329553-93tbgn2b 231 16 its -PRON- PRP$ cord-329553-93tbgn2b 231 17 efficiency efficiency NN cord-329553-93tbgn2b 231 18 in in IN cord-329553-93tbgn2b 231 19 acting act VBG cord-329553-93tbgn2b 231 20 as as IN cord-329553-93tbgn2b 231 21 a a DT cord-329553-93tbgn2b 231 22 signal signal NN cord-329553-93tbgn2b 231 23 / / SYM cord-329553-93tbgn2b 231 24 anchor anchor NN cord-329553-93tbgn2b 231 25 sequence sequence NN cord-329553-93tbgn2b 231 26 . . . cord-329553-93tbgn2b 232 1 Results result NNS cord-329553-93tbgn2b 232 2 similar similar JJ cord-329553-93tbgn2b 232 3 to to IN cord-329553-93tbgn2b 232 4 S33D s33d NN cord-329553-93tbgn2b 233 1 ( ( -LRB- cord-329553-93tbgn2b 233 2 Fig Fig NNP cord-329553-93tbgn2b 233 3 . . . cord-329553-93tbgn2b 234 1 3 3 LS cord-329553-93tbgn2b 234 2 ) ) -RRB- cord-329553-93tbgn2b 234 3 were be VBD cord-329553-93tbgn2b 234 4 obtained obtain VBN cord-329553-93tbgn2b 234 5 for for IN cord-329553-93tbgn2b 234 6 S9D s9d NN cord-329553-93tbgn2b 234 7 , , , cord-329553-93tbgn2b 234 8 S10 S10 NNP cord-329553-93tbgn2b 234 9 - - HYPH cord-329553-93tbgn2b 234 10 33D 33d NN cord-329553-93tbgn2b 234 11 , , , cord-329553-93tbgn2b 234 12 and and CC cord-329553-93tbgn2b 234 13 S27 S27 NNP cord-329553-93tbgn2b 234 14 - - HYPH cord-329553-93tbgn2b 234 15 33D 33D NNP cord-329553-93tbgn2b 235 1 ( ( -LRB- cord-329553-93tbgn2b 235 2 data datum NNS cord-329553-93tbgn2b 235 3 not not RB cord-329553-93tbgn2b 235 4 shown show VBN cord-329553-93tbgn2b 235 5 ) ) -RRB- cord-329553-93tbgn2b 235 6 . . . cord-329553-93tbgn2b 236 1 Based base VBN cord-329553-93tbgn2b 236 2 on on IN cord-329553-93tbgn2b 236 3 these these DT cord-329553-93tbgn2b 236 4 in in IN cord-329553-93tbgn2b 236 5 vitro vitro FW cord-329553-93tbgn2b 236 6 translation translation NN cord-329553-93tbgn2b 236 7 results result NNS cord-329553-93tbgn2b 236 8 , , , cord-329553-93tbgn2b 236 9 chimeric chimeric JJ cord-329553-93tbgn2b 236 10 cDNAs cdna NNS cord-329553-93tbgn2b 236 11 encoding encode VBG cord-329553-93tbgn2b 236 12 S9D s9d NN cord-329553-93tbgn2b 236 13 , , , cord-329553-93tbgn2b 236 14 S10 S10 NNP cord-329553-93tbgn2b 236 15 - - HYPH cord-329553-93tbgn2b 236 16 33D 33d NN cord-329553-93tbgn2b 236 17 , , , cord-329553-93tbgn2b 236 18 S10 S10 NNP cord-329553-93tbgn2b 236 19 - - HYPH cord-329553-93tbgn2b 236 20 26D 26d NN cord-329553-93tbgn2b 236 21 , , , cord-329553-93tbgn2b 236 22 and and CC cord-329553-93tbgn2b 236 23 S27 S27 NNP cord-329553-93tbgn2b 236 24 - - HYPH cord-329553-93tbgn2b 236 25 33D 33D NNP cord-329553-93tbgn2b 236 26 ( ( -LRB- cord-329553-93tbgn2b 236 27 S26D s26d NN cord-329553-93tbgn2b 236 28 was be VBD cord-329553-93tbgn2b 236 29 not not RB cord-329553-93tbgn2b 236 30 pursued pursue VBN cord-329553-93tbgn2b 236 31 further further RB cord-329553-93tbgn2b 236 32 ) ) -RRB- cord-329553-93tbgn2b 236 33 were be VBD cord-329553-93tbgn2b 236 34 inserted insert VBN cord-329553-93tbgn2b 236 35 into into IN cord-329553-93tbgn2b 236 36 the the DT cord-329553-93tbgn2b 236 37 pRSN prsn JJ cord-329553-93tbgn2b 236 38 expression expression NN cord-329553-93tbgn2b 236 39 vector vector NN cord-329553-93tbgn2b 236 40 and and CC cord-329553-93tbgn2b 236 41 transfected transfecte VBN cord-329553-93tbgn2b 236 42 into into IN cord-329553-93tbgn2b 236 43 MDCK MDCK NNP cord-329553-93tbgn2b 236 44 cells cell NNS cord-329553-93tbgn2b 236 45 . . . cord-329553-93tbgn2b 237 1 The the DT cord-329553-93tbgn2b 237 2 cellular cellular JJ cord-329553-93tbgn2b 237 3 localization localization NN cord-329553-93tbgn2b 237 4 ofthe ofthe DT cord-329553-93tbgn2b 237 5 fusion fusion NN cord-329553-93tbgn2b 237 6 proteins protein NNS cord-329553-93tbgn2b 237 7 was be VBD cord-329553-93tbgn2b 237 8 examined examine VBN cord-329553-93tbgn2b 237 9 by by IN cord-329553-93tbgn2b 237 10 indirect indirect JJ cord-329553-93tbgn2b 237 11 fluorescence fluorescence NN cord-329553-93tbgn2b 237 12 microscopy microscopy NN cord-329553-93tbgn2b 237 13 . . . cord-329553-93tbgn2b 238 1 As as IN cord-329553-93tbgn2b 238 2 shown show VBN cord-329553-93tbgn2b 238 3 in in IN cord-329553-93tbgn2b 238 4 Fig Fig NNP cord-329553-93tbgn2b 238 5 . . NNP cord-329553-93tbgn2b 238 6 6 6 CD cord-329553-93tbgn2b 238 7 , , , cord-329553-93tbgn2b 238 8 intense intense JJ cord-329553-93tbgn2b 238 9 surface surface NN cord-329553-93tbgn2b 238 10 staining staining NN cord-329553-93tbgn2b 238 11 was be VBD cord-329553-93tbgn2b 238 12 detected detect VBN cord-329553-93tbgn2b 238 13 for for IN cord-329553-93tbgn2b 238 14 S9D s9d NN cord-329553-93tbgn2b 238 15 ( ( -LRB- cord-329553-93tbgn2b 238 16 A a NN cord-329553-93tbgn2b 238 17 ) ) -RRB- cord-329553-93tbgn2b 238 18 and and CC cord-329553-93tbgn2b 238 19 S27 S27 NNP cord-329553-93tbgn2b 238 20 - - HYPH cord-329553-93tbgn2b 238 21 33D 33D NNP cord-329553-93tbgn2b 238 22 ( ( -LRB- cord-329553-93tbgn2b 238 23 C C NNP cord-329553-93tbgn2b 238 24 ) ) -RRB- cord-329553-93tbgn2b 238 25 , , , cord-329553-93tbgn2b 238 26 but but CC cord-329553-93tbgn2b 238 27 not not RB cord-329553-93tbgn2b 238 28 for for IN cord-329553-93tbgn2b 238 29 S10 S10 NNP cord-329553-93tbgn2b 238 30 - - HYPH cord-329553-93tbgn2b 238 31 33D 33d NN cord-329553-93tbgn2b 238 32 ( ( -LRB- cord-329553-93tbgn2b 238 33 E e NN cord-329553-93tbgn2b 238 34 ) ) -RRB- cord-329553-93tbgn2b 238 35 or or CC cord-329553-93tbgn2b 238 36 SIO-26D sio-26d NN cord-329553-93tbgn2b 238 37 ( ( -LRB- cord-329553-93tbgn2b 238 38 G G NNP cord-329553-93tbgn2b 238 39 ) ) -RRB- cord-329553-93tbgn2b 238 40 . . . cord-329553-93tbgn2b 239 1 When when WRB cord-329553-93tbgn2b 239 2 the the DT cord-329553-93tbgn2b 239 3 cells cell NNS cord-329553-93tbgn2b 239 4 were be VBD cord-329553-93tbgn2b 239 5 permeabilized permeabilize VBN cord-329553-93tbgn2b 239 6 , , , cord-329553-93tbgn2b 239 7 intense intense JJ cord-329553-93tbgn2b 239 8 intracellular intracellular JJ cord-329553-93tbgn2b 239 9 staining staining NN cord-329553-93tbgn2b 239 10 ofperinuclear ofperinuclear NN cord-329553-93tbgn2b 239 11 vesicular vesicular JJ cord-329553-93tbgn2b 239 12 - - HYPH cord-329553-93tbgn2b 239 13 tubular tubular JJ cord-329553-93tbgn2b 239 14 network network NN cord-329553-93tbgn2b 239 15 was be VBD cord-329553-93tbgn2b 239 16 observed observe VBN cord-329553-93tbgn2b 239 17 for for IN cord-329553-93tbgn2b 239 18 SIO-33D sio-33d JJ cord-329553-93tbgn2b 239 19 ( ( -LRB- cord-329553-93tbgn2b 239 20 F F NNP cord-329553-93tbgn2b 239 21 ) ) -RRB- cord-329553-93tbgn2b 239 22 and and CC cord-329553-93tbgn2b 239 23 S10 S10 NNP cord-329553-93tbgn2b 239 24 - - HYPH cord-329553-93tbgn2b 239 25 26D 26d NN cord-329553-93tbgn2b 239 26 ( ( -LRB- cord-329553-93tbgn2b 239 27 H H NNP cord-329553-93tbgn2b 239 28 ) ) -RRB- cord-329553-93tbgn2b 239 29 , , , cord-329553-93tbgn2b 239 30 typical typical JJ cord-329553-93tbgn2b 239 31 of of IN cord-329553-93tbgn2b 239 32 the the DT cord-329553-93tbgn2b 239 33 Golgi Golgi NNP cord-329553-93tbgn2b 239 34 apparatus apparatus NN cord-329553-93tbgn2b 239 35 . . . cord-329553-93tbgn2b 240 1 This this DT cord-329553-93tbgn2b 240 2 was be VBD cord-329553-93tbgn2b 240 3 further further RB cord-329553-93tbgn2b 240 4 confirmed confirm VBN cord-329553-93tbgn2b 240 5 by by IN cord-329553-93tbgn2b 240 6 their -PRON- PRP$ cord-329553-93tbgn2b 240 7 colocalization colocalization NN cord-329553-93tbgn2b 240 8 with with IN cord-329553-93tbgn2b 240 9 intracellular intracellular JJ cord-329553-93tbgn2b 240 10 WGA WGA NNP cord-329553-93tbgn2b 240 11 binding bind VBG cord-329553-93tbgn2b 240 12 sites site NNS cord-329553-93tbgn2b 240 13 ( ( -LRB- cord-329553-93tbgn2b 240 14 data datum NNS cord-329553-93tbgn2b 240 15 not not RB cord-329553-93tbgn2b 240 16 shown show VBN cord-329553-93tbgn2b 240 17 ) ) -RRB- cord-329553-93tbgn2b 240 18 . . . cord-329553-93tbgn2b 241 1 S10 S10 NNP cord-329553-93tbgn2b 241 2 - - HYPH cord-329553-93tbgn2b 241 3 26D 26d NN cord-329553-93tbgn2b 241 4 and and CC cord-329553-93tbgn2b 241 5 S10 S10 NNP cord-329553-93tbgn2b 241 6 - - HYPH cord-329553-93tbgn2b 241 7 33D 33d NN cord-329553-93tbgn2b 241 8 have have VBP cord-329553-93tbgn2b 241 9 lectin lectin NN cord-329553-93tbgn2b 241 10 - - HYPH cord-329553-93tbgn2b 241 11 binding bind VBG cord-329553-93tbgn2b 241 12 properties property NNS cord-329553-93tbgn2b 241 13 similar similar JJ cord-329553-93tbgn2b 241 14 to to IN cord-329553-93tbgn2b 241 15 those those DT cord-329553-93tbgn2b 241 16 of of IN cord-329553-93tbgn2b 241 17 S33D s33d NN cord-329553-93tbgn2b 241 18 and and CC cord-329553-93tbgn2b 241 19 S64D S64D NNP cord-329553-93tbgn2b 241 20 , , , cord-329553-93tbgn2b 241 21 demonstrating demonstrate VBG cord-329553-93tbgn2b 241 22 the the DT cord-329553-93tbgn2b 241 23 modification modification NN cord-329553-93tbgn2b 241 24 of of IN cord-329553-93tbgn2b 241 25 their -PRON- PRP$ cord-329553-93tbgn2b 241 26 N n CD cord-329553-93tbgn2b 241 27 - - HYPH cord-329553-93tbgn2b 241 28 linked link VBN cord-329553-93tbgn2b 241 29 glycans glycan NNS cord-329553-93tbgn2b 241 30 by by IN cord-329553-93tbgn2b 241 31 GT GT NNP cord-329553-93tbgn2b 241 32 and and CC cord-329553-93tbgn2b 241 33 sialyltransferases sialyltransferase NNS cord-329553-93tbgn2b 241 34 ( ( -LRB- cord-329553-93tbgn2b 241 35 data datum NNS cord-329553-93tbgn2b 241 36 not not RB cord-329553-93tbgn2b 241 37 shown show VBN cord-329553-93tbgn2b 241 38 ) ) -RRB- cord-329553-93tbgn2b 241 39 . . . cord-329553-93tbgn2b 242 1 S10 S10 NNP cord-329553-93tbgn2b 242 2 - - HYPH cord-329553-93tbgn2b 242 3 26D 26d NN cord-329553-93tbgn2b 242 4 and and CC cord-329553-93tbgn2b 242 5 S10 S10 NNP cord-329553-93tbgn2b 242 6 - - HYPH cord-329553-93tbgn2b 242 7 33D 33d NN cord-329553-93tbgn2b 242 8 thus thus RB cord-329553-93tbgn2b 242 9 reside reside VB cord-329553-93tbgn2b 242 10 in in IN cord-329553-93tbgn2b 242 11 the the DT cord-329553-93tbgn2b 242 12 trans trans NNP cord-329553-93tbgn2b 242 13 - - NNP cord-329553-93tbgn2b 242 14 Golgi Golgi NNP cord-329553-93tbgn2b 242 15 cisternae cisternae NNP cord-329553-93tbgn2b 242 16 and/or and/or CC cord-329553-93tbgn2b 242 17 TGN TGN NNP cord-329553-93tbgn2b 242 18 . . . cord-329553-93tbgn2b 243 1 To to TO cord-329553-93tbgn2b 243 2 obtain obtain VB cord-329553-93tbgn2b 243 3 quantitative quantitative JJ cord-329553-93tbgn2b 243 4 data datum NNS cord-329553-93tbgn2b 243 5 on on IN cord-329553-93tbgn2b 243 6 the the DT cord-329553-93tbgn2b 243 7 intracellular intracellular JJ cord-329553-93tbgn2b 243 8 Golgi Golgi NNP cord-329553-93tbgn2b 243 9 localization localization NN cord-329553-93tbgn2b 243 10 of of IN cord-329553-93tbgn2b 243 11 the the DT cord-329553-93tbgn2b 243 12 various various JJ cord-329553-93tbgn2b 243 13 fusion fusion NN cord-329553-93tbgn2b 243 14 proteins protein NNS cord-329553-93tbgn2b 243 15 , , , cord-329553-93tbgn2b 243 16 we -PRON- PRP cord-329553-93tbgn2b 243 17 used use VBD cord-329553-93tbgn2b 243 18 a a DT cord-329553-93tbgn2b 243 19 biochemical biochemical JJ cord-329553-93tbgn2b 243 20 approach approach NN cord-329553-93tbgn2b 243 21 to to TO cord-329553-93tbgn2b 243 22 determine determine VB cord-329553-93tbgn2b 243 23 the the DT cord-329553-93tbgn2b 243 24 levels level NNS cord-329553-93tbgn2b 243 25 of of IN cord-329553-93tbgn2b 243 26 surface surface NN cord-329553-93tbgn2b 243 27 expression expression NN cord-329553-93tbgn2b 243 28 of of IN cord-329553-93tbgn2b 243 29 respective respective JJ cord-329553-93tbgn2b 243 30 proteins protein NNS cord-329553-93tbgn2b 243 31 ( ( -LRB- cord-329553-93tbgn2b 243 32 Fig Fig NNP cord-329553-93tbgn2b 243 33 . . NNP cord-329553-93tbgn2b 243 34 7 7 CD cord-329553-93tbgn2b 243 35 ) ) -RRB- cord-329553-93tbgn2b 244 1 ( ( -LRB- cord-329553-93tbgn2b 244 2 Bretscher Bretscher NNP cord-329553-93tbgn2b 244 3 and and CC cord-329553-93tbgn2b 244 4 Lutter Lutter NNP cord-329553-93tbgn2b 244 5 , , , cord-329553-93tbgn2b 244 6 1988 1988 CD cord-329553-93tbgn2b 244 7 ; ; : cord-329553-93tbgn2b 244 8 Le Le NNP cord-329553-93tbgn2b 244 9 Bivic Bivic NNP cord-329553-93tbgn2b 244 10 et et FW cord-329553-93tbgn2b 244 11 al al NNP cord-329553-93tbgn2b 244 12 . . NNP cord-329553-93tbgn2b 244 13 , , , cord-329553-93tbgn2b 244 14 1990a 1990a CD cord-329553-93tbgn2b 244 15 , , , cord-329553-93tbgn2b 244 16 b b LS cord-329553-93tbgn2b 244 17 ; ; : cord-329553-93tbgn2b 244 18 Low Low NNP cord-329553-93tbgn2b 244 19 et et FW cord-329553-93tbgn2b 244 20 al al NNP cord-329553-93tbgn2b 244 21 . . NNP cord-329553-93tbgn2b 244 22 , , , cord-329553-93tbgn2b 244 23 199la 199la NNP cord-329553-93tbgn2b 244 24 , , , cord-329553-93tbgn2b 244 25 b b NN cord-329553-93tbgn2b 244 26 ) ) -RRB- cord-329553-93tbgn2b 244 27 . . . cord-329553-93tbgn2b 245 1 For for IN cord-329553-93tbgn2b 245 2 convenience convenience NN cord-329553-93tbgn2b 245 3 , , , cord-329553-93tbgn2b 245 4 we -PRON- PRP cord-329553-93tbgn2b 245 5 arbitrarily arbitrarily RB cord-329553-93tbgn2b 245 6 defined define VBD cord-329553-93tbgn2b 245 7 the the DT cord-329553-93tbgn2b 245 8 surface surface NN cord-329553-93tbgn2b 245 9 expression expression NN cord-329553-93tbgn2b 245 10 of of IN cord-329553-93tbgn2b 245 11 DPPIV DPPIV NNP cord-329553-93tbgn2b 245 12 as as IN cord-329553-93tbgn2b 245 13 100 100 CD cord-329553-93tbgn2b 245 14 % % NN cord-329553-93tbgn2b 245 15 . . . cord-329553-93tbgn2b 246 1 The the DT cord-329553-93tbgn2b 246 2 extent extent NN cord-329553-93tbgn2b 246 3 of of IN cord-329553-93tbgn2b 246 4 surface surface NN cord-329553-93tbgn2b 246 5 expression expression NN cord-329553-93tbgn2b 246 6 for for IN cord-329553-93tbgn2b 246 7 other other JJ cord-329553-93tbgn2b 246 8 proteins protein NNS cord-329553-93tbgn2b 246 9 was be VBD cord-329553-93tbgn2b 246 10 normalized normalize VBN cord-329553-93tbgn2b 246 11 with with IN cord-329553-93tbgn2b 246 12 that that DT cord-329553-93tbgn2b 246 13 ofDPPIV ofDPPIV NNP cord-329553-93tbgn2b 246 14 and and CC cord-329553-93tbgn2b 246 15 defined define VBN cord-329553-93tbgn2b 246 16 as as IN cord-329553-93tbgn2b 246 17 a a DT cord-329553-93tbgn2b 246 18 percentage percentage NN cord-329553-93tbgn2b 246 19 of of IN cord-329553-93tbgn2b 246 20 surface surface NN cord-329553-93tbgn2b 246 21 expression expression NN cord-329553-93tbgn2b 246 22 . . . cord-329553-93tbgn2b 247 1 As as IN cord-329553-93tbgn2b 247 2 shown show VBN cord-329553-93tbgn2b 247 3 , , , cord-329553-93tbgn2b 247 4 the the DT cord-329553-93tbgn2b 247 5 surface surface NN cord-329553-93tbgn2b 247 6 expression expression NN cord-329553-93tbgn2b 247 7 of of IN cord-329553-93tbgn2b 247 8 S33D s33d NN cord-329553-93tbgn2b 247 9 , , , cord-329553-93tbgn2b 247 10 S64D s64d NN cord-329553-93tbgn2b 247 11 , , , cord-329553-93tbgn2b 247 12 S10 S10 NNP cord-329553-93tbgn2b 247 13 - - HYPH cord-329553-93tbgn2b 247 14 33D 33d NN cord-329553-93tbgn2b 247 15 , , , cord-329553-93tbgn2b 247 16 and and CC cord-329553-93tbgn2b 247 17 S10 S10 NNP cord-329553-93tbgn2b 247 18 - - HYPH cord-329553-93tbgn2b 247 19 26D 26D NNP cord-329553-93tbgn2b 247 20 was be VBD cord-329553-93tbgn2b 247 21 between between IN cord-329553-93tbgn2b 247 22 2 2 CD cord-329553-93tbgn2b 247 23 and and CC cord-329553-93tbgn2b 247 24 6 6 CD cord-329553-93tbgn2b 247 25 % % NN cord-329553-93tbgn2b 247 26 as as IN cord-329553-93tbgn2b 247 27 compared compare VBN cord-329553-93tbgn2b 247 28 with with IN cord-329553-93tbgn2b 247 29 DPPIV DPPIV NNP cord-329553-93tbgn2b 247 30 . . . cord-329553-93tbgn2b 248 1 The the DT cord-329553-93tbgn2b 248 2 loss loss NN cord-329553-93tbgn2b 248 3 of of IN cord-329553-93tbgn2b 248 4 surface surface NN cord-329553-93tbgn2b 248 5 expression expression NN cord-329553-93tbgn2b 248 6 demonstrates demonstrate VBZ cord-329553-93tbgn2b 248 7 that that IN cord-329553-93tbgn2b 248 8 the the DT cord-329553-93tbgn2b 248 9 Golgi Golgi NNP cord-329553-93tbgn2b 248 10 localization localization NN cord-329553-93tbgn2b 248 11 signal signal NN cord-329553-93tbgn2b 248 12 of of IN cord-329553-93tbgn2b 248 13 ST ST NNP cord-329553-93tbgn2b 248 14 is be VBZ cord-329553-93tbgn2b 248 15 highly highly RB cord-329553-93tbgn2b 248 16 effective effective JJ cord-329553-93tbgn2b 248 17 in in IN cord-329553-93tbgn2b 248 18 targeting target VBG cord-329553-93tbgn2b 248 19 the the DT cord-329553-93tbgn2b 248 20 fusion fusion NN cord-329553-93tbgn2b 248 21 proteins protein NNS cord-329553-93tbgn2b 248 22 to to IN cord-329553-93tbgn2b 248 23 the the DT cord-329553-93tbgn2b 248 24 Golgi Golgi NNP cord-329553-93tbgn2b 248 25 apparatus apparatus NN cord-329553-93tbgn2b 248 26 . . . cord-329553-93tbgn2b 249 1 In in IN cord-329553-93tbgn2b 249 2 contrast contrast NN cord-329553-93tbgn2b 249 3 , , , cord-329553-93tbgn2b 249 4 high high JJ cord-329553-93tbgn2b 249 5 levels level NNS cord-329553-93tbgn2b 249 6 of of IN cord-329553-93tbgn2b 249 7 surface surface NN cord-329553-93tbgn2b 249 8 expression expression NN cord-329553-93tbgn2b 249 9 , , , cord-329553-93tbgn2b 249 10 comparable comparable JJ cord-329553-93tbgn2b 249 11 to to IN cord-329553-93tbgn2b 249 12 DPPIV DPPIV NNP cord-329553-93tbgn2b 249 13 , , , cord-329553-93tbgn2b 249 14 were be VBD cord-329553-93tbgn2b 249 15 observed observe VBN cord-329553-93tbgn2b 249 16 for for IN cord-329553-93tbgn2b 249 17 S9D s9d NN cord-329553-93tbgn2b 249 18 and and CC cord-329553-93tbgn2b 249 19 S27- s27- NN cord-329553-93tbgn2b 250 1 33D. 33d. LS cord-329553-93tbgn2b 251 1 These these DT cord-329553-93tbgn2b 251 2 biochemical biochemical JJ cord-329553-93tbgn2b 251 3 results result NNS cord-329553-93tbgn2b 251 4 are be VBP cord-329553-93tbgn2b 251 5 consistent consistent JJ cord-329553-93tbgn2b 251 6 with with IN cord-329553-93tbgn2b 251 7 and and CC cord-329553-93tbgn2b 251 8 further further RB cord-329553-93tbgn2b 251 9 extend extend VB cord-329553-93tbgn2b 251 10 the the DT cord-329553-93tbgn2b 251 11 indirect indirect JJ cord-329553-93tbgn2b 251 12 immunofluorescence immunofluorescence NN cord-329553-93tbgn2b 251 13 data datum NNS cord-329553-93tbgn2b 251 14 . . . cord-329553-93tbgn2b 252 1 Newly newly RB cord-329553-93tbgn2b 252 2 made make VBN cord-329553-93tbgn2b 252 3 molecules molecule NNS cord-329553-93tbgn2b 252 4 of of IN cord-329553-93tbgn2b 252 5 S33D s33d NN cord-329553-93tbgn2b 252 6 , , , cord-329553-93tbgn2b 252 7 S64D s64d NN cord-329553-93tbgn2b 252 8 , , , cord-329553-93tbgn2b 252 9 S10 S10 NNP cord-329553-93tbgn2b 252 10 - - HYPH cord-329553-93tbgn2b 252 11 33D 33d NN cord-329553-93tbgn2b 252 12 , , , cord-329553-93tbgn2b 252 13 and and CC cord-329553-93tbgn2b 252 14 S10 S10 NNP cord-329553-93tbgn2b 252 15 - - HYPH cord-329553-93tbgn2b 252 16 26D 26d NN cord-329553-93tbgn2b 252 17 were be VBD cord-329553-93tbgn2b 252 18 hardly hardly RB cord-329553-93tbgn2b 252 19 detectable detectable JJ cord-329553-93tbgn2b 252 20 on on IN cord-329553-93tbgn2b 252 21 the the DT cord-329553-93tbgn2b 252 22 cell cell NN cord-329553-93tbgn2b 252 23 surface surface NN cord-329553-93tbgn2b 252 24 after after IN cord-329553-93tbgn2b 252 25 3 3 CD cord-329553-93tbgn2b 252 26 h h NN cord-329553-93tbgn2b 252 27 of of IN cord-329553-93tbgn2b 252 28 chase chase NNP cord-329553-93tbgn2b 252 29 ( ( -LRB- cord-329553-93tbgn2b 252 30 Fig Fig NNP cord-329553-93tbgn2b 252 31 . . NNP cord-329553-93tbgn2b 252 32 7 7 CD cord-329553-93tbgn2b 252 33 ) ) -RRB- cord-329553-93tbgn2b 252 34 . . . cord-329553-93tbgn2b 253 1 In in IN cord-329553-93tbgn2b 253 2 connection connection NN cord-329553-93tbgn2b 253 3 with with IN cord-329553-93tbgn2b 253 4 the the DT cord-329553-93tbgn2b 253 5 predominant predominant JJ cord-329553-93tbgn2b 253 6 Golgi Golgi NNP cord-329553-93tbgn2b 253 7 staining staining NN cord-329553-93tbgn2b 253 8 of of IN cord-329553-93tbgn2b 253 9 these these DT cord-329553-93tbgn2b 253 10 proteins protein NNS cord-329553-93tbgn2b 253 11 , , , cord-329553-93tbgn2b 253 12 these these DT cord-329553-93tbgn2b 253 13 results result NNS cord-329553-93tbgn2b 253 14 suggest suggest VBP cord-329553-93tbgn2b 253 15 that that IN cord-329553-93tbgn2b 253 16 they -PRON- PRP cord-329553-93tbgn2b 253 17 are be VBP cord-329553-93tbgn2b 253 18 specifically specifically RB cord-329553-93tbgn2b 253 19 and and CC cord-329553-93tbgn2b 253 20 efficiently efficiently RB cord-329553-93tbgn2b 253 21 retained retain VBN cord-329553-93tbgn2b 253 22 in in IN cord-329553-93tbgn2b 253 23 the the DT cord-329553-93tbgn2b 253 24 Golgi Golgi NNP cord-329553-93tbgn2b 253 25 apparatus apparatus NN cord-329553-93tbgn2b 253 26 . . . cord-329553-93tbgn2b 254 1 However however RB cord-329553-93tbgn2b 254 2 , , , cord-329553-93tbgn2b 254 3 the the DT cord-329553-93tbgn2b 254 4 possibility possibility NN cord-329553-93tbgn2b 254 5 that that IN cord-329553-93tbgn2b 254 6 they -PRON- PRP cord-329553-93tbgn2b 254 7 may may MD cord-329553-93tbgn2b 254 8 be be VB cord-329553-93tbgn2b 254 9 initially initially RB cord-329553-93tbgn2b 254 10 transported transport VBN cord-329553-93tbgn2b 254 11 to to IN cord-329553-93tbgn2b 254 12 the the DT cord-329553-93tbgn2b 254 13 cell cell NN cord-329553-93tbgn2b 254 14 surface surface NN cord-329553-93tbgn2b 254 15 and and CC cord-329553-93tbgn2b 254 16 then then RB cord-329553-93tbgn2b 254 17 rapidly rapidly RB cord-329553-93tbgn2b 254 18 and and CC cord-329553-93tbgn2b 254 19 effectively effectively RB cord-329553-93tbgn2b 254 20 endocytosed endocytose VBD cord-329553-93tbgn2b 254 21 and and CC cord-329553-93tbgn2b 254 22 sorted sort VBN cord-329553-93tbgn2b 254 23 to to IN cord-329553-93tbgn2b 254 24 the the DT cord-329553-93tbgn2b 254 25 trans trans NNP cord-329553-93tbgn2b 254 26 - - NNP cord-329553-93tbgn2b 254 27 Golgi Golgi NNP cord-329553-93tbgn2b 254 28 cisternae cisternae NN cord-329553-93tbgn2b 255 1 and/or and/or CC cord-329553-93tbgn2b 255 2 TGN TGN NNP cord-329553-93tbgn2b 255 3 could could MD cord-329553-93tbgn2b 255 4 not not RB cord-329553-93tbgn2b 255 5 be be VB cord-329553-93tbgn2b 255 6 ruled rule VBN cord-329553-93tbgn2b 255 7 out out RP cord-329553-93tbgn2b 255 8 because because IN cord-329553-93tbgn2b 255 9 only only RB cord-329553-93tbgn2b 255 10 a a DT cord-329553-93tbgn2b 255 11 single single JJ cord-329553-93tbgn2b 255 12 chase chase NN cord-329553-93tbgn2b 255 13 point point NN cord-329553-93tbgn2b 255 14 was be VBD cord-329553-93tbgn2b 255 15 examined examine VBN cord-329553-93tbgn2b 255 16 . . . cord-329553-93tbgn2b 256 1 If if IN cord-329553-93tbgn2b 256 2 endocytosis endocytosis NN cord-329553-93tbgn2b 256 3 followed follow VBN cord-329553-93tbgn2b 256 4 by by IN cord-329553-93tbgn2b 256 5 sorting sort VBG cord-329553-93tbgn2b 256 6 is be VBZ cord-329553-93tbgn2b 256 7 the the DT cord-329553-93tbgn2b 256 8 mechanism mechanism NN cord-329553-93tbgn2b 256 9 for for IN cord-329553-93tbgn2b 256 10 their -PRON- PRP$ cord-329553-93tbgn2b 256 11 Golgi Golgi NNP cord-329553-93tbgn2b 256 12 localization localization NN cord-329553-93tbgn2b 256 13 , , , cord-329553-93tbgn2b 256 14 a a DT cord-329553-93tbgn2b 256 15 peak peak NN cord-329553-93tbgn2b 256 16 in in IN cord-329553-93tbgn2b 256 17 surface surface NN cord-329553-93tbgn2b 256 18 expression expression NN cord-329553-93tbgn2b 256 19 should should MD cord-329553-93tbgn2b 256 20 be be VB cord-329553-93tbgn2b 256 21 expected expect VBN cord-329553-93tbgn2b 256 22 during during IN cord-329553-93tbgn2b 256 23 a a DT cord-329553-93tbgn2b 256 24 series series NN cord-329553-93tbgn2b 256 25 of of IN cord-329553-93tbgn2b 256 26 chase chase NN cord-329553-93tbgn2b 256 27 intervals interval NNS cord-329553-93tbgn2b 256 28 after after IN cord-329553-93tbgn2b 256 29 a a DT cord-329553-93tbgn2b 256 30 single single JJ cord-329553-93tbgn2b 256 31 pulse pulse NN cord-329553-93tbgn2b 256 32 - - HYPH cord-329553-93tbgn2b 256 33 labeling labeling NN cord-329553-93tbgn2b 256 34 . . . cord-329553-93tbgn2b 257 1 Cells cell NNS cord-329553-93tbgn2b 257 2 expressing express VBG cord-329553-93tbgn2b 257 3 S33D s33d RB cord-329553-93tbgn2b 257 4 , , , cord-329553-93tbgn2b 257 5 S10 S10 NNP cord-329553-93tbgn2b 257 6 - - HYPH cord-329553-93tbgn2b 257 7 26D 26d NN cord-329553-93tbgn2b 257 8 , , , cord-329553-93tbgn2b 257 9 and and CC cord-329553-93tbgn2b 257 10 DPPIV DPPIV NNP cord-329553-93tbgn2b 257 11 were be VBD cord-329553-93tbgn2b 257 12 selected select VBN cord-329553-93tbgn2b 257 13 for for IN cord-329553-93tbgn2b 257 14 further further JJ cord-329553-93tbgn2b 257 15 examination examination NN cord-329553-93tbgn2b 257 16 . . . cord-329553-93tbgn2b 258 1 Cells cell NNS cord-329553-93tbgn2b 258 2 were be VBD cord-329553-93tbgn2b 258 3 pulse pulse NN cord-329553-93tbgn2b 258 4 - - HYPH cord-329553-93tbgn2b 258 5 labeled label VBN cord-329553-93tbgn2b 258 6 with with IN cord-329553-93tbgn2b 258 7 [ [ -LRB- cord-329553-93tbgn2b 258 8 ' ' '' cord-329553-93tbgn2b 258 9 SS]methionine SS]methionine NNP cord-329553-93tbgn2b 258 10 for for IN cord-329553-93tbgn2b 258 11 30 30 CD cord-329553-93tbgn2b 258 12 min min NN cord-329553-93tbgn2b 258 13 . . . cord-329553-93tbgn2b 259 1 After after IN cord-329553-93tbgn2b 259 2 chasing chase VBG cord-329553-93tbgn2b 259 3 for for IN cord-329553-93tbgn2b 259 4 15 15 CD cord-329553-93tbgn2b 259 5 min min NN cord-329553-93tbgn2b 259 6 , , , cord-329553-93tbgn2b 259 7 45 45 CD cord-329553-93tbgn2b 259 8 min min NN cord-329553-93tbgn2b 259 9 , , , cord-329553-93tbgn2b 259 10 90 90 CD cord-329553-93tbgn2b 259 11 min min NN cord-329553-93tbgn2b 259 12 , , , cord-329553-93tbgn2b 259 13 2 2 CD cord-329553-93tbgn2b 259 14 h h NN cord-329553-93tbgn2b 259 15 , , , cord-329553-93tbgn2b 259 16 6 6 CD cord-329553-93tbgn2b 259 17 h h NN cord-329553-93tbgn2b 259 18 , , , cord-329553-93tbgn2b 259 19 and and CC cord-329553-93tbgn2b 259 20 22 22 CD cord-329553-93tbgn2b 259 21 h h NN cord-329553-93tbgn2b 259 22 , , , cord-329553-93tbgn2b 259 23 cells cell NNS cord-329553-93tbgn2b 259 24 were be VBD cord-329553-93tbgn2b 259 25 surface surface NN cord-329553-93tbgn2b 259 26 biotinylated biotinylate VBN cord-329553-93tbgn2b 259 27 with with IN cord-329553-93tbgn2b 259 28 NHSbiotin NHSbiotin NNS cord-329553-93tbgn2b 259 29 and and CC cord-329553-93tbgn2b 259 30 cell cell NN cord-329553-93tbgn2b 259 31 lysates lysate NNS cord-329553-93tbgn2b 259 32 were be VBD cord-329553-93tbgn2b 259 33 immunoprecipitated immunoprecipitate VBN cord-329553-93tbgn2b 259 34 . . . cord-329553-93tbgn2b 260 1 One one CD cord-329553-93tbgn2b 260 2 tenth tenth NN cord-329553-93tbgn2b 260 3 of of IN cord-329553-93tbgn2b 260 4 the the DT cord-329553-93tbgn2b 260 5 immunoprecipitate immunoprecipitate NN cord-329553-93tbgn2b 260 6 served serve VBD cord-329553-93tbgn2b 260 7 as as IN cord-329553-93tbgn2b 260 8 a a DT cord-329553-93tbgn2b 260 9 measure measure NN cord-329553-93tbgn2b 260 10 of of IN cord-329553-93tbgn2b 260 11 the the DT cord-329553-93tbgn2b 260 12 total total JJ cord-329553-93tbgn2b 260 13 amount amount NN cord-329553-93tbgn2b 260 14 of of IN cord-329553-93tbgn2b 260 15 labeled label VBN cord-329553-93tbgn2b 260 16 fusion fusion NN cord-329553-93tbgn2b 260 17 protein protein NN cord-329553-93tbgn2b 260 18 ( ( -LRB- cord-329553-93tbgn2b 260 19 1/10 1/10 CD cord-329553-93tbgn2b 260 20 Total Total NNP cord-329553-93tbgn2b 260 21 ) ) -RRB- cord-329553-93tbgn2b 260 22 , , , cord-329553-93tbgn2b 260 23 while while IN cord-329553-93tbgn2b 260 24 nine nine CD cord-329553-93tbgn2b 260 25 tenths tenth NNS cord-329553-93tbgn2b 260 26 was be VBD cord-329553-93tbgn2b 260 27 absorbed absorb VBN cord-329553-93tbgn2b 260 28 with with IN cord-329553-93tbgn2b 260 29 streptavidin streptavidin NNS cord-329553-93tbgn2b 260 30 - - HYPH cord-329553-93tbgn2b 260 31 agarose agarose VBN cord-329553-93tbgn2b 260 32 to to TO cord-329553-93tbgn2b 260 33 recover recover VB cord-329553-93tbgn2b 260 34 the the DT cord-329553-93tbgn2b 260 35 biotinylated biotinylate VBN cord-329553-93tbgn2b 260 36 surface surface NN cord-329553-93tbgn2b 260 37 proteins protein NNS cord-329553-93tbgn2b 260 38 ( ( -LRB- cord-329553-93tbgn2b 260 39 Surface surface NN cord-329553-93tbgn2b 260 40 ) ) -RRB- cord-329553-93tbgn2b 261 1 ( ( -LRB- cord-329553-93tbgn2b 261 2 Fig Fig NNP cord-329553-93tbgn2b 261 3 . . . cord-329553-93tbgn2b 261 4 8) 8) NNP cord-329553-93tbgn2b 261 5 . . . cord-329553-93tbgn2b 262 1 All all DT cord-329553-93tbgn2b 262 2 were be VBD cord-329553-93tbgn2b 262 3 initially initially RB cord-329553-93tbgn2b 262 4 made make VBN cord-329553-93tbgn2b 262 5 as as IN cord-329553-93tbgn2b 262 6 the the DT cord-329553-93tbgn2b 262 7 100-kD 100-kd CD cord-329553-93tbgn2b 262 8 form form NN cord-329553-93tbgn2b 262 9 followed follow VBN cord-329553-93tbgn2b 262 10 by by IN cord-329553-93tbgn2b 262 11 rapid rapid JJ cord-329553-93tbgn2b 262 12 conversion conversion NN cord-329553-93tbgn2b 262 13 into into IN cord-329553-93tbgn2b 262 14 the the DT cord-329553-93tbgn2b 262 15 110-kD 110-kd CD cord-329553-93tbgn2b 262 16 form form NN cord-329553-93tbgn2b 262 17 , , , cord-329553-93tbgn2b 262 18 demonstrating demonstrate VBG cord-329553-93tbgn2b 262 19 that that IN cord-329553-93tbgn2b 262 20 they -PRON- PRP cord-329553-93tbgn2b 262 21 were be VBD cord-329553-93tbgn2b 262 22 efficiently efficiently RB cord-329553-93tbgn2b 262 23 transported transport VBN cord-329553-93tbgn2b 262 24 from from IN cord-329553-93tbgn2b 262 25 the the DT cord-329553-93tbgn2b 262 26 ER ER NNP cord-329553-93tbgn2b 262 27 to to IN cord-329553-93tbgn2b 262 28 the the DT cord-329553-93tbgn2b 262 29 Golgi Golgi NNP cord-329553-93tbgn2b 262 30 apparatus apparatus NN cord-329553-93tbgn2b 262 31 . . . cord-329553-93tbgn2b 263 1 Strong strong JJ cord-329553-93tbgn2b 263 2 surface surface NN cord-329553-93tbgn2b 263 3 expression expression NN cord-329553-93tbgn2b 263 4 was be VBD cord-329553-93tbgn2b 263 5 detected detect VBN cord-329553-93tbgn2b 263 6 for for IN cord-329553-93tbgn2b 263 7 DPPIV DPPIV NNP cord-329553-93tbgn2b 263 8 , , , cord-329553-93tbgn2b 263 9 and and CC cord-329553-93tbgn2b 263 10 an an DT cord-329553-93tbgn2b 263 11 almost almost RB cord-329553-93tbgn2b 263 12 maximal maximal JJ cord-329553-93tbgn2b 263 13 level level NN cord-329553-93tbgn2b 263 14 of of IN cord-329553-93tbgn2b 263 15 surface surface NN cord-329553-93tbgn2b 263 16 expression expression NN cord-329553-93tbgn2b 263 17 was be VBD cord-329553-93tbgn2b 263 18 detected detect VBN cord-329553-93tbgn2b 263 19 after after IN cord-329553-93tbgn2b 263 20 90 90 CD cord-329553-93tbgn2b 263 21 min min NN cord-329553-93tbgn2b 263 22 of of IN cord-329553-93tbgn2b 263 23 chase chase NN cord-329553-93tbgn2b 263 24 . . . cord-329553-93tbgn2b 264 1 Since since IN cord-329553-93tbgn2b 264 2 S33D s33d JJ cord-329553-93tbgn2b 264 3 and and CC cord-329553-93tbgn2b 264 4 S10 S10 NNP cord-329553-93tbgn2b 264 5 - - HYPH cord-329553-93tbgn2b 264 6 26D 26d NN cord-329553-93tbgn2b 264 7 were be VBD cord-329553-93tbgn2b 264 8 transported transport VBN cord-329553-93tbgn2b 264 9 to to IN cord-329553-93tbgn2b 264 10 the the DT cord-329553-93tbgn2b 264 11 Golgi Golgi NNP cord-329553-93tbgn2b 264 12 apparatus apparatus NN cord-329553-93tbgn2b 264 13 as as RB cord-329553-93tbgn2b 264 14 efficiently efficiently RB cord-329553-93tbgn2b 264 15 as as IN cord-329553-93tbgn2b 264 16 DPPIV DPPIV NNP cord-329553-93tbgn2b 264 17 , , , cord-329553-93tbgn2b 264 18 a a DT cord-329553-93tbgn2b 264 19 peak peak NN cord-329553-93tbgn2b 264 20 expression expression NN cord-329553-93tbgn2b 264 21 on on IN cord-329553-93tbgn2b 264 22 the the DT cord-329553-93tbgn2b 264 23 surface surface NN cord-329553-93tbgn2b 264 24 at at IN cord-329553-93tbgn2b 264 25 -90 -90 NN cord-329553-93tbgn2b 264 26 min min NNP cord-329553-93tbgn2b 264 27 of of IN cord-329553-93tbgn2b 264 28 chase chase NN cord-329553-93tbgn2b 264 29 should should MD cord-329553-93tbgn2b 264 30 be be VB cord-329553-93tbgn2b 264 31 ex- ex- VBN cord-329553-93tbgn2b 264 32 ] ] -RRB- cord-329553-93tbgn2b 265 1 methionine methionine NNP cord-329553-93tbgn2b 265 2 for for IN cord-329553-93tbgn2b 265 3 30 30 CD cord-329553-93tbgn2b 265 4 min min NN cord-329553-93tbgn2b 265 5 followed follow VBN cord-329553-93tbgn2b 265 6 by by IN cord-329553-93tbgn2b 265 7 3 3 CD cord-329553-93tbgn2b 265 8 h h NNP cord-329553-93tbgn2b 265 9 of of IN cord-329553-93tbgn2b 265 10 chase chase NNP cord-329553-93tbgn2b 265 11 . . . cord-329553-93tbgn2b 266 1 Proteins protein NNS cord-329553-93tbgn2b 266 2 exposed expose VBN cord-329553-93tbgn2b 266 3 on on IN cord-329553-93tbgn2b 266 4 the the DT cord-329553-93tbgn2b 266 5 cell cell NN cord-329553-93tbgn2b 266 6 surface surface NN cord-329553-93tbgn2b 266 7 were be VBD cord-329553-93tbgn2b 266 8 biotinylated biotinylate VBN cord-329553-93tbgn2b 266 9 with with IN cord-329553-93tbgn2b 266 10 NHS NHS NNP cord-329553-93tbgn2b 266 11 - - HYPH cord-329553-93tbgn2b 266 12 biotin biotin NN cord-329553-93tbgn2b 266 13 . . . cord-329553-93tbgn2b 267 1 Cell cell NN cord-329553-93tbgn2b 267 2 lysates lysate NNS cord-329553-93tbgn2b 267 3 were be VBD cord-329553-93tbgn2b 267 4 immunoprecipitated immunoprecipitate VBN cord-329553-93tbgn2b 267 5 with with IN cord-329553-93tbgn2b 267 6 monoclonal monoclonal JJ cord-329553-93tbgn2b 267 7 antibodies antibody NNS cord-329553-93tbgn2b 267 8 against against IN cord-329553-93tbgn2b 267 9 DPPIV DPPIV NNP cord-329553-93tbgn2b 267 10 . . . cord-329553-93tbgn2b 268 1 One one CD cord-329553-93tbgn2b 268 2 tenth tenth NN cord-329553-93tbgn2b 268 3 of of IN cord-329553-93tbgn2b 268 4 the the DT cord-329553-93tbgn2b 268 5 immunocomplex immunocomplex NN cord-329553-93tbgn2b 268 6 was be VBD cord-329553-93tbgn2b 268 7 analyzed analyze VBN cord-329553-93tbgn2b 268 8 directly directly RB cord-329553-93tbgn2b 268 9 and and CC cord-329553-93tbgn2b 268 10 served serve VBD cord-329553-93tbgn2b 268 11 as as IN cord-329553-93tbgn2b 268 12 a a DT cord-329553-93tbgn2b 268 13 measure measure NN cord-329553-93tbgn2b 268 14 ofthetotal ofthetotal JJ cord-329553-93tbgn2b 268 15 amount amount NN cord-329553-93tbgn2b 268 16 of of IN cord-329553-93tbgn2b 268 17 the the DT cord-329553-93tbgn2b 268 18 respective respective JJ cord-329553-93tbgn2b 268 19 protein protein NN cord-329553-93tbgn2b 268 20 ( ( -LRB- cord-329553-93tbgn2b 268 21 1/107 1/107 CD cord-329553-93tbgn2b 268 22 ) ) -RRB- cord-329553-93tbgn2b 268 23 . . . cord-329553-93tbgn2b 269 1 The the DT cord-329553-93tbgn2b 269 2 remainingnine remainingnine NN cord-329553-93tbgn2b 269 3 tenths tenth NNS cord-329553-93tbgn2b 269 4 was be VBD cord-329553-93tbgn2b 269 5 absorbed absorb VBN cord-329553-93tbgn2b 269 6 to to IN cord-329553-93tbgn2b 269 7 streptavidin streptavidin NN cord-329553-93tbgn2b 269 8 - - HYPH cord-329553-93tbgn2b 269 9 agarose agarose VBN cord-329553-93tbgn2b 269 10 to to TO cord-329553-93tbgn2b 269 11 recover recover VB cord-329553-93tbgn2b 269 12 the the DT cord-329553-93tbgn2b 269 13 biotinylated biotinylated JJ cord-329553-93tbgn2b 269 14 proteins protein NNS cord-329553-93tbgn2b 269 15 and and CC cord-329553-93tbgn2b 269 16 analyzed analyze VBN cord-329553-93tbgn2b 269 17 ( ( -LRB- cord-329553-93tbgn2b 269 18 Surf surf NN cord-329553-93tbgn2b 269 19 ) ) -RRB- cord-329553-93tbgn2b 269 20 . . . cord-329553-93tbgn2b 270 1 This this DT cord-329553-93tbgn2b 270 2 served serve VBD cord-329553-93tbgn2b 270 3 as as IN cord-329553-93tbgn2b 270 4 a a DT cord-329553-93tbgn2b 270 5 measure measure NN cord-329553-93tbgn2b 270 6 of of IN cord-329553-93tbgn2b 270 7 the the DT cord-329553-93tbgn2b 270 8 amount amount NN cord-329553-93tbgn2b 270 9 that that WDT cord-329553-93tbgn2b 270 10 was be VBD cord-329553-93tbgn2b 270 11 expressed express VBN cord-329553-93tbgn2b 270 12 on on IN cord-329553-93tbgn2b 270 13 the the DT cord-329553-93tbgn2b 270 14 cell cell NN cord-329553-93tbgn2b 270 15 surface surface NN cord-329553-93tbgn2b 270 16 . . . cord-329553-93tbgn2b 271 1 ( ( -LRB- cord-329553-93tbgn2b 271 2 B b NN cord-329553-93tbgn2b 271 3 ) ) -RRB- cord-329553-93tbgn2b 271 4 Quantitation quantitation NN cord-329553-93tbgn2b 271 5 of of IN cord-329553-93tbgn2b 271 6 the the DT cord-329553-93tbgn2b 271 7 results result NNS cord-329553-93tbgn2b 271 8 in in IN cord-329553-93tbgn2b 271 9 A. a. NN cord-329553-93tbgn2b 272 1 The the DT cord-329553-93tbgn2b 272 2 cell cell NN cord-329553-93tbgn2b 272 3 surface surface NN cord-329553-93tbgn2b 272 4 expression expression NN cord-329553-93tbgn2b 272 5 of of IN cord-329553-93tbgn2b 272 6 each each DT cord-329553-93tbgn2b 272 7 fusion fusion NN cord-329553-93tbgn2b 272 8 protein protein NN cord-329553-93tbgn2b 272 9 was be VBD cord-329553-93tbgn2b 272 10 normalized normalize VBN cord-329553-93tbgn2b 272 11 to to IN cord-329553-93tbgn2b 272 12 that that DT cord-329553-93tbgn2b 272 13 of of IN cord-329553-93tbgn2b 272 14 DPPIV DPPIV NNP cord-329553-93tbgn2b 272 15 ( ( -LRB- cord-329553-93tbgn2b 272 16 arbitrarily arbitrarily RB cord-329553-93tbgn2b 272 17 defined define VBN cord-329553-93tbgn2b 272 18 as as IN cord-329553-93tbgn2b 272 19 100 100 CD cord-329553-93tbgn2b 272 20 % % NN cord-329553-93tbgn2b 272 21 ) ) -RRB- cord-329553-93tbgn2b 272 22 . . . cord-329553-93tbgn2b 273 1 Transfected transfecte VBN cord-329553-93tbgn2b 273 2 cells cell NNS cord-329553-93tbgn2b 274 1 A a LS cord-329553-93tbgn2b 274 2 , , , cord-329553-93tbgn2b 274 3 B B NNP cord-329553-93tbgn2b 274 4 , , , cord-329553-93tbgn2b 274 5 C C NNP cord-329553-93tbgn2b 274 6 , , , cord-329553-93tbgn2b 274 7 D D NNP cord-329553-93tbgn2b 274 8 , , , cord-329553-93tbgn2b 274 9 E E NNP cord-329553-93tbgn2b 274 10 , , , cord-329553-93tbgn2b 274 11 F F NNP cord-329553-93tbgn2b 274 12 , , , cord-329553-93tbgn2b 274 13 and and CC cord-329553-93tbgn2b 274 14 G g NN cord-329553-93tbgn2b 274 15 represent represent VBP cord-329553-93tbgn2b 274 16 cells cell NNS cord-329553-93tbgn2b 274 17 expressing express VBG cord-329553-93tbgn2b 274 18 DPPIV DPPIV NNP cord-329553-93tbgn2b 274 19 , , , cord-329553-93tbgn2b 274 20 S91 S91 NNP cord-329553-93tbgn2b 274 21 ) ) -RRB- cord-329553-93tbgn2b 274 22 , , , cord-329553-93tbgn2b 274 23 S27 S27 NNP cord-329553-93tbgn2b 274 24 - - HYPH cord-329553-93tbgn2b 274 25 331 331 CD cord-329553-93tbgn2b 275 1 , , , cord-329553-93tbgn2b 275 2 S33D s33d NN cord-329553-93tbgn2b 275 3 , , , cord-329553-93tbgn2b 275 4 S64D s64d NN cord-329553-93tbgn2b 275 5 , , , cord-329553-93tbgn2b 275 6 S10 S10 NNP cord-329553-93tbgn2b 275 7 - - HYPH cord-329553-93tbgn2b 275 8 331 331 CD cord-329553-93tbgn2b 275 9 ) ) -RRB- cord-329553-93tbgn2b 275 10 , , , cord-329553-93tbgn2b 275 11 and and CC cord-329553-93tbgn2b 275 12 S10 S10 NNP cord-329553-93tbgn2b 275 13 - - HYPH cord-329553-93tbgn2b 275 14 261 261 CD cord-329553-93tbgn2b 275 15 ) ) -RRB- cord-329553-93tbgn2b 275 16 , , , cord-329553-93tbgn2b 275 17 respectively respectively RB cord-329553-93tbgn2b 275 18 . . . cord-329553-93tbgn2b 276 1 pected pecte VBN cord-329553-93tbgn2b 276 2 if if IN cord-329553-93tbgn2b 276 3 their -PRON- PRP$ cord-329553-93tbgn2b 276 4 Golgi Golgi NNP cord-329553-93tbgn2b 276 5 localization localization NN cord-329553-93tbgn2b 276 6 was be VBD cord-329553-93tbgn2b 276 7 mediated mediate VBN cord-329553-93tbgn2b 276 8 by by IN cord-329553-93tbgn2b 276 9 initial initial JJ cord-329553-93tbgn2b 276 10 surface surface NN cord-329553-93tbgn2b 276 11 expression expression NN cord-329553-93tbgn2b 276 12 followed follow VBN cord-329553-93tbgn2b 276 13 by by IN cord-329553-93tbgn2b 276 14 specific specific JJ cord-329553-93tbgn2b 276 15 retrieval retrieval NN cord-329553-93tbgn2b 276 16 and and CC cord-329553-93tbgn2b 276 17 endocytosis endocytosis NN cord-329553-93tbgn2b 276 18 . . . cord-329553-93tbgn2b 277 1 This this DT cord-329553-93tbgn2b 277 2 was be VBD cord-329553-93tbgn2b 277 3 not not RB cord-329553-93tbgn2b 277 4 observed observe VBN cord-329553-93tbgn2b 277 5 for for IN cord-329553-93tbgn2b 277 6 either either CC cord-329553-93tbgn2b 277 7 S33D s33d JJ cord-329553-93tbgn2b 277 8 or or CC cord-329553-93tbgn2b 277 9 S10 S10 NNP cord-329553-93tbgn2b 277 10 - - HYPH cord-329553-93tbgn2b 277 11 261 261 CD cord-329553-93tbgn2b 277 12 . . . cord-329553-93tbgn2b 278 1 The the DT cord-329553-93tbgn2b 278 2 surface surface NN cord-329553-93tbgn2b 278 3 expression expression NN cord-329553-93tbgn2b 278 4 for for IN cord-329553-93tbgn2b 278 5 S33D s33d JJ cord-329553-93tbgn2b 278 6 and and CC cord-329553-93tbgn2b 278 7 S10 S10 NNP cord-329553-93tbgn2b 278 8 - - HYPH cord-329553-93tbgn2b 278 9 261 261 CD cord-329553-93tbgn2b 278 10 was be VBD cord-329553-93tbgn2b 278 11 undetectable undetectable JJ cord-329553-93tbgn2b 278 12 after after IN cord-329553-93tbgn2b 278 13 90 90 CD cord-329553-93tbgn2b 278 14 min min NN cord-329553-93tbgn2b 278 15 of of IN cord-329553-93tbgn2b 278 16 chase chase NN cord-329553-93tbgn2b 278 17 and and CC cord-329553-93tbgn2b 278 18 only only RB cord-329553-93tbgn2b 278 19 Nl-2%0 Nl-2%0 NNP cord-329553-93tbgn2b 278 20 ( ( -LRB- cord-329553-93tbgn2b 278 21 normalized normalize VBN cord-329553-93tbgn2b 278 22 to to IN cord-329553-93tbgn2b 278 23 DPPIV DPPIV NNP cord-329553-93tbgn2b 278 24 surface surface NN cord-329553-93tbgn2b 278 25 expression expression NN cord-329553-93tbgn2b 278 26 ) ) -RRB- cord-329553-93tbgn2b 278 27 was be VBD cord-329553-93tbgn2b 278 28 visible visible JJ cord-329553-93tbgn2b 278 29 after after IN cord-329553-93tbgn2b 278 30 2 2 CD cord-329553-93tbgn2b 278 31 h h NN cord-329553-93tbgn2b 278 32 of of IN cord-329553-93tbgn2b 278 33 chase chase NNP cord-329553-93tbgn2b 278 34 . . . cord-329553-93tbgn2b 279 1 Longer long RBR cord-329553-93tbgn2b 279 2 chase chase VB cord-329553-93tbgn2b 279 3 up up RP cord-329553-93tbgn2b 279 4 to to IN cord-329553-93tbgn2b 279 5 6 6 CD cord-329553-93tbgn2b 279 6 h h NN cord-329553-93tbgn2b 279 7 revealed reveal VBD cord-329553-93tbgn2b 279 8 a a DT cord-329553-93tbgn2b 279 9 slight slight JJ cord-329553-93tbgn2b 279 10 increase increase NN cord-329553-93tbgn2b 279 11 ( ( -LRB- cord-329553-93tbgn2b 279 12 up up IN cord-329553-93tbgn2b 279 13 to to TO cord-329553-93tbgn2b 279 14 4 4 CD cord-329553-93tbgn2b 279 15 - - SYM cord-329553-93tbgn2b 279 16 6 6 CD cord-329553-93tbgn2b 279 17 % % NN cord-329553-93tbgn2b 279 18 ) ) -RRB- cord-329553-93tbgn2b 279 19 in in IN cord-329553-93tbgn2b 279 20 surface surface NN cord-329553-93tbgn2b 279 21 expression expression NN cord-329553-93tbgn2b 279 22 for for IN cord-329553-93tbgn2b 279 23 S33D s33d JJ cord-329553-93tbgn2b 279 24 and and CC cord-329553-93tbgn2b 279 25 S10 S10 NNP cord-329553-93tbgn2b 279 26 - - HYPH cord-329553-93tbgn2b 279 27 2611 2611 CD cord-329553-93tbgn2b 280 1 This this DT cord-329553-93tbgn2b 280 2 was be VBD cord-329553-93tbgn2b 280 3 most most RBS cord-329553-93tbgn2b 280 4 likely likely JJ cord-329553-93tbgn2b 280 5 due due IN cord-329553-93tbgn2b 280 6 to to IN cord-329553-93tbgn2b 280 7 the the DT cord-329553-93tbgn2b 280 8 slight slight JJ cord-329553-93tbgn2b 280 9 leakiness leakiness NN cord-329553-93tbgn2b 280 10 in in IN cord-329553-93tbgn2b 280 11 their -PRON- PRP$ cord-329553-93tbgn2b 280 12 Golgi Golgi NNP cord-329553-93tbgn2b 280 13 retention retention NN cord-329553-93tbgn2b 280 14 . . . cord-329553-93tbgn2b 281 1 Interestingly interestingly RB cord-329553-93tbgn2b 281 2 , , , cord-329553-93tbgn2b 281 3 surface surface NN cord-329553-93tbgn2b 281 4 expression expression NN cord-329553-93tbgn2b 281 5 was be VBD cord-329553-93tbgn2b 281 6 undetectable undetectable JJ cord-329553-93tbgn2b 281 7 after after IN cord-329553-93tbgn2b 281 8 22 22 CD cord-329553-93tbgn2b 281 9 h h NN cord-329553-93tbgn2b 281 10 of of IN cord-329553-93tbgn2b 281 11 chase chase NNP cord-329553-93tbgn2b 281 12 , , , cord-329553-93tbgn2b 281 13 which which WDT cord-329553-93tbgn2b 281 14 couldbe couldbe RB cord-329553-93tbgn2b 281 15 explained explain VBD cord-329553-93tbgn2b 281 16 by by IN cord-329553-93tbgn2b 281 17 surface surface NN cord-329553-93tbgn2b 281 18 deg deg NNP cord-329553-93tbgn2b 281 19 - - : cord-329553-93tbgn2b 281 20 The the DT cord-329553-93tbgn2b 281 21 Journal Journal NNP cord-329553-93tbgn2b 281 22 of of IN cord-329553-93tbgn2b 281 23 Cell Cell NNP cord-329553-93tbgn2b 281 24 Biology Biology NNP cord-329553-93tbgn2b 281 25 , , , cord-329553-93tbgn2b 281 26 Volume volume NN cord-329553-93tbgn2b 281 27 117 117 CD cord-329553-93tbgn2b 281 28 , , , cord-329553-93tbgn2b 281 29 1992 1992 CD cord-329553-93tbgn2b 281 30 radation radation NN cord-329553-93tbgn2b 281 31 and/or and/or CC cord-329553-93tbgn2b 281 32 slow slow JJ cord-329553-93tbgn2b 281 33 internalization internalization NN cord-329553-93tbgn2b 281 34 with with IN cord-329553-93tbgn2b 281 35 the the DT cord-329553-93tbgn2b 281 36 bulk bulk JJ cord-329553-93tbgn2b 281 37 membrane membrane NN cord-329553-93tbgn2b 281 38 endocytosis endocytosis NN cord-329553-93tbgn2b 281 39 . . . cord-329553-93tbgn2b 282 1 These these DT cord-329553-93tbgn2b 282 2 results result NNS cord-329553-93tbgn2b 282 3 suggest suggest VBP cord-329553-93tbgn2b 282 4 that that IN cord-329553-93tbgn2b 282 5 both both CC cord-329553-93tbgn2b 282 6 S33D s33d JJ cord-329553-93tbgn2b 282 7 and and CC cord-329553-93tbgn2b 282 8 S10 S10 NNP cord-329553-93tbgn2b 282 9 - - HYPH cord-329553-93tbgn2b 282 10 26D 26d NN cord-329553-93tbgn2b 282 11 are be VBP cord-329553-93tbgn2b 282 12 specifically specifically RB cord-329553-93tbgn2b 282 13 retained retain VBN cord-329553-93tbgn2b 282 14 intracellularly intracellularly RB cord-329553-93tbgn2b 282 15 in in IN cord-329553-93tbgn2b 282 16 the the DT cord-329553-93tbgn2b 282 17 Golgi Golgi NNP cord-329553-93tbgn2b 282 18 apparatus apparatus NN cord-329553-93tbgn2b 282 19 . . . cord-329553-93tbgn2b 283 1 The the DT cord-329553-93tbgn2b 283 2 I7-Residue I7-Residue NNP cord-329553-93tbgn2b 283 3 Transmembrane Transmembrane NNP cord-329553-93tbgn2b 283 4 Domain Domain NNP cord-329553-93tbgn2b 283 5 of of IN cord-329553-93tbgn2b 283 6 ST ST NNP cord-329553-93tbgn2b 283 7 Is be VBZ cord-329553-93tbgn2b 283 8 Sufcientfor Sufcientfor NNP cord-329553-93tbgn2b 283 9 Golgi Golgi NNP cord-329553-93tbgn2b 283 10 Localization Localization NNP cord-329553-93tbgn2b 284 1 Previous previous JJ cord-329553-93tbgn2b 284 2 observations observation NNS cord-329553-93tbgn2b 284 3 have have VBP cord-329553-93tbgn2b 284 4 shown show VBN cord-329553-93tbgn2b 284 5 that that IN cord-329553-93tbgn2b 284 6 the the DT cord-329553-93tbgn2b 284 7 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 284 8 re re NNP cord-329553-93tbgn2b 284 9 - - NN cord-329553-93tbgn2b 284 10 gion gion NNP cord-329553-93tbgn2b 284 11 of of IN cord-329553-93tbgn2b 284 12 ST ST NNP cord-329553-93tbgn2b 284 13 is be VBZ cord-329553-93tbgn2b 284 14 required require VBN cord-329553-93tbgn2b 284 15 for for IN cord-329553-93tbgn2b 284 16 membrane membrane NN cord-329553-93tbgn2b 284 17 anchorage anchorage NNP cord-329553-93tbgn2b 284 18 and and CC cord-329553-93tbgn2b 284 19 Golgi Golgi NNP cord-329553-93tbgn2b 284 20 localization localization NN cord-329553-93tbgn2b 284 21 Jamieson Jamieson NNP cord-329553-93tbgn2b 284 22 , , , cord-329553-93tbgn2b 284 23 1988 1988 CD cord-329553-93tbgn2b 284 24 , , , cord-329553-93tbgn2b 284 25 1989 1989 CD cord-329553-93tbgn2b 284 26 ; ; : cord-329553-93tbgn2b 284 27 Paulson Paulson NNP cord-329553-93tbgn2b 284 28 and and CC cord-329553-93tbgn2b 284 29 Colley Colley NNP cord-329553-93tbgn2b 284 30 , , , cord-329553-93tbgn2b 284 31 1989 1989 CD cord-329553-93tbgn2b 284 32 ; ; : cord-329553-93tbgn2b 284 33 Colley Colley NNP cord-329553-93tbgn2b 284 34 et et NNP cord-329553-93tbgn2b 284 35 al al NNP cord-329553-93tbgn2b 284 36 . . NNP cord-329553-93tbgn2b 284 37 , , , cord-329553-93tbgn2b 284 38 1989 1989 CD cord-329553-93tbgn2b 284 39 ; ; : cord-329553-93tbgn2b 284 40 Weinstein Weinstein NNP cord-329553-93tbgn2b 284 41 et et NNP cord-329553-93tbgn2b 284 42 al al NNP cord-329553-93tbgn2b 284 43 . . NNP cord-329553-93tbgn2b 284 44 , , , cord-329553-93tbgn2b 284 45 1987 1987 CD cord-329553-93tbgn2b 284 46 ) ) -RRB- cord-329553-93tbgn2b 284 47 . . . cord-329553-93tbgn2b 285 1 We -PRON- PRP cord-329553-93tbgn2b 285 2 thus thus RB cord-329553-93tbgn2b 285 3 constructed construct VBD cord-329553-93tbgn2b 285 4 a a DT cord-329553-93tbgn2b 285 5 series series NN cord-329553-93tbgn2b 285 6 of of IN cord-329553-93tbgn2b 285 7 chimeric chimeric JJ cord-329553-93tbgn2b 285 8 cDNAs cdna NNS cord-329553-93tbgn2b 285 9 encoding encode VBG cord-329553-93tbgn2b 285 10 fusion fusion NN cord-329553-93tbgn2b 285 11 proteins protein NNS cord-329553-93tbgn2b 285 12 in in IN cord-329553-93tbgn2b 285 13 which which WDT cord-329553-93tbgn2b 285 14 the the DT cord-329553-93tbgn2b 285 15 N112 N112 NNP cord-329553-93tbgn2b 285 16 -terminal -terminal JJ cord-329553-93tbgn2b 285 17 ST ST NNP cord-329553-93tbgn2b 285 18 sequence sequence NN cord-329553-93tbgn2b 285 19 of of IN cord-329553-93tbgn2b 285 20 different different JJ cord-329553-93tbgn2b 285 21 sizes size NNS cord-329553-93tbgn2b 285 22 were be VBD cord-329553-93tbgn2b 285 23 fused fuse VBN cord-329553-93tbgn2b 285 24 to to IN cord-329553-93tbgn2b 285 25 the the DT cord-329553-93tbgn2b 285 26 ectodomain ectodomain NN cord-329553-93tbgn2b 285 27 of of IN cord-329553-93tbgn2b 285 28 a a DT cord-329553-93tbgn2b 285 29 cell cell NN cord-329553-93tbgn2b 285 30 surface surface NN cord-329553-93tbgn2b 285 31 protein protein NN cord-329553-93tbgn2b 285 32 DPPIV DPPIV NNP cord-329553-93tbgn2b 285 33 ( ( -LRB- cord-329553-93tbgn2b 285 34 Hong Hong NNP cord-329553-93tbgn2b 285 35 and and CC cord-329553-93tbgn2b 285 36 Doyle Doyle NNP cord-329553-93tbgn2b 285 37 , , , cord-329553-93tbgn2b 285 38 1990 1990 CD cord-329553-93tbgn2b 285 39 ; ; : cord-329553-93tbgn2b 285 40 Ogato Ogato NNP cord-329553-93tbgn2b 285 41 et et NNP cord-329553-93tbgn2b 285 42 al al NNP cord-329553-93tbgn2b 285 43 . . NNP cord-329553-93tbgn2b 285 44 , , , cord-329553-93tbgn2b 285 45 1989 1989 CD cord-329553-93tbgn2b 285 46 ; ; : cord-329553-93tbgn2b 286 1 Low low JJ cord-329553-93tbgn2b 286 2 et et FW cord-329553-93tbgn2b 286 3 al al NNP cord-329553-93tbgn2b 286 4 . . NNP cord-329553-93tbgn2b 286 5 , , , cord-329553-93tbgn2b 286 6 1991a 1991a CD cord-329553-93tbgn2b 286 7 , , , cord-329553-93tbgn2b 286 8 ó ó NNP cord-329553-93tbgn2b 286 9 ) ) -RRB- cord-329553-93tbgn2b 286 10 . . . cord-329553-93tbgn2b 287 1 When when WRB cord-329553-93tbgn2b 287 2 expressed express VBN cord-329553-93tbgn2b 287 3 in in IN cord-329553-93tbgn2b 287 4 transfected transfecte VBN cord-329553-93tbgn2b 287 5 MDCK MDCK NNP cord-329553-93tbgn2b 287 6 cells cell NNS cord-329553-93tbgn2b 287 7 , , , cord-329553-93tbgn2b 287 8 fusion fusion NN cord-329553-93tbgn2b 287 9 proteins protein NNS cord-329553-93tbgn2b 287 10 bearing bear VBG cord-329553-93tbgn2b 287 11 NHZ nhz NN cord-329553-93tbgn2b 287 12 - - HYPH cord-329553-93tbgn2b 287 13 terminal terminal NN cord-329553-93tbgn2b 287 14 64- 64- NNP cord-329553-93tbgn2b 287 15 , , , cord-329553-93tbgn2b 287 16 51- 51- NNP cord-329553-93tbgn2b 287 17 , , , cord-329553-93tbgn2b 287 18 46- 46- CD cord-329553-93tbgn2b 287 19 , , , cord-329553-93tbgn2b 287 20 40- 40- CD cord-329553-93tbgn2b 287 21 , , , cord-329553-93tbgn2b 287 22 and and CC cord-329553-93tbgn2b 287 23 33-residue 33-residue CD cord-329553-93tbgn2b 287 24 ST ST NNP cord-329553-93tbgn2b 287 25 sequences sequence NNS cord-329553-93tbgn2b 287 26 were be VBD cord-329553-93tbgn2b 287 27 all all DT cord-329553-93tbgn2b 287 28 localized localize VBN cord-329553-93tbgn2b 287 29 to to IN cord-329553-93tbgn2b 287 30 the the DT cord-329553-93tbgn2b 287 31 Golgi Golgi NNP cord-329553-93tbgn2b 287 32 apparatus apparatus NN cord-329553-93tbgn2b 287 33 as as IN cord-329553-93tbgn2b 287 34 assessed assess VBN cord-329553-93tbgn2b 287 35 by by IN cord-329553-93tbgn2b 287 36 indirect indirect JJ cord-329553-93tbgn2b 287 37 fluorescence fluorescence NN cord-329553-93tbgn2b 287 38 microscopy microscopy NN cord-329553-93tbgn2b 287 39 and and CC cord-329553-93tbgn2b 287 40 other other JJ cord-329553-93tbgn2b 287 41 biochemical biochemical JJ cord-329553-93tbgn2b 287 42 methods method NNS cord-329553-93tbgn2b 287 43 . . . cord-329553-93tbgn2b 288 1 The the DT cord-329553-93tbgn2b 288 2 results result NNS cord-329553-93tbgn2b 288 3 with with IN cord-329553-93tbgn2b 288 4 two two CD cord-329553-93tbgn2b 288 5 fusion fusion NN cord-329553-93tbgn2b 288 6 proteins protein NNS cord-329553-93tbgn2b 288 7 , , , cord-329553-93tbgn2b 288 8 S33D s33d NN cord-329553-93tbgn2b 288 9 and and CC cord-329553-93tbgn2b 288 10 S64D s64d NN cord-329553-93tbgn2b 288 11 , , , cord-329553-93tbgn2b 288 12 are be VBP cord-329553-93tbgn2b 288 13 presented present VBN cord-329553-93tbgn2b 288 14 in in IN cord-329553-93tbgn2b 288 15 this this DT cord-329553-93tbgn2b 288 16 report report NN cord-329553-93tbgn2b 288 17 . . . cord-329553-93tbgn2b 289 1 Pulse pulse NN cord-329553-93tbgn2b 289 2 - - HYPH cord-329553-93tbgn2b 289 3 chase chase NN cord-329553-93tbgn2b 289 4 experiments experiment NNS cord-329553-93tbgn2b 289 5 demonstrated demonstrate VBD cord-329553-93tbgn2b 289 6 that that IN cord-329553-93tbgn2b 289 7 both both CC cord-329553-93tbgn2b 289 8 S33D s33d NN cord-329553-93tbgn2b 289 9 and and CC cord-329553-93tbgn2b 289 10 S64D S64D NNP cord-329553-93tbgn2b 289 11 wereefficiently wereefficiently RB cord-329553-93tbgn2b 289 12 transported transport VBD cord-329553-93tbgn2b 289 13 from from IN cord-329553-93tbgn2b 289 14 the the DT cord-329553-93tbgn2b 289 15 ER ER NNP cord-329553-93tbgn2b 289 16 to to IN cord-329553-93tbgn2b 289 17 the the DT cord-329553-93tbgn2b 289 18 Golgi Golgi NNP cord-329553-93tbgn2b 289 19 apparatus apparatus NN cord-329553-93tbgn2b 289 20 and and CC cord-329553-93tbgn2b 289 21 have have VBP cord-329553-93tbgn2b 289 22 passed pass VBN cord-329553-93tbgn2b 289 23 beyond beyond IN cord-329553-93tbgn2b 289 24 the the DT cord-329553-93tbgn2b 289 25 medial medial JJ cord-329553-93tbgn2b 289 26 - - HYPH cord-329553-93tbgn2b 289 27 Golgi Golgi NNP cord-329553-93tbgn2b 289 28 cisternae cisternae NN cord-329553-93tbgn2b 289 29 . . . cord-329553-93tbgn2b 290 1 Affinity affinity NN cord-329553-93tbgn2b 290 2 chromatography chromatography NN cord-329553-93tbgn2b 290 3 with with IN cord-329553-93tbgn2b 290 4 several several JJ cord-329553-93tbgn2b 290 5 lectin lectin NN cord-329553-93tbgn2b 290 6 - - HYPH cord-329553-93tbgn2b 290 7 agaroses agarose NNS cord-329553-93tbgn2b 290 8 demonstrated demonstrate VBD cord-329553-93tbgn2b 290 9 that that IN cord-329553-93tbgn2b 290 10 both both CC cord-329553-93tbgn2b 290 11 S33D s33d NN cord-329553-93tbgn2b 290 12 and and CC cord-329553-93tbgn2b 290 13 S64D s64d NN cord-329553-93tbgn2b 290 14 were be VBD cord-329553-93tbgn2b 290 15 extensively extensively RB cord-329553-93tbgn2b 290 16 modified modify VBN cord-329553-93tbgn2b 290 17 by by IN cord-329553-93tbgn2b 290 18 GT GT NNP cord-329553-93tbgn2b 290 19 and and CC cord-329553-93tbgn2b 290 20 ST ST NNP cord-329553-93tbgn2b 290 21 . . . cord-329553-93tbgn2b 291 1 Since since IN cord-329553-93tbgn2b 291 2 GT GT NNP cord-329553-93tbgn2b 291 3 has have VBZ cord-329553-93tbgn2b 291 4 been be VBN cord-329553-93tbgn2b 291 5 previously previously RB cord-329553-93tbgn2b 291 6 localized localize VBN cord-329553-93tbgn2b 291 7 to to IN cord-329553-93tbgn2b 291 8 the the DT cord-329553-93tbgn2b 291 9 trans trans NNP cord-329553-93tbgn2b 291 10 - - NNP cord-329553-93tbgn2b 291 11 Golgi Golgi NNP cord-329553-93tbgn2b 291 12 cisternae cisternae NN cord-329553-93tbgn2b 291 13 ( ( -LRB- cord-329553-93tbgn2b 291 14 Roth Roth NNP cord-329553-93tbgn2b 291 15 and and CC cord-329553-93tbgn2b 291 16 Berger Berger NNP cord-329553-93tbgn2b 291 17 , , , cord-329553-93tbgn2b 291 18 1982 1982 CD cord-329553-93tbgn2b 291 19 ) ) -RRB- cord-329553-93tbgn2b 291 20 and and CC cord-329553-93tbgn2b 291 21 ST ST NNP cord-329553-93tbgn2b 291 22 has have VBZ cord-329553-93tbgn2b 291 23 been be VBN cord-329553-93tbgn2b 291 24 localized localize VBN cord-329553-93tbgn2b 291 25 to to IN cord-329553-93tbgn2b 291 26 the the DT cord-329553-93tbgn2b 291 27 trans trans NNP cord-329553-93tbgn2b 291 28 - - NNP cord-329553-93tbgn2b 291 29 Golgi Golgi NNP cord-329553-93tbgn2b 291 30 cisternae cisternae NNP cord-329553-93tbgn2b 291 31 and and CC cord-329553-93tbgn2b 291 32 TGN TGN NNP cord-329553-93tbgn2b 291 33 ( ( -LRB- cord-329553-93tbgn2b 291 34 Both both CC cord-329553-93tbgn2b 291 35 et et FW cord-329553-93tbgn2b 291 36 al al NNP cord-329553-93tbgn2b 291 37 . . NNP cord-329553-93tbgn2b 291 38 , , , cord-329553-93tbgn2b 291 39 1985 1985 CD cord-329553-93tbgn2b 291 40 ) ) -RRB- cord-329553-93tbgn2b 291 41 , , , cord-329553-93tbgn2b 291 42 these these DT cord-329553-93tbgn2b 291 43 results result NNS cord-329553-93tbgn2b 291 44 suggest suggest VBP cord-329553-93tbgn2b 291 45 that that IN cord-329553-93tbgn2b 291 46 S33D s33d NN cord-329553-93tbgn2b 291 47 and and CC cord-329553-93tbgn2b 291 48 S64D s64d NN cord-329553-93tbgn2b 291 49 are be VBP cord-329553-93tbgn2b 291 50 localized localize VBN cord-329553-93tbgn2b 291 51 in in IN cord-329553-93tbgn2b 291 52 the the DT cord-329553-93tbgn2b 291 53 trans trans NNP cord-329553-93tbgn2b 291 54 - - NNP cord-329553-93tbgn2b 291 55 Golgi Golgi NNP cord-329553-93tbgn2b 291 56 cisternae cisternae NNP cord-329553-93tbgn2b 291 57 and/or and/or CC cord-329553-93tbgn2b 291 58 the the DT cord-329553-93tbgn2b 291 59 TGN TGN NNP cord-329553-93tbgn2b 291 60 . . . cord-329553-93tbgn2b 292 1 Because because IN cord-329553-93tbgn2b 292 2 the the DT cord-329553-93tbgn2b 292 3 actual actual JJ cord-329553-93tbgn2b 292 4 localization localization NN cord-329553-93tbgn2b 292 5 of of IN cord-329553-93tbgn2b 292 6 ST ST NNP cord-329553-93tbgn2b 292 7 in in IN cord-329553-93tbgn2b 292 8 MDCK MDCK NNP cord-329553-93tbgn2b 292 9 cells cell NNS cord-329553-93tbgn2b 292 10 has have VBZ cord-329553-93tbgn2b 292 11 not not RB cord-329553-93tbgn2b 292 12 been be VBN cord-329553-93tbgn2b 292 13 established establish VBN cord-329553-93tbgn2b 292 14 , , , cord-329553-93tbgn2b 292 15 our -PRON- PRP$ cord-329553-93tbgn2b 292 16 conclusion conclusion NN cord-329553-93tbgn2b 292 17 for for IN cord-329553-93tbgn2b 292 18 the the DT cord-329553-93tbgn2b 292 19 trans trans NNP cord-329553-93tbgn2b 292 20 - - NNP cord-329553-93tbgn2b 292 21 Golgi Golgi NNP cord-329553-93tbgn2b 292 22 cisternae cisternae NNP cord-329553-93tbgn2b 292 23 and/or and/or CC cord-329553-93tbgn2b 292 24 TGN TGN NNP cord-329553-93tbgn2b 292 25 localization localization NN cord-329553-93tbgn2b 292 26 of of IN cord-329553-93tbgn2b 292 27 S33D S33D NNP cord-329553-93tbgn2b 292 28 and and CC cord-329553-93tbgn2b 292 29 D64D D64D NNP cord-329553-93tbgn2b 292 30 is be VBZ cord-329553-93tbgn2b 292 31 based base VBN cord-329553-93tbgn2b 292 32 on on IN cord-329553-93tbgn2b 292 33 the the DT cord-329553-93tbgn2b 292 34 assumption assumption NN cord-329553-93tbgn2b 292 35 that that IN cord-329553-93tbgn2b 292 36 ST ST NNP cord-329553-93tbgn2b 292 37 is be VBZ cord-329553-93tbgn2b 292 38 localized localize VBN cord-329553-93tbgn2b 292 39 in in IN cord-329553-93tbgn2b 292 40 the the DT cord-329553-93tbgn2b 292 41 same same JJ cord-329553-93tbgn2b 292 42 compartments compartment NNS cord-329553-93tbgn2b 292 43 in in IN cord-329553-93tbgn2b 292 44 MDCK MDCK NNP cord-329553-93tbgn2b 292 45 cells cell NNS cord-329553-93tbgn2b 292 46 . . . cord-329553-93tbgn2b 293 1 Our -PRON- PRP$ cord-329553-93tbgn2b 293 2 results result NNS cord-329553-93tbgn2b 293 3 , , , cord-329553-93tbgn2b 293 4 nevertheless nevertheless RB cord-329553-93tbgn2b 293 5 , , , cord-329553-93tbgn2b 293 6 demonstrated demonstrate VBD cord-329553-93tbgn2b 293 7 that that IN cord-329553-93tbgn2b 293 8 S33D s33d NN cord-329553-93tbgn2b 293 9 and and CC cord-329553-93tbgn2b 293 10 S64D s64d NN cord-329553-93tbgn2b 293 11 are be VBP cord-329553-93tbgn2b 293 12 localized localize VBN cord-329553-93tbgn2b 293 13 in in IN cord-329553-93tbgn2b 293 14 the the DT cord-329553-93tbgn2b 293 15 Golgi Golgi NNP cord-329553-93tbgn2b 293 16 subcompartments subcompartment NNS cord-329553-93tbgn2b 293 17 in in IN cord-329553-93tbgn2b 293 18 which which WDT cord-329553-93tbgn2b 293 19 they -PRON- PRP cord-329553-93tbgn2b 293 20 have have VBP cord-329553-93tbgn2b 293 21 extensive extensive JJ cord-329553-93tbgn2b 293 22 interaction interaction NN cord-329553-93tbgn2b 293 23 with with IN cord-329553-93tbgn2b 293 24 endogenous endogenous JJ cord-329553-93tbgn2b 293 25 GT GT NNP cord-329553-93tbgn2b 293 26 and and CC cord-329553-93tbgn2b 293 27 ST ST NNP cord-329553-93tbgn2b 293 28 . . . cord-329553-93tbgn2b 294 1 Since since IN cord-329553-93tbgn2b 294 2 S33D s33d RB cord-329553-93tbgn2b 294 3 and and CC cord-329553-93tbgn2b 294 4 S64D s64d NN cord-329553-93tbgn2b 294 5 ( ( -LRB- cord-329553-93tbgn2b 294 6 as as RB cord-329553-93tbgn2b 294 7 well well RB cord-329553-93tbgn2b 294 8 as as IN cord-329553-93tbgn2b 294 9 S51D s51d NN cord-329553-93tbgn2b 294 10 , , , cord-329553-93tbgn2b 294 11 S46D S46D NNP cord-329553-93tbgn2b 294 12 , , , cord-329553-93tbgn2b 294 13 and and CC cord-329553-93tbgn2b 294 14 S40D s40d NN cord-329553-93tbgn2b 294 15 ) ) -RRB- cord-329553-93tbgn2b 294 16 were be VBD cord-329553-93tbgn2b 294 17 localized localize VBN cord-329553-93tbgn2b 294 18 to to IN cord-329553-93tbgn2b 294 19 the the DT cord-329553-93tbgn2b 294 20 Golgi Golgi NNP cord-329553-93tbgn2b 294 21 apparatus apparatus NN cord-329553-93tbgn2b 294 22 with with IN cord-329553-93tbgn2b 294 23 comparable comparable JJ cord-329553-93tbgn2b 294 24 efficiencies efficiency NNS cord-329553-93tbgn2b 294 25 , , , cord-329553-93tbgn2b 294 26 it -PRON- PRP cord-329553-93tbgn2b 294 27 was be VBD cord-329553-93tbgn2b 294 28 concluded conclude VBN cord-329553-93tbgn2b 294 29 that that IN cord-329553-93tbgn2b 294 30 the the DT cord-329553-93tbgn2b 294 31 N112-terminal n112-terminal NN cord-329553-93tbgn2b 294 32 33-Wong 33-wong CD cord-329553-93tbgn2b 294 33 et et NNP cord-329553-93tbgn2b 294 34 al al NNP cord-329553-93tbgn2b 294 35 . . . cord-329553-93tbgn2b 295 1 Signal signal NN cord-329553-93tbgn2b 295 2 for for IN cord-329553-93tbgn2b 295 3 Golgi Golgi NNP cord-329553-93tbgn2b 295 4 Retention Retention NNP cord-329553-93tbgn2b 295 5 Figure Figure NNP cord-329553-93tbgn2b 295 6 8 8 CD cord-329553-93tbgn2b 295 7 . . . cord-329553-93tbgn2b 296 1 S33D s33d JJ cord-329553-93tbgn2b 296 2 and and CC cord-329553-93tbgn2b 296 3 S10 S10 NNP cord-329553-93tbgn2b 296 4 - - HYPH cord-329553-93tbgn2b 296 5 26D 26d NN cord-329553-93tbgn2b 296 6 are be VBP cord-329553-93tbgn2b 296 7 predominantly predominantly RB cord-329553-93tbgn2b 296 8 retained retain VBN cord-329553-93tbgn2b 296 9 intracellularly intracellularly RB cord-329553-93tbgn2b 296 10 . . . cord-329553-93tbgn2b 297 1 Cells cell NNS cord-329553-93tbgn2b 297 2 expressing express VBG cord-329553-93tbgn2b 297 3 DPPIV DPPIV NNP cord-329553-93tbgn2b 297 4 , , , cord-329553-93tbgn2b 297 5 S33D s33d NN cord-329553-93tbgn2b 297 6 , , , cord-329553-93tbgn2b 297 7 or or CC cord-329553-93tbgn2b 297 8 S10 S10 NNP cord-329553-93tbgn2b 297 9 - - HYPH cord-329553-93tbgn2b 297 10 26D 26d NN cord-329553-93tbgn2b 297 11 were be VBD cord-329553-93tbgn2b 297 12 pulse pulse NN cord-329553-93tbgn2b 297 13 - - HYPH cord-329553-93tbgn2b 297 14 labeled label VBN cord-329553-93tbgn2b 297 15 with with IN cord-329553-93tbgn2b 297 16 [ [ -LRB- cord-329553-93tbgn2b 297 17 35S]methionine 35s]methionine CD cord-329553-93tbgn2b 297 18 for for IN cord-329553-93tbgn2b 297 19 30 30 CD cord-329553-93tbgn2b 297 20 min min NN cord-329553-93tbgn2b 297 21 followed follow VBN cord-329553-93tbgn2b 297 22 by by IN cord-329553-93tbgn2b 297 23 a a DT cord-329553-93tbgn2b 297 24 series series NN cord-329553-93tbgn2b 297 25 of of IN cord-329553-93tbgn2b 297 26 chase chase NN cord-329553-93tbgn2b 297 27 intervals interval NNS cord-329553-93tbgn2b 297 28 as as IN cord-329553-93tbgn2b 297 29 indicated indicate VBN cord-329553-93tbgn2b 297 30 . . . cord-329553-93tbgn2b 298 1 The the DT cord-329553-93tbgn2b 298 2 cell cell NN cord-329553-93tbgn2b 298 3 surface surface NN cord-329553-93tbgn2b 298 4 proteins protein NNS cord-329553-93tbgn2b 298 5 were be VBD cord-329553-93tbgn2b 298 6 biotinylated biotinylate VBN cord-329553-93tbgn2b 298 7 at at IN cord-329553-93tbgn2b 298 8 each each DT cord-329553-93tbgn2b 298 9 chase chase NN cord-329553-93tbgn2b 298 10 point point NN cord-329553-93tbgn2b 298 11 . . . cord-329553-93tbgn2b 299 1 Cell cell NN cord-329553-93tbgn2b 299 2 lysates lysate NNS cord-329553-93tbgn2b 299 3 prepared prepare VBD cord-329553-93tbgn2b 299 4 from from IN cord-329553-93tbgn2b 299 5 each each DT cord-329553-93tbgn2b 299 6 chase chase NN cord-329553-93tbgn2b 299 7 interval interval NN cord-329553-93tbgn2b 299 8 were be VBD cord-329553-93tbgn2b 299 9 immunoprecipitated immunoprecipitate VBN cord-329553-93tbgn2b 299 10 . . . cord-329553-93tbgn2b 300 1 One one CD cord-329553-93tbgn2b 300 2 tenth tenth NN cord-329553-93tbgn2b 300 3 of of IN cord-329553-93tbgn2b 300 4 the the DT cord-329553-93tbgn2b 300 5 immunocomplex immunocomplex NN cord-329553-93tbgn2b 300 6 was be VBD cord-329553-93tbgn2b 300 7 analyzed analyze VBN cord-329553-93tbgn2b 300 8 as as IN cord-329553-93tbgn2b 300 9 a a DT cord-329553-93tbgn2b 300 10 measure measure NN cord-329553-93tbgn2b 300 11 of of IN cord-329553-93tbgn2b 300 12 total total NN cord-329553-93tbgn2b 300 13 newly newly RB cord-329553-93tbgn2b 300 14 made make VBN cord-329553-93tbgn2b 300 15 protein protein NN cord-329553-93tbgn2b 300 16 ( ( -LRB- cord-329553-93tbgn2b 300 17 1/10 1/10 CD cord-329553-93tbgn2b 300 18 Total Total NNP cord-329553-93tbgn2b 300 19 ) ) -RRB- cord-329553-93tbgn2b 300 20 . . . cord-329553-93tbgn2b 301 1 The the DT cord-329553-93tbgn2b 301 2 remaining remain VBG cord-329553-93tbgn2b 301 3 nine nine CD cord-329553-93tbgn2b 301 4 tenths tenth NNS cord-329553-93tbgn2b 301 5 was be VBD cord-329553-93tbgn2b 301 6 absorbed absorb VBN cord-329553-93tbgn2b 301 7 to to IN cord-329553-93tbgn2b 301 8 streptavidinagarose streptavidinagarose NNS cord-329553-93tbgn2b 301 9 to to TO cord-329553-93tbgn2b 301 10 recover recover VB cord-329553-93tbgn2b 301 11 the the DT cord-329553-93tbgn2b 301 12 surface surface NN cord-329553-93tbgn2b 301 13 biotinylated biotinylate VBN cord-329553-93tbgn2b 301 14 proteins protein NNS cord-329553-93tbgn2b 301 15 as as IN cord-329553-93tbgn2b 301 16 a a DT cord-329553-93tbgn2b 301 17 measure measure NN cord-329553-93tbgn2b 301 18 of of IN cord-329553-93tbgn2b 301 19 surface surface NN cord-329553-93tbgn2b 301 20 expression expression NN cord-329553-93tbgn2b 301 21 ( ( -LRB- cord-329553-93tbgn2b 301 22 Surface surface NN cord-329553-93tbgn2b 301 23 ) ) -RRB- cord-329553-93tbgn2b 301 24 . . . cord-329553-93tbgn2b 302 1 Intense intense JJ cord-329553-93tbgn2b 302 2 surface surface NN cord-329553-93tbgn2b 302 3 expression expression NN cord-329553-93tbgn2b 302 4 was be VBD cord-329553-93tbgn2b 302 5 detected detect VBN cord-329553-93tbgn2b 302 6 for for IN cord-329553-93tbgn2b 302 7 DPPIV DPPIV NNP cord-329553-93tbgn2b 302 8 while while IN cord-329553-93tbgn2b 302 9 the the DT cord-329553-93tbgn2b 302 10 surface surface NN cord-329553-93tbgn2b 302 11 expression expression NN cord-329553-93tbgn2b 302 12 ofthe ofthe DT cord-329553-93tbgn2b 302 13 two two CD cord-329553-93tbgn2b 302 14 fusion fusion NN cord-329553-93tbgn2b 302 15 proteins protein NNS cord-329553-93tbgn2b 302 16 was be VBD cord-329553-93tbgn2b 302 17 hardly hardly RB cord-329553-93tbgn2b 302 18 detectable detectable JJ cord-329553-93tbgn2b 302 19 . . . cord-329553-93tbgn2b 303 1 residue residue JJ cord-329553-93tbgn2b 303 2 ST ST NNP cord-329553-93tbgn2b 303 3 sequence sequence NN cord-329553-93tbgn2b 303 4 is be VBZ cord-329553-93tbgn2b 303 5 sufficient sufficient JJ cord-329553-93tbgn2b 303 6 for for IN cord-329553-93tbgn2b 303 7 Golgi Golgi NNP cord-329553-93tbgn2b 303 8 localization localization NN cord-329553-93tbgn2b 303 9 and and CC cord-329553-93tbgn2b 303 10 that that IN cord-329553-93tbgn2b 303 11 the the DT cord-329553-93tbgn2b 303 12 stem stem NN cord-329553-93tbgn2b 303 13 region region NN cord-329553-93tbgn2b 303 14 from from IN cord-329553-93tbgn2b 303 15 residues residue NNS cord-329553-93tbgn2b 303 16 34 34 CD cord-329553-93tbgn2b 303 17 to to TO cord-329553-93tbgn2b 303 18 64 64 CD cord-329553-93tbgn2b 303 19 is be VBZ cord-329553-93tbgn2b 303 20 not not RB cord-329553-93tbgn2b 303 21 required require VBN cord-329553-93tbgn2b 303 22 for for IN cord-329553-93tbgn2b 303 23 Golgi Golgi NNP cord-329553-93tbgn2b 303 24 localization localization NN cord-329553-93tbgn2b 303 25 . . . cord-329553-93tbgn2b 304 1 The the DT cord-329553-93tbgn2b 304 2 N112-terminal n112-terminal JJ cord-329553-93tbgn2b 304 3 33-residue 33-residue CD cord-329553-93tbgn2b 304 4 sequence sequence NN cord-329553-93tbgn2b 304 5 was be VBD cord-329553-93tbgn2b 304 6 further further RB cord-329553-93tbgn2b 304 7 divided divide VBN cord-329553-93tbgn2b 304 8 into into IN cord-329553-93tbgn2b 304 9 three three CD cord-329553-93tbgn2b 304 10 regions region NNS cord-329553-93tbgn2b 304 11 : : : cord-329553-93tbgn2b 304 12 the the DT cord-329553-93tbgn2b 304 13 9-residue 9-residue CD cord-329553-93tbgn2b 304 14 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 304 15 domain domain NN cord-329553-93tbgn2b 304 16 , , , cord-329553-93tbgn2b 304 17 the the DT cord-329553-93tbgn2b 304 18 17-residue 17-residue CD cord-329553-93tbgn2b 304 19 transmembrane transmembrane NN cord-329553-93tbgn2b 304 20 domain domain NN cord-329553-93tbgn2b 304 21 , , , cord-329553-93tbgn2b 304 22 and and CC cord-329553-93tbgn2b 304 23 the the DT cord-329553-93tbgn2b 304 24 7-residue 7-residue CD cord-329553-93tbgn2b 304 25 sequence sequence NN cord-329553-93tbgn2b 304 26 flanking flank VBG cord-329553-93tbgn2b 304 27 the the DT cord-329553-93tbgn2b 304 28 COOH COOH NNP cord-329553-93tbgn2b 304 29 - - HYPH cord-329553-93tbgn2b 304 30 terminal terminal JJ cord-329553-93tbgn2b 304 31 side side NN cord-329553-93tbgn2b 304 32 of of IN cord-329553-93tbgn2b 304 33 the the DT cord-329553-93tbgn2b 304 34 transmembrane transmembrane NN cord-329553-93tbgn2b 304 35 domain domain NN cord-329553-93tbgn2b 304 36 . . . cord-329553-93tbgn2b 305 1 More More JJR cord-329553-93tbgn2b 305 2 chimeric chimeric JJ cord-329553-93tbgn2b 305 3 cDNAs cdna NNS cord-329553-93tbgn2b 305 4 were be VBD cord-329553-93tbgn2b 305 5 thus thus RB cord-329553-93tbgn2b 305 6 constructed construct VBN cord-329553-93tbgn2b 305 7 encoding encode VBG cord-329553-93tbgn2b 305 8 additional additional JJ cord-329553-93tbgn2b 305 9 fusion fusion NN cord-329553-93tbgn2b 305 10 proteins protein NNS cord-329553-93tbgn2b 305 11 . . . cord-329553-93tbgn2b 306 1 The the DT cord-329553-93tbgn2b 306 2 transmembrane transmembrane NN cord-329553-93tbgn2b 306 3 domain domain NN cord-329553-93tbgn2b 306 4 alone alone RB cord-329553-93tbgn2b 306 5 ( ( -LRB- cord-329553-93tbgn2b 306 6 in in IN cord-329553-93tbgn2b 306 7 S10 S10 NNP cord-329553-93tbgn2b 306 8 - - HYPH cord-329553-93tbgn2b 306 9 26D 26d NN cord-329553-93tbgn2b 306 10 ) ) -RRB- cord-329553-93tbgn2b 306 11 or or CC cord-329553-93tbgn2b 306 12 in in IN cord-329553-93tbgn2b 306 13 combination combination NN cord-329553-93tbgn2b 306 14 with with IN cord-329553-93tbgn2b 306 15 its -PRON- PRP$ cord-329553-93tbgn2b 306 16 000Hterminal 000hterminal JJ cord-329553-93tbgn2b 306 17 flanking flanking JJ cord-329553-93tbgn2b 306 18 sequence sequence NN cord-329553-93tbgn2b 306 19 ( ( -LRB- cord-329553-93tbgn2b 306 20 in in IN cord-329553-93tbgn2b 306 21 S10 S10 NNP cord-329553-93tbgn2b 306 22 - - HYPH cord-329553-93tbgn2b 306 23 33D 33d NN cord-329553-93tbgn2b 306 24 ) ) -RRB- cord-329553-93tbgn2b 306 25 could could MD cord-329553-93tbgn2b 306 26 confer confer VB cord-329553-93tbgn2b 306 27 Golgi Golgi NNP cord-329553-93tbgn2b 306 28 localization localization NN cord-329553-93tbgn2b 306 29 , , , cord-329553-93tbgn2b 306 30 while while IN cord-329553-93tbgn2b 306 31 the the DT cord-329553-93tbgn2b 306 32 cytoplasmic cytoplasmic JJ cord-329553-93tbgn2b 306 33 domain domain NN cord-329553-93tbgn2b 306 34 alone alone RB cord-329553-93tbgn2b 306 35 ( ( -LRB- cord-329553-93tbgn2b 306 36 in in IN cord-329553-93tbgn2b 306 37 S9D s9d NN cord-329553-93tbgn2b 306 38 ) ) -RRB- cord-329553-93tbgn2b 306 39 or or CC cord-329553-93tbgn2b 306 40 the the DT cord-329553-93tbgn2b 306 41 COOH COOH NNP cord-329553-93tbgn2b 306 42 - - HYPH cord-329553-93tbgn2b 306 43 terminal terminal JJ cord-329553-93tbgn2b 306 44 flanking flanking NN cord-329553-93tbgn2b 306 45 sequence sequence NN cord-329553-93tbgn2b 306 46 alone alone RB cord-329553-93tbgn2b 306 47 ( ( -LRB- cord-329553-93tbgn2b 306 48 in in IN cord-329553-93tbgn2b 306 49 S27 S27 NNP cord-329553-93tbgn2b 306 50 - - HYPH cord-329553-93tbgn2b 306 51 33D 33D NNP cord-329553-93tbgn2b 306 52 ) ) -RRB- cord-329553-93tbgn2b 306 53 was be VBD cord-329553-93tbgn2b 306 54 not not RB cord-329553-93tbgn2b 306 55 capable capable JJ cord-329553-93tbgn2b 306 56 of of IN cord-329553-93tbgn2b 306 57 conferring confer VBG cord-329553-93tbgn2b 306 58 Golgi Golgi NNP cord-329553-93tbgn2b 306 59 localization localization NN cord-329553-93tbgn2b 306 60 . . . cord-329553-93tbgn2b 307 1 These these DT cord-329553-93tbgn2b 307 2 results result NNS cord-329553-93tbgn2b 307 3 thus thus RB cord-329553-93tbgn2b 307 4 demonstrate demonstrate VBP cord-329553-93tbgn2b 307 5 that that IN cord-329553-93tbgn2b 307 6 the the DT cord-329553-93tbgn2b 307 7 17-residue 17-residue CD cord-329553-93tbgn2b 307 8 transmembrane transmembrane NN cord-329553-93tbgn2b 307 9 domain domain NN cord-329553-93tbgn2b 307 10 of of IN cord-329553-93tbgn2b 307 11 ST ST NNP cord-329553-93tbgn2b 307 12 is be VBZ cord-329553-93tbgn2b 307 13 sufficient sufficient JJ cord-329553-93tbgn2b 307 14 for for IN cord-329553-93tbgn2b 307 15 Golgi Golgi NNP cord-329553-93tbgn2b 307 16 localization localization NN cord-329553-93tbgn2b 307 17 . . . cord-329553-93tbgn2b 308 1 Further further JJ cord-329553-93tbgn2b 308 2 characterization characterization NN cord-329553-93tbgn2b 308 3 of of IN cord-329553-93tbgn2b 308 4 the the DT cord-329553-93tbgn2b 308 5 fusion fusion NN cord-329553-93tbgn2b 308 6 protein protein NN cord-329553-93tbgn2b 308 7 S10 S10 NNP cord-329553-93tbgn2b 308 8 - - HYPH cord-329553-93tbgn2b 308 9 26D 26d NN cord-329553-93tbgn2b 308 10 by by IN cord-329553-93tbgn2b 308 11 lectin lectin NN cord-329553-93tbgn2b 308 12 affinity affinity NN cord-329553-93tbgn2b 308 13 chromatography chromatography NN cord-329553-93tbgn2b 308 14 demonstrated demonstrate VBD cord-329553-93tbgn2b 308 15 thatthe thatthe DT cord-329553-93tbgn2b 308 16 17-residue 17-residue CD cord-329553-93tbgn2b 308 17 sequence sequence NN cord-329553-93tbgn2b 308 18 can can MD cord-329553-93tbgn2b 308 19 confer confer VB cord-329553-93tbgn2b 308 20 the the DT cord-329553-93tbgn2b 308 21 same same JJ cord-329553-93tbgn2b 308 22 Golgi Golgi NNP cord-329553-93tbgn2b 308 23 subcompartment subcompartment NN cord-329553-93tbgn2b 308 24 localization localization NN cord-329553-93tbgn2b 308 25 as as IN cord-329553-93tbgn2b 308 26 the the DT cord-329553-93tbgn2b 308 27 NHZ NHZ NNP cord-329553-93tbgn2b 308 28 - - HYPH cord-329553-93tbgn2b 308 29 terminal terminal NN cord-329553-93tbgn2b 308 30 33-and 33-and CD cord-329553-93tbgn2b 308 31 64-residue 64-residue CD cord-329553-93tbgn2b 308 32 sequence sequence NN cord-329553-93tbgn2b 308 33 of of IN cord-329553-93tbgn2b 308 34 ST ST NNP cord-329553-93tbgn2b 308 35 . . . cord-329553-93tbgn2b 309 1 Recently recently RB cord-329553-93tbgn2b 309 2 , , , cord-329553-93tbgn2b 309 3 a a DT cord-329553-93tbgn2b 309 4 common common JJ cord-329553-93tbgn2b 309 5 peptide peptide NN cord-329553-93tbgn2b 309 6 stretch stretch NN cord-329553-93tbgn2b 309 7 was be VBD cord-329553-93tbgn2b 309 8 described describe VBN cord-329553-93tbgn2b 309 9 for for IN cord-329553-93tbgn2b 309 10 Golgi golgi NN cord-329553-93tbgn2b 309 11 - - HYPH cord-329553-93tbgn2b 309 12 localized localize VBN cord-329553-93tbgn2b 309 13 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 309 14 ( ( -LRB- cord-329553-93tbgn2b 309 15 Bendiak Bendiak NNP cord-329553-93tbgn2b 309 16 , , , cord-329553-93tbgn2b 309 17 1990 1990 CD cord-329553-93tbgn2b 309 18 ) ) -RRB- cord-329553-93tbgn2b 309 19 . . . cord-329553-93tbgn2b 310 1 This this DT cord-329553-93tbgn2b 310 2 stretch stretch NN cord-329553-93tbgn2b 310 3 ( ( -LRB- cord-329553-93tbgn2b 310 4 Ser Ser NNP cord-329553-93tbgn2b 310 5 - - HYPH cord-329553-93tbgn2b 310 6 Gln Gln NNP cord-329553-93tbgn2b 310 7 - - HYPH cord-329553-93tbgn2b 310 8 Glu Glu NNP cord-329553-93tbgn2b 310 9 - - HYPH cord-329553-93tbgn2b 310 10 Lys Lys NNP cord-329553-93tbgn2b 310 11 ) ) -RRB- cord-329553-93tbgn2b 310 12 is be VBZ cord-329553-93tbgn2b 310 13 located locate VBN cord-329553-93tbgn2b 310 14 between between IN cord-329553-93tbgn2b 310 15 residues residue NNS cord-329553-93tbgn2b 310 16 46 46 CD cord-329553-93tbgn2b 310 17 and and CC cord-329553-93tbgn2b 310 18 51 51 CD cord-329553-93tbgn2b 310 19 ( ( -LRB- cord-329553-93tbgn2b 310 20 residue residue NN cord-329553-93tbgn2b 310 21 47 47 CD cord-329553-93tbgn2b 310 22 - - SYM cord-329553-93tbgn2b 310 23 50 50 CD cord-329553-93tbgn2b 310 24 ) ) -RRB- cord-329553-93tbgn2b 310 25 in in IN cord-329553-93tbgn2b 310 26 ST ST NNP cord-329553-93tbgn2b 310 27 . . . cord-329553-93tbgn2b 311 1 Our -PRON- PRP$ cord-329553-93tbgn2b 311 2 results result NNS cord-329553-93tbgn2b 311 3 clearly clearly RB cord-329553-93tbgn2b 311 4 demonstrate demonstrate VBP cord-329553-93tbgn2b 311 5 that that IN cord-329553-93tbgn2b 311 6 this this DT cord-329553-93tbgn2b 311 7 stretch stretch NN cord-329553-93tbgn2b 311 8 is be VBZ cord-329553-93tbgn2b 311 9 not not RB cord-329553-93tbgn2b 311 10 critical critical JJ cord-329553-93tbgn2b 311 11 for for IN cord-329553-93tbgn2b 311 12 Golgi Golgi NNP cord-329553-93tbgn2b 311 13 localization localization NN cord-329553-93tbgn2b 311 14 . . . cord-329553-93tbgn2b 312 1 Furthermore furthermore RB cord-329553-93tbgn2b 312 2 , , , cord-329553-93tbgn2b 312 3 the the DT cord-329553-93tbgn2b 312 4 sequence sequence NN cord-329553-93tbgn2b 312 5 of of IN cord-329553-93tbgn2b 312 6 this this DT cord-329553-93tbgn2b 312 7 stretch stretch NN cord-329553-93tbgn2b 312 8 is be VBZ cord-329553-93tbgn2b 312 9 not not RB cord-329553-93tbgn2b 312 10 conserved conserve VBN cord-329553-93tbgn2b 312 11 in in IN cord-329553-93tbgn2b 312 12 the the DT cord-329553-93tbgn2b 312 13 human human JJ cord-329553-93tbgn2b 312 14 ST ST NNP cord-329553-93tbgn2b 312 15 ( ( -LRB- cord-329553-93tbgn2b 312 16 Ser Ser NNP cord-329553-93tbgn2b 312 17 - - HYPH cord-329553-93tbgn2b 312 18 Leu Leu NNP cord-329553-93tbgn2b 312 19 - - HYPH cord-329553-93tbgn2b 312 20 Gly Gly NNP cord-329553-93tbgn2b 312 21 - - HYPH cord-329553-93tbgn2b 312 22 Lys Lys NNP cord-329553-93tbgn2b 312 23 ) ) -RRB- cord-329553-93tbgn2b 313 1 ( ( -LRB- cord-329553-93tbgn2b 313 2 Grundmann Grundmann NNP cord-329553-93tbgn2b 313 3 et et NNP cord-329553-93tbgn2b 313 4 al al NNP cord-329553-93tbgn2b 313 5 . . NNP cord-329553-93tbgn2b 313 6 , , , cord-329553-93tbgn2b 313 7 1990 1990 CD cord-329553-93tbgn2b 313 8 ) ) -RRB- cord-329553-93tbgn2b 313 9 . . . cord-329553-93tbgn2b 314 1 Since since IN cord-329553-93tbgn2b 314 2 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 314 3 are be VBP cord-329553-93tbgn2b 314 4 localized localize VBN cord-329553-93tbgn2b 314 5 to to IN cord-329553-93tbgn2b 314 6 distinct distinct JJ cord-329553-93tbgn2b 314 7 subcompartments subcompartment NNS cord-329553-93tbgn2b 314 8 of of IN cord-329553-93tbgn2b 314 9 the the DT cord-329553-93tbgn2b 314 10 Golgi Golgi NNP cord-329553-93tbgn2b 314 11 apparatus apparatus NN cord-329553-93tbgn2b 314 12 , , , cord-329553-93tbgn2b 314 13 it -PRON- PRP cord-329553-93tbgn2b 314 14 is be VBZ cord-329553-93tbgn2b 314 15 hard hard JJ cord-329553-93tbgn2b 314 16 to to TO cord-329553-93tbgn2b 314 17 envision envision VB cord-329553-93tbgn2b 314 18 a a DT cord-329553-93tbgn2b 314 19 common common JJ cord-329553-93tbgn2b 314 20 sequence sequence NN cord-329553-93tbgn2b 314 21 for for IN cord-329553-93tbgn2b 314 22 distinct distinct JJ cord-329553-93tbgn2b 314 23 subcompartment subcompartment JJ cord-329553-93tbgn2b 314 24 localization localization NN cord-329553-93tbgn2b 314 25 . . . cord-329553-93tbgn2b 315 1 Further further JJ cord-329553-93tbgn2b 315 2 studies study NNS cord-329553-93tbgn2b 315 3 with with IN cord-329553-93tbgn2b 315 4 other other JJ cord-329553-93tbgn2b 315 5 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 315 6 will will MD cord-329553-93tbgn2b 315 7 reveal reveal VB cord-329553-93tbgn2b 315 8 the the DT cord-329553-93tbgn2b 315 9 structural structural JJ cord-329553-93tbgn2b 315 10 features feature NNS cord-329553-93tbgn2b 315 11 required require VBN cord-329553-93tbgn2b 315 12 for for IN cord-329553-93tbgn2b 315 13 localization localization NN cord-329553-93tbgn2b 315 14 to to IN cord-329553-93tbgn2b 315 15 other other JJ cord-329553-93tbgn2b 315 16 subcompartments subcompartment NNS cord-329553-93tbgn2b 315 17 of of IN cord-329553-93tbgn2b 315 18 the the DT cord-329553-93tbgn2b 315 19 Golgi Golgi NNP cord-329553-93tbgn2b 315 20 apparatus apparatus NN cord-329553-93tbgn2b 315 21 . . . cord-329553-93tbgn2b 316 1 Since since IN cord-329553-93tbgn2b 316 2 our -PRON- PRP$ cord-329553-93tbgn2b 316 3 study study NN cord-329553-93tbgn2b 316 4 involved involve VBD cord-329553-93tbgn2b 316 5 extensive extensive JJ cord-329553-93tbgn2b 316 6 " " `` cord-329553-93tbgn2b 316 7 Cut cut VB cord-329553-93tbgn2b 316 8 - - HYPH cord-329553-93tbgn2b 316 9 and and CC cord-329553-93tbgn2b 316 10 - - HYPH cord-329553-93tbgn2b 316 11 Paste paste NN cord-329553-93tbgn2b 316 12 ex ex NN cord-329553-93tbgn2b 316 13 - - NN cord-329553-93tbgn2b 316 14 periments periment NNS cord-329553-93tbgn2b 316 15 for for IN cord-329553-93tbgn2b 316 16 the the DT cord-329553-93tbgn2b 316 17 fusion fusion NN cord-329553-93tbgn2b 316 18 of of IN cord-329553-93tbgn2b 316 19 domains domain NNS cord-329553-93tbgn2b 316 20 derived derive VBN cord-329553-93tbgn2b 316 21 from from IN cord-329553-93tbgn2b 316 22 two two CD cord-329553-93tbgn2b 316 23 different different JJ cord-329553-93tbgn2b 316 24 proteins protein NNS cord-329553-93tbgn2b 316 25 , , , cord-329553-93tbgn2b 316 26 one one PRP cord-329553-93tbgn2b 316 27 may may MD cord-329553-93tbgn2b 316 28 argue argue VB cord-329553-93tbgn2b 316 29 that that IN cord-329553-93tbgn2b 316 30 conformational conformational JJ cord-329553-93tbgn2b 316 31 changes change NNS cord-329553-93tbgn2b 316 32 might may MD cord-329553-93tbgn2b 316 33 have have VB cord-329553-93tbgn2b 316 34 contributed contribute VBN cord-329553-93tbgn2b 316 35 to to IN cord-329553-93tbgn2b 316 36 the the DT cord-329553-93tbgn2b 316 37 results result NNS cord-329553-93tbgn2b 316 38 observed observe VBN cord-329553-93tbgn2b 316 39 in in IN cord-329553-93tbgn2b 316 40 our -PRON- PRP$ cord-329553-93tbgn2b 316 41 system system NN cord-329553-93tbgn2b 316 42 . . . cord-329553-93tbgn2b 317 1 This this DT cord-329553-93tbgn2b 317 2 seems seem VBZ cord-329553-93tbgn2b 317 3 highly highly RB cord-329553-93tbgn2b 317 4 unlikely unlikely JJ cord-329553-93tbgn2b 317 5 because because IN cord-329553-93tbgn2b 317 6 the the DT cord-329553-93tbgn2b 317 7 chimeric chimeric JJ cord-329553-93tbgn2b 317 8 proteins protein NNS cord-329553-93tbgn2b 317 9 were be VBD cord-329553-93tbgn2b 317 10 transported transport VBN cord-329553-93tbgn2b 317 11 from from IN cord-329553-93tbgn2b 317 12 the the DT cord-329553-93tbgn2b 317 13 ER ER NNP cord-329553-93tbgn2b 317 14 to to IN cord-329553-93tbgn2b 317 15 the the DT cord-329553-93tbgn2b 317 16 Golgi Golgi NNP cord-329553-93tbgn2b 317 17 apparatus apparatus NN cord-329553-93tbgn2b 317 18 as as RB cord-329553-93tbgn2b 317 19 efficiently efficiently RB cord-329553-93tbgn2b 317 20 as as IN cord-329553-93tbgn2b 317 21 normal normal JJ cord-329553-93tbgn2b 317 22 DPPIV DPPIV NNP cord-329553-93tbgn2b 317 23 and and CC cord-329553-93tbgn2b 317 24 were be VBD cord-329553-93tbgn2b 317 25 modified modify VBN cord-329553-93tbgn2b 317 26 by by IN cord-329553-93tbgn2b 317 27 enzymes enzyme NNS cord-329553-93tbgn2b 317 28 in in IN cord-329553-93tbgn2b 317 29 all all DT cord-329553-93tbgn2b 317 30 subcompartments subcompartment NNS cord-329553-93tbgn2b 317 31 of of IN cord-329553-93tbgn2b 317 32 the the DT cord-329553-93tbgn2b 317 33 Golgi Golgi NNP cord-329553-93tbgn2b 317 34 apparatus apparatus NN cord-329553-93tbgn2b 317 35 . . . cord-329553-93tbgn2b 318 1 If if IN cord-329553-93tbgn2b 318 2 there there EX cord-329553-93tbgn2b 318 3 was be VBD cord-329553-93tbgn2b 318 4 any any DT cord-329553-93tbgn2b 318 5 conformational conformational JJ cord-329553-93tbgn2b 318 6 abnormality abnormality NN cord-329553-93tbgn2b 318 7 , , , cord-329553-93tbgn2b 318 8 the the DT cord-329553-93tbgn2b 318 9 fusion fusion NN cord-329553-93tbgn2b 318 10 proteins protein NNS cord-329553-93tbgn2b 318 11 would would MD cord-329553-93tbgn2b 318 12 have have VB cord-329553-93tbgn2b 318 13 been be VBN cord-329553-93tbgn2b 318 14 retained retain VBN cord-329553-93tbgn2b 318 15 in in IN cord-329553-93tbgn2b 318 16 the the DT cord-329553-93tbgn2b 318 17 ER ER NNP cord-329553-93tbgn2b 318 18 and/or and/or CC cord-329553-93tbgn2b 318 19 pre pre JJ cord-329553-93tbgn2b 318 20 - - JJ cord-329553-93tbgn2b 318 21 Golgi golgi JJ cord-329553-93tbgn2b 318 22 compartments compartment NNS cord-329553-93tbgn2b 318 23 . . . cord-329553-93tbgn2b 319 1 Many many JJ cord-329553-93tbgn2b 319 2 misfolded misfolde VBN cord-329553-93tbgn2b 319 3 chimeric chimeric JJ cord-329553-93tbgn2b 319 4 proteins protein NNS cord-329553-93tbgn2b 319 5 have have VBP cord-329553-93tbgn2b 319 6 been be VBN cord-329553-93tbgn2b 319 7 described describe VBN cord-329553-93tbgn2b 319 8 and and CC cord-329553-93tbgn2b 319 9 all all DT cord-329553-93tbgn2b 319 10 failed fail VBD cord-329553-93tbgn2b 319 11 to to TO cord-329553-93tbgn2b 319 12 be be VB cord-329553-93tbgn2b 319 13 transported transport VBN cord-329553-93tbgn2b 319 14 beyond beyond IN cord-329553-93tbgn2b 319 15 the the DT cord-329553-93tbgn2b 319 16 cis cis JJ cord-329553-93tbgn2b 319 17 - - HYPH cord-329553-93tbgn2b 319 18 face face NN cord-329553-93tbgn2b 319 19 of of IN cord-329553-93tbgn2b 319 20 the the DT cord-329553-93tbgn2b 319 21 Golgi Golgi NNP cord-329553-93tbgn2b 319 22 apparatus apparatus NN cord-329553-93tbgn2b 319 23 ( ( -LRB- cord-329553-93tbgn2b 319 24 Rose Rose NNP cord-329553-93tbgn2b 319 25 and and CC cord-329553-93tbgn2b 319 26 Doms Doms NNP cord-329553-93tbgn2b 319 27 , , , cord-329553-93tbgn2b 319 28 1988 1988 CD cord-329553-93tbgn2b 319 29 ) ) -RRB- cord-329553-93tbgn2b 319 30 . . . cord-329553-93tbgn2b 320 1 Furthermore furthermore RB cord-329553-93tbgn2b 320 2 , , , cord-329553-93tbgn2b 320 3 preliminary preliminary JJ cord-329553-93tbgn2b 320 4 experiments experiment NNS cord-329553-93tbgn2b 320 5 have have VBP cord-329553-93tbgn2b 320 6 shown show VBN cord-329553-93tbgn2b 320 7 that that IN cord-329553-93tbgn2b 320 8 all all PDT cord-329553-93tbgn2b 320 9 the the DT cord-329553-93tbgn2b 320 10 fusion fusion NN cord-329553-93tbgn2b 320 11 proteins protein NNS cord-329553-93tbgn2b 320 12 possessed possess VBD cord-329553-93tbgn2b 320 13 similar similar JJ cord-329553-93tbgn2b 320 14 enzymatic enzymatic JJ cord-329553-93tbgn2b 320 15 activity activity NN cord-329553-93tbgn2b 320 16 as as IN cord-329553-93tbgn2b 320 17 the the DT cord-329553-93tbgn2b 320 18 normal normal JJ cord-329553-93tbgn2b 320 19 DPPIV DPPIV NNP cord-329553-93tbgn2b 320 20 , , , cord-329553-93tbgn2b 320 21 suggesting suggest VBG cord-329553-93tbgn2b 320 22 no no DT cord-329553-93tbgn2b 320 23 global global JJ cord-329553-93tbgn2b 320 24 conformational conformational JJ cord-329553-93tbgn2b 320 25 change change NN cord-329553-93tbgn2b 320 26 in in IN cord-329553-93tbgn2b 320 27 the the DT cord-329553-93tbgn2b 320 28 ectodomain ectodomain NN cord-329553-93tbgn2b 320 29 of of IN cord-329553-93tbgn2b 320 30 DPPIV DPPIV NNP cord-329553-93tbgn2b 320 31 in in IN cord-329553-93tbgn2b 320 32 the the DT cord-329553-93tbgn2b 320 33 chimeric chimeric JJ cord-329553-93tbgn2b 320 34 proteins protein NNS cord-329553-93tbgn2b 320 35 . . . cord-329553-93tbgn2b 321 1 Additional additional JJ cord-329553-93tbgn2b 321 2 experiments experiment NNS cord-329553-93tbgn2b 321 3 demonstrated demonstrate VBD cord-329553-93tbgn2b 321 4 that that IN cord-329553-93tbgn2b 321 5 the the DT cord-329553-93tbgn2b 321 6 Golgi Golgi NNP cord-329553-93tbgn2b 321 7 localization localization NN cord-329553-93tbgn2b 321 8 of of IN cord-329553-93tbgn2b 321 9 the the DT cord-329553-93tbgn2b 321 10 chimeric chimeric JJ cord-329553-93tbgn2b 321 11 proteins protein NNS cord-329553-93tbgn2b 321 12 by by IN cord-329553-93tbgn2b 321 13 the the DT cord-329553-93tbgn2b 321 14 ST ST NNP cord-329553-93tbgn2b 321 15 transmembrane transmembrane NNP cord-329553-93tbgn2b 321 16 domain domain NN cord-329553-93tbgn2b 321 17 is be VBZ cord-329553-93tbgn2b 321 18 a a DT cord-329553-93tbgn2b 321 19 specific specific JJ cord-329553-93tbgn2b 321 20 event event NN cord-329553-93tbgn2b 321 21 , , , cord-329553-93tbgn2b 321 22 because because IN cord-329553-93tbgn2b 321 23 the the DT cord-329553-93tbgn2b 321 24 replacementof replacementof NNP cord-329553-93tbgn2b 321 25 the the DT cord-329553-93tbgn2b 321 26 transmembrane transmembrane NN cord-329553-93tbgn2b 321 27 domain domain NN cord-329553-93tbgn2b 321 28 of of IN cord-329553-93tbgn2b 321 29 DPPIV DPPIV NNP cord-329553-93tbgn2b 321 30 by by IN cord-329553-93tbgn2b 321 31 that that DT cord-329553-93tbgn2b 321 32 of of IN cord-329553-93tbgn2b 321 33 another another DT cord-329553-93tbgn2b 321 34 type type NN cord-329553-93tbgn2b 321 35 II II NNP cord-329553-93tbgn2b 321 36 surface surface NN cord-329553-93tbgn2b 321 37 protein protein NN cord-329553-93tbgn2b 321 38 resulted result VBD cord-329553-93tbgn2b 321 39 in in IN cord-329553-93tbgn2b 321 40 a a DT cord-329553-93tbgn2b 321 41 chimeric chimeric JJ cord-329553-93tbgn2b 321 42 protein protein NN cord-329553-93tbgn2b 321 43 that that WDT cord-329553-93tbgn2b 321 44 was be VBD cord-329553-93tbgn2b 321 45 transported transport VBN cord-329553-93tbgn2b 321 46 to to IN cord-329553-93tbgn2b 321 47 the the DT cord-329553-93tbgn2b 321 48 cell cell NN cord-329553-93tbgn2b 321 49 surface surface NN cord-329553-93tbgn2b 321 50 ( ( -LRB- cord-329553-93tbgn2b 321 51 data datum NNS cord-329553-93tbgn2b 321 52 not not RB cord-329553-93tbgn2b 321 53 shown show VBN cord-329553-93tbgn2b 321 54 ) ) -RRB- cord-329553-93tbgn2b 321 55 . . . cord-329553-93tbgn2b 322 1 The the DT cord-329553-93tbgn2b 322 2 Golgi Golgi NNP cord-329553-93tbgn2b 322 3 localization localization NN cord-329553-93tbgn2b 322 4 of of IN cord-329553-93tbgn2b 322 5 the the DT cord-329553-93tbgn2b 322 6 fusion fusion NN cord-329553-93tbgn2b 322 7 proteins protein NNS cord-329553-93tbgn2b 322 8 could could MD cord-329553-93tbgn2b 322 9 be be VB cord-329553-93tbgn2b 322 10 mediated mediate VBN cord-329553-93tbgn2b 322 11 by by IN cord-329553-93tbgn2b 322 12 their -PRON- PRP$ cord-329553-93tbgn2b 322 13 specific specific JJ cord-329553-93tbgn2b 322 14 retention retention NN cord-329553-93tbgn2b 322 15 in in IN cord-329553-93tbgn2b 322 16 the the DT cord-329553-93tbgn2b 322 17 Golgi Golgi NNP cord-329553-93tbgn2b 322 18 subcompartment subcompartment NN cord-329553-93tbgn2b 322 19 ( ( -LRB- cord-329553-93tbgn2b 322 20 in in IN cord-329553-93tbgn2b 322 21 this this DT cord-329553-93tbgn2b 322 22 case case NN cord-329553-93tbgn2b 322 23 , , , cord-329553-93tbgn2b 322 24 the the DT cord-329553-93tbgn2b 322 25 Golgi Golgi NNP cord-329553-93tbgn2b 322 26 localization localization NN cord-329553-93tbgn2b 322 27 signal signal NN cord-329553-93tbgn2b 322 28 functions function NNS cord-329553-93tbgn2b 322 29 as as IN cord-329553-93tbgn2b 322 30 a a DT cord-329553-93tbgn2b 322 31 retention retention NN cord-329553-93tbgn2b 322 32 signal signal NN cord-329553-93tbgn2b 322 33 ) ) -RRB- cord-329553-93tbgn2b 322 34 . . . cord-329553-93tbgn2b 323 1 Alternatively alternatively RB cord-329553-93tbgn2b 323 2 , , , cord-329553-93tbgn2b 323 3 they -PRON- PRP cord-329553-93tbgn2b 323 4 could could MD cord-329553-93tbgn2b 323 5 be be VB cord-329553-93tbgn2b 323 6 initially initially RB cord-329553-93tbgn2b 323 7 transported transport VBN cord-329553-93tbgn2b 323 8 to to IN cord-329553-93tbgn2b 323 9 the the DT cord-329553-93tbgn2b 323 10 cell cell NN cord-329553-93tbgn2b 323 11 surface surface NN cord-329553-93tbgn2b 323 12 followed follow VBN cord-329553-93tbgn2b 323 13 by by IN cord-329553-93tbgn2b 323 14 their -PRON- PRP$ cord-329553-93tbgn2b 323 15 efficient efficient JJ cord-329553-93tbgn2b 323 16 retrieval retrieval NN cord-329553-93tbgn2b 323 17 and and CC cord-329553-93tbgn2b 323 18 transcytosis transcytosis NNP cord-329553-93tbgn2b 323 19 back back RB cord-329553-93tbgn2b 323 20 to to IN cord-329553-93tbgn2b 323 21 the the DT cord-329553-93tbgn2b 323 22 final final JJ cord-329553-93tbgn2b 323 23 Golgi Golgi NNP cord-329553-93tbgn2b 323 24 localization localization NN cord-329553-93tbgn2b 323 25 . . . cord-329553-93tbgn2b 324 1 To to TO cord-329553-93tbgn2b 324 2 distinguish distinguish VB cord-329553-93tbgn2b 324 3 between between IN cord-329553-93tbgn2b 324 4 these these DT cord-329553-93tbgn2b 324 5 two two CD cord-329553-93tbgn2b 324 6 possibilities possibility NNS cord-329553-93tbgn2b 324 7 , , , cord-329553-93tbgn2b 324 8 we -PRON- PRP cord-329553-93tbgn2b 324 9 used use VBD cord-329553-93tbgn2b 324 10 the the DT cord-329553-93tbgn2b 324 11 membrane membrane NN cord-329553-93tbgn2b 324 12 - - HYPH cord-329553-93tbgn2b 324 13 impermeable impermeable JJ cord-329553-93tbgn2b 324 14 reagent reagent NN cord-329553-93tbgn2b 324 15 NHS NHS NNP cord-329553-93tbgn2b 324 16 - - HYPH cord-329553-93tbgn2b 324 17 biotin biotin NN cord-329553-93tbgn2b 324 18 to to TO cord-329553-93tbgn2b 324 19 selectively selectively RB cord-329553-93tbgn2b 324 20 tag tag VB cord-329553-93tbgn2b 324 21 the the DT cord-329553-93tbgn2b 324 22 proteins protein NNS cord-329553-93tbgn2b 324 23 present present JJ cord-329553-93tbgn2b 324 24 on on IN cord-329553-93tbgn2b 324 25 the the DT cord-329553-93tbgn2b 324 26 cell cell NN cord-329553-93tbgn2b 324 27 surface surface NN cord-329553-93tbgn2b 324 28 after after IN cord-329553-93tbgn2b 324 29 a a DT cord-329553-93tbgn2b 324 30 single single JJ cord-329553-93tbgn2b 324 31 pulse pulse NN cord-329553-93tbgn2b 324 32 and and CC cord-329553-93tbgn2b 324 33 several several JJ cord-329553-93tbgn2b 324 34 chase chase NN cord-329553-93tbgn2b 324 35 intervals interval NNS cord-329553-93tbgn2b 324 36 . . . cord-329553-93tbgn2b 325 1 The the DT cord-329553-93tbgn2b 325 2 biotinylated biotinylated JJ cord-329553-93tbgn2b 325 3 proteins protein NNS cord-329553-93tbgn2b 325 4 were be VBD cord-329553-93tbgn2b 325 5 specifically specifically RB cord-329553-93tbgn2b 325 6 recovered recover VBN cord-329553-93tbgn2b 325 7 by by IN cord-329553-93tbgn2b 325 8 absorption absorption NN cord-329553-93tbgn2b 325 9 to to IN cord-329553-93tbgn2b 325 10 streptavidin streptavidin NN cord-329553-93tbgn2b 325 11 - - HYPH cord-329553-93tbgn2b 325 12 agarose agarose VBN cord-329553-93tbgn2b 325 13 and and CC cord-329553-93tbgn2b 325 14 served serve VBD cord-329553-93tbgn2b 325 15 as as IN cord-329553-93tbgn2b 325 16 a a DT cord-329553-93tbgn2b 325 17 measure measure NN cord-329553-93tbgn2b 325 18 of of IN cord-329553-93tbgn2b 325 19 surface surface NN cord-329553-93tbgn2b 325 20 expression expression NN cord-329553-93tbgn2b 325 21 . . . cord-329553-93tbgn2b 326 1 Efficient efficient JJ cord-329553-93tbgn2b 326 2 surface surface NN cord-329553-93tbgn2b 326 3 expression expression NN cord-329553-93tbgn2b 326 4 of of IN cord-329553-93tbgn2b 326 5 DPPIV DPPIV NNP cord-329553-93tbgn2b 326 6 was be VBD cord-329553-93tbgn2b 326 7 detected detect VBN cord-329553-93tbgn2b 326 8 . . . cord-329553-93tbgn2b 327 1 Studies study NNS cord-329553-93tbgn2b 327 2 with with IN cord-329553-93tbgn2b 327 3 S33D s33d JJ cord-329553-93tbgn2b 327 4 and and CC cord-329553-93tbgn2b 327 5 S10 S10 NNP cord-329553-93tbgn2b 327 6 - - HYPH cord-329553-93tbgn2b 327 7 26D 26D NNP cord-329553-93tbgn2b 327 8 demonstrated demonstrate VBD cord-329553-93tbgn2b 327 9 that that IN cord-329553-93tbgn2b 327 10 they -PRON- PRP cord-329553-93tbgn2b 327 11 were be VBD cord-329553-93tbgn2b 327 12 hardly hardly RB cord-329553-93tbgn2b 327 13 detectable detectable JJ cord-329553-93tbgn2b 327 14 on on IN cord-329553-93tbgn2b 327 15 the the DT cord-329553-93tbgn2b 327 16 cell cell NN cord-329553-93tbgn2b 327 17 surface surface NN cord-329553-93tbgn2b 327 18 . . . cord-329553-93tbgn2b 328 1 These these DT cord-329553-93tbgn2b 328 2 results result NNS cord-329553-93tbgn2b 328 3 established establish VBD cord-329553-93tbgn2b 328 4 that that IN cord-329553-93tbgn2b 328 5 the the DT cord-329553-93tbgn2b 328 6 majority majority NN cord-329553-93tbgn2b 328 7 of of IN cord-329553-93tbgn2b 328 8 S33D s33d JJ cord-329553-93tbgn2b 328 9 and and CC cord-329553-93tbgn2b 328 10 S10 S10 NNP cord-329553-93tbgn2b 328 11 - - HYPH cord-329553-93tbgn2b 328 12 26D 26d NN cord-329553-93tbgn2b 328 13 were be VBD cord-329553-93tbgn2b 328 14 specifically specifically RB cord-329553-93tbgn2b 328 15 retained retain VBN cord-329553-93tbgn2b 328 16 in in IN cord-329553-93tbgn2b 328 17 the the DT cord-329553-93tbgn2b 328 18 Golgi Golgi NNP cord-329553-93tbgn2b 328 19 apparatus apparatus NN cord-329553-93tbgn2b 328 20 and and CC cord-329553-93tbgn2b 328 21 that that IN cord-329553-93tbgn2b 328 22 the the DT cord-329553-93tbgn2b 328 23 17-residue 17-residue CD cord-329553-93tbgn2b 328 24 transmembrane transmembrane NN cord-329553-93tbgn2b 328 25 domain domain NN cord-329553-93tbgn2b 328 26 of of IN cord-329553-93tbgn2b 328 27 ST ST NNP cord-329553-93tbgn2b 328 28 functions function NNS cord-329553-93tbgn2b 328 29 as as IN cord-329553-93tbgn2b 328 30 a a DT cord-329553-93tbgn2b 328 31 retention retention NN cord-329553-93tbgn2b 328 32 signal signal NN cord-329553-93tbgn2b 328 33 for for IN cord-329553-93tbgn2b 328 34 the the DT cord-329553-93tbgn2b 328 35 Golgi Golgi NNP cord-329553-93tbgn2b 328 36 subcompartment subcompartment NN cord-329553-93tbgn2b 328 37 . . . cord-329553-93tbgn2b 329 1 Our -PRON- PRP$ cord-329553-93tbgn2b 329 2 studies study NNS cord-329553-93tbgn2b 329 3 demonstrated demonstrate VBD cord-329553-93tbgn2b 329 4 that that IN cord-329553-93tbgn2b 329 5 the the DT cord-329553-93tbgn2b 329 6 membrane membrane NN cord-329553-93tbgn2b 329 7 - - HYPH cord-329553-93tbgn2b 329 8 spanning span VBG cord-329553-93tbgn2b 329 9 region region NN cord-329553-93tbgn2b 329 10 of of IN cord-329553-93tbgn2b 329 11 ST ST NNP cord-329553-93tbgn2b 329 12 , , , cord-329553-93tbgn2b 329 13 in in IN cord-329553-93tbgn2b 329 14 addition addition NN cord-329553-93tbgn2b 329 15 to to IN cord-329553-93tbgn2b 329 16 its -PRON- PRP$ cord-329553-93tbgn2b 329 17 passive passive JJ cord-329553-93tbgn2b 329 18 role role NN cord-329553-93tbgn2b 329 19 in in IN cord-329553-93tbgn2b 329 20 membrane membrane NNP cord-329553-93tbgn2b 329 21 anchorage anchorage NNP cord-329553-93tbgn2b 329 22 , , , cord-329553-93tbgn2b 329 23 plays play VBZ cord-329553-93tbgn2b 329 24 a a DT cord-329553-93tbgn2b 329 25 dominant dominant JJ cord-329553-93tbgn2b 329 26 role role NN cord-329553-93tbgn2b 329 27 in in IN cord-329553-93tbgn2b 329 28 Golgi Golgi NNP cord-329553-93tbgn2b 329 29 retention retention NN cord-329553-93tbgn2b 329 30 . . . cord-329553-93tbgn2b 330 1 Our -PRON- PRP$ cord-329553-93tbgn2b 330 2 observations observation NNS cord-329553-93tbgn2b 330 3 raised raise VBD cord-329553-93tbgn2b 330 4 the the DT cord-329553-93tbgn2b 330 5 question question NN cord-329553-93tbgn2b 330 6 of of IN cord-329553-93tbgn2b 330 7 whether whether IN cord-329553-93tbgn2b 330 8 the the DT cord-329553-93tbgn2b 330 9 transmembrane transmembrane NN cord-329553-93tbgn2b 330 10 domains domain NNS cord-329553-93tbgn2b 330 11 of of IN cord-329553-93tbgn2b 330 12 other other JJ cord-329553-93tbgn2b 330 13 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 330 14 play play VBP cord-329553-93tbgn2b 330 15 similar similar JJ cord-329553-93tbgn2b 330 16 roles role NNS cord-329553-93tbgn2b 330 17 in in IN cord-329553-93tbgn2b 330 18 specifying specify VBG cord-329553-93tbgn2b 330 19 their -PRON- PRP$ cord-329553-93tbgn2b 330 20 subcompartment subcompartment JJ cord-329553-93tbgn2b 330 21 Golgi Golgi NNP cord-329553-93tbgn2b 330 22 localization localization NN cord-329553-93tbgn2b 330 23 . . . cord-329553-93tbgn2b 331 1 Comparison comparison NN cord-329553-93tbgn2b 331 2 of of IN cord-329553-93tbgn2b 331 3 the the DT cord-329553-93tbgn2b 331 4 primary primary JJ cord-329553-93tbgn2b 331 5 sequences sequence NNS cord-329553-93tbgn2b 331 6 of of IN cord-329553-93tbgn2b 331 7 GT GT NNP cord-329553-93tbgn2b 331 8 revealed reveal VBD cord-329553-93tbgn2b 331 9 that that IN cord-329553-93tbgn2b 331 10 the the DT cord-329553-93tbgn2b 331 11 entire entire JJ cord-329553-93tbgn2b 331 12 transmembrane transmembrane NN cord-329553-93tbgn2b 331 13 domain domain NN cord-329553-93tbgn2b 331 14 is be VBZ cord-329553-93tbgn2b 331 15 identical identical JJ cord-329553-93tbgn2b 331 16 in in IN cord-329553-93tbgn2b 331 17 human human JJ cord-329553-93tbgn2b 331 18 and and CC cord-329553-93tbgn2b 331 19 bovine bovine JJ cord-329553-93tbgn2b 331 20 and and CC cord-329553-93tbgn2b 331 21 that that IN cord-329553-93tbgn2b 331 22 19 19 CD cord-329553-93tbgn2b 331 23 of of IN cord-329553-93tbgn2b 331 24 the the DT cord-329553-93tbgn2b 331 25 20 20 CD cord-329553-93tbgn2b 331 26 residues residue NNS cord-329553-93tbgn2b 331 27 in in IN cord-329553-93tbgn2b 331 28 the the DT cord-329553-93tbgn2b 331 29 transmembrane transmembrane NN cord-329553-93tbgn2b 331 30 domain domain NN cord-329553-93tbgn2b 331 31 are be VBP cord-329553-93tbgn2b 331 32 also also RB cord-329553-93tbgn2b 331 33 conserved conserve VBN cord-329553-93tbgn2b 331 34 in in IN cord-329553-93tbgn2b 331 35 mouse mouse NN cord-329553-93tbgn2b 331 36 ( ( -LRB- cord-329553-93tbgn2b 331 37 DAgostaro DAgostaro NNP cord-329553-93tbgn2b 331 38 et et NNP cord-329553-93tbgn2b 331 39 al al NNP cord-329553-93tbgn2b 331 40 . . NNP cord-329553-93tbgn2b 331 41 , , , cord-329553-93tbgn2b 331 42 1989 1989 CD cord-329553-93tbgn2b 331 43 ; ; : cord-329553-93tbgn2b 332 1 Masri Masri NNP cord-329553-93tbgn2b 332 2 et et NNP cord-329553-93tbgn2b 332 3 al al NNP cord-329553-93tbgn2b 332 4 . . . cord-329553-93tbgn2b 333 1 , , , cord-329553-93tbgn2b 333 2 1988 1988 CD cord-329553-93tbgn2b 333 3 ; ; : cord-329553-93tbgn2b 333 4 Russo Russo NNP cord-329553-93tbgn2b 333 5 et et FW cord-329553-93tbgn2b 333 6 al al NNP cord-329553-93tbgn2b 333 7 . . NNP cord-329553-93tbgn2b 333 8 , , , cord-329553-93tbgn2b 333 9 1990 1990 CD cord-329553-93tbgn2b 334 1 ; ; : cord-329553-93tbgn2b 334 2 Shaper Shaper NNP cord-329553-93tbgn2b 334 3 et et FW cord-329553-93tbgn2b 334 4 al al NNP cord-329553-93tbgn2b 334 5 . . NNP cord-329553-93tbgn2b 334 6 , , , cord-329553-93tbgn2b 334 7 1988 1988 CD cord-329553-93tbgn2b 334 8 ) ) -RRB- cord-329553-93tbgn2b 334 9 . . . cord-329553-93tbgn2b 335 1 Furthermore furthermore RB cord-329553-93tbgn2b 335 2 , , , cord-329553-93tbgn2b 335 3 the the DT cord-329553-93tbgn2b 335 4 sequence sequence NN cord-329553-93tbgn2b 335 5 of of IN cord-329553-93tbgn2b 335 6 the the DT cord-329553-93tbgn2b 335 7 transmembrane transmembrane NN cord-329553-93tbgn2b 335 8 domain domain NN cord-329553-93tbgn2b 335 9 of of IN cord-329553-93tbgn2b 335 10 NTI NTI NNP cord-329553-93tbgn2b 335 11 is be VBZ cord-329553-93tbgn2b 335 12 totally totally RB cord-329553-93tbgn2b 335 13 conserved conserve VBN cord-329553-93tbgn2b 335 14 between between IN cord-329553-93tbgn2b 335 15 human human NN cord-329553-93tbgn2b 335 16 and and CC cord-329553-93tbgn2b 335 17 rabbit rabbit NN cord-329553-93tbgn2b 335 18 ( ( -LRB- cord-329553-93tbgn2b 335 19 Kumar Kumar NNP cord-329553-93tbgn2b 335 20 et et NNP cord-329553-93tbgn2b 335 21 al al NNP cord-329553-93tbgn2b 335 22 . . NNP cord-329553-93tbgn2b 335 23 , , , cord-329553-93tbgn2b 335 24 1990 1990 CD cord-329553-93tbgn2b 335 25 ; ; : cord-329553-93tbgn2b 335 26 Sarkar Sarkar NNP cord-329553-93tbgn2b 335 27 et et FW cord-329553-93tbgn2b 335 28 al al NNP cord-329553-93tbgn2b 335 29 . . NNP cord-329553-93tbgn2b 335 30 , , , cord-329553-93tbgn2b 335 31 1991 1991 CD cord-329553-93tbgn2b 335 32 ) ) -RRB- cord-329553-93tbgn2b 335 33 . . . cord-329553-93tbgn2b 336 1 The the DT cord-329553-93tbgn2b 336 2 high high JJ cord-329553-93tbgn2b 336 3 degree degree NN cord-329553-93tbgn2b 336 4 of of IN cord-329553-93tbgn2b 336 5 conser- conser- NN cord-329553-93tbgn2b 337 1 The the DT cord-329553-93tbgn2b 337 2 Journal Journal NNP cord-329553-93tbgn2b 337 3 of of IN cord-329553-93tbgn2b 337 4 Cell Cell NNP cord-329553-93tbgn2b 337 5 Biology Biology NNP cord-329553-93tbgn2b 337 6 , , , cord-329553-93tbgn2b 337 7 Volume volume NN cord-329553-93tbgn2b 337 8 117 117 CD cord-329553-93tbgn2b 337 9 , , , cord-329553-93tbgn2b 337 10 1992 1992 CD cord-329553-93tbgn2b 337 11 vation vation NN cord-329553-93tbgn2b 337 12 in in IN cord-329553-93tbgn2b 337 13 the the DT cord-329553-93tbgn2b 337 14 transmembrane transmembrane NN cord-329553-93tbgn2b 337 15 domain domain NN cord-329553-93tbgn2b 337 16 sequence sequence NN cord-329553-93tbgn2b 337 17 of of IN cord-329553-93tbgn2b 337 18 GT GT NNP cord-329553-93tbgn2b 337 19 and and CC cord-329553-93tbgn2b 337 20 NTI NTI NNP cord-329553-93tbgn2b 337 21 suggests suggest VBZ cord-329553-93tbgn2b 337 22 that that IN cord-329553-93tbgn2b 337 23 they -PRON- PRP cord-329553-93tbgn2b 337 24 might may MD cord-329553-93tbgn2b 337 25 also also RB cord-329553-93tbgn2b 337 26 be be VB cord-329553-93tbgn2b 337 27 involved involve VBN cord-329553-93tbgn2b 337 28 in in IN cord-329553-93tbgn2b 337 29 other other JJ cord-329553-93tbgn2b 337 30 functions function NNS cord-329553-93tbgn2b 337 31 in in IN cord-329553-93tbgn2b 337 32 addition addition NN cord-329553-93tbgn2b 337 33 to to IN cord-329553-93tbgn2b 337 34 their -PRON- PRP$ cord-329553-93tbgn2b 337 35 role role NN cord-329553-93tbgn2b 337 36 in in IN cord-329553-93tbgn2b 337 37 membrane membrane NNP cord-329553-93tbgn2b 337 38 anchorage anchorage NNP cord-329553-93tbgn2b 337 39 . . . cord-329553-93tbgn2b 338 1 Whether whether IN cord-329553-93tbgn2b 338 2 the the DT cord-329553-93tbgn2b 338 3 transmembrane transmembrane NN cord-329553-93tbgn2b 338 4 domain domain NN cord-329553-93tbgn2b 338 5 of of IN cord-329553-93tbgn2b 338 6 GT GT NNP cord-329553-93tbgn2b 338 7 and/or and/or CC cord-329553-93tbgn2b 338 8 NTI NTI NNP cord-329553-93tbgn2b 338 9 is be VBZ cord-329553-93tbgn2b 338 10 similarly similarly RB cord-329553-93tbgn2b 338 11 involved involve VBN cord-329553-93tbgn2b 338 12 in in IN cord-329553-93tbgn2b 338 13 Golgi Golgi NNP cord-329553-93tbgn2b 338 14 localization localization NN cord-329553-93tbgn2b 338 15 needs need VBZ cord-329553-93tbgn2b 338 16 further further JJ cord-329553-93tbgn2b 338 17 investigation investigation NN cord-329553-93tbgn2b 338 18 . . . cord-329553-93tbgn2b 339 1 Our -PRON- PRP$ cord-329553-93tbgn2b 339 2 recent recent JJ cord-329553-93tbgn2b 339 3 studies study NNS cord-329553-93tbgn2b 339 4 with with IN cord-329553-93tbgn2b 339 5 NTI NTI NNP cord-329553-93tbgn2b 339 6 suggest suggest VBP cord-329553-93tbgn2b 339 7 that that IN cord-329553-93tbgn2b 339 8 its -PRON- PRP$ cord-329553-93tbgn2b 339 9 transmembrane transmembrane NN cord-329553-93tbgn2b 339 10 domain domain NN cord-329553-93tbgn2b 339 11 is be VBZ cord-329553-93tbgn2b 339 12 indeed indeed RB cord-329553-93tbgn2b 339 13 involved involve VBN cord-329553-93tbgn2b 339 14 in in IN cord-329553-93tbgn2b 339 15 Golgi Golgi NNP cord-329553-93tbgn2b 339 16 localization localization NN cord-329553-93tbgn2b 339 17 ( ( -LRB- cord-329553-93tbgn2b 339 18 Tang Tang NNP cord-329553-93tbgn2b 339 19 et et FW cord-329553-93tbgn2b 339 20 al al NNP cord-329553-93tbgn2b 339 21 . . NNP cord-329553-93tbgn2b 339 22 , , , cord-329553-93tbgn2b 339 23 1992 1992 CD cord-329553-93tbgn2b 339 24 ) ) -RRB- cord-329553-93tbgn2b 339 25 . . . cord-329553-93tbgn2b 340 1 The the DT cord-329553-93tbgn2b 340 2 sequence sequence NN cord-329553-93tbgn2b 340 3 of of IN cord-329553-93tbgn2b 340 4 the the DT cord-329553-93tbgn2b 340 5 trans trans NN cord-329553-93tbgn2b 340 6 - - NN cord-329553-93tbgn2b 340 7 membrane membrane NN cord-329553-93tbgn2b 340 8 domain domain NN cord-329553-93tbgn2b 340 9 varies vary VBZ cord-329553-93tbgn2b 340 10 greatly greatly RB cord-329553-93tbgn2b 340 11 between between IN cord-329553-93tbgn2b 340 12 ST ST NNP cord-329553-93tbgn2b 340 13 , , , cord-329553-93tbgn2b 340 14 GT GT NNP cord-329553-93tbgn2b 340 15 , , , cord-329553-93tbgn2b 340 16 and and CC cord-329553-93tbgn2b 340 17 NTI NTI NNP cord-329553-93tbgn2b 340 18 , , , cord-329553-93tbgn2b 340 19 and and CC cord-329553-93tbgn2b 340 20 this this DT cord-329553-93tbgn2b 340 21 sequence sequence NN cord-329553-93tbgn2b 340 22 divergence divergence NN cord-329553-93tbgn2b 340 23 of of IN cord-329553-93tbgn2b 340 24 the the DT cord-329553-93tbgn2b 340 25 transmembrane transmembrane NN cord-329553-93tbgn2b 340 26 domain domain NN cord-329553-93tbgn2b 340 27 among among IN cord-329553-93tbgn2b 340 28 different different JJ cord-329553-93tbgn2b 340 29 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 340 30 might may MD cord-329553-93tbgn2b 340 31 be be VB cord-329553-93tbgn2b 340 32 related relate VBN cord-329553-93tbgn2b 340 33 to to IN cord-329553-93tbgn2b 340 34 their -PRON- PRP$ cord-329553-93tbgn2b 340 35 distinct distinct JJ cord-329553-93tbgn2b 340 36 subcompartment subcompartment JJ cord-329553-93tbgn2b 340 37 localization localization NN cord-329553-93tbgn2b 340 38 in in IN cord-329553-93tbgn2b 340 39 the the DT cord-329553-93tbgn2b 340 40 Golgi Golgi NNP cord-329553-93tbgn2b 340 41 apparatus apparatus NN cord-329553-93tbgn2b 340 42 . . . cord-329553-93tbgn2b 341 1 Further further JJ cord-329553-93tbgn2b 341 2 studies study NNS cord-329553-93tbgn2b 341 3 will will MD cord-329553-93tbgn2b 341 4 shed shed VB cord-329553-93tbgn2b 341 5 more more JJR cord-329553-93tbgn2b 341 6 light light NN cord-329553-93tbgn2b 341 7 on on IN cord-329553-93tbgn2b 341 8 this this DT cord-329553-93tbgn2b 341 9 issue issue NN cord-329553-93tbgn2b 341 10 . . . cord-329553-93tbgn2b 342 1 The the DT cord-329553-93tbgn2b 342 2 Golgi Golgi NNP cord-329553-93tbgn2b 342 3 localization localization NN cord-329553-93tbgn2b 342 4 specified specify VBN cord-329553-93tbgn2b 342 5 by by IN cord-329553-93tbgn2b 342 6 the the DT cord-329553-93tbgn2b 342 7 transmembrane transmembrane NN cord-329553-93tbgn2b 342 8 domain domain NN cord-329553-93tbgn2b 342 9 of of IN cord-329553-93tbgn2b 342 10 ST ST NNP cord-329553-93tbgn2b 342 11 could could MD cord-329553-93tbgn2b 342 12 potentially potentially RB cord-329553-93tbgn2b 342 13 be be VB cord-329553-93tbgn2b 342 14 mediated mediate VBN cord-329553-93tbgn2b 342 15 by by IN cord-329553-93tbgn2b 342 16 two two CD cord-329553-93tbgn2b 342 17 different different JJ cord-329553-93tbgn2b 342 18 but but CC cord-329553-93tbgn2b 342 19 related related JJ cord-329553-93tbgn2b 342 20 mechanisms mechanism NNS cord-329553-93tbgn2b 342 21 . . . cord-329553-93tbgn2b 343 1 The the DT cord-329553-93tbgn2b 343 2 membrane membrane NN cord-329553-93tbgn2b 343 3 spanning span VBG cord-329553-93tbgn2b 343 4 domain domain NN cord-329553-93tbgn2b 343 5 may may MD cord-329553-93tbgn2b 343 6 interact interact VB cord-329553-93tbgn2b 343 7 with with IN cord-329553-93tbgn2b 343 8 specific specific JJ cord-329553-93tbgn2b 343 9 lipid lipid NN cord-329553-93tbgn2b 343 10 components component NNS cord-329553-93tbgn2b 343 11 ( ( -LRB- cord-329553-93tbgn2b 343 12 or or CC cord-329553-93tbgn2b 343 13 specific specific JJ cord-329553-93tbgn2b 343 14 lipid lipid NN cord-329553-93tbgn2b 343 15 microenvironment microenvironment NN cord-329553-93tbgn2b 343 16 ) ) -RRB- cord-329553-93tbgn2b 343 17 in in IN cord-329553-93tbgn2b 343 18 the the DT cord-329553-93tbgn2b 343 19 Golgi Golgi NNP cord-329553-93tbgn2b 343 20 membrane membrane NN cord-329553-93tbgn2b 343 21 . . . cord-329553-93tbgn2b 344 1 This this DT cord-329553-93tbgn2b 344 2 interaction interaction NN cord-329553-93tbgn2b 344 3 may may MD cord-329553-93tbgn2b 344 4 immobilize immobilize VB cord-329553-93tbgn2b 344 5 the the DT cord-329553-93tbgn2b 344 6 proteins protein NNS cord-329553-93tbgn2b 344 7 in in IN cord-329553-93tbgn2b 344 8 a a DT cord-329553-93tbgn2b 344 9 particular particular JJ cord-329553-93tbgn2b 344 10 microdomain microdomain NN cord-329553-93tbgn2b 344 11 in in IN cord-329553-93tbgn2b 344 12 the the DT cord-329553-93tbgn2b 344 13 membrane membrane NN cord-329553-93tbgn2b 344 14 . . . cord-329553-93tbgn2b 345 1 Proteins protein NNS cord-329553-93tbgn2b 345 2 in in IN cord-329553-93tbgn2b 345 3 this this DT cord-329553-93tbgn2b 345 4 microdomain microdomain NN cord-329553-93tbgn2b 345 5 may may MD cord-329553-93tbgn2b 345 6 be be VB cord-329553-93tbgn2b 345 7 specifically specifically RB cord-329553-93tbgn2b 345 8 prevented prevent VBN cord-329553-93tbgn2b 345 9 from from IN cord-329553-93tbgn2b 345 10 entry entry NN cord-329553-93tbgn2b 345 11 into into IN cord-329553-93tbgn2b 345 12 the the DT cord-329553-93tbgn2b 345 13 budding bud VBG cord-329553-93tbgn2b 345 14 vesicles vesicle NNS cord-329553-93tbgn2b 345 15 for for IN cord-329553-93tbgn2b 345 16 subsequent subsequent JJ cord-329553-93tbgn2b 345 17 transport transport NN cord-329553-93tbgn2b 345 18 . . . cord-329553-93tbgn2b 346 1 Alternatively alternatively RB cord-329553-93tbgn2b 346 2 , , , cord-329553-93tbgn2b 346 3 the the DT cord-329553-93tbgn2b 346 4 transmembrane transmembrane NN cord-329553-93tbgn2b 346 5 domain domain NN cord-329553-93tbgn2b 346 6 may may MD cord-329553-93tbgn2b 346 7 interact interact VB cord-329553-93tbgn2b 346 8 with with IN cord-329553-93tbgn2b 346 9 a a DT cord-329553-93tbgn2b 346 10 specific specific JJ cord-329553-93tbgn2b 346 11 membrane membrane NN cord-329553-93tbgn2b 346 12 - - HYPH cord-329553-93tbgn2b 346 13 spanning span VBG cord-329553-93tbgn2b 346 14 region region NN cord-329553-93tbgn2b 346 15 of of IN cord-329553-93tbgn2b 346 16 other other JJ cord-329553-93tbgn2b 346 17 proteins protein NNS cord-329553-93tbgn2b 346 18 that that WDT cord-329553-93tbgn2b 346 19 are be VBP cord-329553-93tbgn2b 346 20 already already RB cord-329553-93tbgn2b 346 21 immobilized immobilize VBN cord-329553-93tbgn2b 346 22 in in IN cord-329553-93tbgn2b 346 23 the the DT cord-329553-93tbgn2b 346 24 Golgi Golgi NNP cord-329553-93tbgn2b 346 25 membrane membrane NN cord-329553-93tbgn2b 346 26 through through IN cord-329553-93tbgn2b 346 27 unknown unknown JJ cord-329553-93tbgn2b 346 28 mechanisms mechanism NNS cord-329553-93tbgn2b 346 29 . . . cord-329553-93tbgn2b 347 1 Furthermore furthermore RB cord-329553-93tbgn2b 347 2 , , , cord-329553-93tbgn2b 347 3 interaction interaction NN cord-329553-93tbgn2b 347 4 of of IN cord-329553-93tbgn2b 347 5 the the DT cord-329553-93tbgn2b 347 6 transmembrane transmembrane NN cord-329553-93tbgn2b 347 7 domains domain NNS cord-329553-93tbgn2b 347 8 among among IN cord-329553-93tbgn2b 347 9 different different JJ cord-329553-93tbgn2b 347 10 molecules molecule NNS cord-329553-93tbgn2b 347 11 of of IN cord-329553-93tbgn2b 347 12 ST ST NNP cord-329553-93tbgn2b 347 13 ( ( -LRB- cord-329553-93tbgn2b 347 14 self self NN cord-329553-93tbgn2b 347 15 interaction interaction NN cord-329553-93tbgn2b 347 16 ) ) -RRB- cord-329553-93tbgn2b 347 17 induced induce VBN cord-329553-93tbgn2b 347 18 by by IN cord-329553-93tbgn2b 347 19 the the DT cord-329553-93tbgn2b 347 20 Golgi Golgi NNP cord-329553-93tbgn2b 347 21 environment environment NN cord-329553-93tbgn2b 347 22 may may MD cord-329553-93tbgn2b 347 23 also also RB cord-329553-93tbgn2b 347 24 be be VB cord-329553-93tbgn2b 347 25 involved involve VBN cord-329553-93tbgn2b 347 26 . . . cord-329553-93tbgn2b 348 1 At at IN cord-329553-93tbgn2b 348 2 present present NN cord-329553-93tbgn2b 348 3 , , , cord-329553-93tbgn2b 348 4 we -PRON- PRP cord-329553-93tbgn2b 348 5 are be VBP cord-329553-93tbgn2b 348 6 unable unable JJ cord-329553-93tbgn2b 348 7 to to TO cord-329553-93tbgn2b 348 8 propose propose VB cord-329553-93tbgn2b 348 9 a a DT cord-329553-93tbgn2b 348 10 detailed detailed JJ cord-329553-93tbgn2b 348 11 molecular molecular JJ cord-329553-93tbgn2b 348 12 mechanism mechanism NN cord-329553-93tbgn2b 348 13 to to TO cord-329553-93tbgn2b 348 14 explain explain VB cord-329553-93tbgn2b 348 15 the the DT cord-329553-93tbgn2b 348 16 Golgi Golgi NNP cord-329553-93tbgn2b 348 17 localization localization NN cord-329553-93tbgn2b 348 18 mediated mediate VBN cord-329553-93tbgn2b 348 19 by by IN cord-329553-93tbgn2b 348 20 the the DT cord-329553-93tbgn2b 348 21 ST ST NNP cord-329553-93tbgn2b 348 22 transmembrane transmembrane NNP cord-329553-93tbgn2b 348 23 domain domain NN cord-329553-93tbgn2b 348 24 . . . cord-329553-93tbgn2b 349 1 Future future JJ cord-329553-93tbgn2b 349 2 studies study NNS cord-329553-93tbgn2b 349 3 with with IN cord-329553-93tbgn2b 349 4 point point NN cord-329553-93tbgn2b 349 5 mutations mutation NNS cord-329553-93tbgn2b 349 6 in in IN cord-329553-93tbgn2b 349 7 the the DT cord-329553-93tbgn2b 349 8 ST ST NNP cord-329553-93tbgn2b 349 9 transmembrane transmembrane NNP cord-329553-93tbgn2b 349 10 domain domain NN cord-329553-93tbgn2b 349 11 and and CC cord-329553-93tbgn2b 349 12 other other JJ cord-329553-93tbgn2b 349 13 glycosyltransferases glycosyltransferase NNS cord-329553-93tbgn2b 349 14 will will MD cord-329553-93tbgn2b 349 15 enable enable VB cord-329553-93tbgn2b 349 16 us -PRON- PRP cord-329553-93tbgn2b 349 17 to to TO cord-329553-93tbgn2b 349 18 gain gain VB cord-329553-93tbgn2b 349 19 more more JJR cord-329553-93tbgn2b 349 20 understanding understanding NN cord-329553-93tbgn2b 349 21 about about IN cord-329553-93tbgn2b 349 22 the the DT cord-329553-93tbgn2b 349 23 cellular cellular JJ cord-329553-93tbgn2b 349 24 targeting targeting NN cord-329553-93tbgn2b 349 25 mediated mediate VBN cord-329553-93tbgn2b 349 26 by by IN cord-329553-93tbgn2b 349 27 the the DT cord-329553-93tbgn2b 349 28 transmembrane transmembrane NN cord-329553-93tbgn2b 349 29 domain domain NN cord-329553-93tbgn2b 349 30 . . . cord-329553-93tbgn2b 350 1 Cellular cellular JJ cord-329553-93tbgn2b 350 2 localization localization NN cord-329553-93tbgn2b 350 3 determined determine VBN cord-329553-93tbgn2b 350 4 by by IN cord-329553-93tbgn2b 350 5 a a DT cord-329553-93tbgn2b 350 6 membrane membrane NN cord-329553-93tbgn2b 350 7 anchoring anchor VBG cord-329553-93tbgn2b 350 8 region region NN cord-329553-93tbgn2b 350 9 has have VBZ cord-329553-93tbgn2b 350 10 been be VBN cord-329553-93tbgn2b 350 11 described describe VBN cord-329553-93tbgn2b 350 12 for for IN cord-329553-93tbgn2b 350 13 other other JJ cord-329553-93tbgn2b 350 14 proteins protein NNS cord-329553-93tbgn2b 350 15 . . . cord-329553-93tbgn2b 351 1 Recent recent JJ cord-329553-93tbgn2b 351 2 studies study NNS cord-329553-93tbgn2b 351 3 have have VBP cord-329553-93tbgn2b 351 4 established establish VBN cord-329553-93tbgn2b 351 5 that that IN cord-329553-93tbgn2b 351 6 the the DT cord-329553-93tbgn2b 351 7 GPI GPI NNP cord-329553-93tbgn2b 351 8 lipid lipid NN cord-329553-93tbgn2b 351 9 anchor anchor NN cord-329553-93tbgn2b 351 10 functions function NNS cord-329553-93tbgn2b 351 11 as as IN cord-329553-93tbgn2b 351 12 a a DT cord-329553-93tbgn2b 351 13 targeting targeting JJ cord-329553-93tbgn2b 351 14 signal signal NN cord-329553-93tbgn2b 351 15 for for IN cord-329553-93tbgn2b 351 16 the the DT cord-329553-93tbgn2b 351 17 apical apical JJ cord-329553-93tbgn2b 351 18 plasma plasma NN cord-329553-93tbgn2b 351 19 membrane membrane NN cord-329553-93tbgn2b 351 20 domain domain NN cord-329553-93tbgn2b 351 21 in in IN cord-329553-93tbgn2b 351 22 epithelial epithelial JJ cord-329553-93tbgn2b 351 23 cells cell NNS cord-329553-93tbgn2b 352 1 Lisanti Lisanti NNP cord-329553-93tbgn2b 352 2 et et NNP cord-329553-93tbgn2b 352 3 al al NNP cord-329553-93tbgn2b 352 4 . . NNP cord-329553-93tbgn2b 352 5 , , , cord-329553-93tbgn2b 352 6 1989 1989 CD cord-329553-93tbgn2b 352 7 ) ) -RRB- cord-329553-93tbgn2b 352 8 . . . cord-329553-93tbgn2b 353 1 Furthermore furthermore RB cord-329553-93tbgn2b 353 2 , , , cord-329553-93tbgn2b 353 3 the the DT cord-329553-93tbgn2b 353 4 transmembrane transmembrane NN cord-329553-93tbgn2b 353 5 domain domain NN cord-329553-93tbgn2b 353 6 of of IN cord-329553-93tbgn2b 353 7 the the DT cord-329553-93tbgn2b 353 8 T T NNP cord-329553-93tbgn2b 353 9 cell cell NN cord-329553-93tbgn2b 353 10 antigen antigen NN cord-329553-93tbgn2b 353 11 receptor receptor NN cord-329553-93tbgn2b 353 12 a a DT cord-329553-93tbgn2b 353 13 - - HYPH cord-329553-93tbgn2b 353 14 subunit subunit NN cord-329553-93tbgn2b 353 15 has have VBZ cord-329553-93tbgn2b 353 16 been be VBN cord-329553-93tbgn2b 353 17 sown sow VBN cord-329553-93tbgn2b 353 18 to to TO cord-329553-93tbgn2b 353 19 be be VB cord-329553-93tbgn2b 353 20 sufficient sufficient JJ cord-329553-93tbgn2b 353 21 for for IN cord-329553-93tbgn2b 353 22 ER ER NNP cord-329553-93tbgn2b 353 23 degradation degradation NN cord-329553-93tbgn2b 353 24 and and CC cord-329553-93tbgn2b 353 25 subunit subunit NN cord-329553-93tbgn2b 353 26 assembly assembly NN cord-329553-93tbgn2b 353 27 of of IN cord-329553-93tbgn2b 353 28 the the DT cord-329553-93tbgn2b 353 29 T T NNP cord-329553-93tbgn2b 353 30 cell cell NN cord-329553-93tbgn2b 353 31 antigen antigen NN cord-329553-93tbgn2b 353 32 receptor receptor NN cord-329553-93tbgn2b 353 33 ( ( -LRB- cord-329553-93tbgn2b 353 34 Bonifacino Bonifacino NNP cord-329553-93tbgn2b 353 35 et et FW cord-329553-93tbgn2b 353 36 al al NNP cord-329553-93tbgn2b 353 37 . . NNP cord-329553-93tbgn2b 353 38 , , , cord-329553-93tbgn2b 353 39 1990 1990 CD cord-329553-93tbgn2b 353 40 ) ) -RRB- cord-329553-93tbgn2b 353 41 . . . cord-329553-93tbgn2b 354 1 Studies study NNS cord-329553-93tbgn2b 354 2 with with IN cord-329553-93tbgn2b 354 3 the the DT cord-329553-93tbgn2b 354 4 El El NNP cord-329553-93tbgn2b 354 5 protein protein NN cord-329553-93tbgn2b 354 6 of of IN cord-329553-93tbgn2b 354 7 avian avian JJ cord-329553-93tbgn2b 354 8 coronavirus coronavirus NN cord-329553-93tbgn2b 354 9 infectious infectious JJ cord-329553-93tbgn2b 354 10 bronchitis bronchitis NN cord-329553-93tbgn2b 354 11 virus virus NN cord-329553-93tbgn2b 354 12 , , , cord-329553-93tbgn2b 354 13 a a DT cord-329553-93tbgn2b 354 14 cis cis NNP cord-329553-93tbgn2b 354 15 - - HYPH cord-329553-93tbgn2b 354 16 Golgi Golgi NNP cord-329553-93tbgn2b 354 17 protein protein NN cord-329553-93tbgn2b 354 18 ( ( -LRB- cord-329553-93tbgn2b 354 19 Machamer Machamer NNP cord-329553-93tbgn2b 354 20 et et NNP cord-329553-93tbgn2b 354 21 al al NNP cord-329553-93tbgn2b 354 22 . . NNP cord-329553-93tbgn2b 354 23 , , , cord-329553-93tbgn2b 354 24 1990 1990 CD cord-329553-93tbgn2b 354 25 ) ) -RRB- cord-329553-93tbgn2b 354 26 , , , cord-329553-93tbgn2b 354 27 demonstrated demonstrate VBD cord-329553-93tbgn2b 354 28 that that IN cord-329553-93tbgn2b 354 29 the the DT cord-329553-93tbgn2b 354 30 first first JJ cord-329553-93tbgn2b 354 31 of of IN cord-329553-93tbgn2b 354 32 its -PRON- PRP$ cord-329553-93tbgn2b 354 33 three three CD cord-329553-93tbgn2b 354 34 transmembrane transmembrane NN cord-329553-93tbgn2b 354 35 domains domain NNS cord-329553-93tbgn2b 354 36 is be VBZ cord-329553-93tbgn2b 354 37 necessary necessary JJ cord-329553-93tbgn2b 354 38 and and CC cord-329553-93tbgn2b 354 39 sufficient sufficient JJ cord-329553-93tbgn2b 354 40 for for IN cord-329553-93tbgn2b 354 41 Golgi Golgi NNP cord-329553-93tbgn2b 354 42 localization localization NN cord-329553-93tbgn2b 354 43 ( ( -LRB- cord-329553-93tbgn2b 354 44 Machamer Machamer NNP cord-329553-93tbgn2b 354 45 and and CC cord-329553-93tbgn2b 354 46 Rose Rose NNP cord-329553-93tbgn2b 354 47 , , , cord-329553-93tbgn2b 354 48 1987 1987 CD cord-329553-93tbgn2b 354 49 ; ; : cord-329553-93tbgn2b 354 50 Swift Swift NNP cord-329553-93tbgn2b 354 51 and and CC cord-329553-93tbgn2b 354 52 Machamer Machamer NNP cord-329553-93tbgn2b 354 53 , , , cord-329553-93tbgn2b 354 54 1991 1991 CD cord-329553-93tbgn2b 354 55 ) ) -RRB- cord-329553-93tbgn2b 354 56 , , , cord-329553-93tbgn2b 354 57 although although IN cord-329553-93tbgn2b 354 58 different different JJ cord-329553-93tbgn2b 354 59 results result NNS cord-329553-93tbgn2b 354 60 were be VBD cord-329553-93tbgn2b 354 61 obtained obtain VBN cord-329553-93tbgn2b 354 62 with with IN cord-329553-93tbgn2b 354 63 the the DT cord-329553-93tbgn2b 354 64 El El NNP cord-329553-93tbgn2b 354 65 protein protein NN cord-329553-93tbgn2b 354 66 of of IN cord-329553-93tbgn2b 354 67 another another DT cord-329553-93tbgn2b 354 68 coronavirus coronavirus NN cord-329553-93tbgn2b 354 69 ( ( -LRB- cord-329553-93tbgn2b 354 70 mouse mouse NN cord-329553-93tbgn2b 354 71 hepatitis hepatitis NN cord-329553-93tbgn2b 354 72 virus virus NN cord-329553-93tbgn2b 354 73 A59 A59 NNP cord-329553-93tbgn2b 354 74 ) ) -RRB- cord-329553-93tbgn2b 355 1 ( ( -LRB- cord-329553-93tbgn2b 355 2 Armstrong Armstrong NNP cord-329553-93tbgn2b 355 3 et et NNP cord-329553-93tbgn2b 355 4 al al NNP cord-329553-93tbgn2b 355 5 . . NNP cord-329553-93tbgn2b 355 6 , , , cord-329553-93tbgn2b 355 7 1990 1990 CD cord-329553-93tbgn2b 355 8 ; ; : cord-329553-93tbgn2b 356 1 Armstrong Armstrong NNP cord-329553-93tbgn2b 356 2 and and CC cord-329553-93tbgn2b 356 3 Patel Patel NNP cord-329553-93tbgn2b 356 4 , , , cord-329553-93tbgn2b 356 5 1991 1991 CD cord-329553-93tbgn2b 356 6 ) ) -RRB- cord-329553-93tbgn2b 356 7 . . . cord-329553-93tbgn2b 357 1 These these DT cord-329553-93tbgn2b 357 2 observations observation NNS cord-329553-93tbgn2b 357 3 suggest suggest VBP cord-329553-93tbgn2b 357 4 that that IN cord-329553-93tbgn2b 357 5 the the DT cord-329553-93tbgn2b 357 6 subcellular subcellular JJ cord-329553-93tbgn2b 357 7 destination destination NN cord-329553-93tbgn2b 357 8 of of IN cord-329553-93tbgn2b 357 9 some some DT cord-329553-93tbgn2b 357 10 integral integral JJ cord-329553-93tbgn2b 357 11 membrane membrane NN cord-329553-93tbgn2b 357 12 proteins protein NNS cord-329553-93tbgn2b 357 13 is be VBZ cord-329553-93tbgn2b 357 14 signaled signal VBN cord-329553-93tbgn2b 357 15 by by IN cord-329553-93tbgn2b 357 16 their -PRON- PRP$ cord-329553-93tbgn2b 357 17 membrane membrane NN cord-329553-93tbgn2b 357 18 - - HYPH cord-329553-93tbgn2b 357 19 spanning span VBG cord-329553-93tbgn2b 357 20 regions region NNS cord-329553-93tbgn2b 357 21 . . . cord-329553-93tbgn2b 358 1 During during IN cord-329553-93tbgn2b 358 2 the the DT cord-329553-93tbgn2b 358 3 review review NN cord-329553-93tbgn2b 358 4 of of IN cord-329553-93tbgn2b 358 5 this this DT cord-329553-93tbgn2b 358 6 manuscript manuscript NN cord-329553-93tbgn2b 358 7 , , , cord-329553-93tbgn2b 358 8 two two CD cord-329553-93tbgn2b 358 9 papers paper NNS cord-329553-93tbgn2b 358 10 were be VBD cord-329553-93tbgn2b 358 11 published publish VBN cord-329553-93tbgn2b 358 12 which which WDT cord-329553-93tbgn2b 358 13 demonstrated demonstrate VBD cord-329553-93tbgn2b 358 14 that that IN cord-329553-93tbgn2b 358 15 the the DT cord-329553-93tbgn2b 358 16 membrane membrane NN cord-329553-93tbgn2b 358 17 - - HYPH cord-329553-93tbgn2b 358 18 spanning span VBG cord-329553-93tbgn2b 358 19 domain domain NN cord-329553-93tbgn2b 358 20 of of IN cord-329553-93tbgn2b 358 21 GT GT NNP cord-329553-93tbgn2b 358 22 ( ( -LRB- cord-329553-93tbgn2b 358 23 Nilsson Nilsson NNP cord-329553-93tbgn2b 358 24 et et NNP cord-329553-93tbgn2b 358 25 al al NNP cord-329553-93tbgn2b 358 26 . . NNP cord-329553-93tbgn2b 358 27 , , , cord-329553-93tbgn2b 358 28 1991 1991 CD cord-329553-93tbgn2b 358 29 ) ) -RRB- cord-329553-93tbgn2b 358 30 and and CC cord-329553-93tbgn2b 358 31 the the DT cord-329553-93tbgn2b 358 32 transmembrane transmembrane NN cord-329553-93tbgn2b 358 33 domain domain NN cord-329553-93tbgn2b 358 34 and and CC cord-329553-93tbgn2b 358 35 its -PRON- PRP$ cord-329553-93tbgn2b 358 36 flanking flanking JJ cord-329553-93tbgn2b 358 37 sequences sequence NNS cord-329553-93tbgn2b 358 38 of of IN cord-329553-93tbgn2b 358 39 ST ST NNP cord-329553-93tbgn2b 358 40 ( ( -LRB- cord-329553-93tbgn2b 358 41 Munro Munro NNP cord-329553-93tbgn2b 358 42 , , , cord-329553-93tbgn2b 358 43 1991 1991 CD cord-329553-93tbgn2b 358 44 ) ) -RRB- cord-329553-93tbgn2b 358 45 could could MD cord-329553-93tbgn2b 358 46 specify specify VB cord-329553-93tbgn2b 358 47 Golgi Golgi NNP cord-329553-93tbgn2b 358 48 localization localization NN cord-329553-93tbgn2b 358 49 . . . cord-329553-93tbgn2b 359 1 Although although IN cord-329553-93tbgn2b 359 2 similar similar JJ cord-329553-93tbgn2b 359 3 conclusions conclusion NNS cord-329553-93tbgn2b 359 4 were be VBD cord-329553-93tbgn2b 359 5 reached reach VBN cord-329553-93tbgn2b 359 6 by by IN cord-329553-93tbgn2b 359 7 Munro Munro NNP cord-329553-93tbgn2b 359 8 in in IN cord-329553-93tbgn2b 359 9 his -PRON- PRP$ cord-329553-93tbgn2b 359 10 study study NN cord-329553-93tbgn2b 359 11 , , , cord-329553-93tbgn2b 359 12 there there EX cord-329553-93tbgn2b 359 13 were be VBD cord-329553-93tbgn2b 359 14 a a DT cord-329553-93tbgn2b 359 15 few few JJ cord-329553-93tbgn2b 359 16 significant significant JJ cord-329553-93tbgn2b 359 17 differences difference NNS cord-329553-93tbgn2b 359 18 in in IN cord-329553-93tbgn2b 359 19 the the DT cord-329553-93tbgn2b 359 20 approach approach NN cord-329553-93tbgn2b 359 21 and and CC cord-329553-93tbgn2b 359 22 results result NNS cord-329553-93tbgn2b 359 23 presented present VBN cord-329553-93tbgn2b 359 24 from from IN cord-329553-93tbgn2b 359 25 our -PRON- PRP$ cord-329553-93tbgn2b 359 26 study study NN cord-329553-93tbgn2b 359 27 . . . cord-329553-93tbgn2b 360 1 ( ( -LRB- cord-329553-93tbgn2b 360 2 a a LS cord-329553-93tbgn2b 360 3 ) ) -RRB- cord-329553-93tbgn2b 360 4 Munro Munro NNP cord-329553-93tbgn2b 360 5 used use VBD cord-329553-93tbgn2b 360 6 a a DT cord-329553-93tbgn2b 360 7 transient transient JJ cord-329553-93tbgn2b 360 8 expression expression NN cord-329553-93tbgn2b 360 9 system system NN cord-329553-93tbgn2b 360 10 in in IN cord-329553-93tbgn2b 360 11 COS COS NNP cord-329553-93tbgn2b 360 12 cells cell NNS cord-329553-93tbgn2b 360 13 while while IN cord-329553-93tbgn2b 360 14 our -PRON- PRP$ cord-329553-93tbgn2b 360 15 study study NN cord-329553-93tbgn2b 360 16 was be VBD cord-329553-93tbgn2b 360 17 performed perform VBN cord-329553-93tbgn2b 360 18 in in IN cord-329553-93tbgn2b 360 19 stably stably RB cord-329553-93tbgn2b 360 20 transfected transfecte VBN cord-329553-93tbgn2b 360 21 MDCK MDCK NNP cord-329553-93tbgn2b 360 22 cells cell NNS cord-329553-93tbgn2b 360 23 . . . cord-329553-93tbgn2b 361 1 ( ( -LRB- cord-329553-93tbgn2b 361 2 b b LS cord-329553-93tbgn2b 361 3 ) ) -RRB- cord-329553-93tbgn2b 361 4 Our -PRON- PRP$ cord-329553-93tbgn2b 361 5 results result NNS cord-329553-93tbgn2b 361 6 clearly clearly RB cord-329553-93tbgn2b 361 7 demonstrate demonstrate VBP cord-329553-93tbgn2b 361 8 that that IN cord-329553-93tbgn2b 361 9 the the DT cord-329553-93tbgn2b 361 10 17-residue 17-residue CD cord-329553-93tbgn2b 361 11 transmembrane transmembrane NN cord-329553-93tbgn2b 361 12 domain domain NN cord-329553-93tbgn2b 361 13 itself -PRON- PRP cord-329553-93tbgn2b 361 14 was be VBD cord-329553-93tbgn2b 361 15 sufficient sufficient JJ cord-329553-93tbgn2b 361 16 for for IN cord-329553-93tbgn2b 361 17 Golgi Golgi NNP cord-329553-93tbgn2b 361 18 localization localization NN cord-329553-93tbgn2b 361 19 of of IN cord-329553-93tbgn2b 361 20 the the DT cord-329553-93tbgn2b 361 21 chimeric chimeric JJ cord-329553-93tbgn2b 361 22 protein protein NN cord-329553-93tbgn2b 361 23 and and CC cord-329553-93tbgn2b 361 24 its -PRON- PRP$ cord-329553-93tbgn2b 361 25 flanking flanking JJ cord-329553-93tbgn2b 361 26 sequences sequence NNS cord-329553-93tbgn2b 361 27 did do VBD cord-329553-93tbgn2b 361 28 not not RB cord-329553-93tbgn2b 361 29 contribute contribute VB cord-329553-93tbgn2b 361 30 much much JJ cord-329553-93tbgn2b 361 31 to to IN cord-329553-93tbgn2b 361 32 the the DT cord-329553-93tbgn2b 361 33 efficiency efficiency NN cord-329553-93tbgn2b 361 34 of of IN cord-329553-93tbgn2b 361 35 Golgi Golgi NNP cord-329553-93tbgn2b 361 36 localization localization NN cord-329553-93tbgn2b 361 37 . . . cord-329553-93tbgn2b 362 1 This this DT cord-329553-93tbgn2b 362 2 difference difference NN cord-329553-93tbgn2b 362 3 could could MD cord-329553-93tbgn2b 362 4 be be VB cord-329553-93tbgn2b 362 5 due due JJ cord-329553-93tbgn2b 362 6 to to IN cord-329553-93tbgn2b 362 7 the the DT cord-329553-93tbgn2b 362 8 different different JJ cord-329553-93tbgn2b 362 9 expression expression NN cord-329553-93tbgn2b 362 10 systems system NNS cord-329553-93tbgn2b 362 11 used use VBN cord-329553-93tbgn2b 362 12 and and CC cord-329553-93tbgn2b 362 13 the the DT cord-329553-93tbgn2b 362 14 fact fact NN cord-329553-93tbgn2b 362 15 that that IN cord-329553-93tbgn2b 362 16 our -PRON- PRP$ cord-329553-93tbgn2b 362 17 chimeric chimeric JJ cord-329553-93tbgn2b 362 18 proteins protein NNS cord-329553-93tbgn2b 362 19 were be VBD cord-329553-93tbgn2b 362 20 derived derive VBN cord-329553-93tbgn2b 362 21 from from IN cord-329553-93tbgn2b 362 22 two two CD cord-329553-93tbgn2b 362 23 well well RB cord-329553-93tbgn2b 362 24 - - HYPH cord-329553-93tbgn2b 362 25 defined define VBN cord-329553-93tbgn2b 362 26 cellular cellular JJ cord-329553-93tbgn2b 362 27 proteins protein NNS cord-329553-93tbgn2b 362 28 of of IN cord-329553-93tbgn2b 362 29 the the DT cord-329553-93tbgn2b 362 30 same same JJ cord-329553-93tbgn2b 362 31 membrane membrane NN cord-329553-93tbgn2b 362 32 topology topology NN cord-329553-93tbgn2b 362 33 while while IN cord-329553-93tbgn2b 362 34 those those DT cord-329553-93tbgn2b 362 35 in in IN cord-329553-93tbgn2b 362 36 Munro Munro NNP cord-329553-93tbgn2b 362 37 's 's POS cord-329553-93tbgn2b 362 38 study study NN cord-329553-93tbgn2b 362 39 involved involve VBD cord-329553-93tbgn2b 362 40 a a DT cord-329553-93tbgn2b 362 41 tripartite tripartite JJ cord-329553-93tbgn2b 362 42 fusion fusion NN cord-329553-93tbgn2b 362 43 among among IN cord-329553-93tbgn2b 362 44 three three CD cord-329553-93tbgn2b 362 45 proteins protein NNS cord-329553-93tbgn2b 362 46 . . . cord-329553-93tbgn2b 363 1 ( ( -LRB- cord-329553-93tbgn2b 363 2 c c NN cord-329553-93tbgn2b 363 3 ) ) -RRB- cord-329553-93tbgn2b 364 1 In in IN cord-329553-93tbgn2b 364 2 addition addition NN cord-329553-93tbgn2b 364 3 to to IN cord-329553-93tbgn2b 364 4 confirming confirm VBG cord-329553-93tbgn2b 364 5 biochemically biochemically RB cord-329553-93tbgn2b 364 6 the the DT cord-329553-93tbgn2b 364 7 Golgi Golgi NNP cord-329553-93tbgn2b 364 8 localization localization NN cord-329553-93tbgn2b 364 9 of of IN cord-329553-93tbgn2b 364 10 the the DT cord-329553-93tbgn2b 364 11 chimeric chimeric JJ cord-329553-93tbgn2b 364 12 proteins protein NNS cord-329553-93tbgn2b 364 13 , , , cord-329553-93tbgn2b 364 14 we -PRON- PRP cord-329553-93tbgn2b 364 15 also also RB cord-329553-93tbgn2b 364 16 used use VBD cord-329553-93tbgn2b 364 17 surface surface NN cord-329553-93tbgn2b 364 18 biotinylation biotinylation NN cord-329553-93tbgn2b 364 19 to to TO cord-329553-93tbgn2b 364 20 demonstrate demonstrate VB cord-329553-93tbgn2b 364 21 that that IN cord-329553-93tbgn2b 364 22 the the DT cord-329553-93tbgn2b 364 23 Golgi Golgi NNP cord-329553-93tbgn2b 364 24 - - HYPH cord-329553-93tbgn2b 364 25 localizedchimeric localizedchimeric NN cord-329553-93tbgn2b 364 26 proteins protein NNS cord-329553-93tbgn2b 364 27 were be VBD cord-329553-93tbgn2b 364 28 not not RB cord-329553-93tbgn2b 364 29 initially initially RB cord-329553-93tbgn2b 364 30 transported transport VBN cord-329553-93tbgn2b 364 31 to to IN cord-329553-93tbgn2b 364 32 the the DT cord-329553-93tbgn2b 364 33 cell cell NN cord-329553-93tbgn2b 364 34 surface surface NN cord-329553-93tbgn2b 364 35 followed follow VBN cord-329553-93tbgn2b 364 36 by by IN cord-329553-93tbgn2b 364 37 recycling recycle VBG cord-329553-93tbgn2b 364 38 back back RB cord-329553-93tbgn2b 364 39 to to IN cord-329553-93tbgn2b 364 40 the the DT cord-329553-93tbgn2b 364 41 Golgi Golgi NNP cord-329553-93tbgn2b 364 42 , , , cord-329553-93tbgn2b 364 43 providing provide VBG cord-329553-93tbgn2b 364 44 the the DT cord-329553-93tbgn2b 364 45 first first JJ cord-329553-93tbgn2b 364 46 evidence evidence NN cord-329553-93tbgn2b 364 47 that that IN cord-329553-93tbgn2b 364 48 they -PRON- PRP cord-329553-93tbgn2b 364 49 were be VBD cord-329553-93tbgn2b 364 50 selectively selectively RB cord-329553-93tbgn2b 364 51 retained retain VBN cord-329553-93tbgn2b 364 52 in in IN cord-329553-93tbgn2b 364 53 the the DT cord-329553-93tbgn2b 364 54 Golgi Golgi NNP cord-329553-93tbgn2b 364 55 complex complex NN cord-329553-93tbgn2b 364 56 . . . cord-329553-93tbgn2b 365 1 The the DT cord-329553-93tbgn2b 365 2 Golgi Golgi NNP cord-329553-93tbgn2b 365 3 sorting sorting NN cord-329553-93tbgn2b 365 4 domain domain NN cord-329553-93tbgn2b 365 5 of of IN cord-329553-93tbgn2b 365 6 coronavirus coronavirus NN cord-329553-93tbgn2b 365 7 El El NNP cord-329553-93tbgn2b 365 8 protein protein NN cord-329553-93tbgn2b 365 9 Lysosomal Lysosomal NNP cord-329553-93tbgn2b 365 10 sorting sort VBG cord-329553-93tbgn2b 365 11 mutants mutant NNS cord-329553-93tbgn2b 365 12 of of IN cord-329553-93tbgn2b 365 13 coronavirus coronavirus NN cord-329553-93tbgn2b 365 14 El El NNP cord-329553-93tbgn2b 365 15 protein protein NN cord-329553-93tbgn2b 365 16 , , , cord-329553-93tbgn2b 365 17 a a DT cord-329553-93tbgn2b 365 18 Golgi Golgi NNP cord-329553-93tbgn2b 365 19 membrane membrane NN cord-329553-93tbgn2b 365 20 protein protein NN cord-329553-93tbgn2b 365 21 A a DT cord-329553-93tbgn2b 365 22 common common JJ cord-329553-93tbgn2b 365 23 peptide peptide NN cord-329553-93tbgn2b 365 24 stretch stretch NN cord-329553-93tbgn2b 365 25 among among IN cord-329553-93tbgn2b 365 26 enzymes enzyme NNS cord-329553-93tbgn2b 365 27 localized localize VBN cord-329553-93tbgn2b 365 28 to to IN cord-329553-93tbgn2b 365 29 the the DT cord-329553-93tbgn2b 365 30 Golgi Golgi NNP cord-329553-93tbgn2b 365 31 apparatus apparatus NN cord-329553-93tbgn2b 365 32 : : : cord-329553-93tbgn2b 365 33 structural structural JJ cord-329553-93tbgn2b 365 34 similarity similarity NN cord-329553-93tbgn2b 365 35 of of IN cord-329553-93tbgn2b 365 36 Golgi Golgi NNP cord-329553-93tbgn2b 365 37 - - HYPH cord-329553-93tbgn2b 365 38 associated associate VBN cord-329553-93tbgn2b 365 39 glycosyltransferase glycosyltransferase NN cord-329553-93tbgn2b 365 40 Colocalized Colocalized NNP cord-329553-93tbgn2b 365 41 transmembrane transmembrane NN cord-329553-93tbgn2b 365 42 determinants determinant NNS cord-329553-93tbgn2b 365 43 for for IN cord-329553-93tbgn2b 365 44 ER ER NNP cord-329553-93tbgn2b 365 45 degradation degradation NN cord-329553-93tbgn2b 365 46 and and CC cord-329553-93tbgn2b 365 47 subunit subunit NN cord-329553-93tbgn2b 365 48 assembly assembly NN cord-329553-93tbgn2b 365 49 explain explain VBP cord-329553-93tbgn2b 365 50 the the DT cord-329553-93tbgn2b 365 51 intracellular intracellular JJ cord-329553-93tbgn2b 365 52 fate fate NN cord-329553-93tbgn2b 365 53 of of IN cord-329553-93tbgn2b 365 54 TCR TCR NNP cord-329553-93tbgn2b 365 55 chains chain NNS cord-329553-93tbgn2b 366 1 A a DT cord-329553-93tbgn2b 366 2 new new JJ cord-329553-93tbgn2b 366 3 method method NN cord-329553-93tbgn2b 366 4 for for IN cord-329553-93tbgn2b 366 5 detecting detect VBG cord-329553-93tbgn2b 366 6 endocytosed endocytose VBN cord-329553-93tbgn2b 366 7 proteins protein NNS cord-329553-93tbgn2b 366 8 Mechanism Mechanism NNP cord-329553-93tbgn2b 366 9 of of IN cord-329553-93tbgn2b 366 10 membrane membrane NN cord-329553-93tbgn2b 366 11 anchoring anchoring NN cord-329553-93tbgn2b 366 12 affects affect VBZ cord-329553-93tbgn2b 366 13 polarized polarized JJ cord-329553-93tbgn2b 366 14 expression expression NN cord-329553-93tbgn2b 366 15 of of IN cord-329553-93tbgn2b 366 16 two two CD cord-329553-93tbgn2b 366 17 proteins protein NNS cord-329553-93tbgn2b 366 18 in in IN cord-329553-93tbgn2b 366 19 MDCK MDCK NNP cord-329553-93tbgn2b 366 20 cells cell NNS cord-329553-93tbgn2b 366 21 Compartmentation Compartmentation NNP cord-329553-93tbgn2b 366 22 of of IN cord-329553-93tbgn2b 366 23 the the DT cord-329553-93tbgn2b 366 24 Golgi Golgi NNP cord-329553-93tbgn2b 366 25 complex complex NN cord-329553-93tbgn2b 366 26 : : : cord-329553-93tbgn2b 366 27 brefeldin brefeldin NNP cord-329553-93tbgn2b 366 28 - - HYPH cord-329553-93tbgn2b 366 29 A A NNP cord-329553-93tbgn2b 366 30 distinguishes distinguish VBZ cord-329553-93tbgn2b 366 31 trans trans NNP cord-329553-93tbgn2b 366 32 - - JJ cord-329553-93tbgn2b 366 33 Golgi Golgi NNP cord-329553-93tbgn2b 366 34 cistemae cistemae NN cord-329553-93tbgn2b 366 35 from from IN cord-329553-93tbgn2b 366 36 the the DT cord-329553-93tbgn2b 366 37 trans trans NNP cord-329553-93tbgn2b 366 38 - - NNP cord-329553-93tbgn2b 366 39 Golgi Golgi NNP cord-329553-93tbgn2b 366 40 network network NN cord-329553-93tbgn2b 366 41 Conversion conversion NN cord-329553-93tbgn2b 366 42 of of IN cord-329553-93tbgn2b 366 43 a a DT cord-329553-93tbgn2b 366 44 Golgi Golgi NNP cord-329553-93tbgn2b 366 45 apparatus apparatus NN cord-329553-93tbgn2b 366 46 sialyltransferase sialyltransferase NN cord-329553-93tbgn2b 366 47 to to IN cord-329553-93tbgn2b 366 48 a a DT cord-329553-93tbgn2b 366 49 secretory secretory JJ cord-329553-93tbgn2b 366 50 protein protein NN cord-329553-93tbgn2b 366 51 by by IN cord-329553-93tbgn2b 366 52 replacement replacement NN cord-329553-93tbgn2b 366 53 of of IN cord-329553-93tbgn2b 366 54 the the DT cord-329553-93tbgn2b 366 55 NH NH NNP cord-329553-93tbgn2b 366 56 2-terminal 2-terminal CD cord-329553-93tbgn2b 366 57 signal signal NN cord-329553-93tbgn2b 366 58 anchor anchor NN cord-329553-93tbgn2b 366 59 with with IN cord-329553-93tbgn2b 366 60 a a DT cord-329553-93tbgn2b 366 61 signal signal NN cord-329553-93tbgn2b 366 62 peptide peptide NN cord-329553-93tbgn2b 366 63 Cloning cloning NN cord-329553-93tbgn2b 366 64 of of IN cord-329553-93tbgn2b 366 65 cDNA cdna NN cord-329553-93tbgn2b 366 66 encoding encoding NN cord-329553-93tbgn2b 367 1 the the DT cord-329553-93tbgn2b 367 2 membrane membrane NN cord-329553-93tbgn2b 367 3 - - HYPH cord-329553-93tbgn2b 367 4 bound bind VBN cord-329553-93tbgn2b 367 5 form form NN cord-329553-93tbgn2b 367 6 of of IN cord-329553-93tbgn2b 367 7 bovine bovine JJ cord-329553-93tbgn2b 367 8 01,4-galactosyltransferase 01,4-galactosyltransferase CD cord-329553-93tbgn2b 367 9 Mannose-6-phosphate mannose-6-phosphate NN cord-329553-93tbgn2b 367 10 receptors receptor NNS cord-329553-93tbgn2b 367 11 and and CC cord-329553-93tbgn2b 367 12 lysosomal lysosomal JJ cord-329553-93tbgn2b 367 13 enzyme enzyme NN cord-329553-93tbgn2b 367 14 targeting target VBG cord-329553-93tbgn2b 367 15 Involvement Involvement NNP cord-329553-93tbgn2b 367 16 of of IN cord-329553-93tbgn2b 367 17 0-COP 0-cop CD cord-329553-93tbgn2b 367 18 in in IN cord-329553-93tbgn2b 367 19 membrane membrane NN cord-329553-93tbgn2b 367 20 traffic traffic NN cord-329553-93tbgn2b 367 21 through through IN cord-329553-93tbgn2b 367 22 the the DT cord-329553-93tbgn2b 367 23 Golgi Golgi NNP cord-329553-93tbgn2b 367 24 complex complex JJ cord-329553-93tbgn2b 367 25 Attachment attachment NN cord-329553-93tbgn2b 367 26 of of IN cord-329553-93tbgn2b 367 27 terminal terminal NNP cord-329553-93tbgn2b 367 28 N n CD cord-329553-93tbgn2b 367 29 - - HYPH cord-329553-93tbgn2b 367 30 acetylglucosamine acetylglucosamine NNP cord-329553-93tbgn2b 367 31 to to IN cord-329553-93tbgn2b 367 32 asparagine asparagine NN cord-329553-93tbgn2b 367 33 - - HYPH cord-329553-93tbgn2b 367 34 linked link VBN cord-329553-93tbgn2b 367 35 oligosaccharides oligosaccharide NNS cord-329553-93tbgn2b 367 36 occurs occur VBZ cord-329553-93tbgn2b 367 37 in in IN cord-329553-93tbgn2b 367 38 central central JJ cord-329553-93tbgn2b 367 39 cistemae cistemae NN cord-329553-93tbgn2b 367 40 of of IN cord-329553-93tbgn2b 367 41 the the DT cord-329553-93tbgn2b 367 42 Golgi Golgi NNP cord-329553-93tbgn2b 367 43 stack stack VB cord-329553-93tbgn2b 367 44 The the DT cord-329553-93tbgn2b 367 45 Golgi Golgi NNP cord-329553-93tbgn2b 367 46 apparatus apparatus NN cord-329553-93tbgn2b 367 47 ( ( -LRB- cord-329553-93tbgn2b 367 48 complex)-(1954 complex)-(1954 NN cord-329553-93tbgn2b 367 49 - - HYPH cord-329553-93tbgn2b 367 50 1981 1981 CD cord-329553-93tbgn2b 367 51 ) ) -RRB- cord-329553-93tbgn2b 368 1 -from -from JJ cord-329553-93tbgn2b 368 2 artifact artifact NN cord-329553-93tbgn2b 368 3 to to IN cord-329553-93tbgn2b 368 4 center center JJ cord-329553-93tbgn2b 368 5 stage stage NN cord-329553-93tbgn2b 369 1 Endocytotic endocytotic JJ cord-329553-93tbgn2b 369 2 membrane membrane NN cord-329553-93tbgn2b 369 3 traffic traffic NN cord-329553-93tbgn2b 369 4 to to IN cord-329553-93tbgn2b 369 5 the the DT cord-329553-93tbgn2b 369 6 Golgi Golgi NNP cord-329553-93tbgn2b 369 7 apparatus apparatus NN cord-329553-93tbgn2b 369 8 in in IN cord-329553-93tbgn2b 369 9 a a DT cord-329553-93tbgn2b 369 10 regulated regulated JJ cord-329553-93tbgn2b 369 11 secretory secretory JJ cord-329553-93tbgn2b 369 12 cell cell NN cord-329553-93tbgn2b 369 13 line line NN cord-329553-93tbgn2b 370 1 The the DT cord-329553-93tbgn2b 370 2 trams tram NNS cord-329553-93tbgn2b 370 3 - - HYPH cord-329553-93tbgn2b 370 4 Golgi golgi NN cord-329553-93tbgn2b 370 5 network network NN cord-329553-93tbgn2b 370 6 : : : cord-329553-93tbgn2b 370 7 sorting sort VBG cord-329553-93tbgn2b 370 8 at at IN cord-329553-93tbgn2b 370 9 the the DT cord-329553-93tbgn2b 370 10 exit exit NN cord-329553-93tbgn2b 370 11 site site NN cord-329553-93tbgn2b 370 12 of of IN cord-329553-93tbgn2b 370 13 the the DT cord-329553-93tbgn2b 370 14 Golgi Golgi NNP cord-329553-93tbgn2b 370 15 complex complex JJ cord-329553-93tbgn2b 370 16 Complete complete JJ cord-329553-93tbgn2b 370 17 cDNA cdna NN cord-329553-93tbgn2b 370 18 sequence sequence NN cord-329553-93tbgn2b 370 19 encoding encode VBG cord-329553-93tbgn2b 370 20 human human NN cord-329553-93tbgn2b 370 21 0-galactoside 0-galactoside CD cord-329553-93tbgn2b 370 22 a-2,6-sialyltransferase a-2,6-sialyltransferase JJ cord-329553-93tbgn2b 370 23 Structural structural JJ cord-329553-93tbgn2b 370 24 characterization characterization NN cord-329553-93tbgn2b 370 25 of of IN cord-329553-93tbgn2b 370 26 glycoprotein glycoprotein NN cord-329553-93tbgn2b 370 27 carbohydrate carbohydrate NN cord-329553-93tbgn2b 370 28 chains chain NNS cord-329553-93tbgn2b 370 29 by by IN cord-329553-93tbgn2b 370 30 using use VBG cord-329553-93tbgn2b 370 31 digoxigenin digoxigenin NNP cord-329553-93tbgn2b 370 32 - - HYPH cord-329553-93tbgn2b 370 33 labeled label VBN cord-329553-93tbgn2b 370 34 lectins lectin NNS cord-329553-93tbgn2b 370 35 on on IN cord-329553-93tbgn2b 370 36 blots blot NNS cord-329553-93tbgn2b 370 37 Membrane membrane NN cord-329553-93tbgn2b 370 38 orientation orientation NN cord-329553-93tbgn2b 370 39 of of IN cord-329553-93tbgn2b 370 40 rat rat NN cord-329553-93tbgn2b 370 41 gp110 gp110 NNP cord-329553-93tbgn2b 370 42 as as IN cord-329553-93tbgn2b 370 43 studied study VBN cord-329553-93tbgn2b 370 44 by by IN cord-329553-93tbgn2b 370 45 in in FW cord-329553-93tbgn2b 370 46 vitro vitro FW cord-329553-93tbgn2b 370 47 translation translation NN cord-329553-93tbgn2b 371 1 Molecular molecular JJ cord-329553-93tbgn2b 371 2 dissection dissection NN cord-329553-93tbgn2b 371 3 of of IN cord-329553-93tbgn2b 371 4 the the DT cord-329553-93tbgn2b 371 5 NH2 NH2 NNP cord-329553-93tbgn2b 371 6 -terminal -terminal JJ cord-329553-93tbgn2b 371 7 signal signal NNP cord-329553-93tbgn2b 371 8 / / SYM cord-329553-93tbgn2b 371 9 anchor anchor NN cord-329553-93tbgn2b 371 10 sequence sequence NN cord-329553-93tbgn2b 371 11 of of IN cord-329553-93tbgn2b 371 12 rat rat NN cord-329553-93tbgn2b 371 13 dipeptidyl dipeptidyl JJ cord-329553-93tbgn2b 371 14 peptidase peptidase NN cord-329553-93tbgn2b 371 15 IV IV NNP cord-329553-93tbgn2b 371 16 Expression expression NN cord-329553-93tbgn2b 371 17 of of IN cord-329553-93tbgn2b 371 18 enzymatically enzymatically RB cord-329553-93tbgn2b 371 19 active active JJ cord-329553-93tbgn2b 371 20 rat rat NN cord-329553-93tbgn2b 371 21 dipeptidyl dipeptidyl JJ cord-329553-93tbgn2b 371 22 peptidase peptidase NN cord-329553-93tbgn2b 371 23 IV IV NNP cord-329553-93tbgn2b 371 24 in in IN cord-329553-93tbgn2b 371 25 Chinese chinese JJ cord-329553-93tbgn2b 371 26 hamster hamster NN cord-329553-93tbgn2b 371 27 ovary ovary NN cord-329553-93tbgn2b 371 28 cells cell NNS cord-329553-93tbgn2b 371 29 after after IN cord-329553-93tbgn2b 371 30 transfection transfection NN cord-329553-93tbgn2b 372 1 A a DT cord-329553-93tbgn2b 372 2 recycling recycling NN cord-329553-93tbgn2b 372 3 pathway pathway NN cord-329553-93tbgn2b 372 4 between between IN cord-329553-93tbgn2b 372 5 the the DT cord-329553-93tbgn2b 372 6 endoplasmic endoplasmic NN cord-329553-93tbgn2b 372 7 reticulum reticulum NN cord-329553-93tbgn2b 372 8 and and CC cord-329553-93tbgn2b 372 9 the the DT cord-329553-93tbgn2b 372 10 Golgi Golgi NNP cord-329553-93tbgn2b 372 11 apparatus apparatus NN cord-329553-93tbgn2b 372 12 for for IN cord-329553-93tbgn2b 372 13 retention retention NN cord-329553-93tbgn2b 372 14 of of IN cord-329553-93tbgn2b 372 15 unassembled unassembled JJ cord-329553-93tbgn2b 372 16 MHC MHC NNP cord-329553-93tbgn2b 372 17 class class NN cord-329553-93tbgn2b 373 1 I -PRON- PRP cord-329553-93tbgn2b 373 2 molecules molecule VBZ cord-329553-93tbgn2b 373 3 Sorting sort VBG cord-329553-93tbgn2b 373 4 and and CC cord-329553-93tbgn2b 373 5 traffic traffic NN cord-329553-93tbgn2b 373 6 in in IN cord-329553-93tbgn2b 373 7 the the DT cord-329553-93tbgn2b 373 8 central central JJ cord-329553-93tbgn2b 373 9 vacuolar vacuolar NNP cord-329553-93tbgn2b 373 10 system system NN cord-329553-93tbgn2b 373 11 Assembly Assembly NNP cord-329553-93tbgn2b 373 12 of of IN cord-329553-93tbgn2b 373 13 asparagine asparagine NN cord-329553-93tbgn2b 373 14 - - HYPH cord-329553-93tbgn2b 373 15 linked link VBN cord-329553-93tbgn2b 373 16 oligosaccharides oligosaccharide NNS cord-329553-93tbgn2b 374 1 The the DT cord-329553-93tbgn2b 374 2 biogenesis biogenesis NN cord-329553-93tbgn2b 374 3 of of IN cord-329553-93tbgn2b 374 4 lysosomes lysosome NNS cord-329553-93tbgn2b 374 5 Cloning cloning NN cord-329553-93tbgn2b 374 6 and and CC cord-329553-93tbgn2b 374 7 expression expression NN cord-329553-93tbgn2b 374 8 of of IN cord-329553-93tbgn2b 374 9 N n NN cord-329553-93tbgn2b 374 10 - - NN cord-329553-93tbgn2b 374 11 acetylglucosaminyltransferase acetylglucosaminyltransferase NNP cord-329553-93tbgn2b 374 12 I -PRON- PRP cord-329553-93tbgn2b 374 13 , , , cord-329553-93tbgn2b 374 14 the the DT cord-329553-93tbgn2b 374 15 medial medial JJ cord-329553-93tbgn2b 374 16 Golgi Golgi NNP cord-329553-93tbgn2b 374 17 transferase transferase NN cord-329553-93tbgn2b 374 18 that that WDT cord-329553-93tbgn2b 374 19 initiates initiate VBZ cord-329553-93tbgn2b 374 20 complex complex JJ cord-329553-93tbgn2b 374 21 N n CD cord-329553-93tbgn2b 374 22 - - HYPH cord-329553-93tbgn2b 374 23 linked link VBN cord-329553-93tbgn2b 374 24 carbohydrate carbohydrate NN cord-329553-93tbgn2b 374 25 formation formation NN cord-329553-93tbgn2b 375 1 The the DT cord-329553-93tbgn2b 375 2 role role NN cord-329553-93tbgn2b 375 3 of of IN cord-329553-93tbgn2b 375 4 a a DT cord-329553-93tbgn2b 375 5 cathepsin cathepsin NN cord-329553-93tbgn2b 375 6 D d NN cord-329553-93tbgn2b 375 7 - - HYPH cord-329553-93tbgn2b 375 8 like like JJ cord-329553-93tbgn2b 375 9 activity activity NN cord-329553-93tbgn2b 375 10 in in IN cord-329553-93tbgn2b 375 11 the the DT cord-329553-93tbgn2b 375 12 release release NN cord-329553-93tbgn2b 375 13 of of IN cord-329553-93tbgn2b 375 14 Gal Gal NNP cord-329553-93tbgn2b 375 15 01 01 CD cord-329553-93tbgn2b 375 16 - - HYPH cord-329553-93tbgn2b 375 17 4GlcNac 4glcnac CD cord-329553-93tbgn2b 375 18 a2 a2 NN cord-329553-93tbgn2b 375 19 - - HYPH cord-329553-93tbgn2b 375 20 6sialyltransferase 6sialyltransferase CD cord-329553-93tbgn2b 375 21 from from IN cord-329553-93tbgn2b 375 22 rat rat NN cord-329553-93tbgn2b 375 23 liver liver NN cord-329553-93tbgn2b 375 24 Golgi Golgi NNP cord-329553-93tbgn2b 375 25 membranes membrane NNS cord-329553-93tbgn2b 375 26 during during IN cord-329553-93tbgn2b 375 27 the the DT cord-329553-93tbgn2b 375 28 acute acute JJ cord-329553-93tbgn2b 375 29 - - HYPH cord-329553-93tbgn2b 375 30 phase phase NN cord-329553-93tbgn2b 375 31 response response NN cord-329553-93tbgn2b 375 32 Studies study NNS cord-329553-93tbgn2b 375 33 on on IN cord-329553-93tbgn2b 375 34 the the DT cord-329553-93tbgn2b 375 35 effect effect NN cord-329553-93tbgn2b 375 36 of of IN cord-329553-93tbgn2b 375 37 lysosomotropic lysosomotropic JJ cord-329553-93tbgn2b 375 38 agents agent NNS cord-329553-93tbgn2b 375 39 on on IN cord-329553-93tbgn2b 375 40 the the DT cord-329553-93tbgn2b 375 41 release release NN cord-329553-93tbgn2b 375 42 of of IN cord-329553-93tbgn2b 375 43 Gal Gal NNP cord-329553-93tbgn2b 375 44 01 01 CD cord-329553-93tbgn2b 375 45 - - HYPH cord-329553-93tbgn2b 375 46 4G1cNac 4g1cnac CD cord-329553-93tbgn2b 375 47 a2 a2 NN cord-329553-93tbgn2b 375 48 - - HYPH cord-329553-93tbgn2b 375 49 6sialyltransferase 6sialyltransferase CD cord-329553-93tbgn2b 375 50 from from IN cord-329553-93tbgn2b 375 51 rat rat NN cord-329553-93tbgn2b 375 52 liver liver NN cord-329553-93tbgn2b 375 53 slices slice NNS cord-329553-93tbgn2b 375 54 during during IN cord-329553-93tbgn2b 375 55 the the DT cord-329553-93tbgn2b 375 56 acute acute JJ cord-329553-93tbgn2b 375 57 - - HYPH cord-329553-93tbgn2b 375 58 phase phase NN cord-329553-93tbgn2b 375 59 response response NN cord-329553-93tbgn2b 375 60 Biogenetic Biogenetic NNP cord-329553-93tbgn2b 375 61 pathways pathway NNS cord-329553-93tbgn2b 375 62 ofplasma ofplasma VBP cord-329553-93tbgn2b 375 63 membrane membrane NN cord-329553-93tbgn2b 375 64 proteins protein NNS cord-329553-93tbgn2b 375 65 in in IN cord-329553-93tbgn2b 375 66 Caco-2 Caco-2 NNP cord-329553-93tbgn2b 375 67 , , , cord-329553-93tbgn2b 375 68 a a DT cord-329553-93tbgn2b 375 69 human human JJ cord-329553-93tbgn2b 375 70 intestinal intestinal JJ cord-329553-93tbgn2b 375 71 epithelial epithelial JJ cord-329553-93tbgn2b 375 72 cell cell NN cord-329553-93tbgn2b 375 73 line line NN cord-329553-93tbgn2b 375 74 Vectorial Vectorial NNP cord-329553-93tbgn2b 375 75 targeting targeting NN cord-329553-93tbgn2b 375 76 of of IN cord-329553-93tbgn2b 375 77 an an DT cord-329553-93tbgn2b 375 78 endogenous endogenous JJ cord-329553-93tbgn2b 375 79 apical apical JJ cord-329553-93tbgn2b 375 80 membrane membrane NN cord-329553-93tbgn2b 375 81 sialoglycoprotein sialoglycoprotein NNP cord-329553-93tbgn2b 375 82 and and CC cord-329553-93tbgn2b 375 83 uvomorulin uvomorulin NNP cord-329553-93tbgn2b 375 84 in in IN cord-329553-93tbgn2b 375 85 MDCK MDCK NNP cord-329553-93tbgn2b 375 86 cells cell NNS cord-329553-93tbgn2b 376 1 A a DT cord-329553-93tbgn2b 376 2 human human JJ cord-329553-93tbgn2b 376 3 homologue homologue NN cord-329553-93tbgn2b 376 4 of of IN cord-329553-93tbgn2b 376 5 the the DT cord-329553-93tbgn2b 376 6 yeast yeast NN cord-329553-93tbgn2b 376 7 HDEL HDEL NNP cord-329553-93tbgn2b 376 8 receptor receptor NN cord-329553-93tbgn2b 377 1 A a DT cord-329553-93tbgn2b 377 2 vital vital JJ cord-329553-93tbgn2b 377 3 stain stain NN cord-329553-93tbgn2b 377 4 for for IN cord-329553-93tbgn2b 377 5 the the DT cord-329553-93tbgn2b 377 6 Golgi Golgi NNP cord-329553-93tbgn2b 377 7 apparatus apparatus NN cord-329553-93tbgn2b 377 8 Lectins Lectins NNP cord-329553-93tbgn2b 377 9 as as IN cord-329553-93tbgn2b 377 10 molecules molecule NNS cord-329553-93tbgn2b 377 11 and and CC cord-329553-93tbgn2b 377 12 as as IN cord-329553-93tbgn2b 377 13 tools tool NNS cord-329553-93tbgn2b 377 14 A a DT cord-329553-93tbgn2b 377 15 Glycophospholipid Glycophospholipid NNP cord-329553-93tbgn2b 377 16 membrane membrane NN cord-329553-93tbgn2b 377 17 anchor anchor NN cord-329553-93tbgn2b 377 18 acts act NNS cord-329553-93tbgn2b 377 19 as as IN cord-329553-93tbgn2b 377 20 an an DT cord-329553-93tbgn2b 377 21 apical apical JJ cord-329553-93tbgn2b 377 22 targeting targeting NN cord-329553-93tbgn2b 377 23 signal signal NN cord-329553-93tbgn2b 377 24 in in IN cord-329553-93tbgn2b 377 25 polarized polarized JJ cord-329553-93tbgn2b 377 26 epithelial epithelial JJ cord-329553-93tbgn2b 377 27 cells cell NNS cord-329553-93tbgn2b 377 28 Antibodies antibody NNS cord-329553-93tbgn2b 377 29 to to IN cord-329553-93tbgn2b 377 30 the the DT cord-329553-93tbgn2b 377 31 Golgi Golgi NNP cord-329553-93tbgn2b 377 32 complex complex NN cord-329553-93tbgn2b 377 33 and and CC cord-329553-93tbgn2b 378 1 the the DT cord-329553-93tbgn2b 378 2 rough rough JJ cord-329553-93tbgn2b 378 3 endoplasmic endoplasmic NN cord-329553-93tbgn2b 378 4 reticulum reticulum NN cord-329553-93tbgn2b 378 5 Involvement Involvement NNP cord-329553-93tbgn2b 378 6 of of IN cord-329553-93tbgn2b 378 7 both both DT cord-329553-93tbgn2b 378 8 vectorial vectorial JJ cord-329553-93tbgn2b 378 9 and and CC cord-329553-93tbgn2b 378 10 transcytotic transcytotic JJ cord-329553-93tbgn2b 378 11 pathways pathway NNS cord-329553-93tbgn2b 378 12 in in IN cord-329553-93tbgn2b 378 13 the the DT cord-329553-93tbgn2b 378 14 preferential preferential JJ cord-329553-93tbgn2b 378 15 apical apical JJ cord-329553-93tbgn2b 378 16 cell cell NN cord-329553-93tbgn2b 378 17 surface surface NN cord-329553-93tbgn2b 378 18 localization localization NN cord-329553-93tbgn2b 378 19 of of IN cord-329553-93tbgn2b 378 20 rat rat NN cord-329553-93tbgn2b 378 21 dipeptidyl dipeptidyl JJ cord-329553-93tbgn2b 378 22 peptidase peptidase NN cord-329553-93tbgn2b 378 23 IV IV NNP cord-329553-93tbgn2b 378 24 Apical Apical NNP cord-329553-93tbgn2b 378 25 cell cell NN cord-329553-93tbgn2b 378 26 surface surface NN cord-329553-93tbgn2b 378 27 expression expression NN cord-329553-93tbgn2b 378 28 of of IN cord-329553-93tbgn2b 378 29 rat rat NN cord-329553-93tbgn2b 378 30 dipeptidyl dipeptidyl JJ cord-329553-93tbgn2b 378 31 peptidase peptidase NN cord-329553-93tbgn2b 378 32 IV IV NNP cord-329553-93tbgn2b 378 33 in in IN cord-329553-93tbgn2b 378 34 transfected transfecte VBN cord-329553-93tbgn2b 378 35 Madin Madin NNP cord-329553-93tbgn2b 378 36 - - HYPH cord-329553-93tbgn2b 378 37 Darby Darby NNP cord-329553-93tbgn2b 378 38 canine canine JJ cord-329553-93tbgn2b 378 39 kidney kidney NN cord-329553-93tbgn2b 378 40 cells cell VBZ cord-329553-93tbgn2b 378 41 A a DT cord-329553-93tbgn2b 378 42 specific specific JJ cord-329553-93tbgn2b 378 43 transmembrane transmembrane NN cord-329553-93tbgn2b 378 44 domain domain NN cord-329553-93tbgn2b 378 45 of of IN cord-329553-93tbgn2b 378 46 a a DT cord-329553-93tbgn2b 378 47 coronavirus coronavirus NN cord-329553-93tbgn2b 378 48 El El NNP cord-329553-93tbgn2b 378 49 glycoprotein glycoprotein NN cord-329553-93tbgn2b 378 50 is be VBZ cord-329553-93tbgn2b 378 51 required require VBN cord-329553-93tbgn2b 378 52 for for IN cord-329553-93tbgn2b 378 53 its -PRON- PRP$ cord-329553-93tbgn2b 378 54 retention retention NN cord-329553-93tbgn2b 378 55 in in IN cord-329553-93tbgn2b 378 56 the the DT cord-329553-93tbgn2b 378 57 Golgi Golgi NNP cord-329553-93tbgn2b 378 58 region region NN cord-329553-93tbgn2b 379 1 The the DT cord-329553-93tbgn2b 379 2 El El NNP cord-329553-93tbgn2b 379 3 glycoprotein glycoprotein NN cord-329553-93tbgn2b 379 4 of of IN cord-329553-93tbgn2b 379 5 an an DT cord-329553-93tbgn2b 379 6 avian avian JJ cord-329553-93tbgn2b 379 7 coronavirus coronavirus NN cord-329553-93tbgn2b 379 8 is be VBZ cord-329553-93tbgn2b 379 9 targeted target VBN cord-329553-93tbgn2b 379 10 to to IN cord-329553-93tbgn2b 379 11 the the DT cord-329553-93tbgn2b 379 12 cis cis NNP cord-329553-93tbgn2b 379 13 Golgi Golgi NNP cord-329553-93tbgn2b 379 14 complex complex JJ cord-329553-93tbgn2b 379 15 Identification identification NN cord-329553-93tbgn2b 379 16 of of IN cord-329553-93tbgn2b 379 17 the the DT cord-329553-93tbgn2b 379 18 fulllength fulllength NN cord-329553-93tbgn2b 379 19 coding coding NN cord-329553-93tbgn2b 379 20 sequence sequence NN cord-329553-93tbgn2b 379 21 for for IN cord-329553-93tbgn2b 379 22 human human JJ cord-329553-93tbgn2b 379 23 galactosyltransferase galactosyltransferase NN cord-329553-93tbgn2b 379 24 ( ( -LRB- cord-329553-93tbgn2b 379 25 0-N 0-n CD cord-329553-93tbgn2b 379 26 - - HYPH cord-329553-93tbgn2b 379 27 acetylglucosamine acetylglucosamine NNP cord-329553-93tbgn2b 379 28 : : : cord-329553-93tbgn2b 379 29 01,4-galactosyltransferase 01,4-galactosyltransferase LS cord-329553-93tbgn2b 379 30 ) ) -RRB- cord-329553-93tbgn2b 380 1 Properties property NNS cord-329553-93tbgn2b 380 2 of of IN cord-329553-93tbgn2b 380 3 succinylated succinylated JJ cord-329553-93tbgn2b 380 4 wheat wheat NN cord-329553-93tbgn2b 380 5 germ germ NNP cord-329553-93tbgn2b 380 6 agglutinin agglutinin NNP cord-329553-93tbgn2b 380 7 Sugar sugar NN cord-329553-93tbgn2b 380 8 - - HYPH cord-329553-93tbgn2b 380 9 lectin lectin NN cord-329553-93tbgn2b 380 10 interactions interaction NNS cord-329553-93tbgn2b 380 11 : : : cord-329553-93tbgn2b 381 1 how how WRB cord-329553-93tbgn2b 381 2 does do VBZ cord-329553-93tbgn2b 381 3 wheat wheat NN cord-329553-93tbgn2b 381 4 germ germ NN cord-329553-93tbgn2b 381 5 agglutinin agglutinin NNS cord-329553-93tbgn2b 381 6 bind bind NNP cord-329553-93tbgn2b 381 7 sialoglycoconjugates sialoglycoconjugates NNP cord-329553-93tbgn2b 381 8 Sequences Sequences NNPS cord-329553-93tbgn2b 381 9 within within IN cord-329553-93tbgn2b 381 10 and and CC cord-329553-93tbgn2b 381 11 adjacent adjacent JJ cord-329553-93tbgn2b 381 12 to to IN cord-329553-93tbgn2b 381 13 the the DT cord-329553-93tbgn2b 381 14 transmembrane transmembrane NN cord-329553-93tbgn2b 381 15 segment segment NN cord-329553-93tbgn2b 381 16 of of IN cord-329553-93tbgn2b 381 17 a-2,6-sialyltransferase a-2,6-sialyltransferase NNP cord-329553-93tbgn2b 382 1 specify specify VB cord-329553-93tbgn2b 382 2 Golgi Golgi NNP cord-329553-93tbgn2b 382 3 retention retention NN cord-329553-93tbgn2b 383 1 The the DT cord-329553-93tbgn2b 383 2 membrane membrane NN cord-329553-93tbgn2b 383 3 spanning span VBG cord-329553-93tbgn2b 383 4 domain domain NN cord-329553-93tbgn2b 383 5 of of IN cord-329553-93tbgn2b 383 6 fl-1,4-galactosyltransferase fl-1,4-galactosyltransferase NN cord-329553-93tbgn2b 384 1 specifies specify VBZ cord-329553-93tbgn2b 384 2 trans trans NNP cord-329553-93tbgn2b 384 3 Golgi Golgi NNP cord-329553-93tbgn2b 384 4 localization localization NN cord-329553-93tbgn2b 384 5 Primary primary JJ cord-329553-93tbgn2b 384 6 structure structure NN cord-329553-93tbgn2b 384 7 of of IN cord-329553-93tbgn2b 384 8 rat rat NN cord-329553-93tbgn2b 384 9 liver liver NN cord-329553-93tbgn2b 384 10 dipeptidyl dipeptidyl NNP cord-329553-93tbgn2b 384 11 peptidase peptidase NN cord-329553-93tbgn2b 384 12 IV IV NNP cord-329553-93tbgn2b 384 13 deduced deduce VBN cord-329553-93tbgn2b 384 14 from from IN cord-329553-93tbgn2b 384 15 its -PRON- PRP$ cord-329553-93tbgn2b 384 16 cDNA cdna NN cord-329553-93tbgn2b 384 17 and and CC cord-329553-93tbgn2b 384 18 identification identification NN cord-329553-93tbgn2b 384 19 of of IN cord-329553-93tbgn2b 384 20 the the DT cord-329553-93tbgn2b 384 21 NH2-terminal nh2-terminal JJ cord-329553-93tbgn2b 384 22 signal signal NN cord-329553-93tbgn2b 384 23 sequence sequence NN cord-329553-93tbgn2b 384 24 as as IN cord-329553-93tbgn2b 384 25 the the DT cord-329553-93tbgn2b 384 26 membrane membrane NN cord-329553-93tbgn2b 384 27 - - HYPH cord-329553-93tbgn2b 384 28 anchoring anchor VBG cord-329553-93tbgn2b 384 29 domain domain NN cord-329553-93tbgn2b 385 1 The the DT cord-329553-93tbgn2b 385 2 trans trans NN cord-329553-93tbgn2b 385 3 - - JJ cord-329553-93tbgn2b 385 4 most most JJS cord-329553-93tbgn2b 385 5 cistemae cistemae NN cord-329553-93tbgn2b 385 6 of of IN cord-329553-93tbgn2b 385 7 the the DT cord-329553-93tbgn2b 385 8 Golgi Golgi NNP cord-329553-93tbgn2b 385 9 complex complex NN cord-329553-93tbgn2b 385 10 : : : cord-329553-93tbgn2b 385 11 a a DT cord-329553-93tbgn2b 385 12 compartment compartment NN cord-329553-93tbgn2b 385 13 for for IN cord-329553-93tbgn2b 385 14 sorting sorting NN cord-329553-93tbgn2b 385 15 of of IN cord-329553-93tbgn2b 385 16 secretory secretory NN cord-329553-93tbgn2b 385 17 and and CC cord-329553-93tbgn2b 385 18 plasma plasma NN cord-329553-93tbgn2b 385 19 membrane membrane NN cord-329553-93tbgn2b 385 20 proteins protein NNS cord-329553-93tbgn2b 385 21 Glycosyltransferase Glycosyltransferase NNP cord-329553-93tbgn2b 385 22 : : : cord-329553-93tbgn2b 386 1 structure structure NN cord-329553-93tbgn2b 386 2 , , , cord-329553-93tbgn2b 386 3 localization localization NN cord-329553-93tbgn2b 386 4 , , , cord-329553-93tbgn2b 386 5 and and CC cord-329553-93tbgn2b 386 6 control control NN cord-329553-93tbgn2b 386 7 of of IN cord-329553-93tbgn2b 386 8 cell cell NN cord-329553-93tbgn2b 386 9 type type NN cord-329553-93tbgn2b 386 10 - - HYPH cord-329553-93tbgn2b 386 11 specific specific JJ cord-329553-93tbgn2b 386 12 glycosylation glycosylation NN cord-329553-93tbgn2b 386 13 Evidence evidence NN cord-329553-93tbgn2b 386 14 that that IN cord-329553-93tbgn2b 386 15 luminal luminal JJ cord-329553-93tbgn2b 386 16 ER er NN cord-329553-93tbgn2b 386 17 proteins protein NNS cord-329553-93tbgn2b 386 18 are be VBP cord-329553-93tbgn2b 386 19 sorted sort VBN cord-329553-93tbgn2b 386 20 from from IN cord-329553-93tbgn2b 386 21 secreted secreted JJ cord-329553-93tbgn2b 386 22 proteins protein NNS cord-329553-93tbgn2b 386 23 in in IN cord-329553-93tbgn2b 386 24 a a DT cord-329553-93tbgn2b 386 25 post post JJ cord-329553-93tbgn2b 386 26 - - JJ cord-329553-93tbgn2b 386 27 ER er JJ cord-329553-93tbgn2b 386 28 compartment compartment NN cord-329553-93tbgn2b 386 29 Recycling recycling NN cord-329553-93tbgn2b 386 30 of of IN cord-329553-93tbgn2b 386 31 proteins protein NNS cord-329553-93tbgn2b 386 32 between between IN cord-329553-93tbgn2b 386 33 the the DT cord-329553-93tbgn2b 386 34 endoplasmic endoplasmic NN cord-329553-93tbgn2b 386 35 reticulum reticulum NN cord-329553-93tbgn2b 386 36 and and CC cord-329553-93tbgn2b 386 37 Golgi Golgi NNP cord-329553-93tbgn2b 386 38 complex complex JJ cord-329553-93tbgn2b 386 39 Biosynthetic biosynthetic JJ cord-329553-93tbgn2b 386 40 protein protein NN cord-329553-93tbgn2b 386 41 transport transport NN cord-329553-93tbgn2b 386 42 and and CC cord-329553-93tbgn2b 386 43 sorting sorting NN cord-329553-93tbgn2b 386 44 by by IN cord-329553-93tbgn2b 386 45 the the DT cord-329553-93tbgn2b 386 46 endoplasmic endoplasmic NN cord-329553-93tbgn2b 386 47 reticulum reticulum NN cord-329553-93tbgn2b 386 48 and and CC cord-329553-93tbgn2b 386 49 Golgi Golgi NNP cord-329553-93tbgn2b 386 50 Morphogenesis Morphogenesis NNP cord-329553-93tbgn2b 386 51 of of IN cord-329553-93tbgn2b 386 52 the the DT cord-329553-93tbgn2b 386 53 polarized polarized JJ cord-329553-93tbgn2b 386 54 epithelial epithelial JJ cord-329553-93tbgn2b 386 55 cell cell NN cord-329553-93tbgn2b 386 56 phenotype phenotype NN cord-329553-93tbgn2b 386 57 Regulation regulation NN cord-329553-93tbgn2b 386 58 of of IN cord-329553-93tbgn2b 386 59 protein protein NN cord-329553-93tbgn2b 386 60 export export NN cord-329553-93tbgn2b 386 61 from from IN cord-329553-93tbgn2b 386 62 the the DT cord-329553-93tbgn2b 386 63 endoplasmic endoplasmic NN cord-329553-93tbgn2b 386 64 reticulum reticulum NN cord-329553-93tbgn2b 386 65 Immunocytochemical Immunocytochemical NNP cord-329553-93tbgn2b 386 66 localization localization NN cord-329553-93tbgn2b 386 67 of of IN cord-329553-93tbgn2b 386 68 galactosyltransferase galactosyltransferase NN cord-329553-93tbgn2b 386 69 in in IN cord-329553-93tbgn2b 386 70 Hela Hela NNP cord-329553-93tbgn2b 386 71 cells cell NNS cord-329553-93tbgn2b 386 72 : : : cord-329553-93tbgn2b 386 73 codistribution codistribution NN cord-329553-93tbgn2b 386 74 with with IN cord-329553-93tbgn2b 386 75 thiamine thiamine NN cord-329553-93tbgn2b 386 76 pyrophosphatase pyrophosphatase NN cord-329553-93tbgn2b 386 77 in in IN cord-329553-93tbgn2b 386 78 trans trans NNP cord-329553-93tbgn2b 386 79 - - NNP cord-329553-93tbgn2b 386 80 Golgi Golgi NNP cord-329553-93tbgn2b 386 81 cisternae cisternae NNP cord-329553-93tbgn2b 386 82 Demonstration Demonstration NNP cord-329553-93tbgn2b 386 83 of of IN cord-329553-93tbgn2b 386 84 an an DT cord-329553-93tbgn2b 386 85 extensive extensive JJ cord-329553-93tbgn2b 386 86 trans trans JJ cord-329553-93tbgn2b 386 87 - - JJ cord-329553-93tbgn2b 386 88 tubular tubular JJ cord-329553-93tbgn2b 386 89 network network NN cord-329553-93tbgn2b 386 90 continuous continuous JJ cord-329553-93tbgn2b 386 91 with with IN cord-329553-93tbgn2b 386 92 the the DT cord-329553-93tbgn2b 386 93 Golgi Golgi NNP cord-329553-93tbgn2b 386 94 apparatus apparatus NN cord-329553-93tbgn2b 386 95 that that WDT cord-329553-93tbgn2b 386 96 may may MD cord-329553-93tbgn2b 386 97 function function VB cord-329553-93tbgn2b 386 98 in in IN cord-329553-93tbgn2b 386 99 glycosylation glycosylation NN cord-329553-93tbgn2b 386 100 Bovine bovine NN cord-329553-93tbgn2b 386 101 ßl-4-galactosyltransferase ßl-4-galactosyltransferase NNP cord-329553-93tbgn2b 386 102 : : : cord-329553-93tbgn2b 387 1 two two CD cord-329553-93tbgn2b 387 2 sets set NNS cord-329553-93tbgn2b 387 3 of of IN cord-329553-93tbgn2b 387 4 mRNA mrna NN cord-329553-93tbgn2b 387 5 transcripts transcript NNS cord-329553-93tbgn2b 387 6 encode encode VBP cord-329553-93tbgn2b 387 7 two two CD cord-329553-93tbgn2b 387 8 forms form NNS cord-329553-93tbgn2b 387 9 of of IN cord-329553-93tbgn2b 387 10 the the DT cord-329553-93tbgn2b 387 11 protein protein NN cord-329553-93tbgn2b 387 12 with with IN cord-329553-93tbgn2b 387 13 different different JJ cord-329553-93tbgn2b 387 14 amino amino NN cord-329553-93tbgn2b 387 15 - - HYPH cord-329553-93tbgn2b 387 16 terminal terminal NN cord-329553-93tbgn2b 387 17 domains domain NNS cord-329553-93tbgn2b 387 18 : : : cord-329553-93tbgn2b 387 19 in in IN cord-329553-93tbgn2b 387 20 vitro vitro FW cord-329553-93tbgn2b 387 21 translation translation NN cord-329553-93tbgn2b 387 22 experiments experiment NNS cord-329553-93tbgn2b 387 23 demonstrate demonstrate VBP cord-329553-93tbgn2b 387 24 that that IN cord-329553-93tbgn2b 387 25 both both PDT cord-329553-93tbgn2b 387 26 the the DT cord-329553-93tbgn2b 387 27 short short JJ cord-329553-93tbgn2b 387 28 and and CC cord-329553-93tbgn2b 387 29 the the DT cord-329553-93tbgn2b 387 30 long long JJ cord-329553-93tbgn2b 387 31 forms form NNS cord-329553-93tbgn2b 387 32 of of IN cord-329553-93tbgn2b 387 33 the the DT cord-329553-93tbgn2b 387 34 enzyme enzyme NN cord-329553-93tbgn2b 387 35 are be VBP cord-329553-93tbgn2b 387 36 type type NN cord-329553-93tbgn2b 387 37 II II NNP cord-329553-93tbgn2b 387 38 membrane membrane NN cord-329553-93tbgn2b 387 39 - - HYPH cord-329553-93tbgn2b 387 40 bound bind VBN cord-329553-93tbgn2b 387 41 glycoproteins glycoprotein NNS cord-329553-93tbgn2b 387 42 Antibodies antibody NNS cord-329553-93tbgn2b 387 43 to to IN cord-329553-93tbgn2b 387 44 rat rat NN cord-329553-93tbgn2b 387 45 pancreas pancreas NN cord-329553-93tbgn2b 387 46 Golgi Golgi NNP cord-329553-93tbgn2b 387 47 subfractions subfraction NNS cord-329553-93tbgn2b 387 48 : : : cord-329553-93tbgn2b 387 49 identification identification NN cord-329553-93tbgn2b 387 50 of of IN cord-329553-93tbgn2b 387 51 a a DT cord-329553-93tbgn2b 387 52 58-kD 58-kd CD cord-329553-93tbgn2b 387 53 cis cis NNP cord-329553-93tbgn2b 387 54 - - HYPH cord-329553-93tbgn2b 387 55 Golgi Golgi NNP cord-329553-93tbgn2b 387 56 protein protein NN cord-329553-93tbgn2b 387 57 Molecular molecular JJ cord-329553-93tbgn2b 387 58 cloning cloning NN cord-329553-93tbgn2b 387 59 and and CC cord-329553-93tbgn2b 387 60 expression expression NN cord-329553-93tbgn2b 387 61 of of IN cord-329553-93tbgn2b 387 62 cDNA cdna NN cord-329553-93tbgn2b 387 63 encoding encode VBG cord-329553-93tbgn2b 387 64 the the DT cord-329553-93tbgn2b 387 65 enzyme enzyme NN cord-329553-93tbgn2b 387 66 that that WDT cord-329553-93tbgn2b 387 67 controls control VBZ cord-329553-93tbgn2b 387 68 conversion conversion NN cord-329553-93tbgn2b 387 69 of of IN cord-329553-93tbgn2b 387 70 high high JJ cord-329553-93tbgn2b 387 71 - - HYPH cord-329553-93tbgn2b 387 72 mannose mannose NN cord-329553-93tbgn2b 387 73 to to IN cord-329553-93tbgn2b 387 74 hybrid hybrid VB cord-329553-93tbgn2b 387 75 and and CC cord-329553-93tbgn2b 387 76 complex complex JJ cord-329553-93tbgn2b 387 77 N n CD cord-329553-93tbgn2b 387 78 - - HYPH cord-329553-93tbgn2b 387 79 glycans glycan NNS cord-329553-93tbgn2b 387 80 : : : cord-329553-93tbgn2b 388 1 UDP UDP NNP cord-329553-93tbgn2b 388 2 - - HYPH cord-329553-93tbgn2b 388 3 N N NNP cord-329553-93tbgn2b 388 4 - - HYPH cord-329553-93tbgn2b 388 5 acetylglucosamine acetylglucosamine NNP cord-329553-93tbgn2b 389 1 : : : cord-329553-93tbgn2b 389 2 a-3-D a-3-d CD cord-329553-93tbgn2b 389 3 - - HYPH cord-329553-93tbgn2b 389 4 mannoside mannoside NN cord-329553-93tbgn2b 390 1 ß-1,2-Nacetylglucosaminyltransferase ß-1,2-nacetylglucosaminyltransferase UH cord-329553-93tbgn2b 390 2 I -PRON- PRP cord-329553-93tbgn2b 390 3 of of IN cord-329553-93tbgn2b 390 4 53-kD 53-kd CD cord-329553-93tbgn2b 390 5 protein protein NN cord-329553-93tbgn2b 390 6 associated associate VBN cord-329553-93tbgn2b 390 7 with with IN cord-329553-93tbgn2b 390 8 a a DT cord-329553-93tbgn2b 390 9 tubulovesicular tubulovesicular NNP cord-329553-93tbgn2b 390 10 compartment compartment NN cord-329553-93tbgn2b 390 11 at at IN cord-329553-93tbgn2b 390 12 the the DT cord-329553-93tbgn2b 390 13 cis cis NNP cord-329553-93tbgn2b 390 14 - - HYPH cord-329553-93tbgn2b 390 15 side side NN cord-329553-93tbgn2b 390 16 of of IN cord-329553-93tbgn2b 390 17 the the DT cord-329553-93tbgn2b 390 18 Golgi Golgi NNP cord-329553-93tbgn2b 390 19 apparatus apparatus NN cord-329553-93tbgn2b 390 20 Characterization characterization NN cord-329553-93tbgn2b 390 21 of of IN cord-329553-93tbgn2b 390 22 the the DT cord-329553-93tbgn2b 390 23 full full JJ cord-329553-93tbgn2b 390 24 length length NN cord-329553-93tbgn2b 390 25 cDNA cdna NN cord-329553-93tbgn2b 390 26 for for IN cord-329553-93tbgn2b 390 27 murine murine JJ cord-329553-93tbgn2b 390 28 ß-1,4-galactosyltransferase ß-1,4-galactosyltransferase NNP cord-329553-93tbgn2b 390 29 : : : cord-329553-93tbgn2b 391 1 novel novel NN cord-329553-93tbgn2b 391 2 features feature NNS cord-329553-93tbgn2b 391 3 at at IN cord-329553-93tbgn2b 391 4 the the DT cord-329553-93tbgn2b 391 5 Y Y NNP cord-329553-93tbgn2b 391 6 - - HYPH cord-329553-93tbgn2b 391 7 end end NN cord-329553-93tbgn2b 391 8 predict predict VB cord-329553-93tbgn2b 391 9 two two CD cord-329553-93tbgn2b 391 10 translational translational JJ cord-329553-93tbgn2b 391 11 start start NN cord-329553-93tbgn2b 391 12 sites site NNS cord-329553-93tbgn2b 391 13 at at IN cord-329553-93tbgn2b 391 14 two two CD cord-329553-93tbgn2b 391 15 in in IN cord-329553-93tbgn2b 391 16 - - HYPH cord-329553-93tbgn2b 391 17 frame frame NN cord-329553-93tbgn2b 391 18 AUGs aug NNS cord-329553-93tbgn2b 391 19 Polarized polarized JJ cord-329553-93tbgn2b 391 20 sorting sorting NN cord-329553-93tbgn2b 391 21 in in IN cord-329553-93tbgn2b 391 22 epithelia epithelium NNS cord-329553-93tbgn2b 391 23 Intracellular Intracellular NNP cord-329553-93tbgn2b 391 24 movement movement NN cord-329553-93tbgn2b 391 25 of of IN cord-329553-93tbgn2b 391 26 cell cell NN cord-329553-93tbgn2b 391 27 surface surface NN cord-329553-93tbgn2b 391 28 receptors receptor NNS cord-329553-93tbgn2b 391 29 after after IN cord-329553-93tbgn2b 391 30 endocytosis endocytosis NN cord-329553-93tbgn2b 391 31 : : : cord-329553-93tbgn2b 391 32 resialylation resialylation NN cord-329553-93tbgn2b 391 33 of of IN cord-329553-93tbgn2b 391 34 asialo asialo NNP cord-329553-93tbgn2b 391 35 - - HYPH cord-329553-93tbgn2b 391 36 transferrin transferrin NN cord-329553-93tbgn2b 391 37 receptor receptor NN cord-329553-93tbgn2b 391 38 in in IN cord-329553-93tbgn2b 391 39 human human JJ cord-329553-93tbgn2b 391 40 erythroleukemia erythroleukemia NN cord-329553-93tbgn2b 391 41 cells cell VBZ cord-329553-93tbgn2b 391 42 A a DT cord-329553-93tbgn2b 391 43 Golgi Golgi NNP cord-329553-93tbgn2b 391 44 retention retention NN cord-329553-93tbgn2b 391 45 signal signal NN cord-329553-93tbgn2b 391 46 in in IN cord-329553-93tbgn2b 391 47 a a DT cord-329553-93tbgn2b 391 48 membrane membrane NN cord-329553-93tbgn2b 391 49 - - HYPH cord-329553-93tbgn2b 391 50 spamting spamte VBG cord-329553-93tbgn2b 391 51 domain domain NN cord-329553-93tbgn2b 391 52 of of IN cord-329553-93tbgn2b 391 53 coronavirus coronavirus NN cord-329553-93tbgn2b 391 54 El El NNP cord-329553-93tbgn2b 391 55 protein protein NN cord-329553-93tbgn2b 392 1 The the DT cord-329553-93tbgn2b 392 2 transmembrane transmembrane NN cord-329553-93tbgn2b 392 3 domain domain NN cord-329553-93tbgn2b 392 4 of of IN cord-329553-93tbgn2b 392 5 N n CD cord-329553-93tbgn2b 392 6 - - HYPH cord-329553-93tbgn2b 392 7 acetylglucosaminyl acetylglucosaminyl JJ cord-329553-93tbgn2b 392 8 - - HYPH cord-329553-93tbgn2b 392 9 transferase transferase NN cord-329553-93tbgn2b 393 1 I -PRON- PRP cord-329553-93tbgn2b 393 2 contains contain VBZ cord-329553-93tbgn2b 393 3 a a DT cord-329553-93tbgn2b 393 4 Golgi Golgi NNP cord-329553-93tbgn2b 393 5 retention retention NN cord-329553-93tbgn2b 393 6 signal signal NN cord-329553-93tbgn2b 393 7 Lectin Lectin NNP cord-329553-93tbgn2b 393 8 - - HYPH cord-329553-93tbgn2b 393 9 binding bind VBG cord-329553-93tbgn2b 393 10 sites site NNS cord-329553-93tbgn2b 393 11 as as IN cord-329553-93tbgn2b 393 12 markers marker NNS cord-329553-93tbgn2b 393 13 of of IN cord-329553-93tbgn2b 393 14 Golgi Golgi NNP cord-329553-93tbgn2b 393 15 subcompartments subcompartment NNS cord-329553-93tbgn2b 393 16 : : : cord-329553-93tbgn2b 393 17 proximal proximal JJ cord-329553-93tbgn2b 393 18 - - HYPH cord-329553-93tbgn2b 393 19 to to IN cord-329553-93tbgn2b 393 20 - - HYPH cord-329553-93tbgn2b 393 21 distal distal JJ cord-329553-93tbgn2b 393 22 maturation maturation NN cord-329553-93tbgn2b 393 23 of of IN cord-329553-93tbgn2b 393 24 oligosaccharides oligosaccharide NNS cord-329553-93tbgn2b 393 25 Sorting sorting NN cord-329553-93tbgn2b 393 26 of of IN cord-329553-93tbgn2b 393 27 progeny progeny JJ cord-329553-93tbgn2b 393 28 coronavirus coronavirus NN cord-329553-93tbgn2b 393 29 from from IN cord-329553-93tbgn2b 393 30 condensed condense VBN cord-329553-93tbgn2b 393 31 secretory secretory JJ cord-329553-93tbgn2b 393 32 proteins protein NNS cord-329553-93tbgn2b 393 33 at at IN cord-329553-93tbgn2b 393 34 the the DT cord-329553-93tbgn2b 393 35 exit exit NN cord-329553-93tbgn2b 393 36 from from IN cord-329553-93tbgn2b 393 37 the the DT cord-329553-93tbgn2b 393 38 trans trans NNP cord-329553-93tbgn2b 393 39 - - NNP cord-329553-93tbgn2b 393 40 Golgi Golgi NNP cord-329553-93tbgn2b 393 41 network network NN cord-329553-93tbgn2b 393 42 of of IN cord-329553-93tbgn2b 393 43 AtT20 AtT20 NNP cord-329553-93tbgn2b 393 44 cells cell NNS cord-329553-93tbgn2b 393 45 Identification identification NN cord-329553-93tbgn2b 393 46 by by IN cord-329553-93tbgn2b 393 47 anti anti JJ cord-329553-93tbgn2b 393 48 - - JJ cord-329553-93tbgn2b 393 49 idiotype idiotype JJ cord-329553-93tbgn2b 393 50 antibodies antibody NNS cord-329553-93tbgn2b 393 51 of of IN cord-329553-93tbgn2b 393 52 an an DT cord-329553-93tbgn2b 393 53 intracellular intracellular JJ cord-329553-93tbgn2b 393 54 membrane membrane NN cord-329553-93tbgn2b 393 55 protein protein NN cord-329553-93tbgn2b 393 56 that that WDT cord-329553-93tbgn2b 393 57 recognizes recognize VBZ cord-329553-93tbgn2b 393 58 a a DT cord-329553-93tbgn2b 393 59 mammalian mammalian JJ cord-329553-93tbgn2b 393 60 endoplasmic endoplasmic NN cord-329553-93tbgn2b 393 61 reticulum reticulum NN cord-329553-93tbgn2b 393 62 retention retention NN cord-329553-93tbgn2b 393 63 signal signal NN cord-329553-93tbgn2b 393 64 Primar Primar NNP cord-329553-93tbgn2b 393 65 y y NNP cord-329553-93tbgn2b 393 66 structure structure NN cord-329553-93tbgn2b 393 67 of of IN cord-329553-93tbgn2b 393 68 ß ß NN cord-329553-93tbgn2b 393 69 - - HYPH cord-329553-93tbgn2b 393 70 galactoside galactoside NN cord-329553-93tbgn2b 393 71 a2,6-sialyltransferase a2,6-sialyltransferase NN cord-329553-93tbgn2b 393 72 : : : cord-329553-93tbgn2b 393 73 conversion conversion NN cord-329553-93tbgn2b 393 74 of of IN cord-329553-93tbgn2b 393 75 membrane membrane NN cord-329553-93tbgn2b 393 76 - - HYPH cord-329553-93tbgn2b 393 77 bound bind VBN cord-329553-93tbgn2b 393 78 enzyme enzyme NN cord-329553-93tbgn2b 393 79 to to IN cord-329553-93tbgn2b 393 80 soluble soluble JJ cord-329553-93tbgn2b 393 81 forms form NNS cord-329553-93tbgn2b 393 82 by by IN cord-329553-93tbgn2b 393 83 cleavage cleavage NN cord-329553-93tbgn2b 393 84 of of IN cord-329553-93tbgn2b 393 85 the the DT cord-329553-93tbgn2b 393 86 NHrterminal NHrterminal NNP cord-329553-93tbgn2b 393 87 signal signal NN cord-329553-93tbgn2b 393 88 anchor anchor NN cord-329553-93tbgn2b 394 1 Transferrin Transferrin NNP cord-329553-93tbgn2b 394 2 receptors receptor NNS cord-329553-93tbgn2b 394 3 recycle recycle VBP cord-329553-93tbgn2b 394 4 to to IN cord-329553-93tbgn2b 394 5 cis cis NNP cord-329553-93tbgn2b 394 6 - - : cord-329553-93tbgn2b 394 7 and and CC cord-329553-93tbgn2b 394 8 middle middle JJ cord-329553-93tbgn2b 394 9 as as RB cord-329553-93tbgn2b 394 10 well well RB cord-329553-93tbgn2b 394 11 as as IN cord-329553-93tbgn2b 394 12 trans trans NNP cord-329553-93tbgn2b 394 13 - - NNP cord-329553-93tbgn2b 394 14 Golgi Golgi NNP cord-329553-93tbgn2b 394 15 cisternae cisternae NN cord-329553-93tbgn2b 394 16 in in IN cord-329553-93tbgn2b 394 17 Ig Ig NNP cord-329553-93tbgn2b 394 18 - - HYPH cord-329553-93tbgn2b 394 19 secreting secrete VBG cord-329553-93tbgn2b 394 20 myeloma myeloma NN cord-329553-93tbgn2b 394 21 cells cell NNS cord-329553-93tbgn2b 395 1 This this DT cord-329553-93tbgn2b 395 2 work work NN cord-329553-93tbgn2b 395 3 was be VBD cord-329553-93tbgn2b 395 4 supported support VBN cord-329553-93tbgn2b 395 5 by by IN cord-329553-93tbgn2b 395 6 a a DT cord-329553-93tbgn2b 395 7 research research NN cord-329553-93tbgn2b 395 8 fund fund NN cord-329553-93tbgn2b 395 9 from from IN cord-329553-93tbgn2b 395 10 the the DT cord-329553-93tbgn2b 395 11 Institute Institute NNP cord-329553-93tbgn2b 395 12 of of IN cord-329553-93tbgn2b 395 13 Molecular Molecular NNP cord-329553-93tbgn2b 395 14 and and CC cord-329553-93tbgn2b 395 15 Cell Cell NNP cord-329553-93tbgn2b 395 16 Biology Biology NNP cord-329553-93tbgn2b 395 17 , , , cord-329553-93tbgn2b 395 18 National National NNP cord-329553-93tbgn2b 395 19 University University NNP cord-329553-93tbgn2b 395 20 of of IN cord-329553-93tbgn2b 395 21 Singapore Singapore NNP cord-329553-93tbgn2b 395 22 , , , cord-329553-93tbgn2b 395 23 to to IN cord-329553-93tbgn2b 395 24 W W NNP cord-329553-93tbgn2b 395 25 . . . cord-329553-93tbgn2b 396 1 Hong Hong NNP cord-329553-93tbgn2b 396 2 .Received .Received NNP cord-329553-93tbgn2b 396 3 for for IN cord-329553-93tbgn2b 396 4 publication publication NN cord-329553-93tbgn2b 396 5 2 2 CD cord-329553-93tbgn2b 396 6 November November NNP cord-329553-93tbgn2b 396 7 1991 1991 CD cord-329553-93tbgn2b 396 8 and and CC cord-329553-93tbgn2b 396 9 in in IN cord-329553-93tbgn2b 396 10 revised revise VBN cord-329553-93tbgn2b 396 11 form form NN cord-329553-93tbgn2b 396 12 7 7 CD cord-329553-93tbgn2b 396 13 January January NNP cord-329553-93tbgn2b 396 14 1992 1992 CD cord-329553-93tbgn2b 396 15 . . .