id sid tid token lemma pos cord-328082-7c7slfbp 1 1 key key JJ cord-328082-7c7slfbp 1 2 : : : cord-328082-7c7slfbp 1 3 cord-328082 cord-328082 JJ cord-328082-7c7slfbp 1 4 - - HYPH cord-328082-7c7slfbp 1 5 7c7slfbp 7c7slfbp CD cord-328082-7c7slfbp 1 6 authors author NNS cord-328082-7c7slfbp 1 7 : : : cord-328082-7c7slfbp 2 1 nan nan NNP cord-328082-7c7slfbp 2 2 title title NN cord-328082-7c7slfbp 2 3 : : : cord-328082-7c7slfbp 2 4 Effect effect NN cord-328082-7c7slfbp 2 5 of of IN cord-328082-7c7slfbp 2 6 caffeine caffeine NN cord-328082-7c7slfbp 2 7 and and CC cord-328082-7c7slfbp 2 8 reduced reduce VBN cord-328082-7c7slfbp 2 9 temperature temperature NN cord-328082-7c7slfbp 2 10 ( ( -LRB- cord-328082-7c7slfbp 2 11 20 20 CD cord-328082-7c7slfbp 2 12 degrees degree NNS cord-328082-7c7slfbp 2 13 C c NN cord-328082-7c7slfbp 2 14 ) ) -RRB- cord-328082-7c7slfbp 2 15 on on IN cord-328082-7c7slfbp 2 16 the the DT cord-328082-7c7slfbp 2 17 organization organization NN cord-328082-7c7slfbp 2 18 of of IN cord-328082-7c7slfbp 2 19 the the DT cord-328082-7c7slfbp 2 20 pre pre JJ cord-328082-7c7slfbp 2 21 - - JJ cord-328082-7c7slfbp 2 22 Golgi Golgi NNP cord-328082-7c7slfbp 2 23 and and CC cord-328082-7c7slfbp 2 24 the the DT cord-328082-7c7slfbp 2 25 Golgi Golgi NNP cord-328082-7c7slfbp 2 26 stack stack NN cord-328082-7c7slfbp 2 27 membranes membrane NNS cord-328082-7c7slfbp 2 28 date date NN cord-328082-7c7slfbp 2 29 : : : cord-328082-7c7slfbp 2 30 1993 1993 CD cord-328082-7c7slfbp 2 31 - - SYM cord-328082-7c7slfbp 2 32 03 03 CD cord-328082-7c7slfbp 2 33 - - HYPH cord-328082-7c7slfbp 2 34 02 02 CD cord-328082-7c7slfbp 2 35 journal journal NN cord-328082-7c7slfbp 2 36 : : : cord-328082-7c7slfbp 2 37 J J NNP cord-328082-7c7slfbp 2 38 Cell Cell NNP cord-328082-7c7slfbp 2 39 Biol Biol NNP cord-328082-7c7slfbp 2 40 DOI DOI NNP cord-328082-7c7slfbp 2 41 : : : cord-328082-7c7slfbp 3 1 nan nan NNP cord-328082-7c7slfbp 3 2 sha sha NNP cord-328082-7c7slfbp 3 3 : : : cord-328082-7c7slfbp 3 4 a621b8667d221681dda8e3291d8b2c99a3636868 a621b8667d221681dda8e3291d8b2c99a3636868 NNP cord-328082-7c7slfbp 3 5 doc_id doc_id NNP cord-328082-7c7slfbp 3 6 : : : cord-328082-7c7slfbp 3 7 328082 328082 CD cord-328082-7c7slfbp 3 8 cord_uid cord_uid NNS cord-328082-7c7slfbp 3 9 : : : cord-328082-7c7slfbp 4 1 7c7slfbp 7c7slfbp LS cord-328082-7c7slfbp 5 1 In in IN cord-328082-7c7slfbp 5 2 the the DT cord-328082-7c7slfbp 5 3 present present JJ cord-328082-7c7slfbp 5 4 study study NN cord-328082-7c7slfbp 5 5 we -PRON- PRP cord-328082-7c7slfbp 5 6 have have VBP cord-328082-7c7slfbp 5 7 dissected dissect VBN cord-328082-7c7slfbp 5 8 the the DT cord-328082-7c7slfbp 5 9 transport transport NN cord-328082-7c7slfbp 5 10 pathways pathway NNS cord-328082-7c7slfbp 5 11 between between IN cord-328082-7c7slfbp 5 12 the the DT cord-328082-7c7slfbp 5 13 ER ER NNP cord-328082-7c7slfbp 5 14 and and CC cord-328082-7c7slfbp 5 15 the the DT cord-328082-7c7slfbp 5 16 Golgi Golgi NNP cord-328082-7c7slfbp 5 17 complex complex NN cord-328082-7c7slfbp 5 18 using use VBG cord-328082-7c7slfbp 5 19 a a DT cord-328082-7c7slfbp 5 20 recently recently RB cord-328082-7c7slfbp 5 21 introduced introduce VBN cord-328082-7c7slfbp 5 22 ( ( -LRB- cord-328082-7c7slfbp 5 23 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 5 24 , , , cord-328082-7c7slfbp 5 25 E. E. NNP cord-328082-7c7slfbp 5 26 , , , cord-328082-7c7slfbp 5 27 J. J. NNP cord-328082-7c7slfbp 5 28 Jantti Jantti NNP cord-328082-7c7slfbp 5 29 , , , cord-328082-7c7slfbp 5 30 V. V. NNP cord-328082-7c7slfbp 5 31 Makiranta Makiranta NNP cord-328082-7c7slfbp 5 32 , , , cord-328082-7c7slfbp 5 33 and and CC cord-328082-7c7slfbp 5 34 M. M. NNP cord-328082-7c7slfbp 5 35 Sariola Sariola NNP cord-328082-7c7slfbp 5 36 . . . cord-328082-7c7slfbp 6 1 1992 1992 CD cord-328082-7c7slfbp 6 2 . . . cord-328082-7c7slfbp 7 1 J. J. NNP cord-328082-7c7slfbp 7 2 Cell Cell NNP cord-328082-7c7slfbp 7 3 Sci Sci NNP cord-328082-7c7slfbp 7 4 . . . cord-328082-7c7slfbp 8 1 102:505- 102:505- CD cord-328082-7c7slfbp 8 2 513 513 CD cord-328082-7c7slfbp 8 3 ) ) -RRB- cord-328082-7c7slfbp 8 4 inhibition inhibition NN cord-328082-7c7slfbp 8 5 of of IN cord-328082-7c7slfbp 8 6 transport transport NN cord-328082-7c7slfbp 8 7 by by IN cord-328082-7c7slfbp 8 8 caffeine caffeine NN cord-328082-7c7slfbp 8 9 at at IN cord-328082-7c7slfbp 8 10 20 20 CD cord-328082-7c7slfbp 8 11 degrees degree NNS cord-328082-7c7slfbp 8 12 C. C. NNP cord-328082-7c7slfbp 8 13 Recovery Recovery NNP cord-328082-7c7slfbp 8 14 of of IN cord-328082-7c7slfbp 8 15 the the DT cord-328082-7c7slfbp 8 16 Golgi Golgi NNP cord-328082-7c7slfbp 8 17 complex complex NN cord-328082-7c7slfbp 8 18 from from IN cord-328082-7c7slfbp 8 19 brefeldin brefeldin NNP cord-328082-7c7slfbp 9 1 A a DT cord-328082-7c7slfbp 9 2 ( ( -LRB- cord-328082-7c7slfbp 9 3 BFA BFA NNP cord-328082-7c7slfbp 9 4 ) ) -RRB- cord-328082-7c7slfbp 9 5 treatment treatment NN cord-328082-7c7slfbp 9 6 was be VBD cord-328082-7c7slfbp 9 7 inhibited inhibit VBN cord-328082-7c7slfbp 9 8 by by IN cord-328082-7c7slfbp 9 9 caffeine caffeine NN cord-328082-7c7slfbp 9 10 at at IN cord-328082-7c7slfbp 9 11 reduced reduce VBN cord-328082-7c7slfbp 9 12 temperature temperature NN cord-328082-7c7slfbp 9 13 ( ( -LRB- cord-328082-7c7slfbp 9 14 20 20 CD cord-328082-7c7slfbp 9 15 degrees degree NNS cord-328082-7c7slfbp 9 16 C c NN cord-328082-7c7slfbp 9 17 ) ) -RRB- cord-328082-7c7slfbp 9 18 suggesting suggest VBG cord-328082-7c7slfbp 9 19 that that IN cord-328082-7c7slfbp 9 20 caffeine caffeine NN cord-328082-7c7slfbp 9 21 inhibits inhibit VBZ cord-328082-7c7slfbp 9 22 the the DT cord-328082-7c7slfbp 9 23 membrane membrane NN cord-328082-7c7slfbp 9 24 traffic traffic NN cord-328082-7c7slfbp 9 25 between between IN cord-328082-7c7slfbp 9 26 the the DT cord-328082-7c7slfbp 9 27 ER ER NNP cord-328082-7c7slfbp 9 28 and and CC cord-328082-7c7slfbp 9 29 the the DT cord-328082-7c7slfbp 9 30 Golgi Golgi NNP cord-328082-7c7slfbp 9 31 complex complex NN cord-328082-7c7slfbp 9 32 . . . cord-328082-7c7slfbp 10 1 Caffeine caffeine NN cord-328082-7c7slfbp 10 2 at at IN cord-328082-7c7slfbp 10 3 20 20 CD cord-328082-7c7slfbp 10 4 degrees degree NNS cord-328082-7c7slfbp 10 5 C c NN cord-328082-7c7slfbp 10 6 did do VBD cord-328082-7c7slfbp 10 7 not not RB cord-328082-7c7slfbp 10 8 inhibit inhibit VB cord-328082-7c7slfbp 10 9 the the DT cord-328082-7c7slfbp 10 10 BFA BFA NNP cord-328082-7c7slfbp 10 11 - - HYPH cord-328082-7c7slfbp 10 12 induced induce VBN cord-328082-7c7slfbp 10 13 retrograde retrograde JJ cord-328082-7c7slfbp 10 14 movement movement NN cord-328082-7c7slfbp 10 15 of of IN cord-328082-7c7slfbp 10 16 the the DT cord-328082-7c7slfbp 10 17 Golgi Golgi NNP cord-328082-7c7slfbp 10 18 membranes membrane NNS cord-328082-7c7slfbp 10 19 . . . cord-328082-7c7slfbp 11 1 Further further RB cord-328082-7c7slfbp 11 2 , , , cord-328082-7c7slfbp 11 3 incubation incubation NN cord-328082-7c7slfbp 11 4 of of IN cord-328082-7c7slfbp 11 5 the the DT cord-328082-7c7slfbp 11 6 cells cell NNS cord-328082-7c7slfbp 11 7 in in IN cord-328082-7c7slfbp 11 8 10 10 CD cord-328082-7c7slfbp 11 9 mM mm CD cord-328082-7c7slfbp 11 10 caffeine caffeine NN cord-328082-7c7slfbp 11 11 at at IN cord-328082-7c7slfbp 11 12 20 20 CD cord-328082-7c7slfbp 11 13 degrees degree NNS cord-328082-7c7slfbp 11 14 C c NN cord-328082-7c7slfbp 11 15 had have VBD cord-328082-7c7slfbp 11 16 profound profound JJ cord-328082-7c7slfbp 11 17 effects effect NNS cord-328082-7c7slfbp 11 18 on on IN cord-328082-7c7slfbp 11 19 the the DT cord-328082-7c7slfbp 11 20 distribution distribution NN cord-328082-7c7slfbp 11 21 and and CC cord-328082-7c7slfbp 11 22 the the DT cord-328082-7c7slfbp 11 23 organization organization NN cord-328082-7c7slfbp 11 24 of of IN cord-328082-7c7slfbp 11 25 the the DT cord-328082-7c7slfbp 11 26 pre pre JJ cord-328082-7c7slfbp 11 27 - - JJ cord-328082-7c7slfbp 11 28 Golgi Golgi NNP cord-328082-7c7slfbp 11 29 and and CC cord-328082-7c7slfbp 11 30 the the DT cord-328082-7c7slfbp 11 31 Golgi Golgi NNP cord-328082-7c7slfbp 11 32 stack stack NN cord-328082-7c7slfbp 11 33 membranes membrane NNS cord-328082-7c7slfbp 11 34 . . . cord-328082-7c7slfbp 12 1 Caffeine caffeine NN cord-328082-7c7slfbp 12 2 treatment treatment NN cord-328082-7c7slfbp 12 3 at at IN cord-328082-7c7slfbp 12 4 20 20 CD cord-328082-7c7slfbp 12 5 degrees degree NNS cord-328082-7c7slfbp 12 6 C c NN cord-328082-7c7slfbp 12 7 resulted result VBD cord-328082-7c7slfbp 12 8 in in IN cord-328082-7c7slfbp 12 9 a a DT cord-328082-7c7slfbp 12 10 selective selective JJ cord-328082-7c7slfbp 12 11 and and CC cord-328082-7c7slfbp 12 12 reversible reversible JJ cord-328082-7c7slfbp 12 13 translocation translocation NN cord-328082-7c7slfbp 12 14 of of IN cord-328082-7c7slfbp 12 15 the the DT cord-328082-7c7slfbp 12 16 pre- pre- JJ cord-328082-7c7slfbp 12 17 and and CC cord-328082-7c7slfbp 12 18 cis cis NNP cord-328082-7c7slfbp 12 19 - - HYPH cord-328082-7c7slfbp 12 20 Golgi Golgi NNP cord-328082-7c7slfbp 12 21 marker marker NN cord-328082-7c7slfbp 12 22 protein protein NN cord-328082-7c7slfbp 12 23 ( ( -LRB- cord-328082-7c7slfbp 12 24 p58 p58 NN cord-328082-7c7slfbp 12 25 ) ) -RRB- cord-328082-7c7slfbp 12 26 to to IN cord-328082-7c7slfbp 12 27 the the DT cord-328082-7c7slfbp 12 28 periphery periphery NN cord-328082-7c7slfbp 12 29 of of IN cord-328082-7c7slfbp 12 30 the the DT cord-328082-7c7slfbp 12 31 cell cell NN cord-328082-7c7slfbp 12 32 . . . cord-328082-7c7slfbp 13 1 This this DT cord-328082-7c7slfbp 13 2 caffeine caffeine NN cord-328082-7c7slfbp 13 3 - - HYPH cord-328082-7c7slfbp 13 4 induced induce VBN cord-328082-7c7slfbp 13 5 effect effect NN cord-328082-7c7slfbp 13 6 on on IN cord-328082-7c7slfbp 13 7 the the DT cord-328082-7c7slfbp 13 8 Golgi Golgi NNP cord-328082-7c7slfbp 13 9 complex complex NN cord-328082-7c7slfbp 13 10 was be VBD cord-328082-7c7slfbp 13 11 different different JJ cord-328082-7c7slfbp 13 12 from from IN cord-328082-7c7slfbp 13 13 that that DT cord-328082-7c7slfbp 13 14 induced induce VBN cord-328082-7c7slfbp 13 15 by by IN cord-328082-7c7slfbp 13 16 BFA BFA NNP cord-328082-7c7slfbp 13 17 , , , cord-328082-7c7slfbp 13 18 since since IN cord-328082-7c7slfbp 13 19 mannosidase mannosidase NNP cord-328082-7c7slfbp 13 20 II II NNP cord-328082-7c7slfbp 13 21 , , , cord-328082-7c7slfbp 13 22 a a DT cord-328082-7c7slfbp 13 23 Golgi Golgi NNP cord-328082-7c7slfbp 13 24 stack stack NN cord-328082-7c7slfbp 13 25 marker marker NN cord-328082-7c7slfbp 13 26 , , , cord-328082-7c7slfbp 13 27 remained remain VBD cord-328082-7c7slfbp 13 28 perinuclearly perinuclearly RB cord-328082-7c7slfbp 13 29 located locate VBN cord-328082-7c7slfbp 13 30 and and CC cord-328082-7c7slfbp 13 31 the the DT cord-328082-7c7slfbp 13 32 Golgi Golgi NNP cord-328082-7c7slfbp 13 33 stack stack NN cord-328082-7c7slfbp 13 34 coat coat NN cord-328082-7c7slfbp 13 35 protein protein NN cord-328082-7c7slfbp 13 36 , , , cord-328082-7c7slfbp 13 37 beta beta NN cord-328082-7c7slfbp 13 38 - - HYPH cord-328082-7c7slfbp 13 39 COP cop NN cord-328082-7c7slfbp 13 40 , , , cord-328082-7c7slfbp 13 41 was be VBD cord-328082-7c7slfbp 13 42 not not RB cord-328082-7c7slfbp 13 43 detached detach VBN cord-328082-7c7slfbp 13 44 from from IN cord-328082-7c7slfbp 13 45 Golgi Golgi NNP cord-328082-7c7slfbp 13 46 membranes membrane NNS cord-328082-7c7slfbp 13 47 in in IN cord-328082-7c7slfbp 13 48 the the DT cord-328082-7c7slfbp 13 49 presence presence NN cord-328082-7c7slfbp 13 50 of of IN cord-328082-7c7slfbp 13 51 10 10 CD cord-328082-7c7slfbp 13 52 mM mm CD cord-328082-7c7slfbp 13 53 caffeine caffeine NN cord-328082-7c7slfbp 13 54 at at IN cord-328082-7c7slfbp 13 55 20 20 CD cord-328082-7c7slfbp 13 56 degrees degree NNS cord-328082-7c7slfbp 14 1 C. C. NNP cord-328082-7c7slfbp 14 2 Electron Electron NNP cord-328082-7c7slfbp 14 3 microscopic microscopic JJ cord-328082-7c7slfbp 14 4 analysis analysis NN cord-328082-7c7slfbp 14 5 showed show VBD cord-328082-7c7slfbp 14 6 that that IN cord-328082-7c7slfbp 14 7 , , , cord-328082-7c7slfbp 14 8 in in IN cord-328082-7c7slfbp 14 9 the the DT cord-328082-7c7slfbp 14 10 presence presence NN cord-328082-7c7slfbp 14 11 of of IN cord-328082-7c7slfbp 14 12 caffeine caffeine NN cord-328082-7c7slfbp 14 13 at at IN cord-328082-7c7slfbp 14 14 20 20 CD cord-328082-7c7slfbp 14 15 degrees degree NNS cord-328082-7c7slfbp 14 16 C c NN cord-328082-7c7slfbp 14 17 , , , cord-328082-7c7slfbp 14 18 the the DT cord-328082-7c7slfbp 14 19 morphology morphology NN cord-328082-7c7slfbp 14 20 of of IN cord-328082-7c7slfbp 14 21 the the DT cord-328082-7c7slfbp 14 22 Golgi Golgi NNP cord-328082-7c7slfbp 14 23 stack stack NN cord-328082-7c7slfbp 14 24 was be VBD cord-328082-7c7slfbp 14 25 altered alter VBN cord-328082-7c7slfbp 14 26 and and CC cord-328082-7c7slfbp 14 27 accumulation accumulation NN cord-328082-7c7slfbp 14 28 of of IN cord-328082-7c7slfbp 14 29 numerous numerous JJ cord-328082-7c7slfbp 14 30 small small JJ cord-328082-7c7slfbp 14 31 vesicles vesicle NNS cord-328082-7c7slfbp 14 32 in in IN cord-328082-7c7slfbp 14 33 the the DT cord-328082-7c7slfbp 14 34 Golgi Golgi NNP cord-328082-7c7slfbp 14 35 region region NN cord-328082-7c7slfbp 14 36 was be VBD cord-328082-7c7slfbp 14 37 observed observe VBN cord-328082-7c7slfbp 14 38 . . . cord-328082-7c7slfbp 15 1 The the DT cord-328082-7c7slfbp 15 2 results result NNS cord-328082-7c7slfbp 15 3 in in IN cord-328082-7c7slfbp 15 4 the the DT cord-328082-7c7slfbp 15 5 present present JJ cord-328082-7c7slfbp 15 6 study study NN cord-328082-7c7slfbp 15 7 suggest suggest VBP cord-328082-7c7slfbp 15 8 that that IN cord-328082-7c7slfbp 15 9 caffeine caffeine NN cord-328082-7c7slfbp 15 10 at at IN cord-328082-7c7slfbp 15 11 reduced reduce VBN cord-328082-7c7slfbp 15 12 temperature temperature NN cord-328082-7c7slfbp 15 13 ( ( -LRB- cord-328082-7c7slfbp 15 14 20 20 CD cord-328082-7c7slfbp 15 15 degrees degree NNS cord-328082-7c7slfbp 15 16 C c NN cord-328082-7c7slfbp 15 17 ) ) -RRB- cord-328082-7c7slfbp 15 18 reveals reveal VBZ cord-328082-7c7slfbp 15 19 a a DT cord-328082-7c7slfbp 15 20 functional functional JJ cord-328082-7c7slfbp 15 21 interface interface NN cord-328082-7c7slfbp 15 22 between between IN cord-328082-7c7slfbp 15 23 the the DT cord-328082-7c7slfbp 15 24 pre pre JJ cord-328082-7c7slfbp 15 25 - - JJ cord-328082-7c7slfbp 15 26 Golgi Golgi NNP cord-328082-7c7slfbp 15 27 and and CC cord-328082-7c7slfbp 15 28 the the DT cord-328082-7c7slfbp 15 29 Golgi Golgi NNP cord-328082-7c7slfbp 15 30 stack stack NN cord-328082-7c7slfbp 15 31 . . . cord-328082-7c7slfbp 16 1 T T NNP cord-328082-7c7slfbp 16 2 RANSPORT RANSPORT NNP cord-328082-7c7slfbp 16 3 inhibitors inhibitor NNS cord-328082-7c7slfbp 16 4 have have VBP cord-328082-7c7slfbp 16 5 been be VBN cord-328082-7c7slfbp 16 6 important important JJ cord-328082-7c7slfbp 16 7 tools tool NNS cord-328082-7c7slfbp 16 8 in in IN cord-328082-7c7slfbp 16 9 revealing reveal VBG cord-328082-7c7slfbp 16 10 the the DT cord-328082-7c7slfbp 16 11 existence existence NN cord-328082-7c7slfbp 16 12 of of IN cord-328082-7c7slfbp 16 13 functionally functionally RB cord-328082-7c7slfbp 16 14 distinct distinct JJ cord-328082-7c7slfbp 16 15 compartments compartment NNS cord-328082-7c7slfbp 16 16 within within IN cord-328082-7c7slfbp 16 17 the the DT cord-328082-7c7slfbp 16 18 cell cell NN cord-328082-7c7slfbp 16 19 . . . cord-328082-7c7slfbp 17 1 Site site NN cord-328082-7c7slfbp 17 2 - - HYPH cord-328082-7c7slfbp 17 3 specific specific JJ cord-328082-7c7slfbp 17 4 perturbation perturbation NN cord-328082-7c7slfbp 17 5 of of IN cord-328082-7c7slfbp 17 6 the the DT cord-328082-7c7slfbp 17 7 endomembrane endomembrane NNP cord-328082-7c7slfbp 17 8 architecture architecture NN cord-328082-7c7slfbp 17 9 often often RB cord-328082-7c7slfbp 17 10 results result VBZ cord-328082-7c7slfbp 17 11 in in IN cord-328082-7c7slfbp 17 12 the the DT cord-328082-7c7slfbp 17 13 accumulation accumulation NN cord-328082-7c7slfbp 17 14 of of IN cord-328082-7c7slfbp 17 15 transported transport VBN cord-328082-7c7slfbp 17 16 proteins protein NNS cord-328082-7c7slfbp 17 17 and and CC cord-328082-7c7slfbp 17 18 membranes membrane NNS cord-328082-7c7slfbp 17 19 at at IN cord-328082-7c7slfbp 17 20 specific specific JJ cord-328082-7c7slfbp 17 21 steps step NNS cord-328082-7c7slfbp 17 22 along along IN cord-328082-7c7slfbp 17 23 the the DT cord-328082-7c7slfbp 17 24 transport transport NN cord-328082-7c7slfbp 17 25 pathways pathway NNS cord-328082-7c7slfbp 17 26 . . . cord-328082-7c7slfbp 18 1 In in IN cord-328082-7c7slfbp 18 2 many many JJ cord-328082-7c7slfbp 18 3 cases case NNS cord-328082-7c7slfbp 18 4 the the DT cord-328082-7c7slfbp 18 5 use use NN cord-328082-7c7slfbp 18 6 of of IN cord-328082-7c7slfbp 18 7 inhibitors inhibitor NNS cord-328082-7c7slfbp 18 8 has have VBZ cord-328082-7c7slfbp 18 9 made make VBN cord-328082-7c7slfbp 18 10 it -PRON- PRP cord-328082-7c7slfbp 18 11 possible possible JJ cord-328082-7c7slfbp 18 12 to to TO cord-328082-7c7slfbp 18 13 identify identify VB cord-328082-7c7slfbp 18 14 functions function NNS cord-328082-7c7slfbp 18 15 and and CC cord-328082-7c7slfbp 18 16 operating operate VBG cord-328082-7c7slfbp 18 17 components component NNS cord-328082-7c7slfbp 18 18 resident resident NN cord-328082-7c7slfbp 18 19 in in IN cord-328082-7c7slfbp 18 20 the the DT cord-328082-7c7slfbp 18 21 sites site NNS cord-328082-7c7slfbp 18 22 of of IN cord-328082-7c7slfbp 18 23 question question NN cord-328082-7c7slfbp 18 24 . . . cord-328082-7c7slfbp 19 1 Reduced reduce VBN cord-328082-7c7slfbp 19 2 temperature temperature NN cord-328082-7c7slfbp 19 3 has have VBZ cord-328082-7c7slfbp 19 4 been be VBN cord-328082-7c7slfbp 19 5 widely widely RB cord-328082-7c7slfbp 19 6 used use VBN cord-328082-7c7slfbp 19 7 to to TO cord-328082-7c7slfbp 19 8 dissect dissect VB cord-328082-7c7slfbp 19 9 transport transport NN cord-328082-7c7slfbp 19 10 steps step NNS cord-328082-7c7slfbp 19 11 in in IN cord-328082-7c7slfbp 19 12 the the DT cord-328082-7c7slfbp 19 13 exocytotic exocytotic JJ cord-328082-7c7slfbp 19 14 transport transport NN cord-328082-7c7slfbp 19 15 pathway pathway NN cord-328082-7c7slfbp 19 16 ( ( -LRB- cord-328082-7c7slfbp 19 17 for for IN cord-328082-7c7slfbp 19 18 review review NN cord-328082-7c7slfbp 19 19 see see VB cord-328082-7c7slfbp 19 20 Saraste Saraste NNP cord-328082-7c7slfbp 19 21 and and CC cord-328082-7c7slfbp 19 22 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 19 23 , , , cord-328082-7c7slfbp 19 24 1992 1992 CD cord-328082-7c7slfbp 19 25 ) ) -RRB- cord-328082-7c7slfbp 19 26 . . . cord-328082-7c7slfbp 20 1 Specifically specifically RB cord-328082-7c7slfbp 20 2 , , , cord-328082-7c7slfbp 20 3 incubation incubation NN cord-328082-7c7slfbp 20 4 at at IN cord-328082-7c7slfbp 20 5 15~ 15~ CD cord-328082-7c7slfbp 20 6 blocks block VBZ cord-328082-7c7slfbp 20 7 the the DT cord-328082-7c7slfbp 20 8 transport transport NN cord-328082-7c7slfbp 20 9 at at IN cord-328082-7c7slfbp 20 10 the the DT cord-328082-7c7slfbp 20 11 pre pre JJ cord-328082-7c7slfbp 20 12 - - JJ cord-328082-7c7slfbp 20 13 Golgi golgi JJ cord-328082-7c7slfbp 20 14 level level NN cord-328082-7c7slfbp 20 15 ( ( -LRB- cord-328082-7c7slfbp 20 16 Saraste Saraste NNP cord-328082-7c7slfbp 20 17 and and CC cord-328082-7c7slfbp 20 18 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 20 19 , , , cord-328082-7c7slfbp 20 20 1984 1984 CD cord-328082-7c7slfbp 20 21 ) ) -RRB- cord-328082-7c7slfbp 20 22 and and CC cord-328082-7c7slfbp 20 23 has have VBZ cord-328082-7c7slfbp 20 24 led lead VBN cord-328082-7c7slfbp 20 25 to to IN cord-328082-7c7slfbp 20 26 the the DT cord-328082-7c7slfbp 20 27 characterization characterization NN cord-328082-7c7slfbp 20 28 of of IN cord-328082-7c7slfbp 20 29 p58 p58 NN cord-328082-7c7slfbp 20 30 , , , cord-328082-7c7slfbp 20 31 a a DT cord-328082-7c7slfbp 20 32 resident resident JJ cord-328082-7c7slfbp 20 33 protein protein NN cord-328082-7c7slfbp 20 34 in in IN cord-328082-7c7slfbp 20 35 the the DT cord-328082-7c7slfbp 20 36 pre pre JJ cord-328082-7c7slfbp 20 37 - - JJ cord-328082-7c7slfbp 20 38 and and CC cord-328082-7c7slfbp 20 39 cis cis NNP cord-328082-7c7slfbp 20 40 - - HYPH cord-328082-7c7slfbp 20 41 Golgi Golgi NNP cord-328082-7c7slfbp 20 42 compartments compartment NNS cord-328082-7c7slfbp 20 43 ( ( -LRB- cord-328082-7c7slfbp 20 44 Saraste Saraste NNP cord-328082-7c7slfbp 20 45 et et NNP cord-328082-7c7slfbp 20 46 al al NNP cord-328082-7c7slfbp 20 47 . . NNP cord-328082-7c7slfbp 20 48 , , , cord-328082-7c7slfbp 20 49 1987 1987 CD cord-328082-7c7slfbp 20 50 ) ) -RRB- cord-328082-7c7slfbp 20 51 . . . cord-328082-7c7slfbp 21 1 The the DT cord-328082-7c7slfbp 21 2 depletion depletion NN cord-328082-7c7slfbp 21 3 of of IN cord-328082-7c7slfbp 21 4 cellular cellular JJ cord-328082-7c7slfbp 21 5 ATP ATP NNP cord-328082-7c7slfbp 21 6 has have VBZ cord-328082-7c7slfbp 21 7 been be VBN cord-328082-7c7slfbp 21 8 shown show VBN cord-328082-7c7slfbp 21 9 to to TO cord-328082-7c7slfbp 21 10 inhibit inhibit VB cord-328082-7c7slfbp 21 11 distinct distinct JJ cord-328082-7c7slfbp 21 12 steps step NNS cord-328082-7c7slfbp 21 13 in in IN cord-328082-7c7slfbp 21 14 vesicular vesicular JJ cord-328082-7c7slfbp 21 15 transport transport NN cord-328082-7c7slfbp 21 16 ( ( -LRB- cord-328082-7c7slfbp 21 17 Balch Balch NNP cord-328082-7c7slfbp 21 18 et et NNP cord-328082-7c7slfbp 21 19 al al NNP cord-328082-7c7slfbp 21 20 . . NNP cord-328082-7c7slfbp 21 21 , , , cord-328082-7c7slfbp 21 22 1986 1986 CD cord-328082-7c7slfbp 21 23 ; ; : cord-328082-7c7slfbp 21 24 Tartakoff Tartakoff NNP cord-328082-7c7slfbp 21 25 , , , cord-328082-7c7slfbp 21 26 1986 1986 CD cord-328082-7c7slfbp 21 27 ) ) -RRB- cord-328082-7c7slfbp 21 28 . . . cord-328082-7c7slfbp 22 1 The the DT cord-328082-7c7slfbp 22 2 important important JJ cord-328082-7c7slfbp 22 3 role role NN cord-328082-7c7slfbp 22 4 of of IN cord-328082-7c7slfbp 22 5 calcium calcium NN cord-328082-7c7slfbp 22 6 in in IN cord-328082-7c7slfbp 22 7 vesicular vesicular JJ cord-328082-7c7slfbp 22 8 traffic traffic NN cord-328082-7c7slfbp 22 9 between between IN cord-328082-7c7slfbp 22 10 the the DT cord-328082-7c7slfbp 22 11 ER ER NNP cord-328082-7c7slfbp 22 12 and and CC cord-328082-7c7slfbp 22 13 the the DT cord-328082-7c7slfbp 22 14 Golgi Golgi NNP cord-328082-7c7slfbp 22 15 compartment compartment NN cord-328082-7c7slfbp 22 16 has have VBZ cord-328082-7c7slfbp 22 17 become become VBN cord-328082-7c7slfbp 22 18 evident evident JJ cord-328082-7c7slfbp 22 19 from from IN cord-328082-7c7slfbp 22 20 in in IN cord-328082-7c7slfbp 22 21 vitro vitro FW cord-328082-7c7slfbp 22 22 studies study NNS cord-328082-7c7slfbp 22 23 ( ( -LRB- cord-328082-7c7slfbp 22 24 Beckers Beckers NNP cord-328082-7c7slfbp 22 25 and and CC cord-328082-7c7slfbp 22 26 Balch Balch NNP cord-328082-7c7slfbp 22 27 , , , cord-328082-7c7slfbp 22 28 1989 1989 CD cord-328082-7c7slfbp 22 29 ; ; : cord-328082-7c7slfbp 22 30 Sambrook Sambrook NNP cord-328082-7c7slfbp 22 31 , , , cord-328082-7c7slfbp 22 32 1990 1990 CD cord-328082-7c7slfbp 22 33 ) ) -RRB- cord-328082-7c7slfbp 22 34 . . . cord-328082-7c7slfbp 23 1 Also also RB cord-328082-7c7slfbp 23 2 , , , cord-328082-7c7slfbp 23 3 the the DT cord-328082-7c7slfbp 23 4 perturbation perturbation NN cord-328082-7c7slfbp 23 5 of of IN cord-328082-7c7slfbp 23 6 intraceUular intraceUular NNP cord-328082-7c7slfbp 23 7 Ca2 ca2 CD cord-328082-7c7slfbp 23 8 + + CC cord-328082-7c7slfbp 24 1 -levels -level NNS cord-328082-7c7slfbp 24 2 blocks block VBZ cord-328082-7c7slfbp 24 3 the the DT cord-328082-7c7slfbp 24 4 movement movement NN cord-328082-7c7slfbp 24 5 of of IN cord-328082-7c7slfbp 24 6 secretory secretory JJ cord-328082-7c7slfbp 24 7 proteins protein NNS cord-328082-7c7slfbp 24 8 out out RP cord-328082-7c7slfbp 24 9 from from IN cord-328082-7c7slfbp 24 10 the the DT cord-328082-7c7slfbp 24 11 rough rough JJ cord-328082-7c7slfbp 24 12 ER er NN cord-328082-7c7slfbp 24 13 ( ( -LRB- cord-328082-7c7slfbp 24 14 Ix)dish ix)dish NN cord-328082-7c7slfbp 24 15 and and CC cord-328082-7c7slfbp 24 16 Kong Kong NNP cord-328082-7c7slfbp 24 17 , , , cord-328082-7c7slfbp 24 18 1990 1990 CD cord-328082-7c7slfbp 24 19 ) ) -RRB- cord-328082-7c7slfbp 24 20 . . . cord-328082-7c7slfbp 25 1 In in IN cord-328082-7c7slfbp 25 2 addition addition NN cord-328082-7c7slfbp 25 3 , , , cord-328082-7c7slfbp 25 4 lowered lower VBD cord-328082-7c7slfbp 25 5 extraceUular extraceuular JJ cord-328082-7c7slfbp 26 1 pH pH NNP cord-328082-7c7slfbp 26 2 has have VBZ cord-328082-7c7slfbp 26 3 been be VBN cord-328082-7c7slfbp 26 4 reported report VBN cord-328082-7c7slfbp 26 5 to to TO cord-328082-7c7slfbp 26 6 arrest arrest VB cord-328082-7c7slfbp 26 7 the the DT cord-328082-7c7slfbp 26 8 transport transport NN cord-328082-7c7slfbp 26 9 of of IN cord-328082-7c7slfbp 26 10 influenza influenza NN cord-328082-7c7slfbp 26 11 virus virus NN cord-328082-7c7slfbp 26 12 hemagglutinin hemagglutinin NN cord-328082-7c7slfbp 26 13 at at IN cord-328082-7c7slfbp 26 14 the the DT cord-328082-7c7slfbp 26 15 pre pre JJ cord-328082-7c7slfbp 26 16 - - JJ cord-328082-7c7slfbp 26 17 Golgi golgi JJ cord-328082-7c7slfbp 26 18 level level NN cord-328082-7c7slfbp 27 1 ( ( -LRB- cord-328082-7c7slfbp 27 2 Matlin Matlin NNP cord-328082-7c7slfbp 27 3 et et NNP cord-328082-7c7slfbp 27 4 al al NNP cord-328082-7c7slfbp 27 5 . . NNP cord-328082-7c7slfbp 27 6 , , , cord-328082-7c7slfbp 27 7 1988 1988 CD cord-328082-7c7slfbp 27 8 ) ) -RRB- cord-328082-7c7slfbp 27 9 . . . cord-328082-7c7slfbp 28 1 The the DT cord-328082-7c7slfbp 28 2 central central JJ cord-328082-7c7slfbp 28 3 role role NN cord-328082-7c7slfbp 28 4 of of IN cord-328082-7c7slfbp 28 5 GTP GTP NNP cord-328082-7c7slfbp 28 6 in in IN cord-328082-7c7slfbp 28 7 the the DT cord-328082-7c7slfbp 28 8 vesicular vesicular JJ cord-328082-7c7slfbp 28 9 transport transport NN cord-328082-7c7slfbp 28 10 has have VBZ cord-328082-7c7slfbp 28 11 become become VBN cord-328082-7c7slfbp 28 12 evident evident JJ cord-328082-7c7slfbp 28 13 from from IN cord-328082-7c7slfbp 28 14 in in IN cord-328082-7c7slfbp 28 15 vitro vitro FW cord-328082-7c7slfbp 28 16 studies study NNS cord-328082-7c7slfbp 28 17 where where WRB cord-328082-7c7slfbp 28 18 the the DT cord-328082-7c7slfbp 28 19 nonhydrolyz nonhydrolyz JJ cord-328082-7c7slfbp 28 20 - - HYPH cord-328082-7c7slfbp 28 21 able able JJ cord-328082-7c7slfbp 28 22 GTP gtp NN cord-328082-7c7slfbp 28 23 - - HYPH cord-328082-7c7slfbp 28 24 analogue analogue NN cord-328082-7c7slfbp 28 25 , , , cord-328082-7c7slfbp 28 26 GTP-3,-S GTP-3,-S NNP cord-328082-7c7slfbp 28 27 , , , cord-328082-7c7slfbp 28 28 has have VBZ cord-328082-7c7slfbp 28 29 been be VBN cord-328082-7c7slfbp 28 30 used use VBN cord-328082-7c7slfbp 28 31 to to TO cord-328082-7c7slfbp 28 32 inhibit inhibit VB cord-328082-7c7slfbp 28 33 ERto ERto NNP cord-328082-7c7slfbp 28 34 - - HYPH cord-328082-7c7slfbp 28 35 Golgi Golgi NNP cord-328082-7c7slfbp 28 36 transport transport NN cord-328082-7c7slfbp 28 37 both both DT cord-328082-7c7slfbp 28 38 in in IN cord-328082-7c7slfbp 28 39 mammalian mammalian JJ cord-328082-7c7slfbp 28 40 and and CC cord-328082-7c7slfbp 28 41 yeast yeast NN cord-328082-7c7slfbp 28 42 ceils ceil NNS cord-328082-7c7slfbp 28 43 ( ( -LRB- cord-328082-7c7slfbp 28 44 Beckers Beckers NNP cord-328082-7c7slfbp 28 45 and and CC cord-328082-7c7slfbp 28 46 Balch Balch NNP cord-328082-7c7slfbp 28 47 , , , cord-328082-7c7slfbp 28 48 1989 1989 CD cord-328082-7c7slfbp 28 49 ; ; : cord-328082-7c7slfbp 28 50 Ruohola Ruohola NNP cord-328082-7c7slfbp 28 51 et et NNP cord-328082-7c7slfbp 28 52 al al NNP cord-328082-7c7slfbp 28 53 . . NNP cord-328082-7c7slfbp 28 54 , , , cord-328082-7c7slfbp 28 55 1988 1988 CD cord-328082-7c7slfbp 28 56 ) ) -RRB- cord-328082-7c7slfbp 28 57 . . . cord-328082-7c7slfbp 29 1 Recent recent JJ cord-328082-7c7slfbp 29 2 localization localization NN cord-328082-7c7slfbp 29 3 of of IN cord-328082-7c7slfbp 29 4 the the DT cord-328082-7c7slfbp 29 5 small small JJ cord-328082-7c7slfbp 29 6 GTP gtp NN cord-328082-7c7slfbp 29 7 - - HYPH cord-328082-7c7slfbp 29 8 binding bind VBG cord-328082-7c7slfbp 29 9 proteins protein NNS cord-328082-7c7slfbp 29 10 rablb rablb VBN cord-328082-7c7slfbp 29 11 and and CC cord-328082-7c7slfbp 29 12 rab2 rab2 JJ cord-328082-7c7slfbp 29 13 to to IN cord-328082-7c7slfbp 29 14 the the DT cord-328082-7c7slfbp 29 15 membranes membrane NNS cord-328082-7c7slfbp 29 16 responsible responsible JJ cord-328082-7c7slfbp 29 17 for for IN cord-328082-7c7slfbp 29 18 the the DT cord-328082-7c7slfbp 29 19 ER ER NNP cord-328082-7c7slfbp 29 20 - - HYPH cord-328082-7c7slfbp 29 21 to to IN cord-328082-7c7slfbp 29 22 - - HYPH cord-328082-7c7slfbp 29 23 Golgi golgi NN cord-328082-7c7slfbp 29 24 transport transport NN cord-328082-7c7slfbp 29 25 has have VBZ cord-328082-7c7slfbp 29 26 further further RB cord-328082-7c7slfbp 29 27 emphasized emphasize VBN cord-328082-7c7slfbp 29 28 the the DT cord-328082-7c7slfbp 29 29 role role NN cord-328082-7c7slfbp 29 30 of of IN cord-328082-7c7slfbp 29 31 GTP GTP NNP cord-328082-7c7slfbp 29 32 as as IN cord-328082-7c7slfbp 29 33 a a DT cord-328082-7c7slfbp 29 34 regulator regulator NN cord-328082-7c7slfbp 29 35 of of IN cord-328082-7c7slfbp 29 36 ER er NN cord-328082-7c7slfbp 29 37 - - HYPH cord-328082-7c7slfbp 29 38 to to IN cord-328082-7c7slfbp 29 39 - - HYPH cord-328082-7c7slfbp 29 40 Golgi golgi NN cord-328082-7c7slfbp 29 41 membrane membrane NN cord-328082-7c7slfbp 29 42 traffic traffic NN cord-328082-7c7slfbp 29 43 ( ( -LRB- cord-328082-7c7slfbp 29 44 Chavrier Chavrier NNP cord-328082-7c7slfbp 29 45 et et NNP cord-328082-7c7slfbp 29 46 al al NNP cord-328082-7c7slfbp 29 47 . . NNP cord-328082-7c7slfbp 29 48 , , , cord-328082-7c7slfbp 29 49 1990 1990 CD cord-328082-7c7slfbp 29 50 ; ; : cord-328082-7c7slfbp 29 51 Plutner Plutner NNP cord-328082-7c7slfbp 29 52 et et NNP cord-328082-7c7slfbp 29 53 al al NNP cord-328082-7c7slfbp 29 54 . . NNP cord-328082-7c7slfbp 29 55 , , , cord-328082-7c7slfbp 29 56 1991 1991 CD cord-328082-7c7slfbp 29 57 ) ) -RRB- cord-328082-7c7slfbp 29 58 . . . cord-328082-7c7slfbp 30 1 Interestingly interestingly RB cord-328082-7c7slfbp 30 2 , , , cord-328082-7c7slfbp 30 3 there there EX cord-328082-7c7slfbp 30 4 is be VBZ cord-328082-7c7slfbp 30 5 also also RB cord-328082-7c7slfbp 30 6 evidence evidence NN cord-328082-7c7slfbp 30 7 for for IN cord-328082-7c7slfbp 30 8 the the DT cord-328082-7c7slfbp 30 9 participation participation NN cord-328082-7c7slfbp 30 10 of of IN cord-328082-7c7slfbp 30 11 trimeric trimeric NNP cord-328082-7c7slfbp 30 12 GTP GTP NNP cord-328082-7c7slfbp 30 13 - - HYPH cord-328082-7c7slfbp 30 14 binding bind VBG cord-328082-7c7slfbp 30 15 proteins protein NNS cord-328082-7c7slfbp 30 16 in in IN cord-328082-7c7slfbp 30 17 the the DT cord-328082-7c7slfbp 30 18 regulation regulation NN cord-328082-7c7slfbp 30 19 of of IN cord-328082-7c7slfbp 30 20 membrane membrane NN cord-328082-7c7slfbp 30 21 traffic traffic NN cord-328082-7c7slfbp 30 22 ( ( -LRB- cord-328082-7c7slfbp 30 23 Stow Stow NNP cord-328082-7c7slfbp 30 24 et et FW cord-328082-7c7slfbp 30 25 al al NNP cord-328082-7c7slfbp 30 26 . . NNP cord-328082-7c7slfbp 30 27 , , , cord-328082-7c7slfbp 30 28 1991 1991 CD cord-328082-7c7slfbp 30 29 ; ; : cord-328082-7c7slfbp 31 1 Barr Barr NNP cord-328082-7c7slfbp 31 2 et et NNP cord-328082-7c7slfbp 31 3 al al NNP cord-328082-7c7slfbp 31 4 . . NNP cord-328082-7c7slfbp 31 5 , , , cord-328082-7c7slfbp 31 6 1991 1991 CD cord-328082-7c7slfbp 31 7 ) ) -RRB- cord-328082-7c7slfbp 31 8 , , , cord-328082-7c7slfbp 31 9 thus thus RB cord-328082-7c7slfbp 31 10 revealing reveal VBG cord-328082-7c7slfbp 31 11 a a DT cord-328082-7c7slfbp 31 12 link link NN cord-328082-7c7slfbp 31 13 between between IN cord-328082-7c7slfbp 31 14 signal signal NN cord-328082-7c7slfbp 31 15 transduction transduction NN cord-328082-7c7slfbp 31 16 and and CC cord-328082-7c7slfbp 31 17 the the DT cord-328082-7c7slfbp 31 18 processes process NNS cord-328082-7c7slfbp 31 19 in in IN cord-328082-7c7slfbp 31 20 the the DT cord-328082-7c7slfbp 31 21 intracellular intracellular JJ cord-328082-7c7slfbp 31 22 membrane membrane NN cord-328082-7c7slfbp 31 23 traffic traffic NN cord-328082-7c7slfbp 31 24 ( ( -LRB- cord-328082-7c7slfbp 31 25 for for IN cord-328082-7c7slfbp 31 26 review review NN cord-328082-7c7slfbp 31 27 see see VBP cord-328082-7c7slfbp 31 28 Balch Balch NNP cord-328082-7c7slfbp 31 29 , , , cord-328082-7c7slfbp 31 30 1992 1992 CD cord-328082-7c7slfbp 31 31 ; ; : cord-328082-7c7slfbp 31 32 Barr Barr NNP cord-328082-7c7slfbp 31 33 et et NNP cord-328082-7c7slfbp 31 34 al al NNP cord-328082-7c7slfbp 31 35 . . NNP cord-328082-7c7slfbp 31 36 , , , cord-328082-7c7slfbp 31 37 1992 1992 CD cord-328082-7c7slfbp 31 38 ) ) -RRB- cord-328082-7c7slfbp 31 39 . . . cord-328082-7c7slfbp 32 1 Okadaic okadaic JJ cord-328082-7c7slfbp 32 2 acid acid NN cord-328082-7c7slfbp 32 3 , , , cord-328082-7c7slfbp 32 4 a a DT cord-328082-7c7slfbp 32 5 phosphatase phosphatase NN cord-328082-7c7slfbp 32 6 inhibitor inhibitor NN cord-328082-7c7slfbp 32 7 , , , cord-328082-7c7slfbp 32 8 has have VBZ cord-328082-7c7slfbp 32 9 been be VBN cord-328082-7c7slfbp 32 10 shown show VBN cord-328082-7c7slfbp 32 11 to to TO cord-328082-7c7slfbp 32 12 inhibit inhibit VB cord-328082-7c7slfbp 32 13 ER er NN cord-328082-7c7slfbp 32 14 - - HYPH cord-328082-7c7slfbp 32 15 to to IN cord-328082-7c7slfbp 32 16 - - HYPH cord-328082-7c7slfbp 32 17 Golgi golgi NN cord-328082-7c7slfbp 32 18 transport transport NN cord-328082-7c7slfbp 32 19 ( ( -LRB- cord-328082-7c7slfbp 32 20 Davison Davison NNP cord-328082-7c7slfbp 32 21 et et FW cord-328082-7c7slfbp 32 22 al al NNP cord-328082-7c7slfbp 32 23 . . NNP cord-328082-7c7slfbp 32 24 , , , cord-328082-7c7slfbp 32 25 1992 1992 CD cord-328082-7c7slfbp 32 26 ) ) -RRB- cord-328082-7c7slfbp 32 27 and and CC cord-328082-7c7slfbp 32 28 result result VB cord-328082-7c7slfbp 32 29 in in IN cord-328082-7c7slfbp 32 30 the the DT cord-328082-7c7slfbp 32 31 fragmentation fragmentation NN cord-328082-7c7slfbp 32 32 and and CC cord-328082-7c7slfbp 32 33 dispersal dispersal NN cord-328082-7c7slfbp 32 34 of of IN cord-328082-7c7slfbp 32 35 the the DT cord-328082-7c7slfbp 32 36 Golgi Golgi NNP cord-328082-7c7slfbp 32 37 apparatus apparatus NN cord-328082-7c7slfbp 32 38 ( ( -LRB- cord-328082-7c7slfbp 32 39 Lucocq Lucocq NNP cord-328082-7c7slfbp 32 40 et et FW cord-328082-7c7slfbp 32 41 al al NNP cord-328082-7c7slfbp 32 42 . . NNP cord-328082-7c7slfbp 32 43 , , , cord-328082-7c7slfbp 32 44 1991 1991 CD cord-328082-7c7slfbp 32 45 ) ) -RRB- cord-328082-7c7slfbp 32 46 , , , cord-328082-7c7slfbp 32 47 further further RB cord-328082-7c7slfbp 32 48 linking link VBG cord-328082-7c7slfbp 32 49 mechanisms mechanism NNS cord-328082-7c7slfbp 32 50 of of IN cord-328082-7c7slfbp 32 51 signal signal NN cord-328082-7c7slfbp 32 52 transduction transduction NN cord-328082-7c7slfbp 32 53 and and CC cord-328082-7c7slfbp 32 54 the the DT cord-328082-7c7slfbp 32 55 endomembrane endomembrane NNP cord-328082-7c7slfbp 32 56 traffic traffic NN cord-328082-7c7slfbp 32 57 . . . cord-328082-7c7slfbp 33 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 33 2 A A NNP cord-328082-7c7slfbp 33 3 ( ( -LRB- cord-328082-7c7slfbp 33 4 BFA BFA NNP cord-328082-7c7slfbp 33 5 ) ) -RRB- cord-328082-7c7slfbp 33 6 ~ ~ NFP cord-328082-7c7slfbp 33 7 ( ( -LRB- cord-328082-7c7slfbp 33 8 Hiirri Hiirri NNP cord-328082-7c7slfbp 33 9 et et FW cord-328082-7c7slfbp 33 10 al al NNP cord-328082-7c7slfbp 33 11 . . NNP cord-328082-7c7slfbp 33 12 , , , cord-328082-7c7slfbp 33 13 1963 1963 CD cord-328082-7c7slfbp 33 14 ) ) -RRB- cord-328082-7c7slfbp 33 15 has have VBZ cord-328082-7c7slfbp 33 16 been be VBN cord-328082-7c7slfbp 33 17 a a DT cord-328082-7c7slfbp 33 18 frequently frequently RB cord-328082-7c7slfbp 33 19 used use VBN cord-328082-7c7slfbp 33 20 transport transport NN cord-328082-7c7slfbp 33 21 inhibitor inhibitor NN cord-328082-7c7slfbp 33 22 in in IN cord-328082-7c7slfbp 33 23 mammalian mammalian JJ cord-328082-7c7slfbp 33 24 cells cell NNS cord-328082-7c7slfbp 33 25 . . . cord-328082-7c7slfbp 34 1 BFA BFA NNP cord-328082-7c7slfbp 34 2 was be VBD cord-328082-7c7slfbp 34 3 first first RB cord-328082-7c7slfbp 34 4 reported report VBN cord-328082-7c7slfbp 34 5 to to TO cord-328082-7c7slfbp 34 6 block block VB cord-328082-7c7slfbp 34 7 the the DT cord-328082-7c7slfbp 34 8 transport transport NN cord-328082-7c7slfbp 34 9 of of IN cord-328082-7c7slfbp 34 10 vesicular vesicular JJ cord-328082-7c7slfbp 34 11 stomatitis stomatitis NN cord-328082-7c7slfbp 34 12 virus virus NN cord-328082-7c7slfbp 34 13 G g NN cord-328082-7c7slfbp 34 14 protein protein NN cord-328082-7c7slfbp 34 15 ( ( -LRB- cord-328082-7c7slfbp 34 16 Takatsuki Takatsuki NNP cord-328082-7c7slfbp 34 17 and and CC cord-328082-7c7slfbp 34 18 Tamura Tamura NNP cord-328082-7c7slfbp 34 19 , , , cord-328082-7c7slfbp 34 20 1985 1985 CD cord-328082-7c7slfbp 34 21 ) ) -RRB- cord-328082-7c7slfbp 34 22 . . . cord-328082-7c7slfbp 35 1 Later later RB cord-328082-7c7slfbp 35 2 it -PRON- PRP cord-328082-7c7slfbp 35 3 has have VBZ cord-328082-7c7slfbp 35 4 been be VBN cord-328082-7c7slfbp 35 5 shown show VBN cord-328082-7c7slfbp 35 6 that that IN cord-328082-7c7slfbp 35 7 BFA BFA NNP cord-328082-7c7slfbp 35 8 treatment treatment NN cord-328082-7c7slfbp 35 9 results result NNS cord-328082-7c7slfbp 35 10 in in IN cord-328082-7c7slfbp 35 11 the the DT cord-328082-7c7slfbp 35 12 fusion fusion NN cord-328082-7c7slfbp 35 13 of of IN cord-328082-7c7slfbp 35 14 the the DT cord-328082-7c7slfbp 35 15 Golgi Golgi NNP cord-328082-7c7slfbp 35 16 membranes membrane NNS cord-328082-7c7slfbp 35 17 with with IN cord-328082-7c7slfbp 35 18 the the DT cord-328082-7c7slfbp 35 19 ER er NN cord-328082-7c7slfbp 36 1 ( ( -LRB- cord-328082-7c7slfbp 36 2 Doms Doms NNP cord-328082-7c7slfbp 36 3 et et FW cord-328082-7c7slfbp 36 4 al al NNP cord-328082-7c7slfbp 36 5 . . . cord-328082-7c7slfbp 37 1 , , , cord-328082-7c7slfbp 37 2 1989 1989 CD cord-328082-7c7slfbp 37 3 ; ; : cord-328082-7c7slfbp 37 4 Lippincott Lippincott NNP cord-328082-7c7slfbp 37 5 - - HYPH cord-328082-7c7slfbp 37 6 Schwartz Schwartz NNP cord-328082-7c7slfbp 37 7 et et NNP cord-328082-7c7slfbp 37 8 al al NNP cord-328082-7c7slfbp 37 9 . . NNP cord-328082-7c7slfbp 37 10 , , , cord-328082-7c7slfbp 37 11 1989 1989 CD cord-328082-7c7slfbp 37 12 , , , cord-328082-7c7slfbp 37 13 1991a 1991a CD cord-328082-7c7slfbp 37 14 Strous Strous NNP cord-328082-7c7slfbp 37 15 et et NNP cord-328082-7c7slfbp 37 16 al al NNP cord-328082-7c7slfbp 37 17 . . NNP cord-328082-7c7slfbp 37 18 , , , cord-328082-7c7slfbp 37 19 1991 1991 CD cord-328082-7c7slfbp 37 20 ) ) -RRB- cord-328082-7c7slfbp 37 21 . . . cord-328082-7c7slfbp 38 1 The the DT cord-328082-7c7slfbp 38 2 almost almost RB cord-328082-7c7slfbp 38 3 immediate immediate JJ cord-328082-7c7slfbp 38 4 effect effect NN cord-328082-7c7slfbp 38 5 of of IN cord-328082-7c7slfbp 38 6 BFA BFA NNP cord-328082-7c7slfbp 38 7 is be VBZ cord-328082-7c7slfbp 38 8 to to TO cord-328082-7c7slfbp 38 9 detach detach VB cord-328082-7c7slfbp 38 10 at at RB cord-328082-7c7slfbp 38 11 least least RBS cord-328082-7c7slfbp 38 12 two two CD cord-328082-7c7slfbp 38 13 of of IN cord-328082-7c7slfbp 38 14 the the DT cord-328082-7c7slfbp 38 15 coat coat NN cord-328082-7c7slfbp 38 16 components-/3-COP components-/3-COP NNP cord-328082-7c7slfbp 38 17 ) ) -RRB- cord-328082-7c7slfbp 38 18 and and CC cord-328082-7c7slfbp 38 19 a a DT cord-328082-7c7slfbp 38 20 200-kD 200-kd CD cord-328082-7c7slfbp 38 21 protein protein NN cord-328082-7c7slfbp 38 22 ( ( -LRB- cord-328082-7c7slfbp 38 23 Narula Narula NNP cord-328082-7c7slfbp 38 24 et et NNP cord-328082-7c7slfbp 38 25 al al NNP cord-328082-7c7slfbp 38 26 . . NNP cord-328082-7c7slfbp 38 27 , , , cord-328082-7c7slfbp 38 28 1992 1992 CD cord-328082-7c7slfbp 38 29 ) ) -RRB- cord-328082-7c7slfbp 39 1 -from -from JJ cord-328082-7c7slfbp 40 1 the the DT cord-328082-7c7slfbp 40 2 Golgi Golgi NNP cord-328082-7c7slfbp 40 3 stack stack NN cord-328082-7c7slfbp 40 4 , , , cord-328082-7c7slfbp 40 5 allowing allow VBG cord-328082-7c7slfbp 40 6 Golgi Golgi NNP cord-328082-7c7slfbp 40 7 membranes membrane NNS cord-328082-7c7slfbp 40 8 to to TO cord-328082-7c7slfbp 40 9 form form VB cord-328082-7c7slfbp 40 10 tubular tubular JJ cord-328082-7c7slfbp 40 11 extensions extension NNS cord-328082-7c7slfbp 40 12 along along IN cord-328082-7c7slfbp 40 13 microtubules microtubule NNS cord-328082-7c7slfbp 40 14 that that WDT cord-328082-7c7slfbp 40 15 finally finally RB cord-328082-7c7slfbp 40 16 fuse fuse VBP cord-328082-7c7slfbp 40 17 with with IN cord-328082-7c7slfbp 40 18 the the DT cord-328082-7c7slfbp 40 19 ER ER NNP cord-328082-7c7slfbp 40 20 . . . cord-328082-7c7slfbp 41 1 This this DT cord-328082-7c7slfbp 41 2 effect effect NN cord-328082-7c7slfbp 41 3 of of IN cord-328082-7c7slfbp 41 4 BFA BFA NNP cord-328082-7c7slfbp 41 5 has have VBZ cord-328082-7c7slfbp 41 6 been be VBN cord-328082-7c7slfbp 41 7 proposed propose VBN cord-328082-7c7slfbp 41 8 to to TO cord-328082-7c7slfbp 41 9 reveal reveal VB cord-328082-7c7slfbp 41 10 a a DT cord-328082-7c7slfbp 41 11 normally normally RB cord-328082-7c7slfbp 41 12 occurring occur VBG cord-328082-7c7slfbp 41 13 retrograde retrograde JJ cord-328082-7c7slfbp 41 14 membrane membrane NN cord-328082-7c7slfbp 41 15 traffic traffic NN cord-328082-7c7slfbp 41 16 from from IN cord-328082-7c7slfbp 41 17 the the DT cord-328082-7c7slfbp 41 18 Golgi Golgi NNP cord-328082-7c7slfbp 41 19 apparatus apparatus NN cord-328082-7c7slfbp 41 20 to to IN cord-328082-7c7slfbp 41 21 the the DT cord-328082-7c7slfbp 41 22 ER er NN cord-328082-7c7slfbp 42 1 ( ( -LRB- cord-328082-7c7slfbp 42 2 Doms Doms NNP cord-328082-7c7slfbp 42 3 et et FW cord-328082-7c7slfbp 42 4 al al NNP cord-328082-7c7slfbp 42 5 . . . cord-328082-7c7slfbp 43 1 , , , cord-328082-7c7slfbp 43 2 1989 1989 CD cord-328082-7c7slfbp 43 3 ; ; : cord-328082-7c7slfbp 43 4 Lippincott Lippincott NNP cord-328082-7c7slfbp 43 5 - - HYPH cord-328082-7c7slfbp 43 6 Schwartz Schwartz NNP cord-328082-7c7slfbp 43 7 et et NNP cord-328082-7c7slfbp 43 8 al al NNP cord-328082-7c7slfbp 43 9 . . . cord-328082-7c7slfbp 44 1 , , , cord-328082-7c7slfbp 44 2 1989 1989 CD cord-328082-7c7slfbp 44 3 ; ; : cord-328082-7c7slfbp 44 4 Klausner Klausner NNP cord-328082-7c7slfbp 44 5 et et NNP cord-328082-7c7slfbp 44 6 al al NNP cord-328082-7c7slfbp 44 7 . . NNP cord-328082-7c7slfbp 44 8 , , , cord-328082-7c7slfbp 44 9 1992 1992 CD cord-328082-7c7slfbp 44 10 ) ) -RRB- cord-328082-7c7slfbp 44 11 . . . cord-328082-7c7slfbp 45 1 BFA BFA NNP cord-328082-7c7slfbp 45 2 has have VBZ cord-328082-7c7slfbp 45 3 also also RB cord-328082-7c7slfbp 45 4 been be VBN cord-328082-7c7slfbp 45 5 reported report VBN cord-328082-7c7slfbp 45 6 to to TO cord-328082-7c7slfbp 45 7 have have VB cord-328082-7c7slfbp 45 8 effects effect NNS cord-328082-7c7slfbp 45 9 on on IN cord-328082-7c7slfbp 45 10 the the DT cord-328082-7c7slfbp 45 11 organization organization NN cord-328082-7c7slfbp 45 12 of of IN cord-328082-7c7slfbp 45 13 the the DT cord-328082-7c7slfbp 45 14 endocytic endocytic JJ cord-328082-7c7slfbp 45 15 compartment compartment NN cord-328082-7c7slfbp 45 16 ; ; : cord-328082-7c7slfbp 45 17 TGN TGN NNP cord-328082-7c7slfbp 45 18 and and CC cord-328082-7c7slfbp 45 19 endosomes endosome NNS cord-328082-7c7slfbp 45 20 form form VBP cord-328082-7c7slfbp 45 21 large large JJ cord-328082-7c7slfbp 45 22 tubular tubular JJ cord-328082-7c7slfbp 45 23 structures structure NNS cord-328082-7c7slfbp 45 24 after after IN cord-328082-7c7slfbp 45 25 a a DT cord-328082-7c7slfbp 45 26 BFA BFA NNP cord-328082-7c7slfbp 45 27 treatment treatment NN cord-328082-7c7slfbp 45 28 ( ( -LRB- cord-328082-7c7slfbp 45 29 Wood Wood NNP cord-328082-7c7slfbp 45 30 et et FW cord-328082-7c7slfbp 45 31 al al NNP cord-328082-7c7slfbp 45 32 . . NNP cord-328082-7c7slfbp 45 33 , , , cord-328082-7c7slfbp 45 34 1991 1991 CD cord-328082-7c7slfbp 45 35 ; ; : cord-328082-7c7slfbp 46 1 Lippincott Lippincott NNP cord-328082-7c7slfbp 46 2 - - HYPH cord-328082-7c7slfbp 46 3 Schwartz Schwartz NNP cord-328082-7c7slfbp 46 4 et et NNP cord-328082-7c7slfbp 46 5 al al NNP cord-328082-7c7slfbp 46 6 . . . cord-328082-7c7slfbp 47 1 , , , cord-328082-7c7slfbp 47 2 1991b 1991b CD cord-328082-7c7slfbp 47 3 ; ; : cord-328082-7c7slfbp 47 4 Reaves Reaves NNP cord-328082-7c7slfbp 47 5 and and CC cord-328082-7c7slfbp 47 6 Banting Banting NNP cord-328082-7c7slfbp 47 7 , , , cord-328082-7c7slfbp 47 8 1992 1992 CD cord-328082-7c7slfbp 47 9 ) ) -RRB- cord-328082-7c7slfbp 47 10 . . . cord-328082-7c7slfbp 48 1 We -PRON- PRP cord-328082-7c7slfbp 48 2 have have VBP cord-328082-7c7slfbp 48 3 previously previously RB cord-328082-7c7slfbp 48 4 shown show VBN cord-328082-7c7slfbp 48 5 that that IN cord-328082-7c7slfbp 48 6 10 10 CD cord-328082-7c7slfbp 48 7 mM mm CD cord-328082-7c7slfbp 48 8 caffeine caffeine NN cord-328082-7c7slfbp 48 9 affects affect VBZ cord-328082-7c7slfbp 48 10 the the DT cord-328082-7c7slfbp 48 11 intracellular intracellular JJ cord-328082-7c7slfbp 48 12 transport transport NN cord-328082-7c7slfbp 48 13 of of IN cord-328082-7c7slfbp 48 14 Semliki Semliki NNP cord-328082-7c7slfbp 48 15 Forest Forest NNP cord-328082-7c7slfbp 48 16 virus virus NN cord-328082-7c7slfbp 48 17 ( ( -LRB- cord-328082-7c7slfbp 48 18 SFV sfv NN cord-328082-7c7slfbp 48 19 ) ) -RRB- cord-328082-7c7slfbp 48 20 membrane membrane NN cord-328082-7c7slfbp 48 21 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 48 22 . . . cord-328082-7c7slfbp 49 1 In in IN cord-328082-7c7slfbp 49 2 this this DT cord-328082-7c7slfbp 49 3 study study NN cord-328082-7c7slfbp 49 4 we -PRON- PRP cord-328082-7c7slfbp 49 5 have have VBP cord-328082-7c7slfbp 49 6 characterized characterize VBN cord-328082-7c7slfbp 49 7 the the DT cord-328082-7c7slfbp 49 8 temperature temperature NN cord-328082-7c7slfbp 49 9 - - HYPH cord-328082-7c7slfbp 49 10 dependent dependent JJ cord-328082-7c7slfbp 49 11 effect effect NN cord-328082-7c7slfbp 49 12 of of IN cord-328082-7c7slfbp 49 13 caffeine caffeine NN cord-328082-7c7slfbp 49 14 on on IN cord-328082-7c7slfbp 49 15 the the DT cord-328082-7c7slfbp 49 16 distribution distribution NN cord-328082-7c7slfbp 49 17 and and CC cord-328082-7c7slfbp 49 18 organization organization NN cord-328082-7c7slfbp 49 19 of of IN cord-328082-7c7slfbp 49 20 the the DT cord-328082-7c7slfbp 49 21 Golgi Golgi NNP cord-328082-7c7slfbp 49 22 complex complex JJ cord-328082-7c7slfbp 49 23 membranes membrane NNS cord-328082-7c7slfbp 49 24 . . . cord-328082-7c7slfbp 50 1 At at IN cord-328082-7c7slfbp 50 2 20~ 20~ CD cord-328082-7c7slfbp 50 3 caffeine caffeine NN cord-328082-7c7slfbp 50 4 is be VBZ cord-328082-7c7slfbp 50 5 shown show VBN cord-328082-7c7slfbp 50 6 to to TO cord-328082-7c7slfbp 50 7 translocate translocate VB cord-328082-7c7slfbp 50 8 p58 p58 NNP cord-328082-7c7slfbp 50 9 , , , cord-328082-7c7slfbp 50 10 a a DT cord-328082-7c7slfbp 50 11 marker marker NN cord-328082-7c7slfbp 50 12 for for IN cord-328082-7c7slfbp 50 13 the the DT cord-328082-7c7slfbp 50 14 pre pre JJ cord-328082-7c7slfbp 50 15 - - JJ cord-328082-7c7slfbp 50 16 and and CC cord-328082-7c7slfbp 50 17 cis cis NNP cord-328082-7c7slfbp 50 18 - - HYPH cord-328082-7c7slfbp 50 19 Golgi Golgi NNP cord-328082-7c7slfbp 50 20 compartments compartment NNS cord-328082-7c7slfbp 50 21 , , , cord-328082-7c7slfbp 50 22 to to IN cord-328082-7c7slfbp 50 23 the the DT cord-328082-7c7slfbp 50 24 periphery periphery NN cord-328082-7c7slfbp 50 25 of of IN cord-328082-7c7slfbp 50 26 the the DT cord-328082-7c7slfbp 50 27 cell cell NN cord-328082-7c7slfbp 50 28 . . . cord-328082-7c7slfbp 51 1 In in IN cord-328082-7c7slfbp 51 2 contrast contrast NN cord-328082-7c7slfbp 51 3 , , , cord-328082-7c7slfbp 51 4 under under IN cord-328082-7c7slfbp 51 5 the the DT cord-328082-7c7slfbp 51 6 same same JJ cord-328082-7c7slfbp 51 7 conditions condition NNS cord-328082-7c7slfbp 51 8 a a DT cord-328082-7c7slfbp 51 9 Golgi Golgi NNP cord-328082-7c7slfbp 51 10 stack stack NN cord-328082-7c7slfbp 51 11 marker marker NN cord-328082-7c7slfbp 51 12 , , , cord-328082-7c7slfbp 51 13 mannosidase mannosidase NN cord-328082-7c7slfbp 51 14 II II NNP cord-328082-7c7slfbp 51 15 ( ( -LRB- cord-328082-7c7slfbp 51 16 man man NN cord-328082-7c7slfbp 51 17 II II NNP cord-328082-7c7slfbp 51 18 ) ) -RRB- cord-328082-7c7slfbp 51 19 , , , cord-328082-7c7slfbp 51 20 remains remain VBZ cord-328082-7c7slfbp 51 21 perinuclearly perinuclearly RB cord-328082-7c7slfbp 51 22 located locate VBN cord-328082-7c7slfbp 51 23 . . . cord-328082-7c7slfbp 52 1 The the DT cord-328082-7c7slfbp 52 2 redistribution redistribution NN cord-328082-7c7slfbp 52 3 of of IN cord-328082-7c7slfbp 52 4 membranes membrane NNS cord-328082-7c7slfbp 52 5 induced induce VBN cord-328082-7c7slfbp 52 6 by by IN cord-328082-7c7slfbp 52 7 caffeine caffeine NN cord-328082-7c7slfbp 52 8 is be VBZ cord-328082-7c7slfbp 52 9 clearly clearly RB cord-328082-7c7slfbp 52 10 different different JJ cord-328082-7c7slfbp 52 11 from from IN cord-328082-7c7slfbp 52 12 that that DT cord-328082-7c7slfbp 52 13 induced induce VBN cord-328082-7c7slfbp 52 14 by by IN cord-328082-7c7slfbp 52 15 BFA BFA NNP cord-328082-7c7slfbp 52 16 . . . cord-328082-7c7slfbp 53 1 Further further RB cord-328082-7c7slfbp 53 2 , , , cord-328082-7c7slfbp 53 3 caffeine caffeine NN cord-328082-7c7slfbp 53 4 at at IN cord-328082-7c7slfbp 53 5 reduced reduce VBN cord-328082-7c7slfbp 53 6 temperature temperature NN cord-328082-7c7slfbp 53 7 did do VBD cord-328082-7c7slfbp 53 8 not not RB cord-328082-7c7slfbp 53 9 inhibit inhibit VB cord-328082-7c7slfbp 53 10 the the DT cord-328082-7c7slfbp 53 11 retrograde retrograde JJ cord-328082-7c7slfbp 53 12 movement movement NN cord-328082-7c7slfbp 53 13 of of IN cord-328082-7c7slfbp 53 14 Golgi Golgi NNP cord-328082-7c7slfbp 53 15 stack stack NN cord-328082-7c7slfbp 53 16 membranes membrane NNS cord-328082-7c7slfbp 53 17 observed observe VBN cord-328082-7c7slfbp 53 18 in in IN cord-328082-7c7slfbp 53 19 BFA BFA NNP cord-328082-7c7slfbp 53 20 - - HYPH cord-328082-7c7slfbp 53 21 treated treat VBN cord-328082-7c7slfbp 53 22 cells cell NNS cord-328082-7c7slfbp 53 23 . . . cord-328082-7c7slfbp 54 1 Taken take VBN cord-328082-7c7slfbp 54 2 together together RB cord-328082-7c7slfbp 54 3 the the DT cord-328082-7c7slfbp 54 4 results result NNS cord-328082-7c7slfbp 54 5 in in IN cord-328082-7c7slfbp 54 6 the the DT cord-328082-7c7slfbp 54 7 present present JJ cord-328082-7c7slfbp 54 8 study study NN cord-328082-7c7slfbp 54 9 suggest suggest VBP cord-328082-7c7slfbp 54 10 that that IN cord-328082-7c7slfbp 54 11 10 10 CD cord-328082-7c7slfbp 54 12 mM mm CD cord-328082-7c7slfbp 54 13 caffeine caffeine NN cord-328082-7c7slfbp 54 14 at at IN cord-328082-7c7slfbp 54 15 reduced reduce VBN cord-328082-7c7slfbp 54 16 temperature temperature NN cord-328082-7c7slfbp 54 17 can can MD cord-328082-7c7slfbp 54 18 be be VB cord-328082-7c7slfbp 54 19 used use VBN cord-328082-7c7slfbp 54 20 to to TO cord-328082-7c7slfbp 54 21 dissect dissect VB cord-328082-7c7slfbp 54 22 the the DT cord-328082-7c7slfbp 54 23 interface interface NN cord-328082-7c7slfbp 54 24 between between IN cord-328082-7c7slfbp 54 25 the the DT cord-328082-7c7slfbp 54 26 cis cis NNP cord-328082-7c7slfbp 54 27 - - HYPH cord-328082-7c7slfbp 54 28 Golgi Golgi NNP cord-328082-7c7slfbp 54 29 and and CC cord-328082-7c7slfbp 54 30 the the DT cord-328082-7c7slfbp 54 31 Golgi Golgi NNP cord-328082-7c7slfbp 54 32 stack stack NN cord-328082-7c7slfbp 54 33 . . . cord-328082-7c7slfbp 54 34 _SP cord-328082-7c7slfbp 55 1 The the DT cord-328082-7c7slfbp 55 2 antibodies antibody NNS cord-328082-7c7slfbp 55 3 were be VBD cord-328082-7c7slfbp 55 4 kind kind JJ cord-328082-7c7slfbp 55 5 gifts gift NNS cord-328082-7c7slfbp 55 6 from from IN cord-328082-7c7slfbp 55 7 the the DT cord-328082-7c7slfbp 55 8 following follow VBG cord-328082-7c7slfbp 55 9 persons person NNS cord-328082-7c7slfbp 55 10 : : : cord-328082-7c7slfbp 55 11 polyclonal polyclonal JJ cord-328082-7c7slfbp 55 12 antibodies antibody NNS cord-328082-7c7slfbp 55 13 to to IN cord-328082-7c7slfbp 55 14 / / SYM cord-328082-7c7slfbp 55 15 S s NN cord-328082-7c7slfbp 55 16 - - HYPH cord-328082-7c7slfbp 55 17 COP COP NNP cord-328082-7c7slfbp 55 18 ( ( -LRB- cord-328082-7c7slfbp 55 19 EAGE EAGE NNP cord-328082-7c7slfbp 55 20 ) ) -RRB- cord-328082-7c7slfbp 56 1 ( ( -LRB- cord-328082-7c7slfbp 56 2 Duden Duden NNP cord-328082-7c7slfbp 56 3 et et FW cord-328082-7c7slfbp 56 4 al al NNP cord-328082-7c7slfbp 56 5 . . NNP cord-328082-7c7slfbp 56 6 , , , cord-328082-7c7slfbp 56 7 1991 1991 CD cord-328082-7c7slfbp 56 8 ) ) -RRB- cord-328082-7c7slfbp 56 9 were be VBD cord-328082-7c7slfbp 56 10 from from IN cord-328082-7c7slfbp 56 11 Drs Drs NNP cord-328082-7c7slfbp 56 12 . . . cord-328082-7c7slfbp 57 1 R. R. NNP cord-328082-7c7slfbp 57 2 Duden Duden NNP cord-328082-7c7slfbp 57 3 ( ( -LRB- cord-328082-7c7slfbp 57 4 EMBL EMBL NNP cord-328082-7c7slfbp 57 5 , , , cord-328082-7c7slfbp 57 6 Heidelberg Heidelberg NNP cord-328082-7c7slfbp 57 7 , , , cord-328082-7c7slfbp 57 8 Germany Germany NNP cord-328082-7c7slfbp 57 9 ) ) -RRB- cord-328082-7c7slfbp 57 10 and and CC cord-328082-7c7slfbp 57 11 T. T. NNP cord-328082-7c7slfbp 57 12 Kreis Kreis NNP cord-328082-7c7slfbp 57 13 ( ( -LRB- cord-328082-7c7slfbp 57 14 University University NNP cord-328082-7c7slfbp 57 15 of of IN cord-328082-7c7slfbp 57 16 Geneva Geneva NNP cord-328082-7c7slfbp 57 17 , , , cord-328082-7c7slfbp 57 18 Geneva Geneva NNP cord-328082-7c7slfbp 57 19 , , , cord-328082-7c7slfbp 57 20 Switzerland Switzerland NNP cord-328082-7c7slfbp 57 21 ) ) -RRB- cord-328082-7c7slfbp 57 22 ; ; , cord-328082-7c7slfbp 57 23 polyclonal polyclonal JJ cord-328082-7c7slfbp 57 24 antibodies antibody NNS cord-328082-7c7slfbp 57 25 to to TO cord-328082-7c7slfbp 57 26 man man VB cord-328082-7c7slfbp 57 27 II II NNP cord-328082-7c7slfbp 57 28 were be VBD cord-328082-7c7slfbp 57 29 from from IN cord-328082-7c7slfbp 57 30 Drs Drs NNP cord-328082-7c7slfbp 57 31 . . . cord-328082-7c7slfbp 58 1 M. M. NNP cord-328082-7c7slfbp 58 2 Farquhar Farquhar NNP cord-328082-7c7slfbp 58 3 ( ( -LRB- cord-328082-7c7slfbp 58 4 University University NNP cord-328082-7c7slfbp 58 5 of of IN cord-328082-7c7slfbp 58 6 California California NNP cord-328082-7c7slfbp 58 7 , , , cord-328082-7c7slfbp 58 8 San San NNP cord-328082-7c7slfbp 58 9 Diego Diego NNP cord-328082-7c7slfbp 58 10 , , , cord-328082-7c7slfbp 58 11 CA CA NNP cord-328082-7c7slfbp 58 12 ) ) -RRB- cord-328082-7c7slfbp 58 13 and and CC cord-328082-7c7slfbp 58 14 K. K. NNP cord-328082-7c7slfbp 58 15 Moremen Moremen NNP cord-328082-7c7slfbp 58 16 ( ( -LRB- cord-328082-7c7slfbp 58 17 Massachusetts Massachusetts NNP cord-328082-7c7slfbp 58 18 Institute Institute NNP cord-328082-7c7slfbp 58 19 of of IN cord-328082-7c7slfbp 58 20 Technology Technology NNP cord-328082-7c7slfbp 58 21 , , , cord-328082-7c7slfbp 58 22 Cambridge Cambridge NNP cord-328082-7c7slfbp 58 23 , , , cord-328082-7c7slfbp 58 24 MA MA NNP cord-328082-7c7slfbp 58 25 ) ) -RRB- cord-328082-7c7slfbp 58 26 ; ; , cord-328082-7c7slfbp 58 27 monoclonal monoclonal JJ cord-328082-7c7slfbp 58 28 antibodies antibody NNS cord-328082-7c7slfbp 58 29 to to TO cord-328082-7c7slfbp 58 30 man man VB cord-328082-7c7slfbp 58 31 II II NNP cord-328082-7c7slfbp 58 32 ( ( -LRB- cord-328082-7c7slfbp 58 33 135-kD 135-kd CD cord-328082-7c7slfbp 58 34 protein protein NN cord-328082-7c7slfbp 58 35 ) ) -RRB- cord-328082-7c7slfbp 58 36 were be VBD cord-328082-7c7slfbp 58 37 from from IN cord-328082-7c7slfbp 58 38 Drs Drs NNP cord-328082-7c7slfbp 58 39 . . . cord-328082-7c7slfbp 59 1 B. B. NNP cord-328082-7c7slfbp 59 2 Burke Burke NNP cord-328082-7c7slfbp 59 3 ( ( -LRB- cord-328082-7c7slfbp 59 4 Harvard Harvard NNP cord-328082-7c7slfbp 59 5 Medical Medical NNP cord-328082-7c7slfbp 59 6 School School NNP cord-328082-7c7slfbp 59 7 , , , cord-328082-7c7slfbp 59 8 Boston Boston NNP cord-328082-7c7slfbp 59 9 , , , cord-328082-7c7slfbp 59 10 MA MA NNP cord-328082-7c7slfbp 59 11 ) ) -RRB- cord-328082-7c7slfbp 59 12 and and CC cord-328082-7c7slfbp 59 13 G. G. NNP cord-328082-7c7slfbp 59 14 Warren Warren NNP cord-328082-7c7slfbp 59 15 ( ( -LRB- cord-328082-7c7slfbp 59 16 Imperial Imperial NNP cord-328082-7c7slfbp 59 17 Cancer Cancer NNP cord-328082-7c7slfbp 59 18 Research Research NNP cord-328082-7c7slfbp 59 19 Fund Fund NNP cord-328082-7c7slfbp 59 20 , , , cord-328082-7c7slfbp 59 21 London London NNP cord-328082-7c7slfbp 59 22 , , , cord-328082-7c7slfbp 59 23 UK UK NNP cord-328082-7c7slfbp 59 24 ) ) -RRB- cord-328082-7c7slfbp 60 1 ( ( -LRB- cord-328082-7c7slfbp 60 2 Burke Burke NNP cord-328082-7c7slfbp 60 3 et et NNP cord-328082-7c7slfbp 60 4 al al NNP cord-328082-7c7slfbp 60 5 . . NNP cord-328082-7c7slfbp 60 6 , , , cord-328082-7c7slfbp 60 7 1982 1982 CD cord-328082-7c7slfbp 60 8 ) ) -RRB- cord-328082-7c7slfbp 60 9 ; ; , cord-328082-7c7slfbp 60 10 polyclonal polyclonal JJ cord-328082-7c7slfbp 60 11 antibodies antibody NNS cord-328082-7c7slfbp 60 12 to to IN cord-328082-7c7slfbp 60 13 p58 p58 NNP cord-328082-7c7slfbp 60 14 were be VBD cord-328082-7c7slfbp 60 15 from from IN cord-328082-7c7slfbp 60 16 Dr. Dr. NNP cord-328082-7c7slfbp 60 17 J. J. NNP cord-328082-7c7slfbp 60 18 Saraste Saraste NNP cord-328082-7c7slfbp 60 19 ( ( -LRB- cord-328082-7c7slfbp 60 20 University University NNP cord-328082-7c7slfbp 60 21 of of IN cord-328082-7c7slfbp 60 22 Bergen Bergen NNP cord-328082-7c7slfbp 60 23 , , , cord-328082-7c7slfbp 60 24 Bergen Bergen NNP cord-328082-7c7slfbp 60 25 , , , cord-328082-7c7slfbp 60 26 Norway Norway NNP cord-328082-7c7slfbp 60 27 ) ) -RRB- cord-328082-7c7slfbp 60 28 ( ( -LRB- cord-328082-7c7slfbp 60 29 Saraste Saraste NNP cord-328082-7c7slfbp 60 30 and and CC cord-328082-7c7slfbp 60 31 Svensson Svensson NNP cord-328082-7c7slfbp 60 32 , , , cord-328082-7c7slfbp 60 33 1991 1991 CD cord-328082-7c7slfbp 60 34 ) ) -RRB- cord-328082-7c7slfbp 60 35 ; ; : cord-328082-7c7slfbp 60 36 and and CC cord-328082-7c7slfbp 60 37 polyclonal polyclonal JJ cord-328082-7c7slfbp 60 38 antibodies antibody NNS cord-328082-7c7slfbp 60 39 to to IN cord-328082-7c7slfbp 60 40 SFV sfv NN cord-328082-7c7slfbp 60 41 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 60 42 El El NNP cord-328082-7c7slfbp 60 43 and and CC cord-328082-7c7slfbp 60 44 E2 E2 NNP cord-328082-7c7slfbp 60 45 were be VBD cord-328082-7c7slfbp 60 46 from from IN cord-328082-7c7slfbp 60 47 Dr. Dr. NNP cord-328082-7c7slfbp 60 48 J. J. NNP cord-328082-7c7slfbp 60 49 Pe Pe NNP cord-328082-7c7slfbp 60 50 ~ ~ NFP cord-328082-7c7slfbp 60 51 nen nen NNP cord-328082-7c7slfbp 60 52 ( ( -LRB- cord-328082-7c7slfbp 60 53 University University NNP cord-328082-7c7slfbp 60 54 of of IN cord-328082-7c7slfbp 60 55 Helsinki Helsinki NNP cord-328082-7c7slfbp 60 56 , , , cord-328082-7c7slfbp 60 57 Helsinki Helsinki NNP cord-328082-7c7slfbp 60 58 , , , cord-328082-7c7slfbp 60 59 Finland Finland NNP cord-328082-7c7slfbp 60 60 ) ) -RRB- cord-328082-7c7slfbp 60 61 . . . cord-328082-7c7slfbp 61 1 TRITC TRITC NNP cord-328082-7c7slfbp 61 2 - - HYPH cord-328082-7c7slfbp 61 3 and and CC cord-328082-7c7slfbp 61 4 FITC FITC NNP cord-328082-7c7slfbp 61 5 - - HYPH cord-328082-7c7slfbp 61 6 conjugated conjugate VBN cord-328082-7c7slfbp 61 7 secondary secondary JJ cord-328082-7c7slfbp 61 8 antibodies antibody NNS cord-328082-7c7slfbp 61 9 to to IN cord-328082-7c7slfbp 61 10 rabbit rabbit NN cord-328082-7c7slfbp 61 11 or or CC cord-328082-7c7slfbp 61 12 mouse mouse NN cord-328082-7c7slfbp 61 13 IgG IgG NNP cord-328082-7c7slfbp 61 14 were be VBD cord-328082-7c7slfbp 61 15 purchased purchase VBN cord-328082-7c7slfbp 61 16 from from IN cord-328082-7c7slfbp 61 17 Dako Dako NNP cord-328082-7c7slfbp 61 18 A A NNP cord-328082-7c7slfbp 61 19 / / SYM cord-328082-7c7slfbp 61 20 S S NNP cord-328082-7c7slfbp 61 21 , , , cord-328082-7c7slfbp 61 22 Glostrup Glostrup NNP cord-328082-7c7slfbp 61 23 , , , cord-328082-7c7slfbp 61 24 Denmark Denmark NNP cord-328082-7c7slfbp 61 25 and and CC cord-328082-7c7slfbp 61 26 peroxidaseconjugated peroxidaseconjugate VBN cord-328082-7c7slfbp 61 27 Fab fab JJ cord-328082-7c7slfbp 61 28 fragments fragment NNS cord-328082-7c7slfbp 61 29 to to IN cord-328082-7c7slfbp 61 30 rabbit rabbit NN cord-328082-7c7slfbp 61 31 IgG IgG NNP cord-328082-7c7slfbp 61 32 were be VBD cord-328082-7c7slfbp 61 33 purchased purchase VBN cord-328082-7c7slfbp 61 34 from from IN cord-328082-7c7slfbp 61 35 Biosys Biosys NNP cord-328082-7c7slfbp 61 36 , , , cord-328082-7c7slfbp 61 37 Compiegne Compiegne NNP cord-328082-7c7slfbp 61 38 , , , cord-328082-7c7slfbp 61 39 France France NNP cord-328082-7c7slfbp 61 40 . . . cord-328082-7c7slfbp 62 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 63 1 cells cell NNS cord-328082-7c7slfbp 63 2 were be VBD cord-328082-7c7slfbp 63 3 grown grow VBN cord-328082-7c7slfbp 63 4 on on IN cord-328082-7c7slfbp 63 5 35-mm 35-mm CD cord-328082-7c7slfbp 63 6 - - HYPH cord-328082-7c7slfbp 63 7 diam diam NN cord-328082-7c7slfbp 63 8 tissue tissue NN cord-328082-7c7slfbp 63 9 culture culture NN cord-328082-7c7slfbp 63 10 dishes dish NNS cord-328082-7c7slfbp 63 11 in in IN cord-328082-7c7slfbp 63 12 DMEM DMEM NNP cord-328082-7c7slfbp 64 1 ( ( -LRB- cord-328082-7c7slfbp 64 2 Gibeo Gibeo NNP cord-328082-7c7slfbp 64 3 BRL BRL NNP cord-328082-7c7slfbp 64 4 , , , cord-328082-7c7slfbp 64 5 Roskilde Roskilde NNP cord-328082-7c7slfbp 64 6 , , , cord-328082-7c7slfbp 64 7 Denmark Denmark NNP cord-328082-7c7slfbp 64 8 ) ) -RRB- cord-328082-7c7slfbp 64 9 in in IN cord-328082-7c7slfbp 64 10 the the DT cord-328082-7c7slfbp 64 11 presence presence NN cord-328082-7c7slfbp 64 12 of of IN cord-328082-7c7slfbp 64 13 10 10 CD cord-328082-7c7slfbp 64 14 % % NN cord-328082-7c7slfbp 64 15 FCS FCS NNP cord-328082-7c7slfbp 64 16 ( ( -LRB- cord-328082-7c7slfbp 64 17 Gibeo Gibeo NNP cord-328082-7c7slfbp 64 18 BRL BRL NNP cord-328082-7c7slfbp 64 19 ) ) -RRB- cord-328082-7c7slfbp 64 20 , , , cord-328082-7c7slfbp 64 21 100 100 CD cord-328082-7c7slfbp 64 22 IU IU NNP cord-328082-7c7slfbp 64 23 / / SYM cord-328082-7c7slfbp 64 24 mi mi NNP cord-328082-7c7slfbp 64 25 of of IN cord-328082-7c7slfbp 64 26 penicillin penicillin NNP cord-328082-7c7slfbp 64 27 , , , cord-328082-7c7slfbp 64 28 and and CC cord-328082-7c7slfbp 64 29 100 100 CD cord-328082-7c7slfbp 64 30 # # NN cord-328082-7c7slfbp 64 31 g g NN cord-328082-7c7slfbp 64 32 / / SYM cord-328082-7c7slfbp 64 33 ml ml NN cord-328082-7c7slfbp 64 34 of of IN cord-328082-7c7slfbp 64 35 streptomycin streptomycin NNS cord-328082-7c7slfbp 64 36 ( ( -LRB- cord-328082-7c7slfbp 64 37 Penstrep Penstrep NNP cord-328082-7c7slfbp 64 38 ) ) -RRB- cord-328082-7c7slfbp 64 39 ( ( -LRB- cord-328082-7c7slfbp 64 40 Gibco Gibco NNP cord-328082-7c7slfbp 64 41 BRL BRL NNP cord-328082-7c7slfbp 64 42 ) ) -RRB- cord-328082-7c7slfbp 64 43 . . . cord-328082-7c7slfbp 65 1 For for IN cord-328082-7c7slfbp 65 2 immunofiuorescence immunofiuorescence NN cord-328082-7c7slfbp 65 3 experiments experiment NNS cord-328082-7c7slfbp 65 4 the the DT cord-328082-7c7slfbp 65 5 cells cell NNS cord-328082-7c7slfbp 65 6 were be VBD cord-328082-7c7slfbp 65 7 grown grow VBN cord-328082-7c7slfbp 65 8 on on IN cord-328082-7c7slfbp 65 9 12-mm 12-mm CD cord-328082-7c7slfbp 65 10 - - HYPH cord-328082-7c7slfbp 65 11 diam diam NN cord-328082-7c7slfbp 65 12 microscope microscope NN cord-328082-7c7slfbp 65 13 coverglasses coverglasse NNS cord-328082-7c7slfbp 65 14 . . . cord-328082-7c7slfbp 66 1 Incubations incubation NNS cord-328082-7c7slfbp 66 2 in in IN cord-328082-7c7slfbp 66 3 temperatures temperature NNS cord-328082-7c7slfbp 66 4 other other JJ cord-328082-7c7slfbp 66 5 than than IN cord-328082-7c7slfbp 66 6 37~ 37~ CD cord-328082-7c7slfbp 66 7 were be VBD cord-328082-7c7slfbp 66 8 carried carry VBN cord-328082-7c7slfbp 66 9 out out RP cord-328082-7c7slfbp 66 10 in in IN cord-328082-7c7slfbp 66 11 water water NN cord-328082-7c7slfbp 66 12 baths bath NNS cord-328082-7c7slfbp 66 13 in in IN cord-328082-7c7slfbp 66 14 NaHCO3-free NaHCO3-free NNP cord-328082-7c7slfbp 66 15 MEM MEM NNP cord-328082-7c7slfbp 66 16 ( ( -LRB- cord-328082-7c7slfbp 66 17 Gibeo Gibeo NNP cord-328082-7c7slfbp 66 18 BRL BRL NNP cord-328082-7c7slfbp 66 19 ) ) -RRB- cord-328082-7c7slfbp 66 20 supplemented supplement VBD cord-328082-7c7slfbp 66 21 with with IN cord-328082-7c7slfbp 66 22 20 20 CD cord-328082-7c7slfbp 66 23 mM mM NNP cord-328082-7c7slfbp 66 24 Hepes Hepes NNP cord-328082-7c7slfbp 66 25 and and CC cord-328082-7c7slfbp 66 26 Penstrep Penstrep NNP cord-328082-7c7slfbp 66 27 as as IN cord-328082-7c7slfbp 66 28 described describe VBN cord-328082-7c7slfbp 66 29 earlier early RBR cord-328082-7c7slfbp 66 30 ( ( -LRB- cord-328082-7c7slfbp 66 31 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 66 32 and and CC cord-328082-7c7slfbp 66 33 Saraste Saraste NNP cord-328082-7c7slfbp 66 34 , , , cord-328082-7c7slfbp 66 35 1989 1989 CD cord-328082-7c7slfbp 66 36 ) ) -RRB- cord-328082-7c7slfbp 66 37 . . . cord-328082-7c7slfbp 67 1 The the DT cord-328082-7c7slfbp 67 2 temperature temperature NN cord-328082-7c7slfbp 67 3 - - HYPH cord-328082-7c7slfbp 67 4 sensitive sensitive JJ cord-328082-7c7slfbp 67 5 mutant mutant JJ cord-328082-7c7slfbp 67 6 tsq tsq NN cord-328082-7c7slfbp 67 7 ofSFV ofsfv UH cord-328082-7c7slfbp 68 1 ( ( -LRB- cord-328082-7c7slfbp 68 2 SFV SFV NNP cord-328082-7c7slfbp 68 3 ts-1 ts-1 NNP cord-328082-7c7slfbp 68 4 ) ) -RRB- cord-328082-7c7slfbp 68 5 was be VBD cord-328082-7c7slfbp 68 6 used use VBN cord-328082-7c7slfbp 68 7 for for IN cord-328082-7c7slfbp 68 8 all all DT cord-328082-7c7slfbp 68 9 virus virus NN cord-328082-7c7slfbp 68 10 infections infection NNS cord-328082-7c7slfbp 68 11 ( ( -LRB- cord-328082-7c7slfbp 68 12 Keranen Keranen NNP cord-328082-7c7slfbp 68 13 and and CC cord-328082-7c7slfbp 68 14 K K NNP cord-328082-7c7slfbp 68 15 ~ ~ NNP cord-328082-7c7slfbp 68 16 rifiinen rifiinen NNP cord-328082-7c7slfbp 68 17 , , , cord-328082-7c7slfbp 68 18 1975 1975 CD cord-328082-7c7slfbp 68 19 ; ; : cord-328082-7c7slfbp 68 20 Saraste Saraste NNP cord-328082-7c7slfbp 68 21 et et NNP cord-328082-7c7slfbp 68 22 al al NNP cord-328082-7c7slfbp 68 23 . . NNP cord-328082-7c7slfbp 68 24 , , , cord-328082-7c7slfbp 68 25 1980 1980 CD cord-328082-7c7slfbp 68 26 ) ) -RRB- cord-328082-7c7slfbp 68 27 . . . cord-328082-7c7slfbp 69 1 Before before IN cord-328082-7c7slfbp 69 2 infections infection NNS cord-328082-7c7slfbp 69 3 , , , cord-328082-7c7slfbp 69 4 the the DT cord-328082-7c7slfbp 69 5 cells cell NNS cord-328082-7c7slfbp 69 6 were be VBD cord-328082-7c7slfbp 69 7 washed wash VBN cord-328082-7c7slfbp 69 8 once once RB cord-328082-7c7slfbp 69 9 with with IN cord-328082-7c7slfbp 69 10 PBS PBS NNP cord-328082-7c7slfbp 69 11 and and CC cord-328082-7c7slfbp 69 12 twice twice RB cord-328082-7c7slfbp 69 13 with with IN cord-328082-7c7slfbp 69 14 serum serum NN cord-328082-7c7slfbp 69 15 - - HYPH cord-328082-7c7slfbp 69 16 free free JJ cord-328082-7c7slfbp 69 17 MEM mem NN cord-328082-7c7slfbp 69 18 ( ( -LRB- cord-328082-7c7slfbp 69 19 Gibeo Gibeo NNP cord-328082-7c7slfbp 69 20 BRL BRL NNP cord-328082-7c7slfbp 69 21 ) ) -RRB- cord-328082-7c7slfbp 69 22 . . . cord-328082-7c7slfbp 70 1 Infections infection NNS cord-328082-7c7slfbp 70 2 were be VBD cord-328082-7c7slfbp 70 3 carried carry VBN cord-328082-7c7slfbp 70 4 out out RP cord-328082-7c7slfbp 70 5 for for IN cord-328082-7c7slfbp 70 6 1 1 CD cord-328082-7c7slfbp 70 7 h h NN cord-328082-7c7slfbp 70 8 at at IN cord-328082-7c7slfbp 70 9 37~ 37~ CD cord-328082-7c7slfbp 70 10 before before IN cord-328082-7c7slfbp 70 11 the the DT cord-328082-7c7slfbp 70 12 cells cell NNS cord-328082-7c7slfbp 70 13 were be VBD cord-328082-7c7slfbp 70 14 shifted shift VBN cord-328082-7c7slfbp 70 15 to to IN cord-328082-7c7slfbp 70 16 the the DT cord-328082-7c7slfbp 70 17 restrictive restrictive JJ cord-328082-7c7slfbp 70 18 temperature temperature NN cord-328082-7c7slfbp 70 19 38.5~ 38.5~ CD cord-328082-7c7slfbp 70 20 . . . cord-328082-7c7slfbp 71 1 Depending depend VBG cord-328082-7c7slfbp 71 2 on on IN cord-328082-7c7slfbp 71 3 the the DT cord-328082-7c7slfbp 71 4 experiment experiment NN cord-328082-7c7slfbp 71 5 , , , cord-328082-7c7slfbp 71 6 the the DT cord-328082-7c7slfbp 71 7 cells cell NNS cord-328082-7c7slfbp 71 8 were be VBD cord-328082-7c7slfbp 71 9 then then RB cord-328082-7c7slfbp 71 10 shifted shift VBN cord-328082-7c7slfbp 71 11 to to IN cord-328082-7c7slfbp 71 12 the the DT cord-328082-7c7slfbp 71 13 appropriate appropriate JJ cord-328082-7c7slfbp 71 14 chase chase NN cord-328082-7c7slfbp 71 15 temperature temperature NN cord-328082-7c7slfbp 71 16 as as IN cord-328082-7c7slfbp 71 17 indicated indicate VBN cord-328082-7c7slfbp 71 18 in in IN cord-328082-7c7slfbp 71 19 the the DT cord-328082-7c7slfbp 71 20 text text NN cord-328082-7c7slfbp 71 21 . . . cord-328082-7c7slfbp 72 1 For for IN cord-328082-7c7slfbp 72 2 [ [ -LRB- cord-328082-7c7slfbp 72 3 35S]methionine 35s]methionine CD cord-328082-7c7slfbp 72 4 pulse pulse NN cord-328082-7c7slfbp 72 5 - - HYPH cord-328082-7c7slfbp 72 6 labeling label VBG cord-328082-7c7slfbp 72 7 experiments experiment NNS cord-328082-7c7slfbp 72 8 , , , cord-328082-7c7slfbp 72 9 the the DT cord-328082-7c7slfbp 72 10 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 72 11 cells cell NNS cord-328082-7c7slfbp 72 12 were be VBD cord-328082-7c7slfbp 72 13 infected infect VBN cord-328082-7c7slfbp 72 14 as as IN cord-328082-7c7slfbp 72 15 described describe VBN cord-328082-7c7slfbp 72 16 above above RB cord-328082-7c7slfbp 72 17 . . . cord-328082-7c7slfbp 73 1 Before before IN cord-328082-7c7slfbp 73 2 labeling labeling NN cord-328082-7c7slfbp 73 3 , , , cord-328082-7c7slfbp 73 4 the the DT cord-328082-7c7slfbp 73 5 cells cell NNS cord-328082-7c7slfbp 73 6 were be VBD cord-328082-7c7slfbp 73 7 grown grow VBN cord-328082-7c7slfbp 73 8 for for IN cord-328082-7c7slfbp 73 9 30 30 CD cord-328082-7c7slfbp 73 10 min min NN cord-328082-7c7slfbp 73 11 in in IN cord-328082-7c7slfbp 73 12 a a DT cord-328082-7c7slfbp 73 13 methionine methionine NN cord-328082-7c7slfbp 73 14 - - HYPH cord-328082-7c7slfbp 73 15 free free JJ cord-328082-7c7slfbp 73 16 MEM mem NN cord-328082-7c7slfbp 73 17 ( ( -LRB- cord-328082-7c7slfbp 73 18 Gibco Gibco NNP cord-328082-7c7slfbp 73 19 BRL BRL NNP cord-328082-7c7slfbp 73 20 ) ) -RRB- cord-328082-7c7slfbp 73 21 whereafter whereafter RBR cord-328082-7c7slfbp 73 22 they -PRON- PRP cord-328082-7c7slfbp 73 23 were be VBD cord-328082-7c7slfbp 73 24 pulsed pulse VBN cord-328082-7c7slfbp 73 25 for for IN cord-328082-7c7slfbp 73 26 10 10 CD cord-328082-7c7slfbp 73 27 rain rain NN cord-328082-7c7slfbp 73 28 with with IN cord-328082-7c7slfbp 73 29 200 200 CD cord-328082-7c7slfbp 73 30 # # NN cord-328082-7c7slfbp 73 31 Ci ci NN cord-328082-7c7slfbp 73 32 of of IN cord-328082-7c7slfbp 73 33 [ [ -LRB- cord-328082-7c7slfbp 73 34 32S]methionine 32s]methionine NN cord-328082-7c7slfbp 73 35 / / SYM cord-328082-7c7slfbp 73 36 dish dish NN cord-328082-7c7slfbp 73 37 . . . cord-328082-7c7slfbp 74 1 After after IN cord-328082-7c7slfbp 74 2 the the DT cord-328082-7c7slfbp 74 3 appropriate appropriate JJ cord-328082-7c7slfbp 74 4 chase chase NN cord-328082-7c7slfbp 74 5 times time NNS cord-328082-7c7slfbp 74 6 , , , cord-328082-7c7slfbp 74 7 the the DT cord-328082-7c7slfbp 74 8 cells cell NNS cord-328082-7c7slfbp 74 9 were be VBD cord-328082-7c7slfbp 74 10 washed wash VBN cord-328082-7c7slfbp 74 11 once once RB cord-328082-7c7slfbp 74 12 with with IN cord-328082-7c7slfbp 74 13 ice ice NN cord-328082-7c7slfbp 74 14 - - HYPH cord-328082-7c7slfbp 74 15 cold cold JJ cord-328082-7c7slfbp 74 16 PBS PBS NNP cord-328082-7c7slfbp 74 17 followed follow VBN cord-328082-7c7slfbp 74 18 by by IN cord-328082-7c7slfbp 74 19 solubilization solubilization NN cord-328082-7c7slfbp 74 20 for for IN cord-328082-7c7slfbp 74 21 15 15 CD cord-328082-7c7slfbp 74 22 rain rain NN cord-328082-7c7slfbp 74 23 on on IN cord-328082-7c7slfbp 74 24 ice ice NN cord-328082-7c7slfbp 74 25 with with IN cord-328082-7c7slfbp 74 26 NET net JJ cord-328082-7c7slfbp 74 27 buffer buffer NN cord-328082-7c7slfbp 74 28 ( ( -LRB- cord-328082-7c7slfbp 74 29 20 20 CD cord-328082-7c7slfbp 74 30 mM mM NNP cord-328082-7c7slfbp 74 31 Tris Tris NNP cord-328082-7c7slfbp 74 32 , , , cord-328082-7c7slfbp 74 33 pH pH NNP cord-328082-7c7slfbp 74 34 8.8 8.8 CD cord-328082-7c7slfbp 74 35 , , , cord-328082-7c7slfbp 74 36 1 1 CD cord-328082-7c7slfbp 74 37 mM mm CD cord-328082-7c7slfbp 74 38 EDTA EDTA NNP cord-328082-7c7slfbp 74 39 , , , cord-328082-7c7slfbp 74 40 1 1 CD cord-328082-7c7slfbp 74 41 % % NN cord-328082-7c7slfbp 74 42 Triton Triton NNP cord-328082-7c7slfbp 74 43 X-100 x-100 NN cord-328082-7c7slfbp 74 44 , , , cord-328082-7c7slfbp 74 45 0.4 0.4 CD cord-328082-7c7slfbp 74 46 M M NNP cord-328082-7c7slfbp 74 47 NaC1 NaC1 NNP cord-328082-7c7slfbp 74 48 , , , cord-328082-7c7slfbp 74 49 and and CC cord-328082-7c7slfbp 74 50 1 1 CD cord-328082-7c7slfbp 74 51 mM mm CD cord-328082-7c7slfbp 74 52 PMSF PMSF NNP cord-328082-7c7slfbp 74 53 ) ) -RRB- cord-328082-7c7slfbp 74 54 . . . cord-328082-7c7slfbp 75 1 Immunoprecipitations immunoprecipitation NNS cord-328082-7c7slfbp 75 2 and and CC cord-328082-7c7slfbp 75 3 endoglycosidase endoglycosidase NN cord-328082-7c7slfbp 75 4 H h NN cord-328082-7c7slfbp 75 5 ( ( -LRB- cord-328082-7c7slfbp 75 6 Endo Endo NNP cord-328082-7c7slfbp 75 7 H H NNP cord-328082-7c7slfbp 75 8 ) ) -RRB- cord-328082-7c7slfbp 75 9 analysis analysis NN cord-328082-7c7slfbp 75 10 were be VBD cord-328082-7c7slfbp 75 11 carried carry VBN cord-328082-7c7slfbp 75 12 out out RP cord-328082-7c7slfbp 75 13 as as IN cord-328082-7c7slfbp 75 14 described describe VBN cord-328082-7c7slfbp 75 15 earlier early RBR cord-328082-7c7slfbp 75 16 . . . cord-328082-7c7slfbp 76 1 The the DT cord-328082-7c7slfbp 76 2 samples sample NNS cord-328082-7c7slfbp 76 3 were be VBD cord-328082-7c7slfbp 76 4 analyzed analyze VBN cord-328082-7c7slfbp 76 5 under under IN cord-328082-7c7slfbp 76 6 reducing reduce VBG cord-328082-7c7slfbp 76 7 conditions condition NNS cord-328082-7c7slfbp 76 8 in in IN cord-328082-7c7slfbp 76 9 a a DT cord-328082-7c7slfbp 76 10 10 10 CD cord-328082-7c7slfbp 76 11 % % NN cord-328082-7c7slfbp 76 12 SDS sds NN cord-328082-7c7slfbp 76 13 - - HYPH cord-328082-7c7slfbp 76 14 PAGE PAGE NNP cord-328082-7c7slfbp 76 15 according accord VBG cord-328082-7c7slfbp 76 16 to to IN cord-328082-7c7slfbp 76 17 Laemmli Laemmli NNP cord-328082-7c7slfbp 76 18 ( ( -LRB- cord-328082-7c7slfbp 76 19 1970 1970 CD cord-328082-7c7slfbp 76 20 ) ) -RRB- cord-328082-7c7slfbp 76 21 . . . cord-328082-7c7slfbp 77 1 The the DT cord-328082-7c7slfbp 77 2 gels gel NNS cord-328082-7c7slfbp 77 3 were be VBD cord-328082-7c7slfbp 77 4 prepared prepare VBN cord-328082-7c7slfbp 77 5 for for IN cord-328082-7c7slfbp 77 6 fluorography fluorography NN cord-328082-7c7slfbp 77 7 using use VBG cord-328082-7c7slfbp 77 8 Amplify amplify VB cord-328082-7c7slfbp 77 9 TM TM NNP cord-328082-7c7slfbp 77 10 ( ( -LRB- cord-328082-7c7slfbp 77 11 Amersham Amersham NNP cord-328082-7c7slfbp 77 12 , , , cord-328082-7c7slfbp 77 13 Aylesbury Aylesbury NNP cord-328082-7c7slfbp 77 14 , , , cord-328082-7c7slfbp 77 15 UK UK NNP cord-328082-7c7slfbp 77 16 ) ) -RRB- cord-328082-7c7slfbp 77 17 . . . cord-328082-7c7slfbp 78 1 The the DT cord-328082-7c7slfbp 78 2 film film NN cord-328082-7c7slfbp 78 3 used use VBD cord-328082-7c7slfbp 78 4 for for IN cord-328082-7c7slfbp 78 5 autoradiography autoradiography NNP cord-328082-7c7slfbp 78 6 was be VBD cord-328082-7c7slfbp 78 7 Fuji Fuji NNP cord-328082-7c7slfbp 78 8 RX RX NNP cord-328082-7c7slfbp 78 9 100 100 CD cord-328082-7c7slfbp 79 1 ( ( -LRB- cord-328082-7c7slfbp 79 2 Fuji Fuji NNP cord-328082-7c7slfbp 79 3 Photo Photo NNP cord-328082-7c7slfbp 79 4 Co. Co. NNP cord-328082-7c7slfbp 79 5 Ltd. Ltd. NNP cord-328082-7c7slfbp 79 6 , , , cord-328082-7c7slfbp 79 7 Tokyo Tokyo NNP cord-328082-7c7slfbp 79 8 , , , cord-328082-7c7slfbp 79 9 Japan Japan NNP cord-328082-7c7slfbp 79 10 ) ) -RRB- cord-328082-7c7slfbp 79 11 . . . cord-328082-7c7slfbp 80 1 At at IN cord-328082-7c7slfbp 80 2 the the DT cord-328082-7c7slfbp 80 3 end end NN cord-328082-7c7slfbp 80 4 of of IN cord-328082-7c7slfbp 80 5 the the DT cord-328082-7c7slfbp 80 6 incubations incubation NNS cord-328082-7c7slfbp 80 7 , , , cord-328082-7c7slfbp 80 8 the the DT cord-328082-7c7slfbp 80 9 cells cell NNS cord-328082-7c7slfbp 80 10 were be VBD cord-328082-7c7slfbp 80 11 fixed fix VBN cord-328082-7c7slfbp 80 12 for for IN cord-328082-7c7slfbp 80 13 30 30 CD cord-328082-7c7slfbp 80 14 rain rain NN cord-328082-7c7slfbp 80 15 at at IN cord-328082-7c7slfbp 80 16 room room NN cord-328082-7c7slfbp 80 17 temperature temperature NN cord-328082-7c7slfbp 80 18 with with IN cord-328082-7c7slfbp 80 19 3 3 CD cord-328082-7c7slfbp 80 20 % % NN cord-328082-7c7slfbp 80 21 paraformaldehyde paraformaldehyde NN cord-328082-7c7slfbp 80 22 in in IN cord-328082-7c7slfbp 80 23 0.1 0.1 CD cord-328082-7c7slfbp 80 24 M m NN cord-328082-7c7slfbp 80 25 phosphate phosphate NN cord-328082-7c7slfbp 80 26 buffer buffer NN cord-328082-7c7slfbp 80 27 , , , cord-328082-7c7slfbp 80 28 pH pH NNP cord-328082-7c7slfbp 80 29 7.4 7.4 CD cord-328082-7c7slfbp 80 30 , , , cord-328082-7c7slfbp 80 31 followed follow VBN cord-328082-7c7slfbp 80 32 by by IN cord-328082-7c7slfbp 80 33 three three CD cord-328082-7c7slfbp 80 34 washes wash NNS cord-328082-7c7slfbp 80 35 with with IN cord-328082-7c7slfbp 80 36 PBS PBS NNP cord-328082-7c7slfbp 80 37 . . . cord-328082-7c7slfbp 81 1 The the DT cord-328082-7c7slfbp 81 2 cells cell NNS cord-328082-7c7slfbp 81 3 were be VBD cord-328082-7c7slfbp 81 4 then then RB cord-328082-7c7slfbp 81 5 permeabilized permeabilize VBN cord-328082-7c7slfbp 81 6 for for IN cord-328082-7c7slfbp 81 7 30 30 CD cord-328082-7c7slfbp 81 8 min min NN cord-328082-7c7slfbp 81 9 with with IN cord-328082-7c7slfbp 81 10 0.05 0.05 CD cord-328082-7c7slfbp 81 11 % % NN cord-328082-7c7slfbp 81 12 Triton Triton NNP cord-328082-7c7slfbp 81 13 X-100 x-100 NN cord-328082-7c7slfbp 81 14 in in IN cord-328082-7c7slfbp 81 15 PBS PBS NNP cord-328082-7c7slfbp 81 16 at at IN cord-328082-7c7slfbp 81 17 room room NN cord-328082-7c7slfbp 81 18 temperature temperature NN cord-328082-7c7slfbp 81 19 followed follow VBN cord-328082-7c7slfbp 81 20 by by IN cord-328082-7c7slfbp 81 21 three three CD cord-328082-7c7slfbp 81 22 washes wash NNS cord-328082-7c7slfbp 81 23 with with IN cord-328082-7c7slfbp 81 24 PBS PBS NNP cord-328082-7c7slfbp 81 25 containing contain VBG cord-328082-7c7slfbp 81 26 0.1 0.1 CD cord-328082-7c7slfbp 81 27 % % NN cord-328082-7c7slfbp 81 28 BSA BSA NNP cord-328082-7c7slfbp 81 29 . . . cord-328082-7c7slfbp 82 1 The the DT cord-328082-7c7slfbp 82 2 primary primary JJ cord-328082-7c7slfbp 82 3 antibodies antibody NNS cord-328082-7c7slfbp 82 4 were be VBD cord-328082-7c7slfbp 82 5 always always RB cord-328082-7c7slfbp 82 6 incubated incubate VBN cord-328082-7c7slfbp 82 7 for for IN cord-328082-7c7slfbp 82 8 30 30 CD cord-328082-7c7slfbp 82 9 rain rain NN cord-328082-7c7slfbp 82 10 at at IN cord-328082-7c7slfbp 82 11 37~ 37~ CD cord-328082-7c7slfbp 82 12 whereafter whereafter RBR cord-328082-7c7slfbp 82 13 the the DT cord-328082-7c7slfbp 82 14 cells cell NNS cord-328082-7c7slfbp 82 15 were be VBD cord-328082-7c7slfbp 82 16 washed wash VBN cord-328082-7c7slfbp 82 17 with with IN cord-328082-7c7slfbp 82 18 PBS PBS NNP cord-328082-7c7slfbp 82 19 containing contain VBG cord-328082-7c7slfbp 82 20 0.1 0.1 CD cord-328082-7c7slfbp 82 21 % % NN cord-328082-7c7slfbp 82 22 BSA BSA NNP cord-328082-7c7slfbp 82 23 . . . cord-328082-7c7slfbp 83 1 Incubation incubation NN cord-328082-7c7slfbp 83 2 conditions condition NNS cord-328082-7c7slfbp 83 3 for for IN cord-328082-7c7slfbp 83 4 the the DT cord-328082-7c7slfbp 83 5 second second JJ cord-328082-7c7slfbp 83 6 antibodies antibody NNS cord-328082-7c7slfbp 83 7 were be VBD cord-328082-7c7slfbp 83 8 the the DT cord-328082-7c7slfbp 83 9 same same JJ cord-328082-7c7slfbp 83 10 as as IN cord-328082-7c7slfbp 83 11 for for IN cord-328082-7c7slfbp 83 12 the the DT cord-328082-7c7slfbp 83 13 primary primary JJ cord-328082-7c7slfbp 83 14 antibodies antibody NNS cord-328082-7c7slfbp 83 15 . . . cord-328082-7c7slfbp 84 1 Finally finally RB cord-328082-7c7slfbp 84 2 , , , cord-328082-7c7slfbp 84 3 the the DT cord-328082-7c7slfbp 84 4 cells cell NNS cord-328082-7c7slfbp 84 5 were be VBD cord-328082-7c7slfbp 84 6 washed wash VBN cord-328082-7c7slfbp 84 7 and and CC cord-328082-7c7slfbp 84 8 mounted mount VBN cord-328082-7c7slfbp 84 9 in in IN cord-328082-7c7slfbp 84 10 75 75 CD cord-328082-7c7slfbp 84 11 % % NN cord-328082-7c7slfbp 84 12 glycerol glycerol NN cord-328082-7c7slfbp 84 13 , , , cord-328082-7c7slfbp 84 14 pH pH NNP cord-328082-7c7slfbp 84 15 8.5 8.5 CD cord-328082-7c7slfbp 84 16 , , , cord-328082-7c7slfbp 84 17 on on IN cord-328082-7c7slfbp 84 18 microscope microscope NN cord-328082-7c7slfbp 84 19 objective objective JJ cord-328082-7c7slfbp 84 20 glasses glass NNS cord-328082-7c7slfbp 84 21 and and CC cord-328082-7c7slfbp 84 22 viewed view VBN cord-328082-7c7slfbp 84 23 with with IN cord-328082-7c7slfbp 84 24 Zeiss Zeiss NNP cord-328082-7c7slfbp 84 25 Lab Lab NNP cord-328082-7c7slfbp 84 26 16 16 CD cord-328082-7c7slfbp 84 27 fluorescence fluorescence NN cord-328082-7c7slfbp 84 28 microscope microscope NN cord-328082-7c7slfbp 84 29 with with IN cord-328082-7c7slfbp 84 30 100x 100x JJR cord-328082-7c7slfbp 84 31 oil oil NN cord-328082-7c7slfbp 84 32 immersion immersion NN cord-328082-7c7slfbp 84 33 objective objective NN cord-328082-7c7slfbp 84 34 . . . cord-328082-7c7slfbp 85 1 Film film NN cord-328082-7c7slfbp 85 2 used use VBN cord-328082-7c7slfbp 85 3 for for IN cord-328082-7c7slfbp 85 4 photography photography NN cord-328082-7c7slfbp 85 5 was be VBD cord-328082-7c7slfbp 85 6 TMAX TMAX NNP cord-328082-7c7slfbp 85 7 400 400 CD cord-328082-7c7slfbp 85 8 ASA ASA NNP cord-328082-7c7slfbp 86 1 ( ( -LRB- cord-328082-7c7slfbp 86 2 Eastman Eastman NNP cord-328082-7c7slfbp 86 3 Kodak Kodak NNP cord-328082-7c7slfbp 86 4 Co. Co. NNP cord-328082-7c7slfbp 86 5 , , , cord-328082-7c7slfbp 86 6 Rochester Rochester NNP cord-328082-7c7slfbp 86 7 , , , cord-328082-7c7slfbp 86 8 NY NY NNP cord-328082-7c7slfbp 86 9 ) ) -RRB- cord-328082-7c7slfbp 86 10 . . . cord-328082-7c7slfbp 87 1 The the DT cord-328082-7c7slfbp 87 2 quantitation quantitation NN cord-328082-7c7slfbp 87 3 of of IN cord-328082-7c7slfbp 87 4 the the DT cord-328082-7c7slfbp 87 5 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 87 6 experiments experiment NNS cord-328082-7c7slfbp 87 7 was be VBD cord-328082-7c7slfbp 87 8 performed perform VBN cord-328082-7c7slfbp 87 9 by by IN cord-328082-7c7slfbp 87 10 photographing photograph VBG cord-328082-7c7slfbp 87 11 random random JJ cord-328082-7c7slfbp 87 12 fields field NNS cord-328082-7c7slfbp 87 13 of of IN cord-328082-7c7slfbp 87 14 the the DT cord-328082-7c7slfbp 87 15 coverglasses coverglasse NNS cord-328082-7c7slfbp 87 16 , , , cord-328082-7c7slfbp 87 17 counting count VBG cord-328082-7c7slfbp 87 18 the the DT cord-328082-7c7slfbp 87 19 cells cell NNS cord-328082-7c7slfbp 87 20 in in IN cord-328082-7c7slfbp 87 21 the the DT cord-328082-7c7slfbp 87 22 photographs photograph NNS cord-328082-7c7slfbp 87 23 , , , cord-328082-7c7slfbp 87 24 and and CC cord-328082-7c7slfbp 87 25 by by IN cord-328082-7c7slfbp 87 26 observing observe VBG cord-328082-7c7slfbp 87 27 the the DT cord-328082-7c7slfbp 87 28 distribution distribution NN cord-328082-7c7slfbp 87 29 of of IN cord-328082-7c7slfbp 87 30 man man NN cord-328082-7c7slfbp 87 31 II II NNP cord-328082-7c7slfbp 87 32 in in IN cord-328082-7c7slfbp 87 33 the the DT cord-328082-7c7slfbp 87 34 counted count VBN cord-328082-7c7slfbp 87 35 cells cell NNS cord-328082-7c7slfbp 87 36 . . . cord-328082-7c7slfbp 88 1 For for IN cord-328082-7c7slfbp 88 2 every every DT cord-328082-7c7slfbp 88 3 time time NN cord-328082-7c7slfbp 88 4 point point NN cord-328082-7c7slfbp 88 5 at at RB cord-328082-7c7slfbp 88 6 least least RBS cord-328082-7c7slfbp 88 7 150 150 CD cord-328082-7c7slfbp 88 8 cells cell NNS cord-328082-7c7slfbp 88 9 were be VBD cord-328082-7c7slfbp 88 10 counted count VBN cord-328082-7c7slfbp 88 11 . . . cord-328082-7c7slfbp 89 1 To to TO cord-328082-7c7slfbp 89 2 study study VB cord-328082-7c7slfbp 89 3 the the DT cord-328082-7c7slfbp 89 4 intracellular intracellular JJ cord-328082-7c7slfbp 89 5 distribution distribution NN cord-328082-7c7slfbp 89 6 of of IN cord-328082-7c7slfbp 89 7 p58 p58 NN cord-328082-7c7slfbp 89 8 , , , cord-328082-7c7slfbp 89 9 the the DT cord-328082-7c7slfbp 89 10 cells cell NNS cord-328082-7c7slfbp 89 11 were be VBD cord-328082-7c7slfbp 89 12 fixed fix VBN cord-328082-7c7slfbp 89 13 with with IN cord-328082-7c7slfbp 89 14 a a DT cord-328082-7c7slfbp 89 15 paraformaldehyde paraformaldehyde JJ cord-328082-7c7slfbp 89 16 - - HYPH cord-328082-7c7slfbp 89 17 lysine lysine JJ cord-328082-7c7slfbp 89 18 - - HYPH cord-328082-7c7slfbp 89 19 periodate periodate JJ cord-328082-7c7slfbp 89 20 fixative fixative NN cord-328082-7c7slfbp 89 21 ( ( -LRB- cord-328082-7c7slfbp 89 22 McLean McLean NNP cord-328082-7c7slfbp 89 23 and and CC cord-328082-7c7slfbp 89 24 Nakane Nakane NNP cord-328082-7c7slfbp 89 25 , , , cord-328082-7c7slfbp 89 26 1974 1974 CD cord-328082-7c7slfbp 89 27 ) ) -RRB- cord-328082-7c7slfbp 89 28 . . . cord-328082-7c7slfbp 90 1 The the DT cord-328082-7c7slfbp 90 2 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 90 3 microscopy microscopy NN cord-328082-7c7slfbp 90 4 procedure procedure NN cord-328082-7c7slfbp 90 5 followed follow VBN cord-328082-7c7slfbp 90 6 was be VBD cord-328082-7c7slfbp 90 7 essentially essentially RB cord-328082-7c7slfbp 90 8 that that WDT cord-328082-7c7slfbp 90 9 described describe VBN cord-328082-7c7slfbp 90 10 by by IN cord-328082-7c7slfbp 90 11 Brown Brown NNP cord-328082-7c7slfbp 90 12 and and CC cord-328082-7c7slfbp 90 13 Farquhar Farquhar NNP cord-328082-7c7slfbp 90 14 ( ( -LRB- cord-328082-7c7slfbp 90 15 1989 1989 CD cord-328082-7c7slfbp 90 16 ) ) -RRB- cord-328082-7c7slfbp 90 17 . . . cord-328082-7c7slfbp 91 1 In in IN cord-328082-7c7slfbp 91 2 conventional conventional JJ cord-328082-7c7slfbp 91 3 electron electron NN cord-328082-7c7slfbp 91 4 microscopy microscopy NN cord-328082-7c7slfbp 91 5 the the DT cord-328082-7c7slfbp 91 6 cells cell NNS cord-328082-7c7slfbp 91 7 were be VBD cord-328082-7c7slfbp 91 8 fixed fix VBN cord-328082-7c7slfbp 91 9 for for IN cord-328082-7c7slfbp 91 10 1 1 CD cord-328082-7c7slfbp 91 11 h h NN cord-328082-7c7slfbp 91 12 at at IN cord-328082-7c7slfbp 91 13 room room NN cord-328082-7c7slfbp 91 14 temperature temperature NN cord-328082-7c7slfbp 91 15 with with IN cord-328082-7c7slfbp 91 16 a a DT cord-328082-7c7slfbp 91 17 fixative fixative NN cord-328082-7c7slfbp 91 18 containing contain VBG cord-328082-7c7slfbp 91 19 2 2 CD cord-328082-7c7slfbp 91 20 % % NN cord-328082-7c7slfbp 91 21 glutaraldehyde glutaraldehyde NN cord-328082-7c7slfbp 91 22 and and CC cord-328082-7c7slfbp 91 23 3 3 CD cord-328082-7c7slfbp 91 24 % % NN cord-328082-7c7slfbp 91 25 paraformaldehyde paraformaldehyde NNP cord-328082-7c7slfbp 91 26 in in IN cord-328082-7c7slfbp 91 27 100 100 CD cord-328082-7c7slfbp 91 28 mM mm CD cord-328082-7c7slfbp 91 29 cacodylate cacodylate NN cord-328082-7c7slfbp 91 30 buffer buffer NN cord-328082-7c7slfbp 91 31 , , , cord-328082-7c7slfbp 91 32 pH pH NNP cord-328082-7c7slfbp 91 33 7.4 7.4 CD cord-328082-7c7slfbp 91 34 , , , cord-328082-7c7slfbp 91 35 and and CC cord-328082-7c7slfbp 91 36 postfixed postfixe VBD cord-328082-7c7slfbp 91 37 with with IN cord-328082-7c7slfbp 91 38 reduced reduce VBN cord-328082-7c7slfbp 91 39 1 1 CD cord-328082-7c7slfbp 91 40 % % NN cord-328082-7c7slfbp 91 41 OsO4 oso4 IN cord-328082-7c7slfbp 91 42 for for IN cord-328082-7c7slfbp 91 43 1 1 CD cord-328082-7c7slfbp 91 44 h h NN cord-328082-7c7slfbp 91 45 on on IN cord-328082-7c7slfbp 91 46 ice ice NN cord-328082-7c7slfbp 91 47 . . . cord-328082-7c7slfbp 92 1 All all DT cord-328082-7c7slfbp 92 2 samples sample NNS cord-328082-7c7slfbp 92 3 were be VBD cord-328082-7c7slfbp 92 4 embedded embed VBN cord-328082-7c7slfbp 92 5 in in IN cord-328082-7c7slfbp 93 1 LX-112 LX-112 NNP cord-328082-7c7slfbp 93 2 ( ( -LRB- cord-328082-7c7slfbp 93 3 Ladd Ladd NNP cord-328082-7c7slfbp 93 4 Research Research NNP cord-328082-7c7slfbp 93 5 Industries Industries NNPS cord-328082-7c7slfbp 93 6 Inc. Inc. NNP cord-328082-7c7slfbp 93 7 , , , cord-328082-7c7slfbp 93 8 Burlington Burlington NNP cord-328082-7c7slfbp 93 9 , , , cord-328082-7c7slfbp 93 10 VT VT NNP cord-328082-7c7slfbp 93 11 ) ) -RRB- cord-328082-7c7slfbp 93 12 and and CC cord-328082-7c7slfbp 93 13 were be VBD cord-328082-7c7slfbp 93 14 viewed view VBN cord-328082-7c7slfbp 93 15 with with IN cord-328082-7c7slfbp 93 16 a a DT cord-328082-7c7slfbp 93 17 Jeol Jeol NNP cord-328082-7c7slfbp 93 18 JEM-1200EX jem-1200ex NN cord-328082-7c7slfbp 93 19 transmission transmission NN cord-328082-7c7slfbp 93 20 electron electron NN cord-328082-7c7slfbp 93 21 microscope microscope NN cord-328082-7c7slfbp 93 22 operated operate VBN cord-328082-7c7slfbp 93 23 at at IN cord-328082-7c7slfbp 93 24 an an DT cord-328082-7c7slfbp 93 25 acceleration acceleration NN cord-328082-7c7slfbp 93 26 voltage voltage NN cord-328082-7c7slfbp 93 27 of of IN cord-328082-7c7slfbp 93 28 60 60 CD cord-328082-7c7slfbp 93 29 kV. kV. NNS cord-328082-7c7slfbp 93 30 We -PRON- PRP cord-328082-7c7slfbp 93 31 have have VBP cord-328082-7c7slfbp 93 32 previously previously RB cord-328082-7c7slfbp 93 33 shown show VBN cord-328082-7c7slfbp 93 34 that that IN cord-328082-7c7slfbp 93 35 at at IN cord-328082-7c7slfbp 93 36 20~ 20~ CD cord-328082-7c7slfbp 93 37 10 10 CD cord-328082-7c7slfbp 93 38 mM mm CD cord-328082-7c7slfbp 93 39 caffeine caffeine NN cord-328082-7c7slfbp 93 40 blocks block NNS cord-328082-7c7slfbp 93 41 efficiently efficiently RB cord-328082-7c7slfbp 93 42 the the DT cord-328082-7c7slfbp 93 43 movement movement NN cord-328082-7c7slfbp 93 44 of of IN cord-328082-7c7slfbp 93 45 newly newly RB cord-328082-7c7slfbp 93 46 synthesized synthesize VBN cord-328082-7c7slfbp 93 47 SFV sfv NN cord-328082-7c7slfbp 93 48 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 93 49 out out RP cord-328082-7c7slfbp 93 50 from from IN cord-328082-7c7slfbp 93 51 the the DT cord-328082-7c7slfbp 93 52 ER ER NNP cord-328082-7c7slfbp 93 53 . . . cord-328082-7c7slfbp 94 1 Normally normally RB cord-328082-7c7slfbp 94 2 , , , cord-328082-7c7slfbp 94 3 in in IN cord-328082-7c7slfbp 94 4 the the DT cord-328082-7c7slfbp 94 5 absence absence NN cord-328082-7c7slfbp 94 6 of of IN cord-328082-7c7slfbp 94 7 caffeine caffeine NN cord-328082-7c7slfbp 94 8 at at IN cord-328082-7c7slfbp 94 9 20~ 20~ CD cord-328082-7c7slfbp 94 10 protein protein NN cord-328082-7c7slfbp 94 11 transport transport NN cord-328082-7c7slfbp 94 12 occurs occur VBZ cord-328082-7c7slfbp 94 13 to to IN cord-328082-7c7slfbp 94 14 the the DT cord-328082-7c7slfbp 94 15 level level NN cord-328082-7c7slfbp 94 16 of of IN cord-328082-7c7slfbp 94 17 the the DT cord-328082-7c7slfbp 94 18 tmns tmn NNS cord-328082-7c7slfbp 94 19 - - HYPH cord-328082-7c7slfbp 94 20 Golgi Golgi NNP cord-328082-7c7slfbp 94 21 ( ( -LRB- cord-328082-7c7slfbp 94 22 Matlin Matlin NNP cord-328082-7c7slfbp 94 23 and and CC cord-328082-7c7slfbp 94 24 Simons Simons NNP cord-328082-7c7slfbp 94 25 , , , cord-328082-7c7slfbp 94 26 1983 1983 CD cord-328082-7c7slfbp 94 27 ; ; : cord-328082-7c7slfbp 94 28 Saraste Saraste NNP cord-328082-7c7slfbp 94 29 and and CC cord-328082-7c7slfbp 94 30 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 94 31 , , , cord-328082-7c7slfbp 94 32 1984 1984 CD cord-328082-7c7slfbp 94 33 ) ) -RRB- cord-328082-7c7slfbp 94 34 . . . cord-328082-7c7slfbp 95 1 The the DT cord-328082-7c7slfbp 95 2 exact exact JJ cord-328082-7c7slfbp 95 3 Figure figure NN cord-328082-7c7slfbp 95 4 1 1 CD cord-328082-7c7slfbp 95 5 . . . cord-328082-7c7slfbp 96 1 Determination determination NN cord-328082-7c7slfbp 96 2 of of IN cord-328082-7c7slfbp 96 3 the the DT cord-328082-7c7slfbp 96 4 temperature temperature NN cord-328082-7c7slfbp 96 5 threshold threshold NN cord-328082-7c7slfbp 96 6 allowing allow VBG cord-328082-7c7slfbp 96 7 SFV SFV NNP cord-328082-7c7slfbp 96 8 E1 E1 NNP cord-328082-7c7slfbp 96 9 to to TO cord-328082-7c7slfbp 96 10 reach reach VB cord-328082-7c7slfbp 96 11 the the DT cord-328082-7c7slfbp 96 12 Golgi Golgi NNP cord-328082-7c7slfbp 96 13 in in IN cord-328082-7c7slfbp 96 14 the the DT cord-328082-7c7slfbp 96 15 presence presence NN cord-328082-7c7slfbp 96 16 ( ( -LRB- cord-328082-7c7slfbp 96 17 a a LS cord-328082-7c7slfbp 96 18 ) ) -RRB- cord-328082-7c7slfbp 96 19 or or CC cord-328082-7c7slfbp 96 20 absence absence NN cord-328082-7c7slfbp 96 21 ( ( -LRB- cord-328082-7c7slfbp 96 22 b b NN cord-328082-7c7slfbp 96 23 ) ) -RRB- cord-328082-7c7slfbp 96 24 of of IN cord-328082-7c7slfbp 96 25 10 10 CD cord-328082-7c7slfbp 96 26 mM mm CD cord-328082-7c7slfbp 96 27 caffeine caffeine NN cord-328082-7c7slfbp 96 28 . . . cord-328082-7c7slfbp 97 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 98 1 cells cell NNS cord-328082-7c7slfbp 98 2 were be VBD cord-328082-7c7slfbp 98 3 infected infect VBN cord-328082-7c7slfbp 98 4 with with IN cord-328082-7c7slfbp 98 5 SFV SFV NNP cord-328082-7c7slfbp 98 6 ts-1 ts-1 NNP cord-328082-7c7slfbp 98 7 temperature temperature NN cord-328082-7c7slfbp 98 8 - - HYPH cord-328082-7c7slfbp 98 9 sensitive sensitive JJ cord-328082-7c7slfbp 98 10 mutant mutant NN cord-328082-7c7slfbp 98 11 , , , cord-328082-7c7slfbp 98 12 labeled label VBN cord-328082-7c7slfbp 98 13 with with IN cord-328082-7c7slfbp 98 14 [ [ -LRB- cord-328082-7c7slfbp 98 15 35S]methionine 35s]methionine CD cord-328082-7c7slfbp 98 16 and and CC cord-328082-7c7slfbp 98 17 chased chase VBN cord-328082-7c7slfbp 98 18 in in IN cord-328082-7c7slfbp 98 19 the the DT cord-328082-7c7slfbp 98 20 presence presence NN cord-328082-7c7slfbp 98 21 or or CC cord-328082-7c7slfbp 98 22 absence absence NN cord-328082-7c7slfbp 98 23 of of IN cord-328082-7c7slfbp 98 24 10 10 CD cord-328082-7c7slfbp 98 25 mM mm CD cord-328082-7c7slfbp 98 26 caffeine caffeine NN cord-328082-7c7slfbp 98 27 at at IN cord-328082-7c7slfbp 98 28 20 20 CD cord-328082-7c7slfbp 98 29 , , , cord-328082-7c7slfbp 98 30 21 21 CD cord-328082-7c7slfbp 98 31 , , , cord-328082-7c7slfbp 98 32 22 22 CD cord-328082-7c7slfbp 98 33 , , , cord-328082-7c7slfbp 98 34 23 23 CD cord-328082-7c7slfbp 98 35 , , , cord-328082-7c7slfbp 98 36 24 24 CD cord-328082-7c7slfbp 98 37 , , , cord-328082-7c7slfbp 98 38 25 25 CD cord-328082-7c7slfbp 98 39 , , , cord-328082-7c7slfbp 98 40 26 26 CD cord-328082-7c7slfbp 98 41 , , , cord-328082-7c7slfbp 98 42 and and CC cord-328082-7c7slfbp 98 43 28~ 28~ CD cord-328082-7c7slfbp 98 44 for for IN cord-328082-7c7slfbp 98 45 3 3 CD cord-328082-7c7slfbp 98 46 h. h. NN cord-328082-7c7slfbp 99 1 The the DT cord-328082-7c7slfbp 99 2 cells cell NNS cord-328082-7c7slfbp 99 3 were be VBD cord-328082-7c7slfbp 99 4 then then RB cord-328082-7c7slfbp 99 5 solubilized solubilize VBN cord-328082-7c7slfbp 99 6 , , , cord-328082-7c7slfbp 99 7 immunoprecipitated immunoprecipitate VBN cord-328082-7c7slfbp 99 8 with with IN cord-328082-7c7slfbp 99 9 monospecific monospecific JJ cord-328082-7c7slfbp 99 10 polyclonal polyclonal JJ cord-328082-7c7slfbp 99 11 antibodies antibody NNS cord-328082-7c7slfbp 99 12 to to IN cord-328082-7c7slfbp 99 13 SFV SFV NNP cord-328082-7c7slfbp 99 14 El El NNP cord-328082-7c7slfbp 99 15 , , , cord-328082-7c7slfbp 99 16 treated treat VBN cord-328082-7c7slfbp 99 17 with with IN cord-328082-7c7slfbp 99 18 Endo Endo NNP cord-328082-7c7slfbp 99 19 H H NNP cord-328082-7c7slfbp 99 20 , , , cord-328082-7c7slfbp 99 21 and and CC cord-328082-7c7slfbp 99 22 run run VB cord-328082-7c7slfbp 99 23 in in IN cord-328082-7c7slfbp 99 24 a a DT cord-328082-7c7slfbp 99 25 10 10 CD cord-328082-7c7slfbp 99 26 % % NN cord-328082-7c7slfbp 99 27 SDS sds NN cord-328082-7c7slfbp 99 28 - - HYPH cord-328082-7c7slfbp 99 29 PAGE page NN cord-328082-7c7slfbp 99 30 under under IN cord-328082-7c7slfbp 99 31 reducing reduce VBG cord-328082-7c7slfbp 99 32 conditions condition NNS cord-328082-7c7slfbp 99 33 . . . cord-328082-7c7slfbp 100 1 From from IN cord-328082-7c7slfbp 100 2 a a DT cord-328082-7c7slfbp 100 3 it -PRON- PRP cord-328082-7c7slfbp 100 4 can can MD cord-328082-7c7slfbp 100 5 be be VB cord-328082-7c7slfbp 100 6 observed observe VBN cord-328082-7c7slfbp 100 7 that that IN cord-328082-7c7slfbp 100 8 SFV SFV NNP cord-328082-7c7slfbp 100 9 E1 e1 NN cord-328082-7c7slfbp 100 10 remains remain VBZ cord-328082-7c7slfbp 100 11 Endo Endo NNP cord-328082-7c7slfbp 100 12 H H NNP cord-328082-7c7slfbp 100 13 sensitive sensitive JJ cord-328082-7c7slfbp 100 14 in in IN cord-328082-7c7slfbp 100 15 the the DT cord-328082-7c7slfbp 100 16 presence presence NN cord-328082-7c7slfbp 100 17 of of IN cord-328082-7c7slfbp 100 18 10 10 CD cord-328082-7c7slfbp 100 19 mM mm CD cord-328082-7c7slfbp 100 20 caffeine caffeine NN cord-328082-7c7slfbp 100 21 up up IN cord-328082-7c7slfbp 100 22 to to IN cord-328082-7c7slfbp 100 23 240C. 240C. NNP cord-328082-7c7slfbp 100 24 E1 E1 NNP cord-328082-7c7slfbp 100 25 becomes become VBZ cord-328082-7c7slfbp 100 26 gradually gradually RB cord-328082-7c7slfbp 100 27 resistant resistant JJ cord-328082-7c7slfbp 100 28 to to IN cord-328082-7c7slfbp 100 29 Endo Endo NNP cord-328082-7c7slfbp 100 30 H H NNP cord-328082-7c7slfbp 100 31 treatment treatment NN cord-328082-7c7slfbp 100 32 at at IN cord-328082-7c7slfbp 100 33 250C 250c CD cord-328082-7c7slfbp 100 34 and and CC cord-328082-7c7slfbp 100 35 at at IN cord-328082-7c7slfbp 100 36 higher high JJR cord-328082-7c7slfbp 100 37 temperatures temperature NNS cord-328082-7c7slfbp 100 38 , , , cord-328082-7c7slfbp 100 39 indicating indicate VBG cord-328082-7c7slfbp 100 40 the the DT cord-328082-7c7slfbp 100 41 arrival arrival NN cord-328082-7c7slfbp 100 42 of of IN cord-328082-7c7slfbp 100 43 E1 E1 NNP cord-328082-7c7slfbp 100 44 to to IN cord-328082-7c7slfbp 100 45 the the DT cord-328082-7c7slfbp 100 46 Golgi Golgi NNP cord-328082-7c7slfbp 100 47 stack stack NN cord-328082-7c7slfbp 100 48 . . . cord-328082-7c7slfbp 101 1 In in IN cord-328082-7c7slfbp 101 2 b b NN cord-328082-7c7slfbp 101 3 the the DT cord-328082-7c7slfbp 101 4 sensitivity sensitivity NN cord-328082-7c7slfbp 101 5 of of IN cord-328082-7c7slfbp 101 6 E1 e1 NN cord-328082-7c7slfbp 101 7 to to IN cord-328082-7c7slfbp 101 8 Endo Endo NNP cord-328082-7c7slfbp 101 9 H H NNP cord-328082-7c7slfbp 101 10 treatment treatment NN cord-328082-7c7slfbp 101 11 in in IN cord-328082-7c7slfbp 101 12 the the DT cord-328082-7c7slfbp 101 13 absence absence NN cord-328082-7c7slfbp 101 14 of of IN cord-328082-7c7slfbp 101 15 caffeine caffeine NN cord-328082-7c7slfbp 101 16 at at IN cord-328082-7c7slfbp 101 17 different different JJ cord-328082-7c7slfbp 101 18 temperatures temperature NNS cord-328082-7c7slfbp 101 19 is be VBZ cord-328082-7c7slfbp 101 20 shown show VBN cord-328082-7c7slfbp 101 21 . . . cord-328082-7c7slfbp 102 1 In in IN cord-328082-7c7slfbp 102 2 the the DT cord-328082-7c7slfbp 102 3 absence absence NN cord-328082-7c7slfbp 102 4 of of IN cord-328082-7c7slfbp 102 5 caffeine caffeine NN cord-328082-7c7slfbp 102 6 , , , cord-328082-7c7slfbp 102 7 E1 E1 NNP cord-328082-7c7slfbp 102 8 becomes become VBZ cord-328082-7c7slfbp 102 9 Endo Endo NNP cord-328082-7c7slfbp 102 10 H H NNP cord-328082-7c7slfbp 102 11 resistant resistant JJ cord-328082-7c7slfbp 102 12 already already RB cord-328082-7c7slfbp 102 13 at at IN cord-328082-7c7slfbp 102 14 20~ 20~ CD cord-328082-7c7slfbp 102 15 ( ( -LRB- cord-328082-7c7slfbp 102 16 b b NN cord-328082-7c7slfbp 102 17 ) ) -RRB- cord-328082-7c7slfbp 102 18 . . . cord-328082-7c7slfbp 103 1 P p NN cord-328082-7c7slfbp 103 2 , , , cord-328082-7c7slfbp 103 3 pulse pulse NN cord-328082-7c7slfbp 103 4 . . . cord-328082-7c7slfbp 104 1 temperature temperature NN cord-328082-7c7slfbp 104 2 threshold threshold NN cord-328082-7c7slfbp 104 3 that that WDT cord-328082-7c7slfbp 104 4 allows allow VBZ cord-328082-7c7slfbp 104 5 SFV sfv NN cord-328082-7c7slfbp 104 6 E1 e1 NN cord-328082-7c7slfbp 104 7 membrane membrane NN cord-328082-7c7slfbp 104 8 glycoprotein glycoprotein NN cord-328082-7c7slfbp 104 9 to to TO cord-328082-7c7slfbp 104 10 reach reach VB cord-328082-7c7slfbp 104 11 the the DT cord-328082-7c7slfbp 104 12 Golgi Golgi NNP cord-328082-7c7slfbp 104 13 stack stack NN cord-328082-7c7slfbp 104 14 in in IN cord-328082-7c7slfbp 104 15 the the DT cord-328082-7c7slfbp 104 16 presence presence NN cord-328082-7c7slfbp 104 17 of of IN cord-328082-7c7slfbp 104 18 caffeine caffeine NN cord-328082-7c7slfbp 104 19 was be VBD cord-328082-7c7slfbp 104 20 analyzed analyze VBN cord-328082-7c7slfbp 104 21 by by IN cord-328082-7c7slfbp 104 22 following follow VBG cord-328082-7c7slfbp 104 23 the the DT cord-328082-7c7slfbp 104 24 maturation maturation NN cord-328082-7c7slfbp 104 25 of of IN cord-328082-7c7slfbp 104 26 SFV SFV NNP cord-328082-7c7slfbp 104 27 El El NNP cord-328082-7c7slfbp 104 28 - - HYPH cord-328082-7c7slfbp 104 29 linked link VBN cord-328082-7c7slfbp 104 30 glycans glycan NNS cord-328082-7c7slfbp 104 31 to to IN cord-328082-7c7slfbp 104 32 the the DT cord-328082-7c7slfbp 104 33 Endo Endo NNP cord-328082-7c7slfbp 104 34 H H NNP cord-328082-7c7slfbp 104 35 - - HYPH cord-328082-7c7slfbp 104 36 resistant resistant JJ cord-328082-7c7slfbp 104 37 form form NN cord-328082-7c7slfbp 104 38 . . . cord-328082-7c7slfbp 105 1 We -PRON- PRP cord-328082-7c7slfbp 105 2 also also RB cord-328082-7c7slfbp 105 3 carried carry VBD cord-328082-7c7slfbp 105 4 out out RP cord-328082-7c7slfbp 105 5 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 105 6 experiments experiment NNS cord-328082-7c7slfbp 105 7 to to TO cord-328082-7c7slfbp 105 8 analyze analyze VB cord-328082-7c7slfbp 105 9 the the DT cord-328082-7c7slfbp 105 10 exact exact JJ cord-328082-7c7slfbp 105 11 temperature temperature NN cord-328082-7c7slfbp 105 12 that that WDT cord-328082-7c7slfbp 105 13 allows allow VBZ cord-328082-7c7slfbp 105 14 SFV sfv NN cord-328082-7c7slfbp 105 15 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 105 16 to to TO cord-328082-7c7slfbp 105 17 exit exit VB cord-328082-7c7slfbp 105 18 the the DT cord-328082-7c7slfbp 105 19 ER er NN cord-328082-7c7slfbp 105 20 . . . cord-328082-7c7slfbp 106 1 These these DT cord-328082-7c7slfbp 106 2 experiments experiment NNS cord-328082-7c7slfbp 106 3 together together RB cord-328082-7c7slfbp 106 4 should should MD cord-328082-7c7slfbp 106 5 further further RB cord-328082-7c7slfbp 106 6 reveal reveal VB cord-328082-7c7slfbp 106 7 whether whether IN cord-328082-7c7slfbp 106 8 caffeine caffeine NN cord-328082-7c7slfbp 106 9 at at IN cord-328082-7c7slfbp 106 10 reduced reduce VBN cord-328082-7c7slfbp 106 11 temperature temperature NN cord-328082-7c7slfbp 106 12 could could MD cord-328082-7c7slfbp 106 13 arrest arrest VB cord-328082-7c7slfbp 106 14 the the DT cord-328082-7c7slfbp 106 15 ER ER NNP cord-328082-7c7slfbp 106 16 - - HYPH cord-328082-7c7slfbp 106 17 to to IN cord-328082-7c7slfbp 106 18 - - HYPH cord-328082-7c7slfbp 106 19 Golgi golgi NN cord-328082-7c7slfbp 106 20 traffic traffic NN cord-328082-7c7slfbp 106 21 in in IN cord-328082-7c7slfbp 106 22 possible possible JJ cord-328082-7c7slfbp 106 23 intermediate intermediate JJ cord-328082-7c7slfbp 106 24 steps step NNS cord-328082-7c7slfbp 106 25 . . . cord-328082-7c7slfbp 107 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 108 1 cells cell NNS cord-328082-7c7slfbp 108 2 were be VBD cord-328082-7c7slfbp 108 3 infected infect VBN cord-328082-7c7slfbp 108 4 with with IN cord-328082-7c7slfbp 108 5 SFV SFV NNP cord-328082-7c7slfbp 108 6 ts-1 ts-1 NNP cord-328082-7c7slfbp 108 7 , , , cord-328082-7c7slfbp 108 8 shifted shift VBD cord-328082-7c7slfbp 108 9 to to IN cord-328082-7c7slfbp 108 10 the the DT cord-328082-7c7slfbp 108 11 restrictive restrictive JJ cord-328082-7c7slfbp 108 12 temperature temperature NN cord-328082-7c7slfbp 108 13 , , , cord-328082-7c7slfbp 108 14 labeled label VBN cord-328082-7c7slfbp 108 15 at at IN cord-328082-7c7slfbp 108 16 3 3 CD cord-328082-7c7slfbp 108 17 h h NN cord-328082-7c7slfbp 108 18 45 45 CD cord-328082-7c7slfbp 108 19 min min NN cord-328082-7c7slfbp 108 20 after after IN cord-328082-7c7slfbp 108 21 infection infection NN cord-328082-7c7slfbp 108 22 with with IN cord-328082-7c7slfbp 108 23 [ [ -LRB- cord-328082-7c7slfbp 108 24 35S]methionine 35s]methionine CD cord-328082-7c7slfbp 108 25 for for IN cord-328082-7c7slfbp 108 26 10 10 CD cord-328082-7c7slfbp 108 27 min min NN cord-328082-7c7slfbp 108 28 and and CC cord-328082-7c7slfbp 108 29 chased chase VBN cord-328082-7c7slfbp 108 30 for for IN cord-328082-7c7slfbp 108 31 3 3 CD cord-328082-7c7slfbp 108 32 h h NN cord-328082-7c7slfbp 108 33 at at IN cord-328082-7c7slfbp 108 34 20 20 CD cord-328082-7c7slfbp 108 35 , , , cord-328082-7c7slfbp 108 36 21 21 CD cord-328082-7c7slfbp 108 37 , , , cord-328082-7c7slfbp 108 38 22 22 CD cord-328082-7c7slfbp 108 39 , , , cord-328082-7c7slfbp 108 40 23 23 CD cord-328082-7c7slfbp 108 41 , , , cord-328082-7c7slfbp 108 42 24 24 CD cord-328082-7c7slfbp 108 43 , , , cord-328082-7c7slfbp 108 44 25 25 CD cord-328082-7c7slfbp 108 45 , , , cord-328082-7c7slfbp 108 46 26 26 CD cord-328082-7c7slfbp 108 47 , , , cord-328082-7c7slfbp 108 48 and and CC cord-328082-7c7slfbp 108 49 28~ 28~ CD cord-328082-7c7slfbp 108 50 with with IN cord-328082-7c7slfbp 108 51 or or CC cord-328082-7c7slfbp 108 52 without without IN cord-328082-7c7slfbp 108 53 10 10 CD cord-328082-7c7slfbp 108 54 mM mm CD cord-328082-7c7slfbp 108 55 caffeine caffeine NN cord-328082-7c7slfbp 108 56 . . . cord-328082-7c7slfbp 109 1 E1 e1 NN cord-328082-7c7slfbp 109 2 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 109 3 were be VBD cord-328082-7c7slfbp 109 4 then then RB cord-328082-7c7slfbp 109 5 immunoprecipitated immunoprecipitate VBN cord-328082-7c7slfbp 109 6 and and CC cord-328082-7c7slfbp 109 7 analyzed analyze VBD cord-328082-7c7slfbp 109 8 for for IN cord-328082-7c7slfbp 109 9 their -PRON- PRP$ cord-328082-7c7slfbp 109 10 sensitivity sensitivity NN cord-328082-7c7slfbp 109 11 to to IN cord-328082-7c7slfbp 109 12 Endo Endo NNP cord-328082-7c7slfbp 109 13 H. H. NNP cord-328082-7c7slfbp 110 1 In in IN cord-328082-7c7slfbp 110 2 the the DT cord-328082-7c7slfbp 110 3 presence presence NN cord-328082-7c7slfbp 110 4 of of IN cord-328082-7c7slfbp 110 5 10 10 CD cord-328082-7c7slfbp 110 6 mM mm CD cord-328082-7c7slfbp 110 7 caffeine caffeine NN cord-328082-7c7slfbp 110 8 , , , cord-328082-7c7slfbp 110 9 SFV SFV NNP cord-328082-7c7slfbp 110 10 El El NNP cord-328082-7c7slfbp 110 11 - - HYPH cord-328082-7c7slfbp 110 12 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 110 13 remained remain VBD cord-328082-7c7slfbp 110 14 Endo Endo NNP cord-328082-7c7slfbp 110 15 H H NNP cord-328082-7c7slfbp 110 16 sensitive sensitive JJ cord-328082-7c7slfbp 110 17 up up RP cord-328082-7c7slfbp 110 18 to to IN cord-328082-7c7slfbp 110 19 24~ 24~ CD cord-328082-7c7slfbp 111 1 ( ( -LRB- cord-328082-7c7slfbp 111 2 Fig Fig NNP cord-328082-7c7slfbp 111 3 . . . cord-328082-7c7slfbp 111 4 1 1 CD cord-328082-7c7slfbp 111 5 a a LS cord-328082-7c7slfbp 111 6 ) ) -RRB- cord-328082-7c7slfbp 111 7 . . . cord-328082-7c7slfbp 112 1 At at IN cord-328082-7c7slfbp 112 2 25~ 25~ CD cord-328082-7c7slfbp 112 3 and and CC cord-328082-7c7slfbp 112 4 at at IN cord-328082-7c7slfbp 112 5 higher high JJR cord-328082-7c7slfbp 112 6 temperatures temperature NNS cord-328082-7c7slfbp 112 7 E1 e1 NN cord-328082-7c7slfbp 112 8 reached reach VBD cord-328082-7c7slfbp 112 9 the the DT cord-328082-7c7slfbp 112 10 Golgi Golgi NNP cord-328082-7c7slfbp 112 11 stack stack NN cord-328082-7c7slfbp 112 12 as as IN cord-328082-7c7slfbp 112 13 indicated indicate VBN cord-328082-7c7slfbp 112 14 by by IN cord-328082-7c7slfbp 112 15 the the DT cord-328082-7c7slfbp 112 16 increasing increase VBG cord-328082-7c7slfbp 112 17 amount amount NN cord-328082-7c7slfbp 112 18 of of IN cord-328082-7c7slfbp 112 19 Endo Endo NNP cord-328082-7c7slfbp 112 20 H H NNP cord-328082-7c7slfbp 112 21 - - HYPH cord-328082-7c7slfbp 112 22 resistant resistant JJ cord-328082-7c7slfbp 112 23 form form NN cord-328082-7c7slfbp 112 24 of of IN cord-328082-7c7slfbp 112 25 E1 e1 NN cord-328082-7c7slfbp 112 26 ( ( -LRB- cord-328082-7c7slfbp 112 27 Fig Fig NNP cord-328082-7c7slfbp 112 28 . . . cord-328082-7c7slfbp 112 29 1 1 CD cord-328082-7c7slfbp 112 30 a a LS cord-328082-7c7slfbp 112 31 ) ) -RRB- cord-328082-7c7slfbp 112 32 . . . cord-328082-7c7slfbp 113 1 In in IN cord-328082-7c7slfbp 113 2 control control NN cord-328082-7c7slfbp 113 3 cells cell NNS cord-328082-7c7slfbp 113 4 ( ( -LRB- cord-328082-7c7slfbp 113 5 Fig Fig NNP cord-328082-7c7slfbp 113 6 . . . cord-328082-7c7slfbp 113 7 1 1 CD cord-328082-7c7slfbp 113 8 b b NN cord-328082-7c7slfbp 113 9 ) ) -RRB- cord-328082-7c7slfbp 113 10 , , , cord-328082-7c7slfbp 113 11 E1 E1 NNP cord-328082-7c7slfbp 113 12 reached reach VBD cord-328082-7c7slfbp 113 13 the the DT cord-328082-7c7slfbp 113 14 Golgi Golgi NNP cord-328082-7c7slfbp 113 15 stack stack NN cord-328082-7c7slfbp 113 16 already already RB cord-328082-7c7slfbp 113 17 at at IN cord-328082-7c7slfbp 113 18 20~ 20~ CD cord-328082-7c7slfbp 113 19 as as IN cord-328082-7c7slfbp 113 20 indicated indicate VBN cord-328082-7c7slfbp 113 21 by by IN cord-328082-7c7slfbp 113 22 the the DT cord-328082-7c7slfbp 113 23 maturation maturation NN cord-328082-7c7slfbp 113 24 of of IN cord-328082-7c7slfbp 113 25 the the DT cord-328082-7c7slfbp 113 26 proteins protein NNS cord-328082-7c7slfbp 113 27 to to IN cord-328082-7c7slfbp 113 28 the the DT cord-328082-7c7slfbp 113 29 Endo Endo NNP cord-328082-7c7slfbp 113 30 H H NNP cord-328082-7c7slfbp 113 31 - - HYPH cord-328082-7c7slfbp 113 32 resistant resistant JJ cord-328082-7c7slfbp 113 33 form form NN cord-328082-7c7slfbp 113 34 . . . cord-328082-7c7slfbp 114 1 In in IN cord-328082-7c7slfbp 114 2 Fig Fig NNP cord-328082-7c7slfbp 114 3 . . . cord-328082-7c7slfbp 115 1 1 1 CD cord-328082-7c7slfbp 115 2 b b NN cord-328082-7c7slfbp 115 3 , , , cord-328082-7c7slfbp 115 4 it -PRON- PRP cord-328082-7c7slfbp 115 5 can can MD cord-328082-7c7slfbp 115 6 be be VB cord-328082-7c7slfbp 115 7 seen see VBN cord-328082-7c7slfbp 115 8 that that IN cord-328082-7c7slfbp 115 9 E1 e1 NN cord-328082-7c7slfbp 115 10 does do VBZ cord-328082-7c7slfbp 115 11 not not RB cord-328082-7c7slfbp 115 12 become become VB cord-328082-7c7slfbp 115 13 completely completely RB cord-328082-7c7slfbp 115 14 resistant resistant JJ cord-328082-7c7slfbp 115 15 to to IN cord-328082-7c7slfbp 115 16 Endo Endo NNP cord-328082-7c7slfbp 115 17 H H NNP cord-328082-7c7slfbp 115 18 treatment treatment NN cord-328082-7c7slfbp 115 19 even even RB cord-328082-7c7slfbp 115 20 at at IN cord-328082-7c7slfbp 115 21 28~ 28~ CD cord-328082-7c7slfbp 116 1 This this DT cord-328082-7c7slfbp 116 2 , , , cord-328082-7c7slfbp 116 3 however however RB cord-328082-7c7slfbp 116 4 , , , cord-328082-7c7slfbp 116 5 is be VBZ cord-328082-7c7slfbp 116 6 a a DT cord-328082-7c7slfbp 116 7 constant constant JJ cord-328082-7c7slfbp 116 8 finding finding NN cord-328082-7c7slfbp 116 9 for for IN cord-328082-7c7slfbp 116 10 SFV SFV NNP cord-328082-7c7slfbp 116 11 ts-1 ts-1 NNP cord-328082-7c7slfbp 116 12 and and CC cord-328082-7c7slfbp 116 13 has have VBZ cord-328082-7c7slfbp 116 14 been be VBN cord-328082-7c7slfbp 116 15 reported report VBN cord-328082-7c7slfbp 116 16 and and CC cord-328082-7c7slfbp 116 17 discussed discuss VBN cord-328082-7c7slfbp 116 18 earlier early RBR cord-328082-7c7slfbp 116 19 ( ( -LRB- cord-328082-7c7slfbp 116 20 Saraste Saraste NNP cord-328082-7c7slfbp 116 21 and and CC cord-328082-7c7slfbp 116 22 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 116 23 , , , cord-328082-7c7slfbp 116 24 1984 1984 CD cord-328082-7c7slfbp 116 25 ; ; : cord-328082-7c7slfbp 116 26 Gahmberg Gahmberg NNP cord-328082-7c7slfbp 116 27 et et FW cord-328082-7c7slfbp 116 28 al al NNP cord-328082-7c7slfbp 116 29 . . NNP cord-328082-7c7slfbp 116 30 , , , cord-328082-7c7slfbp 116 31 1986 1986 CD cord-328082-7c7slfbp 116 32 ) ) -RRB- cord-328082-7c7slfbp 116 33 . . . cord-328082-7c7slfbp 117 1 Since since IN cord-328082-7c7slfbp 117 2 the the DT cord-328082-7c7slfbp 117 3 acquisition acquisition NN cord-328082-7c7slfbp 117 4 of of IN cord-328082-7c7slfbp 117 5 Endo Endo NNP cord-328082-7c7slfbp 117 6 H H NNP cord-328082-7c7slfbp 117 7 resistance resistance NN cord-328082-7c7slfbp 117 8 indicates indicate VBZ cord-328082-7c7slfbp 117 9 only only RB cord-328082-7c7slfbp 117 10 that that IN cord-328082-7c7slfbp 117 11 E1 E1 NNP cord-328082-7c7slfbp 117 12 has have VBZ cord-328082-7c7slfbp 117 13 reached reach VBN cord-328082-7c7slfbp 117 14 the the DT cord-328082-7c7slfbp 117 15 Golgi Golgi NNP cord-328082-7c7slfbp 117 16 stack stack NN cord-328082-7c7slfbp 117 17 , , , cord-328082-7c7slfbp 117 18 we -PRON- PRP cord-328082-7c7slfbp 117 19 next next RB cord-328082-7c7slfbp 117 20 studied study VBD cord-328082-7c7slfbp 117 21 whether whether IN cord-328082-7c7slfbp 117 22 SFV sfv NN cord-328082-7c7slfbp 117 23 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 117 24 can can MD cord-328082-7c7slfbp 117 25 exit exit VB cord-328082-7c7slfbp 117 26 the the DT cord-328082-7c7slfbp 117 27 ER er NN cord-328082-7c7slfbp 117 28 at at IN cord-328082-7c7slfbp 117 29 temperatures temperature NNS cord-328082-7c7slfbp 117 30 between between IN cord-328082-7c7slfbp 117 31 200C 200c CD cord-328082-7c7slfbp 117 32 and and CC cord-328082-7c7slfbp 117 33 25~ 25~ CD cord-328082-7c7slfbp 117 34 and and CC cord-328082-7c7slfbp 117 35 whether whether IN cord-328082-7c7slfbp 117 36 they -PRON- PRP cord-328082-7c7slfbp 117 37 are be VBP cord-328082-7c7slfbp 117 38 observed observe VBN cord-328082-7c7slfbp 117 39 to to TO cord-328082-7c7slfbp 117 40 accumulate accumulate VB cord-328082-7c7slfbp 117 41 in in IN cord-328082-7c7slfbp 117 42 intermediate intermediate JJ cord-328082-7c7slfbp 117 43 elements element NNS cord-328082-7c7slfbp 117 44 between between IN cord-328082-7c7slfbp 117 45 the the DT cord-328082-7c7slfbp 117 46 ER ER NNP cord-328082-7c7slfbp 117 47 and and CC cord-328082-7c7slfbp 117 48 the the DT cord-328082-7c7slfbp 117 49 Golgi Golgi NNP cord-328082-7c7slfbp 117 50 compartment compartment NN cord-328082-7c7slfbp 117 51 . . . cord-328082-7c7slfbp 118 1 BHK-21 bhk-21 DT cord-328082-7c7slfbp 118 2 cells cell NNS cord-328082-7c7slfbp 118 3 were be VBD cord-328082-7c7slfbp 118 4 infected infect VBN cord-328082-7c7slfbp 118 5 with with IN cord-328082-7c7slfbp 118 6 SFV SFV NNP cord-328082-7c7slfbp 118 7 ts-1 ts-1 NNP cord-328082-7c7slfbp 118 8 for for IN cord-328082-7c7slfbp 118 9 1 1 CD cord-328082-7c7slfbp 118 10 h h NN cord-328082-7c7slfbp 118 11 , , , cord-328082-7c7slfbp 118 12 shifted shift VBD cord-328082-7c7slfbp 118 13 to to IN cord-328082-7c7slfbp 118 14 restrictive restrictive JJ cord-328082-7c7slfbp 118 15 temperature temperature NN cord-328082-7c7slfbp 118 16 ( ( -LRB- cord-328082-7c7slfbp 118 17 38.5~ 38.5~ CD cord-328082-7c7slfbp 118 18 and and CC cord-328082-7c7slfbp 118 19 grown grow VBN cord-328082-7c7slfbp 118 20 there there RB cord-328082-7c7slfbp 118 21 for for IN cord-328082-7c7slfbp 118 22 4 4 CD cord-328082-7c7slfbp 118 23 h. h. NN cord-328082-7c7slfbp 119 1 The the DT cord-328082-7c7slfbp 119 2 cells cell NNS cord-328082-7c7slfbp 119 3 were be VBD cord-328082-7c7slfbp 119 4 then then RB cord-328082-7c7slfbp 119 5 shifted shift VBN cord-328082-7c7slfbp 119 6 to to IN cord-328082-7c7slfbp 119 7 20 20 CD cord-328082-7c7slfbp 119 8 , , , cord-328082-7c7slfbp 119 9 21 21 CD cord-328082-7c7slfbp 119 10 , , , cord-328082-7c7slfbp 119 11 22 22 CD cord-328082-7c7slfbp 119 12 , , , cord-328082-7c7slfbp 119 13 23 23 CD cord-328082-7c7slfbp 119 14 , , , cord-328082-7c7slfbp 119 15 24 24 CD cord-328082-7c7slfbp 119 16 , , , cord-328082-7c7slfbp 119 17 25 25 CD cord-328082-7c7slfbp 119 18 , , , cord-328082-7c7slfbp 119 19 and and CC cord-328082-7c7slfbp 119 20 280C 280C NNP cord-328082-7c7slfbp 119 21 , , , cord-328082-7c7slfbp 119 22 and and CC cord-328082-7c7slfbp 119 23 chased chase VBD cord-328082-7c7slfbp 119 24 for for IN cord-328082-7c7slfbp 119 25 2 2 CD cord-328082-7c7slfbp 119 26 h h NN cord-328082-7c7slfbp 119 27 in in IN cord-328082-7c7slfbp 119 28 the the DT cord-328082-7c7slfbp 119 29 presence presence NN cord-328082-7c7slfbp 119 30 or or CC cord-328082-7c7slfbp 119 31 absence absence NN cord-328082-7c7slfbp 119 32 of of IN cord-328082-7c7slfbp 119 33 10 10 CD cord-328082-7c7slfbp 119 34 mM mm CD cord-328082-7c7slfbp 119 35 caffeine caffeine NN cord-328082-7c7slfbp 119 36 . . . cord-328082-7c7slfbp 120 1 To to TO cord-328082-7c7slfbp 120 2 be be VB cord-328082-7c7slfbp 120 3 able able JJ cord-328082-7c7slfbp 120 4 to to TO cord-328082-7c7slfbp 120 5 follow follow VB cord-328082-7c7slfbp 120 6 the the DT cord-328082-7c7slfbp 120 7 movement movement NN cord-328082-7c7slfbp 120 8 of of IN cord-328082-7c7slfbp 120 9 presynthetized presynthetized JJ cord-328082-7c7slfbp 120 10 E1 E1 NNP cord-328082-7c7slfbp 120 11 and and CC cord-328082-7c7slfbp 120 12 E2 E2 NNP cord-328082-7c7slfbp 120 13 , , , cord-328082-7c7slfbp 120 14 the the DT cord-328082-7c7slfbp 120 15 chase chase NN cord-328082-7c7slfbp 120 16 mediums medium NNS cord-328082-7c7slfbp 120 17 contained contain VBD cord-328082-7c7slfbp 120 18 50 50 CD cord-328082-7c7slfbp 120 19 t t NN cord-328082-7c7slfbp 120 20 ~ ~ CD cord-328082-7c7slfbp 120 21 g g NN cord-328082-7c7slfbp 120 22 / / SYM cord-328082-7c7slfbp 120 23 ml ml NN cord-328082-7c7slfbp 120 24 of of IN cord-328082-7c7slfbp 120 25 cycloheximide cycloheximide NN cord-328082-7c7slfbp 120 26 . . . cord-328082-7c7slfbp 121 1 In in IN cord-328082-7c7slfbp 121 2 cells cell NNS cord-328082-7c7slfbp 121 3 incubated incubate VBN cord-328082-7c7slfbp 121 4 at at IN cord-328082-7c7slfbp 121 5 20 20 CD cord-328082-7c7slfbp 121 6 , , , cord-328082-7c7slfbp 121 7 21 21 CD cord-328082-7c7slfbp 121 8 , , , cord-328082-7c7slfbp 121 9 22 22 CD cord-328082-7c7slfbp 121 10 , , , cord-328082-7c7slfbp 121 11 and and CC cord-328082-7c7slfbp 121 12 23~ 23~ CD cord-328082-7c7slfbp 121 13 in in IN cord-328082-7c7slfbp 121 14 the the DT cord-328082-7c7slfbp 121 15 presence presence NN cord-328082-7c7slfbp 121 16 of of IN cord-328082-7c7slfbp 121 17 caffeine caffeine NN cord-328082-7c7slfbp 121 18 , , , cord-328082-7c7slfbp 121 19 the the DT cord-328082-7c7slfbp 121 20 SFV sfv NN cord-328082-7c7slfbp 121 21 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 121 22 remained remain VBD cord-328082-7c7slfbp 121 23 in in IN cord-328082-7c7slfbp 121 24 a a DT cord-328082-7c7slfbp 121 25 reticular reticular JJ cord-328082-7c7slfbp 121 26 structure structure NN cord-328082-7c7slfbp 121 27 ( ( -LRB- cord-328082-7c7slfbp 121 28 Fig Fig NNP cord-328082-7c7slfbp 121 29 . . NNP cord-328082-7c7slfbp 121 30 2 2 CD cord-328082-7c7slfbp 121 31 , , , cord-328082-7c7slfbp 121 32 b b NN cord-328082-7c7slfbp 121 33 , , , cord-328082-7c7slfbp 121 34 c c NNP cord-328082-7c7slfbp 121 35 , , , cord-328082-7c7slfbp 121 36 d d NNP cord-328082-7c7slfbp 121 37 , , , cord-328082-7c7slfbp 121 38 and and CC cord-328082-7c7slfbp 121 39 e e NNP cord-328082-7c7slfbp 121 40 , , , cord-328082-7c7slfbp 121 41 respectively respectively RB cord-328082-7c7slfbp 121 42 ) ) -RRB- cord-328082-7c7slfbp 121 43 identical identical JJ cord-328082-7c7slfbp 121 44 to to IN cord-328082-7c7slfbp 121 45 the the DT cord-328082-7c7slfbp 121 46 ER ER NNP cord-328082-7c7slfbp 121 47 labeling labeling NN cord-328082-7c7slfbp 121 48 observed observe VBN cord-328082-7c7slfbp 121 49 in in IN cord-328082-7c7slfbp 121 50 the the DT cord-328082-7c7slfbp 121 51 control control NN cord-328082-7c7slfbp 121 52 cells cell NNS cord-328082-7c7slfbp 121 53 fixed fix VBN cord-328082-7c7slfbp 121 54 at at IN cord-328082-7c7slfbp 121 55 the the DT cord-328082-7c7slfbp 121 56 restrictive restrictive JJ cord-328082-7c7slfbp 121 57 temperature temperature NN cord-328082-7c7slfbp 121 58 ( ( -LRB- cord-328082-7c7slfbp 121 59 38.5~ 38.5~ CD cord-328082-7c7slfbp 121 60 ( ( -LRB- cord-328082-7c7slfbp 121 61 Fig Fig NNP cord-328082-7c7slfbp 121 62 . . NNP cord-328082-7c7slfbp 121 63 2 2 CD cord-328082-7c7slfbp 121 64 a a CD cord-328082-7c7slfbp 121 65 ) ) -RRB- cord-328082-7c7slfbp 121 66 . . . cord-328082-7c7slfbp 122 1 At at IN cord-328082-7c7slfbp 122 2 24~ 24~ CD cord-328082-7c7slfbp 122 3 only only RB cord-328082-7c7slfbp 122 4 a a DT cord-328082-7c7slfbp 122 5 few few JJ cord-328082-7c7slfbp 122 6 cells cell NNS cord-328082-7c7slfbp 122 7 showed show VBD cord-328082-7c7slfbp 122 8 perinuclear perinuclear NNP cord-328082-7c7slfbp 122 9 vesicular vesicular JJ cord-328082-7c7slfbp 122 10 labeling labeling NN cord-328082-7c7slfbp 122 11 ( ( -LRB- cord-328082-7c7slfbp 122 12 Fig Fig NNP cord-328082-7c7slfbp 122 13 . . . cord-328082-7c7slfbp 122 14 2 2 CD cord-328082-7c7slfbp 122 15 f f NN cord-328082-7c7slfbp 122 16 , , , cord-328082-7c7slfbp 122 17 arrow arrow NN cord-328082-7c7slfbp 122 18 ) ) -RRB- cord-328082-7c7slfbp 122 19 in in IN cord-328082-7c7slfbp 122 20 addition addition NN cord-328082-7c7slfbp 122 21 to to IN cord-328082-7c7slfbp 122 22 ER ER NNP cord-328082-7c7slfbp 122 23 labeling labeling NN cord-328082-7c7slfbp 122 24 . . . cord-328082-7c7slfbp 123 1 At at IN cord-328082-7c7slfbp 123 2 25~ 25~ CD cord-328082-7c7slfbp 123 3 the the DT cord-328082-7c7slfbp 123 4 number number NN cord-328082-7c7slfbp 123 5 of of IN cord-328082-7c7slfbp 123 6 cells cell NNS cord-328082-7c7slfbp 123 7 displaying display VBG cord-328082-7c7slfbp 123 8 a a DT cord-328082-7c7slfbp 123 9 perinuclear perinuclear JJ cord-328082-7c7slfbp 123 10 pattern pattern NN cord-328082-7c7slfbp 123 11 of of IN cord-328082-7c7slfbp 123 12 labeling labeling NN cord-328082-7c7slfbp 123 13 increased increase VBD cord-328082-7c7slfbp 123 14 significantly significantly RB cord-328082-7c7slfbp 123 15 compared compare VBN cord-328082-7c7slfbp 123 16 with with IN cord-328082-7c7slfbp 123 17 the the DT cord-328082-7c7slfbp 123 18 situation situation NN cord-328082-7c7slfbp 123 19 at at IN cord-328082-7c7slfbp 123 20 24~ 24~ CD cord-328082-7c7slfbp 124 1 ( ( -LRB- cord-328082-7c7slfbp 124 2 Fig Fig NNP cord-328082-7c7slfbp 124 3 . . NNP cord-328082-7c7slfbp 124 4 2 2 CD cord-328082-7c7slfbp 124 5 g g NN cord-328082-7c7slfbp 124 6 ) ) -RRB- cord-328082-7c7slfbp 124 7 . . . cord-328082-7c7slfbp 125 1 As as IN cord-328082-7c7slfbp 125 2 was be VBD cord-328082-7c7slfbp 125 3 previously previously RB cord-328082-7c7slfbp 125 4 reported report VBN cord-328082-7c7slfbp 125 5 , , , cord-328082-7c7slfbp 125 6 the the DT cord-328082-7c7slfbp 125 7 SFV sfv NN cord-328082-7c7slfbp 125 8 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 125 9 accumulated accumulate VBN cord-328082-7c7slfbp 125 10 in in IN cord-328082-7c7slfbp 125 11 perinuclear perinuclear JJ cord-328082-7c7slfbp 125 12 structures structure NNS cord-328082-7c7slfbp 125 13 at at IN cord-328082-7c7slfbp 125 14 28~ 28~ CD cord-328082-7c7slfbp 125 15 in in IN cord-328082-7c7slfbp 125 16 the the DT cord-328082-7c7slfbp 125 17 presence presence NN cord-328082-7c7slfbp 125 18 of of IN cord-328082-7c7slfbp 125 19 caffeine caffeine NN cord-328082-7c7slfbp 125 20 ( ( -LRB- cord-328082-7c7slfbp 125 21 Fig Fig NNP cord-328082-7c7slfbp 125 22 . . NNP cord-328082-7c7slfbp 125 23 2 2 CD cord-328082-7c7slfbp 125 24 h h NN cord-328082-7c7slfbp 125 25 ) ) -RRB- cord-328082-7c7slfbp 125 26 . . . cord-328082-7c7slfbp 126 1 In in IN cord-328082-7c7slfbp 126 2 control control NN cord-328082-7c7slfbp 126 3 cells cell NNS cord-328082-7c7slfbp 126 4 , , , cord-328082-7c7slfbp 126 5 the the DT cord-328082-7c7slfbp 126 6 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 126 7 were be VBD cord-328082-7c7slfbp 126 8 transported transport VBN cord-328082-7c7slfbp 126 9 to to IN cord-328082-7c7slfbp 126 10 the the DT cord-328082-7c7slfbp 126 11 trans trans NNP cord-328082-7c7slfbp 126 12 - - NNP cord-328082-7c7slfbp 126 13 Golgi Golgi NNP cord-328082-7c7slfbp 126 14 region region NN cord-328082-7c7slfbp 126 15 already already RB cord-328082-7c7slfbp 126 16 at at IN cord-328082-7c7slfbp 126 17 200C 200C NNP cord-328082-7c7slfbp 126 18 as as IN cord-328082-7c7slfbp 126 19 described describe VBN cord-328082-7c7slfbp 126 20 earlier early RBR cord-328082-7c7slfbp 126 21 ( ( -LRB- cord-328082-7c7slfbp 126 22 Marlin Marlin NNP cord-328082-7c7slfbp 126 23 and and CC cord-328082-7c7slfbp 126 24 Simons Simons NNP cord-328082-7c7slfbp 126 25 , , , cord-328082-7c7slfbp 126 26 1983 1983 CD cord-328082-7c7slfbp 126 27 ; ; : cord-328082-7c7slfbp 126 28 Saraste Saraste NNP cord-328082-7c7slfbp 126 29 and and CC cord-328082-7c7slfbp 126 30 Kuismanen Kuismanen NNP cord-328082-7c7slfbp 126 31 , , , cord-328082-7c7slfbp 126 32 1984 1984 CD cord-328082-7c7slfbp 126 33 ) ) -RRB- cord-328082-7c7slfbp 126 34 , , , cord-328082-7c7slfbp 126 35 and and CC cord-328082-7c7slfbp 126 36 at at IN cord-328082-7c7slfbp 126 37 higher high JJR cord-328082-7c7slfbp 126 38 temperatures temperature NNS cord-328082-7c7slfbp 126 39 increased increase VBD cord-328082-7c7slfbp 126 40 labeling labeling NN cord-328082-7c7slfbp 126 41 of of IN cord-328082-7c7slfbp 126 42 the the DT cord-328082-7c7slfbp 126 43 plasma plasma NN cord-328082-7c7slfbp 126 44 membrane membrane NN cord-328082-7c7slfbp 126 45 was be VBD cord-328082-7c7slfbp 126 46 observed observe VBN cord-328082-7c7slfbp 126 47 ( ( -LRB- cord-328082-7c7slfbp 126 48 data datum NNS cord-328082-7c7slfbp 126 49 not not RB cord-328082-7c7slfbp 126 50 shown show VBN cord-328082-7c7slfbp 126 51 ) ) -RRB- cord-328082-7c7slfbp 126 52 . . . cord-328082-7c7slfbp 127 1 These these DT cord-328082-7c7slfbp 127 2 biochemical biochemical JJ cord-328082-7c7slfbp 127 3 and and CC cord-328082-7c7slfbp 127 4 morphological morphological JJ cord-328082-7c7slfbp 127 5 experiments experiment NNS cord-328082-7c7slfbp 127 6 suggest suggest VBP cord-328082-7c7slfbp 127 7 that that IN cord-328082-7c7slfbp 127 8 in in IN cord-328082-7c7slfbp 127 9 the the DT cord-328082-7c7slfbp 127 10 presence presence NN cord-328082-7c7slfbp 127 11 of of IN cord-328082-7c7slfbp 127 12 10 10 CD cord-328082-7c7slfbp 127 13 mM mm CD cord-328082-7c7slfbp 127 14 caffeine caffeine NN cord-328082-7c7slfbp 127 15 , , , cord-328082-7c7slfbp 127 16 SFV sfv NN cord-328082-7c7slfbp 127 17 E1 e1 NN cord-328082-7c7slfbp 127 18 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 127 19 are be VBP cord-328082-7c7slfbp 127 20 retained retain VBN cord-328082-7c7slfbp 127 21 in in IN cord-328082-7c7slfbp 127 22 the the DT cord-328082-7c7slfbp 127 23 ER er NN cord-328082-7c7slfbp 127 24 up up IN cord-328082-7c7slfbp 127 25 to to IN cord-328082-7c7slfbp 127 26 230C 230c CD cord-328082-7c7slfbp 127 27 , , , cord-328082-7c7slfbp 127 28 whereas whereas IN cord-328082-7c7slfbp 127 29 at at IN cord-328082-7c7slfbp 127 30 250C 250c CD cord-328082-7c7slfbp 127 31 they -PRON- PRP cord-328082-7c7slfbp 127 32 reach reach VBP cord-328082-7c7slfbp 127 33 the the DT cord-328082-7c7slfbp 127 34 Golgi Golgi NNP cord-328082-7c7slfbp 127 35 stack stack NN cord-328082-7c7slfbp 127 36 . . . cord-328082-7c7slfbp 128 1 The the DT cord-328082-7c7slfbp 128 2 experiments experiment NNS cord-328082-7c7slfbp 128 3 may may MD cord-328082-7c7slfbp 128 4 further further RB cord-328082-7c7slfbp 128 5 indicate indicate VB cord-328082-7c7slfbp 128 6 that that IN cord-328082-7c7slfbp 128 7 SFV sfv NN cord-328082-7c7slfbp 128 8 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 128 9 are be VBP cord-328082-7c7slfbp 128 10 able able JJ cord-328082-7c7slfbp 128 11 , , , cord-328082-7c7slfbp 128 12 at at IN cord-328082-7c7slfbp 128 13 least least JJS cord-328082-7c7slfbp 128 14 in in IN cord-328082-7c7slfbp 128 15 some some DT cord-328082-7c7slfbp 128 16 degree degree NN cord-328082-7c7slfbp 128 17 , , , cord-328082-7c7slfbp 128 18 to to TO cord-328082-7c7slfbp 128 19 escape escape VB cord-328082-7c7slfbp 128 20 to to IN cord-328082-7c7slfbp 128 21 peripheral peripheral JJ cord-328082-7c7slfbp 128 22 vesicular vesicular JJ cord-328082-7c7slfbp 128 23 structures structure NNS cord-328082-7c7slfbp 128 24 already already RB cord-328082-7c7slfbp 128 25 at at IN cord-328082-7c7slfbp 128 26 240C 240c CD cord-328082-7c7slfbp 128 27 when when WRB cord-328082-7c7slfbp 128 28 still still RB cord-328082-7c7slfbp 128 29 possessing possess VBG cord-328082-7c7slfbp 128 30 clearly clearly RB cord-328082-7c7slfbp 128 31 Endo Endo NNP cord-328082-7c7slfbp 128 32 H h NN cord-328082-7c7slfbp 128 33 - - HYPH cord-328082-7c7slfbp 128 34 sensitive sensitive JJ cord-328082-7c7slfbp 128 35 glycans glycan NNS cord-328082-7c7slfbp 128 36 . . . cord-328082-7c7slfbp 129 1 However however RB cord-328082-7c7slfbp 129 2 , , , cord-328082-7c7slfbp 129 3 to to TO cord-328082-7c7slfbp 129 4 further further RB cord-328082-7c7slfbp 129 5 clarify clarify VB cord-328082-7c7slfbp 129 6 this this DT cord-328082-7c7slfbp 129 7 issue issue NN cord-328082-7c7slfbp 129 8 , , , cord-328082-7c7slfbp 129 9 morphological morphological JJ cord-328082-7c7slfbp 129 10 colocalization colocalization NN cord-328082-7c7slfbp 129 11 experiments experiment NNS cord-328082-7c7slfbp 129 12 with with IN cord-328082-7c7slfbp 129 13 a a DT cord-328082-7c7slfbp 129 14 marker marker NN cord-328082-7c7slfbp 129 15 to to IN cord-328082-7c7slfbp 129 16 the the DT cord-328082-7c7slfbp 129 17 pre pre JJ cord-328082-7c7slfbp 129 18 - - JJ cord-328082-7c7slfbp 129 19 Golgi golgi JJ cord-328082-7c7slfbp 129 20 membranes membrane NNS cord-328082-7c7slfbp 129 21 will will MD cord-328082-7c7slfbp 129 22 be be VB cord-328082-7c7slfbp 129 23 necessary necessary JJ cord-328082-7c7slfbp 129 24 . . . cord-328082-7c7slfbp 130 1 The the DT cord-328082-7c7slfbp 130 2 observed observed JJ cord-328082-7c7slfbp 130 3 inhibition inhibition NN cord-328082-7c7slfbp 130 4 of of IN cord-328082-7c7slfbp 130 5 ER er NN cord-328082-7c7slfbp 130 6 - - HYPH cord-328082-7c7slfbp 130 7 exit exit NN cord-328082-7c7slfbp 130 8 of of IN cord-328082-7c7slfbp 130 9 SFV sfv NN cord-328082-7c7slfbp 130 10 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 130 11 could could MD cord-328082-7c7slfbp 130 12 be be VB cord-328082-7c7slfbp 130 13 a a DT cord-328082-7c7slfbp 130 14 result result NN cord-328082-7c7slfbp 130 15 of of IN cord-328082-7c7slfbp 130 16 not not RB cord-328082-7c7slfbp 130 17 only only RB cord-328082-7c7slfbp 130 18 the the DT cord-328082-7c7slfbp 130 19 inhibition inhibition NN cord-328082-7c7slfbp 130 20 of of IN cord-328082-7c7slfbp 130 21 membrane membrane NN cord-328082-7c7slfbp 130 22 traffic traffic NN cord-328082-7c7slfbp 130 23 , , , cord-328082-7c7slfbp 130 24 but but CC cord-328082-7c7slfbp 130 25 also also RB cord-328082-7c7slfbp 130 26 other other JJ cord-328082-7c7slfbp 130 27 factors factor NNS cord-328082-7c7slfbp 130 28 , , , cord-328082-7c7slfbp 130 29 such such JJ cord-328082-7c7slfbp 130 30 as as IN cord-328082-7c7slfbp 130 31 inhibition inhibition NN cord-328082-7c7slfbp 130 32 of of IN cord-328082-7c7slfbp 130 33 proper proper JJ cord-328082-7c7slfbp 130 34 folding folding NN cord-328082-7c7slfbp 130 35 and and CC cord-328082-7c7slfbp 130 36 oligomerization oligomerization NN cord-328082-7c7slfbp 130 37 , , , cord-328082-7c7slfbp 130 38 known know VBN cord-328082-7c7slfbp 130 39 to to TO cord-328082-7c7slfbp 130 40 control control VB cord-328082-7c7slfbp 130 41 the the DT cord-328082-7c7slfbp 130 42 ER er NN cord-328082-7c7slfbp 130 43 - - HYPH cord-328082-7c7slfbp 130 44 exit exit NN cord-328082-7c7slfbp 130 45 of of IN cord-328082-7c7slfbp 130 46 individual individual JJ cord-328082-7c7slfbp 130 47 proteins protein NNS cord-328082-7c7slfbp 130 48 ( ( -LRB- cord-328082-7c7slfbp 130 49 Rose Rose NNP cord-328082-7c7slfbp 130 50 and and CC cord-328082-7c7slfbp 130 51 Doms Doms NNP cord-328082-7c7slfbp 130 52 , , , cord-328082-7c7slfbp 130 53 1988 1988 CD cord-328082-7c7slfbp 130 54 ; ; : cord-328082-7c7slfbp 130 55 Helenius Helenius NNP cord-328082-7c7slfbp 130 56 et et NNP cord-328082-7c7slfbp 130 57 al al NNP cord-328082-7c7slfbp 130 58 . . NNP cord-328082-7c7slfbp 130 59 , , , cord-328082-7c7slfbp 130 60 1992 1992 CD cord-328082-7c7slfbp 130 61 ) ) -RRB- cord-328082-7c7slfbp 130 62 . . . cord-328082-7c7slfbp 131 1 To to TO cord-328082-7c7slfbp 131 2 test test VB cord-328082-7c7slfbp 131 3 the the DT cord-328082-7c7slfbp 131 4 effect effect NN cord-328082-7c7slfbp 131 5 of of IN cord-328082-7c7slfbp 131 6 10 10 CD cord-328082-7c7slfbp 131 7 mM mm CD cord-328082-7c7slfbp 131 8 caffeine caffeine NN cord-328082-7c7slfbp 131 9 and and CC cord-328082-7c7slfbp 131 10 20~ 20~ CD cord-328082-7c7slfbp 131 11 on on IN cord-328082-7c7slfbp 131 12 the the DT cord-328082-7c7slfbp 131 13 movement movement NN cord-328082-7c7slfbp 131 14 of of IN cord-328082-7c7slfbp 131 15 cellular cellular JJ cord-328082-7c7slfbp 131 16 proteins protein NNS cord-328082-7c7slfbp 131 17 , , , cord-328082-7c7slfbp 131 18 we -PRON- PRP cord-328082-7c7slfbp 131 19 followed follow VBD cord-328082-7c7slfbp 131 20 the the DT cord-328082-7c7slfbp 131 21 recovery recovery NN cord-328082-7c7slfbp 131 22 of of IN cord-328082-7c7slfbp 131 23 Golgi Golgi NNP cord-328082-7c7slfbp 131 24 membranes membrane NNS cord-328082-7c7slfbp 131 25 from from IN cord-328082-7c7slfbp 131 26 BFA BFA NNP cord-328082-7c7slfbp 131 27 treatment treatment NN cord-328082-7c7slfbp 131 28 using use VBG cord-328082-7c7slfbp 131 29 as as IN cord-328082-7c7slfbp 131 30 a a DT cord-328082-7c7slfbp 131 31 marker marker NN cord-328082-7c7slfbp 131 32 man man NN cord-328082-7c7slfbp 131 33 II II NNP cord-328082-7c7slfbp 131 34 , , , cord-328082-7c7slfbp 131 35 a a DT cord-328082-7c7slfbp 131 36 resident resident JJ cord-328082-7c7slfbp 131 37 Golgi Golgi NNP cord-328082-7c7slfbp 131 38 membrane membrane NN cord-328082-7c7slfbp 131 39 protein protein NN cord-328082-7c7slfbp 131 40 . . . cord-328082-7c7slfbp 132 1 BFA BFA NNP cord-328082-7c7slfbp 132 2 has have VBZ cord-328082-7c7slfbp 132 3 recenrly recenrly RB cord-328082-7c7slfbp 132 4 been be VBN cord-328082-7c7slfbp 132 5 shown show VBN cord-328082-7c7slfbp 132 6 to to TO cord-328082-7c7slfbp 132 7 cause cause VB cord-328082-7c7slfbp 132 8 the the DT cord-328082-7c7slfbp 132 9 redistribution redistribution NN cord-328082-7c7slfbp 132 10 of of IN cord-328082-7c7slfbp 132 11 man man NN cord-328082-7c7slfbp 132 12 II II NNP cord-328082-7c7slfbp 132 13 and and CC cord-328082-7c7slfbp 132 14 other other JJ cord-328082-7c7slfbp 132 15 resident resident NN cord-328082-7c7slfbp 132 16 Golgi Golgi NNP cord-328082-7c7slfbp 132 17 proteins protein NNS cord-328082-7c7slfbp 132 18 as as RB cord-328082-7c7slfbp 132 19 well well RB cord-328082-7c7slfbp 132 20 as as IN cord-328082-7c7slfbp 132 21 Golgi Golgi NNP cord-328082-7c7slfbp 132 22 membranes membrane NNS cord-328082-7c7slfbp 132 23 to to IN cord-328082-7c7slfbp 132 24 the the DT cord-328082-7c7slfbp 132 25 ER ER NNP cord-328082-7c7slfbp 132 26 in in IN cord-328082-7c7slfbp 132 27 a a DT cord-328082-7c7slfbp 132 28 reversible reversible JJ cord-328082-7c7slfbp 132 29 manner manner NN cord-328082-7c7slfbp 132 30 ( ( -LRB- cord-328082-7c7slfbp 132 31 Doms Doms NNP cord-328082-7c7slfbp 132 32 et et FW cord-328082-7c7slfbp 132 33 al al NNP cord-328082-7c7slfbp 132 34 . . . cord-328082-7c7slfbp 133 1 , , , cord-328082-7c7slfbp 133 2 1989 1989 CD cord-328082-7c7slfbp 133 3 ; ; : cord-328082-7c7slfbp 133 4 Fujiwara Fujiwara NNP cord-328082-7c7slfbp 133 5 et et NNP cord-328082-7c7slfbp 133 6 al al NNP cord-328082-7c7slfbp 133 7 . . NNP cord-328082-7c7slfbp 133 8 , , , cord-328082-7c7slfbp 133 9 1988 1988 CD cord-328082-7c7slfbp 133 10 ; ; : cord-328082-7c7slfbp 133 11 Lippincott Lippincott NNP cord-328082-7c7slfbp 133 12 - - HYPH cord-328082-7c7slfbp 133 13 Schwartz Schwartz NNP cord-328082-7c7slfbp 133 14 et et NNP cord-328082-7c7slfbp 133 15 al al NNP cord-328082-7c7slfbp 133 16 . . NNP cord-328082-7c7slfbp 133 17 , , , cord-328082-7c7slfbp 133 18 1989 1989 CD cord-328082-7c7slfbp 134 1 , , , cord-328082-7c7slfbp 134 2 1991a 1991a CD cord-328082-7c7slfbp 134 3 Strous Strous NNP cord-328082-7c7slfbp 134 4 et et NNP cord-328082-7c7slfbp 134 5 al al NNP cord-328082-7c7slfbp 134 6 . . NNP cord-328082-7c7slfbp 134 7 , , , cord-328082-7c7slfbp 134 8 1991 1991 CD cord-328082-7c7slfbp 134 9 ) ) -RRB- cord-328082-7c7slfbp 134 10 . . . cord-328082-7c7slfbp 135 1 BHK-21 bhk-21 DT cord-328082-7c7slfbp 135 2 cells cell NNS cord-328082-7c7slfbp 135 3 were be VBD cord-328082-7c7slfbp 135 4 treated treat VBN cord-328082-7c7slfbp 135 5 for for IN cord-328082-7c7slfbp 135 6 90 90 CD cord-328082-7c7slfbp 135 7 min min NN cord-328082-7c7slfbp 135 8 with with IN cord-328082-7c7slfbp 135 9 2 2 CD cord-328082-7c7slfbp 135 10 # # NN cord-328082-7c7slfbp 135 11 g g NN cord-328082-7c7slfbp 135 12 / / SYM cord-328082-7c7slfbp 135 13 ml ml NN cord-328082-7c7slfbp 135 14 of of IN cord-328082-7c7slfbp 135 15 BFA BFA NNP cord-328082-7c7slfbp 135 16 at at IN cord-328082-7c7slfbp 135 17 37~ 37~ CD cord-328082-7c7slfbp 135 18 to to TO cord-328082-7c7slfbp 135 19 ensure ensure VB cord-328082-7c7slfbp 135 20 complete complete JJ cord-328082-7c7slfbp 135 21 translocation translocation NN cord-328082-7c7slfbp 135 22 of of IN cord-328082-7c7slfbp 135 23 man man NN cord-328082-7c7slfbp 135 24 II II NNP cord-328082-7c7slfbp 135 25 to to IN cord-328082-7c7slfbp 135 26 the the DT cord-328082-7c7slfbp 135 27 ER er NN cord-328082-7c7slfbp 135 28 . . . cord-328082-7c7slfbp 136 1 To to TO cord-328082-7c7slfbp 136 2 follow follow VB cord-328082-7c7slfbp 136 3 the the DT cord-328082-7c7slfbp 136 4 movement movement NN cord-328082-7c7slfbp 136 5 of of IN cord-328082-7c7slfbp 136 6 man man NNP cord-328082-7c7slfbp 136 7 II II NNP cord-328082-7c7slfbp 136 8 out out IN cord-328082-7c7slfbp 136 9 of of IN cord-328082-7c7slfbp 136 10 the the DT cord-328082-7c7slfbp 136 11 ER ER NNP cord-328082-7c7slfbp 136 12 , , , cord-328082-7c7slfbp 136 13 BFA BFA NNP cord-328082-7c7slfbp 136 14 was be VBD cord-328082-7c7slfbp 136 15 removed remove VBN cord-328082-7c7slfbp 136 16 with with IN cord-328082-7c7slfbp 136 17 several several JJ cord-328082-7c7slfbp 136 18 washes wash NNS cord-328082-7c7slfbp 136 19 of of IN cord-328082-7c7slfbp 136 20 preconditioned precondition VBN cord-328082-7c7slfbp 136 21 medium medium NN cord-328082-7c7slfbp 136 22 ( ( -LRB- cord-328082-7c7slfbp 136 23 20~ 20~ CD cord-328082-7c7slfbp 136 24 in in IN cord-328082-7c7slfbp 136 25 the the DT cord-328082-7c7slfbp 136 26 presence presence NN cord-328082-7c7slfbp 136 27 or or CC cord-328082-7c7slfbp 136 28 absence absence NN cord-328082-7c7slfbp 136 29 of of IN cord-328082-7c7slfbp 136 30 10 10 CD cord-328082-7c7slfbp 136 31 mM mm CD cord-328082-7c7slfbp 136 32 caffeine caffeine NN cord-328082-7c7slfbp 136 33 . . . cord-328082-7c7slfbp 137 1 Thereafter thereafter RB cord-328082-7c7slfbp 137 2 , , , cord-328082-7c7slfbp 137 3 the the DT cord-328082-7c7slfbp 137 4 cells cell NNS cord-328082-7c7slfbp 137 5 were be VBD cord-328082-7c7slfbp 137 6 chased chase VBN cord-328082-7c7slfbp 137 7 for for IN cord-328082-7c7slfbp 137 8 60 60 CD cord-328082-7c7slfbp 137 9 , , , cord-328082-7c7slfbp 137 10 120 120 CD cord-328082-7c7slfbp 137 11 , , , cord-328082-7c7slfbp 137 12 and and CC cord-328082-7c7slfbp 137 13 180 180 CD cord-328082-7c7slfbp 137 14 min min NN cord-328082-7c7slfbp 137 15 at at IN cord-328082-7c7slfbp 137 16 20~ 20~ CD cord-328082-7c7slfbp 137 17 with with IN cord-328082-7c7slfbp 137 18 ( ( -LRB- cord-328082-7c7slfbp 137 19 Fig Fig NNP cord-328082-7c7slfbp 137 20 . . . cord-328082-7c7slfbp 137 21 3 3 CD cord-328082-7c7slfbp 137 22 , , , cord-328082-7c7slfbp 137 23 d d NNP cord-328082-7c7slfbp 137 24 , , , cord-328082-7c7slfbp 137 25 f f NNP cord-328082-7c7slfbp 137 26 , , , cord-328082-7c7slfbp 137 27 and and CC cord-328082-7c7slfbp 137 28 h h NNP cord-328082-7c7slfbp 137 29 , , , cord-328082-7c7slfbp 137 30 respectively respectively RB cord-328082-7c7slfbp 137 31 ) ) -RRB- cord-328082-7c7slfbp 137 32 or or CC cord-328082-7c7slfbp 137 33 without without IN cord-328082-7c7slfbp 137 34 ( ( -LRB- cord-328082-7c7slfbp 137 35 Fig Fig NNP cord-328082-7c7slfbp 137 36 . . . cord-328082-7c7slfbp 137 37 3 3 CD cord-328082-7c7slfbp 137 38 , , , cord-328082-7c7slfbp 137 39 c c NNP cord-328082-7c7slfbp 137 40 , , , cord-328082-7c7slfbp 137 41 e e NNP cord-328082-7c7slfbp 137 42 , , , cord-328082-7c7slfbp 137 43 and and CC cord-328082-7c7slfbp 137 44 g g NN cord-328082-7c7slfbp 137 45 , , , cord-328082-7c7slfbp 137 46 respectively respectively RB cord-328082-7c7slfbp 137 47 ) ) -RRB- cord-328082-7c7slfbp 137 48 caffeine caffeine NN cord-328082-7c7slfbp 137 49 , , , cord-328082-7c7slfbp 137 50 and and CC cord-328082-7c7slfbp 137 51 then then RB cord-328082-7c7slfbp 137 52 fixed fix VBN cord-328082-7c7slfbp 137 53 and and CC cord-328082-7c7slfbp 137 54 prepared prepare VBN cord-328082-7c7slfbp 137 55 for for IN cord-328082-7c7slfbp 137 56 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 137 57 . . . cord-328082-7c7slfbp 138 1 In in IN cord-328082-7c7slfbp 138 2 Fig Fig NNP cord-328082-7c7slfbp 138 3 . . . cord-328082-7c7slfbp 138 4 _SP cord-328082-7c7slfbp 139 1 3 3 LS cord-328082-7c7slfbp 139 2 a a DT cord-328082-7c7slfbp 140 1 , , , cord-328082-7c7slfbp 140 2 the the DT cord-328082-7c7slfbp 140 3 distribution distribution NN cord-328082-7c7slfbp 140 4 of of IN cord-328082-7c7slfbp 140 5 man man NN cord-328082-7c7slfbp 140 6 II II NNP cord-328082-7c7slfbp 140 7 is be VBZ cord-328082-7c7slfbp 140 8 shown show VBN cord-328082-7c7slfbp 140 9 before before IN cord-328082-7c7slfbp 140 10 any any DT cord-328082-7c7slfbp 140 11 drug drug NN cord-328082-7c7slfbp 140 12 treatments treatment NNS cord-328082-7c7slfbp 140 13 . . . cord-328082-7c7slfbp 141 1 In in IN cord-328082-7c7slfbp 141 2 cells cell NNS cord-328082-7c7slfbp 141 3 treated treat VBN cord-328082-7c7slfbp 141 4 for for IN cord-328082-7c7slfbp 141 5 90 90 CD cord-328082-7c7slfbp 141 6 min min NN cord-328082-7c7slfbp 141 7 with with IN cord-328082-7c7slfbp 141 8 BFA BFA NNP cord-328082-7c7slfbp 141 9 at at IN cord-328082-7c7slfbp 141 10 370C 370C NNP cord-328082-7c7slfbp 141 11 , , , cord-328082-7c7slfbp 141 12 man man NN cord-328082-7c7slfbp 141 13 Figure figure NN cord-328082-7c7slfbp 141 14 2 2 CD cord-328082-7c7slfbp 141 15 . . . cord-328082-7c7slfbp 142 1 The the DT cord-328082-7c7slfbp 142 2 exit exit NN cord-328082-7c7slfbp 142 3 of of IN cord-328082-7c7slfbp 142 4 SFV sfv NN cord-328082-7c7slfbp 142 5 membrane membrane NN cord-328082-7c7slfbp 142 6 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 142 7 E1 e1 NN cord-328082-7c7slfbp 142 8 and and CC cord-328082-7c7slfbp 142 9 E2 E2 NNP cord-328082-7c7slfbp 142 10 from from IN cord-328082-7c7slfbp 142 11 the the DT cord-328082-7c7slfbp 142 12 ER ER NNP cord-328082-7c7slfbp 142 13 in in IN cord-328082-7c7slfbp 142 14 the the DT cord-328082-7c7slfbp 142 15 presence presence NN cord-328082-7c7slfbp 142 16 of of IN cord-328082-7c7slfbp 142 17 10 10 CD cord-328082-7c7slfbp 142 18 mM mm CD cord-328082-7c7slfbp 142 19 caffeine caffeine NN cord-328082-7c7slfbp 142 20 at at IN cord-328082-7c7slfbp 142 21 different different JJ cord-328082-7c7slfbp 142 22 temperatures temperature NNS cord-328082-7c7slfbp 142 23 . . . cord-328082-7c7slfbp 143 1 BHK-21 bhk-21 DT cord-328082-7c7slfbp 143 2 ceils ceil VBZ cord-328082-7c7slfbp 143 3 infected infect VBN cord-328082-7c7slfbp 143 4 with with IN cord-328082-7c7slfbp 143 5 SFV SFV NNP cord-328082-7c7slfbp 143 6 ts-1 ts-1 NNP cord-328082-7c7slfbp 143 7 were be VBD cord-328082-7c7slfbp 143 8 incubated incubate VBN cord-328082-7c7slfbp 143 9 for for IN cord-328082-7c7slfbp 143 10 120 120 CD cord-328082-7c7slfbp 143 11 min min NNS cord-328082-7c7slfbp 143 12 in in IN cord-328082-7c7slfbp 143 13 the the DT cord-328082-7c7slfbp 143 14 presence presence NN cord-328082-7c7slfbp 143 15 of of IN cord-328082-7c7slfbp 143 16 10 10 CD cord-328082-7c7slfbp 143 17 mM mm CD cord-328082-7c7slfbp 143 18 caffeine caffeine NN cord-328082-7c7slfbp 143 19 and and CC cord-328082-7c7slfbp 143 20 50 50 CD cord-328082-7c7slfbp 143 21 # # NN cord-328082-7c7slfbp 143 22 g g NN cord-328082-7c7slfbp 143 23 / / SYM cord-328082-7c7slfbp 143 24 ml ml NN cord-328082-7c7slfbp 143 25 of of IN cord-328082-7c7slfbp 143 26 cycloheximide cycloheximide NN cord-328082-7c7slfbp 143 27 at at IN cord-328082-7c7slfbp 143 28 20 20 CD cord-328082-7c7slfbp 143 29 ( ( -LRB- cord-328082-7c7slfbp 143 30 b b NN cord-328082-7c7slfbp 143 31 ) ) -RRB- cord-328082-7c7slfbp 143 32 , , , cord-328082-7c7slfbp 143 33 21 21 CD cord-328082-7c7slfbp 143 34 ( ( -LRB- cord-328082-7c7slfbp 143 35 c c NN cord-328082-7c7slfbp 143 36 ) ) -RRB- cord-328082-7c7slfbp 143 37 , , , cord-328082-7c7slfbp 143 38 22 22 CD cord-328082-7c7slfbp 143 39 ( ( -LRB- cord-328082-7c7slfbp 143 40 d d NN cord-328082-7c7slfbp 143 41 ) ) -RRB- cord-328082-7c7slfbp 143 42 , , , cord-328082-7c7slfbp 143 43 23 23 CD cord-328082-7c7slfbp 143 44 ( ( -LRB- cord-328082-7c7slfbp 143 45 e e NN cord-328082-7c7slfbp 143 46 ) ) -RRB- cord-328082-7c7slfbp 143 47 , , , cord-328082-7c7slfbp 143 48 24 24 CD cord-328082-7c7slfbp 143 49 ( ( -LRB- cord-328082-7c7slfbp 143 50 f f NNP cord-328082-7c7slfbp 143 51 ) ) -RRB- cord-328082-7c7slfbp 143 52 , , , cord-328082-7c7slfbp 143 53 25 25 CD cord-328082-7c7slfbp 143 54 ( ( -LRB- cord-328082-7c7slfbp 143 55 g g NNP cord-328082-7c7slfbp 143 56 ) ) -RRB- cord-328082-7c7slfbp 143 57 , , , cord-328082-7c7slfbp 143 58 and and CC cord-328082-7c7slfbp 143 59 28~ 28~ CD cord-328082-7c7slfbp 143 60 ( ( -LRB- cord-328082-7c7slfbp 143 61 h h NN cord-328082-7c7slfbp 143 62 ) ) -RRB- cord-328082-7c7slfbp 143 63 , , , cord-328082-7c7slfbp 143 64 fixed fix VBN cord-328082-7c7slfbp 143 65 and and CC cord-328082-7c7slfbp 143 66 processed process VBN cord-328082-7c7slfbp 143 67 for for IN cord-328082-7c7slfbp 143 68 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 143 69 , , , cord-328082-7c7slfbp 143 70 a a DT cord-328082-7c7slfbp 143 71 shows show VBZ cord-328082-7c7slfbp 143 72 the the DT cord-328082-7c7slfbp 143 73 distribution distribution NN cord-328082-7c7slfbp 143 74 of of IN cord-328082-7c7slfbp 143 75 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 143 76 at at IN cord-328082-7c7slfbp 143 77 the the DT cord-328082-7c7slfbp 143 78 restrictive restrictive JJ cord-328082-7c7slfbp 143 79 temperature temperature NN cord-328082-7c7slfbp 143 80 38.5~ 38.5~ . cord-328082-7c7slfbp 144 1 At at IN cord-328082-7c7slfbp 144 2 temperatures temperature NNS cord-328082-7c7slfbp 144 3 20 20 CD cord-328082-7c7slfbp 144 4 , , , cord-328082-7c7slfbp 144 5 21 21 CD cord-328082-7c7slfbp 144 6 , , , cord-328082-7c7slfbp 144 7 22 22 CD cord-328082-7c7slfbp 144 8 , , , cord-328082-7c7slfbp 144 9 and and CC cord-328082-7c7slfbp 144 10 23~ 23~ CD cord-328082-7c7slfbp 144 11 ( ( -LRB- cord-328082-7c7slfbp 144 12 b b NN cord-328082-7c7slfbp 144 13 , , , cord-328082-7c7slfbp 144 14 c c NNP cord-328082-7c7slfbp 144 15 , , , cord-328082-7c7slfbp 144 16 d d NNP cord-328082-7c7slfbp 144 17 , , , cord-328082-7c7slfbp 144 18 and and CC cord-328082-7c7slfbp 144 19 e e NNP cord-328082-7c7slfbp 144 20 , , , cord-328082-7c7slfbp 144 21 respectively respectively RB cord-328082-7c7slfbp 144 22 ) ) -RRB- cord-328082-7c7slfbp 144 23 , , , cord-328082-7c7slfbp 144 24 SFV sfv NN cord-328082-7c7slfbp 144 25 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 144 26 can can MD cord-328082-7c7slfbp 144 27 be be VB cord-328082-7c7slfbp 144 28 found find VBN cord-328082-7c7slfbp 144 29 in in IN cord-328082-7c7slfbp 144 30 the the DT cord-328082-7c7slfbp 144 31 ER ER NNP cord-328082-7c7slfbp 144 32 . . . cord-328082-7c7slfbp 145 1 At at IN cord-328082-7c7slfbp 145 2 240C 240c CD cord-328082-7c7slfbp 145 3 ( ( -LRB- cord-328082-7c7slfbp 145 4 f f NNP cord-328082-7c7slfbp 145 5 ) ) -RRB- cord-328082-7c7slfbp 145 6 , , , cord-328082-7c7slfbp 145 7 a a DT cord-328082-7c7slfbp 145 8 few few JJ cord-328082-7c7slfbp 145 9 cells cell NNS cord-328082-7c7slfbp 145 10 possess possess VBP cord-328082-7c7slfbp 145 11 also also RB cord-328082-7c7slfbp 145 12 vesicular vesicular JJ cord-328082-7c7slfbp 145 13 labeling labeling NN cord-328082-7c7slfbp 145 14 ~ ~ NFP cord-328082-7c7slfbp 145 15 arrow arrow NN cord-328082-7c7slfbp 145 16 ) ) -RRB- cord-328082-7c7slfbp 145 17 . . . cord-328082-7c7slfbp 146 1 At at IN cord-328082-7c7slfbp 146 2 25~ 25~ CD cord-328082-7c7slfbp 146 3 in in IN cord-328082-7c7slfbp 146 4 more more JJR cord-328082-7c7slfbp 146 5 than than IN cord-328082-7c7slfbp 146 6 half half NN cord-328082-7c7slfbp 146 7 of of IN cord-328082-7c7slfbp 146 8 the the DT cord-328082-7c7slfbp 146 9 cells cell NNS cord-328082-7c7slfbp 146 10 , , , cord-328082-7c7slfbp 146 11 SFV sfv NN cord-328082-7c7slfbp 146 12 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 146 13 can can MD cord-328082-7c7slfbp 146 14 exit exit VB cord-328082-7c7slfbp 146 15 the the DT cord-328082-7c7slfbp 146 16 ER er NN cord-328082-7c7slfbp 146 17 and and CC cord-328082-7c7slfbp 146 18 accumulate accumulate VB cord-328082-7c7slfbp 146 19 to to IN cord-328082-7c7slfbp 146 20 perinuclear perinuclear NN cord-328082-7c7slfbp 146 21 structures structure NNS cord-328082-7c7slfbp 146 22 ( ( -LRB- cord-328082-7c7slfbp 146 23 g g NNP cord-328082-7c7slfbp 146 24 ) ) -RRB- cord-328082-7c7slfbp 146 25 . . . cord-328082-7c7slfbp 147 1 At at IN cord-328082-7c7slfbp 147 2 28~ 28~ CD cord-328082-7c7slfbp 147 3 virtually virtually RB cord-328082-7c7slfbp 147 4 all all DT cord-328082-7c7slfbp 147 5 cells cell NNS cord-328082-7c7slfbp 147 6 show show VBP cord-328082-7c7slfbp 147 7 perinuclear perinuclear NN cord-328082-7c7slfbp 147 8 localization localization NN cord-328082-7c7slfbp 147 9 of of IN cord-328082-7c7slfbp 147 10 El El NNP cord-328082-7c7slfbp 147 11 ( ( -LRB- cord-328082-7c7slfbp 147 12 h h NN cord-328082-7c7slfbp 147 13 ) ) -RRB- cord-328082-7c7slfbp 147 14 . . . cord-328082-7c7slfbp 148 1 Bar bar NN cord-328082-7c7slfbp 148 2 , , , cord-328082-7c7slfbp 148 3 20/~m 20/~m NNP cord-328082-7c7slfbp 148 4 . . . cord-328082-7c7slfbp 149 1 II II NNP cord-328082-7c7slfbp 149 2 is be VBZ cord-328082-7c7slfbp 149 3 translocated translocate VBN cord-328082-7c7slfbp 149 4 efficiently efficiently RB cord-328082-7c7slfbp 149 5 ( ( -LRB- cord-328082-7c7slfbp 149 6 in in IN cord-328082-7c7slfbp 149 7 99 99 CD cord-328082-7c7slfbp 149 8 % % NN cord-328082-7c7slfbp 149 9 of of IN cord-328082-7c7slfbp 149 10 the the DT cord-328082-7c7slfbp 149 11 cells cell NNS cord-328082-7c7slfbp 149 12 ) ) -RRB- cord-328082-7c7slfbp 149 13 to to IN cord-328082-7c7slfbp 149 14 the the DT cord-328082-7c7slfbp 149 15 ER ER NNP cord-328082-7c7slfbp 149 16 ( ( -LRB- cord-328082-7c7slfbp 149 17 Fig fig NN cord-328082-7c7slfbp 149 18 . . . cord-328082-7c7slfbp 149 19 3 3 CD cord-328082-7c7slfbp 149 20 b b NN cord-328082-7c7slfbp 149 21 ) ) -RRB- cord-328082-7c7slfbp 149 22 . . . cord-328082-7c7slfbp 150 1 As as IN cord-328082-7c7slfbp 150 2 the the DT cord-328082-7c7slfbp 150 3 cells cell NNS cord-328082-7c7slfbp 150 4 were be VBD cord-328082-7c7slfbp 150 5 incubated incubate VBN cord-328082-7c7slfbp 150 6 for for IN cord-328082-7c7slfbp 150 7 60 60 CD cord-328082-7c7slfbp 150 8 min min NN cord-328082-7c7slfbp 150 9 in in IN cord-328082-7c7slfbp 150 10 a a DT cord-328082-7c7slfbp 150 11 caffeine caffeine NN cord-328082-7c7slfbp 150 12 - - HYPH cord-328082-7c7slfbp 150 13 free free JJ cord-328082-7c7slfbp 150 14 medium medium NN cord-328082-7c7slfbp 150 15 at at IN cord-328082-7c7slfbp 150 16 20~ 20~ CD cord-328082-7c7slfbp 151 1 ( ( -LRB- cord-328082-7c7slfbp 151 2 Fig Fig NNP cord-328082-7c7slfbp 151 3 . . . cord-328082-7c7slfbp 152 1 3 3 CD cord-328082-7c7slfbp 152 2 c c NN cord-328082-7c7slfbp 152 3 ) ) -RRB- cord-328082-7c7slfbp 152 4 , , , cord-328082-7c7slfbp 152 5 man man NN cord-328082-7c7slfbp 152 6 II II NNP cord-328082-7c7slfbp 152 7 appears appear VBZ cord-328082-7c7slfbp 152 8 in in IN cord-328082-7c7slfbp 152 9 peripheral peripheral JJ cord-328082-7c7slfbp 152 10 and and CC cord-328082-7c7slfbp 152 11 more more JJR cord-328082-7c7slfbp 152 12 central central JJ cord-328082-7c7slfbp 152 13 elements element NNS cord-328082-7c7slfbp 152 14 ( ( -LRB- cord-328082-7c7slfbp 152 15 in in IN cord-328082-7c7slfbp 152 16 47 47 CD cord-328082-7c7slfbp 152 17 % % NN cord-328082-7c7slfbp 152 18 of of IN cord-328082-7c7slfbp 152 19 the the DT cord-328082-7c7slfbp 152 20 cells cell NNS cord-328082-7c7slfbp 152 21 ) ) -RRB- cord-328082-7c7slfbp 152 22 , , , cord-328082-7c7slfbp 152 23 and and CC cord-328082-7c7slfbp 152 24 also also RB cord-328082-7c7slfbp 152 25 in in IN cord-328082-7c7slfbp 152 26 the the DT cord-328082-7c7slfbp 152 27 ER ER NNP cord-328082-7c7slfbp 152 28 . . . cord-328082-7c7slfbp 153 1 After after IN cord-328082-7c7slfbp 153 2 a a DT cord-328082-7c7slfbp 153 3 120-min 120-min CD cord-328082-7c7slfbp 153 4 chase chase NN cord-328082-7c7slfbp 153 5 in in IN cord-328082-7c7slfbp 153 6 caffeine caffeine NN cord-328082-7c7slfbp 153 7 - - HYPH cord-328082-7c7slfbp 153 8 free free JJ cord-328082-7c7slfbp 153 9 medium medium NN cord-328082-7c7slfbp 153 10 at at IN cord-328082-7c7slfbp 153 11 20~ 20~ CD cord-328082-7c7slfbp 154 1 the the DT cord-328082-7c7slfbp 154 2 ER ER NNP cord-328082-7c7slfbp 154 3 was be VBD cord-328082-7c7slfbp 154 4 efficiently efficiently RB cord-328082-7c7slfbp 154 5 emptied empty VBN cord-328082-7c7slfbp 154 6 and and CC cord-328082-7c7slfbp 154 7 man man NN cord-328082-7c7slfbp 154 8 II II NNP cord-328082-7c7slfbp 154 9 was be VBD cord-328082-7c7slfbp 154 10 found find VBN cord-328082-7c7slfbp 154 11 in in IN cord-328082-7c7slfbp 154 12 a a DT cord-328082-7c7slfbp 154 13 few few JJ cord-328082-7c7slfbp 154 14 peripheral peripheral JJ cord-328082-7c7slfbp 154 15 elements element NNS cord-328082-7c7slfbp 154 16 , , , cord-328082-7c7slfbp 154 17 but but CC cord-328082-7c7slfbp 154 18 was be VBD cord-328082-7c7slfbp 154 19 mainly mainly RB cord-328082-7c7slfbp 154 20 perinuclearly perinuclearly RB cord-328082-7c7slfbp 154 21 located locate VBN cord-328082-7c7slfbp 154 22 ( ( -LRB- cord-328082-7c7slfbp 154 23 Fig Fig NNP cord-328082-7c7slfbp 154 24 . . . cord-328082-7c7slfbp 154 25 3 3 CD cord-328082-7c7slfbp 154 26 e e NN cord-328082-7c7slfbp 154 27 ) ) -RRB- cord-328082-7c7slfbp 155 1 ( ( -LRB- cord-328082-7c7slfbp 155 2 in in IN cord-328082-7c7slfbp 155 3 95 95 CD cord-328082-7c7slfbp 155 4 % % NN cord-328082-7c7slfbp 155 5 of of IN cord-328082-7c7slfbp 155 6 the the DT cord-328082-7c7slfbp 155 7 cells cell NNS cord-328082-7c7slfbp 155 8 ) ) -RRB- cord-328082-7c7slfbp 155 9 . . . cord-328082-7c7slfbp 156 1 After after IN cord-328082-7c7slfbp 156 2 180 180 CD cord-328082-7c7slfbp 156 3 min min NN cord-328082-7c7slfbp 156 4 in in IN cord-328082-7c7slfbp 156 5 caffeine caffeine NN cord-328082-7c7slfbp 156 6 - - HYPH cord-328082-7c7slfbp 156 7 free free JJ cord-328082-7c7slfbp 156 8 medium medium NN cord-328082-7c7slfbp 156 9 , , , cord-328082-7c7slfbp 156 10 man man NN cord-328082-7c7slfbp 156 11 II II NNP cord-328082-7c7slfbp 156 12 was be VBD cord-328082-7c7slfbp 156 13 found find VBN cord-328082-7c7slfbp 156 14 perinuclearly perinuclearly RB cord-328082-7c7slfbp 156 15 located locate VBN cord-328082-7c7slfbp 156 16 with with IN cord-328082-7c7slfbp 156 17 no no DT cord-328082-7c7slfbp 156 18 detectable detectable JJ cord-328082-7c7slfbp 156 19 labeling labeling NN cord-328082-7c7slfbp 156 20 in in IN cord-328082-7c7slfbp 156 21 the the DT cord-328082-7c7slfbp 156 22 ER ER NNP cord-328082-7c7slfbp 156 23 ( ( -LRB- cord-328082-7c7slfbp 156 24 Fig fig NN cord-328082-7c7slfbp 156 25 . . . cord-328082-7c7slfbp 156 26 3 3 CD cord-328082-7c7slfbp 156 27 g g NN cord-328082-7c7slfbp 156 28 ) ) -RRB- cord-328082-7c7slfbp 156 29 ( ( -LRB- cord-328082-7c7slfbp 156 30 in in IN cord-328082-7c7slfbp 156 31 98 98 CD cord-328082-7c7slfbp 156 32 % % NN cord-328082-7c7slfbp 156 33 of of IN cord-328082-7c7slfbp 156 34 the the DT cord-328082-7c7slfbp 156 35 cells cell NNS cord-328082-7c7slfbp 156 36 ) ) -RRB- cord-328082-7c7slfbp 156 37 . . . cord-328082-7c7slfbp 157 1 When when WRB cord-328082-7c7slfbp 157 2 the the DT cord-328082-7c7slfbp 157 3 cells cell NNS cord-328082-7c7slfbp 157 4 were be VBD cord-328082-7c7slfbp 157 5 chased chase VBN cord-328082-7c7slfbp 157 6 after after IN cord-328082-7c7slfbp 157 7 the the DT cord-328082-7c7slfbp 157 8 removal removal NN cord-328082-7c7slfbp 157 9 of of IN cord-328082-7c7slfbp 157 10 the the DT cord-328082-7c7slfbp 157 11 BFA BFA NNP cord-328082-7c7slfbp 157 12 in in IN cord-328082-7c7slfbp 157 13 the the DT cord-328082-7c7slfbp 157 14 presence presence NN cord-328082-7c7slfbp 157 15 of of IN cord-328082-7c7slfbp 157 16 10 10 CD cord-328082-7c7slfbp 157 17 mM mm CD cord-328082-7c7slfbp 157 18 caffeine caffeine NN cord-328082-7c7slfbp 157 19 at at IN cord-328082-7c7slfbp 157 20 20~ 20~ CD cord-328082-7c7slfbp 157 21 for for IN cord-328082-7c7slfbp 157 22 60 60 CD cord-328082-7c7slfbp 157 23 min min NN cord-328082-7c7slfbp 157 24 , , , cord-328082-7c7slfbp 157 25 man man NN cord-328082-7c7slfbp 157 26 II II NNP cord-328082-7c7slfbp 157 27 failed fail VBD cord-328082-7c7slfbp 157 28 to to TO cord-328082-7c7slfbp 157 29 exit exit VB cord-328082-7c7slfbp 157 30 the the DT cord-328082-7c7slfbp 157 31 ER ER NNP cord-328082-7c7slfbp 157 32 ( ( -LRB- cord-328082-7c7slfbp 157 33 Fig fig NN cord-328082-7c7slfbp 157 34 . . . cord-328082-7c7slfbp 157 35 _SP cord-328082-7c7slfbp 158 1 3 3 LS cord-328082-7c7slfbp 158 2 d d NN cord-328082-7c7slfbp 158 3 ) ) -RRB- cord-328082-7c7slfbp 158 4 ( ( -LRB- cord-328082-7c7slfbp 158 5 in in IN cord-328082-7c7slfbp 158 6 98 98 CD cord-328082-7c7slfbp 158 7 % % NN cord-328082-7c7slfbp 158 8 of of IN cord-328082-7c7slfbp 158 9 the the DT cord-328082-7c7slfbp 158 10 cells cell NNS cord-328082-7c7slfbp 158 11 ) ) -RRB- cord-328082-7c7slfbp 158 12 . . . cord-328082-7c7slfbp 159 1 After after IN cord-328082-7c7slfbp 159 2 a a DT cord-328082-7c7slfbp 159 3 120- 120- CD cord-328082-7c7slfbp 159 4 ( ( -LRB- cord-328082-7c7slfbp 159 5 Fig fig NN cord-328082-7c7slfbp 159 6 . . . cord-328082-7c7slfbp 159 7 3 3 CD cord-328082-7c7slfbp 159 8 f f NNP cord-328082-7c7slfbp 159 9 ) ) -RRB- cord-328082-7c7slfbp 159 10 or or CC cord-328082-7c7slfbp 159 11 180-rain 180-rain CD cord-328082-7c7slfbp 159 12 ( ( -LRB- cord-328082-7c7slfbp 159 13 Fig Fig NNP cord-328082-7c7slfbp 159 14 . . . cord-328082-7c7slfbp 159 15 3 3 CD cord-328082-7c7slfbp 159 16 h h NN cord-328082-7c7slfbp 159 17 ) ) -RRB- cord-328082-7c7slfbp 159 18 chase chase NN cord-328082-7c7slfbp 159 19 in in IN cord-328082-7c7slfbp 159 20 the the DT cord-328082-7c7slfbp 159 21 presence presence NN cord-328082-7c7slfbp 159 22 of of IN cord-328082-7c7slfbp 159 23 caffeine caffeine NN cord-328082-7c7slfbp 159 24 , , , cord-328082-7c7slfbp 159 25 man man NN cord-328082-7c7slfbp 159 26 II II NNP cord-328082-7c7slfbp 159 27 was be VBD cord-328082-7c7slfbp 159 28 still still RB cord-328082-7c7slfbp 159 29 located locate VBN cord-328082-7c7slfbp 159 30 in in IN cord-328082-7c7slfbp 159 31 the the DT cord-328082-7c7slfbp 159 32 ER ER NNP cord-328082-7c7slfbp 159 33 ( ( -LRB- cord-328082-7c7slfbp 159 34 in in IN cord-328082-7c7slfbp 159 35 94 94 CD cord-328082-7c7slfbp 159 36 and and CC cord-328082-7c7slfbp 159 37 96 96 CD cord-328082-7c7slfbp 159 38 % % NN cord-328082-7c7slfbp 159 39 of of IN cord-328082-7c7slfbp 159 40 the the DT cord-328082-7c7slfbp 159 41 ceils ceil NNS cord-328082-7c7slfbp 159 42 , , , cord-328082-7c7slfbp 159 43 respectively respectively RB cord-328082-7c7slfbp 159 44 ) ) -RRB- cord-328082-7c7slfbp 159 45 . . . cord-328082-7c7slfbp 160 1 These these DT cord-328082-7c7slfbp 160 2 results result NNS cord-328082-7c7slfbp 160 3 show show VBP cord-328082-7c7slfbp 160 4 that that IN cord-328082-7c7slfbp 160 5 10 10 CD cord-328082-7c7slfbp 160 6 mM mm CD cord-328082-7c7slfbp 160 7 caffeine caffeine NN cord-328082-7c7slfbp 160 8 and and CC cord-328082-7c7slfbp 160 9 20~ 20~ CD cord-328082-7c7slfbp 160 10 inhibited inhibit VBD cord-328082-7c7slfbp 160 11 efficiently efficiently RB cord-328082-7c7slfbp 160 12 the the DT cord-328082-7c7slfbp 160 13 movement movement NN cord-328082-7c7slfbp 160 14 of of IN cord-328082-7c7slfbp 160 15 man man NNP cord-328082-7c7slfbp 160 16 II II NNP cord-328082-7c7slfbp 160 17 , , , cord-328082-7c7slfbp 160 18 a a DT cord-328082-7c7slfbp 160 19 resident resident JJ cord-328082-7c7slfbp 160 20 Golgi Golgi NNP cord-328082-7c7slfbp 160 21 membrane membrane NN cord-328082-7c7slfbp 160 22 protein protein NN cord-328082-7c7slfbp 160 23 , , , cord-328082-7c7slfbp 160 24 out out RB cord-328082-7c7slfbp 160 25 from from IN cord-328082-7c7slfbp 160 26 the the DT cord-328082-7c7slfbp 160 27 ER ER NNP cord-328082-7c7slfbp 160 28 . . . cord-328082-7c7slfbp 161 1 This this DT cord-328082-7c7slfbp 161 2 may may MD cord-328082-7c7slfbp 161 3 further further RB cord-328082-7c7slfbp 161 4 suggest suggest VB cord-328082-7c7slfbp 161 5 that that IN cord-328082-7c7slfbp 161 6 caffeine caffeine NN cord-328082-7c7slfbp 161 7 at at IN cord-328082-7c7slfbp 161 8 reduced reduce VBN cord-328082-7c7slfbp 161 9 temperature temperature NN cord-328082-7c7slfbp 161 10 inhibits inhibit VBZ cord-328082-7c7slfbp 161 11 the the DT cord-328082-7c7slfbp 161 12 recovery recovery NN cord-328082-7c7slfbp 161 13 of of IN cord-328082-7c7slfbp 161 14 the the DT cord-328082-7c7slfbp 161 15 Golgi Golgi NNP cord-328082-7c7slfbp 161 16 from from IN cord-328082-7c7slfbp 161 17 the the DT cord-328082-7c7slfbp 161 18 BFA BFA NNP cord-328082-7c7slfbp 161 19 treatment treatment NN cord-328082-7c7slfbp 161 20 and and CC cord-328082-7c7slfbp 161 21 thus thus RB cord-328082-7c7slfbp 161 22 inhibits inhibit VBZ cord-328082-7c7slfbp 161 23 the the DT cord-328082-7c7slfbp 161 24 membrane membrane NN cord-328082-7c7slfbp 161 25 traffic traffic NN cord-328082-7c7slfbp 161 26 from from IN cord-328082-7c7slfbp 161 27 the the DT cord-328082-7c7slfbp 161 28 ER ER NNP cord-328082-7c7slfbp 161 29 to to IN cord-328082-7c7slfbp 161 30 the the DT cord-328082-7c7slfbp 161 31 Golgi Golgi NNP cord-328082-7c7slfbp 161 32 complex complex NN cord-328082-7c7slfbp 161 33 , , , cord-328082-7c7slfbp 161 34 Figure figure NN cord-328082-7c7slfbp 161 35 3 3 CD cord-328082-7c7slfbp 161 36 . . . cord-328082-7c7slfbp 162 1 10 10 CD cord-328082-7c7slfbp 162 2 mM mm CD cord-328082-7c7slfbp 162 3 caffeine caffeine NN cord-328082-7c7slfbp 162 4 at at IN cord-328082-7c7slfbp 162 5 20~ 20~ CD cord-328082-7c7slfbp 162 6 inhibits inhibit VBZ cord-328082-7c7slfbp 162 7 the the DT cord-328082-7c7slfbp 162 8 movement movement NN cord-328082-7c7slfbp 162 9 of of IN cord-328082-7c7slfbp 162 10 man man NNP cord-328082-7c7slfbp 162 11 II II NNP cord-328082-7c7slfbp 162 12 out out RP cord-328082-7c7slfbp 162 13 from from IN cord-328082-7c7slfbp 162 14 the the DT cord-328082-7c7slfbp 162 15 ER ER NNP cord-328082-7c7slfbp 162 16 . . . cord-328082-7c7slfbp 163 1 a a DT cord-328082-7c7slfbp 163 2 shows show VBZ cord-328082-7c7slfbp 163 3 the the DT cord-328082-7c7slfbp 163 4 normal normal JJ cord-328082-7c7slfbp 163 5 distribution distribution NN cord-328082-7c7slfbp 163 6 of of IN cord-328082-7c7slfbp 163 7 man man NN cord-328082-7c7slfbp 163 8 II II NNP cord-328082-7c7slfbp 163 9 in in IN cord-328082-7c7slfbp 163 10 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 163 11 cells cell NNS cord-328082-7c7slfbp 163 12 . . . cord-328082-7c7slfbp 164 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 165 1 cells cell NNS cord-328082-7c7slfbp 165 2 were be VBD cord-328082-7c7slfbp 165 3 treated treat VBN cord-328082-7c7slfbp 165 4 with with IN cord-328082-7c7slfbp 165 5 BFA BFA NNP cord-328082-7c7slfbp 165 6 ( ( -LRB- cord-328082-7c7slfbp 165 7 2 2 CD cord-328082-7c7slfbp 165 8 # # NN cord-328082-7c7slfbp 165 9 g g NN cord-328082-7c7slfbp 165 10 / / SYM cord-328082-7c7slfbp 165 11 ml ml NN cord-328082-7c7slfbp 165 12 ) ) -RRB- cord-328082-7c7slfbp 165 13 at at IN cord-328082-7c7slfbp 165 14 37~ 37~ CD cord-328082-7c7slfbp 165 15 for for IN cord-328082-7c7slfbp 165 16 90 90 CD cord-328082-7c7slfbp 165 17 rain rain NN cord-328082-7c7slfbp 165 18 ( ( -LRB- cord-328082-7c7slfbp 165 19 b b NN cord-328082-7c7slfbp 165 20 ) ) -RRB- cord-328082-7c7slfbp 165 21 or or CC cord-328082-7c7slfbp 165 22 treated treat VBN cord-328082-7c7slfbp 165 23 with with IN cord-328082-7c7slfbp 165 24 BFA BFA NNP cord-328082-7c7slfbp 165 25 for for IN cord-328082-7c7slfbp 165 26 90 90 CD cord-328082-7c7slfbp 165 27 min min NN cord-328082-7c7slfbp 165 28 followed follow VBN cord-328082-7c7slfbp 165 29 by by IN cord-328082-7c7slfbp 165 30 washout washout NN cord-328082-7c7slfbp 165 31 with with IN cord-328082-7c7slfbp 165 32 a a DT cord-328082-7c7slfbp 165 33 medium medium NN cord-328082-7c7slfbp 165 34 supplcmented supplcmente VBN cord-328082-7c7slfbp 165 35 with with IN cord-328082-7c7slfbp 165 36 or or CC cord-328082-7c7slfbp 165 37 without without IN cord-328082-7c7slfbp 165 38 10 10 CD cord-328082-7c7slfbp 165 39 mM mm CD cord-328082-7c7slfbp 165 40 caffeine caffeine NN cord-328082-7c7slfbp 165 41 at at IN cord-328082-7c7slfbp 165 42 20~ 20~ CD cord-328082-7c7slfbp 166 1 The the DT cord-328082-7c7slfbp 166 2 cells cell NNS cord-328082-7c7slfbp 166 3 were be VBD cord-328082-7c7slfbp 166 4 then then RB cord-328082-7c7slfbp 166 5 chased chase VBN cord-328082-7c7slfbp 166 6 for for IN cord-328082-7c7slfbp 166 7 60 60 CD cord-328082-7c7slfbp 166 8 , , , cord-328082-7c7slfbp 166 9 120 120 CD cord-328082-7c7slfbp 166 10 , , , cord-328082-7c7slfbp 166 11 or or CC cord-328082-7c7slfbp 166 12 180 180 CD cord-328082-7c7slfbp 166 13 rain rain NN cord-328082-7c7slfbp 166 14 at at IN cord-328082-7c7slfbp 166 15 20~ 20~ CD cord-328082-7c7slfbp 166 16 in in IN cord-328082-7c7slfbp 166 17 the the DT cord-328082-7c7slfbp 166 18 absence absence NN cord-328082-7c7slfbp 166 19 ( ( -LRB- cord-328082-7c7slfbp 166 20 c c NN cord-328082-7c7slfbp 166 21 , , , cord-328082-7c7slfbp 166 22 e e NNP cord-328082-7c7slfbp 166 23 , , , cord-328082-7c7slfbp 166 24 and and CC cord-328082-7c7slfbp 166 25 g g NN cord-328082-7c7slfbp 166 26 , , , cord-328082-7c7slfbp 166 27 respectively respectively RB cord-328082-7c7slfbp 166 28 ) ) -RRB- cord-328082-7c7slfbp 166 29 or or CC cord-328082-7c7slfbp 166 30 presence presence NN cord-328082-7c7slfbp 166 31 of of IN cord-328082-7c7slfbp 166 32 10 10 CD cord-328082-7c7slfbp 166 33 mM mm CD cord-328082-7c7slfbp 166 34 caffeine caffeine NN cord-328082-7c7slfbp 166 35 ( ( -LRB- cord-328082-7c7slfbp 166 36 d d NNP cord-328082-7c7slfbp 166 37 , , , cord-328082-7c7slfbp 166 38 f f NNP cord-328082-7c7slfbp 166 39 , , , cord-328082-7c7slfbp 166 40 and and CC cord-328082-7c7slfbp 166 41 h h NNP cord-328082-7c7slfbp 166 42 , , , cord-328082-7c7slfbp 166 43 respectively respectively RB cord-328082-7c7slfbp 166 44 ) ) -RRB- cord-328082-7c7slfbp 166 45 , , , cord-328082-7c7slfbp 166 46 fixed fix VBN cord-328082-7c7slfbp 166 47 , , , cord-328082-7c7slfbp 166 48 and and CC cord-328082-7c7slfbp 166 49 labeled label VBN cord-328082-7c7slfbp 166 50 with with IN cord-328082-7c7slfbp 166 51 antibodies antibody NNS cord-328082-7c7slfbp 166 52 to to TO cord-328082-7c7slfbp 166 53 man man VB cord-328082-7c7slfbp 166 54 II II NNP cord-328082-7c7slfbp 166 55 . . . cord-328082-7c7slfbp 167 1 Incubation incubation NN cord-328082-7c7slfbp 167 2 of of IN cord-328082-7c7slfbp 167 3 the the DT cord-328082-7c7slfbp 167 4 cells cell NNS cord-328082-7c7slfbp 167 5 in in IN cord-328082-7c7slfbp 167 6 the the DT cord-328082-7c7slfbp 167 7 presence presence NN cord-328082-7c7slfbp 167 8 of of IN cord-328082-7c7slfbp 167 9 caffeine caffeine NN cord-328082-7c7slfbp 167 10 at at IN cord-328082-7c7slfbp 167 11 20~ 20~ CD cord-328082-7c7slfbp 167 12 inhibits inhibit VBZ cord-328082-7c7slfbp 167 13 the the DT cord-328082-7c7slfbp 167 14 exit exit NN cord-328082-7c7slfbp 167 15 of of IN cord-328082-7c7slfbp 167 16 man man NN cord-328082-7c7slfbp 167 17 II II NNP cord-328082-7c7slfbp 167 18 from from IN cord-328082-7c7slfbp 167 19 th th XX cord-328082-7c7slfbp 167 20 ER er NN cord-328082-7c7slfbp 167 21 after after IN cord-328082-7c7slfbp 167 22 the the DT cord-328082-7c7slfbp 167 23 removal removal NN cord-328082-7c7slfbp 167 24 of of IN cord-328082-7c7slfbp 167 25 the the DT cord-328082-7c7slfbp 167 26 BFA BFA NNP cord-328082-7c7slfbp 167 27 ( ( -LRB- cord-328082-7c7slfbp 167 28 d d NNP cord-328082-7c7slfbp 167 29 , , , cord-328082-7c7slfbp 167 30 f f NNP cord-328082-7c7slfbp 167 31 , , , cord-328082-7c7slfbp 167 32 and and CC cord-328082-7c7slfbp 167 33 h h NNP cord-328082-7c7slfbp 167 34 ) ) -RRB- cord-328082-7c7slfbp 167 35 . . . cord-328082-7c7slfbp 168 1 In in IN cord-328082-7c7slfbp 168 2 control control NN cord-328082-7c7slfbp 168 3 cells cell NNS cord-328082-7c7slfbp 168 4 at at IN cord-328082-7c7slfbp 168 5 20~ 20~ CD cord-328082-7c7slfbp 168 6 already already RB cord-328082-7c7slfbp 168 7 60 60 CD cord-328082-7c7slfbp 168 8 rain rain NN cord-328082-7c7slfbp 168 9 after after IN cord-328082-7c7slfbp 168 10 the the DT cord-328082-7c7slfbp 168 11 BFA BFA NNP cord-328082-7c7slfbp 168 12 removal removal NN cord-328082-7c7slfbp 168 13 , , , cord-328082-7c7slfbp 168 14 man man NN cord-328082-7c7slfbp 168 15 II II NNP cord-328082-7c7slfbp 168 16 can can MD cord-328082-7c7slfbp 168 17 be be VB cord-328082-7c7slfbp 168 18 seen see VBN cord-328082-7c7slfbp 168 19 to to TO cord-328082-7c7slfbp 168 20 be be VB cord-328082-7c7slfbp 168 21 located locate VBN cord-328082-7c7slfbp 168 22 in in IN cord-328082-7c7slfbp 168 23 peripheral peripheral JJ cord-328082-7c7slfbp 168 24 elements element NNS cord-328082-7c7slfbp 168 25 and and CC cord-328082-7c7slfbp 168 26 in in IN cord-328082-7c7slfbp 168 27 some some DT cord-328082-7c7slfbp 168 28 degree degree NN cord-328082-7c7slfbp 168 29 in in IN cord-328082-7c7slfbp 168 30 the the DT cord-328082-7c7slfbp 168 31 ER ER NNP cord-328082-7c7slfbp 168 32 ( ( -LRB- cord-328082-7c7slfbp 168 33 c c NN cord-328082-7c7slfbp 168 34 ) ) -RRB- cord-328082-7c7slfbp 168 35 . . . cord-328082-7c7slfbp 169 1 After after IN cord-328082-7c7slfbp 169 2 120 120 CD cord-328082-7c7slfbp 169 3 min min NN cord-328082-7c7slfbp 169 4 , , , cord-328082-7c7slfbp 169 5 man man NN cord-328082-7c7slfbp 169 6 II II NNP cord-328082-7c7slfbp 169 7 is be VBZ cord-328082-7c7slfbp 169 8 mainly mainly RB cord-328082-7c7slfbp 169 9 in in IN cord-328082-7c7slfbp 169 10 perinuclear perinuclear NN cord-328082-7c7slfbp 169 11 elements element NNS cord-328082-7c7slfbp 169 12 ( ( -LRB- cord-328082-7c7slfbp 169 13 e e NN cord-328082-7c7slfbp 169 14 ) ) -RRB- cord-328082-7c7slfbp 169 15 . . . cord-328082-7c7slfbp 170 1 After after IN cord-328082-7c7slfbp 170 2 a a DT cord-328082-7c7slfbp 170 3 180-rain 180-rain CD cord-328082-7c7slfbp 170 4 chase chase NN cord-328082-7c7slfbp 170 5 in in IN cord-328082-7c7slfbp 170 6 a a DT cord-328082-7c7slfbp 170 7 caffeine caffeine NN cord-328082-7c7slfbp 170 8 - - HYPH cord-328082-7c7slfbp 170 9 free free JJ cord-328082-7c7slfbp 170 10 medium medium NN cord-328082-7c7slfbp 170 11 at at IN cord-328082-7c7slfbp 170 12 20~ 20~ CD cord-328082-7c7slfbp 170 13 man man NN cord-328082-7c7slfbp 170 14 II II NNP cord-328082-7c7slfbp 170 15 is be VBZ cord-328082-7c7slfbp 170 16 located locate VBN cord-328082-7c7slfbp 170 17 perinuclearly perinuclearly NNP cord-328082-7c7slfbp 170 18 ( ( -LRB- cord-328082-7c7slfbp 170 19 g g NN cord-328082-7c7slfbp 170 20 ) ) -RRB- cord-328082-7c7slfbp 170 21 . . . cord-328082-7c7slfbp 171 1 Bar bar NN cord-328082-7c7slfbp 171 2 , , , cord-328082-7c7slfbp 171 3 20 20 CD cord-328082-7c7slfbp 171 4 # # NN cord-328082-7c7slfbp 171 5 m. m. NN cord-328082-7c7slfbp 171 6 Since since IN cord-328082-7c7slfbp 171 7 caffeine caffeine NN cord-328082-7c7slfbp 171 8 at at IN cord-328082-7c7slfbp 171 9 20~ 20~ CD cord-328082-7c7slfbp 171 10 inhibited inhibit VBD cord-328082-7c7slfbp 171 11 the the DT cord-328082-7c7slfbp 171 12 ER er NN cord-328082-7c7slfbp 171 13 - - HYPH cord-328082-7c7slfbp 171 14 to to IN cord-328082-7c7slfbp 171 15 - - HYPH cord-328082-7c7slfbp 171 16 Golgi golgi NN cord-328082-7c7slfbp 171 17 membrane membrane NN cord-328082-7c7slfbp 171 18 traffic traffic NN cord-328082-7c7slfbp 171 19 , , , cord-328082-7c7slfbp 171 20 it -PRON- PRP cord-328082-7c7slfbp 171 21 was be VBD cord-328082-7c7slfbp 171 22 of of IN cord-328082-7c7slfbp 171 23 great great JJ cord-328082-7c7slfbp 171 24 interest interest NN cord-328082-7c7slfbp 171 25 to to TO cord-328082-7c7slfbp 171 26 study study VB cord-328082-7c7slfbp 171 27 whether whether IN cord-328082-7c7slfbp 171 28 10 10 CD cord-328082-7c7slfbp 171 29 mM mm CD cord-328082-7c7slfbp 171 30 caffeine caffeine NN cord-328082-7c7slfbp 171 31 would would MD cord-328082-7c7slfbp 171 32 also also RB cord-328082-7c7slfbp 171 33 inhibit inhibit VB cord-328082-7c7slfbp 171 34 the the DT cord-328082-7c7slfbp 171 35 Golgi Golgi NNP cord-328082-7c7slfbp 171 36 - - HYPH cord-328082-7c7slfbp 171 37 to to IN cord-328082-7c7slfbp 171 38 - - HYPH cord-328082-7c7slfbp 171 39 ER er NN cord-328082-7c7slfbp 171 40 traffic traffic NN cord-328082-7c7slfbp 171 41 . . . cord-328082-7c7slfbp 172 1 Recently recently RB cord-328082-7c7slfbp 172 2 , , , cord-328082-7c7slfbp 172 3 the the DT cord-328082-7c7slfbp 172 4 fungal fungal JJ cord-328082-7c7slfbp 172 5 antibiotic antibiotic NN cord-328082-7c7slfbp 172 6 BFA BFA NNP cord-328082-7c7slfbp 172 7 has have VBZ cord-328082-7c7slfbp 172 8 been be VBN cord-328082-7c7slfbp 172 9 used use VBN cord-328082-7c7slfbp 172 10 to to TO cord-328082-7c7slfbp 172 11 study study VB cord-328082-7c7slfbp 172 12 the the DT cord-328082-7c7slfbp 172 13 retrograde retrograde JJ cord-328082-7c7slfbp 172 14 transport transport NN cord-328082-7c7slfbp 172 15 mechanism mechanism NN cord-328082-7c7slfbp 172 16 from from IN cord-328082-7c7slfbp 172 17 the the DT cord-328082-7c7slfbp 172 18 Golgi Golgi NNP cord-328082-7c7slfbp 172 19 complex complex NN cord-328082-7c7slfbp 172 20 to to IN cord-328082-7c7slfbp 172 21 the the DT cord-328082-7c7slfbp 172 22 ER er NN cord-328082-7c7slfbp 172 23 ( ( -LRB- cord-328082-7c7slfbp 172 24 Doms Doms NNP cord-328082-7c7slfbp 172 25 et et FW cord-328082-7c7slfbp 172 26 al al NNP cord-328082-7c7slfbp 172 27 . . . cord-328082-7c7slfbp 173 1 , , , cord-328082-7c7slfbp 173 2 1989 1989 CD cord-328082-7c7slfbp 173 3 ; ; : cord-328082-7c7slfbp 173 4 Lippincott Lippincott NNP cord-328082-7c7slfbp 173 5 - - HYPH cord-328082-7c7slfbp 173 6 Schwartz Schwartz NNP cord-328082-7c7slfbp 173 7 et et NNP cord-328082-7c7slfbp 173 8 al al NNP cord-328082-7c7slfbp 173 9 . . NNP cord-328082-7c7slfbp 173 10 , , , cord-328082-7c7slfbp 173 11 1989 1989 CD cord-328082-7c7slfbp 173 12 , , , cord-328082-7c7slfbp 173 13 1991a 1991a CD cord-328082-7c7slfbp 173 14 Fujiwara Fujiwara NNP cord-328082-7c7slfbp 173 15 et et NNP cord-328082-7c7slfbp 173 16 al al NNP cord-328082-7c7slfbp 173 17 . . NNP cord-328082-7c7slfbp 173 18 , , , cord-328082-7c7slfbp 173 19 1988 1988 CD cord-328082-7c7slfbp 173 20 ) ) -RRB- cord-328082-7c7slfbp 173 21 . . . cord-328082-7c7slfbp 174 1 We -PRON- PRP cord-328082-7c7slfbp 174 2 therefore therefore RB cord-328082-7c7slfbp 174 3 studied study VBD cord-328082-7c7slfbp 174 4 the the DT cord-328082-7c7slfbp 174 5 effect effect NN cord-328082-7c7slfbp 174 6 of of IN cord-328082-7c7slfbp 174 7 10 10 CD cord-328082-7c7slfbp 174 8 m m NN cord-328082-7c7slfbp 174 9 M M NNP cord-328082-7c7slfbp 174 10 caffeine caffeine NN cord-328082-7c7slfbp 174 11 on on IN cord-328082-7c7slfbp 174 12 the the DT cord-328082-7c7slfbp 174 13 BFA BFA NNP cord-328082-7c7slfbp 174 14 - - HYPH cord-328082-7c7slfbp 174 15 induced induce VBN cord-328082-7c7slfbp 174 16 redistribution redistribution NN cord-328082-7c7slfbp 174 17 of of IN cord-328082-7c7slfbp 174 18 Golgi Golgi NNP cord-328082-7c7slfbp 174 19 membranes membrane NNS cord-328082-7c7slfbp 174 20 at at IN cord-328082-7c7slfbp 174 21 20~ 20~ CD cord-328082-7c7slfbp 174 22 using use VBG cord-328082-7c7slfbp 174 23 man man NN cord-328082-7c7slfbp 174 24 II II NNP cord-328082-7c7slfbp 174 25 as as IN cord-328082-7c7slfbp 174 26 a a DT cord-328082-7c7slfbp 174 27 marker marker NN cord-328082-7c7slfbp 174 28 . . . cord-328082-7c7slfbp 175 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 176 1 cells cell NNS cord-328082-7c7slfbp 176 2 were be VBD cord-328082-7c7slfbp 176 3 treated treat VBN cord-328082-7c7slfbp 176 4 for for IN cord-328082-7c7slfbp 176 5 60 60 CD cord-328082-7c7slfbp 176 6 , , , cord-328082-7c7slfbp 176 7 120 120 CD cord-328082-7c7slfbp 176 8 , , , cord-328082-7c7slfbp 176 9 or or CC cord-328082-7c7slfbp 176 10 180 180 CD cord-328082-7c7slfbp 176 11 min min NN cord-328082-7c7slfbp 176 12 with with IN cord-328082-7c7slfbp 176 13 5 5 CD cord-328082-7c7slfbp 176 14 # # $ cord-328082-7c7slfbp 176 15 g g NN cord-328082-7c7slfbp 176 16 / / SYM cord-328082-7c7slfbp 176 17 ml ml NN cord-328082-7c7slfbp 176 18 of of IN cord-328082-7c7slfbp 176 19 BFA BFA NNP cord-328082-7c7slfbp 176 20 and and CC cord-328082-7c7slfbp 176 21 50 50 CD cord-328082-7c7slfbp 176 22 # # NN cord-328082-7c7slfbp 176 23 g g NN cord-328082-7c7slfbp 176 24 / / SYM cord-328082-7c7slfbp 176 25 ml ml NN cord-328082-7c7slfbp 176 26 of of IN cord-328082-7c7slfbp 176 27 cycloheximide cycloheximide NN cord-328082-7c7slfbp 176 28 at at IN cord-328082-7c7slfbp 176 29 20~ 20~ CD cord-328082-7c7slfbp 176 30 in in IN cord-328082-7c7slfbp 176 31 the the DT cord-328082-7c7slfbp 176 32 presence presence NN cord-328082-7c7slfbp 176 33 or or CC cord-328082-7c7slfbp 176 34 absence absence NN cord-328082-7c7slfbp 176 35 of of IN cord-328082-7c7slfbp 176 36 10 10 CD cord-328082-7c7slfbp 176 37 m m NN cord-328082-7c7slfbp 176 38 M M NNP cord-328082-7c7slfbp 176 39 caffeine caffeine NN cord-328082-7c7slfbp 176 40 . . . cord-328082-7c7slfbp 177 1 In in IN cord-328082-7c7slfbp 177 2 Fig Fig NNP cord-328082-7c7slfbp 177 3 . . . cord-328082-7c7slfbp 178 1 4 4 LS cord-328082-7c7slfbp 178 2 a a DT cord-328082-7c7slfbp 178 3 the the DT cord-328082-7c7slfbp 178 4 distribution distribution NN cord-328082-7c7slfbp 178 5 of of IN cord-328082-7c7slfbp 178 6 the the DT cord-328082-7c7slfbp 178 7 Golgi Golgi NNP cord-328082-7c7slfbp 178 8 marker marker NN cord-328082-7c7slfbp 178 9 man man NN cord-328082-7c7slfbp 178 10 II II NNP cord-328082-7c7slfbp 178 11 is be VBZ cord-328082-7c7slfbp 178 12 shown show VBN cord-328082-7c7slfbp 178 13 before before IN cord-328082-7c7slfbp 178 14 any any DT cord-328082-7c7slfbp 178 15 drug drug NN cord-328082-7c7slfbp 178 16 treatments treatment NNS cord-328082-7c7slfbp 178 17 . . . cord-328082-7c7slfbp 179 1 When when WRB cord-328082-7c7slfbp 179 2 the the DT cord-328082-7c7slfbp 179 3 cells cell NNS cord-328082-7c7slfbp 179 4 were be VBD cord-328082-7c7slfbp 179 5 treated treat VBN cord-328082-7c7slfbp 179 6 with with IN cord-328082-7c7slfbp 179 7 5 5 CD cord-328082-7c7slfbp 179 8 / / SYM cord-328082-7c7slfbp 179 9 zg zg NNP cord-328082-7c7slfbp 179 10 / / , cord-328082-7c7slfbp 179 11 ml ml NN cord-328082-7c7slfbp 179 12 of of IN cord-328082-7c7slfbp 179 13 BFA BFA NNP cord-328082-7c7slfbp 179 14 for for IN cord-328082-7c7slfbp 179 15 90 90 CD cord-328082-7c7slfbp 179 16 min min NN cord-328082-7c7slfbp 179 17 at at IN cord-328082-7c7slfbp 179 18 37~ 37~ CD cord-328082-7c7slfbp 179 19 a a DT cord-328082-7c7slfbp 179 20 characteristic characteristic JJ cord-328082-7c7slfbp 179 21 ER er NN cord-328082-7c7slfbp 179 22 staining staining NN cord-328082-7c7slfbp 179 23 pattern pattern NN cord-328082-7c7slfbp 179 24 was be VBD cord-328082-7c7slfbp 179 25 observed observe VBN cord-328082-7c7slfbp 179 26 ( ( -LRB- cord-328082-7c7slfbp 179 27 Fig Fig NNP cord-328082-7c7slfbp 179 28 . . . cord-328082-7c7slfbp 179 29 4 4 CD cord-328082-7c7slfbp 179 30 b b LS cord-328082-7c7slfbp 179 31 ) ) -RRB- cord-328082-7c7slfbp 179 32 . . . cord-328082-7c7slfbp 180 1 When when WRB cord-328082-7c7slfbp 180 2 the the DT cord-328082-7c7slfbp 180 3 cells cell NNS cord-328082-7c7slfbp 180 4 were be VBD cord-328082-7c7slfbp 180 5 treated treat VBN cord-328082-7c7slfbp 180 6 with with IN cord-328082-7c7slfbp 180 7 5 5 CD cord-328082-7c7slfbp 180 8 # # $ cord-328082-7c7slfbp 180 9 g/ g/ NNS cord-328082-7c7slfbp 180 10 ml ml NNS cord-328082-7c7slfbp 180 11 of of IN cord-328082-7c7slfbp 180 12 BFA BFA NNP cord-328082-7c7slfbp 180 13 for for IN cord-328082-7c7slfbp 180 14 60 60 CD cord-328082-7c7slfbp 180 15 rain rain NN cord-328082-7c7slfbp 180 16 at at IN cord-328082-7c7slfbp 180 17 20~ 20~ CD cord-328082-7c7slfbp 181 1 ( ( -LRB- cord-328082-7c7slfbp 181 2 Fig Fig NNP cord-328082-7c7slfbp 181 3 . . . cord-328082-7c7slfbp 182 1 4 4 LS cord-328082-7c7slfbp 182 2 c c LS cord-328082-7c7slfbp 182 3 ) ) -RRB- cord-328082-7c7slfbp 182 4 in in IN cord-328082-7c7slfbp 182 5 60 60 CD cord-328082-7c7slfbp 182 6 % % NN cord-328082-7c7slfbp 182 7 of of IN cord-328082-7c7slfbp 182 8 the the DT cord-328082-7c7slfbp 182 9 cells cell NNS cord-328082-7c7slfbp 182 10 man man NN cord-328082-7c7slfbp 182 11 II II NNP cord-328082-7c7slfbp 182 12 was be VBD cord-328082-7c7slfbp 182 13 found find VBN cord-328082-7c7slfbp 182 14 in in IN cord-328082-7c7slfbp 182 15 a a DT cord-328082-7c7slfbp 182 16 perinuclear perinuclear JJ cord-328082-7c7slfbp 182 17 localization localization NN cord-328082-7c7slfbp 182 18 , , , cord-328082-7c7slfbp 182 19 and and CC cord-328082-7c7slfbp 182 20 in in IN cord-328082-7c7slfbp 182 21 89 89 CD cord-328082-7c7slfbp 182 22 % % NN cord-328082-7c7slfbp 182 23 of of IN cord-328082-7c7slfbp 182 24 the the DT cord-328082-7c7slfbp 182 25 cells cell NNS cord-328082-7c7slfbp 182 26 , , , cord-328082-7c7slfbp 182 27 man man NN cord-328082-7c7slfbp 182 28 II II NNP cord-328082-7c7slfbp 182 29 was be VBD cord-328082-7c7slfbp 182 30 found find VBN cord-328082-7c7slfbp 182 31 to to TO cord-328082-7c7slfbp 182 32 be be VB cord-328082-7c7slfbp 182 33 translocated translocate VBN cord-328082-7c7slfbp 182 34 partly partly RB cord-328082-7c7slfbp 182 35 or or CC cord-328082-7c7slfbp 182 36 completely completely RB cord-328082-7c7slfbp 182 37 to to IN cord-328082-7c7slfbp 182 38 the the DT cord-328082-7c7slfbp 182 39 ER ER NNP cord-328082-7c7slfbp 182 40 . . . cord-328082-7c7slfbp 183 1 After after IN cord-328082-7c7slfbp 183 2 a a DT cord-328082-7c7slfbp 183 3 120-min 120-min CD cord-328082-7c7slfbp 183 4 chase chase NN cord-328082-7c7slfbp 183 5 at at IN cord-328082-7c7slfbp 183 6 20~ 20~ CD cord-328082-7c7slfbp 183 7 in in IN cord-328082-7c7slfbp 183 8 the the DT cord-328082-7c7slfbp 183 9 Figure figure NN cord-328082-7c7slfbp 183 10 4 4 CD cord-328082-7c7slfbp 183 11 . . . cord-328082-7c7slfbp 184 1 The the DT cord-328082-7c7slfbp 184 2 BFA BFA NNP cord-328082-7c7slfbp 184 3 - - HYPH cord-328082-7c7slfbp 184 4 induced induce VBN cord-328082-7c7slfbp 184 5 recycling recycling NN cord-328082-7c7slfbp 184 6 of of IN cord-328082-7c7slfbp 184 7 man man NN cord-328082-7c7slfbp 184 8 II II NNP cord-328082-7c7slfbp 184 9 from from IN cord-328082-7c7slfbp 184 10 the the DT cord-328082-7c7slfbp 184 11 Golgi Golgi NNP cord-328082-7c7slfbp 184 12 complex complex NN cord-328082-7c7slfbp 184 13 to to IN cord-328082-7c7slfbp 184 14 the the DT cord-328082-7c7slfbp 184 15 ER ER NNP cord-328082-7c7slfbp 184 16 is be VBZ cord-328082-7c7slfbp 184 17 not not RB cord-328082-7c7slfbp 184 18 inhibited inhibit VBN cord-328082-7c7slfbp 184 19 by by IN cord-328082-7c7slfbp 184 20 caffeine caffeine NN cord-328082-7c7slfbp 184 21 at at IN cord-328082-7c7slfbp 184 22 20"C. 20"c. CD cord-328082-7c7slfbp 185 1 BHK-21 bhk-21 DT cord-328082-7c7slfbp 185 2 cells cell NNS cord-328082-7c7slfbp 185 3 were be VBD cord-328082-7c7slfbp 185 4 incubated incubate VBN cord-328082-7c7slfbp 185 5 with with IN cord-328082-7c7slfbp 185 6 BFA BFA NNP cord-328082-7c7slfbp 185 7 ( ( -LRB- cord-328082-7c7slfbp 185 8 5 5 CD cord-328082-7c7slfbp 185 9 # # $ cord-328082-7c7slfbp 185 10 g g NN cord-328082-7c7slfbp 185 11 / / SYM cord-328082-7c7slfbp 185 12 ml ml NN cord-328082-7c7slfbp 185 13 ) ) -RRB- cord-328082-7c7slfbp 185 14 at at IN cord-328082-7c7slfbp 185 15 37~ 37~ CD cord-328082-7c7slfbp 185 16 ( ( -LRB- cord-328082-7c7slfbp 185 17 b b NN cord-328082-7c7slfbp 185 18 ) ) -RRB- cord-328082-7c7slfbp 185 19 , , , cord-328082-7c7slfbp 185 20 with with IN cord-328082-7c7slfbp 185 21 BFA BFA NNP cord-328082-7c7slfbp 185 22 ( ( -LRB- cord-328082-7c7slfbp 185 23 5 5 CD cord-328082-7c7slfbp 185 24 ~g ~g NNP cord-328082-7c7slfbp 185 25 / / SYM cord-328082-7c7slfbp 185 26 ml ml NN cord-328082-7c7slfbp 185 27 ) ) -RRB- cord-328082-7c7slfbp 185 28 and and CC cord-328082-7c7slfbp 185 29 10 10 CD cord-328082-7c7slfbp 185 30 mM mm CD cord-328082-7c7slfbp 185 31 caffeine caffeine NN cord-328082-7c7slfbp 185 32 at at IN cord-328082-7c7slfbp 185 33 20~ 20~ CD cord-328082-7c7slfbp 185 34 for for IN cord-328082-7c7slfbp 185 35 60 60 CD cord-328082-7c7slfbp 185 36 , , , cord-328082-7c7slfbp 185 37 120 120 CD cord-328082-7c7slfbp 185 38 , , , cord-328082-7c7slfbp 185 39 and and CC cord-328082-7c7slfbp 185 40 180 180 CD cord-328082-7c7slfbp 185 41 min min NN cord-328082-7c7slfbp 185 42 ( ( -LRB- cord-328082-7c7slfbp 185 43 d d NNP cord-328082-7c7slfbp 185 44 , , , cord-328082-7c7slfbp 185 45 f f NNP cord-328082-7c7slfbp 185 46 , , , cord-328082-7c7slfbp 185 47 and and CC cord-328082-7c7slfbp 185 48 h h NNP cord-328082-7c7slfbp 185 49 , , , cord-328082-7c7slfbp 185 50 respectively respectively RB cord-328082-7c7slfbp 185 51 ) ) -RRB- cord-328082-7c7slfbp 185 52 or or CC cord-328082-7c7slfbp 185 53 with with IN cord-328082-7c7slfbp 185 54 BFA BFA NNP cord-328082-7c7slfbp 185 55 ( ( -LRB- cord-328082-7c7slfbp 185 56 5/~g 5/~g CD cord-328082-7c7slfbp 185 57 / / SYM cord-328082-7c7slfbp 185 58 rnl rnl NNP cord-328082-7c7slfbp 185 59 ) ) -RRB- cord-328082-7c7slfbp 185 60 only only RB cord-328082-7c7slfbp 185 61 at at IN cord-328082-7c7slfbp 185 62 20"C 20"c CD cord-328082-7c7slfbp 185 63 for for IN cord-328082-7c7slfbp 185 64 60 60 CD cord-328082-7c7slfbp 185 65 , , , cord-328082-7c7slfbp 185 66 120 120 CD cord-328082-7c7slfbp 185 67 , , , cord-328082-7c7slfbp 185 68 and and CC cord-328082-7c7slfbp 185 69 180 180 CD cord-328082-7c7slfbp 185 70 min min NN cord-328082-7c7slfbp 185 71 ( ( -LRB- cord-328082-7c7slfbp 185 72 c c NNP cord-328082-7c7slfbp 185 73 , , , cord-328082-7c7slfbp 185 74 e e NNP cord-328082-7c7slfbp 185 75 , , , cord-328082-7c7slfbp 185 76 and and CC cord-328082-7c7slfbp 185 77 g g NN cord-328082-7c7slfbp 185 78 , , , cord-328082-7c7slfbp 185 79 respectively respectively RB cord-328082-7c7slfbp 185 80 ) ) -RRB- cord-328082-7c7slfbp 185 81 , , , cord-328082-7c7slfbp 185 82 fixed fix VBN cord-328082-7c7slfbp 185 83 , , , cord-328082-7c7slfbp 185 84 and and CC cord-328082-7c7slfbp 185 85 processed process VBN cord-328082-7c7slfbp 185 86 for for IN cord-328082-7c7slfbp 185 87 immunofluoreseence immunofluoreseence NN cord-328082-7c7slfbp 185 88 microscopy microscopy NNS cord-328082-7c7slfbp 185 89 . . . cord-328082-7c7slfbp 186 1 In in IN cord-328082-7c7slfbp 186 2 all all DT cord-328082-7c7slfbp 186 3 cases case NNS cord-328082-7c7slfbp 186 4 50 50 CD cord-328082-7c7slfbp 186 5 /tg /tg ADD cord-328082-7c7slfbp 186 6 / / SYM cord-328082-7c7slfbp 186 7 ml ml NN cord-328082-7c7slfbp 186 8 of of IN cord-328082-7c7slfbp 186 9 cycloheximide cycloheximide NN cord-328082-7c7slfbp 186 10 was be VBD cord-328082-7c7slfbp 186 11 supplemented supplement VBN cord-328082-7c7slfbp 186 12 to to TO cord-328082-7c7slfbp 186 13 prevent prevent VB cord-328082-7c7slfbp 186 14 further further JJ cord-328082-7c7slfbp 186 15 synthesis synthesis NN cord-328082-7c7slfbp 186 16 of of IN cord-328082-7c7slfbp 186 17 man man NN cord-328082-7c7slfbp 186 18 II II NNP cord-328082-7c7slfbp 186 19 . . . cord-328082-7c7slfbp 187 1 Normal normal JJ cord-328082-7c7slfbp 187 2 distribution distribution NN cord-328082-7c7slfbp 187 3 of of IN cord-328082-7c7slfbp 187 4 man man NN cord-328082-7c7slfbp 187 5 II II NNP cord-328082-7c7slfbp 187 6 in in IN cord-328082-7c7slfbp 187 7 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 187 8 cells cell NNS cord-328082-7c7slfbp 187 9 ( ( -LRB- cord-328082-7c7slfbp 187 10 a a LS cord-328082-7c7slfbp 187 11 ) ) -RRB- cord-328082-7c7slfbp 187 12 . . . cord-328082-7c7slfbp 188 1 In in IN cord-328082-7c7slfbp 188 2 b b DT cord-328082-7c7slfbp 188 3 man man NN cord-328082-7c7slfbp 188 4 II II NNP cord-328082-7c7slfbp 188 5 is be VBZ cord-328082-7c7slfbp 188 6 observed observe VBN cord-328082-7c7slfbp 188 7 to to TO cord-328082-7c7slfbp 188 8 label label VB cord-328082-7c7slfbp 188 9 the the DT cord-328082-7c7slfbp 188 10 ER ER NNP cord-328082-7c7slfbp 188 11 after after IN cord-328082-7c7slfbp 188 12 a a DT cord-328082-7c7slfbp 188 13 90-rain 90-rain CD cord-328082-7c7slfbp 188 14 BFA BFA NNP cord-328082-7c7slfbp 188 15 treatment treatment NN cord-328082-7c7slfbp 188 16 at at IN cord-328082-7c7slfbp 188 17 37"C. 37"C. NNP cord-328082-7c7slfbp 189 1 In in IN cord-328082-7c7slfbp 189 2 caffeinetreated caffeinetreated JJ cord-328082-7c7slfbp 189 3 cells cell NNS cord-328082-7c7slfbp 189 4 at at IN cord-328082-7c7slfbp 189 5 20~ 20~ CD cord-328082-7c7slfbp 189 6 the the DT cord-328082-7c7slfbp 189 7 BFAinduced BFAinduced NNP cord-328082-7c7slfbp 189 8 transloeation transloeation NN cord-328082-7c7slfbp 189 9 of of IN cord-328082-7c7slfbp 189 10 man man NNP cord-328082-7c7slfbp 189 11 II II NNP cord-328082-7c7slfbp 189 12 to to IN cord-328082-7c7slfbp 189 13 the the DT cord-328082-7c7slfbp 189 14 ER ER NNP cord-328082-7c7slfbp 189 15 is be VBZ cord-328082-7c7slfbp 189 16 not not RB cord-328082-7c7slfbp 189 17 inhibited inhibit VBN cord-328082-7c7slfbp 189 18 ( ( -LRB- cord-328082-7c7slfbp 189 19 d d XX cord-328082-7c7slfbp 189 20 , , , cord-328082-7c7slfbp 189 21 60 60 CD cord-328082-7c7slfbp 189 22 rain;f rain;f NNP cord-328082-7c7slfbp 189 23 , , , cord-328082-7c7slfbp 189 24 120 120 CD cord-328082-7c7slfbp 189 25 rain rain NN cord-328082-7c7slfbp 189 26 ; ; : cord-328082-7c7slfbp 189 27 andh andh NN cord-328082-7c7slfbp 189 28 , , , cord-328082-7c7slfbp 189 29 180 180 CD cord-328082-7c7slfbp 189 30 min min NN cord-328082-7c7slfbp 189 31 ) ) -RRB- cord-328082-7c7slfbp 189 32 . . . cord-328082-7c7slfbp 190 1 When when WRB cord-328082-7c7slfbp 190 2 compared compare VBN cord-328082-7c7slfbp 190 3 with with IN cord-328082-7c7slfbp 190 4 the the DT cord-328082-7c7slfbp 190 5 control control NN cord-328082-7c7slfbp 190 6 cells cell NNS cord-328082-7c7slfbp 190 7 ( ( -LRB- cord-328082-7c7slfbp 190 8 c c NN cord-328082-7c7slfbp 190 9 , , , cord-328082-7c7slfbp 190 10 60 60 CD cord-328082-7c7slfbp 190 11 min min NN cord-328082-7c7slfbp 190 12 ; ; : cord-328082-7c7slfbp 190 13 e e NN cord-328082-7c7slfbp 190 14 , , , cord-328082-7c7slfbp 190 15 120 120 CD cord-328082-7c7slfbp 190 16 rain rain NN cord-328082-7c7slfbp 190 17 ; ; : cord-328082-7c7slfbp 190 18 and and CC cord-328082-7c7slfbp 190 19 g g NN cord-328082-7c7slfbp 190 20 , , , cord-328082-7c7slfbp 190 21 180 180 CD cord-328082-7c7slfbp 190 22 rain rain NN cord-328082-7c7slfbp 190 23 ) ) -RRB- cord-328082-7c7slfbp 191 1 the the DT cord-328082-7c7slfbp 191 2 BFA BFA NNP cord-328082-7c7slfbp 191 3 - - HYPH cord-328082-7c7slfbp 191 4 induced induce VBN cord-328082-7c7slfbp 191 5 disappearance disappearance NN cord-328082-7c7slfbp 191 6 of of IN cord-328082-7c7slfbp 191 7 the the DT cord-328082-7c7slfbp 191 8 perinuclear perinuclear NNP cord-328082-7c7slfbp 191 9 Golgi Golgi NNP cord-328082-7c7slfbp 191 10 labeling labeling NN cord-328082-7c7slfbp 191 11 of of IN cord-328082-7c7slfbp 191 12 man man NN cord-328082-7c7slfbp 191 13 II II NNP cord-328082-7c7slfbp 191 14 seems seem VBZ cord-328082-7c7slfbp 191 15 to to TO cord-328082-7c7slfbp 191 16 happen happen VB cord-328082-7c7slfbp 191 17 more more RBR cord-328082-7c7slfbp 191 18 slowly slowly RB cord-328082-7c7slfbp 191 19 in in IN cord-328082-7c7slfbp 191 20 the the DT cord-328082-7c7slfbp 191 21 caffeinetreated caffeinetreated JJ cord-328082-7c7slfbp 191 22 ceils ceil NNS cord-328082-7c7slfbp 191 23 than than IN cord-328082-7c7slfbp 191 24 in in IN cord-328082-7c7slfbp 191 25 the the DT cord-328082-7c7slfbp 191 26 control control NN cord-328082-7c7slfbp 191 27 cells cell NNS cord-328082-7c7slfbp 191 28 . . . cord-328082-7c7slfbp 192 1 Bar bar NN cord-328082-7c7slfbp 192 2 , , , cord-328082-7c7slfbp 192 3 20 20 CD cord-328082-7c7slfbp 192 4 ~m ~m JJ cord-328082-7c7slfbp 192 5 . . . cord-328082-7c7slfbp 193 1 presence presence NN cord-328082-7c7slfbp 193 2 of of IN cord-328082-7c7slfbp 193 3 BFA BFA NNP cord-328082-7c7slfbp 193 4 only only RB cord-328082-7c7slfbp 193 5 ( ( -LRB- cord-328082-7c7slfbp 193 6 Fig Fig NNP cord-328082-7c7slfbp 193 7 . . . cord-328082-7c7slfbp 193 8 4 4 CD cord-328082-7c7slfbp 193 9 e e CD cord-328082-7c7slfbp 193 10 ) ) -RRB- cord-328082-7c7slfbp 193 11 , , , cord-328082-7c7slfbp 193 12 in in IN cord-328082-7c7slfbp 193 13 41 41 CD cord-328082-7c7slfbp 193 14 % % NN cord-328082-7c7slfbp 193 15 of of IN cord-328082-7c7slfbp 193 16 the the DT cord-328082-7c7slfbp 193 17 cells cell NNS cord-328082-7c7slfbp 193 18 man man NN cord-328082-7c7slfbp 193 19 II II NNP cord-328082-7c7slfbp 193 20 had have VBD cord-328082-7c7slfbp 193 21 a a DT cord-328082-7c7slfbp 193 22 perinuclear perinuclear JJ cord-328082-7c7slfbp 193 23 localization localization NN cord-328082-7c7slfbp 193 24 and and CC cord-328082-7c7slfbp 193 25 in in IN cord-328082-7c7slfbp 193 26 97 97 CD cord-328082-7c7slfbp 193 27 % % NN cord-328082-7c7slfbp 193 28 of of IN cord-328082-7c7slfbp 193 29 the the DT cord-328082-7c7slfbp 193 30 cells cell NNS cord-328082-7c7slfbp 193 31 it -PRON- PRP cord-328082-7c7slfbp 193 32 was be VBD cord-328082-7c7slfbp 193 33 found find VBN cord-328082-7c7slfbp 193 34 only only RB cord-328082-7c7slfbp 193 35 or or CC cord-328082-7c7slfbp 193 36 partly partly RB cord-328082-7c7slfbp 193 37 in in IN cord-328082-7c7slfbp 193 38 the the DT cord-328082-7c7slfbp 193 39 ER ER NNP cord-328082-7c7slfbp 193 40 . . . cord-328082-7c7slfbp 194 1 As as IN cord-328082-7c7slfbp 194 2 the the DT cord-328082-7c7slfbp 194 3 chase chase NN cord-328082-7c7slfbp 194 4 was be VBD cord-328082-7c7slfbp 194 5 continued continue VBN cord-328082-7c7slfbp 194 6 to to IN cord-328082-7c7slfbp 194 7 180 180 CD cord-328082-7c7slfbp 194 8 min min NN cord-328082-7c7slfbp 194 9 at at IN cord-328082-7c7slfbp 194 10 20~ 20~ CD cord-328082-7c7slfbp 194 11 in in IN cord-328082-7c7slfbp 194 12 the the DT cord-328082-7c7slfbp 194 13 presence presence NN cord-328082-7c7slfbp 194 14 of of IN cord-328082-7c7slfbp 194 15 5 5 CD cord-328082-7c7slfbp 194 16 # # $ cord-328082-7c7slfbp 194 17 g g NN cord-328082-7c7slfbp 194 18 / / SYM cord-328082-7c7slfbp 194 19 ml ml NN cord-328082-7c7slfbp 194 20 of of IN cord-328082-7c7slfbp 194 21 BFA BFA NNP cord-328082-7c7slfbp 194 22 ( ( -LRB- cord-328082-7c7slfbp 194 23 Fig Fig NNP cord-328082-7c7slfbp 194 24 . . . cord-328082-7c7slfbp 194 25 _SP cord-328082-7c7slfbp 195 1 4 4 LS cord-328082-7c7slfbp 195 2 g g NNP cord-328082-7c7slfbp 195 3 ) ) -RRB- cord-328082-7c7slfbp 195 4 , , , cord-328082-7c7slfbp 195 5 man man NN cord-328082-7c7slfbp 195 6 II II NNP cord-328082-7c7slfbp 195 7 was be VBD cord-328082-7c7slfbp 195 8 still still RB cord-328082-7c7slfbp 195 9 found find VBN cord-328082-7c7slfbp 195 10 in in IN cord-328082-7c7slfbp 195 11 47 47 CD cord-328082-7c7slfbp 195 12 % % NN cord-328082-7c7slfbp 195 13 of of IN cord-328082-7c7slfbp 195 14 the the DT cord-328082-7c7slfbp 195 15 cells cell NNS cord-328082-7c7slfbp 195 16 in in IN cord-328082-7c7slfbp 195 17 perinuclear perinuclear NN cord-328082-7c7slfbp 195 18 elements element NNS cord-328082-7c7slfbp 195 19 and and CC cord-328082-7c7slfbp 195 20 in in IN cord-328082-7c7slfbp 195 21 97 97 CD cord-328082-7c7slfbp 195 22 % % NN cord-328082-7c7slfbp 195 23 of of IN cord-328082-7c7slfbp 195 24 the the DT cord-328082-7c7slfbp 195 25 cells cell NNS cord-328082-7c7slfbp 195 26 only only RB cord-328082-7c7slfbp 195 27 or or CC cord-328082-7c7slfbp 195 28 partly partly RB cord-328082-7c7slfbp 195 29 in in IN cord-328082-7c7slfbp 195 30 the the DT cord-328082-7c7slfbp 195 31 ER ER NNP cord-328082-7c7slfbp 195 32 . . . cord-328082-7c7slfbp 196 1 When when WRB cord-328082-7c7slfbp 196 2 the the DT cord-328082-7c7slfbp 196 3 cells cell NNS cord-328082-7c7slfbp 196 4 were be VBD cord-328082-7c7slfbp 196 5 treated treat VBN cord-328082-7c7slfbp 196 6 with with IN cord-328082-7c7slfbp 196 7 5 5 CD cord-328082-7c7slfbp 196 8 / / SYM cord-328082-7c7slfbp 196 9 zg zg NNP cord-328082-7c7slfbp 196 10 / / , cord-328082-7c7slfbp 196 11 ml ml NN cord-328082-7c7slfbp 196 12 of of IN cord-328082-7c7slfbp 196 13 BFA BFA NNP cord-328082-7c7slfbp 196 14 supplemented supplement VBD cord-328082-7c7slfbp 196 15 with with IN cord-328082-7c7slfbp 196 16 10 10 CD cord-328082-7c7slfbp 196 17 mM mm CD cord-328082-7c7slfbp 196 18 caffeine caffeine NN cord-328082-7c7slfbp 196 19 at at IN cord-328082-7c7slfbp 196 20 20~ 20~ CD cord-328082-7c7slfbp 196 21 for for IN cord-328082-7c7slfbp 196 22 60 60 CD cord-328082-7c7slfbp 196 23 min min NN cord-328082-7c7slfbp 196 24 ( ( -LRB- cord-328082-7c7slfbp 196 25 Fig Fig NNP cord-328082-7c7slfbp 196 26 . . . cord-328082-7c7slfbp 196 27 4 4 CD cord-328082-7c7slfbp 196 28 d d NNP cord-328082-7c7slfbp 196 29 ) ) -RRB- cord-328082-7c7slfbp 196 30 , , , cord-328082-7c7slfbp 196 31 man man NN cord-328082-7c7slfbp 196 32 II II NNP cord-328082-7c7slfbp 196 33 was be VBD cord-328082-7c7slfbp 196 34 found find VBN cord-328082-7c7slfbp 196 35 in in IN cord-328082-7c7slfbp 196 36 the the DT cord-328082-7c7slfbp 196 37 ER ER NNP cord-328082-7c7slfbp 196 38 in in IN cord-328082-7c7slfbp 196 39 68 68 CD cord-328082-7c7slfbp 196 40 % % NN cord-328082-7c7slfbp 196 41 of of IN cord-328082-7c7slfbp 196 42 the the DT cord-328082-7c7slfbp 196 43 cells cell NNS cord-328082-7c7slfbp 196 44 and and CC cord-328082-7c7slfbp 196 45 in in IN cord-328082-7c7slfbp 196 46 the the DT cord-328082-7c7slfbp 196 47 perinuclear perinuclear NNP cord-328082-7c7slfbp 196 48 localization localization NN cord-328082-7c7slfbp 196 49 in in IN cord-328082-7c7slfbp 196 50 92 92 CD cord-328082-7c7slfbp 196 51 % % NN cord-328082-7c7slfbp 196 52 of of IN cord-328082-7c7slfbp 196 53 the the DT cord-328082-7c7slfbp 196 54 cells cell NNS cord-328082-7c7slfbp 196 55 . . . cord-328082-7c7slfbp 197 1 After after IN cord-328082-7c7slfbp 197 2 a a DT cord-328082-7c7slfbp 197 3 120-min 120-min CD cord-328082-7c7slfbp 197 4 chase chase NN cord-328082-7c7slfbp 197 5 in in IN cord-328082-7c7slfbp 197 6 the the DT cord-328082-7c7slfbp 197 7 presence presence NN cord-328082-7c7slfbp 197 8 of of IN cord-328082-7c7slfbp 197 9 caffeine caffeine NN cord-328082-7c7slfbp 197 10 , , , cord-328082-7c7slfbp 197 11 man man NN cord-328082-7c7slfbp 197 12 II II NNP cord-328082-7c7slfbp 197 13 was be VBD cord-328082-7c7slfbp 197 14 located locate VBN cord-328082-7c7slfbp 197 15 partly partly RB cord-328082-7c7slfbp 197 16 or or CC cord-328082-7c7slfbp 197 17 completely completely RB cord-328082-7c7slfbp 197 18 in in IN cord-328082-7c7slfbp 197 19 the the DT cord-328082-7c7slfbp 197 20 ER ER NNP cord-328082-7c7slfbp 197 21 in in IN cord-328082-7c7slfbp 197 22 91 91 CD cord-328082-7c7slfbp 197 23 % % NN cord-328082-7c7slfbp 197 24 of of IN cord-328082-7c7slfbp 197 25 the the DT cord-328082-7c7slfbp 197 26 cells cell NNS cord-328082-7c7slfbp 197 27 and and CC cord-328082-7c7slfbp 197 28 was be VBD cord-328082-7c7slfbp 197 29 perinuclearly perinuclearly RB cord-328082-7c7slfbp 197 30 located locate VBN cord-328082-7c7slfbp 197 31 in in IN cord-328082-7c7slfbp 197 32 75 75 CD cord-328082-7c7slfbp 197 33 % % NN cord-328082-7c7slfbp 197 34 of of IN cord-328082-7c7slfbp 197 35 the the DT cord-328082-7c7slfbp 197 36 cells cell NNS cord-328082-7c7slfbp 197 37 ( ( -LRB- cord-328082-7c7slfbp 197 38 Fig Fig NNP cord-328082-7c7slfbp 197 39 . . . cord-328082-7c7slfbp 197 40 4 4 CD cord-328082-7c7slfbp 197 41 f f NNP cord-328082-7c7slfbp 197 42 ) ) -RRB- cord-328082-7c7slfbp 197 43 . . . cord-328082-7c7slfbp 198 1 When when WRB cord-328082-7c7slfbp 198 2 the the DT cord-328082-7c7slfbp 198 3 chase chase NN cord-328082-7c7slfbp 198 4 was be VBD cord-328082-7c7slfbp 198 5 carried carry VBN cord-328082-7c7slfbp 198 6 out out RP cord-328082-7c7slfbp 198 7 for for IN cord-328082-7c7slfbp 198 8 180 180 CD cord-328082-7c7slfbp 198 9 min min NN cord-328082-7c7slfbp 198 10 ( ( -LRB- cord-328082-7c7slfbp 198 11 Fig Fig NNP cord-328082-7c7slfbp 198 12 . . . cord-328082-7c7slfbp 198 13 4 4 CD cord-328082-7c7slfbp 198 14 h h NN cord-328082-7c7slfbp 198 15 ) ) -RRB- cord-328082-7c7slfbp 198 16 , , , cord-328082-7c7slfbp 198 17 man man NN cord-328082-7c7slfbp 198 18 II II NNP cord-328082-7c7slfbp 198 19 was be VBD cord-328082-7c7slfbp 198 20 found find VBN cord-328082-7c7slfbp 198 21 in in IN cord-328082-7c7slfbp 198 22 the the DT cord-328082-7c7slfbp 198 23 ER ER NNP cord-328082-7c7slfbp 198 24 in in IN cord-328082-7c7slfbp 198 25 96 96 CD cord-328082-7c7slfbp 198 26 % % NN cord-328082-7c7slfbp 198 27 of of IN cord-328082-7c7slfbp 198 28 the the DT cord-328082-7c7slfbp 198 29 cells cell NNS cord-328082-7c7slfbp 198 30 and and CC cord-328082-7c7slfbp 198 31 perinuclearly perinuclearly RB cord-328082-7c7slfbp 198 32 located locate VBN cord-328082-7c7slfbp 198 33 in in IN cord-328082-7c7slfbp 198 34 76 76 CD cord-328082-7c7slfbp 198 35 % % NN cord-328082-7c7slfbp 198 36 of of IN cord-328082-7c7slfbp 198 37 the the DT cord-328082-7c7slfbp 198 38 cells cell NNS cord-328082-7c7slfbp 198 39 . . . cord-328082-7c7slfbp 199 1 These these DT cord-328082-7c7slfbp 199 2 results result NNS cord-328082-7c7slfbp 199 3 show show VBP cord-328082-7c7slfbp 199 4 that that IN cord-328082-7c7slfbp 199 5 , , , cord-328082-7c7slfbp 199 6 after after IN cord-328082-7c7slfbp 199 7 60 60 CD cord-328082-7c7slfbp 199 8 min min NN cord-328082-7c7slfbp 199 9 in in IN cord-328082-7c7slfbp 199 10 the the DT cord-328082-7c7slfbp 199 11 presence presence NN cord-328082-7c7slfbp 199 12 of of IN cord-328082-7c7slfbp 199 13 caffeine caffeine NN cord-328082-7c7slfbp 199 14 , , , cord-328082-7c7slfbp 199 15 5 5 CD cord-328082-7c7slfbp 199 16 # # $ cord-328082-7c7slfbp 199 17 g g NN cord-328082-7c7slfbp 199 18 / / SYM cord-328082-7c7slfbp 199 19 ml ml NN cord-328082-7c7slfbp 199 20 of of IN cord-328082-7c7slfbp 199 21 BFA BFA NNP cord-328082-7c7slfbp 199 22 is be VBZ cord-328082-7c7slfbp 199 23 able able JJ cord-328082-7c7slfbp 199 24 to to TO cord-328082-7c7slfbp 199 25 translocate translocate VB cord-328082-7c7slfbp 199 26 man man NN cord-328082-7c7slfbp 199 27 II II NNP cord-328082-7c7slfbp 199 28 to to IN cord-328082-7c7slfbp 199 29 the the DT cord-328082-7c7slfbp 199 30 ER ER NNP cord-328082-7c7slfbp 199 31 0.76-fold 0.76-fold CD cord-328082-7c7slfbp 199 32 slower slow JJR cord-328082-7c7slfbp 199 33 compared compare VBN cord-328082-7c7slfbp 199 34 with with IN cord-328082-7c7slfbp 199 35 control control NN cord-328082-7c7slfbp 199 36 cells cell NNS cord-328082-7c7slfbp 199 37 . . . cord-328082-7c7slfbp 200 1 After after IN cord-328082-7c7slfbp 200 2 120 120 CD cord-328082-7c7slfbp 200 3 rain rain NN cord-328082-7c7slfbp 200 4 , , , cord-328082-7c7slfbp 200 5 however however RB cord-328082-7c7slfbp 200 6 , , , cord-328082-7c7slfbp 200 7 there there EX cord-328082-7c7slfbp 200 8 is be VBZ cord-328082-7c7slfbp 200 9 no no DT cord-328082-7c7slfbp 200 10 significant significant JJ cord-328082-7c7slfbp 200 11 difference difference NN cord-328082-7c7slfbp 200 12 between between IN cord-328082-7c7slfbp 200 13 caffeine caffeine NN cord-328082-7c7slfbp 200 14 - - HYPH cord-328082-7c7slfbp 200 15 treated treat VBN cord-328082-7c7slfbp 200 16 and and CC cord-328082-7c7slfbp 200 17 control control NN cord-328082-7c7slfbp 200 18 cells cell NNS cord-328082-7c7slfbp 200 19 in in IN cord-328082-7c7slfbp 200 20 the the DT cord-328082-7c7slfbp 200 21 number number NN cord-328082-7c7slfbp 200 22 of of IN cord-328082-7c7slfbp 200 23 cells cell NNS cord-328082-7c7slfbp 200 24 showing show VBG cord-328082-7c7slfbp 200 25 label label NN cord-328082-7c7slfbp 200 26 in in IN cord-328082-7c7slfbp 200 27 the the DT cord-328082-7c7slfbp 200 28 ER er NN cord-328082-7c7slfbp 200 29 . . . cord-328082-7c7slfbp 201 1 However however RB cord-328082-7c7slfbp 201 2 , , , cord-328082-7c7slfbp 201 3 in in IN cord-328082-7c7slfbp 201 4 caffeinetreated caffeinetreate VBN cord-328082-7c7slfbp 201 5 cells cell NNS cord-328082-7c7slfbp 201 6 the the DT cord-328082-7c7slfbp 201 7 disappearance disappearance NN cord-328082-7c7slfbp 201 8 of of IN cord-328082-7c7slfbp 201 9 the the DT cord-328082-7c7slfbp 201 10 perinuclear perinuclear NNP cord-328082-7c7slfbp 201 11 labeling labeling NN cord-328082-7c7slfbp 201 12 of of IN cord-328082-7c7slfbp 201 13 Figure Figure NNP cord-328082-7c7slfbp 201 14 5 5 CD cord-328082-7c7slfbp 201 15 . . . cord-328082-7c7slfbp 202 1 Caffeine caffeine NN cord-328082-7c7slfbp 202 2 - - HYPH cord-328082-7c7slfbp 202 3 induced induce VBN cord-328082-7c7slfbp 202 4 translocation translocation NN cord-328082-7c7slfbp 202 5 of of IN cord-328082-7c7slfbp 202 6 p58 p58 NN cord-328082-7c7slfbp 202 7 to to IN cord-328082-7c7slfbp 202 8 the the DT cord-328082-7c7slfbp 202 9 periphery periphery NN cord-328082-7c7slfbp 202 10 of of IN cord-328082-7c7slfbp 202 11 the the DT cord-328082-7c7slfbp 202 12 cells cell NNS cord-328082-7c7slfbp 202 13 . . . cord-328082-7c7slfbp 203 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 204 1 cells cell NNS cord-328082-7c7slfbp 204 2 were be VBD cord-328082-7c7slfbp 204 3 grown grow VBN cord-328082-7c7slfbp 204 4 for for IN cord-328082-7c7slfbp 204 5 30 30 CD cord-328082-7c7slfbp 204 6 min min NN cord-328082-7c7slfbp 204 7 at at IN cord-328082-7c7slfbp 204 8 20"C 20"c CD cord-328082-7c7slfbp 204 9 in in IN cord-328082-7c7slfbp 204 10 the the DT cord-328082-7c7slfbp 204 11 presence presence NN cord-328082-7c7slfbp 204 12 ( ( -LRB- cord-328082-7c7slfbp 204 13 a a NN cord-328082-7c7slfbp 204 14 and and CC cord-328082-7c7slfbp 204 15 b b LS cord-328082-7c7slfbp 204 16 ) ) -RRB- cord-328082-7c7slfbp 204 17 or or CC cord-328082-7c7slfbp 204 18 absence absence NN cord-328082-7c7slfbp 204 19 ( ( -LRB- cord-328082-7c7slfbp 204 20 c c NN cord-328082-7c7slfbp 204 21 and and CC cord-328082-7c7slfbp 204 22 d d NNP cord-328082-7c7slfbp 204 23 ) ) -RRB- cord-328082-7c7slfbp 204 24 of of IN cord-328082-7c7slfbp 204 25 10 10 CD cord-328082-7c7slfbp 204 26 mM mm CD cord-328082-7c7slfbp 204 27 caffeine caffeine NN cord-328082-7c7slfbp 204 28 , , , cord-328082-7c7slfbp 204 29 fixed fix VBN cord-328082-7c7slfbp 204 30 , , , cord-328082-7c7slfbp 204 31 and and CC cord-328082-7c7slfbp 204 32 double double RB cord-328082-7c7slfbp 204 33 - - HYPH cord-328082-7c7slfbp 204 34 labeled label VBN cord-328082-7c7slfbp 204 35 with with IN cord-328082-7c7slfbp 204 36 antibodies antibody NNS cord-328082-7c7slfbp 204 37 to to IN cord-328082-7c7slfbp 204 38 p58 p58 NNP cord-328082-7c7slfbp 204 39 ( ( -LRB- cord-328082-7c7slfbp 204 40 a a NN cord-328082-7c7slfbp 204 41 and and CC cord-328082-7c7slfbp 204 42 c c NN cord-328082-7c7slfbp 204 43 ) ) -RRB- cord-328082-7c7slfbp 204 44 and and CC cord-328082-7c7slfbp 204 45 man man NNP cord-328082-7c7slfbp 204 46 II II NNP cord-328082-7c7slfbp 204 47 ( ( -LRB- cord-328082-7c7slfbp 204 48 b b NN cord-328082-7c7slfbp 204 49 and and CC cord-328082-7c7slfbp 204 50 d d LS cord-328082-7c7slfbp 204 51 ) ) -RRB- cord-328082-7c7slfbp 204 52 . . . cord-328082-7c7slfbp 205 1 The the DT cord-328082-7c7slfbp 205 2 distribution distribution NN cord-328082-7c7slfbp 205 3 of of IN cord-328082-7c7slfbp 205 4 p58 p58 NN cord-328082-7c7slfbp 205 5 can can MD cord-328082-7c7slfbp 205 6 be be VB cord-328082-7c7slfbp 205 7 seen see VBN cord-328082-7c7slfbp 205 8 to to TO cord-328082-7c7slfbp 205 9 change change VB cord-328082-7c7slfbp 205 10 dramatically dramatically RB cord-328082-7c7slfbp 205 11 after after IN cord-328082-7c7slfbp 205 12 a a DT cord-328082-7c7slfbp 205 13 caffeine caffeine NN cord-328082-7c7slfbp 205 14 treatment treatment NN cord-328082-7c7slfbp 205 15 at at IN cord-328082-7c7slfbp 205 16 20"C. 20"c. CD cord-328082-7c7slfbp 206 1 In in IN cord-328082-7c7slfbp 206 2 a a DT cord-328082-7c7slfbp 206 3 , , , cord-328082-7c7slfbp 206 4 p58 p58 NNP cord-328082-7c7slfbp 206 5 can can MD cord-328082-7c7slfbp 206 6 be be VB cord-328082-7c7slfbp 206 7 found find VBN cord-328082-7c7slfbp 206 8 scattered scatter VBN cord-328082-7c7slfbp 206 9 through through IN cord-328082-7c7slfbp 206 10 the the DT cord-328082-7c7slfbp 206 11 cytoplasma cytoplasma NN cord-328082-7c7slfbp 206 12 in in IN cord-328082-7c7slfbp 206 13 contrast contrast NN cord-328082-7c7slfbp 206 14 to to TO cord-328082-7c7slfbp 206 15 control control VB cord-328082-7c7slfbp 206 16 cells cell NNS cord-328082-7c7slfbp 206 17 at at IN cord-328082-7c7slfbp 206 18 20~ 20~ CD cord-328082-7c7slfbp 206 19 ( ( -LRB- cord-328082-7c7slfbp 206 20 c c NN cord-328082-7c7slfbp 206 21 ) ) -RRB- cord-328082-7c7slfbp 206 22 , , , cord-328082-7c7slfbp 206 23 where where WRB cord-328082-7c7slfbp 206 24 p58 p58 NNP cord-328082-7c7slfbp 206 25 is be VBZ cord-328082-7c7slfbp 206 26 concentrated concentrate VBN cord-328082-7c7slfbp 206 27 in in IN cord-328082-7c7slfbp 206 28 the the DT cord-328082-7c7slfbp 206 29 perinuclear perinuclear JJ cord-328082-7c7slfbp 206 30 region region NN cord-328082-7c7slfbp 206 31 of of IN cord-328082-7c7slfbp 206 32 the the DT cord-328082-7c7slfbp 206 33 cell cell NN cord-328082-7c7slfbp 206 34 in in IN cord-328082-7c7slfbp 206 35 addition addition NN cord-328082-7c7slfbp 206 36 to to IN cord-328082-7c7slfbp 206 37 some some DT cord-328082-7c7slfbp 206 38 labeling labeling NN cord-328082-7c7slfbp 206 39 in in IN cord-328082-7c7slfbp 206 40 peripheral peripheral JJ cord-328082-7c7slfbp 206 41 vesicles vesicle NNS cord-328082-7c7slfbp 206 42 . . . cord-328082-7c7slfbp 207 1 When when WRB cord-328082-7c7slfbp 207 2 the the DT cord-328082-7c7slfbp 207 3 localization localization NN cord-328082-7c7slfbp 207 4 of of IN cord-328082-7c7slfbp 207 5 p58 p58 NNP cord-328082-7c7slfbp 207 6 is be VBZ cord-328082-7c7slfbp 207 7 compared compare VBN cord-328082-7c7slfbp 207 8 with with IN cord-328082-7c7slfbp 207 9 the the DT cord-328082-7c7slfbp 207 10 localization localization NN cord-328082-7c7slfbp 207 11 of of IN cord-328082-7c7slfbp 207 12 man man NN cord-328082-7c7slfbp 207 13 II II NNP cord-328082-7c7slfbp 207 14 in in IN cord-328082-7c7slfbp 207 15 caffeine caffeine NN cord-328082-7c7slfbp 207 16 - - HYPH cord-328082-7c7slfbp 207 17 treated treat VBN cord-328082-7c7slfbp 207 18 and and CC cord-328082-7c7slfbp 207 19 in in IN cord-328082-7c7slfbp 207 20 control control NN cord-328082-7c7slfbp 207 21 cells cell NNS cord-328082-7c7slfbp 207 22 incubated incubate VBN cord-328082-7c7slfbp 207 23 at at IN cord-328082-7c7slfbp 207 24 20~ 20~ CD cord-328082-7c7slfbp 207 25 p58 p58 CD cord-328082-7c7slfbp 207 26 can can MD cord-328082-7c7slfbp 207 27 be be VB cord-328082-7c7slfbp 207 28 seen see VBN cord-328082-7c7slfbp 207 29 to to TO cord-328082-7c7slfbp 207 30 colocalize colocalize VB cord-328082-7c7slfbp 207 31 only only RB cord-328082-7c7slfbp 207 32 randomly randomly RB cord-328082-7c7slfbp 207 33 with with IN cord-328082-7c7slfbp 207 34 man man NN cord-328082-7c7slfbp 207 35 II II NNP cord-328082-7c7slfbp 207 36 in in IN cord-328082-7c7slfbp 207 37 caffeine caffeine NN cord-328082-7c7slfbp 207 38 - - HYPH cord-328082-7c7slfbp 207 39 treated treat VBN cord-328082-7c7slfbp 207 40 cells cell NNS cord-328082-7c7slfbp 207 41 ( ( -LRB- cord-328082-7c7slfbp 207 42 a a DT cord-328082-7c7slfbp 207 43 for for IN cord-328082-7c7slfbp 207 44 p58 p58 NN cord-328082-7c7slfbp 207 45 and and CC cord-328082-7c7slfbp 207 46 b b LS cord-328082-7c7slfbp 207 47 for for IN cord-328082-7c7slfbp 207 48 man man NNP cord-328082-7c7slfbp 207 49 II II NNP cord-328082-7c7slfbp 207 50 ) ) -RRB- cord-328082-7c7slfbp 207 51 , , , cord-328082-7c7slfbp 207 52 but but CC cord-328082-7c7slfbp 207 53 to to TO cord-328082-7c7slfbp 207 54 colocalize colocalize VB cord-328082-7c7slfbp 207 55 a a DT cord-328082-7c7slfbp 207 56 great great JJ cord-328082-7c7slfbp 207 57 deal deal NN cord-328082-7c7slfbp 207 58 in in IN cord-328082-7c7slfbp 207 59 control control NN cord-328082-7c7slfbp 207 60 cells cell NNS cord-328082-7c7slfbp 207 61 ( ( -LRB- cord-328082-7c7slfbp 207 62 c c NN cord-328082-7c7slfbp 207 63 for for IN cord-328082-7c7slfbp 207 64 p58 p58 NNP cord-328082-7c7slfbp 207 65 and and CC cord-328082-7c7slfbp 207 66 d d NNP cord-328082-7c7slfbp 207 67 for for IN cord-328082-7c7slfbp 207 68 man man NNP cord-328082-7c7slfbp 207 69 II II NNP cord-328082-7c7slfbp 207 70 ) ) -RRB- cord-328082-7c7slfbp 207 71 . . . cord-328082-7c7slfbp 208 1 Also also RB cord-328082-7c7slfbp 208 2 , , , cord-328082-7c7slfbp 208 3 the the DT cord-328082-7c7slfbp 208 4 localization localization NN cord-328082-7c7slfbp 208 5 of of IN cord-328082-7c7slfbp 208 6 man man NN cord-328082-7c7slfbp 208 7 II II NNP cord-328082-7c7slfbp 208 8 seems seem VBZ cord-328082-7c7slfbp 208 9 not not RB cord-328082-7c7slfbp 208 10 to to TO cord-328082-7c7slfbp 208 11 be be VB cord-328082-7c7slfbp 208 12 affected affect VBN cord-328082-7c7slfbp 208 13 by by IN cord-328082-7c7slfbp 208 14 the the DT cord-328082-7c7slfbp 208 15 caffeine caffeine NN cord-328082-7c7slfbp 208 16 treatment treatment NN cord-328082-7c7slfbp 208 17 at at IN cord-328082-7c7slfbp 208 18 20~ 20~ CD cord-328082-7c7slfbp 208 19 ( ( -LRB- cord-328082-7c7slfbp 208 20 b b NN cord-328082-7c7slfbp 208 21 ) ) -RRB- cord-328082-7c7slfbp 208 22 . . . cord-328082-7c7slfbp 209 1 Bar bar NN cord-328082-7c7slfbp 209 2 , , , cord-328082-7c7slfbp 209 3 20 20 CD cord-328082-7c7slfbp 209 4 # # NN cord-328082-7c7slfbp 209 5 m. m. NN cord-328082-7c7slfbp 209 6 man man NN cord-328082-7c7slfbp 209 7 II II NNP cord-328082-7c7slfbp 209 8 seems seem VBZ cord-328082-7c7slfbp 209 9 to to TO cord-328082-7c7slfbp 209 10 occur occur VB cord-328082-7c7slfbp 209 11 more more RBR cord-328082-7c7slfbp 209 12 slowly slowly RB cord-328082-7c7slfbp 209 13 than than IN cord-328082-7c7slfbp 209 14 in in IN cord-328082-7c7slfbp 209 15 the the DT cord-328082-7c7slfbp 209 16 control control NN cord-328082-7c7slfbp 209 17 cells cell NNS cord-328082-7c7slfbp 209 18 at at IN cord-328082-7c7slfbp 209 19 the the DT cord-328082-7c7slfbp 209 20 60-min 60-min CD cord-328082-7c7slfbp 209 21 time time NN cord-328082-7c7slfbp 209 22 point point NN cord-328082-7c7slfbp 209 23 by by IN cord-328082-7c7slfbp 209 24 a a DT cord-328082-7c7slfbp 209 25 factor factor NN cord-328082-7c7slfbp 209 26 of of IN cord-328082-7c7slfbp 209 27 1.5 1.5 CD cord-328082-7c7slfbp 209 28 , , , cord-328082-7c7slfbp 209 29 at at IN cord-328082-7c7slfbp 209 30 the the DT cord-328082-7c7slfbp 209 31 120-min 120-min CD cord-328082-7c7slfbp 209 32 time time NN cord-328082-7c7slfbp 209 33 point point NN cord-328082-7c7slfbp 209 34 by by IN cord-328082-7c7slfbp 209 35 a a DT cord-328082-7c7slfbp 209 36 factor factor NN cord-328082-7c7slfbp 209 37 of of IN cord-328082-7c7slfbp 209 38 1.8 1.8 CD cord-328082-7c7slfbp 209 39 , , , cord-328082-7c7slfbp 209 40 and and CC cord-328082-7c7slfbp 209 41 at at IN cord-328082-7c7slfbp 209 42 the the DT cord-328082-7c7slfbp 209 43 180-min 180-min CD cord-328082-7c7slfbp 209 44 time time NN cord-328082-7c7slfbp 209 45 point point NN cord-328082-7c7slfbp 209 46 by by IN cord-328082-7c7slfbp 209 47 a a DT cord-328082-7c7slfbp 209 48 factor factor NN cord-328082-7c7slfbp 209 49 of of IN cord-328082-7c7slfbp 209 50 1.6 1.6 CD cord-328082-7c7slfbp 209 51 . . . cord-328082-7c7slfbp 210 1 Thus thus RB cord-328082-7c7slfbp 210 2 , , , cord-328082-7c7slfbp 210 3 these these DT cord-328082-7c7slfbp 210 4 results result NNS cord-328082-7c7slfbp 210 5 indicate indicate VBP cord-328082-7c7slfbp 210 6 that that IN cord-328082-7c7slfbp 210 7 caffeine caffeine NN cord-328082-7c7slfbp 210 8 does do VBZ cord-328082-7c7slfbp 210 9 not not RB cord-328082-7c7slfbp 210 10 inhibit inhibit VB cord-328082-7c7slfbp 210 11 the the DT cord-328082-7c7slfbp 210 12 effect effect NN cord-328082-7c7slfbp 210 13 of of IN cord-328082-7c7slfbp 210 14 BFA BFA NNP cord-328082-7c7slfbp 210 15 , , , cord-328082-7c7slfbp 210 16 but but CC cord-328082-7c7slfbp 210 17 it -PRON- PRP cord-328082-7c7slfbp 210 18 does do VBZ cord-328082-7c7slfbp 210 19 appear appear VB cord-328082-7c7slfbp 210 20 to to TO cord-328082-7c7slfbp 210 21 slow slow VB cord-328082-7c7slfbp 210 22 it -PRON- PRP cord-328082-7c7slfbp 210 23 down down RP cord-328082-7c7slfbp 210 24 somewhat somewhat RB cord-328082-7c7slfbp 210 25 . . . cord-328082-7c7slfbp 211 1 Interestingly interestingly RB cord-328082-7c7slfbp 211 2 , , , cord-328082-7c7slfbp 211 3 it -PRON- PRP cord-328082-7c7slfbp 211 4 seems seem VBZ cord-328082-7c7slfbp 211 5 that that IN cord-328082-7c7slfbp 211 6 during during IN cord-328082-7c7slfbp 211 7 a a DT cord-328082-7c7slfbp 211 8 180min 180min CD cord-328082-7c7slfbp 211 9 chase chase NN cord-328082-7c7slfbp 211 10 at at IN cord-328082-7c7slfbp 211 11 20~ 20~ CD cord-328082-7c7slfbp 211 12 both both DT cord-328082-7c7slfbp 211 13 in in IN cord-328082-7c7slfbp 211 14 caffeine caffeine NN cord-328082-7c7slfbp 211 15 - - HYPH cord-328082-7c7slfbp 211 16 treated treat VBN cord-328082-7c7slfbp 211 17 and and CC cord-328082-7c7slfbp 211 18 in in IN cord-328082-7c7slfbp 211 19 control control NN cord-328082-7c7slfbp 211 20 cells cell VBZ cord-328082-7c7slfbp 211 21 the the DT cord-328082-7c7slfbp 211 22 BFA BFA NNP cord-328082-7c7slfbp 211 23 - - HYPH cord-328082-7c7slfbp 211 24 induced induce VBN cord-328082-7c7slfbp 211 25 translocation translocation NN cord-328082-7c7slfbp 211 26 of of IN cord-328082-7c7slfbp 211 27 man man NN cord-328082-7c7slfbp 211 28 II II NNP cord-328082-7c7slfbp 211 29 reaches reach VBZ cord-328082-7c7slfbp 211 30 a a DT cord-328082-7c7slfbp 211 31 steady steady JJ cord-328082-7c7slfbp 211 32 - - HYPH cord-328082-7c7slfbp 211 33 state state NN cord-328082-7c7slfbp 211 34 situation situation NN cord-328082-7c7slfbp 211 35 after after IN cord-328082-7c7slfbp 211 36 a a DT cord-328082-7c7slfbp 211 37 120-min 120-min CD cord-328082-7c7slfbp 211 38 incubation incubation NN cord-328082-7c7slfbp 211 39 , , , cord-328082-7c7slfbp 211 40 whereafter whereafter RBR cord-328082-7c7slfbp 212 1 no no DT cord-328082-7c7slfbp 212 2 significant significant JJ cord-328082-7c7slfbp 212 3 chances chance NNS cord-328082-7c7slfbp 212 4 in in IN cord-328082-7c7slfbp 212 5 the the DT cord-328082-7c7slfbp 212 6 distribution distribution NN cord-328082-7c7slfbp 212 7 of of IN cord-328082-7c7slfbp 212 8 man man NN cord-328082-7c7slfbp 212 9 II II NNP cord-328082-7c7slfbp 212 10 in in IN cord-328082-7c7slfbp 212 11 the the DT cord-328082-7c7slfbp 212 12 cells cell NNS cord-328082-7c7slfbp 212 13 seems seem VBZ cord-328082-7c7slfbp 212 14 to to TO cord-328082-7c7slfbp 212 15 occur occur VB cord-328082-7c7slfbp 212 16 . . . cord-328082-7c7slfbp 213 1 These these DT cord-328082-7c7slfbp 213 2 results result NNS cord-328082-7c7slfbp 213 3 suggest suggest VBP cord-328082-7c7slfbp 213 4 that that IN cord-328082-7c7slfbp 213 5 even even RB cord-328082-7c7slfbp 213 6 though though IN cord-328082-7c7slfbp 213 7 caffeine caffeine NN cord-328082-7c7slfbp 213 8 inhibits inhibit VBZ cord-328082-7c7slfbp 213 9 the the DT cord-328082-7c7slfbp 213 10 movement movement NN cord-328082-7c7slfbp 213 11 of of IN cord-328082-7c7slfbp 213 12 membranes membrane NNS cord-328082-7c7slfbp 213 13 from from IN cord-328082-7c7slfbp 213 14 the the DT cord-328082-7c7slfbp 213 15 ER ER NNP cord-328082-7c7slfbp 213 16 to to IN cord-328082-7c7slfbp 213 17 the the DT cord-328082-7c7slfbp 213 18 Golgi Golgi NNP cord-328082-7c7slfbp 213 19 region region NN cord-328082-7c7slfbp 213 20 , , , cord-328082-7c7slfbp 213 21 it -PRON- PRP cord-328082-7c7slfbp 213 22 does do VBZ cord-328082-7c7slfbp 213 23 not not RB cord-328082-7c7slfbp 213 24 inhibit inhibit VB cord-328082-7c7slfbp 213 25 the the DT cord-328082-7c7slfbp 213 26 BFA BFA NNP cord-328082-7c7slfbp 213 27 - - HYPH cord-328082-7c7slfbp 213 28 induced induce VBN cord-328082-7c7slfbp 213 29 retrograde retrograde JJ cord-328082-7c7slfbp 213 30 movement movement NN cord-328082-7c7slfbp 213 31 of of IN cord-328082-7c7slfbp 213 32 Golgi Golgi NNP cord-328082-7c7slfbp 213 33 membranes membrane NNS cord-328082-7c7slfbp 213 34 to to IN cord-328082-7c7slfbp 213 35 the the DT cord-328082-7c7slfbp 213 36 ER ER NNP cord-328082-7c7slfbp 213 37 at at IN cord-328082-7c7slfbp 213 38 20~ 20~ CD cord-328082-7c7slfbp 213 39 Since since IN cord-328082-7c7slfbp 213 40 10 10 CD cord-328082-7c7slfbp 213 41 mM mm CD cord-328082-7c7slfbp 213 42 caffeine caffeine NN cord-328082-7c7slfbp 213 43 at at IN cord-328082-7c7slfbp 213 44 20~ 20~ CD cord-328082-7c7slfbp 213 45 did do VBD cord-328082-7c7slfbp 213 46 not not RB cord-328082-7c7slfbp 213 47 prevent prevent VB cord-328082-7c7slfbp 213 48 the the DT cord-328082-7c7slfbp 213 49 BFA BFA NNP cord-328082-7c7slfbp 213 50 - - HYPH cord-328082-7c7slfbp 213 51 induced induce VBN cord-328082-7c7slfbp 213 52 retrograde retrograde JJ cord-328082-7c7slfbp 213 53 movement movement NN cord-328082-7c7slfbp 213 54 of of IN cord-328082-7c7slfbp 213 55 Golgi Golgi NNP cord-328082-7c7slfbp 213 56 membranes membrane NNS cord-328082-7c7slfbp 213 57 to to IN cord-328082-7c7slfbp 213 58 the the DT cord-328082-7c7slfbp 213 59 ER ER NNP cord-328082-7c7slfbp 213 60 , , , cord-328082-7c7slfbp 213 61 it -PRON- PRP cord-328082-7c7slfbp 213 62 was be VBD cord-328082-7c7slfbp 213 63 of of IN cord-328082-7c7slfbp 213 64 interest interest NN cord-328082-7c7slfbp 213 65 to to TO cord-328082-7c7slfbp 213 66 study study VB cord-328082-7c7slfbp 213 67 the the DT cord-328082-7c7slfbp 213 68 distribution distribution NN cord-328082-7c7slfbp 213 69 of of IN cord-328082-7c7slfbp 213 70 the the DT cord-328082-7c7slfbp 213 71 pre pre JJ cord-328082-7c7slfbp 213 72 - - JJ cord-328082-7c7slfbp 213 73 Golgi Golgi NNP cord-328082-7c7slfbp 213 74 and and CC cord-328082-7c7slfbp 213 75 the the DT cord-328082-7c7slfbp 213 76 Golgi Golgi NNP cord-328082-7c7slfbp 213 77 membranes membrane NNS cord-328082-7c7slfbp 213 78 under under IN cord-328082-7c7slfbp 213 79 conditions condition NNS cord-328082-7c7slfbp 213 80 where where WRB cord-328082-7c7slfbp 213 81 ER er NN cord-328082-7c7slfbp 213 82 - - HYPH cord-328082-7c7slfbp 213 83 exit exit NN cord-328082-7c7slfbp 213 84 was be VBD cord-328082-7c7slfbp 213 85 inhibited inhibit VBN cord-328082-7c7slfbp 213 86 . . . cord-328082-7c7slfbp 214 1 When when WRB cord-328082-7c7slfbp 214 2 the the DT cord-328082-7c7slfbp 214 3 anterograde anterograde JJ cord-328082-7c7slfbp 214 4 but but CC cord-328082-7c7slfbp 214 5 not not RB cord-328082-7c7slfbp 214 6 the the DT cord-328082-7c7slfbp 214 7 retrograde retrograde JJ cord-328082-7c7slfbp 214 8 membrane membrane NN cord-328082-7c7slfbp 214 9 traffic traffic NN cord-328082-7c7slfbp 214 10 between between IN cord-328082-7c7slfbp 214 11 the the DT cord-328082-7c7slfbp 214 12 ER ER NNP cord-328082-7c7slfbp 214 13 and and CC cord-328082-7c7slfbp 214 14 the the DT cord-328082-7c7slfbp 214 15 Golgi Golgi NNP cord-328082-7c7slfbp 214 16 is be VBZ cord-328082-7c7slfbp 214 17 inhibited inhibit VBN cord-328082-7c7slfbp 214 18 by by IN cord-328082-7c7slfbp 214 19 caffeine caffeine NN cord-328082-7c7slfbp 214 20 , , , cord-328082-7c7slfbp 214 21 two two CD cord-328082-7c7slfbp 214 22 things thing NNS cord-328082-7c7slfbp 214 23 could could MD cord-328082-7c7slfbp 214 24 happen happen VB cord-328082-7c7slfbp 214 25 to to IN cord-328082-7c7slfbp 214 26 the the DT cord-328082-7c7slfbp 214 27 Golgi Golgi NNP cord-328082-7c7slfbp 214 28 membranes membrane NNS cord-328082-7c7slfbp 214 29 : : : cord-328082-7c7slfbp 214 30 all all PDT cord-328082-7c7slfbp 214 31 the the DT cord-328082-7c7slfbp 214 32 Golgi Golgi NNP cord-328082-7c7slfbp 214 33 membranes membrane NNS cord-328082-7c7slfbp 214 34 would would MD cord-328082-7c7slfbp 214 35 recycle recycle VB cord-328082-7c7slfbp 214 36 to to IN cord-328082-7c7slfbp 214 37 the the DT cord-328082-7c7slfbp 214 38 ER ER NNP cord-328082-7c7slfbp 214 39 as as IN cord-328082-7c7slfbp 214 40 in in IN cord-328082-7c7slfbp 214 41 the the DT cord-328082-7c7slfbp 214 42 case case NN cord-328082-7c7slfbp 214 43 of of IN cord-328082-7c7slfbp 214 44 BFA BFA NNP cord-328082-7c7slfbp 214 45 or or CC cord-328082-7c7slfbp 214 46 part part NN cord-328082-7c7slfbp 214 47 of of IN cord-328082-7c7slfbp 214 48 the the DT cord-328082-7c7slfbp 214 49 Golgi Golgi NNP cord-328082-7c7slfbp 214 50 would would MD cord-328082-7c7slfbp 214 51 do do VB cord-328082-7c7slfbp 214 52 so so RB cord-328082-7c7slfbp 214 53 . . . cord-328082-7c7slfbp 215 1 As as IN cord-328082-7c7slfbp 215 2 markers marker NNS cord-328082-7c7slfbp 215 3 for for IN cord-328082-7c7slfbp 215 4 Golgi Golgi NNP cord-328082-7c7slfbp 215 5 membranes membrane NNS cord-328082-7c7slfbp 215 6 we -PRON- PRP cord-328082-7c7slfbp 215 7 used use VBD cord-328082-7c7slfbp 215 8 p58 p58 NNP cord-328082-7c7slfbp 215 9 protein protein NN cord-328082-7c7slfbp 215 10 , , , cord-328082-7c7slfbp 215 11 known know VBN cord-328082-7c7slfbp 215 12 to to TO cord-328082-7c7slfbp 215 13 be be VB cord-328082-7c7slfbp 215 14 localized localize VBN cord-328082-7c7slfbp 215 15 in in IN cord-328082-7c7slfbp 215 16 various various JJ cord-328082-7c7slfbp 215 17 cell cell NN cord-328082-7c7slfbp 215 18 types type NNS cord-328082-7c7slfbp 215 19 in in IN cord-328082-7c7slfbp 215 20 the the DT cord-328082-7c7slfbp 215 21 pre pre JJ cord-328082-7c7slfbp 215 22 - - JJ cord-328082-7c7slfbp 215 23 Golgi golgi JJ cord-328082-7c7slfbp 215 24 elements element NNS cord-328082-7c7slfbp 215 25 and and CC cord-328082-7c7slfbp 215 26 in in IN cord-328082-7c7slfbp 215 27 cis cis NNP cord-328082-7c7slfbp 215 28 - - HYPH cord-328082-7c7slfbp 215 29 Golgi Golgi NNP cord-328082-7c7slfbp 215 30 ( ( -LRB- cord-328082-7c7slfbp 215 31 Saraste Saraste NNP cord-328082-7c7slfbp 215 32 et et NNP cord-328082-7c7slfbp 215 33 al al NNP cord-328082-7c7slfbp 215 34 . . NNP cord-328082-7c7slfbp 215 35 , , , cord-328082-7c7slfbp 215 36 1987 1987 CD cord-328082-7c7slfbp 215 37 ; ; : cord-328082-7c7slfbp 215 38 Saraste Saraste NNP cord-328082-7c7slfbp 215 39 and and CC cord-328082-7c7slfbp 215 40 Svensson Svensson NNP cord-328082-7c7slfbp 215 41 1991 1991 CD cord-328082-7c7slfbp 215 42 ; ; : cord-328082-7c7slfbp 215 43 Lahtinen Lahtinen NNP cord-328082-7c7slfbp 215 44 et et NNP cord-328082-7c7slfbp 215 45 al al NNP cord-328082-7c7slfbp 215 46 . . NNP cord-328082-7c7slfbp 215 47 , , , cord-328082-7c7slfbp 215 48 1992 1992 CD cord-328082-7c7slfbp 215 49 ) ) -RRB- cord-328082-7c7slfbp 215 50 , , , cord-328082-7c7slfbp 215 51 and and CC cord-328082-7c7slfbp 215 52 a a DT cord-328082-7c7slfbp 215 53 135-kD 135-kd CD cord-328082-7c7slfbp 215 54 integral integral JJ cord-328082-7c7slfbp 215 55 Golgi Golgi NNP cord-328082-7c7slfbp 215 56 stack stack NN cord-328082-7c7slfbp 215 57 membrane membrane NN cord-328082-7c7slfbp 215 58 protein protein NN cord-328082-7c7slfbp 215 59 Burke Burke NNP cord-328082-7c7slfbp 215 60 et et NNP cord-328082-7c7slfbp 215 61 al al NNP cord-328082-7c7slfbp 215 62 . . NNP cord-328082-7c7slfbp 215 63 , , , cord-328082-7c7slfbp 215 64 1982 1982 CD cord-328082-7c7slfbp 215 65 ) ) -RRB- cord-328082-7c7slfbp 215 66 which which WDT cord-328082-7c7slfbp 215 67 has have VBZ cord-328082-7c7slfbp 215 68 recently recently RB cord-328082-7c7slfbp 215 69 been be VBN cord-328082-7c7slfbp 215 70 shown show VBN cord-328082-7c7slfbp 215 71 to to TO cord-328082-7c7slfbp 215 72 be be VB cord-328082-7c7slfbp 215 73 identical identical JJ cord-328082-7c7slfbp 215 74 with with IN cord-328082-7c7slfbp 215 75 man man NN cord-328082-7c7slfbp 215 76 II II NNP cord-328082-7c7slfbp 215 77 ( ( -LRB- cord-328082-7c7slfbp 215 78 Baron Baron NNP cord-328082-7c7slfbp 215 79 and and CC cord-328082-7c7slfbp 215 80 Garoff Garoff NNP cord-328082-7c7slfbp 215 81 , , , cord-328082-7c7slfbp 215 82 1990 1990 CD cord-328082-7c7slfbp 215 83 ) ) -RRB- cord-328082-7c7slfbp 215 84 . . . cord-328082-7c7slfbp 216 1 BHK-21 bhk-21 DT cord-328082-7c7slfbp 216 2 cells cell NNS cord-328082-7c7slfbp 216 3 were be VBD cord-328082-7c7slfbp 216 4 treated treat VBN cord-328082-7c7slfbp 216 5 for for IN cord-328082-7c7slfbp 216 6 30 30 CD cord-328082-7c7slfbp 216 7 , , , cord-328082-7c7slfbp 216 8 60 60 CD cord-328082-7c7slfbp 216 9 , , , cord-328082-7c7slfbp 216 10 or or CC cord-328082-7c7slfbp 216 11 120 120 CD cord-328082-7c7slfbp 216 12 min min NN cord-328082-7c7slfbp 216 13 with with IN cord-328082-7c7slfbp 216 14 or or CC cord-328082-7c7slfbp 216 15 without without IN cord-328082-7c7slfbp 216 16 10 10 CD cord-328082-7c7slfbp 216 17 mM mm CD cord-328082-7c7slfbp 216 18 caffeine caffeine NN cord-328082-7c7slfbp 216 19 at at IN cord-328082-7c7slfbp 216 20 20~ 20~ CD cord-328082-7c7slfbp 216 21 fixed fix VBN cord-328082-7c7slfbp 216 22 , , , cord-328082-7c7slfbp 216 23 and and CC cord-328082-7c7slfbp 216 24 labeled label VBN cord-328082-7c7slfbp 216 25 with with IN cord-328082-7c7slfbp 216 26 a a DT cord-328082-7c7slfbp 216 27 polyclonal polyclonal JJ cord-328082-7c7slfbp 216 28 antibody antibody NN cord-328082-7c7slfbp 216 29 to to IN cord-328082-7c7slfbp 216 30 p58 p58 NN cord-328082-7c7slfbp 216 31 and and CC cord-328082-7c7slfbp 216 32 a a DT cord-328082-7c7slfbp 216 33 monoclonal monoclonal JJ cord-328082-7c7slfbp 216 34 antibody antibody NN cord-328082-7c7slfbp 216 35 to to TO cord-328082-7c7slfbp 216 36 man man VB cord-328082-7c7slfbp 216 37 II II NNP cord-328082-7c7slfbp 216 38 ( ( -LRB- cord-328082-7c7slfbp 216 39 135 135 CD cord-328082-7c7slfbp 216 40 kD kd ADD cord-328082-7c7slfbp 216 41 ) ) -RRB- cord-328082-7c7slfbp 216 42 . . . cord-328082-7c7slfbp 217 1 Surprisingly surprisingly RB cord-328082-7c7slfbp 217 2 , , , cord-328082-7c7slfbp 217 3 after after IN cord-328082-7c7slfbp 217 4 30 30 CD cord-328082-7c7slfbp 217 5 min min NN cord-328082-7c7slfbp 217 6 at at IN cord-328082-7c7slfbp 217 7 20~ 20~ CD cord-328082-7c7slfbp 217 8 in in IN cord-328082-7c7slfbp 217 9 the the DT cord-328082-7c7slfbp 217 10 presence presence NN cord-328082-7c7slfbp 217 11 of of IN cord-328082-7c7slfbp 217 12 Figure Figure NNP cord-328082-7c7slfbp 217 13 6 6 CD cord-328082-7c7slfbp 217 14 . . . cord-328082-7c7slfbp 218 1 The the DT cord-328082-7c7slfbp 218 2 caffeine caffeine NN cord-328082-7c7slfbp 218 3 - - HYPH cord-328082-7c7slfbp 218 4 induced induce VBN cord-328082-7c7slfbp 218 5 translocation translocation NN cord-328082-7c7slfbp 218 6 of of IN cord-328082-7c7slfbp 218 7 p58 p58 NN cord-328082-7c7slfbp 218 8 to to IN cord-328082-7c7slfbp 218 9 the the DT cord-328082-7c7slfbp 218 10 periphery periphery NN cord-328082-7c7slfbp 218 11 of of IN cord-328082-7c7slfbp 218 12 the the DT cord-328082-7c7slfbp 218 13 cells cell NNS cord-328082-7c7slfbp 218 14 is be VBZ cord-328082-7c7slfbp 218 15 reversible reversible JJ cord-328082-7c7slfbp 218 16 . . . cord-328082-7c7slfbp 219 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 220 1 cells cell NNS cord-328082-7c7slfbp 220 2 were be VBD cord-328082-7c7slfbp 220 3 incubated incubate VBN cord-328082-7c7slfbp 220 4 in in IN cord-328082-7c7slfbp 220 5 the the DT cord-328082-7c7slfbp 220 6 presence presence NN cord-328082-7c7slfbp 220 7 ( ( -LRB- cord-328082-7c7slfbp 220 8 20~ 20~ CD cord-328082-7c7slfbp 220 9 caffeine caffeine NN cord-328082-7c7slfbp 220 10 ) ) -RRB- cord-328082-7c7slfbp 220 11 or or CC cord-328082-7c7slfbp 220 12 absence absence NN cord-328082-7c7slfbp 220 13 ( ( -LRB- cord-328082-7c7slfbp 220 14 20 20 CD cord-328082-7c7slfbp 220 15 ~ ~ CD cord-328082-7c7slfbp 220 16 C C NNP cord-328082-7c7slfbp 220 17 control control NN cord-328082-7c7slfbp 220 18 ) ) -RRB- cord-328082-7c7slfbp 220 19 of of IN cord-328082-7c7slfbp 220 20 10 10 CD cord-328082-7c7slfbp 220 21 mM mm CD cord-328082-7c7slfbp 220 22 caffeine caffeine NN cord-328082-7c7slfbp 220 23 at at IN cord-328082-7c7slfbp 220 24 20~ 20~ CD cord-328082-7c7slfbp 220 25 for for IN cord-328082-7c7slfbp 220 26 60 60 CD cord-328082-7c7slfbp 220 27 rain rain NN cord-328082-7c7slfbp 220 28 . . . cord-328082-7c7slfbp 221 1 Cells cell NNS cord-328082-7c7slfbp 221 2 incubated incubate VBN cord-328082-7c7slfbp 221 3 at at IN cord-328082-7c7slfbp 221 4 20~ 20~ CD cord-328082-7c7slfbp 221 5 with with IN cord-328082-7c7slfbp 221 6 caffeine caffeine NN cord-328082-7c7slfbp 221 7 were be VBD cord-328082-7c7slfbp 221 8 further further RB cord-328082-7c7slfbp 221 9 incubated incubate VBN cord-328082-7c7slfbp 221 10 60 60 CD cord-328082-7c7slfbp 221 11 rain rain NN cord-328082-7c7slfbp 221 12 without without IN cord-328082-7c7slfbp 221 13 caffeine caffeine NN cord-328082-7c7slfbp 221 14 at at IN cord-328082-7c7slfbp 221 15 20~ 20~ CD cord-328082-7c7slfbp 221 16 ( ( -LRB- cord-328082-7c7slfbp 221 17 reversion reversion NN cord-328082-7c7slfbp 221 18 ) ) -RRB- cord-328082-7c7slfbp 221 19 , , , cord-328082-7c7slfbp 221 20 fixed fix VBN cord-328082-7c7slfbp 221 21 , , , cord-328082-7c7slfbp 221 22 and and CC cord-328082-7c7slfbp 221 23 doubled double VBN cord-328082-7c7slfbp 221 24 - - HYPH cord-328082-7c7slfbp 221 25 labeled label VBN cord-328082-7c7slfbp 221 26 with with IN cord-328082-7c7slfbp 221 27 antibodies antibody NNS cord-328082-7c7slfbp 221 28 to to IN cord-328082-7c7slfbp 221 29 p58 p58 NN cord-328082-7c7slfbp 221 30 and and CC cord-328082-7c7slfbp 221 31 man man NN cord-328082-7c7slfbp 221 32 II II NNP cord-328082-7c7slfbp 221 33 . . . cord-328082-7c7slfbp 222 1 no no DT cord-328082-7c7slfbp 222 2 drug drug NN cord-328082-7c7slfbp 222 3 shows show VBZ cord-328082-7c7slfbp 222 4 the the DT cord-328082-7c7slfbp 222 5 normal normal JJ cord-328082-7c7slfbp 222 6 distribution distribution NN cord-328082-7c7slfbp 222 7 of of IN cord-328082-7c7slfbp 222 8 p58 p58 NN cord-328082-7c7slfbp 222 9 and and CC cord-328082-7c7slfbp 222 10 man man NN cord-328082-7c7slfbp 222 11 II II NNP cord-328082-7c7slfbp 222 12 in in IN cord-328082-7c7slfbp 222 13 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 222 14 cells cell NNS cord-328082-7c7slfbp 222 15 . . . cord-328082-7c7slfbp 223 1 In in IN cord-328082-7c7slfbp 223 2 reversion reversion NN cord-328082-7c7slfbp 223 3 , , , cord-328082-7c7slfbp 223 4 the the DT cord-328082-7c7slfbp 223 5 perinuclear perinuclear NNP cord-328082-7c7slfbp 223 6 localization localization NN cord-328082-7c7slfbp 223 7 of of IN cord-328082-7c7slfbp 223 8 p58 p58 NNP cord-328082-7c7slfbp 223 9 , , , cord-328082-7c7slfbp 223 10 similar similar JJ cord-328082-7c7slfbp 223 11 to to IN cord-328082-7c7slfbp 223 12 that that DT cord-328082-7c7slfbp 223 13 in in IN cord-328082-7c7slfbp 223 14 20~ 20~ CD cord-328082-7c7slfbp 223 15 control control NN cord-328082-7c7slfbp 223 16 , , , cord-328082-7c7slfbp 223 17 is be VBZ cord-328082-7c7slfbp 223 18 restored restore VBN cord-328082-7c7slfbp 223 19 . . . cord-328082-7c7slfbp 224 1 Bar bar NN cord-328082-7c7slfbp 224 2 , , , cord-328082-7c7slfbp 224 3 20 20 CD cord-328082-7c7slfbp 224 4 # # NN cord-328082-7c7slfbp 224 5 m. m. NN cord-328082-7c7slfbp 224 6 caffeine caffeine NN cord-328082-7c7slfbp 224 7 , , , cord-328082-7c7slfbp 224 8 p58 p58 NNP cord-328082-7c7slfbp 224 9 was be VBD cord-328082-7c7slfbp 224 10 found find VBN cord-328082-7c7slfbp 224 11 scattered scatter VBN cord-328082-7c7slfbp 224 12 throughout throughout IN cord-328082-7c7slfbp 224 13 the the DT cord-328082-7c7slfbp 224 14 cytoplasm cytoplasm NN cord-328082-7c7slfbp 224 15 ( ( -LRB- cord-328082-7c7slfbp 224 16 Fig fig NN cord-328082-7c7slfbp 224 17 . . . cord-328082-7c7slfbp 225 1 5 5 LS cord-328082-7c7slfbp 225 2 a a LS cord-328082-7c7slfbp 225 3 ) ) -RRB- cord-328082-7c7slfbp 225 4 whereas whereas IN cord-328082-7c7slfbp 225 5 man man NN cord-328082-7c7slfbp 225 6 II II NNP cord-328082-7c7slfbp 225 7 still still RB cord-328082-7c7slfbp 225 8 remained remain VBD cord-328082-7c7slfbp 225 9 concentrated concentrated JJ cord-328082-7c7slfbp 225 10 in in IN cord-328082-7c7slfbp 225 11 a a DT cord-328082-7c7slfbp 225 12 perinuclear perinuclear JJ cord-328082-7c7slfbp 225 13 localization localization NN cord-328082-7c7slfbp 225 14 ( ( -LRB- cord-328082-7c7slfbp 225 15 Fig Fig NNP cord-328082-7c7slfbp 225 16 . . . cord-328082-7c7slfbp 225 17 5 5 CD cord-328082-7c7slfbp 225 18 b b LS cord-328082-7c7slfbp 225 19 ) ) -RRB- cord-328082-7c7slfbp 225 20 . . . cord-328082-7c7slfbp 226 1 Incubation incubation NN cord-328082-7c7slfbp 226 2 for for IN cord-328082-7c7slfbp 226 3 an an DT cord-328082-7c7slfbp 226 4 additional additional JJ cord-328082-7c7slfbp 226 5 30 30 CD cord-328082-7c7slfbp 226 6 or or CC cord-328082-7c7slfbp 226 7 90 90 CD cord-328082-7c7slfbp 226 8 min min NN cord-328082-7c7slfbp 226 9 showed show VBD cord-328082-7c7slfbp 226 10 similar similar JJ cord-328082-7c7slfbp 226 11 distribution distribution NN cord-328082-7c7slfbp 226 12 of of IN cord-328082-7c7slfbp 226 13 p58 p58 NN cord-328082-7c7slfbp 226 14 and and CC cord-328082-7c7slfbp 226 15 man man NNP cord-328082-7c7slfbp 226 16 II II NNP cord-328082-7c7slfbp 226 17 ( ( -LRB- cord-328082-7c7slfbp 226 18 data datum NNS cord-328082-7c7slfbp 226 19 not not RB cord-328082-7c7slfbp 226 20 shown show VBN cord-328082-7c7slfbp 226 21 ) ) -RRB- cord-328082-7c7slfbp 226 22 ( ( -LRB- cord-328082-7c7slfbp 226 23 see see VB cord-328082-7c7slfbp 226 24 also also RB cord-328082-7c7slfbp 226 25 Fig Fig NNP cord-328082-7c7slfbp 226 26 . . NNP cord-328082-7c7slfbp 226 27 6 6 CD cord-328082-7c7slfbp 226 28 for for IN cord-328082-7c7slfbp 226 29 a a DT cord-328082-7c7slfbp 226 30 60-rain 60-rain CD cord-328082-7c7slfbp 226 31 incubation incubation NN cord-328082-7c7slfbp 226 32 ) ) -RRB- cord-328082-7c7slfbp 226 33 . . . cord-328082-7c7slfbp 227 1 In in IN cord-328082-7c7slfbp 227 2 control control NN cord-328082-7c7slfbp 227 3 cells cell NNS cord-328082-7c7slfbp 227 4 , , , cord-328082-7c7slfbp 227 5 incubated incubate VBN cord-328082-7c7slfbp 227 6 for for IN cord-328082-7c7slfbp 227 7 30 30 CD cord-328082-7c7slfbp 227 8 rain rain NN cord-328082-7c7slfbp 227 9 at at IN cord-328082-7c7slfbp 227 10 20~ 20~ CD cord-328082-7c7slfbp 227 11 p58 p58 NN cord-328082-7c7slfbp 227 12 and and CC cord-328082-7c7slfbp 227 13 man man NN cord-328082-7c7slfbp 227 14 I1 i1 NN cord-328082-7c7slfbp 227 15 ( ( -LRB- cord-328082-7c7slfbp 227 16 Fig Fig NNP cord-328082-7c7slfbp 227 17 . . NNP cord-328082-7c7slfbp 227 18 5 5 CD cord-328082-7c7slfbp 227 19 , , , cord-328082-7c7slfbp 227 20 c c NN cord-328082-7c7slfbp 227 21 and and CC cord-328082-7c7slfbp 227 22 d d NNP cord-328082-7c7slfbp 227 23 , , , cord-328082-7c7slfbp 227 24 respectively respectively RB cord-328082-7c7slfbp 227 25 ) ) -RRB- cord-328082-7c7slfbp 227 26 were be VBD cord-328082-7c7slfbp 227 27 found find VBN cord-328082-7c7slfbp 227 28 to to IN cord-328082-7c7slfbp 227 29 colocalize colocalize NNP cord-328082-7c7slfbp 227 30 largely largely RB cord-328082-7c7slfbp 227 31 , , , cord-328082-7c7slfbp 227 32 except except IN cord-328082-7c7slfbp 227 33 for for IN cord-328082-7c7slfbp 227 34 the the DT cord-328082-7c7slfbp 227 35 peripheral peripheral JJ cord-328082-7c7slfbp 227 36 vesicles vesicle NNS cord-328082-7c7slfbp 227 37 positive positive JJ cord-328082-7c7slfbp 227 38 only only RB cord-328082-7c7slfbp 227 39 for for IN cord-328082-7c7slfbp 227 40 p58 p58 NNP cord-328082-7c7slfbp 227 41 . . . cord-328082-7c7slfbp 228 1 Fig Fig NNP cord-328082-7c7slfbp 228 2 . . . cord-328082-7c7slfbp 229 1 5 5 LS cord-328082-7c7slfbp 229 2 , , , cord-328082-7c7slfbp 229 3 a a NN cord-328082-7c7slfbp 229 4 and and CC cord-328082-7c7slfbp 229 5 c c NN cord-328082-7c7slfbp 229 6 , , , cord-328082-7c7slfbp 229 7 demonstrates demonstrate VBZ cord-328082-7c7slfbp 229 8 that that IN cord-328082-7c7slfbp 229 9 p58 p58 NNP cord-328082-7c7slfbp 229 10 is be VBZ cord-328082-7c7slfbp 229 11 translocated translocate VBN cord-328082-7c7slfbp 229 12 away away RB cord-328082-7c7slfbp 229 13 from from IN cord-328082-7c7slfbp 229 14 its -PRON- PRP$ cord-328082-7c7slfbp 229 15 normal normal JJ cord-328082-7c7slfbp 229 16 perinuclear perinuclear NN cord-328082-7c7slfbp 229 17 localization localization NN cord-328082-7c7slfbp 229 18 at at IN cord-328082-7c7slfbp 229 19 20~ 20~ CD cord-328082-7c7slfbp 229 20 in in IN cord-328082-7c7slfbp 229 21 the the DT cord-328082-7c7slfbp 229 22 presence presence NN cord-328082-7c7slfbp 229 23 of of IN cord-328082-7c7slfbp 229 24 10 10 CD cord-328082-7c7slfbp 229 25 mM mm CD cord-328082-7c7slfbp 229 26 caffeine caffeine NN cord-328082-7c7slfbp 229 27 . . . cord-328082-7c7slfbp 230 1 In in IN cord-328082-7c7slfbp 230 2 caffeine caffeine NN cord-328082-7c7slfbp 230 3 - - HYPH cord-328082-7c7slfbp 230 4 treated treat VBN cord-328082-7c7slfbp 230 5 cells cell NNS cord-328082-7c7slfbp 230 6 , , , cord-328082-7c7slfbp 230 7 the the DT cord-328082-7c7slfbp 230 8 colocalization colocalization NN cord-328082-7c7slfbp 230 9 of of IN cord-328082-7c7slfbp 230 10 p58 p58 NNP cord-328082-7c7slfbp 230 11 ( ( -LRB- cord-328082-7c7slfbp 230 12 Fig Fig NNP cord-328082-7c7slfbp 230 13 . . . cord-328082-7c7slfbp 230 14 5 5 CD cord-328082-7c7slfbp 230 15 a a LS cord-328082-7c7slfbp 230 16 ) ) -RRB- cord-328082-7c7slfbp 230 17 and and CC cord-328082-7c7slfbp 230 18 man man NN cord-328082-7c7slfbp 230 19 II II NNP cord-328082-7c7slfbp 230 20 ( ( -LRB- cord-328082-7c7slfbp 230 21 Fig Fig NNP cord-328082-7c7slfbp 230 22 . . . cord-328082-7c7slfbp 230 23 5 5 CD cord-328082-7c7slfbp 230 24 b b LS cord-328082-7c7slfbp 230 25 ) ) -RRB- cord-328082-7c7slfbp 230 26 oc oc JJ cord-328082-7c7slfbp 230 27 - - HYPH cord-328082-7c7slfbp 230 28 curred curred JJ cord-328082-7c7slfbp 230 29 only only RB cord-328082-7c7slfbp 230 30 rarely rarely RB cord-328082-7c7slfbp 230 31 . . . cord-328082-7c7slfbp 231 1 Even even RB cord-328082-7c7slfbp 231 2 though though IN cord-328082-7c7slfbp 231 3 the the DT cord-328082-7c7slfbp 231 4 majority majority NN cord-328082-7c7slfbp 231 5 of of IN cord-328082-7c7slfbp 231 6 the the DT cord-328082-7c7slfbp 231 7 p58 p58 NNP cord-328082-7c7slfbp 231 8 protein protein NN cord-328082-7c7slfbp 231 9 seemed seem VBD cord-328082-7c7slfbp 231 10 to to TO cord-328082-7c7slfbp 231 11 move move VB cord-328082-7c7slfbp 231 12 to to IN cord-328082-7c7slfbp 231 13 the the DT cord-328082-7c7slfbp 231 14 periphery periphery NN cord-328082-7c7slfbp 231 15 of of IN cord-328082-7c7slfbp 231 16 the the DT cord-328082-7c7slfbp 231 17 cell cell NN cord-328082-7c7slfbp 231 18 in in IN cord-328082-7c7slfbp 231 19 the the DT cord-328082-7c7slfbp 231 20 presence presence NN cord-328082-7c7slfbp 231 21 of of IN cord-328082-7c7slfbp 231 22 caffeine caffeine NN cord-328082-7c7slfbp 231 23 at at IN cord-328082-7c7slfbp 231 24 20~ 20~ CD cord-328082-7c7slfbp 232 1 some some DT cord-328082-7c7slfbp 232 2 p58-positive p58-positive JJ cord-328082-7c7slfbp 232 3 vesicles vesicle NNS cord-328082-7c7slfbp 232 4 still still RB cord-328082-7c7slfbp 232 5 colocalized colocalize VBN cord-328082-7c7slfbp 232 6 with with IN cord-328082-7c7slfbp 232 7 the the DT cord-328082-7c7slfbp 232 8 perinuclear perinuclear JJ cord-328082-7c7slfbp 232 9 elements element NNS cord-328082-7c7slfbp 232 10 also also RB cord-328082-7c7slfbp 232 11 labeled label VBN cord-328082-7c7slfbp 232 12 with with IN cord-328082-7c7slfbp 232 13 antibodies antibody NNS cord-328082-7c7slfbp 232 14 to to TO cord-328082-7c7slfbp 232 15 man man VB cord-328082-7c7slfbp 232 16 II II NNP cord-328082-7c7slfbp 232 17 . . . cord-328082-7c7slfbp 233 1 This this DT cord-328082-7c7slfbp 233 2 could could MD cord-328082-7c7slfbp 233 3 partly partly RB cord-328082-7c7slfbp 233 4 be be VB cord-328082-7c7slfbp 233 5 due due JJ cord-328082-7c7slfbp 233 6 to to IN cord-328082-7c7slfbp 233 7 an an DT cord-328082-7c7slfbp 233 8 apparent apparent JJ cord-328082-7c7slfbp 233 9 colocalization colocalization NN cord-328082-7c7slfbp 233 10 of of IN cord-328082-7c7slfbp 233 11 not not RB cord-328082-7c7slfbp 233 12 the the DT cord-328082-7c7slfbp 233 13 same same JJ cord-328082-7c7slfbp 233 14 but but CC cord-328082-7c7slfbp 233 15 closely closely RB cord-328082-7c7slfbp 233 16 adjacent adjacent JJ cord-328082-7c7slfbp 233 17 structures structure NNS cord-328082-7c7slfbp 233 18 . . . cord-328082-7c7slfbp 234 1 These these DT cord-328082-7c7slfbp 234 2 results result NNS cord-328082-7c7slfbp 234 3 suggest suggest VBP cord-328082-7c7slfbp 234 4 that that IN cord-328082-7c7slfbp 234 5 10 10 CD cord-328082-7c7slfbp 234 6 mM mm CD cord-328082-7c7slfbp 234 7 caffeine caffeine NN cord-328082-7c7slfbp 234 8 at at IN cord-328082-7c7slfbp 234 9 20~ 20~ CD cord-328082-7c7slfbp 234 10 induces induce VBZ cord-328082-7c7slfbp 234 11 a a DT cord-328082-7c7slfbp 234 12 translocation translocation NN cord-328082-7c7slfbp 234 13 of of IN cord-328082-7c7slfbp 234 14 p58-positive p58-positive JJ cord-328082-7c7slfbp 234 15 , , , cord-328082-7c7slfbp 234 16 pre pre JJ cord-328082-7c7slfbp 234 17 - - JJ cord-328082-7c7slfbp 234 18 Golgi Golgi NNP cord-328082-7c7slfbp 234 19 , , , cord-328082-7c7slfbp 234 20 and and CC cord-328082-7c7slfbp 234 21 cis cis NNP cord-328082-7c7slfbp 234 22 - - HYPH cord-328082-7c7slfbp 234 23 Golgi Golgi NNP cord-328082-7c7slfbp 234 24 membranes membrane NNS cord-328082-7c7slfbp 234 25 to to IN cord-328082-7c7slfbp 234 26 the the DT cord-328082-7c7slfbp 234 27 periphery periphery NN cord-328082-7c7slfbp 234 28 of of IN cord-328082-7c7slfbp 234 29 the the DT cord-328082-7c7slfbp 234 30 cells cell NNS cord-328082-7c7slfbp 234 31 leaving leave VBG cord-328082-7c7slfbp 234 32 man man NN cord-328082-7c7slfbp 234 33 II ii NN cord-328082-7c7slfbp 234 34 - - HYPH cord-328082-7c7slfbp 234 35 positive positive JJ cord-328082-7c7slfbp 234 36 membranes membrane NNS cord-328082-7c7slfbp 234 37 perinuclearly perinuclearly RB cord-328082-7c7slfbp 234 38 located locate VBN cord-328082-7c7slfbp 234 39 . . . cord-328082-7c7slfbp 235 1 To to TO cord-328082-7c7slfbp 235 2 study study VB cord-328082-7c7slfbp 235 3 the the DT cord-328082-7c7slfbp 235 4 reversibility reversibility NN cord-328082-7c7slfbp 235 5 of of IN cord-328082-7c7slfbp 235 6 the the DT cord-328082-7c7slfbp 235 7 caffeine caffeine NN cord-328082-7c7slfbp 235 8 - - HYPH cord-328082-7c7slfbp 235 9 induced induce VBN cord-328082-7c7slfbp 235 10 translocation translocation NN cord-328082-7c7slfbp 235 11 of of IN cord-328082-7c7slfbp 235 12 p58 p58 NN cord-328082-7c7slfbp 235 13 , , , cord-328082-7c7slfbp 235 14 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 235 15 cells cell NNS cord-328082-7c7slfbp 235 16 were be VBD cord-328082-7c7slfbp 235 17 treated treat VBN cord-328082-7c7slfbp 235 18 first first RB cord-328082-7c7slfbp 235 19 with with IN cord-328082-7c7slfbp 235 20 10 10 CD cord-328082-7c7slfbp 235 21 mM mm CD cord-328082-7c7slfbp 235 22 caffeine caffeine NN cord-328082-7c7slfbp 235 23 at at IN cord-328082-7c7slfbp 235 24 20~ 20~ CD cord-328082-7c7slfbp 235 25 for for IN cord-328082-7c7slfbp 235 26 60 60 CD cord-328082-7c7slfbp 235 27 min min NN cord-328082-7c7slfbp 235 28 , , , cord-328082-7c7slfbp 235 29 followed follow VBN cord-328082-7c7slfbp 235 30 by by IN cord-328082-7c7slfbp 235 31 several several JJ cord-328082-7c7slfbp 235 32 washes wash NNS cord-328082-7c7slfbp 235 33 with with IN cord-328082-7c7slfbp 235 34 noncaffeine noncaffeine JJ cord-328082-7c7slfbp 235 35 medium medium NN cord-328082-7c7slfbp 235 36 . . . cord-328082-7c7slfbp 236 1 After after IN cord-328082-7c7slfbp 236 2 incubation incubation NN cord-328082-7c7slfbp 236 3 for for IN cord-328082-7c7slfbp 236 4 an an DT cord-328082-7c7slfbp 236 5 additional additional JJ cord-328082-7c7slfbp 236 6 60 60 CD cord-328082-7c7slfbp 236 7 min min NN cord-328082-7c7slfbp 236 8 in in IN cord-328082-7c7slfbp 236 9 the the DT cord-328082-7c7slfbp 236 10 absence absence NN cord-328082-7c7slfbp 236 11 of of IN cord-328082-7c7slfbp 236 12 caffeine caffeine NN cord-328082-7c7slfbp 236 13 at at IN cord-328082-7c7slfbp 236 14 20~ 20~ CD cord-328082-7c7slfbp 236 15 the the DT cord-328082-7c7slfbp 236 16 localization localization NN cord-328082-7c7slfbp 236 17 of of IN cord-328082-7c7slfbp 236 18 p58 p58 NNP cord-328082-7c7slfbp 236 19 was be VBD cord-328082-7c7slfbp 236 20 compared compare VBN cord-328082-7c7slfbp 236 21 with with IN cord-328082-7c7slfbp 236 22 that that DT cord-328082-7c7slfbp 236 23 at at IN cord-328082-7c7slfbp 236 24 normal normal JJ cord-328082-7c7slfbp 236 25 temperature temperature NN cord-328082-7c7slfbp 236 26 ( ( -LRB- cord-328082-7c7slfbp 236 27 37~ 37~ CD cord-328082-7c7slfbp 236 28 and and CC cord-328082-7c7slfbp 236 29 to to IN cord-328082-7c7slfbp 236 30 that that DT cord-328082-7c7slfbp 236 31 at at IN cord-328082-7c7slfbp 236 32 20~ 20~ CD cord-328082-7c7slfbp 236 33 without without IN cord-328082-7c7slfbp 236 34 caffeine caffeine NN cord-328082-7c7slfbp 236 35 . . . cord-328082-7c7slfbp 237 1 In in IN cord-328082-7c7slfbp 237 2 addition addition NN cord-328082-7c7slfbp 237 3 , , , cord-328082-7c7slfbp 237 4 the the DT cord-328082-7c7slfbp 237 5 localization localization NN cord-328082-7c7slfbp 237 6 of of IN cord-328082-7c7slfbp 237 7 p58 p58 NNP cord-328082-7c7slfbp 237 8 was be VBD cord-328082-7c7slfbp 237 9 compared compare VBN cord-328082-7c7slfbp 237 10 with with IN cord-328082-7c7slfbp 237 11 that that DT cord-328082-7c7slfbp 237 12 of of IN cord-328082-7c7slfbp 237 13 man man NN cord-328082-7c7slfbp 237 14 II II NNP cord-328082-7c7slfbp 237 15 in in IN cord-328082-7c7slfbp 237 16 the the DT cord-328082-7c7slfbp 237 17 same same JJ cord-328082-7c7slfbp 237 18 cells cell NNS cord-328082-7c7slfbp 237 19 . . . cord-328082-7c7slfbp 238 1 In in IN cord-328082-7c7slfbp 238 2 untreated untreated JJ cord-328082-7c7slfbp 238 3 cells cell NNS cord-328082-7c7slfbp 238 4 , , , cord-328082-7c7slfbp 238 5 p58 p58 NNP cord-328082-7c7slfbp 238 6 is be VBZ cord-328082-7c7slfbp 238 7 located locate VBN cord-328082-7c7slfbp 238 8 perinuclearly perinuclearly RB cord-328082-7c7slfbp 238 9 with with IN cord-328082-7c7slfbp 238 10 some some DT cord-328082-7c7slfbp 238 11 labeling labeling NN cord-328082-7c7slfbp 238 12 in in IN cord-328082-7c7slfbp 238 13 small small JJ cord-328082-7c7slfbp 238 14 peripheral peripheral JJ cord-328082-7c7slfbp 238 15 vesicles vesicle NNS cord-328082-7c7slfbp 238 16 and and CC cord-328082-7c7slfbp 238 17 ER ER NNP cord-328082-7c7slfbp 238 18 as as IN cord-328082-7c7slfbp 238 19 reported report VBN cord-328082-7c7slfbp 238 20 earlier early RBR cord-328082-7c7slfbp 238 21 ( ( -LRB- cord-328082-7c7slfbp 238 22 Saraste Saraste NNP cord-328082-7c7slfbp 238 23 and and CC cord-328082-7c7slfbp 238 24 Svensson Svensson NNP cord-328082-7c7slfbp 238 25 , , , cord-328082-7c7slfbp 238 26 1991 1991 CD cord-328082-7c7slfbp 238 27 ) ) -RRB- cord-328082-7c7slfbp 239 1 ( ( -LRB- cord-328082-7c7slfbp 239 2 Fig Fig NNP cord-328082-7c7slfbp 239 3 . . NNP cord-328082-7c7slfbp 239 4 6 6 CD cord-328082-7c7slfbp 239 5 , , , cord-328082-7c7slfbp 239 6 no no DT cord-328082-7c7slfbp 239 7 drug drug NN cord-328082-7c7slfbp 239 8 ) ) -RRB- cord-328082-7c7slfbp 239 9 . . . cord-328082-7c7slfbp 240 1 In in IN cord-328082-7c7slfbp 240 2 cells cell NNS cord-328082-7c7slfbp 240 3 treated treat VBN cord-328082-7c7slfbp 240 4 with with IN cord-328082-7c7slfbp 240 5 caffeine caffeine NN cord-328082-7c7slfbp 240 6 at at IN cord-328082-7c7slfbp 240 7 20~ 20~ CD cord-328082-7c7slfbp 240 8 for for IN cord-328082-7c7slfbp 240 9 60 60 CD cord-328082-7c7slfbp 240 10 min min NN cord-328082-7c7slfbp 240 11 ( ( -LRB- cord-328082-7c7slfbp 240 12 Fig Fig NNP cord-328082-7c7slfbp 240 13 . . NNP cord-328082-7c7slfbp 240 14 6 6 CD cord-328082-7c7slfbp 240 15 , , , cord-328082-7c7slfbp 240 16 20~ 20~ CD cord-328082-7c7slfbp 240 17 caffeine caffeine NN cord-328082-7c7slfbp 240 18 ) ) -RRB- cord-328082-7c7slfbp 240 19 , , , cord-328082-7c7slfbp 240 20 the the DT cord-328082-7c7slfbp 240 21 bulk bulk NN cord-328082-7c7slfbp 240 22 of of IN cord-328082-7c7slfbp 240 23 p58 p58 NNP cord-328082-7c7slfbp 240 24 becomes become VBZ cord-328082-7c7slfbp 240 25 distributed distribute VBN cord-328082-7c7slfbp 240 26 throughout throughout IN cord-328082-7c7slfbp 240 27 the the DT cord-328082-7c7slfbp 240 28 cytoplasm cytoplasm NN cord-328082-7c7slfbp 240 29 while while IN cord-328082-7c7slfbp 240 30 man man NN cord-328082-7c7slfbp 240 31 II II NNP cord-328082-7c7slfbp 240 32 still still RB cord-328082-7c7slfbp 240 33 remains remain VBZ cord-328082-7c7slfbp 240 34 perinuclearly perinuclearly RB cord-328082-7c7slfbp 240 35 located locate VBN cord-328082-7c7slfbp 240 36 . . . cord-328082-7c7slfbp 241 1 On on IN cord-328082-7c7slfbp 241 2 the the DT cord-328082-7c7slfbp 241 3 contrary contrary NN cord-328082-7c7slfbp 241 4 , , , cord-328082-7c7slfbp 241 5 in in IN cord-328082-7c7slfbp 241 6 control control NN cord-328082-7c7slfbp 241 7 cells cell NNS cord-328082-7c7slfbp 241 8 without without IN cord-328082-7c7slfbp 241 9 caffeine caffeine NN cord-328082-7c7slfbp 241 10 at at IN cord-328082-7c7slfbp 241 11 20~ 20~ CD cord-328082-7c7slfbp 241 12 p58 p58 NN cord-328082-7c7slfbp 241 13 was be VBD cord-328082-7c7slfbp 241 14 concentrated concentrate VBN cord-328082-7c7slfbp 241 15 to to IN cord-328082-7c7slfbp 241 16 the the DT cord-328082-7c7slfbp 241 17 Golgi Golgi NNP cord-328082-7c7slfbp 241 18 area area NN cord-328082-7c7slfbp 241 19 and and CC cord-328082-7c7slfbp 241 20 colocalized colocalize VBN cord-328082-7c7slfbp 241 21 with with IN cord-328082-7c7slfbp 241 22 man man NN cord-328082-7c7slfbp 241 23 II II NNP cord-328082-7c7slfbp 242 1 ( ( -LRB- cord-328082-7c7slfbp 242 2 Fig Fig NNP cord-328082-7c7slfbp 242 3 . . NNP cord-328082-7c7slfbp 242 4 6 6 CD cord-328082-7c7slfbp 242 5 , , , cord-328082-7c7slfbp 242 6 20~ 20~ CD cord-328082-7c7slfbp 242 7 control control NN cord-328082-7c7slfbp 242 8 ) ) -RRB- cord-328082-7c7slfbp 242 9 . . . cord-328082-7c7slfbp 243 1 The the DT cord-328082-7c7slfbp 243 2 normal normal JJ cord-328082-7c7slfbp 243 3 localization localization NN cord-328082-7c7slfbp 243 4 of of IN cord-328082-7c7slfbp 243 5 p58 p58 NN cord-328082-7c7slfbp 243 6 was be VBD cord-328082-7c7slfbp 243 7 restored restore VBN cord-328082-7c7slfbp 243 8 when when WRB cord-328082-7c7slfbp 243 9 the the DT cord-328082-7c7slfbp 243 10 cells cell NNS cord-328082-7c7slfbp 243 11 maintained maintain VBD cord-328082-7c7slfbp 243 12 for for IN cord-328082-7c7slfbp 243 13 60 60 CD cord-328082-7c7slfbp 243 14 min min NN cord-328082-7c7slfbp 243 15 in in IN cord-328082-7c7slfbp 243 16 the the DT cord-328082-7c7slfbp 243 17 presence presence NN cord-328082-7c7slfbp 243 18 of of IN cord-328082-7c7slfbp 243 19 10 10 CD cord-328082-7c7slfbp 243 20 mM mm CD cord-328082-7c7slfbp 243 21 caffeine caffeine NN cord-328082-7c7slfbp 243 22 at at IN cord-328082-7c7slfbp 243 23 20~ 20~ CD cord-328082-7c7slfbp 243 24 were be VBD cord-328082-7c7slfbp 243 25 further further RB cord-328082-7c7slfbp 243 26 incubated incubate VBN cord-328082-7c7slfbp 243 27 for for IN cord-328082-7c7slfbp 243 28 60 60 CD cord-328082-7c7slfbp 243 29 min min NN cord-328082-7c7slfbp 243 30 at at IN cord-328082-7c7slfbp 243 31 20~ 20~ CD cord-328082-7c7slfbp 243 32 in in IN cord-328082-7c7slfbp 243 33 a a DT cord-328082-7c7slfbp 243 34 caffeine caffeine NN cord-328082-7c7slfbp 243 35 - - HYPH cord-328082-7c7slfbp 243 36 free free JJ cord-328082-7c7slfbp 243 37 medium medium NN cord-328082-7c7slfbp 243 38 ( ( -LRB- cord-328082-7c7slfbp 243 39 Fig fig NN cord-328082-7c7slfbp 243 40 . . . cord-328082-7c7slfbp 243 41 _SP cord-328082-7c7slfbp 243 42 6 6 CD cord-328082-7c7slfbp 243 43 , , , cord-328082-7c7slfbp 243 44 reversion reversion NN cord-328082-7c7slfbp 243 45 ) ) -RRB- cord-328082-7c7slfbp 243 46 . . . cord-328082-7c7slfbp 244 1 The the DT cord-328082-7c7slfbp 244 2 observed observed JJ cord-328082-7c7slfbp 244 3 reversible reversible JJ cord-328082-7c7slfbp 244 4 translocation translocation NN cord-328082-7c7slfbp 244 5 of of IN cord-328082-7c7slfbp 244 6 preand preand NNP cord-328082-7c7slfbp 244 7 cis cis NNP cord-328082-7c7slfbp 244 8 - - HYPH cord-328082-7c7slfbp 244 9 Golgi Golgi NNP cord-328082-7c7slfbp 244 10 marker marker NN cord-328082-7c7slfbp 244 11 protein protein NN cord-328082-7c7slfbp 244 12 to to IN cord-328082-7c7slfbp 244 13 the the DT cord-328082-7c7slfbp 244 14 periphery periphery NN cord-328082-7c7slfbp 244 15 of of IN cord-328082-7c7slfbp 244 16 the the DT cord-328082-7c7slfbp 244 17 cell cell NN cord-328082-7c7slfbp 244 18 and and CC cord-328082-7c7slfbp 244 19 the the DT cord-328082-7c7slfbp 244 20 unaltered unaltered JJ cord-328082-7c7slfbp 244 21 distribution distribution NN cord-328082-7c7slfbp 244 22 of of IN cord-328082-7c7slfbp 244 23 a a DT cord-328082-7c7slfbp 244 24 Golgi Golgi NNP cord-328082-7c7slfbp 244 25 stack stack NN cord-328082-7c7slfbp 244 26 marker marker NN cord-328082-7c7slfbp 244 27 protein protein NN cord-328082-7c7slfbp 244 28 suggest suggest VBP cord-328082-7c7slfbp 244 29 that that IN cord-328082-7c7slfbp 244 30 caffeine caffeine NN cord-328082-7c7slfbp 244 31 at at IN cord-328082-7c7slfbp 244 32 reduced reduce VBN cord-328082-7c7slfbp 244 33 temperature temperature NN cord-328082-7c7slfbp 244 34 can can MD cord-328082-7c7slfbp 244 35 be be VB cord-328082-7c7slfbp 244 36 used use VBN cord-328082-7c7slfbp 244 37 to to TO cord-328082-7c7slfbp 244 38 manipulate manipulate VB cord-328082-7c7slfbp 244 39 pre pre JJ cord-328082-7c7slfbp 244 40 - - JJ cord-328082-7c7slfbp 244 41 and and CC cord-328082-7c7slfbp 244 42 cis cis NNP cord-328082-7c7slfbp 244 43 - - HYPH cord-328082-7c7slfbp 244 44 Golgi Golgi NNP cord-328082-7c7slfbp 244 45 elements element NNS cord-328082-7c7slfbp 244 46 without without IN cord-328082-7c7slfbp 244 47 affecting affect VBG cord-328082-7c7slfbp 244 48 the the DT cord-328082-7c7slfbp 244 49 localization localization NN cord-328082-7c7slfbp 244 50 of of IN cord-328082-7c7slfbp 244 51 Golgi Golgi NNP cord-328082-7c7slfbp 244 52 stack stack NN cord-328082-7c7slfbp 244 53 membranes membrane NNS cord-328082-7c7slfbp 244 54 . . . cord-328082-7c7slfbp 245 1 Because because IN cord-328082-7c7slfbp 245 2 of of IN cord-328082-7c7slfbp 245 3 the the DT cord-328082-7c7slfbp 245 4 effective effective JJ cord-328082-7c7slfbp 245 5 inhibition inhibition NN cord-328082-7c7slfbp 245 6 of of IN cord-328082-7c7slfbp 245 7 membrane membrane NN cord-328082-7c7slfbp 245 8 traffic traffic NN cord-328082-7c7slfbp 245 9 out out IN cord-328082-7c7slfbp 245 10 of of IN cord-328082-7c7slfbp 245 11 the the DT cord-328082-7c7slfbp 245 12 ER ER NNP cord-328082-7c7slfbp 245 13 at at IN cord-328082-7c7slfbp 245 14 20~ 20~ CD cord-328082-7c7slfbp 245 15 and and CC cord-328082-7c7slfbp 245 16 the the DT cord-328082-7c7slfbp 245 17 translocation translocation NN cord-328082-7c7slfbp 245 18 of of IN cord-328082-7c7slfbp 245 19 p58 p58 NN cord-328082-7c7slfbp 245 20 to to IN cord-328082-7c7slfbp 245 21 the the DT cord-328082-7c7slfbp 245 22 periphery periphery NN cord-328082-7c7slfbp 245 23 of of IN cord-328082-7c7slfbp 245 24 the the DT cord-328082-7c7slfbp 245 25 cell cell NN cord-328082-7c7slfbp 245 26 in in IN cord-328082-7c7slfbp 245 27 the the DT cord-328082-7c7slfbp 245 28 presence presence NN cord-328082-7c7slfbp 245 29 of of IN cord-328082-7c7slfbp 245 30 caffeine caffeine NN cord-328082-7c7slfbp 245 31 , , , cord-328082-7c7slfbp 245 32 it -PRON- PRP cord-328082-7c7slfbp 245 33 was be VBD cord-328082-7c7slfbp 245 34 of of IN cord-328082-7c7slfbp 245 35 great great JJ cord-328082-7c7slfbp 245 36 interest interest NN cord-328082-7c7slfbp 245 37 to to TO cord-328082-7c7slfbp 245 38 study study VB cord-328082-7c7slfbp 245 39 whether whether IN cord-328082-7c7slfbp 245 40 the the DT cord-328082-7c7slfbp 245 41 morphology morphology NN cord-328082-7c7slfbp 245 42 of of IN cord-328082-7c7slfbp 245 43 the the DT cord-328082-7c7slfbp 245 44 Golgi Golgi NNP cord-328082-7c7slfbp 245 45 stack stack NN cord-328082-7c7slfbp 245 46 is be VBZ cord-328082-7c7slfbp 245 47 affected affect VBN cord-328082-7c7slfbp 245 48 under under IN cord-328082-7c7slfbp 245 49 the the DT cord-328082-7c7slfbp 245 50 conditions condition NNS cord-328082-7c7slfbp 245 51 used use VBN cord-328082-7c7slfbp 245 52 . . . cord-328082-7c7slfbp 246 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 247 1 cells cell NNS cord-328082-7c7slfbp 247 2 were be VBD cord-328082-7c7slfbp 247 3 incubated incubate VBN cord-328082-7c7slfbp 247 4 for for IN cord-328082-7c7slfbp 247 5 120 120 CD cord-328082-7c7slfbp 247 6 rain rain NN cord-328082-7c7slfbp 247 7 in in IN cord-328082-7c7slfbp 247 8 the the DT cord-328082-7c7slfbp 247 9 presence presence NN cord-328082-7c7slfbp 247 10 or or CC cord-328082-7c7slfbp 247 11 absence absence NN cord-328082-7c7slfbp 247 12 of of IN cord-328082-7c7slfbp 247 13 10 10 CD cord-328082-7c7slfbp 247 14 mM mm CD cord-328082-7c7slfbp 247 15 caffeine caffeine NN cord-328082-7c7slfbp 247 16 at at IN cord-328082-7c7slfbp 247 17 20~ 20~ CD cord-328082-7c7slfbp 247 18 fixed fix VBN cord-328082-7c7slfbp 247 19 , , , cord-328082-7c7slfbp 247 20 and and CC cord-328082-7c7slfbp 247 21 processed process VBN cord-328082-7c7slfbp 247 22 for for IN cord-328082-7c7slfbp 247 23 electron electron NN cord-328082-7c7slfbp 247 24 microscopy microscopy NN cord-328082-7c7slfbp 247 25 . . . cord-328082-7c7slfbp 248 1 In in IN cord-328082-7c7slfbp 248 2 caffeine caffeine NN cord-328082-7c7slfbp 248 3 - - HYPH cord-328082-7c7slfbp 248 4 treated treat VBN cord-328082-7c7slfbp 248 5 cells cell NNS cord-328082-7c7slfbp 248 6 the the DT cord-328082-7c7slfbp 248 7 morphology morphology NN cord-328082-7c7slfbp 248 8 of of IN cord-328082-7c7slfbp 248 9 the the DT cord-328082-7c7slfbp 248 10 Golgi Golgi NNP cord-328082-7c7slfbp 248 11 stack stack NN cord-328082-7c7slfbp 248 12 was be VBD cord-328082-7c7slfbp 248 13 altered alter VBN cord-328082-7c7slfbp 248 14 . . . cord-328082-7c7slfbp 249 1 The the DT cord-328082-7c7slfbp 249 2 Golgi Golgi NNP cord-328082-7c7slfbp 249 3 regions region NNS cord-328082-7c7slfbp 249 4 of of IN cord-328082-7c7slfbp 249 5 the the DT cord-328082-7c7slfbp 249 6 cells cell NNS cord-328082-7c7slfbp 249 7 were be VBD cord-328082-7c7slfbp 249 8 filled fill VBN cord-328082-7c7slfbp 249 9 with with IN cord-328082-7c7slfbp 249 10 small small JJ cord-328082-7c7slfbp 249 11 ( ( -LRB- cord-328082-7c7slfbp 249 12 60 60 CD cord-328082-7c7slfbp 249 13 - - HYPH cord-328082-7c7slfbp 249 14 70-nm 70-nm CD cord-328082-7c7slfbp 249 15 - - HYPH cord-328082-7c7slfbp 249 16 diam diam NNP cord-328082-7c7slfbp 249 17 ) ) -RRB- cord-328082-7c7slfbp 249 18 vesicles vesicle NNS cord-328082-7c7slfbp 249 19 ( ( -LRB- cord-328082-7c7slfbp 249 20 Fig Fig NNP cord-328082-7c7slfbp 249 21 . . . cord-328082-7c7slfbp 249 22 7 7 CD cord-328082-7c7slfbp 249 23 a a DT cord-328082-7c7slfbp 249 24 ) ) -RRB- cord-328082-7c7slfbp 249 25 . . . cord-328082-7c7slfbp 250 1 In in IN cord-328082-7c7slfbp 250 2 addition addition NN cord-328082-7c7slfbp 250 3 , , , cord-328082-7c7slfbp 250 4 some some DT cord-328082-7c7slfbp 250 5 tubular tubular JJ cord-328082-7c7slfbp 250 6 membranous membranous JJ cord-328082-7c7slfbp 250 7 structures structure NNS cord-328082-7c7slfbp 250 8 , , , cord-328082-7c7slfbp 250 9 possibly possibly RB cord-328082-7c7slfbp 250 10 representing represent VBG cord-328082-7c7slfbp 250 11 remnants remnant NNS cord-328082-7c7slfbp 250 12 of of IN cord-328082-7c7slfbp 250 13 the the DT cord-328082-7c7slfbp 250 14 Golgi Golgi NNP cord-328082-7c7slfbp 250 15 stack stack NN cord-328082-7c7slfbp 250 16 , , , cord-328082-7c7slfbp 250 17 were be VBD cord-328082-7c7slfbp 250 18 also also RB cord-328082-7c7slfbp 250 19 observed observe VBN cord-328082-7c7slfbp 250 20 ( ( -LRB- cord-328082-7c7slfbp 250 21 Fig Fig NNP cord-328082-7c7slfbp 250 22 . . . cord-328082-7c7slfbp 250 23 7 7 CD cord-328082-7c7slfbp 250 24 a a DT cord-328082-7c7slfbp 250 25 , , , cord-328082-7c7slfbp 250 26 _SP cord-328082-7c7slfbp 250 27 arrow arrow NN cord-328082-7c7slfbp 250 28 ) ) -RRB- cord-328082-7c7slfbp 250 29 . . . cord-328082-7c7slfbp 251 1 On on IN cord-328082-7c7slfbp 251 2 the the DT cord-328082-7c7slfbp 251 3 contrary contrary NN cord-328082-7c7slfbp 251 4 , , , cord-328082-7c7slfbp 251 5 control control NN cord-328082-7c7slfbp 251 6 cells cell NNS cord-328082-7c7slfbp 251 7 incubated incubate VBN cord-328082-7c7slfbp 251 8 for for IN cord-328082-7c7slfbp 251 9 120 120 CD cord-328082-7c7slfbp 251 10 min min NN cord-328082-7c7slfbp 251 11 at at IN cord-328082-7c7slfbp 251 12 20~ 20~ CD cord-328082-7c7slfbp 251 13 possessed possess VBD cord-328082-7c7slfbp 251 14 typical typical JJ cord-328082-7c7slfbp 251 15 Golgi Golgi NNP cord-328082-7c7slfbp 251 16 stacks stack NNS cord-328082-7c7slfbp 252 1 ( ( -LRB- cord-328082-7c7slfbp 252 2 Fig Fig NNP cord-328082-7c7slfbp 252 3 . . . cord-328082-7c7slfbp 253 1 7 7 CD cord-328082-7c7slfbp 253 2 b b NN cord-328082-7c7slfbp 253 3 ) ) -RRB- cord-328082-7c7slfbp 253 4 . . . cord-328082-7c7slfbp 254 1 Taken take VBN cord-328082-7c7slfbp 254 2 together together RB cord-328082-7c7slfbp 254 3 , , , cord-328082-7c7slfbp 254 4 caffeine caffeine NN cord-328082-7c7slfbp 254 5 at at IN cord-328082-7c7slfbp 254 6 20~ 20~ CD cord-328082-7c7slfbp 254 7 does do VBZ cord-328082-7c7slfbp 254 8 not not RB cord-328082-7c7slfbp 254 9 only only RB cord-328082-7c7slfbp 254 10 inhibit inhibit VB cord-328082-7c7slfbp 254 11 the the DT cord-328082-7c7slfbp 254 12 ER er NN cord-328082-7c7slfbp 254 13 - - HYPH cord-328082-7c7slfbp 254 14 exit exit NN cord-328082-7c7slfbp 254 15 of of IN cord-328082-7c7slfbp 254 16 proteins protein NNS cord-328082-7c7slfbp 254 17 and and CC cord-328082-7c7slfbp 254 18 translocate translocate VBP cord-328082-7c7slfbp 254 19 p58 p58 PRP cord-328082-7c7slfbp 254 20 to to IN cord-328082-7c7slfbp 254 21 the the DT cord-328082-7c7slfbp 254 22 periphery periphery NN cord-328082-7c7slfbp 254 23 of of IN cord-328082-7c7slfbp 254 24 the the DT cord-328082-7c7slfbp 254 25 cells cell NNS cord-328082-7c7slfbp 254 26 but but CC cord-328082-7c7slfbp 254 27 also also RB cord-328082-7c7slfbp 254 28 induces induce VBZ cord-328082-7c7slfbp 254 29 a a DT cord-328082-7c7slfbp 254 30 change change NN cord-328082-7c7slfbp 254 31 in in IN cord-328082-7c7slfbp 254 32 the the DT cord-328082-7c7slfbp 254 33 morphology morphology NN cord-328082-7c7slfbp 254 34 of of IN cord-328082-7c7slfbp 254 35 the the DT cord-328082-7c7slfbp 254 36 Golgi Golgi NNP cord-328082-7c7slfbp 254 37 stack stack NN cord-328082-7c7slfbp 254 38 . . . cord-328082-7c7slfbp 255 1 To to TO cord-328082-7c7slfbp 255 2 study study VB cord-328082-7c7slfbp 255 3 the the DT cord-328082-7c7slfbp 255 4 distribution distribution NN cord-328082-7c7slfbp 255 5 of of IN cord-328082-7c7slfbp 255 6 p58 p58 NN cord-328082-7c7slfbp 255 7 in in IN cord-328082-7c7slfbp 255 8 the the DT cord-328082-7c7slfbp 255 9 morphologically morphologically RB cord-328082-7c7slfbp 255 10 altered alter VBN cord-328082-7c7slfbp 255 11 Golgi Golgi NNP cord-328082-7c7slfbp 255 12 region region NN cord-328082-7c7slfbp 255 13 in in IN cord-328082-7c7slfbp 255 14 caffeine caffeine NN cord-328082-7c7slfbp 255 15 - - HYPH cord-328082-7c7slfbp 255 16 treated treat VBN cord-328082-7c7slfbp 255 17 ceils ceil NNS cord-328082-7c7slfbp 255 18 , , , cord-328082-7c7slfbp 255 19 we -PRON- PRP cord-328082-7c7slfbp 255 20 performed perform VBD cord-328082-7c7slfbp 255 21 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 255 22 microscopy microscopy NN cord-328082-7c7slfbp 255 23 experiments experiment NNS cord-328082-7c7slfbp 255 24 . . . cord-328082-7c7slfbp 256 1 In in IN cord-328082-7c7slfbp 256 2 control control NN cord-328082-7c7slfbp 256 3 cells cell NNS cord-328082-7c7slfbp 256 4 , , , cord-328082-7c7slfbp 256 5 maintained maintain VBD cord-328082-7c7slfbp 256 6 at at IN cord-328082-7c7slfbp 256 7 20~ 20~ CD cord-328082-7c7slfbp 256 8 for for IN cord-328082-7c7slfbp 256 9 60 60 CD cord-328082-7c7slfbp 256 10 min min NN cord-328082-7c7slfbp 256 11 , , , cord-328082-7c7slfbp 256 12 p58 p58 NNP cord-328082-7c7slfbp 256 13 was be VBD cord-328082-7c7slfbp 256 14 found find VBN cord-328082-7c7slfbp 256 15 in in IN cord-328082-7c7slfbp 256 16 cis cis NNP cord-328082-7c7slfbp 256 17 - - HYPH cord-328082-7c7slfbp 256 18 Golgi Golgi NNP cord-328082-7c7slfbp 256 19 and and CC cord-328082-7c7slfbp 256 20 also also RB cord-328082-7c7slfbp 256 21 in in IN cord-328082-7c7slfbp 256 22 cytoplasmic cytoplasmic JJ cord-328082-7c7slfbp 256 23 tubulovesicular tubulovesicular JJ cord-328082-7c7slfbp 256 24 elements element NNS cord-328082-7c7slfbp 256 25 ( ( -LRB- cord-328082-7c7slfbp 256 26 Saraste Saraste NNP cord-328082-7c7slfbp 256 27 et et NNP cord-328082-7c7slfbp 256 28 al al NNP cord-328082-7c7slfbp 256 29 . . NNP cord-328082-7c7slfbp 256 30 , , , cord-328082-7c7slfbp 256 31 1987 1987 CD cord-328082-7c7slfbp 256 32 ; ; . cord-328082-7c7slfbp 257 1 Saraste Saraste NNP cord-328082-7c7slfbp 257 2 and and CC cord-328082-7c7slfbp 257 3 Svensson Svensson NNP cord-328082-7c7slfbp 257 4 , , , cord-328082-7c7slfbp 257 5 1991 1991 CD cord-328082-7c7slfbp 257 6 ) ) -RRB- cord-328082-7c7slfbp 258 1 ( ( -LRB- cord-328082-7c7slfbp 258 2 Fig Fig NNP cord-328082-7c7slfbp 258 3 . . . cord-328082-7c7slfbp 258 4 8 8 CD cord-328082-7c7slfbp 258 5 b b NN cord-328082-7c7slfbp 258 6 , , , cord-328082-7c7slfbp 258 7 arrows arrow NNS cord-328082-7c7slfbp 258 8 ) ) -RRB- cord-328082-7c7slfbp 258 9 . . . cord-328082-7c7slfbp 259 1 When when WRB cord-328082-7c7slfbp 259 2 the the DT cord-328082-7c7slfbp 259 3 cells cell NNS cord-328082-7c7slfbp 259 4 were be VBD cord-328082-7c7slfbp 259 5 incubated incubate VBN cord-328082-7c7slfbp 259 6 for for IN cord-328082-7c7slfbp 259 7 60 60 CD cord-328082-7c7slfbp 259 8 min min NN cord-328082-7c7slfbp 259 9 at at IN cord-328082-7c7slfbp 259 10 20~ 20~ CD cord-328082-7c7slfbp 259 11 in in IN cord-328082-7c7slfbp 259 12 the the DT cord-328082-7c7slfbp 259 13 presence presence NN cord-328082-7c7slfbp 259 14 of of IN cord-328082-7c7slfbp 259 15 10 10 CD cord-328082-7c7slfbp 259 16 mM mm CD cord-328082-7c7slfbp 259 17 caffeine caffeine NN cord-328082-7c7slfbp 259 18 the the DT cord-328082-7c7slfbp 259 19 morphology morphology NN cord-328082-7c7slfbp 259 20 of of IN cord-328082-7c7slfbp 259 21 the the DT cord-328082-7c7slfbp 259 22 Golgi Golgi NNP cord-328082-7c7slfbp 259 23 stack stack NN cord-328082-7c7slfbp 259 24 was be VBD cord-328082-7c7slfbp 259 25 altered alter VBN cord-328082-7c7slfbp 259 26 as as IN cord-328082-7c7slfbp 259 27 described describe VBN cord-328082-7c7slfbp 259 28 above above IN cord-328082-7c7slfbp 259 29 ( ( -LRB- cord-328082-7c7slfbp 259 30 Fig Fig NNP cord-328082-7c7slfbp 259 31 . . . cord-328082-7c7slfbp 259 32 7 7 CD cord-328082-7c7slfbp 259 33 a a DT cord-328082-7c7slfbp 259 34 ) ) -RRB- cord-328082-7c7slfbp 259 35 . . . cord-328082-7c7slfbp 260 1 Only only RB cord-328082-7c7slfbp 260 2 a a DT cord-328082-7c7slfbp 260 3 few few JJ cord-328082-7c7slfbp 260 4 p58-positive p58-positive JJ cord-328082-7c7slfbp 260 5 vesicles vesicle NNS cord-328082-7c7slfbp 260 6 could could MD cord-328082-7c7slfbp 260 7 be be VB cord-328082-7c7slfbp 260 8 found find VBN cord-328082-7c7slfbp 260 9 in in IN cord-328082-7c7slfbp 260 10 the the DT cord-328082-7c7slfbp 260 11 Golgi Golgi NNP cord-328082-7c7slfbp 260 12 area area NN cord-328082-7c7slfbp 260 13 ( ( -LRB- cord-328082-7c7slfbp 260 14 Fig fig NN cord-328082-7c7slfbp 260 15 . . . cord-328082-7c7slfbp 260 16 _SP cord-328082-7c7slfbp 260 17 8 8 CD cord-328082-7c7slfbp 260 18 a a DT cord-328082-7c7slfbp 260 19 , , , cord-328082-7c7slfbp 260 20 arrows arrow NNS cord-328082-7c7slfbp 260 21 ) ) -RRB- cord-328082-7c7slfbp 260 22 , , , cord-328082-7c7slfbp 260 23 thus thus RB cord-328082-7c7slfbp 260 24 supporting support VBG cord-328082-7c7slfbp 260 25 the the DT cord-328082-7c7slfbp 260 26 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 260 27 finding finding NN cord-328082-7c7slfbp 260 28 where where WRB cord-328082-7c7slfbp 260 29 the the DT cord-328082-7c7slfbp 260 30 majority majority NN cord-328082-7c7slfbp 260 31 of of IN cord-328082-7c7slfbp 260 32 the the DT cord-328082-7c7slfbp 260 33 p58 p58 NNP cord-328082-7c7slfbp 260 34 seemed seem VBD cord-328082-7c7slfbp 260 35 to to TO cord-328082-7c7slfbp 260 36 get get VB cord-328082-7c7slfbp 260 37 translocated translocate VBN cord-328082-7c7slfbp 260 38 to to IN cord-328082-7c7slfbp 260 39 the the DT cord-328082-7c7slfbp 260 40 periphery periphery NN cord-328082-7c7slfbp 260 41 of of IN cord-328082-7c7slfbp 260 42 the the DT cord-328082-7c7slfbp 260 43 cells cell NNS cord-328082-7c7slfbp 260 44 while while IN cord-328082-7c7slfbp 260 45 some some DT cord-328082-7c7slfbp 260 46 of of IN cord-328082-7c7slfbp 260 47 it -PRON- PRP cord-328082-7c7slfbp 260 48 remained remain VBD cord-328082-7c7slfbp 260 49 also also RB cord-328082-7c7slfbp 260 50 located locate VBN cord-328082-7c7slfbp 260 51 near near IN cord-328082-7c7slfbp 260 52 the the DT cord-328082-7c7slfbp 260 53 nucleus nucleus NN cord-328082-7c7slfbp 260 54 . . . cord-328082-7c7slfbp 261 1 It -PRON- PRP cord-328082-7c7slfbp 261 2 has have VBZ cord-328082-7c7slfbp 261 3 been be VBN cord-328082-7c7slfbp 261 4 shown show VBN cord-328082-7c7slfbp 261 5 that that IN cord-328082-7c7slfbp 261 6 the the DT cord-328082-7c7slfbp 261 7 pre pre JJ cord-328082-7c7slfbp 261 8 - - JJ cord-328082-7c7slfbp 261 9 Golgi golgi JJ cord-328082-7c7slfbp 261 10 markers marker NNS cord-328082-7c7slfbp 261 11 p58 p58 XX cord-328082-7c7slfbp 261 12 in in IN cord-328082-7c7slfbp 261 13 normal normal JJ cord-328082-7c7slfbp 261 14 rat rat NN cord-328082-7c7slfbp 261 15 kidney kidney NN cord-328082-7c7slfbp 261 16 cells cell NNS cord-328082-7c7slfbp 261 17 ( ( -LRB- cord-328082-7c7slfbp 261 18 Saraste Saraste NNP cord-328082-7c7slfbp 261 19 and and CC cord-328082-7c7slfbp 261 20 Svensson Svensson NNP cord-328082-7c7slfbp 261 21 , , , cord-328082-7c7slfbp 261 22 1991 1991 CD cord-328082-7c7slfbp 261 23 ) ) -RRB- cord-328082-7c7slfbp 261 24 and and CC cord-328082-7c7slfbp 261 25 p53 p53 NN cord-328082-7c7slfbp 261 26 ( ( -LRB- cord-328082-7c7slfbp 261 27 Schweizer Schweizer NNP cord-328082-7c7slfbp 261 28 et et NNP cord-328082-7c7slfbp 261 29 al al NNP cord-328082-7c7slfbp 261 30 . . . cord-328082-7c7slfbp 262 1 , , , cord-328082-7c7slfbp 262 2 1988 1988 CD cord-328082-7c7slfbp 262 3 ) ) -RRB- cord-328082-7c7slfbp 262 4 in in IN cord-328082-7c7slfbp 262 5 M1 M1 NNP cord-328082-7c7slfbp 262 6 cells cell NNS cord-328082-7c7slfbp 262 7 ) ) -RRB- cord-328082-7c7slfbp 262 8 get get VBP cord-328082-7c7slfbp 262 9 translocated translocate VBN cord-328082-7c7slfbp 262 10 to to IN cord-328082-7c7slfbp 262 11 the the DT cord-328082-7c7slfbp 262 12 periphery periphery NN cord-328082-7c7slfbp 262 13 of of IN cord-328082-7c7slfbp 262 14 the the DT cord-328082-7c7slfbp 262 15 cell cell NN cord-328082-7c7slfbp 262 16 after after IN cord-328082-7c7slfbp 262 17 a a DT cord-328082-7c7slfbp 262 18 BFA BFA NNP cord-328082-7c7slfbp 262 19 treatment treatment NN cord-328082-7c7slfbp 262 20 . . . cord-328082-7c7slfbp 263 1 The the DT cord-328082-7c7slfbp 263 2 distribution distribution NN cord-328082-7c7slfbp 263 3 and and CC cord-328082-7c7slfbp 263 4 morphology morphology NN cord-328082-7c7slfbp 263 5 of of IN cord-328082-7c7slfbp 263 6 p58positive p58positive JJ cord-328082-7c7slfbp 263 7 elements element NNS cord-328082-7c7slfbp 263 8 in in IN cord-328082-7c7slfbp 263 9 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 263 10 cells cell NNS cord-328082-7c7slfbp 263 11 after after IN cord-328082-7c7slfbp 263 12 incubation incubation NN cord-328082-7c7slfbp 263 13 at at IN cord-328082-7c7slfbp 263 14 20~ 20~ CD cord-328082-7c7slfbp 263 15 in in IN cord-328082-7c7slfbp 263 16 the the DT cord-328082-7c7slfbp 263 17 presence presence NN cord-328082-7c7slfbp 263 18 of of IN cord-328082-7c7slfbp 263 19 10 10 CD cord-328082-7c7slfbp 263 20 mM mm CD cord-328082-7c7slfbp 263 21 caffeine caffeine NN cord-328082-7c7slfbp 263 22 resembled resemble VBD cord-328082-7c7slfbp 263 23 that that IN cord-328082-7c7slfbp 263 24 observed observe VBD cord-328082-7c7slfbp 263 25 earlier early RBR cord-328082-7c7slfbp 263 26 in in IN cord-328082-7c7slfbp 263 27 BFA BFA NNP cord-328082-7c7slfbp 263 28 - - HYPH cord-328082-7c7slfbp 263 29 treated treat VBN cord-328082-7c7slfbp 263 30 cells cell NNS cord-328082-7c7slfbp 263 31 . . . cord-328082-7c7slfbp 264 1 Even even RB cord-328082-7c7slfbp 264 2 though though IN cord-328082-7c7slfbp 264 3 incubation incubation NN cord-328082-7c7slfbp 264 4 at at IN cord-328082-7c7slfbp 264 5 20~ 20~ CD cord-328082-7c7slfbp 264 6 in in IN cord-328082-7c7slfbp 264 7 the the DT cord-328082-7c7slfbp 264 8 presence presence NN cord-328082-7c7slfbp 264 9 of of IN cord-328082-7c7slfbp 264 10 caffeine caffeine NN cord-328082-7c7slfbp 264 11 did do VBD cord-328082-7c7slfbp 264 12 not not RB cord-328082-7c7slfbp 264 13 result result VB cord-328082-7c7slfbp 264 14 in in IN cord-328082-7c7slfbp 264 15 the the DT cord-328082-7c7slfbp 264 16 movement movement NN cord-328082-7c7slfbp 264 17 of of IN cord-328082-7c7slfbp 264 18 a a DT cord-328082-7c7slfbp 264 19 resident resident JJ cord-328082-7c7slfbp 264 20 Golgi Golgi NNP cord-328082-7c7slfbp 264 21 stack stack NN cord-328082-7c7slfbp 264 22 marker marker NN cord-328082-7c7slfbp 264 23 to to IN cord-328082-7c7slfbp 264 24 the the DT cord-328082-7c7slfbp 264 25 ER ER NNP cord-328082-7c7slfbp 264 26 ( ( -LRB- cord-328082-7c7slfbp 264 27 see see VB cord-328082-7c7slfbp 264 28 Fig Fig NNP cord-328082-7c7slfbp 264 29 . . . cord-328082-7c7slfbp 265 1 5 5 CD cord-328082-7c7slfbp 265 2 b b LS cord-328082-7c7slfbp 265 3 ) ) -RRB- cord-328082-7c7slfbp 265 4 , , , cord-328082-7c7slfbp 265 5 it -PRON- PRP cord-328082-7c7slfbp 265 6 did do VBD cord-328082-7c7slfbp 265 7 cause cause VB cord-328082-7c7slfbp 265 8 profound profound JJ cord-328082-7c7slfbp 265 9 effects effect NNS cord-328082-7c7slfbp 265 10 on on IN cord-328082-7c7slfbp 265 11 the the DT cord-328082-7c7slfbp 265 12 organization organization NN cord-328082-7c7slfbp 265 13 of of IN cord-328082-7c7slfbp 265 14 the the DT cord-328082-7c7slfbp 265 15 Golgi Golgi NNP cord-328082-7c7slfbp 265 16 complex complex NN cord-328082-7c7slfbp 265 17 ( ( -LRB- cord-328082-7c7slfbp 265 18 see see VB cord-328082-7c7slfbp 265 19 Fig Fig NNP cord-328082-7c7slfbp 265 20 . . . cord-328082-7c7slfbp 266 1 7 7 CD cord-328082-7c7slfbp 266 2 a a LS cord-328082-7c7slfbp 266 3 ) ) -RRB- cord-328082-7c7slfbp 266 4 . . . cord-328082-7c7slfbp 267 1 ~COP ~COP NFP cord-328082-7c7slfbp 268 1 , , , cord-328082-7c7slfbp 268 2 a a DT cord-328082-7c7slfbp 268 3 protein protein NN cord-328082-7c7slfbp 268 4 known know VBN cord-328082-7c7slfbp 268 5 to to TO cord-328082-7c7slfbp 268 6 be be VB cord-328082-7c7slfbp 268 7 associated associate VBN cord-328082-7c7slfbp 268 8 with with IN cord-328082-7c7slfbp 268 9 the the DT cord-328082-7c7slfbp 268 10 pre pre JJ cord-328082-7c7slfbp 268 11 - - JJ cord-328082-7c7slfbp 268 12 Golgi Golgi NNP cord-328082-7c7slfbp 268 13 and and CC cord-328082-7c7slfbp 268 14 the the DT cord-328082-7c7slfbp 268 15 Golgi Golgi NNP cord-328082-7c7slfbp 268 16 stack stack NN cord-328082-7c7slfbp 268 17 membranes membrane NNS cord-328082-7c7slfbp 268 18 ( ( -LRB- cord-328082-7c7slfbp 268 19 Duden Duden NNP cord-328082-7c7slfbp 268 20 et et FW cord-328082-7c7slfbp 268 21 al al NNP cord-328082-7c7slfbp 268 22 . . NNP cord-328082-7c7slfbp 268 23 , , , cord-328082-7c7slfbp 268 24 1991 1991 CD cord-328082-7c7slfbp 268 25 ) ) -RRB- cord-328082-7c7slfbp 268 26 is be VBZ cord-328082-7c7slfbp 268 27 released release VBN cord-328082-7c7slfbp 268 28 rapidly rapidly RB cord-328082-7c7slfbp 268 29 from from IN cord-328082-7c7slfbp 268 30 the the DT cord-328082-7c7slfbp 268 31 Golgi Golgi NNP cord-328082-7c7slfbp 268 32 membranes membrane NNS cord-328082-7c7slfbp 268 33 in in IN cord-328082-7c7slfbp 268 34 cells cell NNS cord-328082-7c7slfbp 268 35 treated treat VBN cord-328082-7c7slfbp 268 36 with with IN cord-328082-7c7slfbp 268 37 BFA BFA NNP cord-328082-7c7slfbp 268 38 . . . cord-328082-7c7slfbp 269 1 We -PRON- PRP cord-328082-7c7slfbp 269 2 therefore therefore RB cord-328082-7c7slfbp 269 3 tested test VBD cord-328082-7c7slfbp 269 4 whether whether IN cord-328082-7c7slfbp 269 5 caffeine caffeine NN cord-328082-7c7slfbp 269 6 would would MD cord-328082-7c7slfbp 269 7 have have VB cord-328082-7c7slfbp 269 8 an an DT cord-328082-7c7slfbp 269 9 effect effect NN cord-328082-7c7slfbp 269 10 on on IN cord-328082-7c7slfbp 269 11 the the DT cord-328082-7c7slfbp 269 12 localization localization NN cord-328082-7c7slfbp 269 13 of of IN cord-328082-7c7slfbp 269 14 E e NN cord-328082-7c7slfbp 269 15 - - NN cord-328082-7c7slfbp 269 16 COP cop NN cord-328082-7c7slfbp 269 17 . . . cord-328082-7c7slfbp 270 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 271 1 cells cell NNS cord-328082-7c7slfbp 271 2 were be VBD cord-328082-7c7slfbp 271 3 incubated incubate VBN cord-328082-7c7slfbp 271 4 at at IN cord-328082-7c7slfbp 271 5 20~ 20~ CD cord-328082-7c7slfbp 271 6 in in IN cord-328082-7c7slfbp 271 7 the the DT cord-328082-7c7slfbp 271 8 presence presence NN cord-328082-7c7slfbp 271 9 or or CC cord-328082-7c7slfbp 271 10 absence absence NN cord-328082-7c7slfbp 271 11 of of IN cord-328082-7c7slfbp 271 12 10 10 CD cord-328082-7c7slfbp 271 13 mM mm CD cord-328082-7c7slfbp 271 14 caffeine caffeine NN cord-328082-7c7slfbp 271 15 , , , cord-328082-7c7slfbp 271 16 fixed fix VBN cord-328082-7c7slfbp 271 17 , , , cord-328082-7c7slfbp 271 18 and and CC cord-328082-7c7slfbp 271 19 processed process VBN cord-328082-7c7slfbp 271 20 for for IN cord-328082-7c7slfbp 271 21 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 271 22 microscopy microscopy NN cord-328082-7c7slfbp 271 23 . . . cord-328082-7c7slfbp 272 1 In in IN cord-328082-7c7slfbp 272 2 Fig Fig NNP cord-328082-7c7slfbp 272 3 . . . cord-328082-7c7slfbp 273 1 9 9 CD cord-328082-7c7slfbp 273 2 a a DT cord-328082-7c7slfbp 273 3 , , , cord-328082-7c7slfbp 273 4 the the DT cord-328082-7c7slfbp 273 5 distribution distribution NN cord-328082-7c7slfbp 273 6 of of IN cord-328082-7c7slfbp 273 7 E e NN cord-328082-7c7slfbp 273 8 - - NN cord-328082-7c7slfbp 273 9 COP cop NN cord-328082-7c7slfbp 273 10 is be VBZ cord-328082-7c7slfbp 273 11 shown show VBN cord-328082-7c7slfbp 273 12 in in IN cord-328082-7c7slfbp 273 13 cells cell NNS cord-328082-7c7slfbp 273 14 before before IN cord-328082-7c7slfbp 273 15 any any DT cord-328082-7c7slfbp 273 16 treatments treatment NNS cord-328082-7c7slfbp 273 17 . . . cord-328082-7c7slfbp 274 1 ~COP ~COP NFP cord-328082-7c7slfbp 275 1 has have VBZ cord-328082-7c7slfbp 275 2 a a DT cord-328082-7c7slfbp 275 3 strong strong JJ cord-328082-7c7slfbp 275 4 perinuclear perinuclear NN cord-328082-7c7slfbp 275 5 localization localization NN cord-328082-7c7slfbp 275 6 and and CC cord-328082-7c7slfbp 276 1 also also RB cord-328082-7c7slfbp 276 2 the the DT cord-328082-7c7slfbp 276 3 labeling labeling NN cord-328082-7c7slfbp 276 4 of of IN cord-328082-7c7slfbp 276 5 cytoplasmic cytoplasmic JJ cord-328082-7c7slfbp 276 6 vesicular vesicular JJ cord-328082-7c7slfbp 276 7 elements element NNS cord-328082-7c7slfbp 276 8 is be VBZ cord-328082-7c7slfbp 276 9 observed observe VBN cord-328082-7c7slfbp 276 10 ( ( -LRB- cord-328082-7c7slfbp 276 11 Fig Fig NNP cord-328082-7c7slfbp 276 12 . . . cord-328082-7c7slfbp 276 13 9 9 CD cord-328082-7c7slfbp 276 14 a a LS cord-328082-7c7slfbp 276 15 ) ) -RRB- cord-328082-7c7slfbp 276 16 . . . cord-328082-7c7slfbp 277 1 When when WRB cord-328082-7c7slfbp 277 2 cells cell NNS cord-328082-7c7slfbp 277 3 were be VBD cord-328082-7c7slfbp 277 4 incubated incubate VBN cord-328082-7c7slfbp 277 5 at at IN cord-328082-7c7slfbp 277 6 20~ 20~ CD cord-328082-7c7slfbp 277 7 for for IN cord-328082-7c7slfbp 277 8 60 60 CD cord-328082-7c7slfbp 277 9 min min NN cord-328082-7c7slfbp 277 10 in in IN cord-328082-7c7slfbp 277 11 the the DT cord-328082-7c7slfbp 277 12 absence absence NN cord-328082-7c7slfbp 277 13 of of IN cord-328082-7c7slfbp 277 14 caffeine caffeine NN cord-328082-7c7slfbp 277 15 ( ( -LRB- cord-328082-7c7slfbp 277 16 Fig Fig NNP cord-328082-7c7slfbp 277 17 . . . cord-328082-7c7slfbp 277 18 9 9 CD cord-328082-7c7slfbp 277 19 b b NN cord-328082-7c7slfbp 277 20 ) ) -RRB- cord-328082-7c7slfbp 277 21 , , , cord-328082-7c7slfbp 277 22 the the DT cord-328082-7c7slfbp 277 23 distribution distribution NN cord-328082-7c7slfbp 277 24 of of IN cord-328082-7c7slfbp 277 25 /~-COP /~-COP NNP cord-328082-7c7slfbp 277 26 was be VBD cord-328082-7c7slfbp 277 27 essentially essentially RB cord-328082-7c7slfbp 277 28 the the DT cord-328082-7c7slfbp 277 29 same same JJ cord-328082-7c7slfbp 277 30 as as IN cord-328082-7c7slfbp 277 31 in in IN cord-328082-7c7slfbp 277 32 control control NN cord-328082-7c7slfbp 277 33 cells cell NNS cord-328082-7c7slfbp 277 34 without without IN cord-328082-7c7slfbp 277 35 any any DT cord-328082-7c7slfbp 277 36 treatments treatment NNS cord-328082-7c7slfbp 277 37 ( ( -LRB- cord-328082-7c7slfbp 277 38 Fig Fig NNP cord-328082-7c7slfbp 277 39 . . . cord-328082-7c7slfbp 277 40 9 9 CD cord-328082-7c7slfbp 277 41 a a LS cord-328082-7c7slfbp 277 42 ) ) -RRB- cord-328082-7c7slfbp 277 43 . . . cord-328082-7c7slfbp 278 1 A a DT cord-328082-7c7slfbp 278 2 60-min 60-min CD cord-328082-7c7slfbp 278 3 incubation incubation NN cord-328082-7c7slfbp 278 4 at at IN cord-328082-7c7slfbp 278 5 20~ 20~ CD cord-328082-7c7slfbp 278 6 with with IN cord-328082-7c7slfbp 278 7 10 10 CD cord-328082-7c7slfbp 278 8 mM mm CD cord-328082-7c7slfbp 278 9 caffeine caffeine NN cord-328082-7c7slfbp 278 10 did do VBD cord-328082-7c7slfbp 278 11 not not RB cord-328082-7c7slfbp 278 12 have have VB cord-328082-7c7slfbp 278 13 any any DT cord-328082-7c7slfbp 278 14 detectable detectable JJ cord-328082-7c7slfbp 278 15 effect effect NN cord-328082-7c7slfbp 278 16 on on IN cord-328082-7c7slfbp 278 17 the the DT cord-328082-7c7slfbp 278 18 distribution distribution NN cord-328082-7c7slfbp 278 19 of/~-COP of/~-cop CD cord-328082-7c7slfbp 279 1 ( ( -LRB- cord-328082-7c7slfbp 279 2 Fig Fig NNP cord-328082-7c7slfbp 279 3 . . . cord-328082-7c7slfbp 279 4 9 9 CD cord-328082-7c7slfbp 279 5 c c NN cord-328082-7c7slfbp 279 6 ) ) -RRB- cord-328082-7c7slfbp 279 7 . . . cord-328082-7c7slfbp 280 1 Because because IN cord-328082-7c7slfbp 280 2 of of IN cord-328082-7c7slfbp 280 3 the the DT cord-328082-7c7slfbp 280 4 strong strong JJ cord-328082-7c7slfbp 280 5 perinuclear perinuclear NNP cord-328082-7c7slfbp 280 6 labeling labeling NN cord-328082-7c7slfbp 280 7 of of IN cord-328082-7c7slfbp 280 8 E e NN cord-328082-7c7slfbp 280 9 - - NN cord-328082-7c7slfbp 280 10 COP cop NN cord-328082-7c7slfbp 280 11 , , , cord-328082-7c7slfbp 280 12 possible possible JJ cord-328082-7c7slfbp 280 13 changes change NNS cord-328082-7c7slfbp 280 14 in in IN cord-328082-7c7slfbp 280 15 the the DT cord-328082-7c7slfbp 280 16 distribution distribution NN cord-328082-7c7slfbp 280 17 of of IN cord-328082-7c7slfbp 280 18 pre pre JJ cord-328082-7c7slfbp 280 19 - - JJ cord-328082-7c7slfbp 280 20 and and CC cord-328082-7c7slfbp 280 21 cis cis NNP cord-328082-7c7slfbp 280 22 - - HYPH cord-328082-7c7slfbp 280 23 Golgi Golgi NNP cord-328082-7c7slfbp 280 24 elements element NNS cord-328082-7c7slfbp 280 25 were be VBD cord-328082-7c7slfbp 280 26 undetectable undetectable JJ cord-328082-7c7slfbp 280 27 in in IN cord-328082-7c7slfbp 280 28 the the DT cord-328082-7c7slfbp 280 29 experiment experiment NN cord-328082-7c7slfbp 280 30 . . . cord-328082-7c7slfbp 281 1 This this DT cord-328082-7c7slfbp 281 2 result result NN cord-328082-7c7slfbp 281 3 nevertheless nevertheless RB cord-328082-7c7slfbp 281 4 suggests suggest VBZ cord-328082-7c7slfbp 281 5 that that IN cord-328082-7c7slfbp 281 6 BFA BFA NNP cord-328082-7c7slfbp 281 7 and and CC cord-328082-7c7slfbp 281 8 caffeine caffeine NN cord-328082-7c7slfbp 281 9 affect affect VBP cord-328082-7c7slfbp 281 10 the the DT cord-328082-7c7slfbp 281 11 membranes membrane NNS cord-328082-7c7slfbp 281 12 of of IN cord-328082-7c7slfbp 281 13 the the DT cord-328082-7c7slfbp 281 14 Golgi Golgi NNP cord-328082-7c7slfbp 281 15 complex complex NN cord-328082-7c7slfbp 281 16 through through IN cord-328082-7c7slfbp 281 17 different different JJ cord-328082-7c7slfbp 281 18 mechanisms mechanism NNS cord-328082-7c7slfbp 281 19 . . . cord-328082-7c7slfbp 282 1 Recent recent JJ cord-328082-7c7slfbp 282 2 work work NN cord-328082-7c7slfbp 282 3 by by IN cord-328082-7c7slfbp 282 4 many many JJ cord-328082-7c7slfbp 282 5 laboratories laboratory NNS cord-328082-7c7slfbp 282 6 has have VBZ cord-328082-7c7slfbp 282 7 shown show VBN cord-328082-7c7slfbp 282 8 that that IN cord-328082-7c7slfbp 282 9 the the DT cord-328082-7c7slfbp 282 10 transport transport NN cord-328082-7c7slfbp 282 11 between between IN cord-328082-7c7slfbp 282 12 the the DT cord-328082-7c7slfbp 282 13 ER ER NNP cord-328082-7c7slfbp 282 14 and and CC cord-328082-7c7slfbp 282 15 the the DT cord-328082-7c7slfbp 282 16 Golgi Golgi NNP cord-328082-7c7slfbp 282 17 is be VBZ cord-328082-7c7slfbp 282 18 mediated mediate VBN cord-328082-7c7slfbp 282 19 by by IN cord-328082-7c7slfbp 282 20 transport transport NN cord-328082-7c7slfbp 282 21 vesicles vesicle NNS cord-328082-7c7slfbp 282 22 . . . cord-328082-7c7slfbp 283 1 These these DT cord-328082-7c7slfbp 283 2 transport transport NN cord-328082-7c7slfbp 283 3 vesicles vesicle NNS cord-328082-7c7slfbp 283 4 appear appear VBP cord-328082-7c7slfbp 283 5 to to TO cord-328082-7c7slfbp 283 6 exit exit VB cord-328082-7c7slfbp 283 7 throughout throughout IN cord-328082-7c7slfbp 283 8 the the DT cord-328082-7c7slfbp 283 9 ER er NN cord-328082-7c7slfbp 283 10 and and CC cord-328082-7c7slfbp 283 11 to to TO cord-328082-7c7slfbp 283 12 concentrate concentrate VB cord-328082-7c7slfbp 283 13 perinuclearly perinuclearly RB cord-328082-7c7slfbp 283 14 along along IN cord-328082-7c7slfbp 283 15 the the DT cord-328082-7c7slfbp 283 16 microtubules microtubule NNS cord-328082-7c7slfbp 283 17 in in IN cord-328082-7c7slfbp 283 18 vivo vivo NN cord-328082-7c7slfbp 283 19 ( ( -LRB- cord-328082-7c7slfbp 283 20 Saraste Saraste NNP cord-328082-7c7slfbp 283 21 and and CC cord-328082-7c7slfbp 283 22 Svensson Svensson NNP cord-328082-7c7slfbp 283 23 , , , cord-328082-7c7slfbp 283 24 1991 1991 CD cord-328082-7c7slfbp 283 25 ) ) -RRB- cord-328082-7c7slfbp 283 26 . . . cord-328082-7c7slfbp 284 1 Only only RB cord-328082-7c7slfbp 284 2 a a DT cord-328082-7c7slfbp 284 3 few few JJ cord-328082-7c7slfbp 284 4 markers marker NNS cord-328082-7c7slfbp 284 5 for for IN cord-328082-7c7slfbp 284 6 these these DT cord-328082-7c7slfbp 284 7 intermediate intermediate JJ cord-328082-7c7slfbp 284 8 elements element NNS cord-328082-7c7slfbp 284 9 are be VBP cord-328082-7c7slfbp 284 10 known know VBN cord-328082-7c7slfbp 284 11 . . . cord-328082-7c7slfbp 285 1 Two two CD cord-328082-7c7slfbp 285 2 membrane membrane NN cord-328082-7c7slfbp 285 3 proteins protein NNS cord-328082-7c7slfbp 285 4 , , , cord-328082-7c7slfbp 285 5 p58 p58 NNP cord-328082-7c7slfbp 285 6 ( ( -LRB- cord-328082-7c7slfbp 285 7 Saraste Saraste NNP cord-328082-7c7slfbp 285 8 et et NNP cord-328082-7c7slfbp 285 9 al al NNP cord-328082-7c7slfbp 285 10 . . NNP cord-328082-7c7slfbp 285 11 , , , cord-328082-7c7slfbp 285 12 1987 1987 CD cord-328082-7c7slfbp 285 13 ) ) -RRB- cord-328082-7c7slfbp 285 14 and and CC cord-328082-7c7slfbp 285 15 p53 p53 NN cord-328082-7c7slfbp 285 16 ( ( -LRB- cord-328082-7c7slfbp 285 17 Schweizer Schweizer NNP cord-328082-7c7slfbp 285 18 et et NNP cord-328082-7c7slfbp 285 19 al al NNP cord-328082-7c7slfbp 285 20 . . NNP cord-328082-7c7slfbp 285 21 , , , cord-328082-7c7slfbp 285 22 1988 1988 CD cord-328082-7c7slfbp 285 23 ) ) -RRB- cord-328082-7c7slfbp 285 24 , , , cord-328082-7c7slfbp 285 25 are be VBP cord-328082-7c7slfbp 285 26 localized localize VBN cord-328082-7c7slfbp 285 27 to to IN cord-328082-7c7slfbp 285 28 the the DT cord-328082-7c7slfbp 285 29 intermediate intermediate JJ cord-328082-7c7slfbp 285 30 elements element NNS cord-328082-7c7slfbp 285 31 and and CC cord-328082-7c7slfbp 285 32 the the DT cord-328082-7c7slfbp 285 33 cis cis NNP cord-328082-7c7slfbp 285 34 - - HYPH cord-328082-7c7slfbp 285 35 Golgi Golgi NNP cord-328082-7c7slfbp 285 36 . . . cord-328082-7c7slfbp 286 1 In in IN cord-328082-7c7slfbp 286 2 addition addition NN cord-328082-7c7slfbp 286 3 , , , cord-328082-7c7slfbp 286 4 the the DT cord-328082-7c7slfbp 286 5 KDEL KDEL NNP cord-328082-7c7slfbp 286 6 receptor receptor NN cord-328082-7c7slfbp 286 7 , , , cord-328082-7c7slfbp 286 8 responsible responsible JJ cord-328082-7c7slfbp 286 9 for for IN cord-328082-7c7slfbp 286 10 the the DT cord-328082-7c7slfbp 286 11 retention retention NN cord-328082-7c7slfbp 286 12 of of IN cord-328082-7c7slfbp 286 13 soluble soluble JJ cord-328082-7c7slfbp 286 14 ER ER NNP cord-328082-7c7slfbp 286 15 proteins protein NNS cord-328082-7c7slfbp 286 16 ( ( -LRB- cord-328082-7c7slfbp 286 17 Pelham Pelham NNP cord-328082-7c7slfbp 286 18 , , , cord-328082-7c7slfbp 286 19 1988 1988 CD cord-328082-7c7slfbp 286 20 ; ; : cord-328082-7c7slfbp 286 21 Pelham Pelham NNP cord-328082-7c7slfbp 286 22 , , , cord-328082-7c7slfbp 286 23 1989 1989 CD cord-328082-7c7slfbp 286 24 ) ) -RRB- cord-328082-7c7slfbp 286 25 , , , cord-328082-7c7slfbp 286 26 is be VBZ cord-328082-7c7slfbp 286 27 likely likely JJ cord-328082-7c7slfbp 286 28 to to TO cord-328082-7c7slfbp 286 29 be be VB cord-328082-7c7slfbp 286 30 located locate VBN cord-328082-7c7slfbp 286 31 at at IN cord-328082-7c7slfbp 286 32 least least JJS cord-328082-7c7slfbp 286 33 in in IN cord-328082-7c7slfbp 286 34 some some DT cord-328082-7c7slfbp 286 35 degree degree NN cord-328082-7c7slfbp 286 36 to to IN cord-328082-7c7slfbp 286 37 these these DT cord-328082-7c7slfbp 286 38 membranes membrane NNS cord-328082-7c7slfbp 286 39 . . . cord-328082-7c7slfbp 287 1 There there EX cord-328082-7c7slfbp 287 2 is be VBZ cord-328082-7c7slfbp 287 3 accumulating accumulate VBG cord-328082-7c7slfbp 287 4 knowledge knowledge NN cord-328082-7c7slfbp 287 5 on on IN cord-328082-7c7slfbp 287 6 the the DT cord-328082-7c7slfbp 287 7 com- com- NN cord-328082-7c7slfbp 287 8 in in IN cord-328082-7c7slfbp 287 9 the the DT cord-328082-7c7slfbp 287 10 Golgi Golgi NNP cord-328082-7c7slfbp 287 11 area area NN cord-328082-7c7slfbp 287 12 in in IN cord-328082-7c7slfbp 287 13 cells cell NNS cord-328082-7c7slfbp 287 14 treated treat VBN cord-328082-7c7slfbp 287 15 with with IN cord-328082-7c7slfbp 287 16 caffeine caffeine NN cord-328082-7c7slfbp 287 17 at at IN cord-328082-7c7slfbp 287 18 20~ 20~ CD cord-328082-7c7slfbp 287 19 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 287 20 ceils ceil NNS cord-328082-7c7slfbp 287 21 were be VBD cord-328082-7c7slfbp 287 22 incubated incubate VBN cord-328082-7c7slfbp 287 23 at at IN cord-328082-7c7slfbp 287 24 20~ 20~ CD cord-328082-7c7slfbp 287 25 with with IN cord-328082-7c7slfbp 287 26 ( ( -LRB- cord-328082-7c7slfbp 287 27 a a LS cord-328082-7c7slfbp 287 28 ) ) -RRB- cord-328082-7c7slfbp 287 29 or or CC cord-328082-7c7slfbp 287 30 without without IN cord-328082-7c7slfbp 287 31 ( ( -LRB- cord-328082-7c7slfbp 287 32 b b NN cord-328082-7c7slfbp 287 33 ) ) -RRB- cord-328082-7c7slfbp 287 34 10 10 CD cord-328082-7c7slfbp 287 35 mM mm CD cord-328082-7c7slfbp 287 36 caffeine caffeine NN cord-328082-7c7slfbp 287 37 , , , cord-328082-7c7slfbp 287 38 fixed fix VBN cord-328082-7c7slfbp 287 39 , , , cord-328082-7c7slfbp 287 40 and and CC cord-328082-7c7slfbp 287 41 processed process VBN cord-328082-7c7slfbp 287 42 for for IN cord-328082-7c7slfbp 287 43 immunoperoxidase immunoperoxidase NN cord-328082-7c7slfbp 287 44 electron electron NN cord-328082-7c7slfbp 287 45 microscopy microscopy NN cord-328082-7c7slfbp 287 46 with with IN cord-328082-7c7slfbp 287 47 antibodies antibody NNS cord-328082-7c7slfbp 287 48 to to IN cord-328082-7c7slfbp 287 49 p5 p5 NNP cord-328082-7c7slfbp 287 50 & & CC cord-328082-7c7slfbp 287 51 The the DT cord-328082-7c7slfbp 287 52 alteration alteration NN cord-328082-7c7slfbp 287 53 of of IN cord-328082-7c7slfbp 287 54 the the DT cord-328082-7c7slfbp 287 55 Golgi Golgi NNP cord-328082-7c7slfbp 287 56 morphology morphology NN cord-328082-7c7slfbp 287 57 in in IN cord-328082-7c7slfbp 287 58 caffeine caffeine NN cord-328082-7c7slfbp 287 59 - - HYPH cord-328082-7c7slfbp 287 60 treated treat VBN cord-328082-7c7slfbp 287 61 cells cell NNS cord-328082-7c7slfbp 287 62 ( ( -LRB- cord-328082-7c7slfbp 287 63 a a LS cord-328082-7c7slfbp 287 64 ) ) -RRB- cord-328082-7c7slfbp 287 65 can can MD cord-328082-7c7slfbp 287 66 be be VB cord-328082-7c7slfbp 287 67 observed observe VBN cord-328082-7c7slfbp 287 68 . . . cord-328082-7c7slfbp 288 1 Arrows arrow NNS cord-328082-7c7slfbp 288 2 mark mark VBP cord-328082-7c7slfbp 288 3 the the DT cord-328082-7c7slfbp 288 4 p58-positive p58-positive JJ cord-328082-7c7slfbp 288 5 structures structure NNS cord-328082-7c7slfbp 288 6 found find VBN cord-328082-7c7slfbp 288 7 in in IN cord-328082-7c7slfbp 288 8 the the DT cord-328082-7c7slfbp 288 9 Golgi Golgi NNP cord-328082-7c7slfbp 288 10 region region NN cord-328082-7c7slfbp 288 11 both both CC cord-328082-7c7slfbp 288 12 in in IN cord-328082-7c7slfbp 288 13 caffeine caffeine NN cord-328082-7c7slfbp 288 14 - - HYPH cord-328082-7c7slfbp 288 15 treated treat VBN cord-328082-7c7slfbp 288 16 ( ( -LRB- cord-328082-7c7slfbp 288 17 a a NN cord-328082-7c7slfbp 288 18 ) ) -RRB- cord-328082-7c7slfbp 288 19 and and CC cord-328082-7c7slfbp 288 20 in in IN cord-328082-7c7slfbp 288 21 control control NN cord-328082-7c7slfbp 288 22 cells cell NNS cord-328082-7c7slfbp 288 23 ( ( -LRB- cord-328082-7c7slfbp 288 24 b b NN cord-328082-7c7slfbp 288 25 ) ) -RRB- cord-328082-7c7slfbp 288 26 . . . cord-328082-7c7slfbp 289 1 In in IN cord-328082-7c7slfbp 289 2 caffeine caffeine NN cord-328082-7c7slfbp 289 3 - - HYPH cord-328082-7c7slfbp 289 4 treated treat VBN cord-328082-7c7slfbp 289 5 cells cell NNS cord-328082-7c7slfbp 289 6 ( ( -LRB- cord-328082-7c7slfbp 289 7 a a LS cord-328082-7c7slfbp 289 8 ) ) -RRB- cord-328082-7c7slfbp 289 9 the the DT cord-328082-7c7slfbp 289 10 amount amount NN cord-328082-7c7slfbp 289 11 of of IN cord-328082-7c7slfbp 289 12 p58 p58 NN cord-328082-7c7slfbp 289 13 - - HYPH cord-328082-7c7slfbp 289 14 1abeled 1abeled CD cord-328082-7c7slfbp 289 15 membranes membrane NNS cord-328082-7c7slfbp 289 16 seems seem VBZ cord-328082-7c7slfbp 289 17 to to TO cord-328082-7c7slfbp 289 18 be be VB cord-328082-7c7slfbp 289 19 lower low JJR cord-328082-7c7slfbp 289 20 compared compare VBN cord-328082-7c7slfbp 289 21 with with IN cord-328082-7c7slfbp 289 22 that that DT cord-328082-7c7slfbp 289 23 observed observe VBN cord-328082-7c7slfbp 289 24 in in IN cord-328082-7c7slfbp 289 25 control control NN cord-328082-7c7slfbp 289 26 cells cell NNS cord-328082-7c7slfbp 289 27 ( ( -LRB- cord-328082-7c7slfbp 289 28 b b NN cord-328082-7c7slfbp 289 29 ) ) -RRB- cord-328082-7c7slfbp 289 30 . . . cord-328082-7c7slfbp 290 1 Bar bar NN cord-328082-7c7slfbp 290 2 , , , cord-328082-7c7slfbp 290 3 500 500 CD cord-328082-7c7slfbp 290 4 nm nm NN cord-328082-7c7slfbp 290 5 . . . cord-328082-7c7slfbp 291 1 Figure figure NN cord-328082-7c7slfbp 291 2 9 9 CD cord-328082-7c7slfbp 291 3 . . . cord-328082-7c7slfbp 292 1 Effect effect NN cord-328082-7c7slfbp 292 2 of of IN cord-328082-7c7slfbp 292 3 caffeine caffeine NN cord-328082-7c7slfbp 292 4 at at IN cord-328082-7c7slfbp 292 5 20~ 20~ CD cord-328082-7c7slfbp 292 6 on on IN cord-328082-7c7slfbp 292 7 the the DT cord-328082-7c7slfbp 292 8 localization localization NN cord-328082-7c7slfbp 292 9 of/3-COP of/3-COP NNP cord-328082-7c7slfbp 292 10 in in IN cord-328082-7c7slfbp 292 11 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 292 12 cells cell NNS cord-328082-7c7slfbp 292 13 . . . cord-328082-7c7slfbp 293 1 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 294 1 cells cell NNS cord-328082-7c7slfbp 294 2 were be VBD cord-328082-7c7slfbp 294 3 grown grow VBN cord-328082-7c7slfbp 294 4 for for IN cord-328082-7c7slfbp 294 5 60 60 CD cord-328082-7c7slfbp 294 6 min min NN cord-328082-7c7slfbp 294 7 in in IN cord-328082-7c7slfbp 294 8 the the DT cord-328082-7c7slfbp 294 9 presence presence NN cord-328082-7c7slfbp 294 10 ( ( -LRB- cord-328082-7c7slfbp 294 11 c c NN cord-328082-7c7slfbp 294 12 ) ) -RRB- cord-328082-7c7slfbp 294 13 or or CC cord-328082-7c7slfbp 294 14 absence absence NN cord-328082-7c7slfbp 294 15 ( ( -LRB- cord-328082-7c7slfbp 294 16 b b NN cord-328082-7c7slfbp 294 17 ) ) -RRB- cord-328082-7c7slfbp 294 18 of of IN cord-328082-7c7slfbp 294 19 10 10 CD cord-328082-7c7slfbp 294 20 mM mm CD cord-328082-7c7slfbp 294 21 caffeine caffeine NN cord-328082-7c7slfbp 294 22 at at IN cord-328082-7c7slfbp 294 23 20~ 20~ CD cord-328082-7c7slfbp 294 24 fixed fix VBN cord-328082-7c7slfbp 294 25 , , , cord-328082-7c7slfbp 294 26 and and CC cord-328082-7c7slfbp 294 27 processed process VBN cord-328082-7c7slfbp 294 28 for for IN cord-328082-7c7slfbp 294 29 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 294 30 with with IN cord-328082-7c7slfbp 294 31 antibodies antibody NNS cord-328082-7c7slfbp 294 32 to to IN cord-328082-7c7slfbp 294 33 fl fl NNP cord-328082-7c7slfbp 294 34 - - HYPH cord-328082-7c7slfbp 294 35 COP COP NNP cord-328082-7c7slfbp 294 36 , , , cord-328082-7c7slfbp 294 37 a a DT cord-328082-7c7slfbp 294 38 shows show VBZ cord-328082-7c7slfbp 294 39 the the DT cord-328082-7c7slfbp 294 40 normal normal JJ cord-328082-7c7slfbp 294 41 distribution distribution NN cord-328082-7c7slfbp 294 42 of of IN cord-328082-7c7slfbp 294 43 fl fl NNP cord-328082-7c7slfbp 294 44 - - HYPH cord-328082-7c7slfbp 294 45 COP cop NN cord-328082-7c7slfbp 294 46 in in IN cord-328082-7c7slfbp 294 47 BHK-21 BHK-21 NNP cord-328082-7c7slfbp 294 48 cells cell NNS cord-328082-7c7slfbp 294 49 . . . cord-328082-7c7slfbp 295 1 Caffeine caffeine NN cord-328082-7c7slfbp 295 2 at at IN cord-328082-7c7slfbp 295 3 reduced reduce VBN cord-328082-7c7slfbp 295 4 temperature temperature NN cord-328082-7c7slfbp 295 5 does do VBZ cord-328082-7c7slfbp 295 6 not not RB cord-328082-7c7slfbp 295 7 seem seem VB cord-328082-7c7slfbp 295 8 to to TO cord-328082-7c7slfbp 295 9 affect affect VB cord-328082-7c7slfbp 295 10 the the DT cord-328082-7c7slfbp 295 11 distribution distribution NN cord-328082-7c7slfbp 295 12 of of IN cord-328082-7c7slfbp 295 13 fl fl NNP cord-328082-7c7slfbp 295 14 - - HYPH cord-328082-7c7slfbp 295 15 COP COP NNP cord-328082-7c7slfbp 295 16 ( ( -LRB- cord-328082-7c7slfbp 295 17 c c NN cord-328082-7c7slfbp 295 18 ) ) -RRB- cord-328082-7c7slfbp 295 19 compared compare VBN cord-328082-7c7slfbp 295 20 with with IN cord-328082-7c7slfbp 295 21 that that DT cord-328082-7c7slfbp 295 22 in in IN cord-328082-7c7slfbp 295 23 control control NN cord-328082-7c7slfbp 295 24 cells cell NNS cord-328082-7c7slfbp 295 25 incubated incubate VBN cord-328082-7c7slfbp 295 26 only only RB cord-328082-7c7slfbp 295 27 at at IN cord-328082-7c7slfbp 295 28 20~ 20~ CD cord-328082-7c7slfbp 295 29 ( ( -LRB- cord-328082-7c7slfbp 295 30 b b NN cord-328082-7c7slfbp 295 31 ) ) -RRB- cord-328082-7c7slfbp 295 32 or or CC cord-328082-7c7slfbp 295 33 to to IN cord-328082-7c7slfbp 295 34 that that DT cord-328082-7c7slfbp 295 35 observed observe VBN cord-328082-7c7slfbp 295 36 in in IN cord-328082-7c7slfbp 295 37 cells cell NNS cord-328082-7c7slfbp 295 38 without without IN cord-328082-7c7slfbp 295 39 any any DT cord-328082-7c7slfbp 295 40 treatment treatment NN cord-328082-7c7slfbp 295 41 ( ( -LRB- cord-328082-7c7slfbp 295 42 a a NN cord-328082-7c7slfbp 295 43 ) ) -RRB- cord-328082-7c7slfbp 295 44 . . . cord-328082-7c7slfbp 296 1 Bar bar NN cord-328082-7c7slfbp 296 2 , , , cord-328082-7c7slfbp 296 3 20 20 CD cord-328082-7c7slfbp 296 4 # # NN cord-328082-7c7slfbp 296 5 m. m. NN cord-328082-7c7slfbp 296 6 plexity plexity NN cord-328082-7c7slfbp 296 7 of of IN cord-328082-7c7slfbp 296 8 the the DT cord-328082-7c7slfbp 296 9 molecular molecular JJ cord-328082-7c7slfbp 296 10 machinery machinery NN cord-328082-7c7slfbp 296 11 involved involve VBN cord-328082-7c7slfbp 296 12 in in IN cord-328082-7c7slfbp 296 13 the the DT cord-328082-7c7slfbp 296 14 ER er NN cord-328082-7c7slfbp 296 15 - - HYPH cord-328082-7c7slfbp 296 16 to to IN cord-328082-7c7slfbp 296 17 - - HYPH cord-328082-7c7slfbp 296 18 Golgi golgi NN cord-328082-7c7slfbp 296 19 transport transport NN cord-328082-7c7slfbp 296 20 ( ( -LRB- cord-328082-7c7slfbp 296 21 for for IN cord-328082-7c7slfbp 296 22 review review NN cord-328082-7c7slfbp 296 23 see see VBP cord-328082-7c7slfbp 296 24 Rothman Rothman NNP cord-328082-7c7slfbp 296 25 and and CC cord-328082-7c7slfbp 296 26 Orci Orci NNP cord-328082-7c7slfbp 296 27 , , , cord-328082-7c7slfbp 296 28 1992 1992 CD cord-328082-7c7slfbp 296 29 ) ) -RRB- cord-328082-7c7slfbp 296 30 , , , cord-328082-7c7slfbp 296 31 based base VBN cord-328082-7c7slfbp 296 32 on on IN cord-328082-7c7slfbp 296 33 work work NN cord-328082-7c7slfbp 296 34 with with IN cord-328082-7c7slfbp 296 35 yeast yeast NN cord-328082-7c7slfbp 296 36 mutants mutant NNS cord-328082-7c7slfbp 296 37 ( ( -LRB- cord-328082-7c7slfbp 297 1 Novick Novick NNP cord-328082-7c7slfbp 297 2 et et NNP cord-328082-7c7slfbp 297 3 al al NNP cord-328082-7c7slfbp 297 4 . . NNP cord-328082-7c7slfbp 297 5 , , , cord-328082-7c7slfbp 297 6 1980 1980 CD cord-328082-7c7slfbp 297 7 ) ) -RRB- cord-328082-7c7slfbp 297 8 and and CC cord-328082-7c7slfbp 297 9 cell cell NN cord-328082-7c7slfbp 297 10 - - HYPH cord-328082-7c7slfbp 297 11 free free JJ cord-328082-7c7slfbp 297 12 transport transport NN cord-328082-7c7slfbp 297 13 assays assay NNS cord-328082-7c7slfbp 297 14 ( ( -LRB- cord-328082-7c7slfbp 297 15 Fries Fries NNP cord-328082-7c7slfbp 297 16 and and CC cord-328082-7c7slfbp 297 17 Rothman Rothman NNP cord-328082-7c7slfbp 297 18 , , , cord-328082-7c7slfbp 297 19 1980 1980 CD cord-328082-7c7slfbp 297 20 ; ; : cord-328082-7c7slfbp 297 21 Balch Balch NNP cord-328082-7c7slfbp 297 22 et et NNP cord-328082-7c7slfbp 297 23 al al NNP cord-328082-7c7slfbp 297 24 . . NNP cord-328082-7c7slfbp 297 25 , , , cord-328082-7c7slfbp 297 26 1984 1984 CD cord-328082-7c7slfbp 297 27 ) ) -RRB- cord-328082-7c7slfbp 297 28 . . . cord-328082-7c7slfbp 298 1 In in IN cord-328082-7c7slfbp 298 2 studies study NNS cord-328082-7c7slfbp 298 3 concerning concern VBG cord-328082-7c7slfbp 298 4 the the DT cord-328082-7c7slfbp 298 5 organization organization NN cord-328082-7c7slfbp 298 6 of of IN cord-328082-7c7slfbp 298 7 the the DT cord-328082-7c7slfbp 298 8 anterograde anterograde JJ cord-328082-7c7slfbp 298 9 and and CC cord-328082-7c7slfbp 298 10 the the DT cord-328082-7c7slfbp 298 11 retrograde retrograde JJ cord-328082-7c7slfbp 298 12 membrane membrane NN cord-328082-7c7slfbp 298 13 traffic traffic NN cord-328082-7c7slfbp 298 14 , , , cord-328082-7c7slfbp 298 15 the the DT cord-328082-7c7slfbp 298 16 reversible reversible JJ cord-328082-7c7slfbp 298 17 effect effect NN cord-328082-7c7slfbp 298 18 of of IN cord-328082-7c7slfbp 298 19 BFA BFA NNP cord-328082-7c7slfbp 298 20 on on IN cord-328082-7c7slfbp 298 21 the the DT cord-328082-7c7slfbp 298 22 organization organization NN cord-328082-7c7slfbp 298 23 of of IN cord-328082-7c7slfbp 298 24 the the DT cord-328082-7c7slfbp 298 25 Golgi Golgi NNP cord-328082-7c7slfbp 298 26 complex complex NN cord-328082-7c7slfbp 298 27 has have VBZ cord-328082-7c7slfbp 298 28 provided provide VBN cord-328082-7c7slfbp 298 29 important important JJ cord-328082-7c7slfbp 298 30 new new JJ cord-328082-7c7slfbp 298 31 information information NN cord-328082-7c7slfbp 298 32 ( ( -LRB- cord-328082-7c7slfbp 298 33 Pelham Pelham NNP cord-328082-7c7slfbp 298 34 , , , cord-328082-7c7slfbp 298 35 1991 1991 CD cord-328082-7c7slfbp 298 36 ; ; : cord-328082-7c7slfbp 298 37 Klausner Klausner NNP cord-328082-7c7slfbp 298 38 et et NNP cord-328082-7c7slfbp 298 39 al al NNP cord-328082-7c7slfbp 298 40 . . NNP cord-328082-7c7slfbp 298 41 , , , cord-328082-7c7slfbp 298 42 1992 1992 CD cord-328082-7c7slfbp 298 43 ) ) -RRB- cord-328082-7c7slfbp 298 44 . . . cord-328082-7c7slfbp 299 1 Our -PRON- PRP$ cord-328082-7c7slfbp 299 2 previous previous JJ cord-328082-7c7slfbp 299 3 report report NN cord-328082-7c7slfbp 299 4 and and CC cord-328082-7c7slfbp 299 5 the the DT cord-328082-7c7slfbp 299 6 results result NNS cord-328082-7c7slfbp 299 7 in in IN cord-328082-7c7slfbp 299 8 the the DT cord-328082-7c7slfbp 299 9 present present JJ cord-328082-7c7slfbp 299 10 study study NN cord-328082-7c7slfbp 299 11 suggest suggest VBP cord-328082-7c7slfbp 299 12 that that IN cord-328082-7c7slfbp 299 13 caffeine caffeine NN cord-328082-7c7slfbp 299 14 is be VBZ cord-328082-7c7slfbp 299 15 a a DT cord-328082-7c7slfbp 299 16 new new JJ cord-328082-7c7slfbp 299 17 potential potential JJ cord-328082-7c7slfbp 299 18 tool tool NN cord-328082-7c7slfbp 299 19 to to TO cord-328082-7c7slfbp 299 20 dissect dissect VB cord-328082-7c7slfbp 299 21 steps step NNS cord-328082-7c7slfbp 299 22 in in IN cord-328082-7c7slfbp 299 23 the the DT cord-328082-7c7slfbp 299 24 exocytic exocytic JJ cord-328082-7c7slfbp 299 25 pathway pathway NN cord-328082-7c7slfbp 299 26 . . . cord-328082-7c7slfbp 300 1 Caffeine caffeine NN cord-328082-7c7slfbp 300 2 is be VBZ cord-328082-7c7slfbp 300 3 known know VBN cord-328082-7c7slfbp 300 4 to to TO cord-328082-7c7slfbp 300 5 inhibit inhibit VB cord-328082-7c7slfbp 300 6 the the DT cord-328082-7c7slfbp 300 7 enzyme enzyme NN cord-328082-7c7slfbp 300 8 phosphodiesterase phosphodiesterase NN cord-328082-7c7slfbp 300 9 ( ( -LRB- cord-328082-7c7slfbp 300 10 Butcher Butcher NNP cord-328082-7c7slfbp 300 11 and and CC cord-328082-7c7slfbp 300 12 Sutherland Sutherland NNP cord-328082-7c7slfbp 300 13 , , , cord-328082-7c7slfbp 300 14 1962 1962 CD cord-328082-7c7slfbp 300 15 ) ) -RRB- cord-328082-7c7slfbp 300 16 , , , cord-328082-7c7slfbp 300 17 thus thus RB cord-328082-7c7slfbp 300 18 resulting result VBG cord-328082-7c7slfbp 300 19 in in IN cord-328082-7c7slfbp 300 20 elevated elevated JJ cord-328082-7c7slfbp 300 21 levels level NNS cord-328082-7c7slfbp 300 22 of of IN cord-328082-7c7slfbp 300 23 cAMP camp NN cord-328082-7c7slfbp 300 24 inside inside IN cord-328082-7c7slfbp 300 25 the the DT cord-328082-7c7slfbp 300 26 cells cell NNS cord-328082-7c7slfbp 300 27 . . . cord-328082-7c7slfbp 301 1 Forskolin forskolin NN cord-328082-7c7slfbp 301 2 , , , cord-328082-7c7slfbp 301 3 which which WDT cord-328082-7c7slfbp 301 4 is be VBZ cord-328082-7c7slfbp 301 5 known know VBN cord-328082-7c7slfbp 301 6 to to TO cord-328082-7c7slfbp 301 7 elevate elevate VB cord-328082-7c7slfbp 301 8 cAMP camp NN cord-328082-7c7slfbp 301 9 levels level NNS cord-328082-7c7slfbp 301 10 inside inside IN cord-328082-7c7slfbp 301 11 cells cell NNS cord-328082-7c7slfbp 301 12 , , , cord-328082-7c7slfbp 301 13 did do VBD cord-328082-7c7slfbp 301 14 not not RB cord-328082-7c7slfbp 301 15 affect affect VB cord-328082-7c7slfbp 301 16 the the DT cord-328082-7c7slfbp 301 17 movement movement NN cord-328082-7c7slfbp 301 18 of of IN cord-328082-7c7slfbp 301 19 SFV SFV NNP cord-328082-7c7slfbp 301 20 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 301 21 ( ( -LRB- cord-328082-7c7slfbp 301 22 data datum NNS cord-328082-7c7slfbp 301 23 not not RB cord-328082-7c7slfbp 301 24 shown show VBN cord-328082-7c7slfbp 301 25 ) ) -RRB- cord-328082-7c7slfbp 301 26 . . . cord-328082-7c7slfbp 302 1 More more RBR cord-328082-7c7slfbp 302 2 recently recently RB cord-328082-7c7slfbp 302 3 caffeine caffeine NN cord-328082-7c7slfbp 302 4 has have VBZ cord-328082-7c7slfbp 302 5 been be VBN cord-328082-7c7slfbp 302 6 shown show VBN cord-328082-7c7slfbp 302 7 to to TO cord-328082-7c7slfbp 302 8 release release VB cord-328082-7c7slfbp 303 1 Ca ca RB cord-328082-7c7slfbp 303 2 2 2 CD cord-328082-7c7slfbp 303 3 § § , cord-328082-7c7slfbp 303 4 ions ion NNS cord-328082-7c7slfbp 303 5 from from IN cord-328082-7c7slfbp 303 6 the the DT cord-328082-7c7slfbp 303 7 intracellular intracellular JJ cord-328082-7c7slfbp 303 8 stores store NNS cord-328082-7c7slfbp 303 9 other other JJ cord-328082-7c7slfbp 303 10 than than IN cord-328082-7c7slfbp 303 11 the the DT cord-328082-7c7slfbp 303 12 inositol inositol NN cord-328082-7c7slfbp 303 13 triphosphate triphosphate NN cord-328082-7c7slfbp 303 14 - - HYPH cord-328082-7c7slfbp 303 15 sensitive sensitive JJ cord-328082-7c7slfbp 303 16 store store NN cord-328082-7c7slfbp 304 1 ( ( -LRB- cord-328082-7c7slfbp 304 2 Burgoyne Burgoyne NNP cord-328082-7c7slfbp 304 3 et et NNP cord-328082-7c7slfbp 304 4 al al NNP cord-328082-7c7slfbp 304 5 . . NNP cord-328082-7c7slfbp 304 6 , , , cord-328082-7c7slfbp 304 7 1989 1989 CD cord-328082-7c7slfbp 304 8 ) ) -RRB- cord-328082-7c7slfbp 304 9 . . . cord-328082-7c7slfbp 305 1 Also also RB cord-328082-7c7slfbp 305 2 the the DT cord-328082-7c7slfbp 305 3 temperature temperature NN cord-328082-7c7slfbp 305 4 dependence dependence NN cord-328082-7c7slfbp 305 5 of of IN cord-328082-7c7slfbp 305 6 the the DT cord-328082-7c7slfbp 305 7 caffeine caffeine NN cord-328082-7c7slfbp 305 8 - - HYPH cord-328082-7c7slfbp 305 9 induced induce VBN cord-328082-7c7slfbp 305 10 inhibition inhibition NN cord-328082-7c7slfbp 305 11 of of IN cord-328082-7c7slfbp 305 12 membrane membrane NN cord-328082-7c7slfbp 305 13 traffic traffic NN cord-328082-7c7slfbp 305 14 follows follow VBZ cord-328082-7c7slfbp 305 15 interestingly interestingly RB cord-328082-7c7slfbp 305 16 well well RB cord-328082-7c7slfbp 305 17 the the DT cord-328082-7c7slfbp 305 18 conditions condition NNS cord-328082-7c7slfbp 305 19 used use VBN cord-328082-7c7slfbp 305 20 in in IN cord-328082-7c7slfbp 305 21 studies study NNS cord-328082-7c7slfbp 305 22 of of IN cord-328082-7c7slfbp 305 23 the the DT cord-328082-7c7slfbp 305 24 GTP GTP NNP cord-328082-7c7slfbp 305 25 - - HYPH cord-328082-7c7slfbp 305 26 mediated mediate VBN cord-328082-7c7slfbp 305 27 Ca Ca NNP cord-328082-7c7slfbp 305 28 2 2 CD cord-328082-7c7slfbp 305 29 + + CC cord-328082-7c7slfbp 305 30 release release NN cord-328082-7c7slfbp 306 1 ( ( -LRB- cord-328082-7c7slfbp 306 2 Gill Gill NNP cord-328082-7c7slfbp 306 3 et et NNP cord-328082-7c7slfbp 306 4 al al NNP cord-328082-7c7slfbp 306 5 . . NNP cord-328082-7c7slfbp 306 6 , , , cord-328082-7c7slfbp 306 7 1986 1986 CD cord-328082-7c7slfbp 306 8 ) ) -RRB- cord-328082-7c7slfbp 306 9 . . . cord-328082-7c7slfbp 307 1 If if IN cord-328082-7c7slfbp 307 2 the the DT cord-328082-7c7slfbp 307 3 effect effect NN cord-328082-7c7slfbp 307 4 of of IN cord-328082-7c7slfbp 307 5 caffeine caffeine NN cord-328082-7c7slfbp 307 6 on on IN cord-328082-7c7slfbp 307 7 the the DT cord-328082-7c7slfbp 307 8 membrane membrane NN cord-328082-7c7slfbp 307 9 traffic traffic NN cord-328082-7c7slfbp 307 10 and and CC cord-328082-7c7slfbp 307 11 on on IN cord-328082-7c7slfbp 307 12 the the DT cord-328082-7c7slfbp 307 13 calcium calcium NN cord-328082-7c7slfbp 307 14 release release NN cord-328082-7c7slfbp 307 15 are be VBP cord-328082-7c7slfbp 307 16 in in IN cord-328082-7c7slfbp 307 17 fact fact NN cord-328082-7c7slfbp 307 18 related relate VBN cord-328082-7c7slfbp 307 19 , , , cord-328082-7c7slfbp 307 20 this this DT cord-328082-7c7slfbp 307 21 could could MD cord-328082-7c7slfbp 307 22 indicate indicate VB cord-328082-7c7slfbp 307 23 that that IN cord-328082-7c7slfbp 307 24 the the DT cord-328082-7c7slfbp 307 25 ability ability NN cord-328082-7c7slfbp 307 26 of of IN cord-328082-7c7slfbp 307 27 the the DT cord-328082-7c7slfbp 307 28 membranes membrane NNS cord-328082-7c7slfbp 307 29 in in IN cord-328082-7c7slfbp 307 30 the the DT cord-328082-7c7slfbp 307 31 ER er NN cord-328082-7c7slfbp 307 32 - - HYPH cord-328082-7c7slfbp 307 33 to to IN cord-328082-7c7slfbp 307 34 - - HYPH cord-328082-7c7slfbp 307 35 Golgi golgi NN cord-328082-7c7slfbp 307 36 interface interface NN cord-328082-7c7slfbp 307 37 to to TO cord-328082-7c7slfbp 307 38 recycle recycle VB cord-328082-7c7slfbp 307 39 escaped escape VBD cord-328082-7c7slfbp 307 40 , , , cord-328082-7c7slfbp 307 41 resident resident JJ cord-328082-7c7slfbp 307 42 ER ER NNP cord-328082-7c7slfbp 307 43 proteins protein NNS cord-328082-7c7slfbp 307 44 back back RB cord-328082-7c7slfbp 307 45 to to IN cord-328082-7c7slfbp 307 46 the the DT cord-328082-7c7slfbp 307 47 ER ER NNP cord-328082-7c7slfbp 307 48 might may MD cord-328082-7c7slfbp 307 49 be be VB cord-328082-7c7slfbp 307 50 regulated regulate VBN cord-328082-7c7slfbp 307 51 by by IN cord-328082-7c7slfbp 307 52 calcium calcium NN cord-328082-7c7slfbp 307 53 . . . cord-328082-7c7slfbp 308 1 Since since IN cord-328082-7c7slfbp 308 2 , , , cord-328082-7c7slfbp 308 3 on on IN cord-328082-7c7slfbp 308 4 the the DT cord-328082-7c7slfbp 308 5 one one CD cord-328082-7c7slfbp 308 6 hand hand NN cord-328082-7c7slfbp 308 7 , , , cord-328082-7c7slfbp 308 8 caffeine caffeine NN cord-328082-7c7slfbp 308 9 is be VBZ cord-328082-7c7slfbp 308 10 known know VBN cord-328082-7c7slfbp 308 11 to to TO cord-328082-7c7slfbp 308 12 affect affect VB cord-328082-7c7slfbp 308 13 the the DT cord-328082-7c7slfbp 308 14 control control NN cord-328082-7c7slfbp 308 15 mechanisms mechanism NNS cord-328082-7c7slfbp 308 16 preventing prevent VBG cord-328082-7c7slfbp 308 17 cells cell NNS cord-328082-7c7slfbp 308 18 in in IN cord-328082-7c7slfbp 308 19 the the DT cord-328082-7c7slfbp 308 20 S s NN cord-328082-7c7slfbp 308 21 - - HYPH cord-328082-7c7slfbp 308 22 phase phase NN cord-328082-7c7slfbp 308 23 to to TO cord-328082-7c7slfbp 308 24 proceed proceed VB cord-328082-7c7slfbp 308 25 in in IN cord-328082-7c7slfbp 308 26 the the DT cord-328082-7c7slfbp 308 27 mitotic mitotic JJ cord-328082-7c7slfbp 308 28 cycle cycle NN cord-328082-7c7slfbp 308 29 ( ( -LRB- cord-328082-7c7slfbp 308 30 Schlegel Schlegel NNP cord-328082-7c7slfbp 308 31 and and CC cord-328082-7c7slfbp 308 32 Pardee Pardee NNP cord-328082-7c7slfbp 308 33 , , , cord-328082-7c7slfbp 308 34 1986 1986 CD cord-328082-7c7slfbp 308 35 ; ; : cord-328082-7c7slfbp 308 36 Downes Downes NNP cord-328082-7c7slfbp 308 37 et et NNP cord-328082-7c7slfbp 308 38 al al NNP cord-328082-7c7slfbp 308 39 . . NNP cord-328082-7c7slfbp 308 40 , , , cord-328082-7c7slfbp 308 41 1990 1990 CD cord-328082-7c7slfbp 308 42 ) ) -RRB- cord-328082-7c7slfbp 308 43 , , , cord-328082-7c7slfbp 308 44 and and CC cord-328082-7c7slfbp 308 45 , , , cord-328082-7c7slfbp 308 46 on on IN cord-328082-7c7slfbp 308 47 the the DT cord-328082-7c7slfbp 308 48 other other JJ cord-328082-7c7slfbp 308 49 hand hand NN cord-328082-7c7slfbp 308 50 , , , cord-328082-7c7slfbp 308 51 the the DT cord-328082-7c7slfbp 308 52 onset onset NN cord-328082-7c7slfbp 308 53 of of IN cord-328082-7c7slfbp 308 54 mitosis mitosis NN cord-328082-7c7slfbp 308 55 involves involve VBZ cord-328082-7c7slfbp 308 56 complex complex JJ cord-328082-7c7slfbp 308 57 cascades cascade NNS cord-328082-7c7slfbp 308 58 of of IN cord-328082-7c7slfbp 308 59 phosphorylations phosphorylation NNS cord-328082-7c7slfbp 308 60 and and CC cord-328082-7c7slfbp 308 61 dephosphorylations dephosphorylation NNS cord-328082-7c7slfbp 308 62 controlling control VBG cord-328082-7c7slfbp 308 63 the the DT cord-328082-7c7slfbp 308 64 progress progress NN cord-328082-7c7slfbp 308 65 of of IN cord-328082-7c7slfbp 308 66 cell cell NN cord-328082-7c7slfbp 308 67 division division NN cord-328082-7c7slfbp 308 68 ( ( -LRB- cord-328082-7c7slfbp 308 69 Lewin Lewin NNP cord-328082-7c7slfbp 308 70 , , , cord-328082-7c7slfbp 308 71 1990 1990 CD cord-328082-7c7slfbp 308 72 ) ) -RRB- cord-328082-7c7slfbp 308 73 , , , cord-328082-7c7slfbp 308 74 it -PRON- PRP cord-328082-7c7slfbp 308 75 could could MD cord-328082-7c7slfbp 308 76 be be VB cord-328082-7c7slfbp 308 77 possible possible JJ cord-328082-7c7slfbp 308 78 that that IN cord-328082-7c7slfbp 308 79 caffeine caffeine NN cord-328082-7c7slfbp 308 80 acts act VBZ cord-328082-7c7slfbp 308 81 through through IN cord-328082-7c7slfbp 308 82 the the DT cord-328082-7c7slfbp 308 83 activation activation NN cord-328082-7c7slfbp 308 84 or or CC cord-328082-7c7slfbp 308 85 deactivation deactivation NN cord-328082-7c7slfbp 308 86 of of IN cord-328082-7c7slfbp 308 87 some some DT cord-328082-7c7slfbp 308 88 kinase kinase NN cord-328082-7c7slfbp 308 89 or or CC cord-328082-7c7slfbp 308 90 phosphatase phosphatase NN cord-328082-7c7slfbp 308 91 . . . cord-328082-7c7slfbp 309 1 Also also RB cord-328082-7c7slfbp 309 2 , , , cord-328082-7c7slfbp 309 3 it -PRON- PRP cord-328082-7c7slfbp 309 4 has have VBZ cord-328082-7c7slfbp 309 5 recently recently RB cord-328082-7c7slfbp 309 6 been be VBN cord-328082-7c7slfbp 309 7 reported report VBN cord-328082-7c7slfbp 309 8 that that IN cord-328082-7c7slfbp 309 9 okadaic okadaic JJ cord-328082-7c7slfbp 309 10 acid acid NN cord-328082-7c7slfbp 309 11 , , , cord-328082-7c7slfbp 309 12 a a DT cord-328082-7c7slfbp 309 13 phosphatase phosphatase NN cord-328082-7c7slfbp 309 14 inhibitor inhibitor NN cord-328082-7c7slfbp 309 15 , , , cord-328082-7c7slfbp 309 16 affects affect VBZ cord-328082-7c7slfbp 309 17 the the DT cord-328082-7c7slfbp 309 18 ER ER NNP cord-328082-7c7slfbp 309 19 - - HYPH cord-328082-7c7slfbp 309 20 to to IN cord-328082-7c7slfbp 309 21 - - HYPH cord-328082-7c7slfbp 309 22 Golgi golgi NN cord-328082-7c7slfbp 309 23 traffic traffic NN cord-328082-7c7slfbp 309 24 ( ( -LRB- cord-328082-7c7slfbp 309 25 Davidson Davidson NNP cord-328082-7c7slfbp 309 26 et et FW cord-328082-7c7slfbp 309 27 al al NNP cord-328082-7c7slfbp 309 28 . . NNP cord-328082-7c7slfbp 309 29 , , , cord-328082-7c7slfbp 309 30 1992 1992 CD cord-328082-7c7slfbp 309 31 ) ) -RRB- cord-328082-7c7slfbp 309 32 and and CC cord-328082-7c7slfbp 309 33 the the DT cord-328082-7c7slfbp 309 34 morphology morphology NN cord-328082-7c7slfbp 309 35 of of IN cord-328082-7c7slfbp 309 36 the the DT cord-328082-7c7slfbp 309 37 Golgi Golgi NNP cord-328082-7c7slfbp 309 38 apparatus apparatus NN cord-328082-7c7slfbp 309 39 ( ( -LRB- cord-328082-7c7slfbp 309 40 Lucocq Lucocq NNP cord-328082-7c7slfbp 309 41 et et FW cord-328082-7c7slfbp 309 42 al al NNP cord-328082-7c7slfbp 309 43 . . NNP cord-328082-7c7slfbp 309 44 , , , cord-328082-7c7slfbp 309 45 1991 1991 CD cord-328082-7c7slfbp 309 46 ) ) -RRB- cord-328082-7c7slfbp 309 47 . . . cord-328082-7c7slfbp 310 1 In in IN cord-328082-7c7slfbp 310 2 the the DT cord-328082-7c7slfbp 310 3 present present JJ cord-328082-7c7slfbp 310 4 study study NN cord-328082-7c7slfbp 310 5 , , , cord-328082-7c7slfbp 310 6 we -PRON- PRP cord-328082-7c7slfbp 310 7 have have VBP cord-328082-7c7slfbp 310 8 dissected dissect VBN cord-328082-7c7slfbp 310 9 the the DT cord-328082-7c7slfbp 310 10 compartmental compartmental JJ cord-328082-7c7slfbp 310 11 organization organization NN cord-328082-7c7slfbp 310 12 of of IN cord-328082-7c7slfbp 310 13 the the DT cord-328082-7c7slfbp 310 14 ER ER NNP cord-328082-7c7slfbp 310 15 - - HYPH cord-328082-7c7slfbp 310 16 to to IN cord-328082-7c7slfbp 310 17 - - HYPH cord-328082-7c7slfbp 310 18 Golgi golgi NN cord-328082-7c7slfbp 310 19 interface interface NN cord-328082-7c7slfbp 310 20 using use VBG cord-328082-7c7slfbp 310 21 the the DT cord-328082-7c7slfbp 310 22 transport transport NN cord-328082-7c7slfbp 310 23 inhibition inhibition NN cord-328082-7c7slfbp 310 24 exerted exert VBN cord-328082-7c7slfbp 310 25 by by IN cord-328082-7c7slfbp 310 26 10 10 CD cord-328082-7c7slfbp 310 27 mM mm CD cord-328082-7c7slfbp 310 28 caffeine caffeine NN cord-328082-7c7slfbp 310 29 at at IN cord-328082-7c7slfbp 310 30 20"C 20"c CD cord-328082-7c7slfbp 310 31 . . . cord-328082-7c7slfbp 311 1 The the DT cord-328082-7c7slfbp 311 2 temperature temperature NN cord-328082-7c7slfbp 311 3 threshold threshold NN cord-328082-7c7slfbp 311 4 inhibiting inhibit VBG cord-328082-7c7slfbp 311 5 the the DT cord-328082-7c7slfbp 311 6 exit exit NN cord-328082-7c7slfbp 311 7 of of IN cord-328082-7c7slfbp 311 8 SFV sfv NN cord-328082-7c7slfbp 311 9 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 311 10 from from IN cord-328082-7c7slfbp 311 11 the the DT cord-328082-7c7slfbp 311 12 ER ER NNP cord-328082-7c7slfbp 311 13 was be VBD cord-328082-7c7slfbp 311 14 found find VBN cord-328082-7c7slfbp 311 15 to to TO cord-328082-7c7slfbp 311 16 be be VB cord-328082-7c7slfbp 311 17 23 23 CD cord-328082-7c7slfbp 311 18 - - SYM cord-328082-7c7slfbp 311 19 24~ 24~ CD cord-328082-7c7slfbp 312 1 At at IN cord-328082-7c7slfbp 312 2 25~ 25~ CD cord-328082-7c7slfbp 312 3 SFV sfv NN cord-328082-7c7slfbp 312 4 E1 e1 NN cord-328082-7c7slfbp 312 5 reached reach VBD cord-328082-7c7slfbp 312 6 the the DT cord-328082-7c7slfbp 312 7 Golgi Golgi NNP cord-328082-7c7slfbp 312 8 stack stack NN cord-328082-7c7slfbp 312 9 as as IN cord-328082-7c7slfbp 312 10 indicated indicate VBN cord-328082-7c7slfbp 312 11 by by IN cord-328082-7c7slfbp 312 12 the the DT cord-328082-7c7slfbp 312 13 Endo Endo NNP cord-328082-7c7slfbp 312 14 H H NNP cord-328082-7c7slfbp 312 15 analysis analysis NN cord-328082-7c7slfbp 312 16 . . . cord-328082-7c7slfbp 313 1 Some some DT cord-328082-7c7slfbp 313 2 variation variation NN cord-328082-7c7slfbp 313 3 , , , cord-328082-7c7slfbp 313 4 however however RB cord-328082-7c7slfbp 313 5 , , , cord-328082-7c7slfbp 313 6 was be VBD cord-328082-7c7slfbp 313 7 observed observe VBN cord-328082-7c7slfbp 313 8 in in IN cord-328082-7c7slfbp 313 9 the the DT cord-328082-7c7slfbp 313 10 temperature temperature NN cord-328082-7c7slfbp 313 11 dependence dependence NN cord-328082-7c7slfbp 313 12 between between IN cord-328082-7c7slfbp 313 13 individual individual JJ cord-328082-7c7slfbp 313 14 cells cell NNS cord-328082-7c7slfbp 313 15 close close RB cord-328082-7c7slfbp 313 16 to to IN cord-328082-7c7slfbp 313 17 the the DT cord-328082-7c7slfbp 313 18 threshold threshold NN cord-328082-7c7slfbp 313 19 temperature temperature NN cord-328082-7c7slfbp 313 20 . . . cord-328082-7c7slfbp 314 1 Therefore therefore RB cord-328082-7c7slfbp 314 2 , , , cord-328082-7c7slfbp 314 3 in in IN cord-328082-7c7slfbp 314 4 other other JJ cord-328082-7c7slfbp 314 5 experiments experiment NNS cord-328082-7c7slfbp 314 6 , , , cord-328082-7c7slfbp 314 7 20~ 20~ CD cord-328082-7c7slfbp 314 8 in in IN cord-328082-7c7slfbp 314 9 the the DT cord-328082-7c7slfbp 314 10 presence presence NN cord-328082-7c7slfbp 314 11 of of IN cord-328082-7c7slfbp 314 12 caffeine caffeine NN cord-328082-7c7slfbp 314 13 was be VBD cord-328082-7c7slfbp 314 14 used use VBN cord-328082-7c7slfbp 314 15 . . . cord-328082-7c7slfbp 315 1 To to TO cord-328082-7c7slfbp 315 2 study study VB cord-328082-7c7slfbp 315 3 whether whether IN cord-328082-7c7slfbp 315 4 the the DT cord-328082-7c7slfbp 315 5 transport transport NN cord-328082-7c7slfbp 315 6 of of IN cord-328082-7c7slfbp 315 7 cellular cellular JJ cord-328082-7c7slfbp 315 8 proteins protein NNS cord-328082-7c7slfbp 315 9 is be VBZ cord-328082-7c7slfbp 315 10 also also RB cord-328082-7c7slfbp 315 11 affected affect VBN cord-328082-7c7slfbp 315 12 , , , cord-328082-7c7slfbp 315 13 man man NN cord-328082-7c7slfbp 315 14 II II NNP cord-328082-7c7slfbp 315 15 was be VBD cord-328082-7c7slfbp 315 16 translocated translocate VBN cord-328082-7c7slfbp 315 17 with with IN cord-328082-7c7slfbp 315 18 BFA BFA NNP cord-328082-7c7slfbp 315 19 to to IN cord-328082-7c7slfbp 315 20 the the DT cord-328082-7c7slfbp 315 21 ER ER NNP cord-328082-7c7slfbp 315 22 , , , cord-328082-7c7slfbp 315 23 whereafter whereaft JJR cord-328082-7c7slfbp 315 24 BFA BFA NNP cord-328082-7c7slfbp 315 25 was be VBD cord-328082-7c7slfbp 315 26 washed wash VBN cord-328082-7c7slfbp 315 27 away away RB cord-328082-7c7slfbp 315 28 and and CC cord-328082-7c7slfbp 315 29 the the DT cord-328082-7c7slfbp 315 30 movement movement NN cord-328082-7c7slfbp 315 31 of of IN cord-328082-7c7slfbp 315 32 man man NNP cord-328082-7c7slfbp 315 33 II II NNP cord-328082-7c7slfbp 315 34 was be VBD cord-328082-7c7slfbp 315 35 followed follow VBN cord-328082-7c7slfbp 315 36 in in IN cord-328082-7c7slfbp 315 37 the the DT cord-328082-7c7slfbp 315 38 absence absence NN cord-328082-7c7slfbp 315 39 or or CC cord-328082-7c7slfbp 315 40 presence presence NN cord-328082-7c7slfbp 315 41 of of IN cord-328082-7c7slfbp 315 42 10 10 CD cord-328082-7c7slfbp 315 43 mM mm CD cord-328082-7c7slfbp 315 44 caffeine caffeine NN cord-328082-7c7slfbp 315 45 at at IN cord-328082-7c7slfbp 315 46 20~ 20~ CD cord-328082-7c7slfbp 316 1 As as IN cord-328082-7c7slfbp 316 2 was be VBD cord-328082-7c7slfbp 316 3 the the DT cord-328082-7c7slfbp 316 4 case case NN cord-328082-7c7slfbp 316 5 with with IN cord-328082-7c7slfbp 316 6 virus virus NN cord-328082-7c7slfbp 316 7 proteins protein NNS cord-328082-7c7slfbp 316 8 , , , cord-328082-7c7slfbp 316 9 man man NN cord-328082-7c7slfbp 316 10 II II NNP cord-328082-7c7slfbp 316 11 was be VBD cord-328082-7c7slfbp 316 12 unable unable JJ cord-328082-7c7slfbp 316 13 to to TO cord-328082-7c7slfbp 316 14 exit exit VB cord-328082-7c7slfbp 316 15 from from IN cord-328082-7c7slfbp 316 16 the the DT cord-328082-7c7slfbp 316 17 ER ER NNP cord-328082-7c7slfbp 316 18 in in IN cord-328082-7c7slfbp 316 19 the the DT cord-328082-7c7slfbp 316 20 presence presence NN cord-328082-7c7slfbp 316 21 of of IN cord-328082-7c7slfbp 316 22 10 10 CD cord-328082-7c7slfbp 316 23 mM mm CD cord-328082-7c7slfbp 316 24 caffeine caffeine NN cord-328082-7c7slfbp 316 25 at at IN cord-328082-7c7slfbp 316 26 20~ 20~ CD cord-328082-7c7slfbp 316 27 during during IN cord-328082-7c7slfbp 316 28 a a DT cord-328082-7c7slfbp 316 29 180-min 180-min CD cord-328082-7c7slfbp 316 30 chase chase NN cord-328082-7c7slfbp 316 31 . . . cord-328082-7c7slfbp 317 1 In in IN cord-328082-7c7slfbp 317 2 contrast contrast NN cord-328082-7c7slfbp 317 3 , , , cord-328082-7c7slfbp 317 4 during during IN cord-328082-7c7slfbp 317 5 a a DT cord-328082-7c7slfbp 317 6 180-min 180-min CD cord-328082-7c7slfbp 317 7 chase chase NN cord-328082-7c7slfbp 317 8 in in IN cord-328082-7c7slfbp 317 9 the the DT cord-328082-7c7slfbp 317 10 control control NN cord-328082-7c7slfbp 317 11 cells cell NNS cord-328082-7c7slfbp 317 12 , , , cord-328082-7c7slfbp 317 13 ER ER NNP cord-328082-7c7slfbp 317 14 was be VBD cord-328082-7c7slfbp 317 15 efficiently efficiently RB cord-328082-7c7slfbp 317 16 emptied empty VBN cord-328082-7c7slfbp 317 17 and and CC cord-328082-7c7slfbp 317 18 man man NN cord-328082-7c7slfbp 317 19 II ii CD cord-328082-7c7slfbp 317 20 accumulated accumulate VBN cord-328082-7c7slfbp 317 21 perinuclearly perinuclearly RB cord-328082-7c7slfbp 317 22 in in IN cord-328082-7c7slfbp 317 23 the the DT cord-328082-7c7slfbp 317 24 absence absence NN cord-328082-7c7slfbp 317 25 of of IN cord-328082-7c7slfbp 317 26 caffeine caffeine NN cord-328082-7c7slfbp 317 27 at at IN cord-328082-7c7slfbp 317 28 20~ 20~ CD cord-328082-7c7slfbp 318 1 It -PRON- PRP cord-328082-7c7slfbp 318 2 is be VBZ cord-328082-7c7slfbp 318 3 therefore therefore RB cord-328082-7c7slfbp 318 4 suggested suggest VBN cord-328082-7c7slfbp 318 5 that that IN cord-328082-7c7slfbp 318 6 caffeine caffeine NN cord-328082-7c7slfbp 318 7 affects affect VBZ cord-328082-7c7slfbp 318 8 the the DT cord-328082-7c7slfbp 318 9 movement movement NN cord-328082-7c7slfbp 318 10 of of IN cord-328082-7c7slfbp 318 11 membranes membrane NNS cord-328082-7c7slfbp 318 12 between between IN cord-328082-7c7slfbp 318 13 the the DT cord-328082-7c7slfbp 318 14 ER ER NNP cord-328082-7c7slfbp 318 15 and and CC cord-328082-7c7slfbp 318 16 the the DT cord-328082-7c7slfbp 318 17 Golgi Golgi NNP cord-328082-7c7slfbp 318 18 complex complex NN cord-328082-7c7slfbp 318 19 . . . cord-328082-7c7slfbp 319 1 It -PRON- PRP cord-328082-7c7slfbp 319 2 is be VBZ cord-328082-7c7slfbp 319 3 , , , cord-328082-7c7slfbp 319 4 however however RB cord-328082-7c7slfbp 319 5 , , , cord-328082-7c7slfbp 319 6 necessary necessary JJ cord-328082-7c7slfbp 319 7 to to TO cord-328082-7c7slfbp 319 8 follow follow VB cord-328082-7c7slfbp 319 9 the the DT cord-328082-7c7slfbp 319 10 movement movement NN cord-328082-7c7slfbp 319 11 of of IN cord-328082-7c7slfbp 319 12 other other JJ cord-328082-7c7slfbp 319 13 cellular cellular JJ cord-328082-7c7slfbp 319 14 proteins protein NNS cord-328082-7c7slfbp 319 15 and and CC cord-328082-7c7slfbp 319 16 , , , cord-328082-7c7slfbp 319 17 for for IN cord-328082-7c7slfbp 319 18 example example NN cord-328082-7c7slfbp 319 19 , , , cord-328082-7c7slfbp 319 20 lipids lipid NNS cord-328082-7c7slfbp 319 21 between between IN cord-328082-7c7slfbp 319 22 the the DT cord-328082-7c7slfbp 319 23 ER ER NNP cord-328082-7c7slfbp 319 24 and and CC cord-328082-7c7slfbp 319 25 the the DT cord-328082-7c7slfbp 319 26 Golgi Golgi NNP cord-328082-7c7slfbp 319 27 in in IN cord-328082-7c7slfbp 319 28 the the DT cord-328082-7c7slfbp 319 29 presence presence NN cord-328082-7c7slfbp 319 30 of of IN cord-328082-7c7slfbp 319 31 caffeine caffeine NN cord-328082-7c7slfbp 319 32 at at IN cord-328082-7c7slfbp 319 33 reduced reduce VBN cord-328082-7c7slfbp 319 34 temperature temperature NN cord-328082-7c7slfbp 319 35 to to TO cord-328082-7c7slfbp 319 36 obtain obtain VB cord-328082-7c7slfbp 319 37 a a DT cord-328082-7c7slfbp 319 38 clear clear JJ cord-328082-7c7slfbp 319 39 picture picture NN cord-328082-7c7slfbp 319 40 of of IN cord-328082-7c7slfbp 319 41 this this DT cord-328082-7c7slfbp 319 42 matter matter NN cord-328082-7c7slfbp 319 43 . . . cord-328082-7c7slfbp 320 1 We -PRON- PRP cord-328082-7c7slfbp 320 2 have have VBP cord-328082-7c7slfbp 320 3 also also RB cord-328082-7c7slfbp 320 4 tested test VBN cord-328082-7c7slfbp 320 5 that that IN cord-328082-7c7slfbp 320 6 the the DT cord-328082-7c7slfbp 320 7 ER er NN cord-328082-7c7slfbp 320 8 - - HYPH cord-328082-7c7slfbp 320 9 exit exit NN cord-328082-7c7slfbp 320 10 inhibition inhibition NN cord-328082-7c7slfbp 320 11 by by IN cord-328082-7c7slfbp 320 12 caffeine caffeine NN cord-328082-7c7slfbp 320 13 functions function NNS cord-328082-7c7slfbp 320 14 in in IN cord-328082-7c7slfbp 320 15 SFV sfv NN cord-328082-7c7slfbp 320 16 - - HYPH cord-328082-7c7slfbp 320 17 infected infect VBN cord-328082-7c7slfbp 320 18 normal normal JJ cord-328082-7c7slfbp 320 19 rat rat NN cord-328082-7c7slfbp 320 20 kidney kidney NN cord-328082-7c7slfbp 320 21 cells cell NNS cord-328082-7c7slfbp 320 22 ( ( -LRB- cord-328082-7c7slfbp 320 23 data datum NNS cord-328082-7c7slfbp 320 24 not not RB cord-328082-7c7slfbp 320 25 shown show VBN cord-328082-7c7slfbp 320 26 ) ) -RRB- cord-328082-7c7slfbp 320 27 , , , cord-328082-7c7slfbp 320 28 indicating indicate VBG cord-328082-7c7slfbp 320 29 that that IN cord-328082-7c7slfbp 320 30 the the DT cord-328082-7c7slfbp 320 31 observed observed JJ cord-328082-7c7slfbp 320 32 effect effect NN cord-328082-7c7slfbp 320 33 is be VBZ cord-328082-7c7slfbp 320 34 not not RB cord-328082-7c7slfbp 320 35 specific specific JJ cord-328082-7c7slfbp 320 36 for for IN cord-328082-7c7slfbp 320 37 one one CD cord-328082-7c7slfbp 320 38 cell cell NN cord-328082-7c7slfbp 320 39 type type NN cord-328082-7c7slfbp 320 40 . . . cord-328082-7c7slfbp 321 1 An an DT cord-328082-7c7slfbp 321 2 interesting interesting JJ cord-328082-7c7slfbp 321 3 question question NN cord-328082-7c7slfbp 321 4 was be VBD cord-328082-7c7slfbp 321 5 whether whether IN cord-328082-7c7slfbp 321 6 caffeine caffeine NN cord-328082-7c7slfbp 321 7 and and CC cord-328082-7c7slfbp 321 8 reduced reduce VBN cord-328082-7c7slfbp 321 9 temperature temperature NN cord-328082-7c7slfbp 321 10 would would MD cord-328082-7c7slfbp 321 11 inhibit inhibit VB cord-328082-7c7slfbp 321 12 also also RB cord-328082-7c7slfbp 321 13 the the DT cord-328082-7c7slfbp 321 14 BFA BFA NNP cord-328082-7c7slfbp 321 15 - - HYPH cord-328082-7c7slfbp 321 16 induced induce VBN cord-328082-7c7slfbp 321 17 retrograde retrograde JJ cord-328082-7c7slfbp 321 18 movement movement NN cord-328082-7c7slfbp 321 19 of of IN cord-328082-7c7slfbp 321 20 the the DT cord-328082-7c7slfbp 321 21 Golgi Golgi NNP cord-328082-7c7slfbp 321 22 membranes membrane NNS cord-328082-7c7slfbp 321 23 . . . cord-328082-7c7slfbp 322 1 In in IN cord-328082-7c7slfbp 322 2 the the DT cord-328082-7c7slfbp 322 3 presence presence NN cord-328082-7c7slfbp 322 4 of of IN cord-328082-7c7slfbp 322 5 10 10 CD cord-328082-7c7slfbp 322 6 mM mm CD cord-328082-7c7slfbp 322 7 caffeine caffeine NN cord-328082-7c7slfbp 322 8 at at IN cord-328082-7c7slfbp 322 9 20~ 20~ CD cord-328082-7c7slfbp 322 10 BFA BFA NNP cord-328082-7c7slfbp 322 11 was be VBD cord-328082-7c7slfbp 322 12 able able JJ cord-328082-7c7slfbp 322 13 to to TO cord-328082-7c7slfbp 322 14 redistribute redistribute VB cord-328082-7c7slfbp 322 15 at at IN cord-328082-7c7slfbp 322 16 least least JJS cord-328082-7c7slfbp 322 17 part part NN cord-328082-7c7slfbp 322 18 of of IN cord-328082-7c7slfbp 322 19 the the DT cord-328082-7c7slfbp 322 20 cellular cellular JJ cord-328082-7c7slfbp 322 21 man man NN cord-328082-7c7slfbp 322 22 II II NNP cord-328082-7c7slfbp 322 23 to to IN cord-328082-7c7slfbp 322 24 the the DT cord-328082-7c7slfbp 322 25 ER ER NNP cord-328082-7c7slfbp 322 26 with with IN cord-328082-7c7slfbp 322 27 nearly nearly RB cord-328082-7c7slfbp 322 28 similar similar JJ cord-328082-7c7slfbp 322 29 kinetics kinetic NNS cord-328082-7c7slfbp 322 30 than than IN cord-328082-7c7slfbp 322 31 with with IN cord-328082-7c7slfbp 322 32 BFA BFA NNP cord-328082-7c7slfbp 322 33 only only RB cord-328082-7c7slfbp 322 34 at at IN cord-328082-7c7slfbp 322 35 20~ 20~ CD cord-328082-7c7slfbp 323 1 Interestingly interestingly RB cord-328082-7c7slfbp 323 2 , , , cord-328082-7c7slfbp 323 3 caffeine caffeine NN cord-328082-7c7slfbp 323 4 did do VBD cord-328082-7c7slfbp 323 5 seem seem VB cord-328082-7c7slfbp 323 6 to to TO cord-328082-7c7slfbp 323 7 slow slow VB cord-328082-7c7slfbp 323 8 down down RP cord-328082-7c7slfbp 323 9 the the DT cord-328082-7c7slfbp 323 10 rate rate NN cord-328082-7c7slfbp 323 11 by by IN cord-328082-7c7slfbp 323 12 which which WDT cord-328082-7c7slfbp 323 13 BFA BFA NNP cord-328082-7c7slfbp 323 14 treatment treatment NN cord-328082-7c7slfbp 323 15 results result NNS cord-328082-7c7slfbp 323 16 in in IN cord-328082-7c7slfbp 323 17 the the DT cord-328082-7c7slfbp 323 18 disappearance disappearance NN cord-328082-7c7slfbp 323 19 of of IN cord-328082-7c7slfbp 323 20 any any DT cord-328082-7c7slfbp 323 21 recognizable recognizable JJ cord-328082-7c7slfbp 323 22 Golgi Golgi NNP cord-328082-7c7slfbp 323 23 complex complex NN cord-328082-7c7slfbp 323 24 by by IN cord-328082-7c7slfbp 323 25 a a DT cord-328082-7c7slfbp 323 26 factor factor NN cord-328082-7c7slfbp 323 27 of of IN cord-328082-7c7slfbp 323 28 ~1.6 ~1.6 CD cord-328082-7c7slfbp 323 29 . . . cord-328082-7c7slfbp 324 1 Nevertheless nevertheless RB cord-328082-7c7slfbp 324 2 , , , cord-328082-7c7slfbp 324 3 already already RB cord-328082-7c7slfbp 324 4 after after IN cord-328082-7c7slfbp 324 5 a a DT cord-328082-7c7slfbp 324 6 60-min 60-min CD cord-328082-7c7slfbp 324 7 chase chase NN cord-328082-7c7slfbp 324 8 in in IN cord-328082-7c7slfbp 324 9 the the DT cord-328082-7c7slfbp 324 10 presence presence NN cord-328082-7c7slfbp 324 11 of of IN cord-328082-7c7slfbp 324 12 caffeine caffeine NN cord-328082-7c7slfbp 324 13 at at IN cord-328082-7c7slfbp 324 14 20~ 20~ CD cord-328082-7c7slfbp 324 15 68 68 CD cord-328082-7c7slfbp 324 16 % % NN cord-328082-7c7slfbp 324 17 of of IN cord-328082-7c7slfbp 324 18 the the DT cord-328082-7c7slfbp 324 19 cells cell NNS cord-328082-7c7slfbp 324 20 showed show VBD cord-328082-7c7slfbp 324 21 labeling label VBG cord-328082-7c7slfbp 324 22 in in IN cord-328082-7c7slfbp 324 23 the the DT cord-328082-7c7slfbp 324 24 ER er NN cord-328082-7c7slfbp 324 25 and and CC cord-328082-7c7slfbp 324 26 long long JJ cord-328082-7c7slfbp 324 27 tubular tubular JJ cord-328082-7c7slfbp 324 28 structures structure NNS cord-328082-7c7slfbp 324 29 emanating emanate VBG cord-328082-7c7slfbp 324 30 from from IN cord-328082-7c7slfbp 324 31 the the DT cord-328082-7c7slfbp 324 32 Golgi Golgi NNP cord-328082-7c7slfbp 324 33 complex complex NN cord-328082-7c7slfbp 324 34 . . . cord-328082-7c7slfbp 325 1 These these DT cord-328082-7c7slfbp 325 2 results result NNS cord-328082-7c7slfbp 325 3 show show VBP cord-328082-7c7slfbp 325 4 that that IN cord-328082-7c7slfbp 325 5 caffeine caffeine NN cord-328082-7c7slfbp 325 6 does do VBZ cord-328082-7c7slfbp 325 7 not not RB cord-328082-7c7slfbp 325 8 inhibit inhibit VB cord-328082-7c7slfbp 325 9 the the DT cord-328082-7c7slfbp 325 10 BFA BFA NNP cord-328082-7c7slfbp 325 11 - - HYPH cord-328082-7c7slfbp 325 12 induced induce VBN cord-328082-7c7slfbp 325 13 retrograde retrograde JJ cord-328082-7c7slfbp 325 14 Golgi Golgi NNP cord-328082-7c7slfbp 325 15 - - HYPH cord-328082-7c7slfbp 325 16 to to IN cord-328082-7c7slfbp 325 17 - - HYPH cord-328082-7c7slfbp 325 18 ER er NN cord-328082-7c7slfbp 325 19 transport transport NN cord-328082-7c7slfbp 325 20 , , , cord-328082-7c7slfbp 325 21 although although IN cord-328082-7c7slfbp 325 22 the the DT cord-328082-7c7slfbp 325 23 traffic traffic NN cord-328082-7c7slfbp 325 24 in in IN cord-328082-7c7slfbp 325 25 the the DT cord-328082-7c7slfbp 325 26 opposite opposite NN cord-328082-7c7slfbp 325 27 ( ( -LRB- cord-328082-7c7slfbp 325 28 ER er NN cord-328082-7c7slfbp 325 29 - - HYPH cord-328082-7c7slfbp 325 30 to to IN cord-328082-7c7slfbp 325 31 - - HYPH cord-328082-7c7slfbp 325 32 Golgi golgi NN cord-328082-7c7slfbp 325 33 ) ) -RRB- cord-328082-7c7slfbp 325 34 direction direction NN cord-328082-7c7slfbp 325 35 , , , cord-328082-7c7slfbp 325 36 is be VBZ cord-328082-7c7slfbp 325 37 inhibited inhibit VBN cord-328082-7c7slfbp 325 38 . . . cord-328082-7c7slfbp 326 1 Also also RB cord-328082-7c7slfbp 326 2 , , , cord-328082-7c7slfbp 326 3 since since IN cord-328082-7c7slfbp 326 4 caffeine caffeine NN cord-328082-7c7slfbp 326 5 at at IN cord-328082-7c7slfbp 326 6 20~ 20~ CD cord-328082-7c7slfbp 326 7 did do VBD cord-328082-7c7slfbp 326 8 not not RB cord-328082-7c7slfbp 326 9 inhibit inhibit VB cord-328082-7c7slfbp 326 10 the the DT cord-328082-7c7slfbp 326 11 BFA BFA NNP cord-328082-7c7slfbp 326 12 - - HYPH cord-328082-7c7slfbp 326 13 induced induce VBN cord-328082-7c7slfbp 326 14 retrograde retrograde JJ cord-328082-7c7slfbp 326 15 movement movement NN cord-328082-7c7slfbp 326 16 of of IN cord-328082-7c7slfbp 326 17 Golgi Golgi NNP cord-328082-7c7slfbp 326 18 membranes membrane NNS cord-328082-7c7slfbp 326 19 , , , cord-328082-7c7slfbp 326 20 known know VBN cord-328082-7c7slfbp 326 21 to to TO cord-328082-7c7slfbp 326 22 be be VB cord-328082-7c7slfbp 326 23 inhibited inhibit VBN cord-328082-7c7slfbp 326 24 by by IN cord-328082-7c7slfbp 326 25 the the DT cord-328082-7c7slfbp 326 26 GTP-,y GTP-,y NNP cord-328082-7c7slfbp 326 27 - - HYPH cord-328082-7c7slfbp 326 28 S S NNP cord-328082-7c7slfbp 326 29 ( ( -LRB- cord-328082-7c7slfbp 326 30 Tan Tan NNP cord-328082-7c7slfbp 326 31 et et FW cord-328082-7c7slfbp 326 32 al al NNP cord-328082-7c7slfbp 326 33 . . NNP cord-328082-7c7slfbp 326 34 , , , cord-328082-7c7slfbp 326 35 1992 1992 CD cord-328082-7c7slfbp 326 36 ) ) -RRB- cord-328082-7c7slfbp 326 37 , , , cord-328082-7c7slfbp 326 38 it -PRON- PRP cord-328082-7c7slfbp 326 39 seems seem VBZ cord-328082-7c7slfbp 326 40 unlikely unlikely JJ cord-328082-7c7slfbp 326 41 that that IN cord-328082-7c7slfbp 326 42 the the DT cord-328082-7c7slfbp 326 43 effect effect NN cord-328082-7c7slfbp 326 44 of of IN cord-328082-7c7slfbp 326 45 caffeine caffeine NN cord-328082-7c7slfbp 326 46 would would MD cord-328082-7c7slfbp 326 47 be be VB cord-328082-7c7slfbp 326 48 due due JJ cord-328082-7c7slfbp 326 49 to to IN cord-328082-7c7slfbp 326 50 caffeine caffeine NN cord-328082-7c7slfbp 326 51 mimicking mimic VBG cord-328082-7c7slfbp 326 52 GTP-3,-S. GTP-3,-S. NNP cord-328082-7c7slfbp 326 53 Electron Electron NNP cord-328082-7c7slfbp 326 54 microscopy microscopy NN cord-328082-7c7slfbp 326 55 study study NN cord-328082-7c7slfbp 326 56 of of IN cord-328082-7c7slfbp 326 57 the the DT cord-328082-7c7slfbp 326 58 Golgi Golgi NNP cord-328082-7c7slfbp 326 59 structure structure NN cord-328082-7c7slfbp 326 60 revealed reveal VBD cord-328082-7c7slfbp 326 61 a a DT cord-328082-7c7slfbp 326 62 vesicularization vesicularization NN cord-328082-7c7slfbp 326 63 of of IN cord-328082-7c7slfbp 326 64 the the DT cord-328082-7c7slfbp 326 65 Golgi Golgi NNP cord-328082-7c7slfbp 326 66 stack stack NN cord-328082-7c7slfbp 326 67 in in IN cord-328082-7c7slfbp 326 68 the the DT cord-328082-7c7slfbp 326 69 presence presence NN cord-328082-7c7slfbp 326 70 of of IN cord-328082-7c7slfbp 326 71 10 10 CD cord-328082-7c7slfbp 326 72 mM mm CD cord-328082-7c7slfbp 326 73 caffeine caffeine NN cord-328082-7c7slfbp 326 74 at at IN cord-328082-7c7slfbp 326 75 20~ 20~ CD cord-328082-7c7slfbp 327 1 These these DT cord-328082-7c7slfbp 327 2 small small JJ cord-328082-7c7slfbp 327 3 vesicles vesicle NNS cord-328082-7c7slfbp 327 4 remained remain VBD cord-328082-7c7slfbp 327 5 as as IN cord-328082-7c7slfbp 327 6 tight tight JJ cord-328082-7c7slfbp 327 7 clusters cluster NNS cord-328082-7c7slfbp 327 8 at at IN cord-328082-7c7slfbp 327 9 the the DT cord-328082-7c7slfbp 327 10 perinuclear perinuclear NNP cord-328082-7c7slfbp 327 11 site site NN cord-328082-7c7slfbp 327 12 where where WRB cord-328082-7c7slfbp 327 13 the the DT cord-328082-7c7slfbp 327 14 Golgi Golgi NNP cord-328082-7c7slfbp 327 15 complex complex NN cord-328082-7c7slfbp 327 16 is be VBZ cord-328082-7c7slfbp 327 17 normally normally RB cord-328082-7c7slfbp 327 18 localized localize VBN cord-328082-7c7slfbp 327 19 . . . cord-328082-7c7slfbp 328 1 This this DT cord-328082-7c7slfbp 328 2 result result NN cord-328082-7c7slfbp 328 3 is be VBZ cord-328082-7c7slfbp 328 4 in in IN cord-328082-7c7slfbp 328 5 agreement agreement NN cord-328082-7c7slfbp 328 6 with with IN cord-328082-7c7slfbp 328 7 the the DT cord-328082-7c7slfbp 328 8 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 328 9 observations observation NNS cord-328082-7c7slfbp 328 10 with with IN cord-328082-7c7slfbp 328 11 man man NN cord-328082-7c7slfbp 328 12 II II NNP cord-328082-7c7slfbp 328 13 . . . cord-328082-7c7slfbp 329 1 Since since IN cord-328082-7c7slfbp 329 2 the the DT cord-328082-7c7slfbp 329 3 perinuclear perinuclear NNP cord-328082-7c7slfbp 329 4 localization localization NN cord-328082-7c7slfbp 329 5 of of IN cord-328082-7c7slfbp 329 6 the the DT cord-328082-7c7slfbp 329 7 Golgi Golgi NNP cord-328082-7c7slfbp 329 8 complex complex NN cord-328082-7c7slfbp 329 9 in in IN cord-328082-7c7slfbp 329 10 the the DT cord-328082-7c7slfbp 329 11 cell cell NN cord-328082-7c7slfbp 329 12 is be VBZ cord-328082-7c7slfbp 329 13 dependent dependent JJ cord-328082-7c7slfbp 329 14 on on IN cord-328082-7c7slfbp 329 15 the the DT cord-328082-7c7slfbp 329 16 integrity integrity NN cord-328082-7c7slfbp 329 17 of of IN cord-328082-7c7slfbp 329 18 the the DT cord-328082-7c7slfbp 329 19 microtubules microtubule NNS cord-328082-7c7slfbp 329 20 ( ( -LRB- cord-328082-7c7slfbp 329 21 Thyberg Thyberg NNP cord-328082-7c7slfbp 329 22 and and CC cord-328082-7c7slfbp 329 23 Moskalewski Moskalewski NNP cord-328082-7c7slfbp 329 24 , , , cord-328082-7c7slfbp 329 25 1985 1985 CD cord-328082-7c7slfbp 329 26 ; ; : cord-328082-7c7slfbp 329 27 Kreis Kreis NNP cord-328082-7c7slfbp 329 28 , , , cord-328082-7c7slfbp 329 29 1990 1990 CD cord-328082-7c7slfbp 329 30 ) ) -RRB- cord-328082-7c7slfbp 329 31 , , , cord-328082-7c7slfbp 329 32 it -PRON- PRP cord-328082-7c7slfbp 329 33 is be VBZ cord-328082-7c7slfbp 329 34 unlikely unlikely JJ cord-328082-7c7slfbp 329 35 that that IN cord-328082-7c7slfbp 329 36 caffeine caffeine NN cord-328082-7c7slfbp 329 37 at at IN cord-328082-7c7slfbp 329 38 20~ 20~ CD cord-328082-7c7slfbp 329 39 would would MD cord-328082-7c7slfbp 329 40 act act VB cord-328082-7c7slfbp 329 41 through through IN cord-328082-7c7slfbp 329 42 disrupting disrupt VBG cord-328082-7c7slfbp 329 43 the the DT cord-328082-7c7slfbp 329 44 microtubular microtubular NNP cord-328082-7c7slfbp 329 45 network network NN cord-328082-7c7slfbp 329 46 . . . cord-328082-7c7slfbp 330 1 This this DT cord-328082-7c7slfbp 330 2 is be VBZ cord-328082-7c7slfbp 330 3 further further RB cord-328082-7c7slfbp 330 4 supported support VBN cord-328082-7c7slfbp 330 5 by by IN cord-328082-7c7slfbp 330 6 the the DT cord-328082-7c7slfbp 330 7 fact fact NN cord-328082-7c7slfbp 330 8 that that IN cord-328082-7c7slfbp 330 9 also also RB cord-328082-7c7slfbp 330 10 in in IN cord-328082-7c7slfbp 330 11 caffeine caffeine NN cord-328082-7c7slfbp 330 12 - - HYPH cord-328082-7c7slfbp 330 13 treated treat VBN cord-328082-7c7slfbp 330 14 cells cell NNS cord-328082-7c7slfbp 330 15 the the DT cord-328082-7c7slfbp 330 16 BFA BFA NNP cord-328082-7c7slfbp 330 17 - - HYPH cord-328082-7c7slfbp 330 18 induced induce VBN cord-328082-7c7slfbp 330 19 tubular tubular JJ cord-328082-7c7slfbp 330 20 , , , cord-328082-7c7slfbp 330 21 microtubuledependent microtubuledependent JJ cord-328082-7c7slfbp 330 22 structures structure NNS cord-328082-7c7slfbp 330 23 were be VBD cord-328082-7c7slfbp 330 24 able able JJ cord-328082-7c7slfbp 330 25 to to TO cord-328082-7c7slfbp 330 26 form form VB cord-328082-7c7slfbp 330 27 . . . cord-328082-7c7slfbp 331 1 One one CD cord-328082-7c7slfbp 331 2 possible possible JJ cord-328082-7c7slfbp 331 3 explanation explanation NN cord-328082-7c7slfbp 331 4 for for IN cord-328082-7c7slfbp 331 5 the the DT cord-328082-7c7slfbp 331 6 caffeineinduced caffeineinduced JJ cord-328082-7c7slfbp 331 7 vesicularization vesicularization NN cord-328082-7c7slfbp 331 8 of of IN cord-328082-7c7slfbp 331 9 the the DT cord-328082-7c7slfbp 331 10 Golgi Golgi NNP cord-328082-7c7slfbp 331 11 stack stack NN cord-328082-7c7slfbp 331 12 could could MD cord-328082-7c7slfbp 331 13 be be VB cord-328082-7c7slfbp 331 14 that that IN cord-328082-7c7slfbp 331 15 when when WRB cord-328082-7c7slfbp 331 16 the the DT cord-328082-7c7slfbp 331 17 transport transport NN cord-328082-7c7slfbp 331 18 from from IN cord-328082-7c7slfbp 331 19 the the DT cord-328082-7c7slfbp 331 20 ER ER NNP cord-328082-7c7slfbp 331 21 to to IN cord-328082-7c7slfbp 331 22 the the DT cord-328082-7c7slfbp 331 23 Golgi Golgi NNP cord-328082-7c7slfbp 331 24 complex complex NN cord-328082-7c7slfbp 331 25 is be VBZ cord-328082-7c7slfbp 331 26 inhibited inhibit VBN cord-328082-7c7slfbp 331 27 , , , cord-328082-7c7slfbp 331 28 while while IN cord-328082-7c7slfbp 331 29 the the DT cord-328082-7c7slfbp 331 30 vesicular vesicular JJ cord-328082-7c7slfbp 331 31 recycling recycling NN cord-328082-7c7slfbp 331 32 in in IN cord-328082-7c7slfbp 331 33 the the DT cord-328082-7c7slfbp 331 34 Golgi Golgi NNP cord-328082-7c7slfbp 331 35 stack stack NN cord-328082-7c7slfbp 331 36 is be VBZ cord-328082-7c7slfbp 331 37 still still RB cord-328082-7c7slfbp 331 38 occurring occur VBG cord-328082-7c7slfbp 331 39 , , , cord-328082-7c7slfbp 331 40 the the DT cord-328082-7c7slfbp 331 41 Golgi Golgi NNP cord-328082-7c7slfbp 331 42 stack stack NN cord-328082-7c7slfbp 331 43 ultimately ultimately RB cord-328082-7c7slfbp 331 44 becomes become VBZ cord-328082-7c7slfbp 331 45 vesicularized vesicularized JJ cord-328082-7c7slfbp 331 46 . . . cord-328082-7c7slfbp 332 1 Localization localization NN cord-328082-7c7slfbp 332 2 of of IN cord-328082-7c7slfbp 332 3 marker marker NN cord-328082-7c7slfbp 332 4 proteins protein NNS cord-328082-7c7slfbp 332 5 of of IN cord-328082-7c7slfbp 332 6 the the DT cord-328082-7c7slfbp 332 7 pre pre JJ cord-328082-7c7slfbp 332 8 - - JJ cord-328082-7c7slfbp 332 9 Golgi golgi JJ cord-328082-7c7slfbp 332 10 membranes membrane NNS cord-328082-7c7slfbp 332 11 and and CC cord-328082-7c7slfbp 332 12 the the DT cord-328082-7c7slfbp 332 13 Golgi Golgi NNP cord-328082-7c7slfbp 332 14 stack stack NN cord-328082-7c7slfbp 332 15 demonstrated demonstrate VBD cord-328082-7c7slfbp 332 16 an an DT cord-328082-7c7slfbp 332 17 interesting interesting JJ cord-328082-7c7slfbp 332 18 , , , cord-328082-7c7slfbp 332 19 differential differential JJ cord-328082-7c7slfbp 332 20 effect effect NN cord-328082-7c7slfbp 332 21 of of IN cord-328082-7c7slfbp 332 22 caffeine caffeine NN cord-328082-7c7slfbp 332 23 on on IN cord-328082-7c7slfbp 332 24 the the DT cord-328082-7c7slfbp 332 25 distribution distribution NN cord-328082-7c7slfbp 332 26 of of IN cord-328082-7c7slfbp 332 27 these these DT cord-328082-7c7slfbp 332 28 membrane membrane NN cord-328082-7c7slfbp 332 29 compartments compartment NNS cord-328082-7c7slfbp 332 30 . . . cord-328082-7c7slfbp 333 1 The the DT cord-328082-7c7slfbp 333 2 p58-positive p58-positive JJ cord-328082-7c7slfbp 333 3 pre pre JJ cord-328082-7c7slfbp 333 4 - - JJ cord-328082-7c7slfbp 333 5 and and CC cord-328082-7c7slfbp 333 6 cis cis NNP cord-328082-7c7slfbp 333 7 - - HYPH cord-328082-7c7slfbp 333 8 Golgi Golgi NNP cord-328082-7c7slfbp 333 9 membranes membrane NNS cord-328082-7c7slfbp 333 10 lost lose VBD cord-328082-7c7slfbp 333 11 their -PRON- PRP$ cord-328082-7c7slfbp 333 12 normal normal JJ cord-328082-7c7slfbp 333 13 perinuclear perinuclear NN cord-328082-7c7slfbp 333 14 localization localization NN cord-328082-7c7slfbp 333 15 while while IN cord-328082-7c7slfbp 333 16 the the DT cord-328082-7c7slfbp 333 17 localization localization NN cord-328082-7c7slfbp 333 18 of of IN cord-328082-7c7slfbp 333 19 a a DT cord-328082-7c7slfbp 333 20 Golgi Golgi NNP cord-328082-7c7slfbp 333 21 stack stack NN cord-328082-7c7slfbp 333 22 protein protein NN cord-328082-7c7slfbp 333 23 , , , cord-328082-7c7slfbp 333 24 man man NN cord-328082-7c7slfbp 333 25 II II NNP cord-328082-7c7slfbp 333 26 , , , cord-328082-7c7slfbp 333 27 remained remain VBD cord-328082-7c7slfbp 333 28 unaffected unaffected JJ cord-328082-7c7slfbp 333 29 . . . cord-328082-7c7slfbp 334 1 The the DT cord-328082-7c7slfbp 334 2 normal normal JJ cord-328082-7c7slfbp 334 3 distribution distribution NN cord-328082-7c7slfbp 334 4 of of IN cord-328082-7c7slfbp 334 5 p58 p58 NN cord-328082-7c7slfbp 334 6 was be VBD cord-328082-7c7slfbp 334 7 restored restore VBN cord-328082-7c7slfbp 334 8 when when WRB cord-328082-7c7slfbp 334 9 caffeine caffeine NN cord-328082-7c7slfbp 334 10 was be VBD cord-328082-7c7slfbp 334 11 removed remove VBN cord-328082-7c7slfbp 334 12 . . . cord-328082-7c7slfbp 335 1 When when WRB cord-328082-7c7slfbp 335 2 the the DT cord-328082-7c7slfbp 335 3 localization localization NN cord-328082-7c7slfbp 335 4 of of IN cord-328082-7c7slfbp 335 5 p58 p58 NNP cord-328082-7c7slfbp 335 6 was be VBD cord-328082-7c7slfbp 335 7 studied study VBN cord-328082-7c7slfbp 335 8 with with IN cord-328082-7c7slfbp 335 9 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 335 10 microscopy microscopy NN cord-328082-7c7slfbp 335 11 in in IN cord-328082-7c7slfbp 335 12 caffeine caffeine NN cord-328082-7c7slfbp 335 13 - - HYPH cord-328082-7c7slfbp 335 14 treated treat VBN cord-328082-7c7slfbp 335 15 cells cell NNS cord-328082-7c7slfbp 335 16 at at IN cord-328082-7c7slfbp 335 17 20~ 20~ CD cord-328082-7c7slfbp 335 18 the the DT cord-328082-7c7slfbp 335 19 typical typical JJ cord-328082-7c7slfbp 335 20 tubulovesicular tubulovesicular JJ cord-328082-7c7slfbp 335 21 p58-positive p58-positive JJ cord-328082-7c7slfbp 335 22 elements element NNS cord-328082-7c7slfbp 335 23 ( ( -LRB- cord-328082-7c7slfbp 335 24 Saraste Saraste NNP cord-328082-7c7slfbp 335 25 and and CC cord-328082-7c7slfbp 335 26 Svensson Svensson NNP cord-328082-7c7slfbp 335 27 , , , cord-328082-7c7slfbp 335 28 1991 1991 CD cord-328082-7c7slfbp 335 29 ) ) -RRB- cord-328082-7c7slfbp 335 30 were be VBD cord-328082-7c7slfbp 335 31 absent absent JJ cord-328082-7c7slfbp 335 32 from from IN cord-328082-7c7slfbp 335 33 the the DT cord-328082-7c7slfbp 335 34 Golgi Golgi NNP cord-328082-7c7slfbp 335 35 region region NN cord-328082-7c7slfbp 335 36 and and CC cord-328082-7c7slfbp 335 37 p58 p58 NN cord-328082-7c7slfbp 335 38 was be VBD cord-328082-7c7slfbp 335 39 found find VBN cord-328082-7c7slfbp 335 40 only only RB cord-328082-7c7slfbp 335 41 to to TO cord-328082-7c7slfbp 335 42 be be VB cord-328082-7c7slfbp 335 43 localized localize VBN cord-328082-7c7slfbp 335 44 in in IN cord-328082-7c7slfbp 335 45 a a DT cord-328082-7c7slfbp 335 46 few few JJ cord-328082-7c7slfbp 335 47 small small JJ cord-328082-7c7slfbp 335 48 vesicles vesicle NNS cord-328082-7c7slfbp 335 49 associated associate VBN cord-328082-7c7slfbp 335 50 with with IN cord-328082-7c7slfbp 335 51 the the DT cord-328082-7c7slfbp 335 52 clusters cluster NNS cord-328082-7c7slfbp 335 53 of of IN cord-328082-7c7slfbp 335 54 vesicles vesicle NNS cord-328082-7c7slfbp 335 55 in in IN cord-328082-7c7slfbp 335 56 the the DT cord-328082-7c7slfbp 335 57 Golgi Golgi NNP cord-328082-7c7slfbp 335 58 region region NN cord-328082-7c7slfbp 335 59 . . . cord-328082-7c7slfbp 336 1 Even even RB cord-328082-7c7slfbp 336 2 though though IN cord-328082-7c7slfbp 336 3 immunoperoxidase immunoperoxidase NN cord-328082-7c7slfbp 336 4 technique technique NN cord-328082-7c7slfbp 336 5 is be VBZ cord-328082-7c7slfbp 336 6 by by IN cord-328082-7c7slfbp 336 7 no no DT cord-328082-7c7slfbp 336 8 means means NN cord-328082-7c7slfbp 336 9 a a DT cord-328082-7c7slfbp 336 10 quantitative quantitative JJ cord-328082-7c7slfbp 336 11 method method NN cord-328082-7c7slfbp 336 12 , , , cord-328082-7c7slfbp 336 13 when when WRB cord-328082-7c7slfbp 336 14 compared compare VBN cord-328082-7c7slfbp 336 15 with with IN cord-328082-7c7slfbp 336 16 the the DT cord-328082-7c7slfbp 336 17 control control NN cord-328082-7c7slfbp 336 18 cells cell NNS cord-328082-7c7slfbp 336 19 , , , cord-328082-7c7slfbp 336 20 the the DT cord-328082-7c7slfbp 336 21 number number NN cord-328082-7c7slfbp 336 22 of of IN cord-328082-7c7slfbp 336 23 p58-positive p58-positive JJ cord-328082-7c7slfbp 336 24 membrane membrane NN cord-328082-7c7slfbp 336 25 elements element NNS cord-328082-7c7slfbp 336 26 in in IN cord-328082-7c7slfbp 336 27 the the DT cord-328082-7c7slfbp 336 28 Golgi Golgi NNP cord-328082-7c7slfbp 336 29 region region NN cord-328082-7c7slfbp 336 30 seemed seem VBD cord-328082-7c7slfbp 336 31 to to TO cord-328082-7c7slfbp 336 32 be be VB cord-328082-7c7slfbp 336 33 lower low JJR cord-328082-7c7slfbp 336 34 in in IN cord-328082-7c7slfbp 336 35 caffeine caffeine NN cord-328082-7c7slfbp 336 36 - - HYPH cord-328082-7c7slfbp 336 37 treated treat VBN cord-328082-7c7slfbp 336 38 cells cell NNS cord-328082-7c7slfbp 336 39 at at IN cord-328082-7c7slfbp 336 40 reduced reduce VBN cord-328082-7c7slfbp 336 41 temperature temperature NN cord-328082-7c7slfbp 336 42 . . . cord-328082-7c7slfbp 337 1 The the DT cord-328082-7c7slfbp 337 2 statement statement NN cord-328082-7c7slfbp 337 3 that that WDT cord-328082-7c7slfbp 337 4 p58 p58 NNP cord-328082-7c7slfbp 337 5 is be VBZ cord-328082-7c7slfbp 337 6 translocated translocate VBN cord-328082-7c7slfbp 337 7 away away RB cord-328082-7c7slfbp 337 8 from from IN cord-328082-7c7slfbp 337 9 the the DT cord-328082-7c7slfbp 337 10 perinuclear perinuclear JJ cord-328082-7c7slfbp 337 11 1o 1o CD cord-328082-7c7slfbp 337 12 - - HYPH cord-328082-7c7slfbp 337 13 calization calization NN cord-328082-7c7slfbp 337 14 in in IN cord-328082-7c7slfbp 337 15 caffeine caffeine NN cord-328082-7c7slfbp 337 16 - - HYPH cord-328082-7c7slfbp 337 17 treated treat VBN cord-328082-7c7slfbp 337 18 cells cell NNS cord-328082-7c7slfbp 337 19 is be VBZ cord-328082-7c7slfbp 337 20 based base VBN cord-328082-7c7slfbp 337 21 on on IN cord-328082-7c7slfbp 337 22 the the DT cord-328082-7c7slfbp 337 23 results result NNS cord-328082-7c7slfbp 337 24 obtained obtain VBN cord-328082-7c7slfbp 337 25 in in IN cord-328082-7c7slfbp 337 26 the the DT cord-328082-7c7slfbp 337 27 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 337 28 experiments experiment NNS cord-328082-7c7slfbp 337 29 , , , cord-328082-7c7slfbp 337 30 where where WRB cord-328082-7c7slfbp 337 31 distribution distribution NN cord-328082-7c7slfbp 337 32 of of IN cord-328082-7c7slfbp 337 33 the the DT cord-328082-7c7slfbp 337 34 bulk bulk NN cord-328082-7c7slfbp 337 35 of of IN cord-328082-7c7slfbp 337 36 p58 p58 NNP cord-328082-7c7slfbp 337 37 can can MD cord-328082-7c7slfbp 337 38 be be VB cord-328082-7c7slfbp 337 39 observed observe VBN cord-328082-7c7slfbp 337 40 . . . cord-328082-7c7slfbp 338 1 Overall overall RB cord-328082-7c7slfbp 338 2 , , , cord-328082-7c7slfbp 338 3 the the DT cord-328082-7c7slfbp 338 4 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 338 5 microscopic microscopic JJ cord-328082-7c7slfbp 338 6 finding finding NN cord-328082-7c7slfbp 338 7 is be VBZ cord-328082-7c7slfbp 338 8 in in IN cord-328082-7c7slfbp 338 9 agreement agreement NN cord-328082-7c7slfbp 338 10 with with IN cord-328082-7c7slfbp 338 11 the the DT cord-328082-7c7slfbp 338 12 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 338 13 result result NN cord-328082-7c7slfbp 338 14 in in IN cord-328082-7c7slfbp 338 15 Fig Fig NNP cord-328082-7c7slfbp 338 16 . . . cord-328082-7c7slfbp 339 1 5 5 CD cord-328082-7c7slfbp 339 2 a. a. NN cord-328082-7c7slfbp 340 1 As as IN cord-328082-7c7slfbp 340 2 suggested suggest VBN cord-328082-7c7slfbp 340 3 by by IN cord-328082-7c7slfbp 340 4 the the DT cord-328082-7c7slfbp 340 5 immunofluorescence immunofluorescence NN cord-328082-7c7slfbp 340 6 experiments experiment NNS cord-328082-7c7slfbp 340 7 , , , cord-328082-7c7slfbp 340 8 the the DT cord-328082-7c7slfbp 340 9 bulk bulk NN cord-328082-7c7slfbp 340 10 of of IN cord-328082-7c7slfbp 340 11 the the DT cord-328082-7c7slfbp 340 12 p58 p58 NNP cord-328082-7c7slfbp 340 13 seems seem VBZ cord-328082-7c7slfbp 340 14 to to TO cord-328082-7c7slfbp 340 15 be be VB cord-328082-7c7slfbp 340 16 translocated translocate VBN cord-328082-7c7slfbp 340 17 away away RB cord-328082-7c7slfbp 340 18 from from IN cord-328082-7c7slfbp 340 19 the the DT cord-328082-7c7slfbp 340 20 perinuclear perinuclear NNP cord-328082-7c7slfbp 340 21 localization localization NN cord-328082-7c7slfbp 340 22 in in IN cord-328082-7c7slfbp 340 23 caffeine caffeine NN cord-328082-7c7slfbp 340 24 - - HYPH cord-328082-7c7slfbp 340 25 treated treat VBN cord-328082-7c7slfbp 340 26 cells cell NNS cord-328082-7c7slfbp 340 27 . . . cord-328082-7c7slfbp 341 1 These these DT cord-328082-7c7slfbp 341 2 resuits resuit NNS cord-328082-7c7slfbp 341 3 suggest suggest VBP cord-328082-7c7slfbp 341 4 that that IN cord-328082-7c7slfbp 341 5 caffeine caffeine NN cord-328082-7c7slfbp 341 6 and and CC cord-328082-7c7slfbp 341 7 reduced reduce VBN cord-328082-7c7slfbp 341 8 temperature temperature NN cord-328082-7c7slfbp 341 9 are be VBP cord-328082-7c7slfbp 341 10 able able JJ cord-328082-7c7slfbp 341 11 to to TO cord-328082-7c7slfbp 341 12 induce induce VB cord-328082-7c7slfbp 341 13 a a DT cord-328082-7c7slfbp 341 14 selective selective JJ cord-328082-7c7slfbp 341 15 retrograde retrograde JJ cord-328082-7c7slfbp 341 16 translocation translocation NN cord-328082-7c7slfbp 341 17 of of IN cord-328082-7c7slfbp 341 18 pre pre JJ cord-328082-7c7slfbp 341 19 - - JJ cord-328082-7c7slfbp 341 20 and and CC cord-328082-7c7slfbp 341 21 cis cis NNP cord-328082-7c7slfbp 341 22 - - HYPH cord-328082-7c7slfbp 341 23 Golgi Golgi NNP cord-328082-7c7slfbp 341 24 membranes membrane NNS cord-328082-7c7slfbp 341 25 while while IN cord-328082-7c7slfbp 341 26 the the DT cord-328082-7c7slfbp 341 27 localization localization NN cord-328082-7c7slfbp 341 28 of of IN cord-328082-7c7slfbp 341 29 the the DT cord-328082-7c7slfbp 341 30 Golgi Golgi NNP cord-328082-7c7slfbp 341 31 stack stack NN cord-328082-7c7slfbp 341 32 membranes membrane NNS cord-328082-7c7slfbp 341 33 remains remain VBZ cord-328082-7c7slfbp 341 34 unaffected unaffected JJ cord-328082-7c7slfbp 341 35 . . . cord-328082-7c7slfbp 342 1 This this DT cord-328082-7c7slfbp 342 2 was be VBD cord-328082-7c7slfbp 342 3 further further RB cord-328082-7c7slfbp 342 4 confirmed confirm VBN cord-328082-7c7slfbp 342 5 by by IN cord-328082-7c7slfbp 342 6 the the DT cord-328082-7c7slfbp 342 7 unaffected unaffected JJ cord-328082-7c7slfbp 342 8 localization localization NN cord-328082-7c7slfbp 342 9 of/~-COP of/~-cop CD cord-328082-7c7slfbp 342 10 protein protein NN cord-328082-7c7slfbp 342 11 . . . cord-328082-7c7slfbp 343 1 Possible possible JJ cord-328082-7c7slfbp 343 2 explanation explanation NN cord-328082-7c7slfbp 343 3 to to IN cord-328082-7c7slfbp 343 4 the the DT cord-328082-7c7slfbp 343 5 observed observed JJ cord-328082-7c7slfbp 343 6 loss loss NN cord-328082-7c7slfbp 343 7 of of IN cord-328082-7c7slfbp 343 8 the the DT cord-328082-7c7slfbp 343 9 perinuclear perinuclear NNP cord-328082-7c7slfbp 343 10 labeling labeling NN cord-328082-7c7slfbp 343 11 of of IN cord-328082-7c7slfbp 343 12 p58 p58 NNP cord-328082-7c7slfbp 343 13 could could MD cord-328082-7c7slfbp 343 14 be be VB cord-328082-7c7slfbp 343 15 that that IN cord-328082-7c7slfbp 343 16 in in IN cord-328082-7c7slfbp 343 17 the the DT cord-328082-7c7slfbp 343 18 presence presence NN cord-328082-7c7slfbp 343 19 of of IN cord-328082-7c7slfbp 343 20 caffeine caffeine NN cord-328082-7c7slfbp 343 21 the the DT cord-328082-7c7slfbp 343 22 pre pre JJ cord-328082-7c7slfbp 343 23 - - JJ cord-328082-7c7slfbp 343 24 and and CC cord-328082-7c7slfbp 343 25 cis cis NNP cord-328082-7c7slfbp 343 26 - - HYPH cord-328082-7c7slfbp 343 27 Golgi Golgi NNP cord-328082-7c7slfbp 343 28 elements element NNS cord-328082-7c7slfbp 343 29 are be VBP cord-328082-7c7slfbp 343 30 induced induce VBN cord-328082-7c7slfbp 343 31 to to TO cord-328082-7c7slfbp 343 32 move move VB cord-328082-7c7slfbp 343 33 toward toward IN cord-328082-7c7slfbp 343 34 the the DT cord-328082-7c7slfbp 343 35 periphery periphery NN cord-328082-7c7slfbp 343 36 of of IN cord-328082-7c7slfbp 343 37 the the DT cord-328082-7c7slfbp 343 38 cell cell NN cord-328082-7c7slfbp 343 39 . . . cord-328082-7c7slfbp 344 1 Another another DT cord-328082-7c7slfbp 344 2 possibility possibility NN cord-328082-7c7slfbp 344 3 is be VBZ cord-328082-7c7slfbp 344 4 that that IN cord-328082-7c7slfbp 344 5 as as IN cord-328082-7c7slfbp 344 6 the the DT cord-328082-7c7slfbp 344 7 membrane membrane NN cord-328082-7c7slfbp 344 8 traffic traffic NN cord-328082-7c7slfbp 344 9 from from IN cord-328082-7c7slfbp 344 10 the the DT cord-328082-7c7slfbp 344 11 ER ER NNP cord-328082-7c7slfbp 344 12 is be VBZ cord-328082-7c7slfbp 344 13 inhibited inhibit VBN cord-328082-7c7slfbp 344 14 and and CC cord-328082-7c7slfbp 344 15 at at IN cord-328082-7c7slfbp 344 16 least least JJS cord-328082-7c7slfbp 344 17 the the DT cord-328082-7c7slfbp 344 18 BFA BFA NNP cord-328082-7c7slfbp 344 19 - - HYPH cord-328082-7c7slfbp 344 20 induced induce VBN cord-328082-7c7slfbp 344 21 retrograde retrograde JJ cord-328082-7c7slfbp 344 22 movement movement NN cord-328082-7c7slfbp 344 23 of of IN cord-328082-7c7slfbp 344 24 membranes membrane NNS cord-328082-7c7slfbp 344 25 is be VBZ cord-328082-7c7slfbp 344 26 not not RB cord-328082-7c7slfbp 344 27 inhibited inhibit VBN cord-328082-7c7slfbp 344 28 , , , cord-328082-7c7slfbp 344 29 p58 p58 NNP cord-328082-7c7slfbp 344 30 could could MD cord-328082-7c7slfbp 344 31 finally finally RB cord-328082-7c7slfbp 344 32 be be VB cord-328082-7c7slfbp 344 33 recycled recycle VBN cord-328082-7c7slfbp 344 34 to to IN cord-328082-7c7slfbp 344 35 the the DT cord-328082-7c7slfbp 344 36 ER ER NNP cord-328082-7c7slfbp 344 37 , , , cord-328082-7c7slfbp 344 38 being be VBG cord-328082-7c7slfbp 344 39 unable unable JJ cord-328082-7c7slfbp 344 40 to to TO cord-328082-7c7slfbp 344 41 exit exit VB cord-328082-7c7slfbp 344 42 from from IN cord-328082-7c7slfbp 344 43 there there RB cord-328082-7c7slfbp 344 44 . . . cord-328082-7c7slfbp 345 1 Whatever whatever WDT cord-328082-7c7slfbp 345 2 the the DT cord-328082-7c7slfbp 345 3 solution solution NN cord-328082-7c7slfbp 345 4 turns turn VBZ cord-328082-7c7slfbp 345 5 out out RP cord-328082-7c7slfbp 345 6 to to TO cord-328082-7c7slfbp 345 7 be be VB cord-328082-7c7slfbp 345 8 , , , cord-328082-7c7slfbp 345 9 caffeine caffeine NN cord-328082-7c7slfbp 345 10 seems seem VBZ cord-328082-7c7slfbp 345 11 to to TO cord-328082-7c7slfbp 345 12 make make VB cord-328082-7c7slfbp 345 13 it -PRON- PRP cord-328082-7c7slfbp 345 14 possible possible JJ cord-328082-7c7slfbp 345 15 to to TO cord-328082-7c7slfbp 345 16 manipulate manipulate VB cord-328082-7c7slfbp 345 17 the the DT cord-328082-7c7slfbp 345 18 membranes membrane NNS cord-328082-7c7slfbp 345 19 operating operate VBG cord-328082-7c7slfbp 345 20 in in IN cord-328082-7c7slfbp 345 21 the the DT cord-328082-7c7slfbp 345 22 ER er NN cord-328082-7c7slfbp 345 23 - - HYPH cord-328082-7c7slfbp 345 24 to to IN cord-328082-7c7slfbp 345 25 - - HYPH cord-328082-7c7slfbp 345 26 Golgi golgi NN cord-328082-7c7slfbp 345 27 traffic traffic NN cord-328082-7c7slfbp 345 28 in in IN cord-328082-7c7slfbp 345 29 a a DT cord-328082-7c7slfbp 345 30 novel novel JJ cord-328082-7c7slfbp 345 31 way way NN cord-328082-7c7slfbp 345 32 . . . cord-328082-7c7slfbp 346 1 Interestingly interestingly RB cord-328082-7c7slfbp 346 2 , , , cord-328082-7c7slfbp 346 3 Lewis Lewis NNP cord-328082-7c7slfbp 346 4 and and CC cord-328082-7c7slfbp 346 5 Pelham Pelham NNP cord-328082-7c7slfbp 346 6 ( ( -LRB- cord-328082-7c7slfbp 346 7 1992 1992 CD cord-328082-7c7slfbp 346 8 ) ) -RRB- cord-328082-7c7slfbp 346 9 have have VBP cord-328082-7c7slfbp 346 10 recently recently RB cord-328082-7c7slfbp 346 11 shown show VBN cord-328082-7c7slfbp 346 12 that that IN cord-328082-7c7slfbp 346 13 , , , cord-328082-7c7slfbp 346 14 by by IN cord-328082-7c7slfbp 346 15 overexpressing overexpresse VBG cord-328082-7c7slfbp 346 16 the the DT cord-328082-7c7slfbp 346 17 ligand ligand NN cord-328082-7c7slfbp 346 18 for for IN cord-328082-7c7slfbp 346 19 the the DT cord-328082-7c7slfbp 346 20 KDEL KDEL NNP cord-328082-7c7slfbp 346 21 receptor receptor NN cord-328082-7c7slfbp 346 22 , , , cord-328082-7c7slfbp 346 23 the the DT cord-328082-7c7slfbp 346 24 bulk bulk NN cord-328082-7c7slfbp 346 25 of of IN cord-328082-7c7slfbp 346 26 the the DT cord-328082-7c7slfbp 346 27 receptor receptor NN cord-328082-7c7slfbp 346 28 is be VBZ cord-328082-7c7slfbp 346 29 translocated translocate VBN cord-328082-7c7slfbp 346 30 to to IN cord-328082-7c7slfbp 346 31 the the DT cord-328082-7c7slfbp 346 32 ER ER NNP cord-328082-7c7slfbp 346 33 . . . cord-328082-7c7slfbp 347 1 To to TO cord-328082-7c7slfbp 347 2 obtain obtain VB cord-328082-7c7slfbp 347 3 a a DT cord-328082-7c7slfbp 347 4 more more RBR cord-328082-7c7slfbp 347 5 detailed detailed JJ cord-328082-7c7slfbp 347 6 picture picture NN cord-328082-7c7slfbp 347 7 of of IN cord-328082-7c7slfbp 347 8 the the DT cord-328082-7c7slfbp 347 9 effect effect NN cord-328082-7c7slfbp 347 10 of of IN cord-328082-7c7slfbp 347 11 caffeine caffeine NN cord-328082-7c7slfbp 347 12 and and CC cord-328082-7c7slfbp 347 13 reduced reduce VBN cord-328082-7c7slfbp 347 14 temperature temperature NN cord-328082-7c7slfbp 347 15 on on IN cord-328082-7c7slfbp 347 16 the the DT cord-328082-7c7slfbp 347 17 membranes membrane NNS cord-328082-7c7slfbp 347 18 operating operate VBG cord-328082-7c7slfbp 347 19 between between IN cord-328082-7c7slfbp 347 20 the the DT cord-328082-7c7slfbp 347 21 ER ER NNP cord-328082-7c7slfbp 347 22 and and CC cord-328082-7c7slfbp 347 23 the the DT cord-328082-7c7slfbp 347 24 Golgi Golgi NNP cord-328082-7c7slfbp 347 25 complex complex NN cord-328082-7c7slfbp 347 26 , , , cord-328082-7c7slfbp 347 27 it -PRON- PRP cord-328082-7c7slfbp 347 28 is be VBZ cord-328082-7c7slfbp 347 29 necessary necessary JJ cord-328082-7c7slfbp 347 30 to to TO cord-328082-7c7slfbp 347 31 study study VB cord-328082-7c7slfbp 347 32 whether whether IN cord-328082-7c7slfbp 347 33 the the DT cord-328082-7c7slfbp 347 34 distribution distribution NN cord-328082-7c7slfbp 347 35 of of IN cord-328082-7c7slfbp 347 36 other other JJ cord-328082-7c7slfbp 347 37 markers marker NNS cord-328082-7c7slfbp 347 38 for for IN cord-328082-7c7slfbp 347 39 these these DT cord-328082-7c7slfbp 347 40 membranes membrane NNS cord-328082-7c7slfbp 347 41 such such JJ cord-328082-7c7slfbp 347 42 as as IN cord-328082-7c7slfbp 347 43 p53 p53 NN cord-328082-7c7slfbp 347 44 ( ( -LRB- cord-328082-7c7slfbp 347 45 Schweizer Schweizer NNP cord-328082-7c7slfbp 347 46 et et NNP cord-328082-7c7slfbp 347 47 al al NNP cord-328082-7c7slfbp 347 48 . . NNP cord-328082-7c7slfbp 347 49 , , , cord-328082-7c7slfbp 347 50 1988 1988 CD cord-328082-7c7slfbp 347 51 ) ) -RRB- cord-328082-7c7slfbp 347 52 , , , cord-328082-7c7slfbp 347 53 Corona Corona NNP cord-328082-7c7slfbp 347 54 virus virus NN cord-328082-7c7slfbp 347 55 E1 e1 NN cord-328082-7c7slfbp 347 56 glycoprotein glycoprotein NN cord-328082-7c7slfbp 347 57 ( ( -LRB- cord-328082-7c7slfbp 347 58 Machamer Machamer NNP cord-328082-7c7slfbp 347 59 et et NNP cord-328082-7c7slfbp 347 60 al al NNP cord-328082-7c7slfbp 347 61 . . NNP cord-328082-7c7slfbp 347 62 , , , cord-328082-7c7slfbp 347 63 1990 1990 CD cord-328082-7c7slfbp 347 64 ) ) -RRB- cord-328082-7c7slfbp 347 65 , , , cord-328082-7c7slfbp 347 66 and and CC cord-328082-7c7slfbp 347 67 rab2 rab2 NN cord-328082-7c7slfbp 347 68 ( ( -LRB- cord-328082-7c7slfbp 347 69 Chavrier Chavrier NNP cord-328082-7c7slfbp 347 70 et et NNP cord-328082-7c7slfbp 347 71 al al NNP cord-328082-7c7slfbp 347 72 . . NNP cord-328082-7c7slfbp 347 73 , , , cord-328082-7c7slfbp 347 74 1990 1990 CD cord-328082-7c7slfbp 347 75 ) ) -RRB- cord-328082-7c7slfbp 347 76 , , , cord-328082-7c7slfbp 347 77 is be VBZ cord-328082-7c7slfbp 347 78 also also RB cord-328082-7c7slfbp 347 79 affected affect VBN cord-328082-7c7slfbp 347 80 . . . cord-328082-7c7slfbp 348 1 Caffeine caffeine NN cord-328082-7c7slfbp 348 2 at at IN cord-328082-7c7slfbp 348 3 reduced reduce VBN cord-328082-7c7slfbp 348 4 temperature temperature NN cord-328082-7c7slfbp 348 5 on on IN cord-328082-7c7slfbp 348 6 the the DT cord-328082-7c7slfbp 348 7 one one CD cord-328082-7c7slfbp 348 8 hand hand NN cord-328082-7c7slfbp 348 9 inhibited inhibit VBD cord-328082-7c7slfbp 348 10 the the DT cord-328082-7c7slfbp 348 11 exit exit NN cord-328082-7c7slfbp 348 12 of of IN cord-328082-7c7slfbp 348 13 proteins protein NNS cord-328082-7c7slfbp 348 14 from from IN cord-328082-7c7slfbp 348 15 the the DT cord-328082-7c7slfbp 348 16 ER ER NNP cord-328082-7c7slfbp 348 17 and and CC cord-328082-7c7slfbp 348 18 on on IN cord-328082-7c7slfbp 348 19 the the DT cord-328082-7c7slfbp 348 20 other other JJ cord-328082-7c7slfbp 348 21 hand hand NN cord-328082-7c7slfbp 348 22 induced induce VBD cord-328082-7c7slfbp 348 23 a a DT cord-328082-7c7slfbp 348 24 selective selective JJ cord-328082-7c7slfbp 348 25 translocation translocation NN cord-328082-7c7slfbp 348 26 of of IN cord-328082-7c7slfbp 348 27 pre pre JJ cord-328082-7c7slfbp 348 28 - - JJ cord-328082-7c7slfbp 348 29 and and CC cord-328082-7c7slfbp 348 30 cis cis NNP cord-328082-7c7slfbp 348 31 - - HYPH cord-328082-7c7slfbp 348 32 Golgi Golgi NNP cord-328082-7c7slfbp 348 33 membranes membrane NNS cord-328082-7c7slfbp 348 34 to to IN cord-328082-7c7slfbp 348 35 peripheral peripheral JJ cord-328082-7c7slfbp 348 36 locations location NNS cord-328082-7c7slfbp 348 37 ; ; : cord-328082-7c7slfbp 348 38 this this DT cord-328082-7c7slfbp 348 39 is be VBZ cord-328082-7c7slfbp 348 40 in in IN cord-328082-7c7slfbp 348 41 contrast contrast NN cord-328082-7c7slfbp 348 42 to to IN cord-328082-7c7slfbp 348 43 the the DT cord-328082-7c7slfbp 348 44 BFA BFA NNP cord-328082-7c7slfbp 348 45 action action NN cord-328082-7c7slfbp 348 46 which which WDT cord-328082-7c7slfbp 348 47 seems seem VBZ cord-328082-7c7slfbp 348 48 to to TO cord-328082-7c7slfbp 348 49 translocate translocate VB cord-328082-7c7slfbp 348 50 unselectively unselectively RB cord-328082-7c7slfbp 348 51 all all DT cord-328082-7c7slfbp 348 52 Golgi Golgi NNP cord-328082-7c7slfbp 348 53 membranes membrane NNS cord-328082-7c7slfbp 348 54 from from IN cord-328082-7c7slfbp 348 55 their -PRON- PRP$ cord-328082-7c7slfbp 348 56 normal normal JJ cord-328082-7c7slfbp 348 57 perinuclear perinuclear NN cord-328082-7c7slfbp 348 58 location location NN cord-328082-7c7slfbp 349 1 ( ( -LRB- cord-328082-7c7slfbp 349 2 Lippincott Lippincott NNP cord-328082-7c7slfbp 349 3 - - HYPH cord-328082-7c7slfbp 349 4 Schwartz Schwartz NNP cord-328082-7c7slfbp 349 5 et et NNP cord-328082-7c7slfbp 349 6 al al NNP cord-328082-7c7slfbp 349 7 . . NNP cord-328082-7c7slfbp 349 8 , , , cord-328082-7c7slfbp 349 9 1989 1989 CD cord-328082-7c7slfbp 349 10 , , , cord-328082-7c7slfbp 350 1 1991a 1991a CD cord-328082-7c7slfbp 350 2 Doms Doms NNPS cord-328082-7c7slfbp 350 3 et et NNP cord-328082-7c7slfbp 350 4 al al NNP cord-328082-7c7slfbp 350 5 . . . cord-328082-7c7slfbp 351 1 , , , cord-328082-7c7slfbp 351 2 1989 1989 CD cord-328082-7c7slfbp 351 3 ; ; : cord-328082-7c7slfbp 351 4 Fujiwara Fujiwara NNP cord-328082-7c7slfbp 351 5 et et NNP cord-328082-7c7slfbp 351 6 al al NNP cord-328082-7c7slfbp 351 7 . . NNP cord-328082-7c7slfbp 351 8 , , , cord-328082-7c7slfbp 351 9 1988 1988 CD cord-328082-7c7slfbp 351 10 ; ; : cord-328082-7c7slfbp 351 11 Strous Strous NNP cord-328082-7c7slfbp 351 12 et et NNP cord-328082-7c7slfbp 351 13 al al NNP cord-328082-7c7slfbp 351 14 . . NNP cord-328082-7c7slfbp 351 15 , , , cord-328082-7c7slfbp 351 16 1991 1991 CD cord-328082-7c7slfbp 351 17 ) ) -RRB- cord-328082-7c7slfbp 351 18 . . . cord-328082-7c7slfbp 352 1 Further further RB cord-328082-7c7slfbp 352 2 , , , cord-328082-7c7slfbp 352 3 caffeine caffeine NN cord-328082-7c7slfbp 352 4 did do VBD cord-328082-7c7slfbp 352 5 not not RB cord-328082-7c7slfbp 352 6 detach detach VB cord-328082-7c7slfbp 352 7 H h NN cord-328082-7c7slfbp 352 8 - - HYPH cord-328082-7c7slfbp 352 9 COP cop NN cord-328082-7c7slfbp 352 10 from from IN cord-328082-7c7slfbp 352 11 the the DT cord-328082-7c7slfbp 352 12 Golgi Golgi NNP cord-328082-7c7slfbp 352 13 membranes membrane NNS cord-328082-7c7slfbp 352 14 nor nor CC cord-328082-7c7slfbp 352 15 did do VBD cord-328082-7c7slfbp 352 16 it -PRON- PRP cord-328082-7c7slfbp 352 17 translocate translocate VB cord-328082-7c7slfbp 352 18 man man NN cord-328082-7c7slfbp 352 19 II II NNP cord-328082-7c7slfbp 352 20 to to IN cord-328082-7c7slfbp 352 21 the the DT cord-328082-7c7slfbp 352 22 ER er NN cord-328082-7c7slfbp 352 23 . . . cord-328082-7c7slfbp 353 1 Thus thus RB cord-328082-7c7slfbp 353 2 , , , cord-328082-7c7slfbp 353 3 the the DT cord-328082-7c7slfbp 353 4 effect effect NN cord-328082-7c7slfbp 353 5 of of IN cord-328082-7c7slfbp 353 6 caffeine caffeine NN cord-328082-7c7slfbp 353 7 on on IN cord-328082-7c7slfbp 353 8 the the DT cord-328082-7c7slfbp 353 9 organization organization NN cord-328082-7c7slfbp 353 10 of of IN cord-328082-7c7slfbp 353 11 the the DT cord-328082-7c7slfbp 353 12 membranes membrane NNS cord-328082-7c7slfbp 353 13 between between IN cord-328082-7c7slfbp 353 14 the the DT cord-328082-7c7slfbp 353 15 ER ER NNP cord-328082-7c7slfbp 353 16 and and CC cord-328082-7c7slfbp 353 17 the the DT cord-328082-7c7slfbp 353 18 Golgi Golgi NNP cord-328082-7c7slfbp 353 19 complex complex NN cord-328082-7c7slfbp 353 20 is be VBZ cord-328082-7c7slfbp 353 21 clearly clearly RB cord-328082-7c7slfbp 353 22 different different JJ cord-328082-7c7slfbp 353 23 from from IN cord-328082-7c7slfbp 353 24 that that DT cord-328082-7c7slfbp 353 25 induced induce VBN cord-328082-7c7slfbp 353 26 by by IN cord-328082-7c7slfbp 353 27 BFA BFA NNP cord-328082-7c7slfbp 353 28 . . . cord-328082-7c7slfbp 354 1 The the DT cord-328082-7c7slfbp 354 2 different different JJ cord-328082-7c7slfbp 354 3 effect effect NN cord-328082-7c7slfbp 354 4 of of IN cord-328082-7c7slfbp 354 5 BFA bfa NN cord-328082-7c7slfbp 354 6 and and CC cord-328082-7c7slfbp 354 7 caffeine caffeine NN cord-328082-7c7slfbp 354 8 on on IN cord-328082-7c7slfbp 354 9 the the DT cord-328082-7c7slfbp 354 10 Golgi Golgi NNP cord-328082-7c7slfbp 354 11 complex complex NN cord-328082-7c7slfbp 354 12 would would MD cord-328082-7c7slfbp 354 13 indicate indicate VB cord-328082-7c7slfbp 354 14 that that IN cord-328082-7c7slfbp 354 15 the the DT cord-328082-7c7slfbp 354 16 Golgi Golgi NNP cord-328082-7c7slfbp 354 17 complex complex NN cord-328082-7c7slfbp 354 18 is be VBZ cord-328082-7c7slfbp 354 19 composed compose VBN cord-328082-7c7slfbp 354 20 of of IN cord-328082-7c7slfbp 354 21 two two CD cord-328082-7c7slfbp 354 22 subsets subset NNS cord-328082-7c7slfbp 354 23 of of IN cord-328082-7c7slfbp 354 24 homotypic homotypic JJ cord-328082-7c7slfbp 354 25 membranes membrane NNS cord-328082-7c7slfbp 354 26 differentially differentially RB cord-328082-7c7slfbp 354 27 sensitive sensitive JJ cord-328082-7c7slfbp 354 28 to to IN cord-328082-7c7slfbp 354 29 caffeine caffeine NN cord-328082-7c7slfbp 354 30 treatment treatment NN cord-328082-7c7slfbp 354 31 . . . cord-328082-7c7slfbp 355 1 Whether whether IN cord-328082-7c7slfbp 355 2 this this DT cord-328082-7c7slfbp 355 3 is be VBZ cord-328082-7c7slfbp 355 4 also also RB cord-328082-7c7slfbp 355 5 reflected reflect VBN cord-328082-7c7slfbp 355 6 in in IN cord-328082-7c7slfbp 355 7 the the DT cord-328082-7c7slfbp 355 8 differences difference NNS cord-328082-7c7slfbp 355 9 of of IN cord-328082-7c7slfbp 355 10 the the DT cord-328082-7c7slfbp 355 11 coat coat NN cord-328082-7c7slfbp 355 12 structures structure NNS cord-328082-7c7slfbp 355 13 of of IN cord-328082-7c7slfbp 355 14 these these DT cord-328082-7c7slfbp 355 15 membranes membrane NNS cord-328082-7c7slfbp 355 16 remains remain VBZ cord-328082-7c7slfbp 355 17 to to TO cord-328082-7c7slfbp 355 18 be be VB cord-328082-7c7slfbp 355 19 seen see VBN cord-328082-7c7slfbp 355 20 . . . cord-328082-7c7slfbp 356 1 From from IN cord-328082-7c7slfbp 356 2 G g NN cord-328082-7c7slfbp 356 3 minor minor NN cord-328082-7c7slfbp 356 4 to to IN cord-328082-7c7slfbp 356 5 G g NN cord-328082-7c7slfbp 356 6 major major JJ cord-328082-7c7slfbp 356 7 Reconstitution reconstitution NN cord-328082-7c7slfbp 356 8 of of IN cord-328082-7c7slfbp 356 9 the the DT cord-328082-7c7slfbp 356 10 transport transport NN cord-328082-7c7slfbp 356 11 of of IN cord-328082-7c7slfbp 356 12 protein protein NN cord-328082-7c7slfbp 356 13 between between IN cord-328082-7c7slfbp 356 14 successive successive JJ cord-328082-7c7slfbp 356 15 compartments compartment NNS cord-328082-7c7slfbp 356 16 of of IN cord-328082-7c7slfbp 356 17 the the DT cord-328082-7c7slfbp 356 18 Golgi Golgi NNP cord-328082-7c7slfbp 356 19 measured measure VBN cord-328082-7c7slfbp 356 20 by by IN cord-328082-7c7slfbp 356 21 the the DT cord-328082-7c7slfbp 356 22 coupled coupled JJ cord-328082-7c7slfbp 356 23 incorporation incorporation NN cord-328082-7c7slfbp 356 24 of of IN cord-328082-7c7slfbp 356 25 N N NNP cord-328082-7c7slfbp 356 26 - - HYPH cord-328082-7c7slfbp 356 27 acetylglucosamine acetylglucosamine NNP cord-328082-7c7slfbp 356 28 ATP ATP NNP cord-328082-7c7slfbp 356 29 - - HYPH cord-328082-7c7slfbp 356 30 coupled couple VBN cord-328082-7c7slfbp 356 31 transport transport NN cord-328082-7c7slfbp 356 32 of of IN cord-328082-7c7slfbp 356 33 Vesicular Vesicular NNP cord-328082-7c7slfbp 356 34 Stomatitis Stomatitis NNP cord-328082-7c7slfbp 356 35 virus virus NN cord-328082-7c7slfbp 356 36 G g NN cord-328082-7c7slfbp 356 37 - - HYPH cord-328082-7c7slfbp 356 38 protein protein NN cord-328082-7c7slfbp 356 39 between between IN cord-328082-7c7slfbp 356 40 the the DT cord-328082-7c7slfbp 356 41 endoplasmic endoplasmic NN cord-328082-7c7slfbp 356 42 reticulum reticulum NN cord-328082-7c7slfbp 356 43 and and CC cord-328082-7c7slfbp 356 44 the the DT cord-328082-7c7slfbp 356 45 Goigi Goigi NNP cord-328082-7c7slfbp 356 46 Mannosidase Mannosidase NNP cord-328082-7c7slfbp 356 47 II II NNP cord-328082-7c7slfbp 356 48 and and CC cord-328082-7c7slfbp 356 49 the the DT cord-328082-7c7slfbp 356 50 135-kD 135-kd CD cord-328082-7c7slfbp 356 51 Golgispecific Golgispecific NNP cord-328082-7c7slfbp 356 52 antigen antigen NN cord-328082-7c7slfbp 356 53 recognized recognize VBN cord-328082-7c7slfbp 356 54 by by IN cord-328082-7c7slfbp 356 55 monoclonal monoclonal JJ cord-328082-7c7slfbp 356 56 antibody antibody NN cord-328082-7c7slfbp 357 1 53FC3 53fc3 CD cord-328082-7c7slfbp 357 2 are be VBP cord-328082-7c7slfbp 357 3 the the DT cord-328082-7c7slfbp 357 4 same same JJ cord-328082-7c7slfbp 357 5 dimeric dimeric JJ cord-328082-7c7slfbp 357 6 protein protein NN cord-328082-7c7slfbp 357 7 Trimeric Trimeric NNP cord-328082-7c7slfbp 357 8 G G NNP cord-328082-7c7slfbp 357 9 - - HYPH cord-328082-7c7slfbp 357 10 proteins protein NNS cord-328082-7c7slfbp 357 11 of of IN cord-328082-7c7slfbp 357 12 the the DT cord-328082-7c7slfbp 357 13 trans trans NNP cord-328082-7c7slfbp 357 14 - - NNP cord-328082-7c7slfbp 357 15 Golgi Golgi NNP cord-328082-7c7slfbp 357 16 network network NN cord-328082-7c7slfbp 357 17 are be VBP cord-328082-7c7slfbp 357 18 involved involve VBN cord-328082-7c7slfbp 357 19 in in IN cord-328082-7c7slfbp 357 20 the the DT cord-328082-7c7slfbp 357 21 formation formation NN cord-328082-7c7slfbp 357 22 of of IN cord-328082-7c7slfbp 357 23 constitutive constitutive JJ cord-328082-7c7slfbp 357 24 secretory secretory JJ cord-328082-7c7slfbp 357 25 vesicles vesicle NNS cord-328082-7c7slfbp 357 26 and and CC cord-328082-7c7slfbp 357 27 immature immature JJ cord-328082-7c7slfbp 357 28 secretory secretory NN cord-328082-7c7slfbp 357 29 granules granule NNS cord-328082-7c7slfbp 357 30 Trimeric Trimeric NNP cord-328082-7c7slfbp 357 31 G g NN cord-328082-7c7slfbp 357 32 proteins protein NNS cord-328082-7c7slfbp 357 33 and and CC cord-328082-7c7slfbp 357 34 vesicle vesicle NN cord-328082-7c7slfbp 357 35 formation formation NN cord-328082-7c7slfbp 357 36 Calcium calcium NN cord-328082-7c7slfbp 357 37 and and CC cord-328082-7c7slfbp 357 38 GTP GTP NNP cord-328082-7c7slfbp 357 39 : : : cord-328082-7c7slfbp 357 40 essential essential JJ cord-328082-7c7slfbp 357 41 components component NNS cord-328082-7c7slfbp 357 42 in in IN cord-328082-7c7slfbp 357 43 vesicular vesicular JJ cord-328082-7c7slfbp 357 44 trafficking trafficking NN cord-328082-7c7slfbp 357 45 between between IN cord-328082-7c7slfbp 357 46 the the DT cord-328082-7c7slfbp 357 47 endoplasmic endoplasmic NN cord-328082-7c7slfbp 357 48 reticulum reticulum NN cord-328082-7c7slfbp 357 49 and and CC cord-328082-7c7slfbp 357 50 Golgi Golgi NNP cord-328082-7c7slfbp 357 51 apparatus apparatus NN cord-328082-7c7slfbp 357 52 Immunoperoxidase Immunoperoxidase NNP cord-328082-7c7slfbp 357 53 methods method NNS cord-328082-7c7slfbp 357 54 for for IN cord-328082-7c7slfbp 357 55 the the DT cord-328082-7c7slfbp 357 56 localization localization NN cord-328082-7c7slfbp 357 57 of of IN cord-328082-7c7slfbp 357 58 antigens antigen NNS cord-328082-7c7slfbp 357 59 in in IN cord-328082-7c7slfbp 357 60 cultured cultured JJ cord-328082-7c7slfbp 357 61 cells cell NNS cord-328082-7c7slfbp 357 62 and and CC cord-328082-7c7slfbp 357 63 tissue tissue NN cord-328082-7c7slfbp 357 64 sections section NNS cord-328082-7c7slfbp 357 65 by by IN cord-328082-7c7slfbp 357 66 electron electron NN cord-328082-7c7slfbp 357 67 microscopy microscopy NN cord-328082-7c7slfbp 357 68 Distribution distribution NN cord-328082-7c7slfbp 357 69 of of IN cord-328082-7c7slfbp 357 70 two two CD cord-328082-7c7slfbp 357 71 distinct distinct JJ cord-328082-7c7slfbp 357 72 Ca2 ca2 CD cord-328082-7c7slfbp 357 73 + + CC cord-328082-7c7slfbp 358 1 -ATPase -atpase IN cord-328082-7c7slfbp 358 2 like like IN cord-328082-7c7slfbp 358 3 proteins protein NNS cord-328082-7c7slfbp 358 4 and and CC cord-328082-7c7slfbp 358 5 their -PRON- PRP$ cord-328082-7c7slfbp 358 6 relationships relationship NNS cord-328082-7c7slfbp 358 7 to to IN cord-328082-7c7slfbp 358 8 the the DT cord-328082-7c7slfbp 358 9 agonist agonist NN cord-328082-7c7slfbp 358 10 - - HYPH cord-328082-7c7slfbp 358 11 sensitive sensitive JJ cord-328082-7c7slfbp 358 12 calcium calcium NN cord-328082-7c7slfbp 358 13 store store NN cord-328082-7c7slfbp 358 14 in in IN cord-328082-7c7slfbp 358 15 adrenal adrenal JJ cord-328082-7c7slfbp 358 16 chromaffin chromaffin NNP cord-328082-7c7slfbp 358 17 cells cell NNS cord-328082-7c7slfbp 359 1 A a DT cord-328082-7c7slfbp 359 2 monoclonal monoclonal JJ cord-328082-7c7slfbp 359 3 antibody antibody NN cord-328082-7c7slfbp 359 4 against against IN cord-328082-7c7slfbp 359 5 a a DT cord-328082-7c7slfbp 359 6 135 135 CD cord-328082-7c7slfbp 359 7 K K NNP cord-328082-7c7slfbp 359 8 Golgi Golgi NNP cord-328082-7c7slfbp 359 9 membrane membrane NN cord-328082-7c7slfbp 359 10 protein protein NN cord-328082-7c7slfbp 359 11 Adenosin Adenosin NNP cord-328082-7c7slfbp 359 12 Y,5'-phosphate Y,5'-phosphate NNP cord-328082-7c7slfbp 359 13 in in IN cord-328082-7c7slfbp 359 14 biological biological JJ cord-328082-7c7slfbp 359 15 materials material NNS cord-328082-7c7slfbp 359 16 Localization localization NN cord-328082-7c7slfbp 359 17 of of IN cord-328082-7c7slfbp 359 18 lower low JJR cord-328082-7c7slfbp 359 19 molecular molecular JJ cord-328082-7c7slfbp 359 20 weight weight NN cord-328082-7c7slfbp 359 21 GTP gtp NN cord-328082-7c7slfbp 359 22 - - HYPH cord-328082-7c7slfbp 359 23 binding bind VBG cord-328082-7c7slfbp 359 24 proteins protein NNS cord-328082-7c7slfbp 359 25 to to IN cord-328082-7c7slfbp 359 26 exocytic exocytic JJ cord-328082-7c7slfbp 359 27 and and CC cord-328082-7c7slfbp 359 28 endocytic endocytic JJ cord-328082-7c7slfbp 359 29 compartments compartment NNS cord-328082-7c7slfbp 359 30 Evidence evidence NN cord-328082-7c7slfbp 359 31 for for IN cord-328082-7c7slfbp 359 32 the the DT cord-328082-7c7slfbp 359 33 regulation regulation NN cord-328082-7c7slfbp 359 34 of of IN cord-328082-7c7slfbp 359 35 exocytic exocytic JJ cord-328082-7c7slfbp 359 36 transport transport NN cord-328082-7c7slfbp 359 37 by by IN cord-328082-7c7slfbp 359 38 protein protein NN cord-328082-7c7slfbp 359 39 phosphorylation phosphorylation NN cord-328082-7c7slfbp 360 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 360 2 A A NNP cord-328082-7c7slfbp 360 3 redistributes redistribute VBZ cord-328082-7c7slfbp 360 4 resident resident JJ cord-328082-7c7slfbp 360 5 and and CC cord-328082-7c7slfbp 360 6 itinerant itinerant JJ cord-328082-7c7slfbp 360 7 Golgi Golgi NNP cord-328082-7c7slfbp 360 8 proteins protein NNS cord-328082-7c7slfbp 360 9 to to IN cord-328082-7c7slfbp 360 10 the the DT cord-328082-7c7slfbp 360 11 endoplasmic endoplasmic NN cord-328082-7c7slfbp 360 12 reticulum reticulum NN cord-328082-7c7slfbp 360 13 Dissociation Dissociation NNP cord-328082-7c7slfbp 360 14 ofa ofa NN cord-328082-7c7slfbp 360 15 110-kD 110-kd CD cord-328082-7c7slfbp 360 16 peripheral peripheral JJ cord-328082-7c7slfbp 360 17 membrane membrane NN cord-328082-7c7slfbp 360 18 protein protein NN cord-328082-7c7slfbp 360 19 from from IN cord-328082-7c7slfbp 360 20 the the DT cord-328082-7c7slfbp 360 21 Golgi Golgi NNP cord-328082-7c7slfbp 360 22 apparatus apparatus NN cord-328082-7c7slfbp 360 23 is be VBZ cord-328082-7c7slfbp 360 24 an an DT cord-328082-7c7slfbp 360 25 early early JJ cord-328082-7c7slfbp 360 26 event event NN cord-328082-7c7slfbp 360 27 in in IN cord-328082-7c7slfbp 360 28 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 360 29 A A NNP cord-328082-7c7slfbp 360 30 action action NN cord-328082-7c7slfbp 360 31 Caffeine Caffeine NNP cord-328082-7c7slfbp 360 32 overcomes overcome VBZ cord-328082-7c7slfbp 360 33 a a DT cord-328082-7c7slfbp 360 34 restriction restriction NN cord-328082-7c7slfbp 360 35 point point NN cord-328082-7c7slfbp 360 36 associated associate VBN cord-328082-7c7slfbp 360 37 with with IN cord-328082-7c7slfbp 360 38 DNA dna NN cord-328082-7c7slfbp 360 39 replication replication NN cord-328082-7c7slfbp 360 40 , , , cord-328082-7c7slfbp 360 41 but but CC cord-328082-7c7slfbp 360 42 does do VBZ cord-328082-7c7slfbp 360 43 not not RB cord-328082-7c7slfbp 360 44 accelerate accelerate VB cord-328082-7c7slfbp 360 45 mitosis mitosis NN cord-328082-7c7slfbp 360 46 t t NN cord-328082-7c7slfbp 360 47 - - HYPH cord-328082-7c7slfbp 360 48 COP COP NNP cord-328082-7c7slfbp 360 49 , , , cord-328082-7c7slfbp 360 50 a a DT cord-328082-7c7slfbp 360 51 110 110 CD cord-328082-7c7slfbp 360 52 kD kD NNP cord-328082-7c7slfbp 360 53 protein protein NN cord-328082-7c7slfbp 360 54 associated associate VBN cord-328082-7c7slfbp 360 55 with with IN cord-328082-7c7slfbp 360 56 non non JJ cord-328082-7c7slfbp 360 57 - - JJ cord-328082-7c7slfbp 360 58 clathrin clathrin JJ cord-328082-7c7slfbp 360 59 coated coat VBN cord-328082-7c7slfbp 360 60 vesicles vesicle NNS cord-328082-7c7slfbp 360 61 and and CC cord-328082-7c7slfbp 360 62 the the DT cord-328082-7c7slfbp 360 63 Golgi Golgi NNP cord-328082-7c7slfbp 360 64 complex complex NN cord-328082-7c7slfbp 360 65 , , , cord-328082-7c7slfbp 360 66 shows show VBZ cord-328082-7c7slfbp 360 67 homology homology NN cord-328082-7c7slfbp 361 1 to/3-adaptin to/3-adaptin NNP cord-328082-7c7slfbp 361 2 Transport Transport NNP cord-328082-7c7slfbp 361 3 of of IN cord-328082-7c7slfbp 361 4 Vesicular Vesicular NNP cord-328082-7c7slfbp 361 5 Stomatitis Stomatitis NNP cord-328082-7c7slfbp 361 6 virus virus NN cord-328082-7c7slfbp 361 7 glycoprotein glycoprotein NN cord-328082-7c7slfbp 361 8 in in IN cord-328082-7c7slfbp 361 9 a a DT cord-328082-7c7slfbp 361 10 cell cell NN cord-328082-7c7slfbp 361 11 - - HYPH cord-328082-7c7slfbp 361 12 free free JJ cord-328082-7c7slfbp 361 13 extract extract NN cord-328082-7c7slfbp 362 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 362 2 A A NNP cord-328082-7c7slfbp 362 3 causes cause VBZ cord-328082-7c7slfbp 362 4 disassembly disassembly NN cord-328082-7c7slfbp 362 5 of of IN cord-328082-7c7slfbp 362 6 the the DT cord-328082-7c7slfbp 362 7 Oolgi Oolgi NNP cord-328082-7c7slfbp 362 8 complex complex NN cord-328082-7c7slfbp 362 9 and and CC cord-328082-7c7slfbp 362 10 accumulation accumulation NN cord-328082-7c7slfbp 362 11 of of IN cord-328082-7c7slfbp 362 12 secretory secretory JJ cord-328082-7c7slfbp 362 13 proteins protein NNS cord-328082-7c7slfbp 362 14 in in IN cord-328082-7c7slfbp 362 15 the the DT cord-328082-7c7slfbp 362 16 endoplasmic endoplasmic NN cord-328082-7c7slfbp 362 17 reticulum reticulum NN cord-328082-7c7slfbp 362 18 Efficient efficient JJ cord-328082-7c7slfbp 362 19 transport transport NN cord-328082-7c7slfbp 362 20 of of IN cord-328082-7c7slfbp 362 21 Semliki Semliki NNP cord-328082-7c7slfbp 362 22 Forest Forest NNP cord-328082-7c7slfbp 362 23 virus virus NN cord-328082-7c7slfbp 362 24 glycoproteins glycoprotein VBZ cord-328082-7c7slfbp 362 25 through through IN cord-328082-7c7slfbp 362 26 a a DT cord-328082-7c7slfbp 362 27 Golgi Golgi NNP cord-328082-7c7slfbp 362 28 complex complex NN cord-328082-7c7slfbp 362 29 morphologically morphologically RB cord-328082-7c7slfbp 362 30 altered alter VBN cord-328082-7c7slfbp 362 31 by by IN cord-328082-7c7slfbp 362 32 Uukuniemi Uukuniemi NNP cord-328082-7c7slfbp 362 33 virus virus NN cord-328082-7c7slfbp 362 34 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 363 1 Ca ca NN cord-328082-7c7slfbp 363 2 2 2 CD cord-328082-7c7slfbp 363 3 + + CD cord-328082-7c7slfbp 364 1 release release NN cord-328082-7c7slfbp 364 2 from from IN cord-328082-7c7slfbp 364 3 endoplasmic endoplasmic NN cord-328082-7c7slfbp 364 4 reticulum reticulum NN cord-328082-7c7slfbp 364 5 is be VBZ cord-328082-7c7slfbp 364 6 mediated mediate VBN cord-328082-7c7slfbp 364 7 by by IN cord-328082-7c7slfbp 364 8 a a DT cord-328082-7c7slfbp 364 9 guanine guanine NN cord-328082-7c7slfbp 364 10 nucleotide nucleotide NN cord-328082-7c7slfbp 364 11 regulatory regulatory JJ cord-328082-7c7slfbp 364 12 mechanism mechanism NN cord-328082-7c7slfbp 364 13 Die Die NNP cord-328082-7c7slfbp 364 14 konstitutiou konstitutiou NNP cord-328082-7c7slfbp 364 15 von von NNP cord-328082-7c7slfbp 364 16 brefeldin brefeldin NNP cord-328082-7c7slfbp 365 1 A a DT cord-328082-7c7slfbp 365 2 The the DT cord-328082-7c7slfbp 365 3 endoplasmic endoplasmic NN cord-328082-7c7slfbp 365 4 reticulure reticulure NN cord-328082-7c7slfbp 365 5 as as IN cord-328082-7c7slfbp 365 6 a a DT cord-328082-7c7slfbp 365 7 protein protein NN cord-328082-7c7slfbp 365 8 - - HYPH cord-328082-7c7slfbp 365 9 folding fold VBG cord-328082-7c7slfbp 365 10 compartment compartment NN cord-328082-7c7slfbp 365 11 Isolation isolation NN cord-328082-7c7slfbp 365 12 and and CC cord-328082-7c7slfbp 365 13 characterization characterization NN cord-328082-7c7slfbp 365 14 of of IN cord-328082-7c7slfbp 365 15 temperature temperature NN cord-328082-7c7slfbp 365 16 - - HYPH cord-328082-7c7slfbp 365 17 sensitive sensitive JJ cord-328082-7c7slfbp 365 18 mutants mutant NNS cord-328082-7c7slfbp 365 19 from from IN cord-328082-7c7slfbp 365 20 Semliki Semliki NNP cord-328082-7c7slfbp 365 21 Forest Forest NNP cord-328082-7c7slfbp 365 22 Virus Virus NNP cord-328082-7c7slfbp 365 23 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 365 24 A A NNP cord-328082-7c7slfbp 365 25 : : : cord-328082-7c7slfbp 365 26 insights insight NNS cord-328082-7c7slfbp 365 27 into into IN cord-328082-7c7slfbp 365 28 the the DT cord-328082-7c7slfbp 365 29 control control NN cord-328082-7c7slfbp 365 30 of of IN cord-328082-7c7slfbp 365 31 membrane membrane NN cord-328082-7c7slfbp 365 32 traffic traffic NN cord-328082-7c7slfbp 365 33 and and CC cord-328082-7c7slfbp 365 34 organelle organelle NN cord-328082-7c7slfbp 365 35 structure structure NN cord-328082-7c7slfbp 365 36 Role role NN cord-328082-7c7slfbp 365 37 of of IN cord-328082-7c7slfbp 365 38 microtubules microtubule NNS cord-328082-7c7slfbp 365 39 in in IN cord-328082-7c7slfbp 365 40 the the DT cord-328082-7c7slfbp 365 41 organization organization NN cord-328082-7c7slfbp 365 42 of of IN cord-328082-7c7slfbp 365 43 the the DT cord-328082-7c7slfbp 365 44 Golgi Golgi NNP cord-328082-7c7slfbp 365 45 apparatus apparatus NN cord-328082-7c7slfbp 365 46 Low low JJ cord-328082-7c7slfbp 365 47 temperature temperature NN cord-328082-7c7slfbp 365 48 - - HYPH cord-328082-7c7slfbp 365 49 induced induce VBN cord-328082-7c7slfbp 365 50 transport transport NN cord-328082-7c7slfbp 365 51 blocks block NNS cord-328082-7c7slfbp 365 52 as as IN cord-328082-7c7slfbp 365 53 tools tool NNS cord-328082-7c7slfbp 365 54 to to TO cord-328082-7c7slfbp 365 55 manipulate manipulate VB cord-328082-7c7slfbp 365 56 membrane membrane NN cord-328082-7c7slfbp 365 57 traffic traffic NN cord-328082-7c7slfbp 365 58 Effect effect NN cord-328082-7c7slfbp 365 59 of of IN cord-328082-7c7slfbp 365 60 caffeine caffeine NN cord-328082-7c7slfbp 365 61 on on IN cord-328082-7c7slfbp 365 62 intracellular intracellular JJ cord-328082-7c7slfbp 365 63 transport transport NN cord-328082-7c7slfbp 365 64 of of IN cord-328082-7c7slfbp 365 65 Semliki Semliki NNP cord-328082-7c7slfbp 365 66 Forest Forest NNP cord-328082-7c7slfbp 365 67 virus virus NN cord-328082-7c7slfbp 365 68 membrane membrane NN cord-328082-7c7slfbp 365 69 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 365 70 Cleavage cleavage NN cord-328082-7c7slfbp 365 71 of of IN cord-328082-7c7slfbp 365 72 structural structural JJ cord-328082-7c7slfbp 365 73 proteins protein NNS cord-328082-7c7slfbp 365 74 during during IN cord-328082-7c7slfbp 365 75 the the DT cord-328082-7c7slfbp 365 76 assembly assembly NN cord-328082-7c7slfbp 365 77 of of IN cord-328082-7c7slfbp 365 78 the the DT cord-328082-7c7slfbp 365 79 head head NN cord-328082-7c7slfbp 365 80 of of IN cord-328082-7c7slfbp 365 81 bacteriophage bacteriophage NN cord-328082-7c7slfbp 365 82 T4 T4 NNP cord-328082-7c7slfbp 365 83 Characterization Characterization NNP cord-328082-7c7slfbp 365 84 of of IN cord-328082-7c7slfbp 365 85 a a DT cord-328082-7c7slfbp 365 86 58 58 CD cord-328082-7c7slfbp 365 87 kDa kDa NNP cord-328082-7c7slfbp 365 88 cis cis NNP cord-328082-7c7slfbp 365 89 - - HYPH cord-328082-7c7slfbp 365 90 Golgi Golgi NNP cord-328082-7c7slfbp 365 91 protein protein NN cord-328082-7c7slfbp 365 92 in in IN cord-328082-7c7slfbp 365 93 pancreatic pancreatic JJ cord-328082-7c7slfbp 365 94 exocrine exocrine NN cord-328082-7c7slfbp 365 95 cells cell NNS cord-328082-7c7slfbp 365 96 Driving drive VBG cord-328082-7c7slfbp 365 97 the the DT cord-328082-7c7slfbp 365 98 cell cell NN cord-328082-7c7slfbp 365 99 cycle cycle NN cord-328082-7c7slfbp 365 100 : : : cord-328082-7c7slfbp 365 101 M m NN cord-328082-7c7slfbp 365 102 phase phase NN cord-328082-7c7slfbp 365 103 kinase kinase NN cord-328082-7c7slfbp 365 104 , , , cord-328082-7c7slfbp 365 105 its -PRON- PRP$ cord-328082-7c7slfbp 365 106 partners partner NNS cord-328082-7c7slfbp 365 107 , , , cord-328082-7c7slfbp 365 108 and and CC cord-328082-7c7slfbp 365 109 substrates substrate VBZ cord-328082-7c7slfbp 365 110 A a DT cord-328082-7c7slfbp 365 111 human human JJ cord-328082-7c7slfbp 365 112 homologue homologue NN cord-328082-7c7slfbp 365 113 of of IN cord-328082-7c7slfbp 365 114 the the DT cord-328082-7c7slfbp 365 115 yeast yeast NN cord-328082-7c7slfbp 365 116 HDEL HDEL NNP cord-328082-7c7slfbp 365 117 receptor receptor NN cord-328082-7c7slfbp 365 118 Ligand Ligand NNP cord-328082-7c7slfbp 365 119 - - HYPH cord-328082-7c7slfbp 365 120 induee.d induee.d JJ cord-328082-7c7slfbp 365 121 redistribution redistribution NN cord-328082-7c7slfbp 365 122 of of IN cord-328082-7c7slfbp 365 123 a a DT cord-328082-7c7slfbp 365 124 human human JJ cord-328082-7c7slfbp 365 125 KDEL KDEL NNP cord-328082-7c7slfbp 365 126 receptor receptor NN cord-328082-7c7slfbp 365 127 from from IN cord-328082-7c7slfbp 365 128 the the DT cord-328082-7c7slfbp 365 129 Golgi Golgi NNP cord-328082-7c7slfbp 365 130 complex complex NN cord-328082-7c7slfbp 365 131 to to IN cord-328082-7c7slfbp 365 132 the the DT cord-328082-7c7slfbp 365 133 endoplasmic endoplasmic NN cord-328082-7c7slfbp 365 134 reticulure reticulure NN cord-328082-7c7slfbp 366 1 The the DT cord-328082-7c7slfbp 366 2 ERD2 ERD2 NNP cord-328082-7c7slfbp 366 3 gene gene NN cord-328082-7c7slfbp 366 4 determines determine VBZ cord-328082-7c7slfbp 366 5 the the DT cord-328082-7c7slfbp 366 6 specificity specificity NN cord-328082-7c7slfbp 366 7 of of IN cord-328082-7c7slfbp 366 8 the the DT cord-328082-7c7slfbp 366 9 luminal luminal JJ cord-328082-7c7slfbp 366 10 ER ER NNP cord-328082-7c7slfbp 366 11 protein protein NN cord-328082-7c7slfbp 366 12 retention retention NN cord-328082-7c7slfbp 366 13 system system NN cord-328082-7c7slfbp 366 14 Rapid rapid JJ cord-328082-7c7slfbp 366 15 redistribution redistribution NN cord-328082-7c7slfbp 366 16 of of IN cord-328082-7c7slfbp 366 17 Golgi Golgi NNP cord-328082-7c7slfbp 366 18 proteins protein NNS cord-328082-7c7slfbp 366 19 into into IN cord-328082-7c7slfbp 366 20 the the DT cord-328082-7c7slfbp 366 21 ER ER NNP cord-328082-7c7slfbp 366 22 in in IN cord-328082-7c7slfbp 366 23 cells cell NNS cord-328082-7c7slfbp 366 24 treated treat VBN cord-328082-7c7slfbp 366 25 with with IN cord-328082-7c7slfbp 366 26 brefeldin brefeldin NNS cord-328082-7c7slfbp 366 27 A a NN cord-328082-7c7slfbp 366 28 : : : cord-328082-7c7slfbp 366 29 evidence evidence NN cord-328082-7c7slfbp 366 30 of of IN cord-328082-7c7slfbp 366 31 membrane membrane NN cord-328082-7c7slfbp 366 32 cycling cycle VBG cord-328082-7c7slfbp 366 33 from from IN cord-328082-7c7slfbp 366 34 Golgi Golgi NNP cord-328082-7c7slfbp 366 35 to to IN cord-328082-7c7slfbp 366 36 ER ER NNP cord-328082-7c7slfbp 366 37 Microtubule Microtubule NNP cord-328082-7c7slfbp 366 38 - - HYPH cord-328082-7c7slfbp 366 39 dependent dependent JJ cord-328082-7c7slfbp 366 40 retrograde retrograde JJ cord-328082-7c7slfbp 366 41 transport transport NN cord-328082-7c7slfbp 366 42 of of IN cord-328082-7c7slfbp 366 43 proteins protein NNS cord-328082-7c7slfbp 366 44 into into IN cord-328082-7c7slfbp 366 45 the the DT cord-328082-7c7slfbp 366 46 ER ER NNP cord-328082-7c7slfbp 366 47 in in IN cord-328082-7c7slfbp 366 48 the the DT cord-328082-7c7slfbp 366 49 presence presence NN cord-328082-7c7slfbp 366 50 of of IN cord-328082-7c7slfbp 366 51 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 366 52 A A NNP cord-328082-7c7slfbp 366 53 suggest suggest VBP cord-328082-7c7slfbp 366 54 an an DT cord-328082-7c7slfbp 366 55 ER ER NNP cord-328082-7c7slfbp 366 56 recycling recycling NN cord-328082-7c7slfbp 366 57 pathway pathway NN cord-328082-7c7slfbp 366 58 Forskolin forskolin NN cord-328082-7c7slfbp 366 59 inhibits inhibit VBZ cord-328082-7c7slfbp 366 60 and and CC cord-328082-7c7slfbp 366 61 reverses reverse VBZ cord-328082-7c7slfbp 366 62 the the DT cord-328082-7c7slfbp 366 63 effect effect NN cord-328082-7c7slfbp 366 64 of of IN cord-328082-7c7slfbp 366 65 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 366 66 A A NNP cord-328082-7c7slfbp 366 67 on on IN cord-328082-7c7slfbp 366 68 Golgi Golgi NNP cord-328082-7c7slfbp 366 69 morphology morphology NN cord-328082-7c7slfbp 366 70 by by IN cord-328082-7c7slfbp 366 71 a a DT cord-328082-7c7slfbp 366 72 cAMP camp NN cord-328082-7c7slfbp 366 73 - - HYPH cord-328082-7c7slfbp 366 74 independent independent JJ cord-328082-7c7slfbp 366 75 mechanism mechanism NN cord-328082-7c7slfbp 367 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 367 2 A A NNP cord-328082-7c7slfbp 367 3 's 's POS cord-328082-7c7slfbp 367 4 effects effect NNS cord-328082-7c7slfbp 367 5 on on IN cord-328082-7c7slfbp 367 6 endosomes endosome NNS cord-328082-7c7slfbp 367 7 , , , cord-328082-7c7slfbp 367 8 lysosomes lysosome NNS cord-328082-7c7slfbp 367 9 and and CC cord-328082-7c7slfbp 367 10 TGN TGN NNP cord-328082-7c7slfbp 367 11 suggest suggest VBP cord-328082-7c7slfbp 367 12 a a DT cord-328082-7c7slfbp 367 13 general general JJ cord-328082-7c7slfbp 367 14 mechanism mechanism NN cord-328082-7c7slfbp 367 15 for for IN cord-328082-7c7slfbp 367 16 regulating regulate VBG cord-328082-7c7slfbp 367 17 organelle organelle NN cord-328082-7c7slfbp 367 18 structure structure NN cord-328082-7c7slfbp 367 19 and and CC cord-328082-7c7slfbp 367 20 membrane membrane NN cord-328082-7c7slfbp 367 21 traffic traffic NN cord-328082-7c7slfbp 367 22 Perturbation perturbation NN cord-328082-7c7slfbp 367 23 of of IN cord-328082-7c7slfbp 367 24 cellular cellular JJ cord-328082-7c7slfbp 367 25 calcium calcium NN cord-328082-7c7slfbp 367 26 blocks block VBZ cord-328082-7c7slfbp 367 27 exit exit NN cord-328082-7c7slfbp 367 28 of of IN cord-328082-7c7slfbp 367 29 secretory secretory JJ cord-328082-7c7slfbp 367 30 proteins protein NNS cord-328082-7c7slfbp 367 31 from from IN cord-328082-7c7slfbp 367 32 the the DT cord-328082-7c7slfbp 367 33 rough rough JJ cord-328082-7c7slfbp 367 34 endoplasmic endoplasmic NN cord-328082-7c7slfbp 367 35 reticulum reticulum NN cord-328082-7c7slfbp 367 36 Antibodies Antibodies NNP cord-328082-7c7slfbp 367 37 to to IN cord-328082-7c7slfbp 367 38 Golgi Golgi NNP cord-328082-7c7slfbp 367 39 complex complex NN cord-328082-7c7slfbp 367 40 and and CC cord-328082-7c7slfbp 367 41 to to TO cord-328082-7c7slfbp 367 42 endoplasmic endoplasmic VB cord-328082-7c7slfbp 367 43 reticulum reticulum NN cord-328082-7c7slfbp 367 44 Okadaic Okadaic NNP cord-328082-7c7slfbp 367 45 acid acid NN cord-328082-7c7slfbp 367 46 induces induce VBZ cord-328082-7c7slfbp 367 47 Golgi Golgi NNP cord-328082-7c7slfbp 367 48 apparatus apparatus NN cord-328082-7c7slfbp 367 49 fragmentation fragmentation NN cord-328082-7c7slfbp 367 50 and and CC cord-328082-7c7slfbp 367 51 arrest arrest NN cord-328082-7c7slfbp 367 52 of of IN cord-328082-7c7slfbp 367 53 intracellular intracellular JJ cord-328082-7c7slfbp 367 54 transport transport NN cord-328082-7c7slfbp 368 1 The the DT cord-328082-7c7slfbp 368 2 El El NNP cord-328082-7c7slfbp 368 3 glycoprotein glycoprotein NN cord-328082-7c7slfbp 368 4 of of IN cord-328082-7c7slfbp 368 5 an an DT cord-328082-7c7slfbp 368 6 avian avian JJ cord-328082-7c7slfbp 368 7 coronavirus coronavirus NN cord-328082-7c7slfbp 368 8 is be VBZ cord-328082-7c7slfbp 368 9 targeted target VBN cord-328082-7c7slfbp 368 10 to to IN cord-328082-7c7slfbp 368 11 cis cis NNP cord-328082-7c7slfbp 368 12 - - HYPH cord-328082-7c7slfbp 368 13 Golgi Golgi NNP cord-328082-7c7slfbp 368 14 complex complex NN cord-328082-7c7slfbp 368 15 Reduced reduce VBN cord-328082-7c7slfbp 368 16 temperature temperature NN cord-328082-7c7slfbp 368 17 prevents prevent VBZ cord-328082-7c7slfbp 368 18 transfer transfer NN cord-328082-7c7slfbp 368 19 of of IN cord-328082-7c7slfbp 368 20 a a DT cord-328082-7c7slfbp 368 21 membrane membrane NN cord-328082-7c7slfbp 368 22 glycoprotein glycoprotein NN cord-328082-7c7slfbp 368 23 to to IN cord-328082-7c7slfbp 368 24 the the DT cord-328082-7c7slfbp 368 25 cell cell NN cord-328082-7c7slfbp 368 26 surface surface NN cord-328082-7c7slfbp 368 27 but but CC cord-328082-7c7slfbp 368 28 does do VBZ cord-328082-7c7slfbp 368 29 not not RB cord-328082-7c7slfbp 368 30 prevent prevent VB cord-328082-7c7slfbp 369 1 terminal terminal NNP cord-328082-7c7slfbp 369 2 glycolysation glycolysation NN cord-328082-7c7slfbp 369 3 Reduced reduce VBN cord-328082-7c7slfbp 369 4 extracellular extracellular JJ cord-328082-7c7slfbp 369 5 pH ph NN cord-328082-7c7slfbp 369 6 reversibly reversibly RB cord-328082-7c7slfbp 369 7 inhibits inhibit VBZ cord-328082-7c7slfbp 369 8 oligomerization oligomerization NN cord-328082-7c7slfbp 369 9 , , , cord-328082-7c7slfbp 369 10 intracellular intracellular JJ cord-328082-7c7slfbp 369 11 transport transport NN cord-328082-7c7slfbp 369 12 , , , cord-328082-7c7slfbp 369 13 and and CC cord-328082-7c7slfbp 369 14 processing processing NN cord-328082-7c7slfbp 369 15 of of IN cord-328082-7c7slfbp 369 16 the the DT cord-328082-7c7slfbp 369 17 influenza influenza NN cord-328082-7c7slfbp 369 18 hemagglutinin hemagglutinin NN cord-328082-7c7slfbp 369 19 in in IN cord-328082-7c7slfbp 369 20 infected infected JJ cord-328082-7c7slfbp 369 21 Madin Madin NNP cord-328082-7c7slfbp 369 22 - - HYPH cord-328082-7c7slfbp 369 23 Darby Darby NNP cord-328082-7c7slfbp 369 24 canine canine JJ cord-328082-7c7slfbp 369 25 kidney kidney NN cord-328082-7c7slfbp 369 26 cells cell NNS cord-328082-7c7slfbp 369 27 Periodate Periodate NNP cord-328082-7c7slfbp 369 28 - - HYPH cord-328082-7c7slfbp 369 29 lysine lysine NN cord-328082-7c7slfbp 369 30 - - HYPH cord-328082-7c7slfbp 369 31 paraformaldehyde paraformaldehyde NN cord-328082-7c7slfbp 369 32 fixative fixative NN cord-328082-7c7slfbp 369 33 . . . cord-328082-7c7slfbp 370 1 A a DT cord-328082-7c7slfbp 370 2 new new JJ cord-328082-7c7slfbp 370 3 fixative fixative NN cord-328082-7c7slfbp 370 4 for for IN cord-328082-7c7slfbp 370 5 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 370 6 microscopy microscopy NN cord-328082-7c7slfbp 370 7 Identification identification NN cord-328082-7c7slfbp 370 8 ofa ofa NN cord-328082-7c7slfbp 370 9 200-kD 200-kd CD cord-328082-7c7slfbp 370 10 , , , cord-328082-7c7slfbp 370 11 brefeldin brefeldin NNP cord-328082-7c7slfbp 370 12 - - HYPH cord-328082-7c7slfbp 370 13 sensitive sensitive JJ cord-328082-7c7slfbp 370 14 protein protein NN cord-328082-7c7slfbp 370 15 on on IN cord-328082-7c7slfbp 370 16 Golgi Golgi NNP cord-328082-7c7slfbp 370 17 membranes membrane NNS cord-328082-7c7slfbp 370 18 Identification identification NN cord-328082-7c7slfbp 370 19 of of IN cord-328082-7c7slfbp 370 20 23 23 CD cord-328082-7c7slfbp 370 21 complementation complementation NN cord-328082-7c7slfbp 370 22 groups group NNS cord-328082-7c7slfbp 370 23 required require VBN cord-328082-7c7slfbp 370 24 for for IN cord-328082-7c7slfbp 370 25 post post JJ cord-328082-7c7slfbp 370 26 - - JJ cord-328082-7c7slfbp 370 27 translational translational JJ cord-328082-7c7slfbp 370 28 events event NNS cord-328082-7c7slfbp 370 29 in in IN cord-328082-7c7slfbp 370 30 the the DT cord-328082-7c7slfbp 370 31 yeast yeast NN cord-328082-7c7slfbp 370 32 secretory secretory NN cord-328082-7c7slfbp 370 33 pathway pathway NN cord-328082-7c7slfbp 370 34 Evidence evidence NN cord-328082-7c7slfbp 370 35 that that IN cord-328082-7c7slfbp 370 36 luminal luminal JJ cord-328082-7c7slfbp 370 37 ER er NN cord-328082-7c7slfbp 370 38 proteins protein NNS cord-328082-7c7slfbp 370 39 are be VBP cord-328082-7c7slfbp 370 40 sorted sort VBN cord-328082-7c7slfbp 370 41 from from IN cord-328082-7c7slfbp 370 42 secreted secreted JJ cord-328082-7c7slfbp 370 43 proteins protein NNS cord-328082-7c7slfbp 370 44 in in IN cord-328082-7c7slfbp 370 45 a a DT cord-328082-7c7slfbp 370 46 post post JJ cord-328082-7c7slfbp 370 47 - - JJ cord-328082-7c7slfbp 370 48 ER er JJ cord-328082-7c7slfbp 370 49 compartment compartment NN cord-328082-7c7slfbp 370 50 Control control NN cord-328082-7c7slfbp 370 51 of of IN cord-328082-7c7slfbp 370 52 protein protein NN cord-328082-7c7slfbp 370 53 exit exit NN cord-328082-7c7slfbp 370 54 from from IN cord-328082-7c7slfbp 370 55 the the DT cord-328082-7c7slfbp 370 56 endoplasmic endoplasmic NN cord-328082-7c7slfbp 370 57 reticulum reticulum NN cord-328082-7c7slfbp 370 58 Multiple multiple JJ cord-328082-7c7slfbp 370 59 targets target NNS cord-328082-7c7slfbp 370 60 for for IN cord-328082-7c7slfbp 370 61 BFA BFA NNP cord-328082-7c7slfbp 370 62 Rablb Rablb NNP cord-328082-7c7slfbp 370 63 regulates regulate VBZ cord-328082-7c7slfbp 370 64 vesicular vesicular JJ cord-328082-7c7slfbp 370 65 transport transport NN cord-328082-7c7slfbp 370 66 between between IN cord-328082-7c7slfbp 370 67 the the DT cord-328082-7c7slfbp 370 68 endoplasmic endoplasmic NN cord-328082-7c7slfbp 370 69 reticulum reticulum NN cord-328082-7c7slfbp 370 70 and and CC cord-328082-7c7slfbp 370 71 successive successive JJ cord-328082-7c7slfbp 370 72 Golgi Golgi NNP cord-328082-7c7slfbp 370 73 compartments compartment NNS cord-328082-7c7slfbp 370 74 Perturbation perturbation NN cord-328082-7c7slfbp 370 75 of of IN cord-328082-7c7slfbp 370 76 the the DT cord-328082-7c7slfbp 370 77 morphology morphology NN cord-328082-7c7slfbp 370 78 of of IN cord-328082-7c7slfbp 370 79 the the DT cord-328082-7c7slfbp 370 80 trans trans NNP cord-328082-7c7slfbp 370 81 - - NNP cord-328082-7c7slfbp 370 82 Oolgi Oolgi NNP cord-328082-7c7slfbp 370 83 network network NN cord-328082-7c7slfbp 370 84 following follow VBG cord-328082-7c7slfbp 370 85 brefeldin brefeldin NNP cord-328082-7c7slfbp 371 1 A a DT cord-328082-7c7slfbp 371 2 treatment treatment NN cord-328082-7c7slfbp 371 3 : : : cord-328082-7c7slfbp 371 4 redistribution redistribution NN cord-328082-7c7slfbp 371 5 of of IN cord-328082-7c7slfbp 371 6 a a DT cord-328082-7c7slfbp 371 7 TGNspecific TGNspecific NNP cord-328082-7c7slfbp 371 8 integral integral JJ cord-328082-7c7slfbp 371 9 membrane membrane NN cord-328082-7c7slfbp 371 10 protein protein NN cord-328082-7c7slfbp 371 11 Regulation regulation NN cord-328082-7c7slfbp 371 12 of of IN cord-328082-7c7slfbp 371 13 protein protein NN cord-328082-7c7slfbp 371 14 export export NN cord-328082-7c7slfbp 371 15 from from IN cord-328082-7c7slfbp 371 16 the the DT cord-328082-7c7slfbp 371 17 endoplasmic endoplasmic NN cord-328082-7c7slfbp 371 18 reticulum reticulum NN cord-328082-7c7slfbp 371 19 Molecular molecular JJ cord-328082-7c7slfbp 371 20 dissection dissection NN cord-328082-7c7slfbp 371 21 of of IN cord-328082-7c7slfbp 371 22 the the DT cord-328082-7c7slfbp 371 23 secretory secretory JJ cord-328082-7c7slfbp 371 24 pathway pathway NN cord-328082-7c7slfbp 371 25 Reconstitution Reconstitution NNP cord-328082-7c7slfbp 371 26 of of IN cord-328082-7c7slfbp 371 27 protein protein NN cord-328082-7c7slfbp 371 28 transport transport NN cord-328082-7c7slfbp 371 29 from from IN cord-328082-7c7slfbp 371 30 the the DT cord-328082-7c7slfbp 371 31 endoplasmic endoplasmic NN cord-328082-7c7slfbp 371 32 reticulum reticulum NN cord-328082-7c7slfbp 371 33 to to IN cord-328082-7c7slfbp 371 34 the the DT cord-328082-7c7slfbp 371 35 Golgi Golgi NNP cord-328082-7c7slfbp 371 36 complex complex NN cord-328082-7c7slfbp 371 37 in in IN cord-328082-7c7slfbp 371 38 yeast yeast NN cord-328082-7c7slfbp 371 39 : : : cord-328082-7c7slfbp 371 40 the the DT cord-328082-7c7slfbp 371 41 acceptor acceptor NN cord-328082-7c7slfbp 371 42 Golgi Golgi NNP cord-328082-7c7slfbp 371 43 compartment compartment NN cord-328082-7c7slfbp 371 44 is be VBZ cord-328082-7c7slfbp 371 45 defective defective JJ cord-328082-7c7slfbp 371 46 in in IN cord-328082-7c7slfbp 371 47 the the DT cord-328082-7c7slfbp 371 48 sec23 sec23 CD cord-328082-7c7slfbp 371 49 mutant mutant NN cord-328082-7c7slfbp 372 1 The the DT cord-328082-7c7slfbp 372 2 involvement involvement NN cord-328082-7c7slfbp 372 3 of of IN cord-328082-7c7slfbp 372 4 calcium calcium NN cord-328082-7c7slfbp 372 5 in in IN cord-328082-7c7slfbp 372 6 transport transport NN cord-328082-7c7slfbp 372 7 of of IN cord-328082-7c7slfbp 372 8 secretory secretory JJ cord-328082-7c7slfbp 372 9 proteins protein NNS cord-328082-7c7slfbp 372 10 from from IN cord-328082-7c7slfbp 372 11 the the DT cord-328082-7c7slfbp 372 12 endoplasmic endoplasmic NN cord-328082-7c7slfbp 372 13 reticulum reticulum NN cord-328082-7c7slfbp 372 14 Pre Pre NNP cord-328082-7c7slfbp 372 15 - - : cord-328082-7c7slfbp 372 16 and and CC cord-328082-7c7slfbp 372 17 post post AFX cord-328082-7c7slfbp 372 18 - - JJ cord-328082-7c7slfbp 372 19 Oolgi oolgi JJ cord-328082-7c7slfbp 372 20 vacuoles vacuole NNS cord-328082-7c7slfbp 372 21 operate operate VBP cord-328082-7c7slfbp 372 22 in in IN cord-328082-7c7slfbp 372 23 the the DT cord-328082-7c7slfbp 372 24 transport transport NN cord-328082-7c7slfbp 372 25 of of IN cord-328082-7c7slfbp 372 26 Semliki Semliki NNP cord-328082-7c7slfbp 372 27 forest forest NN cord-328082-7c7slfbp 372 28 virus virus NN cord-328082-7c7slfbp 372 29 membrane membrane NN cord-328082-7c7slfbp 372 30 glycoproteins glycoprotein NNS cord-328082-7c7slfbp 372 31 to to IN cord-328082-7c7slfbp 372 32 the the DT cord-328082-7c7slfbp 372 33 cell cell NN cord-328082-7c7slfbp 372 34 surface surface NN cord-328082-7c7slfbp 372 35 Pathways pathway NNS cord-328082-7c7slfbp 372 36 of of IN cord-328082-7c7slfbp 372 37 protein protein NN cord-328082-7c7slfbp 372 38 sorting sorting NN cord-328082-7c7slfbp 372 39 and and CC cord-328082-7c7slfbp 372 40 membrane membrane NN cord-328082-7c7slfbp 372 41 traffic traffic NN cord-328082-7c7slfbp 372 42 between between IN cord-328082-7c7slfbp 372 43 the the DT cord-328082-7c7slfbp 372 44 rough rough JJ cord-328082-7c7slfbp 372 45 endoplasmic endoplasmic NN cord-328082-7c7slfbp 372 46 reticulum reticulum NN cord-328082-7c7slfbp 372 47 and and CC cord-328082-7c7slfbp 372 48 the the DT cord-328082-7c7slfbp 372 49 Golgi Golgi NNP cord-328082-7c7slfbp 372 50 complex complex NN cord-328082-7c7slfbp 372 51 Distribution distribution NN cord-328082-7c7slfbp 372 52 of of IN cord-328082-7c7slfbp 372 53 the the DT cord-328082-7c7slfbp 372 54 intermediate intermediate JJ cord-328082-7c7slfbp 372 55 elements element NNS cord-328082-7c7slfbp 372 56 operating operate VBG cord-328082-7c7slfbp 372 57 in in IN cord-328082-7c7slfbp 372 58 ER ER NNP cord-328082-7c7slfbp 372 59 to to IN cord-328082-7c7slfbp 372 60 Golgi Golgi NNP cord-328082-7c7slfbp 372 61 transport transport NN cord-328082-7c7slfbp 372 62 Semliki Semliki NNP cord-328082-7c7slfbp 372 63 Forest Forest NNP cord-328082-7c7slfbp 372 64 virus virus NN cord-328082-7c7slfbp 372 65 mutants mutant NNS cord-328082-7c7slfbp 372 66 with with IN cord-328082-7c7slfbp 372 67 temperature temperature NN cord-328082-7c7slfbp 372 68 - - HYPH cord-328082-7c7slfbp 372 69 sensitive sensitive JJ cord-328082-7c7slfbp 372 70 transport transport NN cord-328082-7c7slfbp 372 71 defect defect NN cord-328082-7c7slfbp 372 72 of of IN cord-328082-7c7slfbp 372 73 envelope envelope NN cord-328082-7c7slfbp 372 74 proteins protein NNS cord-328082-7c7slfbp 372 75 Antibodies antibody NNS cord-328082-7c7slfbp 372 76 to to IN cord-328082-7c7slfbp 372 77 rat rat NN cord-328082-7c7slfbp 372 78 pancreas pancreas NN cord-328082-7c7slfbp 372 79 Golgi Golgi NNP cord-328082-7c7slfbp 372 80 subfractions subfraction NNS cord-328082-7c7slfbp 372 81 : : : cord-328082-7c7slfbp 372 82 identification identification NN cord-328082-7c7slfbp 372 83 of of IN cord-328082-7c7slfbp 372 84 a a DT cord-328082-7c7slfbp 372 85 58-kD 58-kd CD cord-328082-7c7slfbp 372 86 cis cis NNP cord-328082-7c7slfbp 372 87 - - HYPH cord-328082-7c7slfbp 372 88 Golgi Golgi NNP cord-328082-7c7slfbp 372 89 protein protein NN cord-328082-7c7slfbp 372 90 Caffeine Caffeine NNP cord-328082-7c7slfbp 372 91 - - HYPH cord-328082-7c7slfbp 372 92 induced induce VBN cord-328082-7c7slfbp 372 93 uncoupling uncoupling NN cord-328082-7c7slfbp 372 94 of of IN cord-328082-7c7slfbp 372 95 mitosis mitosis NN cord-328082-7c7slfbp 372 96 from from IN cord-328082-7c7slfbp 372 97 the the DT cord-328082-7c7slfbp 372 98 completion completion NN cord-328082-7c7slfbp 372 99 of of IN cord-328082-7c7slfbp 372 100 DNA dna NN cord-328082-7c7slfbp 372 101 replication replication NN cord-328082-7c7slfbp 372 102 in in IN cord-328082-7c7slfbp 372 103 mammalian mammalian JJ cord-328082-7c7slfbp 372 104 cells cell NNS cord-328082-7c7slfbp 372 105 Identification identification NN cord-328082-7c7slfbp 372 106 , , , cord-328082-7c7slfbp 372 107 by by IN cord-328082-7c7slfbp 372 108 a a DT cord-328082-7c7slfbp 372 109 monoclonal monoclonal JJ cord-328082-7c7slfbp 372 110 antibody antibody NN cord-328082-7c7slfbp 372 111 , , , cord-328082-7c7slfbp 372 112 of of IN cord-328082-7c7slfbp 372 113 a a DT cord-328082-7c7slfbp 372 114 53-kD 53-kd CD cord-328082-7c7slfbp 372 115 protein protein NN cord-328082-7c7slfbp 372 116 associated associate VBN cord-328082-7c7slfbp 372 117 with with IN cord-328082-7c7slfbp 372 118 a a DT cord-328082-7c7slfbp 372 119 tubulo tubulo JJ cord-328082-7c7slfbp 372 120 - - HYPH cord-328082-7c7slfbp 372 121 vesicular vesicular JJ cord-328082-7c7slfbp 372 122 compartment compartment NN cord-328082-7c7slfbp 372 123 at at IN cord-328082-7c7slfbp 372 124 the the DT cord-328082-7c7slfbp 372 125 cis cis NNP cord-328082-7c7slfbp 372 126 - - HYPH cord-328082-7c7slfbp 372 127 side side NN cord-328082-7c7slfbp 372 128 of of IN cord-328082-7c7slfbp 372 129 the the DT cord-328082-7c7slfbp 372 130 Golgi Golgi NNP cord-328082-7c7slfbp 372 131 apparatus apparatus NN cord-328082-7c7slfbp 373 1 A a DT cord-328082-7c7slfbp 373 2 heterotrimeric heterotrimeric JJ cord-328082-7c7slfbp 373 3 G g NN cord-328082-7c7slfbp 373 4 protein protein NN cord-328082-7c7slfbp 373 5 , , , cord-328082-7c7slfbp 373 6 G~_3 G~_3 NNP cord-328082-7c7slfbp 373 7 , , , cord-328082-7c7slfbp 373 8 on on IN cord-328082-7c7slfbp 373 9 Golgi Golgi NNP cord-328082-7c7slfbp 373 10 membranes membrane NNS cord-328082-7c7slfbp 373 11 regulates regulate VBZ cord-328082-7c7slfbp 373 12 the the DT cord-328082-7c7slfbp 373 13 secretion secretion NN cord-328082-7c7slfbp 373 14 of of IN cord-328082-7c7slfbp 373 15 a a DT cord-328082-7c7slfbp 373 16 heparan heparan JJ cord-328082-7c7slfbp 373 17 sulfate sulfate NN cord-328082-7c7slfbp 373 18 proteoglycan proteoglycan NN cord-328082-7c7slfbp 373 19 in in IN cord-328082-7c7slfbp 373 20 LLC LLC NNP cord-328082-7c7slfbp 373 21 - - HYPH cord-328082-7c7slfbp 373 22 PK~ PK~ NNP cord-328082-7c7slfbp 373 23 cells cell NNS cord-328082-7c7slfbp 374 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 374 2 A A NNP cord-328082-7c7slfbp 374 3 induces induce VBZ cord-328082-7c7slfbp 374 4 a a DT cord-328082-7c7slfbp 374 5 microtubule microtubule NN cord-328082-7c7slfbp 374 6 - - HYPH cord-328082-7c7slfbp 374 7 dependent dependent JJ cord-328082-7c7slfbp 374 8 fusion fusion NN cord-328082-7c7slfbp 374 9 of of IN cord-328082-7c7slfbp 374 10 galactosyltransferase galactosyltransferase NN cord-328082-7c7slfbp 374 11 - - HYPH cord-328082-7c7slfbp 374 12 containing contain VBG cord-328082-7c7slfbp 374 13 vesicles vesicle NNS cord-328082-7c7slfbp 374 14 with with IN cord-328082-7c7slfbp 374 15 the the DT cord-328082-7c7slfbp 374 16 rough rough JJ cord-328082-7c7slfbp 374 17 endoplasmic endoplasmic NN cord-328082-7c7slfbp 374 18 reticulum reticulum NN cord-328082-7c7slfbp 374 19 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 374 20 A A NNP cord-328082-7c7slfbp 374 21 , , , cord-328082-7c7slfbp 374 22 a a DT cord-328082-7c7slfbp 374 23 specific specific JJ cord-328082-7c7slfbp 374 24 inhibitor inhibitor NN cord-328082-7c7slfbp 374 25 of of IN cord-328082-7c7slfbp 374 26 intracellular intracellular JJ cord-328082-7c7slfbp 374 27 translocation translocation NN cord-328082-7c7slfbp 374 28 of of IN cord-328082-7c7slfbp 374 29 vesicular vesicular JJ cord-328082-7c7slfbp 374 30 stomatitis stomatitis NN cord-328082-7c7slfbp 374 31 virus virus NN cord-328082-7c7slfbp 374 32 G g NN cord-328082-7c7slfbp 374 33 protein protein NN cord-328082-7c7slfbp 374 34 : : : cord-328082-7c7slfbp 375 1 intracellular intracellular JJ cord-328082-7c7slfbp 375 2 accumulation accumulation NN cord-328082-7c7slfbp 375 3 of of IN cord-328082-7c7slfbp 375 4 high high JJ cord-328082-7c7slfbp 375 5 - - HYPH cord-328082-7c7slfbp 375 6 mannose mannose NN cord-328082-7c7slfbp 375 7 type type NN cord-328082-7c7slfbp 375 8 G g NN cord-328082-7c7slfbp 375 9 protein protein NN cord-328082-7c7slfbp 375 10 and and CC cord-328082-7c7slfbp 375 11 inhibition inhibition NN cord-328082-7c7slfbp 375 12 of of IN cord-328082-7c7slfbp 375 13 its -PRON- PRP$ cord-328082-7c7slfbp 375 14 cell cell NN cord-328082-7c7slfbp 375 15 surface surface NN cord-328082-7c7slfbp 375 16 expression expression NN cord-328082-7c7slfbp 375 17 Retrograde retrograde JJ cord-328082-7c7slfbp 375 18 transport transport NN cord-328082-7c7slfbp 375 19 from from IN cord-328082-7c7slfbp 375 20 the the DT cord-328082-7c7slfbp 375 21 Golgi Golgi NNP cord-328082-7c7slfbp 375 22 region region NN cord-328082-7c7slfbp 375 23 to to IN cord-328082-7c7slfbp 375 24 the the DT cord-328082-7c7slfbp 375 25 endoplasmic endoplasmic NN cord-328082-7c7slfbp 375 26 reticulum reticulum NN cord-328082-7c7slfbp 375 27 is be VBZ cord-328082-7c7slfbp 375 28 sensitive sensitive JJ cord-328082-7c7slfbp 375 29 to to IN cord-328082-7c7slfbp 375 30 GTP'rS GTP'rS NNP cord-328082-7c7slfbp 375 31 Temperature temperature NN cord-328082-7c7slfbp 375 32 and and CC cord-328082-7c7slfbp 375 33 energy energy NN cord-328082-7c7slfbp 375 34 dependence dependence NN cord-328082-7c7slfbp 375 35 of of IN cord-328082-7c7slfbp 375 36 secretory secretory JJ cord-328082-7c7slfbp 375 37 protein protein NN cord-328082-7c7slfbp 375 38 transport transport NN cord-328082-7c7slfbp 375 39 in in IN cord-328082-7c7slfbp 375 40 the the DT cord-328082-7c7slfbp 375 41 exocrine exocrine JJ cord-328082-7c7slfbp 375 42 pancreas pancreas NN cord-328082-7c7slfbp 375 43 Microtubules microtubule NNS cord-328082-7c7slfbp 375 44 and and CC cord-328082-7c7slfbp 375 45 the the DT cord-328082-7c7slfbp 375 46 organization organization NN cord-328082-7c7slfbp 375 47 of of IN cord-328082-7c7slfbp 375 48 the the DT cord-328082-7c7slfbp 375 49 Golgi Golgi NNP cord-328082-7c7slfbp 375 50 complex complex NN cord-328082-7c7slfbp 376 1 Brefeldin Brefeldin NNP cord-328082-7c7slfbp 376 2 A A NNP cord-328082-7c7slfbp 376 3 causes cause VBZ cord-328082-7c7slfbp 376 4 a a DT cord-328082-7c7slfbp 376 5 microtubule microtubule NN cord-328082-7c7slfbp 376 6 - - HYPH cord-328082-7c7slfbp 376 7 mediated mediate VBN cord-328082-7c7slfbp 376 8 fusion fusion NN cord-328082-7c7slfbp 376 9 of of IN cord-328082-7c7slfbp 376 10 the the DT cord-328082-7c7slfbp 376 11 trans trans NNP cord-328082-7c7slfbp 376 12 - - NNP cord-328082-7c7slfbp 376 13 Golgi Golgi NNP cord-328082-7c7slfbp 376 14 network network NN cord-328082-7c7slfbp 376 15 and and CC cord-328082-7c7slfbp 376 16 early early JJ cord-328082-7c7slfbp 376 17 endosomes endosome NNS cord-328082-7c7slfbp 377 1 Many many JJ cord-328082-7c7slfbp 377 2 thanks thank NNS cord-328082-7c7slfbp 377 3 go go VBP cord-328082-7c7slfbp 377 4 to to IN cord-328082-7c7slfbp 377 5 Drs Drs NNP cord-328082-7c7slfbp 377 6 . . . cord-328082-7c7slfbp 378 1 J. J. NNP cord-328082-7c7slfbp 378 2 Saraste Saraste NNP cord-328082-7c7slfbp 378 3 and and CC cord-328082-7c7slfbp 378 4 K. K. NNP cord-328082-7c7slfbp 378 5 Hedman Hedman NNP cord-328082-7c7slfbp 378 6 for for IN cord-328082-7c7slfbp 378 7 advice advice NN cord-328082-7c7slfbp 378 8 to to IN cord-328082-7c7slfbp 378 9 J. J. NNP cord-328082-7c7slfbp 378 10 J J NNP cord-328082-7c7slfbp 378 11 / / SYM cord-328082-7c7slfbp 378 12 intti intti NNP cord-328082-7c7slfbp 378 13 with with IN cord-328082-7c7slfbp 378 14 the the DT cord-328082-7c7slfbp 378 15 immunoelectron immunoelectron NNP cord-328082-7c7slfbp 378 16 microscopic microscopic JJ cord-328082-7c7slfbp 378 17 technique technique NN cord-328082-7c7slfbp 378 18 . . . cord-328082-7c7slfbp 379 1 We -PRON- PRP cord-328082-7c7slfbp 379 2 thank thank VBP cord-328082-7c7slfbp 379 3 Jorma Jorma NNP cord-328082-7c7slfbp 379 4 Wartiovaara Wartiovaara NNP cord-328082-7c7slfbp 379 5 , , , cord-328082-7c7slfbp 379 6 head head NN cord-328082-7c7slfbp 379 7 of of IN cord-328082-7c7slfbp 379 8 the the DT cord-328082-7c7slfbp 379 9 Department Department NNP cord-328082-7c7slfbp 379 10 of of IN cord-328082-7c7slfbp 379 11 Electron Electron NNP cord-328082-7c7slfbp 379 12 Microscopy Microscopy NNP cord-328082-7c7slfbp 379 13 , , , cord-328082-7c7slfbp 379 14 University University NNP cord-328082-7c7slfbp 379 15 of of IN cord-328082-7c7slfbp 379 16 Helsinki Helsinki NNP cord-328082-7c7slfbp 379 17 , , , cord-328082-7c7slfbp 379 18 for for IN cord-328082-7c7slfbp 379 19 letting let VBG cord-328082-7c7slfbp 379 20 us -PRON- PRP cord-328082-7c7slfbp 379 21 use use VB cord-328082-7c7slfbp 379 22 the the DT cord-328082-7c7slfbp 379 23 facilities facility NNS cord-328082-7c7slfbp 379 24 of of IN cord-328082-7c7slfbp 379 25 his -PRON- PRP$ cord-328082-7c7slfbp 379 26 department department NN cord-328082-7c7slfbp 379 27 . . . cord-328082-7c7slfbp 380 1 Virpi Virpi NNP cord-328082-7c7slfbp 380 2 M M NNP cord-328082-7c7slfbp 380 3 / / SYM cord-328082-7c7slfbp 380 4 ikiranta ikiranta NN cord-328082-7c7slfbp 380 5 and and CC cord-328082-7c7slfbp 380 6 Mervi Mervi NNP cord-328082-7c7slfbp 380 7 Lindman Lindman NNP cord-328082-7c7slfbp 380 8 are be VBP cord-328082-7c7slfbp 380 9 acknowledged acknowledge VBN cord-328082-7c7slfbp 380 10 for for IN cord-328082-7c7slfbp 380 11 excellent excellent JJ cord-328082-7c7slfbp 380 12 technical technical JJ cord-328082-7c7slfbp 380 13 assistance assistance NN cord-328082-7c7slfbp 380 14 . . . cord-328082-7c7slfbp 381 1 Jaakko Jaakko NNP cord-328082-7c7slfbp 381 2 Saraste Saraste NNP cord-328082-7c7slfbp 381 3 , , , cord-328082-7c7slfbp 381 4 Mikael Mikael NNP cord-328082-7c7slfbp 381 5 Rehn Rehn NNP cord-328082-7c7slfbp 381 6 and and CC cord-328082-7c7slfbp 381 7 Camilla Camilla NNP cord-328082-7c7slfbp 381 8 Schalin Schalin NNP cord-328082-7c7slfbp 381 9 - - HYPH cord-328082-7c7slfbp 381 10 J J NNP cord-328082-7c7slfbp 382 1 ~intti ~intti NNP cord-328082-7c7slfbp 382 2 are be VBP cord-328082-7c7slfbp 382 3 acknowledged acknowledge VBN cord-328082-7c7slfbp 382 4 for for IN cord-328082-7c7slfbp 382 5 critically critically RB cord-328082-7c7slfbp 382 6 reading read VBG cord-328082-7c7slfbp 382 7 the the DT cord-328082-7c7slfbp 382 8 manuscript manuscript NN cord-328082-7c7slfbp 382 9 . . . cord-328082-7c7slfbp 383 1 This this DT cord-328082-7c7slfbp 383 2 study study NN cord-328082-7c7slfbp 383 3 was be VBD cord-328082-7c7slfbp 383 4 supported support VBN cord-328082-7c7slfbp 383 5 by by IN cord-328082-7c7slfbp 383 6 the the DT cord-328082-7c7slfbp 383 7 Academy Academy NNP cord-328082-7c7slfbp 383 8 of of IN cord-328082-7c7slfbp 383 9 Finland Finland NNP cord-328082-7c7slfbp 383 10 , , , cord-328082-7c7slfbp 383 11 the the DT cord-328082-7c7slfbp 383 12 Alfred Alfred NNP cord-328082-7c7slfbp 383 13 Kordelin Kordelin NNP cord-328082-7c7slfbp 383 14 Foundation Foundation NNP cord-328082-7c7slfbp 383 15 , , , cord-328082-7c7slfbp 383 16 the the DT cord-328082-7c7slfbp 383 17 Finnish Finnish NNP cord-328082-7c7slfbp 383 18 Cultural Cultural NNP cord-328082-7c7slfbp 383 19 Foundation Foundation NNP cord-328082-7c7slfbp 383 20 , , , cord-328082-7c7slfbp 383 21 Magnus Magnus NNP cord-328082-7c7slfbp 383 22 Ehrnrooth Ehrnrooth NNP cord-328082-7c7slfbp 383 23 Foundation Foundation NNP cord-328082-7c7slfbp 383 24 , , , cord-328082-7c7slfbp 383 25 and and CC cord-328082-7c7slfbp 383 26 the the DT cord-328082-7c7slfbp 383 27 University University NNP cord-328082-7c7slfbp 383 28 of of IN cord-328082-7c7slfbp 383 29 Helsinki Helsinki NNP cord-328082-7c7slfbp 383 30 . . .