id sid tid token lemma pos cord-319626-f1b3ygg0 1 1 key key JJ cord-319626-f1b3ygg0 2 1 : : : cord-319626-f1b3ygg0 2 2 cord-319626-f1b3ygg0 cord-319626-f1b3ygg0 NFP cord-319626-f1b3ygg0 2 3 authors author NNS cord-319626-f1b3ygg0 2 4 : : : cord-319626-f1b3ygg0 3 1 nan nan NNP cord-319626-f1b3ygg0 3 2 title title NN cord-319626-f1b3ygg0 3 3 : : : cord-319626-f1b3ygg0 3 4 Site site NN cord-319626-f1b3ygg0 3 5 of of IN cord-319626-f1b3ygg0 3 6 addition addition NN cord-319626-f1b3ygg0 3 7 of of IN cord-319626-f1b3ygg0 3 8 N n NN cord-319626-f1b3ygg0 3 9 - - HYPH cord-319626-f1b3ygg0 3 10 acetyl acetyl NN cord-319626-f1b3ygg0 3 11 - - HYPH cord-319626-f1b3ygg0 3 12 galactosamine galactosamine NN cord-319626-f1b3ygg0 3 13 to to IN cord-319626-f1b3ygg0 3 14 the the DT cord-319626-f1b3ygg0 3 15 E1 e1 NN cord-319626-f1b3ygg0 3 16 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 3 17 of of IN cord-319626-f1b3ygg0 3 18 mouse mouse NN cord-319626-f1b3ygg0 3 19 hepatitis hepatitis NN cord-319626-f1b3ygg0 3 20 virus virus NN cord-319626-f1b3ygg0 3 21 - - : cord-319626-f1b3ygg0 3 22 A59 A59 NNP cord-319626-f1b3ygg0 3 23 date date NN cord-319626-f1b3ygg0 3 24 : : : cord-319626-f1b3ygg0 3 25 1988 1988 CD cord-319626-f1b3ygg0 3 26 - - SYM cord-319626-f1b3ygg0 3 27 05 05 CD cord-319626-f1b3ygg0 3 28 - - HYPH cord-319626-f1b3ygg0 3 29 01 01 CD cord-319626-f1b3ygg0 3 30 journal journal NN cord-319626-f1b3ygg0 3 31 : : : cord-319626-f1b3ygg0 3 32 J J NNP cord-319626-f1b3ygg0 3 33 Cell Cell NNP cord-319626-f1b3ygg0 3 34 Biol Biol NNP cord-319626-f1b3ygg0 3 35 DOI DOI NNP cord-319626-f1b3ygg0 3 36 : : : cord-319626-f1b3ygg0 4 1 nan nan NNP cord-319626-f1b3ygg0 4 2 sha sha NNP cord-319626-f1b3ygg0 4 3 : : : cord-319626-f1b3ygg0 4 4 bbccfb72296dcfb544b20996414b7b70622214da bbccfb72296dcfb544b20996414b7b70622214da NNP cord-319626-f1b3ygg0 4 5 doc_id doc_id CD cord-319626-f1b3ygg0 4 6 : : : cord-319626-f1b3ygg0 4 7 319626 319626 CD cord-319626-f1b3ygg0 4 8 cord_uid cord_uid NNS cord-319626-f1b3ygg0 4 9 : : : cord-319626-f1b3ygg0 4 10 f1b3ygg0 f1b3ygg0 ADD cord-319626-f1b3ygg0 5 1 By by IN cord-319626-f1b3ygg0 5 2 pulse pulse NN cord-319626-f1b3ygg0 5 3 - - HYPH cord-319626-f1b3ygg0 5 4 chase chase NN cord-319626-f1b3ygg0 5 5 labeling labeling NN cord-319626-f1b3ygg0 5 6 with with IN cord-319626-f1b3ygg0 5 7 [ [ -LRB- cord-319626-f1b3ygg0 5 8 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 5 9 and and CC cord-319626-f1b3ygg0 5 10 long long JJ cord-319626-f1b3ygg0 5 11 - - HYPH cord-319626-f1b3ygg0 5 12 term term NN cord-319626-f1b3ygg0 5 13 labeling labeling NN cord-319626-f1b3ygg0 5 14 with with IN cord-319626-f1b3ygg0 5 15 3H 3h CD cord-319626-f1b3ygg0 5 16 - - HYPH cord-319626-f1b3ygg0 5 17 sugars sugar NNS cord-319626-f1b3ygg0 5 18 , , , cord-319626-f1b3ygg0 5 19 the the DT cord-319626-f1b3ygg0 5 20 E1 e1 NN cord-319626-f1b3ygg0 5 21 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 5 22 of of IN cord-319626-f1b3ygg0 5 23 coronavirus coronavirus NN cord-319626-f1b3ygg0 5 24 MHV MHV NNP cord-319626-f1b3ygg0 5 25 - - HYPH cord-319626-f1b3ygg0 5 26 A59 A59 NNP cord-319626-f1b3ygg0 5 27 has have VBZ cord-319626-f1b3ygg0 5 28 been be VBN cord-319626-f1b3ygg0 5 29 shown show VBN cord-319626-f1b3ygg0 5 30 to to TO cord-319626-f1b3ygg0 5 31 acquire acquire VB cord-319626-f1b3ygg0 5 32 O o NN cord-319626-f1b3ygg0 5 33 - - HYPH cord-319626-f1b3ygg0 5 34 linked link VBN cord-319626-f1b3ygg0 5 35 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 5 36 in in IN cord-319626-f1b3ygg0 5 37 a a DT cord-319626-f1b3ygg0 5 38 two two CD cord-319626-f1b3ygg0 5 39 - - HYPH cord-319626-f1b3ygg0 5 40 step step NN cord-319626-f1b3ygg0 5 41 process process NN cord-319626-f1b3ygg0 5 42 . . . cord-319626-f1b3ygg0 6 1 About about RB cord-319626-f1b3ygg0 6 2 10 10 CD cord-319626-f1b3ygg0 6 3 min min NN cord-319626-f1b3ygg0 6 4 after after IN cord-319626-f1b3ygg0 6 5 synthesis synthesis NN cord-319626-f1b3ygg0 6 6 of of IN cord-319626-f1b3ygg0 6 7 the the DT cord-319626-f1b3ygg0 6 8 E1 E1 NNP cord-319626-f1b3ygg0 6 9 protein protein NN cord-319626-f1b3ygg0 6 10 , , , cord-319626-f1b3ygg0 6 11 N N NNP cord-319626-f1b3ygg0 6 12 - - HYPH cord-319626-f1b3ygg0 6 13 acetyl acetyl NNP cord-319626-f1b3ygg0 6 14 - - HYPH cord-319626-f1b3ygg0 6 15 galactosamine galactosamine NNP cord-319626-f1b3ygg0 6 16 was be VBD cord-319626-f1b3ygg0 6 17 added add VBN cord-319626-f1b3ygg0 6 18 . . . cord-319626-f1b3ygg0 7 1 This this DT cord-319626-f1b3ygg0 7 2 was be VBD cord-319626-f1b3ygg0 7 3 followed follow VBN cord-319626-f1b3ygg0 7 4 approximately approximately RB cord-319626-f1b3ygg0 7 5 10 10 CD cord-319626-f1b3ygg0 7 6 min min NN cord-319626-f1b3ygg0 7 7 later later RB cord-319626-f1b3ygg0 7 8 by by IN cord-319626-f1b3ygg0 7 9 the the DT cord-319626-f1b3ygg0 7 10 addition addition NN cord-319626-f1b3ygg0 7 11 of of IN cord-319626-f1b3ygg0 7 12 both both CC cord-319626-f1b3ygg0 7 13 galactose galactose NN cord-319626-f1b3ygg0 7 14 and and CC cord-319626-f1b3ygg0 7 15 sialic sialic NN cord-319626-f1b3ygg0 7 16 acid acid NN cord-319626-f1b3ygg0 7 17 to to TO cord-319626-f1b3ygg0 7 18 give give VB cord-319626-f1b3ygg0 7 19 the the DT cord-319626-f1b3ygg0 7 20 mature mature JJ cord-319626-f1b3ygg0 7 21 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 7 22 . . . cord-319626-f1b3ygg0 8 1 This this DT cord-319626-f1b3ygg0 8 2 sequence sequence NN cord-319626-f1b3ygg0 8 3 of of IN cord-319626-f1b3ygg0 8 4 additions addition NNS cord-319626-f1b3ygg0 8 5 was be VBD cord-319626-f1b3ygg0 8 6 confirmed confirm VBN cord-319626-f1b3ygg0 8 7 by by IN cord-319626-f1b3ygg0 8 8 analyzing analyze VBG cord-319626-f1b3ygg0 8 9 the the DT cord-319626-f1b3ygg0 8 10 3H 3h CD cord-319626-f1b3ygg0 8 11 - - HYPH cord-319626-f1b3ygg0 8 12 labeled label VBN cord-319626-f1b3ygg0 8 13 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 8 14 bound bind VBN cord-319626-f1b3ygg0 8 15 to to IN cord-319626-f1b3ygg0 8 16 each each DT cord-319626-f1b3ygg0 8 17 of of IN cord-319626-f1b3ygg0 8 18 the the DT cord-319626-f1b3ygg0 8 19 E1 e1 NN cord-319626-f1b3ygg0 8 20 forms form NNS cord-319626-f1b3ygg0 8 21 using use VBG cord-319626-f1b3ygg0 8 22 gel gel NN cord-319626-f1b3ygg0 8 23 filtration filtration NN cord-319626-f1b3ygg0 8 24 on on IN cord-319626-f1b3ygg0 8 25 P4 P4 NNP cord-319626-f1b3ygg0 8 26 columns column NNS cord-319626-f1b3ygg0 8 27 . . . cord-319626-f1b3ygg0 9 1 The the DT cord-319626-f1b3ygg0 9 2 intracellular intracellular JJ cord-319626-f1b3ygg0 9 3 location location NN cord-319626-f1b3ygg0 9 4 of of IN cord-319626-f1b3ygg0 9 5 the the DT cord-319626-f1b3ygg0 9 6 first first JJ cord-319626-f1b3ygg0 9 7 step step NN cord-319626-f1b3ygg0 9 8 was be VBD cord-319626-f1b3ygg0 9 9 determined determine VBN cord-319626-f1b3ygg0 9 10 by by IN cord-319626-f1b3ygg0 9 11 exploiting exploit VBG cord-319626-f1b3ygg0 9 12 the the DT cord-319626-f1b3ygg0 9 13 temperature temperature NN cord-319626-f1b3ygg0 9 14 sensitivity sensitivity NN cord-319626-f1b3ygg0 9 15 of of IN cord-319626-f1b3ygg0 9 16 virus virus NN cord-319626-f1b3ygg0 9 17 release release NN cord-319626-f1b3ygg0 9 18 . . . cord-319626-f1b3ygg0 10 1 The the DT cord-319626-f1b3ygg0 10 2 virus virus NN cord-319626-f1b3ygg0 10 3 normally normally RB cord-319626-f1b3ygg0 10 4 buds bud VBZ cord-319626-f1b3ygg0 10 5 first first RB cord-319626-f1b3ygg0 10 6 into into IN cord-319626-f1b3ygg0 10 7 a a DT cord-319626-f1b3ygg0 10 8 smooth smooth JJ cord-319626-f1b3ygg0 10 9 membrane membrane NN cord-319626-f1b3ygg0 10 10 compartment compartment NN cord-319626-f1b3ygg0 10 11 lying lie VBG cord-319626-f1b3ygg0 10 12 between between IN cord-319626-f1b3ygg0 10 13 the the DT cord-319626-f1b3ygg0 10 14 rough rough JJ cord-319626-f1b3ygg0 10 15 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 10 16 reticulum reticulum NN cord-319626-f1b3ygg0 10 17 and and CC cord-319626-f1b3ygg0 10 18 the the DT cord-319626-f1b3ygg0 10 19 cis cis NNP cord-319626-f1b3ygg0 10 20 side side NN cord-319626-f1b3ygg0 10 21 of of IN cord-319626-f1b3ygg0 10 22 the the DT cord-319626-f1b3ygg0 10 23 Golgi Golgi NNP cord-319626-f1b3ygg0 10 24 stack stack NN cord-319626-f1b3ygg0 11 1 ( ( -LRB- cord-319626-f1b3ygg0 11 2 Tooze Tooze NNP cord-319626-f1b3ygg0 11 3 et et NNP cord-319626-f1b3ygg0 11 4 al al NNP cord-319626-f1b3ygg0 11 5 . . NNP cord-319626-f1b3ygg0 11 6 , , , cord-319626-f1b3ygg0 11 7 1984 1984 CD cord-319626-f1b3ygg0 11 8 ) ) -RRB- cord-319626-f1b3ygg0 11 9 . . . cord-319626-f1b3ygg0 12 1 At at IN cord-319626-f1b3ygg0 12 2 31 31 CD cord-319626-f1b3ygg0 12 3 degrees degree NNS cord-319626-f1b3ygg0 12 4 C c NN cord-319626-f1b3ygg0 12 5 the the DT cord-319626-f1b3ygg0 12 6 virus virus NN cord-319626-f1b3ygg0 12 7 is be VBZ cord-319626-f1b3ygg0 12 8 assembled assemble VBN cord-319626-f1b3ygg0 12 9 but but CC cord-319626-f1b3ygg0 12 10 does do VBZ cord-319626-f1b3ygg0 12 11 not not RB cord-319626-f1b3ygg0 12 12 appear appear VB cord-319626-f1b3ygg0 12 13 to to TO cord-319626-f1b3ygg0 12 14 enter enter VB cord-319626-f1b3ygg0 12 15 the the DT cord-319626-f1b3ygg0 12 16 Golgi Golgi NNP cord-319626-f1b3ygg0 12 17 stacks stack NNS cord-319626-f1b3ygg0 12 18 . . . cord-319626-f1b3ygg0 13 1 The the DT cord-319626-f1b3ygg0 13 2 addition addition NN cord-319626-f1b3ygg0 13 3 of of IN cord-319626-f1b3ygg0 13 4 N N NNP cord-319626-f1b3ygg0 13 5 - - HYPH cord-319626-f1b3ygg0 13 6 acetyl- acetyl- NN cord-319626-f1b3ygg0 13 7 galactosamine galactosamine NN cord-319626-f1b3ygg0 13 8 is be VBZ cord-319626-f1b3ygg0 13 9 unaffected unaffected JJ cord-319626-f1b3ygg0 13 10 although although IN cord-319626-f1b3ygg0 13 11 the the DT cord-319626-f1b3ygg0 13 12 addition addition NN cord-319626-f1b3ygg0 13 13 of of IN cord-319626-f1b3ygg0 13 14 galactose galactose NN cord-319626-f1b3ygg0 13 15 and and CC cord-319626-f1b3ygg0 13 16 sialic sialic JJ cord-319626-f1b3ygg0 13 17 acid acid NN cord-319626-f1b3ygg0 13 18 is be VBZ cord-319626-f1b3ygg0 13 19 inhibited inhibit VBN cord-319626-f1b3ygg0 13 20 . . . cord-319626-f1b3ygg0 14 1 These these DT cord-319626-f1b3ygg0 14 2 results result NNS cord-319626-f1b3ygg0 14 3 strongly strongly RB cord-319626-f1b3ygg0 14 4 suggest suggest VBP cord-319626-f1b3ygg0 14 5 that that IN cord-319626-f1b3ygg0 14 6 addition addition NN cord-319626-f1b3ygg0 14 7 of of IN cord-319626-f1b3ygg0 14 8 N n NN cord-319626-f1b3ygg0 14 9 - - HYPH cord-319626-f1b3ygg0 14 10 acetyl acetyl NN cord-319626-f1b3ygg0 14 11 - - HYPH cord-319626-f1b3ygg0 14 12 galactosamine galactosamine NN cord-319626-f1b3ygg0 14 13 occurs occur VBZ cord-319626-f1b3ygg0 14 14 in in IN cord-319626-f1b3ygg0 14 15 this this DT cord-319626-f1b3ygg0 14 16 budding bud VBG cord-319626-f1b3ygg0 14 17 compartment compartment NN cord-319626-f1b3ygg0 14 18 , , , cord-319626-f1b3ygg0 14 19 the the DT cord-319626-f1b3ygg0 14 20 morphology morphology NN cord-319626-f1b3ygg0 14 21 of of IN cord-319626-f1b3ygg0 14 22 which which WDT cord-319626-f1b3ygg0 14 23 is be VBZ cord-319626-f1b3ygg0 14 24 similar similar JJ cord-319626-f1b3ygg0 14 25 to to IN cord-319626-f1b3ygg0 14 26 that that DT cord-319626-f1b3ygg0 14 27 of of IN cord-319626-f1b3ygg0 14 28 transitional transitional JJ cord-319626-f1b3ygg0 14 29 elements element NNS cord-319626-f1b3ygg0 14 30 and and CC cord-319626-f1b3ygg0 14 31 vesicles vesicle NNS cord-319626-f1b3ygg0 14 32 . . . cord-319626-f1b3ygg0 15 1 ploiting ploite VBG cord-319626-f1b3ygg0 15 2 the the DT cord-319626-f1b3ygg0 15 3 temperature temperature NN cord-319626-f1b3ygg0 15 4 sensitivity sensitivity NN cord-319626-f1b3ygg0 15 5 of of IN cord-319626-f1b3ygg0 15 6 virus virus NN cord-319626-f1b3ygg0 15 7 release release NN cord-319626-f1b3ygg0 15 8 . . . cord-319626-f1b3ygg0 16 1 The the DT cord-319626-f1b3ygg0 16 2 virus virus NN cord-319626-f1b3ygg0 16 3 normally normally RB cord-319626-f1b3ygg0 16 4 buds bud VBZ cord-319626-f1b3ygg0 16 5 first first RB cord-319626-f1b3ygg0 16 6 into into IN cord-319626-f1b3ygg0 16 7 a a DT cord-319626-f1b3ygg0 16 8 smooth smooth JJ cord-319626-f1b3ygg0 16 9 membrane membrane NN cord-319626-f1b3ygg0 16 10 compartment compartment NN cord-319626-f1b3ygg0 16 11 lying lie VBG cord-319626-f1b3ygg0 16 12 between between IN cord-319626-f1b3ygg0 16 13 the the DT cord-319626-f1b3ygg0 16 14 rough rough JJ cord-319626-f1b3ygg0 16 15 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 16 16 reticulum reticulum NN cord-319626-f1b3ygg0 16 17 and and CC cord-319626-f1b3ygg0 16 18 the the DT cord-319626-f1b3ygg0 16 19 cis cis NNP cord-319626-f1b3ygg0 16 20 side side NN cord-319626-f1b3ygg0 16 21 of of IN cord-319626-f1b3ygg0 16 22 the the DT cord-319626-f1b3ygg0 16 23 Golgi Golgi NNP cord-319626-f1b3ygg0 16 24 stack stack NN cord-319626-f1b3ygg0 17 1 ( ( -LRB- cord-319626-f1b3ygg0 17 2 Tooze Tooze NNP cord-319626-f1b3ygg0 17 3 et et NNP cord-319626-f1b3ygg0 17 4 al al NNP cord-319626-f1b3ygg0 17 5 . . NNP cord-319626-f1b3ygg0 17 6 , , , cord-319626-f1b3ygg0 17 7 1984 1984 CD cord-319626-f1b3ygg0 17 8 ) ) -RRB- cord-319626-f1b3ygg0 17 9 . . . cord-319626-f1b3ygg0 18 1 At at IN cord-319626-f1b3ygg0 18 2 31 31 CD cord-319626-f1b3ygg0 18 3 ° ° NN cord-319626-f1b3ygg0 18 4 C C NNP cord-319626-f1b3ygg0 18 5 the the DT cord-319626-f1b3ygg0 18 6 virus virus NN cord-319626-f1b3ygg0 18 7 is be VBZ cord-319626-f1b3ygg0 18 8 assembled assemble VBN cord-319626-f1b3ygg0 18 9 but but CC cord-319626-f1b3ygg0 18 10 does do VBZ cord-319626-f1b3ygg0 18 11 not not RB cord-319626-f1b3ygg0 18 12 appear appear VB cord-319626-f1b3ygg0 18 13 to to TO cord-319626-f1b3ygg0 18 14 enter enter VB cord-319626-f1b3ygg0 18 15 the the DT cord-319626-f1b3ygg0 18 16 Golgi Golgi NNP cord-319626-f1b3ygg0 18 17 stacks stack NNS cord-319626-f1b3ygg0 18 18 . . . cord-319626-f1b3ygg0 19 1 The the DT cord-319626-f1b3ygg0 19 2 addition addition NN cord-319626-f1b3ygg0 19 3 of of IN cord-319626-f1b3ygg0 19 4 N n NN cord-319626-f1b3ygg0 19 5 - - HYPH cord-319626-f1b3ygg0 19 6 acetyl acetyl NN cord-319626-f1b3ygg0 19 7 - - HYPH cord-319626-f1b3ygg0 19 8 galactosamine galactosamine NNP cord-319626-f1b3ygg0 19 9 is be VBZ cord-319626-f1b3ygg0 19 10 unaffected unaffected JJ cord-319626-f1b3ygg0 19 11 although although IN cord-319626-f1b3ygg0 19 12 the the DT cord-319626-f1b3ygg0 19 13 addition addition NN cord-319626-f1b3ygg0 19 14 of of IN cord-319626-f1b3ygg0 19 15 galactose galactose NN cord-319626-f1b3ygg0 19 16 and and CC cord-319626-f1b3ygg0 19 17 sialic sialic JJ cord-319626-f1b3ygg0 19 18 acid acid NN cord-319626-f1b3ygg0 19 19 is be VBZ cord-319626-f1b3ygg0 19 20 inhibited inhibit VBN cord-319626-f1b3ygg0 19 21 . . . cord-319626-f1b3ygg0 20 1 These these DT cord-319626-f1b3ygg0 20 2 results result NNS cord-319626-f1b3ygg0 20 3 strongly strongly RB cord-319626-f1b3ygg0 20 4 suggest suggest VBP cord-319626-f1b3ygg0 20 5 that that IN cord-319626-f1b3ygg0 20 6 addition addition NN cord-319626-f1b3ygg0 20 7 of of IN cord-319626-f1b3ygg0 20 8 N n NN cord-319626-f1b3ygg0 20 9 - - HYPH cord-319626-f1b3ygg0 20 10 acetyl acetyl NN cord-319626-f1b3ygg0 20 11 - - HYPH cord-319626-f1b3ygg0 20 12 galactosamine galactosamine NN cord-319626-f1b3ygg0 20 13 occurs occur VBZ cord-319626-f1b3ygg0 20 14 in in IN cord-319626-f1b3ygg0 20 15 this this DT cord-319626-f1b3ygg0 20 16 budding bud VBG cord-319626-f1b3ygg0 20 17 compartment compartment NN cord-319626-f1b3ygg0 20 18 , , , cord-319626-f1b3ygg0 20 19 the the DT cord-319626-f1b3ygg0 20 20 morphology morphology NN cord-319626-f1b3ygg0 20 21 of of IN cord-319626-f1b3ygg0 20 22 which which WDT cord-319626-f1b3ygg0 20 23 is be VBZ cord-319626-f1b3ygg0 20 24 similar similar JJ cord-319626-f1b3ygg0 20 25 to to IN cord-319626-f1b3ygg0 20 26 that that DT cord-319626-f1b3ygg0 20 27 of of IN cord-319626-f1b3ygg0 20 28 transitional transitional JJ cord-319626-f1b3ygg0 20 29 elements element NNS cord-319626-f1b3ygg0 20 30 and and CC cord-319626-f1b3ygg0 20 31 vesicles vesicle NNS cord-319626-f1b3ygg0 20 32 . . . cord-319626-f1b3ygg0 21 1 p p LS cord-319626-f1b3ygg0 21 2 ROTEINS ROTEINS NNP cord-319626-f1b3ygg0 21 3 exported export VBN cord-319626-f1b3ygg0 21 4 from from IN cord-319626-f1b3ygg0 21 5 the the DT cord-319626-f1b3ygg0 21 6 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 21 7 reticulum reticulum NN cord-319626-f1b3ygg0 21 8 ( ( -LRB- cord-319626-f1b3ygg0 21 9 ER ER NNP cord-319626-f1b3ygg0 21 10 ) ) -RRB- cord-319626-f1b3ygg0 21 11 ' ' '' cord-319626-f1b3ygg0 21 12 move move NN cord-319626-f1b3ygg0 21 13 through through IN cord-319626-f1b3ygg0 21 14 each each DT cord-319626-f1b3ygg0 21 15 of of IN cord-319626-f1b3ygg0 21 16 the the DT cord-319626-f1b3ygg0 21 17 cisternae cisternae NN cord-319626-f1b3ygg0 21 18 of of IN cord-319626-f1b3ygg0 21 19 the the DT cord-319626-f1b3ygg0 21 20 Golgi Golgi NNP cord-319626-f1b3ygg0 21 21 stack stack NN cord-319626-f1b3ygg0 21 22 before before IN cord-319626-f1b3ygg0 21 23 they -PRON- PRP cord-319626-f1b3ygg0 21 24 are be VBP cord-319626-f1b3ygg0 21 25 directed direct VBN cord-319626-f1b3ygg0 21 26 to to IN cord-319626-f1b3ygg0 21 27 their -PRON- PRP$ cord-319626-f1b3ygg0 21 28 correct correct JJ cord-319626-f1b3ygg0 21 29 destination destination NN cord-319626-f1b3ygg0 21 30 either either CC cord-319626-f1b3ygg0 21 31 inside inside IN cord-319626-f1b3ygg0 21 32 or or CC cord-319626-f1b3ygg0 21 33 outside outside IN cord-319626-f1b3ygg0 21 34 the the DT cord-319626-f1b3ygg0 21 35 cell cell NN cord-319626-f1b3ygg0 21 36 ( ( -LRB- cord-319626-f1b3ygg0 21 37 Rothman Rothman NNP cord-319626-f1b3ygg0 21 38 , , , cord-319626-f1b3ygg0 21 39 1981 1981 CD cord-319626-f1b3ygg0 21 40 ; ; . cord-319626-f1b3ygg0 22 1 Griffiths Griffiths NNP cord-319626-f1b3ygg0 22 2 and and CC cord-319626-f1b3ygg0 22 3 Simons Simons NNP cord-319626-f1b3ygg0 22 4 , , , cord-319626-f1b3ygg0 22 5 1986 1986 CD cord-319626-f1b3ygg0 22 6 ; ; : cord-319626-f1b3ygg0 22 7 Pfeffer Pfeffer NNP cord-319626-f1b3ygg0 22 8 and and CC cord-319626-f1b3ygg0 22 9 Rothman Rothman NNP cord-319626-f1b3ygg0 22 10 , , , cord-319626-f1b3ygg0 22 11 1987 1987 CD cord-319626-f1b3ygg0 22 12 ) ) -RRB- cord-319626-f1b3ygg0 22 13 . . . cord-319626-f1b3ygg0 23 1 Many many JJ cord-319626-f1b3ygg0 23 2 proteins protein NNS cord-319626-f1b3ygg0 23 3 undergo undergo VBP cord-319626-f1b3ygg0 23 4 a a DT cord-319626-f1b3ygg0 23 5 series series NN cord-319626-f1b3ygg0 23 6 of of IN cord-319626-f1b3ygg0 23 7 posttranslational posttranslational JJ cord-319626-f1b3ygg0 23 8 modifications modification NNS cord-319626-f1b3ygg0 23 9 during during IN cord-319626-f1b3ygg0 23 10 this this DT cord-319626-f1b3ygg0 23 11 transport transport NN cord-319626-f1b3ygg0 23 12 including include VBG cord-319626-f1b3ygg0 23 13 the the DT cord-319626-f1b3ygg0 23 14 addition addition NN cord-319626-f1b3ygg0 23 15 of of IN cord-319626-f1b3ygg0 23 16 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 23 17 which which WDT cord-319626-f1b3ygg0 23 18 are be VBP cord-319626-f1b3ygg0 23 19 either either CC cord-319626-f1b3ygg0 23 20 N n NN cord-319626-f1b3ygg0 23 21 - - HYPH cord-319626-f1b3ygg0 23 22 linked link VBN cord-319626-f1b3ygg0 23 23 to to IN cord-319626-f1b3ygg0 23 24 asparagine asparagine NN cord-319626-f1b3ygg0 23 25 or or CC cord-319626-f1b3ygg0 23 26 O o NN cord-319626-f1b3ygg0 23 27 - - HYPH cord-319626-f1b3ygg0 23 28 linked link VBN cord-319626-f1b3ygg0 23 29 to to IN cord-319626-f1b3ygg0 23 30 serine serine NN cord-319626-f1b3ygg0 23 31 or or CC cord-319626-f1b3ygg0 23 32 threonine threonine NN cord-319626-f1b3ygg0 23 33 . . . cord-319626-f1b3ygg0 24 1 The the DT cord-319626-f1b3ygg0 24 2 sequence sequence NN cord-319626-f1b3ygg0 24 3 of of IN cord-319626-f1b3ygg0 24 4 modifications modification NNS cord-319626-f1b3ygg0 24 5 to to IN cord-319626-f1b3ygg0 24 6 these these DT cord-319626-f1b3ygg0 24 7 covalently covalently RB cord-319626-f1b3ygg0 24 8 bound bind VBN cord-319626-f1b3ygg0 24 9 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 24 10 reflects reflect VBZ cord-319626-f1b3ygg0 24 11 the the DT cord-319626-f1b3ygg0 24 12 movement movement NN cord-319626-f1b3ygg0 24 13 of of IN cord-319626-f1b3ygg0 24 14 the the DT cord-319626-f1b3ygg0 24 15 protein protein NN cord-319626-f1b3ygg0 24 16 from from IN cord-319626-f1b3ygg0 24 17 one one CD cord-319626-f1b3ygg0 24 18 compartment compartment NN cord-319626-f1b3ygg0 24 19 to to IN cord-319626-f1b3ygg0 24 20 the the DT cord-319626-f1b3ygg0 24 21 next next JJ cord-319626-f1b3ygg0 24 22 on on IN cord-319626-f1b3ygg0 24 23 the the DT cord-319626-f1b3ygg0 24 24 transport transport NN cord-319626-f1b3ygg0 24 25 pathway pathway NN cord-319626-f1b3ygg0 24 26 . . . cord-319626-f1b3ygg0 25 1 The the DT cord-319626-f1b3ygg0 25 2 addition addition NN cord-319626-f1b3ygg0 25 3 and and CC cord-319626-f1b3ygg0 25 4 modification modification NN cord-319626-f1b3ygg0 25 5 of of IN cord-319626-f1b3ygg0 25 6 the the DT cord-319626-f1b3ygg0 25 7 N n NN cord-319626-f1b3ygg0 25 8 - - HYPH cord-319626-f1b3ygg0 25 9 linked link VBN cord-319626-f1b3ygg0 25 10 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 25 11 has have VBZ cord-319626-f1b3ygg0 25 12 been be VBN cord-319626-f1b3ygg0 25 13 extensively extensively RB cord-319626-f1b3ygg0 25 14 investigated investigate VBN cord-319626-f1b3ygg0 25 15 ( ( -LRB- cord-319626-f1b3ygg0 25 16 reviewed review VBN cord-319626-f1b3ygg0 25 17 by by IN cord-319626-f1b3ygg0 25 18 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 25 19 and and CC cord-319626-f1b3ygg0 25 20 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 25 21 , , , cord-319626-f1b3ygg0 25 22 1980 1980 CD cord-319626-f1b3ygg0 25 23 ) ) -RRB- cord-319626-f1b3ygg0 25 24 but but CC cord-319626-f1b3ygg0 25 25 much much RB cord-319626-f1b3ygg0 25 26 less less JJR cord-319626-f1b3ygg0 25 27 is be VBZ cord-319626-f1b3ygg0 25 28 known know VBN cord-319626-f1b3ygg0 25 29 about about IN cord-319626-f1b3ygg0 25 30 the the DT cord-319626-f1b3ygg0 25 31 addition addition NN cord-319626-f1b3ygg0 25 32 of of IN cord-319626-f1b3ygg0 25 33 O o NN cord-319626-f1b3ygg0 25 34 - - HYPH cord-319626-f1b3ygg0 25 35 linked link VBN cord-319626-f1b3ygg0 25 36 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 25 37 . . . cord-319626-f1b3ygg0 26 1 For for IN cord-319626-f1b3ygg0 26 2 the the DT cord-319626-f1b3ygg0 26 3 simplest simple JJS cord-319626-f1b3ygg0 26 4 and and CC cord-319626-f1b3ygg0 26 5 most most RBS cord-319626-f1b3ygg0 26 6 common common JJ cord-319626-f1b3ygg0 26 7 type type NN cord-319626-f1b3ygg0 26 8 there there EX cord-319626-f1b3ygg0 26 9 is be VBZ cord-319626-f1b3ygg0 26 10 indirect indirect JJ cord-319626-f1b3ygg0 26 11 evidence evidence NN cord-319626-f1b3ygg0 26 12 that that IN cord-319626-f1b3ygg0 26 13 construction construction NN cord-319626-f1b3ygg0 26 14 of of IN cord-319626-f1b3ygg0 26 15 the the DT cord-319626-f1b3ygg0 26 16 O o NN cord-319626-f1b3ygg0 26 17 - - HYPH cord-319626-f1b3ygg0 26 18 linked link VBN cord-319626-f1b3ygg0 26 19 glycans glycan NNS cord-319626-f1b3ygg0 26 20 is be VBZ cord-319626-f1b3ygg0 26 21 a a DT cord-319626-f1b3ygg0 26 22 two two CD cord-319626-f1b3ygg0 26 23 - - HYPH cord-319626-f1b3ygg0 26 24 step step NN cord-319626-f1b3ygg0 26 25 process process NN cord-319626-f1b3ygg0 26 26 , , , cord-319626-f1b3ygg0 26 27 the the DT cord-319626-f1b3ygg0 26 28 addition addition NN cord-319626-f1b3ygg0 26 29 of of IN cord-319626-f1b3ygg0 26 30 N n NN cord-319626-f1b3ygg0 26 31 - - HYPH cord-319626-f1b3ygg0 26 32 acetyl acetyl NN cord-319626-f1b3ygg0 26 33 - - HYPH cord-319626-f1b3ygg0 26 34 galactosamine galactosamine NNP cord-319626-f1b3ygg0 26 35 ( ( -LRB- cord-319626-f1b3ygg0 26 36 GalNAc GalNAc NNP cord-319626-f1b3ygg0 26 37 ) ) -RRB- cord-319626-f1b3ygg0 26 38 preceding precede VBG cord-319626-f1b3ygg0 26 39 that that DT cord-319626-f1b3ygg0 26 40 of of IN cord-319626-f1b3ygg0 26 41 galactose galactose NN cord-319626-f1b3ygg0 26 42 ( ( -LRB- cord-319626-f1b3ygg0 26 43 Gal Gal NNP cord-319626-f1b3ygg0 26 44 ) ) -RRB- cord-319626-f1b3ygg0 26 45 and and CC cord-319626-f1b3ygg0 26 46 sialic sialic JJ cord-319626-f1b3ygg0 26 47 acid acid NN cord-319626-f1b3ygg0 26 48 ( ( -LRB- cord-319626-f1b3ygg0 26 49 NANA NANA NNP cord-319626-f1b3ygg0 26 50 ) ) -RRB- cord-319626-f1b3ygg0 26 51 . . . cord-319626-f1b3ygg0 27 1 The the DT cord-319626-f1b3ygg0 27 2 addition addition NN cord-319626-f1b3ygg0 27 3 of of IN cord-319626-f1b3ygg0 27 4 the the DT cord-319626-f1b3ygg0 27 5 last last JJ cord-319626-f1b3ygg0 27 6 two two CD cord-319626-f1b3ygg0 27 7 sugars sugar NNS cord-319626-f1b3ygg0 27 8 almost almost RB cord-319626-f1b3ygg0 27 9 certainly certainly RB cord-319626-f1b3ygg0 27 10 occurs occur VBZ cord-319626-f1b3ygg0 27 11 in in IN cord-319626-f1b3ygg0 27 12 the the DT cord-319626-f1b3ygg0 27 13 trans trans JJ cord-319626-f1b3ygg0 27 14 part part NN cord-319626-f1b3ygg0 27 15 of of IN cord-319626-f1b3ygg0 27 16 the the DT cord-319626-f1b3ygg0 27 17 Golgi Golgi NNP cord-319626-f1b3ygg0 27 18 stack stack NN cord-319626-f1b3ygg0 28 1 ( ( -LRB- cord-319626-f1b3ygg0 28 2 Cummings Cummings NNP cord-319626-f1b3ygg0 28 3 et et FW cord-319626-f1b3ygg0 28 4 al al NNP cord-319626-f1b3ygg0 28 5 . . NNP cord-319626-f1b3ygg0 28 6 , , , cord-319626-f1b3ygg0 28 7 1983 1983 CD cord-319626-f1b3ygg0 28 8 ; ; : cord-319626-f1b3ygg0 28 9 Elhammer Elhammer NNP cord-319626-f1b3ygg0 28 10 and and CC cord-319626-f1b3ygg0 28 11 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 28 12 , , , cord-319626-f1b3ygg0 28 13 1984 1984 CD cord-319626-f1b3ygg0 28 14 ) ) -RRB- cord-319626-f1b3ygg0 28 15 . . . cord-319626-f1b3ygg0 29 1 Proposals proposal NNS cord-319626-f1b3ygg0 29 2 for for IN cord-319626-f1b3ygg0 29 3 the the DT cord-319626-f1b3ygg0 29 4 site site NN cord-319626-f1b3ygg0 29 5 of of IN cord-319626-f1b3ygg0 29 6 addition addition NN cord-319626-f1b3ygg0 29 7 of of IN cord-319626-f1b3ygg0 29 8 the the DT cord-319626-f1b3ygg0 29 9 first first JJ cord-319626-f1b3ygg0 29 10 sugar sugar NN cord-319626-f1b3ygg0 29 11 , , , cord-319626-f1b3ygg0 29 12 however however RB cord-319626-f1b3ygg0 29 13 , , , cord-319626-f1b3ygg0 29 14 range range VBP cord-319626-f1b3ygg0 29 15 from from IN cord-319626-f1b3ygg0 29 16 the the DT cord-319626-f1b3ygg0 29 17 rough rough JJ cord-319626-f1b3ygg0 29 18 ER er NN cord-319626-f1b3ygg0 29 19 ( ( -LRB- cord-319626-f1b3ygg0 29 20 Strous Strous NNP cord-319626-f1b3ygg0 29 21 , , , cord-319626-f1b3ygg0 29 22 1979 1979 CD cord-319626-f1b3ygg0 29 23 ) ) -RRB- cord-319626-f1b3ygg0 29 24 , , , cord-319626-f1b3ygg0 29 25 where where WRB cord-319626-f1b3ygg0 29 26 the the DT cord-319626-f1b3ygg0 29 27 proteins protein NNS cord-319626-f1b3ygg0 29 28 are be VBP cord-319626-f1b3ygg0 29 29 assembled assemble VBN cord-319626-f1b3ygg0 29 30 , , , cord-319626-f1b3ygg0 29 31 to to IN cord-319626-f1b3ygg0 29 32 the the DT cord-319626-f1b3ygg0 29 33 1 1 CD cord-319626-f1b3ygg0 29 34 . . . cord-319626-f1b3ygg0 30 1 Abbreviations abbreviation NNS cord-319626-f1b3ygg0 30 2 used use VBN cord-319626-f1b3ygg0 30 3 in in IN cord-319626-f1b3ygg0 30 4 this this DT cord-319626-f1b3ygg0 30 5 paper paper NN cord-319626-f1b3ygg0 30 6 : : : cord-319626-f1b3ygg0 30 7 ER ER NNP cord-319626-f1b3ygg0 30 8 , , , cord-319626-f1b3ygg0 30 9 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 30 10 reticulum reticulum NN cord-319626-f1b3ygg0 30 11 ; ; : cord-319626-f1b3ygg0 30 12 Gal gal NN cord-319626-f1b3ygg0 30 13 , , , cord-319626-f1b3ygg0 30 14 galactose galactose NN cord-319626-f1b3ygg0 30 15 ; ; : cord-319626-f1b3ygg0 30 16 GalNAc GalNAc NNP cord-319626-f1b3ygg0 30 17 , , , cord-319626-f1b3ygg0 30 18 N N NNP cord-319626-f1b3ygg0 30 19 - - HYPH cord-319626-f1b3ygg0 30 20 acetyl acetyl NN cord-319626-f1b3ygg0 30 21 - - HYPH cord-319626-f1b3ygg0 30 22 galactosamine galactosamine NN cord-319626-f1b3ygg0 30 23 ; ; : cord-319626-f1b3ygg0 30 24 MEM mem NN cord-319626-f1b3ygg0 30 25 - - HYPH cord-319626-f1b3ygg0 30 26 met meet VBN cord-319626-f1b3ygg0 30 27 , , , cord-319626-f1b3ygg0 30 28 methionine methionine NN cord-319626-f1b3ygg0 30 29 - - HYPH cord-319626-f1b3ygg0 30 30 free free JJ cord-319626-f1b3ygg0 30 31 modified modify VBN cord-319626-f1b3ygg0 30 32 Eagle Eagle NNP cord-319626-f1b3ygg0 30 33 's 's POS cord-319626-f1b3ygg0 30 34 medium medium NN cord-319626-f1b3ygg0 30 35 ; ; : cord-319626-f1b3ygg0 30 36 MHV MHV NNP cord-319626-f1b3ygg0 30 37 - - HYPH cord-319626-f1b3ygg0 30 38 A59 A59 NNP cord-319626-f1b3ygg0 30 39 , , , cord-319626-f1b3ygg0 30 40 mouse mouse NN cord-319626-f1b3ygg0 30 41 hepatitis hepatitis NN cord-319626-f1b3ygg0 30 42 virus virus NN cord-319626-f1b3ygg0 30 43 , , , cord-319626-f1b3ygg0 30 44 strain strain VB cord-319626-f1b3ygg0 30 45 A59 A59 NNP cord-319626-f1b3ygg0 30 46 ; ; : cord-319626-f1b3ygg0 30 47 NANA NANA NNP cord-319626-f1b3ygg0 30 48 , , , cord-319626-f1b3ygg0 30 49 N n CD cord-319626-f1b3ygg0 30 50 - - HYPH cord-319626-f1b3ygg0 30 51 acetyl acetyl NN cord-319626-f1b3ygg0 30 52 - - HYPH cord-319626-f1b3ygg0 30 53 neuraminic neuraminic JJ cord-319626-f1b3ygg0 30 54 acid acid NN cord-319626-f1b3ygg0 30 55 ( ( -LRB- cord-319626-f1b3ygg0 30 56 sialic sialic JJ cord-319626-f1b3ygg0 30 57 acid acid NN cord-319626-f1b3ygg0 30 58 ) ) -RRB- cord-319626-f1b3ygg0 30 59 ; ; : cord-319626-f1b3ygg0 31 1 Tx-114 Tx-114 NNP cord-319626-f1b3ygg0 31 2 , , , cord-319626-f1b3ygg0 31 3 Triton Triton NNP cord-319626-f1b3ygg0 31 4 X X NNP cord-319626-f1b3ygg0 31 5 - - HYPH cord-319626-f1b3ygg0 31 6 tI4 ti4 NN cord-319626-f1b3ygg0 31 7 . . . cord-319626-f1b3ygg0 32 1 cis cis NNP cord-319626-f1b3ygg0 32 2 part part NN cord-319626-f1b3ygg0 32 3 of of IN cord-319626-f1b3ygg0 32 4 the the DT cord-319626-f1b3ygg0 32 5 Golgi Golgi NNP cord-319626-f1b3ygg0 32 6 stack stack NN cord-319626-f1b3ygg0 32 7 which which WDT cord-319626-f1b3ygg0 32 8 is be VBZ cord-319626-f1b3ygg0 32 9 the the DT cord-319626-f1b3ygg0 32 10 entry entry NN cord-319626-f1b3ygg0 32 11 point point NN cord-319626-f1b3ygg0 32 12 for for IN cord-319626-f1b3ygg0 32 13 proteins protein NNS cord-319626-f1b3ygg0 32 14 that that WDT cord-319626-f1b3ygg0 32 15 have have VBP cord-319626-f1b3ygg0 32 16 left leave VBN cord-319626-f1b3ygg0 32 17 the the DT cord-319626-f1b3ygg0 32 18 ER er NN cord-319626-f1b3ygg0 33 1 ( ( -LRB- cord-319626-f1b3ygg0 33 2 Cummings Cummings NNP cord-319626-f1b3ygg0 33 3 et et FW cord-319626-f1b3ygg0 33 4 al al NNP cord-319626-f1b3ygg0 33 5 . . . cord-319626-f1b3ygg0 34 1 , , , cord-319626-f1b3ygg0 34 2 1983 1983 CD cord-319626-f1b3ygg0 34 3 ; ; : cord-319626-f1b3ygg0 34 4 Roth Roth NNP cord-319626-f1b3ygg0 34 5 , , , cord-319626-f1b3ygg0 34 6 1984 1984 CD cord-319626-f1b3ygg0 34 7 ) ) -RRB- cord-319626-f1b3ygg0 34 8 , , , cord-319626-f1b3ygg0 34 9 to to IN cord-319626-f1b3ygg0 34 10 the the DT cord-319626-f1b3ygg0 34 11 medial medial JJ cord-319626-f1b3ygg0 34 12 part part NN cord-319626-f1b3ygg0 35 1 ( ( -LRB- cord-319626-f1b3ygg0 35 2 Niemann Niemann NNP cord-319626-f1b3ygg0 35 3 et et NNP cord-319626-f1b3ygg0 35 4 al al NNP cord-319626-f1b3ygg0 35 5 . . NNP cord-319626-f1b3ygg0 35 6 , , , cord-319626-f1b3ygg0 35 7 1982 1982 CD cord-319626-f1b3ygg0 35 8 ; ; : cord-319626-f1b3ygg0 35 9 Elhammer Elhammer NNP cord-319626-f1b3ygg0 35 10 and and CC cord-319626-f1b3ygg0 35 11 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 35 12 , , , cord-319626-f1b3ygg0 35 13 1984 1984 CD cord-319626-f1b3ygg0 35 14 ) ) -RRB- cord-319626-f1b3ygg0 35 15 , , , cord-319626-f1b3ygg0 35 16 and and CC cord-319626-f1b3ygg0 35 17 finally finally RB cord-319626-f1b3ygg0 35 18 to to IN cord-319626-f1b3ygg0 35 19 the the DT cord-319626-f1b3ygg0 35 20 trans trans NNP cord-319626-f1b3ygg0 35 21 - - NNP cord-319626-f1b3ygg0 35 22 Golgi Golgi NNP cord-319626-f1b3ygg0 35 23 cisternae cisternae NNP cord-319626-f1b3ygg0 35 24 ( ( -LRB- cord-319626-f1b3ygg0 35 25 Johnson Johnson NNP cord-319626-f1b3ygg0 35 26 and and CC cord-319626-f1b3ygg0 35 27 Spear Spear NNP cord-319626-f1b3ygg0 35 28 , , , cord-319626-f1b3ygg0 35 29 1983 1983 CD cord-319626-f1b3ygg0 35 30 ) ) -RRB- cord-319626-f1b3ygg0 35 31 . . . cord-319626-f1b3ygg0 36 1 Most Most JJS cord-319626-f1b3ygg0 36 2 of of IN cord-319626-f1b3ygg0 36 3 the the DT cord-319626-f1b3ygg0 36 4 evidence evidence NN cord-319626-f1b3ygg0 36 5 supports support VBZ cord-319626-f1b3ygg0 36 6 the the DT cord-319626-f1b3ygg0 36 7 idea idea NN cord-319626-f1b3ygg0 36 8 that that IN cord-319626-f1b3ygg0 36 9 GalNAc GalNAc NNP cord-319626-f1b3ygg0 36 10 is be VBZ cord-319626-f1b3ygg0 36 11 added add VBN cord-319626-f1b3ygg0 36 12 either either CC cord-319626-f1b3ygg0 36 13 just just RB cord-319626-f1b3ygg0 36 14 before before RB cord-319626-f1b3ygg0 36 15 or or CC cord-319626-f1b3ygg0 36 16 just just RB cord-319626-f1b3ygg0 36 17 after after IN cord-319626-f1b3ygg0 36 18 the the DT cord-319626-f1b3ygg0 36 19 protein protein NN cord-319626-f1b3ygg0 36 20 leaves leave VBZ cord-319626-f1b3ygg0 36 21 the the DT cord-319626-f1b3ygg0 36 22 ER ER NNP cord-319626-f1b3ygg0 36 23 . . . cord-319626-f1b3ygg0 37 1 There there EX cord-319626-f1b3ygg0 37 2 are be VBP cord-319626-f1b3ygg0 37 3 several several JJ cord-319626-f1b3ygg0 37 4 reasons reason NNS cord-319626-f1b3ygg0 37 5 why why WRB cord-319626-f1b3ygg0 37 6 it -PRON- PRP cord-319626-f1b3ygg0 37 7 has have VBZ cord-319626-f1b3ygg0 37 8 proven prove VBN cord-319626-f1b3ygg0 37 9 so so RB cord-319626-f1b3ygg0 37 10 difficult difficult JJ cord-319626-f1b3ygg0 37 11 to to TO cord-319626-f1b3ygg0 37 12 locate locate VB cord-319626-f1b3ygg0 37 13 the the DT cord-319626-f1b3ygg0 37 14 site site NN cord-319626-f1b3ygg0 37 15 of of IN cord-319626-f1b3ygg0 37 16 this this DT cord-319626-f1b3ygg0 37 17 first first JJ cord-319626-f1b3ygg0 37 18 sugar sugar NN cord-319626-f1b3ygg0 37 19 addition addition NN cord-319626-f1b3ygg0 37 20 , , , cord-319626-f1b3ygg0 37 21 of of IN cord-319626-f1b3ygg0 37 22 which which WDT cord-319626-f1b3ygg0 37 23 the the DT cord-319626-f1b3ygg0 37 24 absence absence NN cord-319626-f1b3ygg0 37 25 of of IN cord-319626-f1b3ygg0 37 26 a a DT cord-319626-f1b3ygg0 37 27 combined combine VBN cord-319626-f1b3ygg0 37 28 biochemical biochemical JJ cord-319626-f1b3ygg0 37 29 and and CC cord-319626-f1b3ygg0 37 30 morphological morphological JJ cord-319626-f1b3ygg0 37 31 study study NN cord-319626-f1b3ygg0 37 32 of of IN cord-319626-f1b3ygg0 37 33 the the DT cord-319626-f1b3ygg0 37 34 O o NN cord-319626-f1b3ygg0 37 35 - - HYPH cord-319626-f1b3ygg0 37 36 glycosylation glycosylation NN cord-319626-f1b3ygg0 37 37 of of IN cord-319626-f1b3ygg0 37 38 a a DT cord-319626-f1b3ygg0 37 39 single single JJ cord-319626-f1b3ygg0 37 40 protein protein NN cord-319626-f1b3ygg0 37 41 must must MD cord-319626-f1b3ygg0 37 42 rank rank VB cord-319626-f1b3ygg0 37 43 as as IN cord-319626-f1b3ygg0 37 44 the the DT cord-319626-f1b3ygg0 37 45 most most RBS cord-319626-f1b3ygg0 37 46 important important JJ cord-319626-f1b3ygg0 37 47 . . . cord-319626-f1b3ygg0 38 1 The the DT cord-319626-f1b3ygg0 38 2 E1 e1 NN cord-319626-f1b3ygg0 38 3 envelope envelope NN cord-319626-f1b3ygg0 38 4 protein protein NN cord-319626-f1b3ygg0 38 5 of of IN cord-319626-f1b3ygg0 38 6 coronavirus coronavirus NN cord-319626-f1b3ygg0 38 7 MHV MHV NNP cord-319626-f1b3ygg0 38 8 - - HYPH cord-319626-f1b3ygg0 38 9 A59 A59 NNP cord-319626-f1b3ygg0 39 1 ( ( -LRB- cord-319626-f1b3ygg0 39 2 Mouse Mouse NNP cord-319626-f1b3ygg0 39 3 Hepatitis Hepatitis NNP cord-319626-f1b3ygg0 39 4 Virus Virus NNP cord-319626-f1b3ygg0 39 5 , , , cord-319626-f1b3ygg0 39 6 strain strain VB cord-319626-f1b3ygg0 39 7 A59 A59 NNP cord-319626-f1b3ygg0 39 8 ) ) -RRB- cord-319626-f1b3ygg0 39 9 contains contain VBZ cord-319626-f1b3ygg0 39 10 only only RB cord-319626-f1b3ygg0 39 11 O o NN cord-319626-f1b3ygg0 39 12 - - HYPH cord-319626-f1b3ygg0 39 13 linked link VBN cord-319626-f1b3ygg0 39 14 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 39 15 and and CC cord-319626-f1b3ygg0 39 16 is be VBZ cord-319626-f1b3ygg0 39 17 , , , cord-319626-f1b3ygg0 39 18 therefore therefore RB cord-319626-f1b3ygg0 39 19 , , , cord-319626-f1b3ygg0 39 20 a a DT cord-319626-f1b3ygg0 39 21 good good JJ cord-319626-f1b3ygg0 39 22 model model NN cord-319626-f1b3ygg0 39 23 for for IN cord-319626-f1b3ygg0 39 24 studying study VBG cord-319626-f1b3ygg0 39 25 addition addition NN cord-319626-f1b3ygg0 39 26 of of IN cord-319626-f1b3ygg0 39 27 O o NN cord-319626-f1b3ygg0 39 28 - - HYPH cord-319626-f1b3ygg0 39 29 linked link VBN cord-319626-f1b3ygg0 39 30 sugars sugar NNS cord-319626-f1b3ygg0 39 31 . . . cord-319626-f1b3ygg0 40 1 MHV MHV NNP cord-319626-f1b3ygg0 40 2 - - HYPH cord-319626-f1b3ygg0 40 3 A59 A59 NNP cord-319626-f1b3ygg0 40 4 buds bud NNS cord-319626-f1b3ygg0 40 5 intracellularly intracellularly RB cord-319626-f1b3ygg0 40 6 and and CC cord-319626-f1b3ygg0 40 7 E1 E1 NNP cord-319626-f1b3ygg0 40 8 is be VBZ cord-319626-f1b3ygg0 40 9 the the DT cord-319626-f1b3ygg0 40 10 only only JJ cord-319626-f1b3ygg0 40 11 viral viral JJ cord-319626-f1b3ygg0 40 12 membrane membrane NN cord-319626-f1b3ygg0 40 13 protein protein NN cord-319626-f1b3ygg0 40 14 needed need VBN cord-319626-f1b3ygg0 40 15 for for IN cord-319626-f1b3ygg0 40 16 budding bud VBG cord-319626-f1b3ygg0 40 17 ( ( -LRB- cord-319626-f1b3ygg0 40 18 Holmes Holmes NNP cord-319626-f1b3ygg0 40 19 et et NNP cord-319626-f1b3ygg0 40 20 al al NNP cord-319626-f1b3ygg0 40 21 . . NNP cord-319626-f1b3ygg0 40 22 , , , cord-319626-f1b3ygg0 40 23 1981a 1981a CD cord-319626-f1b3ygg0 40 24 , , , cord-319626-f1b3ygg0 40 25 b b NN cord-319626-f1b3ygg0 40 26 ; ; : cord-319626-f1b3ygg0 41 1 Rottier Rottier NNP cord-319626-f1b3ygg0 41 2 et et NNP cord-319626-f1b3ygg0 41 3 al al NNP cord-319626-f1b3ygg0 41 4 . . NNP cord-319626-f1b3ygg0 41 5 , , , cord-319626-f1b3ygg0 41 6 1981 1981 CD cord-319626-f1b3ygg0 41 7 ) ) -RRB- cord-319626-f1b3ygg0 41 8 . . . cord-319626-f1b3ygg0 42 1 After after IN cord-319626-f1b3ygg0 42 2 synthesis synthesis NN cord-319626-f1b3ygg0 42 3 in in IN cord-319626-f1b3ygg0 42 4 the the DT cord-319626-f1b3ygg0 42 5 rough rough JJ cord-319626-f1b3ygg0 42 6 ER er NN cord-319626-f1b3ygg0 42 7 this this DT cord-319626-f1b3ygg0 42 8 protein protein NN cord-319626-f1b3ygg0 42 9 is be VBZ cord-319626-f1b3ygg0 42 10 transported transport VBN cord-319626-f1b3ygg0 42 11 to to IN cord-319626-f1b3ygg0 42 12 a a DT cord-319626-f1b3ygg0 42 13 smooth smooth JJ cord-319626-f1b3ygg0 42 14 membrane membrane NN cord-319626-f1b3ygg0 42 15 compartment compartment NN cord-319626-f1b3ygg0 42 16 between between IN cord-319626-f1b3ygg0 42 17 the the DT cord-319626-f1b3ygg0 42 18 rough rough JJ cord-319626-f1b3ygg0 42 19 ER ER NNP cord-319626-f1b3ygg0 42 20 and and CC cord-319626-f1b3ygg0 42 21 Golgi Golgi NNP cord-319626-f1b3ygg0 42 22 stack stack NN cord-319626-f1b3ygg0 42 23 where where WRB cord-319626-f1b3ygg0 42 24 it -PRON- PRP cord-319626-f1b3ygg0 42 25 accumulates accumulate VBZ cord-319626-f1b3ygg0 42 26 allowing allow VBG cord-319626-f1b3ygg0 42 27 nucleocapsids nucleocapsid NNS cord-319626-f1b3ygg0 42 28 in in IN cord-319626-f1b3ygg0 42 29 the the DT cord-319626-f1b3ygg0 42 30 cytoplasm cytoplasm NN cord-319626-f1b3ygg0 42 31 to to TO cord-319626-f1b3ygg0 42 32 bind bind VB cord-319626-f1b3ygg0 42 33 which which WDT cord-319626-f1b3ygg0 42 34 results result NNS cord-319626-f1b3ygg0 42 35 in in IN cord-319626-f1b3ygg0 42 36 the the DT cord-319626-f1b3ygg0 42 37 formation formation NN cord-319626-f1b3ygg0 42 38 of of IN cord-319626-f1b3ygg0 42 39 a a DT cord-319626-f1b3ygg0 42 40 virion virion NN cord-319626-f1b3ygg0 42 41 ( ( -LRB- cord-319626-f1b3ygg0 43 1 Holmes Holmes NNP cord-319626-f1b3ygg0 43 2 et et NNP cord-319626-f1b3ygg0 43 3 al al NNP cord-319626-f1b3ygg0 43 4 . . . cord-319626-f1b3ygg0 44 1 , , , cord-319626-f1b3ygg0 44 2 1981a 1981a CD cord-319626-f1b3ygg0 44 3 ; ; : cord-319626-f1b3ygg0 44 4 Tooze Tooze NNP cord-319626-f1b3ygg0 44 5 et et NNP cord-319626-f1b3ygg0 44 6 al al NNP cord-319626-f1b3ygg0 44 7 . . NNP cord-319626-f1b3ygg0 44 8 , , , cord-319626-f1b3ygg0 44 9 1984 1984 CD cord-319626-f1b3ygg0 44 10 ) ) -RRB- cord-319626-f1b3ygg0 44 11 . . . cord-319626-f1b3ygg0 45 1 The the DT cord-319626-f1b3ygg0 45 2 virions virion NNS cord-319626-f1b3ygg0 45 3 then then RB cord-319626-f1b3ygg0 45 4 follow follow VBP cord-319626-f1b3ygg0 45 5 the the DT cord-319626-f1b3ygg0 45 6 secretory secretory JJ cord-319626-f1b3ygg0 45 7 pathway pathway NN cord-319626-f1b3ygg0 45 8 out out IN cord-319626-f1b3ygg0 45 9 of of IN cord-319626-f1b3ygg0 45 10 the the DT cord-319626-f1b3ygg0 45 11 cell cell NN cord-319626-f1b3ygg0 46 1 ( ( -LRB- cord-319626-f1b3ygg0 46 2 Holmes Holmes NNP cord-319626-f1b3ygg0 46 3 et et NNP cord-319626-f1b3ygg0 46 4 al al NNP cord-319626-f1b3ygg0 46 5 . . NNP cord-319626-f1b3ygg0 46 6 , , , cord-319626-f1b3ygg0 46 7 1981b 1981b CD cord-319626-f1b3ygg0 46 8 ; ; : cord-319626-f1b3ygg0 47 1 Tooze Tooze NNP cord-319626-f1b3ygg0 47 2 et et NNP cord-319626-f1b3ygg0 47 3 al al NNP cord-319626-f1b3ygg0 47 4 . . NNP cord-319626-f1b3ygg0 47 5 , , , cord-319626-f1b3ygg0 47 6 1987 1987 CD cord-319626-f1b3ygg0 47 7 ) ) -RRB- cord-319626-f1b3ygg0 47 8 . . . cord-319626-f1b3ygg0 48 1 The the DT cord-319626-f1b3ygg0 48 2 virions virion NNS cord-319626-f1b3ygg0 48 3 contain contain VBP cord-319626-f1b3ygg0 48 4 in in IN cord-319626-f1b3ygg0 48 5 addition addition NN cord-319626-f1b3ygg0 48 6 to to IN cord-319626-f1b3ygg0 48 7 E1 E1 NNP cord-319626-f1b3ygg0 48 8 a a DT cord-319626-f1b3ygg0 48 9 second second JJ cord-319626-f1b3ygg0 48 10 envelope envelope NN cord-319626-f1b3ygg0 48 11 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 48 12 , , , cord-319626-f1b3ygg0 48 13 E2 E2 NNP cord-319626-f1b3ygg0 48 14 , , , cord-319626-f1b3ygg0 48 15 which which WDT cord-319626-f1b3ygg0 48 16 is be VBZ cord-319626-f1b3ygg0 48 17 essential essential JJ cord-319626-f1b3ygg0 48 18 for for IN cord-319626-f1b3ygg0 48 19 infectivity infectivity NN cord-319626-f1b3ygg0 48 20 ( ( -LRB- cord-319626-f1b3ygg0 48 21 Sturman Sturman NNP cord-319626-f1b3ygg0 48 22 , , , cord-319626-f1b3ygg0 48 23 1981 1981 CD cord-319626-f1b3ygg0 48 24 ) ) -RRB- cord-319626-f1b3ygg0 48 25 and and CC cord-319626-f1b3ygg0 48 26 is be VBZ cord-319626-f1b3ygg0 48 27 incorporated incorporate VBN cord-319626-f1b3ygg0 48 28 at at IN cord-319626-f1b3ygg0 48 29 some some DT cord-319626-f1b3ygg0 48 30 as as IN cord-319626-f1b3ygg0 48 31 yet yet RB cord-319626-f1b3ygg0 48 32 undefined undefined JJ cord-319626-f1b3ygg0 48 33 stage stage NN cord-319626-f1b3ygg0 48 34 during during IN cord-319626-f1b3ygg0 48 35 the the DT cord-319626-f1b3ygg0 48 36 budding bud VBG cord-319626-f1b3ygg0 48 37 or or CC cord-319626-f1b3ygg0 48 38 transport transport NN cord-319626-f1b3ygg0 48 39 of of IN cord-319626-f1b3ygg0 48 40 the the DT cord-319626-f1b3ygg0 48 41 virions virion NNS cord-319626-f1b3ygg0 48 42 through through IN cord-319626-f1b3ygg0 48 43 intracellular intracellular JJ cord-319626-f1b3ygg0 48 44 compartments compartment NNS cord-319626-f1b3ygg0 48 45 . . . cord-319626-f1b3ygg0 49 1 E2 E2 NNP cord-319626-f1b3ygg0 49 2 is be VBZ cord-319626-f1b3ygg0 49 3 a a DT cord-319626-f1b3ygg0 49 4 fusogen fusogen NN cord-319626-f1b3ygg0 49 5 and and CC cord-319626-f1b3ygg0 49 6 is be VBZ cord-319626-f1b3ygg0 49 7 also also RB cord-319626-f1b3ygg0 49 8 transported transport VBN cord-319626-f1b3ygg0 49 9 in in IN cord-319626-f1b3ygg0 49 10 cellular cellular JJ cord-319626-f1b3ygg0 49 11 membranes membrane NNS cord-319626-f1b3ygg0 49 12 to to IN cord-319626-f1b3ygg0 49 13 the the DT cord-319626-f1b3ygg0 49 14 plasma plasma NN cord-319626-f1b3ygg0 49 15 membrane membrane NN cord-319626-f1b3ygg0 49 16 where where WRB cord-319626-f1b3ygg0 49 17 it -PRON- PRP cord-319626-f1b3ygg0 49 18 helps help VBZ cord-319626-f1b3ygg0 49 19 spread spread VB cord-319626-f1b3ygg0 49 20 the the DT cord-319626-f1b3ygg0 49 21 infection infection NN cord-319626-f1b3ygg0 49 22 by by IN cord-319626-f1b3ygg0 49 23 inducing induce VBG cord-319626-f1b3ygg0 49 24 the the DT cord-319626-f1b3ygg0 49 25 fusion fusion NN cord-319626-f1b3ygg0 49 26 of of IN cord-319626-f1b3ygg0 49 27 infected infected JJ cord-319626-f1b3ygg0 49 28 cells cell NNS cord-319626-f1b3ygg0 49 29 with with IN cord-319626-f1b3ygg0 49 30 contiguous contiguous JJ cord-319626-f1b3ygg0 49 31 uninfected uninfected JJ cord-319626-f1b3ygg0 49 32 cells cell NNS cord-319626-f1b3ygg0 49 33 ( ( -LRB- cord-319626-f1b3ygg0 49 34 Sturman Sturman NNP cord-319626-f1b3ygg0 49 35 and and CC cord-319626-f1b3ygg0 49 36 Holmes Holmes NNP cord-319626-f1b3ygg0 49 37 , , , cord-319626-f1b3ygg0 49 38 1983 1983 CD cord-319626-f1b3ygg0 49 39 ) ) -RRB- cord-319626-f1b3ygg0 49 40 . . . cord-319626-f1b3ygg0 50 1 Reduced reduce VBN cord-319626-f1b3ygg0 50 2 temperature temperature NN cord-319626-f1b3ygg0 50 3 has have VBZ cord-319626-f1b3ygg0 50 4 proven prove VBN cord-319626-f1b3ygg0 50 5 to to TO cord-319626-f1b3ygg0 50 6 be be VB cord-319626-f1b3ygg0 50 7 a a DT cord-319626-f1b3ygg0 50 8 very very RB cord-319626-f1b3ygg0 50 9 useful useful JJ cord-319626-f1b3ygg0 50 10 tool tool NN cord-319626-f1b3ygg0 50 11 in in IN cord-319626-f1b3ygg0 50 12 analyzing analyze VBG cord-319626-f1b3ygg0 50 13 compartments compartment NNS cord-319626-f1b3ygg0 50 14 on on IN cord-319626-f1b3ygg0 50 15 the the DT cord-319626-f1b3ygg0 50 16 exocytic exocytic JJ cord-319626-f1b3ygg0 50 17 transport transport NN cord-319626-f1b3ygg0 50 18 pathway pathway NN cord-319626-f1b3ygg0 50 19 . . . cord-319626-f1b3ygg0 51 1 Different different JJ cord-319626-f1b3ygg0 51 2 steps step NNS cord-319626-f1b3ygg0 51 3 are be VBP cord-319626-f1b3ygg0 51 4 differentially differentially RB cord-319626-f1b3ygg0 51 5 sensitive sensitive JJ cord-319626-f1b3ygg0 51 6 so so IN cord-319626-f1b3ygg0 51 7 that that IN cord-319626-f1b3ygg0 51 8 proteins protein NNS cord-319626-f1b3ygg0 51 9 accumulate accumulate VBP cord-319626-f1b3ygg0 51 10 at at IN cord-319626-f1b3ygg0 51 11 different different JJ cord-319626-f1b3ygg0 51 12 stages stage NNS cord-319626-f1b3ygg0 51 13 . . . cord-319626-f1b3ygg0 52 1 This this DT cord-319626-f1b3ygg0 52 2 has have VBZ cord-319626-f1b3ygg0 52 3 led lead VBN cord-319626-f1b3ygg0 52 4 to to IN cord-319626-f1b3ygg0 52 5 the the DT cord-319626-f1b3ygg0 52 6 morphological morphological JJ cord-319626-f1b3ygg0 52 7 characterization characterization NN cord-319626-f1b3ygg0 52 8 of of IN cord-319626-f1b3ygg0 52 9 a a DT cord-319626-f1b3ygg0 52 10 compartment compartment NN cord-319626-f1b3ygg0 52 11 similar similar JJ cord-319626-f1b3ygg0 52 12 to to IN cord-319626-f1b3ygg0 52 13 the the DT cord-319626-f1b3ygg0 52 14 coronavirus coronavirus NN cord-319626-f1b3ygg0 52 15 budding bud VBG cord-319626-f1b3ygg0 52 16 compartment compartment NN cord-319626-f1b3ygg0 52 17 , , , cord-319626-f1b3ygg0 52 18 but but CC cord-319626-f1b3ygg0 52 19 found find VBN cord-319626-f1b3ygg0 52 20 in in IN cord-319626-f1b3ygg0 52 21 baby baby NN cord-319626-f1b3ygg0 52 22 hamster hamster NN cord-319626-f1b3ygg0 52 23 kidney kidney NN cord-319626-f1b3ygg0 52 24 ( ( -LRB- cord-319626-f1b3ygg0 52 25 BHK BHK NNP cord-319626-f1b3ygg0 52 26 ) ) -RRB- cord-319626-f1b3ygg0 52 27 cells cell NNS cord-319626-f1b3ygg0 52 28 infected infect VBN cord-319626-f1b3ygg0 52 29 with with IN cord-319626-f1b3ygg0 52 30 Semliki Semliki NNP cord-319626-f1b3ygg0 52 31 Forest Forest NNP cord-319626-f1b3ygg0 52 32 virus virus NN cord-319626-f1b3ygg0 52 33 and and CC cord-319626-f1b3ygg0 52 34 incubated incubate VBD cord-319626-f1b3ygg0 52 35 at at IN cord-319626-f1b3ygg0 52 36 15 15 CD cord-319626-f1b3ygg0 52 37 ° ° NN cord-319626-f1b3ygg0 52 38 C c NN cord-319626-f1b3ygg0 52 39 ( ( -LRB- cord-319626-f1b3ygg0 52 40 Saraste Saraste NNP cord-319626-f1b3ygg0 52 41 and and CC cord-319626-f1b3ygg0 52 42 Kuismanen Kuismanen NNP cord-319626-f1b3ygg0 52 43 , , , cord-319626-f1b3ygg0 52 44 1984 1984 CD cord-319626-f1b3ygg0 52 45 ) ) -RRB- cord-319626-f1b3ygg0 52 46 . . . cord-319626-f1b3ygg0 53 1 Here here RB cord-319626-f1b3ygg0 53 2 we -PRON- PRP cord-319626-f1b3ygg0 53 3 exploit exploit VBP cord-319626-f1b3ygg0 53 4 reduced reduce VBN cord-319626-f1b3ygg0 53 5 temperature temperature NN cord-319626-f1b3ygg0 53 6 to to TO cord-319626-f1b3ygg0 53 7 prevent prevent VB cord-319626-f1b3ygg0 53 8 movement movement NN cord-319626-f1b3ygg0 53 9 of of IN cord-319626-f1b3ygg0 53 10 coronavirus coronavirus NN cord-319626-f1b3ygg0 53 11 and and CC cord-319626-f1b3ygg0 53 12 analyze analyze VB cord-319626-f1b3ygg0 53 13 the the DT cord-319626-f1b3ygg0 53 14 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 53 15 bound bind VBN cord-319626-f1b3ygg0 53 16 to to IN cord-319626-f1b3ygg0 53 17 E1 E1 NNP cord-319626-f1b3ygg0 53 18 on on IN cord-319626-f1b3ygg0 53 19 the the DT cord-319626-f1b3ygg0 53 20 arrested arrest VBN cord-319626-f1b3ygg0 53 21 virus virus NN cord-319626-f1b3ygg0 53 22 . . . cord-319626-f1b3ygg0 54 1 We -PRON- PRP cord-319626-f1b3ygg0 54 2 also also RB cord-319626-f1b3ygg0 54 3 present present VBP cord-319626-f1b3ygg0 54 4 evidence evidence NN cord-319626-f1b3ygg0 54 5 showing show VBG cord-319626-f1b3ygg0 54 6 that that IN cord-319626-f1b3ygg0 54 7 the the DT cord-319626-f1b3ygg0 54 8 budding bud VBG cord-319626-f1b3ygg0 54 9 compartment compartment NN cord-319626-f1b3ygg0 54 10 , , , cord-319626-f1b3ygg0 54 11 which which WDT cord-319626-f1b3ygg0 54 12 by by IN cord-319626-f1b3ygg0 54 13 morphological morphological JJ cord-319626-f1b3ygg0 54 14 criteria criterion NNS cord-319626-f1b3ygg0 54 15 is be VBZ cord-319626-f1b3ygg0 54 16 clearly clearly RB cord-319626-f1b3ygg0 54 17 related relate VBN cord-319626-f1b3ygg0 54 18 to to IN cord-319626-f1b3ygg0 54 19 transitional transitional JJ cord-319626-f1b3ygg0 54 20 elements element NNS cord-319626-f1b3ygg0 54 21 and and CC cord-319626-f1b3ygg0 54 22 transitional transitional JJ cord-319626-f1b3ygg0 54 23 vesicles vesicle NNS cord-319626-f1b3ygg0 54 24 , , , cord-319626-f1b3ygg0 54 25 as as IN cord-319626-f1b3ygg0 54 26 classically classically RB cord-319626-f1b3ygg0 54 27 defined define VBN cord-319626-f1b3ygg0 54 28 in in IN cord-319626-f1b3ygg0 54 29 exocrine exocrine JJ cord-319626-f1b3ygg0 54 30 pancreatic pancreatic NN cord-319626-f1b3ygg0 54 31 cells cell NNS cord-319626-f1b3ygg0 54 32 by by IN cord-319626-f1b3ygg0 54 33 the the DT cord-319626-f1b3ygg0 54 34 work work NN cord-319626-f1b3ygg0 54 35 of of IN cord-319626-f1b3ygg0 54 36 Palade Palade NNP cord-319626-f1b3ygg0 54 37 and and CC cord-319626-f1b3ygg0 54 38 colleagues colleague NNS cord-319626-f1b3ygg0 54 39 ( ( -LRB- cord-319626-f1b3ygg0 54 40 Caro Caro NNP cord-319626-f1b3ygg0 54 41 and and CC cord-319626-f1b3ygg0 54 42 Palade Palade NNP cord-319626-f1b3ygg0 54 43 , , , cord-319626-f1b3ygg0 54 44 1964 1964 CD cord-319626-f1b3ygg0 54 45 ; ; : cord-319626-f1b3ygg0 54 46 Jamieson Jamieson NNP cord-319626-f1b3ygg0 54 47 and and CC cord-319626-f1b3ygg0 54 48 Palade Palade NNP cord-319626-f1b3ygg0 54 49 , , , cord-319626-f1b3ygg0 54 50 1967 1967 CD cord-319626-f1b3ygg0 54 51 ) ) -RRB- cord-319626-f1b3ygg0 54 52 , , , cord-319626-f1b3ygg0 54 53 is be VBZ cord-319626-f1b3ygg0 54 54 not not RB cord-319626-f1b3ygg0 54 55 created create VBN cord-319626-f1b3ygg0 54 56 by by IN cord-319626-f1b3ygg0 54 57 the the DT cord-319626-f1b3ygg0 54 58 process process NN cord-319626-f1b3ygg0 54 59 of of IN cord-319626-f1b3ygg0 54 60 infection infection NN cord-319626-f1b3ygg0 54 61 but but CC cord-319626-f1b3ygg0 54 62 exists exist VBZ cord-319626-f1b3ygg0 54 63 in in IN cord-319626-f1b3ygg0 54 64 the the DT cord-319626-f1b3ygg0 54 65 uninfected uninfected JJ cord-319626-f1b3ygg0 54 66 cell cell NN cord-319626-f1b3ygg0 54 67 . . . cord-319626-f1b3ygg0 55 1 A a DT cord-319626-f1b3ygg0 55 2 physiological physiological JJ cord-319626-f1b3ygg0 55 3 function function NN cord-319626-f1b3ygg0 55 4 of of IN cord-319626-f1b3ygg0 55 5 this this DT cord-319626-f1b3ygg0 55 6 compartment compartment NN cord-319626-f1b3ygg0 55 7 appears appear VBZ cord-319626-f1b3ygg0 55 8 to to TO cord-319626-f1b3ygg0 55 9 be be VB cord-319626-f1b3ygg0 55 10 the the DT cord-319626-f1b3ygg0 55 11 addition addition NN cord-319626-f1b3ygg0 55 12 of of IN cord-319626-f1b3ygg0 55 13 GalNAc GalNAc NNP cord-319626-f1b3ygg0 55 14 , , , cord-319626-f1b3ygg0 55 15 implying imply VBG cord-319626-f1b3ygg0 55 16 that that IN cord-319626-f1b3ygg0 55 17 it -PRON- PRP cord-319626-f1b3ygg0 55 18 contains contain VBZ cord-319626-f1b3ygg0 55 19 the the DT cord-319626-f1b3ygg0 55 20 enzyme enzyme NN cord-319626-f1b3ygg0 55 21 which which WDT cord-319626-f1b3ygg0 55 22 adds add VBZ cord-319626-f1b3ygg0 55 23 this this DT cord-319626-f1b3ygg0 55 24 sugar sugar NN cord-319626-f1b3ygg0 55 25 to to IN cord-319626-f1b3ygg0 55 26 serine serine NN cord-319626-f1b3ygg0 55 27 and and CC cord-319626-f1b3ygg0 55 28 threonine threonine NN cord-319626-f1b3ygg0 55 29 residues residue NNS cord-319626-f1b3ygg0 55 30 . . . cord-319626-f1b3ygg0 56 1 N n NN cord-319626-f1b3ygg0 56 2 - - HYPH cord-319626-f1b3ygg0 56 3 acetyl acetyl NNP cord-319626-f1b3ygg0 56 4 [ [ -LRB- cord-319626-f1b3ygg0 56 5 4,5,6,7,8,9 4,5,6,7,8,9 CD cord-319626-f1b3ygg0 56 6 - - HYPH cord-319626-f1b3ygg0 56 7 14C 14c NN cord-319626-f1b3ygg0 56 8 ] ] -RRB- cord-319626-f1b3ygg0 56 9 _SP cord-319626-f1b3ygg0 57 1 MHV MHV NNP cord-319626-f1b3ygg0 57 2 - - HYPH cord-319626-f1b3ygg0 57 3 A59 A59 NNP cord-319626-f1b3ygg0 57 4 was be VBD cord-319626-f1b3ygg0 57 5 propagated propagate VBN cord-319626-f1b3ygg0 57 6 on on IN cord-319626-f1b3ygg0 57 7 sac(- sac(- NNP cord-319626-f1b3ygg0 57 8 ) ) -RRB- cord-319626-f1b3ygg0 57 9 cells cell NNS cord-319626-f1b3ygg0 57 10 ( ( -LRB- cord-319626-f1b3ygg0 57 11 Tooze Tooze NNP cord-319626-f1b3ygg0 57 12 et et NNP cord-319626-f1b3ygg0 57 13 al al NNP cord-319626-f1b3ygg0 57 14 . . NNP cord-319626-f1b3ygg0 57 15 , , , cord-319626-f1b3ygg0 57 16 1984 1984 CD cord-319626-f1b3ygg0 57 17 ) ) -RRB- cord-319626-f1b3ygg0 57 18 and and CC cord-319626-f1b3ygg0 57 19 titered titere VBN cord-319626-f1b3ygg0 57 20 by by IN cord-319626-f1b3ygg0 57 21 plaque plaque NN cord-319626-f1b3ygg0 57 22 titration titration NN cord-319626-f1b3ygg0 57 23 on on IN cord-319626-f1b3ygg0 57 24 confluent confluent JJ cord-319626-f1b3ygg0 57 25 monolayers monolayer NNS cord-319626-f1b3ygg0 57 26 of of IN cord-319626-f1b3ygg0 57 27 L L NNP cord-319626-f1b3ygg0 57 28 cells cell NNS cord-319626-f1b3ygg0 57 29 as as IN cord-319626-f1b3ygg0 57 30 follows follow VBZ cord-319626-f1b3ygg0 57 31 . . . cord-319626-f1b3ygg0 58 1 After after IN cord-319626-f1b3ygg0 58 2 infection infection NN cord-319626-f1b3ygg0 58 3 for for IN cord-319626-f1b3ygg0 58 4 1 1 CD cord-319626-f1b3ygg0 58 5 h h NN cord-319626-f1b3ygg0 58 6 at at IN cord-319626-f1b3ygg0 58 7 37 37 CD cord-319626-f1b3ygg0 58 8 ° ° NN cord-319626-f1b3ygg0 58 9 C c NN cord-319626-f1b3ygg0 58 10 , , , cord-319626-f1b3ygg0 58 11 the the DT cord-319626-f1b3ygg0 58 12 monolayers monolayer NNS cord-319626-f1b3ygg0 58 13 were be VBD cord-319626-f1b3ygg0 58 14 overlaid overlay VBN cord-319626-f1b3ygg0 58 15 with with IN cord-319626-f1b3ygg0 58 16 2 2 CD cord-319626-f1b3ygg0 58 17 % % NN cord-319626-f1b3ygg0 58 18 carboxymethyl carboxymethyl NN cord-319626-f1b3ygg0 58 19 cellulose cellulose NN cord-319626-f1b3ygg0 58 20 and and CC cord-319626-f1b3ygg0 58 21 incubated incubate VBN cord-319626-f1b3ygg0 58 22 for for IN cord-319626-f1b3ygg0 58 23 24 24 CD cord-319626-f1b3ygg0 58 24 h h NN cord-319626-f1b3ygg0 58 25 at at IN cord-319626-f1b3ygg0 58 26 37 37 CD cord-319626-f1b3ygg0 58 27 ° ° NN cord-319626-f1b3ygg0 58 28 C c NN cord-319626-f1b3ygg0 58 29 in in IN cord-319626-f1b3ygg0 58 30 95 95 CD cord-319626-f1b3ygg0 58 31 % % NN cord-319626-f1b3ygg0 58 32 air/5 air/5 NN cord-319626-f1b3ygg0 58 33 % % NN cord-319626-f1b3ygg0 58 34 COs COs NNPS cord-319626-f1b3ygg0 58 35 incubator incubator NN cord-319626-f1b3ygg0 58 36 . . . cord-319626-f1b3ygg0 59 1 The the DT cord-319626-f1b3ygg0 59 2 monolayers monolayer NNS cord-319626-f1b3ygg0 59 3 were be VBD cord-319626-f1b3ygg0 59 4 then then RB cord-319626-f1b3ygg0 59 5 fixed fix VBN cord-319626-f1b3ygg0 59 6 and and CC cord-319626-f1b3ygg0 59 7 stained stain VBN cord-319626-f1b3ygg0 59 8 with with IN cord-319626-f1b3ygg0 59 9 35 35 CD cord-319626-f1b3ygg0 59 10 mM mm CD cord-319626-f1b3ygg0 59 11 Tris Tris NNP cord-319626-f1b3ygg0 59 12 - - HYPH cord-319626-f1b3ygg0 59 13 Cl Cl NNP cord-319626-f1b3ygg0 59 14 buffer buffer NN cord-319626-f1b3ygg0 59 15 , , , cord-319626-f1b3ygg0 59 16 pH pH NNP cord-319626-f1b3ygg0 59 17 7.2 7.2 CD cord-319626-f1b3ygg0 59 18 , , , cord-319626-f1b3ygg0 59 19 containing contain VBG cord-319626-f1b3ygg0 59 20 0.25 0.25 CD cord-319626-f1b3ygg0 59 21 % % NN cord-319626-f1b3ygg0 59 22 ( ( -LRB- cord-319626-f1b3ygg0 59 23 wt wt NN cord-319626-f1b3ygg0 59 24 / / SYM cord-319626-f1b3ygg0 59 25 vol vol NNP cord-319626-f1b3ygg0 59 26 ) ) -RRB- cord-319626-f1b3ygg0 59 27 crystal crystal NNP cord-319626-f1b3ygg0 59 28 violet violet NN cord-319626-f1b3ygg0 59 29 , , , cord-319626-f1b3ygg0 59 30 1.75 1.75 CD cord-319626-f1b3ygg0 59 31 % % NN cord-319626-f1b3ygg0 59 32 ( ( -LRB- cord-319626-f1b3ygg0 59 33 vol vol NNP cord-319626-f1b3ygg0 59 34 / / SYM cord-319626-f1b3ygg0 59 35 vol vol NN cord-319626-f1b3ygg0 59 36 ) ) -RRB- cord-319626-f1b3ygg0 59 37 formaldehyde formaldehyde NN cord-319626-f1b3ygg0 59 38 , , , cord-319626-f1b3ygg0 59 39 10 10 CD cord-319626-f1b3ygg0 59 40 % % NN cord-319626-f1b3ygg0 59 41 ( ( -LRB- cord-319626-f1b3ygg0 59 42 vol vol NNP cord-319626-f1b3ygg0 59 43 / / SYM cord-319626-f1b3ygg0 59 44 vol vol NNP cord-319626-f1b3ygg0 59 45 ) ) -RRB- cord-319626-f1b3ygg0 59 46 MeOH MeOH NNP cord-319626-f1b3ygg0 59 47 , , , cord-319626-f1b3ygg0 59 48 and and CC cord-319626-f1b3ygg0 59 49 0.5 0.5 CD cord-319626-f1b3ygg0 59 50 % % NN cord-319626-f1b3ygg0 59 51 ( ( -LRB- cord-319626-f1b3ygg0 59 52 wt wt NN cord-319626-f1b3ygg0 59 53 / / SYM cord-319626-f1b3ygg0 59 54 vol vol NN cord-319626-f1b3ygg0 59 55 ) ) -RRB- cord-319626-f1b3ygg0 59 56 CaCI2 CaCI2 NNP cord-319626-f1b3ygg0 59 57 ( ( -LRB- cord-319626-f1b3ygg0 59 58 K K NNP cord-319626-f1b3ygg0 59 59 ~ ~ NNP cord-319626-f1b3ygg0 59 60 ari ari NNP cord-319626-f1b3ygg0 59 61 ~ ~ NFP cord-319626-f1b3ygg0 59 62 inen inen NNP cord-319626-f1b3ygg0 59 63 et et NNP cord-319626-f1b3ygg0 59 64 al al NNP cord-319626-f1b3ygg0 59 65 . . NNP cord-319626-f1b3ygg0 59 66 , , , cord-319626-f1b3ygg0 59 67 1969 1969 CD cord-319626-f1b3ygg0 59 68 ) ) -RRB- cord-319626-f1b3ygg0 59 69 . . . cord-319626-f1b3ygg0 60 1 Sac(- sac(- LS cord-319626-f1b3ygg0 60 2 ) ) -RRB- cord-319626-f1b3ygg0 60 3 cells cell NNS cord-319626-f1b3ygg0 60 4 grown grow VBN cord-319626-f1b3ygg0 60 5 on on IN cord-319626-f1b3ygg0 60 6 coverslips coverslip NNS cord-319626-f1b3ygg0 60 7 and and CC cord-319626-f1b3ygg0 60 8 infected infect VBN cord-319626-f1b3ygg0 60 9 with with IN cord-319626-f1b3ygg0 60 10 MHV MHV NNP cord-319626-f1b3ygg0 60 11 - - HYPH cord-319626-f1b3ygg0 60 12 A59 A59 NNP cord-319626-f1b3ygg0 60 13 were be VBD cord-319626-f1b3ygg0 60 14 fixed fix VBN cord-319626-f1b3ygg0 60 15 with with IN cord-319626-f1b3ygg0 60 16 3 3 CD cord-319626-f1b3ygg0 60 17 % % NN cord-319626-f1b3ygg0 60 18 formaldehyde formaldehyde NN cord-319626-f1b3ygg0 60 19 and and CC cord-319626-f1b3ygg0 60 20 labeled label VBN cord-319626-f1b3ygg0 60 21 ( ( -LRB- cord-319626-f1b3ygg0 60 22 Tooze Tooze NNP cord-319626-f1b3ygg0 60 23 et et NNP cord-319626-f1b3ygg0 60 24 al al NNP cord-319626-f1b3ygg0 60 25 . . NNP cord-319626-f1b3ygg0 60 26 , , , cord-319626-f1b3ygg0 60 27 1984 1984 CD cord-319626-f1b3ygg0 60 28 ) ) -RRB- cord-319626-f1b3ygg0 60 29 using use VBG cord-319626-f1b3ygg0 60 30 either either CC cord-319626-f1b3ygg0 60 31 a a DT cord-319626-f1b3ygg0 60 32 mAb mab NN cord-319626-f1b3ygg0 60 33 directed direct VBN cord-319626-f1b3ygg0 60 34 against against IN cord-319626-f1b3ygg0 60 35 E1 E1 NNP cord-319626-f1b3ygg0 60 36 ( ( -LRB- cord-319626-f1b3ygg0 60 37 4 4 CD cord-319626-f1b3ygg0 60 38 - - SYM cord-319626-f1b3ygg0 60 39 17-M1-F4-M1 17-m1-f4-m1 CD cord-319626-f1b3ygg0 60 40 ; ; : cord-319626-f1b3ygg0 60 41 gift gift NN cord-319626-f1b3ygg0 60 42 of of IN cord-319626-f1b3ygg0 60 43 M. M. NNP cord-319626-f1b3ygg0 60 44 Koolen Koolen NNP cord-319626-f1b3ygg0 60 45 , , , cord-319626-f1b3ygg0 60 46 Utrecht Utrecht NNP cord-319626-f1b3ygg0 60 47 , , , cord-319626-f1b3ygg0 60 48 and and CC cord-319626-f1b3ygg0 60 49 A. A. NNP cord-319626-f1b3ygg0 60 50 Osterhus Osterhus NNP cord-319626-f1b3ygg0 60 51 , , , cord-319626-f1b3ygg0 60 52 National National NNP cord-319626-f1b3ygg0 60 53 Institute Institute NNP cord-319626-f1b3ygg0 60 54 of of IN cord-319626-f1b3ygg0 60 55 Public Public NNP cord-319626-f1b3ygg0 60 56 Health Health NNP cord-319626-f1b3ygg0 60 57 , , , cord-319626-f1b3ygg0 60 58 Bilthoven Bilthoven NNP cord-319626-f1b3ygg0 60 59 , , , cord-319626-f1b3ygg0 60 60 Holland Holland NNP cord-319626-f1b3ygg0 60 61 ) ) -RRB- cord-319626-f1b3ygg0 60 62 or or CC cord-319626-f1b3ygg0 60 63 an an DT cord-319626-f1b3ygg0 60 64 anti anti JJ cord-319626-f1b3ygg0 60 65 - - JJ cord-319626-f1b3ygg0 60 66 body body NN cord-319626-f1b3ygg0 60 67 directed direct VBN cord-319626-f1b3ygg0 60 68 against against IN cord-319626-f1b3ygg0 60 69 an an DT cord-319626-f1b3ygg0 60 70 endogenous endogenous JJ cord-319626-f1b3ygg0 60 71 Golgi Golgi NNP cord-319626-f1b3ygg0 60 72 protein protein NN cord-319626-f1b3ygg0 60 73 ( ( -LRB- cord-319626-f1b3ygg0 60 74 Burke Burke NNP cord-319626-f1b3ygg0 60 75 et et NNP cord-319626-f1b3ygg0 60 76 al al NNP cord-319626-f1b3ygg0 60 77 . . NNP cord-319626-f1b3ygg0 60 78 , , , cord-319626-f1b3ygg0 60 79 1982 1982 CD cord-319626-f1b3ygg0 60 80 ) ) -RRB- cord-319626-f1b3ygg0 60 81 . . . cord-319626-f1b3ygg0 61 1 The the DT cord-319626-f1b3ygg0 61 2 second second JJ cord-319626-f1b3ygg0 61 3 antibody antibody NN cord-319626-f1b3ygg0 61 4 ( ( -LRB- cord-319626-f1b3ygg0 61 5 rabbit rabbit NN cord-319626-f1b3ygg0 61 6 anti anti JJ cord-319626-f1b3ygg0 61 7 - - JJ cord-319626-f1b3ygg0 61 8 mouse mouse NN cord-319626-f1b3ygg0 61 9 ) ) -RRB- cord-319626-f1b3ygg0 61 10 , , , cord-319626-f1b3ygg0 61 11 kindly kindly RB cord-319626-f1b3ygg0 61 12 provided provide VBN cord-319626-f1b3ygg0 61 13 by by IN cord-319626-f1b3ygg0 61 14 Dr. Dr. NNP cord-319626-f1b3ygg0 61 15 B. B. NNP cord-319626-f1b3ygg0 61 16 Burke Burke NNP cord-319626-f1b3ygg0 61 17 , , , cord-319626-f1b3ygg0 61 18 European European NNP cord-319626-f1b3ygg0 61 19 Molecular Molecular NNP cord-319626-f1b3ygg0 61 20 Biology Biology NNP cord-319626-f1b3ygg0 61 21 Laboratory Laboratory NNP cord-319626-f1b3ygg0 61 22 , , , cord-319626-f1b3ygg0 61 23 Heidelberg Heidelberg NNP cord-319626-f1b3ygg0 61 24 , , , cord-319626-f1b3ygg0 61 25 FRG FRG NNP cord-319626-f1b3ygg0 61 26 , , , cord-319626-f1b3ygg0 61 27 was be VBD cord-319626-f1b3ygg0 61 28 affinity affinity NN cord-319626-f1b3ygg0 61 29 purified purify VBN cord-319626-f1b3ygg0 61 30 and and CC cord-319626-f1b3ygg0 61 31 conjugated conjugate VBN cord-319626-f1b3ygg0 61 32 to to IN cord-319626-f1b3ygg0 61 33 rhodamine rhodamine NN cord-319626-f1b3ygg0 61 34 . . . cord-319626-f1b3ygg0 62 1 The the DT cord-319626-f1b3ygg0 62 2 fixed fix VBN cord-319626-f1b3ygg0 62 3 and and CC cord-319626-f1b3ygg0 62 4 labeled label VBN cord-319626-f1b3ygg0 62 5 coverslips coverslip NNS cord-319626-f1b3ygg0 62 6 were be VBD cord-319626-f1b3ygg0 62 7 mounted mount VBN cord-319626-f1b3ygg0 62 8 in in IN cord-319626-f1b3ygg0 62 9 Moviol Moviol NNP cord-319626-f1b3ygg0 62 10 and and CC cord-319626-f1b3ygg0 62 11 viewed view VBN cord-319626-f1b3ygg0 62 12 by by IN cord-319626-f1b3ygg0 62 13 epifluorescence epifluorescence NN cord-319626-f1b3ygg0 62 14 on on IN cord-319626-f1b3ygg0 62 15 a a DT cord-319626-f1b3ygg0 62 16 Zeiss Zeiss NNP cord-319626-f1b3ygg0 62 17 photomicroscope photomicroscope NN cord-319626-f1b3ygg0 62 18 III III NNP cord-319626-f1b3ygg0 62 19 . . . cord-319626-f1b3ygg0 63 1 Infected infected JJ cord-319626-f1b3ygg0 63 2 sac(- sac(- NNP cord-319626-f1b3ygg0 63 3 ) ) -RRB- cord-319626-f1b3ygg0 63 4 cells cell NNS cord-319626-f1b3ygg0 63 5 in in IN cord-319626-f1b3ygg0 63 6 35-mm 35-mm CD cord-319626-f1b3ygg0 63 7 dishes dish NNS cord-319626-f1b3ygg0 63 8 were be VBD cord-319626-f1b3ygg0 63 9 fixed fix VBN cord-319626-f1b3ygg0 63 10 , , , cord-319626-f1b3ygg0 63 11 dehydrated dehydrated JJ cord-319626-f1b3ygg0 63 12 , , , cord-319626-f1b3ygg0 63 13 and and CC cord-319626-f1b3ygg0 63 14 embedded embed VBN cord-319626-f1b3ygg0 63 15 in in IN cord-319626-f1b3ygg0 63 16 Epon Epon NNP cord-319626-f1b3ygg0 63 17 as as IN cord-319626-f1b3ygg0 63 18 previously previously RB cord-319626-f1b3ygg0 63 19 detailed detail VBN cord-319626-f1b3ygg0 64 1 ( ( -LRB- cord-319626-f1b3ygg0 64 2 Tooze Tooze NNP cord-319626-f1b3ygg0 64 3 et et NNP cord-319626-f1b3ygg0 64 4 al al NNP cord-319626-f1b3ygg0 64 5 . . NNP cord-319626-f1b3ygg0 64 6 , , , cord-319626-f1b3ygg0 64 7 1984 1984 CD cord-319626-f1b3ygg0 64 8 ) ) -RRB- cord-319626-f1b3ygg0 64 9 . . . cord-319626-f1b3ygg0 65 1 The the DT cord-319626-f1b3ygg0 65 2 anti anti JJ cord-319626-f1b3ygg0 65 3 - - JJ cord-319626-f1b3ygg0 65 4 E1 e1 JJ cord-319626-f1b3ygg0 65 5 antibody antibody NN cord-319626-f1b3ygg0 65 6 ( ( -LRB- cord-319626-f1b3ygg0 65 7 4 4 CD cord-319626-f1b3ygg0 65 8 - - SYM cord-319626-f1b3ygg0 65 9 17-M1-F4-M1 17-m1-f4-m1 CD cord-319626-f1b3ygg0 65 10 ) ) -RRB- cord-319626-f1b3ygg0 65 11 was be VBD cord-319626-f1b3ygg0 65 12 used use VBN cord-319626-f1b3ygg0 65 13 for for IN cord-319626-f1b3ygg0 65 14 immunoperoxidase immunoperoxidase NN cord-319626-f1b3ygg0 65 15 labeling labeling NN cord-319626-f1b3ygg0 65 16 after after IN cord-319626-f1b3ygg0 65 17 our -PRON- PRP$ cord-319626-f1b3ygg0 65 18 standard standard JJ cord-319626-f1b3ygg0 65 19 protocol protocol NN cord-319626-f1b3ygg0 66 1 ( ( -LRB- cord-319626-f1b3ygg0 66 2 Tooze Tooze NNP cord-319626-f1b3ygg0 66 3 et et NNP cord-319626-f1b3ygg0 66 4 al al NNP cord-319626-f1b3ygg0 66 5 . . NNP cord-319626-f1b3ygg0 66 6 , , , cord-319626-f1b3ygg0 66 7 1984 1984 CD cord-319626-f1b3ygg0 66 8 ) ) -RRB- cord-319626-f1b3ygg0 66 9 . . . cord-319626-f1b3ygg0 67 1 Infected infected JJ cord-319626-f1b3ygg0 67 2 sac(- sac(- NNP cord-319626-f1b3ygg0 67 3 ) ) -RRB- cord-319626-f1b3ygg0 67 4 cells cell NNS cord-319626-f1b3ygg0 67 5 , , , cord-319626-f1b3ygg0 67 6 in in IN cord-319626-f1b3ygg0 67 7 35-mm 35-mm CD cord-319626-f1b3ygg0 67 8 dishes dish NNS cord-319626-f1b3ygg0 67 9 , , , cord-319626-f1b3ygg0 67 10 were be VBD cord-319626-f1b3ygg0 67 11 either either CC cord-319626-f1b3ygg0 67 12 pulse pulse NN cord-319626-f1b3ygg0 67 13 labeled label VBN cord-319626-f1b3ygg0 67 14 or or CC cord-319626-f1b3ygg0 67 15 continously continously RB cord-319626-f1b3ygg0 67 16 labeled label VBN cord-319626-f1b3ygg0 67 17 with with IN cord-319626-f1b3ygg0 67 18 [ [ -LRB- cord-319626-f1b3ygg0 67 19 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 67 20 . . . cord-319626-f1b3ygg0 68 1 After after IN cord-319626-f1b3ygg0 68 2 washing wash VBG cord-319626-f1b3ygg0 68 3 three three CD cord-319626-f1b3ygg0 68 4 times time NNS cord-319626-f1b3ygg0 68 5 in in IN cord-319626-f1b3ygg0 68 6 methionine methionine NN cord-319626-f1b3ygg0 68 7 - - HYPH cord-319626-f1b3ygg0 68 8 free free JJ cord-319626-f1b3ygg0 68 9 modified modify VBN cord-319626-f1b3ygg0 68 10 Eagle Eagle NNP cord-319626-f1b3ygg0 68 11 's 's POS cord-319626-f1b3ygg0 68 12 medium medium NN cord-319626-f1b3ygg0 68 13 ( ( -LRB- cord-319626-f1b3ygg0 68 14 MEM mem NN cord-319626-f1b3ygg0 68 15 - - HYPH cord-319626-f1b3ygg0 68 16 met met NNP cord-319626-f1b3ygg0 68 17 ) ) -RRB- cord-319626-f1b3ygg0 68 18 , , , cord-319626-f1b3ygg0 68 19 cells cell NNS cord-319626-f1b3ygg0 68 20 were be VBD cord-319626-f1b3ygg0 68 21 labeled label VBN cord-319626-f1b3ygg0 68 22 with with IN cord-319626-f1b3ygg0 68 23 100 100 CD cord-319626-f1b3ygg0 68 24 - - HYPH cord-319626-f1b3ygg0 68 25 250 250 CD cord-319626-f1b3ygg0 68 26 ~tCi ~tCi NFP cord-319626-f1b3ygg0 69 1 [ [ -LRB- cord-319626-f1b3ygg0 69 2 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 69 3 for for IN cord-319626-f1b3ygg0 69 4 5 5 CD cord-319626-f1b3ygg0 69 5 - - SYM cord-319626-f1b3ygg0 69 6 10 10 CD cord-319626-f1b3ygg0 69 7 min min NN cord-319626-f1b3ygg0 69 8 at at IN cord-319626-f1b3ygg0 69 9 37 37 CD cord-319626-f1b3ygg0 69 10 ° ° NN cord-319626-f1b3ygg0 69 11 C c NN cord-319626-f1b3ygg0 69 12 , , , cord-319626-f1b3ygg0 69 13 or or CC cord-319626-f1b3ygg0 69 14 for for IN cord-319626-f1b3ygg0 69 15 longer long JJR cord-319626-f1b3ygg0 69 16 periods period NNS cord-319626-f1b3ygg0 69 17 at at IN cord-319626-f1b3ygg0 69 18 35 35 CD cord-319626-f1b3ygg0 69 19 , , , cord-319626-f1b3ygg0 69 20 31 31 CD cord-319626-f1b3ygg0 69 21 , , , cord-319626-f1b3ygg0 69 22 30 30 CD cord-319626-f1b3ygg0 69 23 , , , cord-319626-f1b3ygg0 69 24 25 25 CD cord-319626-f1b3ygg0 69 25 , , , cord-319626-f1b3ygg0 69 26 and and CC cord-319626-f1b3ygg0 69 27 20 20 CD cord-319626-f1b3ygg0 69 28 ° ° NN cord-319626-f1b3ygg0 69 29 C c NN cord-319626-f1b3ygg0 69 30 . . . cord-319626-f1b3ygg0 70 1 Some some DT cord-319626-f1b3ygg0 70 2 cells cell NNS cord-319626-f1b3ygg0 70 3 were be VBD cord-319626-f1b3ygg0 70 4 chased chase VBN cord-319626-f1b3ygg0 70 5 at at IN cord-319626-f1b3ygg0 70 6 37 37 CD cord-319626-f1b3ygg0 70 7 or or CC cord-319626-f1b3ygg0 70 8 31 31 CD cord-319626-f1b3ygg0 70 9 ° ° NN cord-319626-f1b3ygg0 70 10 C c NN cord-319626-f1b3ygg0 70 11 for for IN cord-319626-f1b3ygg0 70 12 various various JJ cord-319626-f1b3ygg0 70 13 times time NNS cord-319626-f1b3ygg0 70 14 by by IN cord-319626-f1b3ygg0 70 15 the the DT cord-319626-f1b3ygg0 70 16 addition addition NN cord-319626-f1b3ygg0 70 17 of of IN cord-319626-f1b3ygg0 70 18 growth growth NN cord-319626-f1b3ygg0 70 19 medium medium NN cord-319626-f1b3ygg0 70 20 containing contain VBG cord-319626-f1b3ygg0 70 21 1.5 1.5 CD cord-319626-f1b3ygg0 70 22 mg mg NNP cord-319626-f1b3ygg0 70 23 / / SYM cord-319626-f1b3ygg0 70 24 ml ml NN cord-319626-f1b3ygg0 70 25 of of IN cord-319626-f1b3ygg0 70 26 methionine methionine NN cord-319626-f1b3ygg0 70 27 , , , cord-319626-f1b3ygg0 71 1 10× 10× CD cord-319626-f1b3ygg0 71 2 the the DT cord-319626-f1b3ygg0 71 3 normal normal JJ cord-319626-f1b3ygg0 71 4 amount amount NN cord-319626-f1b3ygg0 71 5 . . . cord-319626-f1b3ygg0 72 1 For for IN cord-319626-f1b3ygg0 72 2 quantitation quantitation NN cord-319626-f1b3ygg0 72 3 of of IN cord-319626-f1b3ygg0 72 4 the the DT cord-319626-f1b3ygg0 72 5 number number NN cord-319626-f1b3ygg0 72 6 of of IN cord-319626-f1b3ygg0 72 7 El El NNP cord-319626-f1b3ygg0 72 8 molecules molecule NNS cord-319626-f1b3ygg0 72 9 labeled label VBD cord-319626-f1b3ygg0 72 10 [ [ -LRB- cord-319626-f1b3ygg0 72 11 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 72 12 with with IN cord-319626-f1b3ygg0 72 13 a a DT cord-319626-f1b3ygg0 72 14 defined define VBN cord-319626-f1b3ygg0 72 15 specific specific JJ cord-319626-f1b3ygg0 72 16 activity activity NN cord-319626-f1b3ygg0 72 17 was be VBD cord-319626-f1b3ygg0 72 18 used use VBN cord-319626-f1b3ygg0 72 19 . . . cord-319626-f1b3ygg0 73 1 The the DT cord-319626-f1b3ygg0 73 2 cells cell NNS cord-319626-f1b3ygg0 73 3 were'washed were'washed VBP cord-319626-f1b3ygg0 73 4 with with IN cord-319626-f1b3ygg0 73 5 ice ice NN cord-319626-f1b3ygg0 73 6 cold cold JJ cord-319626-f1b3ygg0 73 7 PBS PBS NNP cord-319626-f1b3ygg0 73 8 and and CC cord-319626-f1b3ygg0 73 9 extracted extract VBN cord-319626-f1b3ygg0 73 10 with with IN cord-319626-f1b3ygg0 73 11 1 1 CD cord-319626-f1b3ygg0 73 12 % % NN cord-319626-f1b3ygg0 73 13 Triton Triton NNP cord-319626-f1b3ygg0 73 14 X-114 X-114 NNS cord-319626-f1b3ygg0 73 15 ( ( -LRB- cord-319626-f1b3ygg0 73 16 Tx Tx NNP cord-319626-f1b3ygg0 73 17 - - HYPH cord-319626-f1b3ygg0 73 18 ll4 ll4 NNP cord-319626-f1b3ygg0 73 19 ) ) -RRB- cord-319626-f1b3ygg0 73 20 in in IN cord-319626-f1b3ygg0 73 21 PBS PBS NNP cord-319626-f1b3ygg0 73 22 containing contain VBG cord-319626-f1b3ygg0 73 23 40 40 CD cord-319626-f1b3ygg0 73 24 ~tg ~tg NNS cord-319626-f1b3ygg0 73 25 / / SYM cord-319626-f1b3ygg0 73 26 ml ml NN cord-319626-f1b3ygg0 73 27 phenylmethylsulfonyl phenylmethylsulfonyl NN cord-319626-f1b3ygg0 73 28 fluoride fluoride NN cord-319626-f1b3ygg0 73 29 ( ( -LRB- cord-319626-f1b3ygg0 73 30 PMSF PMSF NNP cord-319626-f1b3ygg0 73 31 ) ) -RRB- cord-319626-f1b3ygg0 73 32 at at IN cord-319626-f1b3ygg0 73 33 4 4 CD cord-319626-f1b3ygg0 73 34 ° ° NN cord-319626-f1b3ygg0 73 35 C c NN cord-319626-f1b3ygg0 73 36 . . . cord-319626-f1b3ygg0 74 1 The the DT cord-319626-f1b3ygg0 74 2 insoluble insoluble JJ cord-319626-f1b3ygg0 74 3 residue residue NN cord-319626-f1b3ygg0 74 4 was be VBD cord-319626-f1b3ygg0 74 5 removed remove VBN cord-319626-f1b3ygg0 74 6 by by IN cord-319626-f1b3ygg0 74 7 centrifugation centrifugation NN cord-319626-f1b3ygg0 74 8 at at IN cord-319626-f1b3ygg0 74 9 10,000 10,000 CD cord-319626-f1b3ygg0 74 10 g~ g~ CD cord-319626-f1b3ygg0 74 11 for for IN cord-319626-f1b3ygg0 74 12 5 5 CD cord-319626-f1b3ygg0 74 13 min min NN cord-319626-f1b3ygg0 74 14 at at IN cord-319626-f1b3ygg0 74 15 4 4 CD cord-319626-f1b3ygg0 74 16 ° ° NN cord-319626-f1b3ygg0 74 17 C c NN cord-319626-f1b3ygg0 74 18 . . . cord-319626-f1b3ygg0 75 1 The the DT cord-319626-f1b3ygg0 75 2 detergent detergent NN cord-319626-f1b3ygg0 75 3 phase phase NN cord-319626-f1b3ygg0 75 4 , , , cord-319626-f1b3ygg0 75 5 enriched enrich VBN cord-319626-f1b3ygg0 75 6 in in IN cord-319626-f1b3ygg0 75 7 the the DT cord-319626-f1b3ygg0 75 8 El El NNP cord-319626-f1b3ygg0 75 9 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 75 10 , , , cord-319626-f1b3ygg0 75 11 was be VBD cord-319626-f1b3ygg0 75 12 isolated isolate VBN cord-319626-f1b3ygg0 75 13 from from IN cord-319626-f1b3ygg0 75 14 the the DT cord-319626-f1b3ygg0 75 15 detergent detergent NN cord-319626-f1b3ygg0 75 16 - - HYPH cord-319626-f1b3ygg0 75 17 soluble soluble JJ cord-319626-f1b3ygg0 75 18 extract extract NN cord-319626-f1b3ygg0 75 19 as as IN cord-319626-f1b3ygg0 75 20 described describe VBN cord-319626-f1b3ygg0 75 21 by by IN cord-319626-f1b3ygg0 75 22 Bordier Bordier NNP cord-319626-f1b3ygg0 75 23 ( ( -LRB- cord-319626-f1b3ygg0 75 24 1981 1981 CD cord-319626-f1b3ygg0 75 25 ) ) -RRB- cord-319626-f1b3ygg0 75 26 . . . cord-319626-f1b3ygg0 76 1 The the DT cord-319626-f1b3ygg0 76 2 medium medium NN cord-319626-f1b3ygg0 76 3 containing contain VBG cord-319626-f1b3ygg0 76 4 labeled label VBN cord-319626-f1b3ygg0 76 5 virions virion NNS cord-319626-f1b3ygg0 76 6 was be VBD cord-319626-f1b3ygg0 76 7 collected collect VBN cord-319626-f1b3ygg0 76 8 and and CC cord-319626-f1b3ygg0 76 9 layered layer VBN cord-319626-f1b3ygg0 76 10 onto onto IN cord-319626-f1b3ygg0 76 11 a a DT cord-319626-f1b3ygg0 76 12 20 20 CD cord-319626-f1b3ygg0 76 13 - - SYM cord-319626-f1b3ygg0 76 14 50 50 CD cord-319626-f1b3ygg0 76 15 % % NN cord-319626-f1b3ygg0 76 16 continuous continuous JJ cord-319626-f1b3ygg0 76 17 sucrose sucrose NN cord-319626-f1b3ygg0 76 18 gradient gradient NN cord-319626-f1b3ygg0 76 19 as as IN cord-319626-f1b3ygg0 76 20 described describe VBN cord-319626-f1b3ygg0 76 21 by by IN cord-319626-f1b3ygg0 76 22 Spaan Spaan NNP cord-319626-f1b3ygg0 76 23 et et FW cord-319626-f1b3ygg0 76 24 al al NNP cord-319626-f1b3ygg0 76 25 . . . cord-319626-f1b3ygg0 77 1 ( ( -LRB- cord-319626-f1b3ygg0 77 2 1981 1981 CD cord-319626-f1b3ygg0 77 3 ) ) -RRB- cord-319626-f1b3ygg0 77 4 . . . cord-319626-f1b3ygg0 78 1 The the DT cord-319626-f1b3ygg0 78 2 virions virion NNS cord-319626-f1b3ygg0 78 3 were be VBD cord-319626-f1b3ygg0 78 4 harvested harvest VBN cord-319626-f1b3ygg0 78 5 from from IN cord-319626-f1b3ygg0 78 6 the the DT cord-319626-f1b3ygg0 78 7 gradients gradient NNS cord-319626-f1b3ygg0 78 8 after after IN cord-319626-f1b3ygg0 78 9 centrifugation centrifugation NN cord-319626-f1b3ygg0 78 10 for for IN cord-319626-f1b3ygg0 78 11 3 3 CD cord-319626-f1b3ygg0 78 12 h h NN cord-319626-f1b3ygg0 78 13 at at IN cord-319626-f1b3ygg0 78 14 100,000 100,000 CD cord-319626-f1b3ygg0 78 15 ga~. ga~. XX cord-319626-f1b3ygg0 79 1 The the DT cord-319626-f1b3ygg0 79 2 El El NNP cord-319626-f1b3ygg0 79 3 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 79 4 was be VBD cord-319626-f1b3ygg0 79 5 extracted extract VBN cord-319626-f1b3ygg0 79 6 from from IN cord-319626-f1b3ygg0 79 7 virions virion NNS cord-319626-f1b3ygg0 79 8 using use VBG cord-319626-f1b3ygg0 79 9 Tx Tx NNP cord-319626-f1b3ygg0 79 10 - - HYPH cord-319626-f1b3ygg0 79 11 ll4 ll4 NNP cord-319626-f1b3ygg0 79 12 as as IN cord-319626-f1b3ygg0 79 13 detailed detail VBN cord-319626-f1b3ygg0 79 14 above above RB cord-319626-f1b3ygg0 79 15 . . . cord-319626-f1b3ygg0 80 1 The the DT cord-319626-f1b3ygg0 80 2 El El NNP cord-319626-f1b3ygg0 80 3 - - HYPH cord-319626-f1b3ygg0 80 4 enriched enrich VBN cord-319626-f1b3ygg0 80 5 detergent detergent NN cord-319626-f1b3ygg0 80 6 extracts extract NNS cord-319626-f1b3ygg0 80 7 from from IN cord-319626-f1b3ygg0 80 8 cells cell NNS cord-319626-f1b3ygg0 80 9 or or CC cord-319626-f1b3ygg0 80 10 virus virus NN cord-319626-f1b3ygg0 80 11 were be VBD cord-319626-f1b3ygg0 80 12 solubilized solubilize VBN cord-319626-f1b3ygg0 80 13 and and CC cord-319626-f1b3ygg0 80 14 reduced reduce VBN cord-319626-f1b3ygg0 80 15 in in IN cord-319626-f1b3ygg0 80 16 sample sample NN cord-319626-f1b3ygg0 80 17 buffer buffer NN cord-319626-f1b3ygg0 80 18 , , , cord-319626-f1b3ygg0 80 19 alkylated alkylate VBN cord-319626-f1b3ygg0 80 20 ( ( -LRB- cord-319626-f1b3ygg0 80 21 Green Green NNP cord-319626-f1b3ygg0 80 22 et et FW cord-319626-f1b3ygg0 80 23 al al NNP cord-319626-f1b3ygg0 80 24 . . NNP cord-319626-f1b3ygg0 80 25 , , , cord-319626-f1b3ygg0 80 26 1981 1981 CD cord-319626-f1b3ygg0 80 27 ) ) -RRB- cord-319626-f1b3ygg0 80 28 , , , cord-319626-f1b3ygg0 80 29 and and CC cord-319626-f1b3ygg0 80 30 then then RB cord-319626-f1b3ygg0 80 31 fractionated fractionate VBN cord-319626-f1b3ygg0 80 32 by by IN cord-319626-f1b3ygg0 80 33 SDS SDS NNP cord-319626-f1b3ygg0 80 34 - - HYPH cord-319626-f1b3ygg0 80 35 PAGE PAGE NNP cord-319626-f1b3ygg0 80 36 using use VBG cord-319626-f1b3ygg0 80 37 a a DT cord-319626-f1b3ygg0 80 38 15 15 CD cord-319626-f1b3ygg0 80 39 % % NN cord-319626-f1b3ygg0 80 40 running run VBG cord-319626-f1b3ygg0 80 41 gel gel NN cord-319626-f1b3ygg0 80 42 and and CC cord-319626-f1b3ygg0 80 43 a a DT cord-319626-f1b3ygg0 80 44 5 5 CD cord-319626-f1b3ygg0 80 45 % % NN cord-319626-f1b3ygg0 80 46 stacking stacking NN cord-319626-f1b3ygg0 80 47 gel gel NN cord-319626-f1b3ygg0 80 48 ( ( -LRB- cord-319626-f1b3ygg0 80 49 Maizel Maizel NNP cord-319626-f1b3ygg0 80 50 , , , cord-319626-f1b3ygg0 80 51 1971 1971 CD cord-319626-f1b3ygg0 80 52 ) ) -RRB- cord-319626-f1b3ygg0 80 53 . . . cord-319626-f1b3ygg0 81 1 Incorporation incorporation NN cord-319626-f1b3ygg0 81 2 of of IN cord-319626-f1b3ygg0 81 3 radioactive radioactive JJ cord-319626-f1b3ygg0 82 1 [ [ -LRB- cord-319626-f1b3ygg0 82 2 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 82 3 was be VBD cord-319626-f1b3ygg0 82 4 determined determine VBN cord-319626-f1b3ygg0 82 5 after after IN cord-319626-f1b3ygg0 82 6 TCA TCA NNP cord-319626-f1b3ygg0 82 7 precipitation precipitation NN cord-319626-f1b3ygg0 82 8 ( ( -LRB- cord-319626-f1b3ygg0 82 9 Mans Mans NNP cord-319626-f1b3ygg0 82 10 and and CC cord-319626-f1b3ygg0 82 11 Novelli Novelli NNP cord-319626-f1b3ygg0 82 12 , , , cord-319626-f1b3ygg0 82 13 1961 1961 CD cord-319626-f1b3ygg0 82 14 ) ) -RRB- cord-319626-f1b3ygg0 82 15 and and CC cord-319626-f1b3ygg0 82 16 equal equal JJ cord-319626-f1b3ygg0 82 17 counts count NNS cord-319626-f1b3ygg0 82 18 were be VBD cord-319626-f1b3ygg0 82 19 loaded load VBN cord-319626-f1b3ygg0 82 20 onto onto IN cord-319626-f1b3ygg0 82 21 each each DT cord-319626-f1b3ygg0 82 22 lane lane NN cord-319626-f1b3ygg0 82 23 unless unless IN cord-319626-f1b3ygg0 82 24 otherwise otherwise RB cord-319626-f1b3ygg0 82 25 indicated indicate VBN cord-319626-f1b3ygg0 82 26 . . . cord-319626-f1b3ygg0 83 1 Gels gel NNS cord-319626-f1b3ygg0 83 2 were be VBD cord-319626-f1b3ygg0 83 3 fixed fix VBN cord-319626-f1b3ygg0 83 4 using use VBG cord-319626-f1b3ygg0 83 5 10 10 CD cord-319626-f1b3ygg0 83 6 % % NN cord-319626-f1b3ygg0 83 7 ( ( -LRB- cord-319626-f1b3ygg0 83 8 wt wt NN cord-319626-f1b3ygg0 83 9 / / SYM cord-319626-f1b3ygg0 83 10 vol vol NN cord-319626-f1b3ygg0 83 11 ) ) -RRB- cord-319626-f1b3ygg0 83 12 TCA TCA NNP cord-319626-f1b3ygg0 83 13 , , , cord-319626-f1b3ygg0 83 14 treated treat VBN cord-319626-f1b3ygg0 83 15 with with IN cord-319626-f1b3ygg0 83 16 EN3HANCE EN3HANCE NNP cord-319626-f1b3ygg0 83 17 , , , cord-319626-f1b3ygg0 83 18 dried dry VBN cord-319626-f1b3ygg0 83 19 , , , cord-319626-f1b3ygg0 83 20 and and CC cord-319626-f1b3ygg0 83 21 exposed expose VBN cord-319626-f1b3ygg0 83 22 to to IN cord-319626-f1b3ygg0 83 23 Kodak Kodak NNP cord-319626-f1b3ygg0 83 24 XS XS NNP cord-319626-f1b3ygg0 83 25 film film NN cord-319626-f1b3ygg0 83 26 for for IN cord-319626-f1b3ygg0 83 27 1 1 CD cord-319626-f1b3ygg0 83 28 - - SYM cord-319626-f1b3ygg0 83 29 5 5 CD cord-319626-f1b3ygg0 83 30 d d NN cord-319626-f1b3ygg0 83 31 at at IN cord-319626-f1b3ygg0 83 32 -80 -80 NNP cord-319626-f1b3ygg0 83 33 ° ° NN cord-319626-f1b3ygg0 83 34 C C NNP cord-319626-f1b3ygg0 83 35 . . . cord-319626-f1b3ygg0 84 1 To to TO cord-319626-f1b3ygg0 84 2 quantitate quantitate VB cord-319626-f1b3ygg0 84 3 the the DT cord-319626-f1b3ygg0 84 4 [ [ -LRB- cord-319626-f1b3ygg0 84 5 3~S]methionine 3~s]methionine CD cord-319626-f1b3ygg0 84 6 incorporated incorporate VBN cord-319626-f1b3ygg0 84 7 in in IN cord-319626-f1b3ygg0 84 8 the the DT cord-319626-f1b3ygg0 84 9 E1 e1 NN cord-319626-f1b3ygg0 84 10 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 84 11 the the DT cord-319626-f1b3ygg0 84 12 fluorogram fluorogram NN cord-319626-f1b3ygg0 84 13 was be VBD cord-319626-f1b3ygg0 84 14 exposed expose VBN cord-319626-f1b3ygg0 84 15 to to IN cord-319626-f1b3ygg0 84 16 Kodak Kodak NNP cord-319626-f1b3ygg0 84 17 X X NNP cord-319626-f1b3ygg0 84 18 - - NNP cord-319626-f1b3ygg0 84 19 AR5 AR5 NNP cord-319626-f1b3ygg0 84 20 film film NN cord-319626-f1b3ygg0 84 21 . . . cord-319626-f1b3ygg0 85 1 The the DT cord-319626-f1b3ygg0 85 2 fluorograph fluorograph NN cord-319626-f1b3ygg0 85 3 was be VBD cord-319626-f1b3ygg0 85 4 then then RB cord-319626-f1b3ygg0 85 5 used use VBN cord-319626-f1b3ygg0 85 6 to to TO cord-319626-f1b3ygg0 85 7 locate locate VB cord-319626-f1b3ygg0 85 8 the the DT cord-319626-f1b3ygg0 85 9 E1 e1 NN cord-319626-f1b3ygg0 85 10 bands band NNS cord-319626-f1b3ygg0 85 11 in in IN cord-319626-f1b3ygg0 85 12 the the DT cord-319626-f1b3ygg0 85 13 fluorogram fluorogram NN cord-319626-f1b3ygg0 85 14 . . . cord-319626-f1b3ygg0 86 1 The the DT cord-319626-f1b3ygg0 86 2 excised excise VBN cord-319626-f1b3ygg0 86 3 pieces piece NNS cord-319626-f1b3ygg0 86 4 were be VBD cord-319626-f1b3ygg0 86 5 rehydrated rehydrate VBN cord-319626-f1b3ygg0 86 6 and and CC cord-319626-f1b3ygg0 86 7 incubated incubate VBN cord-319626-f1b3ygg0 86 8 in in IN cord-319626-f1b3ygg0 86 9 0.5 0.5 CD cord-319626-f1b3ygg0 86 10 ml ml NNS cord-319626-f1b3ygg0 86 11 of of IN cord-319626-f1b3ygg0 86 12 30 30 CD cord-319626-f1b3ygg0 86 13 % % NN cord-319626-f1b3ygg0 86 14 ( ( -LRB- cord-319626-f1b3ygg0 86 15 vol vol NNP cord-319626-f1b3ygg0 86 16 / / SYM cord-319626-f1b3ygg0 86 17 vol vol NNP cord-319626-f1b3ygg0 86 18 ) ) -RRB- cord-319626-f1b3ygg0 86 19 H202 H202 NNP cord-319626-f1b3ygg0 86 20 in in IN cord-319626-f1b3ygg0 86 21 10 10 CD cord-319626-f1b3ygg0 86 22 mM mm CD cord-319626-f1b3ygg0 86 23 HC1 HC1 NNP cord-319626-f1b3ygg0 86 24 at at IN cord-319626-f1b3ygg0 86 25 60 60 CD cord-319626-f1b3ygg0 86 26 ° ° NN cord-319626-f1b3ygg0 86 27 C c NN cord-319626-f1b3ygg0 86 28 for for IN cord-319626-f1b3ygg0 86 29 48 48 CD cord-319626-f1b3ygg0 86 30 h. h. NNP cord-319626-f1b3ygg0 86 31 Scintillation Scintillation NNP cord-319626-f1b3ygg0 86 32 fluid fluid NN cord-319626-f1b3ygg0 86 33 ( ( -LRB- cord-319626-f1b3ygg0 86 34 10 10 CD cord-319626-f1b3ygg0 86 35 ml ml NN cord-319626-f1b3ygg0 86 36 ) ) -RRB- cord-319626-f1b3ygg0 86 37 ( ( -LRB- cord-319626-f1b3ygg0 86 38 Rotizant Rotizant NNP cord-319626-f1b3ygg0 86 39 22 22 CD cord-319626-f1b3ygg0 86 40 , , , cord-319626-f1b3ygg0 86 41 Firma Firma NNP cord-319626-f1b3ygg0 86 42 Roth Roth NNP cord-319626-f1b3ygg0 86 43 , , , cord-319626-f1b3ygg0 86 44 Karlsruhe Karlsruhe NNP cord-319626-f1b3ygg0 86 45 , , , cord-319626-f1b3ygg0 86 46 FRG FRG NNP cord-319626-f1b3ygg0 86 47 ) ) -RRB- cord-319626-f1b3ygg0 86 48 was be VBD cord-319626-f1b3ygg0 86 49 then then RB cord-319626-f1b3ygg0 86 50 added add VBN cord-319626-f1b3ygg0 86 51 and and CC cord-319626-f1b3ygg0 86 52 the the DT cord-319626-f1b3ygg0 86 53 samples sample NNS cord-319626-f1b3ygg0 86 54 were be VBD cord-319626-f1b3ygg0 86 55 cooled cool VBN cord-319626-f1b3ygg0 86 56 to to IN cord-319626-f1b3ygg0 86 57 4 4 CD cord-319626-f1b3ygg0 86 58 ° ° NN cord-319626-f1b3ygg0 86 59 C c NN cord-319626-f1b3ygg0 86 60 and and CC cord-319626-f1b3ygg0 86 61 counted count VBD cord-319626-f1b3ygg0 86 62 . . . cord-319626-f1b3ygg0 87 1 Gel gel NN cord-319626-f1b3ygg0 87 2 pieces piece NNS cord-319626-f1b3ygg0 87 3 of of IN cord-319626-f1b3ygg0 87 4 equivalent equivalent JJ cord-319626-f1b3ygg0 87 5 sizes size NNS cord-319626-f1b3ygg0 87 6 from from IN cord-319626-f1b3ygg0 87 7 areas area NNS cord-319626-f1b3ygg0 87 8 containing contain VBG cord-319626-f1b3ygg0 87 9 no no DT cord-319626-f1b3ygg0 87 10 samples sample NNS cord-319626-f1b3ygg0 87 11 were be VBD cord-319626-f1b3ygg0 87 12 treated treat VBN cord-319626-f1b3ygg0 87 13 similarly similarly RB cord-319626-f1b3ygg0 87 14 and and CC cord-319626-f1b3ygg0 87 15 used use VBN cord-319626-f1b3ygg0 87 16 to to TO cord-319626-f1b3ygg0 87 17 provide provide VB cord-319626-f1b3ygg0 87 18 blanks blank NNS cord-319626-f1b3ygg0 87 19 which which WDT cord-319626-f1b3ygg0 87 20 were be VBD cord-319626-f1b3ygg0 87 21 typically typically RB cord-319626-f1b3ygg0 87 22 < < XX cord-319626-f1b3ygg0 87 23 1 1 CD cord-319626-f1b3ygg0 87 24 % % NN cord-319626-f1b3ygg0 87 25 of of IN cord-319626-f1b3ygg0 87 26 the the DT cord-319626-f1b3ygg0 87 27 experimental experimental JJ cord-319626-f1b3ygg0 87 28 values value NNS cord-319626-f1b3ygg0 87 29 . . . cord-319626-f1b3ygg0 88 1 [ [ -LRB- cord-319626-f1b3ygg0 88 2 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 88 3 - - HYPH cord-319626-f1b3ygg0 88 4 labeled label VBN cord-319626-f1b3ygg0 88 5 cells cell NNS cord-319626-f1b3ygg0 88 6 were be VBD cord-319626-f1b3ygg0 88 7 solubilized solubilize VBN cord-319626-f1b3ygg0 88 8 in in IN cord-319626-f1b3ygg0 88 9 RIPA RIPA NNP cord-319626-f1b3ygg0 88 10 buffer buffer NN cord-319626-f1b3ygg0 88 11 ( ( -LRB- cord-319626-f1b3ygg0 88 12 10 10 CD cord-319626-f1b3ygg0 88 13 mM mM NNP cord-319626-f1b3ygg0 88 14 Tris Tris NNP cord-319626-f1b3ygg0 88 15 - - HYPH cord-319626-f1b3ygg0 88 16 HCI HCI NNP cord-319626-f1b3ygg0 88 17 , , , cord-319626-f1b3ygg0 88 18 pH pH NNP cord-319626-f1b3ygg0 88 19 7.6 7.6 CD cord-319626-f1b3ygg0 88 20 , , , cord-319626-f1b3ygg0 88 21 0.15 0.15 CD cord-319626-f1b3ygg0 88 22 M M NNP cord-319626-f1b3ygg0 88 23 NaCI NaCI NNP cord-319626-f1b3ygg0 88 24 , , , cord-319626-f1b3ygg0 88 25 1 1 CD cord-319626-f1b3ygg0 88 26 % % NN cord-319626-f1b3ygg0 88 27 [ [ -LRB- cord-319626-f1b3ygg0 88 28 wt wt NN cord-319626-f1b3ygg0 88 29 / / SYM cord-319626-f1b3ygg0 88 30 vol vol NN cord-319626-f1b3ygg0 88 31 ] ] -RRB- cord-319626-f1b3ygg0 88 32 Triton Triton NNP cord-319626-f1b3ygg0 88 33 X-100 x-100 NN cord-319626-f1b3ygg0 88 34 , , , cord-319626-f1b3ygg0 88 35 0.1 0.1 CD cord-319626-f1b3ygg0 88 36 % % NN cord-319626-f1b3ygg0 88 37 [ [ -LRB- cord-319626-f1b3ygg0 88 38 wt wt NN cord-319626-f1b3ygg0 88 39 / / SYM cord-319626-f1b3ygg0 88 40 vol vol NNP cord-319626-f1b3ygg0 88 41 ] ] -RRB- cord-319626-f1b3ygg0 88 42 SDS SDS NNP cord-319626-f1b3ygg0 88 43 , , , cord-319626-f1b3ygg0 88 44 and and CC cord-319626-f1b3ygg0 88 45 1 1 CD cord-319626-f1b3ygg0 88 46 % % NN cord-319626-f1b3ygg0 88 47 [ [ -LRB- cord-319626-f1b3ygg0 88 48 wt wt NN cord-319626-f1b3ygg0 88 49 / / SYM cord-319626-f1b3ygg0 88 50 vol vol NN cord-319626-f1b3ygg0 88 51 ] ] -RRB- cord-319626-f1b3ygg0 88 52 sodium sodium NN cord-319626-f1b3ygg0 88 53 deoxycholate deoxycholate NNP cord-319626-f1b3ygg0 88 54 ) ) -RRB- cord-319626-f1b3ygg0 88 55 containing contain VBG cord-319626-f1b3ygg0 88 56 a a DT cord-319626-f1b3ygg0 88 57 cocktail cocktail NN cord-319626-f1b3ygg0 88 58 of of IN cord-319626-f1b3ygg0 88 59 protease protease NN cord-319626-f1b3ygg0 88 60 inhibitors inhibitor NNS cord-319626-f1b3ygg0 88 61 ( ( -LRB- cord-319626-f1b3ygg0 88 62 40 40 CD cord-319626-f1b3ygg0 88 63 ~tg ~tg NNS cord-319626-f1b3ygg0 88 64 / / SYM cord-319626-f1b3ygg0 88 65 ml ml IN cord-319626-f1b3ygg0 88 66 PMSF PMSF NNP cord-319626-f1b3ygg0 88 67 , , , cord-319626-f1b3ygg0 88 68 1 1 CD cord-319626-f1b3ygg0 88 69 lag lag NN cord-319626-f1b3ygg0 88 70 / / SYM cord-319626-f1b3ygg0 88 71 ml ml NN cord-319626-f1b3ygg0 88 72 leupeptin leupeptin NN cord-319626-f1b3ygg0 88 73 , , , cord-319626-f1b3ygg0 89 1 1 1 CD cord-319626-f1b3ygg0 89 2 Ixg Ixg NNP cord-319626-f1b3ygg0 89 3 / / SYM cord-319626-f1b3ygg0 89 4 ml ml NN cord-319626-f1b3ygg0 89 5 antipain antipain NNP cord-319626-f1b3ygg0 89 6 , , , cord-319626-f1b3ygg0 89 7 1 1 CD cord-319626-f1b3ygg0 89 8 mM mm CD cord-319626-f1b3ygg0 89 9 benzamidine benzamidine NN cord-319626-f1b3ygg0 89 10 , , , cord-319626-f1b3ygg0 89 11 and and CC cord-319626-f1b3ygg0 89 12 10 10 CD cord-319626-f1b3ygg0 89 13 U u NN cord-319626-f1b3ygg0 89 14 / / SYM cord-319626-f1b3ygg0 89 15 ml ml NN cord-319626-f1b3ygg0 89 16 Trasylol Trasylol NNP cord-319626-f1b3ygg0 89 17 ) ) -RRB- cord-319626-f1b3ygg0 89 18 . . . cord-319626-f1b3ygg0 90 1 The the DT cord-319626-f1b3ygg0 90 2 extract extract NN cord-319626-f1b3ygg0 90 3 was be VBD cord-319626-f1b3ygg0 90 4 centrifuged centrifuge VBN cord-319626-f1b3ygg0 90 5 at at IN cord-319626-f1b3ygg0 90 6 10,000 10,000 CD cord-319626-f1b3ygg0 90 7 ga~ ga~ NNP cord-319626-f1b3ygg0 90 8 and and CC cord-319626-f1b3ygg0 90 9 the the DT cord-319626-f1b3ygg0 90 10 supernatant supernatant NN cord-319626-f1b3ygg0 90 11 was be VBD cord-319626-f1b3ygg0 90 12 treated treat VBN cord-319626-f1b3ygg0 90 13 with with IN cord-319626-f1b3ygg0 90 14 5 5 CD cord-319626-f1b3ygg0 90 15 111 111 CD cord-319626-f1b3ygg0 90 16 of of IN cord-319626-f1b3ygg0 90 17 a a DT cord-319626-f1b3ygg0 90 18 polyclonal polyclonal JJ cord-319626-f1b3ygg0 90 19 antiserum antiserum NN cord-319626-f1b3ygg0 90 20 raised raise VBN cord-319626-f1b3ygg0 90 21 against against IN cord-319626-f1b3ygg0 90 22 a a DT cord-319626-f1b3ygg0 90 23 Triton Triton NNP cord-319626-f1b3ygg0 90 24 X-114 X-114 VBZ cord-319626-f1b3ygg0 90 25 extract extract NN cord-319626-f1b3ygg0 90 26 of of IN cord-319626-f1b3ygg0 90 27 gradient gradient NN cord-319626-f1b3ygg0 90 28 - - HYPH cord-319626-f1b3ygg0 90 29 purified purify VBN cord-319626-f1b3ygg0 90 30 virions virion NNS cord-319626-f1b3ygg0 90 31 ( ( -LRB- cord-319626-f1b3ygg0 90 32 Tooze Tooze NNP cord-319626-f1b3ygg0 90 33 and and CC cord-319626-f1b3ygg0 90 34 Stanley Stanley NNP cord-319626-f1b3ygg0 90 35 , , , cord-319626-f1b3ygg0 90 36 1986 1986 CD cord-319626-f1b3ygg0 90 37 ) ) -RRB- cord-319626-f1b3ygg0 90 38 . . . cord-319626-f1b3ygg0 91 1 After after IN cord-319626-f1b3ygg0 91 2 overnight overnight JJ cord-319626-f1b3ygg0 91 3 incubation incubation NN cord-319626-f1b3ygg0 91 4 at at IN cord-319626-f1b3ygg0 91 5 4 4 CD cord-319626-f1b3ygg0 91 6 ° ° NN cord-319626-f1b3ygg0 91 7 C c NN cord-319626-f1b3ygg0 91 8 , , , cord-319626-f1b3ygg0 91 9 the the DT cord-319626-f1b3ygg0 91 10 immune immune JJ cord-319626-f1b3ygg0 91 11 complexes complex NNS cord-319626-f1b3ygg0 91 12 were be VBD cord-319626-f1b3ygg0 91 13 immobilized immobilize VBN cord-319626-f1b3ygg0 91 14 on on IN cord-319626-f1b3ygg0 91 15 Pansorbin Pansorbin NNP cord-319626-f1b3ygg0 91 16 for for IN cord-319626-f1b3ygg0 91 17 1 1 CD cord-319626-f1b3ygg0 91 18 h h NN cord-319626-f1b3ygg0 91 19 at at IN cord-319626-f1b3ygg0 91 20 37 37 CD cord-319626-f1b3ygg0 91 21 ° ° NN cord-319626-f1b3ygg0 91 22 C c NN cord-319626-f1b3ygg0 91 23 and and CC cord-319626-f1b3ygg0 91 24 washed wash VBD cord-319626-f1b3ygg0 91 25 three three CD cord-319626-f1b3ygg0 91 26 times time NNS cord-319626-f1b3ygg0 91 27 with with IN cord-319626-f1b3ygg0 91 28 RIPA RIPA NNP cord-319626-f1b3ygg0 91 29 buffer buffer NN cord-319626-f1b3ygg0 91 30 . . . cord-319626-f1b3ygg0 92 1 One one CD cord-319626-f1b3ygg0 92 2 half half NN cord-319626-f1b3ygg0 92 3 of of IN cord-319626-f1b3ygg0 92 4 the the DT cord-319626-f1b3ygg0 92 5 sample sample NN cord-319626-f1b3ygg0 92 6 was be VBD cord-319626-f1b3ygg0 92 7 then then RB cord-319626-f1b3ygg0 92 8 digested digest VBN cord-319626-f1b3ygg0 92 9 with with IN cord-319626-f1b3ygg0 92 10 1 1 CD cord-319626-f1b3ygg0 92 11 mU mU NNS cord-319626-f1b3ygg0 92 12 / / SYM cord-319626-f1b3ygg0 92 13 ml ml NN cord-319626-f1b3ygg0 92 14 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 92 15 from from IN cord-319626-f1b3ygg0 92 16 Clostridium clostridium NN cord-319626-f1b3ygg0 92 17 perfringens perfringen NNS cord-319626-f1b3ygg0 92 18 in in IN cord-319626-f1b3ygg0 92 19 10 10 CD cord-319626-f1b3ygg0 92 20 mM mm CD cord-319626-f1b3ygg0 92 21 sodium sodium NN cord-319626-f1b3ygg0 92 22 acetate acetate NN cord-319626-f1b3ygg0 92 23 buffer buffer NN cord-319626-f1b3ygg0 92 24 , , , cord-319626-f1b3ygg0 92 25 pH pH NNP cord-319626-f1b3ygg0 92 26 5.6 5.6 CD cord-319626-f1b3ygg0 92 27 , , , cord-319626-f1b3ygg0 92 28 conmining conmine VBG cord-319626-f1b3ygg0 92 29 1 1 CD cord-319626-f1b3ygg0 92 30 mM mm CD cord-319626-f1b3ygg0 92 31 CaC12 CaC12 NNP cord-319626-f1b3ygg0 92 32 , , , cord-319626-f1b3ygg0 92 33 for for IN cord-319626-f1b3ygg0 92 34 16 16 CD cord-319626-f1b3ygg0 92 35 h h NN cord-319626-f1b3ygg0 92 36 at at IN cord-319626-f1b3ygg0 92 37 37 37 CD cord-319626-f1b3ygg0 92 38 ° ° NN cord-319626-f1b3ygg0 92 39 C c NN cord-319626-f1b3ygg0 92 40 . . . cord-319626-f1b3ygg0 93 1 The the DT cord-319626-f1b3ygg0 93 2 digested digest VBN cord-319626-f1b3ygg0 93 3 immune immune JJ cord-319626-f1b3ygg0 93 4 complexes complex NNS cord-319626-f1b3ygg0 93 5 were be VBD cord-319626-f1b3ygg0 93 6 then then RB cord-319626-f1b3ygg0 93 7 washed wash VBN cord-319626-f1b3ygg0 93 8 well well RB cord-319626-f1b3ygg0 93 9 with with IN cord-319626-f1b3ygg0 93 10 10 10 CD cord-319626-f1b3ygg0 93 11 mM mM NNP cord-319626-f1b3ygg0 93 12 Tris Tris NNP cord-319626-f1b3ygg0 93 13 - - HYPH cord-319626-f1b3ygg0 93 14 HCl HCl NNP cord-319626-f1b3ygg0 93 15 buffer buffer NN cord-319626-f1b3ygg0 93 16 , , , cord-319626-f1b3ygg0 93 17 pH pH NNP cord-319626-f1b3ygg0 93 18 7.2 7.2 CD cord-319626-f1b3ygg0 93 19 , , , cord-319626-f1b3ygg0 93 20 containing contain VBG cord-319626-f1b3ygg0 93 21 the the DT cord-319626-f1b3ygg0 93 22 protease protease NN cord-319626-f1b3ygg0 93 23 inhibitor inhibitor NN cord-319626-f1b3ygg0 93 24 cocktail cocktail NN cord-319626-f1b3ygg0 93 25 . . . cord-319626-f1b3ygg0 94 1 These these DT cord-319626-f1b3ygg0 94 2 samples sample NNS cord-319626-f1b3ygg0 94 3 , , , cord-319626-f1b3ygg0 94 4 together together RB cord-319626-f1b3ygg0 94 5 with with IN cord-319626-f1b3ygg0 94 6 the the DT cord-319626-f1b3ygg0 94 7 untreated untreated JJ cord-319626-f1b3ygg0 94 8 ones one NNS cord-319626-f1b3ygg0 94 9 , , , cord-319626-f1b3ygg0 94 10 were be VBD cord-319626-f1b3ygg0 94 11 then then RB cord-319626-f1b3ygg0 94 12 prepared prepare VBN cord-319626-f1b3ygg0 94 13 for for IN cord-319626-f1b3ygg0 94 14 and and CC cord-319626-f1b3ygg0 94 15 fractionated fractionate VBN cord-319626-f1b3ygg0 94 16 by by IN cord-319626-f1b3ygg0 94 17 SDS SDS NNP cord-319626-f1b3ygg0 94 18 - - HYPH cord-319626-f1b3ygg0 94 19 PAGE PAGE NNP cord-319626-f1b3ygg0 94 20 . . . cord-319626-f1b3ygg0 95 1 The the DT cord-319626-f1b3ygg0 95 2 growth growth NN cord-319626-f1b3ygg0 95 3 medium medium NN cord-319626-f1b3ygg0 95 4 was be VBD cord-319626-f1b3ygg0 95 5 changed change VBN cord-319626-f1b3ygg0 95 6 at at IN cord-319626-f1b3ygg0 95 7 6 6 CD cord-319626-f1b3ygg0 95 8 h h NN cord-319626-f1b3ygg0 95 9 after after IN cord-319626-f1b3ygg0 95 10 infection infection NN cord-319626-f1b3ygg0 95 11 and and CC cord-319626-f1b3ygg0 95 12 cultures culture NNS cord-319626-f1b3ygg0 95 13 were be VBD cord-319626-f1b3ygg0 95 14 either either CC cord-319626-f1b3ygg0 95 15 left leave VBN cord-319626-f1b3ygg0 95 16 at at IN cord-319626-f1b3ygg0 95 17 37 37 CD cord-319626-f1b3ygg0 95 18 ° ° NN cord-319626-f1b3ygg0 95 19 C c NN cord-319626-f1b3ygg0 95 20 or or CC cord-319626-f1b3ygg0 95 21 shifted shift VBD cord-319626-f1b3ygg0 95 22 to to IN cord-319626-f1b3ygg0 95 23 31 31 CD cord-319626-f1b3ygg0 95 24 ° ° NN cord-319626-f1b3ygg0 95 25 C c NN cord-319626-f1b3ygg0 95 26 for for IN cord-319626-f1b3ygg0 95 27 the the DT cord-319626-f1b3ygg0 95 28 time time NN cord-319626-f1b3ygg0 95 29 period period NN cord-319626-f1b3ygg0 95 30 indicated indicate VBD cord-319626-f1b3ygg0 95 31 . . . cord-319626-f1b3ygg0 96 1 The the DT cord-319626-f1b3ygg0 96 2 cultures culture NNS cord-319626-f1b3ygg0 96 3 were be VBD cord-319626-f1b3ygg0 96 4 treated treat VBN cord-319626-f1b3ygg0 96 5 at at IN cord-319626-f1b3ygg0 96 6 3.5 3.5 CD cord-319626-f1b3ygg0 96 7 h h NN cord-319626-f1b3ygg0 96 8 after after IN cord-319626-f1b3ygg0 96 9 infection infection NN cord-319626-f1b3ygg0 96 10 with with IN cord-319626-f1b3ygg0 96 11 proteinase proteinase NN cord-319626-f1b3ygg0 96 12 K K NNP cord-319626-f1b3ygg0 96 13 to to TO cord-319626-f1b3ygg0 96 14 remove remove VB cord-319626-f1b3ygg0 96 15 any any DT cord-319626-f1b3ygg0 96 16 virions virion NNS cord-319626-f1b3ygg0 96 17 from from IN cord-319626-f1b3ygg0 96 18 the the DT cord-319626-f1b3ygg0 96 19 infecting infect VBG cord-319626-f1b3ygg0 96 20 stock stock NN cord-319626-f1b3ygg0 96 21 bound bind VBN cord-319626-f1b3ygg0 96 22 to to IN cord-319626-f1b3ygg0 96 23 the the DT cord-319626-f1b3ygg0 96 24 surface surface NN cord-319626-f1b3ygg0 96 25 . . . cord-319626-f1b3ygg0 97 1 * * NFP cord-319626-f1b3ygg0 97 2 Temperature temperature NN cord-319626-f1b3ygg0 97 3 of of IN cord-319626-f1b3ygg0 97 4 incubation incubation NN cord-319626-f1b3ygg0 97 5 . . . cord-319626-f1b3ygg0 98 1 nd nd NNP cord-319626-f1b3ygg0 98 2 , , , cord-319626-f1b3ygg0 98 3 none none NN cord-319626-f1b3ygg0 98 4 detectable detectable JJ cord-319626-f1b3ygg0 98 5 in in IN cord-319626-f1b3ygg0 98 6 undiluted undiluted JJ cord-319626-f1b3ygg0 98 7 supernatant supernatant NN cord-319626-f1b3ygg0 98 8 . . . cord-319626-f1b3ygg0 99 1 Infected infected JJ cord-319626-f1b3ygg0 99 2 sac(- sac(- NNP cord-319626-f1b3ygg0 99 3 ) ) -RRB- cord-319626-f1b3ygg0 99 4 cells cell NNS cord-319626-f1b3ygg0 99 5 ( ( -LRB- cord-319626-f1b3ygg0 99 6 2 2 CD cord-319626-f1b3ygg0 99 7 × × NN cord-319626-f1b3ygg0 99 8 108 108 CD cord-319626-f1b3ygg0 99 9 cells cell NNS cord-319626-f1b3ygg0 99 10 ) ) -RRB- cord-319626-f1b3ygg0 99 11 were be VBD cord-319626-f1b3ygg0 99 12 labeled label VBN cord-319626-f1b3ygg0 99 13 for for IN cord-319626-f1b3ygg0 99 14 12 12 CD cord-319626-f1b3ygg0 99 15 h h NN cord-319626-f1b3ygg0 99 16 with with IN cord-319626-f1b3ygg0 99 17 25 25 CD cord-319626-f1b3ygg0 99 18 laCi laCi NNS cord-319626-f1b3ygg0 99 19 / / SYM cord-319626-f1b3ygg0 99 20 ml ml NN cord-319626-f1b3ygg0 99 21 [ [ -LRB- cord-319626-f1b3ygg0 99 22 1,6 1,6 CD cord-319626-f1b3ygg0 99 23 - - HYPH cord-319626-f1b3ygg0 99 24 3H]glucosamine 3h]glucosamine CD cord-319626-f1b3ygg0 100 1 in in IN cord-319626-f1b3ygg0 100 2 MEM MEM NNP cord-319626-f1b3ygg0 100 3 containing contain VBG cord-319626-f1b3ygg0 100 4 10 10 CD cord-319626-f1b3ygg0 100 5 % % NN cord-319626-f1b3ygg0 100 6 the the DT cord-319626-f1b3ygg0 100 7 normal normal JJ cord-319626-f1b3ygg0 100 8 concentration concentration NN cord-319626-f1b3ygg0 100 9 of of IN cord-319626-f1b3ygg0 100 10 glucose glucose NN cord-319626-f1b3ygg0 100 11 ( ( -LRB- cord-319626-f1b3ygg0 100 12 100 100 CD cord-319626-f1b3ygg0 100 13 I.tg/ml i.tg/ml CD cord-319626-f1b3ygg0 100 14 ) ) -RRB- cord-319626-f1b3ygg0 100 15 , , , cord-319626-f1b3ygg0 100 16 10 10 CD cord-319626-f1b3ygg0 100 17 % % NN cord-319626-f1b3ygg0 100 18 ( ( -LRB- cord-319626-f1b3ygg0 100 19 vol vol NNP cord-319626-f1b3ygg0 100 20 / / SYM cord-319626-f1b3ygg0 100 21 vol vol NNP cord-319626-f1b3ygg0 100 22 ) ) -RRB- cord-319626-f1b3ygg0 100 23 dialyzed dialyze VBD cord-319626-f1b3ygg0 100 24 FCS FCS NNP cord-319626-f1b3ygg0 100 25 , , , cord-319626-f1b3ygg0 100 26 10 10 CD cord-319626-f1b3ygg0 100 27 mM mM NNP cord-319626-f1b3ygg0 100 28 Hepes Hepes NNP cord-319626-f1b3ygg0 100 29 buffer buffer NN cord-319626-f1b3ygg0 100 30 , , , cord-319626-f1b3ygg0 100 31 pH pH NNP cord-319626-f1b3ygg0 100 32 Z2 Z2 NNP cord-319626-f1b3ygg0 100 33 , , , cord-319626-f1b3ygg0 100 34 10 10 CD cord-319626-f1b3ygg0 100 35 mM mm CD cord-319626-f1b3ygg0 100 36 sodium sodium NN cord-319626-f1b3ygg0 100 37 pyruvate pyruvate NN cord-319626-f1b3ygg0 100 38 , , , cord-319626-f1b3ygg0 100 39 and and CC cord-319626-f1b3ygg0 100 40 100 100 CD cord-319626-f1b3ygg0 100 41 Ixg Ixg NNP cord-319626-f1b3ygg0 100 42 / / SYM cord-319626-f1b3ygg0 100 43 ml ml XX cord-319626-f1b3ygg0 100 44 each each DT cord-319626-f1b3ygg0 100 45 of of IN cord-319626-f1b3ygg0 100 46 penicillin penicillin NN cord-319626-f1b3ygg0 100 47 and and CC cord-319626-f1b3ygg0 100 48 streptomycin streptomycin NNS cord-319626-f1b3ygg0 100 49 . . . cord-319626-f1b3ygg0 101 1 The the DT cord-319626-f1b3ygg0 101 2 cells cell NNS cord-319626-f1b3ygg0 101 3 were be VBD cord-319626-f1b3ygg0 101 4 solubilized solubilize VBN cord-319626-f1b3ygg0 101 5 in in IN cord-319626-f1b3ygg0 101 6 RIPA RIPA NNP cord-319626-f1b3ygg0 101 7 buffer buffer NN cord-319626-f1b3ygg0 101 8 and and CC cord-319626-f1b3ygg0 101 9 the the DT cord-319626-f1b3ygg0 101 10 El El NNP cord-319626-f1b3ygg0 101 11 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 101 12 was be VBD cord-319626-f1b3ygg0 101 13 immunoprecipitated immunoprecipitate VBN cord-319626-f1b3ygg0 101 14 as as IN cord-319626-f1b3ygg0 101 15 described describe VBN cord-319626-f1b3ygg0 101 16 above above RB cord-319626-f1b3ygg0 101 17 . . . cord-319626-f1b3ygg0 102 1 The the DT cord-319626-f1b3ygg0 102 2 procedures procedure NNS cord-319626-f1b3ygg0 102 3 described describe VBN cord-319626-f1b3ygg0 102 4 below below RB cord-319626-f1b3ygg0 102 5 are be VBP cord-319626-f1b3ygg0 102 6 adapted adapt VBN cord-319626-f1b3ygg0 102 7 from from IN cord-319626-f1b3ygg0 102 8 Cummings Cummings NNP cord-319626-f1b3ygg0 102 9 et et FW cord-319626-f1b3ygg0 102 10 al al NNP cord-319626-f1b3ygg0 102 11 . . . cord-319626-f1b3ygg0 103 1 ( ( -LRB- cord-319626-f1b3ygg0 103 2 1983 1983 CD cord-319626-f1b3ygg0 103 3 ) ) -RRB- cord-319626-f1b3ygg0 103 4 . . . cord-319626-f1b3ygg0 104 1 After after IN cord-319626-f1b3ygg0 104 2 SDS SDS NNP cord-319626-f1b3ygg0 104 3 - - HYPH cord-319626-f1b3ygg0 104 4 PAGE PAGE NNP cord-319626-f1b3ygg0 104 5 the the DT cord-319626-f1b3ygg0 104 6 individual individual JJ cord-319626-f1b3ygg0 104 7 glycosylated glycosylated JJ cord-319626-f1b3ygg0 104 8 forms form NNS cord-319626-f1b3ygg0 104 9 of of IN cord-319626-f1b3ygg0 104 10 E1 e1 NN cord-319626-f1b3ygg0 104 11 were be VBD cord-319626-f1b3ygg0 104 12 excised excise VBN cord-319626-f1b3ygg0 104 13 from from IN cord-319626-f1b3ygg0 104 14 the the DT cord-319626-f1b3ygg0 104 15 dried dry VBN cord-319626-f1b3ygg0 104 16 gel gel NN cord-319626-f1b3ygg0 104 17 , , , cord-319626-f1b3ygg0 104 18 rehydrated rehydrate VBN cord-319626-f1b3ygg0 104 19 , , , cord-319626-f1b3ygg0 104 20 and and CC cord-319626-f1b3ygg0 104 21 digested digest VBN cord-319626-f1b3ygg0 104 22 with with IN cord-319626-f1b3ygg0 104 23 pronase pronase NNP cord-319626-f1b3ygg0 104 24 ( ( -LRB- cord-319626-f1b3ygg0 104 25 final final JJ cord-319626-f1b3ygg0 104 26 concentration concentration NN cord-319626-f1b3ygg0 104 27 5 5 CD cord-319626-f1b3ygg0 104 28 mg mg NNP cord-319626-f1b3ygg0 104 29 / / SYM cord-319626-f1b3ygg0 104 30 ml ml NNP cord-319626-f1b3ygg0 104 31 ) ) -RRB- cord-319626-f1b3ygg0 104 32 . . . cord-319626-f1b3ygg0 105 1 The the DT cord-319626-f1b3ygg0 105 2 glycopeptides glycopeptide NNS cord-319626-f1b3ygg0 105 3 were be VBD cord-319626-f1b3ygg0 105 4 eluted elute VBN cord-319626-f1b3ygg0 105 5 from from IN cord-319626-f1b3ygg0 105 6 the the DT cord-319626-f1b3ygg0 105 7 gel gel NN cord-319626-f1b3ygg0 105 8 pieces piece NNS cord-319626-f1b3ygg0 105 9 using use VBG cord-319626-f1b3ygg0 105 10 HzO. HzO. NNP cord-319626-f1b3ygg0 106 1 The the DT cord-319626-f1b3ygg0 106 2 eluate eluate NN cord-319626-f1b3ygg0 106 3 conmining conmine VBG cord-319626-f1b3ygg0 106 4 the the DT cord-319626-f1b3ygg0 106 5 glycopeptides glycopeptide NNS cord-319626-f1b3ygg0 106 6 was be VBD cord-319626-f1b3ygg0 106 7 then then RB cord-319626-f1b3ygg0 106 8 lyophilized lyophilize VBN cord-319626-f1b3ygg0 106 9 and and CC cord-319626-f1b3ygg0 106 10 resuspended resuspend VBD cord-319626-f1b3ygg0 106 11 in in IN cord-319626-f1b3ygg0 106 12 H20 H20 NNP cord-319626-f1b3ygg0 106 13 . . . cord-319626-f1b3ygg0 107 1 Glycopeptides glycopeptide NNS cord-319626-f1b3ygg0 107 2 were be VBD cord-319626-f1b3ygg0 107 3 acetylated acetylate VBN cord-319626-f1b3ygg0 107 4 by by IN cord-319626-f1b3ygg0 107 5 treatment treatment NN cord-319626-f1b3ygg0 107 6 with with IN cord-319626-f1b3ygg0 107 7 acetic acetic JJ cord-319626-f1b3ygg0 107 8 anhydride anhydride NN cord-319626-f1b3ygg0 108 1 ( ( -LRB- cord-319626-f1b3ygg0 108 2 Baenziger Baenziger NNP cord-319626-f1b3ygg0 108 3 and and CC cord-319626-f1b3ygg0 108 4 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 108 5 , , , cord-319626-f1b3ygg0 108 6 1974 1974 CD cord-319626-f1b3ygg0 108 7 ) ) -RRB- cord-319626-f1b3ygg0 108 8 and and CC cord-319626-f1b3ygg0 108 9 desalted desalt VBD cord-319626-f1b3ygg0 108 10 over over IN cord-319626-f1b3ygg0 108 11 an an DT cord-319626-f1b3ygg0 108 12 Amberlite Amberlite NNP cord-319626-f1b3ygg0 108 13 mixed mixed JJ cord-319626-f1b3ygg0 108 14 bed bed NN cord-319626-f1b3ygg0 108 15 resin resin NN cord-319626-f1b3ygg0 108 16 before before IN cord-319626-f1b3ygg0 108 17 mild mild JJ cord-319626-f1b3ygg0 108 18 alkaline alkaline JJ cord-319626-f1b3ygg0 108 19 hydrolysis hydrolysis NN cord-319626-f1b3ygg0 108 20 . . . cord-319626-f1b3ygg0 109 1 The the DT cord-319626-f1b3ygg0 109 2 eluate eluate NN cord-319626-f1b3ygg0 109 3 , , , cord-319626-f1b3ygg0 109 4 after after IN cord-319626-f1b3ygg0 109 5 lyophilization lyophilization NN cord-319626-f1b3ygg0 109 6 , , , cord-319626-f1b3ygg0 109 7 was be VBD cord-319626-f1b3ygg0 109 8 resuspended resuspend VBN cord-319626-f1b3ygg0 109 9 in in IN cord-319626-f1b3ygg0 109 10 0.2 0.2 CD cord-319626-f1b3ygg0 109 11 M m NN cord-319626-f1b3ygg0 109 12 sodium sodium NN cord-319626-f1b3ygg0 109 13 carbonate carbonate NN cord-319626-f1b3ygg0 109 14 , , , cord-319626-f1b3ygg0 109 15 pH pH NNP cord-319626-f1b3ygg0 109 16 11.6 11.6 CD cord-319626-f1b3ygg0 109 17 , , , cord-319626-f1b3ygg0 109 18 then then RB cord-319626-f1b3ygg0 109 19 treated treat VBN cord-319626-f1b3ygg0 109 20 with with IN cord-319626-f1b3ygg0 109 21 an an DT cord-319626-f1b3ygg0 109 22 equal equal JJ cord-319626-f1b3ygg0 109 23 volume volume NN cord-319626-f1b3ygg0 109 24 of of IN cord-319626-f1b3ygg0 109 25 2 2 CD cord-319626-f1b3ygg0 109 26 M M NNP cord-319626-f1b3ygg0 109 27 NaBH4 NaBH4 NNP cord-319626-f1b3ygg0 109 28 in in IN cord-319626-f1b3ygg0 109 29 100 100 CD cord-319626-f1b3ygg0 109 30 mM mm CD cord-319626-f1b3ygg0 109 31 NaOH NaOH NNP cord-319626-f1b3ygg0 109 32 , , , cord-319626-f1b3ygg0 109 33 and and CC cord-319626-f1b3ygg0 109 34 incubated incubate VBN cord-319626-f1b3ygg0 109 35 for for IN cord-319626-f1b3ygg0 109 36 16 16 CD cord-319626-f1b3ygg0 109 37 h h NN cord-319626-f1b3ygg0 109 38 at at IN cord-319626-f1b3ygg0 109 39 45 45 CD cord-319626-f1b3ygg0 109 40 ° ° NN cord-319626-f1b3ygg0 109 41 C c NN cord-319626-f1b3ygg0 109 42 as as IN cord-319626-f1b3ygg0 109 43 described describe VBN cord-319626-f1b3ygg0 109 44 by by IN cord-319626-f1b3ygg0 109 45 Niemann Niemann NNP cord-319626-f1b3ygg0 109 46 and and CC cord-319626-f1b3ygg0 109 47 Klenk Klenk NNP cord-319626-f1b3ygg0 109 48 ( ( -LRB- cord-319626-f1b3ygg0 109 49 1981 1981 CD cord-319626-f1b3ygg0 109 50 ) ) -RRB- cord-319626-f1b3ygg0 109 51 . . . cord-319626-f1b3ygg0 110 1 After after IN cord-319626-f1b3ygg0 110 2 neutralization neutralization NN cord-319626-f1b3ygg0 110 3 at at IN cord-319626-f1b3ygg0 110 4 4 4 CD cord-319626-f1b3ygg0 110 5 ° ° NN cord-319626-f1b3ygg0 110 6 C c NN cord-319626-f1b3ygg0 110 7 with with IN cord-319626-f1b3ygg0 110 8 glacial glacial JJ cord-319626-f1b3ygg0 110 9 acetic acetic JJ cord-319626-f1b3ygg0 110 10 acid acid NN cord-319626-f1b3ygg0 110 11 , , , cord-319626-f1b3ygg0 110 12 the the DT cord-319626-f1b3ygg0 110 13 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 110 14 were be VBD cord-319626-f1b3ygg0 110 15 passed pass VBN cord-319626-f1b3ygg0 110 16 over over RP cord-319626-f1b3ygg0 110 17 a a DT cord-319626-f1b3ygg0 110 18 Dowex Dowex NNP cord-319626-f1b3ygg0 110 19 50W 50w NN cord-319626-f1b3ygg0 110 20 - - HYPH cord-319626-f1b3ygg0 110 21 X8H x8h NN cord-319626-f1b3ygg0 110 22 + + CC cord-319626-f1b3ygg0 110 23 column column NN cord-319626-f1b3ygg0 110 24 and and CC cord-319626-f1b3ygg0 110 25 eluted elute VBD cord-319626-f1b3ygg0 110 26 with with IN cord-319626-f1b3ygg0 110 27 1 1 CD cord-319626-f1b3ygg0 110 28 % % NN cord-319626-f1b3ygg0 110 29 ( ( -LRB- cord-319626-f1b3ygg0 110 30 vol vol NNP cord-319626-f1b3ygg0 110 31 / / SYM cord-319626-f1b3ygg0 110 32 vol vol NN cord-319626-f1b3ygg0 110 33 ) ) -RRB- cord-319626-f1b3ygg0 110 34 acetic acetic JJ cord-319626-f1b3ygg0 110 35 acid acid NN cord-319626-f1b3ygg0 110 36 . . . cord-319626-f1b3ygg0 111 1 The the DT cord-319626-f1b3ygg0 111 2 peak peak NN cord-319626-f1b3ygg0 111 3 fractions fraction NNS cord-319626-f1b3ygg0 111 4 containing contain VBG cord-319626-f1b3ygg0 111 5 the the DT cord-319626-f1b3ygg0 111 6 oligosaccbarides oligosaccbaride NNS cord-319626-f1b3ygg0 111 7 were be VBD cord-319626-f1b3ygg0 111 8 pooled pool VBN cord-319626-f1b3ygg0 111 9 and and CC cord-319626-f1b3ygg0 111 10 lyophilized lyophilize VBN cord-319626-f1b3ygg0 111 11 . . . cord-319626-f1b3ygg0 112 1 The the DT cord-319626-f1b3ygg0 112 2 borate borate NN cord-319626-f1b3ygg0 112 3 was be VBD cord-319626-f1b3ygg0 112 4 removed remove VBN cord-319626-f1b3ygg0 112 5 by by IN cord-319626-f1b3ygg0 112 6 repeated repeat VBN cord-319626-f1b3ygg0 112 7 evaporation evaporation NN cord-319626-f1b3ygg0 112 8 with with IN cord-319626-f1b3ygg0 112 9 methanol methanol NN cord-319626-f1b3ygg0 112 10 at at IN cord-319626-f1b3ygg0 112 11 40 40 CD cord-319626-f1b3ygg0 112 12 ° ° NN cord-319626-f1b3ygg0 112 13 C c NN cord-319626-f1b3ygg0 112 14 . . . cord-319626-f1b3ygg0 113 1 The the DT cord-319626-f1b3ygg0 113 2 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 113 3 were be VBD cord-319626-f1b3ygg0 113 4 finally finally RB cord-319626-f1b3ygg0 113 5 resuspended resuspend VBN cord-319626-f1b3ygg0 113 6 in in IN cord-319626-f1b3ygg0 113 7 200 200 CD cord-319626-f1b3ygg0 114 1 ~t ~t JJ cord-319626-f1b3ygg0 114 2 distilled distil VBN cord-319626-f1b3ygg0 114 3 H20 h20 NN cord-319626-f1b3ygg0 114 4 . . . cord-319626-f1b3ygg0 115 1 The the DT cord-319626-f1b3ygg0 115 2 final final JJ cord-319626-f1b3ygg0 115 3 recovery recovery NN cord-319626-f1b3ygg0 115 4 of of IN cord-319626-f1b3ygg0 115 5 [ [ -LRB- cord-319626-f1b3ygg0 115 6 3H]-Iabeled 3h]-iabeled CD cord-319626-f1b3ygg0 115 7 material material NN cord-319626-f1b3ygg0 115 8 was be VBD cord-319626-f1b3ygg0 115 9 80 80 CD cord-319626-f1b3ygg0 115 10 - - SYM cord-319626-f1b3ygg0 115 11 90 90 CD cord-319626-f1b3ygg0 115 12 % % NN cord-319626-f1b3ygg0 115 13 . . . cord-319626-f1b3ygg0 116 1 One one CD cord-319626-f1b3ygg0 116 2 - - HYPH cord-319626-f1b3ygg0 116 3 half half NN cord-319626-f1b3ygg0 116 4 of of IN cord-319626-f1b3ygg0 116 5 the the DT cord-319626-f1b3ygg0 116 6 material material NN cord-319626-f1b3ygg0 116 7 was be VBD cord-319626-f1b3ygg0 116 8 applied apply VBN cord-319626-f1b3ygg0 116 9 directly directly RB cord-319626-f1b3ygg0 116 10 to to IN cord-319626-f1b3ygg0 116 11 a a DT cord-319626-f1b3ygg0 116 12 P4 p4 NN cord-319626-f1b3ygg0 116 13 ( ( -LRB- cord-319626-f1b3ygg0 116 14 200 200 CD cord-319626-f1b3ygg0 116 15 - - SYM cord-319626-f1b3ygg0 116 16 400 400 CD cord-319626-f1b3ygg0 116 17 mesh mesh NN cord-319626-f1b3ygg0 116 18 ) ) -RRB- cord-319626-f1b3ygg0 116 19 column column NN cord-319626-f1b3ygg0 116 20 ( ( -LRB- cord-319626-f1b3ygg0 116 21 90 90 CD cord-319626-f1b3ygg0 116 22 × × NNP cord-319626-f1b3ygg0 116 23 1.5 1.5 CD cord-319626-f1b3ygg0 116 24 cm cm NNS cord-319626-f1b3ygg0 116 25 ) ) -RRB- cord-319626-f1b3ygg0 116 26 , , , cord-319626-f1b3ygg0 116 27 equilibrated equilibrate VBN cord-319626-f1b3ygg0 116 28 , , , cord-319626-f1b3ygg0 116 29 and and CC cord-319626-f1b3ygg0 116 30 run run VBN cord-319626-f1b3ygg0 116 31 in in IN cord-319626-f1b3ygg0 116 32 0A 0A NNP cord-319626-f1b3ygg0 116 33 M M NNP cord-319626-f1b3ygg0 116 34 NaHCO3 NaHCO3 NNP cord-319626-f1b3ygg0 116 35 , , , cord-319626-f1b3ygg0 116 36 pH pH NNP cord-319626-f1b3ygg0 116 37 8.6 8.6 CD cord-319626-f1b3ygg0 116 38 . . . cord-319626-f1b3ygg0 117 1 The the DT cord-319626-f1b3ygg0 117 2 remainder remainder NN cord-319626-f1b3ygg0 117 3 was be VBD cord-319626-f1b3ygg0 117 4 first first RB cord-319626-f1b3ygg0 117 5 digested digest VBN cord-319626-f1b3ygg0 117 6 with with IN cord-319626-f1b3ygg0 117 7 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 117 8 from from IN cord-319626-f1b3ygg0 117 9 Vibrio Vibrio NNP cord-319626-f1b3ygg0 117 10 cholerae cholerae NN cord-319626-f1b3ygg0 117 11 at at IN cord-319626-f1b3ygg0 117 12 a a DT cord-319626-f1b3ygg0 117 13 final final JJ cord-319626-f1b3ygg0 117 14 concentration concentration NN cord-319626-f1b3ygg0 117 15 of of IN cord-319626-f1b3ygg0 117 16 I I NNP cord-319626-f1b3ygg0 117 17 U U NNP cord-319626-f1b3ygg0 117 18 / / SYM cord-319626-f1b3ygg0 117 19 ml ml XX cord-319626-f1b3ygg0 117 20 in in IN cord-319626-f1b3ygg0 117 21 50 50 CD cord-319626-f1b3ygg0 117 22 mM mm CD cord-319626-f1b3ygg0 117 23 sodium sodium NN cord-319626-f1b3ygg0 117 24 acetate acetate NN cord-319626-f1b3ygg0 117 25 buffer buffer NN cord-319626-f1b3ygg0 117 26 , , , cord-319626-f1b3ygg0 117 27 pH pH NNP cord-319626-f1b3ygg0 117 28 5.6 5.6 CD cord-319626-f1b3ygg0 117 29 , , , cord-319626-f1b3ygg0 117 30 containing contain VBG cord-319626-f1b3ygg0 117 31 154 154 CD cord-319626-f1b3ygg0 117 32 mM mm CD cord-319626-f1b3ygg0 117 33 NaCI naci NN cord-319626-f1b3ygg0 117 34 and and CC cord-319626-f1b3ygg0 117 35 9 9 CD cord-319626-f1b3ygg0 117 36 mM mm CD cord-319626-f1b3ygg0 117 37 CaC1 CaC1 NNPS cord-319626-f1b3ygg0 117 38 , , , cord-319626-f1b3ygg0 117 39 . . . cord-319626-f1b3ygg0 118 1 After after IN cord-319626-f1b3ygg0 118 2 incubation incubation NN cord-319626-f1b3ygg0 118 3 under under IN cord-319626-f1b3ygg0 118 4 a a DT cord-319626-f1b3ygg0 118 5 toluene toluene JJ cord-319626-f1b3ygg0 118 6 atmosphere atmosphere NN cord-319626-f1b3ygg0 118 7 for for IN cord-319626-f1b3ygg0 118 8 24 24 CD cord-319626-f1b3ygg0 118 9 h h NN cord-319626-f1b3ygg0 118 10 at at IN cord-319626-f1b3ygg0 118 11 37 37 CD cord-319626-f1b3ygg0 118 12 ° ° NN cord-319626-f1b3ygg0 118 13 C c NN cord-319626-f1b3ygg0 118 14 ( ( -LRB- cord-319626-f1b3ygg0 118 15 Niemann Niemann NNP cord-319626-f1b3ygg0 118 16 and and CC cord-319626-f1b3ygg0 118 17 Klenk Klenk NNP cord-319626-f1b3ygg0 118 18 , , , cord-319626-f1b3ygg0 118 19 1981 1981 CD cord-319626-f1b3ygg0 118 20 ) ) -RRB- cord-319626-f1b3ygg0 118 21 , , , cord-319626-f1b3ygg0 118 22 the the DT cord-319626-f1b3ygg0 118 23 reaction reaction NN cord-319626-f1b3ygg0 118 24 was be VBD cord-319626-f1b3ygg0 118 25 stopped stop VBN cord-319626-f1b3ygg0 118 26 by by IN cord-319626-f1b3ygg0 118 27 boiling boil VBG cord-319626-f1b3ygg0 118 28 , , , cord-319626-f1b3ygg0 118 29 and and CC cord-319626-f1b3ygg0 118 30 the the DT cord-319626-f1b3ygg0 118 31 samples sample NNS cord-319626-f1b3ygg0 118 32 were be VBD cord-319626-f1b3ygg0 118 33 lyophilized lyophilize VBN cord-319626-f1b3ygg0 118 34 and and CC cord-319626-f1b3ygg0 118 35 then then RB cord-319626-f1b3ygg0 118 36 resuspended resuspend VBD cord-319626-f1b3ygg0 118 37 in in IN cord-319626-f1b3ygg0 118 38 100 100 CD cord-319626-f1b3ygg0 118 39 I.tl i.tl CD cord-319626-f1b3ygg0 118 40 of of IN cord-319626-f1b3ygg0 118 41 distilled distilled JJ cord-319626-f1b3ygg0 118 42 H.,O h.,o NN cord-319626-f1b3ygg0 118 43 before before IN cord-319626-f1b3ygg0 118 44 application application NN cord-319626-f1b3ygg0 118 45 to to IN cord-319626-f1b3ygg0 118 46 the the DT cord-319626-f1b3ygg0 118 47 P4 P4 NNP cord-319626-f1b3ygg0 118 48 column column NN cord-319626-f1b3ygg0 118 49 . . . cord-319626-f1b3ygg0 119 1 [ [ -LRB- cord-319626-f1b3ygg0 119 2 3H]Glucose3mannose~/-acetylglucosamine 3H]Glucose3mannose~/-acetylglucosamine NNP cord-319626-f1b3ygg0 119 3 was be VBD cord-319626-f1b3ygg0 119 4 prepared prepare VBN cord-319626-f1b3ygg0 119 5 from from IN cord-319626-f1b3ygg0 119 6 Chinese chinese JJ cord-319626-f1b3ygg0 119 7 hamster hamster NN cord-319626-f1b3ygg0 119 8 ovary ovary NN cord-319626-f1b3ygg0 119 9 ( ( -LRB- cord-319626-f1b3ygg0 119 10 CHO CHO NNP cord-319626-f1b3ygg0 119 11 ) ) -RRB- cord-319626-f1b3ygg0 120 1 ( ( -LRB- cord-319626-f1b3ygg0 120 2 clone clone NNP cord-319626-f1b3ygg0 120 3 15B 15B NNP cord-319626-f1b3ygg0 120 4 cells cell NNS cord-319626-f1b3ygg0 120 5 ) ) -RRB- cord-319626-f1b3ygg0 120 6 infected infect VBN cord-319626-f1b3ygg0 120 7 with with IN cord-319626-f1b3ygg0 120 8 vesicular vesicular JJ cord-319626-f1b3ygg0 120 9 stomatitis stomatitis NN cord-319626-f1b3ygg0 120 10 virus virus NN cord-319626-f1b3ygg0 120 11 as as IN cord-319626-f1b3ygg0 120 12 described describe VBN cord-319626-f1b3ygg0 120 13 by by IN cord-319626-f1b3ygg0 120 14 Featherstone Featherstone NNP cord-319626-f1b3ygg0 120 15 et et NNP cord-319626-f1b3ygg0 120 16 al al NNP cord-319626-f1b3ygg0 120 17 . . . cord-319626-f1b3ygg0 121 1 ( ( -LRB- cord-319626-f1b3ygg0 121 2 1985 1985 CD cord-319626-f1b3ygg0 121 3 ) ) -RRB- cord-319626-f1b3ygg0 121 4 . . . cord-319626-f1b3ygg0 122 1 The the DT cord-319626-f1b3ygg0 122 2 glycopeptides glycopeptide NNS cord-319626-f1b3ygg0 122 3 were be VBD cord-319626-f1b3ygg0 122 4 prepared prepare VBN cord-319626-f1b3ygg0 122 5 and and CC cord-319626-f1b3ygg0 122 6 the the DT cord-319626-f1b3ygg0 122 7 dolicol dolicol NN cord-319626-f1b3ygg0 122 8 - - HYPH cord-319626-f1b3ygg0 122 9 linked link VBN cord-319626-f1b3ygg0 122 10 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 122 11 in in IN cord-319626-f1b3ygg0 122 12 the the DT cord-319626-f1b3ygg0 122 13 Tx Tx NNP cord-319626-f1b3ygg0 122 14 - - HYPH cord-319626-f1b3ygg0 122 15 ll4 ll4 NN cord-319626-f1b3ygg0 122 16 pellet pellet NN cord-319626-f1b3ygg0 122 17 was be VBD cord-319626-f1b3ygg0 122 18 released release VBN cord-319626-f1b3ygg0 122 19 by by IN cord-319626-f1b3ygg0 122 20 endoglycosidase endoglycosidase NNP cord-319626-f1b3ygg0 122 21 H H NNP cord-319626-f1b3ygg0 122 22 digestion digestion NN cord-319626-f1b3ygg0 122 23 . . . cord-319626-f1b3ygg0 123 1 [ [ -LRB- cord-319626-f1b3ygg0 123 2 3HlGall3(l--3)-GalNAcitol 3hlgall3(l--3)-galnacitol CD cord-319626-f1b3ygg0 123 3 was be VBD cord-319626-f1b3ygg0 123 4 prepared prepare VBN cord-319626-f1b3ygg0 123 5 by by IN cord-319626-f1b3ygg0 123 6 reduction reduction NN cord-319626-f1b3ygg0 123 7 of of IN cord-319626-f1b3ygg0 123 8 Gall3(1-'3)-GalNAc Gall3(1-'3)-GalNAc NNP cord-319626-f1b3ygg0 123 9 ( ( -LRB- cord-319626-f1b3ygg0 123 10 generously generously RB cord-319626-f1b3ygg0 123 11 supplied supply VBN cord-319626-f1b3ygg0 123 12 by by IN cord-319626-f1b3ygg0 123 13 Dr. Dr. NNP cord-319626-f1b3ygg0 123 14 W. W. NNP cord-319626-f1b3ygg0 123 15 Gielen Gielen NNP cord-319626-f1b3ygg0 123 16 , , , cord-319626-f1b3ygg0 123 17 University University NNP cord-319626-f1b3ygg0 123 18 of of IN cord-319626-f1b3ygg0 123 19 Cologne Cologne NNP cord-319626-f1b3ygg0 123 20 , , , cord-319626-f1b3ygg0 123 21 FRG FRG NNP cord-319626-f1b3ygg0 123 22 ) ) -RRB- cord-319626-f1b3ygg0 123 23 , , , cord-319626-f1b3ygg0 123 24 with with IN cord-319626-f1b3ygg0 123 25 NaB3H4 NaB3H4 NNP cord-319626-f1b3ygg0 123 26 ( ( -LRB- cord-319626-f1b3ygg0 123 27 Li Li NNP cord-319626-f1b3ygg0 123 28 et et NNP cord-319626-f1b3ygg0 123 29 al al NNP cord-319626-f1b3ygg0 123 30 . . NNP cord-319626-f1b3ygg0 123 31 , , , cord-319626-f1b3ygg0 123 32 1978 1978 CD cord-319626-f1b3ygg0 123 33 ) ) -RRB- cord-319626-f1b3ygg0 123 34 . . . cord-319626-f1b3ygg0 124 1 [ [ -LRB- cord-319626-f1b3ygg0 124 2 t4C]GalNAcitol t4C]GalNAcitol NNP cord-319626-f1b3ygg0 124 3 was be VBD cord-319626-f1b3ygg0 124 4 prepared prepare VBN cord-319626-f1b3ygg0 124 5 by by IN cord-319626-f1b3ygg0 124 6 acid acid JJ cord-319626-f1b3ygg0 124 7 hydrolysis hydrolysis NN cord-319626-f1b3ygg0 124 8 of of IN cord-319626-f1b3ygg0 124 9 UDP UDP NNP cord-319626-f1b3ygg0 124 10 - - HYPH cord-319626-f1b3ygg0 124 11 N n NN cord-319626-f1b3ygg0 124 12 - - HYPH cord-319626-f1b3ygg0 124 13 acetyl acetyl NN cord-319626-f1b3ygg0 124 14 - - HYPH cord-319626-f1b3ygg0 124 15 D-[1-t4C]galactosamine d-[1-t4c]galactosamine JJ cord-319626-f1b3ygg0 125 1 ( ( -LRB- cord-319626-f1b3ygg0 125 2 Cummings Cummings NNP cord-319626-f1b3ygg0 125 3 et et FW cord-319626-f1b3ygg0 125 4 al al NNP cord-319626-f1b3ygg0 125 5 . . NNP cord-319626-f1b3ygg0 125 6 , , , cord-319626-f1b3ygg0 125 7 1983 1983 CD cord-319626-f1b3ygg0 125 8 ) ) -RRB- cord-319626-f1b3ygg0 125 9 , , , cord-319626-f1b3ygg0 125 10 followed follow VBN cord-319626-f1b3ygg0 125 11 by by IN cord-319626-f1b3ygg0 125 12 reduction reduction NN cord-319626-f1b3ygg0 125 13 with with IN cord-319626-f1b3ygg0 125 14 NaBH4 NaBH4 NNP cord-319626-f1b3ygg0 125 15 ( ( -LRB- cord-319626-f1b3ygg0 125 16 Li Li NNP cord-319626-f1b3ygg0 125 17 et et NNP cord-319626-f1b3ygg0 125 18 al al NNP cord-319626-f1b3ygg0 125 19 . . NNP cord-319626-f1b3ygg0 125 20 , , , cord-319626-f1b3ygg0 125 21 1978 1978 CD cord-319626-f1b3ygg0 125 22 ) ) -RRB- cord-319626-f1b3ygg0 125 23 . . . cord-319626-f1b3ygg0 125 24 _SP cord-319626-f1b3ygg0 126 1 At at IN cord-319626-f1b3ygg0 126 2 37 37 CD cord-319626-f1b3ygg0 126 3 ° ° NN cord-319626-f1b3ygg0 126 4 C c NN cord-319626-f1b3ygg0 126 5 , , , cord-319626-f1b3ygg0 126 6 sac(- sac(- NNP cord-319626-f1b3ygg0 126 7 ) ) -RRB- cord-319626-f1b3ygg0 126 8 cells cell NNS cord-319626-f1b3ygg0 126 9 release release VBP cord-319626-f1b3ygg0 126 10 progeny progeny NN cord-319626-f1b3ygg0 126 11 virus virus NN cord-319626-f1b3ygg0 126 12 exponentially exponentially RB cord-319626-f1b3ygg0 126 13 , , , cord-319626-f1b3ygg0 126 14 beginning begin VBG cord-319626-f1b3ygg0 126 15 at at IN cord-319626-f1b3ygg0 126 16 ~5 ~5 NNP cord-319626-f1b3ygg0 126 17 h h XX cord-319626-f1b3ygg0 126 18 after after IN cord-319626-f1b3ygg0 126 19 the the DT cord-319626-f1b3ygg0 126 20 start start NN cord-319626-f1b3ygg0 126 21 of of IN cord-319626-f1b3ygg0 126 22 infection infection NN cord-319626-f1b3ygg0 126 23 and and CC cord-319626-f1b3ygg0 126 24 continuing continue VBG cord-319626-f1b3ygg0 126 25 until until IN cord-319626-f1b3ygg0 126 26 the the DT cord-319626-f1b3ygg0 126 27 cells cell NNS cord-319626-f1b3ygg0 126 28 lyse lyse VBP cord-319626-f1b3ygg0 126 29 . . . cord-319626-f1b3ygg0 127 1 The the DT cord-319626-f1b3ygg0 127 2 release release NN cord-319626-f1b3ygg0 127 3 of of IN cord-319626-f1b3ygg0 127 4 E1 e1 NN cord-319626-f1b3ygg0 127 5 molecules molecule NNS cord-319626-f1b3ygg0 127 6 in in IN cord-319626-f1b3ygg0 127 7 virions virion NNS cord-319626-f1b3ygg0 127 8 parallels parallel VBZ cord-319626-f1b3ygg0 127 9 the the DT cord-319626-f1b3ygg0 127 10 release release NN cord-319626-f1b3ygg0 127 11 of of IN cord-319626-f1b3ygg0 127 12 virus virus NN cord-319626-f1b3ygg0 127 13 particles particle NNS cord-319626-f1b3ygg0 127 14 consistent consistent JJ cord-319626-f1b3ygg0 127 15 with with IN cord-319626-f1b3ygg0 127 16 the the DT cord-319626-f1b3ygg0 127 17 known know VBN cord-319626-f1b3ygg0 127 18 dependence dependence NN cord-319626-f1b3ygg0 127 19 of of IN cord-319626-f1b3ygg0 127 20 budding bud VBG cord-319626-f1b3ygg0 127 21 on on IN cord-319626-f1b3ygg0 127 22 the the DT cord-319626-f1b3ygg0 127 23 E1 e1 NN cord-319626-f1b3ygg0 127 24 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 127 25 ( ( -LRB- cord-319626-f1b3ygg0 127 26 Tooze Tooze NNP cord-319626-f1b3ygg0 127 27 , , , cord-319626-f1b3ygg0 127 28 1987 1987 CD cord-319626-f1b3ygg0 127 29 ) ) -RRB- cord-319626-f1b3ygg0 127 30 . . . cord-319626-f1b3ygg0 128 1 Holmes Holmes NNP cord-319626-f1b3ygg0 128 2 and and CC cord-319626-f1b3ygg0 128 3 Behnke Behnke NNP cord-319626-f1b3ygg0 128 4 ( ( -LRB- cord-319626-f1b3ygg0 128 5 1981 1981 CD cord-319626-f1b3ygg0 128 6 ) ) -RRB- cord-319626-f1b3ygg0 128 7 observed observe VBD cord-319626-f1b3ygg0 128 8 in in IN cord-319626-f1b3ygg0 128 9 a a DT cord-319626-f1b3ygg0 128 10 preliminary preliminary JJ cord-319626-f1b3ygg0 128 11 morphological morphological JJ cord-319626-f1b3ygg0 128 12 study study NN cord-319626-f1b3ygg0 128 13 that that IN cord-319626-f1b3ygg0 128 14 at at IN cord-319626-f1b3ygg0 128 15 32 32 CD cord-319626-f1b3ygg0 128 16 ° ° NN cord-319626-f1b3ygg0 128 17 C c NN cord-319626-f1b3ygg0 128 18 virions virion NNS cord-319626-f1b3ygg0 128 19 were be VBD cord-319626-f1b3ygg0 128 20 assembled assemble VBN cord-319626-f1b3ygg0 128 21 , , , cord-319626-f1b3ygg0 128 22 but but CC cord-319626-f1b3ygg0 128 23 accumulated accumulate VBN cord-319626-f1b3ygg0 128 24 within within IN cord-319626-f1b3ygg0 128 25 the the DT cord-319626-f1b3ygg0 128 26 infected infected JJ cord-319626-f1b3ygg0 128 27 cell cell NN cord-319626-f1b3ygg0 128 28 instead instead RB cord-319626-f1b3ygg0 128 29 of of IN cord-319626-f1b3ygg0 128 30 being be VBG cord-319626-f1b3ygg0 128 31 released release VBN cord-319626-f1b3ygg0 128 32 . . . cord-319626-f1b3ygg0 129 1 To to TO cord-319626-f1b3ygg0 129 2 confirm confirm VB cord-319626-f1b3ygg0 129 3 this this DT cord-319626-f1b3ygg0 129 4 observation observation NN cord-319626-f1b3ygg0 129 5 , , , cord-319626-f1b3ygg0 129 6 infected infected JJ cord-319626-f1b3ygg0 129 7 sac(- sac(- NNS cord-319626-f1b3ygg0 129 8 ) ) -RRB- cord-319626-f1b3ygg0 129 9 cells cell NNS cord-319626-f1b3ygg0 129 10 were be VBD cord-319626-f1b3ygg0 129 11 incubated incubate VBN cord-319626-f1b3ygg0 129 12 for for IN cord-319626-f1b3ygg0 129 13 6 6 CD cord-319626-f1b3ygg0 129 14 h h NN cord-319626-f1b3ygg0 129 15 at at IN cord-319626-f1b3ygg0 129 16 37 37 CD cord-319626-f1b3ygg0 129 17 ° ° NN cord-319626-f1b3ygg0 129 18 C c NN cord-319626-f1b3ygg0 129 19 and and CC cord-319626-f1b3ygg0 129 20 the the DT cord-319626-f1b3ygg0 129 21 release release NN cord-319626-f1b3ygg0 129 22 of of IN cord-319626-f1b3ygg0 129 23 infectious infectious JJ cord-319626-f1b3ygg0 129 24 virus virus NN cord-319626-f1b3ygg0 129 25 was be VBD cord-319626-f1b3ygg0 129 26 measured measure VBN cord-319626-f1b3ygg0 129 27 by by IN cord-319626-f1b3ygg0 129 28 a a DT cord-319626-f1b3ygg0 129 29 plaque plaque NN cord-319626-f1b3ygg0 129 30 titration titration NN cord-319626-f1b3ygg0 129 31 assay assay NN cord-319626-f1b3ygg0 129 32 over over IN cord-319626-f1b3ygg0 129 33 the the DT cord-319626-f1b3ygg0 129 34 next next JJ cord-319626-f1b3ygg0 129 35 6 6 CD cord-319626-f1b3ygg0 129 36 h h NN cord-319626-f1b3ygg0 129 37 in in IN cord-319626-f1b3ygg0 129 38 cultures culture NNS cord-319626-f1b3ygg0 129 39 either either CC cord-319626-f1b3ygg0 129 40 kept keep VBD cord-319626-f1b3ygg0 129 41 at at IN cord-319626-f1b3ygg0 129 42 37 37 CD cord-319626-f1b3ygg0 129 43 ° ° NN cord-319626-f1b3ygg0 129 44 C c NN cord-319626-f1b3ygg0 129 45 or or CC cord-319626-f1b3ygg0 129 46 shifted shift VBD cord-319626-f1b3ygg0 129 47 to to IN cord-319626-f1b3ygg0 129 48 31 31 CD cord-319626-f1b3ygg0 129 49 ° ° NN cord-319626-f1b3ygg0 129 50 C c NN cord-319626-f1b3ygg0 129 51 . . . cord-319626-f1b3ygg0 130 1 As as IN cord-319626-f1b3ygg0 130 2 an an DT cord-319626-f1b3ygg0 130 3 added add VBN cord-319626-f1b3ygg0 130 4 precaution precaution NN cord-319626-f1b3ygg0 130 5 , , , cord-319626-f1b3ygg0 130 6 cells cell NNS cord-319626-f1b3ygg0 130 7 were be VBD cord-319626-f1b3ygg0 130 8 treated treat VBN cord-319626-f1b3ygg0 130 9 for for IN cord-319626-f1b3ygg0 130 10 2 2 CD cord-319626-f1b3ygg0 130 11 - - SYM cord-319626-f1b3ygg0 130 12 3 3 CD cord-319626-f1b3ygg0 130 13 min min NN cord-319626-f1b3ygg0 130 14 with with IN cord-319626-f1b3ygg0 130 15 proteinase proteinase NN cord-319626-f1b3ygg0 130 16 K K NNP cord-319626-f1b3ygg0 130 17 3.5 3.5 CD cord-319626-f1b3ygg0 130 18 h h NN cord-319626-f1b3ygg0 130 19 after after IN cord-319626-f1b3ygg0 130 20 infection infection NN cord-319626-f1b3ygg0 130 21 to to TO cord-319626-f1b3ygg0 130 22 remove remove VB cord-319626-f1b3ygg0 130 23 any any DT cord-319626-f1b3ygg0 130 24 virions virion NNS cord-319626-f1b3ygg0 130 25 from from IN cord-319626-f1b3ygg0 130 26 the the DT cord-319626-f1b3ygg0 130 27 infecting infect VBG cord-319626-f1b3ygg0 130 28 inoculum inoculum NN cord-319626-f1b3ygg0 130 29 still still RB cord-319626-f1b3ygg0 130 30 remaining remain VBG cord-319626-f1b3ygg0 130 31 on on IN cord-319626-f1b3ygg0 130 32 the the DT cord-319626-f1b3ygg0 130 33 cell cell NN cord-319626-f1b3ygg0 130 34 surface surface NN cord-319626-f1b3ygg0 130 35 . . . cord-319626-f1b3ygg0 131 1 This this DT cord-319626-f1b3ygg0 131 2 ensured ensure VBD cord-319626-f1b3ygg0 131 3 that that IN cord-319626-f1b3ygg0 131 4 only only RB cord-319626-f1b3ygg0 131 5 released release VBN cord-319626-f1b3ygg0 131 6 progeny progeny NN cord-319626-f1b3ygg0 131 7 particles particle NNS cord-319626-f1b3ygg0 131 8 were be VBD cord-319626-f1b3ygg0 131 9 measured measure VBN cord-319626-f1b3ygg0 131 10 . . . cord-319626-f1b3ygg0 132 1 Infected infected JJ cord-319626-f1b3ygg0 132 2 cells cell NNS cord-319626-f1b3ygg0 132 3 were be VBD cord-319626-f1b3ygg0 132 4 labeled label VBN cord-319626-f1b3ygg0 132 5 from from IN cord-319626-f1b3ygg0 132 6 6 6 CD cord-319626-f1b3ygg0 132 7 to to IN cord-319626-f1b3ygg0 132 8 9 9 CD cord-319626-f1b3ygg0 132 9 h h NN cord-319626-f1b3ygg0 132 10 after after IN cord-319626-f1b3ygg0 132 11 infection infection NN cord-319626-f1b3ygg0 132 12 with with IN cord-319626-f1b3ygg0 133 1 [ [ -LRB- cord-319626-f1b3ygg0 133 2 3~S]methionine 3~s]methionine CD cord-319626-f1b3ygg0 133 3 in in IN cord-319626-f1b3ygg0 133 4 MEM MEM NNP cord-319626-f1b3ygg0 133 5 - - HYPH cord-319626-f1b3ygg0 133 6 met meet VBD cord-319626-f1b3ygg0 133 7 containing contain VBG cord-319626-f1b3ygg0 133 8 5 5 CD cord-319626-f1b3ygg0 133 9 Ixg Ixg NNP cord-319626-f1b3ygg0 133 10 / / SYM cord-319626-f1b3ygg0 133 11 p p NN cord-319626-f1b3ygg0 133 12 . . . cord-319626-f1b3ygg0 134 1 I -PRON- PRP cord-319626-f1b3ygg0 134 2 cold cold VBP cord-319626-f1b3ygg0 134 3 methionine methionine NN cord-319626-f1b3ygg0 134 4 at at IN cord-319626-f1b3ygg0 134 5 37 37 CD cord-319626-f1b3ygg0 134 6 or or CC cord-319626-f1b3ygg0 134 7 31 31 CD cord-319626-f1b3ygg0 134 8 ° ° NN cord-319626-f1b3ygg0 134 9 C c NN cord-319626-f1b3ygg0 134 10 . . . cord-319626-f1b3ygg0 135 1 At at IN cord-319626-f1b3ygg0 135 2 the the DT cord-319626-f1b3ygg0 135 3 end end NN cord-319626-f1b3ygg0 135 4 of of IN cord-319626-f1b3ygg0 135 5 the the DT cord-319626-f1b3ygg0 135 6 labeling labeling NN cord-319626-f1b3ygg0 135 7 period period NN cord-319626-f1b3ygg0 135 8 the the DT cord-319626-f1b3ygg0 135 9 cells cell NNS cord-319626-f1b3ygg0 135 10 were be VBD cord-319626-f1b3ygg0 135 11 treated treat VBN cord-319626-f1b3ygg0 135 12 with with IN cord-319626-f1b3ygg0 135 13 0.5 0.5 CD cord-319626-f1b3ygg0 135 14 mg mg NNP cord-319626-f1b3ygg0 135 15 / / SYM cord-319626-f1b3ygg0 135 16 ml ml NN cord-319626-f1b3ygg0 135 17 proteinase proteinase NN cord-319626-f1b3ygg0 135 18 K k NN cord-319626-f1b3ygg0 135 19 at at IN cord-319626-f1b3ygg0 135 20 4 4 CD cord-319626-f1b3ygg0 135 21 ° ° NN cord-319626-f1b3ygg0 135 22 C c NN cord-319626-f1b3ygg0 135 23 for for IN cord-319626-f1b3ygg0 135 24 2 2 CD cord-319626-f1b3ygg0 135 25 - - SYM cord-319626-f1b3ygg0 135 26 3 3 CD cord-319626-f1b3ygg0 135 27 min min NN cord-319626-f1b3ygg0 135 28 . . . cord-319626-f1b3ygg0 136 1 The the DT cord-319626-f1b3ygg0 136 2 proteinase proteinase NN cord-319626-f1b3ygg0 136 3 K K NNP cord-319626-f1b3ygg0 136 4 was be VBD cord-319626-f1b3ygg0 136 5 neutralized neutralize VBN cord-319626-f1b3ygg0 136 6 with with IN cord-319626-f1b3ygg0 136 7 PMSF PMSF NNP cord-319626-f1b3ygg0 136 8 and and CC cord-319626-f1b3ygg0 136 9 the the DT cord-319626-f1b3ygg0 136 10 cells cell NNS cord-319626-f1b3ygg0 136 11 were be VBD cord-319626-f1b3ygg0 136 12 pelleted pellete VBN cord-319626-f1b3ygg0 136 13 at at IN cord-319626-f1b3ygg0 136 14 4 4 CD cord-319626-f1b3ygg0 136 15 ° ° NN cord-319626-f1b3ygg0 136 16 C c NN cord-319626-f1b3ygg0 136 17 . . . cord-319626-f1b3ygg0 137 1 The the DT cord-319626-f1b3ygg0 137 2 supernatant supernatant NN cord-319626-f1b3ygg0 137 3 was be VBD cord-319626-f1b3ygg0 137 4 then then RB cord-319626-f1b3ygg0 137 5 pooled pool VBN cord-319626-f1b3ygg0 137 6 with with IN cord-319626-f1b3ygg0 137 7 the the DT cord-319626-f1b3ygg0 137 8 medium medium NN cord-319626-f1b3ygg0 137 9 collected collect VBN cord-319626-f1b3ygg0 137 10 from from IN cord-319626-f1b3ygg0 137 11 the the DT cord-319626-f1b3ygg0 137 12 cells cell NNS cord-319626-f1b3ygg0 137 13 before before IN cord-319626-f1b3ygg0 137 14 the the DT cord-319626-f1b3ygg0 137 15 proteinase proteinase NN cord-319626-f1b3ygg0 137 16 K K NNP cord-319626-f1b3ygg0 137 17 treatment treatment NN cord-319626-f1b3ygg0 137 18 and and CC cord-319626-f1b3ygg0 137 19 the the DT cord-319626-f1b3ygg0 137 20 labeled label VBN cord-319626-f1b3ygg0 137 21 virions virion NNS cord-319626-f1b3ygg0 137 22 were be VBD cord-319626-f1b3ygg0 137 23 purified purify VBN cord-319626-f1b3ygg0 137 24 on on IN cord-319626-f1b3ygg0 137 25 sucrose sucrose NN cord-319626-f1b3ygg0 137 26 gradients gradient NNS cord-319626-f1b3ygg0 137 27 . . . cord-319626-f1b3ygg0 138 1 Both both DT cord-319626-f1b3ygg0 138 2 cells cell NNS cord-319626-f1b3ygg0 138 3 and and CC cord-319626-f1b3ygg0 138 4 virions virion NNS cord-319626-f1b3ygg0 138 5 were be VBD cord-319626-f1b3ygg0 138 6 subjected subject VBN cord-319626-f1b3ygg0 138 7 to to IN cord-319626-f1b3ygg0 138 8 Tx-114 tx-114 CD cord-319626-f1b3ygg0 138 9 extraction extraction NN cord-319626-f1b3ygg0 138 10 followed follow VBN cord-319626-f1b3ygg0 138 11 by by IN cord-319626-f1b3ygg0 138 12 SDS SDS NNP cord-319626-f1b3ygg0 138 13 - - HYPH cord-319626-f1b3ygg0 138 14 PAGE PAGE NNP cord-319626-f1b3ygg0 138 15 . . . cord-319626-f1b3ygg0 139 1 * * NFP cord-319626-f1b3ygg0 139 2 Counts count VBZ cord-319626-f1b3ygg0 139 3 per per IN cord-319626-f1b3ygg0 139 4 minute minute NN cord-319626-f1b3ygg0 139 5 in in IN cord-319626-f1b3ygg0 139 6 all all DT cord-319626-f1b3ygg0 139 7 forms form NNS cord-319626-f1b3ygg0 139 8 of of IN cord-319626-f1b3ygg0 139 9 El El NNP cord-319626-f1b3ygg0 139 10 detected detect VBN cord-319626-f1b3ygg0 139 11 by by IN cord-319626-f1b3ygg0 139 12 fluorography fluorography NNP cord-319626-f1b3ygg0 139 13 , , , cord-319626-f1b3ygg0 139 14 including include VBG cord-319626-f1b3ygg0 139 15 the the DT cord-319626-f1b3ygg0 139 16 lower low JJR cord-319626-f1b3ygg0 139 17 molecular molecular JJ cord-319626-f1b3ygg0 139 18 weight weight NN cord-319626-f1b3ygg0 139 19 forms form NNS cord-319626-f1b3ygg0 139 20 of of IN cord-319626-f1b3ygg0 139 21 El El NNP cord-319626-f1b3ygg0 139 22 generated generate VBN cord-319626-f1b3ygg0 139 23 by by IN cord-319626-f1b3ygg0 139 24 proteinase proteinase NN cord-319626-f1b3ygg0 139 25 K k NN cord-319626-f1b3ygg0 139 26 digestion digestion NN cord-319626-f1b3ygg0 140 1 ( ( -LRB- cord-319626-f1b3ygg0 140 2 Rottier Rottier NNP cord-319626-f1b3ygg0 140 3 et et NNP cord-319626-f1b3ygg0 140 4 al al NNP cord-319626-f1b3ygg0 140 5 . . NNP cord-319626-f1b3ygg0 140 6 , , , cord-319626-f1b3ygg0 140 7 1984 1984 CD cord-319626-f1b3ygg0 141 1 ( ( -LRB- cord-319626-f1b3ygg0 141 2 Rottier Rottier NNP cord-319626-f1b3ygg0 141 3 et et NNP cord-319626-f1b3ygg0 141 4 al al NNP cord-319626-f1b3ygg0 141 5 . . NNP cord-319626-f1b3ygg0 141 6 , , , cord-319626-f1b3ygg0 141 7 ) ) -RRB- cord-319626-f1b3ygg0 141 8 . . . cord-319626-f1b3ygg0 142 1 1981a 1981a LS cord-319626-f1b3ygg0 142 2 Rottier Rottier NNP cord-319626-f1b3ygg0 142 3 et et NNP cord-319626-f1b3ygg0 142 4 al al NNP cord-319626-f1b3ygg0 142 5 . . NNP cord-319626-f1b3ygg0 142 6 , , , cord-319626-f1b3ygg0 142 7 1981 1981 CD cord-319626-f1b3ygg0 142 8 ) ) -RRB- cord-319626-f1b3ygg0 142 9 . . . cord-319626-f1b3ygg0 143 1 To to TO cord-319626-f1b3ygg0 143 2 eliminate eliminate VB cord-319626-f1b3ygg0 143 3 the the DT cord-319626-f1b3ygg0 143 4 possibility possibility NN cord-319626-f1b3ygg0 143 5 that that IN cord-319626-f1b3ygg0 143 6 at at IN cord-319626-f1b3ygg0 143 7 31 31 CD cord-319626-f1b3ygg0 143 8 ° ° NN cord-319626-f1b3ygg0 143 9 C C NNP cord-319626-f1b3ygg0 143 10 the the DT cord-319626-f1b3ygg0 143 11 cells cell NNS cord-319626-f1b3ygg0 143 12 were be VBD cord-319626-f1b3ygg0 143 13 releasing release VBG cord-319626-f1b3ygg0 143 14 large large JJ cord-319626-f1b3ygg0 143 15 numbers number NNS cord-319626-f1b3ygg0 143 16 of of IN cord-319626-f1b3ygg0 143 17 noninfectious noninfectious JJ cord-319626-f1b3ygg0 143 18 particles particle NNS cord-319626-f1b3ygg0 143 19 , , , cord-319626-f1b3ygg0 143 20 which which WDT cord-319626-f1b3ygg0 143 21 would would MD cord-319626-f1b3ygg0 143 22 not not RB cord-319626-f1b3ygg0 143 23 be be VB cord-319626-f1b3ygg0 143 24 scored score VBN cord-319626-f1b3ygg0 143 25 in in IN cord-319626-f1b3ygg0 143 26 an an DT cord-319626-f1b3ygg0 143 27 infectivity infectivity NN cord-319626-f1b3ygg0 143 28 assay assay NN cord-319626-f1b3ygg0 143 29 , , , cord-319626-f1b3ygg0 143 30 El El NNP cord-319626-f1b3ygg0 143 31 contained contain VBD cord-319626-f1b3ygg0 143 32 in in IN cord-319626-f1b3ygg0 143 33 virus virus NN cord-319626-f1b3ygg0 143 34 particles particle NNS cord-319626-f1b3ygg0 143 35 released release VBN cord-319626-f1b3ygg0 143 36 from from IN cord-319626-f1b3ygg0 143 37 the the DT cord-319626-f1b3ygg0 143 38 cells cell NNS cord-319626-f1b3ygg0 143 39 was be VBD cord-319626-f1b3ygg0 143 40 quantitated quantitate VBN cord-319626-f1b3ygg0 143 41 . . . cord-319626-f1b3ygg0 144 1 Infected infected JJ cord-319626-f1b3ygg0 144 2 cells cell NNS cord-319626-f1b3ygg0 144 3 were be VBD cord-319626-f1b3ygg0 144 4 labeled label VBN cord-319626-f1b3ygg0 144 5 from from IN cord-319626-f1b3ygg0 144 6 6 6 CD cord-319626-f1b3ygg0 144 7 to to IN cord-319626-f1b3ygg0 144 8 9 9 CD cord-319626-f1b3ygg0 144 9 h h NN cord-319626-f1b3ygg0 144 10 after after IN cord-319626-f1b3ygg0 144 11 infection infection NN cord-319626-f1b3ygg0 144 12 with with IN cord-319626-f1b3ygg0 144 13 pSS]methionine pss]methionine NN cord-319626-f1b3ygg0 144 14 at at IN cord-319626-f1b3ygg0 144 15 37 37 CD cord-319626-f1b3ygg0 144 16 and and CC cord-319626-f1b3ygg0 144 17 31 31 CD cord-319626-f1b3ygg0 144 18 ° ° NN cord-319626-f1b3ygg0 144 19 C c NN cord-319626-f1b3ygg0 144 20 , , , cord-319626-f1b3ygg0 144 21 the the DT cord-319626-f1b3ygg0 144 22 medium medium NN cord-319626-f1b3ygg0 144 23 was be VBD cord-319626-f1b3ygg0 144 24 collected collect VBN cord-319626-f1b3ygg0 144 25 , , , cord-319626-f1b3ygg0 144 26 and and CC cord-319626-f1b3ygg0 144 27 any any DT cord-319626-f1b3ygg0 144 28 labeled label VBN cord-319626-f1b3ygg0 144 29 virions virion NNS cord-319626-f1b3ygg0 144 30 associated associate VBN cord-319626-f1b3ygg0 144 31 with with IN cord-319626-f1b3ygg0 144 32 the the DT cord-319626-f1b3ygg0 144 33 plasma plasma NN cord-319626-f1b3ygg0 144 34 membrane membrane NN cord-319626-f1b3ygg0 144 35 were be VBD cord-319626-f1b3ygg0 144 36 removed remove VBN cord-319626-f1b3ygg0 144 37 by by IN cord-319626-f1b3ygg0 144 38 proteinase proteinase NN cord-319626-f1b3ygg0 144 39 K k NN cord-319626-f1b3ygg0 144 40 treatment treatment NN cord-319626-f1b3ygg0 144 41 at at IN cord-319626-f1b3ygg0 144 42 4 4 CD cord-319626-f1b3ygg0 144 43 ° ° NN cord-319626-f1b3ygg0 144 44 C c NN cord-319626-f1b3ygg0 144 45 and and CC cord-319626-f1b3ygg0 144 46 pooled pool VBN cord-319626-f1b3ygg0 144 47 with with IN cord-319626-f1b3ygg0 144 48 the the DT cord-319626-f1b3ygg0 144 49 virions virion NNS cord-319626-f1b3ygg0 144 50 in in IN cord-319626-f1b3ygg0 144 51 the the DT cord-319626-f1b3ygg0 144 52 medium medium NN cord-319626-f1b3ygg0 144 53 . . . cord-319626-f1b3ygg0 145 1 After after IN cord-319626-f1b3ygg0 145 2 SDS SDS NNP cord-319626-f1b3ygg0 145 3 - - HYPH cord-319626-f1b3ygg0 145 4 PAGE PAGE NNP cord-319626-f1b3ygg0 145 5 and and CC cord-319626-f1b3ygg0 145 6 fluorography fluorography NN cord-319626-f1b3ygg0 145 7 of of IN cord-319626-f1b3ygg0 145 8 virions virion NNS cord-319626-f1b3ygg0 145 9 purified purify VBN cord-319626-f1b3ygg0 145 10 from from IN cord-319626-f1b3ygg0 145 11 the the DT cord-319626-f1b3ygg0 145 12 medium medium NN cord-319626-f1b3ygg0 145 13 , , , cord-319626-f1b3ygg0 145 14 the the DT cord-319626-f1b3ygg0 145 15 E1 e1 NN cord-319626-f1b3ygg0 145 16 band band NN cord-319626-f1b3ygg0 145 17 was be VBD cord-319626-f1b3ygg0 145 18 excised excise VBN cord-319626-f1b3ygg0 145 19 and and CC cord-319626-f1b3ygg0 145 20 counted count VBN cord-319626-f1b3ygg0 145 21 . . . cord-319626-f1b3ygg0 146 1 As as IN cord-319626-f1b3ygg0 146 2 shown show VBN cord-319626-f1b3ygg0 146 3 in in IN cord-319626-f1b3ygg0 146 4 Table Table NNP cord-319626-f1b3ygg0 146 5 II II NNP cord-319626-f1b3ygg0 146 6 , , , cord-319626-f1b3ygg0 146 7 the the DT cord-319626-f1b3ygg0 146 8 6 6 CD cord-319626-f1b3ygg0 146 9 ° ° NN cord-319626-f1b3ygg0 146 10 C C NNP cord-319626-f1b3ygg0 146 11 drop drop NN cord-319626-f1b3ygg0 146 12 in in IN cord-319626-f1b3ygg0 146 13 temperature temperature NN cord-319626-f1b3ygg0 146 14 during during IN cord-319626-f1b3ygg0 146 15 the the DT cord-319626-f1b3ygg0 146 16 labeling labeling NN cord-319626-f1b3ygg0 146 17 period period NN cord-319626-f1b3ygg0 146 18 inhibited inhibit VBD cord-319626-f1b3ygg0 146 19 the the DT cord-319626-f1b3ygg0 146 20 release release NN cord-319626-f1b3ygg0 146 21 of of IN cord-319626-f1b3ygg0 146 22 virion virion NN cord-319626-f1b3ygg0 146 23 - - HYPH cord-319626-f1b3ygg0 146 24 associated associate VBN cord-319626-f1b3ygg0 146 25 E1 e1 NN cord-319626-f1b3ygg0 146 26 by by IN cord-319626-f1b3ygg0 146 27 > > XX cord-319626-f1b3ygg0 146 28 80 80 CD cord-319626-f1b3ygg0 146 29 % % NN cord-319626-f1b3ygg0 146 30 . . . cord-319626-f1b3ygg0 147 1 In in IN cord-319626-f1b3ygg0 147 2 marked marked JJ cord-319626-f1b3ygg0 147 3 contrast contrast NN cord-319626-f1b3ygg0 147 4 , , , cord-319626-f1b3ygg0 147 5 however however RB cord-319626-f1b3ygg0 147 6 , , , cord-319626-f1b3ygg0 147 7 the the DT cord-319626-f1b3ygg0 147 8 total total JJ cord-319626-f1b3ygg0 147 9 amount amount NN cord-319626-f1b3ygg0 147 10 of of IN cord-319626-f1b3ygg0 147 11 E1 e1 NN cord-319626-f1b3ygg0 147 12 synthesized synthesize VBN cord-319626-f1b3ygg0 147 13 was be VBD cord-319626-f1b3ygg0 147 14 reduced reduce VBN cord-319626-f1b3ygg0 147 15 by by IN cord-319626-f1b3ygg0 147 16 only only RB cord-319626-f1b3ygg0 147 17 , , , cord-319626-f1b3ygg0 147 18 x,20 x,20 NN cord-319626-f1b3ygg0 147 19 % % NN cord-319626-f1b3ygg0 147 20 upon upon IN cord-319626-f1b3ygg0 147 21 shifting shift VBG cord-319626-f1b3ygg0 147 22 the the DT cord-319626-f1b3ygg0 147 23 temperature temperature NN cord-319626-f1b3ygg0 147 24 to to IN cord-319626-f1b3ygg0 147 25 31 31 CD cord-319626-f1b3ygg0 147 26 ° ° NN cord-319626-f1b3ygg0 147 27 C c NN cord-319626-f1b3ygg0 147 28 ( ( -LRB- cord-319626-f1b3ygg0 147 29 Table Table NNP cord-319626-f1b3ygg0 147 30 II II NNP cord-319626-f1b3ygg0 147 31 ) ) -RRB- cord-319626-f1b3ygg0 147 32 . . . cord-319626-f1b3ygg0 148 1 Total total JJ cord-319626-f1b3ygg0 148 2 protein protein NN cord-319626-f1b3ygg0 148 3 synthesis synthesis NN cord-319626-f1b3ygg0 148 4 , , , cord-319626-f1b3ygg0 148 5 in in IN cord-319626-f1b3ygg0 148 6 both both CC cord-319626-f1b3ygg0 148 7 infected infected JJ cord-319626-f1b3ygg0 148 8 and and CC cord-319626-f1b3ygg0 148 9 uninfected uninfected JJ cord-319626-f1b3ygg0 148 10 cells cell NNS cord-319626-f1b3ygg0 148 11 , , , cord-319626-f1b3ygg0 148 12 was be VBD cord-319626-f1b3ygg0 148 13 on on IN cord-319626-f1b3ygg0 148 14 average average JJ cord-319626-f1b3ygg0 148 15 decreased decrease VBD cord-319626-f1b3ygg0 148 16 by by IN cord-319626-f1b3ygg0 148 17 only only RB cord-319626-f1b3ygg0 148 18 23 23 CD cord-319626-f1b3ygg0 148 19 % % NN cord-319626-f1b3ygg0 148 20 over over IN cord-319626-f1b3ygg0 148 21 the the DT cord-319626-f1b3ygg0 148 22 same same JJ cord-319626-f1b3ygg0 148 23 time time NN cord-319626-f1b3ygg0 148 24 period period NN cord-319626-f1b3ygg0 148 25 . . . cord-319626-f1b3ygg0 149 1 The the DT cord-319626-f1b3ygg0 149 2 discrepancy discrepancy NN cord-319626-f1b3ygg0 149 3 between between IN cord-319626-f1b3ygg0 149 4 the the DT cord-319626-f1b3ygg0 149 5 observed observed JJ cord-319626-f1b3ygg0 149 6 1,000-fold 1,000-fold CD cord-319626-f1b3ygg0 149 7 decrease decrease NN cord-319626-f1b3ygg0 149 8 in in IN cord-319626-f1b3ygg0 149 9 the the DT cord-319626-f1b3ygg0 149 10 release release NN cord-319626-f1b3ygg0 149 11 of of IN cord-319626-f1b3ygg0 149 12 infectious infectious JJ cord-319626-f1b3ygg0 149 13 virions virion NNS cord-319626-f1b3ygg0 149 14 ( ( -LRB- cord-319626-f1b3ygg0 149 15 Table table NN cord-319626-f1b3ygg0 149 16 I -PRON- PRP cord-319626-f1b3ygg0 149 17 ) ) -RRB- cord-319626-f1b3ygg0 149 18 and and CC cord-319626-f1b3ygg0 149 19 the the DT cord-319626-f1b3ygg0 149 20 fivefold fivefold JJ cord-319626-f1b3ygg0 149 21 decrease decrease NN cord-319626-f1b3ygg0 149 22 in in IN cord-319626-f1b3ygg0 149 23 the the DT cord-319626-f1b3ygg0 149 24 release release NN cord-319626-f1b3ygg0 149 25 of of IN cord-319626-f1b3ygg0 149 26 virion virion NN cord-319626-f1b3ygg0 149 27 - - HYPH cord-319626-f1b3ygg0 149 28 associated associate VBN cord-319626-f1b3ygg0 149 29 E1 e1 NN cord-319626-f1b3ygg0 149 30 ( ( -LRB- cord-319626-f1b3ygg0 149 31 Table Table NNP cord-319626-f1b3ygg0 149 32 II ii CD cord-319626-f1b3ygg0 149 33 ) ) -RRB- cord-319626-f1b3ygg0 149 34 may may MD cord-319626-f1b3ygg0 149 35 mean mean VB cord-319626-f1b3ygg0 149 36 that that IN cord-319626-f1b3ygg0 149 37 at at IN cord-319626-f1b3ygg0 149 38 31 31 CD cord-319626-f1b3ygg0 149 39 ° ° NN cord-319626-f1b3ygg0 149 40 C c NN cord-319626-f1b3ygg0 149 41 more more JJR cord-319626-f1b3ygg0 149 42 noninfectious noninfectious JJ cord-319626-f1b3ygg0 149 43 particles particle NNS cord-319626-f1b3ygg0 149 44 are be VBP cord-319626-f1b3ygg0 149 45 assembled assemble VBN cord-319626-f1b3ygg0 149 46 and and CC cord-319626-f1b3ygg0 149 47 released release VBN cord-319626-f1b3ygg0 149 48 . . . cord-319626-f1b3ygg0 150 1 However however RB cord-319626-f1b3ygg0 150 2 , , , cord-319626-f1b3ygg0 150 3 we -PRON- PRP cord-319626-f1b3ygg0 150 4 favor favor VBP cord-319626-f1b3ygg0 150 5 an an DT cord-319626-f1b3ygg0 150 6 alternative alternative JJ cord-319626-f1b3ygg0 150 7 explanation explanation NN cord-319626-f1b3ygg0 150 8 , , , cord-319626-f1b3ygg0 150 9 namely namely RB cord-319626-f1b3ygg0 150 10 , , , cord-319626-f1b3ygg0 150 11 that that DT cord-319626-f1b3ygg0 150 12 proteinase proteinase NN cord-319626-f1b3ygg0 150 13 K k NN cord-319626-f1b3ygg0 150 14 treatment treatment NN cord-319626-f1b3ygg0 150 15 damages damage NNS cord-319626-f1b3ygg0 150 16 some some DT cord-319626-f1b3ygg0 150 17 cells cell NNS cord-319626-f1b3ygg0 150 18 and and CC cord-319626-f1b3ygg0 150 19 leads lead VBZ cord-319626-f1b3ygg0 150 20 to to IN cord-319626-f1b3ygg0 150 21 artifactual artifactual JJ cord-319626-f1b3ygg0 150 22 release release NN cord-319626-f1b3ygg0 150 23 of of IN cord-319626-f1b3ygg0 150 24 intracellular intracellular JJ cord-319626-f1b3ygg0 150 25 virions virion NNS cord-319626-f1b3ygg0 150 26 because because IN cord-319626-f1b3ygg0 150 27 , , , cord-319626-f1b3ygg0 150 28 when when WRB cord-319626-f1b3ygg0 150 29 medium medium NN cord-319626-f1b3ygg0 150 30 is be VBZ cord-319626-f1b3ygg0 150 31 collected collect VBN cord-319626-f1b3ygg0 150 32 from from IN cord-319626-f1b3ygg0 150 33 cells cell NNS cord-319626-f1b3ygg0 150 34 at at IN cord-319626-f1b3ygg0 150 35 31 31 CD cord-319626-f1b3ygg0 150 36 ° ° NN cord-319626-f1b3ygg0 150 37 C c NN cord-319626-f1b3ygg0 150 38 without without IN cord-319626-f1b3ygg0 150 39 proteinase proteinase NN cord-319626-f1b3ygg0 150 40 K K NNP cord-319626-f1b3ygg0 150 41 treatment treatment NN cord-319626-f1b3ygg0 150 42 , , , cord-319626-f1b3ygg0 150 43 the the DT cord-319626-f1b3ygg0 150 44 amount amount NN cord-319626-f1b3ygg0 150 45 of of IN cord-319626-f1b3ygg0 150 46 methioninelabeled methioninelabeled JJ cord-319626-f1b3ygg0 150 47 virion virion NN cord-319626-f1b3ygg0 150 48 - - HYPH cord-319626-f1b3ygg0 150 49 associated associate VBN cord-319626-f1b3ygg0 150 50 E1 E1 NNP cord-319626-f1b3ygg0 150 51 is be VBZ cord-319626-f1b3ygg0 150 52 below below IN cord-319626-f1b3ygg0 150 53 the the DT cord-319626-f1b3ygg0 150 54 limits limit NNS cord-319626-f1b3ygg0 150 55 of of IN cord-319626-f1b3ygg0 150 56 detection detection NN cord-319626-f1b3ygg0 150 57 by by IN cord-319626-f1b3ygg0 150 58 fluorography fluorography NN cord-319626-f1b3ygg0 150 59 after after IN cord-319626-f1b3ygg0 150 60 SDS SDS NNP cord-319626-f1b3ygg0 150 61 - - HYPH cord-319626-f1b3ygg0 150 62 PAGE PAGE NNP cord-319626-f1b3ygg0 150 63 ( ( -LRB- cord-319626-f1b3ygg0 150 64 data datum NNS cord-319626-f1b3ygg0 150 65 not not RB cord-319626-f1b3ygg0 150 66 shown show VBN cord-319626-f1b3ygg0 150 67 ) ) -RRB- cord-319626-f1b3ygg0 150 68 . . . cord-319626-f1b3ygg0 151 1 Furthermore furthermore RB cord-319626-f1b3ygg0 151 2 , , , cord-319626-f1b3ygg0 151 3 electron electron NN cord-319626-f1b3ygg0 151 4 microscopic microscopic JJ cord-319626-f1b3ygg0 151 5 studies study NNS cord-319626-f1b3ygg0 151 6 of of IN cord-319626-f1b3ygg0 151 7 thin thin JJ cord-319626-f1b3ygg0 151 8 sections section NNS cord-319626-f1b3ygg0 151 9 of of IN cord-319626-f1b3ygg0 151 10 infected infected JJ cord-319626-f1b3ygg0 151 11 cells cell NNS cord-319626-f1b3ygg0 151 12 incubated incubate VBN cord-319626-f1b3ygg0 151 13 at at IN cord-319626-f1b3ygg0 151 14 31 31 CD cord-319626-f1b3ygg0 151 15 ° ° NN cord-319626-f1b3ygg0 151 16 C c NN cord-319626-f1b3ygg0 151 17 from from IN cord-319626-f1b3ygg0 151 18 7 7 CD cord-319626-f1b3ygg0 151 19 to to IN cord-319626-f1b3ygg0 151 20 24 24 CD cord-319626-f1b3ygg0 151 21 h h NN cord-319626-f1b3ygg0 151 22 after after IN cord-319626-f1b3ygg0 151 23 infection infection NN cord-319626-f1b3ygg0 151 24 reveal reveal VBP cord-319626-f1b3ygg0 151 25 very very RB cord-319626-f1b3ygg0 151 26 few few JJ cord-319626-f1b3ygg0 151 27 extracellular extracellular JJ cord-319626-f1b3ygg0 151 28 virions virion NNS cord-319626-f1b3ygg0 151 29 associated associate VBN cord-319626-f1b3ygg0 151 30 with with IN cord-319626-f1b3ygg0 151 31 the the DT cord-319626-f1b3ygg0 151 32 plasma plasma NN cord-319626-f1b3ygg0 151 33 membrane membrane NN cord-319626-f1b3ygg0 151 34 ( ( -LRB- cord-319626-f1b3ygg0 151 35 see see VB cord-319626-f1b3ygg0 151 36 below below RB cord-319626-f1b3ygg0 151 37 and and CC cord-319626-f1b3ygg0 151 38 Fig Fig NNP cord-319626-f1b3ygg0 151 39 . . . cord-319626-f1b3ygg0 151 40 10 10 CD cord-319626-f1b3ygg0 151 41 ) ) -RRB- cord-319626-f1b3ygg0 151 42 . . . cord-319626-f1b3ygg0 152 1 These these DT cord-319626-f1b3ygg0 152 2 data datum NNS cord-319626-f1b3ygg0 152 3 show show VBP cord-319626-f1b3ygg0 152 4 that that IN cord-319626-f1b3ygg0 152 5 E1 E1 NNP cord-319626-f1b3ygg0 152 6 synthesis synthesis NN cord-319626-f1b3ygg0 152 7 continues continue VBZ cord-319626-f1b3ygg0 152 8 almost almost RB cord-319626-f1b3ygg0 152 9 normally normally RB cord-319626-f1b3ygg0 152 10 at at IN cord-319626-f1b3ygg0 152 11 31 31 CD cord-319626-f1b3ygg0 152 12 ° ° NN cord-319626-f1b3ygg0 152 13 C c NN cord-319626-f1b3ygg0 152 14 but but CC cord-319626-f1b3ygg0 152 15 the the DT cord-319626-f1b3ygg0 152 16 release release NN cord-319626-f1b3ygg0 152 17 into into IN cord-319626-f1b3ygg0 152 18 the the DT cord-319626-f1b3ygg0 152 19 medium medium NN cord-319626-f1b3ygg0 152 20 of of IN cord-319626-f1b3ygg0 152 21 virions virion NNS cord-319626-f1b3ygg0 152 22 is be VBZ cord-319626-f1b3ygg0 152 23 dramatically dramatically RB cord-319626-f1b3ygg0 152 24 inhibited inhibit VBN cord-319626-f1b3ygg0 152 25 . . . cord-319626-f1b3ygg0 153 1 To to TO cord-319626-f1b3ygg0 153 2 determine determine VB cord-319626-f1b3ygg0 153 3 the the DT cord-319626-f1b3ygg0 153 4 site site NN cord-319626-f1b3ygg0 153 5 of of IN cord-319626-f1b3ygg0 153 6 accumulation accumulation NN cord-319626-f1b3ygg0 153 7 of of IN cord-319626-f1b3ygg0 153 8 virions virion NNS cord-319626-f1b3ygg0 153 9 and and CC cord-319626-f1b3ygg0 153 10 E1 e1 NN cord-319626-f1b3ygg0 153 11 at at IN cord-319626-f1b3ygg0 153 12 31 31 CD cord-319626-f1b3ygg0 153 13 ° ° NN cord-319626-f1b3ygg0 153 14 C C NNP cord-319626-f1b3ygg0 153 15 a a DT cord-319626-f1b3ygg0 153 16 combination combination NN cord-319626-f1b3ygg0 153 17 of of IN cord-319626-f1b3ygg0 153 18 both both DT cord-319626-f1b3ygg0 153 19 biochemical biochemical JJ cord-319626-f1b3ygg0 153 20 and and CC cord-319626-f1b3ygg0 153 21 morphological morphological JJ cord-319626-f1b3ygg0 153 22 techniques technique NNS cord-319626-f1b3ygg0 153 23 was be VBD cord-319626-f1b3ygg0 153 24 used use VBN cord-319626-f1b3ygg0 153 25 . . . cord-319626-f1b3ygg0 154 1 At at IN cord-319626-f1b3ygg0 154 2 37 37 CD cord-319626-f1b3ygg0 154 3 ° ° NN cord-319626-f1b3ygg0 154 4 C c NN cord-319626-f1b3ygg0 154 5 and and CC cord-319626-f1b3ygg0 154 6 8 8 CD cord-319626-f1b3ygg0 154 7 h h NN cord-319626-f1b3ygg0 154 8 after after IN cord-319626-f1b3ygg0 154 9 infection infection NN cord-319626-f1b3ygg0 154 10 , , , cord-319626-f1b3ygg0 154 11 sac(- sac(- NNP cord-319626-f1b3ygg0 154 12 ) ) -RRB- cord-319626-f1b3ygg0 154 13 cells cell NNS cord-319626-f1b3ygg0 154 14 were be VBD cord-319626-f1b3ygg0 154 15 pulsed pulse VBN cord-319626-f1b3ygg0 154 16 for for IN cord-319626-f1b3ygg0 154 17 5 5 CD cord-319626-f1b3ygg0 154 18 min min NN cord-319626-f1b3ygg0 154 19 with with IN cord-319626-f1b3ygg0 154 20 [ [ -LRB- cord-319626-f1b3ygg0 154 21 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 154 22 and and CC cord-319626-f1b3ygg0 154 23 then then RB cord-319626-f1b3ygg0 154 24 chased chase VBN cord-319626-f1b3ygg0 154 25 in in IN cord-319626-f1b3ygg0 154 26 the the DT cord-319626-f1b3ygg0 154 27 presence presence NN cord-319626-f1b3ygg0 154 28 of of IN cord-319626-f1b3ygg0 154 29 excess excess JJ cord-319626-f1b3ygg0 154 30 unlabeled unlabeled JJ cord-319626-f1b3ygg0 154 31 methionine methionine NN cord-319626-f1b3ygg0 154 32 for for IN cord-319626-f1b3ygg0 154 33 up up IN cord-319626-f1b3ygg0 154 34 to to TO cord-319626-f1b3ygg0 154 35 40 40 CD cord-319626-f1b3ygg0 154 36 min min NN cord-319626-f1b3ygg0 154 37 . . . cord-319626-f1b3ygg0 155 1 Fig Fig NNP cord-319626-f1b3ygg0 155 2 . . NNP cord-319626-f1b3ygg0 155 3 1 1 CD cord-319626-f1b3ygg0 155 4 shows show VBZ cord-319626-f1b3ygg0 155 5 that that IN cord-319626-f1b3ygg0 155 6 a a DT cord-319626-f1b3ygg0 155 7 proportion proportion NN cord-319626-f1b3ygg0 155 8 of of IN cord-319626-f1b3ygg0 155 9 the the DT cord-319626-f1b3ygg0 155 10 unglycosylated unglycosylated JJ cord-319626-f1b3ygg0 155 11 E1 E1 NNP cord-319626-f1b3ygg0 155 12 protein protein NN cord-319626-f1b3ygg0 155 13 ( ( -LRB- cord-319626-f1b3ygg0 155 14 E e NN cord-319626-f1b3ygg0 155 15 / / SYM cord-319626-f1b3ygg0 155 16 u u NNP cord-319626-f1b3ygg0 155 17 , , , cord-319626-f1b3ygg0 155 18 lane lane NN cord-319626-f1b3ygg0 155 19 1 1 CD cord-319626-f1b3ygg0 155 20 ) ) -RRB- cord-319626-f1b3ygg0 155 21 is be VBZ cord-319626-f1b3ygg0 155 22 converted convert VBN cord-319626-f1b3ygg0 155 23 after after IN cord-319626-f1b3ygg0 155 24 10 10 CD cord-319626-f1b3ygg0 155 25 min min NN cord-319626-f1b3ygg0 155 26 of of IN cord-319626-f1b3ygg0 155 27 chase chase NN cord-319626-f1b3ygg0 155 28 to to IN cord-319626-f1b3ygg0 155 29 an an DT cord-319626-f1b3ygg0 155 30 intermediate intermediate JJ cord-319626-f1b3ygg0 155 31 form form NN cord-319626-f1b3ygg0 155 32 of of IN cord-319626-f1b3ygg0 155 33 higher high JJR cord-319626-f1b3ygg0 155 34 molecular molecular JJ cord-319626-f1b3ygg0 155 35 weight weight NN cord-319626-f1b3ygg0 155 36 ( ( -LRB- cord-319626-f1b3ygg0 155 37 E e NN cord-319626-f1b3ygg0 155 38 / / SYM cord-319626-f1b3ygg0 155 39 i i PRP cord-319626-f1b3ygg0 155 40 , , , cord-319626-f1b3ygg0 155 41 lane lane NN cord-319626-f1b3ygg0 155 42 2 2 CD cord-319626-f1b3ygg0 155 43 ) ) -RRB- cord-319626-f1b3ygg0 155 44 and and CC cord-319626-f1b3ygg0 155 45 after after IN cord-319626-f1b3ygg0 155 46 " " '' cord-319626-f1b3ygg0 155 47 , , , cord-319626-f1b3ygg0 155 48 , , , cord-319626-f1b3ygg0 155 49 20 20 CD cord-319626-f1b3ygg0 155 50 min min NN cord-319626-f1b3ygg0 155 51 of of IN cord-319626-f1b3ygg0 155 52 chase chase NNP cord-319626-f1b3ygg0 155 53 ( ( -LRB- cord-319626-f1b3ygg0 155 54 lane lane NNP cord-319626-f1b3ygg0 155 55 3 3 CD cord-319626-f1b3ygg0 155 56 ) ) -RRB- cord-319626-f1b3ygg0 155 57 to to IN cord-319626-f1b3ygg0 155 58 the the DT cord-319626-f1b3ygg0 155 59 mature mature JJ cord-319626-f1b3ygg0 155 60 forms form NNS cord-319626-f1b3ygg0 155 61 ( ( -LRB- cord-319626-f1b3ygg0 155 62 E e NN cord-319626-f1b3ygg0 155 63 / / SYM cord-319626-f1b3ygg0 155 64 m m NNP cord-319626-f1b3ygg0 155 65 ) ) -RRB- cord-319626-f1b3ygg0 155 66 , , , cord-319626-f1b3ygg0 155 67 which which WDT cord-319626-f1b3ygg0 155 68 in in IN cord-319626-f1b3ygg0 155 69 this this DT cord-319626-f1b3ygg0 155 70 experiment experiment NN cord-319626-f1b3ygg0 155 71 consisted consist VBD cord-319626-f1b3ygg0 155 72 of of IN cord-319626-f1b3ygg0 155 73 three three CD cord-319626-f1b3ygg0 155 74 distinct distinct JJ cord-319626-f1b3ygg0 155 75 polypeptides polypeptide NNS cord-319626-f1b3ygg0 155 76 differing differ VBG cord-319626-f1b3ygg0 155 77 in in IN cord-319626-f1b3ygg0 155 78 molecular molecular JJ cord-319626-f1b3ygg0 155 79 mass mass NN cord-319626-f1b3ygg0 155 80 by by IN cord-319626-f1b3ygg0 155 81 ~4 ~4 . cord-319626-f1b3ygg0 156 1 kD. kD. NNP cord-319626-f1b3ygg0 156 2 Three three CD cord-319626-f1b3ygg0 156 3 forms form NNS cord-319626-f1b3ygg0 156 4 of of IN cord-319626-f1b3ygg0 156 5 E1 e1 NN cord-319626-f1b3ygg0 156 6 were be VBD cord-319626-f1b3ygg0 156 7 always always RB cord-319626-f1b3ygg0 156 8 detected detect VBN cord-319626-f1b3ygg0 156 9 ( ( -LRB- cord-319626-f1b3ygg0 156 10 Fig Fig NNP cord-319626-f1b3ygg0 156 11 . . NNP cord-319626-f1b3ygg0 156 12 1 1 CD cord-319626-f1b3ygg0 156 13 , , , cord-319626-f1b3ygg0 156 14 lane lane NN cord-319626-f1b3ygg0 156 15 4 4 CD cord-319626-f1b3ygg0 156 16 ) ) -RRB- cord-319626-f1b3ygg0 156 17 , , , cord-319626-f1b3ygg0 156 18 and and CC cord-319626-f1b3ygg0 156 19 in in IN cord-319626-f1b3ygg0 156 20 some some DT cord-319626-f1b3ygg0 156 21 experiments experiment NNS cord-319626-f1b3ygg0 156 22 varying vary VBG cord-319626-f1b3ygg0 156 23 but but CC cord-319626-f1b3ygg0 156 24 small small JJ cord-319626-f1b3ygg0 156 25 amounts amount NNS cord-319626-f1b3ygg0 156 26 of of IN cord-319626-f1b3ygg0 156 27 two two CD cord-319626-f1b3ygg0 156 28 additional additional JJ cord-319626-f1b3ygg0 156 29 higher high JJR cord-319626-f1b3ygg0 156 30 molecular molecular JJ cord-319626-f1b3ygg0 156 31 mass mass NN cord-319626-f1b3ygg0 156 32 forms form NNS cord-319626-f1b3ygg0 156 33 were be VBD cord-319626-f1b3ygg0 156 34 resolved resolve VBN cord-319626-f1b3ygg0 156 35 . . . cord-319626-f1b3ygg0 157 1 The the DT cord-319626-f1b3ygg0 157 2 observed observed JJ cord-319626-f1b3ygg0 157 3 increases increase NNS cord-319626-f1b3ygg0 157 4 in in IN cord-319626-f1b3ygg0 157 5 molecular molecular JJ cord-319626-f1b3ygg0 157 6 mass mass NN cord-319626-f1b3ygg0 157 7 must must MD cord-319626-f1b3ygg0 157 8 be be VB cord-319626-f1b3ygg0 157 9 due due JJ cord-319626-f1b3ygg0 157 10 to to IN cord-319626-f1b3ygg0 157 11 the the DT cord-319626-f1b3ygg0 157 12 addition addition NN cord-319626-f1b3ygg0 157 13 of of IN cord-319626-f1b3ygg0 157 14 O o NN cord-319626-f1b3ygg0 157 15 - - HYPH cord-319626-f1b3ygg0 157 16 linked link VBN cord-319626-f1b3ygg0 157 17 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 157 18 ( ( -LRB- cord-319626-f1b3ygg0 157 19 Holmes Holmes NNP cord-319626-f1b3ygg0 157 20 et et NNP cord-319626-f1b3ygg0 157 21 al al NNP cord-319626-f1b3ygg0 157 22 . . NNP cord-319626-f1b3ygg0 157 23 , , , cord-319626-f1b3ygg0 157 24 1981a 1981a CD cord-319626-f1b3ygg0 157 25 ; ; : cord-319626-f1b3ygg0 157 26 Niemann Niemann NNP cord-319626-f1b3ygg0 157 27 and and CC cord-319626-f1b3ygg0 157 28 Klenk Klenk NNP cord-319626-f1b3ygg0 157 29 , , , cord-319626-f1b3ygg0 157 30 1981 1981 CD cord-319626-f1b3ygg0 157 31 ; ; : cord-319626-f1b3ygg0 157 32 Rottier Rottier NNP cord-319626-f1b3ygg0 157 33 et et NNP cord-319626-f1b3ygg0 157 34 al al NNP cord-319626-f1b3ygg0 157 35 . . NNP cord-319626-f1b3ygg0 157 36 , , , cord-319626-f1b3ygg0 157 37 1981 1981 CD cord-319626-f1b3ygg0 157 38 ) ) -RRB- cord-319626-f1b3ygg0 157 39 , , , cord-319626-f1b3ygg0 157 40 which which WDT cord-319626-f1b3ygg0 157 41 is be VBZ cord-319626-f1b3ygg0 157 42 the the DT cord-319626-f1b3ygg0 157 43 only only RB cord-319626-f1b3ygg0 157 44 known know VBN cord-319626-f1b3ygg0 157 45 posttranslational posttranslational JJ cord-319626-f1b3ygg0 157 46 modification modification NN cord-319626-f1b3ygg0 157 47 of of IN cord-319626-f1b3ygg0 157 48 El El NNP cord-319626-f1b3ygg0 157 49 . . . cord-319626-f1b3ygg0 158 1 The the DT cord-319626-f1b3ygg0 158 2 intermediate intermediate JJ cord-319626-f1b3ygg0 158 3 form form NN cord-319626-f1b3ygg0 158 4 EI~ EI~ NNP cord-319626-f1b3ygg0 158 5 has have VBZ cord-319626-f1b3ygg0 158 6 not not RB cord-319626-f1b3ygg0 158 7 , , , cord-319626-f1b3ygg0 158 8 however however RB cord-319626-f1b3ygg0 158 9 , , , cord-319626-f1b3ygg0 158 10 been be VBN cord-319626-f1b3ygg0 158 11 previously previously RB cord-319626-f1b3ygg0 158 12 detected detect VBN cord-319626-f1b3ygg0 158 13 and and CC cord-319626-f1b3ygg0 158 14 the the DT cord-319626-f1b3ygg0 158 15 heterogeneity heterogeneity NN cord-319626-f1b3ygg0 158 16 of of IN cord-319626-f1b3ygg0 158 17 mature mature JJ cord-319626-f1b3ygg0 158 18 forms form NNS cord-319626-f1b3ygg0 158 19 resolved resolve VBN cord-319626-f1b3ygg0 158 20 in in IN cord-319626-f1b3ygg0 158 21 Fig Fig NNP cord-319626-f1b3ygg0 158 22 . . . cord-319626-f1b3ygg0 159 1 1 1 CD cord-319626-f1b3ygg0 159 2 was be VBD cord-319626-f1b3ygg0 159 3 not not RB cord-319626-f1b3ygg0 159 4 seen see VBN cord-319626-f1b3ygg0 159 5 in in IN cord-319626-f1b3ygg0 159 6 previous previous JJ cord-319626-f1b3ygg0 159 7 work work NN cord-319626-f1b3ygg0 159 8 with with IN cord-319626-f1b3ygg0 159 9 the the DT cord-319626-f1b3ygg0 159 10 same same JJ cord-319626-f1b3ygg0 159 11 virus virus NN cord-319626-f1b3ygg0 159 12 propagated propagate VBN cord-319626-f1b3ygg0 159 13 in in IN cord-319626-f1b3ygg0 159 14 a a DT cord-319626-f1b3ygg0 159 15 different different JJ cord-319626-f1b3ygg0 159 16 cell cell NN cord-319626-f1b3ygg0 159 17 line line NN cord-319626-f1b3ygg0 159 18 ( ( -LRB- cord-319626-f1b3ygg0 159 19 e.g. e.g. RB cord-319626-f1b3ygg0 159 20 , , , cord-319626-f1b3ygg0 159 21 Niemann Niemann NNP cord-319626-f1b3ygg0 159 22 et et NNP cord-319626-f1b3ygg0 159 23 al al NNP cord-319626-f1b3ygg0 159 24 . . NNP cord-319626-f1b3ygg0 159 25 , , , cord-319626-f1b3ygg0 159 26 1982 1982 CD cord-319626-f1b3ygg0 159 27 ) ) -RRB- cord-319626-f1b3ygg0 159 28 . . . cord-319626-f1b3ygg0 160 1 To to TO cord-319626-f1b3ygg0 160 2 determine determine VB cord-319626-f1b3ygg0 160 3 the the DT cord-319626-f1b3ygg0 160 4 sugar sugar NN cord-319626-f1b3ygg0 160 5 composition composition NN cord-319626-f1b3ygg0 160 6 of of IN cord-319626-f1b3ygg0 160 7 the the DT cord-319626-f1b3ygg0 160 8 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 160 9 bound bind VBN cord-319626-f1b3ygg0 160 10 to to IN cord-319626-f1b3ygg0 160 11 each each DT cord-319626-f1b3ygg0 160 12 of of IN cord-319626-f1b3ygg0 160 13 the the DT cord-319626-f1b3ygg0 160 14 E1 e1 NN cord-319626-f1b3ygg0 160 15 forms form NNS cord-319626-f1b3ygg0 160 16 , , , cord-319626-f1b3ygg0 160 17 infected infected JJ cord-319626-f1b3ygg0 160 18 cells cell NNS cord-319626-f1b3ygg0 160 19 were be VBD cord-319626-f1b3ygg0 160 20 labeled label VBN cord-319626-f1b3ygg0 160 21 from from IN cord-319626-f1b3ygg0 160 22 7 7 CD cord-319626-f1b3ygg0 160 23 to to IN cord-319626-f1b3ygg0 160 24 8.5 8.5 CD cord-319626-f1b3ygg0 160 25 h h NN cord-319626-f1b3ygg0 160 26 after after IN cord-319626-f1b3ygg0 160 27 infection infection NN cord-319626-f1b3ygg0 160 28 with with IN cord-319626-f1b3ygg0 160 29 3H 3h CD cord-319626-f1b3ygg0 160 30 - - HYPH cord-319626-f1b3ygg0 160 31 precursors precursor NNS cord-319626-f1b3ygg0 160 32 of of IN cord-319626-f1b3ygg0 160 33 each each DT cord-319626-f1b3ygg0 160 34 of of IN cord-319626-f1b3ygg0 160 35 the the DT cord-319626-f1b3ygg0 160 36 sugars sugar NNS cord-319626-f1b3ygg0 160 37 expected expect VBN cord-319626-f1b3ygg0 160 38 to to TO cord-319626-f1b3ygg0 160 39 be be VB cord-319626-f1b3ygg0 160 40 present present JJ cord-319626-f1b3ygg0 160 41 in in IN cord-319626-f1b3ygg0 160 42 the the DT cord-319626-f1b3ygg0 160 43 O o NN cord-319626-f1b3ygg0 160 44 - - HYPH cord-319626-f1b3ygg0 160 45 linked link VBN cord-319626-f1b3ygg0 160 46 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 160 47 , , , cord-319626-f1b3ygg0 160 48 extracted extract VBN cord-319626-f1b3ygg0 160 49 with with IN cord-319626-f1b3ygg0 160 50 Tx Tx NNP cord-319626-f1b3ygg0 160 51 - - HYPH cord-319626-f1b3ygg0 160 52 ll4 ll4 NNP cord-319626-f1b3ygg0 160 53 , , , cord-319626-f1b3ygg0 160 54 and and CC cord-319626-f1b3ygg0 160 55 subjected subject VBN cord-319626-f1b3ygg0 160 56 to to IN cord-319626-f1b3ygg0 160 57 SDS SDS NNP cord-319626-f1b3ygg0 160 58 - - HYPH cord-319626-f1b3ygg0 160 59 PAGE PAGE NNP cord-319626-f1b3ygg0 160 60 followed follow VBN cord-319626-f1b3ygg0 160 61 by by IN cord-319626-f1b3ygg0 160 62 fluorography fluorography NNP cord-319626-f1b3ygg0 160 63 . . . cord-319626-f1b3ygg0 161 1 As as IN cord-319626-f1b3ygg0 161 2 expected expect VBN cord-319626-f1b3ygg0 161 3 , , , cord-319626-f1b3ygg0 161 4 Elu Elu NNP cord-319626-f1b3ygg0 161 5 , , , cord-319626-f1b3ygg0 161 6 the the DT cord-319626-f1b3ygg0 161 7 unglycosylated unglycosylated JJ cord-319626-f1b3ygg0 161 8 form form NN cord-319626-f1b3ygg0 161 9 of of IN cord-319626-f1b3ygg0 161 10 El El NNP cord-319626-f1b3ygg0 161 11 , , , cord-319626-f1b3ygg0 161 12 was be VBD cord-319626-f1b3ygg0 161 13 not not RB cord-319626-f1b3ygg0 161 14 labeled label VBN cord-319626-f1b3ygg0 161 15 by by IN cord-319626-f1b3ygg0 161 16 any any DT cord-319626-f1b3ygg0 161 17 of of IN cord-319626-f1b3ygg0 161 18 the the DT cord-319626-f1b3ygg0 161 19 3H 3h CD cord-319626-f1b3ygg0 161 20 - - HYPH cord-319626-f1b3ygg0 161 21 sugars sugar NNS cord-319626-f1b3ygg0 161 22 ( ( -LRB- cord-319626-f1b3ygg0 161 23 Fig Fig NNP cord-319626-f1b3ygg0 161 24 . . NNP cord-319626-f1b3ygg0 161 25 2 2 CD cord-319626-f1b3ygg0 161 26 , , , cord-319626-f1b3ygg0 161 27 cf cf NNP cord-319626-f1b3ygg0 161 28 . . NNP cord-319626-f1b3ygg0 161 29 lane lane NNP cord-319626-f1b3ygg0 161 30 1 1 CD cord-319626-f1b3ygg0 161 31 with with IN cord-319626-f1b3ygg0 161 32 lanes lane NNS cord-319626-f1b3ygg0 161 33 2 2 CD cord-319626-f1b3ygg0 161 34 - - SYM cord-319626-f1b3ygg0 161 35 4 4 CD cord-319626-f1b3ygg0 161 36 ) ) -RRB- cord-319626-f1b3ygg0 161 37 . . . cord-319626-f1b3ygg0 162 1 [ [ -LRB- cord-319626-f1b3ygg0 162 2 3H 3h NN cord-319626-f1b3ygg0 162 3 ] ] -RRB- cord-319626-f1b3ygg0 162 4 Galactose galactose NN cord-319626-f1b3ygg0 162 5 and and CC cord-319626-f1b3ygg0 162 6 [ [ -LRB- cord-319626-f1b3ygg0 162 7 3H]N 3h]n NN cord-319626-f1b3ygg0 162 8 - - HYPH cord-319626-f1b3ygg0 162 9 acetyl acetyl NN cord-319626-f1b3ygg0 162 10 - - HYPH cord-319626-f1b3ygg0 162 11 mannosamine mannosamine NNP cord-319626-f1b3ygg0 162 12 are be VBP cord-319626-f1b3ygg0 162 13 specific specific JJ cord-319626-f1b3ygg0 162 14 metabolic metabolic JJ cord-319626-f1b3ygg0 162 15 precursors precursor NNS cord-319626-f1b3ygg0 162 16 of of IN cord-319626-f1b3ygg0 162 17 Gal Gal NNP cord-319626-f1b3ygg0 162 18 and and CC cord-319626-f1b3ygg0 162 19 NANA NANA NNP cord-319626-f1b3ygg0 162 20 , , , cord-319626-f1b3ygg0 162 21 respectively respectively RB cord-319626-f1b3ygg0 163 1 ( ( -LRB- cord-319626-f1b3ygg0 163 2 Neutra Neutra NNP cord-319626-f1b3ygg0 163 3 and and CC cord-319626-f1b3ygg0 163 4 Leblond Leblond NNP cord-319626-f1b3ygg0 163 5 , , , cord-319626-f1b3ygg0 163 6 1966 1966 CD cord-319626-f1b3ygg0 163 7 ; ; : cord-319626-f1b3ygg0 163 8 Monaco Monaco NNP cord-319626-f1b3ygg0 163 9 and and CC cord-319626-f1b3ygg0 163 10 Robbins Robbins NNP cord-319626-f1b3ygg0 163 11 , , , cord-319626-f1b3ygg0 163 12 1973 1973 CD cord-319626-f1b3ygg0 163 13 ) ) -RRB- cord-319626-f1b3ygg0 163 14 , , , cord-319626-f1b3ygg0 163 15 and and CC cord-319626-f1b3ygg0 163 16 these these DT cord-319626-f1b3ygg0 163 17 were be VBD cord-319626-f1b3ygg0 163 18 found find VBN cord-319626-f1b3ygg0 163 19 to to TO cord-319626-f1b3ygg0 163 20 label label VB cord-319626-f1b3ygg0 163 21 only only RB cord-319626-f1b3ygg0 163 22 the the DT cord-319626-f1b3ygg0 163 23 Elm Elm NNP cord-319626-f1b3ygg0 163 24 forms form NNS cord-319626-f1b3ygg0 163 25 ( ( -LRB- cord-319626-f1b3ygg0 163 26 Fig Fig NNP cord-319626-f1b3ygg0 163 27 . . NNP cord-319626-f1b3ygg0 163 28 2 2 CD cord-319626-f1b3ygg0 163 29 , , , cord-319626-f1b3ygg0 163 30 cf cf NNP cord-319626-f1b3ygg0 163 31 . . NNP cord-319626-f1b3ygg0 163 32 lane lane NNP cord-319626-f1b3ygg0 163 33 1 1 CD cord-319626-f1b3ygg0 163 34 with with IN cord-319626-f1b3ygg0 163 35 lanes lane NNS cord-319626-f1b3ygg0 163 36 3 3 CD cord-319626-f1b3ygg0 163 37 and and CC cord-319626-f1b3ygg0 163 38 4 4 CD cord-319626-f1b3ygg0 163 39 ) ) -RRB- cord-319626-f1b3ygg0 163 40 . . . cord-319626-f1b3ygg0 164 1 The the DT cord-319626-f1b3ygg0 164 2 presence presence NN cord-319626-f1b3ygg0 164 3 of of IN cord-319626-f1b3ygg0 164 4 NANA NANA NNP cord-319626-f1b3ygg0 164 5 in in IN cord-319626-f1b3ygg0 164 6 Elm Elm NNP cord-319626-f1b3ygg0 164 7 was be VBD cord-319626-f1b3ygg0 164 8 also also RB cord-319626-f1b3ygg0 164 9 confirmed confirm VBN cord-319626-f1b3ygg0 164 10 by by IN cord-319626-f1b3ygg0 164 11 treatment treatment NN cord-319626-f1b3ygg0 164 12 of of IN cord-319626-f1b3ygg0 164 13 [ [ -LRB- cord-319626-f1b3ygg0 164 14 35S]methionine 35s]methionine CD cord-319626-f1b3ygg0 164 15 - - HYPH cord-319626-f1b3ygg0 164 16 labeled label VBN cord-319626-f1b3ygg0 164 17 E1 e1 NN cord-319626-f1b3ygg0 164 18 with with IN cord-319626-f1b3ygg0 164 19 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 164 20 . . . cord-319626-f1b3ygg0 165 1 The the DT cord-319626-f1b3ygg0 165 2 Elm Elm NNP cord-319626-f1b3ygg0 165 3 polypeptides polypeptide NNS cord-319626-f1b3ygg0 165 4 alone alone RB cord-319626-f1b3ygg0 165 5 were be VBD cord-319626-f1b3ygg0 165 6 reduced reduce VBN cord-319626-f1b3ygg0 165 7 in in IN cord-319626-f1b3ygg0 165 8 molecular molecular JJ cord-319626-f1b3ygg0 165 9 mass mass NN cord-319626-f1b3ygg0 165 10 to to IN cord-319626-f1b3ygg0 165 11 a a DT cord-319626-f1b3ygg0 165 12 form form NN cord-319626-f1b3ygg0 165 13 which which WDT cord-319626-f1b3ygg0 165 14 migrated migrate VBD cord-319626-f1b3ygg0 165 15 slightly slightly RB cord-319626-f1b3ygg0 165 16 slower slow JJR cord-319626-f1b3ygg0 165 17 than than IN cord-319626-f1b3ygg0 166 1 EI~ EI~ NNP cord-319626-f1b3ygg0 166 2 ( ( -LRB- cord-319626-f1b3ygg0 166 3 Fig Fig NNP cord-319626-f1b3ygg0 166 4 . . . cord-319626-f1b3ygg0 166 5 3 3 CD cord-319626-f1b3ygg0 166 6 , , , cord-319626-f1b3ygg0 166 7 lane lane NN cord-319626-f1b3ygg0 166 8 4 4 CD cord-319626-f1b3ygg0 166 9 ) ) -RRB- cord-319626-f1b3ygg0 166 10 and and CC cord-319626-f1b3ygg0 166 11 was be VBD cord-319626-f1b3ygg0 166 12 assumed assume VBN cord-319626-f1b3ygg0 166 13 to to TO cord-319626-f1b3ygg0 166 14 have have VB cord-319626-f1b3ygg0 166 15 O o NN cord-319626-f1b3ygg0 166 16 - - HYPH cord-319626-f1b3ygg0 166 17 linked link VBN cord-319626-f1b3ygg0 166 18 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 166 19 composed compose VBN cord-319626-f1b3ygg0 166 20 of of IN cord-319626-f1b3ygg0 166 21 Gal Gal NNP cord-319626-f1b3ygg0 166 22 - - HYPH cord-319626-f1b3ygg0 166 23 GalNAc GalNAc NNP cord-319626-f1b3ygg0 166 24 . . . cord-319626-f1b3ygg0 167 1 Mature mature JJ cord-319626-f1b3ygg0 167 2 forms form NNS cord-319626-f1b3ygg0 167 3 of of IN cord-319626-f1b3ygg0 167 4 E1 e1 NN cord-319626-f1b3ygg0 167 5 are be VBP cord-319626-f1b3ygg0 167 6 known know VBN cord-319626-f1b3ygg0 167 7 to to TO cord-319626-f1b3ygg0 167 8 contain contain VB cord-319626-f1b3ygg0 167 9 two two CD cord-319626-f1b3ygg0 167 10 types type NNS cord-319626-f1b3ygg0 167 11 of of IN cord-319626-f1b3ygg0 167 12 O o NN cord-319626-f1b3ygg0 167 13 - - HYPH cord-319626-f1b3ygg0 167 14 linked link VBN cord-319626-f1b3ygg0 167 15 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 167 16 : : : cord-319626-f1b3ygg0 167 17 ( ( -LRB- cord-319626-f1b3ygg0 167 18 a a LS cord-319626-f1b3ygg0 167 19 ) ) -RRB- cord-319626-f1b3ygg0 167 20 a a DT cord-319626-f1b3ygg0 167 21 disialylated disialylate VBN cord-319626-f1b3ygg0 167 22 form form NN cord-319626-f1b3ygg0 167 23 ( ( -LRB- cord-319626-f1b3ygg0 167 24 Niemann Niemann NNP cord-319626-f1b3ygg0 167 25 et et NNP cord-319626-f1b3ygg0 167 26 al al NNP cord-319626-f1b3ygg0 167 27 . . NNP cord-319626-f1b3ygg0 167 28 , , , cord-319626-f1b3ygg0 167 29 1984 1984 CD cord-319626-f1b3ygg0 167 30 ) ) -RRB- cord-319626-f1b3ygg0 167 31 , , , cord-319626-f1b3ygg0 167 32 The the DT cord-319626-f1b3ygg0 167 33 intermediate intermediate JJ cord-319626-f1b3ygg0 167 34 form form NN cord-319626-f1b3ygg0 167 35 of of IN cord-319626-f1b3ygg0 167 36 E1 e1 NN cord-319626-f1b3ygg0 167 37 ( ( -LRB- cord-319626-f1b3ygg0 167 38 Eli Eli NNP cord-319626-f1b3ygg0 167 39 ) ) -RRB- cord-319626-f1b3ygg0 167 40 was be VBD cord-319626-f1b3ygg0 167 41 labeled label VBN cord-319626-f1b3ygg0 167 42 by by IN cord-319626-f1b3ygg0 167 43 [ [ -LRB- cord-319626-f1b3ygg0 167 44 3H]glucosamine 3h]glucosamine NN cord-319626-f1b3ygg0 167 45 ( ( -LRB- cord-319626-f1b3ygg0 167 46 Fig Fig NNP cord-319626-f1b3ygg0 167 47 . . NNP cord-319626-f1b3ygg0 167 48 2 2 CD cord-319626-f1b3ygg0 167 49 , , , cord-319626-f1b3ygg0 167 50 lane lane NN cord-319626-f1b3ygg0 167 51 2 2 CD cord-319626-f1b3ygg0 167 52 ) ) -RRB- cord-319626-f1b3ygg0 168 1 ( ( -LRB- cord-319626-f1b3ygg0 168 2 Fig Fig NNP cord-319626-f1b3ygg0 168 3 . . NNP cord-319626-f1b3ygg0 168 4 2 2 CD cord-319626-f1b3ygg0 168 5 , , , cord-319626-f1b3ygg0 168 6 lane lane NN cord-319626-f1b3ygg0 168 7 4 4 CD cord-319626-f1b3ygg0 168 8 ) ) -RRB- cord-319626-f1b3ygg0 168 9 . . . cord-319626-f1b3ygg0 169 1 EI~ EI~ NNP cord-319626-f1b3ygg0 169 2 was be VBD cord-319626-f1b3ygg0 169 3 also also RB cord-319626-f1b3ygg0 169 4 not not RB cord-319626-f1b3ygg0 169 5 labeled label VBN cord-319626-f1b3ygg0 169 6 by by IN cord-319626-f1b3ygg0 169 7 [ [ -LRB- cord-319626-f1b3ygg0 169 8 3H]Gal 3h]gal CD cord-319626-f1b3ygg0 169 9 ( ( -LRB- cord-319626-f1b3ygg0 169 10 Fig Fig NNP cord-319626-f1b3ygg0 169 11 . . NNP cord-319626-f1b3ygg0 169 12 2 2 CD cord-319626-f1b3ygg0 169 13 , , , cord-319626-f1b3ygg0 169 14 lane lane NN cord-319626-f1b3ygg0 169 15 3 3 CD cord-319626-f1b3ygg0 169 16 ) ) -RRB- cord-319626-f1b3ygg0 169 17 . . . cord-319626-f1b3ygg0 170 1 Based base VBN cord-319626-f1b3ygg0 170 2 on on IN cord-319626-f1b3ygg0 170 3 the the DT cord-319626-f1b3ygg0 170 4 known know VBN cord-319626-f1b3ygg0 170 5 structure structure NN cord-319626-f1b3ygg0 170 6 of of IN cord-319626-f1b3ygg0 170 7 the the DT cord-319626-f1b3ygg0 170 8 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 170 9 , , , cord-319626-f1b3ygg0 170 10 these these DT cord-319626-f1b3ygg0 170 11 data datum NNS cord-319626-f1b3ygg0 170 12 indicate indicate VBP cord-319626-f1b3ygg0 170 13 that that IN cord-319626-f1b3ygg0 170 14 GalNAc GalNAc NNP cord-319626-f1b3ygg0 170 15 is be VBZ cord-319626-f1b3ygg0 170 16 most most RBS cord-319626-f1b3ygg0 170 17 likely likely RB cord-319626-f1b3ygg0 170 18 the the DT cord-319626-f1b3ygg0 170 19 only only JJ cord-319626-f1b3ygg0 170 20 sugar sugar NN cord-319626-f1b3ygg0 170 21 present present JJ cord-319626-f1b3ygg0 170 22 in in IN cord-319626-f1b3ygg0 170 23 the the DT cord-319626-f1b3ygg0 170 24 EI~ EI~ NNP cord-319626-f1b3ygg0 170 25 form form NN cord-319626-f1b3ygg0 170 26 . . . cord-319626-f1b3ygg0 171 1 The the DT cord-319626-f1b3ygg0 171 2 Journal Journal NNP cord-319626-f1b3ygg0 171 3 of of IN cord-319626-f1b3ygg0 171 4 Cell Cell NNP cord-319626-f1b3ygg0 171 5 Biology Biology NNP cord-319626-f1b3ygg0 171 6 , , , cord-319626-f1b3ygg0 171 7 Volume volume NN cord-319626-f1b3ygg0 171 8 106 106 CD cord-319626-f1b3ygg0 171 9 , , , cord-319626-f1b3ygg0 171 10 1988 1988 CD cord-319626-f1b3ygg0 171 11 were be VBD cord-319626-f1b3ygg0 171 12 pulse pulse NN cord-319626-f1b3ygg0 171 13 labeled label VBN cord-319626-f1b3ygg0 171 14 for for IN cord-319626-f1b3ygg0 171 15 10 10 CD cord-319626-f1b3ygg0 171 16 min min NN cord-319626-f1b3ygg0 171 17 with with IN cord-319626-f1b3ygg0 171 18 0.2 0.2 CD cord-319626-f1b3ygg0 171 19 IsCi/~l IsCi/~l NNP cord-319626-f1b3ygg0 171 20 [ [ -LRB- cord-319626-f1b3ygg0 171 21 3sS]methionine 3ss]methionine CD cord-319626-f1b3ygg0 171 22 or or CC cord-319626-f1b3ygg0 171 23 pulse pulse NN cord-319626-f1b3ygg0 171 24 labeled label VBN cord-319626-f1b3ygg0 171 25 and and CC cord-319626-f1b3ygg0 171 26 chased chase VBN cord-319626-f1b3ygg0 171 27 for for IN cord-319626-f1b3ygg0 171 28 60 60 CD cord-319626-f1b3ygg0 171 29 min min NN cord-319626-f1b3ygg0 171 30 at at IN cord-319626-f1b3ygg0 171 31 37 37 CD cord-319626-f1b3ygg0 171 32 ° ° NN cord-319626-f1b3ygg0 171 33 C c NN cord-319626-f1b3ygg0 171 34 . . . cord-319626-f1b3ygg0 172 1 After after IN cord-319626-f1b3ygg0 172 2 immunoprecipitation immunoprecipitation NN cord-319626-f1b3ygg0 172 3 , , , cord-319626-f1b3ygg0 172 4 the the DT cord-319626-f1b3ygg0 172 5 samples sample NNS cord-319626-f1b3ygg0 172 6 were be VBD cord-319626-f1b3ygg0 172 7 divided divide VBN cord-319626-f1b3ygg0 172 8 in in IN cord-319626-f1b3ygg0 172 9 half half NN cord-319626-f1b3ygg0 172 10 and and CC cord-319626-f1b3ygg0 172 11 were be VBD cord-319626-f1b3ygg0 172 12 either either CC cord-319626-f1b3ygg0 172 13 ( ( -LRB- cord-319626-f1b3ygg0 172 14 lanes lane NNS cord-319626-f1b3ygg0 172 15 1 1 CD cord-319626-f1b3ygg0 172 16 and and CC cord-319626-f1b3ygg0 172 17 2 2 CD cord-319626-f1b3ygg0 172 18 ) ) -RRB- cord-319626-f1b3ygg0 172 19 incubated incubate VBN cord-319626-f1b3ygg0 172 20 without without IN cord-319626-f1b3ygg0 172 21 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 172 22 in in IN cord-319626-f1b3ygg0 172 23 the the DT cord-319626-f1b3ygg0 172 24 digestion digestion NN cord-319626-f1b3ygg0 172 25 buffer buffer NN cord-319626-f1b3ygg0 172 26 or or CC cord-319626-f1b3ygg0 172 27 ( ( -LRB- cord-319626-f1b3ygg0 172 28 lanes lane NNS cord-319626-f1b3ygg0 172 29 3 3 CD cord-319626-f1b3ygg0 172 30 and and CC cord-319626-f1b3ygg0 172 31 4 4 CD cord-319626-f1b3ygg0 172 32 ) ) -RRB- cord-319626-f1b3ygg0 172 33 with with IN cord-319626-f1b3ygg0 172 34 0.2 0.2 CD cord-319626-f1b3ygg0 172 35 mU mU NNS cord-319626-f1b3ygg0 172 36 / / SYM cord-319626-f1b3ygg0 172 37 ml ml NN cord-319626-f1b3ygg0 172 38 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 172 39 in in IN cord-319626-f1b3ygg0 172 40 the the DT cord-319626-f1b3ygg0 172 41 digestion digestion NN cord-319626-f1b3ygg0 172 42 buffer buffer NN cord-319626-f1b3ygg0 172 43 at at IN cord-319626-f1b3ygg0 172 44 37 37 CD cord-319626-f1b3ygg0 172 45 ° ° NN cord-319626-f1b3ygg0 172 46 C c NN cord-319626-f1b3ygg0 172 47 overnight overnight RB cord-319626-f1b3ygg0 172 48 . . . cord-319626-f1b3ygg0 173 1 Equal equal JJ cord-319626-f1b3ygg0 173 2 volumes volume NNS cord-319626-f1b3ygg0 173 3 were be VBD cord-319626-f1b3ygg0 173 4 loaded load VBN cord-319626-f1b3ygg0 173 5 onto onto IN cord-319626-f1b3ygg0 173 6 each each DT cord-319626-f1b3ygg0 173 7 lane lane NN cord-319626-f1b3ygg0 173 8 . . . cord-319626-f1b3ygg0 174 1 * * NFP cord-319626-f1b3ygg0 174 2 , , , cord-319626-f1b3ygg0 174 3 the the DT cord-319626-f1b3ygg0 174 4 major major JJ cord-319626-f1b3ygg0 174 5 digestion digestion NN cord-319626-f1b3ygg0 174 6 product product NN cord-319626-f1b3ygg0 174 7 after after IN cord-319626-f1b3ygg0 174 8 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 174 9 treatment treatment NN cord-319626-f1b3ygg0 174 10 . . . cord-319626-f1b3ygg0 175 1 To to TO cord-319626-f1b3ygg0 175 2 confirm confirm VB cord-319626-f1b3ygg0 175 3 that that IN cord-319626-f1b3ygg0 175 4 the the DT cord-319626-f1b3ygg0 175 5 intermediate intermediate JJ cord-319626-f1b3ygg0 175 6 form form NN cord-319626-f1b3ygg0 175 7 of of IN cord-319626-f1b3ygg0 175 8 E1 e1 NN cord-319626-f1b3ygg0 175 9 results result NNS cord-319626-f1b3ygg0 175 10 from from IN cord-319626-f1b3ygg0 175 11 the the DT cord-319626-f1b3ygg0 175 12 addition addition NN cord-319626-f1b3ygg0 175 13 of of IN cord-319626-f1b3ygg0 175 14 GalNAc GalNAc NNP cord-319626-f1b3ygg0 175 15 , , , cord-319626-f1b3ygg0 175 16 infected infected JJ cord-319626-f1b3ygg0 175 17 cells cell NNS cord-319626-f1b3ygg0 175 18 were be VBD cord-319626-f1b3ygg0 175 19 labeled label VBN cord-319626-f1b3ygg0 175 20 from from IN cord-319626-f1b3ygg0 175 21 4 4 CD cord-319626-f1b3ygg0 175 22 to to IN cord-319626-f1b3ygg0 175 23 16 16 CD cord-319626-f1b3ygg0 175 24 h h NN cord-319626-f1b3ygg0 175 25 after after IN cord-319626-f1b3ygg0 175 26 infection infection NN cord-319626-f1b3ygg0 175 27 with with IN cord-319626-f1b3ygg0 175 28 [ [ -LRB- cord-319626-f1b3ygg0 175 29 3H]glucosamine 3h]glucosamine CD cord-319626-f1b3ygg0 175 30 at at IN cord-319626-f1b3ygg0 175 31 37 37 CD cord-319626-f1b3ygg0 175 32 ° ° NN cord-319626-f1b3ygg0 175 33 C c NN cord-319626-f1b3ygg0 175 34 . . . cord-319626-f1b3ygg0 176 1 The the DT cord-319626-f1b3ygg0 176 2 virus virus NN cord-319626-f1b3ygg0 176 3 was be VBD cord-319626-f1b3ygg0 176 4 purified purify VBN cord-319626-f1b3ygg0 176 5 from from IN cord-319626-f1b3ygg0 176 6 the the DT cord-319626-f1b3ygg0 176 7 medium medium NN cord-319626-f1b3ygg0 176 8 , , , cord-319626-f1b3ygg0 176 9 solubilized solubilize VBN cord-319626-f1b3ygg0 176 10 in in IN cord-319626-f1b3ygg0 176 11 Tx-114 tx-114 CD cord-319626-f1b3ygg0 176 12 , , , cord-319626-f1b3ygg0 176 13 and and CC cord-319626-f1b3ygg0 176 14 pooled pool VBN cord-319626-f1b3ygg0 176 15 with with IN cord-319626-f1b3ygg0 176 16 the the DT cord-319626-f1b3ygg0 176 17 labeled label VBN cord-319626-f1b3ygg0 176 18 cells cell NNS cord-319626-f1b3ygg0 176 19 that that WDT cord-319626-f1b3ygg0 176 20 were be VBD cord-319626-f1b3ygg0 176 21 also also RB cord-319626-f1b3ygg0 176 22 solubilized solubilize VBN cord-319626-f1b3ygg0 176 23 in in IN cord-319626-f1b3ygg0 176 24 Tx-114 tx-114 CD cord-319626-f1b3ygg0 176 25 . . . cord-319626-f1b3ygg0 177 1 After after IN cord-319626-f1b3ygg0 177 2 phase phase NN cord-319626-f1b3ygg0 177 3 extraction extraction NN cord-319626-f1b3ygg0 177 4 , , , cord-319626-f1b3ygg0 177 5 E1 E1 NNP cord-319626-f1b3ygg0 177 6 was be VBD cord-319626-f1b3ygg0 177 7 immunoprecipitated immunoprecipitate VBN cord-319626-f1b3ygg0 177 8 and and CC cord-319626-f1b3ygg0 177 9 resolved resolve VBN cord-319626-f1b3ygg0 177 10 by by IN cord-319626-f1b3ygg0 177 11 SDS SDS NNP cord-319626-f1b3ygg0 177 12 - - HYPH cord-319626-f1b3ygg0 177 13 PAGE PAGE NNP cord-319626-f1b3ygg0 177 14 followed follow VBN cord-319626-f1b3ygg0 177 15 by by IN cord-319626-f1b3ygg0 177 16 fluorography fluorography NNP cord-319626-f1b3ygg0 177 17 . . . cord-319626-f1b3ygg0 178 1 The the DT cord-319626-f1b3ygg0 178 2 polypeptides polypeptide NNS cord-319626-f1b3ygg0 178 3 were be VBD cord-319626-f1b3ygg0 178 4 excised excise VBN cord-319626-f1b3ygg0 178 5 from from IN cord-319626-f1b3ygg0 178 6 the the DT cord-319626-f1b3ygg0 178 7 gel gel NN cord-319626-f1b3ygg0 178 8 in in IN cord-319626-f1b3ygg0 178 9 two two CD cord-319626-f1b3ygg0 178 10 groups group NNS cord-319626-f1b3ygg0 178 11 , , , cord-319626-f1b3ygg0 178 12 the the DT cord-319626-f1b3ygg0 178 13 lower lower RBR cord-319626-f1b3ygg0 178 14 ( ( -LRB- cord-319626-f1b3ygg0 178 15 Elu Elu NNP cord-319626-f1b3ygg0 178 16 and and CC cord-319626-f1b3ygg0 178 17 Eli Eli NNP cord-319626-f1b3ygg0 178 18 ) ) -RRB- cord-319626-f1b3ygg0 178 19 and and CC cord-319626-f1b3ygg0 178 20 higher high JJR cord-319626-f1b3ygg0 178 21 ( ( -LRB- cord-319626-f1b3ygg0 178 22 Elm Elm NNP cord-319626-f1b3ygg0 178 23 ) ) -RRB- cord-319626-f1b3ygg0 178 24 molecular molecular JJ cord-319626-f1b3ygg0 178 25 mass mass NN cord-319626-f1b3ygg0 178 26 forms form NNS cord-319626-f1b3ygg0 178 27 , , , cord-319626-f1b3ygg0 178 28 and and CC cord-319626-f1b3ygg0 178 29 analyzed analyze VBN cord-319626-f1b3ygg0 178 30 by by IN cord-319626-f1b3ygg0 178 31 P4 P4 NNP cord-319626-f1b3ygg0 178 32 column column NN cord-319626-f1b3ygg0 178 33 chromatography chromatography NN cord-319626-f1b3ygg0 178 34 after after IN cord-319626-f1b3ygg0 178 35 alkaline alkaline JJ cord-319626-f1b3ygg0 178 36 hydrolysis hydrolysis NN cord-319626-f1b3ygg0 178 37 to to TO cord-319626-f1b3ygg0 178 38 release release VB cord-319626-f1b3ygg0 178 39 the the DT cord-319626-f1b3ygg0 178 40 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 178 41 side side NN cord-319626-f1b3ygg0 178 42 chains chain NNS cord-319626-f1b3ygg0 178 43 . . . cord-319626-f1b3ygg0 179 1 Any any DT cord-319626-f1b3ygg0 179 2 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 179 3 released release VBN cord-319626-f1b3ygg0 179 4 from from IN cord-319626-f1b3ygg0 179 5 the the DT cord-319626-f1b3ygg0 179 6 lower low JJR cord-319626-f1b3ygg0 179 7 molecular molecular JJ cord-319626-f1b3ygg0 179 8 mass mass NN cord-319626-f1b3ygg0 179 9 forms form NNS cord-319626-f1b3ygg0 179 10 of of IN cord-319626-f1b3ygg0 179 11 E1 E1 NNP cord-319626-f1b3ygg0 179 12 must must MD cord-319626-f1b3ygg0 179 13 have have VB cord-319626-f1b3ygg0 179 14 originated originate VBN cord-319626-f1b3ygg0 179 15 from from IN cord-319626-f1b3ygg0 179 16 Eli Eli NNP cord-319626-f1b3ygg0 179 17 since since IN cord-319626-f1b3ygg0 179 18 Elu Elu NNP cord-319626-f1b3ygg0 179 19 is be VBZ cord-319626-f1b3ygg0 179 20 not not RB cord-319626-f1b3ygg0 179 21 labeled label VBN cord-319626-f1b3ygg0 179 22 by by IN cord-319626-f1b3ygg0 179 23 [ [ -LRB- cord-319626-f1b3ygg0 179 24 3H]glucosamine 3h]glucosamine NN cord-319626-f1b3ygg0 179 25 ( ( -LRB- cord-319626-f1b3ygg0 179 26 Fig Fig NNP cord-319626-f1b3ygg0 179 27 . . NNP cord-319626-f1b3ygg0 179 28 2 2 CD cord-319626-f1b3ygg0 179 29 , , , cord-319626-f1b3ygg0 179 30 lane lane NN cord-319626-f1b3ygg0 179 31 2 2 CD cord-319626-f1b3ygg0 179 32 ) ) -RRB- cord-319626-f1b3ygg0 179 33 . . . cord-319626-f1b3ygg0 180 1 As as IN cord-319626-f1b3ygg0 180 2 shown show VBN cord-319626-f1b3ygg0 180 3 in in IN cord-319626-f1b3ygg0 180 4 Figure figure NN cord-319626-f1b3ygg0 180 5 4 4 CD cord-319626-f1b3ygg0 180 6 a a DT cord-319626-f1b3ygg0 180 7 , , , cord-319626-f1b3ygg0 180 8 a a DT cord-319626-f1b3ygg0 180 9 single single JJ cord-319626-f1b3ygg0 180 10 peak peak NN cord-319626-f1b3ygg0 180 11 of of IN cord-319626-f1b3ygg0 180 12 3H 3h CD cord-319626-f1b3ygg0 180 13 - - HYPH cord-319626-f1b3ygg0 180 14 sugar sugar NN cord-319626-f1b3ygg0 180 15 ran run VBD cord-319626-f1b3ygg0 180 16 at at IN cord-319626-f1b3ygg0 180 17 the the DT cord-319626-f1b3ygg0 180 18 position position NN cord-319626-f1b3ygg0 180 19 of of IN cord-319626-f1b3ygg0 180 20 GalNAcitol galnacitol NN cord-319626-f1b3ygg0 180 21 ( ( -LRB- cord-319626-f1b3ygg0 180 22 the the DT cord-319626-f1b3ygg0 180 23 alcohol alcohol NN cord-319626-f1b3ygg0 180 24 derivative derivative NN cord-319626-f1b3ygg0 180 25 of of IN cord-319626-f1b3ygg0 180 26 GalNAc GalNAc NNP cord-319626-f1b3ygg0 180 27 ) ) -RRB- cord-319626-f1b3ygg0 180 28 , , , cord-319626-f1b3ygg0 180 29 which which WDT cord-319626-f1b3ygg0 180 30 was be VBD cord-319626-f1b3ygg0 180 31 not not RB cord-319626-f1b3ygg0 180 32 sensitive sensitive JJ cord-319626-f1b3ygg0 180 33 to to IN cord-319626-f1b3ygg0 180 34 treatment treatment NN cord-319626-f1b3ygg0 180 35 with with IN cord-319626-f1b3ygg0 180 36 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 180 37 ( ( -LRB- cord-319626-f1b3ygg0 180 38 Fig Fig NNP cord-319626-f1b3ygg0 180 39 . . . cord-319626-f1b3ygg0 180 40 4 4 CD cord-319626-f1b3ygg0 180 41 b b LS cord-319626-f1b3ygg0 180 42 ) ) -RRB- cord-319626-f1b3ygg0 180 43 . . . cord-319626-f1b3ygg0 181 1 The the DT cord-319626-f1b3ygg0 181 2 two two CD cord-319626-f1b3ygg0 181 3 predominant predominant JJ cord-319626-f1b3ygg0 181 4 3H 3h CD cord-319626-f1b3ygg0 181 5 - - HYPH cord-319626-f1b3ygg0 181 6 labeled label VBN cord-319626-f1b3ygg0 181 7 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 181 8 from from IN cord-319626-f1b3ygg0 181 9 Elm Elm NNP cord-319626-f1b3ygg0 181 10 , , , cord-319626-f1b3ygg0 181 11 ( ( -LRB- cord-319626-f1b3ygg0 181 12 peaks peak NNS cord-319626-f1b3ygg0 181 13 Fig Fig NNP cord-319626-f1b3ygg0 181 14 . . . cord-319626-f1b3ygg0 182 1 4 4 LS cord-319626-f1b3ygg0 182 2 c c NN cord-319626-f1b3ygg0 182 3 , , , cord-319626-f1b3ygg0 182 4 A a NN cord-319626-f1b3ygg0 182 5 and and CC cord-319626-f1b3ygg0 182 6 B b NN cord-319626-f1b3ygg0 182 7 ) ) -RRB- cord-319626-f1b3ygg0 182 8 correspond correspond VB cord-319626-f1b3ygg0 182 9 to to IN cord-319626-f1b3ygg0 182 10 the the DT cord-319626-f1b3ygg0 182 11 dis!alyated dis!alyate VBN cord-319626-f1b3ygg0 182 12 and and CC cord-319626-f1b3ygg0 182 13 monosialylated monosialylate VBN cord-319626-f1b3ygg0 182 14 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 182 15 . . . cord-319626-f1b3ygg0 183 1 After after IN cord-319626-f1b3ygg0 183 2 treatment treatment NN cord-319626-f1b3ygg0 183 3 with with IN cord-319626-f1b3ygg0 183 4 neuraminidase neuraminidase NN cord-319626-f1b3ygg0 183 5 , , , cord-319626-f1b3ygg0 183 6 both both CC cord-319626-f1b3ygg0 183 7 A a NN cord-319626-f1b3ygg0 183 8 and and CC cord-319626-f1b3ygg0 183 9 B b NN cord-319626-f1b3ygg0 183 10 are be VBP cord-319626-f1b3ygg0 183 11 converted convert VBN cord-319626-f1b3ygg0 183 12 quantitatively quantitatively RB cord-319626-f1b3ygg0 183 13 to to IN cord-319626-f1b3ygg0 183 14 NANA NANA NNP cord-319626-f1b3ygg0 183 15 ( ( -LRB- cord-319626-f1b3ygg0 183 16 Fig Fig NNP cord-319626-f1b3ygg0 183 17 . . . cord-319626-f1b3ygg0 183 18 4 4 CD cord-319626-f1b3ygg0 183 19 d d NN cord-319626-f1b3ygg0 183 20 , , , cord-319626-f1b3ygg0 183 21 peak peak NNP cord-319626-f1b3ygg0 183 22 C C NNP cord-319626-f1b3ygg0 183 23 ) ) -RRB- cord-319626-f1b3ygg0 183 24 and and CC cord-319626-f1b3ygg0 183 25 Gall3(1 gall3(1 NN cord-319626-f1b3ygg0 184 1 --'3)GalNAcitol --'3)galnacitol UH cord-319626-f1b3ygg0 185 1 ( ( -LRB- cord-319626-f1b3ygg0 185 2 Fig Fig NNP cord-319626-f1b3ygg0 185 3 . . . cord-319626-f1b3ygg0 185 4 _SP cord-319626-f1b3ygg0 186 1 4 4 LS cord-319626-f1b3ygg0 186 2 d d NN cord-319626-f1b3ygg0 186 3 , , , cord-319626-f1b3ygg0 186 4 peak peak NNP cord-319626-f1b3ygg0 186 5 D D NNP cord-319626-f1b3ygg0 186 6 ) ) -RRB- cord-319626-f1b3ygg0 186 7 . . . cord-319626-f1b3ygg0 187 1 These these DT cord-319626-f1b3ygg0 187 2 sugars sugar NNS cord-319626-f1b3ygg0 187 3 , , , cord-319626-f1b3ygg0 187 4 as as RB cord-319626-f1b3ygg0 187 5 well well RB cord-319626-f1b3ygg0 187 6 as as IN cord-319626-f1b3ygg0 187 7 GalNAcitol galnacitol NN cord-319626-f1b3ygg0 188 1 ( ( -LRB- cord-319626-f1b3ygg0 188 2 Fig Fig NNP cord-319626-f1b3ygg0 188 3 . . . cord-319626-f1b3ygg0 188 4 4 4 CD cord-319626-f1b3ygg0 188 5 d d NN cord-319626-f1b3ygg0 188 6 , , , cord-319626-f1b3ygg0 188 7 peak peak NNP cord-319626-f1b3ygg0 188 8 E E NNP cord-319626-f1b3ygg0 188 9 ) ) -RRB- cord-319626-f1b3ygg0 188 10 , , , cord-319626-f1b3ygg0 188 11 are be VBP cord-319626-f1b3ygg0 188 12 present present JJ cord-319626-f1b3ygg0 188 13 in in IN cord-319626-f1b3ygg0 188 14 the the DT cord-319626-f1b3ygg0 188 15 untreated untreated JJ cord-319626-f1b3ygg0 188 16 samples sample NNS cord-319626-f1b3ygg0 188 17 ( ( -LRB- cord-319626-f1b3ygg0 188 18 Fig Fig NNP cord-319626-f1b3ygg0 188 19 . . . cord-319626-f1b3ygg0 188 20 4 4 CD cord-319626-f1b3ygg0 188 21 c c NN cord-319626-f1b3ygg0 188 22 ) ) -RRB- cord-319626-f1b3ygg0 188 23 , , , cord-319626-f1b3ygg0 188 24 most most RBS cord-319626-f1b3ygg0 188 25 likely likely JJ cord-319626-f1b3ygg0 188 26 as as IN cord-319626-f1b3ygg0 188 27 degradation degradation NN cord-319626-f1b3ygg0 188 28 products product NNS cord-319626-f1b3ygg0 188 29 of of IN cord-319626-f1b3ygg0 188 30 alkaline alkaline JJ cord-319626-f1b3ygg0 188 31 hydrolysis hydrolysis NN cord-319626-f1b3ygg0 188 32 . . . cord-319626-f1b3ygg0 189 1 Taken take VBN cord-319626-f1b3ygg0 189 2 together together RB cord-319626-f1b3ygg0 189 3 , , , cord-319626-f1b3ygg0 189 4 the the DT cord-319626-f1b3ygg0 189 5 experiments experiment NNS cord-319626-f1b3ygg0 189 6 described describe VBN cord-319626-f1b3ygg0 189 7 so so RB cord-319626-f1b3ygg0 189 8 far far RB cord-319626-f1b3ygg0 189 9 show show VBP cord-319626-f1b3ygg0 189 10 that that IN cord-319626-f1b3ygg0 189 11 GalNAc GalNAc NNP cord-319626-f1b3ygg0 189 12 is be VBZ cord-319626-f1b3ygg0 189 13 the the DT cord-319626-f1b3ygg0 189 14 first first JJ cord-319626-f1b3ygg0 189 15 sugar sugar NN cord-319626-f1b3ygg0 189 16 added add VBN cord-319626-f1b3ygg0 189 17 to to IN cord-319626-f1b3ygg0 189 18 the the DT cord-319626-f1b3ygg0 189 19 E1 E1 NNP cord-319626-f1b3ygg0 189 20 protein protein NN cord-319626-f1b3ygg0 189 21 , , , cord-319626-f1b3ygg0 189 22 followed follow VBD cord-319626-f1b3ygg0 189 23 at at IN cord-319626-f1b3ygg0 189 24 a a DT cord-319626-f1b3ygg0 189 25 later later JJ cord-319626-f1b3ygg0 189 26 time time NN cord-319626-f1b3ygg0 189 27 by by IN cord-319626-f1b3ygg0 189 28 Gal Gal NNP cord-319626-f1b3ygg0 189 29 and and CC cord-319626-f1b3ygg0 189 30 NANA NANA NNP cord-319626-f1b3ygg0 189 31 . . . cord-319626-f1b3ygg0 190 1 To to TO cord-319626-f1b3ygg0 190 2 examine examine VB cord-319626-f1b3ygg0 190 3 the the DT cord-319626-f1b3ygg0 190 4 effect effect NN cord-319626-f1b3ygg0 190 5 of of IN cord-319626-f1b3ygg0 190 6 decreased decreased JJ cord-319626-f1b3ygg0 190 7 temperature temperature NN cord-319626-f1b3ygg0 190 8 on on IN cord-319626-f1b3ygg0 190 9 the the DT cord-319626-f1b3ygg0 190 10 glycosylation glycosylation NN cord-319626-f1b3ygg0 190 11 of of IN cord-319626-f1b3ygg0 190 12 El El NNP cord-319626-f1b3ygg0 190 13 , , , cord-319626-f1b3ygg0 190 14 infected infected JJ cord-319626-f1b3ygg0 190 15 cultures culture NNS cord-319626-f1b3ygg0 190 16 were be VBD cord-319626-f1b3ygg0 190 17 labeled label VBN cord-319626-f1b3ygg0 190 18 for for IN cord-319626-f1b3ygg0 190 19 143 143 CD cord-319626-f1b3ygg0 190 20 rain rain NN cord-319626-f1b3ygg0 190 21 at at IN cord-319626-f1b3ygg0 190 22 37 37 CD cord-319626-f1b3ygg0 190 23 ° ° NN cord-319626-f1b3ygg0 190 24 C c NN cord-319626-f1b3ygg0 190 25 and and CC cord-319626-f1b3ygg0 190 26 then then RB cord-319626-f1b3ygg0 190 27 chased chase VBD cord-319626-f1b3ygg0 190 28 at at IN cord-319626-f1b3ygg0 190 29 either either DT cord-319626-f1b3ygg0 190 30 37 37 CD cord-319626-f1b3ygg0 190 31 or or CC cord-319626-f1b3ygg0 190 32 31 31 CD cord-319626-f1b3ygg0 190 33 ° ° NN cord-319626-f1b3ygg0 190 34 C c NN cord-319626-f1b3ygg0 190 35 ( ( -LRB- cord-319626-f1b3ygg0 190 36 Fig Fig NNP cord-319626-f1b3ygg0 190 37 . . . cord-319626-f1b3ygg0 190 38 5 5 CD cord-319626-f1b3ygg0 190 39 ) ) -RRB- cord-319626-f1b3ygg0 190 40 . . . cord-319626-f1b3ygg0 191 1 After after IN cord-319626-f1b3ygg0 191 2 90 90 CD cord-319626-f1b3ygg0 191 3 rain rain NN cord-319626-f1b3ygg0 191 4 of of IN cord-319626-f1b3ygg0 191 5 chase chase NN cord-319626-f1b3ygg0 191 6 at at IN cord-319626-f1b3ygg0 191 7 37 37 CD cord-319626-f1b3ygg0 191 8 ° ° NN cord-319626-f1b3ygg0 191 9 C c NN cord-319626-f1b3ygg0 191 10 , , , cord-319626-f1b3ygg0 191 11 all all DT cord-319626-f1b3ygg0 191 12 forms form NNS cord-319626-f1b3ygg0 191 13 of of IN cord-319626-f1b3ygg0 191 14 E1 e1 NN cord-319626-f1b3ygg0 191 15 were be VBD cord-319626-f1b3ygg0 191 16 detectable detectable JJ cord-319626-f1b3ygg0 191 17 in in IN cord-319626-f1b3ygg0 191 18 cell cell NN cord-319626-f1b3ygg0 191 19 extracts extract NNS cord-319626-f1b3ygg0 191 20 ( ( -LRB- cord-319626-f1b3ygg0 191 21 Fig Fig NNP cord-319626-f1b3ygg0 191 22 . . NNP cord-319626-f1b3ygg0 191 23 5 5 CD cord-319626-f1b3ygg0 191 24 , , , cord-319626-f1b3ygg0 191 25 lane lane NN cord-319626-f1b3ygg0 191 26 2 2 CD cord-319626-f1b3ygg0 191 27 ) ) -RRB- cord-319626-f1b3ygg0 191 28 . . . cord-319626-f1b3ygg0 192 1 After after IN cord-319626-f1b3ygg0 192 2 180 180 CD cord-319626-f1b3ygg0 192 3 rain rain NN cord-319626-f1b3ygg0 192 4 there there EX cord-319626-f1b3ygg0 192 5 was be VBD cord-319626-f1b3ygg0 192 6 a a DT cord-319626-f1b3ygg0 192 7 decrease decrease NN cord-319626-f1b3ygg0 192 8 in in IN cord-319626-f1b3ygg0 192 9 the the DT cord-319626-f1b3ygg0 192 10 amount amount NN cord-319626-f1b3ygg0 192 11 of of IN cord-319626-f1b3ygg0 192 12 all all DT cord-319626-f1b3ygg0 192 13 forms form NNS cord-319626-f1b3ygg0 192 14 of of IN cord-319626-f1b3ygg0 192 15 labeled label VBN cord-319626-f1b3ygg0 192 16 E1 e1 NN cord-319626-f1b3ygg0 192 17 ( ( -LRB- cord-319626-f1b3ygg0 192 18 Fig Fig NNP cord-319626-f1b3ygg0 192 19 . . NNP cord-319626-f1b3ygg0 192 20 5 5 CD cord-319626-f1b3ygg0 192 21 , , , cord-319626-f1b3ygg0 192 22 lane lane NN cord-319626-f1b3ygg0 192 23 _SP cord-319626-f1b3ygg0 192 24 3 3 CD cord-319626-f1b3ygg0 192 25 ) ) -RRB- cord-319626-f1b3ygg0 192 26 , , , cord-319626-f1b3ygg0 192 27 presumably presumably RB cord-319626-f1b3ygg0 192 28 because because IN cord-319626-f1b3ygg0 192 29 the the DT cord-319626-f1b3ygg0 192 30 labeled label VBN cord-319626-f1b3ygg0 192 31 E1 e1 NN cord-319626-f1b3ygg0 192 32 molecules molecule NNS cord-319626-f1b3ygg0 192 33 had have VBD cord-319626-f1b3ygg0 192 34 been be VBN cord-319626-f1b3ygg0 192 35 incorporated incorporate VBN cord-319626-f1b3ygg0 192 36 into into IN cord-319626-f1b3ygg0 192 37 virions virion NNS cord-319626-f1b3ygg0 192 38 which which WDT cord-319626-f1b3ygg0 192 39 were be VBD cord-319626-f1b3ygg0 192 40 then then RB cord-319626-f1b3ygg0 192 41 transported transport VBN cord-319626-f1b3ygg0 192 42 out out IN cord-319626-f1b3ygg0 192 43 of of IN cord-319626-f1b3ygg0 192 44 the the DT cord-319626-f1b3ygg0 192 45 cell cell NN cord-319626-f1b3ygg0 192 46 . . . cord-319626-f1b3ygg0 193 1 When when WRB cord-319626-f1b3ygg0 193 2 the the DT cord-319626-f1b3ygg0 193 3 cultures culture NNS cord-319626-f1b3ygg0 193 4 were be VBD cord-319626-f1b3ygg0 193 5 shifted shift VBN cord-319626-f1b3ygg0 193 6 to to IN cord-319626-f1b3ygg0 193 7 31 31 CD cord-319626-f1b3ygg0 193 8 ° ° NN cord-319626-f1b3ygg0 193 9 C c NN cord-319626-f1b3ygg0 193 10 for for IN cord-319626-f1b3ygg0 193 11 the the DT cord-319626-f1b3ygg0 193 12 chase chase NN cord-319626-f1b3ygg0 193 13 period period NN cord-319626-f1b3ygg0 193 14 , , , cord-319626-f1b3ygg0 193 15 the the DT cord-319626-f1b3ygg0 193 16 glycosylation glycosylation NN cord-319626-f1b3ygg0 193 17 of of IN cord-319626-f1b3ygg0 193 18 E1 e1 NN cord-319626-f1b3ygg0 193 19 was be VBD cord-319626-f1b3ygg0 193 20 blocked block VBN cord-319626-f1b3ygg0 193 21 in in IN cord-319626-f1b3ygg0 193 22 the the DT cord-319626-f1b3ygg0 193 23 intermediate intermediate JJ cord-319626-f1b3ygg0 193 24 form form NN cord-319626-f1b3ygg0 193 25 ( ( -LRB- cord-319626-f1b3ygg0 193 26 Fig Fig NNP cord-319626-f1b3ygg0 193 27 . . NNP cord-319626-f1b3ygg0 193 28 5 5 CD cord-319626-f1b3ygg0 193 29 , , , cord-319626-f1b3ygg0 193 30 lanes lane NNS cord-319626-f1b3ygg0 193 31 4 4 CD cord-319626-f1b3ygg0 193 32 and and CC cord-319626-f1b3ygg0 193 33 5 5 CD cord-319626-f1b3ygg0 193 34 ) ) -RRB- cord-319626-f1b3ygg0 193 35 . . . cord-319626-f1b3ygg0 194 1 This this DT cord-319626-f1b3ygg0 194 2 block block NN cord-319626-f1b3ygg0 194 3 was be VBD cord-319626-f1b3ygg0 194 4 maintained maintain VBN cord-319626-f1b3ygg0 194 5 after after IN cord-319626-f1b3ygg0 194 6 180 180 CD cord-319626-f1b3ygg0 194 7 min min NN cord-319626-f1b3ygg0 194 8 of of IN cord-319626-f1b3ygg0 194 9 chase chase NN cord-319626-f1b3ygg0 194 10 , , , cord-319626-f1b3ygg0 194 11 with with IN cord-319626-f1b3ygg0 194 12 no no DT cord-319626-f1b3ygg0 194 13 detectable detectable JJ cord-319626-f1b3ygg0 194 14 decrease decrease NN cord-319626-f1b3ygg0 194 15 in in IN cord-319626-f1b3ygg0 194 16 either either CC cord-319626-f1b3ygg0 194 17 the the DT cord-319626-f1b3ygg0 194 18 unglycosylated unglycosylated JJ cord-319626-f1b3ygg0 194 19 or or CC cord-319626-f1b3ygg0 194 20 the the DT cord-319626-f1b3ygg0 194 21 intermediate intermediate JJ cord-319626-f1b3ygg0 194 22 form form NN cord-319626-f1b3ygg0 194 23 ( ( -LRB- cord-319626-f1b3ygg0 194 24 Fig Fig NNP cord-319626-f1b3ygg0 194 25 . . NNP cord-319626-f1b3ygg0 194 26 5 5 CD cord-319626-f1b3ygg0 194 27 , , , cord-319626-f1b3ygg0 194 28 compare compare VB cord-319626-f1b3ygg0 194 29 lanes lane NNS cord-319626-f1b3ygg0 194 30 2 2 CD cord-319626-f1b3ygg0 194 31 and and CC cord-319626-f1b3ygg0 194 32 4 4 CD cord-319626-f1b3ygg0 194 33 with with IN cord-319626-f1b3ygg0 194 34 lanes lane NNS cord-319626-f1b3ygg0 194 35 3 3 CD cord-319626-f1b3ygg0 194 36 and and CC cord-319626-f1b3ygg0 194 37 5 5 CD cord-319626-f1b3ygg0 194 38 ) ) -RRB- cord-319626-f1b3ygg0 194 39 . . . cord-319626-f1b3ygg0 195 1 If if IN cord-319626-f1b3ygg0 195 2 , , , cord-319626-f1b3ygg0 195 3 after after IN cord-319626-f1b3ygg0 195 4 90 90 CD cord-319626-f1b3ygg0 195 5 min min NN cord-319626-f1b3ygg0 195 6 of of IN cord-319626-f1b3ygg0 195 7 chase chase NNP cord-319626-f1b3ygg0 195 8 at at IN cord-319626-f1b3ygg0 195 9 31 31 CD cord-319626-f1b3ygg0 195 10 ° ° NN cord-319626-f1b3ygg0 195 11 C c NN cord-319626-f1b3ygg0 195 12 , , , cord-319626-f1b3ygg0 195 13 the the DT cord-319626-f1b3ygg0 195 14 infected infected JJ cord-319626-f1b3ygg0 195 15 cultures culture NNS cord-319626-f1b3ygg0 195 16 were be VBD cord-319626-f1b3ygg0 195 17 returned return VBN cord-319626-f1b3ygg0 195 18 to to IN cord-319626-f1b3ygg0 195 19 37 37 CD cord-319626-f1b3ygg0 195 20 ° ° NN cord-319626-f1b3ygg0 195 21 C c NN cord-319626-f1b3ygg0 195 22 for for IN cord-319626-f1b3ygg0 195 23 a a DT cord-319626-f1b3ygg0 195 24 further further JJ cord-319626-f1b3ygg0 195 25 90 90 CD cord-319626-f1b3ygg0 195 26 min min NN cord-319626-f1b3ygg0 195 27 , , , cord-319626-f1b3ygg0 195 28 the the DT cord-319626-f1b3ygg0 195 29 amounts amount NNS cord-319626-f1b3ygg0 195 30 of of IN cord-319626-f1b3ygg0 195 31 the the DT cord-319626-f1b3ygg0 195 32 Elu Elu NNP cord-319626-f1b3ygg0 195 33 and and CC cord-319626-f1b3ygg0 195 34 Eli Eli NNP cord-319626-f1b3ygg0 195 35 forms form NNS cord-319626-f1b3ygg0 195 36 decreased decrease VBD cord-319626-f1b3ygg0 195 37 ( ( -LRB- cord-319626-f1b3ygg0 195 38 Fig Fig NNP cord-319626-f1b3ygg0 195 39 . . NNP cord-319626-f1b3ygg0 195 40 5 5 CD cord-319626-f1b3ygg0 195 41 , , , cord-319626-f1b3ygg0 195 42 1 1 CD cord-319626-f1b3ygg0 195 43 and and CC cord-319626-f1b3ygg0 195 44 2 2 CD cord-319626-f1b3ygg0 195 45 with with IN cord-319626-f1b3ygg0 195 46 lanes lane NNS cord-319626-f1b3ygg0 195 47 4 4 CD cord-319626-f1b3ygg0 195 48 and and CC cord-319626-f1b3ygg0 195 49 6 6 CD cord-319626-f1b3ygg0 195 50 ) ) -RRB- cord-319626-f1b3ygg0 195 51 . . . cord-319626-f1b3ygg0 195 52 .~ .~ . cord-319626-f1b3ygg0 196 1 2o 2o CD cord-319626-f1b3ygg0 196 2 forms form NNS cord-319626-f1b3ygg0 196 3 of of IN cord-319626-f1b3ygg0 196 4 El El NNP cord-319626-f1b3ygg0 196 5 were be VBD cord-319626-f1b3ygg0 196 6 excised excise VBN cord-319626-f1b3ygg0 196 7 from from IN cord-319626-f1b3ygg0 196 8 the the DT cord-319626-f1b3ygg0 196 9 gel gel NN cord-319626-f1b3ygg0 196 10 and and CC cord-319626-f1b3ygg0 196 11 quantitated quantitated JJ cord-319626-f1b3ygg0 196 12 ; ; : cord-319626-f1b3ygg0 196 13 the the DT cord-319626-f1b3ygg0 196 14 results result NNS cord-319626-f1b3ygg0 196 15 are be VBP cord-319626-f1b3ygg0 196 16 presented present VBN cord-319626-f1b3ygg0 196 17 in in IN cord-319626-f1b3ygg0 196 18 Fig Fig NNP cord-319626-f1b3ygg0 196 19 . . . cord-319626-f1b3ygg0 197 1 6 6 CD cord-319626-f1b3ygg0 197 2 a. a. NN cord-319626-f1b3ygg0 198 1 As as IN cord-319626-f1b3ygg0 198 2 expected expect VBN cord-319626-f1b3ygg0 198 3 from from IN cord-319626-f1b3ygg0 198 4 Fig Fig NNP cord-319626-f1b3ygg0 198 5 . . . cord-319626-f1b3ygg0 199 1 5 5 LS cord-319626-f1b3ygg0 199 2 , , , cord-319626-f1b3ygg0 199 3 a a DT cord-319626-f1b3ygg0 199 4 decrease decrease NN cord-319626-f1b3ygg0 199 5 in in IN cord-319626-f1b3ygg0 199 6 the the DT cord-319626-f1b3ygg0 199 7 incubation incubation NN cord-319626-f1b3ygg0 199 8 temperature temperature NN cord-319626-f1b3ygg0 199 9 reduces reduce VBZ cord-319626-f1b3ygg0 199 10 the the DT cord-319626-f1b3ygg0 199 11 percentage percentage NN cord-319626-f1b3ygg0 199 12 of of IN cord-319626-f1b3ygg0 199 13 E1 E1 NNP cord-319626-f1b3ygg0 199 14 m m NNP cord-319626-f1b3ygg0 199 15 and and CC cord-319626-f1b3ygg0 199 16 increases increase VBZ cord-319626-f1b3ygg0 199 17 the the DT cord-319626-f1b3ygg0 199 18 percentage percentage NN cord-319626-f1b3ygg0 199 19 of of IN cord-319626-f1b3ygg0 199 20 E1 e1 NN cord-319626-f1b3ygg0 200 1 o. o. NNP cord-319626-f1b3ygg0 200 2 Surprisingly Surprisingly NNP cord-319626-f1b3ygg0 200 3 , , , cord-319626-f1b3ygg0 200 4 the the DT cord-319626-f1b3ygg0 200 5 percentage percentage NN cord-319626-f1b3ygg0 200 6 of of IN cord-319626-f1b3ygg0 200 7 EI~ EI~ NNP cord-319626-f1b3ygg0 200 8 remains remain VBZ cord-319626-f1b3ygg0 200 9 approximately approximately RB cord-319626-f1b3ygg0 200 10 constant constant JJ cord-319626-f1b3ygg0 200 11 at at IN cord-319626-f1b3ygg0 200 12 all all PDT cord-319626-f1b3ygg0 200 13 the the DT cord-319626-f1b3ygg0 200 14 temperatures temperature NNS cord-319626-f1b3ygg0 200 15 . . . cord-319626-f1b3ygg0 201 1 In in IN cord-319626-f1b3ygg0 201 2 a a DT cord-319626-f1b3ygg0 201 3 separate separate JJ cord-319626-f1b3ygg0 201 4 experiment experiment NN cord-319626-f1b3ygg0 201 5 the the DT cord-319626-f1b3ygg0 201 6 absolute absolute JJ cord-319626-f1b3ygg0 201 7 amounts amount NNS cord-319626-f1b3ygg0 201 8 of of IN cord-319626-f1b3ygg0 201 9 each each DT cord-319626-f1b3ygg0 201 10 of of IN cord-319626-f1b3ygg0 201 11 the the DT cord-319626-f1b3ygg0 201 12 E1 e1 NN cord-319626-f1b3ygg0 201 13 forms form NNS cord-319626-f1b3ygg0 201 14 were be VBD cord-319626-f1b3ygg0 201 15 measured measure VBN cord-319626-f1b3ygg0 201 16 at at IN cord-319626-f1b3ygg0 201 17 both both DT cord-319626-f1b3ygg0 201 18 37 37 CD cord-319626-f1b3ygg0 201 19 and and CC cord-319626-f1b3ygg0 201 20 31 31 CD cord-319626-f1b3ygg0 201 21 ° ° NN cord-319626-f1b3ygg0 201 22 C c NN cord-319626-f1b3ygg0 201 23 and and CC cord-319626-f1b3ygg0 201 24 , , , cord-319626-f1b3ygg0 201 25 as as IN cord-319626-f1b3ygg0 201 26 shown show VBN cord-319626-f1b3ygg0 201 27 in in IN cord-319626-f1b3ygg0 201 28 Fig Fig NNP cord-319626-f1b3ygg0 201 29 . . . cord-319626-f1b3ygg0 202 1 6 6 CD cord-319626-f1b3ygg0 202 2 b b NN cord-319626-f1b3ygg0 202 3 , , , cord-319626-f1b3ygg0 202 4 the the DT cord-319626-f1b3ygg0 202 5 decrease decrease NN cord-319626-f1b3ygg0 202 6 in in IN cord-319626-f1b3ygg0 202 7 the the DT cord-319626-f1b3ygg0 202 8 mature mature JJ cord-319626-f1b3ygg0 202 9 forms form NNS cord-319626-f1b3ygg0 202 10 , , , cord-319626-f1b3ygg0 202 11 Elm Elm NNP cord-319626-f1b3ygg0 202 12 , , , cord-319626-f1b3ygg0 202 13 was be VBD cord-319626-f1b3ygg0 202 14 accompanied accompany VBN cord-319626-f1b3ygg0 202 15 by by IN cord-319626-f1b3ygg0 202 16 an an DT cord-319626-f1b3ygg0 202 17 increase increase NN cord-319626-f1b3ygg0 202 18 in in IN cord-319626-f1b3ygg0 202 19 the the DT cord-319626-f1b3ygg0 202 20 unglycosylated unglycosylated JJ cord-319626-f1b3ygg0 202 21 form form NN cord-319626-f1b3ygg0 202 22 , , , cord-319626-f1b3ygg0 202 23 Elo Elo NNP cord-319626-f1b3ygg0 202 24 , , , cord-319626-f1b3ygg0 202 25 but but CC cord-319626-f1b3ygg0 202 26 there there EX cord-319626-f1b3ygg0 202 27 was be VBD cord-319626-f1b3ygg0 202 28 no no DT cord-319626-f1b3ygg0 202 29 change change NN cord-319626-f1b3ygg0 202 30 in in IN cord-319626-f1b3ygg0 202 31 the the DT cord-319626-f1b3ygg0 202 32 amount amount NN cord-319626-f1b3ygg0 202 33 of of IN cord-319626-f1b3ygg0 202 34 the the DT cord-319626-f1b3ygg0 202 35 intermediate intermediate JJ cord-319626-f1b3ygg0 202 36 form form NN cord-319626-f1b3ygg0 202 37 , , , cord-319626-f1b3ygg0 202 38 EI~. EI~. NNP cord-319626-f1b3ygg0 203 1 To to TO cord-319626-f1b3ygg0 203 2 relate relate VB cord-319626-f1b3ygg0 203 3 the the DT cord-319626-f1b3ygg0 203 4 block block NN cord-319626-f1b3ygg0 203 5 in in IN cord-319626-f1b3ygg0 203 6 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 203 7 maturation maturation NN cord-319626-f1b3ygg0 203 8 to to IN cord-319626-f1b3ygg0 203 9 the the DT cord-319626-f1b3ygg0 203 10 intracellular intracellular JJ cord-319626-f1b3ygg0 203 11 localization localization NN cord-319626-f1b3ygg0 203 12 of of IN cord-319626-f1b3ygg0 203 13 El El NNP cord-319626-f1b3ygg0 203 14 , , , cord-319626-f1b3ygg0 203 15 infected infected JJ cord-319626-f1b3ygg0 203 16 cells cell NNS cord-319626-f1b3ygg0 203 17 were be VBD cord-319626-f1b3ygg0 203 18 labeled label VBN cord-319626-f1b3ygg0 203 19 for for IN cord-319626-f1b3ygg0 203 20 indirect indirect JJ cord-319626-f1b3ygg0 203 21 immunofluorescence immunofluorescence NN cord-319626-f1b3ygg0 203 22 microscopy microscopy NN cord-319626-f1b3ygg0 203 23 , , , cord-319626-f1b3ygg0 203 24 using use VBG cord-319626-f1b3ygg0 203 25 a a DT cord-319626-f1b3ygg0 203 26 mAb mab NN cord-319626-f1b3ygg0 203 27 against against IN cord-319626-f1b3ygg0 203 28 El El NNP cord-319626-f1b3ygg0 203 29 . . . cord-319626-f1b3ygg0 204 1 At at IN cord-319626-f1b3ygg0 204 2 7 7 CD cord-319626-f1b3ygg0 204 3 h h NN cord-319626-f1b3ygg0 204 4 after after IN cord-319626-f1b3ygg0 204 5 infection infection NN cord-319626-f1b3ygg0 204 6 at at IN cord-319626-f1b3ygg0 204 7 37 37 CD cord-319626-f1b3ygg0 204 8 ° ° NN cord-319626-f1b3ygg0 204 9 C c NN cord-319626-f1b3ygg0 204 10 , , , cord-319626-f1b3ygg0 204 11 E1 E1 NNP cord-319626-f1b3ygg0 204 12 was be VBD cord-319626-f1b3ygg0 204 13 restricted restrict VBN cord-319626-f1b3ygg0 204 14 to to IN cord-319626-f1b3ygg0 204 15 a a DT cord-319626-f1b3ygg0 204 16 perinuclear perinuclear JJ cord-319626-f1b3ygg0 204 17 region region NN cord-319626-f1b3ygg0 204 18 ( ( -LRB- cord-319626-f1b3ygg0 204 19 Fig Fig NNP cord-319626-f1b3ygg0 204 20 . . . cord-319626-f1b3ygg0 204 21 7 7 CD cord-319626-f1b3ygg0 204 22 a a LS cord-319626-f1b3ygg0 204 23 ) ) -RRB- cord-319626-f1b3ygg0 204 24 and and CC cord-319626-f1b3ygg0 204 25 the the DT cord-319626-f1b3ygg0 204 26 pattern pattern NN cord-319626-f1b3ygg0 204 27 of of IN cord-319626-f1b3ygg0 204 28 labeling labeling NN cord-319626-f1b3ygg0 204 29 was be VBD cord-319626-f1b3ygg0 204 30 similar similar JJ cord-319626-f1b3ygg0 204 31 to to IN cord-319626-f1b3ygg0 204 32 , , , cord-319626-f1b3ygg0 204 33 but but CC cord-319626-f1b3ygg0 204 34 more more RBR cord-319626-f1b3ygg0 204 35 extensive extensive JJ cord-319626-f1b3ygg0 204 36 than than IN cord-319626-f1b3ygg0 204 37 , , , cord-319626-f1b3ygg0 204 38 that that DT cord-319626-f1b3ygg0 204 39 observed observe VBD cord-319626-f1b3ygg0 204 40 with with IN cord-319626-f1b3ygg0 204 41 antibodies antibody NNS cord-319626-f1b3ygg0 204 42 specific specific JJ cord-319626-f1b3ygg0 204 43 for for IN cord-319626-f1b3ygg0 204 44 an an DT cord-319626-f1b3ygg0 204 45 endogenous endogenous JJ cord-319626-f1b3ygg0 204 46 Golgi Golgi NNP cord-319626-f1b3ygg0 204 47 protein protein NN cord-319626-f1b3ygg0 204 48 ( ( -LRB- cord-319626-f1b3ygg0 204 49 data datum NNS cord-319626-f1b3ygg0 204 50 not not RB cord-319626-f1b3ygg0 204 51 shown show VBN cord-319626-f1b3ygg0 204 52 ) ) -RRB- cord-319626-f1b3ygg0 204 53 . . . cord-319626-f1b3ygg0 205 1 Between between IN cord-319626-f1b3ygg0 205 2 7 7 CD cord-319626-f1b3ygg0 205 3 and and CC cord-319626-f1b3ygg0 205 4 12 12 CD cord-319626-f1b3ygg0 205 5 h h NN cord-319626-f1b3ygg0 205 6 after after IN cord-319626-f1b3ygg0 205 7 infection infection NN cord-319626-f1b3ygg0 205 8 at at IN cord-319626-f1b3ygg0 205 9 37 37 CD cord-319626-f1b3ygg0 205 10 ° ° NN cord-319626-f1b3ygg0 205 11 C c NN cord-319626-f1b3ygg0 205 12 syncytia syncytia NN cord-319626-f1b3ygg0 205 13 developed develop VBN cord-319626-f1b3ygg0 205 14 , , , cord-319626-f1b3ygg0 205 15 each each DT cord-319626-f1b3ygg0 205 16 containing contain VBG cord-319626-f1b3ygg0 205 17 several several JJ cord-319626-f1b3ygg0 205 18 hundred hundred CD cord-319626-f1b3ygg0 205 19 nuclei nucleus NNS cord-319626-f1b3ygg0 205 20 , , , cord-319626-f1b3ygg0 205 21 and and CC cord-319626-f1b3ygg0 205 22 concomitantly concomitantly RB cord-319626-f1b3ygg0 205 23 E1 E1 NNP cord-319626-f1b3ygg0 205 24 became become VBD cord-319626-f1b3ygg0 205 25 detectable detectable JJ cord-319626-f1b3ygg0 205 26 in in IN cord-319626-f1b3ygg0 205 27 the the DT cord-319626-f1b3ygg0 205 28 rough rough JJ cord-319626-f1b3ygg0 205 29 ER er NN cord-319626-f1b3ygg0 205 30 throughout throughout IN cord-319626-f1b3ygg0 205 31 the the DT cord-319626-f1b3ygg0 205 32 cytoplasm cytoplasm NN cord-319626-f1b3ygg0 206 1 ( ( -LRB- cord-319626-f1b3ygg0 206 2 Fig Fig NNP cord-319626-f1b3ygg0 206 3 . . NNP cord-319626-f1b3ygg0 206 4 7 7 CD cord-319626-f1b3ygg0 206 5 b b NN cord-319626-f1b3ygg0 206 6 ) ) -RRB- cord-319626-f1b3ygg0 206 7 . . . cord-319626-f1b3ygg0 207 1 At at IN cord-319626-f1b3ygg0 207 2 these these DT cord-319626-f1b3ygg0 207 3 late late JJ cord-319626-f1b3ygg0 207 4 times time NNS cord-319626-f1b3ygg0 207 5 the the DT cord-319626-f1b3ygg0 207 6 mAb mAb NNS cord-319626-f1b3ygg0 207 7 against against IN cord-319626-f1b3ygg0 207 8 E1 E1 NNP cord-319626-f1b3ygg0 207 9 also also RB cord-319626-f1b3ygg0 207 10 labeled label VBD cord-319626-f1b3ygg0 207 11 patches patch NNS cord-319626-f1b3ygg0 207 12 at at IN cord-319626-f1b3ygg0 207 13 the the DT cord-319626-f1b3ygg0 207 14 cell cell NN cord-319626-f1b3ygg0 207 15 surface surface NN cord-319626-f1b3ygg0 207 16 ( ( -LRB- cord-319626-f1b3ygg0 207 17 Fig Fig NNP cord-319626-f1b3ygg0 207 18 . . NNP cord-319626-f1b3ygg0 207 19 7 7 CD cord-319626-f1b3ygg0 207 20 b b NN cord-319626-f1b3ygg0 207 21 ) ) -RRB- cord-319626-f1b3ygg0 207 22 ; ; : cord-319626-f1b3ygg0 207 23 this this DT cord-319626-f1b3ygg0 207 24 labeling labeling NN cord-319626-f1b3ygg0 207 25 we -PRON- PRP cord-319626-f1b3ygg0 207 26 attribute attribute VBP cord-319626-f1b3ygg0 207 27 to to IN cord-319626-f1b3ygg0 207 28 released release VBN cord-319626-f1b3ygg0 207 29 progeny progeny JJ cord-319626-f1b3ygg0 207 30 virions virion NNS cord-319626-f1b3ygg0 207 31 , , , cord-319626-f1b3ygg0 207 32 which which WDT cord-319626-f1b3ygg0 207 33 are be VBP cord-319626-f1b3ygg0 207 34 known know VBN cord-319626-f1b3ygg0 207 35 to to TO cord-319626-f1b3ygg0 207 36 remain remain VB cord-319626-f1b3ygg0 207 37 closely closely RB cord-319626-f1b3ygg0 207 38 associated associate VBN cord-319626-f1b3ygg0 207 39 with with IN cord-319626-f1b3ygg0 207 40 the the DT cord-319626-f1b3ygg0 207 41 cell cell NN cord-319626-f1b3ygg0 207 42 surface surface NN cord-319626-f1b3ygg0 208 1 ( ( -LRB- cord-319626-f1b3ygg0 208 2 Dubois Dubois NNP cord-319626-f1b3ygg0 208 3 - - HYPH cord-319626-f1b3ygg0 208 4 Dalcq Dalcq NNP cord-319626-f1b3ygg0 208 5 et et NNP cord-319626-f1b3ygg0 208 6 al al NNP cord-319626-f1b3ygg0 208 7 . . NNP cord-319626-f1b3ygg0 208 8 , , , cord-319626-f1b3ygg0 208 9 1984 1984 CD cord-319626-f1b3ygg0 208 10 ) ) -RRB- cord-319626-f1b3ygg0 208 11 . . . cord-319626-f1b3ygg0 209 1 In in IN cord-319626-f1b3ygg0 209 2 contrast contrast NN cord-319626-f1b3ygg0 209 3 , , , cord-319626-f1b3ygg0 209 4 when when WRB cord-319626-f1b3ygg0 209 5 the the DT cord-319626-f1b3ygg0 209 6 cells cell NNS cord-319626-f1b3ygg0 209 7 were be VBD cord-319626-f1b3ygg0 209 8 shifted shift VBN cord-319626-f1b3ygg0 209 9 at at IN cord-319626-f1b3ygg0 209 10 7 7 CD cord-319626-f1b3ygg0 209 11 h h NN cord-319626-f1b3ygg0 209 12 after after IN cord-319626-f1b3ygg0 209 13 infection infection NN cord-319626-f1b3ygg0 209 14 to to IN cord-319626-f1b3ygg0 209 15 31 31 CD cord-319626-f1b3ygg0 209 16 ° ° NN cord-319626-f1b3ygg0 209 17 C c NN cord-319626-f1b3ygg0 209 18 and and CC cord-319626-f1b3ygg0 209 19 incubated incubate VBN cord-319626-f1b3ygg0 209 20 until until IN cord-319626-f1b3ygg0 209 21 12 12 CD cord-319626-f1b3ygg0 209 22 h h NN cord-319626-f1b3ygg0 209 23 after after IN cord-319626-f1b3ygg0 209 24 infection infection NN cord-319626-f1b3ygg0 209 25 , , , cord-319626-f1b3ygg0 209 26 the the DT cord-319626-f1b3ygg0 209 27 E1 e1 NN cord-319626-f1b3ygg0 209 28 labeling labeling NN cord-319626-f1b3ygg0 209 29 within within IN cord-319626-f1b3ygg0 209 30 the the DT cord-319626-f1b3ygg0 209 31 cells cell NNS cord-319626-f1b3ygg0 209 32 remained remain VBD cord-319626-f1b3ygg0 209 33 restricted restricted JJ cord-319626-f1b3ygg0 209 34 to to IN cord-319626-f1b3ygg0 209 35 the the DT cord-319626-f1b3ygg0 209 36 peri peri NNP cord-319626-f1b3ygg0 209 37 - - HYPH cord-319626-f1b3ygg0 209 38 Golgi Golgi NNP cord-319626-f1b3ygg0 209 39 region region NN cord-319626-f1b3ygg0 209 40 , , , cord-319626-f1b3ygg0 209 41 although although IN cord-319626-f1b3ygg0 209 42 the the DT cord-319626-f1b3ygg0 209 43 area area NN cord-319626-f1b3ygg0 209 44 and and CC cord-319626-f1b3ygg0 209 45 intensity intensity NN cord-319626-f1b3ygg0 209 46 of of IN cord-319626-f1b3ygg0 209 47 labeling labeling NN cord-319626-f1b3ygg0 209 48 increased increase VBD cord-319626-f1b3ygg0 209 49 above above IN cord-319626-f1b3ygg0 209 50 that that DT cord-319626-f1b3ygg0 209 51 seen see VBN cord-319626-f1b3ygg0 209 52 at at IN cord-319626-f1b3ygg0 209 53 7 7 CD cord-319626-f1b3ygg0 209 54 h h NN cord-319626-f1b3ygg0 209 55 after after IN cord-319626-f1b3ygg0 209 56 infection infection NN cord-319626-f1b3ygg0 209 57 ( ( -LRB- cord-319626-f1b3ygg0 209 58 Fig Fig NNP cord-319626-f1b3ygg0 209 59 . . NNP cord-319626-f1b3ygg0 209 60 7 7 CD cord-319626-f1b3ygg0 209 61 , , , cord-319626-f1b3ygg0 209 62 a a NN cord-319626-f1b3ygg0 209 63 and and CC cord-319626-f1b3ygg0 209 64 c c NN cord-319626-f1b3ygg0 209 65 ) ) -RRB- cord-319626-f1b3ygg0 209 66 . . . cord-319626-f1b3ygg0 210 1 Furthermore furthermore RB cord-319626-f1b3ygg0 210 2 , , , cord-319626-f1b3ygg0 210 3 neither neither CC cord-319626-f1b3ygg0 210 4 the the DT cord-319626-f1b3ygg0 210 5 rough rough JJ cord-319626-f1b3ygg0 210 6 ER er NN cord-319626-f1b3ygg0 210 7 nor nor CC cord-319626-f1b3ygg0 210 8 the the DT cord-319626-f1b3ygg0 210 9 surface surface NN cord-319626-f1b3ygg0 210 10 of of IN cord-319626-f1b3ygg0 210 11 cells cell NNS cord-319626-f1b3ygg0 210 12 incubated incubate VBN cord-319626-f1b3ygg0 210 13 from from IN cord-319626-f1b3ygg0 210 14 7 7 CD cord-319626-f1b3ygg0 210 15 to to TO cord-319626-f1b3ygg0 210 16 12 12 CD cord-319626-f1b3ygg0 210 17 h h NN cord-319626-f1b3ygg0 210 18 after after IN cord-319626-f1b3ygg0 210 19 infection infection NN cord-319626-f1b3ygg0 210 20 at at IN cord-319626-f1b3ygg0 210 21 31 31 CD cord-319626-f1b3ygg0 210 22 ° ° NN cord-319626-f1b3ygg0 210 23 C C NNP cord-319626-f1b3ygg0 210 24 labeled label VBN cord-319626-f1b3ygg0 210 25 for for IN cord-319626-f1b3ygg0 210 26 El El NNP cord-319626-f1b3ygg0 210 27 . . . cord-319626-f1b3ygg0 211 1 These these DT cord-319626-f1b3ygg0 211 2 observations observation NNS cord-319626-f1b3ygg0 211 3 imply imply VBP cord-319626-f1b3ygg0 211 4 that that IN cord-319626-f1b3ygg0 211 5 E1 E1 NNP cord-319626-f1b3ygg0 211 6 accumulated accumulate VBN cord-319626-f1b3ygg0 211 7 in in IN cord-319626-f1b3ygg0 211 8 the the DT cord-319626-f1b3ygg0 211 9 perinuclear perinuclear JJ cord-319626-f1b3ygg0 211 10 region region NN cord-319626-f1b3ygg0 211 11 during during IN cord-319626-f1b3ygg0 211 12 the the DT cord-319626-f1b3ygg0 211 13 incubation incubation NN cord-319626-f1b3ygg0 211 14 at at IN cord-319626-f1b3ygg0 211 15 31 31 CD cord-319626-f1b3ygg0 211 16 ° ° NN cord-319626-f1b3ygg0 211 17 C c NN cord-319626-f1b3ygg0 211 18 and and CC cord-319626-f1b3ygg0 211 19 that that IN cord-319626-f1b3ygg0 211 20 virions virion NNS cord-319626-f1b3ygg0 211 21 were be VBD cord-319626-f1b3ygg0 211 22 not not RB cord-319626-f1b3ygg0 211 23 transported transport VBN cord-319626-f1b3ygg0 211 24 out out IN cord-319626-f1b3ygg0 211 25 of of IN cord-319626-f1b3ygg0 211 26 the the DT cord-319626-f1b3ygg0 211 27 cell cell NN cord-319626-f1b3ygg0 211 28 . . . cord-319626-f1b3ygg0 212 1 During during IN cord-319626-f1b3ygg0 212 2 the the DT cord-319626-f1b3ygg0 212 3 5-h 5-h CD cord-319626-f1b3ygg0 212 4 incubation incubation NN cord-319626-f1b3ygg0 212 5 at at IN cord-319626-f1b3ygg0 212 6 31 31 CD cord-319626-f1b3ygg0 212 7 ° ° NN cord-319626-f1b3ygg0 212 8 C C NNP cord-319626-f1b3ygg0 212 9 , , , cord-319626-f1b3ygg0 212 10 the the DT cord-319626-f1b3ygg0 212 11 syncitia syncitia NN cord-319626-f1b3ygg0 212 12 also also RB cord-319626-f1b3ygg0 212 13 failed fail VBD cord-319626-f1b3ygg0 212 14 to to TO cord-319626-f1b3ygg0 212 15 enlarge enlarge VB cord-319626-f1b3ygg0 212 16 significantly significantly RB cord-319626-f1b3ygg0 212 17 , , , cord-319626-f1b3ygg0 212 18 The the DT cord-319626-f1b3ygg0 212 19 viral viral JJ cord-319626-f1b3ygg0 212 20 envelope envelope NN cord-319626-f1b3ygg0 212 21 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 212 22 E2 E2 NNP cord-319626-f1b3ygg0 212 23 is be VBZ cord-319626-f1b3ygg0 212 24 responsible responsible JJ cord-319626-f1b3ygg0 212 25 for for IN cord-319626-f1b3ygg0 212 26 viral viral RB cord-319626-f1b3ygg0 212 27 - - HYPH cord-319626-f1b3ygg0 212 28 induced induce VBN cord-319626-f1b3ygg0 212 29 cell cell NN cord-319626-f1b3ygg0 212 30 fusion fusion NN cord-319626-f1b3ygg0 212 31 ( ( -LRB- cord-319626-f1b3ygg0 212 32 Sturman Sturman NNP cord-319626-f1b3ygg0 212 33 and and CC cord-319626-f1b3ygg0 212 34 Holmes Holmes NNP cord-319626-f1b3ygg0 212 35 , , , cord-319626-f1b3ygg0 212 36 1985 1985 CD cord-319626-f1b3ygg0 212 37 ) ) -RRB- cord-319626-f1b3ygg0 212 38 and and CC cord-319626-f1b3ygg0 212 39 we -PRON- PRP cord-319626-f1b3ygg0 212 40 attribute attribute VBP cord-319626-f1b3ygg0 212 41 the the DT cord-319626-f1b3ygg0 212 42 lack lack NN cord-319626-f1b3ygg0 212 43 of of IN cord-319626-f1b3ygg0 212 44 growth growth NN cord-319626-f1b3ygg0 212 45 of of IN cord-319626-f1b3ygg0 212 46 the the DT cord-319626-f1b3ygg0 212 47 syncytia syncytia NN cord-319626-f1b3ygg0 212 48 to to IN cord-319626-f1b3ygg0 212 49 the the DT cord-319626-f1b3ygg0 212 50 failure failure NN cord-319626-f1b3ygg0 212 51 in in IN cord-319626-f1b3ygg0 212 52 the the DT cord-319626-f1b3ygg0 212 53 transport transport NN cord-319626-f1b3ygg0 212 54 of of IN cord-319626-f1b3ygg0 212 55 virions virion NNS cord-319626-f1b3ygg0 212 56 out out IN cord-319626-f1b3ygg0 212 57 of of IN cord-319626-f1b3ygg0 212 58 the the DT cord-319626-f1b3ygg0 212 59 cells cell NNS cord-319626-f1b3ygg0 212 60 . . . cord-319626-f1b3ygg0 213 1 There there EX cord-319626-f1b3ygg0 213 2 may may MD cord-319626-f1b3ygg0 213 3 also also RB cord-319626-f1b3ygg0 213 4 be be VB cord-319626-f1b3ygg0 213 5 , , , cord-319626-f1b3ygg0 213 6 at at IN cord-319626-f1b3ygg0 213 7 31 31 CD cord-319626-f1b3ygg0 213 8 ° ° NN cord-319626-f1b3ygg0 213 9 C c NN cord-319626-f1b3ygg0 213 10 , , , cord-319626-f1b3ygg0 213 11 a a DT cord-319626-f1b3ygg0 213 12 reduction reduction NN cord-319626-f1b3ygg0 213 13 in in IN cord-319626-f1b3ygg0 213 14 the the DT cord-319626-f1b3ygg0 213 15 transport transport NN cord-319626-f1b3ygg0 213 16 to to IN cord-319626-f1b3ygg0 213 17 the the DT cord-319626-f1b3ygg0 213 18 plasma plasma NN cord-319626-f1b3ygg0 213 19 membrane membrane NN cord-319626-f1b3ygg0 213 20 of of IN cord-319626-f1b3ygg0 213 21 E2 E2 NNP cord-319626-f1b3ygg0 213 22 as as IN cord-319626-f1b3ygg0 213 23 a a DT cord-319626-f1b3ygg0 213 24 membrane membrane NN cord-319626-f1b3ygg0 213 25 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 213 26 , , , cord-319626-f1b3ygg0 213 27 independent independent JJ cord-319626-f1b3ygg0 213 28 of of IN cord-319626-f1b3ygg0 213 29 the the DT cord-319626-f1b3ygg0 213 30 transport transport NN cord-319626-f1b3ygg0 213 31 of of IN cord-319626-f1b3ygg0 213 32 virions virion NNS cord-319626-f1b3ygg0 213 33 , , , cord-319626-f1b3ygg0 213 34 but but CC cord-319626-f1b3ygg0 213 35 this this DT cord-319626-f1b3ygg0 213 36 was be VBD cord-319626-f1b3ygg0 213 37 not not RB cord-319626-f1b3ygg0 213 38 investigated investigate VBN cord-319626-f1b3ygg0 213 39 . . . cord-319626-f1b3ygg0 214 1 This this DT cord-319626-f1b3ygg0 214 2 inhibition inhibition NN cord-319626-f1b3ygg0 214 3 of of IN cord-319626-f1b3ygg0 214 4 the the DT cord-319626-f1b3ygg0 214 5 enlargement enlargement NN cord-319626-f1b3ygg0 214 6 of of IN cord-319626-f1b3ygg0 214 7 the the DT cord-319626-f1b3ygg0 214 8 syncytia syncytia NN cord-319626-f1b3ygg0 214 9 was be VBD cord-319626-f1b3ygg0 214 10 reversible reversible JJ cord-319626-f1b3ygg0 214 11 ; ; : cord-319626-f1b3ygg0 214 12 they -PRON- PRP cord-319626-f1b3ygg0 214 13 expanded expand VBD cord-319626-f1b3ygg0 214 14 rapidly rapidly RB cord-319626-f1b3ygg0 214 15 within within IN cord-319626-f1b3ygg0 214 16 2 2 CD cord-319626-f1b3ygg0 214 17 - - SYM cord-319626-f1b3ygg0 214 18 3 3 CD cord-319626-f1b3ygg0 214 19 h h NN cord-319626-f1b3ygg0 214 20 after after IN cord-319626-f1b3ygg0 214 21 a a DT cord-319626-f1b3ygg0 214 22 return return NN cord-319626-f1b3ygg0 214 23 to to IN cord-319626-f1b3ygg0 214 24 37 37 CD cord-319626-f1b3ygg0 214 25 ° ° NN cord-319626-f1b3ygg0 214 26 C c NN cord-319626-f1b3ygg0 214 27 ( ( -LRB- cord-319626-f1b3ygg0 214 28 data datum NNS cord-319626-f1b3ygg0 214 29 not not RB cord-319626-f1b3ygg0 214 30 shown show VBN cord-319626-f1b3ygg0 214 31 ) ) -RRB- cord-319626-f1b3ygg0 214 32 . . . cord-319626-f1b3ygg0 215 1 Budding bud VBG cord-319626-f1b3ygg0 215 2 of of IN cord-319626-f1b3ygg0 215 3 progeny progeny JJ cord-319626-f1b3ygg0 215 4 virions virion NNS cord-319626-f1b3ygg0 215 5 , , , cord-319626-f1b3ygg0 215 6 which which WDT cord-319626-f1b3ygg0 215 7 occurs occur VBZ cord-319626-f1b3ygg0 215 8 at at IN cord-319626-f1b3ygg0 215 9 membranes membrane NNS cord-319626-f1b3ygg0 215 10 with with IN cord-319626-f1b3ygg0 215 11 a a DT cord-319626-f1b3ygg0 215 12 high high JJ cord-319626-f1b3ygg0 215 13 concentration concentration NN cord-319626-f1b3ygg0 215 14 of of IN cord-319626-f1b3ygg0 215 15 the the DT cord-319626-f1b3ygg0 215 16 viral viral JJ cord-319626-f1b3ygg0 215 17 El El NNP cord-319626-f1b3ygg0 215 18 spike spike NN cord-319626-f1b3ygg0 215 19 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 215 20 , , , cord-319626-f1b3ygg0 215 21 was be VBD cord-319626-f1b3ygg0 215 22 first first RB cord-319626-f1b3ygg0 215 23 detected detect VBN cord-319626-f1b3ygg0 215 24 in in IN cord-319626-f1b3ygg0 215 25 thin thin JJ cord-319626-f1b3ygg0 215 26 sections section NNS cord-319626-f1b3ygg0 215 27 under under IN cord-319626-f1b3ygg0 215 28 the the DT cord-319626-f1b3ygg0 215 29 electron electron NN cord-319626-f1b3ygg0 215 30 microscope microscope NN cord-319626-f1b3ygg0 215 31 between between IN cord-319626-f1b3ygg0 215 32 6 6 CD cord-319626-f1b3ygg0 215 33 and and CC cord-319626-f1b3ygg0 215 34 7 7 CD cord-319626-f1b3ygg0 215 35 h h NN cord-319626-f1b3ygg0 215 36 after after IN cord-319626-f1b3ygg0 215 37 infection infection NN cord-319626-f1b3ygg0 215 38 . . . cord-319626-f1b3ygg0 216 1 At at IN cord-319626-f1b3ygg0 216 2 these these DT cord-319626-f1b3ygg0 216 3 early early JJ cord-319626-f1b3ygg0 216 4 times time NNS cord-319626-f1b3ygg0 216 5 , , , cord-319626-f1b3ygg0 216 6 the the DT cord-319626-f1b3ygg0 216 7 first first JJ cord-319626-f1b3ygg0 216 8 and and CC cord-319626-f1b3ygg0 216 9 only only JJ cord-319626-f1b3ygg0 216 10 site site NN cord-319626-f1b3ygg0 216 11 of of IN cord-319626-f1b3ygg0 216 12 budding bud VBG cord-319626-f1b3ygg0 216 13 was be VBD cord-319626-f1b3ygg0 216 14 a a DT cord-319626-f1b3ygg0 216 15 smooth smooth JJ cord-319626-f1b3ygg0 216 16 membraned membraned JJ cord-319626-f1b3ygg0 216 17 compartment compartment NN cord-319626-f1b3ygg0 216 18 with with IN cord-319626-f1b3ygg0 216 19 a a DT cord-319626-f1b3ygg0 216 20 complex complex JJ cord-319626-f1b3ygg0 216 21 and and CC cord-319626-f1b3ygg0 216 22 irregular irregular JJ cord-319626-f1b3ygg0 216 23 vesiculotubular vesiculotubular JJ cord-319626-f1b3ygg0 216 24 morphology morphology NN cord-319626-f1b3ygg0 216 25 ( ( -LRB- cord-319626-f1b3ygg0 216 26 Fig Fig NNP cord-319626-f1b3ygg0 216 27 . . . cord-319626-f1b3ygg0 216 28 8) 8) NNP cord-319626-f1b3ygg0 216 29 . . . cord-319626-f1b3ygg0 217 1 This this DT cord-319626-f1b3ygg0 217 2 compartment compartment NN cord-319626-f1b3ygg0 217 3 , , , cord-319626-f1b3ygg0 217 4 which which WDT cord-319626-f1b3ygg0 217 5 we -PRON- PRP cord-319626-f1b3ygg0 217 6 refer refer VBP cord-319626-f1b3ygg0 217 7 to to IN cord-319626-f1b3ygg0 217 8 here here RB cord-319626-f1b3ygg0 217 9 as as IN cord-319626-f1b3ygg0 217 10 the the DT cord-319626-f1b3ygg0 217 11 budding bud VBG cord-319626-f1b3ygg0 217 12 compartment compartment NN cord-319626-f1b3ygg0 217 13 , , , cord-319626-f1b3ygg0 217 14 was be VBD cord-319626-f1b3ygg0 217 15 always always RB cord-319626-f1b3ygg0 217 16 closely closely RB cord-319626-f1b3ygg0 217 17 associated associate VBN cord-319626-f1b3ygg0 217 18 with with IN cord-319626-f1b3ygg0 217 19 cisternae cisternae NN cord-319626-f1b3ygg0 217 20 of of IN cord-319626-f1b3ygg0 217 21 the the DT cord-319626-f1b3ygg0 217 22 rough rough JJ cord-319626-f1b3ygg0 217 23 ER er NN cord-319626-f1b3ygg0 217 24 , , , cord-319626-f1b3ygg0 217 25 including include VBG cord-319626-f1b3ygg0 217 26 transitional transitional JJ cord-319626-f1b3ygg0 217 27 elements element NNS cord-319626-f1b3ygg0 217 28 . . . cord-319626-f1b3ygg0 218 1 Usually usually RB cord-319626-f1b3ygg0 218 2 , , , cord-319626-f1b3ygg0 218 3 but but CC cord-319626-f1b3ygg0 218 4 not not RB cord-319626-f1b3ygg0 218 5 always always RB cord-319626-f1b3ygg0 218 6 , , , cord-319626-f1b3ygg0 218 7 the the DT cord-319626-f1b3ygg0 218 8 budding bud VBG cord-319626-f1b3ygg0 218 9 compartment compartment NN cord-319626-f1b3ygg0 218 10 was be VBD cord-319626-f1b3ygg0 218 11 also also RB cord-319626-f1b3ygg0 218 12 juxtaposed juxtapose VBN cord-319626-f1b3ygg0 218 13 to to IN cord-319626-f1b3ygg0 218 14 the the DT cord-319626-f1b3ygg0 218 15 cis cis NNP cord-319626-f1b3ygg0 218 16 side side NN cord-319626-f1b3ygg0 218 17 of of IN cord-319626-f1b3ygg0 218 18 the the DT cord-319626-f1b3ygg0 218 19 Golgi Golgi NNP cord-319626-f1b3ygg0 218 20 stack stack NN cord-319626-f1b3ygg0 218 21 ( ( -LRB- cord-319626-f1b3ygg0 218 22 see see VB cord-319626-f1b3ygg0 218 23 Fig Fig NNP cord-319626-f1b3ygg0 218 24 . . . cord-319626-f1b3ygg0 218 25 11 11 CD cord-319626-f1b3ygg0 218 26 ) ) -RRB- cord-319626-f1b3ygg0 218 27 . . . cord-319626-f1b3ygg0 219 1 Since since IN cord-319626-f1b3ygg0 219 2 the the DT cord-319626-f1b3ygg0 219 3 membrane membrane NN cord-319626-f1b3ygg0 219 4 of of IN cord-319626-f1b3ygg0 219 5 the the DT cord-319626-f1b3ygg0 219 6 budding bud VBG cord-319626-f1b3ygg0 219 7 compartment compartment NN cord-319626-f1b3ygg0 219 8 is be VBZ cord-319626-f1b3ygg0 219 9 smooth smooth JJ cord-319626-f1b3ygg0 219 10 it -PRON- PRP cord-319626-f1b3ygg0 219 11 can can MD cord-319626-f1b3ygg0 219 12 not not RB cord-319626-f1b3ygg0 219 13 be be VB cord-319626-f1b3ygg0 219 14 the the DT cord-319626-f1b3ygg0 219 15 site site NN cord-319626-f1b3ygg0 219 16 of of IN cord-319626-f1b3ygg0 219 17 synthesis synthesis NN cord-319626-f1b3ygg0 219 18 of of IN cord-319626-f1b3ygg0 219 19 E1 E1 NNP cord-319626-f1b3ygg0 219 20 or or CC cord-319626-f1b3ygg0 219 21 its -PRON- PRP$ cord-319626-f1b3ygg0 219 22 insertion insertion NN cord-319626-f1b3ygg0 219 23 into into IN cord-319626-f1b3ygg0 219 24 membranes membrane NNS cord-319626-f1b3ygg0 219 25 . . . cord-319626-f1b3ygg0 220 1 Furthermore furthermore RB cord-319626-f1b3ygg0 220 2 , , , cord-319626-f1b3ygg0 220 3 we -PRON- PRP cord-319626-f1b3ygg0 220 4 have have VBP cord-319626-f1b3ygg0 220 5 reported report VBN cord-319626-f1b3ygg0 220 6 previously previously RB cord-319626-f1b3ygg0 220 7 that that IN cord-319626-f1b3ygg0 220 8 the the DT cord-319626-f1b3ygg0 220 9 budding bud VBG cord-319626-f1b3ygg0 220 10 compartment compartment NN cord-319626-f1b3ygg0 220 11 does do VBZ cord-319626-f1b3ygg0 220 12 not not RB cord-319626-f1b3ygg0 220 13 contain contain VB cord-319626-f1b3ygg0 220 14 a a DT cord-319626-f1b3ygg0 220 15 cytochemical cytochemical JJ cord-319626-f1b3ygg0 220 16 marker marker NN cord-319626-f1b3ygg0 220 17 of of IN cord-319626-f1b3ygg0 220 18 the the DT cord-319626-f1b3ygg0 220 19 rough rough JJ cord-319626-f1b3ygg0 220 20 ER er NN cord-319626-f1b3ygg0 221 1 ( ( -LRB- cord-319626-f1b3ygg0 221 2 Tooze Tooze NNP cord-319626-f1b3ygg0 221 3 et et NNP cord-319626-f1b3ygg0 221 4 al al NNP cord-319626-f1b3ygg0 221 5 . . NNP cord-319626-f1b3ygg0 221 6 , , , cord-319626-f1b3ygg0 221 7 1984 1984 CD cord-319626-f1b3ygg0 221 8 ) ) -RRB- cord-319626-f1b3ygg0 221 9 . . . cord-319626-f1b3ygg0 222 1 We -PRON- PRP cord-319626-f1b3ygg0 222 2 conclude conclude VBP cord-319626-f1b3ygg0 222 3 that that IN cord-319626-f1b3ygg0 222 4 the the DT cord-319626-f1b3ygg0 222 5 initial initial JJ cord-319626-f1b3ygg0 222 6 site site NN cord-319626-f1b3ygg0 222 7 of of IN cord-319626-f1b3ygg0 222 8 accumulation accumulation NN cord-319626-f1b3ygg0 222 9 of of IN cord-319626-f1b3ygg0 222 10 E1 e1 NN cord-319626-f1b3ygg0 222 11 to to IN cord-319626-f1b3ygg0 222 12 levels level NNS cord-319626-f1b3ygg0 222 13 that that WDT cord-319626-f1b3ygg0 222 14 support support VBP cord-319626-f1b3ygg0 222 15 budding budding NN cord-319626-f1b3ygg0 222 16 is be VBZ cord-319626-f1b3ygg0 222 17 a a DT cord-319626-f1b3ygg0 222 18 distinct distinct JJ cord-319626-f1b3ygg0 222 19 smooth- smooth- NN cord-319626-f1b3ygg0 222 20 membraned membrane VBN cord-319626-f1b3ygg0 222 21 compartment compartment NN cord-319626-f1b3ygg0 222 22 usually usually RB cord-319626-f1b3ygg0 222 23 found find VBN cord-319626-f1b3ygg0 222 24 between between IN cord-319626-f1b3ygg0 222 25 transitional transitional JJ cord-319626-f1b3ygg0 222 26 elements element NNS cord-319626-f1b3ygg0 222 27 of of IN cord-319626-f1b3ygg0 222 28 the the DT cord-319626-f1b3ygg0 222 29 rough rough JJ cord-319626-f1b3ygg0 222 30 ER er NN cord-319626-f1b3ygg0 222 31 and and CC cord-319626-f1b3ygg0 222 32 usually usually RB cord-319626-f1b3ygg0 222 33 lies lie VBZ cord-319626-f1b3ygg0 222 34 between between IN cord-319626-f1b3ygg0 222 35 them -PRON- PRP cord-319626-f1b3ygg0 222 36 and and CC cord-319626-f1b3ygg0 222 37 the the DT cord-319626-f1b3ygg0 222 38 Golgi Golgi NNP cord-319626-f1b3ygg0 222 39 complex complex NN cord-319626-f1b3ygg0 222 40 . . . cord-319626-f1b3ygg0 223 1 Examination examination NN cord-319626-f1b3ygg0 223 2 of of IN cord-319626-f1b3ygg0 223 3 single single JJ cord-319626-f1b3ygg0 223 4 and and CC cord-319626-f1b3ygg0 223 5 serial serial JJ cord-319626-f1b3ygg0 223 6 sections section NNS cord-319626-f1b3ygg0 223 7 showed show VBD cord-319626-f1b3ygg0 223 8 that that IN cord-319626-f1b3ygg0 223 9 this this DT cord-319626-f1b3ygg0 223 10 distinct distinct JJ cord-319626-f1b3ygg0 223 11 compartment compartment NN cord-319626-f1b3ygg0 223 12 also also RB cord-319626-f1b3ygg0 223 13 exists exist VBZ cord-319626-f1b3ygg0 223 14 in in IN cord-319626-f1b3ygg0 223 15 uninfected uninfected JJ cord-319626-f1b3ygg0 223 16 cells cell NNS cord-319626-f1b3ygg0 223 17 ( ( -LRB- cord-319626-f1b3ygg0 223 18 Fig.9 Fig.9 NNS cord-319626-f1b3ygg0 223 19 ) ) -RRB- cord-319626-f1b3ygg0 223 20 , , , cord-319626-f1b3ygg0 223 21 where where WRB cord-319626-f1b3ygg0 223 22 it -PRON- PRP cord-319626-f1b3ygg0 223 23 is be VBZ cord-319626-f1b3ygg0 223 24 also also RB cord-319626-f1b3ygg0 223 25 invariably invariably RB cord-319626-f1b3ygg0 223 26 associated associate VBN cord-319626-f1b3ygg0 223 27 with with IN cord-319626-f1b3ygg0 223 28 transitional transitional JJ cord-319626-f1b3ygg0 223 29 elements element NNS cord-319626-f1b3ygg0 223 30 of of IN cord-319626-f1b3ygg0 223 31 the the DT cord-319626-f1b3ygg0 223 32 rough rough JJ cord-319626-f1b3ygg0 223 33 ER er NN cord-319626-f1b3ygg0 223 34 and and CC cord-319626-f1b3ygg0 223 35 Golgi Golgi NNP cord-319626-f1b3ygg0 223 36 stacks stack NNS cord-319626-f1b3ygg0 223 37 . . . cord-319626-f1b3ygg0 224 1 Comparison comparison NN cord-319626-f1b3ygg0 224 2 of of IN cord-319626-f1b3ygg0 224 3 Figs Figs NNPS cord-319626-f1b3ygg0 224 4 . . . cord-319626-f1b3ygg0 225 1 8 8 CD cord-319626-f1b3ygg0 225 2 and and CC cord-319626-f1b3ygg0 225 3 9 9 CD cord-319626-f1b3ygg0 225 4 shows show VBZ cord-319626-f1b3ygg0 225 5 that that IN cord-319626-f1b3ygg0 225 6 the the DT cord-319626-f1b3ygg0 225 7 budding bud VBG cord-319626-f1b3ygg0 225 8 compartment compartment NN cord-319626-f1b3ygg0 225 9 is be VBZ cord-319626-f1b3ygg0 225 10 more more RBR cord-319626-f1b3ygg0 225 11 dilated dilated JJ cord-319626-f1b3ygg0 225 12 in in IN cord-319626-f1b3ygg0 225 13 infected infect VBN cord-319626-f1b3ygg0 225 14 than than IN cord-319626-f1b3ygg0 225 15 in in IN cord-319626-f1b3ygg0 225 16 uninfected uninfected JJ cord-319626-f1b3ygg0 225 17 cells cell NNS cord-319626-f1b3ygg0 225 18 . . . cord-319626-f1b3ygg0 226 1 In in IN cord-319626-f1b3ygg0 226 2 part part NN cord-319626-f1b3ygg0 226 3 , , , cord-319626-f1b3ygg0 226 4 no no RB cord-319626-f1b3ygg0 226 5 doubt doubt RB cord-319626-f1b3ygg0 226 6 , , , cord-319626-f1b3ygg0 226 7 this this DT cord-319626-f1b3ygg0 226 8 is be VBZ cord-319626-f1b3ygg0 226 9 a a DT cord-319626-f1b3ygg0 226 10 result result NN cord-319626-f1b3ygg0 226 11 of of IN cord-319626-f1b3ygg0 226 12 the the DT cord-319626-f1b3ygg0 226 13 budding budding NN cord-319626-f1b3ygg0 226 14 of of IN cord-319626-f1b3ygg0 226 15 virions virion NNS cord-319626-f1b3ygg0 226 16 , , , cord-319626-f1b3ygg0 226 17 each each DT cord-319626-f1b3ygg0 226 18 ~1,000/~ ~1,000/~ NN cord-319626-f1b3ygg0 227 1 in in IN cord-319626-f1b3ygg0 227 2 diam diam NNP cord-319626-f1b3ygg0 227 3 , , , cord-319626-f1b3ygg0 227 4 into into IN cord-319626-f1b3ygg0 227 5 this this DT cord-319626-f1b3ygg0 227 6 compartment compartment NN cord-319626-f1b3ygg0 227 7 in in IN cord-319626-f1b3ygg0 227 8 the the DT cord-319626-f1b3ygg0 227 9 infected infected JJ cord-319626-f1b3ygg0 227 10 cells cell NNS cord-319626-f1b3ygg0 227 11 . . . cord-319626-f1b3ygg0 228 1 Although although IN cord-319626-f1b3ygg0 228 2 we -PRON- PRP cord-319626-f1b3ygg0 228 3 have have VBP cord-319626-f1b3ygg0 228 4 not not RB cord-319626-f1b3ygg0 228 5 made make VBN cord-319626-f1b3ygg0 228 6 extensive extensive JJ cord-319626-f1b3ygg0 228 7 morphometric morphometric JJ cord-319626-f1b3ygg0 228 8 analyses analysis NNS cord-319626-f1b3ygg0 228 9 of of IN cord-319626-f1b3ygg0 228 10 the the DT cord-319626-f1b3ygg0 228 11 budding bud VBG cord-319626-f1b3ygg0 228 12 compartment compartment NN cord-319626-f1b3ygg0 228 13 , , , cord-319626-f1b3ygg0 228 14 it -PRON- PRP cord-319626-f1b3ygg0 228 15 is be VBZ cord-319626-f1b3ygg0 228 16 clear clear JJ cord-319626-f1b3ygg0 228 17 from from IN cord-319626-f1b3ygg0 228 18 examination examination NN cord-319626-f1b3ygg0 228 19 of of IN cord-319626-f1b3ygg0 228 20 single single JJ cord-319626-f1b3ygg0 228 21 and and CC cord-319626-f1b3ygg0 228 22 serial serial JJ cord-319626-f1b3ygg0 228 23 sections section NNS cord-319626-f1b3ygg0 228 24 that that WDT cord-319626-f1b3ygg0 228 25 its -PRON- PRP$ cord-319626-f1b3ygg0 228 26 volume volume NN cord-319626-f1b3ygg0 228 27 and and CC cord-319626-f1b3ygg0 228 28 surface surface NN cord-319626-f1b3ygg0 228 29 area area NN cord-319626-f1b3ygg0 228 30 are be VBP cord-319626-f1b3ygg0 228 31 smaller small JJR cord-319626-f1b3ygg0 228 32 than than IN cord-319626-f1b3ygg0 228 33 those those DT cord-319626-f1b3ygg0 228 34 of of IN cord-319626-f1b3ygg0 228 35 either either CC cord-319626-f1b3ygg0 228 36 the the DT cord-319626-f1b3ygg0 228 37 Golgi Golgi NNP cord-319626-f1b3ygg0 228 38 complex complex NN cord-319626-f1b3ygg0 228 39 or or CC cord-319626-f1b3ygg0 228 40 the the DT cord-319626-f1b3ygg0 228 41 rough rough JJ cord-319626-f1b3ygg0 228 42 ER er NN cord-319626-f1b3ygg0 228 43 . . . cord-319626-f1b3ygg0 229 1 Beginning begin VBG cord-319626-f1b3ygg0 229 2 between between IN cord-319626-f1b3ygg0 229 3 8 8 CD cord-319626-f1b3ygg0 229 4 and and CC cord-319626-f1b3ygg0 229 5 9 9 CD cord-319626-f1b3ygg0 229 6 h h NN cord-319626-f1b3ygg0 229 7 after after IN cord-319626-f1b3ygg0 229 8 infection infection NN cord-319626-f1b3ygg0 229 9 at at IN cord-319626-f1b3ygg0 229 10 37 37 CD cord-319626-f1b3ygg0 229 11 ° ° NN cord-319626-f1b3ygg0 229 12 C c NN cord-319626-f1b3ygg0 229 13 , , , cord-319626-f1b3ygg0 229 14 the the DT cord-319626-f1b3ygg0 229 15 rough rough JJ cord-319626-f1b3ygg0 229 16 ER er NN cord-319626-f1b3ygg0 229 17 becomes become VBZ cord-319626-f1b3ygg0 229 18 a a DT cord-319626-f1b3ygg0 229 19 site site NN cord-319626-f1b3ygg0 229 20 of of IN cord-319626-f1b3ygg0 229 21 budding budding NN cord-319626-f1b3ygg0 229 22 of of IN cord-319626-f1b3ygg0 229 23 virions virion NNS cord-319626-f1b3ygg0 229 24 and and CC cord-319626-f1b3ygg0 229 25 progressively progressively RB cord-319626-f1b3ygg0 229 26 more more JJR cord-319626-f1b3ygg0 229 27 viruses virus NNS cord-319626-f1b3ygg0 229 28 bud bud VBP cord-319626-f1b3ygg0 229 29 and and CC cord-319626-f1b3ygg0 229 30 accumulate accumulate VBP cord-319626-f1b3ygg0 229 31 in in IN cord-319626-f1b3ygg0 229 32 this this DT cord-319626-f1b3ygg0 229 33 compartment compartment NN cord-319626-f1b3ygg0 229 34 ( ( -LRB- cord-319626-f1b3ygg0 229 35 Tooze Tooze NNP cord-319626-f1b3ygg0 229 36 et et NNP cord-319626-f1b3ygg0 229 37 al al NNP cord-319626-f1b3ygg0 229 38 . . . cord-319626-f1b3ygg0 230 1 , , , cord-319626-f1b3ygg0 230 2 1984 1984 CD cord-319626-f1b3ygg0 231 1 ( ( -LRB- cord-319626-f1b3ygg0 231 2 Tooze Tooze NNP cord-319626-f1b3ygg0 231 3 et et NNP cord-319626-f1b3ygg0 231 4 al al NNP cord-319626-f1b3ygg0 231 5 . . NNP cord-319626-f1b3ygg0 231 6 , , , cord-319626-f1b3ygg0 231 7 , , , cord-319626-f1b3ygg0 231 8 1985 1985 CD cord-319626-f1b3ygg0 231 9 . . . cord-319626-f1b3ygg0 232 1 Virions virion NNS cord-319626-f1b3ygg0 232 2 can can MD cord-319626-f1b3ygg0 232 3 also also RB cord-319626-f1b3ygg0 232 4 be be VB cord-319626-f1b3ygg0 232 5 seen see VBN cord-319626-f1b3ygg0 232 6 increasingly increasingly RB cord-319626-f1b3ygg0 232 7 in in IN cord-319626-f1b3ygg0 232 8 the the DT cord-319626-f1b3ygg0 232 9 rims rim NNS cord-319626-f1b3ygg0 232 10 of of IN cord-319626-f1b3ygg0 232 11 Golgi Golgi NNP cord-319626-f1b3ygg0 232 12 cisternae cisternae NNP cord-319626-f1b3ygg0 232 13 and and CC cord-319626-f1b3ygg0 232 14 in in IN cord-319626-f1b3ygg0 232 15 post post JJ cord-319626-f1b3ygg0 232 16 - - JJ cord-319626-f1b3ygg0 232 17 Golgi golgi JJ cord-319626-f1b3ygg0 232 18 vesicles vesicle NNS cord-319626-f1b3ygg0 232 19 transporting transport VBG cord-319626-f1b3ygg0 232 20 the the DT cord-319626-f1b3ygg0 232 21 virus virus NN cord-319626-f1b3ygg0 232 22 to to IN cord-319626-f1b3ygg0 232 23 the the DT cord-319626-f1b3ygg0 232 24 cell cell NN cord-319626-f1b3ygg0 232 25 surface surface NN cord-319626-f1b3ygg0 232 26 . . . cord-319626-f1b3ygg0 233 1 The the DT cord-319626-f1b3ygg0 233 2 Golgi Golgi NNP cord-319626-f1b3ygg0 233 3 cisternae cisternae NN cord-319626-f1b3ygg0 233 4 become become VBP cord-319626-f1b3ygg0 233 5 distended distended JJ cord-319626-f1b3ygg0 233 6 and and CC cord-319626-f1b3ygg0 233 7 eventually eventually RB cord-319626-f1b3ygg0 233 8 vesiculate vesiculate VBP cord-319626-f1b3ygg0 233 9 ; ; : cord-319626-f1b3ygg0 233 10 by by IN cord-319626-f1b3ygg0 233 11 12 12 CD cord-319626-f1b3ygg0 233 12 h h NN cord-319626-f1b3ygg0 233 13 after after IN cord-319626-f1b3ygg0 233 14 infection infection NN cord-319626-f1b3ygg0 233 15 cell cell NN cord-319626-f1b3ygg0 233 16 lysis lysis NN cord-319626-f1b3ygg0 233 17 starts start VBZ cord-319626-f1b3ygg0 233 18 ( ( -LRB- cord-319626-f1b3ygg0 234 1 Tooze Tooze NNP cord-319626-f1b3ygg0 234 2 et et NNP cord-319626-f1b3ygg0 234 3 al al NNP cord-319626-f1b3ygg0 234 4 . . NNP cord-319626-f1b3ygg0 234 5 , , , cord-319626-f1b3ygg0 234 6 1984 1984 CD cord-319626-f1b3ygg0 234 7 ; ; : cord-319626-f1b3ygg0 234 8 and and CC cord-319626-f1b3ygg0 234 9 unpublished unpublished JJ cord-319626-f1b3ygg0 234 10 observations observation NNS cord-319626-f1b3ygg0 234 11 ) ) -RRB- cord-319626-f1b3ygg0 234 12 . . . cord-319626-f1b3ygg0 235 1 By by IN cord-319626-f1b3ygg0 235 2 contrast contrast NN cord-319626-f1b3ygg0 235 3 , , , cord-319626-f1b3ygg0 235 4 when when WRB cord-319626-f1b3ygg0 235 5 cultures culture NNS cord-319626-f1b3ygg0 235 6 are be VBP cord-319626-f1b3ygg0 235 7 shifted shift VBN cord-319626-f1b3ygg0 235 8 at at IN cord-319626-f1b3ygg0 235 9 5 5 CD cord-319626-f1b3ygg0 235 10 - - SYM cord-319626-f1b3ygg0 235 11 7 7 CD cord-319626-f1b3ygg0 235 12 h h NN cord-319626-f1b3ygg0 235 13 after after IN cord-319626-f1b3ygg0 235 14 infection infection NN cord-319626-f1b3ygg0 235 15 from from IN cord-319626-f1b3ygg0 235 16 37 37 CD cord-319626-f1b3ygg0 235 17 to to TO cord-319626-f1b3ygg0 235 18 31 31 CD cord-319626-f1b3ygg0 235 19 ° ° NN cord-319626-f1b3ygg0 235 20 C c NN cord-319626-f1b3ygg0 235 21 , , , cord-319626-f1b3ygg0 235 22 the the DT cord-319626-f1b3ygg0 235 23 infected infected JJ cord-319626-f1b3ygg0 235 24 cells cell NNS cord-319626-f1b3ygg0 235 25 survive survive VBP cord-319626-f1b3ygg0 235 26 for for IN cord-319626-f1b3ygg0 235 27 at at IN cord-319626-f1b3ygg0 235 28 least least JJS cord-319626-f1b3ygg0 235 29 a a DT cord-319626-f1b3ygg0 235 30 further further JJ cord-319626-f1b3ygg0 235 31 18 18 CD cord-319626-f1b3ygg0 235 32 h. h. NN cord-319626-f1b3ygg0 236 1 At at IN cord-319626-f1b3ygg0 236 2 24 24 CD cord-319626-f1b3ygg0 236 3 h h NN cord-319626-f1b3ygg0 236 4 after after IN cord-319626-f1b3ygg0 236 5 infection infection NN cord-319626-f1b3ygg0 236 6 , , , cord-319626-f1b3ygg0 236 7 their -PRON- PRP$ cord-319626-f1b3ygg0 236 8 Golgi Golgi NNP cord-319626-f1b3ygg0 236 9 stacks stack NNS cord-319626-f1b3ygg0 236 10 have have VBP cord-319626-f1b3ygg0 236 11 retained retain VBN cord-319626-f1b3ygg0 236 12 their -PRON- PRP$ cord-319626-f1b3ygg0 236 13 morphological morphological JJ cord-319626-f1b3ygg0 236 14 integrity integrity NN cord-319626-f1b3ygg0 236 15 . . . cord-319626-f1b3ygg0 237 1 Few few JJ cord-319626-f1b3ygg0 237 2 virions virion NNS cord-319626-f1b3ygg0 237 3 are be VBP cord-319626-f1b3ygg0 237 4 seen see VBN cord-319626-f1b3ygg0 237 5 within within IN cord-319626-f1b3ygg0 237 6 the the DT cord-319626-f1b3ygg0 237 7 Golgi Golgi NNP cord-319626-f1b3ygg0 237 8 cisternae cisternae NNP cord-319626-f1b3ygg0 237 9 or or CC cord-319626-f1b3ygg0 237 10 in in IN cord-319626-f1b3ygg0 237 11 post post JJ cord-319626-f1b3ygg0 237 12 - - JJ cord-319626-f1b3ygg0 237 13 Golgi golgi JJ cord-319626-f1b3ygg0 237 14 transport transport NN cord-319626-f1b3ygg0 237 15 vesicles vesicle NNS cord-319626-f1b3ygg0 237 16 ( ( -LRB- cord-319626-f1b3ygg0 237 17 Fig Fig NNP cord-319626-f1b3ygg0 237 18 . . . cord-319626-f1b3ygg0 237 19 10 10 CD cord-319626-f1b3ygg0 237 20 ) ) -RRB- cord-319626-f1b3ygg0 237 21 , , , cord-319626-f1b3ygg0 237 22 and and CC cord-319626-f1b3ygg0 237 23 very very RB cord-319626-f1b3ygg0 237 24 few few JJ cord-319626-f1b3ygg0 237 25 are be VBP cord-319626-f1b3ygg0 237 26 seen see VBN cord-319626-f1b3ygg0 237 27 outside outside IN cord-319626-f1b3ygg0 237 28 the the DT cord-319626-f1b3ygg0 237 29 cells cell NNS cord-319626-f1b3ygg0 237 30 associated associate VBN cord-319626-f1b3ygg0 237 31 with with IN cord-319626-f1b3ygg0 237 32 the the DT cord-319626-f1b3ygg0 237 33 plasma plasma NN cord-319626-f1b3ygg0 237 34 membrane membrane NN cord-319626-f1b3ygg0 237 35 . . . cord-319626-f1b3ygg0 238 1 On on IN cord-319626-f1b3ygg0 238 2 the the DT cord-319626-f1b3ygg0 238 3 other other JJ cord-319626-f1b3ygg0 238 4 hand hand NN cord-319626-f1b3ygg0 238 5 , , , cord-319626-f1b3ygg0 238 6 the the DT cord-319626-f1b3ygg0 238 7 rough rough JJ cord-319626-f1b3ygg0 238 8 ER ER NNP cord-319626-f1b3ygg0 238 9 contains contain VBZ cord-319626-f1b3ygg0 238 10 massive massive JJ cord-319626-f1b3ygg0 238 11 accumulations accumulation NNS cord-319626-f1b3ygg0 238 12 of of IN cord-319626-f1b3ygg0 238 13 virions virion NNS cord-319626-f1b3ygg0 238 14 ( ( -LRB- cord-319626-f1b3ygg0 238 15 Fig Fig NNP cord-319626-f1b3ygg0 238 16 . . . cord-319626-f1b3ygg0 238 17 10 10 CD cord-319626-f1b3ygg0 238 18 ) ) -RRB- cord-319626-f1b3ygg0 238 19 . . . cord-319626-f1b3ygg0 239 1 These these DT cord-319626-f1b3ygg0 239 2 morphological morphological JJ cord-319626-f1b3ygg0 239 3 observations observation NNS cord-319626-f1b3ygg0 239 4 indicate indicate VBP cord-319626-f1b3ygg0 239 5 that that IN cord-319626-f1b3ygg0 239 6 there there EX cord-319626-f1b3ygg0 239 7 is be VBZ cord-319626-f1b3ygg0 239 8 little little JJ cord-319626-f1b3ygg0 239 9 trans trans JJ cord-319626-f1b3ygg0 239 10 - - JJ cord-319626-f1b3ygg0 239 11 port port NN cord-319626-f1b3ygg0 239 12 of of IN cord-319626-f1b3ygg0 239 13 virions virion NNS cord-319626-f1b3ygg0 239 14 into into IN cord-319626-f1b3ygg0 239 15 the the DT cord-319626-f1b3ygg0 239 16 Golgi Golgi NNP cord-319626-f1b3ygg0 239 17 stacks stack NNS cord-319626-f1b3ygg0 239 18 and and CC cord-319626-f1b3ygg0 239 19 beyond beyond IN cord-319626-f1b3ygg0 239 20 at at IN cord-319626-f1b3ygg0 239 21 31 31 CD cord-319626-f1b3ygg0 239 22 ° ° NN cord-319626-f1b3ygg0 239 23 C c NN cord-319626-f1b3ygg0 239 24 . . . cord-319626-f1b3ygg0 240 1 We -PRON- PRP cord-319626-f1b3ygg0 240 2 believe believe VBP cord-319626-f1b3ygg0 240 3 that that IN cord-319626-f1b3ygg0 240 4 it -PRON- PRP cord-319626-f1b3ygg0 240 5 is be VBZ cord-319626-f1b3ygg0 240 6 this this DT cord-319626-f1b3ygg0 240 7 block block NN cord-319626-f1b3ygg0 240 8 to to IN cord-319626-f1b3ygg0 240 9 virion virion NN cord-319626-f1b3ygg0 240 10 transport transport NN cord-319626-f1b3ygg0 240 11 that that WDT cord-319626-f1b3ygg0 240 12 prevents prevent VBZ cord-319626-f1b3ygg0 240 13 the the DT cord-319626-f1b3ygg0 240 14 disruption disruption NN cord-319626-f1b3ygg0 240 15 of of IN cord-319626-f1b3ygg0 240 16 the the DT cord-319626-f1b3ygg0 240 17 Golgi Golgi NNP cord-319626-f1b3ygg0 240 18 stacks stack NNS cord-319626-f1b3ygg0 240 19 , , , cord-319626-f1b3ygg0 240 20 which which WDT cord-319626-f1b3ygg0 240 21 is be VBZ cord-319626-f1b3ygg0 240 22 one one CD cord-319626-f1b3ygg0 240 23 of of IN cord-319626-f1b3ygg0 240 24 the the DT cord-319626-f1b3ygg0 240 25 cytopathic cytopathic JJ cord-319626-f1b3ygg0 240 26 effects effect NNS cord-319626-f1b3ygg0 240 27 of of IN cord-319626-f1b3ygg0 240 28 MHV MHV NNP cord-319626-f1b3ygg0 240 29 - - HYPH cord-319626-f1b3ygg0 240 30 A59 A59 NNP cord-319626-f1b3ygg0 240 31 infection infection NN cord-319626-f1b3ygg0 240 32 at at IN cord-319626-f1b3ygg0 240 33 37 37 CD cord-319626-f1b3ygg0 240 34 ° ° NN cord-319626-f1b3ygg0 240 35 C c NN cord-319626-f1b3ygg0 240 36 and and CC cord-319626-f1b3ygg0 240 37 is be VBZ cord-319626-f1b3ygg0 240 38 also also RB cord-319626-f1b3ygg0 240 39 observed observe VBN cord-319626-f1b3ygg0 240 40 in in IN cord-319626-f1b3ygg0 240 41 cells cell NNS cord-319626-f1b3ygg0 240 42 infected infect VBN cord-319626-f1b3ygg0 240 43 at at IN cord-319626-f1b3ygg0 240 44 37 37 CD cord-319626-f1b3ygg0 240 45 ° ° NN cord-319626-f1b3ygg0 240 46 C c NN cord-319626-f1b3ygg0 240 47 with with IN cord-319626-f1b3ygg0 240 48 Uukuniemi Uukuniemi NNP cord-319626-f1b3ygg0 240 49 virus virus NN cord-319626-f1b3ygg0 240 50 , , , cord-319626-f1b3ygg0 240 51 a a DT cord-319626-f1b3ygg0 240 52 bunyavirus bunyavirus NN cord-319626-f1b3ygg0 240 53 that that WDT cord-319626-f1b3ygg0 240 54 buds bud NNS cord-319626-f1b3ygg0 240 55 into into IN cord-319626-f1b3ygg0 240 56 intracellular intracellular JJ cord-319626-f1b3ygg0 240 57 membranes membrane NNS cord-319626-f1b3ygg0 240 58 ( ( -LRB- cord-319626-f1b3ygg0 240 59 Gahmberg Gahmberg NNP cord-319626-f1b3ygg0 240 60 et et FW cord-319626-f1b3ygg0 240 61 al al NNP cord-319626-f1b3ygg0 240 62 . . NNP cord-319626-f1b3ygg0 240 63 , , , cord-319626-f1b3ygg0 240 64 1986 1986 CD cord-319626-f1b3ygg0 240 65 ) ) -RRB- cord-319626-f1b3ygg0 240 66 . . . cord-319626-f1b3ygg0 241 1 Using use VBG cord-319626-f1b3ygg0 241 2 an an DT cord-319626-f1b3ygg0 241 3 immunoperoxidase immunoperoxidase NN cord-319626-f1b3ygg0 241 4 labeling labeling NN cord-319626-f1b3ygg0 241 5 technique technique NN cord-319626-f1b3ygg0 241 6 and and CC cord-319626-f1b3ygg0 241 7 a a DT cord-319626-f1b3ygg0 241 8 mAb mab NN cord-319626-f1b3ygg0 241 9 against against IN cord-319626-f1b3ygg0 241 10 an an DT cord-319626-f1b3ygg0 241 11 amino amino NN cord-319626-f1b3ygg0 241 12 - - HYPH cord-319626-f1b3ygg0 241 13 terminal terminal NN cord-319626-f1b3ygg0 241 14 epitope epitope NN cord-319626-f1b3ygg0 241 15 of of IN cord-319626-f1b3ygg0 241 16 E1 E1 NNP cord-319626-f1b3ygg0 241 17 ( ( -LRB- cord-319626-f1b3ygg0 241 18 Tooze Tooze NNP cord-319626-f1b3ygg0 241 19 and and CC cord-319626-f1b3ygg0 241 20 Stanley Stanley NNP cord-319626-f1b3ygg0 241 21 , , , cord-319626-f1b3ygg0 241 22 1986 1986 CD cord-319626-f1b3ygg0 241 23 ) ) -RRB- cord-319626-f1b3ygg0 241 24 , , , cord-319626-f1b3ygg0 241 25 we -PRON- PRP cord-319626-f1b3ygg0 241 26 determined determine VBD cord-319626-f1b3ygg0 241 27 at at IN cord-319626-f1b3ygg0 241 28 the the DT cord-319626-f1b3ygg0 241 29 level level NN cord-319626-f1b3ygg0 241 30 of of IN cord-319626-f1b3ygg0 241 31 resolution resolution NN cord-319626-f1b3ygg0 241 32 of of IN cord-319626-f1b3ygg0 241 33 the the DT cord-319626-f1b3ygg0 241 34 electron electron NN cord-319626-f1b3ygg0 241 35 microscope microscope NN cord-319626-f1b3ygg0 241 36 which which WDT cord-319626-f1b3ygg0 241 37 membranes membrane NNS cord-319626-f1b3ygg0 241 38 contained contain VBD cord-319626-f1b3ygg0 241 39 E1 E1 NNP cord-319626-f1b3ygg0 241 40 at at IN cord-319626-f1b3ygg0 241 41 different different JJ cord-319626-f1b3ygg0 241 42 times time NNS cord-319626-f1b3ygg0 241 43 after after IN cord-319626-f1b3ygg0 241 44 infection infection NN cord-319626-f1b3ygg0 241 45 at at IN cord-319626-f1b3ygg0 241 46 the the DT cord-319626-f1b3ygg0 241 47 two two CD cord-319626-f1b3ygg0 241 48 temperatures temperature NNS cord-319626-f1b3ygg0 241 49 . . . cord-319626-f1b3ygg0 242 1 At at IN cord-319626-f1b3ygg0 242 2 5 5 CD cord-319626-f1b3ygg0 242 3 - - SYM cord-319626-f1b3ygg0 242 4 7 7 CD cord-319626-f1b3ygg0 242 5 h h NN cord-319626-f1b3ygg0 242 6 after after IN cord-319626-f1b3ygg0 242 7 infection infection NN cord-319626-f1b3ygg0 242 8 at at IN cord-319626-f1b3ygg0 242 9 37 37 CD cord-319626-f1b3ygg0 242 10 ° ° NN cord-319626-f1b3ygg0 242 11 C c NN cord-319626-f1b3ygg0 242 12 , , , cord-319626-f1b3ygg0 242 13 the the DT cord-319626-f1b3ygg0 242 14 rough rough JJ cord-319626-f1b3ygg0 242 15 ER er NN cord-319626-f1b3ygg0 242 16 was be VBD cord-319626-f1b3ygg0 242 17 not not RB cord-319626-f1b3ygg0 242 18 labeled label VBN cord-319626-f1b3ygg0 242 19 but but CC cord-319626-f1b3ygg0 242 20 the the DT cord-319626-f1b3ygg0 242 21 budding bud VBG cord-319626-f1b3ygg0 242 22 compartment compartment NN cord-319626-f1b3ygg0 242 23 was be VBD cord-319626-f1b3ygg0 242 24 labeled label VBN cord-319626-f1b3ygg0 242 25 as as IN cord-319626-f1b3ygg0 242 26 expected expect VBN cord-319626-f1b3ygg0 242 27 from from IN cord-319626-f1b3ygg0 242 28 indirect indirect JJ cord-319626-f1b3ygg0 242 29 immunofluorescence immunofluorescence NN cord-319626-f1b3ygg0 242 30 microscopy microscopy NN cord-319626-f1b3ygg0 242 31 . . . cord-319626-f1b3ygg0 243 1 There there EX cord-319626-f1b3ygg0 243 2 was be VBD cord-319626-f1b3ygg0 243 3 also also RB cord-319626-f1b3ygg0 243 4 distinct distinct JJ cord-319626-f1b3ygg0 243 5 labeling labeling NN cord-319626-f1b3ygg0 243 6 of of IN cord-319626-f1b3ygg0 243 7 all all PDT cord-319626-f1b3ygg0 243 8 the the DT cord-319626-f1b3ygg0 243 9 stacked stack VBN cord-319626-f1b3ygg0 243 10 cisternal cisternal JJ cord-319626-f1b3ygg0 243 11 membranes membrane NNS cord-319626-f1b3ygg0 243 12 of of IN cord-319626-f1b3ygg0 243 13 the the DT cord-319626-f1b3ygg0 243 14 Golgi Golgi NNP cord-319626-f1b3ygg0 243 15 complex complex NN cord-319626-f1b3ygg0 243 16 ( ( -LRB- cord-319626-f1b3ygg0 243 17 Fig fig NN cord-319626-f1b3ygg0 243 18 . . . cord-319626-f1b3ygg0 243 19 11 11 CD cord-319626-f1b3ygg0 243 20 ) ) -RRB- cord-319626-f1b3ygg0 243 21 . . . cord-319626-f1b3ygg0 244 1 The the DT cord-319626-f1b3ygg0 244 2 reaction reaction NN cord-319626-f1b3ygg0 244 3 product product NN cord-319626-f1b3ygg0 244 4 was be VBD cord-319626-f1b3ygg0 244 5 found find VBN cord-319626-f1b3ygg0 244 6 on on IN cord-319626-f1b3ygg0 244 7 the the DT cord-319626-f1b3ygg0 244 8 lumenal lumenal JJ cord-319626-f1b3ygg0 244 9 side side NN cord-319626-f1b3ygg0 244 10 of of IN cord-319626-f1b3ygg0 244 11 these these DT cord-319626-f1b3ygg0 244 12 compartments compartment NNS cord-319626-f1b3ygg0 244 13 ( ( -LRB- cord-319626-f1b3ygg0 244 14 Fig fig NN cord-319626-f1b3ygg0 244 15 . . . cord-319626-f1b3ygg0 244 16 11 11 CD cord-319626-f1b3ygg0 244 17 ) ) -RRB- cord-319626-f1b3ygg0 244 18 consistent consistent JJ cord-319626-f1b3ygg0 244 19 with with IN cord-319626-f1b3ygg0 244 20 the the DT cord-319626-f1b3ygg0 244 21 fact fact NN cord-319626-f1b3ygg0 244 22 that that IN cord-319626-f1b3ygg0 244 23 the the DT cord-319626-f1b3ygg0 244 24 mAb mAb NNS cord-319626-f1b3ygg0 244 25 recognizes recognize VBZ cord-319626-f1b3ygg0 244 26 an an DT cord-319626-f1b3ygg0 244 27 amino amino NN cord-319626-f1b3ygg0 244 28 - - HYPH cord-319626-f1b3ygg0 244 29 terminal terminal NN cord-319626-f1b3ygg0 244 30 epitope epitope NN cord-319626-f1b3ygg0 244 31 of of IN cord-319626-f1b3ygg0 244 32 El El NNP cord-319626-f1b3ygg0 244 33 . . . cord-319626-f1b3ygg0 245 1 Continued continue VBN cord-319626-f1b3ygg0 245 2 incubation incubation NN cord-319626-f1b3ygg0 245 3 at at IN cord-319626-f1b3ygg0 245 4 37 37 CD cord-319626-f1b3ygg0 245 5 ° ° NNS cord-319626-f1b3ygg0 246 1 C C NNP cord-319626-f1b3ygg0 246 2 increased increase VBD cord-319626-f1b3ygg0 246 3 the the DT cord-319626-f1b3ygg0 246 4 number number NN cord-319626-f1b3ygg0 246 5 of of IN cord-319626-f1b3ygg0 246 6 labeled label VBN cord-319626-f1b3ygg0 246 7 compartments compartment NNS cord-319626-f1b3ygg0 246 8 to to TO cord-319626-f1b3ygg0 246 9 include include VB cord-319626-f1b3ygg0 246 10 the the DT cord-319626-f1b3ygg0 246 11 rough rough JJ cord-319626-f1b3ygg0 246 12 ER er NN cord-319626-f1b3ygg0 246 13 ( ( -LRB- cord-319626-f1b3ygg0 246 14 Tooze Tooze NNP cord-319626-f1b3ygg0 246 15 et et NNP cord-319626-f1b3ygg0 246 16 al al NNP cord-319626-f1b3ygg0 246 17 . . . cord-319626-f1b3ygg0 246 18 1985 1985 CD cord-319626-f1b3ygg0 246 19 ) ) -RRB- cord-319626-f1b3ygg0 246 20 . . . cord-319626-f1b3ygg0 247 1 The the DT cord-319626-f1b3ygg0 247 2 labeling labeling NN cord-319626-f1b3ygg0 247 3 of of IN cord-319626-f1b3ygg0 247 4 Golgi Golgi NNP cord-319626-f1b3ygg0 247 5 cisternae cisternae NNP cord-319626-f1b3ygg0 247 6 at at IN cord-319626-f1b3ygg0 247 7 5 5 CD cord-319626-f1b3ygg0 247 8 - - SYM cord-319626-f1b3ygg0 247 9 7 7 CD cord-319626-f1b3ygg0 247 10 h h NN cord-319626-f1b3ygg0 247 11 after after IN cord-319626-f1b3ygg0 247 12 infection infection NN cord-319626-f1b3ygg0 247 13 indicates indicate VBZ cord-319626-f1b3ygg0 247 14 that that IN cord-319626-f1b3ygg0 247 15 some some DT cord-319626-f1b3ygg0 247 16 E1 e1 NN cord-319626-f1b3ygg0 247 17 reaches reach VBZ cord-319626-f1b3ygg0 247 18 the the DT cord-319626-f1b3ygg0 247 19 cisternal cisternal JJ cord-319626-f1b3ygg0 247 20 membranes membrane NNS cord-319626-f1b3ygg0 247 21 as as IN cord-319626-f1b3ygg0 247 22 an an DT cord-319626-f1b3ygg0 247 23 integral integral JJ cord-319626-f1b3ygg0 247 24 membrane membrane NN cord-319626-f1b3ygg0 247 25 protein protein NN cord-319626-f1b3ygg0 247 26 . . . cord-319626-f1b3ygg0 248 1 However however RB cord-319626-f1b3ygg0 248 2 , , , cord-319626-f1b3ygg0 248 3 the the DT cord-319626-f1b3ygg0 248 4 absence absence NN cord-319626-f1b3ygg0 248 5 of of IN cord-319626-f1b3ygg0 248 6 budding bud VBG cord-319626-f1b3ygg0 248 7 of of IN cord-319626-f1b3ygg0 248 8 virions virion NNS cord-319626-f1b3ygg0 248 9 directly directly RB cord-319626-f1b3ygg0 248 10 into into IN cord-319626-f1b3ygg0 248 11 the the DT cord-319626-f1b3ygg0 248 12 Golgi Golgi NNP cord-319626-f1b3ygg0 248 13 cisternae cisternae NNP cord-319626-f1b3ygg0 248 14 at at IN cord-319626-f1b3ygg0 248 15 these these DT cord-319626-f1b3ygg0 248 16 early early JJ cord-319626-f1b3ygg0 248 17 times time NNS cord-319626-f1b3ygg0 248 18 after after IN cord-319626-f1b3ygg0 248 19 infection infection NN cord-319626-f1b3ygg0 248 20 ( ( -LRB- cord-319626-f1b3ygg0 248 21 Tooze Tooze NNP cord-319626-f1b3ygg0 248 22 et et NNP cord-319626-f1b3ygg0 248 23 al al NNP cord-319626-f1b3ygg0 248 24 . . NNP cord-319626-f1b3ygg0 248 25 , , , cord-319626-f1b3ygg0 248 26 1984 1984 CD cord-319626-f1b3ygg0 248 27 ; ; : cord-319626-f1b3ygg0 248 28 Figs fig NNS cord-319626-f1b3ygg0 248 29 . . . cord-319626-f1b3ygg0 248 30 9 9 CD cord-319626-f1b3ygg0 248 31 and and CC cord-319626-f1b3ygg0 248 32 11 11 CD cord-319626-f1b3ygg0 248 33 ) ) -RRB- cord-319626-f1b3ygg0 248 34 indicates indicate VBZ cord-319626-f1b3ygg0 248 35 that that IN cord-319626-f1b3ygg0 248 36 the the DT cord-319626-f1b3ygg0 248 37 amount amount NN cord-319626-f1b3ygg0 248 38 of of IN cord-319626-f1b3ygg0 248 39 E1 e1 NN cord-319626-f1b3ygg0 248 40 in in IN cord-319626-f1b3ygg0 248 41 these these DT cord-319626-f1b3ygg0 248 42 membranes membrane NNS cord-319626-f1b3ygg0 248 43 is be VBZ cord-319626-f1b3ygg0 248 44 below below IN cord-319626-f1b3ygg0 248 45 the the DT cord-319626-f1b3ygg0 248 46 threshold threshold NN cord-319626-f1b3ygg0 248 47 concentration concentration NN cord-319626-f1b3ygg0 248 48 necessary necessary JJ cord-319626-f1b3ygg0 248 49 for for IN cord-319626-f1b3ygg0 248 50 budding bud VBG cord-319626-f1b3ygg0 248 51 but but CC cord-319626-f1b3ygg0 248 52 clearly clearly RB cord-319626-f1b3ygg0 248 53 is be VBZ cord-319626-f1b3ygg0 248 54 not not RB cord-319626-f1b3ygg0 248 55 below below IN cord-319626-f1b3ygg0 248 56 the the DT cord-319626-f1b3ygg0 248 57 threshold threshold NN cord-319626-f1b3ygg0 248 58 of of IN cord-319626-f1b3ygg0 248 59 detection detection NN cord-319626-f1b3ygg0 248 60 by by IN cord-319626-f1b3ygg0 248 61 the the DT cord-319626-f1b3ygg0 248 62 immunoperoxidase immunoperoxidase NN cord-319626-f1b3ygg0 248 63 technique technique NN cord-319626-f1b3ygg0 248 64 which which WDT cord-319626-f1b3ygg0 248 65 involves involve VBZ cord-319626-f1b3ygg0 248 66 enzymatic enzymatic JJ cord-319626-f1b3ygg0 248 67 amplification amplification NN cord-319626-f1b3ygg0 248 68 . . . cord-319626-f1b3ygg0 249 1 After after IN cord-319626-f1b3ygg0 249 2 a a DT cord-319626-f1b3ygg0 249 3 shift shift NN cord-319626-f1b3ygg0 249 4 from from IN cord-319626-f1b3ygg0 249 5 37 37 CD cord-319626-f1b3ygg0 249 6 to to TO cord-319626-f1b3ygg0 249 7 31 31 CD cord-319626-f1b3ygg0 249 8 ° ° NN cord-319626-f1b3ygg0 249 9 C c NN cord-319626-f1b3ygg0 249 10 at at IN cord-319626-f1b3ygg0 249 11 times time NNS cord-319626-f1b3ygg0 249 12 between between IN cord-319626-f1b3ygg0 249 13 5 5 CD cord-319626-f1b3ygg0 249 14 and and CC cord-319626-f1b3ygg0 249 15 7 7 CD cord-319626-f1b3ygg0 249 16 h h NN cord-319626-f1b3ygg0 249 17 after after IN cord-319626-f1b3ygg0 249 18 infection infection NN cord-319626-f1b3ygg0 249 19 , , , cord-319626-f1b3ygg0 249 20 with with IN cord-319626-f1b3ygg0 249 21 3 3 CD cord-319626-f1b3ygg0 249 22 - - SYM cord-319626-f1b3ygg0 249 23 5 5 CD cord-319626-f1b3ygg0 249 24 h h NN cord-319626-f1b3ygg0 249 25 further further JJ cord-319626-f1b3ygg0 249 26 incubation incubation NN cord-319626-f1b3ygg0 249 27 at at IN cord-319626-f1b3ygg0 249 28 the the DT cord-319626-f1b3ygg0 249 29 lower low JJR cord-319626-f1b3ygg0 249 30 temperature temperature NN cord-319626-f1b3ygg0 249 31 , , , cord-319626-f1b3ygg0 249 32 the the DT cord-319626-f1b3ygg0 249 33 labeling labeling NN cord-319626-f1b3ygg0 249 34 pattern pattern NN cord-319626-f1b3ygg0 249 35 of of IN cord-319626-f1b3ygg0 249 36 E1 e1 NN cord-319626-f1b3ygg0 249 37 using use VBG cord-319626-f1b3ygg0 249 38 the the DT cord-319626-f1b3ygg0 249 39 im- im- NN cord-319626-f1b3ygg0 249 40 to to TO cord-319626-f1b3ygg0 249 41 show show VB cord-319626-f1b3ygg0 249 42 budding budding JJ cord-319626-f1b3ygg0 249 43 compartments compartment NNS cord-319626-f1b3ygg0 249 44 not not RB cord-319626-f1b3ygg0 249 45 juxtaposed juxtaposed JJ cord-319626-f1b3ygg0 249 46 to to IN cord-319626-f1b3ygg0 249 47 Golgi Golgi NNP cord-319626-f1b3ygg0 249 48 stacks stack NNS cord-319626-f1b3ygg0 249 49 and and CC cord-319626-f1b3ygg0 249 50 therefore therefore RB cord-319626-f1b3ygg0 249 51 are be VBP cord-319626-f1b3ygg0 249 52 comparable comparable JJ cord-319626-f1b3ygg0 249 53 to to IN cord-319626-f1b3ygg0 249 54 the the DT cord-319626-f1b3ygg0 249 55 budding bud VBG cord-319626-f1b3ygg0 249 56 compartment compartment NN cord-319626-f1b3ygg0 249 57 in in IN cord-319626-f1b3ygg0 249 58 an an DT cord-319626-f1b3ygg0 249 59 uninfected uninfected JJ cord-319626-f1b3ygg0 249 60 cell cell NN cord-319626-f1b3ygg0 249 61 shown show VBN cord-319626-f1b3ygg0 249 62 in in IN cord-319626-f1b3ygg0 249 63 Fig Fig NNP cord-319626-f1b3ygg0 249 64 . . . cord-319626-f1b3ygg0 250 1 9 9 LS cord-319626-f1b3ygg0 250 2 . . . cord-319626-f1b3ygg0 251 1 ( ( -LRB- cord-319626-f1b3ygg0 251 2 Budding bud VBG cord-319626-f1b3ygg0 251 3 compartments compartment NNS cord-319626-f1b3ygg0 251 4 close close RB cord-319626-f1b3ygg0 251 5 to to IN cord-319626-f1b3ygg0 251 6 Golgi Golgi NNP cord-319626-f1b3ygg0 251 7 stacks stack NNS cord-319626-f1b3ygg0 251 8 are be VBP cord-319626-f1b3ygg0 251 9 shown show VBN cord-319626-f1b3ygg0 251 10 in in IN cord-319626-f1b3ygg0 251 11 Fig Fig NNP cord-319626-f1b3ygg0 251 12 . . . cord-319626-f1b3ygg0 252 1 11 11 CD cord-319626-f1b3ygg0 252 2 . . . cord-319626-f1b3ygg0 252 3 ) ) -RRB- cord-319626-f1b3ygg0 253 1 Note note VB cord-319626-f1b3ygg0 253 2 the the DT cord-319626-f1b3ygg0 253 3 irregular irregular JJ cord-319626-f1b3ygg0 253 4 form form NN cord-319626-f1b3ygg0 253 5 of of IN cord-319626-f1b3ygg0 253 6 this this DT cord-319626-f1b3ygg0 253 7 compartment compartment NN cord-319626-f1b3ygg0 253 8 and and CC cord-319626-f1b3ygg0 253 9 its -PRON- PRP$ cord-319626-f1b3ygg0 253 10 distension distension NN cord-319626-f1b3ygg0 253 11 by by IN cord-319626-f1b3ygg0 253 12 the the DT cord-319626-f1b3ygg0 253 13 accumulating accumulate VBG cord-319626-f1b3ygg0 253 14 virions virion NNS cord-319626-f1b3ygg0 253 15 . . . cord-319626-f1b3ygg0 254 1 Frequently frequently RB cord-319626-f1b3ygg0 254 2 parts part NNS cord-319626-f1b3ygg0 254 3 of of IN cord-319626-f1b3ygg0 254 4 the the DT cord-319626-f1b3ygg0 254 5 cytoplasmic cytoplasmic JJ cord-319626-f1b3ygg0 254 6 face face NN cord-319626-f1b3ygg0 254 7 of of IN cord-319626-f1b3ygg0 254 8 the the DT cord-319626-f1b3ygg0 254 9 budding bud VBG cord-319626-f1b3ygg0 254 10 compartment compartment NN cord-319626-f1b3ygg0 254 11 have have VBP cord-319626-f1b3ygg0 254 12 a a DT cord-319626-f1b3ygg0 254 13 " " `` cord-319626-f1b3ygg0 254 14 bristle bristle NN cord-319626-f1b3ygg0 254 15 " " '' cord-319626-f1b3ygg0 254 16 coat coat NN cord-319626-f1b3ygg0 254 17 and and CC cord-319626-f1b3ygg0 254 18 appear appear VB cord-319626-f1b3ygg0 254 19 to to TO cord-319626-f1b3ygg0 254 20 be be VB cord-319626-f1b3ygg0 254 21 sites site NNS cord-319626-f1b3ygg0 254 22 of of IN cord-319626-f1b3ygg0 254 23 fusion fusion NN cord-319626-f1b3ygg0 254 24 of of IN cord-319626-f1b3ygg0 254 25 small small JJ cord-319626-f1b3ygg0 254 26 vesicles vesicle NNS cord-319626-f1b3ygg0 254 27 ( ( -LRB- cord-319626-f1b3ygg0 254 28 arrowheads arrowhead NNS cord-319626-f1b3ygg0 254 29 in in IN cord-319626-f1b3ygg0 254 30 A a NN cord-319626-f1b3ygg0 254 31 , , , cord-319626-f1b3ygg0 254 32 B b NN cord-319626-f1b3ygg0 254 33 , , , cord-319626-f1b3ygg0 254 34 D D NNP cord-319626-f1b3ygg0 254 35 , , , cord-319626-f1b3ygg0 254 36 and and CC cord-319626-f1b3ygg0 254 37 E e LS cord-319626-f1b3ygg0 254 38 ) ) -RRB- cord-319626-f1b3ygg0 254 39 . . . cord-319626-f1b3ygg0 255 1 This this DT cord-319626-f1b3ygg0 255 2 coat coat NN cord-319626-f1b3ygg0 255 3 differs differ VBZ cord-319626-f1b3ygg0 255 4 in in IN cord-319626-f1b3ygg0 255 5 morphology morphology NN cord-319626-f1b3ygg0 255 6 from from IN cord-319626-f1b3ygg0 255 7 the the DT cord-319626-f1b3ygg0 255 8 coats coat NNS cord-319626-f1b3ygg0 255 9 of of IN cord-319626-f1b3ygg0 255 10 typical typical JJ cord-319626-f1b3ygg0 255 11 coated coated JJ cord-319626-f1b3ygg0 255 12 vesicles vesicle NNS cord-319626-f1b3ygg0 255 13 ( ( -LRB- cord-319626-f1b3ygg0 255 14 open open JJ cord-319626-f1b3ygg0 255 15 arrows arrow NNS cord-319626-f1b3ygg0 255 16 in in IN cord-319626-f1b3ygg0 255 17 A a NN cord-319626-f1b3ygg0 255 18 and and CC cord-319626-f1b3ygg0 255 19 C C NNP cord-319626-f1b3ygg0 255 20 ) ) -RRB- cord-319626-f1b3ygg0 255 21 . . . cord-319626-f1b3ygg0 256 1 Orci Orci NNP cord-319626-f1b3ygg0 256 2 et et NNP cord-319626-f1b3ygg0 256 3 al al NNP cord-319626-f1b3ygg0 256 4 . . . cord-319626-f1b3ygg0 257 1 ( ( -LRB- cord-319626-f1b3ygg0 257 2 1986 1986 CD cord-319626-f1b3ygg0 257 3 ) ) -RRB- cord-319626-f1b3ygg0 257 4 have have VBP cord-319626-f1b3ygg0 257 5 shown show VBN cord-319626-f1b3ygg0 257 6 by by IN cord-319626-f1b3ygg0 257 7 immunocytochemistry immunocytochemistry NN cord-319626-f1b3ygg0 257 8 that that IN cord-319626-f1b3ygg0 257 9 coats coat NNS cord-319626-f1b3ygg0 257 10 of of IN cord-319626-f1b3ygg0 257 11 this this DT cord-319626-f1b3ygg0 257 12 type type NN cord-319626-f1b3ygg0 257 13 associated associate VBN cord-319626-f1b3ygg0 257 14 with with IN cord-319626-f1b3ygg0 257 15 Golgi Golgi NNP cord-319626-f1b3ygg0 257 16 membranes membrane NNS cord-319626-f1b3ygg0 257 17 lack lack VBP cord-319626-f1b3ygg0 257 18 clathrin clathrin NN cord-319626-f1b3ygg0 257 19 . . . cord-319626-f1b3ygg0 258 1 Bars bar NNS cord-319626-f1b3ygg0 258 2 , , , cord-319626-f1b3ygg0 258 3 0.25 0.25 CD cord-319626-f1b3ygg0 258 4 vtm vtm NNP cord-319626-f1b3ygg0 258 5 . . . cord-319626-f1b3ygg0 259 1 The the DT cord-319626-f1b3ygg0 259 2 Journal Journal NNP cord-319626-f1b3ygg0 259 3 of of IN cord-319626-f1b3ygg0 259 4 Cell Cell NNP cord-319626-f1b3ygg0 259 5 Biology Biology NNP cord-319626-f1b3ygg0 259 6 , , , cord-319626-f1b3ygg0 259 7 Volume volume NN cord-319626-f1b3ygg0 259 8 106 106 CD cord-319626-f1b3ygg0 259 9 , , , cord-319626-f1b3ygg0 259 10 1988 1988 CD cord-319626-f1b3ygg0 259 11 Figure figure NN cord-319626-f1b3ygg0 259 12 9 9 CD cord-319626-f1b3ygg0 259 13 . . . cord-319626-f1b3ygg0 260 1 Serial serial JJ cord-319626-f1b3ygg0 260 2 sections section NNS cord-319626-f1b3ygg0 260 3 through through IN cord-319626-f1b3ygg0 260 4 a a DT cord-319626-f1b3ygg0 260 5 budding bud VBG cord-319626-f1b3ygg0 260 6 compartment compartment NN cord-319626-f1b3ygg0 260 7 in in IN cord-319626-f1b3ygg0 260 8 an an DT cord-319626-f1b3ygg0 260 9 uninfected uninfected JJ cord-319626-f1b3ygg0 260 10 sac(- sac(- NNS cord-319626-f1b3ygg0 260 11 ) ) -RRB- cord-319626-f1b3ygg0 260 12 cell cell NN cord-319626-f1b3ygg0 260 13 . . . cord-319626-f1b3ygg0 261 1 In in IN cord-319626-f1b3ygg0 261 2 uninfected uninfected JJ cord-319626-f1b3ygg0 261 3 cells cell NNS cord-319626-f1b3ygg0 261 4 this this DT cord-319626-f1b3ygg0 261 5 compartment compartment NN cord-319626-f1b3ygg0 261 6 ( ( -LRB- cord-319626-f1b3ygg0 261 7 arrowheads arrowhead NNS cord-319626-f1b3ygg0 261 8 ) ) -RRB- cord-319626-f1b3ygg0 261 9 is be VBZ cord-319626-f1b3ygg0 261 10 found find VBN cord-319626-f1b3ygg0 261 11 closely closely RB cord-319626-f1b3ygg0 261 12 associated associate VBN cord-319626-f1b3ygg0 261 13 with with IN cord-319626-f1b3ygg0 261 14 transitional transitional JJ cord-319626-f1b3ygg0 261 15 element element NN cord-319626-f1b3ygg0 261 16 regions region NNS cord-319626-f1b3ygg0 261 17 of of IN cord-319626-f1b3ygg0 261 18 the the DT cord-319626-f1b3ygg0 261 19 rough rough JJ cord-319626-f1b3ygg0 261 20 ER er NN cord-319626-f1b3ygg0 261 21 ( ( -LRB- cord-319626-f1b3ygg0 261 22 arrows arrow NNS cord-319626-f1b3ygg0 261 23 ) ) -RRB- cord-319626-f1b3ygg0 261 24 . . . cord-319626-f1b3ygg0 262 1 Often often RB cord-319626-f1b3ygg0 262 2 the the DT cord-319626-f1b3ygg0 262 3 budding bud VBG cord-319626-f1b3ygg0 262 4 compartment compartment NN cord-319626-f1b3ygg0 262 5 occurs occur VBZ cord-319626-f1b3ygg0 262 6 between between IN cord-319626-f1b3ygg0 262 7 transitional transitional JJ cord-319626-f1b3ygg0 262 8 elements element NNS cord-319626-f1b3ygg0 262 9 and and CC cord-319626-f1b3ygg0 262 10 Golgi Golgi NNP cord-319626-f1b3ygg0 262 11 stacks stack NNS cord-319626-f1b3ygg0 262 12 but but CC cord-319626-f1b3ygg0 262 13 sometimes sometimes RB cord-319626-f1b3ygg0 262 14 , , , cord-319626-f1b3ygg0 262 15 as as IN cord-319626-f1b3ygg0 262 16 this this DT cord-319626-f1b3ygg0 262 17 figure figure NN cord-319626-f1b3ygg0 262 18 shows show VBZ cord-319626-f1b3ygg0 262 19 , , , cord-319626-f1b3ygg0 262 20 it -PRON- PRP cord-319626-f1b3ygg0 262 21 is be VBZ cord-319626-f1b3ygg0 262 22 not not RB cord-319626-f1b3ygg0 262 23 adjacent adjacent JJ cord-319626-f1b3ygg0 262 24 to to IN cord-319626-f1b3ygg0 262 25 a a DT cord-319626-f1b3ygg0 262 26 Golgi Golgi NNP cord-319626-f1b3ygg0 262 27 stack stack NN cord-319626-f1b3ygg0 262 28 . . . cord-319626-f1b3ygg0 263 1 In in IN cord-319626-f1b3ygg0 263 2 this this DT cord-319626-f1b3ygg0 263 3 situation situation NN cord-319626-f1b3ygg0 263 4 , , , cord-319626-f1b3ygg0 263 5 because because IN cord-319626-f1b3ygg0 263 6 of of IN cord-319626-f1b3ygg0 263 7 the the DT cord-319626-f1b3ygg0 263 8 absence absence NN cord-319626-f1b3ygg0 263 9 of of IN cord-319626-f1b3ygg0 263 10 smooth smooth JJ cord-319626-f1b3ygg0 263 11 cis cis NNP cord-319626-f1b3ygg0 263 12 - - HYPH cord-319626-f1b3ygg0 263 13 Golgi Golgi NNP cord-319626-f1b3ygg0 263 14 membranes membrane NNS cord-319626-f1b3ygg0 263 15 , , , cord-319626-f1b3ygg0 263 16 it -PRON- PRP cord-319626-f1b3ygg0 263 17 is be VBZ cord-319626-f1b3ygg0 263 18 easier easy JJR cord-319626-f1b3ygg0 263 19 to to TO cord-319626-f1b3ygg0 263 20 trace trace VB cord-319626-f1b3ygg0 263 21 the the DT cord-319626-f1b3ygg0 263 22 budding bud VBG cord-319626-f1b3ygg0 263 23 compartment compartment NN cord-319626-f1b3ygg0 263 24 through through IN cord-319626-f1b3ygg0 263 25 serial serial JJ cord-319626-f1b3ygg0 263 26 sections section NNS cord-319626-f1b3ygg0 263 27 . . . cord-319626-f1b3ygg0 264 1 Note note VB cord-319626-f1b3ygg0 264 2 the the DT cord-319626-f1b3ygg0 264 3 complex complex JJ cord-319626-f1b3ygg0 264 4 irregular irregular JJ cord-319626-f1b3ygg0 264 5 morphology morphology NN cord-319626-f1b3ygg0 264 6 of of IN cord-319626-f1b3ygg0 264 7 the the DT cord-319626-f1b3ygg0 264 8 budding bud VBG cord-319626-f1b3ygg0 264 9 compartment compartment NN cord-319626-f1b3ygg0 264 10 and and CC cord-319626-f1b3ygg0 264 11 the the DT cord-319626-f1b3ygg0 264 12 absence absence NN cord-319626-f1b3ygg0 264 13 of of IN cord-319626-f1b3ygg0 264 14 ribosomes ribosome NNS cord-319626-f1b3ygg0 264 15 from from IN cord-319626-f1b3ygg0 264 16 its -PRON- PRP$ cord-319626-f1b3ygg0 264 17 membrane membrane NN cord-319626-f1b3ygg0 264 18 . . . cord-319626-f1b3ygg0 265 1 The the DT cord-319626-f1b3ygg0 265 2 micrographs micrograph NNS cord-319626-f1b3ygg0 265 3 shown show VBN cord-319626-f1b3ygg0 265 4 are be VBP cord-319626-f1b3ygg0 265 5 of of IN cord-319626-f1b3ygg0 265 6 nine nine CD cord-319626-f1b3ygg0 265 7 sections section NNS cord-319626-f1b3ygg0 265 8 ( ( -LRB- cord-319626-f1b3ygg0 265 9 SI-$5 si-$5 NN cord-319626-f1b3ygg0 265 10 , , , cord-319626-f1b3ygg0 265 11 $ $ $ cord-319626-f1b3ygg0 265 12 7 7 CD cord-319626-f1b3ygg0 265 13 , , , cord-319626-f1b3ygg0 265 14 and and CC cord-319626-f1b3ygg0 265 15 $ $ $ cord-319626-f1b3ygg0 265 16 I0-S12 I0-S12 NNP cord-319626-f1b3ygg0 265 17 ) ) -RRB- cord-319626-f1b3ygg0 265 18 from from IN cord-319626-f1b3ygg0 265 19 a a DT cord-319626-f1b3ygg0 265 20 continuous continuous JJ cord-319626-f1b3ygg0 265 21 ribbon ribbon NN cord-319626-f1b3ygg0 265 22 of of IN cord-319626-f1b3ygg0 265 23 13 13 CD cord-319626-f1b3ygg0 265 24 sections section NNS cord-319626-f1b3ygg0 265 25 , , , cord-319626-f1b3ygg0 265 26 each each DT cord-319626-f1b3ygg0 265 27 , , , cord-319626-f1b3ygg0 265 28 ~50 ~50 XX cord-319626-f1b3ygg0 265 29 - - SYM cord-319626-f1b3ygg0 265 30 60 60 CD cord-319626-f1b3ygg0 265 31 nm nm NN cord-319626-f1b3ygg0 265 32 thick thick JJ cord-319626-f1b3ygg0 265 33 . . . cord-319626-f1b3ygg0 266 1 In in IN cord-319626-f1b3ygg0 266 2 this this DT cord-319626-f1b3ygg0 266 3 example example NN cord-319626-f1b3ygg0 266 4 , , , cord-319626-f1b3ygg0 266 5 therefore therefore RB cord-319626-f1b3ygg0 266 6 , , , cord-319626-f1b3ygg0 266 7 the the DT cord-319626-f1b3ygg0 266 8 budding bud VBG cord-319626-f1b3ygg0 266 9 compartment compartment NN cord-319626-f1b3ygg0 266 10 extended extend VBN cord-319626-f1b3ygg0 266 11 through through IN cord-319626-f1b3ygg0 266 12 a a DT cord-319626-f1b3ygg0 266 13 depth depth NN cord-319626-f1b3ygg0 266 14 of of IN cord-319626-f1b3ygg0 266 15 , , , cord-319626-f1b3ygg0 266 16 , , , cord-319626-f1b3ygg0 266 17 , , , cord-319626-f1b3ygg0 266 18 650 650 CD cord-319626-f1b3ygg0 266 19 nm nm NN cord-319626-f1b3ygg0 266 20 . . . cord-319626-f1b3ygg0 267 1 All all DT cord-319626-f1b3ygg0 267 2 micrographs micrograph NNS cord-319626-f1b3ygg0 267 3 are be VBP cord-319626-f1b3ygg0 267 4 at at IN cord-319626-f1b3ygg0 267 5 the the DT cord-319626-f1b3ygg0 267 6 same same JJ cord-319626-f1b3ygg0 267 7 magnification magnification NN cord-319626-f1b3ygg0 267 8 . . . cord-319626-f1b3ygg0 268 1 Bar bar NN cord-319626-f1b3ygg0 268 2 , , , cord-319626-f1b3ygg0 268 3 0.25 0.25 CD cord-319626-f1b3ygg0 268 4 lam lam NN cord-319626-f1b3ygg0 268 5 . . . cord-319626-f1b3ygg0 269 1 munoperoxidase munoperoxidase NNP cord-319626-f1b3ygg0 269 2 technique technique NN cord-319626-f1b3ygg0 269 3 was be VBD cord-319626-f1b3ygg0 269 4 very very RB cord-319626-f1b3ygg0 269 5 similar similar JJ cord-319626-f1b3ygg0 269 6 to to IN cord-319626-f1b3ygg0 269 7 that that DT cord-319626-f1b3ygg0 269 8 seen see VBN cord-319626-f1b3ygg0 269 9 at at IN cord-319626-f1b3ygg0 269 10 7 7 CD cord-319626-f1b3ygg0 269 11 h h NN cord-319626-f1b3ygg0 269 12 after after IN cord-319626-f1b3ygg0 269 13 infection infection NN cord-319626-f1b3ygg0 269 14 at at IN cord-319626-f1b3ygg0 269 15 37 37 CD cord-319626-f1b3ygg0 269 16 ° ° NN cord-319626-f1b3ygg0 269 17 C c NN cord-319626-f1b3ygg0 269 18 . . . cord-319626-f1b3ygg0 270 1 Reaction reaction NN cord-319626-f1b3ygg0 270 2 product product NN cord-319626-f1b3ygg0 270 3 was be VBD cord-319626-f1b3ygg0 270 4 seen see VBN cord-319626-f1b3ygg0 270 5 in in IN cord-319626-f1b3ygg0 270 6 the the DT cord-319626-f1b3ygg0 270 7 budding bud VBG cord-319626-f1b3ygg0 270 8 compartment compartment NN cord-319626-f1b3ygg0 270 9 and and CC cord-319626-f1b3ygg0 270 10 some some DT cord-319626-f1b3ygg0 270 11 in in IN cord-319626-f1b3ygg0 270 12 the the DT cord-319626-f1b3ygg0 270 13 Golgi Golgi NNP cord-319626-f1b3ygg0 270 14 cisternae cisternae NNP cord-319626-f1b3ygg0 270 15 ( ( -LRB- cord-319626-f1b3ygg0 270 16 data datum NNS cord-319626-f1b3ygg0 270 17 not not RB cord-319626-f1b3ygg0 270 18 shown show VBN cord-319626-f1b3ygg0 270 19 ) ) -RRB- cord-319626-f1b3ygg0 270 20 . . . cord-319626-f1b3ygg0 271 1 There there EX cord-319626-f1b3ygg0 271 2 are be VBP cord-319626-f1b3ygg0 271 3 at at RB cord-319626-f1b3ygg0 271 4 least least RBS cord-319626-f1b3ygg0 271 5 two two CD cord-319626-f1b3ygg0 271 6 posttranslational posttranslational JJ cord-319626-f1b3ygg0 271 7 modifications modification NNS cord-319626-f1b3ygg0 271 8 which which WDT cord-319626-f1b3ygg0 271 9 result result VBP cord-319626-f1b3ygg0 271 10 in in IN cord-319626-f1b3ygg0 271 11 a a DT cord-319626-f1b3ygg0 271 12 change change NN cord-319626-f1b3ygg0 271 13 in in IN cord-319626-f1b3ygg0 271 14 the the DT cord-319626-f1b3ygg0 271 15 molecular molecular JJ cord-319626-f1b3ygg0 271 16 mass mass NN cord-319626-f1b3ygg0 271 17 of of IN cord-319626-f1b3ygg0 271 18 coronavirus coronavirus NN cord-319626-f1b3ygg0 271 19 El El NNP cord-319626-f1b3ygg0 271 20 . . . cord-319626-f1b3ygg0 272 1 The the DT cord-319626-f1b3ygg0 272 2 first first JJ cord-319626-f1b3ygg0 272 3 involves involve VBZ cord-319626-f1b3ygg0 272 4 addition addition NN cord-319626-f1b3ygg0 272 5 of of IN cord-319626-f1b3ygg0 272 6 GalNAc GalNAc NNP cord-319626-f1b3ygg0 272 7 ~10 ~10 . cord-319626-f1b3ygg0 273 1 min min NNP cord-319626-f1b3ygg0 273 2 after after IN cord-319626-f1b3ygg0 273 3 synthe synthe NNP cord-319626-f1b3ygg0 273 4 - - HYPH cord-319626-f1b3ygg0 273 5 Sis Sis NNP cord-319626-f1b3ygg0 273 6 ; ; : cord-319626-f1b3ygg0 273 7 the the DT cord-319626-f1b3ygg0 273 8 molecular molecular JJ cord-319626-f1b3ygg0 273 9 mass mass NN cord-319626-f1b3ygg0 273 10 increase increase NN cord-319626-f1b3ygg0 273 11 of of IN cord-319626-f1b3ygg0 273 12 1 1 CD cord-319626-f1b3ygg0 273 13 kD kd XX cord-319626-f1b3ygg0 273 14 would would MD cord-319626-f1b3ygg0 273 15 correspond correspond VB cord-319626-f1b3ygg0 273 16 to to IN cord-319626-f1b3ygg0 273 17 the the DT cord-319626-f1b3ygg0 273 18 addition addition NN cord-319626-f1b3ygg0 273 19 of of IN cord-319626-f1b3ygg0 273 20 three three CD cord-319626-f1b3ygg0 273 21 GaINAC GaINAC NNP cord-319626-f1b3ygg0 273 22 molecules molecule NNS cord-319626-f1b3ygg0 273 23 . . . cord-319626-f1b3ygg0 274 1 The the DT cord-319626-f1b3ygg0 274 2 sequence sequence NN cord-319626-f1b3ygg0 274 3 of of IN cord-319626-f1b3ygg0 274 4 E1 e1 NN cord-319626-f1b3ygg0 274 5 ( ( -LRB- cord-319626-f1b3ygg0 274 6 Armstrong Armstrong NNP cord-319626-f1b3ygg0 274 7 et et NNP cord-319626-f1b3ygg0 274 8 al al NNP cord-319626-f1b3ygg0 274 9 . . NNP cord-319626-f1b3ygg0 274 10 , , , cord-319626-f1b3ygg0 274 11 1984 1984 CD cord-319626-f1b3ygg0 274 12 ) ) -RRB- cord-319626-f1b3ygg0 274 13 shows show VBZ cord-319626-f1b3ygg0 274 14 that that IN cord-319626-f1b3ygg0 274 15 there there EX cord-319626-f1b3ygg0 274 16 are be VBP cord-319626-f1b3ygg0 274 17 four four CD cord-319626-f1b3ygg0 274 18 potential potential JJ cord-319626-f1b3ygg0 274 19 glycosylation glycosylation NN cord-319626-f1b3ygg0 274 20 sites site NNS cord-319626-f1b3ygg0 274 21 for for IN cord-319626-f1b3ygg0 274 22 O o NN cord-319626-f1b3ygg0 274 23 - - HYPH cord-319626-f1b3ygg0 274 24 linked link VBN cord-319626-f1b3ygg0 274 25 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 274 26 at at IN cord-319626-f1b3ygg0 274 27 the the DT cord-319626-f1b3ygg0 274 28 amino amino NN cord-319626-f1b3ygg0 274 29 terminus terminus NN cord-319626-f1b3ygg0 274 30 of of IN cord-319626-f1b3ygg0 274 31 the the DT cord-319626-f1b3ygg0 274 32 protein protein NN cord-319626-f1b3ygg0 274 33 ( ( -LRB- cord-319626-f1b3ygg0 274 34 NH2-Ser nh2-ser JJ cord-319626-f1b3ygg0 274 35 - - HYPH cord-319626-f1b3ygg0 274 36 Ser Ser NNP cord-319626-f1b3ygg0 274 37 - - HYPH cord-319626-f1b3ygg0 274 38 Thr Thr NNP cord-319626-f1b3ygg0 274 39 - - HYPH cord-319626-f1b3ygg0 274 40 Thr Thr NNP cord-319626-f1b3ygg0 274 41 ) ) -RRB- cord-319626-f1b3ygg0 274 42 of of IN cord-319626-f1b3ygg0 274 43 which which WDT cord-319626-f1b3ygg0 274 44 a a DT cord-319626-f1b3ygg0 274 45 maximum maximum NN cord-319626-f1b3ygg0 274 46 of of IN cord-319626-f1b3ygg0 274 47 three three CD cord-319626-f1b3ygg0 274 48 are be VBP cord-319626-f1b3ygg0 274 49 used use VBN cord-319626-f1b3ygg0 275 1 ( ( -LRB- cord-319626-f1b3ygg0 275 2 Niemann Niemann NNP cord-319626-f1b3ygg0 275 3 et et NNP cord-319626-f1b3ygg0 275 4 al al NNP cord-319626-f1b3ygg0 275 5 . . NNP cord-319626-f1b3ygg0 275 6 , , , cord-319626-f1b3ygg0 275 7 1984 1984 CD cord-319626-f1b3ygg0 275 8 ) ) -RRB- cord-319626-f1b3ygg0 275 9 . . . cord-319626-f1b3ygg0 276 1 About about RB cord-319626-f1b3ygg0 276 2 10 10 CD cord-319626-f1b3ygg0 276 3 min min NN cord-319626-f1b3ygg0 276 4 later later RB cord-319626-f1b3ygg0 276 5 , , , cord-319626-f1b3ygg0 276 6 Gal Gal NNP cord-319626-f1b3ygg0 276 7 and and CC cord-319626-f1b3ygg0 276 8 NANA NANA NNP cord-319626-f1b3ygg0 276 9 are be VBP cord-319626-f1b3ygg0 276 10 added add VBN cord-319626-f1b3ygg0 276 11 to to TO cord-319626-f1b3ygg0 276 12 give give VB cord-319626-f1b3ygg0 276 13 a a DT cord-319626-f1b3ygg0 276 14 variety variety NN cord-319626-f1b3ygg0 276 15 of of IN cord-319626-f1b3ygg0 276 16 mature mature JJ cord-319626-f1b3ygg0 276 17 E1 e1 NN cord-319626-f1b3ygg0 276 18 forms form NNS cord-319626-f1b3ygg0 276 19 which which WDT cord-319626-f1b3ygg0 276 20 can can MD cord-319626-f1b3ygg0 276 21 be be VB cord-319626-f1b3ygg0 276 22 explained explain VBN cord-319626-f1b3ygg0 276 23 by by IN cord-319626-f1b3ygg0 276 24 variation variation NN cord-319626-f1b3ygg0 276 25 in in IN cord-319626-f1b3ygg0 276 26 the the DT cord-319626-f1b3ygg0 276 27 number number NN cord-319626-f1b3ygg0 276 28 of of IN cord-319626-f1b3ygg0 276 29 GalNAc galnac NN cord-319626-f1b3ygg0 276 30 residues residue NNS cord-319626-f1b3ygg0 276 31 that that WDT cord-319626-f1b3ygg0 276 32 are be VBP cord-319626-f1b3ygg0 276 33 modified modify VBN cord-319626-f1b3ygg0 276 34 and and CC cord-319626-f1b3ygg0 276 35 by by IN cord-319626-f1b3ygg0 276 36 the the DT cord-319626-f1b3ygg0 276 37 heterogeneity heterogeneity NN cord-319626-f1b3ygg0 276 38 of of IN cord-319626-f1b3ygg0 276 39 the the DT cord-319626-f1b3ygg0 276 40 mature mature JJ cord-319626-f1b3ygg0 276 41 sialylated sialylate VBN cord-319626-f1b3ygg0 276 42 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 276 43 . . . cord-319626-f1b3ygg0 277 1 The the DT cord-319626-f1b3ygg0 277 2 addition addition NN cord-319626-f1b3ygg0 277 3 of of IN cord-319626-f1b3ygg0 277 4 Gal Gal NNP cord-319626-f1b3ygg0 277 5 and and CC cord-319626-f1b3ygg0 277 6 NANA NANA NNP cord-319626-f1b3ygg0 277 7 probably probably RB cord-319626-f1b3ygg0 277 8 occurs occur VBZ cord-319626-f1b3ygg0 277 9 in in IN cord-319626-f1b3ygg0 277 10 the the DT cord-319626-f1b3ygg0 277 11 trans trans JJ cord-319626-f1b3ygg0 277 12 - - NNP cord-319626-f1b3ygg0 277 13 Golgi Golgi NNP cord-319626-f1b3ygg0 277 14 complex complex NN cord-319626-f1b3ygg0 277 15 , , , cord-319626-f1b3ygg0 277 16 because because IN cord-319626-f1b3ygg0 277 17 the the DT cord-319626-f1b3ygg0 277 18 necessary necessary JJ cord-319626-f1b3ygg0 277 19 enzymes enzyme NNS cord-319626-f1b3ygg0 277 20 are be VBP cord-319626-f1b3ygg0 277 21 found find VBN cord-319626-f1b3ygg0 277 22 there there RB cord-319626-f1b3ygg0 278 1 ( ( -LRB- cord-319626-f1b3ygg0 278 2 Carlson Carlson NNP cord-319626-f1b3ygg0 278 3 et et NNP cord-319626-f1b3ygg0 278 4 al al NNP cord-319626-f1b3ygg0 278 5 . . . cord-319626-f1b3ygg0 279 1 , , , cord-319626-f1b3ygg0 279 2 1973 1973 CD cord-319626-f1b3ygg0 279 3 ; ; : cord-319626-f1b3ygg0 279 4 Sadler Sadler NNP cord-319626-f1b3ygg0 279 5 et et NNP cord-319626-f1b3ygg0 279 6 al al NNP cord-319626-f1b3ygg0 279 7 . . . cord-319626-f1b3ygg0 280 1 , , , cord-319626-f1b3ygg0 280 2 1979 1979 CD cord-319626-f1b3ygg0 280 3 ; ; : cord-319626-f1b3ygg0 280 4 Rearick Rearick NNP cord-319626-f1b3ygg0 280 5 et et NNP cord-319626-f1b3ygg0 280 6 al al NNP cord-319626-f1b3ygg0 280 7 . . NNP cord-319626-f1b3ygg0 280 8 , , , cord-319626-f1b3ygg0 280 9 1979 1979 CD cord-319626-f1b3ygg0 280 10 ; ; . cord-319626-f1b3ygg0 281 1 Elhammer Elhammer NNP cord-319626-f1b3ygg0 281 2 and and CC cord-319626-f1b3ygg0 281 3 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 281 4 , , , cord-319626-f1b3ygg0 281 5 1984 1984 CD cord-319626-f1b3ygg0 281 6 ) ) -RRB- cord-319626-f1b3ygg0 281 7 and and CC cord-319626-f1b3ygg0 281 8 drugs drug NNS cord-319626-f1b3ygg0 281 9 which which WDT cord-319626-f1b3ygg0 281 10 inhibit inhibit VBP cord-319626-f1b3ygg0 281 11 transport transport NN cord-319626-f1b3ygg0 281 12 into into IN cord-319626-f1b3ygg0 281 13 and and CC cord-319626-f1b3ygg0 281 14 through through IN cord-319626-f1b3ygg0 281 15 the the DT cord-319626-f1b3ygg0 281 16 Golgi Golgi NNP cord-319626-f1b3ygg0 281 17 complex complex NN cord-319626-f1b3ygg0 282 1 ( ( -LRB- cord-319626-f1b3ygg0 282 2 Quinn Quinn NNP cord-319626-f1b3ygg0 282 3 et et NNP cord-319626-f1b3ygg0 282 4 al al NNP cord-319626-f1b3ygg0 282 5 . . NNP cord-319626-f1b3ygg0 282 6 , , , cord-319626-f1b3ygg0 282 7 1983 1983 CD cord-319626-f1b3ygg0 283 1 ; ; : cord-319626-f1b3ygg0 283 2 Tartakoff Tartakoff NNP cord-319626-f1b3ygg0 283 3 et et NNP cord-319626-f1b3ygg0 283 4 al al NNP cord-319626-f1b3ygg0 283 5 . . NNP cord-319626-f1b3ygg0 283 6 , , , cord-319626-f1b3ygg0 283 7 1983 1983 CD cord-319626-f1b3ygg0 283 8 ) ) -RRB- cord-319626-f1b3ygg0 284 1 inhibit inhibit VB cord-319626-f1b3ygg0 284 2 formation formation NN cord-319626-f1b3ygg0 284 3 of of IN cord-319626-f1b3ygg0 284 4 the the DT cord-319626-f1b3ygg0 284 5 mature mature JJ cord-319626-f1b3ygg0 284 6 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 284 7 structure structure NN cord-319626-f1b3ygg0 284 8 . . . cord-319626-f1b3ygg0 285 1 We -PRON- PRP cord-319626-f1b3ygg0 285 2 have have VBP cord-319626-f1b3ygg0 285 3 found find VBN cord-319626-f1b3ygg0 285 4 that that IN cord-319626-f1b3ygg0 285 5 both both DT cord-319626-f1b3ygg0 285 6 monensin monensin NNP cord-319626-f1b3ygg0 285 7 and and CC cord-319626-f1b3ygg0 285 8 CCCP CCCP NNP cord-319626-f1b3ygg0 285 9 inhibit inhibit VBP cord-319626-f1b3ygg0 285 10 the the DT cord-319626-f1b3ygg0 285 11 glycosylation glycosylation NN cord-319626-f1b3ygg0 285 12 of of IN cord-319626-f1b3ygg0 285 13 E1 e1 NN cord-319626-f1b3ygg0 285 14 in in IN cord-319626-f1b3ygg0 285 15 a a DT cord-319626-f1b3ygg0 285 16 manner manner NN cord-319626-f1b3ygg0 285 17 essentially essentially RB cord-319626-f1b3ygg0 285 18 identical identical JJ cord-319626-f1b3ygg0 285 19 to to IN cord-319626-f1b3ygg0 285 20 the the DT cord-319626-f1b3ygg0 285 21 inhibition inhibition NN cord-319626-f1b3ygg0 285 22 at at IN cord-319626-f1b3ygg0 285 23 31 31 CD cord-319626-f1b3ygg0 285 24 ° ° NN cord-319626-f1b3ygg0 285 25 C C NNP cord-319626-f1b3ygg0 285 26 ( ( -LRB- cord-319626-f1b3ygg0 285 27 Tooze Tooze NNP cord-319626-f1b3ygg0 285 28 , , , cord-319626-f1b3ygg0 285 29 1987 1987 CD cord-319626-f1b3ygg0 285 30 ) ) -RRB- cord-319626-f1b3ygg0 285 31 . . . cord-319626-f1b3ygg0 286 1 Such such JJ cord-319626-f1b3ygg0 286 2 results result NNS cord-319626-f1b3ygg0 286 3 must must MD cord-319626-f1b3ygg0 286 4 , , , cord-319626-f1b3ygg0 286 5 however however RB cord-319626-f1b3ygg0 286 6 , , , cord-319626-f1b3ygg0 286 7 be be VB cord-319626-f1b3ygg0 286 8 interpreted interpret VBN cord-319626-f1b3ygg0 286 9 with with IN cord-319626-f1b3ygg0 286 10 some some DT cord-319626-f1b3ygg0 286 11 caution caution NN cord-319626-f1b3ygg0 286 12 because because IN cord-319626-f1b3ygg0 286 13 with with IN cord-319626-f1b3ygg0 286 14 these these DT cord-319626-f1b3ygg0 286 15 drugs drug NNS cord-319626-f1b3ygg0 286 16 it -PRON- PRP cord-319626-f1b3ygg0 286 17 is be VBZ cord-319626-f1b3ygg0 286 18 often often RB cord-319626-f1b3ygg0 286 19 impossible impossible JJ cord-319626-f1b3ygg0 286 20 to to TO cord-319626-f1b3ygg0 286 21 distinguish distinguish VB cord-319626-f1b3ygg0 286 22 between between IN cord-319626-f1b3ygg0 286 23 inhibition inhibition NN cord-319626-f1b3ygg0 286 24 of of IN cord-319626-f1b3ygg0 286 25 transport transport NN cord-319626-f1b3ygg0 286 26 and and CC cord-319626-f1b3ygg0 286 27 direct direct JJ cord-319626-f1b3ygg0 286 28 inhibition inhibition NN cord-319626-f1b3ygg0 286 29 of of IN cord-319626-f1b3ygg0 286 30 the the DT cord-319626-f1b3ygg0 286 31 enzymes enzyme NNS cord-319626-f1b3ygg0 286 32 which which WDT cord-319626-f1b3ygg0 286 33 add add VBP cord-319626-f1b3ygg0 286 34 the the DT cord-319626-f1b3ygg0 286 35 sugars sugar NNS cord-319626-f1b3ygg0 286 36 . . . cord-319626-f1b3ygg0 287 1 The the DT cord-319626-f1b3ygg0 287 2 pathway pathway NN cord-319626-f1b3ygg0 287 3 taken take VBN cord-319626-f1b3ygg0 287 4 by by IN cord-319626-f1b3ygg0 287 5 newly newly RB cord-319626-f1b3ygg0 287 6 synthesized synthesize VBN cord-319626-f1b3ygg0 287 7 E1 e1 NN cord-319626-f1b3ygg0 287 8 is be VBZ cord-319626-f1b3ygg0 287 9 complicated complicate VBN cord-319626-f1b3ygg0 287 10 by by IN cord-319626-f1b3ygg0 287 11 the the DT cord-319626-f1b3ygg0 287 12 intracellular intracellular JJ cord-319626-f1b3ygg0 287 13 budding budding NN cord-319626-f1b3ygg0 287 14 of of IN cord-319626-f1b3ygg0 287 15 the the DT cord-319626-f1b3ygg0 287 16 virus virus NN cord-319626-f1b3ygg0 287 17 . . . cord-319626-f1b3ygg0 288 1 At at IN cord-319626-f1b3ygg0 288 2 its -PRON- PRP$ cord-319626-f1b3ygg0 288 3 simplest simple JJS cord-319626-f1b3ygg0 288 4 , , , cord-319626-f1b3ygg0 288 5 one one PRP cord-319626-f1b3ygg0 288 6 can can MD cord-319626-f1b3ygg0 288 7 envisage envisage VB cord-319626-f1b3ygg0 288 8 that that IN cord-319626-f1b3ygg0 288 9 E1 e1 NN cord-319626-f1b3ygg0 288 10 moves move NNS cord-319626-f1b3ygg0 288 11 to to IN cord-319626-f1b3ygg0 288 12 the the DT cord-319626-f1b3ygg0 288 13 budding bud VBG cord-319626-f1b3ygg0 288 14 compartment compartment NN cord-319626-f1b3ygg0 288 15 , , , cord-319626-f1b3ygg0 288 16 nucleocapsids nucleocapsid NNS cord-319626-f1b3ygg0 288 17 bind bind VBP cord-319626-f1b3ygg0 288 18 and and CC cord-319626-f1b3ygg0 288 19 bud bud NN cord-319626-f1b3ygg0 288 20 , , , cord-319626-f1b3ygg0 288 21 and and CC cord-319626-f1b3ygg0 288 22 E1 E1 NNP cord-319626-f1b3ygg0 288 23 then then RB cord-319626-f1b3ygg0 288 24 moves move VBZ cord-319626-f1b3ygg0 288 25 through through IN cord-319626-f1b3ygg0 288 26 the the DT cord-319626-f1b3ygg0 288 27 secretory secretory JJ cord-319626-f1b3ygg0 288 28 pathway pathway NN cord-319626-f1b3ygg0 288 29 as as IN cord-319626-f1b3ygg0 288 30 part part NN cord-319626-f1b3ygg0 288 31 of of IN cord-319626-f1b3ygg0 288 32 the the DT cord-319626-f1b3ygg0 288 33 maturing mature VBG cord-319626-f1b3ygg0 288 34 virion virion NN cord-319626-f1b3ygg0 288 35 . . . cord-319626-f1b3ygg0 289 1 However however RB cord-319626-f1b3ygg0 289 2 , , , cord-319626-f1b3ygg0 289 3 immunoperoxidase immunoperoxidase NN cord-319626-f1b3ygg0 289 4 labeling labeling NN cord-319626-f1b3ygg0 289 5 shows show VBZ cord-319626-f1b3ygg0 289 6 clearly clearly RB cord-319626-f1b3ygg0 289 7 that that IN cord-319626-f1b3ygg0 289 8 E1 e1 NN cord-319626-f1b3ygg0 289 9 in in IN cord-319626-f1b3ygg0 289 10 the the DT cord-319626-f1b3ygg0 289 11 Golgi Golgi NNP cord-319626-f1b3ygg0 289 12 complex complex NN cord-319626-f1b3ygg0 289 13 is be VBZ cord-319626-f1b3ygg0 289 14 not not RB cord-319626-f1b3ygg0 289 15 only only RB cord-319626-f1b3ygg0 289 16 present present JJ cord-319626-f1b3ygg0 289 17 in in IN cord-319626-f1b3ygg0 289 18 virions virion NNS cord-319626-f1b3ygg0 289 19 but but CC cord-319626-f1b3ygg0 289 20 also also RB cord-319626-f1b3ygg0 289 21 in in IN cord-319626-f1b3ygg0 289 22 every every DT cord-319626-f1b3ygg0 289 23 cisternal cisternal JJ cord-319626-f1b3ygg0 289 24 membrane membrane NN cord-319626-f1b3ygg0 289 25 ( ( -LRB- cord-319626-f1b3ygg0 289 26 see see VB cord-319626-f1b3ygg0 289 27 Fig Fig NNP cord-319626-f1b3ygg0 289 28 . . . cord-319626-f1b3ygg0 289 29 11 11 CD cord-319626-f1b3ygg0 289 30 ) ) -RRB- cord-319626-f1b3ygg0 289 31 . . . cord-319626-f1b3ygg0 290 1 The the DT cord-319626-f1b3ygg0 290 2 amount amount NN cord-319626-f1b3ygg0 290 3 of of IN cord-319626-f1b3ygg0 290 4 E1 e1 NN cord-319626-f1b3ygg0 290 5 in in IN cord-319626-f1b3ygg0 290 6 Golgi Golgi NNP cord-319626-f1b3ygg0 290 7 membranes membrane NNS cord-319626-f1b3ygg0 290 8 can can MD cord-319626-f1b3ygg0 290 9 not not RB cord-319626-f1b3ygg0 290 10 be be VB cord-319626-f1b3ygg0 290 11 estimated estimate VBN cord-319626-f1b3ygg0 290 12 because because IN cord-319626-f1b3ygg0 290 13 of of IN cord-319626-f1b3ygg0 290 14 the the DT cord-319626-f1b3ygg0 290 15 catalytic catalytic JJ cord-319626-f1b3ygg0 290 16 nature nature NN cord-319626-f1b3ygg0 290 17 of of IN cord-319626-f1b3ygg0 290 18 the the DT cord-319626-f1b3ygg0 290 19 peroxidase peroxidase NN cord-319626-f1b3ygg0 290 20 technique technique NN cord-319626-f1b3ygg0 290 21 but but CC cord-319626-f1b3ygg0 290 22 , , , cord-319626-f1b3ygg0 290 23 until until IN cord-319626-f1b3ygg0 290 24 very very RB cord-319626-f1b3ygg0 290 25 late late JJ cord-319626-f1b3ygg0 290 26 in in IN cord-319626-f1b3ygg0 290 27 the the DT cord-319626-f1b3ygg0 290 28 viral viral JJ cord-319626-f1b3ygg0 290 29 infection infection NN cord-319626-f1b3ygg0 290 30 , , , cord-319626-f1b3ygg0 290 31 it -PRON- PRP cord-319626-f1b3ygg0 290 32 must must MD cord-319626-f1b3ygg0 290 33 be be VB cord-319626-f1b3ygg0 290 34 lower low JJR cord-319626-f1b3ygg0 290 35 than than IN cord-319626-f1b3ygg0 290 36 that that DT cord-319626-f1b3ygg0 290 37 needed need VBN cord-319626-f1b3ygg0 290 38 to to TO cord-319626-f1b3ygg0 290 39 support support VB cord-319626-f1b3ygg0 290 40 budding budding NN cord-319626-f1b3ygg0 290 41 of of IN cord-319626-f1b3ygg0 290 42 the the DT cord-319626-f1b3ygg0 290 43 virus virus NN cord-319626-f1b3ygg0 290 44 . . . cord-319626-f1b3ygg0 291 1 This this DT cord-319626-f1b3ygg0 291 2 accumulation accumulation NN cord-319626-f1b3ygg0 291 3 of of IN cord-319626-f1b3ygg0 291 4 E1 e1 NN cord-319626-f1b3ygg0 291 5 in in IN cord-319626-f1b3ygg0 291 6 the the DT cord-319626-f1b3ygg0 291 7 Golgi Golgi NNP cord-319626-f1b3ygg0 291 8 membranes membrane NNS cord-319626-f1b3ygg0 291 9 during during IN cord-319626-f1b3ygg0 291 10 infection infection NN cord-319626-f1b3ygg0 291 11 may may MD cord-319626-f1b3ygg0 291 12 represent represent VB cord-319626-f1b3ygg0 291 13 a a DT cord-319626-f1b3ygg0 291 14 dead dead JJ cord-319626-f1b3ygg0 291 15 end end NN cord-319626-f1b3ygg0 291 16 caused cause VBN cord-319626-f1b3ygg0 291 17 by by IN cord-319626-f1b3ygg0 291 18 " " `` cord-319626-f1b3ygg0 291 19 leakage leakage NN cord-319626-f1b3ygg0 291 20 " " '' cord-319626-f1b3ygg0 291 21 of of IN cord-319626-f1b3ygg0 291 22 E1 e1 NN cord-319626-f1b3ygg0 291 23 from from IN cord-319626-f1b3ygg0 291 24 the the DT cord-319626-f1b3ygg0 291 25 budding bud VBG cord-319626-f1b3ygg0 291 26 compartment compartment NN cord-319626-f1b3ygg0 291 27 if if IN cord-319626-f1b3ygg0 291 28 the the DT cord-319626-f1b3ygg0 291 29 nucleocapsids nucleocapsid NNS cord-319626-f1b3ygg0 291 30 are be VBP cord-319626-f1b3ygg0 291 31 unable unable JJ cord-319626-f1b3ygg0 291 32 to to TO cord-319626-f1b3ygg0 291 33 trap trap VB cord-319626-f1b3ygg0 291 34 all all DT cord-319626-f1b3ygg0 291 35 of of IN cord-319626-f1b3ygg0 291 36 the the DT cord-319626-f1b3ygg0 291 37 E1 e1 NN cord-319626-f1b3ygg0 291 38 in in IN cord-319626-f1b3ygg0 291 39 budding bud VBG cord-319626-f1b3ygg0 291 40 virions virion NNS cord-319626-f1b3ygg0 291 41 . . . cord-319626-f1b3ygg0 292 1 This this DT cord-319626-f1b3ygg0 292 2 idea idea NN cord-319626-f1b3ygg0 292 3 is be VBZ cord-319626-f1b3ygg0 292 4 supported support VBN cord-319626-f1b3ygg0 292 5 by by IN cord-319626-f1b3ygg0 292 6 the the DT cord-319626-f1b3ygg0 292 7 finding finding NN cord-319626-f1b3ygg0 292 8 that that IN cord-319626-f1b3ygg0 292 9 E1 e1 NN cord-319626-f1b3ygg0 292 10 is be VBZ cord-319626-f1b3ygg0 292 11 not not RB cord-319626-f1b3ygg0 292 12 transported transport VBN cord-319626-f1b3ygg0 292 13 beyond beyond IN cord-319626-f1b3ygg0 292 14 the the DT cord-319626-f1b3ygg0 292 15 Golgi Golgi NNP cord-319626-f1b3ygg0 292 16 stack stack NN cord-319626-f1b3ygg0 292 17 in in IN cord-319626-f1b3ygg0 292 18 cellular cellular JJ cord-319626-f1b3ygg0 292 19 membranes membrane NNS cord-319626-f1b3ygg0 292 20 ( ( -LRB- cord-319626-f1b3ygg0 292 21 Tooze Tooze NNP cord-319626-f1b3ygg0 292 22 and and CC cord-319626-f1b3ygg0 292 23 Stanley Stanley NNP cord-319626-f1b3ygg0 292 24 , , , cord-319626-f1b3ygg0 292 25 1986 1986 CD cord-319626-f1b3ygg0 292 26 ; ; : cord-319626-f1b3ygg0 292 27 Tooze Tooze NNP cord-319626-f1b3ygg0 292 28 et et NNP cord-319626-f1b3ygg0 292 29 al al NNP cord-319626-f1b3ygg0 292 30 . . NNP cord-319626-f1b3ygg0 292 31 , , , cord-319626-f1b3ygg0 292 32 1987 1987 CD cord-319626-f1b3ygg0 292 33 ) ) -RRB- cord-319626-f1b3ygg0 292 34 . . . cord-319626-f1b3ygg0 293 1 Alternatively alternatively RB cord-319626-f1b3ygg0 293 2 , , , cord-319626-f1b3ygg0 293 3 cisternal cisternal JJ cord-319626-f1b3ygg0 293 4 E1 E1 NNP cord-319626-f1b3ygg0 293 5 may may MD cord-319626-f1b3ygg0 293 6 represent represent VB cord-319626-f1b3ygg0 293 7 a a DT cord-319626-f1b3ygg0 293 8 genuine genuine JJ cord-319626-f1b3ygg0 293 9 part part NN cord-319626-f1b3ygg0 293 10 of of IN cord-319626-f1b3ygg0 293 11 the the DT cord-319626-f1b3ygg0 293 12 maturation maturation NN cord-319626-f1b3ygg0 293 13 pathway pathway NN cord-319626-f1b3ygg0 293 14 . . . cord-319626-f1b3ygg0 294 1 El El NNP cord-319626-f1b3ygg0 294 2 may may MD cord-319626-f1b3ygg0 294 3 have have VB cord-319626-f1b3ygg0 294 4 to to TO cord-319626-f1b3ygg0 294 5 move move VB cord-319626-f1b3ygg0 294 6 through through IN cord-319626-f1b3ygg0 294 7 Golgi Golgi NNP cord-319626-f1b3ygg0 294 8 cisternae cisternae NNP cord-319626-f1b3ygg0 294 9 and and CC cord-319626-f1b3ygg0 294 10 then then RB cord-319626-f1b3ygg0 294 11 back back RB cord-319626-f1b3ygg0 294 12 again again RB cord-319626-f1b3ygg0 294 13 to to IN cord-319626-f1b3ygg0 294 14 the the DT cord-319626-f1b3ygg0 294 15 budding bud VBG cord-319626-f1b3ygg0 294 16 compartment compartment NN cord-319626-f1b3ygg0 294 17 before before IN cord-319626-f1b3ygg0 294 18 it -PRON- PRP cord-319626-f1b3ygg0 294 19 can can MD cord-319626-f1b3ygg0 294 20 participate participate VB cord-319626-f1b3ygg0 294 21 in in IN cord-319626-f1b3ygg0 294 22 the the DT cord-319626-f1b3ygg0 294 23 budding budding JJ cord-319626-f1b3ygg0 294 24 reaction reaction NN cord-319626-f1b3ygg0 294 25 . . . cord-319626-f1b3ygg0 295 1 This this DT cord-319626-f1b3ygg0 295 2 seems seem VBZ cord-319626-f1b3ygg0 295 3 very very RB cord-319626-f1b3ygg0 295 4 unlikely unlikely JJ cord-319626-f1b3ygg0 295 5 , , , cord-319626-f1b3ygg0 295 6 however however RB cord-319626-f1b3ygg0 295 7 , , , cord-319626-f1b3ygg0 295 8 because because IN cord-319626-f1b3ygg0 295 9 all all DT cord-319626-f1b3ygg0 295 10 attempts attempt NNS cord-319626-f1b3ygg0 295 11 to to TO cord-319626-f1b3ygg0 295 12 show show VB cord-319626-f1b3ygg0 295 13 movement movement NN cord-319626-f1b3ygg0 295 14 of of IN cord-319626-f1b3ygg0 295 15 other other JJ cord-319626-f1b3ygg0 295 16 ER ER NNP cord-319626-f1b3ygg0 295 17 proteins protein NNS cord-319626-f1b3ygg0 295 18 to to IN cord-319626-f1b3ygg0 295 19 the the DT cord-319626-f1b3ygg0 295 20 Golgi Golgi NNP cord-319626-f1b3ygg0 295 21 stack stack NN cord-319626-f1b3ygg0 295 22 and and CC cord-319626-f1b3ygg0 295 23 back back NN cord-319626-f1b3ygg0 295 24 again again RB cord-319626-f1b3ygg0 295 25 have have VBP cord-319626-f1b3ygg0 295 26 failed fail VBN cord-319626-f1b3ygg0 295 27 ( ( -LRB- cord-319626-f1b3ygg0 295 28 Yamamoto Yamamoto NNP cord-319626-f1b3ygg0 295 29 et et NNP cord-319626-f1b3ygg0 295 30 al al NNP cord-319626-f1b3ygg0 295 31 . . NNP cord-319626-f1b3ygg0 295 32 , , , cord-319626-f1b3ygg0 295 33 1985 1985 CD cord-319626-f1b3ygg0 295 34 ; ; : cord-319626-f1b3ygg0 295 35 Brands Brands NNPS cord-319626-f1b3ygg0 295 36 et et NNP cord-319626-f1b3ygg0 295 37 al al NNP cord-319626-f1b3ygg0 295 38 . . NNP cord-319626-f1b3ygg0 295 39 , , , cord-319626-f1b3ygg0 295 40 1985 1985 CD cord-319626-f1b3ygg0 295 41 ) ) -RRB- cord-319626-f1b3ygg0 295 42 . . . cord-319626-f1b3ygg0 296 1 It -PRON- PRP cord-319626-f1b3ygg0 296 2 is be VBZ cord-319626-f1b3ygg0 296 3 , , , cord-319626-f1b3ygg0 296 4 therefore therefore RB cord-319626-f1b3ygg0 296 5 , , , cord-319626-f1b3ygg0 296 6 more more RBR cord-319626-f1b3ygg0 296 7 reasonable reasonable JJ cord-319626-f1b3ygg0 296 8 to to TO cord-319626-f1b3ygg0 296 9 assume assume VB cord-319626-f1b3ygg0 296 10 that that IN cord-319626-f1b3ygg0 296 11 E1 E1 NNP cord-319626-f1b3ygg0 296 12 is be VBZ cord-319626-f1b3ygg0 296 13 transported transport VBN cord-319626-f1b3ygg0 296 14 from from IN cord-319626-f1b3ygg0 296 15 the the DT cord-319626-f1b3ygg0 296 16 membrane membrane NN cord-319626-f1b3ygg0 296 17 of of IN cord-319626-f1b3ygg0 296 18 the the DT cord-319626-f1b3ygg0 296 19 ER ER NNP cord-319626-f1b3ygg0 296 20 to to IN cord-319626-f1b3ygg0 296 21 the the DT cord-319626-f1b3ygg0 296 22 budding bud VBG cord-319626-f1b3ygg0 296 23 compartment compartment NN cord-319626-f1b3ygg0 296 24 where where WRB cord-319626-f1b3ygg0 296 25 it -PRON- PRP cord-319626-f1b3ygg0 296 26 reaches reach VBZ cord-319626-f1b3ygg0 296 27 a a DT cord-319626-f1b3ygg0 296 28 concentration concentration NN cord-319626-f1b3ygg0 296 29 sufficient sufficient JJ cord-319626-f1b3ygg0 296 30 to to TO cord-319626-f1b3ygg0 296 31 allow allow VB cord-319626-f1b3ygg0 296 32 budding bud VBG cord-319626-f1b3ygg0 296 33 for for IN cord-319626-f1b3ygg0 296 34 the the DT cord-319626-f1b3ygg0 296 35 first first JJ cord-319626-f1b3ygg0 296 36 time time NN cord-319626-f1b3ygg0 296 37 . . . cord-319626-f1b3ygg0 297 1 Thereafter thereafter RB cord-319626-f1b3ygg0 297 2 , , , cord-319626-f1b3ygg0 297 3 incorporated incorporate VBN cord-319626-f1b3ygg0 297 4 into into IN cord-319626-f1b3ygg0 297 5 the the DT cord-319626-f1b3ygg0 297 6 envelope envelope NN cord-319626-f1b3ygg0 297 7 of of IN cord-319626-f1b3ygg0 297 8 progeny progeny JJ cord-319626-f1b3ygg0 297 9 virions virion NNS cord-319626-f1b3ygg0 297 10 , , , cord-319626-f1b3ygg0 297 11 it -PRON- PRP cord-319626-f1b3ygg0 297 12 moves move VBZ cord-319626-f1b3ygg0 297 13 through through IN cord-319626-f1b3ygg0 297 14 the the DT cord-319626-f1b3ygg0 297 15 Golgi Golgi NNP cord-319626-f1b3ygg0 297 16 stack stack NN cord-319626-f1b3ygg0 297 17 and and CC cord-319626-f1b3ygg0 297 18 thence thence RB cord-319626-f1b3ygg0 297 19 to to IN cord-319626-f1b3ygg0 297 20 the the DT cord-319626-f1b3ygg0 297 21 cell cell NN cord-319626-f1b3ygg0 297 22 surface surface NN cord-319626-f1b3ygg0 297 23 with with IN cord-319626-f1b3ygg0 297 24 only only RB cord-319626-f1b3ygg0 297 25 a a DT cord-319626-f1b3ygg0 297 26 small small JJ cord-319626-f1b3ygg0 297 27 amount amount NN cord-319626-f1b3ygg0 297 28 leaking leak VBG cord-319626-f1b3ygg0 297 29 to to IN cord-319626-f1b3ygg0 297 30 the the DT cord-319626-f1b3ygg0 297 31 Golgi Golgi NNP cord-319626-f1b3ygg0 297 32 stack stack NN cord-319626-f1b3ygg0 297 33 independent independent JJ cord-319626-f1b3ygg0 297 34 of of IN cord-319626-f1b3ygg0 297 35 the the DT cord-319626-f1b3ygg0 297 36 virions virion NNS cord-319626-f1b3ygg0 297 37 . . . cord-319626-f1b3ygg0 298 1 As as IN cord-319626-f1b3ygg0 298 2 the the DT cord-319626-f1b3ygg0 298 3 infection infection NN cord-319626-f1b3ygg0 298 4 progresses progress VBZ cord-319626-f1b3ygg0 298 5 the the DT cord-319626-f1b3ygg0 298 6 amount amount NN cord-319626-f1b3ygg0 298 7 of of IN cord-319626-f1b3ygg0 298 8 El El NNP cord-319626-f1b3ygg0 298 9 being be VBG cord-319626-f1b3ygg0 298 10 synthesized synthesize VBN cord-319626-f1b3ygg0 298 11 increases increase NNS cord-319626-f1b3ygg0 298 12 ( ( -LRB- cord-319626-f1b3ygg0 298 13 our -PRON- PRP$ cord-319626-f1b3ygg0 298 14 unpublished unpublished JJ cord-319626-f1b3ygg0 298 15 data datum NNS cord-319626-f1b3ygg0 298 16 ) ) -RRB- cord-319626-f1b3ygg0 298 17 and and CC cord-319626-f1b3ygg0 298 18 at at IN cord-319626-f1b3ygg0 298 19 the the DT cord-319626-f1b3ygg0 298 20 same same JJ cord-319626-f1b3ygg0 298 21 time time NN cord-319626-f1b3ygg0 298 22 budding bud VBG cord-319626-f1b3ygg0 298 23 of of IN cord-319626-f1b3ygg0 298 24 progeny progeny JJ cord-319626-f1b3ygg0 298 25 virions virion NNS cord-319626-f1b3ygg0 298 26 directly directly RB cord-319626-f1b3ygg0 298 27 into into IN cord-319626-f1b3ygg0 298 28 the the DT cord-319626-f1b3ygg0 298 29 lumen lumen NN cord-319626-f1b3ygg0 298 30 of of IN cord-319626-f1b3ygg0 298 31 the the DT cord-319626-f1b3ygg0 298 32 rough rough JJ cord-319626-f1b3ygg0 298 33 ER er NN cord-319626-f1b3ygg0 298 34 begins begin VBZ cord-319626-f1b3ygg0 298 35 and and CC cord-319626-f1b3ygg0 298 36 increases increase NNS cord-319626-f1b3ygg0 298 37 . . . cord-319626-f1b3ygg0 299 1 We -PRON- PRP cord-319626-f1b3ygg0 299 2 interpret interpret VBP cord-319626-f1b3ygg0 299 3 this this DT cord-319626-f1b3ygg0 299 4 to to TO cord-319626-f1b3ygg0 299 5 indicate indicate VB cord-319626-f1b3ygg0 299 6 that that IN cord-319626-f1b3ygg0 299 7 the the DT cord-319626-f1b3ygg0 299 8 rate rate NN cord-319626-f1b3ygg0 299 9 of of IN cord-319626-f1b3ygg0 299 10 insertion insertion NN cord-319626-f1b3ygg0 299 11 of of IN cord-319626-f1b3ygg0 299 12 E1 e1 NN cord-319626-f1b3ygg0 299 13 into into IN cord-319626-f1b3ygg0 299 14 the the DT cord-319626-f1b3ygg0 299 15 rough rough JJ cord-319626-f1b3ygg0 299 16 ER er NN cord-319626-f1b3ygg0 299 17 membrane membrane NN cord-319626-f1b3ygg0 299 18 beings being NNS cord-319626-f1b3ygg0 299 19 to to TO cord-319626-f1b3ygg0 299 20 exceed exceed VB cord-319626-f1b3ygg0 299 21 the the DT cord-319626-f1b3ygg0 299 22 rate rate NN cord-319626-f1b3ygg0 299 23 of of IN cord-319626-f1b3ygg0 299 24 its -PRON- PRP$ cord-319626-f1b3ygg0 299 25 transport transport NN cord-319626-f1b3ygg0 299 26 out out IN cord-319626-f1b3ygg0 299 27 of of IN cord-319626-f1b3ygg0 299 28 the the DT cord-319626-f1b3ygg0 299 29 ER ER NNP cord-319626-f1b3ygg0 299 30 to to IN cord-319626-f1b3ygg0 299 31 the the DT cord-319626-f1b3ygg0 299 32 budding bud VBG cord-319626-f1b3ygg0 299 33 compartment compartment NN cord-319626-f1b3ygg0 299 34 . . . cord-319626-f1b3ygg0 300 1 Reducing reduce VBG cord-319626-f1b3ygg0 300 2 the the DT cord-319626-f1b3ygg0 300 3 temperature temperature NN cord-319626-f1b3ygg0 300 4 to to IN cord-319626-f1b3ygg0 300 5 31 31 CD cord-319626-f1b3ygg0 300 6 ° ° NN cord-319626-f1b3ygg0 300 7 C C NNP cord-319626-f1b3ygg0 300 8 profoundly profoundly RB cord-319626-f1b3ygg0 300 9 inhibits inhibit VBZ cord-319626-f1b3ygg0 300 10 the the DT cord-319626-f1b3ygg0 300 11 release release NN cord-319626-f1b3ygg0 300 12 of of IN cord-319626-f1b3ygg0 300 13 virions virion NNS cord-319626-f1b3ygg0 300 14 into into IN cord-319626-f1b3ygg0 300 15 the the DT cord-319626-f1b3ygg0 300 16 medium medium NN cord-319626-f1b3ygg0 300 17 . . . cord-319626-f1b3ygg0 301 1 All all PDT cord-319626-f1b3ygg0 301 2 the the DT cord-319626-f1b3ygg0 301 3 morphological morphological JJ cord-319626-f1b3ygg0 301 4 evidence evidence NN cord-319626-f1b3ygg0 301 5 suggests suggest VBZ cord-319626-f1b3ygg0 301 6 that that IN cord-319626-f1b3ygg0 301 7 at at IN cord-319626-f1b3ygg0 301 8 31 31 CD cord-319626-f1b3ygg0 301 9 ° ° NN cord-319626-f1b3ygg0 301 10 C c NN cord-319626-f1b3ygg0 301 11 it -PRON- PRP cord-319626-f1b3ygg0 301 12 is be VBZ cord-319626-f1b3ygg0 301 13 the the DT cord-319626-f1b3ygg0 301 14 entry entry NN cord-319626-f1b3ygg0 301 15 of of IN cord-319626-f1b3ygg0 301 16 virions virion NNS cord-319626-f1b3ygg0 301 17 into into IN cord-319626-f1b3ygg0 301 18 the the DT cord-319626-f1b3ygg0 301 19 Golgi Golgi NNP cord-319626-f1b3ygg0 301 20 complex complex NN cord-319626-f1b3ygg0 301 21 that that WDT cord-319626-f1b3ygg0 301 22 is be VBZ cord-319626-f1b3ygg0 301 23 inhibited inhibit VBN cord-319626-f1b3ygg0 301 24 ; ; : cord-319626-f1b3ygg0 301 25 as as IN cord-319626-f1b3ygg0 301 26 a a DT cord-319626-f1b3ygg0 301 27 result result NN cord-319626-f1b3ygg0 301 28 , , , cord-319626-f1b3ygg0 301 29 few few JJ cord-319626-f1b3ygg0 301 30 virions virion NNS cord-319626-f1b3ygg0 301 31 are be VBP cord-319626-f1b3ygg0 301 32 seen see VBN cord-319626-f1b3ygg0 301 33 in in IN cord-319626-f1b3ygg0 301 34 the the DT cord-319626-f1b3ygg0 301 35 Golgi Golgi NNP cord-319626-f1b3ygg0 301 36 cisternae cisternae NNP cord-319626-f1b3ygg0 301 37 or or CC cord-319626-f1b3ygg0 301 38 in in IN cord-319626-f1b3ygg0 301 39 the the DT cord-319626-f1b3ygg0 301 40 post post JJ cord-319626-f1b3ygg0 301 41 Golgi Golgi NNP cord-319626-f1b3ygg0 301 42 transport transport NN cord-319626-f1b3ygg0 301 43 vesicles vesicle NNS cord-319626-f1b3ygg0 301 44 and and CC cord-319626-f1b3ygg0 301 45 the the DT cord-319626-f1b3ygg0 301 46 Golgi Golgi NNP cord-319626-f1b3ygg0 301 47 stack stack NN cord-319626-f1b3ygg0 301 48 maintains maintain VBZ cord-319626-f1b3ygg0 301 49 its -PRON- PRP$ cord-319626-f1b3ygg0 301 50 normal normal JJ cord-319626-f1b3ygg0 301 51 morphology morphology NN cord-319626-f1b3ygg0 301 52 . . . cord-319626-f1b3ygg0 302 1 The the DT cord-319626-f1b3ygg0 302 2 inhibition inhibition NN cord-319626-f1b3ygg0 302 3 of of IN cord-319626-f1b3ygg0 302 4 virion virion NN cord-319626-f1b3ygg0 302 5 transport transport NN cord-319626-f1b3ygg0 302 6 is be VBZ cord-319626-f1b3ygg0 302 7 accompanied accompany VBN cord-319626-f1b3ygg0 302 8 by by IN cord-319626-f1b3ygg0 302 9 an an DT cord-319626-f1b3ygg0 302 10 inhibition inhibition NN cord-319626-f1b3ygg0 302 11 of of IN cord-319626-f1b3ygg0 302 12 terminal terminal JJ cord-319626-f1b3ygg0 302 13 glycosylation glycosylation NN cord-319626-f1b3ygg0 302 14 . . . cord-319626-f1b3ygg0 303 1 This this DT cord-319626-f1b3ygg0 303 2 would would MD cord-319626-f1b3ygg0 303 3 be be VB cord-319626-f1b3ygg0 303 4 expected expect VBN cord-319626-f1b3ygg0 303 5 if if IN cord-319626-f1b3ygg0 303 6 ( ( -LRB- cord-319626-f1b3ygg0 303 7 a a DT cord-319626-f1b3ygg0 303 8 ) ) -RRB- cord-319626-f1b3ygg0 303 9 terminal terminal JJ cord-319626-f1b3ygg0 303 10 glycosylation glycosylation NN cord-319626-f1b3ygg0 303 11 occurred occur VBD cord-319626-f1b3ygg0 303 12 in in IN cord-319626-f1b3ygg0 303 13 the the DT cord-319626-f1b3ygg0 303 14 trans trans NNP cord-319626-f1b3ygg0 303 15 - - NNP cord-319626-f1b3ygg0 303 16 Golgi Golgi NNP cord-319626-f1b3ygg0 303 17 complex complex NN cord-319626-f1b3ygg0 303 18 and and CC cord-319626-f1b3ygg0 303 19 , , , cord-319626-f1b3ygg0 303 20 ( ( -LRB- cord-319626-f1b3ygg0 303 21 b b LS cord-319626-f1b3ygg0 303 22 ) ) -RRB- cord-319626-f1b3ygg0 303 23 if if IN cord-319626-f1b3ygg0 303 24 the the DT cord-319626-f1b3ygg0 303 25 E1 E1 NNP cord-319626-f1b3ygg0 303 26 is be VBZ cord-319626-f1b3ygg0 303 27 delivered deliver VBN cord-319626-f1b3ygg0 303 28 there there RB cord-319626-f1b3ygg0 303 29 normally normally RB cord-319626-f1b3ygg0 303 30 incorporated incorporate VBN cord-319626-f1b3ygg0 303 31 in in IN cord-319626-f1b3ygg0 303 32 the the DT cord-319626-f1b3ygg0 303 33 envelopes envelope NNS cord-319626-f1b3ygg0 303 34 of of IN cord-319626-f1b3ygg0 303 35 progeny progeny JJ cord-319626-f1b3ygg0 303 36 virions virion NNS cord-319626-f1b3ygg0 303 37 rather rather RB cord-319626-f1b3ygg0 303 38 than than IN cord-319626-f1b3ygg0 303 39 as as IN cord-319626-f1b3ygg0 303 40 an an DT cord-319626-f1b3ygg0 303 41 integral integral JJ cord-319626-f1b3ygg0 303 42 protein protein NN cord-319626-f1b3ygg0 303 43 of of IN cord-319626-f1b3ygg0 303 44 cellular cellular JJ cord-319626-f1b3ygg0 303 45 membranes membrane NNS cord-319626-f1b3ygg0 303 46 . . . cord-319626-f1b3ygg0 304 1 But but CC cord-319626-f1b3ygg0 304 2 at at IN cord-319626-f1b3ygg0 304 3 31 31 CD cord-319626-f1b3ygg0 304 4 ° ° NN cord-319626-f1b3ygg0 304 5 C c NN cord-319626-f1b3ygg0 304 6 , , , cord-319626-f1b3ygg0 304 7 addition addition NN cord-319626-f1b3ygg0 304 8 of of IN cord-319626-f1b3ygg0 304 9 GalNAc GalNAc NNP cord-319626-f1b3ygg0 304 10 still still RB cord-319626-f1b3ygg0 304 11 occurs occur VBZ cord-319626-f1b3ygg0 304 12 to to IN cord-319626-f1b3ygg0 304 13 an an DT cord-319626-f1b3ygg0 304 14 unchanged unchanged JJ cord-319626-f1b3ygg0 304 15 extent extent NN cord-319626-f1b3ygg0 304 16 suggesting suggest VBG cord-319626-f1b3ygg0 304 17 that that IN cord-319626-f1b3ygg0 304 18 this this DT cord-319626-f1b3ygg0 304 19 sugar sugar NN cord-319626-f1b3ygg0 304 20 is be VBZ cord-319626-f1b3ygg0 304 21 added add VBN cord-319626-f1b3ygg0 304 22 in in IN cord-319626-f1b3ygg0 304 23 or or CC cord-319626-f1b3ygg0 304 24 just just RB cord-319626-f1b3ygg0 304 25 before before IN cord-319626-f1b3ygg0 304 26 the the DT cord-319626-f1b3ygg0 304 27 budding bud VBG cord-319626-f1b3ygg0 304 28 compartment compartment NN cord-319626-f1b3ygg0 304 29 . . . cord-319626-f1b3ygg0 305 1 Interestingly interestingly RB cord-319626-f1b3ygg0 305 2 , , , cord-319626-f1b3ygg0 305 3 the the DT cord-319626-f1b3ygg0 305 4 amount amount NN cord-319626-f1b3ygg0 305 5 of of IN cord-319626-f1b3ygg0 305 6 E1 e1 NN cord-319626-f1b3ygg0 305 7 containing contain VBG cord-319626-f1b3ygg0 305 8 GalNAc GalNAc NNP cord-319626-f1b3ygg0 305 9 ( ( -LRB- cord-319626-f1b3ygg0 305 10 El0 el0 NN cord-319626-f1b3ygg0 305 11 does do VBZ cord-319626-f1b3ygg0 305 12 not not RB cord-319626-f1b3ygg0 305 13 change change VB cord-319626-f1b3ygg0 305 14 when when WRB cord-319626-f1b3ygg0 305 15 the the DT cord-319626-f1b3ygg0 305 16 temperature temperature NN cord-319626-f1b3ygg0 305 17 is be VBZ cord-319626-f1b3ygg0 305 18 reduced reduce VBN cord-319626-f1b3ygg0 305 19 . . . cord-319626-f1b3ygg0 306 1 The the DT cord-319626-f1b3ygg0 306 2 de de NN cord-319626-f1b3ygg0 306 3 - - NN cord-319626-f1b3ygg0 306 4 crease crease NN cord-319626-f1b3ygg0 306 5 in in IN cord-319626-f1b3ygg0 306 6 Elm Elm NNP cord-319626-f1b3ygg0 306 7 is be VBZ cord-319626-f1b3ygg0 306 8 accompanied accompany VBN cord-319626-f1b3ygg0 306 9 only only RB cord-319626-f1b3ygg0 306 10 by by IN cord-319626-f1b3ygg0 306 11 an an DT cord-319626-f1b3ygg0 306 12 increase increase NN cord-319626-f1b3ygg0 306 13 in in IN cord-319626-f1b3ygg0 306 14 El El NNP cord-319626-f1b3ygg0 306 15 ° ° NN cord-319626-f1b3ygg0 306 16 . . . cord-319626-f1b3ygg0 307 1 This this DT cord-319626-f1b3ygg0 307 2 correlates correlate VBZ cord-319626-f1b3ygg0 307 3 with with IN cord-319626-f1b3ygg0 307 4 the the DT cord-319626-f1b3ygg0 307 5 increase increase NN cord-319626-f1b3ygg0 307 6 in in IN cord-319626-f1b3ygg0 307 7 budding bud VBG cord-319626-f1b3ygg0 307 8 into into IN cord-319626-f1b3ygg0 307 9 the the DT cord-319626-f1b3ygg0 307 10 rough rough JJ cord-319626-f1b3ygg0 307 11 ER er NN cord-319626-f1b3ygg0 307 12 and and CC cord-319626-f1b3ygg0 307 13 further further RB cord-319626-f1b3ygg0 307 14 argues argue VBZ cord-319626-f1b3ygg0 307 15 that that IN cord-319626-f1b3ygg0 307 16 GalNac GalNac NNP cord-319626-f1b3ygg0 307 17 is be VBZ cord-319626-f1b3ygg0 307 18 not not RB cord-319626-f1b3ygg0 307 19 added add VBN cord-319626-f1b3ygg0 307 20 in in IN cord-319626-f1b3ygg0 307 21 the the DT cord-319626-f1b3ygg0 307 22 rough rough JJ cord-319626-f1b3ygg0 307 23 ER er NN cord-319626-f1b3ygg0 307 24 but but CC cord-319626-f1b3ygg0 307 25 in in IN cord-319626-f1b3ygg0 307 26 the the DT cord-319626-f1b3ygg0 307 27 budding bud VBG cord-319626-f1b3ygg0 307 28 compartment compartment NN cord-319626-f1b3ygg0 307 29 . . . cord-319626-f1b3ygg0 308 1 If if IN cord-319626-f1b3ygg0 308 2 GalNAc GalNAc NNP cord-319626-f1b3ygg0 308 3 was be VBD cord-319626-f1b3ygg0 308 4 added add VBN cord-319626-f1b3ygg0 308 5 either either CC cord-319626-f1b3ygg0 308 6 cotranslationally cotranslationally NN cord-319626-f1b3ygg0 308 7 or or CC cord-319626-f1b3ygg0 308 8 while while IN cord-319626-f1b3ygg0 308 9 the the DT cord-319626-f1b3ygg0 308 10 E1 e1 NN cord-319626-f1b3ygg0 308 11 resided reside VBD cord-319626-f1b3ygg0 308 12 in in IN cord-319626-f1b3ygg0 308 13 the the DT cord-319626-f1b3ygg0 308 14 rough rough JJ cord-319626-f1b3ygg0 308 15 ER er NN cord-319626-f1b3ygg0 308 16 membrane membrane NN cord-319626-f1b3ygg0 308 17 , , , cord-319626-f1b3ygg0 308 18 an an DT cord-319626-f1b3ygg0 308 19 increase increase NN cord-319626-f1b3ygg0 308 20 in in IN cord-319626-f1b3ygg0 308 21 the the DT cord-319626-f1b3ygg0 308 22 amount amount NN cord-319626-f1b3ygg0 308 23 of of IN cord-319626-f1b3ygg0 308 24 the the DT cord-319626-f1b3ygg0 308 25 Eli Eli NNP cord-319626-f1b3ygg0 308 26 form form NN cord-319626-f1b3ygg0 308 27 would would MD cord-319626-f1b3ygg0 308 28 be be VB cord-319626-f1b3ygg0 308 29 expected expect VBN cord-319626-f1b3ygg0 308 30 both both CC cord-319626-f1b3ygg0 308 31 at at IN cord-319626-f1b3ygg0 308 32 37 37 CD cord-319626-f1b3ygg0 308 33 and and CC cord-319626-f1b3ygg0 308 34 31 31 CD cord-319626-f1b3ygg0 308 35 ° ° NN cord-319626-f1b3ygg0 308 36 C c NN cord-319626-f1b3ygg0 308 37 as as IN cord-319626-f1b3ygg0 308 38 the the DT cord-319626-f1b3ygg0 308 39 concentration concentration NN cord-319626-f1b3ygg0 308 40 of of IN cord-319626-f1b3ygg0 308 41 E1 e1 NN cord-319626-f1b3ygg0 308 42 reaches reach VBZ cord-319626-f1b3ygg0 308 43 levels level NNS cord-319626-f1b3ygg0 308 44 that that IN cord-319626-f1b3ygg0 308 45 not not RB cord-319626-f1b3ygg0 308 46 only only RB cord-319626-f1b3ygg0 308 47 support support VBP cord-319626-f1b3ygg0 308 48 budding bud VBG cord-319626-f1b3ygg0 308 49 into into IN cord-319626-f1b3ygg0 308 50 this this DT cord-319626-f1b3ygg0 308 51 compartment compartment NN cord-319626-f1b3ygg0 308 52 but but CC cord-319626-f1b3ygg0 308 53 also also RB cord-319626-f1b3ygg0 308 54 can can MD cord-319626-f1b3ygg0 308 55 readily readily RB cord-319626-f1b3ygg0 308 56 be be VB cord-319626-f1b3ygg0 308 57 detected detect VBN cord-319626-f1b3ygg0 308 58 by by IN cord-319626-f1b3ygg0 308 59 immunocytochemistry immunocytochemistry NNP cord-319626-f1b3ygg0 309 1 ( ( -LRB- cord-319626-f1b3ygg0 309 2 Tooze Tooze NNP cord-319626-f1b3ygg0 309 3 et et NNP cord-319626-f1b3ygg0 309 4 al al NNP cord-319626-f1b3ygg0 309 5 . . NNP cord-319626-f1b3ygg0 309 6 , , , cord-319626-f1b3ygg0 309 7 1985 1985 CD cord-319626-f1b3ygg0 309 8 ) ) -RRB- cord-319626-f1b3ygg0 309 9 . . . cord-319626-f1b3ygg0 310 1 However however RB cord-319626-f1b3ygg0 310 2 , , , cord-319626-f1b3ygg0 310 3 the the DT cord-319626-f1b3ygg0 310 4 constant constant JJ cord-319626-f1b3ygg0 310 5 amount amount NN cord-319626-f1b3ygg0 310 6 of of IN cord-319626-f1b3ygg0 310 7 E1 e1 NN cord-319626-f1b3ygg0 310 8 containing contain VBG cord-319626-f1b3ygg0 310 9 GalNAc GalNAc NNP cord-319626-f1b3ygg0 310 10 ( ( -LRB- cord-319626-f1b3ygg0 310 11 Eli Eli NNP cord-319626-f1b3ygg0 310 12 ) ) -RRB- cord-319626-f1b3ygg0 310 13 implies imply VBZ cord-319626-f1b3ygg0 310 14 that that IN cord-319626-f1b3ygg0 310 15 the the DT cord-319626-f1b3ygg0 310 16 compartment compartment NN cord-319626-f1b3ygg0 310 17 in in IN cord-319626-f1b3ygg0 310 18 which which WDT cord-319626-f1b3ygg0 310 19 the the DT cord-319626-f1b3ygg0 310 20 sugar sugar NN cord-319626-f1b3ygg0 310 21 is be VBZ cord-319626-f1b3ygg0 310 22 added add VBN cord-319626-f1b3ygg0 310 23 has have VBZ cord-319626-f1b3ygg0 310 24 a a DT cord-319626-f1b3ygg0 310 25 limited limited JJ cord-319626-f1b3ygg0 310 26 capacity capacity NN cord-319626-f1b3ygg0 310 27 and and CC cord-319626-f1b3ygg0 310 28 is be VBZ cord-319626-f1b3ygg0 310 29 smaller small JJR cord-319626-f1b3ygg0 310 30 than than IN cord-319626-f1b3ygg0 310 31 the the DT cord-319626-f1b3ygg0 310 32 rough rough JJ cord-319626-f1b3ygg0 310 33 ER er NN cord-319626-f1b3ygg0 310 34 . . . cord-319626-f1b3ygg0 311 1 Morphological morphological JJ cord-319626-f1b3ygg0 311 2 evidence evidence NN cord-319626-f1b3ygg0 311 3 indicates indicate VBZ cord-319626-f1b3ygg0 311 4 that that IN cord-319626-f1b3ygg0 311 5 the the DT cord-319626-f1b3ygg0 311 6 budding bud VBG cord-319626-f1b3ygg0 311 7 compartment compartment NN cord-319626-f1b3ygg0 311 8 fulfills fulfill VBZ cord-319626-f1b3ygg0 311 9 these these DT cord-319626-f1b3ygg0 311 10 criteria criterion NNS cord-319626-f1b3ygg0 311 11 . . . cord-319626-f1b3ygg0 312 1 The the DT cord-319626-f1b3ygg0 312 2 budding bud VBG cord-319626-f1b3ygg0 312 3 compartment compartment NN cord-319626-f1b3ygg0 312 4 is be VBZ cord-319626-f1b3ygg0 312 5 not not RB cord-319626-f1b3ygg0 312 6 a a DT cord-319626-f1b3ygg0 312 7 product product NN cord-319626-f1b3ygg0 312 8 of of IN cord-319626-f1b3ygg0 312 9 infection infection NN cord-319626-f1b3ygg0 312 10 because because IN cord-319626-f1b3ygg0 312 11 it -PRON- PRP cord-319626-f1b3ygg0 312 12 is be VBZ cord-319626-f1b3ygg0 312 13 found find VBN cord-319626-f1b3ygg0 312 14 in in IN cord-319626-f1b3ygg0 312 15 uninfected uninfected JJ cord-319626-f1b3ygg0 312 16 sac(- sac(- NNS cord-319626-f1b3ygg0 312 17 ) ) -RRB- cord-319626-f1b3ygg0 312 18 cells cell NNS cord-319626-f1b3ygg0 312 19 and and CC cord-319626-f1b3ygg0 312 20 our -PRON- PRP$ cord-319626-f1b3ygg0 312 21 preliminary preliminary JJ cord-319626-f1b3ygg0 312 22 observations observation NNS cord-319626-f1b3ygg0 312 23 of of IN cord-319626-f1b3ygg0 312 24 serial serial JJ cord-319626-f1b3ygg0 312 25 sections section NNS cord-319626-f1b3ygg0 312 26 of of IN cord-319626-f1b3ygg0 312 27 rat rat NN cord-319626-f1b3ygg0 312 28 exocrine exocrine JJ cord-319626-f1b3ygg0 312 29 pancreatic pancreatic NN cord-319626-f1b3ygg0 312 30 cells cell NNS cord-319626-f1b3ygg0 312 31 and and CC cord-319626-f1b3ygg0 312 32 murine murine JJ cord-319626-f1b3ygg0 312 33 AtT20 AtT20 NNP cord-319626-f1b3ygg0 312 34 cells cell NNS cord-319626-f1b3ygg0 312 35 shows show VBZ cord-319626-f1b3ygg0 312 36 that that IN cord-319626-f1b3ygg0 312 37 it -PRON- PRP cord-319626-f1b3ygg0 312 38 is be VBZ cord-319626-f1b3ygg0 312 39 not not RB cord-319626-f1b3ygg0 312 40 peculiar peculiar JJ cord-319626-f1b3ygg0 312 41 to to IN cord-319626-f1b3ygg0 312 42 sac(- sac(- NNP cord-319626-f1b3ygg0 312 43 ) ) -RRB- cord-319626-f1b3ygg0 312 44 cells cell NNS cord-319626-f1b3ygg0 312 45 . . . cord-319626-f1b3ygg0 313 1 In in IN cord-319626-f1b3ygg0 313 2 all all DT cord-319626-f1b3ygg0 313 3 three three CD cord-319626-f1b3ygg0 313 4 cell cell NN cord-319626-f1b3ygg0 313 5 types type NNS cord-319626-f1b3ygg0 313 6 , , , cord-319626-f1b3ygg0 313 7 the the DT cord-319626-f1b3ygg0 313 8 budding bud VBG cord-319626-f1b3ygg0 313 9 compartment compartment NN cord-319626-f1b3ygg0 313 10 is be VBZ cord-319626-f1b3ygg0 313 11 found find VBN cord-319626-f1b3ygg0 313 12 frequently frequently RB cord-319626-f1b3ygg0 313 13 in in IN cord-319626-f1b3ygg0 313 14 close close JJ cord-319626-f1b3ygg0 313 15 association association NN cord-319626-f1b3ygg0 313 16 with with IN cord-319626-f1b3ygg0 313 17 patches patch NNS cord-319626-f1b3ygg0 313 18 of of IN cord-319626-f1b3ygg0 313 19 rough rough JJ cord-319626-f1b3ygg0 313 20 ER ER NNP cord-319626-f1b3ygg0 313 21 differentiated differentiate VBN cord-319626-f1b3ygg0 313 22 into into IN cord-319626-f1b3ygg0 313 23 transitional transitional JJ cord-319626-f1b3ygg0 313 24 elements element NNS cord-319626-f1b3ygg0 313 25 in in IN cord-319626-f1b3ygg0 313 26 regions region NNS cord-319626-f1b3ygg0 313 27 juxtaposed juxtapose VBN cord-319626-f1b3ygg0 313 28 to to IN cord-319626-f1b3ygg0 313 29 the the DT cord-319626-f1b3ygg0 313 30 cis cis NNP cord-319626-f1b3ygg0 313 31 side side NN cord-319626-f1b3ygg0 313 32 of of IN cord-319626-f1b3ygg0 313 33 the the DT cord-319626-f1b3ygg0 313 34 Golgi Golgi NNP cord-319626-f1b3ygg0 313 35 apparatus apparatus NN cord-319626-f1b3ygg0 313 36 . . . cord-319626-f1b3ygg0 314 1 However however RB cord-319626-f1b3ygg0 314 2 , , , cord-319626-f1b3ygg0 314 3 the the DT cord-319626-f1b3ygg0 314 4 budding bud VBG cord-319626-f1b3ygg0 314 5 compartment compartment NN cord-319626-f1b3ygg0 314 6 and and CC cord-319626-f1b3ygg0 314 7 transitional transitional JJ cord-319626-f1b3ygg0 314 8 elements element NNS cord-319626-f1b3ygg0 314 9 of of IN cord-319626-f1b3ygg0 314 10 the the DT cord-319626-f1b3ygg0 314 11 rough rough JJ cord-319626-f1b3ygg0 314 12 ER er NN cord-319626-f1b3ygg0 314 13 in in IN cord-319626-f1b3ygg0 314 14 sac(- sac(- NN cord-319626-f1b3ygg0 314 15 ) ) -RRB- cord-319626-f1b3ygg0 314 16 cells cell NNS cord-319626-f1b3ygg0 314 17 are be VBP cord-319626-f1b3ygg0 314 18 not not RB cord-319626-f1b3ygg0 314 19 restricted restrict VBN cord-319626-f1b3ygg0 314 20 to to IN cord-319626-f1b3ygg0 314 21 this this DT cord-319626-f1b3ygg0 314 22 region region NN cord-319626-f1b3ygg0 314 23 ; ; : cord-319626-f1b3ygg0 314 24 they -PRON- PRP cord-319626-f1b3ygg0 314 25 also also RB cord-319626-f1b3ygg0 314 26 occur occur VBP cord-319626-f1b3ygg0 314 27 associated associate VBN cord-319626-f1b3ygg0 314 28 with with IN cord-319626-f1b3ygg0 314 29 patches patch NNS cord-319626-f1b3ygg0 314 30 of of IN cord-319626-f1b3ygg0 314 31 rough rough JJ cord-319626-f1b3ygg0 314 32 ER ER NNP cord-319626-f1b3ygg0 314 33 remote remote NN cord-319626-f1b3ygg0 314 34 from from IN cord-319626-f1b3ygg0 314 35 the the DT cord-319626-f1b3ygg0 314 36 Golgi Golgi NNP cord-319626-f1b3ygg0 314 37 apparatus apparatus NN cord-319626-f1b3ygg0 314 38 . . . cord-319626-f1b3ygg0 315 1 The the DT cord-319626-f1b3ygg0 315 2 existence existence NN cord-319626-f1b3ygg0 315 3 of of IN cord-319626-f1b3ygg0 315 4 peripheral peripheral JJ cord-319626-f1b3ygg0 315 5 transitional transitional JJ cord-319626-f1b3ygg0 315 6 elements element NNS cord-319626-f1b3ygg0 315 7 in in IN cord-319626-f1b3ygg0 315 8 pancreatic pancreatic JJ cord-319626-f1b3ygg0 315 9 cells cell NNS cord-319626-f1b3ygg0 315 10 has have VBZ cord-319626-f1b3ygg0 315 11 long long RB cord-319626-f1b3ygg0 315 12 been be VBN cord-319626-f1b3ygg0 315 13 known know VBN cord-319626-f1b3ygg0 315 14 ( ( -LRB- cord-319626-f1b3ygg0 315 15 Jamieson Jamieson NNP cord-319626-f1b3ygg0 315 16 and and CC cord-319626-f1b3ygg0 315 17 Palade Palade NNP cord-319626-f1b3ygg0 315 18 , , , cord-319626-f1b3ygg0 315 19 1967 1967 CD cord-319626-f1b3ygg0 315 20 ) ) -RRB- cord-319626-f1b3ygg0 315 21 . . . cord-319626-f1b3ygg0 316 1 Our -PRON- PRP$ cord-319626-f1b3ygg0 316 2 observations observation NNS cord-319626-f1b3ygg0 316 3 , , , cord-319626-f1b3ygg0 316 4 together together RB cord-319626-f1b3ygg0 316 5 with with IN cord-319626-f1b3ygg0 316 6 those those DT cord-319626-f1b3ygg0 316 7 of of IN cord-319626-f1b3ygg0 316 8 Saraste Saraste NNP cord-319626-f1b3ygg0 316 9 and and CC cord-319626-f1b3ygg0 316 10 Kuismanen Kuismanen NNP cord-319626-f1b3ygg0 316 11 ( ( -LRB- cord-319626-f1b3ygg0 316 12 1984 1984 CD cord-319626-f1b3ygg0 316 13 ) ) -RRB- cord-319626-f1b3ygg0 316 14 , , , cord-319626-f1b3ygg0 316 15 Tartakoff Tartakoff NNP cord-319626-f1b3ygg0 316 16 ( ( -LRB- cord-319626-f1b3ygg0 316 17 1986 1986 CD cord-319626-f1b3ygg0 316 18 ) ) -RRB- cord-319626-f1b3ygg0 316 19 , , , cord-319626-f1b3ygg0 316 20 and and CC cord-319626-f1b3ygg0 316 21 Merisko Merisko NNP cord-319626-f1b3ygg0 316 22 et et NNP cord-319626-f1b3ygg0 316 23 al al NNP cord-319626-f1b3ygg0 316 24 . . . cord-319626-f1b3ygg0 317 1 ( ( -LRB- cord-319626-f1b3ygg0 317 2 1986 1986 CD cord-319626-f1b3ygg0 317 3 ) ) -RRB- cord-319626-f1b3ygg0 317 4 indicate indicate VBP cord-319626-f1b3ygg0 317 5 that that IN cord-319626-f1b3ygg0 317 6 the the DT cord-319626-f1b3ygg0 317 7 structure structure NN cord-319626-f1b3ygg0 317 8 of of IN cord-319626-f1b3ygg0 317 9 the the DT cord-319626-f1b3ygg0 317 10 transitional transitional JJ cord-319626-f1b3ygg0 317 11 element element NN cord-319626-f1b3ygg0 317 12 - - HYPH cord-319626-f1b3ygg0 317 13 transitional transitional JJ cord-319626-f1b3ygg0 317 14 vesicle vesicle NN cord-319626-f1b3ygg0 317 15 region region NN cord-319626-f1b3ygg0 317 16 of of IN cord-319626-f1b3ygg0 317 17 the the DT cord-319626-f1b3ygg0 317 18 cell cell NN cord-319626-f1b3ygg0 317 19 is be VBZ cord-319626-f1b3ygg0 317 20 considerably considerably RB cord-319626-f1b3ygg0 317 21 more more RBR cord-319626-f1b3ygg0 317 22 complex complex JJ cord-319626-f1b3ygg0 317 23 than than IN cord-319626-f1b3ygg0 317 24 hitherto hitherto VBN cord-319626-f1b3ygg0 317 25 appreciated appreciate VBN cord-319626-f1b3ygg0 317 26 . . . cord-319626-f1b3ygg0 318 1 Fig Fig NNP cord-319626-f1b3ygg0 318 2 . . . cord-319626-f1b3ygg0 319 1 12 12 CD cord-319626-f1b3ygg0 319 2 summarizes summarize VBZ cord-319626-f1b3ygg0 319 3 our -PRON- PRP$ cord-319626-f1b3ygg0 319 4 interpretation interpretation NN cord-319626-f1b3ygg0 319 5 of of IN cord-319626-f1b3ygg0 319 6 the the DT cord-319626-f1b3ygg0 319 7 results result NNS cord-319626-f1b3ygg0 319 8 described describe VBN cord-319626-f1b3ygg0 319 9 above above IN cord-319626-f1b3ygg0 319 10 which which WDT cord-319626-f1b3ygg0 319 11 suggest suggest VBP cord-319626-f1b3ygg0 319 12 that that IN cord-319626-f1b3ygg0 319 13 GalNAc GalNAc NNP cord-319626-f1b3ygg0 319 14 is be VBZ cord-319626-f1b3ygg0 319 15 added add VBN cord-319626-f1b3ygg0 319 16 in in IN cord-319626-f1b3ygg0 319 17 the the DT cord-319626-f1b3ygg0 319 18 budding bud VBG cord-319626-f1b3ygg0 319 19 compartment compartment NN cord-319626-f1b3ygg0 319 20 . . . cord-319626-f1b3ygg0 320 1 This this DT cord-319626-f1b3ygg0 320 2 is be VBZ cord-319626-f1b3ygg0 320 3 not not RB cord-319626-f1b3ygg0 320 4 to to TO cord-319626-f1b3ygg0 320 5 say say VB cord-319626-f1b3ygg0 320 6 that that IN cord-319626-f1b3ygg0 320 7 the the DT cord-319626-f1b3ygg0 320 8 enzyme enzyme NN cord-319626-f1b3ygg0 320 9 , , , cord-319626-f1b3ygg0 320 10 N n CD cord-319626-f1b3ygg0 320 11 - - HYPH cord-319626-f1b3ygg0 320 12 acetyl acetyl NNP cord-319626-f1b3ygg0 320 13 - - HYPH cord-319626-f1b3ygg0 320 14 galactosaminyltransferase galactosaminyltransferase NNP cord-319626-f1b3ygg0 320 15 , , , cord-319626-f1b3ygg0 320 16 is be VBZ cord-319626-f1b3ygg0 320 17 found find VBN cord-319626-f1b3ygg0 320 18 exclusively exclusively RB cord-319626-f1b3ygg0 320 19 in in IN cord-319626-f1b3ygg0 320 20 this this DT cord-319626-f1b3ygg0 320 21 compartment compartment NN cord-319626-f1b3ygg0 320 22 , , , cord-319626-f1b3ygg0 320 23 although although IN cord-319626-f1b3ygg0 320 24 available available JJ cord-319626-f1b3ygg0 320 25 fractionation fractionation NN cord-319626-f1b3ygg0 320 26 data datum NNS cord-319626-f1b3ygg0 320 27 shows show VBZ cord-319626-f1b3ygg0 320 28 that that IN cord-319626-f1b3ygg0 320 29 it -PRON- PRP cord-319626-f1b3ygg0 320 30 is be VBZ cord-319626-f1b3ygg0 320 31 restricted restrict VBN cord-319626-f1b3ygg0 320 32 to to IN cord-319626-f1b3ygg0 320 33 smooth smooth JJ cord-319626-f1b3ygg0 320 34 membranes membrane NNS cord-319626-f1b3ygg0 320 35 ( ( -LRB- cord-319626-f1b3ygg0 320 36 Kim Kim NNP cord-319626-f1b3ygg0 320 37 et et NNP cord-319626-f1b3ygg0 320 38 al al NNP cord-319626-f1b3ygg0 320 39 . . NNP cord-319626-f1b3ygg0 320 40 , , , cord-319626-f1b3ygg0 320 41 1971 1971 CD cord-319626-f1b3ygg0 320 42 ; ; : cord-319626-f1b3ygg0 320 43 Ko Ko NNP cord-319626-f1b3ygg0 320 44 and and CC cord-319626-f1b3ygg0 320 45 Raghupathy Raghupathy NNP cord-319626-f1b3ygg0 320 46 , , , cord-319626-f1b3ygg0 320 47 1972 1972 CD cord-319626-f1b3ygg0 320 48 ; ; : cord-319626-f1b3ygg0 320 49 Hanover Hanover NNP cord-319626-f1b3ygg0 320 50 et et NNP cord-319626-f1b3ygg0 320 51 al al NNP cord-319626-f1b3ygg0 320 52 . . NNP cord-319626-f1b3ygg0 320 53 , , , cord-319626-f1b3ygg0 320 54 1980 1980 CD cord-319626-f1b3ygg0 321 1 Hanover Hanover NNP cord-319626-f1b3ygg0 321 2 et et NNP cord-319626-f1b3ygg0 321 3 al al NNP cord-319626-f1b3ygg0 321 4 . . . cord-319626-f1b3ygg0 322 1 , , , cord-319626-f1b3ygg0 322 2 , , , cord-319626-f1b3ygg0 322 3 1982 1982 CD cord-319626-f1b3ygg0 322 4 Elhammer Elhammer NNP cord-319626-f1b3ygg0 322 5 and and CC cord-319626-f1b3ygg0 322 6 Kornfeld Kornfeld NNP cord-319626-f1b3ygg0 322 7 , , , cord-319626-f1b3ygg0 322 8 1984 1984 CD cord-319626-f1b3ygg0 322 9 ) ) -RRB- cord-319626-f1b3ygg0 322 10 . . . cord-319626-f1b3ygg0 323 1 One one PRP cord-319626-f1b3ygg0 323 2 must must MD cord-319626-f1b3ygg0 323 3 remember remember VB cord-319626-f1b3ygg0 323 4 that that IN cord-319626-f1b3ygg0 323 5 the the DT cord-319626-f1b3ygg0 323 6 position position NN cord-319626-f1b3ygg0 323 7 of of IN cord-319626-f1b3ygg0 323 8 an an DT cord-319626-f1b3ygg0 323 9 enzyme enzyme NN cord-319626-f1b3ygg0 323 10 can can MD cord-319626-f1b3ygg0 323 11 only only RB cord-319626-f1b3ygg0 323 12 be be VB cord-319626-f1b3ygg0 323 13 inferred infer VBN cord-319626-f1b3ygg0 323 14 from from IN cord-319626-f1b3ygg0 323 15 the the DT cord-319626-f1b3ygg0 323 16 position position NN cord-319626-f1b3ygg0 323 17 of of IN cord-319626-f1b3ygg0 323 18 the the DT cord-319626-f1b3ygg0 323 19 substrate substrate NN cord-319626-f1b3ygg0 323 20 and and CC cord-319626-f1b3ygg0 323 21 product product NN cord-319626-f1b3ygg0 323 22 subject subject NN cord-319626-f1b3ygg0 323 23 to to IN cord-319626-f1b3ygg0 323 24 certain certain JJ cord-319626-f1b3ygg0 323 25 strict strict JJ cord-319626-f1b3ygg0 323 26 criteria criterion NNS cord-319626-f1b3ygg0 323 27 . . . cord-319626-f1b3ygg0 324 1 Obviously obviously RB cord-319626-f1b3ygg0 324 2 , , , cord-319626-f1b3ygg0 324 3 the the DT cord-319626-f1b3ygg0 324 4 enzyme enzyme NN cord-319626-f1b3ygg0 324 5 can can MD cord-319626-f1b3ygg0 324 6 not not RB cord-319626-f1b3ygg0 324 7 be be VB cord-319626-f1b3ygg0 324 8 located locate VBN cord-319626-f1b3ygg0 324 9 in in IN cord-319626-f1b3ygg0 324 10 the the DT cord-319626-f1b3ygg0 324 11 pathway pathway NN cord-319626-f1b3ygg0 324 12 after after IN cord-319626-f1b3ygg0 324 13 the the DT cord-319626-f1b3ygg0 324 14 point point NN cord-319626-f1b3ygg0 324 15 at at IN cord-319626-f1b3ygg0 324 16 which which WDT cord-319626-f1b3ygg0 324 17 the the DT cord-319626-f1b3ygg0 324 18 substrate substrate NN cord-319626-f1b3ygg0 324 19 acquires acquire VBZ cord-319626-f1b3ygg0 324 20 the the DT cord-319626-f1b3ygg0 324 21 sugar sugar NN cord-319626-f1b3ygg0 324 22 , , , cord-319626-f1b3ygg0 324 23 but but CC cord-319626-f1b3ygg0 324 24 it -PRON- PRP cord-319626-f1b3ygg0 324 25 can can MD cord-319626-f1b3ygg0 324 26 exist exist VB cord-319626-f1b3ygg0 324 27 at at IN cord-319626-f1b3ygg0 324 28 any any DT cord-319626-f1b3ygg0 324 29 site site NN cord-319626-f1b3ygg0 324 30 up up IN cord-319626-f1b3ygg0 324 31 to to IN cord-319626-f1b3ygg0 324 32 the the DT cord-319626-f1b3ygg0 324 33 point point NN cord-319626-f1b3ygg0 324 34 at at IN cord-319626-f1b3ygg0 324 35 which which WDT cord-319626-f1b3ygg0 324 36 the the DT cord-319626-f1b3ygg0 324 37 substrate substrate NN cord-319626-f1b3ygg0 324 38 appears appear VBZ cord-319626-f1b3ygg0 324 39 to to TO cord-319626-f1b3ygg0 324 40 be be VB cord-319626-f1b3ygg0 324 41 modified modify VBN cord-319626-f1b3ygg0 324 42 . . . cord-319626-f1b3ygg0 325 1 This this DT cord-319626-f1b3ygg0 325 2 is be VBZ cord-319626-f1b3ygg0 325 3 because because IN cord-319626-f1b3ygg0 325 4 the the DT cord-319626-f1b3ygg0 325 5 substate substate NN cord-319626-f1b3ygg0 325 6 may may MD cord-319626-f1b3ygg0 325 7 have have VB cord-319626-f1b3ygg0 325 8 to to TO cord-319626-f1b3ygg0 325 9 assume assume VB cord-319626-f1b3ygg0 325 10 a a DT cord-319626-f1b3ygg0 325 11 particular particular JJ cord-319626-f1b3ygg0 325 12 conformation conformation NN cord-319626-f1b3ygg0 325 13 before before IN cord-319626-f1b3ygg0 325 14 it -PRON- PRP cord-319626-f1b3ygg0 325 15 can can MD cord-319626-f1b3ygg0 325 16 be be VB cord-319626-f1b3ygg0 325 17 acted act VBN cord-319626-f1b3ygg0 325 18 upon upon IN cord-319626-f1b3ygg0 325 19 by by IN cord-319626-f1b3ygg0 325 20 the the DT cord-319626-f1b3ygg0 325 21 enzyme enzyme NN cord-319626-f1b3ygg0 325 22 . . . cord-319626-f1b3ygg0 326 1 The the DT cord-319626-f1b3ygg0 326 2 N N NNP cord-319626-f1b3ygg0 326 3 - - HYPH cord-319626-f1b3ygg0 326 4 acetyl acetyl NN cord-319626-f1b3ygg0 326 5 - - HYPH cord-319626-f1b3ygg0 326 6 galactosaminyltransferase galactosaminyltransferase NNP cord-319626-f1b3ygg0 326 7 could could MD cord-319626-f1b3ygg0 326 8 be be VB cord-319626-f1b3ygg0 326 9 present present JJ cord-319626-f1b3ygg0 326 10 earlier early RBR cord-319626-f1b3ygg0 326 11 in in IN cord-319626-f1b3ygg0 326 12 the the DT cord-319626-f1b3ygg0 326 13 pathway pathway NN cord-319626-f1b3ygg0 326 14 than than IN cord-319626-f1b3ygg0 326 15 the the DT cord-319626-f1b3ygg0 326 16 budding bud VBG cord-319626-f1b3ygg0 326 17 compartment compartment NN cord-319626-f1b3ygg0 326 18 but but CC cord-319626-f1b3ygg0 326 19 the the DT cord-319626-f1b3ygg0 326 20 E1 e1 NN cord-319626-f1b3ygg0 326 21 may may MD cord-319626-f1b3ygg0 326 22 not not RB cord-319626-f1b3ygg0 326 23 be be VB cord-319626-f1b3ygg0 326 24 a a DT cord-319626-f1b3ygg0 326 25 substrate substrate NN cord-319626-f1b3ygg0 326 26 until until IN cord-319626-f1b3ygg0 326 27 it -PRON- PRP cord-319626-f1b3ygg0 326 28 reaches reach VBZ cord-319626-f1b3ygg0 326 29 the the DT cord-319626-f1b3ygg0 326 30 budding bud VBG cord-319626-f1b3ygg0 326 31 compartment compartment NN cord-319626-f1b3ygg0 326 32 . . . cord-319626-f1b3ygg0 327 1 Alternatively alternatively RB cord-319626-f1b3ygg0 327 2 , , , cord-319626-f1b3ygg0 327 3 the the DT cord-319626-f1b3ygg0 327 4 means mean NNS cord-319626-f1b3ygg0 327 5 of of IN cord-319626-f1b3ygg0 327 6 detecting detect VBG cord-319626-f1b3ygg0 327 7 the the DT cord-319626-f1b3ygg0 327 8 modification modification NN cord-319626-f1b3ygg0 327 9 may may MD cord-319626-f1b3ygg0 327 10 depend depend VB cord-319626-f1b3ygg0 327 11 on on IN cord-319626-f1b3ygg0 327 12 the the DT cord-319626-f1b3ygg0 327 13 substrate substrate NN cord-319626-f1b3ygg0 327 14 conformation conformation NN cord-319626-f1b3ygg0 327 15 . . . cord-319626-f1b3ygg0 328 1 The the DT cord-319626-f1b3ygg0 328 2 presence presence NN cord-319626-f1b3ygg0 328 3 of of IN cord-319626-f1b3ygg0 328 4 protein protein NN cord-319626-f1b3ygg0 328 5 - - HYPH cord-319626-f1b3ygg0 328 6 bound bind VBN cord-319626-f1b3ygg0 328 7 GalNAc galnac NN cord-319626-f1b3ygg0 328 8 in in IN cord-319626-f1b3ygg0 328 9 the the DT cord-319626-f1b3ygg0 328 10 cis cis NNP cord-319626-f1b3ygg0 328 11 Golgi Golgi NNP cord-319626-f1b3ygg0 328 12 as as IN cord-319626-f1b3ygg0 328 13 detected detect VBN cord-319626-f1b3ygg0 328 14 by by IN cord-319626-f1b3ygg0 328 15 lectin lectin NN cord-319626-f1b3ygg0 328 16 labeling labeling NN cord-319626-f1b3ygg0 328 17 ( ( -LRB- cord-319626-f1b3ygg0 328 18 Roth Roth NNP cord-319626-f1b3ygg0 328 19 , , , cord-319626-f1b3ygg0 328 20 1984 1984 CD cord-319626-f1b3ygg0 328 21 ) ) -RRB- cord-319626-f1b3ygg0 328 22 may may MD cord-319626-f1b3ygg0 328 23 reflect reflect VB cord-319626-f1b3ygg0 328 24 addition addition NN cord-319626-f1b3ygg0 328 25 of of IN cord-319626-f1b3ygg0 328 26 the the DT cord-319626-f1b3ygg0 328 27 sugar sugar NN cord-319626-f1b3ygg0 328 28 in in IN cord-319626-f1b3ygg0 328 29 this this DT cord-319626-f1b3ygg0 328 30 compartment compartment NN cord-319626-f1b3ygg0 328 31 or or CC cord-319626-f1b3ygg0 328 32 in in IN cord-319626-f1b3ygg0 328 33 some some DT cord-319626-f1b3ygg0 328 34 prior prior JJ cord-319626-f1b3ygg0 328 35 compartment compartment NN cord-319626-f1b3ygg0 328 36 in in IN cord-319626-f1b3ygg0 328 37 which which WDT cord-319626-f1b3ygg0 328 38 the the DT cord-319626-f1b3ygg0 328 39 protein protein NN cord-319626-f1b3ygg0 328 40 - - HYPH cord-319626-f1b3ygg0 328 41 bound bind VBN cord-319626-f1b3ygg0 328 42 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 328 43 is be VBZ cord-319626-f1b3ygg0 328 44 inaccessible inaccessible JJ cord-319626-f1b3ygg0 328 45 to to IN cord-319626-f1b3ygg0 328 46 the the DT cord-319626-f1b3ygg0 328 47 lectin lectin NN cord-319626-f1b3ygg0 328 48 . . . cord-319626-f1b3ygg0 329 1 These these DT cord-319626-f1b3ygg0 329 2 possibilities possibility NNS cord-319626-f1b3ygg0 329 3 are be VBP cord-319626-f1b3ygg0 329 4 not not RB cord-319626-f1b3ygg0 329 5 normally normally RB cord-319626-f1b3ygg0 329 6 considered consider VBN cord-319626-f1b3ygg0 329 7 but but CC cord-319626-f1b3ygg0 329 8 they -PRON- PRP cord-319626-f1b3ygg0 329 9 could could MD cord-319626-f1b3ygg0 329 10 explain explain VB cord-319626-f1b3ygg0 329 11 the the DT cord-319626-f1b3ygg0 329 12 large large JJ cord-319626-f1b3ygg0 329 13 and and CC cord-319626-f1b3ygg0 329 14 otherwise otherwise RB cord-319626-f1b3ygg0 329 15 irreconcilable irreconcilable JJ cord-319626-f1b3ygg0 329 16 discrepancies discrepancy NNS cord-319626-f1b3ygg0 329 17 in in IN cord-319626-f1b3ygg0 329 18 the the DT cord-319626-f1b3ygg0 329 19 location location NN cord-319626-f1b3ygg0 329 20 that that WDT cord-319626-f1b3ygg0 329 21 has have VBZ cord-319626-f1b3ygg0 329 22 been be VBN cord-319626-f1b3ygg0 329 23 assigned assign VBN cord-319626-f1b3ygg0 329 24 to to IN cord-319626-f1b3ygg0 329 25 N n NN cord-319626-f1b3ygg0 329 26 - - HYPH cord-319626-f1b3ygg0 329 27 acetyl acetyl NN cord-319626-f1b3ygg0 329 28 - - HYPH cord-319626-f1b3ygg0 329 29 galactosaminyltransferase galactosaminyltransferase NN cord-319626-f1b3ygg0 329 30 ( ( -LRB- cord-319626-f1b3ygg0 329 31 Strous Strous NNP cord-319626-f1b3ygg0 329 32 , , , cord-319626-f1b3ygg0 329 33 1979 1979 CD cord-319626-f1b3ygg0 329 34 ; ; : cord-319626-f1b3ygg0 330 1 Niemann Niemann NNP cord-319626-f1b3ygg0 330 2 et et NNP cord-319626-f1b3ygg0 330 3 al al NNP cord-319626-f1b3ygg0 330 4 . . . cord-319626-f1b3ygg0 331 1 , , , cord-319626-f1b3ygg0 331 2 1982 1982 CD cord-319626-f1b3ygg0 331 3 ; ; : cord-319626-f1b3ygg0 331 4 Cummings Cummings NNP cord-319626-f1b3ygg0 331 5 et et FW cord-319626-f1b3ygg0 331 6 al al NNP cord-319626-f1b3ygg0 331 7 . . . cord-319626-f1b3ygg0 332 1 , , , cord-319626-f1b3ygg0 332 2 Figure figure NN cord-319626-f1b3ygg0 332 3 12 12 CD cord-319626-f1b3ygg0 332 4 . . . cord-319626-f1b3ygg0 333 1 Schematic schematic JJ cord-319626-f1b3ygg0 333 2 illustrating illustrate VBG cord-319626-f1b3ygg0 333 3 the the DT cord-319626-f1b3ygg0 333 4 first first JJ cord-319626-f1b3ygg0 333 5 site site NN cord-319626-f1b3ygg0 333 6 of of IN cord-319626-f1b3ygg0 333 7 budding bud VBG cord-319626-f1b3ygg0 333 8 of of IN cord-319626-f1b3ygg0 333 9 MHV MHV NNP cord-319626-f1b3ygg0 333 10 - - HYPH cord-319626-f1b3ygg0 333 11 A59 A59 NNP cord-319626-f1b3ygg0 333 12 into into IN cord-319626-f1b3ygg0 333 13 a a DT cord-319626-f1b3ygg0 333 14 smooth smooth JJ cord-319626-f1b3ygg0 333 15 membrane membrane NN cord-319626-f1b3ygg0 333 16 compartment compartment NN cord-319626-f1b3ygg0 333 17 between between IN cord-319626-f1b3ygg0 333 18 the the DT cord-319626-f1b3ygg0 333 19 transitional transitional JJ cord-319626-f1b3ygg0 333 20 elements element NNS cord-319626-f1b3ygg0 333 21 of of IN cord-319626-f1b3ygg0 333 22 the the DT cord-319626-f1b3ygg0 333 23 ER ER NNP cord-319626-f1b3ygg0 333 24 and and CC cord-319626-f1b3ygg0 333 25 the the DT cord-319626-f1b3ygg0 333 26 Golgi Golgi NNP cord-319626-f1b3ygg0 333 27 stack stack NN cord-319626-f1b3ygg0 333 28 . . . cord-319626-f1b3ygg0 334 1 Reduced reduce VBN cord-319626-f1b3ygg0 334 2 temperature temperature NN cord-319626-f1b3ygg0 334 3 blocks block NNS cord-319626-f1b3ygg0 334 4 entry entry NN cord-319626-f1b3ygg0 334 5 of of IN cord-319626-f1b3ygg0 334 6 the the DT cord-319626-f1b3ygg0 334 7 virions virion NNS cord-319626-f1b3ygg0 334 8 into into IN cord-319626-f1b3ygg0 334 9 the the DT cord-319626-f1b3ygg0 334 10 Golgi Golgi NNP cord-319626-f1b3ygg0 334 11 stack stack NN cord-319626-f1b3ygg0 334 12 and and CC cord-319626-f1b3ygg0 334 13 prevents prevent VBZ cord-319626-f1b3ygg0 334 14 El El NNP cord-319626-f1b3ygg0 334 15 acquiring acquire VBG cord-319626-f1b3ygg0 334 16 galactose galactose NN cord-319626-f1b3ygg0 334 17 and and CC cord-319626-f1b3ygg0 334 18 sialic sialic NN cord-319626-f1b3ygg0 334 19 acid acid NN cord-319626-f1b3ygg0 334 20 but but CC cord-319626-f1b3ygg0 334 21 not not RB cord-319626-f1b3ygg0 334 22 GalNAc GalNAc NNP cord-319626-f1b3ygg0 334 23 . . . cord-319626-f1b3ygg0 335 1 This this DT cord-319626-f1b3ygg0 335 2 sugar sugar NN cord-319626-f1b3ygg0 335 3 is be VBZ cord-319626-f1b3ygg0 335 4 probably probably RB cord-319626-f1b3ygg0 335 5 added add VBN cord-319626-f1b3ygg0 335 6 in in IN cord-319626-f1b3ygg0 335 7 the the DT cord-319626-f1b3ygg0 335 8 budding bud VBG cord-319626-f1b3ygg0 335 9 compartment compartment NN cord-319626-f1b3ygg0 335 10 which which WDT cord-319626-f1b3ygg0 335 11 is be VBZ cord-319626-f1b3ygg0 335 12 of of IN cord-319626-f1b3ygg0 335 13 limited limited JJ cord-319626-f1b3ygg0 335 14 size size NN cord-319626-f1b3ygg0 335 15 and and CC cord-319626-f1b3ygg0 335 16 distinct distinct JJ cord-319626-f1b3ygg0 335 17 from from IN cord-319626-f1b3ygg0 335 18 both both CC cord-319626-f1b3ygg0 335 19 the the DT cord-319626-f1b3ygg0 335 20 ER ER NNP cord-319626-f1b3ygg0 335 21 and and CC cord-319626-f1b3ygg0 335 22 Golgi Golgi NNP cord-319626-f1b3ygg0 335 23 apparatus apparatus NN cord-319626-f1b3ygg0 335 24 . . . cord-319626-f1b3ygg0 336 1 1983 1983 CD cord-319626-f1b3ygg0 337 1 ; ; : cord-319626-f1b3ygg0 337 2 Johnson Johnson NNP cord-319626-f1b3ygg0 337 3 and and CC cord-319626-f1b3ygg0 337 4 Spear Spear NNP cord-319626-f1b3ygg0 337 5 , , , cord-319626-f1b3ygg0 337 6 1983 1983 CD cord-319626-f1b3ygg0 337 7 ) ) -RRB- cord-319626-f1b3ygg0 337 8 . . . cord-319626-f1b3ygg0 338 1 These these DT cord-319626-f1b3ygg0 338 2 differences difference NNS cord-319626-f1b3ygg0 338 3 will will MD cord-319626-f1b3ygg0 338 4 only only RB cord-319626-f1b3ygg0 338 5 be be VB cord-319626-f1b3ygg0 338 6 resolved resolve VBN cord-319626-f1b3ygg0 338 7 by by IN cord-319626-f1b3ygg0 338 8 locating locate VBG cord-319626-f1b3ygg0 338 9 the the DT cord-319626-f1b3ygg0 338 10 enzyme enzyme NN cord-319626-f1b3ygg0 338 11 itself -PRON- PRP cord-319626-f1b3ygg0 338 12 using use VBG cord-319626-f1b3ygg0 338 13 an an DT cord-319626-f1b3ygg0 338 14 immunocytochemical immunocytochemical JJ cord-319626-f1b3ygg0 338 15 technique technique NN cord-319626-f1b3ygg0 338 16 . . . cord-319626-f1b3ygg0 339 1 The the DT cord-319626-f1b3ygg0 339 2 transport transport NN cord-319626-f1b3ygg0 339 3 to to IN cord-319626-f1b3ygg0 339 4 the the DT cord-319626-f1b3ygg0 339 5 plasma plasma NN cord-319626-f1b3ygg0 339 6 membrane membrane NN cord-319626-f1b3ygg0 339 7 of of IN cord-319626-f1b3ygg0 339 8 the the DT cord-319626-f1b3ygg0 339 9 envelope envelope NN cord-319626-f1b3ygg0 339 10 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 339 11 of of IN cord-319626-f1b3ygg0 339 12 the the DT cord-319626-f1b3ygg0 339 13 influenza influenza NN cord-319626-f1b3ygg0 339 14 virus virus NN cord-319626-f1b3ygg0 339 15 , , , cord-319626-f1b3ygg0 339 16 SFV SFV NNP cord-319626-f1b3ygg0 339 17 , , , cord-319626-f1b3ygg0 339 18 and and CC cord-319626-f1b3ygg0 339 19 VSV VSV NNP cord-319626-f1b3ygg0 339 20 can can MD cord-319626-f1b3ygg0 339 21 be be VB cord-319626-f1b3ygg0 339 22 slowed slow VBN cord-319626-f1b3ygg0 339 23 in in IN cord-319626-f1b3ygg0 339 24 the the DT cord-319626-f1b3ygg0 339 25 trans trans NN cord-319626-f1b3ygg0 339 26 - - JJ cord-319626-f1b3ygg0 339 27 most most JJS cord-319626-f1b3ygg0 339 28 Golgi Golgi NNP cord-319626-f1b3ygg0 339 29 compartment compartment NN cord-319626-f1b3ygg0 339 30 by by IN cord-319626-f1b3ygg0 339 31 reducing reduce VBG cord-319626-f1b3ygg0 339 32 the the DT cord-319626-f1b3ygg0 339 33 incubation incubation NN cord-319626-f1b3ygg0 339 34 temperature temperature NN cord-319626-f1b3ygg0 339 35 from from IN cord-319626-f1b3ygg0 339 36 37 37 CD cord-319626-f1b3ygg0 339 37 to to TO cord-319626-f1b3ygg0 339 38 20 20 CD cord-319626-f1b3ygg0 339 39 ° ° NN cord-319626-f1b3ygg0 339 40 C c NN cord-319626-f1b3ygg0 339 41 ( ( -LRB- cord-319626-f1b3ygg0 339 42 Matlin Matlin NNP cord-319626-f1b3ygg0 339 43 and and CC cord-319626-f1b3ygg0 339 44 Simons Simons NNP cord-319626-f1b3ygg0 339 45 , , , cord-319626-f1b3ygg0 339 46 1983 1983 CD cord-319626-f1b3ygg0 339 47 ; ; : cord-319626-f1b3ygg0 339 48 Saraste Saraste NNP cord-319626-f1b3ygg0 339 49 and and CC cord-319626-f1b3ygg0 339 50 Kuismanen Kuismanen NNP cord-319626-f1b3ygg0 339 51 , , , cord-319626-f1b3ygg0 339 52 1984 1984 CD cord-319626-f1b3ygg0 339 53 ; ; : cord-319626-f1b3ygg0 339 54 Fuller Fuller NNP cord-319626-f1b3ygg0 339 55 et et NNP cord-319626-f1b3ygg0 339 56 al al NNP cord-319626-f1b3ygg0 339 57 . . NNP cord-319626-f1b3ygg0 339 58 , , , cord-319626-f1b3ygg0 339 59 1985 1985 CD cord-319626-f1b3ygg0 339 60 ) ) -RRB- cord-319626-f1b3ygg0 339 61 . . . cord-319626-f1b3ygg0 340 1 Blocking block VBG cord-319626-f1b3ygg0 340 2 the the DT cord-319626-f1b3ygg0 340 3 transport transport NN cord-319626-f1b3ygg0 340 4 of of IN cord-319626-f1b3ygg0 340 5 these these DT cord-319626-f1b3ygg0 340 6 proteins protein NNS cord-319626-f1b3ygg0 340 7 from from IN cord-319626-f1b3ygg0 340 8 the the DT cord-319626-f1b3ygg0 340 9 rough rough JJ cord-319626-f1b3ygg0 340 10 ER er NN cord-319626-f1b3ygg0 340 11 to to IN cord-319626-f1b3ygg0 340 12 the the DT cord-319626-f1b3ygg0 340 13 Golgi Golgi NNP cord-319626-f1b3ygg0 340 14 stacks stack NNS cord-319626-f1b3ygg0 340 15 requires require VBZ cord-319626-f1b3ygg0 340 16 a a DT cord-319626-f1b3ygg0 340 17 further further JJ cord-319626-f1b3ygg0 340 18 reduction reduction NN cord-319626-f1b3ygg0 340 19 in in IN cord-319626-f1b3ygg0 340 20 temperature temperature NN cord-319626-f1b3ygg0 340 21 to to IN cord-319626-f1b3ygg0 340 22 16 16 CD cord-319626-f1b3ygg0 340 23 ° ° NN cord-319626-f1b3ygg0 340 24 C c NN cord-319626-f1b3ygg0 340 25 ( ( -LRB- cord-319626-f1b3ygg0 340 26 Saraste Saraste NNP cord-319626-f1b3ygg0 340 27 and and CC cord-319626-f1b3ygg0 340 28 Kuismanen Kuismanen NNP cord-319626-f1b3ygg0 340 29 , , , cord-319626-f1b3ygg0 340 30 1984 1984 CD cord-319626-f1b3ygg0 340 31 ) ) -RRB- cord-319626-f1b3ygg0 340 32 . . . cord-319626-f1b3ygg0 341 1 By by IN cord-319626-f1b3ygg0 341 2 contrast contrast NN cord-319626-f1b3ygg0 341 3 , , , cord-319626-f1b3ygg0 341 4 lowering lower VBG cord-319626-f1b3ygg0 341 5 the the DT cord-319626-f1b3ygg0 341 6 incubation incubation NN cord-319626-f1b3ygg0 341 7 temperature temperature NN cord-319626-f1b3ygg0 341 8 by by IN cord-319626-f1b3ygg0 341 9 only only RB cord-319626-f1b3ygg0 341 10 6 6 CD cord-319626-f1b3ygg0 341 11 ° ° NN cord-319626-f1b3ygg0 341 12 C c NN cord-319626-f1b3ygg0 341 13 , , , cord-319626-f1b3ygg0 341 14 from from IN cord-319626-f1b3ygg0 341 15 37 37 CD cord-319626-f1b3ygg0 341 16 to to TO cord-319626-f1b3ygg0 341 17 31 31 CD cord-319626-f1b3ygg0 341 18 ° ° NN cord-319626-f1b3ygg0 341 19 C c NN cord-319626-f1b3ygg0 341 20 , , , cord-319626-f1b3ygg0 341 21 results result VBZ cord-319626-f1b3ygg0 341 22 in in IN cord-319626-f1b3ygg0 341 23 at at IN cord-319626-f1b3ygg0 341 24 least least JJS cord-319626-f1b3ygg0 341 25 a a DT cord-319626-f1b3ygg0 341 26 1,000-fold 1,000-fold CD cord-319626-f1b3ygg0 341 27 reduction reduction NN cord-319626-f1b3ygg0 341 28 in in IN cord-319626-f1b3ygg0 341 29 the the DT cord-319626-f1b3ygg0 341 30 release release NN cord-319626-f1b3ygg0 341 31 of of IN cord-319626-f1b3ygg0 341 32 MHV MHV NNP cord-319626-f1b3ygg0 341 33 - - HYPH cord-319626-f1b3ygg0 341 34 A59 A59 NNP cord-319626-f1b3ygg0 341 35 virions virion NNS cord-319626-f1b3ygg0 341 36 and and CC cord-319626-f1b3ygg0 341 37 concommitantly concommitantly NN cord-319626-f1b3ygg0 341 38 blocks block VBZ cord-319626-f1b3ygg0 341 39 the the DT cord-319626-f1b3ygg0 341 40 completion completion NN cord-319626-f1b3ygg0 341 41 of of IN cord-319626-f1b3ygg0 341 42 the the DT cord-319626-f1b3ygg0 341 43 O o NN cord-319626-f1b3ygg0 341 44 - - HYPH cord-319626-f1b3ygg0 341 45 glycosylation glycosylation NN cord-319626-f1b3ygg0 341 46 of of IN cord-319626-f1b3ygg0 341 47 the the DT cord-319626-f1b3ygg0 341 48 E1 E1 NNP cord-319626-f1b3ygg0 341 49 viral viral JJ cord-319626-f1b3ygg0 341 50 envelope envelope NN cord-319626-f1b3ygg0 341 51 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 341 52 , , , cord-319626-f1b3ygg0 341 53 as as IN cord-319626-f1b3ygg0 341 54 we -PRON- PRP cord-319626-f1b3ygg0 341 55 have have VBP cord-319626-f1b3ygg0 341 56 shown show VBN cord-319626-f1b3ygg0 341 57 here here RB cord-319626-f1b3ygg0 341 58 . . . cord-319626-f1b3ygg0 342 1 Why why WRB cord-319626-f1b3ygg0 342 2 the the DT cord-319626-f1b3ygg0 342 3 transport transport NN cord-319626-f1b3ygg0 342 4 of of IN cord-319626-f1b3ygg0 342 5 MHV MHV NNP cord-319626-f1b3ygg0 342 6 - - HYPH cord-319626-f1b3ygg0 342 7 A59 A59 NNP cord-319626-f1b3ygg0 342 8 particles particle NNS cord-319626-f1b3ygg0 342 9 from from IN cord-319626-f1b3ygg0 342 10 the the DT cord-319626-f1b3ygg0 342 11 budding bud VBG cord-319626-f1b3ygg0 342 12 compartment compartment NN cord-319626-f1b3ygg0 342 13 to to IN cord-319626-f1b3ygg0 342 14 the the DT cord-319626-f1b3ygg0 342 15 Golgi Golgi NNP cord-319626-f1b3ygg0 342 16 apparatus apparatus NN cord-319626-f1b3ygg0 342 17 and and CC cord-319626-f1b3ygg0 342 18 thence thence RB cord-319626-f1b3ygg0 342 19 out out IN cord-319626-f1b3ygg0 342 20 of of IN cord-319626-f1b3ygg0 342 21 the the DT cord-319626-f1b3ygg0 342 22 cell cell NN cord-319626-f1b3ygg0 342 23 is be VBZ cord-319626-f1b3ygg0 342 24 so so RB cord-319626-f1b3ygg0 342 25 very very RB cord-319626-f1b3ygg0 342 26 much much RB cord-319626-f1b3ygg0 342 27 more more RBR cord-319626-f1b3ygg0 342 28 cold cold JJ cord-319626-f1b3ygg0 342 29 sensitive sensitive JJ cord-319626-f1b3ygg0 342 30 than than IN cord-319626-f1b3ygg0 342 31 the the DT cord-319626-f1b3ygg0 342 32 transport transport NN cord-319626-f1b3ygg0 342 33 out out IN cord-319626-f1b3ygg0 342 34 of of IN cord-319626-f1b3ygg0 342 35 the the DT cord-319626-f1b3ygg0 342 36 rough rough JJ cord-319626-f1b3ygg0 342 37 ER er NN cord-319626-f1b3ygg0 342 38 of of IN cord-319626-f1b3ygg0 342 39 the the DT cord-319626-f1b3ygg0 342 40 envelope envelope NN cord-319626-f1b3ygg0 342 41 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 342 42 of of IN cord-319626-f1b3ygg0 342 43 VSV VSV NNP cord-319626-f1b3ygg0 342 44 and and CC cord-319626-f1b3ygg0 342 45 SFV SFV NNP cord-319626-f1b3ygg0 342 46 remains remain VBZ cord-319626-f1b3ygg0 342 47 to to TO cord-319626-f1b3ygg0 342 48 be be VB cord-319626-f1b3ygg0 342 49 explained explain VBN cord-319626-f1b3ygg0 342 50 . . . cord-319626-f1b3ygg0 343 1 Possibly possibly RB cord-319626-f1b3ygg0 343 2 the the DT cord-319626-f1b3ygg0 343 3 difference difference NN cord-319626-f1b3ygg0 343 4 stems stem VBZ cord-319626-f1b3ygg0 343 5 from from IN cord-319626-f1b3ygg0 343 6 the the DT cord-319626-f1b3ygg0 343 7 fact fact NN cord-319626-f1b3ygg0 343 8 that that IN cord-319626-f1b3ygg0 343 9 in in IN cord-319626-f1b3ygg0 343 10 one one CD cord-319626-f1b3ygg0 343 11 case case NN cord-319626-f1b3ygg0 343 12 it -PRON- PRP cord-319626-f1b3ygg0 343 13 is be VBZ cord-319626-f1b3ygg0 343 14 whole whole JJ cord-319626-f1b3ygg0 343 15 virions virion NNS cord-319626-f1b3ygg0 343 16 which which WDT cord-319626-f1b3ygg0 343 17 are be VBP cord-319626-f1b3ygg0 343 18 being be VBG cord-319626-f1b3ygg0 343 19 transported transport VBN cord-319626-f1b3ygg0 343 20 and and CC cord-319626-f1b3ygg0 343 21 , , , cord-319626-f1b3ygg0 343 22 in in IN cord-319626-f1b3ygg0 343 23 the the DT cord-319626-f1b3ygg0 343 24 other other JJ cord-319626-f1b3ygg0 343 25 , , , cord-319626-f1b3ygg0 343 26 viral viral JJ cord-319626-f1b3ygg0 343 27 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 343 28 inserted insert VBN cord-319626-f1b3ygg0 343 29 into into IN cord-319626-f1b3ygg0 343 30 cellular cellular JJ cord-319626-f1b3ygg0 343 31 membranes membrane NNS cord-319626-f1b3ygg0 343 32 . . . cord-319626-f1b3ygg0 344 1 Sequence sequence NN cord-319626-f1b3ygg0 344 2 and and CC cord-319626-f1b3ygg0 344 3 topology topology NN cord-319626-f1b3ygg0 344 4 of of IN cord-319626-f1b3ygg0 344 5 a a DT cord-319626-f1b3ygg0 344 6 model model NN cord-319626-f1b3ygg0 344 7 intracellular intracellular JJ cord-319626-f1b3ygg0 344 8 membrane membrane NN cord-319626-f1b3ygg0 344 9 protein protein NN cord-319626-f1b3ygg0 344 10 , , , cord-319626-f1b3ygg0 344 11 E e NN cord-319626-f1b3ygg0 344 12 1 1 CD cord-319626-f1b3ygg0 344 13 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 344 14 , , , cord-319626-f1b3ygg0 344 15 from from IN cord-319626-f1b3ygg0 344 16 a a DT cord-319626-f1b3ygg0 344 17 coronavirus coronavirus NN cord-319626-f1b3ygg0 344 18 Structure structure NN cord-319626-f1b3ygg0 344 19 of of IN cord-319626-f1b3ygg0 344 20 the the DT cord-319626-f1b3ygg0 344 21 carbohydrate carbohydrate NN cord-319626-f1b3ygg0 344 22 units unit NNS cord-319626-f1b3ygg0 344 23 of of IN cord-319626-f1b3ygg0 344 24 lgA~ lga~ JJ cord-319626-f1b3ygg0 344 25 immunoglobulins immunoglobulin NNS cord-319626-f1b3ygg0 344 26 . . . cord-319626-f1b3ygg0 345 1 Structure structure NN cord-319626-f1b3ygg0 345 2 of of IN cord-319626-f1b3ygg0 345 3 the the DT cord-319626-f1b3ygg0 345 4 O o NN cord-319626-f1b3ygg0 345 5 - - HYPH cord-319626-f1b3ygg0 345 6 glycosidically glycosidically RB cord-319626-f1b3ygg0 345 7 linked link VBN cord-319626-f1b3ygg0 345 8 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 345 9 units unit NNS cord-319626-f1b3ygg0 345 10 Phase phase NN cord-319626-f1b3ygg0 345 11 separation separation NN cord-319626-f1b3ygg0 345 12 of of IN cord-319626-f1b3ygg0 345 13 integral integral JJ cord-319626-f1b3ygg0 345 14 membrane membrane NN cord-319626-f1b3ygg0 345 15 proteins protein NNS cord-319626-f1b3ygg0 345 16 in in IN cord-319626-f1b3ygg0 345 17 Triton Triton NNP cord-319626-f1b3ygg0 345 18 X X NNP cord-319626-f1b3ygg0 345 19 - - NNP cord-319626-f1b3ygg0 345 20 I14 I14 NNP cord-319626-f1b3ygg0 345 21 solution solution NN cord-319626-f1b3ygg0 345 22 Retention Retention NNP cord-319626-f1b3ygg0 345 23 of of IN cord-319626-f1b3ygg0 345 24 membrane membrane NN cord-319626-f1b3ygg0 345 25 proteins protein NNS cord-319626-f1b3ygg0 345 26 by by IN cord-319626-f1b3ygg0 345 27 the the DT cord-319626-f1b3ygg0 345 28 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 345 29 reticulum reticulum NN cord-319626-f1b3ygg0 345 30 A a DT cord-319626-f1b3ygg0 345 31 monoclonal monoclonal JJ cord-319626-f1b3ygg0 345 32 antibody antibody NN cord-319626-f1b3ygg0 345 33 against against IN cord-319626-f1b3ygg0 345 34 a a DT cord-319626-f1b3ygg0 345 35 135 135 CD cord-319626-f1b3ygg0 345 36 K K NNP cord-319626-f1b3ygg0 345 37 Golgi Golgi NNP cord-319626-f1b3ygg0 345 38 membrane membrane NN cord-319626-f1b3ygg0 345 39 protein protein NN cord-319626-f1b3ygg0 345 40 The the DT cord-319626-f1b3ygg0 345 41 sialic sialic JJ cord-319626-f1b3ygg0 345 42 acids acid NNS cord-319626-f1b3ygg0 345 43 . . . cord-319626-f1b3ygg0 346 1 XVI XVI NNP cord-319626-f1b3ygg0 346 2 . . . cord-319626-f1b3ygg0 347 1 Isolation isolation NN cord-319626-f1b3ygg0 347 2 of of IN cord-319626-f1b3ygg0 347 3 a a DT cord-319626-f1b3ygg0 347 4 mucin mucin NN cord-319626-f1b3ygg0 347 5 sialytransferase sialytransferase NN cord-319626-f1b3ygg0 347 6 from from IN cord-319626-f1b3ygg0 347 7 sheep sheep NN cord-319626-f1b3ygg0 347 8 submaxillary submaxillary NNP cord-319626-f1b3ygg0 347 9 glands gland NNS cord-319626-f1b3ygg0 347 10 Protein Protein NNP cord-319626-f1b3ygg0 347 11 synthesis synthesis NN cord-319626-f1b3ygg0 347 12 , , , cord-319626-f1b3ygg0 347 13 storage storage NN cord-319626-f1b3ygg0 347 14 , , , cord-319626-f1b3ygg0 347 15 and and CC cord-319626-f1b3ygg0 347 16 discharge discharge NN cord-319626-f1b3ygg0 347 17 in in IN cord-319626-f1b3ygg0 347 18 the the DT cord-319626-f1b3ygg0 347 19 pancreatic pancreatic JJ cord-319626-f1b3ygg0 347 20 exocrine exocrine JJ cord-319626-f1b3ygg0 347 21 cell cell NN cord-319626-f1b3ygg0 347 22 . . . cord-319626-f1b3ygg0 348 1 An an DT cord-319626-f1b3ygg0 348 2 autoradiographic autoradiographic JJ cord-319626-f1b3ygg0 348 3 study study NN cord-319626-f1b3ygg0 348 4 Biosynthesis Biosynthesis NNP cord-319626-f1b3ygg0 348 5 of of IN cord-319626-f1b3ygg0 348 6 N N NNP cord-319626-f1b3ygg0 348 7 - - : cord-319626-f1b3ygg0 348 8 and and CC cord-319626-f1b3ygg0 349 1 O o UH cord-319626-f1b3ygg0 349 2 - - HYPH cord-319626-f1b3ygg0 349 3 linked link VBN cord-319626-f1b3ygg0 349 4 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 349 5 of of IN cord-319626-f1b3ygg0 349 6 the the DT cord-319626-f1b3ygg0 349 7 low low JJ cord-319626-f1b3ygg0 349 8 density density NN cord-319626-f1b3ygg0 349 9 lipoprotein lipoprotein NN cord-319626-f1b3ygg0 349 10 receptor receptor NN cord-319626-f1b3ygg0 349 11 Assembly Assembly NNP cord-319626-f1b3ygg0 349 12 of of IN cord-319626-f1b3ygg0 349 13 enveloped envelop VBN cord-319626-f1b3ygg0 349 14 RNA RNA NNP cord-319626-f1b3ygg0 349 15 viruses virus NNS cord-319626-f1b3ygg0 349 16 Two two CD cord-319626-f1b3ygg0 349 17 enzymes enzyme NNS cord-319626-f1b3ygg0 349 18 involved involve VBN cord-319626-f1b3ygg0 349 19 in in IN cord-319626-f1b3ygg0 349 20 the the DT cord-319626-f1b3ygg0 349 21 synthesis synthesis NN cord-319626-f1b3ygg0 349 22 of of IN cord-319626-f1b3ygg0 349 23 O o NN cord-319626-f1b3ygg0 349 24 - - HYPH cord-319626-f1b3ygg0 349 25 linked link VBN cord-319626-f1b3ygg0 349 26 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 349 27 are be VBP cord-319626-f1b3ygg0 349 28 localized localize VBN cord-319626-f1b3ygg0 349 29 on on IN cord-319626-f1b3ygg0 349 30 membranes membrane NNS cord-319626-f1b3ygg0 349 31 of of IN cord-319626-f1b3ygg0 349 32 different different JJ cord-319626-f1b3ygg0 349 33 densities density NNS cord-319626-f1b3ygg0 349 34 in in IN cord-319626-f1b3ygg0 349 35 mouse mouse NN cord-319626-f1b3ygg0 349 36 lymphoma lymphoma NN cord-319626-f1b3ygg0 349 37 BW BW NNP cord-319626-f1b3ygg0 349 38 5147 5147 CD cord-319626-f1b3ygg0 349 39 cells cell NNS cord-319626-f1b3ygg0 350 1 Newly newly RB cord-319626-f1b3ygg0 350 2 synthesized synthesize VBN cord-319626-f1b3ygg0 350 3 G g NN cord-319626-f1b3ygg0 350 4 protein protein NN cord-319626-f1b3ygg0 350 5 of of IN cord-319626-f1b3ygg0 350 6 Vesicular Vesicular NNP cord-319626-f1b3ygg0 350 7 Stomatitis Stomatitis NNP cord-319626-f1b3ygg0 350 8 virus virus NN cord-319626-f1b3ygg0 350 9 is be VBZ cord-319626-f1b3ygg0 350 10 not not RB cord-319626-f1b3ygg0 350 11 transported transport VBN cord-319626-f1b3ygg0 350 12 to to IN cord-319626-f1b3ygg0 350 13 the the DT cord-319626-f1b3ygg0 350 14 Golgi Golgi NNP cord-319626-f1b3ygg0 350 15 complex complex NN cord-319626-f1b3ygg0 350 16 in in IN cord-319626-f1b3ygg0 350 17 mitotic mitotic JJ cord-319626-f1b3ygg0 350 18 cells cell NNS cord-319626-f1b3ygg0 351 1 An an DT cord-319626-f1b3ygg0 351 2 enzymatic enzymatic JJ cord-319626-f1b3ygg0 351 3 assay assay NN cord-319626-f1b3ygg0 351 4 reveals reveal VBZ cord-319626-f1b3ygg0 351 5 that that IN cord-319626-f1b3ygg0 351 6 proteins protein NNS cord-319626-f1b3ygg0 351 7 destined destine VBN cord-319626-f1b3ygg0 351 8 for for IN cord-319626-f1b3ygg0 351 9 the the DT cord-319626-f1b3ygg0 351 10 apical apical JJ cord-319626-f1b3ygg0 351 11 or or CC cord-319626-f1b3ygg0 351 12 basolateral basolateral JJ cord-319626-f1b3ygg0 351 13 domains domain NNS cord-319626-f1b3ygg0 351 14 of of IN cord-319626-f1b3ygg0 351 15 an an DT cord-319626-f1b3ygg0 351 16 epithelial epithelial JJ cord-319626-f1b3ygg0 351 17 cell cell NN cord-319626-f1b3ygg0 351 18 line line NN cord-319626-f1b3ygg0 351 19 share share NN cord-319626-f1b3ygg0 351 20 the the DT cord-319626-f1b3ygg0 351 21 same same JJ cord-319626-f1b3ygg0 351 22 late late JJ cord-319626-f1b3ygg0 351 23 Golgi Golgi NNP cord-319626-f1b3ygg0 351 24 compartments compartment NNS cord-319626-f1b3ygg0 351 25 Uukuniemi Uukuniemi NNP cord-319626-f1b3ygg0 351 26 virus virus NN cord-319626-f1b3ygg0 351 27 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 351 28 accumulate accumulate VBP cord-319626-f1b3ygg0 351 29 in in IN cord-319626-f1b3ygg0 351 30 and and CC cord-319626-f1b3ygg0 351 31 cause cause VB cord-319626-f1b3ygg0 351 32 morphological morphological JJ cord-319626-f1b3ygg0 351 33 changes change NNS cord-319626-f1b3ygg0 351 34 of of IN cord-319626-f1b3ygg0 351 35 the the DT cord-319626-f1b3ygg0 351 36 Golgi Golgi NNP cord-319626-f1b3ygg0 351 37 complex complex NN cord-319626-f1b3ygg0 351 38 in in IN cord-319626-f1b3ygg0 351 39 the the DT cord-319626-f1b3ygg0 351 40 absence absence NN cord-319626-f1b3ygg0 351 41 of of IN cord-319626-f1b3ygg0 351 42 Virus Virus NNP cord-319626-f1b3ygg0 351 43 maturation maturation NN cord-319626-f1b3ygg0 351 44 Passage passage NN cord-319626-f1b3ygg0 351 45 of of IN cord-319626-f1b3ygg0 351 46 viral viral JJ cord-319626-f1b3ygg0 351 47 membrane membrane NN cord-319626-f1b3ygg0 351 48 proteins protein NNS cord-319626-f1b3ygg0 351 49 through through IN cord-319626-f1b3ygg0 351 50 the the DT cord-319626-f1b3ygg0 351 51 Golgi Golgi NNP cord-319626-f1b3ygg0 351 52 complex complex NN cord-319626-f1b3ygg0 352 1 The the DT cord-319626-f1b3ygg0 352 2 trans trans NNP cord-319626-f1b3ygg0 352 3 Golgi Golgi NNP cord-319626-f1b3ygg0 352 4 network network NN cord-319626-f1b3ygg0 352 5 : : : cord-319626-f1b3ygg0 353 1 sorting sort VBG cord-319626-f1b3ygg0 353 2 at at IN cord-319626-f1b3ygg0 353 3 the the DT cord-319626-f1b3ygg0 353 4 exit exit NN cord-319626-f1b3ygg0 353 5 site site NN cord-319626-f1b3ygg0 353 6 of of IN cord-319626-f1b3ygg0 353 7 the the DT cord-319626-f1b3ygg0 353 8 Golgi Golgi NNP cord-319626-f1b3ygg0 353 9 complex complex JJ cord-319626-f1b3ygg0 353 10 Synthesis Synthesis NNP cord-319626-f1b3ygg0 353 11 of of IN cord-319626-f1b3ygg0 353 12 N N NNP cord-319626-f1b3ygg0 353 13 - - : cord-319626-f1b3ygg0 353 14 and and CC cord-319626-f1b3ygg0 353 15 O o NN cord-319626-f1b3ygg0 353 16 - - HYPH cord-319626-f1b3ygg0 353 17 linked link VBN cord-319626-f1b3ygg0 353 18 glycopeptides glycopeptide NNS cord-319626-f1b3ygg0 353 19 in in IN cord-319626-f1b3ygg0 353 20 oviduct oviduct NN cord-319626-f1b3ygg0 353 21 membrane membrane NN cord-319626-f1b3ygg0 353 22 preparations preparation NNS cord-319626-f1b3ygg0 353 23 Temporal temporal JJ cord-319626-f1b3ygg0 353 24 aspects aspect NNS cord-319626-f1b3ygg0 353 25 of of IN cord-319626-f1b3ygg0 353 26 the the DT cord-319626-f1b3ygg0 353 27 N N NNP cord-319626-f1b3ygg0 353 28 - - : cord-319626-f1b3ygg0 353 29 and and CC cord-319626-f1b3ygg0 353 30 O o NN cord-319626-f1b3ygg0 353 31 - - HYPH cord-319626-f1b3ygg0 353 32 linked link VBN cord-319626-f1b3ygg0 353 33 glycosylation glycosylation NN cord-319626-f1b3ygg0 353 34 of of IN cord-319626-f1b3ygg0 353 35 human human JJ cord-319626-f1b3ygg0 353 36 chorionic chorionic JJ cord-319626-f1b3ygg0 353 37 gonadotropin gonadotropin NN cord-319626-f1b3ygg0 353 38 Evolution evolution NN cord-319626-f1b3ygg0 353 39 ofa ofa NN cord-319626-f1b3ygg0 353 40 coronavirus coronavirus NN cord-319626-f1b3ygg0 353 41 during during IN cord-319626-f1b3ygg0 353 42 persistent persistent JJ cord-319626-f1b3ygg0 353 43 infection infection NN cord-319626-f1b3ygg0 353 44 in in IN cord-319626-f1b3ygg0 353 45 vitro vitro FW cord-319626-f1b3ygg0 353 46 Analysis Analysis NNP cord-319626-f1b3ygg0 353 47 of of IN cord-319626-f1b3ygg0 353 48 the the DT cord-319626-f1b3ygg0 353 49 functions function NNS cord-319626-f1b3ygg0 353 50 of of IN cord-319626-f1b3ygg0 353 51 coronavirus coronavirus NN cord-319626-f1b3ygg0 353 52 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 353 53 by by IN cord-319626-f1b3ygg0 353 54 differential differential JJ cord-319626-f1b3ygg0 353 55 inhibition inhibition NN cord-319626-f1b3ygg0 353 56 of of IN cord-319626-f1b3ygg0 353 57 synthesis synthesis NN cord-319626-f1b3ygg0 353 58 with with IN cord-319626-f1b3ygg0 353 59 tunicamycin tunicamycin NNP cord-319626-f1b3ygg0 354 1 Tunicamycin Tunicamycin NNP cord-319626-f1b3ygg0 354 2 resistant resistant JJ cord-319626-f1b3ygg0 354 3 glycosylation glycosylation NN cord-319626-f1b3ygg0 354 4 of of IN cord-319626-f1b3ygg0 354 5 a a DT cord-319626-f1b3ygg0 354 6 coronavirus coronavirus NN cord-319626-f1b3ygg0 354 7 glycoprntein glycoprntein NNP cord-319626-f1b3ygg0 354 8 : : : cord-319626-f1b3ygg0 354 9 demonstration demonstration NN cord-319626-f1b3ygg0 354 10 of of IN cord-319626-f1b3ygg0 354 11 a a DT cord-319626-f1b3ygg0 354 12 novel novel JJ cord-319626-f1b3ygg0 354 13 type type NN cord-319626-f1b3ygg0 354 14 of of IN cord-319626-f1b3ygg0 354 15 viral viral JJ cord-319626-f1b3ygg0 354 16 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 354 17 lntracellular lntracellular JJ cord-319626-f1b3ygg0 354 18 transport transport NN cord-319626-f1b3ygg0 354 19 of of IN cord-319626-f1b3ygg0 354 20 secretory secretory JJ cord-319626-f1b3ygg0 354 21 proteins protein NNS cord-319626-f1b3ygg0 354 22 in in IN cord-319626-f1b3ygg0 354 23 the the DT cord-319626-f1b3ygg0 354 24 pancreatic pancreatic JJ cord-319626-f1b3ygg0 354 25 exocrine exocrine JJ cord-319626-f1b3ygg0 354 26 cell cell NN cord-319626-f1b3ygg0 354 27 . . . cord-319626-f1b3ygg0 355 1 L L NNP cord-319626-f1b3ygg0 355 2 Role role NN cord-319626-f1b3ygg0 355 3 of of IN cord-319626-f1b3ygg0 355 4 the the DT cord-319626-f1b3ygg0 355 5 peripheral peripheral JJ cord-319626-f1b3ygg0 355 6 elements element NNS cord-319626-f1b3ygg0 355 7 of of IN cord-319626-f1b3ygg0 355 8 the the DT cord-319626-f1b3ygg0 355 9 Golgi Golgi NNP cord-319626-f1b3ygg0 355 10 complex complex JJ cord-319626-f1b3ygg0 355 11 O o NN cord-319626-f1b3ygg0 355 12 - - HYPH cord-319626-f1b3ygg0 355 13 linked link VBN cord-319626-f1b3ygg0 355 14 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 355 15 are be VBP cord-319626-f1b3ygg0 355 16 acquired acquire VBN cord-319626-f1b3ygg0 355 17 by by IN cord-319626-f1b3ygg0 355 18 herpes herpes NNP cord-319626-f1b3ygg0 355 19 simplex simplex NNP cord-319626-f1b3ygg0 355 20 virus virus NN cord-319626-f1b3ygg0 355 21 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 355 22 in in IN cord-319626-f1b3ygg0 355 23 the the DT cord-319626-f1b3ygg0 355 24 Golgi Golgi NNP cord-319626-f1b3ygg0 355 25 complex complex NN cord-319626-f1b3ygg0 355 26 Studies Studies NNPS cord-319626-f1b3ygg0 355 27 on on IN cord-319626-f1b3ygg0 355 28 Semliki Semliki NNP cord-319626-f1b3ygg0 355 29 forest forest NN cord-319626-f1b3ygg0 355 30 virus virus NN cord-319626-f1b3ygg0 355 31 subviral subviral JJ cord-319626-f1b3ygg0 355 32 components component NNS cord-319626-f1b3ygg0 355 33 Glycoprotein Glycoprotein NNP cord-319626-f1b3ygg0 355 34 biosynthesis biosynthesis NN cord-319626-f1b3ygg0 355 35 in in IN cord-319626-f1b3ygg0 355 36 the the DT cord-319626-f1b3ygg0 355 37 developing develop VBG cord-319626-f1b3ygg0 355 38 rat rat NN cord-319626-f1b3ygg0 355 39 brain brain NN cord-319626-f1b3ygg0 355 40 . . . cord-319626-f1b3ygg0 356 1 I. I. NNP cord-319626-f1b3ygg0 356 2 A a DT cord-319626-f1b3ygg0 356 3 study study NN cord-319626-f1b3ygg0 356 4 of of IN cord-319626-f1b3ygg0 356 5 glycotransferases glycotransferases NNP cord-319626-f1b3ygg0 356 6 in in IN cord-319626-f1b3ygg0 356 7 microsomal microsomal NNP cord-319626-f1b3ygg0 356 8 subfractions subfractions NNP cord-319626-f1b3ygg0 357 1 Glycoprotein glycoprotein NN cord-319626-f1b3ygg0 357 2 biosynthesis biosynthesis NN cord-319626-f1b3ygg0 357 3 in in IN cord-319626-f1b3ygg0 357 4 the the DT cord-319626-f1b3ygg0 357 5 developing develop VBG cord-319626-f1b3ygg0 357 6 rat rat NN cord-319626-f1b3ygg0 357 7 brain brain NN cord-319626-f1b3ygg0 357 8 . . . cord-319626-f1b3ygg0 358 1 II II NNP cord-319626-f1b3ygg0 358 2 . . . cord-319626-f1b3ygg0 359 1 Microsomal Microsomal NNP cord-319626-f1b3ygg0 359 2 galactosaminyltransferase galactosaminyltransferase NN cord-319626-f1b3ygg0 359 3 utilizing utilize VBG cord-319626-f1b3ygg0 359 4 endogenous endogenous JJ cord-319626-f1b3ygg0 359 5 and and CC cord-319626-f1b3ygg0 359 6 exogenous exogenous JJ cord-319626-f1b3ygg0 359 7 substrate substrate NN cord-319626-f1b3ygg0 359 8 acceptors acceptor NNS cord-319626-f1b3ygg0 359 9 Structure Structure NNP cord-319626-f1b3ygg0 359 10 of of IN cord-319626-f1b3ygg0 359 11 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 359 12 and and CC cord-319626-f1b3ygg0 359 13 their -PRON- PRP$ cord-319626-f1b3ygg0 359 14 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 359 15 units unit NNS cord-319626-f1b3ygg0 360 1 The the DT cord-319626-f1b3ygg0 360 2 synthesis synthesis NN cord-319626-f1b3ygg0 360 3 of of IN cord-319626-f1b3ygg0 360 4 complex complex JJ cord-319626-f1b3ygg0 360 5 - - HYPH cord-319626-f1b3ygg0 360 6 type type NN cord-319626-f1b3ygg0 360 7 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 360 8 . . . cord-319626-f1b3ygg0 361 1 I. I. NNP cord-319626-f1b3ygg0 361 2 Structure Structure NNP cord-319626-f1b3ygg0 361 3 of of IN cord-319626-f1b3ygg0 361 4 the the DT cord-319626-f1b3ygg0 361 5 lipid lipid NN cord-319626-f1b3ygg0 361 6 - - HYPH cord-319626-f1b3ygg0 361 7 linked link VBN cord-319626-f1b3ygg0 361 8 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 361 9 precursor precursor NN cord-319626-f1b3ygg0 361 10 of of IN cord-319626-f1b3ygg0 361 11 the the DT cord-319626-f1b3ygg0 361 12 complex complex JJ cord-319626-f1b3ygg0 361 13 - - HYPH cord-319626-f1b3ygg0 361 14 type type NN cord-319626-f1b3ygg0 361 15 oligosaccharide oligosaccharide NN cord-319626-f1b3ygg0 361 16 of of IN cord-319626-f1b3ygg0 361 17 the the DT cord-319626-f1b3ygg0 361 18 Vesicular Vesicular NNP cord-319626-f1b3ygg0 361 19 Stomatitis Stomatitis NNP cord-319626-f1b3ygg0 361 20 virus virus NN cord-319626-f1b3ygg0 361 21 G g NN cord-319626-f1b3ygg0 361 22 protein protein NN cord-319626-f1b3ygg0 361 23 Polyacrylamide Polyacrylamide NNP cord-319626-f1b3ygg0 361 24 gel gel NN cord-319626-f1b3ygg0 361 25 electrophoresis electrophoresis NN cord-319626-f1b3ygg0 361 26 of of IN cord-319626-f1b3ygg0 361 27 viral viral JJ cord-319626-f1b3ygg0 361 28 proteins protein NNS cord-319626-f1b3ygg0 361 29 Measurement Measurement NNP cord-319626-f1b3ygg0 361 30 of of IN cord-319626-f1b3ygg0 361 31 the the DT cord-319626-f1b3ygg0 361 32 incorporation incorporation NN cord-319626-f1b3ygg0 361 33 of of IN cord-319626-f1b3ygg0 361 34 radioactive radioactive JJ cord-319626-f1b3ygg0 361 35 amino amino NN cord-319626-f1b3ygg0 361 36 acids acid NNS cord-319626-f1b3ygg0 361 37 into into IN cord-319626-f1b3ygg0 361 38 proteins protein NNS cord-319626-f1b3ygg0 361 39 by by IN cord-319626-f1b3ygg0 361 40 a a DT cord-319626-f1b3ygg0 361 41 filter filter NN cord-319626-f1b3ygg0 361 42 - - HYPH cord-319626-f1b3ygg0 361 43 paper paper NN cord-319626-f1b3ygg0 361 44 disc disc NN cord-319626-f1b3ygg0 361 45 method method NN cord-319626-f1b3ygg0 361 46 Reduced reduce VBN cord-319626-f1b3ygg0 361 47 temperature temperature NN cord-319626-f1b3ygg0 361 48 prevents prevent VBZ cord-319626-f1b3ygg0 361 49 transfer transfer NN cord-319626-f1b3ygg0 361 50 of of IN cord-319626-f1b3ygg0 361 51 a a DT cord-319626-f1b3ygg0 361 52 membrane membrane NN cord-319626-f1b3ygg0 361 53 protein protein NN cord-319626-f1b3ygg0 361 54 to to IN cord-319626-f1b3ygg0 361 55 the the DT cord-319626-f1b3ygg0 361 56 cell cell NN cord-319626-f1b3ygg0 361 57 surface surface NN cord-319626-f1b3ygg0 361 58 but but CC cord-319626-f1b3ygg0 361 59 does do VBZ cord-319626-f1b3ygg0 361 60 not not RB cord-319626-f1b3ygg0 361 61 prevent prevent VB cord-319626-f1b3ygg0 361 62 terminal terminal JJ cord-319626-f1b3ygg0 361 63 glycosylation glycosylation NN cord-319626-f1b3ygg0 362 1 The the DT cord-319626-f1b3ygg0 362 2 reorganization reorganization NN cord-319626-f1b3ygg0 362 3 of of IN cord-319626-f1b3ygg0 362 4 the the DT cord-319626-f1b3ygg0 362 5 Golgi Golgi NNP cord-319626-f1b3ygg0 362 6 complex complex NN cord-319626-f1b3ygg0 362 7 in in IN cord-319626-f1b3ygg0 362 8 anoxic anoxic JJ cord-319626-f1b3ygg0 362 9 pancreatic pancreatic JJ cord-319626-f1b3ygg0 362 10 acinar acinar NN cord-319626-f1b3ygg0 362 11 ceils ceils NNP cord-319626-f1b3ygg0 362 12 Incorporation Incorporation NNP cord-319626-f1b3ygg0 362 13 of of IN cord-319626-f1b3ygg0 362 14 N N NNP cord-319626-f1b3ygg0 362 15 - - HYPH cord-319626-f1b3ygg0 362 16 acetylmannosamine acetylmannosamine NN cord-319626-f1b3ygg0 362 17 and and CC cord-319626-f1b3ygg0 362 18 N n CD cord-319626-f1b3ygg0 362 19 - - HYPH cord-319626-f1b3ygg0 362 20 acetylglucosamine acetylglucosamine NN cord-319626-f1b3ygg0 362 21 in in IN cord-319626-f1b3ygg0 362 22 rat rat NN cord-319626-f1b3ygg0 362 23 thyroid thyroid NN cord-319626-f1b3ygg0 362 24 in in IN cord-319626-f1b3ygg0 362 25 vitro vitro FW cord-319626-f1b3ygg0 362 26 Radiographic radiographic JJ cord-319626-f1b3ygg0 362 27 comparison comparison NN cord-319626-f1b3ygg0 362 28 of of IN cord-319626-f1b3ygg0 362 29 the the DT cord-319626-f1b3ygg0 362 30 uptake uptake NN cord-319626-f1b3ygg0 362 31 of of IN cord-319626-f1b3ygg0 362 32 galactose-[3H galactose-[3H NNP cord-319626-f1b3ygg0 362 33 ] ] -RRB- cord-319626-f1b3ygg0 362 34 and and CC cord-319626-f1b3ygg0 362 35 glucose-[3H glucose-[3h ADD cord-319626-f1b3ygg0 362 36 ] ] -RRB- cord-319626-f1b3ygg0 362 37 in in IN cord-319626-f1b3ygg0 362 38 the the DT cord-319626-f1b3ygg0 362 39 Golgi Golgi NNP cord-319626-f1b3ygg0 362 40 region region NN cord-319626-f1b3ygg0 362 41 of of IN cord-319626-f1b3ygg0 362 42 various various JJ cord-319626-f1b3ygg0 362 43 cells cell NNS cord-319626-f1b3ygg0 362 44 secreting secrete VBG cord-319626-f1b3ygg0 362 45 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 362 46 or or CC cord-319626-f1b3ygg0 362 47 mucopolysaccharides mucopolysaccharide VBZ cord-319626-f1b3ygg0 362 48 Coronavirus Coronavirus NNP cord-319626-f1b3ygg0 362 49 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 362 50 El El NNP cord-319626-f1b3ygg0 362 51 , , , cord-319626-f1b3ygg0 362 52 a a DT cord-319626-f1b3ygg0 362 53 new new JJ cord-319626-f1b3ygg0 362 54 type type NN cord-319626-f1b3ygg0 362 55 of of IN cord-319626-f1b3ygg0 362 56 viral viral JJ cord-319626-f1b3ygg0 362 57 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 362 58 Post post JJ cord-319626-f1b3ygg0 362 59 - - JJ cord-319626-f1b3ygg0 362 60 translational translational JJ cord-319626-f1b3ygg0 362 61 glycosylation glycosylation NN cord-319626-f1b3ygg0 362 62 of of IN cord-319626-f1b3ygg0 362 63 coronavirus coronavirus NN cord-319626-f1b3ygg0 362 64 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 362 65 El El NNP cord-319626-f1b3ygg0 362 66 : : : cord-319626-f1b3ygg0 362 67 inhibition inhibition NN cord-319626-f1b3ygg0 362 68 by by IN cord-319626-f1b3ygg0 362 69 monensin monensin NNP cord-319626-f1b3ygg0 363 1 The the DT cord-319626-f1b3ygg0 363 2 carbohydrates carbohydrate NNS cord-319626-f1b3ygg0 363 3 of of IN cord-319626-f1b3ygg0 363 4 MHV MHV NNP cord-319626-f1b3ygg0 363 5 - - HYPH cord-319626-f1b3ygg0 363 6 A59 A59 NNP cord-319626-f1b3ygg0 363 7 : : : cord-319626-f1b3ygg0 363 8 structures structure NNS cord-319626-f1b3ygg0 363 9 of of IN cord-319626-f1b3ygg0 363 10 the the DT cord-319626-f1b3ygg0 363 11 O o NN cord-319626-f1b3ygg0 363 12 - - HYPH cord-319626-f1b3ygg0 363 13 glycosidically glycosidically RB cord-319626-f1b3ygg0 363 14 linked link VBN cord-319626-f1b3ygg0 363 15 oligosaccharides oligosaccharide NNS cord-319626-f1b3ygg0 363 16 of of IN cord-319626-f1b3ygg0 363 17 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 363 18 El El NNP cord-319626-f1b3ygg0 363 19 A a DT cord-319626-f1b3ygg0 363 20 new new JJ cord-319626-f1b3ygg0 363 21 type type NN cord-319626-f1b3ygg0 363 22 of of IN cord-319626-f1b3ygg0 363 23 coated coat VBN cord-319626-f1b3ygg0 363 24 vesicular vesicular JJ cord-319626-f1b3ygg0 363 25 carrier carrier NN cord-319626-f1b3ygg0 363 26 that that WDT cord-319626-f1b3ygg0 363 27 appears appear VBZ cord-319626-f1b3ygg0 363 28 not not RB cord-319626-f1b3ygg0 363 29 to to TO cord-319626-f1b3ygg0 363 30 contain contain VB cord-319626-f1b3ygg0 363 31 clathrin clathrin NN cord-319626-f1b3ygg0 363 32 : : : cord-319626-f1b3ygg0 363 33 its -PRON- PRP$ cord-319626-f1b3ygg0 363 34 possible possible JJ cord-319626-f1b3ygg0 363 35 role role NN cord-319626-f1b3ygg0 363 36 in in IN cord-319626-f1b3ygg0 363 37 protein protein NN cord-319626-f1b3ygg0 363 38 transport transport NN cord-319626-f1b3ygg0 363 39 within within IN cord-319626-f1b3ygg0 363 40 the the DT cord-319626-f1b3ygg0 363 41 Goigi Goigi NNP cord-319626-f1b3ygg0 363 42 stack stack VB cord-319626-f1b3ygg0 363 43 Biosynthetic biosynthetic JJ cord-319626-f1b3ygg0 363 44 protein protein NN cord-319626-f1b3ygg0 363 45 transport transport NN cord-319626-f1b3ygg0 363 46 and and CC cord-319626-f1b3ygg0 363 47 sorting sorting NN cord-319626-f1b3ygg0 363 48 by by IN cord-319626-f1b3ygg0 363 49 the the DT cord-319626-f1b3ygg0 363 50 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 363 51 reticulum reticulum NN cord-319626-f1b3ygg0 363 52 Dissection dissection NN cord-319626-f1b3ygg0 363 53 of of IN cord-319626-f1b3ygg0 363 54 the the DT cord-319626-f1b3ygg0 363 55 Golgi Golgi NNP cord-319626-f1b3ygg0 363 56 complex complex NN cord-319626-f1b3ygg0 363 57 . . . cord-319626-f1b3ygg0 364 1 II II NNP cord-319626-f1b3ygg0 364 2 . . . cord-319626-f1b3ygg0 365 1 Density density NN cord-319626-f1b3ygg0 365 2 separation separation NN cord-319626-f1b3ygg0 365 3 of of IN cord-319626-f1b3ygg0 365 4 specific specific JJ cord-319626-f1b3ygg0 365 5 Golgi Golgi NNP cord-319626-f1b3ygg0 365 6 functions function NNS cord-319626-f1b3ygg0 365 7 in in IN cord-319626-f1b3ygg0 365 8 vitally vitally RB cord-319626-f1b3ygg0 365 9 infected infect VBN cord-319626-f1b3ygg0 365 10 cells cell NNS cord-319626-f1b3ygg0 365 11 treated treat VBN cord-319626-f1b3ygg0 365 12 with with IN cord-319626-f1b3ygg0 365 13 monensin monensin JJ cord-319626-f1b3ygg0 365 14 Enzymatic enzymatic JJ cord-319626-f1b3ygg0 365 15 characterization characterization NN cord-319626-f1b3ygg0 365 16 of of IN cord-319626-f1b3ygg0 365 17 a a DT cord-319626-f1b3ygg0 365 18 I~-v I~-v NNP cord-319626-f1b3ygg0 365 19 - - HYPH cord-319626-f1b3ygg0 365 20 galactoside galactoside NNP cord-319626-f1b3ygg0 365 21 ¢t2~3 ¢t2~3 CD cord-319626-f1b3ygg0 365 22 sialytransferase sialytransferase NN cord-319626-f1b3ygg0 365 23 from from IN cord-319626-f1b3ygg0 365 24 porcine porcine JJ cord-319626-f1b3ygg0 365 25 submaxillary submaxillary NN cord-319626-f1b3ygg0 365 26 glands gland NNS cord-319626-f1b3ygg0 365 27 Cytochemical Cytochemical NNP cord-319626-f1b3ygg0 365 28 localization localization NN cord-319626-f1b3ygg0 365 29 of of IN cord-319626-f1b3ygg0 365 30 terminal terminal JJ cord-319626-f1b3ygg0 365 31 N n CD cord-319626-f1b3ygg0 365 32 - - HYPH cord-319626-f1b3ygg0 365 33 acetylgalactosamine acetylgalactosamine JJ cord-319626-f1b3ygg0 365 34 residues residue NNS cord-319626-f1b3ygg0 365 35 in in IN cord-319626-f1b3ygg0 365 36 cellular cellular JJ cord-319626-f1b3ygg0 365 37 compartments compartment NNS cord-319626-f1b3ygg0 365 38 of of IN cord-319626-f1b3ygg0 365 39 intestinal intestinal JJ cord-319626-f1b3ygg0 365 40 goblet goblet NN cord-319626-f1b3ygg0 365 41 cells cell NNS cord-319626-f1b3ygg0 365 42 : : : cord-319626-f1b3ygg0 365 43 implications implication NNS cord-319626-f1b3ygg0 365 44 for for IN cord-319626-f1b3ygg0 365 45 the the DT cord-319626-f1b3ygg0 365 46 topology topology NN cord-319626-f1b3ygg0 365 47 of of IN cord-319626-f1b3ygg0 365 48 O o NN cord-319626-f1b3ygg0 365 49 - - HYPH cord-319626-f1b3ygg0 365 50 glycosylation glycosylation NN cord-319626-f1b3ygg0 366 1 The the DT cord-319626-f1b3ygg0 366 2 Golgi Golgi NNP cord-319626-f1b3ygg0 366 3 apparatus apparatus NN cord-319626-f1b3ygg0 366 4 : : : cord-319626-f1b3ygg0 366 5 two two CD cord-319626-f1b3ygg0 366 6 organelles organelle NNS cord-319626-f1b3ygg0 366 7 in in IN cord-319626-f1b3ygg0 366 8 tandem tandem JJ cord-319626-f1b3ygg0 366 9 Viral viral JJ cord-319626-f1b3ygg0 366 10 protein protein NN cord-319626-f1b3ygg0 366 11 synthesis synthesis NN cord-319626-f1b3ygg0 366 12 in in IN cord-319626-f1b3ygg0 366 13 mouse mouse NN cord-319626-f1b3ygg0 366 14 hepatitis hepatitis NNP cord-319626-f1b3ygg0 366 15 virus virus NN cord-319626-f1b3ygg0 366 16 - - HYPH cord-319626-f1b3ygg0 366 17 strain strain NN cord-319626-f1b3ygg0 366 18 A59 A59 NNP cord-319626-f1b3ygg0 366 19 infected infected JJ cord-319626-f1b3ygg0 366 20 cells cell NNS cord-319626-f1b3ygg0 366 21 : : : cord-319626-f1b3ygg0 366 22 effect effect NN cord-319626-f1b3ygg0 366 23 of of IN cord-319626-f1b3ygg0 366 24 tunicamycin tunicamycin NNS cord-319626-f1b3ygg0 366 25 Purification Purification NNP cord-319626-f1b3ygg0 366 26 to to IN cord-319626-f1b3ygg0 366 27 homogeneity homogeneity NN cord-319626-f1b3ygg0 366 28 and and CC cord-319626-f1b3ygg0 366 29 enzymatic enzymatic JJ cord-319626-f1b3ygg0 366 30 characterization characterization NN cord-319626-f1b3ygg0 366 31 of of IN cord-319626-f1b3ygg0 366 32 an an DT cord-319626-f1b3ygg0 366 33 a a DT cord-319626-f1b3ygg0 366 34 - - HYPH cord-319626-f1b3ygg0 366 35 N N NNP cord-319626-f1b3ygg0 366 36 - - HYPH cord-319626-f1b3ygg0 366 37 Acetylgalacostamine Acetylgalacostamine NNP cord-319626-f1b3ygg0 366 38 a a DT cord-319626-f1b3ygg0 366 39 2-"6 2-"6 CD cord-319626-f1b3ygg0 366 40 sialytransferase sialytransferase NN cord-319626-f1b3ygg0 366 41 from from IN cord-319626-f1b3ygg0 366 42 porcine porcine JJ cord-319626-f1b3ygg0 366 43 submaxillary submaxillary NN cord-319626-f1b3ygg0 366 44 glands gland NNS cord-319626-f1b3ygg0 366 45 Pre pre VB cord-319626-f1b3ygg0 366 46 - - : cord-319626-f1b3ygg0 366 47 and and CC cord-319626-f1b3ygg0 366 48 post post JJ cord-319626-f1b3ygg0 366 49 - - JJ cord-319626-f1b3ygg0 366 50 Golgi golgi JJ cord-319626-f1b3ygg0 366 51 vacuoles vacuole NNS cord-319626-f1b3ygg0 366 52 operate operate VBP cord-319626-f1b3ygg0 366 53 in in IN cord-319626-f1b3ygg0 366 54 the the DT cord-319626-f1b3ygg0 366 55 transport transport NN cord-319626-f1b3ygg0 366 56 of of IN cord-319626-f1b3ygg0 366 57 Semliki Semliki NNP cord-319626-f1b3ygg0 366 58 forest forest NN cord-319626-f1b3ygg0 366 59 virus virus NN cord-319626-f1b3ygg0 366 60 membrane membrane NN cord-319626-f1b3ygg0 366 61 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 366 62 to to IN cord-319626-f1b3ygg0 366 63 the the DT cord-319626-f1b3ygg0 366 64 cell cell NN cord-319626-f1b3ygg0 366 65 surface surface NN cord-319626-f1b3ygg0 366 66 Isolation isolation NN cord-319626-f1b3ygg0 366 67 and and CC cord-319626-f1b3ygg0 366 68 identification identification NN cord-319626-f1b3ygg0 366 69 of of IN cord-319626-f1b3ygg0 366 70 virus virus NN cord-319626-f1b3ygg0 366 71 - - HYPH cord-319626-f1b3ygg0 366 72 specific specific JJ cord-319626-f1b3ygg0 366 73 mRNA mrna NN cord-319626-f1b3ygg0 366 74 's 's POS cord-319626-f1b3ygg0 366 75 in in IN cord-319626-f1b3ygg0 366 76 cells cell NNS cord-319626-f1b3ygg0 366 77 infected infect VBN cord-319626-f1b3ygg0 366 78 with with IN cord-319626-f1b3ygg0 366 79 MHV MHV NNP cord-319626-f1b3ygg0 366 80 - - HYPH cord-319626-f1b3ygg0 366 81 A59 A59 NNP cord-319626-f1b3ygg0 366 82 Initial initial JJ cord-319626-f1b3ygg0 366 83 glycosylation glycosylation NN cord-319626-f1b3ygg0 366 84 of of IN cord-319626-f1b3ygg0 366 85 proteins protein NNS cord-319626-f1b3ygg0 366 86 with with IN cord-319626-f1b3ygg0 366 87 acetylgalactosaminyl acetylgalactosaminyl JJ cord-319626-f1b3ygg0 366 88 serine serine NN cord-319626-f1b3ygg0 366 89 linkages linkage NNS cord-319626-f1b3ygg0 367 1 The the DT cord-319626-f1b3ygg0 367 2 structure structure NN cord-319626-f1b3ygg0 367 3 and and CC cord-319626-f1b3ygg0 367 4 behavior behavior NN cord-319626-f1b3ygg0 367 5 of of IN cord-319626-f1b3ygg0 367 6 coronavirus coronavirus NN cord-319626-f1b3ygg0 367 7 A59 A59 NNP cord-319626-f1b3ygg0 367 8 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 368 1 The the DT cord-319626-f1b3ygg0 368 2 molecular molecular JJ cord-319626-f1b3ygg0 368 3 biology biology NN cord-319626-f1b3ygg0 368 4 of of IN cord-319626-f1b3ygg0 368 5 the the DT cord-319626-f1b3ygg0 368 6 coronaviruses coronaviruse NNS cord-319626-f1b3ygg0 369 1 The the DT cord-319626-f1b3ygg0 369 2 novel novel JJ cord-319626-f1b3ygg0 369 3 glycoproteins glycoprotein NNS cord-319626-f1b3ygg0 369 4 of of IN cord-319626-f1b3ygg0 369 5 coronaviruses coronaviruse NNS cord-319626-f1b3ygg0 369 6 Pertubation Pertubation NNP cord-319626-f1b3ygg0 369 7 of of IN cord-319626-f1b3ygg0 369 8 vesicular vesicular JJ cord-319626-f1b3ygg0 369 9 traffic traffic NN cord-319626-f1b3ygg0 369 10 with with IN cord-319626-f1b3ygg0 369 11 the the DT cord-319626-f1b3ygg0 369 12 carboxylic carboxylic NN cord-319626-f1b3ygg0 369 13 ionophore ionophore NNP cord-319626-f1b3ygg0 369 14 monensin monensin NNP cord-319626-f1b3ygg0 369 15 Temperature Temperature NNP cord-319626-f1b3ygg0 369 16 and and CC cord-319626-f1b3ygg0 369 17 energy energy NN cord-319626-f1b3ygg0 369 18 dependence dependence NN cord-319626-f1b3ygg0 369 19 of of IN cord-319626-f1b3ygg0 369 20 secretory secretory JJ cord-319626-f1b3ygg0 369 21 protein protein NN cord-319626-f1b3ygg0 369 22 transport transport NN cord-319626-f1b3ygg0 369 23 in in IN cord-319626-f1b3ygg0 369 24 the the DT cord-319626-f1b3ygg0 369 25 exocrine exocrine JJ cord-319626-f1b3ygg0 369 26 pancreas pancreas NN cord-319626-f1b3ygg0 369 27 Sorting sorting NN cord-319626-f1b3ygg0 369 28 of of IN cord-319626-f1b3ygg0 369 29 coronavirus coronavirus NN cord-319626-f1b3ygg0 369 30 from from IN cord-319626-f1b3ygg0 369 31 condensed condense VBN cord-319626-f1b3ygg0 369 32 secretory secretory JJ cord-319626-f1b3ygg0 369 33 proteins protein NNS cord-319626-f1b3ygg0 369 34 at at IN cord-319626-f1b3ygg0 369 35 the the DT cord-319626-f1b3ygg0 369 36 exit exit NN cord-319626-f1b3ygg0 369 37 from from IN cord-319626-f1b3ygg0 369 38 the the DT cord-319626-f1b3ygg0 369 39 trans trans NNP cord-319626-f1b3ygg0 369 40 Golgi Golgi NNP cord-319626-f1b3ygg0 369 41 network network NN cord-319626-f1b3ygg0 369 42 of of IN cord-319626-f1b3ygg0 369 43 AtT20 AtT20 NNP cord-319626-f1b3ygg0 369 44 cells cell NNS cord-319626-f1b3ygg0 369 45 Replication replication NN cord-319626-f1b3ygg0 369 46 of of IN cord-319626-f1b3ygg0 369 47 coronavirus coronavirus NN cord-319626-f1b3ygg0 369 48 MHV mhv NN cord-319626-f1b3ygg0 369 49 - - HYPH cord-319626-f1b3ygg0 369 50 A59 A59 NNP cord-319626-f1b3ygg0 369 51 in in IN cord-319626-f1b3ygg0 369 52 sac(- sac(- NNP cord-319626-f1b3ygg0 369 53 ) ) -RRB- cord-319626-f1b3ygg0 369 54 cells cell NNS cord-319626-f1b3ygg0 369 55 : : : cord-319626-f1b3ygg0 369 56 determination determination NN cord-319626-f1b3ygg0 369 57 of of IN cord-319626-f1b3ygg0 369 58 the the DT cord-319626-f1b3ygg0 369 59 first first JJ cord-319626-f1b3ygg0 369 60 site site NN cord-319626-f1b3ygg0 369 61 of of IN cord-319626-f1b3ygg0 369 62 budding budding NN cord-319626-f1b3ygg0 369 63 of of IN cord-319626-f1b3ygg0 369 64 progeny progeny JJ cord-319626-f1b3ygg0 369 65 virions virion NNS cord-319626-f1b3ygg0 369 66 laminated laminate VBN cord-319626-f1b3ygg0 369 67 cisteruae cisteruae NN cord-319626-f1b3ygg0 369 68 of of IN cord-319626-f1b3ygg0 369 69 the the DT cord-319626-f1b3ygg0 369 70 rough rough JJ cord-319626-f1b3ygg0 369 71 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 369 72 reticulum reticulum NN cord-319626-f1b3ygg0 369 73 induced induce VBN cord-319626-f1b3ygg0 369 74 by by IN cord-319626-f1b3ygg0 369 75 coronavirus coronavirus NN cord-319626-f1b3ygg0 369 76 MHV MHV NNP cord-319626-f1b3ygg0 369 77 - - HYPH cord-319626-f1b3ygg0 369 78 A59 A59 NNP cord-319626-f1b3ygg0 369 79 infection infection NN cord-319626-f1b3ygg0 369 80 Biogenesis biogenesis NN cord-319626-f1b3ygg0 369 81 of of IN cord-319626-f1b3ygg0 369 82 the the DT cord-319626-f1b3ygg0 369 83 E1 e1 NN cord-319626-f1b3ygg0 369 84 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 369 85 of of IN cord-319626-f1b3ygg0 369 86 MHV MHV NNP cord-319626-f1b3ygg0 369 87 - - HYPH cord-319626-f1b3ygg0 369 88 A59 A59 NNP cord-319626-f1b3ygg0 369 89 Identification Identification NNP cord-319626-f1b3ygg0 369 90 of of IN cord-319626-f1b3ygg0 369 91 two two CD cord-319626-f1b3ygg0 369 92 epitopes epitope NNS cord-319626-f1b3ygg0 369 93 in in IN cord-319626-f1b3ygg0 369 94 the the DT cord-319626-f1b3ygg0 369 95 carboxyterminal carboxyterminal JJ cord-319626-f1b3ygg0 369 96 15 15 CD cord-319626-f1b3ygg0 369 97 amino amino JJ cord-319626-f1b3ygg0 369 98 acids acid NNS cord-319626-f1b3ygg0 369 99 of of IN cord-319626-f1b3ygg0 369 100 the the DT cord-319626-f1b3ygg0 369 101 E1 e1 NN cord-319626-f1b3ygg0 369 102 glycoprotein glycoprotein NN cord-319626-f1b3ygg0 369 103 of of IN cord-319626-f1b3ygg0 369 104 mouse mouse NN cord-319626-f1b3ygg0 369 105 hepatitis hepatitis NN cord-319626-f1b3ygg0 369 106 virus virus NN cord-319626-f1b3ygg0 369 107 A59 A59 NNP cord-319626-f1b3ygg0 369 108 by by IN cord-319626-f1b3ygg0 369 109 using use VBG cord-319626-f1b3ygg0 369 110 hybrid hybrid JJ cord-319626-f1b3ygg0 369 111 proteins protein NNS cord-319626-f1b3ygg0 370 1 Is be VBZ cord-319626-f1b3ygg0 370 2 cytochrome cytochrome JJ cord-319626-f1b3ygg0 370 3 P-450 p-450 XX cord-319626-f1b3ygg0 370 4 transported transport VBN cord-319626-f1b3ygg0 370 5 from from IN cord-319626-f1b3ygg0 370 6 the the DT cord-319626-f1b3ygg0 370 7 endoplasmic endoplasmic NN cord-319626-f1b3ygg0 370 8 reticulum reticulum NN cord-319626-f1b3ygg0 370 9 to to IN cord-319626-f1b3ygg0 370 10 the the DT cord-319626-f1b3ygg0 370 11 Golgi Golgi NNP cord-319626-f1b3ygg0 370 12 apparatus apparatus NN cord-319626-f1b3ygg0 370 13 in in IN cord-319626-f1b3ygg0 370 14 rat rat NN cord-319626-f1b3ygg0 370 15 hepatocytes hepatocyte NNS cord-319626-f1b3ygg0 370 16 ? ? . cord-319626-f1b3ygg0 371 1 Received receive VBN cord-319626-f1b3ygg0 371 2 for for IN cord-319626-f1b3ygg0 371 3 publication publication NN cord-319626-f1b3ygg0 371 4 5 5 CD cord-319626-f1b3ygg0 371 5 October October NNP cord-319626-f1b3ygg0 371 6 1987 1987 CD cord-319626-f1b3ygg0 371 7 , , , cord-319626-f1b3ygg0 371 8 and and CC cord-319626-f1b3ygg0 371 9 in in IN cord-319626-f1b3ygg0 371 10 revised revise VBN cord-319626-f1b3ygg0 371 11 form form NN cord-319626-f1b3ygg0 371 12 6 6 CD cord-319626-f1b3ygg0 371 13 January January NNP cord-319626-f1b3ygg0 371 14 1988 1988 CD cord-319626-f1b3ygg0 371 15 . . .