id	sid	tid	token	lemma	pos
cord-299281-5z1xminb	1	1	key	key	JJ
cord-299281-5z1xminb	1	2	:	:	:
cord-299281-5z1xminb	1	3	cord-299281	cord-299281	NNP
cord-299281-5z1xminb	1	4	-	-	HYPH
cord-299281-5z1xminb	1	5	5z1xminb	5z1xminb	CD
cord-299281-5z1xminb	1	6	authors	author	NNS
cord-299281-5z1xminb	1	7	:	:	:
cord-299281-5z1xminb	2	1	nan	nan	NNP
cord-299281-5z1xminb	2	2	title	title	NN
cord-299281-5z1xminb	2	3	:	:	:
cord-299281-5z1xminb	3	1	Oligomerization	Oligomerization	NNP
cord-299281-5z1xminb	3	2	of	of	IN
cord-299281-5z1xminb	3	3	a	a	DT
cord-299281-5z1xminb	3	4	membrane	membrane	NN
cord-299281-5z1xminb	3	5	protein	protein	NN
cord-299281-5z1xminb	3	6	correlates	correlate	VBZ
cord-299281-5z1xminb	3	7	with	with	IN
cord-299281-5z1xminb	3	8	its	-PRON-	PRP$
cord-299281-5z1xminb	3	9	retention	retention	NN
cord-299281-5z1xminb	3	10	in	in	IN
cord-299281-5z1xminb	3	11	the	the	DT
cord-299281-5z1xminb	3	12	Golgi	Golgi	NNP
cord-299281-5z1xminb	3	13	complex	complex	JJ
cord-299281-5z1xminb	3	14	date	date	NN
cord-299281-5z1xminb	4	1	:	:	:
cord-299281-5z1xminb	4	2	1993	1993	CD
cord-299281-5z1xminb	4	3	-	-	SYM
cord-299281-5z1xminb	4	4	09	09	CD
cord-299281-5z1xminb	4	5	-	-	HYPH
cord-299281-5z1xminb	4	6	02	02	CD
cord-299281-5z1xminb	4	7	journal	journal	NN
cord-299281-5z1xminb	4	8	:	:	:
cord-299281-5z1xminb	5	1	J	J	NNP
cord-299281-5z1xminb	5	2	Cell	Cell	NNP
cord-299281-5z1xminb	5	3	Biol	Biol	NNP
cord-299281-5z1xminb	5	4	DOI	DOI	NNP
cord-299281-5z1xminb	5	5	:	:	:
cord-299281-5z1xminb	5	6	nan	nan	NNP
cord-299281-5z1xminb	5	7	sha	sha	NNP
cord-299281-5z1xminb	5	8	:	:	:
cord-299281-5z1xminb	5	9	1bfeba839c9c98eb6e49ceb13cfcdba28620a61d	1bfeba839c9c98eb6e49ceb13cfcdba28620a61d	NNP
cord-299281-5z1xminb	5	10	doc_id	doc_id	CD
cord-299281-5z1xminb	5	11	:	:	:
cord-299281-5z1xminb	6	1	299281	299281	CD
cord-299281-5z1xminb	6	2	cord_uid	cord_uid	NNS
cord-299281-5z1xminb	6	3	:	:	:
cord-299281-5z1xminb	6	4	5z1xminb	5z1xminb	CD
cord-299281-5z1xminb	7	1	The	the	DT
cord-299281-5z1xminb	7	2	first	first	JJ
cord-299281-5z1xminb	7	3	membrane	membrane	NN
cord-299281-5z1xminb	7	4	-	-	HYPH
cord-299281-5z1xminb	7	5	spanning	span	VBG
cord-299281-5z1xminb	7	6	domain	domain	NN
cord-299281-5z1xminb	7	7	(	(	-LRB-
cord-299281-5z1xminb	7	8	m1	m1	NNP
cord-299281-5z1xminb	7	9	)	)	-RRB-
cord-299281-5z1xminb	7	10	of	of	IN
cord-299281-5z1xminb	7	11	the	the	DT
cord-299281-5z1xminb	7	12	M	M	NNP
cord-299281-5z1xminb	7	13	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	7	14	of	of	IN
cord-299281-5z1xminb	7	15	avian	avian	JJ
cord-299281-5z1xminb	7	16	coronavirus	coronavirus	NN
cord-299281-5z1xminb	7	17	(	(	-LRB-
cord-299281-5z1xminb	7	18	formerly	formerly	RB
cord-299281-5z1xminb	7	19	called	call	VBN
cord-299281-5z1xminb	7	20	E1	E1	NNP
cord-299281-5z1xminb	7	21	)	)	-RRB-
cord-299281-5z1xminb	7	22	is	be	VBZ
cord-299281-5z1xminb	7	23	sufficient	sufficient	JJ
cord-299281-5z1xminb	7	24	to	to	TO
cord-299281-5z1xminb	7	25	retain	retain	VB
cord-299281-5z1xminb	7	26	this	this	DT
cord-299281-5z1xminb	7	27	protein	protein	NN
cord-299281-5z1xminb	7	28	in	in	IN
cord-299281-5z1xminb	7	29	the	the	DT
cord-299281-5z1xminb	7	30	cis	cis	NNP
cord-299281-5z1xminb	7	31	-	-	HYPH
cord-299281-5z1xminb	7	32	Golgi	Golgi	NNP
cord-299281-5z1xminb	7	33	.	.	.
cord-299281-5z1xminb	8	1	When	when	WRB
cord-299281-5z1xminb	8	2	the	the	DT
cord-299281-5z1xminb	8	3	membrane	membrane	NN
cord-299281-5z1xminb	8	4	-	-	HYPH
cord-299281-5z1xminb	8	5	spanning	span	VBG
cord-299281-5z1xminb	8	6	domain	domain	NN
cord-299281-5z1xminb	8	7	of	of	IN
cord-299281-5z1xminb	8	8	a	a	DT
cord-299281-5z1xminb	8	9	protein	protein	NN
cord-299281-5z1xminb	8	10	which	which	WDT
cord-299281-5z1xminb	8	11	is	be	VBZ
cord-299281-5z1xminb	8	12	efficiently	efficiently	RB
cord-299281-5z1xminb	8	13	delivered	deliver	VBN
cord-299281-5z1xminb	8	14	to	to	IN
cord-299281-5z1xminb	8	15	the	the	DT
cord-299281-5z1xminb	8	16	plasma	plasma	NN
cord-299281-5z1xminb	8	17	membrane	membrane	NN
cord-299281-5z1xminb	8	18	(	(	-LRB-
cord-299281-5z1xminb	8	19	VSV	VSV	NNP
cord-299281-5z1xminb	8	20	G	G	NNP
cord-299281-5z1xminb	8	21	protein	protein	NN
cord-299281-5z1xminb	8	22	)	)	-RRB-
cord-299281-5z1xminb	8	23	is	be	VBZ
cord-299281-5z1xminb	8	24	replaced	replace	VBN
cord-299281-5z1xminb	8	25	with	with	IN
cord-299281-5z1xminb	8	26	m1	m1	NNP
cord-299281-5z1xminb	8	27	,	,	,
cord-299281-5z1xminb	8	28	the	the	DT
cord-299281-5z1xminb	8	29	resulting	result	VBG
cord-299281-5z1xminb	8	30	chimera	chimera	NN
cord-299281-5z1xminb	8	31	(	(	-LRB-
cord-299281-5z1xminb	8	32	Gm1	gm1	NN
cord-299281-5z1xminb	8	33	)	)	-RRB-
cord-299281-5z1xminb	8	34	is	be	VBZ
cord-299281-5z1xminb	8	35	retained	retain	VBN
cord-299281-5z1xminb	8	36	in	in	IN
cord-299281-5z1xminb	8	37	the	the	DT
cord-299281-5z1xminb	8	38	Golgi	Golgi	NNP
cord-299281-5z1xminb	8	39	(	(	-LRB-
cord-299281-5z1xminb	8	40	Swift	Swift	NNP
cord-299281-5z1xminb	8	41	,	,	,
cord-299281-5z1xminb	8	42	A.	A.	NNP
cord-299281-5z1xminb	8	43	M.	M.	NNP
cord-299281-5z1xminb	8	44	,	,	,
cord-299281-5z1xminb	8	45	and	and	CC
cord-299281-5z1xminb	8	46	C.	C.	NNP
cord-299281-5z1xminb	8	47	E.	E.	NNP
cord-299281-5z1xminb	8	48	Machamer	Machamer	NNP
cord-299281-5z1xminb	8	49	.	.	.
cord-299281-5z1xminb	9	1	1991	1991	CD
cord-299281-5z1xminb	9	2	.	.	.
cord-299281-5z1xminb	10	1	J.	J.	NNP
cord-299281-5z1xminb	10	2	Cell	Cell	NNP
cord-299281-5z1xminb	10	3	Biol	Biol	NNP
cord-299281-5z1xminb	10	4	.	.	.
cord-299281-5z1xminb	11	1	115:19	115:19	CD
cord-299281-5z1xminb	11	2	-	-	SYM
cord-299281-5z1xminb	11	3	30	30	CD
cord-299281-5z1xminb	11	4	)	)	-RRB-
cord-299281-5z1xminb	11	5	.	.	.
cord-299281-5z1xminb	12	1	When	when	WRB
cord-299281-5z1xminb	12	2	assayed	assay	VBN
cord-299281-5z1xminb	12	3	in	in	IN
cord-299281-5z1xminb	12	4	sucrose	sucrose	NN
cord-299281-5z1xminb	12	5	gradients	gradient	NNS
cord-299281-5z1xminb	12	6	,	,	,
cord-299281-5z1xminb	12	7	we	-PRON-	PRP
cord-299281-5z1xminb	12	8	observed	observe	VBD
cord-299281-5z1xminb	12	9	that	that	IN
cord-299281-5z1xminb	12	10	Gm1	Gm1	NNP
cord-299281-5z1xminb	12	11	formed	form	VBD
cord-299281-5z1xminb	12	12	a	a	DT
cord-299281-5z1xminb	12	13	large	large	JJ
cord-299281-5z1xminb	12	14	oligomer	oligomer	NN
cord-299281-5z1xminb	12	15	,	,	,
cord-299281-5z1xminb	12	16	and	and	CC
cord-299281-5z1xminb	12	17	that	that	IN
cord-299281-5z1xminb	12	18	much	much	JJ
cord-299281-5z1xminb	12	19	of	of	IN
cord-299281-5z1xminb	12	20	this	this	DT
cord-299281-5z1xminb	12	21	oligomer	oligomer	NN
cord-299281-5z1xminb	12	22	was	be	VBD
cord-299281-5z1xminb	12	23	SDS	SDS	NNP
cord-299281-5z1xminb	12	24	resistant	resistant	JJ
cord-299281-5z1xminb	12	25	and	and	CC
cord-299281-5z1xminb	12	26	stayed	stay	VBD
cord-299281-5z1xminb	12	27	near	near	IN
cord-299281-5z1xminb	12	28	the	the	DT
cord-299281-5z1xminb	12	29	top	top	NN
cord-299281-5z1xminb	12	30	of	of	IN
cord-299281-5z1xminb	12	31	the	the	DT
cord-299281-5z1xminb	12	32	stacking	stacking	NN
cord-299281-5z1xminb	12	33	gel	gel	NN
cord-299281-5z1xminb	12	34	of	of	IN
cord-299281-5z1xminb	12	35	an	an	DT
cord-299281-5z1xminb	12	36	SDS	sds	NN
cord-299281-5z1xminb	12	37	-	-	HYPH
cord-299281-5z1xminb	12	38	polyacrylamide	polyacrylamide	NN
cord-299281-5z1xminb	12	39	gel	gel	NN
cord-299281-5z1xminb	12	40	.	.	.
cord-299281-5z1xminb	13	1	The	the	DT
cord-299281-5z1xminb	13	2	unusual	unusual	JJ
cord-299281-5z1xminb	13	3	stability	stability	NN
cord-299281-5z1xminb	13	4	of	of	IN
cord-299281-5z1xminb	13	5	the	the	DT
cord-299281-5z1xminb	13	6	oligomer	oligomer	NNP
cord-299281-5z1xminb	13	7	allowed	allow	VBD
cord-299281-5z1xminb	13	8	it	-PRON-	PRP
cord-299281-5z1xminb	13	9	to	to	TO
cord-299281-5z1xminb	13	10	be	be	VB
cord-299281-5z1xminb	13	11	detected	detect	VBN
cord-299281-5z1xminb	13	12	easily	easily	RB
cord-299281-5z1xminb	13	13	.	.	.
cord-299281-5z1xminb	14	1	Gm1	Gm1	NNP
cord-299281-5z1xminb	14	2	mutants	mutant	NNS
cord-299281-5z1xminb	14	3	with	with	IN
cord-299281-5z1xminb	14	4	single	single	JJ
cord-299281-5z1xminb	14	5	amino	amino	NN
cord-299281-5z1xminb	14	6	acid	acid	NN
cord-299281-5z1xminb	14	7	substitutions	substitution	NNS
cord-299281-5z1xminb	14	8	in	in	IN
cord-299281-5z1xminb	14	9	the	the	DT
cord-299281-5z1xminb	14	10	m1	m1	NNP
cord-299281-5z1xminb	14	11	domain	domain	NN
cord-299281-5z1xminb	14	12	that	that	WDT
cord-299281-5z1xminb	14	13	were	be	VBD
cord-299281-5z1xminb	14	14	retained	retain	VBN
cord-299281-5z1xminb	14	15	in	in	IN
cord-299281-5z1xminb	14	16	the	the	DT
cord-299281-5z1xminb	14	17	Golgi	Golgi	NNP
cord-299281-5z1xminb	14	18	complex	complex	NN
cord-299281-5z1xminb	14	19	formed	form	VBD
cord-299281-5z1xminb	14	20	SDS	sds	NN
cord-299281-5z1xminb	14	21	-	-	HYPH
cord-299281-5z1xminb	14	22	resistant	resistant	JJ
cord-299281-5z1xminb	14	23	oligomers	oligomer	NNS
cord-299281-5z1xminb	14	24	,	,	,
cord-299281-5z1xminb	14	25	whereas	whereas	IN
cord-299281-5z1xminb	14	26	mutants	mutant	NNS
cord-299281-5z1xminb	14	27	that	that	WDT
cord-299281-5z1xminb	14	28	were	be	VBD
cord-299281-5z1xminb	14	29	rapidly	rapidly	RB
cord-299281-5z1xminb	14	30	released	release	VBN
cord-299281-5z1xminb	14	31	to	to	IN
cord-299281-5z1xminb	14	32	the	the	DT
cord-299281-5z1xminb	14	33	plasma	plasma	NN
cord-299281-5z1xminb	14	34	membrane	membrane	NN
cord-299281-5z1xminb	14	35	did	do	VBD
cord-299281-5z1xminb	14	36	not	not	RB
cord-299281-5z1xminb	14	37	.	.	.
cord-299281-5z1xminb	15	1	Oligomerization	Oligomerization	NNP
cord-299281-5z1xminb	15	2	was	be	VBD
cord-299281-5z1xminb	15	3	not	not	RB
cord-299281-5z1xminb	15	4	detected	detect	VBN
cord-299281-5z1xminb	15	5	immediately	immediately	RB
cord-299281-5z1xminb	15	6	after	after	IN
cord-299281-5z1xminb	15	7	synthesis	synthesis	NN
cord-299281-5z1xminb	15	8	of	of	IN
cord-299281-5z1xminb	15	9	Gm1	gm1	NN
cord-299281-5z1xminb	15	10	,	,	,
cord-299281-5z1xminb	15	11	but	but	CC
cord-299281-5z1xminb	15	12	occurred	occur	VBD
cord-299281-5z1xminb	15	13	gradually	gradually	RB
cord-299281-5z1xminb	15	14	with	with	IN
cord-299281-5z1xminb	15	15	a	a	DT
cord-299281-5z1xminb	15	16	lag	lag	NN
cord-299281-5z1xminb	15	17	of	of	IN
cord-299281-5z1xminb	15	18	approximately	approximately	RB
cord-299281-5z1xminb	15	19	10	10	CD
cord-299281-5z1xminb	15	20	min	min	NN
cord-299281-5z1xminb	15	21	,	,	,
cord-299281-5z1xminb	15	22	suggesting	suggest	VBG
cord-299281-5z1xminb	15	23	that	that	IN
cord-299281-5z1xminb	15	24	it	-PRON-	PRP
cord-299281-5z1xminb	15	25	is	be	VBZ
cord-299281-5z1xminb	15	26	not	not	RB
cord-299281-5z1xminb	15	27	merely	merely	RB
cord-299281-5z1xminb	15	28	aggregation	aggregation	NN
cord-299281-5z1xminb	15	29	of	of	IN
cord-299281-5z1xminb	15	30	misfolded	misfolded	JJ
cord-299281-5z1xminb	15	31	proteins	protein	NNS
cord-299281-5z1xminb	15	32	.	.	.
cord-299281-5z1xminb	16	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	16	2	,	,	,
cord-299281-5z1xminb	16	3	oligomerization	oligomerization	NN
cord-299281-5z1xminb	16	4	did	do	VBD
cord-299281-5z1xminb	16	5	not	not	RB
cord-299281-5z1xminb	16	6	occur	occur	VB
cord-299281-5z1xminb	16	7	under	under	IN
cord-299281-5z1xminb	16	8	several	several	JJ
cord-299281-5z1xminb	16	9	conditions	condition	NNS
cord-299281-5z1xminb	16	10	that	that	WDT
cord-299281-5z1xminb	16	11	block	block	VBP
cord-299281-5z1xminb	16	12	ER	ER	NNP
cord-299281-5z1xminb	16	13	to	to	IN
cord-299281-5z1xminb	16	14	Golgi	Golgi	NNP
cord-299281-5z1xminb	16	15	transport	transport	NN
cord-299281-5z1xminb	16	16	.	.	.
cord-299281-5z1xminb	17	1	The	the	DT
cord-299281-5z1xminb	17	2	lumenal	lumenal	JJ
cord-299281-5z1xminb	17	3	domain	domain	NN
cord-299281-5z1xminb	17	4	was	be	VBD
cord-299281-5z1xminb	17	5	not	not	RB
cord-299281-5z1xminb	17	6	required	require	VBN
cord-299281-5z1xminb	17	7	for	for	IN
cord-299281-5z1xminb	17	8	oligomerization	oligomerization	NN
cord-299281-5z1xminb	17	9	since	since	IN
cord-299281-5z1xminb	17	10	another	another	DT
cord-299281-5z1xminb	17	11	chimera	chimera	NN
cord-299281-5z1xminb	17	12	(	(	-LRB-
cord-299281-5z1xminb	17	13	alpha	alpha	NN
cord-299281-5z1xminb	17	14	m1	m1	NNP
cord-299281-5z1xminb	17	15	G	G	NNP
cord-299281-5z1xminb	17	16	)	)	-RRB-
cord-299281-5z1xminb	17	17	,	,	,
cord-299281-5z1xminb	17	18	where	where	WRB
cord-299281-5z1xminb	17	19	the	the	DT
cord-299281-5z1xminb	17	20	lumenal	lumenal	JJ
cord-299281-5z1xminb	17	21	domain	domain	NN
cord-299281-5z1xminb	17	22	of	of	IN
cord-299281-5z1xminb	17	23	Gm1	gm1	NN
cord-299281-5z1xminb	17	24	was	be	VBD
cord-299281-5z1xminb	17	25	replaced	replace	VBN
cord-299281-5z1xminb	17	26	by	by	IN
cord-299281-5z1xminb	17	27	the	the	DT
cord-299281-5z1xminb	17	28	alpha	alpha	NN
cord-299281-5z1xminb	17	29	subunit	subunit	NN
cord-299281-5z1xminb	17	30	of	of	IN
cord-299281-5z1xminb	17	31	human	human	JJ
cord-299281-5z1xminb	17	32	chorionic	chorionic	JJ
cord-299281-5z1xminb	17	33	gonadotropin	gonadotropin	NN
cord-299281-5z1xminb	17	34	,	,	,
cord-299281-5z1xminb	17	35	also	also	RB
cord-299281-5z1xminb	17	36	formed	form	VBD
cord-299281-5z1xminb	17	37	an	an	DT
cord-299281-5z1xminb	17	38	SDS	SDS	NNP
cord-299281-5z1xminb	17	39	-	-	HYPH
cord-299281-5z1xminb	17	40	resistant	resistant	JJ
cord-299281-5z1xminb	17	41	oligomer	oligomer	NNP
cord-299281-5z1xminb	17	42	,	,	,
cord-299281-5z1xminb	17	43	and	and	CC
cord-299281-5z1xminb	17	44	was	be	VBD
cord-299281-5z1xminb	17	45	able	able	JJ
cord-299281-5z1xminb	17	46	to	to	TO
cord-299281-5z1xminb	17	47	form	form	VB
cord-299281-5z1xminb	17	48	hetero	hetero	NNS
cord-299281-5z1xminb	17	49	-	-	NNS
cord-299281-5z1xminb	17	50	oligomers	oligomer	NNS
cord-299281-5z1xminb	17	51	with	with	IN
cord-299281-5z1xminb	17	52	Gm1	gm1	NN
cord-299281-5z1xminb	17	53	as	as	IN
cord-299281-5z1xminb	17	54	revealed	reveal	VBN
cord-299281-5z1xminb	17	55	by	by	IN
cord-299281-5z1xminb	17	56	coprecipitation	coprecipitation	NN
cord-299281-5z1xminb	17	57	experiments	experiment	NNS
cord-299281-5z1xminb	17	58	.	.	.
cord-299281-5z1xminb	18	1	SDS	SDS	NNP
cord-299281-5z1xminb	18	2	resistance	resistance	NN
cord-299281-5z1xminb	18	3	was	be	VBD
cord-299281-5z1xminb	18	4	conferred	confer	VBN
cord-299281-5z1xminb	18	5	by	by	IN
cord-299281-5z1xminb	18	6	the	the	DT
cord-299281-5z1xminb	18	7	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	18	8	tail	tail	NN
cord-299281-5z1xminb	18	9	of	of	IN
cord-299281-5z1xminb	18	10	VSV	VSV	NNP
cord-299281-5z1xminb	18	11	G	G	NNP
cord-299281-5z1xminb	18	12	,	,	,
cord-299281-5z1xminb	18	13	because	because	IN
cord-299281-5z1xminb	18	14	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	18	15	digestion	digestion	NN
cord-299281-5z1xminb	18	16	of	of	IN
cord-299281-5z1xminb	18	17	the	the	DT
cord-299281-5z1xminb	18	18	tail	tail	NN
cord-299281-5z1xminb	18	19	in	in	IN
cord-299281-5z1xminb	18	20	microsomes	microsome	NNS
cord-299281-5z1xminb	18	21	containing	contain	VBG
cord-299281-5z1xminb	18	22	Gm1	Gm1	NNP
cord-299281-5z1xminb	18	23	oligomers	oligomer	NNS
cord-299281-5z1xminb	18	24	resulted	result	VBN
cord-299281-5z1xminb	18	25	in	in	IN
cord-299281-5z1xminb	18	26	loss	loss	NN
cord-299281-5z1xminb	18	27	of	of	IN
cord-299281-5z1xminb	18	28	SDS	SDS	NNP
cord-299281-5z1xminb	18	29	resistance	resistance	NN
cord-299281-5z1xminb	18	30	,	,	,
cord-299281-5z1xminb	18	31	although	although	IN
cord-299281-5z1xminb	18	32	the	the	DT
cord-299281-5z1xminb	18	33	protease	protease	NN
cord-299281-5z1xminb	18	34	-	-	HYPH
cord-299281-5z1xminb	18	35	treated	treat	VBN
cord-299281-5z1xminb	18	36	material	material	NN
cord-299281-5z1xminb	18	37	continued	continue	VBD
cord-299281-5z1xminb	18	38	to	to	TO
cord-299281-5z1xminb	18	39	migrate	migrate	VB
cord-299281-5z1xminb	18	40	as	as	IN
cord-299281-5z1xminb	18	41	a	a	DT
cord-299281-5z1xminb	18	42	large	large	JJ
cord-299281-5z1xminb	18	43	oligomer	oligomer	NN
cord-299281-5z1xminb	18	44	on	on	IN
cord-299281-5z1xminb	18	45	sucrose	sucrose	JJ
cord-299281-5z1xminb	18	46	gradients	gradient	NNS
cord-299281-5z1xminb	18	47	.	.	.
cord-299281-5z1xminb	19	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	19	2	,	,	,
cord-299281-5z1xminb	19	3	treatment	treatment	NN
cord-299281-5z1xminb	19	4	of	of	IN
cord-299281-5z1xminb	19	5	cells	cell	NNS
cord-299281-5z1xminb	19	6	with	with	IN
cord-299281-5z1xminb	19	7	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	19	8	D	D	NNP
cord-299281-5z1xminb	19	9	blocked	block	VBD
cord-299281-5z1xminb	19	10	formation	formation	NN
cord-299281-5z1xminb	19	11	of	of	IN
cord-299281-5z1xminb	19	12	SDS	sds	NN
cord-299281-5z1xminb	19	13	-	-	HYPH
cord-299281-5z1xminb	19	14	resistant	resistant	JJ
cord-299281-5z1xminb	19	15	(	(	-LRB-
cord-299281-5z1xminb	19	16	but	but	CC
cord-299281-5z1xminb	19	17	not	not	RB
cord-299281-5z1xminb	19	18	SDS-	sds-	JJ
cord-299281-5z1xminb	19	19	sensitive	sensitive	JJ
cord-299281-5z1xminb	19	20	)	)	-RRB-
cord-299281-5z1xminb	19	21	oligomers	oligomer	NNS
cord-299281-5z1xminb	19	22	.	.	.
cord-299281-5z1xminb	20	1	Our	-PRON-	PRP$
cord-299281-5z1xminb	20	2	data	datum	NNS
cord-299281-5z1xminb	20	3	suggest	suggest	VBP
cord-299281-5z1xminb	20	4	that	that	IN
cord-299281-5z1xminb	20	5	SDS	sds	NN
cord-299281-5z1xminb	20	6	-	-	HYPH
cord-299281-5z1xminb	20	7	resistant	resistant	JJ
cord-299281-5z1xminb	20	8	oligomers	oligomer	NNS
cord-299281-5z1xminb	20	9	form	form	VBP
cord-299281-5z1xminb	20	10	as	as	IN
cord-299281-5z1xminb	20	11	newly	newly	RB
cord-299281-5z1xminb	20	12	synthesized	synthesize	VBN
cord-299281-5z1xminb	20	13	molecules	molecule	NNS
cord-299281-5z1xminb	20	14	of	of	IN
cord-299281-5z1xminb	20	15	Gm1	gm1	NN
cord-299281-5z1xminb	20	16	arrive	arrive	VBP
cord-299281-5z1xminb	20	17	at	at	IN
cord-299281-5z1xminb	20	18	the	the	DT
cord-299281-5z1xminb	20	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	20	20	complex	complex	NN
cord-299281-5z1xminb	20	21	and	and	CC
cord-299281-5z1xminb	20	22	may	may	MD
cord-299281-5z1xminb	20	23	interact	interact	VB
cord-299281-5z1xminb	20	24	(	(	-LRB-
cord-299281-5z1xminb	20	25	directly	directly	RB
cord-299281-5z1xminb	20	26	or	or	CC
cord-299281-5z1xminb	20	27	indirectly	indirectly	RB
cord-299281-5z1xminb	20	28	)	)	-RRB-
cord-299281-5z1xminb	20	29	with	with	IN
cord-299281-5z1xminb	20	30	an	an	DT
cord-299281-5z1xminb	20	31	actin	actin	NN
cord-299281-5z1xminb	20	32	-	-	HYPH
cord-299281-5z1xminb	20	33	based	base	VBN
cord-299281-5z1xminb	20	34	cytoskeletal	cytoskeletal	JJ
cord-299281-5z1xminb	20	35	matrix	matrix	NN
cord-299281-5z1xminb	20	36	.	.	.
cord-299281-5z1xminb	21	1	The	the	DT
cord-299281-5z1xminb	21	2	oligomerization	oligomerization	NN
cord-299281-5z1xminb	21	3	of	of	IN
cord-299281-5z1xminb	21	4	Gm1	gm1	NN
cord-299281-5z1xminb	21	5	and	and	CC
cord-299281-5z1xminb	21	6	other	other	JJ
cord-299281-5z1xminb	21	7	resident	resident	NN
cord-299281-5z1xminb	21	8	proteins	protein	NNS
cord-299281-5z1xminb	21	9	could	could	MD
cord-299281-5z1xminb	21	10	serve	serve	VB
cord-299281-5z1xminb	21	11	as	as	IN
cord-299281-5z1xminb	21	12	a	a	DT
cord-299281-5z1xminb	21	13	mechanism	mechanism	NN
cord-299281-5z1xminb	21	14	for	for	IN
cord-299281-5z1xminb	21	15	their	-PRON-	PRP$
cord-299281-5z1xminb	21	16	retention	retention	NN
cord-299281-5z1xminb	21	17	in	in	IN
cord-299281-5z1xminb	21	18	the	the	DT
cord-299281-5z1xminb	21	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	21	20	complex	complex	NN
cord-299281-5z1xminb	21	21	.	.	.
cord-299281-5z1xminb	22	1	ization	ization	NN
cord-299281-5z1xminb	22	2	did	do	VBD
cord-299281-5z1xminb	22	3	not	not	RB
cord-299281-5z1xminb	22	4	occur	occur	VB
cord-299281-5z1xminb	22	5	under	under	IN
cord-299281-5z1xminb	22	6	several	several	JJ
cord-299281-5z1xminb	22	7	conditions	condition	NNS
cord-299281-5z1xminb	22	8	that	that	WDT
cord-299281-5z1xminb	22	9	block	block	VBP
cord-299281-5z1xminb	22	10	ER	ER	NNP
cord-299281-5z1xminb	22	11	to	to	IN
cord-299281-5z1xminb	22	12	Golgi	Golgi	NNP
cord-299281-5z1xminb	22	13	transport	transport	NN
cord-299281-5z1xminb	22	14	.	.	.
cord-299281-5z1xminb	23	1	The	the	DT
cord-299281-5z1xminb	23	2	lumenal	lumenal	JJ
cord-299281-5z1xminb	23	3	domain	domain	NN
cord-299281-5z1xminb	23	4	was	be	VBD
cord-299281-5z1xminb	23	5	not	not	RB
cord-299281-5z1xminb	23	6	required	require	VBN
cord-299281-5z1xminb	23	7	for	for	IN
cord-299281-5z1xminb	23	8	oligomerization	oligomerization	NN
cord-299281-5z1xminb	23	9	since	since	IN
cord-299281-5z1xminb	23	10	another	another	DT
cord-299281-5z1xminb	23	11	chimera	chimera	NN
cord-299281-5z1xminb	23	12	(	(	-LRB-
cord-299281-5z1xminb	23	13	otmlG	otmlG	NNP
cord-299281-5z1xminb	23	14	)	)	-RRB-
cord-299281-5z1xminb	23	15	,	,	,
cord-299281-5z1xminb	23	16	where	where	WRB
cord-299281-5z1xminb	23	17	the	the	DT
cord-299281-5z1xminb	23	18	lumenal	lumenal	JJ
cord-299281-5z1xminb	23	19	domain	domain	NN
cord-299281-5z1xminb	23	20	of	of	IN
cord-299281-5z1xminb	23	21	Gml	Gml	NNP
cord-299281-5z1xminb	23	22	was	be	VBD
cord-299281-5z1xminb	23	23	replaced	replace	VBN
cord-299281-5z1xminb	23	24	by	by	IN
cord-299281-5z1xminb	23	25	the	the	DT
cord-299281-5z1xminb	23	26	o~	o~	CD
cord-299281-5z1xminb	23	27	subunit	subunit	NN
cord-299281-5z1xminb	23	28	of	of	IN
cord-299281-5z1xminb	23	29	human	human	JJ
cord-299281-5z1xminb	23	30	chorionic	chorionic	JJ
cord-299281-5z1xminb	23	31	gonadotropin	gonadotropin	NN
cord-299281-5z1xminb	23	32	,	,	,
cord-299281-5z1xminb	23	33	also	also	RB
cord-299281-5z1xminb	23	34	formed	form	VBD
cord-299281-5z1xminb	23	35	an	an	DT
cord-299281-5z1xminb	23	36	SDS	SDS	NNP
cord-299281-5z1xminb	23	37	-	-	HYPH
cord-299281-5z1xminb	23	38	resistant	resistant	JJ
cord-299281-5z1xminb	23	39	oligomer	oligomer	NNP
cord-299281-5z1xminb	23	40	,	,	,
cord-299281-5z1xminb	23	41	and	and	CC
cord-299281-5z1xminb	23	42	was	be	VBD
cord-299281-5z1xminb	23	43	able	able	JJ
cord-299281-5z1xminb	23	44	to	to	TO
cord-299281-5z1xminb	23	45	form	form	VB
cord-299281-5z1xminb	23	46	hetero	hetero	NNS
cord-299281-5z1xminb	23	47	-	-	NNS
cord-299281-5z1xminb	23	48	oligomers	oligomer	NNS
cord-299281-5z1xminb	23	49	with	with	IN
cord-299281-5z1xminb	23	50	Gml	Gml	NNP
cord-299281-5z1xminb	23	51	as	as	IN
cord-299281-5z1xminb	23	52	revealed	reveal	VBN
cord-299281-5z1xminb	23	53	by	by	IN
cord-299281-5z1xminb	23	54	coprecipitation	coprecipitation	NN
cord-299281-5z1xminb	23	55	experiments	experiment	NNS
cord-299281-5z1xminb	23	56	.	.	.
cord-299281-5z1xminb	24	1	SDS	SDS	NNP
cord-299281-5z1xminb	24	2	resistance	resistance	NN
cord-299281-5z1xminb	24	3	was	be	VBD
cord-299281-5z1xminb	24	4	conferred	confer	VBN
cord-299281-5z1xminb	24	5	by	by	IN
cord-299281-5z1xminb	24	6	the	the	DT
cord-299281-5z1xminb	24	7	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	24	8	tail	tail	NN
cord-299281-5z1xminb	24	9	of	of	IN
cord-299281-5z1xminb	24	10	VSV	VSV	NNP
cord-299281-5z1xminb	24	11	G	G	NNP
cord-299281-5z1xminb	24	12	,	,	,
cord-299281-5z1xminb	24	13	because	because	IN
cord-299281-5z1xminb	24	14	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	24	15	digestion	digestion	NN
cord-299281-5z1xminb	24	16	of	of	IN
cord-299281-5z1xminb	24	17	the	the	DT
cord-299281-5z1xminb	24	18	tail	tail	NN
cord-299281-5z1xminb	24	19	in	in	IN
cord-299281-5z1xminb	24	20	microsomes	microsome	NNS
cord-299281-5z1xminb	24	21	containing	contain	VBG
cord-299281-5z1xminb	24	22	Gml	Gml	NNP
cord-299281-5z1xminb	24	23	oligomers	oligomer	NNS
cord-299281-5z1xminb	24	24	resulted	result	VBD
cord-299281-5z1xminb	24	25	in	in	IN
cord-299281-5z1xminb	24	26	loss	loss	NN
cord-299281-5z1xminb	24	27	of	of	IN
cord-299281-5z1xminb	24	28	SDS	SDS	NNP
cord-299281-5z1xminb	24	29	resistance	resistance	NN
cord-299281-5z1xminb	24	30	,	,	,
cord-299281-5z1xminb	24	31	although	although	IN
cord-299281-5z1xminb	24	32	the	the	DT
cord-299281-5z1xminb	24	33	protease	protease	NN
cord-299281-5z1xminb	24	34	-	-	HYPH
cord-299281-5z1xminb	24	35	treated	treat	VBN
cord-299281-5z1xminb	24	36	material	material	NN
cord-299281-5z1xminb	24	37	continued	continue	VBD
cord-299281-5z1xminb	24	38	to	to	TO
cord-299281-5z1xminb	24	39	migrate	migrate	VB
cord-299281-5z1xminb	24	40	as	as	IN
cord-299281-5z1xminb	24	41	a	a	DT
cord-299281-5z1xminb	24	42	large	large	JJ
cord-299281-5z1xminb	24	43	oligomer	oligomer	NN
cord-299281-5z1xminb	24	44	on	on	IN
cord-299281-5z1xminb	24	45	sucrose	sucrose	JJ
cord-299281-5z1xminb	24	46	gradients	gradient	NNS
cord-299281-5z1xminb	24	47	.	.	.
cord-299281-5z1xminb	25	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	25	2	,	,	,
cord-299281-5z1xminb	25	3	treatment	treatment	NN
cord-299281-5z1xminb	25	4	of	of	IN
cord-299281-5z1xminb	25	5	cells	cell	NNS
cord-299281-5z1xminb	25	6	with	with	IN
cord-299281-5z1xminb	25	7	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	25	8	D	D	NNP
cord-299281-5z1xminb	25	9	blocked	block	VBD
cord-299281-5z1xminb	25	10	formation	formation	NN
cord-299281-5z1xminb	25	11	of	of	IN
cord-299281-5z1xminb	25	12	SDS	sds	NN
cord-299281-5z1xminb	25	13	-	-	HYPH
cord-299281-5z1xminb	25	14	resistant	resistant	JJ
cord-299281-5z1xminb	25	15	(	(	-LRB-
cord-299281-5z1xminb	25	16	but	but	CC
cord-299281-5z1xminb	25	17	not	not	RB
cord-299281-5z1xminb	25	18	SDS	sds	NN
cord-299281-5z1xminb	25	19	-	-	HYPH
cord-299281-5z1xminb	25	20	sensitive	sensitive	JJ
cord-299281-5z1xminb	25	21	)	)	-RRB-
cord-299281-5z1xminb	25	22	oligomers	oligomer	NNS
cord-299281-5z1xminb	25	23	.	.	.
cord-299281-5z1xminb	26	1	Our	-PRON-	PRP$
cord-299281-5z1xminb	26	2	data	datum	NNS
cord-299281-5z1xminb	26	3	suggest	suggest	VBP
cord-299281-5z1xminb	26	4	that	that	IN
cord-299281-5z1xminb	26	5	SDS	sds	NN
cord-299281-5z1xminb	26	6	-	-	HYPH
cord-299281-5z1xminb	26	7	resistant	resistant	JJ
cord-299281-5z1xminb	26	8	oligomers	oligomer	NNS
cord-299281-5z1xminb	26	9	form	form	VBP
cord-299281-5z1xminb	26	10	as	as	IN
cord-299281-5z1xminb	26	11	newly	newly	RB
cord-299281-5z1xminb	26	12	synthesized	synthesize	VBN
cord-299281-5z1xminb	26	13	molecules	molecule	NNS
cord-299281-5z1xminb	26	14	of	of	IN
cord-299281-5z1xminb	26	15	Gml	Gml	NNP
cord-299281-5z1xminb	26	16	arrive	arrive	VBP
cord-299281-5z1xminb	26	17	at	at	IN
cord-299281-5z1xminb	26	18	the	the	DT
cord-299281-5z1xminb	26	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	26	20	complex	complex	NN
cord-299281-5z1xminb	26	21	and	and	CC
cord-299281-5z1xminb	26	22	may	may	MD
cord-299281-5z1xminb	26	23	interact	interact	VB
cord-299281-5z1xminb	26	24	(	(	-LRB-
cord-299281-5z1xminb	26	25	directly	directly	RB
cord-299281-5z1xminb	26	26	or	or	CC
cord-299281-5z1xminb	26	27	indirectly	indirectly	RB
cord-299281-5z1xminb	26	28	)	)	-RRB-
cord-299281-5z1xminb	26	29	with	with	IN
cord-299281-5z1xminb	26	30	an	an	DT
cord-299281-5z1xminb	26	31	actinbased	actinbased	JJ
cord-299281-5z1xminb	26	32	cytoskeletal	cytoskeletal	JJ
cord-299281-5z1xminb	26	33	matrix	matrix	NN
cord-299281-5z1xminb	26	34	.	.	.
cord-299281-5z1xminb	27	1	The	the	DT
cord-299281-5z1xminb	27	2	oligomerization	oligomerization	NN
cord-299281-5z1xminb	27	3	of	of	IN
cord-299281-5z1xminb	27	4	Gml	Gml	NNP
cord-299281-5z1xminb	27	5	and	and	CC
cord-299281-5z1xminb	27	6	other	other	JJ
cord-299281-5z1xminb	27	7	resident	resident	NN
cord-299281-5z1xminb	27	8	proteins	protein	NNS
cord-299281-5z1xminb	27	9	could	could	MD
cord-299281-5z1xminb	27	10	serve	serve	VB
cord-299281-5z1xminb	27	11	as	as	IN
cord-299281-5z1xminb	27	12	a	a	DT
cord-299281-5z1xminb	27	13	mechanism	mechanism	NN
cord-299281-5z1xminb	27	14	for	for	IN
cord-299281-5z1xminb	27	15	their	-PRON-	PRP$
cord-299281-5z1xminb	27	16	retention	retention	NN
cord-299281-5z1xminb	27	17	in	in	IN
cord-299281-5z1xminb	27	18	the	the	DT
cord-299281-5z1xminb	27	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	27	20	complex	complex	NN
cord-299281-5z1xminb	27	21	.	.	.
cord-299281-5z1xminb	28	1	T	T	NNP
cord-299281-5z1xminb	28	2	HE	HE	NNP
cord-299281-5z1xminb	28	3	Golgi	Golgi	NNP
cord-299281-5z1xminb	28	4	complex	complex	NN
cord-299281-5z1xminb	28	5	is	be	VBZ
cord-299281-5z1xminb	28	6	the	the	DT
cord-299281-5z1xminb	28	7	site	site	NN
cord-299281-5z1xminb	28	8	of	of	IN
cord-299281-5z1xminb	28	9	oligosaccharide	oligosaccharide	NN
cord-299281-5z1xminb	28	10	processing	processing	NN
cord-299281-5z1xminb	28	11	and	and	CC
cord-299281-5z1xminb	28	12	sorting	sort	VBG
cord-299281-5z1xminb	28	13	for	for	IN
cord-299281-5z1xminb	28	14	proteins	protein	NNS
cord-299281-5z1xminb	28	15	that	that	WDT
cord-299281-5z1xminb	28	16	are	be	VBP
cord-299281-5z1xminb	28	17	destined	destine	VBN
cord-299281-5z1xminb	28	18	for	for	IN
cord-299281-5z1xminb	28	19	delivery	delivery	NN
cord-299281-5z1xminb	28	20	to	to	IN
cord-299281-5z1xminb	28	21	secretory	secretory	JJ
cord-299281-5z1xminb	28	22	granules	granule	NNS
cord-299281-5z1xminb	28	23	,	,	,
cord-299281-5z1xminb	28	24	lysosomes	lysosome	NNS
cord-299281-5z1xminb	28	25	,	,	,
cord-299281-5z1xminb	28	26	the	the	DT
cord-299281-5z1xminb	28	27	plasma	plasma	NN
cord-299281-5z1xminb	28	28	membrane	membrane	NN
cord-299281-5z1xminb	28	29	,	,	,
cord-299281-5z1xminb	28	30	and	and	CC
cord-299281-5z1xminb	28	31	the	the	DT
cord-299281-5z1xminb	28	32	extracellular	extracellular	JJ
cord-299281-5z1xminb	28	33	space	space	NN
cord-299281-5z1xminb	28	34	.	.	.
cord-299281-5z1xminb	29	1	The	the	DT
cord-299281-5z1xminb	29	2	Golgi	Golgi	NNP
cord-299281-5z1xminb	29	3	-	-	HYPH
cord-299281-5z1xminb	29	4	resident	resident	NN
cord-299281-5z1xminb	29	5	proteins	protein	NNS
cord-299281-5z1xminb	29	6	that	that	WDT
cord-299281-5z1xminb	29	7	perform	perform	VBP
cord-299281-5z1xminb	29	8	these	these	DT
cord-299281-5z1xminb	29	9	functions	function	NNS
cord-299281-5z1xminb	29	10	must	must	MD
cord-299281-5z1xminb	29	11	be	be	VB
cord-299281-5z1xminb	29	12	retained	retain	VBN
cord-299281-5z1xminb	29	13	in	in	IN
cord-299281-5z1xminb	29	14	the	the	DT
cord-299281-5z1xminb	29	15	Golgi	Golgi	NNP
cord-299281-5z1xminb	29	16	despite	despite	IN
cord-299281-5z1xminb	29	17	the	the	DT
cord-299281-5z1xminb	29	18	large	large	JJ
cord-299281-5z1xminb	29	19	amount	amount	NN
cord-299281-5z1xminb	29	20	of	of	IN
cord-299281-5z1xminb	29	21	lipid	lipid	NN
cord-299281-5z1xminb	29	22	and	and	CC
cord-299281-5z1xminb	29	23	protein	protein	NN
cord-299281-5z1xminb	29	24	traffic	traffic	NN
cord-299281-5z1xminb	29	25	through	through	IN
cord-299281-5z1xminb	29	26	this	this	DT
cord-299281-5z1xminb	29	27	organelle	organelle	NN
cord-299281-5z1xminb	29	28	.	.	.
cord-299281-5z1xminb	30	1	Retention	retention	NN
cord-299281-5z1xminb	30	2	of	of	IN
cord-299281-5z1xminb	30	3	these	these	DT
cord-299281-5z1xminb	30	4	proteins	protein	NNS
cord-299281-5z1xminb	30	5	is	be	VBZ
cord-299281-5z1xminb	30	6	thought	think	VBN
cord-299281-5z1xminb	30	7	to	to	TO
cord-299281-5z1xminb	30	8	involve	involve	VB
cord-299281-5z1xminb	30	9	recognition	recognition	NN
cord-299281-5z1xminb	30	10	of	of	IN
cord-299281-5z1xminb	30	11	positive	positive	JJ
cord-299281-5z1xminb	30	12	signals	signal	NNS
cord-299281-5z1xminb	30	13	that	that	WDT
cord-299281-5z1xminb	30	14	specify	specify	VBP
cord-299281-5z1xminb	30	15	Golgi	Golgi	NNP
cord-299281-5z1xminb	30	16	localization	localization	NN
cord-299281-5z1xminb	30	17	.	.	.
cord-299281-5z1xminb	31	1	A	a	DT
cord-299281-5z1xminb	31	2	useful	useful	JJ
cord-299281-5z1xminb	31	3	model	model	NN
cord-299281-5z1xminb	31	4	protein	protein	NN
cord-299281-5z1xminb	31	5	for	for	IN
cord-299281-5z1xminb	31	6	studying	study	VBG
cord-299281-5z1xminb	31	7	Golgi	Golgi	NNP
cord-299281-5z1xminb	31	8	retention	retention	NN
cord-299281-5z1xminb	31	9	signals	signal	NNS
cord-299281-5z1xminb	31	10	has	have	VBZ
cord-299281-5z1xminb	31	11	been	be	VBN
cord-299281-5z1xminb	31	12	the	the	DT
cord-299281-5z1xminb	31	13	M	M	NNP
cord-299281-5z1xminb	31	14	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	31	15	(	(	-LRB-
cord-299281-5z1xminb	31	16	formerly	formerly	RB
cord-299281-5z1xminb	31	17	called	call	VBN
cord-299281-5z1xminb	31	18	El	El	NNP
cord-299281-5z1xminb	31	19	)	)	-RRB-
cord-299281-5z1xminb	31	20	of	of	IN
cord-299281-5z1xminb	31	21	the	the	DT
cord-299281-5z1xminb	31	22	avian	avian	JJ
cord-299281-5z1xminb	31	23	coronavirus	coronavirus	NN
cord-299281-5z1xminb	31	24	infectious	infectious	JJ
cord-299281-5z1xminb	31	25	bronchitis	bronchitis	NN
cord-299281-5z1xminb	31	26	virus	virus	NN
cord-299281-5z1xminb	31	27	(	(	-LRB-
cord-299281-5z1xminb	31	28	IBV)k	IBV)k	NNP
cord-299281-5z1xminb	31	29	Coronaviruses	Coronaviruses	NNP
cord-299281-5z1xminb	31	30	bud	bud	NN
cord-299281-5z1xminb	31	31	into	into	IN
cord-299281-5z1xminb	31	32	the	the	DT
cord-299281-5z1xminb	31	33	intermediate	intermediate	JJ
cord-299281-5z1xminb	31	34	compartment	compartment	NN
cord-299281-5z1xminb	31	35	or	or	CC
cord-299281-5z1xminb	31	36	Golgi	Golgi	NNP
cord-299281-5z1xminb	31	37	complex	complex	NN
cord-299281-5z1xminb	31	38	of	of	IN
cord-299281-5z1xminb	31	39	mammalian	mammalian	JJ
cord-299281-5z1xminb	31	40	cells	cell	NNS
cord-299281-5z1xminb	31	41	(	(	-LRB-
cord-299281-5z1xminb	31	42	Griffiths	Griffiths	NNP
cord-299281-5z1xminb	31	43	and	and	CC
cord-299281-5z1xminb	31	44	Rottier	Rottier	NNP
cord-299281-5z1xminb	31	45	,	,	,
cord-299281-5z1xminb	31	46	Please	please	UH
cord-299281-5z1xminb	31	47	address	address	VB
cord-299281-5z1xminb	31	48	all	all	DT
cord-299281-5z1xminb	31	49	correspondence	correspondence	NN
cord-299281-5z1xminb	31	50	to	to	IN
cord-299281-5z1xminb	31	51	Dr.	Dr.	NNP
cord-299281-5z1xminb	31	52	Carolyn	Carolyn	NNP
cord-299281-5z1xminb	31	53	Machamer	Machamer	NNP
cord-299281-5z1xminb	31	54	,	,	,
cord-299281-5z1xminb	31	55	Department	Department	NNP
cord-299281-5z1xminb	31	56	of	of	IN
cord-299281-5z1xminb	31	57	Cell	Cell	NNP
cord-299281-5z1xminb	31	58	Biology	Biology	NNP
cord-299281-5z1xminb	31	59	and	and	CC
cord-299281-5z1xminb	31	60	Anatomy	Anatomy	NNP
cord-299281-5z1xminb	31	61	,	,	,
cord-299281-5z1xminb	31	62	The	the	DT
cord-299281-5z1xminb	31	63	Johns	Johns	NNP
cord-299281-5z1xminb	31	64	Hopkins	Hopkins	NNP
cord-299281-5z1xminb	31	65	School	School	NNP
cord-299281-5z1xminb	31	66	of	of	IN
cord-299281-5z1xminb	31	67	Medicine	Medicine	NNP
cord-299281-5z1xminb	31	68	,	,	,
cord-299281-5z1xminb	31	69	725	725	CD
cord-299281-5z1xminb	31	70	N.	N.	NNP
cord-299281-5z1xminb	31	71	Wolfe	Wolfe	NNP
cord-299281-5z1xminb	31	72	Street	Street	NNP
cord-299281-5z1xminb	31	73	,	,	,
cord-299281-5z1xminb	31	74	Baltimore	Baltimore	NNP
cord-299281-5z1xminb	31	75	,	,	,
cord-299281-5z1xminb	31	76	MD	MD	NNP
cord-299281-5z1xminb	31	77	21205	21205	CD
cord-299281-5z1xminb	31	78	.	.	.
cord-299281-5z1xminb	32	1	Dr.	Dr.	NNP
cord-299281-5z1xminb	32	2	Ann	Ann	NNP
cord-299281-5z1xminb	32	3	M.	M.	NNP
cord-299281-5z1xminb	32	4	Swifts	Swifts	NNP
cord-299281-5z1xminb	32	5	current	current	JJ
cord-299281-5z1xminb	32	6	address	address	NN
cord-299281-5z1xminb	32	7	is	be	VBZ
cord-299281-5z1xminb	32	8	Smithkline	Smithkline	NNP
cord-299281-5z1xminb	32	9	Beecham	Beecham	NNP
cord-299281-5z1xminb	32	10	Pharmaceuticals	Pharmaceuticals	NNP
cord-299281-5z1xminb	32	11	,	,	,
cord-299281-5z1xminb	32	12	Philadelphia	Philadelphia	NNP
cord-299281-5z1xminb	32	13	,	,	,
cord-299281-5z1xminb	32	14	PA	PA	NNP
cord-299281-5z1xminb	32	15	.	.	.
cord-299281-5z1xminb	32	16	1992	1992	CD
cord-299281-5z1xminb	32	17	)	)	-RRB-
cord-299281-5z1xminb	32	18	.	.	.
cord-299281-5z1xminb	33	1	When	when	WRB
cord-299281-5z1xminb	33	2	cDNA	cdna	NN
cord-299281-5z1xminb	33	3	encoding	encode	VBG
cord-299281-5z1xminb	33	4	the	the	DT
cord-299281-5z1xminb	33	5	M	M	NNP
cord-299281-5z1xminb	33	6	protein	protein	NN
cord-299281-5z1xminb	33	7	is	be	VBZ
cord-299281-5z1xminb	33	8	expressed	express	VBN
cord-299281-5z1xminb	33	9	in	in	IN
cord-299281-5z1xminb	33	10	animal	animal	NN
cord-299281-5z1xminb	33	11	cells	cell	NNS
cord-299281-5z1xminb	33	12	,	,	,
cord-299281-5z1xminb	33	13	the	the	DT
cord-299281-5z1xminb	33	14	protein	protein	NN
cord-299281-5z1xminb	33	15	is	be	VBZ
cord-299281-5z1xminb	33	16	targeted	target	VBN
cord-299281-5z1xminb	33	17	to	to	IN
cord-299281-5z1xminb	33	18	the	the	DT
cord-299281-5z1xminb	33	19	cis	cis	NNP
cord-299281-5z1xminb	33	20	-	-	HYPH
cord-299281-5z1xminb	33	21	Golgi	Golgi	NNP
cord-299281-5z1xminb	33	22	(	(	-LRB-
cord-299281-5z1xminb	33	23	Machamer	Machamer	NNP
cord-299281-5z1xminb	33	24	et	et	NNP
cord-299281-5z1xminb	33	25	al	al	NNP
cord-299281-5z1xminb	33	26	.	.	NNP
cord-299281-5z1xminb	33	27	,	,	,
cord-299281-5z1xminb	33	28	1990	1990	CD
cord-299281-5z1xminb	33	29	)	)	-RRB-
cord-299281-5z1xminb	33	30	.	.	.
cord-299281-5z1xminb	34	1	Structurally	structurally	RB
cord-299281-5z1xminb	34	2	,	,	,
cord-299281-5z1xminb	34	3	the	the	DT
cord-299281-5z1xminb	34	4	IBV	IBV	NNP
cord-299281-5z1xminb	34	5	M	M	NNP
cord-299281-5z1xminb	34	6	protein	protein	NN
cord-299281-5z1xminb	34	7	consists	consist	VBZ
cord-299281-5z1xminb	34	8	of	of	IN
cord-299281-5z1xminb	34	9	a	a	DT
cord-299281-5z1xminb	34	10	short	short	JJ
cord-299281-5z1xminb	34	11	glycosylated	glycosylated	JJ
cord-299281-5z1xminb	34	12	amino	amino	NN
cord-299281-5z1xminb	34	13	-	-	HYPH
cord-299281-5z1xminb	34	14	terminal	terminal	NN
cord-299281-5z1xminb	34	15	domain	domain	NN
cord-299281-5z1xminb	34	16	,	,	,
cord-299281-5z1xminb	34	17	three	three	CD
cord-299281-5z1xminb	34	18	membrane	membrane	NN
cord-299281-5z1xminb	34	19	-	-	HYPH
cord-299281-5z1xminb	34	20	spanning	span	VBG
cord-299281-5z1xminb	34	21	domains	domain	NNS
cord-299281-5z1xminb	34	22	,	,	,
cord-299281-5z1xminb	34	23	and	and	CC
cord-299281-5z1xminb	34	24	a	a	DT
cord-299281-5z1xminb	34	25	long	long	JJ
cord-299281-5z1xminb	34	26	carboxyterminal	carboxyterminal	JJ
cord-299281-5z1xminb	34	27	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	34	28	domain	domain	NN
cord-299281-5z1xminb	34	29	.	.	.
cord-299281-5z1xminb	35	1	The	the	DT
cord-299281-5z1xminb	35	2	first	first	JJ
cord-299281-5z1xminb	35	3	membrane	membrane	NN
cord-299281-5z1xminb	35	4	-	-	HYPH
cord-299281-5z1xminb	35	5	spanning	span	VBG
cord-299281-5z1xminb	35	6	domain	domain	NN
cord-299281-5z1xminb	35	7	(	(	-LRB-
cord-299281-5z1xminb	35	8	ml	ml	NN
cord-299281-5z1xminb	35	9	)	)	-RRB-
cord-299281-5z1xminb	35	10	of	of	IN
cord-299281-5z1xminb	35	11	the	the	DT
cord-299281-5z1xminb	35	12	M	M	NNP
cord-299281-5z1xminb	35	13	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	35	14	is	be	VBZ
cord-299281-5z1xminb	35	15	sufficient	sufficient	JJ
cord-299281-5z1xminb	35	16	to	to	TO
cord-299281-5z1xminb	35	17	retain	retain	VB
cord-299281-5z1xminb	35	18	this	this	DT
cord-299281-5z1xminb	35	19	protein	protein	NN
cord-299281-5z1xminb	35	20	in	in	IN
cord-299281-5z1xminb	35	21	the	the	DT
cord-299281-5z1xminb	35	22	Golgi	Golgi	NNP
cord-299281-5z1xminb	35	23	(	(	-LRB-
cord-299281-5z1xminb	35	24	Machamer	Machamer	NNP
cord-299281-5z1xminb	35	25	and	and	CC
cord-299281-5z1xminb	35	26	Rose	Rose	NNP
cord-299281-5z1xminb	35	27	,	,	,
cord-299281-5z1xminb	35	28	1987	1987	CD
cord-299281-5z1xminb	35	29	)	)	-RRB-
cord-299281-5z1xminb	35	30	.	.	.
cord-299281-5z1xminb	36	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	36	2	,	,	,
cord-299281-5z1xminb	36	3	the	the	DT
cord-299281-5z1xminb	36	4	ml	ml	NN
cord-299281-5z1xminb	36	5	domain	domain	NN
cord-299281-5z1xminb	36	6	can	can	MD
cord-299281-5z1xminb	36	7	confer	confer	VB
cord-299281-5z1xminb	36	8	Golgi	Golgi	NNP
cord-299281-5z1xminb	36	9	localization	localization	NN
cord-299281-5z1xminb	36	10	to	to	IN
cord-299281-5z1xminb	36	11	a	a	DT
cord-299281-5z1xminb	36	12	wellcharacterized	wellcharacterized	JJ
cord-299281-5z1xminb	36	13	plasma	plasma	NN
cord-299281-5z1xminb	36	14	membrane	membrane	NN
cord-299281-5z1xminb	36	15	protein	protein	NN
cord-299281-5z1xminb	36	16	(	(	-LRB-
cord-299281-5z1xminb	36	17	the	the	DT
cord-299281-5z1xminb	36	18	G	G	NNP
cord-299281-5z1xminb	36	19	protein	protein	NN
cord-299281-5z1xminb	36	20	of	of	IN
cord-299281-5z1xminb	36	21	vesicular	vesicular	JJ
cord-299281-5z1xminb	36	22	stomatitis	stomatitis	NN
cord-299281-5z1xminb	36	23	virus	virus	NN
cord-299281-5z1xminb	36	24	,	,	,
cord-299281-5z1xminb	36	25	or	or	CC
cord-299281-5z1xminb	36	26	VSV	VSV	NNP
cord-299281-5z1xminb	36	27	G	G	NNP
cord-299281-5z1xminb	36	28	)	)	-RRB-
cord-299281-5z1xminb	36	29	.	.	.
cord-299281-5z1xminb	37	1	VSV	VSV	NNP
cord-299281-5z1xminb	37	2	G	G	NNP
cord-299281-5z1xminb	37	3	is	be	VBZ
cord-299281-5z1xminb	37	4	a	a	DT
cord-299281-5z1xminb	37	5	type	type	NN
cord-299281-5z1xminb	37	6	I	-PRON-	PRP
cord-299281-5z1xminb	37	7	membrane	membrane	NN
cord-299281-5z1xminb	37	8	protein	protein	NN
cord-299281-5z1xminb	37	9	which	which	WDT
cord-299281-5z1xminb	37	10	is	be	VBZ
cord-299281-5z1xminb	37	11	cotranslationally	cotranslationally	RB
cord-299281-5z1xminb	37	12	inserted	insert	VBN
cord-299281-5z1xminb	37	13	into	into	IN
cord-299281-5z1xminb	37	14	the	the	DT
cord-299281-5z1xminb	37	15	ER	ER	NNP
cord-299281-5z1xminb	37	16	,	,	,
cord-299281-5z1xminb	37	17	where	where	WRB
cord-299281-5z1xminb	37	18	it	-PRON-	PRP
cord-299281-5z1xminb	37	19	forms	form	VBZ
cord-299281-5z1xminb	37	20	homotrimers	homotrimer	NNS
cord-299281-5z1xminb	37	21	.	.	.
cord-299281-5z1xminb	38	1	It	-PRON-	PRP
cord-299281-5z1xminb	38	2	is	be	VBZ
cord-299281-5z1xminb	38	3	then	then	RB
cord-299281-5z1xminb	38	4	rapidly	rapidly	RB
cord-299281-5z1xminb	38	5	transported	transport	VBN
cord-299281-5z1xminb	38	6	through	through	IN
cord-299281-5z1xminb	38	7	the	the	DT
cord-299281-5z1xminb	38	8	Golgi	Golgi	NNP
cord-299281-5z1xminb	38	9	complex	complex	NN
cord-299281-5z1xminb	38	10	(	(	-LRB-
cord-299281-5z1xminb	38	11	as	as	IN
cord-299281-5z1xminb	38	12	determined	determine	VBN
cord-299281-5z1xminb	38	13	by	by	IN
cord-299281-5z1xminb	38	14	the	the	DT
cord-299281-5z1xminb	38	15	kinetics	kinetic	NNS
cord-299281-5z1xminb	38	16	of	of	IN
cord-299281-5z1xminb	38	17	N	N	NNP
cord-299281-5z1xminb	38	18	-	-	HYPH
cord-299281-5z1xminb	38	19	linked	link	VBN
cord-299281-5z1xminb	38	20	oligosaccharide	oligosaccharide	NN
cord-299281-5z1xminb	38	21	processing	processing	NN
cord-299281-5z1xminb	38	22	)	)	-RRB-
cord-299281-5z1xminb	38	23	and	and	CC
cord-299281-5z1xminb	38	24	delivered	deliver	VBN
cord-299281-5z1xminb	38	25	to	to	IN
cord-299281-5z1xminb	38	26	the	the	DT
cord-299281-5z1xminb	38	27	plasma	plasma	NN
cord-299281-5z1xminb	38	28	membrane	membrane	NN
cord-299281-5z1xminb	38	29	(	(	-LRB-
cord-299281-5z1xminb	38	30	for	for	IN
cord-299281-5z1xminb	38	31	review	review	NN
cord-299281-5z1xminb	38	32	see	see	VBP
cord-299281-5z1xminb	38	33	Doms	Doms	NNP
cord-299281-5z1xminb	38	34	et	et	NNP
cord-299281-5z1xminb	38	35	al	al	NNP
cord-299281-5z1xminb	38	36	.	.	NNP
cord-299281-5z1xminb	38	37	,	,	,
cord-299281-5z1xminb	38	38	1993	1993	CD
cord-299281-5z1xminb	38	39	)	)	-RRB-
cord-299281-5z1xminb	38	40	.	.	.
cord-299281-5z1xminb	39	1	When	when	WRB
cord-299281-5z1xminb	39	2	the	the	DT
cord-299281-5z1xminb	39	3	membrane	membrane	NN
cord-299281-5z1xminb	39	4	-	-	HYPH
cord-299281-5z1xminb	39	5	spanning	span	VBG
cord-299281-5z1xminb	39	6	domain	domain	NN
cord-299281-5z1xminb	39	7	of	of	IN
cord-299281-5z1xminb	39	8	VSV	VSV	NNP
cord-299281-5z1xminb	39	9	G	G	NNP
cord-299281-5z1xminb	39	10	is	be	VBZ
cord-299281-5z1xminb	39	11	replaced	replace	VBN
cord-299281-5z1xminb	39	12	with	with	IN
cord-299281-5z1xminb	39	13	the	the	DT
cord-299281-5z1xminb	39	14	ml	ml	NN
cord-299281-5z1xminb	39	15	domain	domain	NN
cord-299281-5z1xminb	39	16	of	of	IN
cord-299281-5z1xminb	39	17	IBV	IBV	NNP
cord-299281-5z1xminb	39	18	M	M	NNP
cord-299281-5z1xminb	39	19	,	,	,
cord-299281-5z1xminb	39	20	the	the	DT
cord-299281-5z1xminb	39	21	resulting	result	VBG
cord-299281-5z1xminb	39	22	chimera	chimera	NN
cord-299281-5z1xminb	39	23	(	(	-LRB-
cord-299281-5z1xminb	39	24	Gml	Gml	NNP
cord-299281-5z1xminb	39	25	,	,	,
cord-299281-5z1xminb	39	26	see	see	VB
cord-299281-5z1xminb	39	27	Fig	Fig	NNP
cord-299281-5z1xminb	39	28	.	.	.
cord-299281-5z1xminb	40	1	1	1	LS
cord-299281-5z1xminb	40	2	)	)	-RRB-
cord-299281-5z1xminb	40	3	is	be	VBZ
cord-299281-5z1xminb	40	4	retained	retain	VBN
cord-299281-5z1xminb	40	5	in	in	IN
cord-299281-5z1xminb	40	6	the	the	DT
cord-299281-5z1xminb	40	7	Golgi	Golgi	NNP
cord-299281-5z1xminb	40	8	complex	complex	NN
cord-299281-5z1xminb	40	9	(	(	-LRB-
cord-299281-5z1xminb	40	10	Swift	Swift	NNP
cord-299281-5z1xminb	40	11	and	and	CC
cord-299281-5z1xminb	40	12	Machamer	Machamer	NNP
cord-299281-5z1xminb	40	13	,	,	,
cord-299281-5z1xminb	40	14	1991	1991	CD
cord-299281-5z1xminb	40	15	)	)	-RRB-
cord-299281-5z1xminb	40	16	.	.	.
cord-299281-5z1xminb	41	1	Extensive	extensive	JJ
cord-299281-5z1xminb	41	2	mutagenesis	mutagenesis	NN
cord-299281-5z1xminb	41	3	suggests	suggest	VBZ
cord-299281-5z1xminb	41	4	that	that	IN
cord-299281-5z1xminb	41	5	at	at	RB
cord-299281-5z1xminb	41	6	least	least	RBS
cord-299281-5z1xminb	41	7	four	four	CD
cord-299281-5z1xminb	41	8	specific	specific	JJ
cord-299281-5z1xminb	41	9	amino	amino	NN
cord-299281-5z1xminb	41	10	acids	acid	NNS
cord-299281-5z1xminb	41	11	in	in	IN
cord-299281-5z1xminb	41	12	the	the	DT
cord-299281-5z1xminb	41	13	ml	ml	NN
cord-299281-5z1xminb	41	14	domain	domain	NN
cord-299281-5z1xminb	41	15	are	be	VBP
cord-299281-5z1xminb	41	16	critical	critical	JJ
cord-299281-5z1xminb	41	17	for	for	IN
cord-299281-5z1xminb	41	18	Golgi	Golgi	NNP
cord-299281-5z1xminb	41	19	retention	retention	NN
cord-299281-5z1xminb	41	20	of	of	IN
cord-299281-5z1xminb	41	21	Gml	Gml	NNP
cord-299281-5z1xminb	41	22	:	:	:
cord-299281-5z1xminb	41	23	asparagine	asparagine	NN
cord-299281-5z1xminb	41	24	465	465	CD
cord-299281-5z1xminb	41	25	,	,	,
cord-299281-5z1xminb	41	26	threonines	threonine	NNS
cord-299281-5z1xminb	41	27	469	469	CD
cord-299281-5z1xminb	41	28	and	and	CC
cord-299281-5z1xminb	41	29	476	476	CD
cord-299281-5z1xminb	41	30	,	,	,
cord-299281-5z1xminb	41	31	and	and	CC
cord-299281-5z1xminb	41	32	glutamine	glutamine	NN
cord-299281-5z1xminb	41	33	480	480	CD
cord-299281-5z1xminb	42	1	(	(	-LRB-
cord-299281-5z1xminb	42	2	Machamer	Machamer	NNP
cord-299281-5z1xminb	42	3	et	et	NNP
cord-299281-5z1xminb	42	4	al	al	NNP
cord-299281-5z1xminb	42	5	.	.	NNP
cord-299281-5z1xminb	42	6	,	,	,
cord-299281-5z1xminb	42	7	1993	1993	CD
cord-299281-5z1xminb	42	8	)	)	-RRB-
cord-299281-5z1xminb	42	9	.	.	.
cord-299281-5z1xminb	43	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	43	2	,	,	,
cord-299281-5z1xminb	43	3	these	these	DT
cord-299281-5z1xminb	43	4	residues	residue	NNS
cord-299281-5z1xminb	43	5	form	form	VBP
cord-299281-5z1xminb	43	6	an	an	DT
cord-299281-5z1xminb	43	7	uncharged	uncharged	JJ
cord-299281-5z1xminb	43	8	polar	polar	JJ
cord-299281-5z1xminb	43	9	face	face	NN
cord-299281-5z1xminb	43	10	along	along	IN
cord-299281-5z1xminb	43	11	one	one	CD
cord-299281-5z1xminb	43	12	side	side	NN
cord-299281-5z1xminb	43	13	of	of	IN
cord-299281-5z1xminb	43	14	the	the	DT
cord-299281-5z1xminb	43	15	alpha	alpha	NN
cord-299281-5z1xminb	43	16	helix	helix	NN
cord-299281-5z1xminb	43	17	predicted	predict	VBD
cord-299281-5z1xminb	43	18	for	for	IN
cord-299281-5z1xminb	43	19	ml	ml	NN
cord-299281-5z1xminb	43	20	.	.	.
cord-299281-5z1xminb	44	1	The	the	DT
cord-299281-5z1xminb	44	2	polar	polar	JJ
cord-299281-5z1xminb	44	3	nature	nature	NN
cord-299281-5z1xminb	44	4	of	of	IN
cord-299281-5z1xminb	44	5	this	this	DT
cord-299281-5z1xminb	44	6	face	face	NN
cord-299281-5z1xminb	44	7	of	of	IN
cord-299281-5z1xminb	44	8	the	the	DT
cord-299281-5z1xminb	44	9	helix	helix	NN
cord-299281-5z1xminb	44	10	suggests	suggest	VBZ
cord-299281-5z1xminb	44	11	that	that	IN
cord-299281-5z1xminb	44	12	protein	protein	NN
cord-299281-5z1xminb	44	13	-	-	HYPH
cord-299281-5z1xminb	44	14	protein	protein	NN
cord-299281-5z1xminb	44	15	interactions	interaction	NNS
cord-299281-5z1xminb	44	16	along	along	IN
cord-299281-5z1xminb	44	17	this	this	DT
cord-299281-5z1xminb	44	18	face	face	NN
cord-299281-5z1xminb	44	19	may	may	MD
cord-299281-5z1xminb	44	20	mediate	mediate	VB
cord-299281-5z1xminb	44	21	Golgi	Golgi	NNP
cord-299281-5z1xminb	44	22	retention	retention	NN
cord-299281-5z1xminb	44	23	of	of	IN
cord-299281-5z1xminb	44	24	Gml	Gml	NNP
cord-299281-5z1xminb	44	25	.	.	.
cord-299281-5z1xminb	45	1	There	there	EX
cord-299281-5z1xminb	45	2	are	be	VBP
cord-299281-5z1xminb	45	3	two	two	CD
cord-299281-5z1xminb	45	4	general	general	JJ
cord-299281-5z1xminb	45	5	ways	way	NNS
cord-299281-5z1xminb	45	6	to	to	TO
cord-299281-5z1xminb	45	7	envision	envision	VB
cord-299281-5z1xminb	45	8	the	the	DT
cord-299281-5z1xminb	45	9	specific	specific	JJ
cord-299281-5z1xminb	45	10	retention	retention	NN
cord-299281-5z1xminb	45	11	of	of	IN
cord-299281-5z1xminb	45	12	a	a	DT
cord-299281-5z1xminb	45	13	protein	protein	NN
cord-299281-5z1xminb	45	14	by	by	IN
cord-299281-5z1xminb	45	15	information	information	NN
cord-299281-5z1xminb	45	16	contained	contain	VBN
cord-299281-5z1xminb	45	17	within	within	IN
cord-299281-5z1xminb	45	18	its	-PRON-	PRP$
cord-299281-5z1xminb	45	19	transmembrane	transmembrane	NN
cord-299281-5z1xminb	45	20	domain	domain	NN
cord-299281-5z1xminb	45	21	(	(	-LRB-
cord-299281-5z1xminb	45	22	for	for	IN
cord-299281-5z1xminb	45	23	review	review	NN
cord-299281-5z1xminb	45	24	see	see	VB
cord-299281-5z1xminb	45	25	Machamer	Machamer	NNP
cord-299281-5z1xminb	45	26	,	,	,
cord-299281-5z1xminb	45	27	1991	1991	CD
cord-299281-5z1xminb	45	28	)	)	-RRB-
cord-299281-5z1xminb	45	29	.	.	.
cord-299281-5z1xminb	46	1	The	the	DT
cord-299281-5z1xminb	46	2	first	first	JJ
cord-299281-5z1xminb	46	3	involves	involve	VBZ
cord-299281-5z1xminb	46	4	the	the	DT
cord-299281-5z1xminb	46	5	recognition	recognition	NN
cord-299281-5z1xminb	46	6	of	of	IN
cord-299281-5z1xminb	46	7	this	this	DT
cord-299281-5z1xminb	46	8	domain	domain	NN
cord-299281-5z1xminb	46	9	by	by	IN
cord-299281-5z1xminb	46	10	a	a	DT
cord-299281-5z1xminb	46	11	specific	specific	JJ
cord-299281-5z1xminb	46	12	receptor	receptor	NN
cord-299281-5z1xminb	46	13	that	that	WDT
cord-299281-5z1xminb	46	14	either	either	DT
cord-299281-5z1xminb	46	15	blocks	block	VBZ
cord-299281-5z1xminb	46	16	further	further	JJ
cord-299281-5z1xminb	46	17	movement	movement	NN
cord-299281-5z1xminb	46	18	in	in	IN
cord-299281-5z1xminb	46	19	the	the	DT
cord-299281-5z1xminb	46	20	exocytic	exocytic	JJ
cord-299281-5z1xminb	46	21	pathway	pathway	NN
cord-299281-5z1xminb	46	22	or	or	CC
cord-299281-5z1xminb	46	23	retrieves	retrieve	VBZ
cord-299281-5z1xminb	46	24	escaped	escape	VBD
cord-299281-5z1xminb	46	25	proteins	protein	NNS
cord-299281-5z1xminb	46	26	back	back	RB
cord-299281-5z1xminb	46	27	to	to	IN
cord-299281-5z1xminb	46	28	the	the	DT
cord-299281-5z1xminb	46	29	appropriate	appropriate	JJ
cord-299281-5z1xminb	46	30	compartment	compartment	NN
cord-299281-5z1xminb	46	31	.	.	.
cord-299281-5z1xminb	47	1	A	a	DT
cord-299281-5z1xminb	47	2	receptor	receptor	NN
cord-299281-5z1xminb	47	3	-	-	HYPH
cord-299281-5z1xminb	47	4	based	base	VBN
cord-299281-5z1xminb	47	5	model	model	NN
cord-299281-5z1xminb	47	6	also	also	RB
cord-299281-5z1xminb	47	7	requires	require	VBZ
cord-299281-5z1xminb	47	8	a	a	DT
cord-299281-5z1xminb	47	9	mechanism	mechanism	NN
cord-299281-5z1xminb	47	10	for	for	IN
cord-299281-5z1xminb	47	11	retention	retention	NN
cord-299281-5z1xminb	47	12	or	or	CC
cord-299281-5z1xminb	47	13	recycling	recycling	NN
cord-299281-5z1xminb	47	14	of	of	IN
cord-299281-5z1xminb	47	15	the	the	DT
cord-299281-5z1xminb	47	16	receptor	receptor	NN
cord-299281-5z1xminb	47	17	itself	-PRON-	PRP
cord-299281-5z1xminb	47	18	.	.	.
cord-299281-5z1xminb	48	1	The	the	DT
cord-299281-5z1xminb	48	2	second	second	JJ
cord-299281-5z1xminb	48	3	mechanism	mechanism	NN
cord-299281-5z1xminb	48	4	invokes	invoke	VBZ
cord-299281-5z1xminb	48	5	changes	change	NNS
cord-299281-5z1xminb	48	6	in	in	IN
cord-299281-5z1xminb	48	7	the	the	DT
cord-299281-5z1xminb	48	8	tertiary	tertiary	JJ
cord-299281-5z1xminb	48	9	or	or	CC
cord-299281-5z1xminb	48	10	quaternary	quaternary	JJ
cord-299281-5z1xminb	48	11	structure	structure	NN
cord-299281-5z1xminb	48	12	of	of	IN
cord-299281-5z1xminb	48	13	the	the	DT
cord-299281-5z1xminb	48	14	protein	protein	NN
cord-299281-5z1xminb	48	15	that	that	WDT
cord-299281-5z1xminb	48	16	are	be	VBP
cord-299281-5z1xminb	48	17	induced	induce	VBN
cord-299281-5z1xminb	48	18	via	via	IN
cord-299281-5z1xminb	48	19	the	the	DT
cord-299281-5z1xminb	48	20	membrane	membrane	NN
cord-299281-5z1xminb	48	21	-	-	HYPH
cord-299281-5z1xminb	48	22	spanning	span	VBG
cord-299281-5z1xminb	48	23	domain	domain	NN
cord-299281-5z1xminb	48	24	when	when	WRB
cord-299281-5z1xminb	48	25	the	the	DT
cord-299281-5z1xminb	48	26	protein	protein	NN
cord-299281-5z1xminb	48	27	encounters	encounter	VBZ
cord-299281-5z1xminb	48	28	a	a	DT
cord-299281-5z1xminb	48	29	particular	particular	JJ
cord-299281-5z1xminb	48	30	microenvironment	microenvironment	NN
cord-299281-5z1xminb	48	31	.	.	.
cord-299281-5z1xminb	49	1	Changes	change	NNS
cord-299281-5z1xminb	49	2	in	in	IN
cord-299281-5z1xminb	49	3	protein	protein	NN
cord-299281-5z1xminb	49	4	structure	structure	NN
cord-299281-5z1xminb	49	5	could	could	MD
cord-299281-5z1xminb	49	6	include	include	VB
cord-299281-5z1xminb	49	7	oligomerization	oligomerization	NN
cord-299281-5z1xminb	49	8	or	or	CC
cord-299281-5z1xminb	49	9	aggregation	aggregation	NN
cord-299281-5z1xminb	49	10	(	(	-LRB-
cord-299281-5z1xminb	49	11	covalent	covalent	JJ
cord-299281-5z1xminb	49	12	or	or	CC
cord-299281-5z1xminb	49	13	non	non	JJ
cord-299281-5z1xminb	49	14	-	-	JJ
cord-299281-5z1xminb	49	15	covalent	covalent	JJ
cord-299281-5z1xminb	49	16	)	)	-RRB-
cord-299281-5z1xminb	49	17	with	with	IN
cord-299281-5z1xminb	49	18	other	other	JJ
cord-299281-5z1xminb	49	19	proteins	protein	NNS
cord-299281-5z1xminb	49	20	or	or	CC
cord-299281-5z1xminb	49	21	lipids	lipid	NNS
cord-299281-5z1xminb	49	22	,	,	,
cord-299281-5z1xminb	49	23	which	which	WDT
cord-299281-5z1xminb	49	24	would	would	MD
cord-299281-5z1xminb	49	25	prevent	prevent	VB
cord-299281-5z1xminb	49	26	movement	movement	NN
cord-299281-5z1xminb	49	27	of	of	IN
cord-299281-5z1xminb	49	28	the	the	DT
cord-299281-5z1xminb	49	29	protein	protein	NN
cord-299281-5z1xminb	49	30	into	into	IN
cord-299281-5z1xminb	49	31	transport	transport	NN
cord-299281-5z1xminb	49	32	vesicles	vesicle	NNS
cord-299281-5z1xminb	49	33	.	.	.
cord-299281-5z1xminb	50	1	The	the	DT
cord-299281-5z1xminb	50	2	cis	cis	NNP
cord-299281-5z1xminb	50	3	-	-	HYPH
cord-299281-5z1xminb	50	4	Golgi	Golgi	NNP
cord-299281-5z1xminb	50	5	may	may	MD
cord-299281-5z1xminb	50	6	differ	differ	VB
cord-299281-5z1xminb	50	7	from	from	IN
cord-299281-5z1xminb	50	8	the	the	DT
cord-299281-5z1xminb	50	9	ER	ER	NNP
cord-299281-5z1xminb	50	10	in	in	IN
cord-299281-5z1xminb	50	11	lipid	lipid	NN
cord-299281-5z1xminb	50	12	composition	composition	NN
cord-299281-5z1xminb	50	13	and	and	CC
cord-299281-5z1xminb	50	14	divalent	divalent	JJ
cord-299281-5z1xminb	50	15	cation	cation	NN
cord-299281-5z1xminb	50	16	concentration	concentration	NN
cord-299281-5z1xminb	50	17	.	.	.
cord-299281-5z1xminb	51	1	These	these	DT
cord-299281-5z1xminb	51	2	changes	change	NNS
cord-299281-5z1xminb	51	3	in	in	IN
cord-299281-5z1xminb	51	4	microenvironment	microenvironment	NN
cord-299281-5z1xminb	51	5	could	could	MD
cord-299281-5z1xminb	51	6	conceivably	conceivably	RB
cord-299281-5z1xminb	51	7	trigger	trigger	VB
cord-299281-5z1xminb	51	8	a	a	DT
cord-299281-5z1xminb	51	9	conformational	conformational	JJ
cord-299281-5z1xminb	51	10	shift	shift	NN
cord-299281-5z1xminb	51	11	in	in	IN
cord-299281-5z1xminb	51	12	Golgi	Golgi	NNP
cord-299281-5z1xminb	51	13	resident	resident	NN
cord-299281-5z1xminb	51	14	proteins	protein	NNS
cord-299281-5z1xminb	51	15	upon	upon	IN
cord-299281-5z1xminb	51	16	their	-PRON-	PRP$
cord-299281-5z1xminb	51	17	arrival	arrival	NN
cord-299281-5z1xminb	51	18	in	in	IN
cord-299281-5z1xminb	51	19	the	the	DT
cord-299281-5z1xminb	51	20	appropriate	appropriate	JJ
cord-299281-5z1xminb	51	21	compartment	compartment	NN
cord-299281-5z1xminb	51	22	.	.	.
cord-299281-5z1xminb	52	1	We	-PRON-	PRP
cord-299281-5z1xminb	52	2	have	have	VBP
cord-299281-5z1xminb	52	3	been	be	VBN
cord-299281-5z1xminb	52	4	investigating	investigate	VBG
cord-299281-5z1xminb	52	5	the	the	DT
cord-299281-5z1xminb	52	6	mechanisms	mechanism	NNS
cord-299281-5z1xminb	52	7	by	by	IN
cord-299281-5z1xminb	52	8	which	which	WDT
cord-299281-5z1xminb	52	9	ml	ml	NNS
cord-299281-5z1xminb	52	10	might	may	MD
cord-299281-5z1xminb	52	11	mediate	mediate	VB
cord-299281-5z1xminb	52	12	retention	retention	NN
cord-299281-5z1xminb	52	13	of	of	IN
cord-299281-5z1xminb	52	14	Gml	Gml	NNP
cord-299281-5z1xminb	52	15	in	in	IN
cord-299281-5z1xminb	52	16	the	the	DT
cord-299281-5z1xminb	52	17	Golgi	Golgi	NNP
cord-299281-5z1xminb	52	18	complex	complex	NN
cord-299281-5z1xminb	52	19	.	.	.
cord-299281-5z1xminb	53	1	We	-PRON-	PRP
cord-299281-5z1xminb	53	2	observed	observe	VBD
cord-299281-5z1xminb	53	3	that	that	IN
cord-299281-5z1xminb	53	4	Gml	Gml	NNP
cord-299281-5z1xminb	53	5	formed	form	VBD
cord-299281-5z1xminb	53	6	a	a	DT
cord-299281-5z1xminb	53	7	large	large	JJ
cord-299281-5z1xminb	53	8	oligomer	oligomer	NN
cord-299281-5z1xminb	53	9	soon	soon	RB
cord-299281-5z1xminb	53	10	after	after	IN
cord-299281-5z1xminb	53	11	synthesis	synthesis	NN
cord-299281-5z1xminb	53	12	when	when	WRB
cord-299281-5z1xminb	53	13	assayed	assay	VBN
cord-299281-5z1xminb	53	14	by	by	IN
cord-299281-5z1xminb	53	15	sucrose	sucrose	NN
cord-299281-5z1xminb	53	16	gradient	gradient	NN
cord-299281-5z1xminb	53	17	centrifugation	centrifugation	NN
cord-299281-5z1xminb	53	18	.	.	.
cord-299281-5z1xminb	54	1	Here	here	RB
cord-299281-5z1xminb	54	2	,	,	,
cord-299281-5z1xminb	54	3	we	-PRON-	PRP
cord-299281-5z1xminb	54	4	show	show	VBP
cord-299281-5z1xminb	54	5	that	that	IN
cord-299281-5z1xminb	54	6	this	this	DT
cord-299281-5z1xminb	54	7	oligomer	oligomer	NN
cord-299281-5z1xminb	54	8	is	be	VBZ
cord-299281-5z1xminb	54	9	unusually	unusually	RB
cord-299281-5z1xminb	54	10	stable	stable	JJ
cord-299281-5z1xminb	54	11	,	,	,
cord-299281-5z1xminb	54	12	and	and	CC
cord-299281-5z1xminb	54	13	that	that	IN
cord-299281-5z1xminb	54	14	its	-PRON-	PRP$
cord-299281-5z1xminb	54	15	formation	formation	NN
cord-299281-5z1xminb	54	16	correlates	correlate	VBZ
cord-299281-5z1xminb	54	17	with	with	IN
cord-299281-5z1xminb	54	18	arrival	arrival	NN
cord-299281-5z1xminb	54	19	and	and	CC
cord-299281-5z1xminb	54	20	retention	retention	NN
cord-299281-5z1xminb	54	21	in	in	IN
cord-299281-5z1xminb	54	22	the	the	DT
cord-299281-5z1xminb	54	23	Golgi	Golgi	NNP
cord-299281-5z1xminb	54	24	complex	complex	NN
cord-299281-5z1xminb	54	25	.	.	.
cord-299281-5z1xminb	55	1	Our	-PRON-	PRP$
cord-299281-5z1xminb	55	2	findings	finding	NNS
cord-299281-5z1xminb	55	3	point	point	VBP
cord-299281-5z1xminb	55	4	to	to	IN
cord-299281-5z1xminb	55	5	a	a	DT
cord-299281-5z1xminb	55	6	possible	possible	JJ
cord-299281-5z1xminb	55	7	role	role	NN
cord-299281-5z1xminb	55	8	for	for	IN
cord-299281-5z1xminb	55	9	oligomerization	oligomerization	NN
cord-299281-5z1xminb	55	10	in	in	IN
cord-299281-5z1xminb	55	11	the	the	DT
cord-299281-5z1xminb	55	12	retention	retention	NN
cord-299281-5z1xminb	55	13	of	of	IN
cord-299281-5z1xminb	55	14	Gml	Gml	NNP
cord-299281-5z1xminb	55	15	.	.	.
cord-299281-5z1xminb	56	1	Aprotinin	Aprotinin	NNP
cord-299281-5z1xminb	56	2	,	,	,
cord-299281-5z1xminb	56	3	colchicine	colchicine	NN
cord-299281-5z1xminb	56	4	,	,	,
cord-299281-5z1xminb	56	5	carbonyl	carbonyl	NN
cord-299281-5z1xminb	56	6	cyanide	cyanide	NN
cord-299281-5z1xminb	56	7	m	m	NNP
cord-299281-5z1xminb	56	8	-	-	HYPH
cord-299281-5z1xminb	56	9	chlorophenylhydrazone	chlorophenylhydrazone	NNP
cord-299281-5z1xminb	56	10	(	(	-LRB-
cord-299281-5z1xminb	56	11	CCCP	CCCP	NNP
cord-299281-5z1xminb	56	12	)	)	-RRB-
cord-299281-5z1xminb	56	13	,	,	,
cord-299281-5z1xminb	56	14	cytochalasin	cytochalasin	FW
cord-299281-5z1xminb	56	15	D	D	NNP
cord-299281-5z1xminb	56	16	,	,	,
cord-299281-5z1xminb	56	17	TPCK	TPCK	NNP
cord-299281-5z1xminb	56	18	-	-	HYPH
cord-299281-5z1xminb	56	19	trypsin	trypsin	NN
cord-299281-5z1xminb	56	20	,	,	,
cord-299281-5z1xminb	56	21	and	and	CC
cord-299281-5z1xminb	56	22	soybean	soybean	NN
cord-299281-5z1xminb	56	23	trypsin	trypsin	NN
cord-299281-5z1xminb	56	24	inhibitor	inhibitor	NN
cord-299281-5z1xminb	56	25	were	be	VBD
cord-299281-5z1xminb	56	26	obtained	obtain	VBN
cord-299281-5z1xminb	56	27	from	from	IN
cord-299281-5z1xminb	56	28	Sigma	Sigma	NNP
cord-299281-5z1xminb	56	29	Chem	Chem	NNP
cord-299281-5z1xminb	56	30	.	.	.
cord-299281-5z1xminb	57	1	Co.	Co.	NNP
cord-299281-5z1xminb	58	1	(	(	-LRB-
cord-299281-5z1xminb	58	2	St.	St.	NNP
cord-299281-5z1xminb	58	3	Louis	Louis	NNP
cord-299281-5z1xminb	58	4	,	,	,
cord-299281-5z1xminb	58	5	MO	MO	NNP
cord-299281-5z1xminb	58	6	)	)	-RRB-
cord-299281-5z1xminb	58	7	.	.	.
cord-299281-5z1xminb	59	1	Brefeldin	Brefeldin	NNP
cord-299281-5z1xminb	59	2	A	A	NNP
cord-299281-5z1xminb	59	3	was	be	VBD
cord-299281-5z1xminb	59	4	from	from	IN
cord-299281-5z1xminb	59	5	Epieentre	Epieentre	NNP
cord-299281-5z1xminb	59	6	Technologies	Technologies	NNP
cord-299281-5z1xminb	59	7	(	(	-LRB-
cord-299281-5z1xminb	59	8	Madison	Madison	NNP
cord-299281-5z1xminb	59	9	,	,	,
cord-299281-5z1xminb	59	10	WI	WI	NNP
cord-299281-5z1xminb	59	11	)	)	-RRB-
cord-299281-5z1xminb	59	12	.	.	.
cord-299281-5z1xminb	60	1	Stock	stock	NN
cord-299281-5z1xminb	60	2	solutions	solution	NNS
cord-299281-5z1xminb	60	3	of	of	IN
cord-299281-5z1xminb	60	4	reagents	reagent	NNS
cord-299281-5z1xminb	60	5	were	be	VBD
cord-299281-5z1xminb	60	6	stored	store	VBN
cord-299281-5z1xminb	60	7	at	at	IN
cord-299281-5z1xminb	60	8	-20	-20	NNP
cord-299281-5z1xminb	60	9	°	°	NN
cord-299281-5z1xminb	60	10	C	C	NNP
cord-299281-5z1xminb	60	11	.	.	.
cord-299281-5z1xminb	61	1	COS-'/and	cos-'/and	CC
cord-299281-5z1xminb	61	2	HeLa	HeLa	NNP
cord-299281-5z1xminb	61	3	cells	cell	NNS
cord-299281-5z1xminb	61	4	were	be	VBD
cord-299281-5z1xminb	61	5	maintained	maintain	VBN
cord-299281-5z1xminb	61	6	in	in	IN
cord-299281-5z1xminb	61	7	DME	DME	NNP
cord-299281-5z1xminb	61	8	with	with	IN
cord-299281-5z1xminb	61	9	10	10	CD
cord-299281-5z1xminb	61	10	and	and	CC
cord-299281-5z1xminb	61	11	5	5	CD
cord-299281-5z1xminb	61	12	%	%	NN
cord-299281-5z1xminb	61	13	FCS	FCS	NNP
cord-299281-5z1xminb	61	14	,	,	,
cord-299281-5z1xminb	61	15	respectively	respectively	RB
cord-299281-5z1xminb	61	16	.	.	.
cord-299281-5z1xminb	62	1	COS-7	cos-7	NN
cord-299281-5z1xminb	62	2	cells	cell	NNS
cord-299281-5z1xminb	62	3	plated	plate	VBN
cord-299281-5z1xminb	62	4	in	in	IN
cord-299281-5z1xminb	62	5	35-mm	35-mm	CD
cord-299281-5z1xminb	62	6	dishes	dish	NNS
cord-299281-5z1xminb	62	7	(	(	-LRB-
cord-299281-5z1xminb	62	8	40	40	CD
cord-299281-5z1xminb	62	9	-	-	SYM
cord-299281-5z1xminb	62	10	70	70	CD
cord-299281-5z1xminb	62	11	%	%	NN
cord-299281-5z1xminb	62	12	confluent	confluent	JJ
cord-299281-5z1xminb	62	13	)	)	-RRB-
cord-299281-5z1xminb	62	14	were	be	VBD
cord-299281-5z1xminb	62	15	transfected	transfecte	VBN
cord-299281-5z1xminb	62	16	with	with	IN
cord-299281-5z1xminb	62	17	an	an	DT
cord-299281-5z1xminb	62	18	SV40-based	sv40-based	JJ
cord-299281-5z1xminb	62	19	expression	expression	NN
cord-299281-5z1xminb	62	20	vector	vector	NN
cord-299281-5z1xminb	62	21	using	use	VBG
cord-299281-5z1xminb	62	22	DEAE	DEAE	NNP
cord-299281-5z1xminb	62	23	-	-	HYPH
cord-299281-5z1xminb	62	24	dextran	dextran	NN
cord-299281-5z1xminb	62	25	(	(	-LRB-
cord-299281-5z1xminb	62	26	Machamer	Machamer	NNP
cord-299281-5z1xminb	62	27	et	et	NNP
cord-299281-5z1xminb	62	28	al	al	NNP
cord-299281-5z1xminb	62	29	.	.	NNP
cord-299281-5z1xminb	62	30	,	,	,
cord-299281-5z1xminb	62	31	1985	1985	CD
cord-299281-5z1xminb	62	32	)	)	-RRB-
cord-299281-5z1xminb	62	33	.	.	.
cord-299281-5z1xminb	63	1	Expression	expression	NN
cord-299281-5z1xminb	63	2	was	be	VBD
cord-299281-5z1xminb	63	3	analyzed	analyze	VBN
cord-299281-5z1xminb	63	4	at	at	IN
cord-299281-5z1xminb	63	5	40	40	CD
cord-299281-5z1xminb	63	6	-	-	HYPH
cord-299281-5z1xminb	63	7	-48	-48	NN
cord-299281-5z1xminb	63	8	h	h	NN
cord-299281-5z1xminb	63	9	posttransfection	posttransfection	NN
cord-299281-5z1xminb	63	10	.	.	.
cord-299281-5z1xminb	64	1	For	for	IN
cord-299281-5z1xminb	64	2	expression	expression	NN
cord-299281-5z1xminb	64	3	using	use	VBG
cord-299281-5z1xminb	64	4	the	the	DT
cord-299281-5z1xminb	64	5	vaccinia	vaccinia	NN
cord-299281-5z1xminb	64	6	-	-	HYPH
cord-299281-5z1xminb	64	7	T7	t7	NN
cord-299281-5z1xminb	64	8	system	system	NN
cord-299281-5z1xminb	64	9	,	,	,
cord-299281-5z1xminb	64	10	HeLa	HeLa	NNP
cord-299281-5z1xminb	64	11	cells	cell	NNS
cord-299281-5z1xminb	64	12	(	(	-LRB-
cord-299281-5z1xminb	64	13	40	40	CD
cord-299281-5z1xminb	64	14	-	-	SYM
cord-299281-5z1xminb	64	15	70	70	CD
cord-299281-5z1xminb	64	16	%	%	NN
cord-299281-5z1xminb	64	17	confluent	confluent	JJ
cord-299281-5z1xminb	64	18	)	)	-RRB-
cord-299281-5z1xminb	64	19	were	be	VBD
cord-299281-5z1xminb	64	20	infected	infect	VBN
cord-299281-5z1xminb	64	21	with	with	IN
cord-299281-5z1xminb	64	22	the	the	DT
cord-299281-5z1xminb	64	23	recombinant	recombinant	JJ
cord-299281-5z1xminb	64	24	vaccinia	vaccinia	NN
cord-299281-5z1xminb	64	25	virus	virus	NN
cord-299281-5z1xminb	64	26	vTF7	vTF7	NNP
cord-299281-5z1xminb	64	27	-	-	HYPH
cord-299281-5z1xminb	64	28	3	3	CD
cord-299281-5z1xminb	64	29	encoding	encoding	NN
cord-299281-5z1xminb	64	30	T7	T7	NNP
cord-299281-5z1xminb	64	31	RNA	RNA	NNP
cord-299281-5z1xminb	64	32	polymerase	polymerase	NN
cord-299281-5z1xminb	65	1	(	(	-LRB-
cord-299281-5z1xminb	65	2	Fuerst	Fuerst	NNP
cord-299281-5z1xminb	65	3	et	et	NNP
cord-299281-5z1xminb	65	4	al	al	NNP
cord-299281-5z1xminb	65	5	.	.	.
cord-299281-5z1xminb	66	1	,	,	,
cord-299281-5z1xminb	66	2	1986	1986	CD
cord-299281-5z1xminb	66	3	)	)	-RRB-
cord-299281-5z1xminb	66	4	at	at	IN
cord-299281-5z1xminb	66	5	a	a	DT
cord-299281-5z1xminb	66	6	multiplicity	multiplicity	NN
cord-299281-5z1xminb	66	7	of	of	IN
cord-299281-5z1xminb	66	8	infection	infection	NN
cord-299281-5z1xminb	66	9	of	of	IN
cord-299281-5z1xminb	66	10	10	10	CD
cord-299281-5z1xminb	66	11	-	-	SYM
cord-299281-5z1xminb	66	12	20	20	CD
cord-299281-5z1xminb	66	13	.	.	.
cord-299281-5z1xminb	67	1	After	after	IN
cord-299281-5z1xminb	67	2	adsorption	adsorption	NN
cord-299281-5z1xminb	67	3	for	for	IN
cord-299281-5z1xminb	67	4	30	30	CD
cord-299281-5z1xminb	67	5	min	min	NN
cord-299281-5z1xminb	67	6	at	at	IN
cord-299281-5z1xminb	67	7	37	37	CD
cord-299281-5z1xminb	67	8	°	°	NN
cord-299281-5z1xminb	67	9	C	c	NN
cord-299281-5z1xminb	67	10	,	,	,
cord-299281-5z1xminb	67	11	the	the	DT
cord-299281-5z1xminb	67	12	inoculum	inoculum	NN
cord-299281-5z1xminb	67	13	was	be	VBD
cord-299281-5z1xminb	67	14	replaced	replace	VBN
cord-299281-5z1xminb	67	15	with	with	IN
cord-299281-5z1xminb	67	16	0.75	0.75	CD
cord-299281-5z1xminb	67	17	ml	ml	NNS
cord-299281-5z1xminb	67	18	of	of	IN
cord-299281-5z1xminb	67	19	serum	serum	NN
cord-299281-5z1xminb	67	20	-	-	HYPH
cord-299281-5z1xminb	67	21	free	free	JJ
cord-299281-5z1xminb	67	22	medium	medium	NN
cord-299281-5z1xminb	67	23	containing	contain	VBG
cord-299281-5z1xminb	67	24	2/~g	2/~g	CD
cord-299281-5z1xminb	67	25	of	of	IN
cord-299281-5z1xminb	67	26	a	a	DT
cord-299281-5z1xminb	67	27	vector	vector	NN
cord-299281-5z1xminb	67	28	(	(	-LRB-
cord-299281-5z1xminb	67	29	pAR2529	pAR2529	NNP
cord-299281-5z1xminb	67	30	)	)	-RRB-
cord-299281-5z1xminb	67	31	encoding	encode	VBG
cord-299281-5z1xminb	67	32	the	the	DT
cord-299281-5z1xminb	67	33	appropriate	appropriate	JJ
cord-299281-5z1xminb	67	34	gene	gene	NN
cord-299281-5z1xminb	67	35	behind	behind	IN
cord-299281-5z1xminb	67	36	the	the	DT
cord-299281-5z1xminb	67	37	T7	T7	NNP
cord-299281-5z1xminb	67	38	promoter	promoter	NN
cord-299281-5z1xminb	67	39	and	and	CC
cord-299281-5z1xminb	67	40	5	5	CD
cord-299281-5z1xminb	67	41	-	-	SYM
cord-299281-5z1xminb	67	42	10/~1	10/~1	CD
cord-299281-5z1xminb	67	43	of	of	IN
cord-299281-5z1xminb	67	44	the	the	DT
cord-299281-5z1xminb	67	45	cationic	cationic	JJ
cord-299281-5z1xminb	67	46	lipid	lipid	NN
cord-299281-5z1xminb	68	1	~TransfectACE	~TransfectACE	NFP
cord-299281-5z1xminb	68	2	"	"	''
cord-299281-5z1xminb	68	3	(	(	-LRB-
cord-299281-5z1xminb	68	4	GIBCO	GIBCO	NNP
cord-299281-5z1xminb	68	5	BRL	BRL	NNP
cord-299281-5z1xminb	68	6	,	,	,
cord-299281-5z1xminb	68	7	Gaithersburg	Gaithersburg	NNP
cord-299281-5z1xminb	68	8	,	,	,
cord-299281-5z1xminb	68	9	MD	MD	NNP
cord-299281-5z1xminb	68	10	;	;	:
cord-299281-5z1xminb	68	11	Rose	Rose	NNP
cord-299281-5z1xminb	68	12	et	et	FW
cord-299281-5z1xminb	68	13	al	al	NNP
cord-299281-5z1xminb	68	14	.	.	NNP
cord-299281-5z1xminb	68	15	,	,	,
cord-299281-5z1xminb	68	16	1991	1991	CD
cord-299281-5z1xminb	68	17	)	)	-RRB-
cord-299281-5z1xminb	68	18	.	.	.
cord-299281-5z1xminb	69	1	Expression	expression	NN
cord-299281-5z1xminb	69	2	level	level	NN
cord-299281-5z1xminb	69	3	was	be	VBD
cord-299281-5z1xminb	69	4	varied	vary	VBN
cord-299281-5z1xminb	69	5	by	by	IN
cord-299281-5z1xminb	69	6	changing	change	VBG
cord-299281-5z1xminb	69	7	the	the	DT
cord-299281-5z1xminb	69	8	amount	amount	NN
cord-299281-5z1xminb	69	9	of	of	IN
cord-299281-5z1xminb	69	10	DNA	dna	NN
cord-299281-5z1xminb	69	11	added	add	VBN
cord-299281-5z1xminb	69	12	per	per	IN
cord-299281-5z1xminb	69	13	well	well	RB
cord-299281-5z1xminb	69	14	(	(	-LRB-
cord-299281-5z1xminb	69	15	0.02	0.02	CD
cord-299281-5z1xminb	69	16	-	-	SYM
cord-299281-5z1xminb	69	17	5	5	CD
cord-299281-5z1xminb	69	18	~tg	~tg	NNS
cord-299281-5z1xminb	69	19	/	/	SYM
cord-299281-5z1xminb	69	20	dish	dish	NN
cord-299281-5z1xminb	69	21	)	)	-RRB-
cord-299281-5z1xminb	69	22	.	.	.
cord-299281-5z1xminb	70	1	In	in	IN
cord-299281-5z1xminb	70	2	coexpression	coexpression	NN
cord-299281-5z1xminb	70	3	experiments	experiment	NNS
cord-299281-5z1xminb	70	4	,	,	,
cord-299281-5z1xminb	70	5	cells	cell	NNS
cord-299281-5z1xminb	70	6	were	be	VBD
cord-299281-5z1xminb	70	7	transfected	transfecte	VBN
cord-299281-5z1xminb	70	8	with	with	IN
cord-299281-5z1xminb	70	9	1/~g	1/~g	CD
cord-299281-5z1xminb	70	10	of	of	IN
cord-299281-5z1xminb	70	11	DNA	dna	NN
cord-299281-5z1xminb	70	12	encoding	encoding	NN
cord-299281-5z1xminb	70	13	each	each	DT
cord-299281-5z1xminb	70	14	construct	construct	NN
cord-299281-5z1xminb	70	15	.	.	.
cord-299281-5z1xminb	71	1	Expression	expression	NN
cord-299281-5z1xminb	71	2	was	be	VBD
cord-299281-5z1xminb	71	3	analyzed	analyze	VBN
cord-299281-5z1xminb	71	4	by	by	IN
cord-299281-5z1xminb	71	5	metabolic	metabolic	JJ
cord-299281-5z1xminb	71	6	labeling	labeling	NN
cord-299281-5z1xminb	71	7	starting	start	VBG
cord-299281-5z1xminb	71	8	3	3	CD
cord-299281-5z1xminb	71	9	h	h	NN
cord-299281-5z1xminb	71	10	after	after	IN
cord-299281-5z1xminb	71	11	infection	infection	NN
cord-299281-5z1xminb	71	12	.	.	.
cord-299281-5z1xminb	72	1	Gml	Gml	NNP
cord-299281-5z1xminb	72	2	and	and	CC
cord-299281-5z1xminb	72	3	related	related	JJ
cord-299281-5z1xminb	72	4	mutants	mutant	NNS
cord-299281-5z1xminb	72	5	were	be	VBD
cord-299281-5z1xminb	72	6	generated	generate	VBN
cord-299281-5z1xminb	72	7	using	use	VBG
cord-299281-5z1xminb	72	8	the	the	DT
cord-299281-5z1xminb	72	9	Kunkel	Kunkel	NNP
cord-299281-5z1xminb	72	10	method	method	NN
cord-299281-5z1xminb	72	11	of	of	IN
cord-299281-5z1xminb	72	12	oligonucleotide	oligonucleotide	RB
cord-299281-5z1xminb	72	13	-	-	HYPH
cord-299281-5z1xminb	72	14	direeted	direete	VBN
cord-299281-5z1xminb	72	15	mutagenesis	mutagenesis	NN
cord-299281-5z1xminb	72	16	as	as	IN
cord-299281-5z1xminb	72	17	described	describe	VBN
cord-299281-5z1xminb	72	18	previously	previously	RB
cord-299281-5z1xminb	72	19	(	(	-LRB-
cord-299281-5z1xminb	72	20	Swift	Swift	NNP
cord-299281-5z1xminb	72	21	and	and	CC
cord-299281-5z1xminb	72	22	Machamer	Machamer	NNP
cord-299281-5z1xminb	72	23	,	,	,
cord-299281-5z1xminb	72	24	1991	1991	CD
cord-299281-5z1xminb	72	25	;	;	:
cord-299281-5z1xminb	73	1	Machamer	Machamer	NNP
cord-299281-5z1xminb	73	2	et	et	NNP
cord-299281-5z1xminb	73	3	al	al	NNP
cord-299281-5z1xminb	73	4	.	.	NNP
cord-299281-5z1xminb	73	5	,	,	,
cord-299281-5z1xminb	73	6	1993	1993	CD
cord-299281-5z1xminb	73	7	)	)	-RRB-
cord-299281-5z1xminb	73	8	.	.	.
cord-299281-5z1xminb	74	1	Point	point	NN
cord-299281-5z1xminb	74	2	mutants	mutant	NNS
cord-299281-5z1xminb	74	3	were	be	VBD
cord-299281-5z1xminb	74	4	named	name	VBN
cord-299281-5z1xminb	74	5	by	by	IN
cord-299281-5z1xminb	74	6	appending	append	VBG
cord-299281-5z1xminb	74	7	the	the	DT
cord-299281-5z1xminb	74	8	original	original	JJ
cord-299281-5z1xminb	74	9	amino	amino	NN
cord-299281-5z1xminb	74	10	acid	acid	NN
cord-299281-5z1xminb	74	11	(	(	-LRB-
cord-299281-5z1xminb	74	12	in	in	IN
cord-299281-5z1xminb	74	13	single	single	JJ
cord-299281-5z1xminb	74	14	letter	letter	NN
cord-299281-5z1xminb	74	15	code	code	NN
cord-299281-5z1xminb	74	16	)	)	-RRB-
cord-299281-5z1xminb	74	17	followed	follow	VBN
cord-299281-5z1xminb	74	18	by	by	IN
cord-299281-5z1xminb	74	19	the	the	DT
cord-299281-5z1xminb	74	20	new	new	JJ
cord-299281-5z1xminb	74	21	amino	amino	NN
cord-299281-5z1xminb	74	22	acid	acid	NN
cord-299281-5z1xminb	74	23	.	.	.
cord-299281-5z1xminb	75	1	,	,	,
cord-299281-5z1xminb	75	2	m	m	NNP
cord-299281-5z1xminb	75	3	(	(	-LRB-
cord-299281-5z1xminb	75	4	Guan	Guan	NNP
cord-299281-5z1xminb	75	5	et	et	FW
cord-299281-5z1xminb	75	6	al	al	NNP
cord-299281-5z1xminb	75	7	.	.	NNP
cord-299281-5z1xminb	75	8	,	,	,
cord-299281-5z1xminb	75	9	1988a	1988a	CD
cord-299281-5z1xminb	75	10	)	)	-RRB-
cord-299281-5z1xminb	75	11	and	and	CC
cord-299281-5z1xminb	75	12	TMR	TMR	NNP
cord-299281-5z1xminb	75	13	(	(	-LRB-
cord-299281-5z1xminb	75	14	Doms	Doms	NNPS
cord-299281-5z1xminb	75	15	et	et	FW
cord-299281-5z1xminb	75	16	al	al	NNP
cord-299281-5z1xminb	75	17	.	.	NNP
cord-299281-5z1xminb	75	18	,	,	,
cord-299281-5z1xminb	75	19	1988	1988	CD
cord-299281-5z1xminb	75	20	)	)	-RRB-
cord-299281-5z1xminb	75	21	were	be	VBD
cord-299281-5z1xminb	75	22	obtained	obtain	VBN
cord-299281-5z1xminb	75	23	from	from	IN
cord-299281-5z1xminb	75	24	Jack	Jack	NNP
cord-299281-5z1xminb	75	25	Rose	Rose	NNP
cord-299281-5z1xminb	75	26	(	(	-LRB-
cord-299281-5z1xminb	75	27	Yale	Yale	NNP
cord-299281-5z1xminb	75	28	University	University	NNP
cord-299281-5z1xminb	75	29	)	)	-RRB-
cord-299281-5z1xminb	75	30	.	.	.
cord-299281-5z1xminb	76	1	Construction	construction	NN
cord-299281-5z1xminb	76	2	of	of	IN
cord-299281-5z1xminb	76	3	,	,	,
cord-299281-5z1xminb	76	4	~mlG	~mlG	NNP
cord-299281-5z1xminb	76	5	was	be	VBD
cord-299281-5z1xminb	76	6	described	describe	VBN
cord-299281-5z1xminb	76	7	previously	previously	RB
cord-299281-5z1xminb	76	8	(	(	-LRB-
cord-299281-5z1xminb	76	9	Swift	Swift	NNP
cord-299281-5z1xminb	76	10	and	and	CC
cord-299281-5z1xminb	76	11	Machamer	Machamer	NNP
cord-299281-5z1xminb	76	12	,	,	,
cord-299281-5z1xminb	76	13	1991	1991	CD
cord-299281-5z1xminb	76	14	)	)	-RRB-
cord-299281-5z1xminb	76	15	.	.	.
cord-299281-5z1xminb	77	1	tGml	tGml	NNP
cord-299281-5z1xminb	77	2	was	be	VBD
cord-299281-5z1xminb	77	3	constructed	construct	VBN
cord-299281-5z1xminb	77	4	from	from	IN
cord-299281-5z1xminb	77	5	Gml	Gml	NNP
cord-299281-5z1xminb	77	6	by	by	IN
cord-299281-5z1xminb	77	7	inserting	insert	VBG
cord-299281-5z1xminb	77	8	a	a	DT
cord-299281-5z1xminb	77	9	stop	stop	NN
cord-299281-5z1xminb	77	10	codon	codon	NN
cord-299281-5z1xminb	77	11	at	at	IN
cord-299281-5z1xminb	77	12	position	position	NN
cord-299281-5z1xminb	77	13	487	487	CD
cord-299281-5z1xminb	77	14	using	use	VBG
cord-299281-5z1xminb	77	15	site	site	NN
cord-299281-5z1xminb	77	16	-	-	HYPH
cord-299281-5z1xminb	77	17	directed	direct	VBN
cord-299281-5z1xminb	77	18	mutagenesis	mutagenesis	NN
cord-299281-5z1xminb	77	19	(	(	-LRB-
cord-299281-5z1xminb	77	20	Kunkel	Kunkel	NNP
cord-299281-5z1xminb	77	21	et	et	NNP
cord-299281-5z1xminb	77	22	al	al	NNP
cord-299281-5z1xminb	77	23	.	.	NNP
cord-299281-5z1xminb	77	24	,	,	,
cord-299281-5z1xminb	77	25	1987	1987	CD
cord-299281-5z1xminb	77	26	)	)	-RRB-
cord-299281-5z1xminb	77	27	.	.	.
cord-299281-5z1xminb	78	1	Indirect	indirect	JJ
cord-299281-5z1xminb	78	2	immunofluorescence	immunofluorescence	NN
cord-299281-5z1xminb	78	3	staining	staining	NN
cord-299281-5z1xminb	78	4	of	of	IN
cord-299281-5z1xminb	78	5	transiently	transiently	RB
cord-299281-5z1xminb	78	6	transfected	transfecte	VBN
cord-299281-5z1xminb	78	7	COS-7	COS-7	NNP
cord-299281-5z1xminb	78	8	ceils	ceil	NNS
cord-299281-5z1xminb	78	9	was	be	VBD
cord-299281-5z1xminb	78	10	performed	perform	VBN
cord-299281-5z1xminb	78	11	as	as	IN
cord-299281-5z1xminb	78	12	described	describe	VBN
cord-299281-5z1xminb	78	13	(	(	-LRB-
cord-299281-5z1xminb	78	14	Machamer	Machamer	NNP
cord-299281-5z1xminb	78	15	and	and	CC
cord-299281-5z1xminb	78	16	Rose	Rose	NNP
cord-299281-5z1xminb	78	17	,	,	,
cord-299281-5z1xminb	78	18	1987	1987	CD
cord-299281-5z1xminb	78	19	;	;	:
cord-299281-5z1xminb	78	20	Machamer	Machamer	NNP
cord-299281-5z1xminb	78	21	and	and	CC
cord-299281-5z1xminb	78	22	Rose	Rose	NNP
cord-299281-5z1xminb	78	23	,	,	,
cord-299281-5z1xminb	78	24	1988	1988	CD
cord-299281-5z1xminb	78	25	)	)	-RRB-
cord-299281-5z1xminb	78	26	.	.	.
cord-299281-5z1xminb	79	1	Gml	Gml	NNP
cord-299281-5z1xminb	79	2	and	and	CC
cord-299281-5z1xminb	79	3	related	related	JJ
cord-299281-5z1xminb	79	4	mutants	mutant	NNS
cord-299281-5z1xminb	79	5	were	be	VBD
cord-299281-5z1xminb	79	6	detected	detect	VBN
cord-299281-5z1xminb	79	7	using	use	VBG
cord-299281-5z1xminb	79	8	a	a	DT
cord-299281-5z1xminb	79	9	monoclonal	monoclonal	JJ
cord-299281-5z1xminb	79	10	anti	anti	JJ
cord-299281-5z1xminb	79	11	-	-	JJ
cord-299281-5z1xminb	79	12	VSV	VSV	NNP
cord-299281-5z1xminb	79	13	G	G	NNP
cord-299281-5z1xminb	79	14	antibody	antibody	NN
cord-299281-5z1xminb	79	15	(	(	-LRB-
cord-299281-5z1xminb	79	16	5/~g	5/~g	CD
cord-299281-5z1xminb	79	17	/	/	SYM
cord-299281-5z1xminb	79	18	ml	ml	NN
cord-299281-5z1xminb	79	19	;	;	:
cord-299281-5z1xminb	79	20	Lefrancois	Lefrancois	NNP
cord-299281-5z1xminb	79	21	and	and	CC
cord-299281-5z1xminb	79	22	Lyles	Lyles	NNP
cord-299281-5z1xminb	79	23	,	,	,
cord-299281-5z1xminb	79	24	1982	1982	CD
cord-299281-5z1xminb	79	25	)	)	-RRB-
cord-299281-5z1xminb	79	26	followed	follow	VBN
cord-299281-5z1xminb	79	27	by	by	IN
cord-299281-5z1xminb	79	28	Texas	Texas	NNP
cord-299281-5z1xminb	79	29	red	red	JJ
cord-299281-5z1xminb	79	30	-	-	HYPH
cord-299281-5z1xminb	79	31	conjugated	conjugated	JJ
cord-299281-5z1xminb	79	32	,	,	,
cord-299281-5z1xminb	79	33	affinity	affinity	NN
cord-299281-5z1xminb	79	34	-	-	HYPH
cord-299281-5z1xminb	79	35	purified	purify	VBN
cord-299281-5z1xminb	79	36	goat	goat	NN
cord-299281-5z1xminb	79	37	anti	anti	JJ
cord-299281-5z1xminb	79	38	-	-	JJ
cord-299281-5z1xminb	79	39	mouse	mouse	JJ
cord-299281-5z1xminb	79	40	IgG	IgG	NNP
cord-299281-5z1xminb	79	41	(	(	-LRB-
cord-299281-5z1xminb	79	42	10	10	CD
cord-299281-5z1xminb	79	43	/zg	/zg	NNP
cord-299281-5z1xminb	79	44	/	/	SYM
cord-299281-5z1xminb	79	45	ml	ml	XX
cord-299281-5z1xminb	79	46	;	;	:
cord-299281-5z1xminb	79	47	Jackson	Jackson	NNP
cord-299281-5z1xminb	79	48	Immuno	Immuno	NNP
cord-299281-5z1xminb	79	49	Research	Research	NNP
cord-299281-5z1xminb	79	50	Laboratories	Laboratories	NNP
cord-299281-5z1xminb	79	51	Inc.	Inc.	NNP
cord-299281-5z1xminb	79	52	,	,	,
cord-299281-5z1xminb	79	53	Avondale	Avondale	NNP
cord-299281-5z1xminb	79	54	,	,	,
cord-299281-5z1xminb	79	55	PA	PA	NNP
cord-299281-5z1xminb	79	56	)	)	-RRB-
cord-299281-5z1xminb	79	57	.	.	.
cord-299281-5z1xminb	80	1	Cells	cell	NNS
cord-299281-5z1xminb	80	2	expressing	express	VBG
cord-299281-5z1xminb	80	3	VSV	VSV	NNP
cord-299281-5z1xminb	80	4	G	G	NNP
cord-299281-5z1xminb	80	5	or	or	CC
cord-299281-5z1xminb	80	6	mutant	mutant	JJ
cord-299281-5z1xminb	80	7	G	g	NN
cord-299281-5z1xminb	80	8	proteins	protein	NNS
cord-299281-5z1xminb	80	9	were	be	VBD
cord-299281-5z1xminb	80	10	rinsed	rinse	VBN
cord-299281-5z1xminb	80	11	once	once	RB
cord-299281-5z1xminb	80	12	with	with	IN
cord-299281-5z1xminb	80	13	PBS	PBS	NNP
cord-299281-5z1xminb	80	14	,	,	,
cord-299281-5z1xminb	80	15	starved	starve	VBN
cord-299281-5z1xminb	80	16	for	for	IN
cord-299281-5z1xminb	80	17	15	15	CD
cord-299281-5z1xminb	80	18	rain	rain	NN
cord-299281-5z1xminb	80	19	in	in	IN
cord-299281-5z1xminb	80	20	methionine	methionine	NN
cord-299281-5z1xminb	80	21	-	-	HYPH
cord-299281-5z1xminb	80	22	free	free	JJ
cord-299281-5z1xminb	80	23	DME	DME	NNP
cord-299281-5z1xminb	80	24	,	,	,
cord-299281-5z1xminb	80	25	and	and	CC
cord-299281-5z1xminb	80	26	labeled	label	VBN
cord-299281-5z1xminb	80	27	for	for	IN
cord-299281-5z1xminb	80	28	the	the	DT
cord-299281-5z1xminb	80	29	indicated	indicate	VBN
cord-299281-5z1xminb	80	30	times	time	NNS
cord-299281-5z1xminb	80	31	with	with	IN
cord-299281-5z1xminb	80	32	100	100	CD
cord-299281-5z1xminb	80	33	#	#	NN
cord-299281-5z1xminb	80	34	Ci	ci	NN
cord-299281-5z1xminb	80	35	/	/	SYM
cord-299281-5z1xminb	80	36	ml	ml	NNP
cord-299281-5z1xminb	80	37	Trans35S	Trans35S	NNP
cord-299281-5z1xminb	80	38	-	-	HYPH
cord-299281-5z1xminb	80	39	Label	Label	NNP
cord-299281-5z1xminb	80	40	(	(	-LRB-
cord-299281-5z1xminb	80	41	ICN	ICN	NNP
cord-299281-5z1xminb	80	42	Radiochemicals	Radiochemicals	NNP
cord-299281-5z1xminb	80	43	,	,	,
cord-299281-5z1xminb	80	44	Irvine	Irvine	NNP
cord-299281-5z1xminb	80	45	,	,	,
cord-299281-5z1xminb	80	46	CA	CA	NNP
cord-299281-5z1xminb	80	47	)	)	-RRB-
cord-299281-5z1xminb	80	48	or	or	CC
cord-299281-5z1xminb	80	49	L-[35S	L-[35S	NNP
cord-299281-5z1xminb	80	50	]	]	-RRB-
cord-299281-5z1xminb	81	1	in	in	IN
cord-299281-5z1xminb	81	2	vitro	vitro	FW
cord-299281-5z1xminb	81	3	cell	cell	NN
cord-299281-5z1xminb	81	4	labeling	labeling	NN
cord-299281-5z1xminb	81	5	mix	mix	NN
cord-299281-5z1xminb	82	1	(	(	-LRB-
cord-299281-5z1xminb	82	2	Amersham	Amersham	NNP
cord-299281-5z1xminb	82	3	Corp.	Corp.	NNP
cord-299281-5z1xminb	82	4	,	,	,
cord-299281-5z1xminb	82	5	Arlington	Arlington	NNP
cord-299281-5z1xminb	82	6	Heights	Heights	NNP
cord-299281-5z1xminb	82	7	,	,	,
cord-299281-5z1xminb	82	8	IL	IL	NNP
cord-299281-5z1xminb	82	9	)	)	-RRB-
cord-299281-5z1xminb	82	10	in	in	IN
cord-299281-5z1xminb	82	11	serum	serum	NN
cord-299281-5z1xminb	82	12	-	-	HYPH
cord-299281-5z1xminb	82	13	free	free	JJ
cord-299281-5z1xminb	82	14	,	,	,
cord-299281-5z1xminb	82	15	methionine	methionine	NN
cord-299281-5z1xminb	82	16	-	-	HYPH
cord-299281-5z1xminb	82	17	free	free	JJ
cord-299281-5z1xminb	82	18	DME	DME	NNP
cord-299281-5z1xminb	82	19	.	.	.
cord-299281-5z1xminb	83	1	Cells	cell	NNS
cord-299281-5z1xminb	83	2	were	be	VBD
cord-299281-5z1xminb	83	3	solubilized	solubilize	VBN
cord-299281-5z1xminb	83	4	immediately	immediately	RB
cord-299281-5z1xminb	83	5	or	or	CC
cord-299281-5z1xminb	83	6	after	after	IN
cord-299281-5z1xminb	83	7	the	the	DT
cord-299281-5z1xminb	83	8	appropriate	appropriate	JJ
cord-299281-5z1xminb	83	9	chase	chase	NN
cord-299281-5z1xminb	83	10	period	period	NN
cord-299281-5z1xminb	83	11	in	in	IN
cord-299281-5z1xminb	83	12	serum	serum	NN
cord-299281-5z1xminb	83	13	-	-	HYPH
cord-299281-5z1xminb	83	14	free	free	JJ
cord-299281-5z1xminb	83	15	DME	DME	NNP
cord-299281-5z1xminb	83	16	containing	contain	VBG
cord-299281-5z1xminb	83	17	a	a	DT
cord-299281-5z1xminb	83	18	threefold	threefold	JJ
cord-299281-5z1xminb	83	19	excess	excess	NN
cord-299281-5z1xminb	83	20	of	of	IN
cord-299281-5z1xminb	83	21	unlabeled	unlabeled	JJ
cord-299281-5z1xminb	83	22	methionine	methionine	NN
cord-299281-5z1xminb	83	23	.	.	.
cord-299281-5z1xminb	84	1	Cells	cell	NNS
cord-299281-5z1xminb	84	2	were	be	VBD
cord-299281-5z1xminb	84	3	lysed	lyse	VBN
cord-299281-5z1xminb	84	4	in	in	IN
cord-299281-5z1xminb	84	5	detergent	detergent	NN
cord-299281-5z1xminb	84	6	solution	solution	NN
cord-299281-5z1xminb	84	7	(	(	-LRB-
cord-299281-5z1xminb	84	8	50	50	CD
cord-299281-5z1xminb	84	9	mM	mm	CD
cord-299281-5z1xminb	84	10	Tris	Tris	NNP
cord-299281-5z1xminb	84	11	,	,	,
cord-299281-5z1xminb	84	12	pH	pH	NNP
cord-299281-5z1xminb	84	13	8.0	8.0	CD
cord-299281-5z1xminb	84	14	,	,	,
cord-299281-5z1xminb	84	15	1	1	CD
cord-299281-5z1xminb	84	16	%	%	NN
cord-299281-5z1xminb	84	17	NP-40	NP-40	NNP
cord-299281-5z1xminb	84	18	,	,	,
cord-299281-5z1xminb	84	19	0.4	0.4	CD
cord-299281-5z1xminb	84	20	%	%	NN
cord-299281-5z1xminb	84	21	deoxycholate	deoxycholate	NN
cord-299281-5z1xminb	84	22	,	,	,
cord-299281-5z1xminb	84	23	62.5	62.5	NNP
cord-299281-5z1xminb	84	24	mM	mM	NNP
cord-299281-5z1xminb	84	25	EDTA	EDTA	NNP
cord-299281-5z1xminb	84	26	,	,	,
cord-299281-5z1xminb	84	27	and	and	CC
cord-299281-5z1xminb	84	28	0.13	0.13	CD
cord-299281-5z1xminb	84	29	TIU	TIU	NNP
cord-299281-5z1xminb	84	30	/	/	SYM
cord-299281-5z1xminb	84	31	ml	ml	NNP
cord-299281-5z1xminb	84	32	aprotinin	aprotinin	NNP
cord-299281-5z1xminb	84	33	)	)	-RRB-
cord-299281-5z1xminb	84	34	.	.	.
cord-299281-5z1xminb	85	1	Samples	sample	NNS
cord-299281-5z1xminb	85	2	were	be	VBD
cord-299281-5z1xminb	85	3	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	85	4	using	use	VBG
cord-299281-5z1xminb	85	5	a	a	DT
cord-299281-5z1xminb	85	6	polyclonal	polyclonal	JJ
cord-299281-5z1xminb	85	7	anti	anti	JJ
cord-299281-5z1xminb	85	8	-	-	JJ
cord-299281-5z1xminb	85	9	VSV	VSV	NNP
cord-299281-5z1xminb	85	10	antibody	antibody	NN
cord-299281-5z1xminb	85	11	(	(	-LRB-
cord-299281-5z1xminb	85	12	produced	produce	VBN
cord-299281-5z1xminb	85	13	by	by	IN
cord-299281-5z1xminb	85	14	immunization	immunization	NN
cord-299281-5z1xminb	85	15	of	of	IN
cord-299281-5z1xminb	85	16	rabbits	rabbit	NNS
cord-299281-5z1xminb	85	17	with	with	IN
cord-299281-5z1xminb	85	18	purified	purify	VBN
cord-299281-5z1xminb	85	19	VSV	VSV	NNP
cord-299281-5z1xminb	85	20	)	)	-RRB-
cord-299281-5z1xminb	85	21	and	and	CC
cord-299281-5z1xminb	85	22	fixed	fix	VBN
cord-299281-5z1xminb	85	23	Staphylococcus	Staphylococcus	NNP
cord-299281-5z1xminb	85	24	aureus	aureus	NN
cord-299281-5z1xminb	85	25	(	(	-LRB-
cord-299281-5z1xminb	85	26	Calbiochem	Calbiochem	NNP
cord-299281-5z1xminb	85	27	-	-	HYPH
cord-299281-5z1xminb	85	28	Behring	Behring	NNP
cord-299281-5z1xminb	85	29	Corp.	Corp.	NNP
cord-299281-5z1xminb	85	30	,	,	,
cord-299281-5z1xminb	85	31	San	San	NNP
cord-299281-5z1xminb	85	32	Diego	Diego	NNP
cord-299281-5z1xminb	85	33	,	,	,
cord-299281-5z1xminb	85	34	CA	CA	NNP
cord-299281-5z1xminb	85	35	)	)	-RRB-
cord-299281-5z1xminb	85	36	.	.	.
cord-299281-5z1xminb	86	1	After	after	IN
cord-299281-5z1xminb	86	2	heating	heat	VBG
cord-299281-5z1xminb	86	3	to	to	IN
cord-299281-5z1xminb	86	4	100*C	100*c	CD
cord-299281-5z1xminb	86	5	for	for	IN
cord-299281-5z1xminb	86	6	3	3	CD
cord-299281-5z1xminb	86	7	rain	rain	NN
cord-299281-5z1xminb	86	8	in	in	IN
cord-299281-5z1xminb	86	9	Laemmli	Laemmli	NNP
cord-299281-5z1xminb	86	10	sample	sample	NN
cord-299281-5z1xminb	86	11	buffer	buffer	NN
cord-299281-5z1xminb	86	12	containing	contain	VBG
cord-299281-5z1xminb	86	13	5	5	CD
cord-299281-5z1xminb	86	14	%	%	NN
cord-299281-5z1xminb	86	15	fl	fl	JJ
cord-299281-5z1xminb	86	16	-	-	HYPH
cord-299281-5z1xminb	86	17	mercaptoethanol	mercaptoethanol	NNP
cord-299281-5z1xminb	86	18	,	,	,
cord-299281-5z1xminb	86	19	samples	sample	NNS
cord-299281-5z1xminb	86	20	were	be	VBD
cord-299281-5z1xminb	86	21	electrophoresed	electrophorese	VBN
cord-299281-5z1xminb	86	22	on	on	IN
cord-299281-5z1xminb	86	23	10	10	CD
cord-299281-5z1xminb	86	24	%	%	NN
cord-299281-5z1xminb	86	25	SDS	sds	NN
cord-299281-5z1xminb	86	26	-	-	HYPH
cord-299281-5z1xminb	86	27	polyacrylamide	polyacrylamide	NN
cord-299281-5z1xminb	86	28	gels	gel	NNS
cord-299281-5z1xminb	86	29	as	as	IN
cord-299281-5z1xminb	86	30	described	describe	VBN
cord-299281-5z1xminb	86	31	(	(	-LRB-
cord-299281-5z1xminb	86	32	Laemmli	Laemmli	NNP
cord-299281-5z1xminb	86	33	,	,	,
cord-299281-5z1xminb	86	34	1970	1970	CD
cord-299281-5z1xminb	86	35	)	)	-RRB-
cord-299281-5z1xminb	86	36	.	.	.
cord-299281-5z1xminb	87	1	Marker	marker	NN
cord-299281-5z1xminb	87	2	proteins	protein	NNS
cord-299281-5z1xminb	87	3	were	be	VBD
cord-299281-5z1xminb	87	4	14C	14c	NN
cord-299281-5z1xminb	87	5	-	-	HYPH
cord-299281-5z1xminb	87	6	methylated	methylate	VBN
cord-299281-5z1xminb	87	7	standard	standard	JJ
cord-299281-5z1xminb	87	8	molecular	molecular	JJ
cord-299281-5z1xminb	87	9	weight	weight	NN
cord-299281-5z1xminb	87	10	markers	marker	NNS
cord-299281-5z1xminb	87	11	(	(	-LRB-
cord-299281-5z1xminb	87	12	Amersham	Amersham	NNP
cord-299281-5z1xminb	87	13	Corp.	Corp.	NNP
cord-299281-5z1xminb	87	14	)	)	-RRB-
cord-299281-5z1xminb	87	15	.	.	.
cord-299281-5z1xminb	88	1	Labeled	label	VBN
cord-299281-5z1xminb	88	2	proteins	protein	NNS
cord-299281-5z1xminb	88	3	were	be	VBD
cord-299281-5z1xminb	88	4	detected	detect	VBN
cord-299281-5z1xminb	88	5	by	by	IN
cord-299281-5z1xminb	88	6	fluorography	fluorography	VBG
cord-299281-5z1xminb	88	7	(	(	-LRB-
cord-299281-5z1xminb	88	8	Bonner	Bonner	NNP
cord-299281-5z1xminb	88	9	and	and	CC
cord-299281-5z1xminb	88	10	Laskey	Laskey	NNP
cord-299281-5z1xminb	88	11	,	,	,
cord-299281-5z1xminb	88	12	1974	1974	CD
cord-299281-5z1xminb	88	13	)	)	-RRB-
cord-299281-5z1xminb	88	14	.	.	.
cord-299281-5z1xminb	89	1	am	be	VBP
cord-299281-5z1xminb	89	2	chimeras	chimera	NNS
cord-299281-5z1xminb	89	3	were	be	VBD
cord-299281-5z1xminb	89	4	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	89	5	and	and	CC
cord-299281-5z1xminb	89	6	solubilized	solubilize	VBN
cord-299281-5z1xminb	89	7	as	as	IN
cord-299281-5z1xminb	89	8	above	above	RB
cord-299281-5z1xminb	89	9	but	but	CC
cord-299281-5z1xminb	89	10	immunoprecipitated	immunoprecipitated	JJ
cord-299281-5z1xminb	89	11	using	use	VBG
cord-299281-5z1xminb	89	12	anti	anti	JJ
cord-299281-5z1xminb	89	13	-	-	JJ
cord-299281-5z1xminb	89	14	human	human	JJ
cord-299281-5z1xminb	89	15	chorionic	chorionic	JJ
cord-299281-5z1xminb	89	16	gonadotropin	gonadotropin	NN
cord-299281-5z1xminb	89	17	(	(	-LRB-
cord-299281-5z1xminb	89	18	hCG	hCG	NNP
cord-299281-5z1xminb	89	19	)	)	-RRB-
cord-299281-5z1xminb	89	20	antibody	antibody	NN
cord-299281-5z1xminb	89	21	(	(	-LRB-
cord-299281-5z1xminb	89	22	Organon	Organon	NNP
cord-299281-5z1xminb	89	23	Teknika	Teknika	NNP
cord-299281-5z1xminb	89	24	-	-	HYPH
cord-299281-5z1xminb	89	25	Cappel	Cappel	NNP
cord-299281-5z1xminb	89	26	,	,	,
cord-299281-5z1xminb	89	27	West	West	NNP
cord-299281-5z1xminb	89	28	Chester	Chester	NNP
cord-299281-5z1xminb	89	29	,	,	,
cord-299281-5z1xminb	89	30	PA	PA	NNP
cord-299281-5z1xminb	89	31	)	)	-RRB-
cord-299281-5z1xminb	89	32	and	and	CC
cord-299281-5z1xminb	89	33	electrophoresed	electrophorese	VBD
cord-299281-5z1xminb	89	34	on	on	IN
cord-299281-5z1xminb	89	35	12	12	CD
cord-299281-5z1xminb	89	36	%	%	NN
cord-299281-5z1xminb	89	37	SDS	sds	NN
cord-299281-5z1xminb	89	38	-	-	HYPH
cord-299281-5z1xminb	89	39	pelyacrylamide	pelyacrylamide	NN
cord-299281-5z1xminb	89	40	gels	gel	NNS
cord-299281-5z1xminb	89	41	.	.	.
cord-299281-5z1xminb	90	1	Brefeldin	Brefeldin	NNP
cord-299281-5z1xminb	90	2	A	A	NNP
cord-299281-5z1xminb	90	3	(	(	-LRB-
cord-299281-5z1xminb	90	4	5	5	CD
cord-299281-5z1xminb	90	5	/tg	/tg	NNP
cord-299281-5z1xminb	90	6	/	/	SYM
cord-299281-5z1xminb	90	7	ml	ml	NN
cord-299281-5z1xminb	90	8	final	final	JJ
cord-299281-5z1xminb	90	9	concentration	concentration	NN
cord-299281-5z1xminb	90	10	)	)	-RRB-
cord-299281-5z1xminb	90	11	was	be	VBD
cord-299281-5z1xminb	90	12	diluted	dilute	VBN
cord-299281-5z1xminb	90	13	into	into	IN
cord-299281-5z1xminb	90	14	serum	serum	NN
cord-299281-5z1xminb	90	15	-	-	HYPH
cord-299281-5z1xminb	90	16	free	free	JJ
cord-299281-5z1xminb	90	17	medium	medium	NN
cord-299281-5z1xminb	90	18	from	from	IN
cord-299281-5z1xminb	90	19	a	a	DT
cord-299281-5z1xminb	90	20	5	5	CD
cord-299281-5z1xminb	90	21	mg	mg	NNP
cord-299281-5z1xminb	90	22	/	/	SYM
cord-299281-5z1xminb	90	23	ml	ml	NN
cord-299281-5z1xminb	90	24	stock	stock	NN
cord-299281-5z1xminb	90	25	in	in	IN
cord-299281-5z1xminb	90	26	ethanol	ethanol	NN
cord-299281-5z1xminb	90	27	immediately	immediately	RB
cord-299281-5z1xminb	90	28	before	before	IN
cord-299281-5z1xminb	90	29	use	use	NN
cord-299281-5z1xminb	90	30	.	.	.
cord-299281-5z1xminb	91	1	Colehicine	Colehicine	NNP
cord-299281-5z1xminb	91	2	(	(	-LRB-
cord-299281-5z1xminb	91	3	25	25	CD
cord-299281-5z1xminb	91	4	mM	mm	CD
cord-299281-5z1xminb	91	5	)	)	-RRB-
cord-299281-5z1xminb	91	6	was	be	VBD
cord-299281-5z1xminb	91	7	prepared	prepare	VBN
cord-299281-5z1xminb	91	8	in	in	IN
cord-299281-5z1xminb	91	9	DMSO	DMSO	NNP
cord-299281-5z1xminb	91	10	,	,	,
cord-299281-5z1xminb	91	11	diluted	dilute	VBN
cord-299281-5z1xminb	91	12	1:10	1:10	CD
cord-299281-5z1xminb	91	13	into	into	IN
cord-299281-5z1xminb	91	14	serum	serum	NN
cord-299281-5z1xminb	91	15	-	-	HYPH
cord-299281-5z1xminb	91	16	free	free	JJ
cord-299281-5z1xminb	91	17	,	,	,
cord-299281-5z1xminb	91	18	methionine	methionine	NN
cord-299281-5z1xminb	91	19	-	-	HYPH
cord-299281-5z1xminb	91	20	free	free	JJ
cord-299281-5z1xminb	91	21	medium	medium	NN
cord-299281-5z1xminb	91	22	,	,	,
cord-299281-5z1xminb	91	23	and	and	CC
cord-299281-5z1xminb	91	24	added	add	VBD
cord-299281-5z1xminb	91	25	to	to	IN
cord-299281-5z1xminb	91	26	cells	cell	NNS
cord-299281-5z1xminb	91	27	(	(	-LRB-
cord-299281-5z1xminb	91	28	50	50	CD
cord-299281-5z1xminb	91	29	~tM	~tM	NFP
cord-299281-5z1xminb	91	30	final	final	JJ
cord-299281-5z1xminb	91	31	concentration	concentration	NN
cord-299281-5z1xminb	91	32	)	)	-RRB-
cord-299281-5z1xminb	91	33	starting	start	VBG
cord-299281-5z1xminb	91	34	1	1	CD
cord-299281-5z1xminb	91	35	h	h	NN
cord-299281-5z1xminb	91	36	before	before	IN
cord-299281-5z1xminb	91	37	metabolic	metabolic	JJ
cord-299281-5z1xminb	91	38	labeling	labeling	NN
cord-299281-5z1xminb	91	39	.	.	.
cord-299281-5z1xminb	92	1	Cytochalasin	Cytochalasin	NNP
cord-299281-5z1xminb	92	2	D	D	NNP
cord-299281-5z1xminb	92	3	(	(	-LRB-
cord-299281-5z1xminb	92	4	0.1	0.1	CD
cord-299281-5z1xminb	92	5	M	m	NN
cord-299281-5z1xminb	92	6	in	in	IN
cord-299281-5z1xminb	92	7	DMSO	DMSO	NNP
cord-299281-5z1xminb	92	8	)	)	-RRB-
cord-299281-5z1xminb	92	9	was	be	VBD
cord-299281-5z1xminb	92	10	added	add	VBN
cord-299281-5z1xminb	92	11	to	to	IN
cord-299281-5z1xminb	92	12	cells	cell	NNS
cord-299281-5z1xminb	92	13	(	(	-LRB-
cord-299281-5z1xminb	92	14	100/~M	100/~m	CD
cord-299281-5z1xminb	92	15	final	final	JJ
cord-299281-5z1xminb	92	16	concentration	concentration	NN
cord-299281-5z1xminb	92	17	)	)	-RRB-
cord-299281-5z1xminb	92	18	2	2	CD
cord-299281-5z1xminb	92	19	h	h	NN
cord-299281-5z1xminb	92	20	after	after	IN
cord-299281-5z1xminb	92	21	infection	infection	NN
cord-299281-5z1xminb	92	22	and	and	CC
cord-299281-5z1xminb	92	23	ineluded	inelude	VBN
cord-299281-5z1xminb	92	24	in	in	IN
cord-299281-5z1xminb	92	25	subsequent	subsequent	JJ
cord-299281-5z1xminb	92	26	changes	change	NNS
cord-299281-5z1xminb	92	27	of	of	IN
cord-299281-5z1xminb	92	28	medium	medium	NN
cord-299281-5z1xminb	92	29	.	.	.
cord-299281-5z1xminb	93	1	An	an	DT
cord-299281-5z1xminb	93	2	equal	equal	JJ
cord-299281-5z1xminb	93	3	volume	volume	NN
cord-299281-5z1xminb	93	4	of	of	IN
cord-299281-5z1xminb	93	5	DMSO	DMSO	NNP
cord-299281-5z1xminb	93	6	was	be	VBD
cord-299281-5z1xminb	93	7	added	add	VBN
cord-299281-5z1xminb	93	8	to	to	TO
cord-299281-5z1xminb	93	9	control	control	VB
cord-299281-5z1xminb	93	10	cells	cell	NNS
cord-299281-5z1xminb	93	11	.	.	.
cord-299281-5z1xminb	94	1	In	in	IN
cord-299281-5z1xminb	94	2	experiments	experiment	NNS
cord-299281-5z1xminb	94	3	involving	involve	VBG
cord-299281-5z1xminb	94	4	cell	cell	NN
cord-299281-5z1xminb	94	5	treatment	treatment	NN
cord-299281-5z1xminb	94	6	with	with	IN
cord-299281-5z1xminb	94	7	CCCP	CCCP	NNP
cord-299281-5z1xminb	94	8	,	,	,
cord-299281-5z1xminb	94	9	ceils	ceil	NNS
cord-299281-5z1xminb	94	10	were	be	VBD
cord-299281-5z1xminb	94	11	starved	starve	VBN
cord-299281-5z1xminb	94	12	in	in	IN
cord-299281-5z1xminb	94	13	glucose-	glucose-	NN
cord-299281-5z1xminb	94	14	,	,	,
cord-299281-5z1xminb	94	15	methionine-	methionine-	NNP
cord-299281-5z1xminb	94	16	,	,	,
cord-299281-5z1xminb	94	17	and	and	CC
cord-299281-5z1xminb	94	18	cysteine	cysteine	NN
cord-299281-5z1xminb	94	19	-	-	HYPH
cord-299281-5z1xminb	94	20	free	free	JJ
cord-299281-5z1xminb	94	21	medium	medium	NN
cord-299281-5z1xminb	94	22	for	for	IN
cord-299281-5z1xminb	94	23	15	15	CD
cord-299281-5z1xminb	94	24	rain	rain	NN
cord-299281-5z1xminb	94	25	before	before	IN
cord-299281-5z1xminb	94	26	metabolic	metabolic	JJ
cord-299281-5z1xminb	94	27	labeling	labeling	NN
cord-299281-5z1xminb	94	28	in	in	IN
cord-299281-5z1xminb	94	29	the	the	DT
cord-299281-5z1xminb	94	30	same	same	JJ
cord-299281-5z1xminb	94	31	medium	medium	NN
cord-299281-5z1xminb	94	32	for	for	IN
cord-299281-5z1xminb	94	33	5	5	CD
cord-299281-5z1xminb	94	34	win	win	NN
cord-299281-5z1xminb	94	35	.	.	.
cord-299281-5z1xminb	95	1	CCCP	CCCP	NNP
cord-299281-5z1xminb	95	2	(	(	-LRB-
cord-299281-5z1xminb	95	3	0.1	0.1	CD
cord-299281-5z1xminb	95	4	M	m	NN
cord-299281-5z1xminb	95	5	stock	stock	NN
cord-299281-5z1xminb	95	6	in	in	IN
cord-299281-5z1xminb	95	7	DMSO	DMSO	NNP
cord-299281-5z1xminb	95	8	)	)	-RRB-
cord-299281-5z1xminb	95	9	was	be	VBD
cord-299281-5z1xminb	95	10	added	add	VBN
cord-299281-5z1xminb	95	11	at	at	IN
cord-299281-5z1xminb	95	12	the	the	DT
cord-299281-5z1xminb	95	13	beginning	beginning	NN
cord-299281-5z1xminb	95	14	of	of	IN
cord-299281-5z1xminb	95	15	the	the	DT
cord-299281-5z1xminb	95	16	chase	chase	NNP
cord-299281-5z1xminb	95	17	in	in	IN
cord-299281-5z1xminb	95	18	glucosefree	glucosefree	NNP
cord-299281-5z1xminb	95	19	medium	medium	NN
cord-299281-5z1xminb	95	20	to	to	IN
cord-299281-5z1xminb	95	21	a	a	DT
cord-299281-5z1xminb	95	22	final	final	JJ
cord-299281-5z1xminb	95	23	concentration	concentration	NN
cord-299281-5z1xminb	95	24	of	of	IN
cord-299281-5z1xminb	95	25	300	300	CD
cord-299281-5z1xminb	95	26	#	#	NN
cord-299281-5z1xminb	95	27	M.	M.	NNP
cord-299281-5z1xminb	95	28	HeLa	HeLa	NNP
cord-299281-5z1xminb	95	29	cells	cell	NNS
cord-299281-5z1xminb	95	30	were	be	VBD
cord-299281-5z1xminb	95	31	transfected	transfecte	VBN
cord-299281-5z1xminb	95	32	,	,	,
cord-299281-5z1xminb	95	33	metabolically	metabolically	RB
cord-299281-5z1xminb	95	34	labeled	label	VBN
cord-299281-5z1xminb	95	35	for	for	IN
cord-299281-5z1xminb	95	36	15	15	CD
cord-299281-5z1xminb	95	37	min	min	NN
cord-299281-5z1xminb	95	38	,	,	,
cord-299281-5z1xminb	95	39	and	and	CC
cord-299281-5z1xminb	95	40	chased	chase	VBD
cord-299281-5z1xminb	95	41	for	for	IN
cord-299281-5z1xminb	95	42	45	45	CD
cord-299281-5z1xminb	95	43	rain	rain	NN
cord-299281-5z1xminb	95	44	as	as	IN
cord-299281-5z1xminb	95	45	described	describe	VBN
cord-299281-5z1xminb	95	46	above	above	RB
cord-299281-5z1xminb	95	47	.	.	.
cord-299281-5z1xminb	96	1	Cells	cell	NNS
cord-299281-5z1xminb	96	2	were	be	VBD
cord-299281-5z1xminb	96	3	rinsed	rinse	VBN
cord-299281-5z1xminb	96	4	three	three	CD
cord-299281-5z1xminb	96	5	times	time	NNS
cord-299281-5z1xminb	96	6	in	in	IN
cord-299281-5z1xminb	96	7	PBS	PBS	NNP
cord-299281-5z1xminb	96	8	supplemented	supplement	VBN
cord-299281-5z1xminb	96	9	with	with	IN
cord-299281-5z1xminb	96	10	1	1	CD
cord-299281-5z1xminb	96	11	lnM	lnM	NNS
cord-299281-5z1xminb	96	12	MgCI2	MgCI2	NNP
cord-299281-5z1xminb	96	13	and	and	CC
cord-299281-5z1xminb	96	14	0.1	0.1	CD
cord-299281-5z1xminb	96	15	mM	mm	CD
cord-299281-5z1xminb	96	16	CaC12	cac12	NN
cord-299281-5z1xminb	96	17	,	,	,
cord-299281-5z1xminb	96	18	and	and	CC
cord-299281-5z1xminb	96	19	then	then	RB
cord-299281-5z1xminb	96	20	biotinylated	biotinylate	VBD
cord-299281-5z1xminb	96	21	using	use	VBG
cord-299281-5z1xminb	96	22	sulfo	sulfo	NNP
cord-299281-5z1xminb	96	23	-	-	HYPH
cord-299281-5z1xminb	96	24	NHS	NHS	NNP
cord-299281-5z1xminb	96	25	-	-	HYPH
cord-299281-5z1xminb	96	26	biotin	biotin	NNP
cord-299281-5z1xminb	96	27	(	(	-LRB-
cord-299281-5z1xminb	96	28	Pierce	Pierce	NNP
cord-299281-5z1xminb	96	29	Chem	Chem	NNP
cord-299281-5z1xminb	96	30	.	.	.
cord-299281-5z1xminb	97	1	Co.	Co.	NNP
cord-299281-5z1xminb	97	2	,	,	,
cord-299281-5z1xminb	97	3	Rockford	Rockford	NNP
cord-299281-5z1xminb	97	4	,	,	,
cord-299281-5z1xminb	97	5	IL	IL	NNP
cord-299281-5z1xminb	97	6	)	)	-RRB-
cord-299281-5z1xminb	97	7	as	as	IN
cord-299281-5z1xminb	97	8	described	describe	VBN
cord-299281-5z1xminb	97	9	(	(	-LRB-
cord-299281-5z1xminb	97	10	Lisanti	Lisanti	NNP
cord-299281-5z1xminb	97	11	et	et	NNP
cord-299281-5z1xminb	97	12	al	al	NNP
cord-299281-5z1xminb	97	13	.	.	NNP
cord-299281-5z1xminb	97	14	,	,	,
cord-299281-5z1xminb	97	15	1988	1988	CD
cord-299281-5z1xminb	97	16	)	)	-RRB-
cord-299281-5z1xminb	97	17	.	.	.
cord-299281-5z1xminb	98	1	Cells	cell	NNS
cord-299281-5z1xminb	98	2	were	be	VBD
cord-299281-5z1xminb	98	3	then	then	RB
cord-299281-5z1xminb	98	4	solubilized	solubilize	VBN
cord-299281-5z1xminb	98	5	as	as	IN
cord-299281-5z1xminb	98	6	described	describe	VBN
cord-299281-5z1xminb	98	7	above	above	RB
cord-299281-5z1xminb	98	8	and	and	CC
cord-299281-5z1xminb	98	9	Gml	Gml	NNP
cord-299281-5z1xminb	98	10	and	and	CC
cord-299281-5z1xminb	98	11	related	related	JJ
cord-299281-5z1xminb	98	12	proteins	protein	NNS
cord-299281-5z1xminb	98	13	immunoprecipitated	immunoprecipitate	VBD
cord-299281-5z1xminb	98	14	.	.	.
cord-299281-5z1xminb	99	1	Samples	sample	NNS
cord-299281-5z1xminb	99	2	were	be	VBD
cord-299281-5z1xminb	99	3	eluted	elute	VBN
cord-299281-5z1xminb	99	4	from	from	IN
cord-299281-5z1xminb	99	5	the	the	DT
cord-299281-5z1xminb	99	6	S.	S.	NNP
cord-299281-5z1xminb	99	7	aureus	aureus	NN
cord-299281-5z1xminb	99	8	pellets	pellet	NNS
cord-299281-5z1xminb	99	9	by	by	IN
cord-299281-5z1xminb	99	10	heating	heat	VBG
cord-299281-5z1xminb	99	11	to	to	IN
cord-299281-5z1xminb	99	12	100	100	CD
cord-299281-5z1xminb	99	13	°	°	NN
cord-299281-5z1xminb	99	14	C	c	NN
cord-299281-5z1xminb	99	15	in	in	IN
cord-299281-5z1xminb	99	16	50/~1	50/~1	CD
cord-299281-5z1xminb	99	17	10	10	CD
cord-299281-5z1xminb	99	18	mM	mm	CD
cord-299281-5z1xminb	99	19	"	"	``
cord-299281-5z1xminb	99	20	Iris	Iris	NNP
cord-299281-5z1xminb	99	21	,	,	,
cord-299281-5z1xminb	99	22	50	50	CD
cord-299281-5z1xminb	99	23	mM	mm	CD
cord-299281-5z1xminb	99	24	NaC1	NaC1	NNPS
cord-299281-5z1xminb	99	25	,	,	,
cord-299281-5z1xminb	99	26	1	1	CD
cord-299281-5z1xminb	99	27	%	%	NN
cord-299281-5z1xminb	99	28	SDS	SDS	NNP
cord-299281-5z1xminb	99	29	,	,	,
cord-299281-5z1xminb	99	30	pH	pH	NNP
cord-299281-5z1xminb	99	31	7.4	7.4	CD
cord-299281-5z1xminb	99	32	,	,	,
cord-299281-5z1xminb	99	33	and	and	CC
cord-299281-5z1xminb	99	34	divided	divide	VBD
cord-299281-5z1xminb	99	35	into	into	IN
cord-299281-5z1xminb	99	36	two	two	CD
cord-299281-5z1xminb	99	37	equal	equal	JJ
cord-299281-5z1xminb	99	38	aliquots	aliquot	NNS
cord-299281-5z1xminb	99	39	.	.	.
cord-299281-5z1xminb	100	1	One	one	CD
cord-299281-5z1xminb	100	2	aliquot	aliquot	NN
cord-299281-5z1xminb	100	3	was	be	VBD
cord-299281-5z1xminb	100	4	kept	keep	VBN
cord-299281-5z1xminb	100	5	at	at	IN
cord-299281-5z1xminb	100	6	-20	-20	NNP
cord-299281-5z1xminb	100	7	°	°	NNP
cord-299281-5z1xminb	100	8	C	C	NNP
cord-299281-5z1xminb	100	9	(	(	-LRB-
cord-299281-5z1xminb	100	10	"	"	``
cord-299281-5z1xminb	100	11	total	total	JJ
cord-299281-5z1xminb	100	12	"	"	''
cord-299281-5z1xminb	100	13	)	)	-RRB-
cord-299281-5z1xminb	100	14	while	while	IN
cord-299281-5z1xminb	100	15	the	the	DT
cord-299281-5z1xminb	100	16	other	other	JJ
cord-299281-5z1xminb	100	17	was	be	VBD
cord-299281-5z1xminb	100	18	diluted	dilute	VBN
cord-299281-5z1xminb	100	19	tenfold	tenfold	RB
cord-299281-5z1xminb	100	20	with	with	IN
cord-299281-5z1xminb	100	21	10	10	CD
cord-299281-5z1xminb	100	22	mM	mm	CD
cord-299281-5z1xminb	100	23	Tris	Tris	NNP
cord-299281-5z1xminb	100	24	,	,	,
cord-299281-5z1xminb	100	25	50	50	CD
cord-299281-5z1xminb	100	26	mM	mm	CD
cord-299281-5z1xminb	100	27	NaCI	naci	NN
cord-299281-5z1xminb	100	28	,	,	,
cord-299281-5z1xminb	100	29	pH	pH	NNP
cord-299281-5z1xminb	100	30	7.4	7.4	CD
cord-299281-5z1xminb	100	31	,	,	,
cord-299281-5z1xminb	100	32	and	and	CC
cord-299281-5z1xminb	100	33	incubated	incubate	VBN
cord-299281-5z1xminb	100	34	with	with	IN
cord-299281-5z1xminb	100	35	streptavidin	streptavidin	NN
cord-299281-5z1xminb	100	36	-	-	HYPH
cord-299281-5z1xminb	100	37	coupled	couple	VBN
cord-299281-5z1xminb	100	38	agarose	agarose	NN
cord-299281-5z1xminb	100	39	beads	bead	NNS
cord-299281-5z1xminb	100	40	(	(	-LRB-
cord-299281-5z1xminb	100	41	Pierce	Pierce	NNP
cord-299281-5z1xminb	100	42	Chem	Chem	NNP
cord-299281-5z1xminb	100	43	.	.	.
cord-299281-5z1xminb	100	44	Co.	Co.	NNP
cord-299281-5z1xminb	100	45	)	)	-RRB-
cord-299281-5z1xminb	100	46	for	for	IN
cord-299281-5z1xminb	100	47	60	60	CD
cord-299281-5z1xminb	100	48	min	min	NN
cord-299281-5z1xminb	100	49	at	at	IN
cord-299281-5z1xminb	100	50	4"C.	4"c.	CD
cord-299281-5z1xminb	101	1	The	the	DT
cord-299281-5z1xminb	101	2	streptavidin	streptavidin	JJ
cord-299281-5z1xminb	101	3	beads	bead	NNS
cord-299281-5z1xminb	101	4	were	be	VBD
cord-299281-5z1xminb	101	5	then	then	RB
cord-299281-5z1xminb	101	6	pelleted	pellete	VBN
cord-299281-5z1xminb	101	7	and	and	CC
cord-299281-5z1xminb	101	8	washed	wash	VBD
cord-299281-5z1xminb	101	9	three	three	CD
cord-299281-5z1xminb	101	10	times	time	NNS
cord-299281-5z1xminb	101	11	in	in	IN
cord-299281-5z1xminb	101	12	RIPA	RIPA	NNP
cord-299281-5z1xminb	101	13	buffer	buffer	NN
cord-299281-5z1xminb	101	14	.	.	.
cord-299281-5z1xminb	102	1	Non	non	JJ
cord-299281-5z1xminb	102	2	-	-	JJ
cord-299281-5z1xminb	102	3	biotinylated	biotinylated	JJ
cord-299281-5z1xminb	102	4	proteins	protein	NNS
cord-299281-5z1xminb	102	5	in	in	IN
cord-299281-5z1xminb	102	6	the	the	DT
cord-299281-5z1xminb	102	7	supernatant	supernatant	NN
cord-299281-5z1xminb	102	8	were	be	VBD
cord-299281-5z1xminb	102	9	precipitated	precipitate	VBN
cord-299281-5z1xminb	102	10	using	use	VBG
cord-299281-5z1xminb	102	11	"	"	``
cord-299281-5z1xminb	102	12	I~	I~	NNP
cord-299281-5z1xminb	103	1	A.	A.	NNP
cord-299281-5z1xminb	103	2	Samples	sample	NNS
cord-299281-5z1xminb	103	3	were	be	VBD
cord-299281-5z1xminb	103	4	analyzed	analyze	VBN
cord-299281-5z1xminb	103	5	by	by	IN
cord-299281-5z1xminb	103	6	SDS	SDS	NNP
cord-299281-5z1xminb	103	7	-	-	HYPH
cord-299281-5z1xminb	103	8	PAGE	PAGE	NNP
cord-299281-5z1xminb	103	9	followed	follow	VBN
cord-299281-5z1xminb	103	10	by	by	IN
cord-299281-5z1xminb	103	11	fluorography	fluorography	NNP
cord-299281-5z1xminb	103	12	.	.	.
cord-299281-5z1xminb	104	1	Oligomerization	Oligomerization	NNP
cord-299281-5z1xminb	104	2	of	of	IN
cord-299281-5z1xminb	104	3	the	the	DT
cord-299281-5z1xminb	104	4	Gml	Gml	NNP
cord-299281-5z1xminb	104	5	protein	protein	NN
cord-299281-5z1xminb	104	6	was	be	VBD
cord-299281-5z1xminb	104	7	analyzed	analyze	VBN
cord-299281-5z1xminb	104	8	by	by	IN
cord-299281-5z1xminb	104	9	velocity	velocity	NN
cord-299281-5z1xminb	104	10	gradient	gradient	NN
cord-299281-5z1xminb	104	11	centrifugetion	centrifugetion	NN
cord-299281-5z1xminb	104	12	in	in	IN
cord-299281-5z1xminb	104	13	sucrose	sucrose	NN
cord-299281-5z1xminb	104	14	performed	perform	VBN
cord-299281-5z1xminb	104	15	essentially	essentially	RB
cord-299281-5z1xminb	104	16	as	as	IN
cord-299281-5z1xminb	104	17	described	describe	VBN
cord-299281-5z1xminb	104	18	(	(	-LRB-
cord-299281-5z1xminb	104	19	Doms	Doms	NNP
cord-299281-5z1xminb	104	20	et	et	FW
cord-299281-5z1xminb	104	21	al	al	NNP
cord-299281-5z1xminb	104	22	.	.	NNP
cord-299281-5z1xminb	104	23	,	,	,
cord-299281-5z1xminb	104	24	1987	1987	CD
cord-299281-5z1xminb	104	25	)	)	-RRB-
cord-299281-5z1xminb	104	26	.	.	.
cord-299281-5z1xminb	105	1	Continuous	continuous	JJ
cord-299281-5z1xminb	105	2	5	5	CD
cord-299281-5z1xminb	105	3	-	-	SYM
cord-299281-5z1xminb	105	4	20	20	CD
cord-299281-5z1xminb	105	5	%	%	NN
cord-299281-5z1xminb	105	6	(	(	-LRB-
cord-299281-5z1xminb	105	7	wt	wt	NN
cord-299281-5z1xminb	105	8	/	/	SYM
cord-299281-5z1xminb	105	9	wt	wt	NN
cord-299281-5z1xminb	105	10	)	)	-RRB-
cord-299281-5z1xminb	106	1	sucrose	sucrose	NN
cord-299281-5z1xminb	106	2	gradients	gradient	NNS
cord-299281-5z1xminb	106	3	were	be	VBD
cord-299281-5z1xminb	106	4	poured	pour	VBN
cord-299281-5z1xminb	106	5	over	over	RP
cord-299281-5z1xminb	106	6	a	a	DT
cord-299281-5z1xminb	106	7	60	60	CD
cord-299281-5z1xminb	106	8	%	%	NN
cord-299281-5z1xminb	106	9	(	(	-LRB-
cord-299281-5z1xminb	106	10	wt	wt	NN
cord-299281-5z1xminb	106	11	/	/	SYM
cord-299281-5z1xminb	106	12	wt	wt	NN
cord-299281-5z1xminb	106	13	)	)	-RRB-
cord-299281-5z1xminb	106	14	sucrose	sucrose	NN
cord-299281-5z1xminb	106	15	cushion	cushion	NN
cord-299281-5z1xminb	106	16	(	(	-LRB-
cord-299281-5z1xminb	106	17	0.4	0.4	CD
cord-299281-5z1xminb	106	18	nil	nil	NN
cord-299281-5z1xminb	106	19	)	)	-RRB-
cord-299281-5z1xminb	106	20	in	in	IN
cord-299281-5z1xminb	106	21	SW50.1	sw50.1	JJ
cord-299281-5z1xminb	106	22	tubes	tube	NNS
cord-299281-5z1xminb	106	23	.	.	.
cord-299281-5z1xminb	107	1	All	all	DT
cord-299281-5z1xminb	107	2	solutions	solution	NNS
cord-299281-5z1xminb	107	3	were	be	VBD
cord-299281-5z1xminb	107	4	in	in	IN
cord-299281-5z1xminb	107	5	MNT	MNT	NNP
cord-299281-5z1xminb	107	6	(	(	-LRB-
cord-299281-5z1xminb	107	7	100	100	CD
cord-299281-5z1xminb	107	8	mM	mm	CD
cord-299281-5z1xminb	107	9	NaCI	naci	NN
cord-299281-5z1xminb	107	10	,	,	,
cord-299281-5z1xminb	107	11	20	20	CD
cord-299281-5z1xminb	107	12	mM	mM	NNP
cord-299281-5z1xminb	107	13	Tris	Tris	NNP
cord-299281-5z1xminb	107	14	,	,	,
cord-299281-5z1xminb	107	15	30	30	CD
cord-299281-5z1xminb	107	16	mM	mm	CD
cord-299281-5z1xminb	107	17	MES	MES	NNP
cord-299281-5z1xminb	107	18	,	,	,
cord-299281-5z1xminb	107	19	pH	pH	NNP
cord-299281-5z1xminb	107	20	5.8	5.8	CD
cord-299281-5z1xminb	107	21	)	)	-RRB-
cord-299281-5z1xminb	107	22	containing	contain	VBG
cord-299281-5z1xminb	107	23	0.1	0.1	CD
cord-299281-5z1xminb	107	24	%	%	NN
cord-299281-5z1xminb	107	25	Triton	Triton	NNP
cord-299281-5z1xminb	107	26	X-100	x-100	RB
cord-299281-5z1xminb	107	27	.	.	.
cord-299281-5z1xminb	108	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	108	2	cells	cell	NNS
cord-299281-5z1xminb	108	3	expressing	express	VBG
cord-299281-5z1xminb	108	4	either	either	CC
cord-299281-5z1xminb	108	5	VSV	VSV	NNP
cord-299281-5z1xminb	108	6	G	G	NNP
cord-299281-5z1xminb	108	7	or	or	CC
cord-299281-5z1xminb	108	8	Gml	Gml	NNP
cord-299281-5z1xminb	108	9	were	be	VBD
cord-299281-5z1xminb	108	10	metabolically	metabolically	RB
cord-299281-5z1xminb	108	11	labeled	label	VBN
cord-299281-5z1xminb	108	12	for	for	IN
cord-299281-5z1xminb	108	13	5	5	CD
cord-299281-5z1xminb	108	14	min	min	NN
cord-299281-5z1xminb	108	15	and	and	CC
cord-299281-5z1xminb	108	16	harvested	harvest	VBN
cord-299281-5z1xminb	108	17	(	(	-LRB-
cord-299281-5z1xminb	108	18	in	in	IN
cord-299281-5z1xminb	108	19	MNT	mnt	NN
cord-299281-5z1xminb	108	20	containing	contain	VBG
cord-299281-5z1xminb	108	21	1	1	CD
cord-299281-5z1xminb	108	22	%	%	NN
cord-299281-5z1xminb	108	23	Triton	Triton	NNP
cord-299281-5z1xminb	108	24	X-100	X-100	NNS
cord-299281-5z1xminb	108	25	)	)	-RRB-
cord-299281-5z1xminb	108	26	either	either	CC
cord-299281-5z1xminb	108	27	immediately	immediately	RB
cord-299281-5z1xminb	108	28	or	or	CC
cord-299281-5z1xminb	108	29	after	after	IN
cord-299281-5z1xminb	108	30	60	60	CD
cord-299281-5z1xminb	108	31	min	min	NN
cord-299281-5z1xminb	108	32	of	of	IN
cord-299281-5z1xminb	108	33	chase	chase	NN
cord-299281-5z1xminb	108	34	.	.	.
cord-299281-5z1xminb	109	1	Lysates	Lysates	NNP
cord-299281-5z1xminb	109	2	were	be	VBD
cord-299281-5z1xminb	109	3	loaded	load	VBN
cord-299281-5z1xminb	109	4	on	on	IN
cord-299281-5z1xminb	109	5	top	top	NN
cord-299281-5z1xminb	109	6	of	of	IN
cord-299281-5z1xminb	109	7	the	the	DT
cord-299281-5z1xminb	109	8	gradients	gradient	NNS
cord-299281-5z1xminb	109	9	and	and	CC
cord-299281-5z1xminb	109	10	spun	spin	VBD
cord-299281-5z1xminb	109	11	at	at	IN
cord-299281-5z1xminb	109	12	44	44	CD
cord-299281-5z1xminb	109	13	-	-	SYM
cord-299281-5z1xminb	109	14	45,000	45,000	CD
cord-299281-5z1xminb	109	15	rpm	rpm	NN
cord-299281-5z1xminb	109	16	for	for	IN
cord-299281-5z1xminb	109	17	15	15	CD
cord-299281-5z1xminb	109	18	-	-	SYM
cord-299281-5z1xminb	109	19	16	16	CD
cord-299281-5z1xminb	109	20	h.	h.	NNP
cord-299281-5z1xminb	109	21	Fractions	Fractions	NNPS
cord-299281-5z1xminb	109	22	(	(	-LRB-
cord-299281-5z1xminb	109	23	0.35	0.35	CD
cord-299281-5z1xminb	109	24	ml	ml	NN
cord-299281-5z1xminb	109	25	)	)	-RRB-
cord-299281-5z1xminb	109	26	were	be	VBD
cord-299281-5z1xminb	109	27	collected	collect	VBN
cord-299281-5z1xminb	109	28	from	from	IN
cord-299281-5z1xminb	109	29	the	the	DT
cord-299281-5z1xminb	109	30	top	top	NN
cord-299281-5z1xminb	109	31	using	use	VBG
cord-299281-5z1xminb	109	32	a	a	DT
cord-299281-5z1xminb	109	33	Buchler	Buchler	NNP
cord-299281-5z1xminb	109	34	Auto	Auto	NNP
cord-299281-5z1xminb	109	35	Densi	Densi	NNP
cord-299281-5z1xminb	109	36	-	-	HYPH
cord-299281-5z1xminb	109	37	Flow	Flow	NNP
cord-299281-5z1xminb	109	38	IIC	IIC	NNP
cord-299281-5z1xminb	109	39	,	,	,
cord-299281-5z1xminb	109	40	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	109	41	with	with	IN
cord-299281-5z1xminb	109	42	anti	anti	JJ
cord-299281-5z1xminb	109	43	-	-	JJ
cord-299281-5z1xminb	109	44	VSV	VSV	NNP
cord-299281-5z1xminb	109	45	antibody	antibody	NN
cord-299281-5z1xminb	109	46	,	,	,
cord-299281-5z1xminb	109	47	and	and	CC
cord-299281-5z1xminb	109	48	electrophoresed	electrophorese	VBD
cord-299281-5z1xminb	109	49	to	to	TO
cord-299281-5z1xminb	109	50	determine	determine	VB
cord-299281-5z1xminb	109	51	the	the	DT
cord-299281-5z1xminb	109	52	location	location	NN
cord-299281-5z1xminb	109	53	of	of	IN
cord-299281-5z1xminb	109	54	the	the	DT
cord-299281-5z1xminb	109	55	proteins	protein	NNS
cord-299281-5z1xminb	109	56	in	in	IN
cord-299281-5z1xminb	109	57	the	the	DT
cord-299281-5z1xminb	109	58	gradient	gradient	NN
cord-299281-5z1xminb	109	59	.	.	.
cord-299281-5z1xminb	110	1	For	for	IN
cord-299281-5z1xminb	110	2	estimating	estimate	VBG
cord-299281-5z1xminb	110	3	the	the	DT
cord-299281-5z1xminb	110	4	size	size	NN
cord-299281-5z1xminb	110	5	of	of	IN
cord-299281-5z1xminb	110	6	the	the	DT
cord-299281-5z1xminb	110	7	oligomer	oligomer	NNP
cord-299281-5z1xminb	110	8	,	,	,
cord-299281-5z1xminb	110	9	gradients	gradient	NNS
cord-299281-5z1xminb	110	10	(	(	-LRB-
cord-299281-5z1xminb	110	11	5	5	CD
cord-299281-5z1xminb	110	12	-	-	SYM
cord-299281-5z1xminb	110	13	20	20	CD
cord-299281-5z1xminb	110	14	%	%	NN
cord-299281-5z1xminb	110	15	sucrose	sucrose	NN
cord-299281-5z1xminb	110	16	[	[	-LRB-
cord-299281-5z1xminb	110	17	wt	wt	NNP
cord-299281-5z1xminb	110	18	/	/	SYM
cord-299281-5z1xminb	110	19	wt	wt	NNP
cord-299281-5z1xminb	110	20	]	]	-RRB-
cord-299281-5z1xminb	110	21	in	in	IN
cord-299281-5z1xminb	110	22	100	100	CD
cord-299281-5z1xminb	110	23	mM	mm	CD
cord-299281-5z1xminb	110	24	NaC1	NaC1	NNPS
cord-299281-5z1xminb	110	25	,	,	,
cord-299281-5z1xminb	110	26	50	50	CD
cord-299281-5z1xminb	110	27	mM	mm	CD
cord-299281-5z1xminb	110	28	Tris	Tris	NNP
cord-299281-5z1xminb	110	29	,	,	,
cord-299281-5z1xminb	110	30	pH	pH	NNP
cord-299281-5z1xminb	110	31	5.8	5.8	CD
cord-299281-5z1xminb	110	32	,	,	,
cord-299281-5z1xminb	110	33	0.1	0.1	CD
cord-299281-5z1xminb	110	34	%	%	NN
cord-299281-5z1xminb	110	35	Triton	Triton	NNP
cord-299281-5z1xminb	110	36	X-100	x-100	NN
cord-299281-5z1xminb	110	37	)	)	-RRB-
cord-299281-5z1xminb	110	38	were	be	VBD
cord-299281-5z1xminb	110	39	spun	spin	VBN
cord-299281-5z1xminb	110	40	at	at	IN
cord-299281-5z1xminb	110	41	47000	47000	CD
cord-299281-5z1xminb	110	42	rpm	rpm	NN
cord-299281-5z1xminb	110	43	for	for	IN
cord-299281-5z1xminb	110	44	4	4	CD
cord-299281-5z1xminb	110	45	h.	h.	NN
cord-299281-5z1xminb	111	1	The	the	DT
cord-299281-5z1xminb	111	2	markers	marker	NNS
cord-299281-5z1xminb	111	3	used	use	VBN
cord-299281-5z1xminb	111	4	were	be	VBD
cord-299281-5z1xminb	111	5	thyroglobulin	thyroglobulin	NNP
cord-299281-5z1xminb	111	6	(	(	-LRB-
cord-299281-5z1xminb	111	7	19.3	19.3	CD
cord-299281-5z1xminb	111	8	$	$	$
cord-299281-5z1xminb	111	9	20,,0	20,,0	CD
cord-299281-5z1xminb	111	10	)	)	-RRB-
cord-299281-5z1xminb	111	11	and	and	CC
cord-299281-5z1xminb	111	12	eatalase	eatalase	NNP
cord-299281-5z1xminb	111	13	(	(	-LRB-
cord-299281-5z1xminb	111	14	11.3	11.3	CD
cord-299281-5z1xminb	111	15	$	$	$
cord-299281-5z1xminb	111	16	20,,0	20,,0	CD
cord-299281-5z1xminb	111	17	)	)	-RRB-
cord-299281-5z1xminb	111	18	.	.	.
cord-299281-5z1xminb	112	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	112	2	were	be	VBD
cord-299281-5z1xminb	112	3	prepared	prepare	VBN
cord-299281-5z1xminb	112	4	after	after	IN
cord-299281-5z1xminb	112	5	metabolic	metabolic	JJ
cord-299281-5z1xminb	112	6	labeling	labeling	NN
cord-299281-5z1xminb	112	7	and	and	CC
cord-299281-5z1xminb	112	8	the	the	DT
cord-299281-5z1xminb	112	9	appropriate	appropriate	JJ
cord-299281-5z1xminb	112	10	chase	chase	NN
cord-299281-5z1xminb	112	11	period	period	NN
cord-299281-5z1xminb	112	12	from	from	IN
cord-299281-5z1xminb	112	13	transfected	transfecte	VBN
cord-299281-5z1xminb	112	14	HeLa	HeLa	NNP
cord-299281-5z1xminb	112	15	cells	cell	NNS
cord-299281-5z1xminb	112	16	plated	plate	VBN
cord-299281-5z1xminb	112	17	in	in	IN
cord-299281-5z1xminb	112	18	6-cm	6-cm	CD
cord-299281-5z1xminb	112	19	dishes	dish	NNS
cord-299281-5z1xminb	112	20	.	.	.
cord-299281-5z1xminb	113	1	Cells	cell	NNS
cord-299281-5z1xminb	113	2	were	be	VBD
cord-299281-5z1xminb	113	3	rinsed	rinse	VBN
cord-299281-5z1xminb	113	4	in	in	IN
cord-299281-5z1xminb	113	5	PBS	PBS	NNP
cord-299281-5z1xminb	113	6	and	and	CC
cord-299281-5z1xminb	113	7	swelled	swell	VBD
cord-299281-5z1xminb	113	8	in	in	IN
cord-299281-5z1xminb	113	9	0.8	0.8	CD
cord-299281-5z1xminb	113	10	ml	ml	NN
cord-299281-5z1xminb	113	11	swelling	swell	VBG
cord-299281-5z1xminb	113	12	buffer	buffer	NN
cord-299281-5z1xminb	113	13	(	(	-LRB-
cord-299281-5z1xminb	113	14	10	10	CD
cord-299281-5z1xminb	113	15	mM	mM	NNP
cord-299281-5z1xminb	113	16	Tris	Tris	NNP
cord-299281-5z1xminb	113	17	,	,	,
cord-299281-5z1xminb	113	18	15	15	CD
cord-299281-5z1xminb	113	19	mM	mm	CD
cord-299281-5z1xminb	113	20	NaCi	NaCi	NNPS
cord-299281-5z1xminb	113	21	,	,	,
cord-299281-5z1xminb	113	22	t	t	NN
cord-299281-5z1xminb	113	23	mM	mM	NNP
cord-299281-5z1xminb	113	24	MgCI2	MgCI2	NNP
cord-299281-5z1xminb	113	25	,	,	,
cord-299281-5z1xminb	113	26	pH	pH	NNP
cord-299281-5z1xminb	113	27	7.4	7.4	CD
cord-299281-5z1xminb	113	28	)	)	-RRB-
cord-299281-5z1xminb	113	29	for	for	IN
cord-299281-5z1xminb	113	30	5	5	CD
cord-299281-5z1xminb	113	31	rain	rain	NN
cord-299281-5z1xminb	113	32	on	on	IN
cord-299281-5z1xminb	113	33	ice	ice	NN
cord-299281-5z1xminb	113	34	.	.	.
cord-299281-5z1xminb	114	1	After	after	IN
cord-299281-5z1xminb	114	2	scraping	scrape	VBG
cord-299281-5z1xminb	114	3	with	with	IN
cord-299281-5z1xminb	114	4	a	a	DT
cord-299281-5z1xminb	114	5	rubbet	rubbet	NN
cord-299281-5z1xminb	114	6	policeman	policeman	NN
cord-299281-5z1xminb	114	7	,	,	,
cord-299281-5z1xminb	114	8	0.25	0.25	CD
cord-299281-5z1xminb	114	9	ml	ml	NN
cord-299281-5z1xminb	114	10	swelling	swell	VBG
cord-299281-5z1xminb	114	11	buffer	buffer	NN
cord-299281-5z1xminb	114	12	containing	contain	VBG
cord-299281-5z1xminb	114	13	40	40	CD
cord-299281-5z1xminb	114	14	%	%	NN
cord-299281-5z1xminb	114	15	sucrose	sucrose	NN
cord-299281-5z1xminb	114	16	(	(	-LRB-
cord-299281-5z1xminb	114	17	wt	wt	NN
cord-299281-5z1xminb	114	18	/	/	SYM
cord-299281-5z1xminb	114	19	vol	vol	NN
cord-299281-5z1xminb	114	20	)	)	-RRB-
cord-299281-5z1xminb	114	21	was	be	VBD
cord-299281-5z1xminb	114	22	added	add	VBN
cord-299281-5z1xminb	114	23	,	,	,
cord-299281-5z1xminb	114	24	and	and	CC
cord-299281-5z1xminb	114	25	the	the	DT
cord-299281-5z1xminb	114	26	cells	cell	NNS
cord-299281-5z1xminb	114	27	were	be	VBD
cord-299281-5z1xminb	114	28	homogenized	homogenize	VBN
cord-299281-5z1xminb	114	29	in	in	IN
cord-299281-5z1xminb	114	30	a	a	DT
cord-299281-5z1xminb	114	31	I	i	NN
cord-299281-5z1xminb	114	32	ml	ml	NN
cord-299281-5z1xminb	114	33	Dounce	Dounce	NNP
cord-299281-5z1xminb	114	34	homogenizer	homogenizer	NN
cord-299281-5z1xminb	114	35	with	with	IN
cord-299281-5z1xminb	114	36	60	60	CD
cord-299281-5z1xminb	114	37	strokes	stroke	NNS
cord-299281-5z1xminb	114	38	of	of	IN
cord-299281-5z1xminb	114	39	the	the	DT
cord-299281-5z1xminb	114	40	A	A	NNP
cord-299281-5z1xminb	114	41	pestle	pestle	NN
cord-299281-5z1xminb	114	42	.	.	.
cord-299281-5z1xminb	115	1	Lysates	Lysates	NNP
cord-299281-5z1xminb	115	2	were	be	VBD
cord-299281-5z1xminb	115	3	centrifuged	centrifuge	VBN
cord-299281-5z1xminb	115	4	briefly	briefly	RB
cord-299281-5z1xminb	115	5	(	(	-LRB-
cord-299281-5z1xminb	115	6	90	90	CD
cord-299281-5z1xminb	115	7	s	s	NN
cord-299281-5z1xminb	115	8	5,000	5,000	CD
cord-299281-5z1xminb	115	9	rpm	rpm	NN
cord-299281-5z1xminb	115	10	)	)	-RRB-
cord-299281-5z1xminb	115	11	to	to	TO
cord-299281-5z1xminb	115	12	remove	remove	VB
cord-299281-5z1xminb	115	13	nuclei	nucleus	NNS
cord-299281-5z1xminb	115	14	and	and	CC
cord-299281-5z1xminb	115	15	large	large	JJ
cord-299281-5z1xminb	115	16	debris	debris	NN
cord-299281-5z1xminb	115	17	.	.	.
cord-299281-5z1xminb	116	1	0.3	0.3	CD
cord-299281-5z1xminb	116	2	ml	ml	NN
cord-299281-5z1xminb	116	3	swelling	swell	VBG
cord-299281-5z1xminb	116	4	buffer	buffer	NN
cord-299281-5z1xminb	116	5	containing	contain	VBG
cord-299281-5z1xminb	116	6	10	10	CD
cord-299281-5z1xminb	116	7	%	%	NN
cord-299281-5z1xminb	116	8	sucrose	sucrose	NN
cord-299281-5z1xminb	116	9	(	(	-LRB-
cord-299281-5z1xminb	116	10	wt	wt	NN
cord-299281-5z1xminb	116	11	/	/	SYM
cord-299281-5z1xminb	116	12	vol	vol	NN
cord-299281-5z1xminb	116	13	)	)	-RRB-
cord-299281-5z1xminb	116	14	was	be	VBD
cord-299281-5z1xminb	116	15	added	add	VBN
cord-299281-5z1xminb	116	16	,	,	,
cord-299281-5z1xminb	116	17	and	and	CC
cord-299281-5z1xminb	116	18	the	the	DT
cord-299281-5z1xminb	116	19	lysates	lysate	NNS
cord-299281-5z1xminb	116	20	were	be	VBD
cord-299281-5z1xminb	116	21	centrifuged	centrifuge	VBN
cord-299281-5z1xminb	116	22	for	for	IN
cord-299281-5z1xminb	116	23	10	10	CD
cord-299281-5z1xminb	116	24	min	min	NN
cord-299281-5z1xminb	116	25	at	at	IN
cord-299281-5z1xminb	116	26	100,000	100,000	CD
cord-299281-5z1xminb	116	27	rpm	rpm	NN
cord-299281-5z1xminb	116	28	in	in	IN
cord-299281-5z1xminb	116	29	a	a	DT
cord-299281-5z1xminb	116	30	Beckman	Beckman	NNP
cord-299281-5z1xminb	116	31	TL-100	TL-100	NNP
cord-299281-5z1xminb	116	32	ultracentrifuge	ultracentrifuge	JJ
cord-299281-5z1xminb	116	33	(	(	-LRB-
cord-299281-5z1xminb	116	34	T100.2	T100.2	NNP
cord-299281-5z1xminb	116	35	rotor	rotor	NN
cord-299281-5z1xminb	116	36	)	)	-RRB-
cord-299281-5z1xminb	116	37	.	.	.
cord-299281-5z1xminb	117	1	Pellets	pellet	NNS
cord-299281-5z1xminb	117	2	were	be	VBD
cord-299281-5z1xminb	117	3	resuspended	resuspend	VBN
cord-299281-5z1xminb	117	4	gently	gently	RB
cord-299281-5z1xminb	117	5	in	in	IN
cord-299281-5z1xminb	117	6	PBS	PBS	NNP
cord-299281-5z1xminb	117	7	and	and	CC
cord-299281-5z1xminb	117	8	divided	divide	VBD
cord-299281-5z1xminb	117	9	into	into	IN
cord-299281-5z1xminb	117	10	two	two	CD
cord-299281-5z1xminb	117	11	aiiquots	aiiquot	NNS
cord-299281-5z1xminb	117	12	.	.	.
cord-299281-5z1xminb	118	1	TPCK	TPCK	NNP
cord-299281-5z1xminb	118	2	-	-	HYPH
cord-299281-5z1xminb	118	3	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	118	4	(	(	-LRB-
cord-299281-5z1xminb	118	5	20/~g	20/~g	NNP
cord-299281-5z1xminb	118	6	)	)	-RRB-
cord-299281-5z1xminb	118	7	was	be	VBD
cord-299281-5z1xminb	118	8	added	add	VBN
cord-299281-5z1xminb	118	9	to	to	IN
cord-299281-5z1xminb	118	10	one	one	CD
cord-299281-5z1xminb	118	11	aliquot	aliquot	NN
cord-299281-5z1xminb	118	12	,	,	,
cord-299281-5z1xminb	118	13	and	and	CC
cord-299281-5z1xminb	118	14	both	both	DT
cord-299281-5z1xminb	118	15	were	be	VBD
cord-299281-5z1xminb	118	16	incubated	incubate	VBN
cord-299281-5z1xminb	118	17	at	at	IN
cord-299281-5z1xminb	118	18	37"C	37"c	CD
cord-299281-5z1xminb	118	19	for	for	IN
cord-299281-5z1xminb	118	20	30	30	CD
cord-299281-5z1xminb	118	21	min	min	NN
cord-299281-5z1xminb	118	22	.	.	.
cord-299281-5z1xminb	119	1	Proteolytic	proteolytic	JJ
cord-299281-5z1xminb	119	2	digestion	digestion	NN
cord-299281-5z1xminb	119	3	was	be	VBD
cord-299281-5z1xminb	119	4	stopped	stop	VBN
cord-299281-5z1xminb	119	5	by	by	IN
cord-299281-5z1xminb	119	6	adding	add	VBG
cord-299281-5z1xminb	119	7	20	20	CD
cord-299281-5z1xminb	119	8	#	#	$
cord-299281-5z1xminb	119	9	g	g	NN
cord-299281-5z1xminb	119	10	of	of	IN
cord-299281-5z1xminb	119	11	soybean	soybean	NN
cord-299281-5z1xminb	119	12	trypsin	trypsin	NN
cord-299281-5z1xminb	119	13	inhibitor	inhibitor	NN
cord-299281-5z1xminb	119	14	to	to	IN
cord-299281-5z1xminb	119	15	both	both	DT
cord-299281-5z1xminb	119	16	tubes	tube	NNS
cord-299281-5z1xminb	119	17	.	.	.
cord-299281-5z1xminb	120	1	Samples	sample	NNS
cord-299281-5z1xminb	120	2	were	be	VBD
cord-299281-5z1xminb	120	3	solubilized	solubilize	VBN
cord-299281-5z1xminb	120	4	(	(	-LRB-
cord-299281-5z1xminb	120	5	in	in	IN
cord-299281-5z1xminb	120	6	detergent	detergent	NN
cord-299281-5z1xminb	120	7	solution	solution	NN
cord-299281-5z1xminb	120	8	containing	contain	VBG
cord-299281-5z1xminb	120	9	0.4	0.4	CD
cord-299281-5z1xminb	120	10	%	%	NN
cord-299281-5z1xminb	120	11	SDS	SDS	NNP
cord-299281-5z1xminb	120	12	or	or	CC
cord-299281-5z1xminb	120	13	in	in	IN
cord-299281-5z1xminb	120	14	MNT	MNT	NNP
cord-299281-5z1xminb	120	15	as	as	IN
cord-299281-5z1xminb	120	16	noted	note	VBN
cord-299281-5z1xminb	120	17	)	)	-RRB-
cord-299281-5z1xminb	120	18	and	and	CC
cord-299281-5z1xminb	120	19	processed	process	VBN
cord-299281-5z1xminb	120	20	further	further	RB
cord-299281-5z1xminb	120	21	as	as	IN
cord-299281-5z1xminb	120	22	described	describe	VBN
cord-299281-5z1xminb	120	23	in	in	IN
cord-299281-5z1xminb	120	24	the	the	DT
cord-299281-5z1xminb	120	25	text	text	NN
cord-299281-5z1xminb	120	26	and	and	CC
cord-299281-5z1xminb	120	27	figure	figure	NN
cord-299281-5z1xminb	120	28	legends	legend	NNS
cord-299281-5z1xminb	120	29	.	.	.
cord-299281-5z1xminb	121	1	Previous	previous	JJ
cord-299281-5z1xminb	121	2	studies	study	NNS
cord-299281-5z1xminb	121	3	from	from	IN
cord-299281-5z1xminb	121	4	this	this	DT
cord-299281-5z1xminb	121	5	laboratory	laboratory	NN
cord-299281-5z1xminb	121	6	showed	show	VBD
cord-299281-5z1xminb	121	7	that	that	IN
cord-299281-5z1xminb	121	8	the	the	DT
cord-299281-5z1xminb	121	9	first	first	JJ
cord-299281-5z1xminb	121	10	membrane	membrane	NN
cord-299281-5z1xminb	121	11	span	span	NN
cord-299281-5z1xminb	121	12	(	(	-LRB-
cord-299281-5z1xminb	121	13	ml	ml	NN
cord-299281-5z1xminb	121	14	)	)	-RRB-
cord-299281-5z1xminb	121	15	of	of	IN
cord-299281-5z1xminb	121	16	the	the	DT
cord-299281-5z1xminb	121	17	M	M	NNP
cord-299281-5z1xminb	121	18	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	121	19	of	of	IN
cord-299281-5z1xminb	121	20	1BV	1bv	CD
cord-299281-5z1xminb	121	21	(	(	-LRB-
cord-299281-5z1xminb	121	22	previously	previously	RB
cord-299281-5z1xminb	121	23	called	call	VBN
cord-299281-5z1xminb	121	24	the	the	DT
cord-299281-5z1xminb	121	25	E1	e1	NN
cord-299281-5z1xminb	121	26	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	121	27	)	)	-RRB-
cord-299281-5z1xminb	121	28	causes	cause	VBZ
cord-299281-5z1xminb	121	29	the	the	DT
cord-299281-5z1xminb	121	30	chimeric	chimeric	JJ
cord-299281-5z1xminb	121	31	protein	protein	NN
cord-299281-5z1xminb	121	32	Gml	Gml	NNP
cord-299281-5z1xminb	121	33	to	to	TO
cord-299281-5z1xminb	121	34	be	be	VB
cord-299281-5z1xminb	121	35	retained	retain	VBN
cord-299281-5z1xminb	121	36	in	in	IN
cord-299281-5z1xminb	121	37	the	the	DT
cord-299281-5z1xminb	121	38	Golgi	Golgi	NNP
cord-299281-5z1xminb	121	39	.	.	.
cord-299281-5z1xminb	122	1	The	the	DT
cord-299281-5z1xminb	122	2	sequence	sequence	NN
cord-299281-5z1xminb	122	3	of	of	IN
cord-299281-5z1xminb	122	4	ml	ml	NN
cord-299281-5z1xminb	122	5	is	be	VBZ
cord-299281-5z1xminb	122	6	shown	show	VBN
cord-299281-5z1xminb	122	7	in	in	IN
cord-299281-5z1xminb	122	8	Fig	Fig	NNP
cord-299281-5z1xminb	122	9	.	.	.
cord-299281-5z1xminb	123	1	1	1	CD
cord-299281-5z1xminb	123	2	A.	a.	NN
cord-299281-5z1xminb	123	3	Using	use	VBG
cord-299281-5z1xminb	123	4	site	site	NN
cord-299281-5z1xminb	123	5	directed	direct	VBN
cord-299281-5z1xminb	123	6	mutagenesis	mutagenesis	NN
cord-299281-5z1xminb	124	1	,	,	,
cord-299281-5z1xminb	124	2	four	four	CD
cord-299281-5z1xminb	124	3	key	key	JJ
cord-299281-5z1xminb	124	4	residues	residue	NNS
cord-299281-5z1xminb	124	5	in	in	IN
cord-299281-5z1xminb	124	6	the	the	DT
cord-299281-5z1xminb	124	7	ml	ml	NN
cord-299281-5z1xminb	124	8	domain	domain	NN
cord-299281-5z1xminb	124	9	were	be	VBD
cord-299281-5z1xminb	124	10	shown	show	VBN
cord-299281-5z1xminb	124	11	to	to	TO
cord-299281-5z1xminb	124	12	be	be	VB
cord-299281-5z1xminb	124	13	critical	critical	JJ
cord-299281-5z1xminb	124	14	for	for	IN
cord-299281-5z1xminb	124	15	Golgi	Golgi	NNP
cord-299281-5z1xminb	124	16	retention	retention	NN
cord-299281-5z1xminb	124	17	of	of	IN
cord-299281-5z1xminb	124	18	Gml	Gml	NNP
cord-299281-5z1xminb	124	19	:	:	:
cord-299281-5z1xminb	124	20	asparagine	asparagine	NN
cord-299281-5z1xminb	124	21	(	(	-LRB-
cord-299281-5z1xminb	124	22	N,65	N,65	NNP
cord-299281-5z1xminb	124	23	)	)	-RRB-
cord-299281-5z1xminb	124	24	,	,	,
cord-299281-5z1xminb	124	25	two	two	CD
cord-299281-5z1xminb	124	26	threonines	threonine	NNS
cord-299281-5z1xminb	124	27	(	(	-LRB-
cord-299281-5z1xminb	124	28	T~	T~	NNP
cord-299281-5z1xminb	124	29	and	and	CC
cord-299281-5z1xminb	124	30	T,76	T,76	NNP
cord-299281-5z1xminb	124	31	)	)	-RRB-
cord-299281-5z1xminb	124	32	,	,	,
cord-299281-5z1xminb	124	33	and	and	CC
cord-299281-5z1xminb	124	34	glutamine	glutamine	NN
cord-299281-5z1xminb	124	35	(	(	-LRB-
cord-299281-5z1xminb	124	36	Q~	Q~	NNP
cord-299281-5z1xminb	124	37	)	)	-RRB-
cord-299281-5z1xminb	124	38	.	.	.
cord-299281-5z1xminb	125	1	Some	some	DT
cord-299281-5z1xminb	125	2	substitutions	substitution	NNS
cord-299281-5z1xminb	125	3	at	at	IN
cord-299281-5z1xminb	125	4	Q~0	Q~0	NNS
cord-299281-5z1xminb	125	5	are	be	VBP
cord-299281-5z1xminb	125	6	tolerated	tolerate	VBN
cord-299281-5z1xminb	125	7	(	(	-LRB-
cord-299281-5z1xminb	125	8	e.g.	e.g.	RB
cord-299281-5z1xminb	125	9	,	,	,
cord-299281-5z1xminb	125	10	GmlQI'hs0	gmlqi'hs0	CD
cord-299281-5z1xminb	125	11	)	)	-RRB-
cord-299281-5z1xminb	126	1	whereas	whereas	IN
cord-299281-5z1xminb	126	2	other	other	JJ
cord-299281-5z1xminb	126	3	changes	change	NNS
cord-299281-5z1xminb	127	1	(	(	-LRB-
cord-299281-5z1xminb	127	2	e.g.	e.g.	RB
cord-299281-5z1xminb	127	3	,	,	,
cord-299281-5z1xminb	127	4	GmlQI	gmlqi	NN
cord-299281-5z1xminb	127	5	~	~	SYM
cord-299281-5z1xminb	127	6	o	o	NN
cord-299281-5z1xminb	127	7	)	)	-RRB-
cord-299281-5z1xminb	127	8	result	result	VB
cord-299281-5z1xminb	127	9	in	in	IN
cord-299281-5z1xminb	127	10	transport	transport	NN
cord-299281-5z1xminb	127	11	of	of	IN
cord-299281-5z1xminb	127	12	the	the	DT
cord-299281-5z1xminb	127	13	protein	protein	NN
cord-299281-5z1xminb	127	14	to	to	IN
cord-299281-5z1xminb	127	15	the	the	DT
cord-299281-5z1xminb	127	16	cell	cell	NN
cord-299281-5z1xminb	127	17	surface	surface	NN
cord-299281-5z1xminb	127	18	(	(	-LRB-
cord-299281-5z1xminb	127	19	Machamer	Machamer	NNP
cord-299281-5z1xminb	127	20	et	et	NNP
cord-299281-5z1xminb	127	21	al	al	NNP
cord-299281-5z1xminb	127	22	.	.	NNP
cord-299281-5z1xminb	127	23	,	,	,
cord-299281-5z1xminb	127	24	1993	1993	CD
cord-299281-5z1xminb	127	25	)	)	-RRB-
cord-299281-5z1xminb	127	26	.	.	.
cord-299281-5z1xminb	128	1	Another	another	DT
cord-299281-5z1xminb	128	2	mutation	mutation	NN
cord-299281-5z1xminb	128	3	in	in	IN
cord-299281-5z1xminb	128	4	which	which	WDT
cord-299281-5z1xminb	128	5	two	two	CD
cord-299281-5z1xminb	128	6	isoleucine	isoleucine	JJ
cord-299281-5z1xminb	128	7	residues	residue	NNS
cord-299281-5z1xminb	128	8	were	be	VBD
cord-299281-5z1xminb	128	9	inserted	insert	VBN
cord-299281-5z1xminb	128	10	near	near	IN
cord-299281-5z1xminb	128	11	the	the	DT
cord-299281-5z1xminb	128	12	center	center	NN
cord-299281-5z1xminb	128	13	of	of	IN
cord-299281-5z1xminb	128	14	the	the	DT
cord-299281-5z1xminb	128	15	transmembrane	transmembrane	NNP
cord-299281-5z1xminb	128	16	domain	domain	NN
cord-299281-5z1xminb	128	17	(	(	-LRB-
cord-299281-5z1xminb	128	18	Gmli,~	Gmli,~	NNP
cord-299281-5z1xminb	128	19	)	)	-RRB-
cord-299281-5z1xminb	128	20	also	also	RB
cord-299281-5z1xminb	128	21	disrupts	disrupt	VBZ
cord-299281-5z1xminb	128	22	retention	retention	NN
cord-299281-5z1xminb	128	23	in	in	IN
cord-299281-5z1xminb	128	24	the	the	DT
cord-299281-5z1xminb	128	25	Golgi	Golgi	NNP
cord-299281-5z1xminb	128	26	complex	complex	NN
cord-299281-5z1xminb	128	27	.	.	.
cord-299281-5z1xminb	129	1	These	these	DT
cord-299281-5z1xminb	129	2	three	three	CD
cord-299281-5z1xminb	129	3	chimeric	chimeric	JJ
cord-299281-5z1xminb	129	4	proteins	protein	NNS
cord-299281-5z1xminb	129	5	(	(	-LRB-
cord-299281-5z1xminb	129	6	GmL~	gml~	JJ
cord-299281-5z1xminb	129	7	,	,	,
cord-299281-5z1xminb	129	8	GmlQLso	GmlQLso	NNP
cord-299281-5z1xminb	129	9	,	,	,
cord-299281-5z1xminb	129	10	and	and	CC
cord-299281-5z1xminb	129	11	GmlQH_~o	GmlQH_~o	NNP
cord-299281-5z1xminb	129	12	)	)	-RRB-
cord-299281-5z1xminb	129	13	were	be	VBD
cord-299281-5z1xminb	129	14	expressed	express	VBN
cord-299281-5z1xminb	129	15	in	in	IN
cord-299281-5z1xminb	129	16	transiently	transiently	RB
cord-299281-5z1xminb	129	17	transfected	transfecte	VBN
cord-299281-5z1xminb	129	18	COS-'/	COS-'/	NNP
cord-299281-5z1xminb	129	19	cells	cell	NNS
cord-299281-5z1xminb	129	20	and	and	CC
cord-299281-5z1xminb	129	21	localized	localize	VBN
cord-299281-5z1xminb	129	22	by	by	IN
cord-299281-5z1xminb	129	23	indirect	indirect	JJ
cord-299281-5z1xminb	129	24	immunofluorescence	immunofluorescence	NN
cord-299281-5z1xminb	129	25	(	(	-LRB-
cord-299281-5z1xminb	129	26	Fig	fig	NN
cord-299281-5z1xminb	129	27	.	.	.
cord-299281-5z1xminb	129	28	1	1	CD
cord-299281-5z1xminb	129	29	B	b	NN
cord-299281-5z1xminb	129	30	)	)	-RRB-
cord-299281-5z1xminb	129	31	.	.	.
cord-299281-5z1xminb	130	1	VSV	VSV	NNP
cord-299281-5z1xminb	130	2	G	G	NNP
cord-299281-5z1xminb	130	3	and	and	CC
cord-299281-5z1xminb	130	4	GrnlQI	GrnlQI	NNP
cord-299281-5z1xminb	131	1	~o	~o	NNP
cord-299281-5z1xminb	131	2	were	be	VBD
cord-299281-5z1xminb	131	3	readily	readily	RB
cord-299281-5z1xminb	131	4	detected	detect	VBN
cord-299281-5z1xminb	131	5	at	at	IN
cord-299281-5z1xminb	131	6	the	the	DT
cord-299281-5z1xminb	131	7	plasma	plasma	NN
cord-299281-5z1xminb	131	8	membrane	membrane	NN
cord-299281-5z1xminb	131	9	,	,	,
cord-299281-5z1xminb	131	10	while	while	IN
cord-299281-5z1xminb	131	11	Gml	Gml	NNP
cord-299281-5z1xminb	131	12	and	and	CC
cord-299281-5z1xminb	131	13	GmlQI	GmlQI	NNP
cord-299281-5z1xminb	131	14	-	-	HYPH
cord-299281-5z1xminb	131	15	hso	hso	NNP
cord-299281-5z1xminb	131	16	were	be	VBD
cord-299281-5z1xminb	131	17	detected	detect	VBN
cord-299281-5z1xminb	131	18	only	only	RB
cord-299281-5z1xminb	131	19	in	in	IN
cord-299281-5z1xminb	131	20	the	the	DT
cord-299281-5z1xminb	131	21	Golgi	Golgi	NNP
cord-299281-5z1xminb	131	22	region	region	NN
cord-299281-5z1xminb	131	23	and	and	CC
cord-299281-5z1xminb	131	24	largely	largely	RB
cord-299281-5z1xminb	131	25	colocalized	colocalize	VBN
cord-299281-5z1xminb	131	26	with	with	IN
cord-299281-5z1xminb	131	27	lens	lens	NNP
cord-299281-5z1xminb	131	28	culinaris	culinaris	NN
cord-299281-5z1xminb	131	29	lectin	lectin	NN
cord-299281-5z1xminb	131	30	staining	stain	VBG
cord-299281-5z1xminb	131	31	,	,	,
cord-299281-5z1xminb	131	32	even	even	RB
cord-299281-5z1xminb	131	33	after	after	IN
cord-299281-5z1xminb	131	34	treatment	treatment	NN
cord-299281-5z1xminb	131	35	with	with	IN
cord-299281-5z1xminb	131	36	nocodazole	nocodazole	NNP
cord-299281-5z1xminb	131	37	or	or	CC
cord-299281-5z1xminb	131	38	brefeldin	brefeldin	NNP
cord-299281-5z1xminb	131	39	A	A	NNP
cord-299281-5z1xminb	132	1	(	(	-LRB-
cord-299281-5z1xminb	132	2	Machamer	Machamer	NNP
cord-299281-5z1xminb	132	3	et	et	NNP
cord-299281-5z1xminb	132	4	al	al	NNP
cord-299281-5z1xminb	132	5	.	.	NNP
cord-299281-5z1xminb	132	6	,	,	,
cord-299281-5z1xminb	132	7	1993	1993	CD
cord-299281-5z1xminb	132	8	)	)	-RRB-
cord-299281-5z1xminb	132	9	.	.	.
cord-299281-5z1xminb	133	1	Gmlin~	gmlin~	ADD
cord-299281-5z1xminb	134	1	staining	stain	VBG
cord-299281-5z1xminb	134	2	was	be	VBD
cord-299281-5z1xminb	134	3	less	less	RBR
cord-299281-5z1xminb	134	4	prominent	prominent	JJ
cord-299281-5z1xminb	134	5	at	at	IN
cord-299281-5z1xminb	134	6	the	the	DT
cord-299281-5z1xminb	134	7	plasma	plasma	NN
cord-299281-5z1xminb	134	8	membrane	membrane	NN
cord-299281-5z1xminb	134	9	than	than	IN
cord-299281-5z1xminb	134	10	GmlQI~0	GmlQI~0	NNP
cord-299281-5z1xminb	134	11	,	,	,
cord-299281-5z1xminb	134	12	and	and	CC
cord-299281-5z1xminb	134	13	a	a	DT
cord-299281-5z1xminb	134	14	considerable	considerable	JJ
cord-299281-5z1xminb	134	15	amount	amount	NN
cord-299281-5z1xminb	134	16	of	of	IN
cord-299281-5z1xminb	134	17	fluorescence	fluorescence	NN
cord-299281-5z1xminb	134	18	was	be	VBD
cord-299281-5z1xminb	134	19	detected	detect	VBN
cord-299281-5z1xminb	134	20	in	in	IN
cord-299281-5z1xminb	134	21	the	the	DT
cord-299281-5z1xminb	134	22	Golgi	Golgi	NNP
cord-299281-5z1xminb	134	23	complex	complex	NN
cord-299281-5z1xminb	134	24	.	.	.
cord-299281-5z1xminb	135	1	The	the	DT
cord-299281-5z1xminb	135	2	localizations	localization	NNS
cord-299281-5z1xminb	135	3	of	of	IN
cord-299281-5z1xminb	135	4	these	these	DT
cord-299281-5z1xminb	135	5	proteins	protein	NNS
cord-299281-5z1xminb	135	6	were	be	VBD
cord-299281-5z1xminb	135	7	confirmed	confirm	VBN
cord-299281-5z1xminb	135	8	in	in	IN
cord-299281-5z1xminb	135	9	HeLa	HeLa	NNP
cord-299281-5z1xminb	135	10	cells	cell	NNS
cord-299281-5z1xminb	135	11	and	and	CC
cord-299281-5z1xminb	135	12	BHK	BHK	NNP
cord-299281-5z1xminb	135	13	cells	cell	NNS
cord-299281-5z1xminb	135	14	transfected	transfecte	VBN
cord-299281-5z1xminb	135	15	using	use	VBG
cord-299281-5z1xminb	135	16	a	a	DT
cord-299281-5z1xminb	135	17	vaccinia	vaccinia	NN
cord-299281-5z1xminb	135	18	-	-	HYPH
cord-299281-5z1xminb	135	19	virusmediated	virusmediate	VBN
cord-299281-5z1xminb	135	20	expression	expression	NN
cord-299281-5z1xminb	135	21	system	system	NN
cord-299281-5z1xminb	135	22	(	(	-LRB-
cord-299281-5z1xminb	135	23	not	not	RB
cord-299281-5z1xminb	135	24	shown	show	VBN
cord-299281-5z1xminb	135	25	)	)	-RRB-
cord-299281-5z1xminb	135	26	.	.	.
cord-299281-5z1xminb	136	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	136	2	,	,	,
cord-299281-5z1xminb	136	3	when	when	WRB
cord-299281-5z1xminb	136	4	we	-PRON-	PRP
cord-299281-5z1xminb	136	5	analyzed	analyze	VBD
cord-299281-5z1xminb	136	6	expression	expression	NN
cord-299281-5z1xminb	136	7	of	of	IN
cord-299281-5z1xminb	136	8	these	these	DT
cord-299281-5z1xminb	136	9	constructs	construct	NNS
cord-299281-5z1xminb	136	10	in	in	IN
cord-299281-5z1xminb	136	11	HeLa	HeLa	NNP
cord-299281-5z1xminb	136	12	cells	cell	NNS
cord-299281-5z1xminb	136	13	and	and	CC
cord-299281-5z1xminb	136	14	COS-7	cos-7	NN
cord-299281-5z1xminb	136	15	cells	cell	NNS
cord-299281-5z1xminb	136	16	by	by	IN
cord-299281-5z1xminb	136	17	metabolic	metabolic	JJ
cord-299281-5z1xminb	136	18	radiolabeling	radiolabeling	NN
cord-299281-5z1xminb	136	19	and	and	CC
cord-299281-5z1xminb	136	20	immunoprecipitation	immunoprecipitation	NN
cord-299281-5z1xminb	136	21	,	,	,
cord-299281-5z1xminb	136	22	we	-PRON-	PRP
cord-299281-5z1xminb	136	23	noticed	notice	VBD
cord-299281-5z1xminb	136	24	the	the	DT
cord-299281-5z1xminb	136	25	appearance	appearance	NN
cord-299281-5z1xminb	136	26	of	of	IN
cord-299281-5z1xminb	136	27	a	a	DT
cord-299281-5z1xminb	136	28	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	136	29	SDS	sds	NN
cord-299281-5z1xminb	136	30	-	-	HYPH
cord-299281-5z1xminb	136	31	resistant	resistant	JJ
cord-299281-5z1xminb	136	32	species	specie	NNS
cord-299281-5z1xminb	136	33	that	that	WDT
cord-299281-5z1xminb	136	34	migrated	migrate	VBD
cord-299281-5z1xminb	136	35	at	at	IN
cord-299281-5z1xminb	136	36	the	the	DT
cord-299281-5z1xminb	136	37	top	top	NN
cord-299281-5z1xminb	136	38	of	of	IN
cord-299281-5z1xminb	136	39	the	the	DT
cord-299281-5z1xminb	136	40	stacking	stacking	NN
cord-299281-5z1xminb	136	41	gel	gel	NN
cord-299281-5z1xminb	136	42	,	,	,
cord-299281-5z1xminb	136	43	in	in	IN
cord-299281-5z1xminb	136	44	addition	addition	NN
cord-299281-5z1xminb	136	45	to	to	IN
cord-299281-5z1xminb	136	46	products	product	NNS
cord-299281-5z1xminb	136	47	migrating	migrate	VBG
cord-299281-5z1xminb	136	48	at	at	IN
cord-299281-5z1xminb	136	49	the	the	DT
cord-299281-5z1xminb	136	50	expected	expect	VBN
cord-299281-5z1xminb	136	51	molecular	molecular	JJ
cord-299281-5z1xminb	136	52	masses	masse	NNS
cord-299281-5z1xminb	136	53	.	.	.
cord-299281-5z1xminb	137	1	The	the	DT
cord-299281-5z1xminb	137	2	material	material	NN
cord-299281-5z1xminb	137	3	at	at	IN
cord-299281-5z1xminb	137	4	the	the	DT
cord-299281-5z1xminb	137	5	top	top	NN
cord-299281-5z1xminb	137	6	of	of	IN
cord-299281-5z1xminb	137	7	the	the	DT
cord-299281-5z1xminb	137	8	stacking	stacking	NN
cord-299281-5z1xminb	137	9	gel	gel	NN
cord-299281-5z1xminb	137	10	was	be	VBD
cord-299281-5z1xminb	137	11	more	more	RBR
cord-299281-5z1xminb	137	12	prevalent	prevalent	JJ
cord-299281-5z1xminb	137	13	in	in	IN
cord-299281-5z1xminb	137	14	samples	sample	NNS
cord-299281-5z1xminb	137	15	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	137	16	from	from	IN
cord-299281-5z1xminb	137	17	HeLa	HeLa	NNP
cord-299281-5z1xminb	137	18	cells	cell	NNS
cord-299281-5z1xminb	137	19	(	(	-LRB-
cord-299281-5z1xminb	137	20	Fig	fig	NN
cord-299281-5z1xminb	137	21	.	.	.
cord-299281-5z1xminb	137	22	1	1	CD
cord-299281-5z1xminb	137	23	C	C	NNP
cord-299281-5z1xminb	137	24	)	)	-RRB-
cord-299281-5z1xminb	137	25	than	than	IN
cord-299281-5z1xminb	137	26	in	in	IN
cord-299281-5z1xminb	137	27	those	those	DT
cord-299281-5z1xminb	137	28	from	from	IN
cord-299281-5z1xminb	137	29	COS-7	cos-7	JJ
cord-299281-5z1xminb	137	30	cells	cell	NNS
cord-299281-5z1xminb	137	31	(	(	-LRB-
cord-299281-5z1xminb	137	32	Fig	fig	NN
cord-299281-5z1xminb	137	33	.	.	.
cord-299281-5z1xminb	137	34	1	1	CD
cord-299281-5z1xminb	137	35	D	d	NN
cord-299281-5z1xminb	137	36	)	)	-RRB-
cord-299281-5z1xminb	137	37	.	.	.
cord-299281-5z1xminb	138	1	This	this	DT
cord-299281-5z1xminb	138	2	species	species	NN
cord-299281-5z1xminb	138	3	was	be	VBD
cord-299281-5z1xminb	138	4	present	present	JJ
cord-299281-5z1xminb	138	5	only	only	RB
cord-299281-5z1xminb	138	6	in	in	IN
cord-299281-5z1xminb	138	7	immunoprecipitates	immunoprecipitate	NNS
cord-299281-5z1xminb	138	8	from	from	IN
cord-299281-5z1xminb	138	9	cells	cell	NNS
cord-299281-5z1xminb	138	10	that	that	WDT
cord-299281-5z1xminb	138	11	expressed	express	VBD
cord-299281-5z1xminb	138	12	mutants	mutant	NNS
cord-299281-5z1xminb	138	13	with	with	IN
cord-299281-5z1xminb	138	14	Golgi	golgi	NN
cord-299281-5z1xminb	138	15	-	-	HYPH
cord-299281-5z1xminb	138	16	resident	resident	NN
cord-299281-5z1xminb	138	17	populations	population	NNS
cord-299281-5z1xminb	138	18	(	(	-LRB-
cord-299281-5z1xminb	138	19	Gml	Gml	NNP
cord-299281-5z1xminb	138	20	,	,	,
cord-299281-5z1xminb	138	21	Gmli	Gmli	NNP
cord-299281-5z1xminb	138	22	,	,	,
cord-299281-5z1xminb	138	23	,	,	,
cord-299281-5z1xminb	138	24	and	and	CC
cord-299281-5z1xminb	138	25	GmlQH4s0	GmlQH4s0	NNP
cord-299281-5z1xminb	138	26	)	)	-RRB-
cord-299281-5z1xminb	138	27	and	and	CC
cord-299281-5z1xminb	138	28	it	-PRON-	PRP
cord-299281-5z1xminb	138	29	appeared	appear	VBD
cord-299281-5z1xminb	138	30	regardless	regardless	RB
cord-299281-5z1xminb	138	31	of	of	IN
cord-299281-5z1xminb	138	32	the	the	DT
cord-299281-5z1xminb	138	33	level	level	NN
cord-299281-5z1xminb	138	34	of	of	IN
cord-299281-5z1xminb	138	35	expression	expression	NN
cord-299281-5z1xminb	138	36	(	(	-LRB-
cord-299281-5z1xminb	138	37	over	over	IN
cord-299281-5z1xminb	138	38	a	a	DT
cord-299281-5z1xminb	138	39	>	>	XX
cord-299281-5z1xminb	138	40	50-fold	50-fold	CD
cord-299281-5z1xminb	138	41	range	range	NN
cord-299281-5z1xminb	138	42	)	)	-RRB-
cord-299281-5z1xminb	138	43	.	.	.
cord-299281-5z1xminb	139	1	Although	although	IN
cord-299281-5z1xminb	139	2	~50	~50	NFP
cord-299281-5z1xminb	139	3	%	%	NN
cord-299281-5z1xminb	139	4	of	of	IN
cord-299281-5z1xminb	139	5	the	the	DT
cord-299281-5z1xminb	139	6	total	total	JJ
cord-299281-5z1xminb	139	7	radioactivity	radioactivity	NN
cord-299281-5z1xminb	139	8	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	139	9	from	from	IN
cord-299281-5z1xminb	139	10	Gml	Gml	NNP
cord-299281-5z1xminb	139	11	-	-	HYPH
cord-299281-5z1xminb	139	12	expressing	express	VBG
cord-299281-5z1xminb	139	13	HeLa	HeLa	NNP
cord-299281-5z1xminb	139	14	cells	cell	NNS
cord-299281-5z1xminb	139	15	migrated	migrate	VBD
cord-299281-5z1xminb	139	16	at	at	IN
cord-299281-5z1xminb	139	17	the	the	DT
cord-299281-5z1xminb	139	18	top	top	NN
cord-299281-5z1xminb	139	19	of	of	IN
cord-299281-5z1xminb	139	20	the	the	DT
cord-299281-5z1xminb	139	21	stacking	stacking	NN
cord-299281-5z1xminb	139	22	gel	gel	NN
cord-299281-5z1xminb	139	23	,	,	,
cord-299281-5z1xminb	139	24	we	-PRON-	PRP
cord-299281-5z1xminb	139	25	have	have	VBP
cord-299281-5z1xminb	139	26	not	not	RB
cord-299281-5z1xminb	139	27	quantitated	quantitate	VBN
cord-299281-5z1xminb	139	28	this	this	DT
cord-299281-5z1xminb	139	29	information	information	NN
cord-299281-5z1xminb	139	30	routinely	routinely	RB
cord-299281-5z1xminb	139	31	.	.	.
cord-299281-5z1xminb	140	1	One	one	CD
cord-299281-5z1xminb	140	2	problem	problem	NN
cord-299281-5z1xminb	140	3	with	with	IN
cord-299281-5z1xminb	140	4	quantitation	quantitation	NN
cord-299281-5z1xminb	140	5	is	be	VBZ
cord-299281-5z1xminb	140	6	that	that	IN
cord-299281-5z1xminb	140	7	this	this	DT
cord-299281-5z1xminb	140	8	material	material	NN
cord-299281-5z1xminb	140	9	could	could	MD
cord-299281-5z1xminb	140	10	include	include	VB
cord-299281-5z1xminb	140	11	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	140	12	components	component	NNS
cord-299281-5z1xminb	140	13	other	other	JJ
cord-299281-5z1xminb	140	14	than	than	IN
cord-299281-5z1xminb	140	15	Gml	Gml	NNP
cord-299281-5z1xminb	140	16	;	;	:
cord-299281-5z1xminb	140	17	furthermore	furthermore	RB
cord-299281-5z1xminb	140	18	,	,	,
cord-299281-5z1xminb	140	19	some	some	DT
cord-299281-5z1xminb	140	20	of	of	IN
cord-299281-5z1xminb	140	21	the	the	DT
cord-299281-5z1xminb	140	22	oligomer	oligomer	NNP
cord-299281-5z1xminb	140	23	may	may	MD
cord-299281-5z1xminb	140	24	not	not	RB
cord-299281-5z1xminb	140	25	enter	enter	VB
cord-299281-5z1xminb	140	26	the	the	DT
cord-299281-5z1xminb	140	27	gel	gel	NN
cord-299281-5z1xminb	140	28	at	at	RB
cord-299281-5z1xminb	140	29	all	all	RB
cord-299281-5z1xminb	140	30	and	and	CC
cord-299281-5z1xminb	140	31	be	be	VB
cord-299281-5z1xminb	140	32	lost	lose	VBN
cord-299281-5z1xminb	140	33	during	during	IN
cord-299281-5z1xminb	140	34	subsequent	subsequent	JJ
cord-299281-5z1xminb	140	35	processing	processing	NN
cord-299281-5z1xminb	140	36	.	.	.
cord-299281-5z1xminb	141	1	Gmli	Gmli	NNP
cord-299281-5z1xminb	141	2	,	,	,
cord-299281-5z1xminb	141	3	,	,	,
cord-299281-5z1xminb	141	4	was	be	VBD
cord-299281-5z1xminb	141	5	previously	previously	RB
cord-299281-5z1xminb	141	6	reported	report	VBN
cord-299281-5z1xminb	141	7	to	to	TO
cord-299281-5z1xminb	141	8	move	move	VB
cord-299281-5z1xminb	141	9	through	through	IN
cord-299281-5z1xminb	141	10	the	the	DT
cord-299281-5z1xminb	141	11	Golgi	Golgi	NNP
cord-299281-5z1xminb	141	12	complex	complex	NN
cord-299281-5z1xminb	141	13	rapidly	rapidly	RB
cord-299281-5z1xminb	141	14	(	(	-LRB-
cord-299281-5z1xminb	141	15	with	with	IN
cord-299281-5z1xminb	141	16	a	a	DT
cord-299281-5z1xminb	141	17	half	half	JJ
cord-299281-5z1xminb	141	18	time	time	NN
cord-299281-5z1xminb	141	19	of	of	IN
cord-299281-5z1xminb	141	20	35	35	CD
cord-299281-5z1xminb	141	21	rain	rain	NN
cord-299281-5z1xminb	141	22	in	in	IN
cord-299281-5z1xminb	141	23	COS-7	cos-7	NN
cord-299281-5z1xminb	141	24	cells	cell	NNS
cord-299281-5z1xminb	141	25	;	;	:
cord-299281-5z1xminb	141	26	Swift	Swift	NNP
cord-299281-5z1xminb	141	27	and	and	CC
cord-299281-5z1xminb	141	28	Machamer	Machamer	NNP
cord-299281-5z1xminb	141	29	,	,	,
cord-299281-5z1xminb	141	30	1991	1991	CD
cord-299281-5z1xminb	141	31	)	)	-RRB-
cord-299281-5z1xminb	141	32	.	.	.
cord-299281-5z1xminb	142	1	However	however	RB
cord-299281-5z1xminb	142	2	,	,	,
cord-299281-5z1xminb	142	3	the	the	DT
cord-299281-5z1xminb	142	4	kinetics	kinetic	NNS
cord-299281-5z1xminb	142	5	of	of	IN
cord-299281-5z1xminb	142	6	transport	transport	NN
cord-299281-5z1xminb	142	7	reflect	reflect	VBP
cord-299281-5z1xminb	142	8	only	only	RB
cord-299281-5z1xminb	142	9	the	the	DT
cord-299281-5z1xminb	142	10	population	population	NN
cord-299281-5z1xminb	142	11	of	of	IN
cord-299281-5z1xminb	142	12	protein	protein	NN
cord-299281-5z1xminb	142	13	that	that	WDT
cord-299281-5z1xminb	142	14	enters	enter	VBZ
cord-299281-5z1xminb	142	15	the	the	DT
cord-299281-5z1xminb	142	16	separating	separate	VBG
cord-299281-5z1xminb	142	17	gel	gel	NN
cord-299281-5z1xminb	142	18	.	.	.
cord-299281-5z1xminb	143	1	The	the	DT
cord-299281-5z1xminb	143	2	SDS	SDS	NNP
cord-299281-5z1xminb	143	3	-	-	HYPH
cord-299281-5z1xminb	143	4	resistant	resistant	JJ
cord-299281-5z1xminb	143	5	protein	protein	NN
cord-299281-5z1xminb	143	6	may	may	MD
cord-299281-5z1xminb	143	7	therefore	therefore	RB
cord-299281-5z1xminb	143	8	represent	represent	VB
cord-299281-5z1xminb	143	9	a	a	DT
cord-299281-5z1xminb	143	10	stable	stable	JJ
cord-299281-5z1xminb	143	11	Golgi	Golgi	NNP
cord-299281-5z1xminb	143	12	-	-	HYPH
cord-299281-5z1xminb	143	13	resident	resident	JJ
cord-299281-5z1xminb	143	14	pool	pool	NN
cord-299281-5z1xminb	143	15	.	.	.
cord-299281-5z1xminb	144	1	The	the	DT
cord-299281-5z1xminb	144	2	SDS	SDS	NNP
cord-299281-5z1xminb	144	3	-	-	HYPH
cord-299281-5z1xminb	144	4	resistant	resistant	JJ
cord-299281-5z1xminb	144	5	oligomerization	oligomerization	NN
cord-299281-5z1xminb	144	6	did	do	VBD
cord-299281-5z1xminb	144	7	not	not	RB
cord-299281-5z1xminb	144	8	depend	depend	VB
cord-299281-5z1xminb	144	9	on	on	IN
cord-299281-5z1xminb	144	10	the	the	DT
cord-299281-5z1xminb	144	11	type	type	NN
cord-299281-5z1xminb	144	12	of	of	IN
cord-299281-5z1xminb	144	13	cells	cell	NNS
cord-299281-5z1xminb	144	14	used	use	VBN
cord-299281-5z1xminb	144	15	for	for	IN
cord-299281-5z1xminb	144	16	expression	expression	NN
cord-299281-5z1xminb	144	17	,	,	,
cord-299281-5z1xminb	144	18	since	since	IN
cord-299281-5z1xminb	144	19	the	the	DT
cord-299281-5z1xminb	144	20	same	same	JJ
cord-299281-5z1xminb	144	21	results	result	NNS
cord-299281-5z1xminb	144	22	were	be	VBD
cord-299281-5z1xminb	144	23	found	find	VBN
cord-299281-5z1xminb	144	24	using	use	VBG
cord-299281-5z1xminb	144	25	transiently	transiently	RB
cord-299281-5z1xminb	144	26	transfected	transfecte	VBN
cord-299281-5z1xminb	144	27	BHK	BHK	NNP
cord-299281-5z1xminb	144	28	cells	cell	NNS
cord-299281-5z1xminb	144	29	and	and	CC
cord-299281-5z1xminb	144	30	stably	stably	RB
cord-299281-5z1xminb	144	31	transfected	transfecte	VBN
cord-299281-5z1xminb	144	32	CHO	CHO	NNP
cord-299281-5z1xminb	144	33	cells	cell	NNS
cord-299281-5z1xminb	144	34	(	(	-LRB-
cord-299281-5z1xminb	144	35	not	not	RB
cord-299281-5z1xminb	144	36	shown	show	VBN
cord-299281-5z1xminb	144	37	)	)	-RRB-
cord-299281-5z1xminb	144	38	.	.	.
cord-299281-5z1xminb	145	1	Because	because	IN
cord-299281-5z1xminb	145	2	the	the	DT
cord-299281-5z1xminb	145	3	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	145	4	material	material	NN
cord-299281-5z1xminb	145	5	was	be	VBD
cord-299281-5z1xminb	145	6	easiest	easy	JJS
cord-299281-5z1xminb	145	7	to	to	TO
cord-299281-5z1xminb	145	8	detect	detect	VB
cord-299281-5z1xminb	145	9	in	in	IN
cord-299281-5z1xminb	145	10	HeLa	HeLa	NNP
cord-299281-5z1xminb	145	11	cells	cell	NNS
cord-299281-5z1xminb	145	12	,	,	,
cord-299281-5z1xminb	145	13	we	-PRON-	PRP
cord-299281-5z1xminb	145	14	used	use	VBD
cord-299281-5z1xminb	145	15	these	these	DT
cord-299281-5z1xminb	145	16	cells	cell	NNS
cord-299281-5z1xminb	145	17	for	for	IN
cord-299281-5z1xminb	145	18	subsequent	subsequent	JJ
cord-299281-5z1xminb	145	19	experiments	experiment	NNS
cord-299281-5z1xminb	145	20	.	.	.
cord-299281-5z1xminb	146	1	The	the	DT
cord-299281-5z1xminb	146	2	material	material	NN
cord-299281-5z1xminb	146	3	at	at	IN
cord-299281-5z1xminb	146	4	the	the	DT
cord-299281-5z1xminb	146	5	top	top	NN
cord-299281-5z1xminb	146	6	of	of	IN
cord-299281-5z1xminb	146	7	the	the	DT
cord-299281-5z1xminb	146	8	stacking	stacking	NN
cord-299281-5z1xminb	146	9	gel	gel	NN
cord-299281-5z1xminb	146	10	could	could	MD
cord-299281-5z1xminb	146	11	be	be	VB
cord-299281-5z1xminb	146	12	detected	detect	VBN
cord-299281-5z1xminb	146	13	after	after	IN
cord-299281-5z1xminb	146	14	transfer	transfer	NN
cord-299281-5z1xminb	146	15	to	to	IN
cord-299281-5z1xminb	146	16	nitrocellulose	nitrocellulose	NN
cord-299281-5z1xminb	146	17	and	and	CC
cord-299281-5z1xminb	146	18	probing	probe	VBG
cord-299281-5z1xminb	146	19	with	with	IN
cord-299281-5z1xminb	146	20	anti	anti	JJ
cord-299281-5z1xminb	146	21	-	-	JJ
cord-299281-5z1xminb	146	22	VSV	VSV	NNP
cord-299281-5z1xminb	146	23	G	G	NNP
cord-299281-5z1xminb	146	24	antibodies	antibody	NNS
cord-299281-5z1xminb	146	25	by	by	IN
cord-299281-5z1xminb	146	26	immunoblotting	immunoblotte	VBG
cord-299281-5z1xminb	146	27	,	,	,
cord-299281-5z1xminb	146	28	suggesting	suggest	VBG
cord-299281-5z1xminb	146	29	that	that	IN
cord-299281-5z1xminb	146	30	it	-PRON-	PRP
cord-299281-5z1xminb	146	31	contained	contain	VBD
cord-299281-5z1xminb	146	32	Gml	Gml	NNP
cord-299281-5z1xminb	146	33	(	(	-LRB-
cord-299281-5z1xminb	146	34	or	or	CC
cord-299281-5z1xminb	146	35	the	the	DT
cord-299281-5z1xminb	146	36	related	related	JJ
cord-299281-5z1xminb	146	37	constructs	construct	NNS
cord-299281-5z1xminb	146	38	;	;	:
cord-299281-5z1xminb	146	39	data	datum	NNS
cord-299281-5z1xminb	146	40	not	not	RB
cord-299281-5z1xminb	146	41	shown	show	VBN
cord-299281-5z1xminb	146	42	)	)	-RRB-
cord-299281-5z1xminb	146	43	.	.	.
cord-299281-5z1xminb	147	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	147	2	,	,	,
cord-299281-5z1xminb	147	3	this	this	DT
cord-299281-5z1xminb	147	4	large	large	JJ
cord-299281-5z1xminb	147	5	species	specie	NNS
cord-299281-5z1xminb	147	6	was	be	VBD
cord-299281-5z1xminb	147	7	efficiently	efficiently	RB
cord-299281-5z1xminb	147	8	precipitated	precipitate	VBN
cord-299281-5z1xminb	147	9	by	by	IN
cord-299281-5z1xminb	147	10	several	several	JJ
cord-299281-5z1xminb	147	11	conformationsensitive	conformationsensitive	JJ
cord-299281-5z1xminb	147	12	anti	anti	JJ
cord-299281-5z1xminb	147	13	-	-	JJ
cord-299281-5z1xminb	147	14	VSV	VSV	NNP
cord-299281-5z1xminb	147	15	G	G	NNP
cord-299281-5z1xminb	147	16	monoclonal	monoclonal	JJ
cord-299281-5z1xminb	147	17	antibodies	antibody	NNS
cord-299281-5z1xminb	147	18	that	that	WDT
cord-299281-5z1xminb	147	19	do	do	VBP
cord-299281-5z1xminb	147	20	not	not	RB
cord-299281-5z1xminb	147	21	recognize	recognize	VB
cord-299281-5z1xminb	147	22	grossly	grossly	RB
cord-299281-5z1xminb	147	23	misfolded	misfolde	VBN
cord-299281-5z1xminb	147	24	proteins	protein	NNS
cord-299281-5z1xminb	147	25	(	(	-LRB-
cord-299281-5z1xminb	148	1	Doms	Doms	NNP
cord-299281-5z1xminb	148	2	et	et	FW
cord-299281-5z1xminb	148	3	ai	ai	NNP
cord-299281-5z1xminb	148	4	.	.	NNP
cord-299281-5z1xminb	148	5	,	,	,
cord-299281-5z1xminb	148	6	1988	1988	CD
cord-299281-5z1xminb	148	7	)	)	-RRB-
cord-299281-5z1xminb	148	8	.	.	.
cord-299281-5z1xminb	149	1	We	-PRON-	PRP
cord-299281-5z1xminb	149	2	tried	try	VBD
cord-299281-5z1xminb	149	3	to	to	TO
cord-299281-5z1xminb	149	4	solubilize	solubilize	VB
cord-299281-5z1xminb	149	5	the	the	DT
cord-299281-5z1xminb	149	6	Gml	Gml	NNP
cord-299281-5z1xminb	149	7	oligomer	oligomer	XX
cord-299281-5z1xminb	149	8	using	use	VBG
cord-299281-5z1xminb	149	9	a	a	DT
cord-299281-5z1xminb	149	10	wide	wide	JJ
cord-299281-5z1xminb	149	11	variety	variety	NN
cord-299281-5z1xminb	149	12	of	of	IN
cord-299281-5z1xminb	149	13	lysis	lysis	NN
cord-299281-5z1xminb	149	14	conditions	condition	NNS
cord-299281-5z1xminb	149	15	and	and	CC
cord-299281-5z1xminb	149	16	sample	sample	NN
cord-299281-5z1xminb	149	17	treatments	treatment	NNS
cord-299281-5z1xminb	149	18	.	.	.
cord-299281-5z1xminb	150	1	None	none	NN
cord-299281-5z1xminb	150	2	of	of	IN
cord-299281-5z1xminb	150	3	the	the	DT
cord-299281-5z1xminb	150	4	modifications	modification	NNS
cord-299281-5z1xminb	150	5	we	-PRON-	PRP
cord-299281-5z1xminb	150	6	tested	test	VBD
cord-299281-5z1xminb	150	7	,	,	,
cord-299281-5z1xminb	150	8	including	include	VBG
cord-299281-5z1xminb	150	9	changes	change	NNS
cord-299281-5z1xminb	150	10	in	in	IN
cord-299281-5z1xminb	150	11	salt	salt	NN
cord-299281-5z1xminb	150	12	concentration	concentration	NN
cord-299281-5z1xminb	150	13	,	,	,
cord-299281-5z1xminb	150	14	reducing	reduce	VBG
cord-299281-5z1xminb	150	15	agents	agent	NNS
cord-299281-5z1xminb	150	16	,	,	,
cord-299281-5z1xminb	150	17	detergents	detergent	NNS
cord-299281-5z1xminb	150	18	,	,	,
cord-299281-5z1xminb	150	19	chaotropic	chaotropic	JJ
cord-299281-5z1xminb	150	20	agents	agent	NNS
cord-299281-5z1xminb	150	21	(	(	-LRB-
cord-299281-5z1xminb	150	22	e.g.	e.g.	RB
cord-299281-5z1xminb	150	23	,	,	,
cord-299281-5z1xminb	150	24	urea	urea	NN
cord-299281-5z1xminb	150	25	,	,	,
cord-299281-5z1xminb	150	26	guanidinium	guanidinium	NNP
cord-299281-5z1xminb	150	27	HC1	HC1	NNP
cord-299281-5z1xminb	150	28	)	)	-RRB-
cord-299281-5z1xminb	150	29	,	,	,
cord-299281-5z1xminb	150	30	or	or	CC
cord-299281-5z1xminb	150	31	temperature	temperature	NN
cord-299281-5z1xminb	150	32	of	of	IN
cord-299281-5z1xminb	150	33	solubilization	solubilization	NN
cord-299281-5z1xminb	150	34	or	or	CC
cord-299281-5z1xminb	150	35	sample	sample	NN
cord-299281-5z1xminb	150	36	elution	elution	NN
cord-299281-5z1xminb	150	37	disrupted	disrupt	VBD
cord-299281-5z1xminb	150	38	the	the	DT
cord-299281-5z1xminb	150	39	SDS	SDS	NNP
cord-299281-5z1xminb	150	40	-	-	HYPH
cord-299281-5z1xminb	150	41	resistant	resistant	JJ
cord-299281-5z1xminb	150	42	oligomer	oligomer	NNP
cord-299281-5z1xminb	150	43	(	(	-LRB-
cord-299281-5z1xminb	150	44	data	datum	NNS
cord-299281-5z1xminb	150	45	not	not	RB
cord-299281-5z1xminb	150	46	shown	show	VBN
cord-299281-5z1xminb	150	47	)	)	-RRB-
cord-299281-5z1xminb	150	48	.	.	.
cord-299281-5z1xminb	151	1	A	a	DT
cord-299281-5z1xminb	151	2	very	very	RB
cord-299281-5z1xminb	151	3	small	small	JJ
cord-299281-5z1xminb	151	4	amount	amount	NN
cord-299281-5z1xminb	151	5	of	of	IN
cord-299281-5z1xminb	151	6	SDS	sds	NN
cord-299281-5z1xminb	151	7	-	-	HYPH
cord-299281-5z1xminb	151	8	sensitive	sensitive	JJ
cord-299281-5z1xminb	151	9	Gml	Gml	NNP
cord-299281-5z1xminb	151	10	could	could	MD
cord-299281-5z1xminb	151	11	be	be	VB
cord-299281-5z1xminb	151	12	produced	produce	VBN
cord-299281-5z1xminb	151	13	when	when	WRB
cord-299281-5z1xminb	151	14	the	the	DT
cord-299281-5z1xminb	151	15	top	top	NN
cord-299281-5z1xminb	151	16	of	of	IN
cord-299281-5z1xminb	151	17	the	the	DT
cord-299281-5z1xminb	151	18	stacking	stacking	NN
cord-299281-5z1xminb	151	19	gel	gel	NN
cord-299281-5z1xminb	151	20	(	(	-LRB-
cord-299281-5z1xminb	151	21	containing	contain	VBG
cord-299281-5z1xminb	151	22	the	the	DT
cord-299281-5z1xminb	151	23	SDS	SDS	NNP
cord-299281-5z1xminb	151	24	-	-	HYPH
cord-299281-5z1xminb	151	25	resistant	resistant	JJ
cord-299281-5z1xminb	151	26	oligomer	oligomer	NNP
cord-299281-5z1xminb	151	27	)	)	-RRB-
cord-299281-5z1xminb	151	28	was	be	VBD
cord-299281-5z1xminb	151	29	isolated	isolate	VBN
cord-299281-5z1xminb	151	30	after	after	IN
cord-299281-5z1xminb	151	31	electrophoresis	electrophoresis	NN
cord-299281-5z1xminb	151	32	,	,	,
cord-299281-5z1xminb	151	33	placed	place	VBN
cord-299281-5z1xminb	151	34	in	in	IN
cord-299281-5z1xminb	151	35	the	the	DT
cord-299281-5z1xminb	151	36	well	well	NN
cord-299281-5z1xminb	151	37	of	of	IN
cord-299281-5z1xminb	151	38	a	a	DT
cord-299281-5z1xminb	151	39	fresh	fresh	JJ
cord-299281-5z1xminb	151	40	gel	gel	NN
cord-299281-5z1xminb	151	41	,	,	,
cord-299281-5z1xminb	151	42	and	and	CC
cord-299281-5z1xminb	151	43	reelectrophoresed	reelectrophoresed	JJ
cord-299281-5z1xminb	151	44	(	(	-LRB-
cord-299281-5z1xminb	151	45	not	not	RB
cord-299281-5z1xminb	151	46	shown	show	VBN
cord-299281-5z1xminb	151	47	)	)	-RRB-
cord-299281-5z1xminb	151	48	.	.	.
cord-299281-5z1xminb	152	1	Together	together	RB
cord-299281-5z1xminb	152	2	,	,	,
cord-299281-5z1xminb	152	3	these	these	DT
cord-299281-5z1xminb	152	4	observations	observation	NNS
cord-299281-5z1xminb	152	5	suggest	suggest	VBP
cord-299281-5z1xminb	152	6	that	that	IN
cord-299281-5z1xminb	152	7	SDS	SDS	NNP
cord-299281-5z1xminb	152	8	-	-	HYPH
cord-299281-5z1xminb	152	9	resistant	resistant	JJ
cord-299281-5z1xminb	152	10	oligomer	oligomer	NNP
cord-299281-5z1xminb	152	11	formation	formation	NN
cord-299281-5z1xminb	152	12	is	be	VBZ
cord-299281-5z1xminb	152	13	an	an	DT
cord-299281-5z1xminb	152	14	intrinsic	intrinsic	JJ
cord-299281-5z1xminb	152	15	property	property	NN
cord-299281-5z1xminb	152	16	of	of	IN
cord-299281-5z1xminb	152	17	Gml	Gml	NNP
cord-299281-5z1xminb	152	18	and	and	CC
cord-299281-5z1xminb	152	19	mutant	mutant	JJ
cord-299281-5z1xminb	152	20	Gml	Gml	NNP
cord-299281-5z1xminb	152	21	proteins	protein	NNS
cord-299281-5z1xminb	152	22	that	that	WDT
cord-299281-5z1xminb	152	23	are	be	VBP
cord-299281-5z1xminb	152	24	retained	retain	VBN
cord-299281-5z1xminb	152	25	in	in	IN
cord-299281-5z1xminb	152	26	the	the	DT
cord-299281-5z1xminb	152	27	Golgi	Golgi	NNP
cord-299281-5z1xminb	152	28	complex	complex	NN
cord-299281-5z1xminb	152	29	,	,	,
cord-299281-5z1xminb	152	30	but	but	CC
cord-299281-5z1xminb	152	31	not	not	RB
cord-299281-5z1xminb	152	32	of	of	IN
cord-299281-5z1xminb	152	33	mutants	mutant	NNS
cord-299281-5z1xminb	152	34	that	that	WDT
cord-299281-5z1xminb	152	35	efficiently	efficiently	RB
cord-299281-5z1xminb	152	36	reach	reach	VBP
cord-299281-5z1xminb	152	37	the	the	DT
cord-299281-5z1xminb	152	38	plasma	plasma	NN
cord-299281-5z1xminb	152	39	membrane	membrane	NN
cord-299281-5z1xminb	152	40	.	.	.
cord-299281-5z1xminb	152	41	 	 	_SP
cord-299281-5z1xminb	153	1	If	if	IN
cord-299281-5z1xminb	153	2	the	the	DT
cord-299281-5z1xminb	153	3	oligomerized	oligomerize	VBN
cord-299281-5z1xminb	153	4	protein	protein	NN
cord-299281-5z1xminb	153	5	represents	represent	VBZ
cord-299281-5z1xminb	153	6	a	a	DT
cord-299281-5z1xminb	153	7	distinct	distinct	JJ
cord-299281-5z1xminb	153	8	intracellular	intracellular	JJ
cord-299281-5z1xminb	153	9	pool	pool	NN
cord-299281-5z1xminb	153	10	of	of	IN
cord-299281-5z1xminb	153	11	protein(s	protein(s	NNP
cord-299281-5z1xminb	153	12	)	)	-RRB-
cord-299281-5z1xminb	153	13	,	,	,
cord-299281-5z1xminb	153	14	it	-PRON-	PRP
cord-299281-5z1xminb	153	15	should	should	MD
cord-299281-5z1xminb	153	16	not	not	RB
cord-299281-5z1xminb	153	17	be	be	VB
cord-299281-5z1xminb	153	18	susceptible	susceptible	JJ
cord-299281-5z1xminb	153	19	to	to	IN
cord-299281-5z1xminb	153	20	cell	cell	NN
cord-299281-5z1xminb	153	21	surface	surface	NN
cord-299281-5z1xminb	153	22	biotinylation	biotinylation	NN
cord-299281-5z1xminb	153	23	.	.	.
cord-299281-5z1xminb	154	1	Moreover	moreover	RB
cord-299281-5z1xminb	154	2	,	,	,
cord-299281-5z1xminb	154	3	we	-PRON-	PRP
cord-299281-5z1xminb	154	4	predicted	predict	VBD
cord-299281-5z1xminb	154	5	that	that	IN
cord-299281-5z1xminb	154	6	an	an	DT
cord-299281-5z1xminb	154	7	oligomer	oligomer	NN
cord-299281-5z1xminb	154	8	that	that	WDT
cord-299281-5z1xminb	154	9	formed	form	VBN
cord-299281-5z1xminb	154	10	nonspecifically	nonspecifically	RB
cord-299281-5z1xminb	154	11	after	after	IN
cord-299281-5z1xminb	154	12	cell	cell	NN
cord-299281-5z1xminb	154	13	solubilization	solubilization	NN
cord-299281-5z1xminb	154	14	would	would	MD
cord-299281-5z1xminb	154	15	be	be	VB
cord-299281-5z1xminb	154	16	precipitated	precipitate	VBN
cord-299281-5z1xminb	154	17	by	by	IN
cord-299281-5z1xminb	154	18	streptavidin	streptavidin	NNS
cord-299281-5z1xminb	154	19	since	since	IN
cord-299281-5z1xminb	154	20	incorporation	incorporation	NN
cord-299281-5z1xminb	154	21	of	of	IN
cord-299281-5z1xminb	154	22	a	a	DT
cord-299281-5z1xminb	154	23	few	few	JJ
cord-299281-5z1xminb	154	24	biotinylated	biotinylated	JJ
cord-299281-5z1xminb	154	25	molecules	molecule	NNS
cord-299281-5z1xminb	154	26	would	would	MD
cord-299281-5z1xminb	154	27	allow	allow	VB
cord-299281-5z1xminb	154	28	precipitation	precipitation	NN
cord-299281-5z1xminb	154	29	of	of	IN
cord-299281-5z1xminb	154	30	the	the	DT
cord-299281-5z1xminb	154	31	entire	entire	JJ
cord-299281-5z1xminb	154	32	oligomer	oligomer	NN
cord-299281-5z1xminb	154	33	.	.	.
cord-299281-5z1xminb	155	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	155	2	cells	cell	NNS
cord-299281-5z1xminb	155	3	expressing	express	VBG
cord-299281-5z1xminb	155	4	Gml	Gml	NNP
cord-299281-5z1xminb	155	5	,	,	,
cord-299281-5z1xminb	155	6	VSV	VSV	NNP
cord-299281-5z1xminb	155	7	G	G	NNP
cord-299281-5z1xminb	155	8	,	,	,
cord-299281-5z1xminb	155	9	or	or	CC
cord-299281-5z1xminb	155	10	Gmlin	Gmlin	NNP
cord-299281-5z1xminb	155	11	,	,	,
cord-299281-5z1xminb	155	12	were	be	VBD
cord-299281-5z1xminb	155	13	metabolically	metabolically	RB
cord-299281-5z1xminb	155	14	labeled	label	VBN
cord-299281-5z1xminb	155	15	for	for	IN
cord-299281-5z1xminb	155	16	15	15	CD
cord-299281-5z1xminb	155	17	min	min	NN
cord-299281-5z1xminb	155	18	,	,	,
cord-299281-5z1xminb	155	19	and	and	CC
cord-299281-5z1xminb	155	20	then	then	RB
cord-299281-5z1xminb	155	21	chased	chase	VBD
cord-299281-5z1xminb	155	22	for	for	IN
cord-299281-5z1xminb	155	23	45	45	CD
cord-299281-5z1xminb	155	24	min	min	NN
cord-299281-5z1xminb	155	25	before	before	IN
cord-299281-5z1xminb	155	26	cell	cell	NN
cord-299281-5z1xminb	155	27	-	-	HYPH
cord-299281-5z1xminb	155	28	surface	surface	NN
cord-299281-5z1xminb	155	29	biotinylation	biotinylation	NN
cord-299281-5z1xminb	155	30	with	with	IN
cord-299281-5z1xminb	155	31	sulfo	sulfo	NN
cord-299281-5z1xminb	155	32	-	-	HYPH
cord-299281-5z1xminb	155	33	NHSbiotin	NHSbiotin	NNS
cord-299281-5z1xminb	155	34	.	.	.
cord-299281-5z1xminb	156	1	After	after	IN
cord-299281-5z1xminb	156	2	immunoprecipitation	immunoprecipitation	NN
cord-299281-5z1xminb	156	3	,	,	,
cord-299281-5z1xminb	156	4	an	an	DT
cord-299281-5z1xminb	156	5	aliquot	aliquot	NN
cord-299281-5z1xminb	156	6	of	of	IN
cord-299281-5z1xminb	156	7	each	each	DT
cord-299281-5z1xminb	156	8	sample	sample	NN
cord-299281-5z1xminb	156	9	was	be	VBD
cord-299281-5z1xminb	156	10	treated	treat	VBN
cord-299281-5z1xminb	156	11	with	with	IN
cord-299281-5z1xminb	156	12	streptavidin	streptavidin	NN
cord-299281-5z1xminb	156	13	-	-	HYPH
cord-299281-5z1xminb	156	14	coupled	couple	VBN
cord-299281-5z1xminb	156	15	agarose	agarose	NN
cord-299281-5z1xminb	156	16	,	,	,
cord-299281-5z1xminb	156	17	and	and	CC
cord-299281-5z1xminb	156	18	biotinylated	biotinylated	JJ
cord-299281-5z1xminb	156	19	and	and	CC
cord-299281-5z1xminb	156	20	non	non	JJ
cord-299281-5z1xminb	156	21	-	-	JJ
cord-299281-5z1xminb	156	22	biotinylated	biotinylated	JJ
cord-299281-5z1xminb	156	23	proteins	protein	NNS
cord-299281-5z1xminb	156	24	were	be	VBD
cord-299281-5z1xminb	156	25	recovered	recover	VBN
cord-299281-5z1xminb	156	26	and	and	CC
cord-299281-5z1xminb	156	27	analyzed	analyze	VBN
cord-299281-5z1xminb	156	28	by	by	IN
cord-299281-5z1xminb	156	29	SDS	SDS	NNP
cord-299281-5z1xminb	156	30	-	-	HYPH
cord-299281-5z1xminb	156	31	PAGE	PAGE	NNP
cord-299281-5z1xminb	156	32	(	(	-LRB-
cord-299281-5z1xminb	156	33	Fig	Fig	NNP
cord-299281-5z1xminb	156	34	.	.	NNP
cord-299281-5z1xminb	156	35	2	2	CD
cord-299281-5z1xminb	156	36	)	)	-RRB-
cord-299281-5z1xminb	156	37	.	.	.
cord-299281-5z1xminb	157	1	Under	under	IN
cord-299281-5z1xminb	157	2	these	these	DT
cord-299281-5z1xminb	157	3	conditions	condition	NNS
cord-299281-5z1xminb	157	4	,	,	,
cord-299281-5z1xminb	157	5	,	,	,
cord-299281-5z1xminb	157	6	o50	o50	CD
cord-299281-5z1xminb	157	7	%	%	NN
cord-299281-5z1xminb	157	8	of	of	IN
cord-299281-5z1xminb	157	9	the	the	DT
cord-299281-5z1xminb	157	10	total	total	JJ
cord-299281-5z1xminb	157	11	VSV	VSV	NNP
cord-299281-5z1xminb	157	12	G	G	NNP
cord-299281-5z1xminb	157	13	was	be	VBD
cord-299281-5z1xminb	157	14	biotinylated	biotinylate	VBN
cord-299281-5z1xminb	157	15	and	and	CC
cord-299281-5z1xminb	157	16	could	could	MD
cord-299281-5z1xminb	157	17	be	be	VB
cord-299281-5z1xminb	157	18	recovered	recover	VBN
cord-299281-5z1xminb	157	19	in	in	IN
cord-299281-5z1xminb	157	20	the	the	DT
cord-299281-5z1xminb	157	21	streptavidin	streptavidin	NNP
cord-299281-5z1xminb	157	22	pellet	pellet	NN
cord-299281-5z1xminb	157	23	.	.	.
cord-299281-5z1xminb	158	1	Only	only	RB
cord-299281-5z1xminb	158	2	the	the	DT
cord-299281-5z1xminb	158	3	mature	mature	JJ
cord-299281-5z1xminb	158	4	(	(	-LRB-
cord-299281-5z1xminb	158	5	sialylated	sialylated	JJ
cord-299281-5z1xminb	158	6	)	)	-RRB-
cord-299281-5z1xminb	158	7	form	form	NN
cord-299281-5z1xminb	158	8	of	of	IN
cord-299281-5z1xminb	158	9	VSV	VSV	NNP
cord-299281-5z1xminb	158	10	G	G	NNP
cord-299281-5z1xminb	158	11	was	be	VBD
cord-299281-5z1xminb	158	12	recovered	recover	VBN
cord-299281-5z1xminb	158	13	in	in	IN
cord-299281-5z1xminb	158	14	this	this	DT
cord-299281-5z1xminb	158	15	fraction	fraction	NN
cord-299281-5z1xminb	158	16	,	,	,
cord-299281-5z1xminb	158	17	suggesting	suggest	VBG
cord-299281-5z1xminb	158	18	that	that	IN
cord-299281-5z1xminb	158	19	biotinylation	biotinylation	NN
cord-299281-5z1xminb	158	20	was	be	VBD
cord-299281-5z1xminb	158	21	indeed	indeed	RB
cord-299281-5z1xminb	158	22	restricted	restrict	VBN
cord-299281-5z1xminb	158	23	to	to	IN
cord-299281-5z1xminb	158	24	cell	cell	NN
cord-299281-5z1xminb	158	25	surface	surface	NN
cord-299281-5z1xminb	158	26	proteins	protein	NNS
cord-299281-5z1xminb	158	27	.	.	.
cord-299281-5z1xminb	159	1	In	in	IN
cord-299281-5z1xminb	159	2	contrast	contrast	NN
cord-299281-5z1xminb	159	3	,	,	,
cord-299281-5z1xminb	159	4	essentially	essentially	RB
cord-299281-5z1xminb	159	5	none	none	NN
cord-299281-5z1xminb	159	6	of	of	IN
cord-299281-5z1xminb	159	7	the	the	DT
cord-299281-5z1xminb	159	8	Gml	Gml	NNP
cord-299281-5z1xminb	159	9	was	be	VBD
cord-299281-5z1xminb	159	10	precipitated	precipitate	VBN
cord-299281-5z1xminb	159	11	by	by	IN
cord-299281-5z1xminb	159	12	streptavidin	streptavidin	NNP
cord-299281-5z1xminb	159	13	,	,	,
cord-299281-5z1xminb	159	14	suggesting	suggest	VBG
cord-299281-5z1xminb	159	15	that	that	IN
cord-299281-5z1xminb	159	16	this	this	DT
cord-299281-5z1xminb	159	17	protein	protein	NN
cord-299281-5z1xminb	159	18	remained	remain	VBD
cord-299281-5z1xminb	159	19	in	in	IN
cord-299281-5z1xminb	159	20	an	an	DT
cord-299281-5z1xminb	159	21	intracellular	intracellular	JJ
cord-299281-5z1xminb	159	22	compartment	compartment	NN
cord-299281-5z1xminb	159	23	.	.	.
cord-299281-5z1xminb	160	1	A	a	DT
cord-299281-5z1xminb	160	2	small	small	JJ
cord-299281-5z1xminb	160	3	amount	amount	NN
cord-299281-5z1xminb	160	4	of	of	IN
cord-299281-5z1xminb	160	5	SDS	sds	NN
cord-299281-5z1xminb	160	6	-	-	HYPH
cord-299281-5z1xminb	160	7	sensitive	sensitive	JJ
cord-299281-5z1xminb	160	8	Gmli~	gmli~	NN
cord-299281-5z1xminb	160	9	,	,	,
cord-299281-5z1xminb	160	10	was	be	VBD
cord-299281-5z1xminb	160	11	recovered	recover	VBN
cord-299281-5z1xminb	160	12	in	in	IN
cord-299281-5z1xminb	160	13	the	the	DT
cord-299281-5z1xminb	160	14	streptavidin	streptavidin	NNP
cord-299281-5z1xminb	160	15	pellet	pellet	NN
cord-299281-5z1xminb	160	16	.	.	.
cord-299281-5z1xminb	161	1	More	more	RBR
cord-299281-5z1xminb	161	2	importantly	importantly	RB
cord-299281-5z1xminb	161	3	,	,	,
cord-299281-5z1xminb	161	4	no	no	DT
cord-299281-5z1xminb	161	5	SDS	sds	NN
cord-299281-5z1xminb	161	6	-	-	HYPH
cord-299281-5z1xminb	161	7	resistant	resistant	JJ
cord-299281-5z1xminb	161	8	material	material	NN
cord-299281-5z1xminb	161	9	was	be	VBD
cord-299281-5z1xminb	161	10	biotinylated	biotinylate	VBN
cord-299281-5z1xminb	161	11	,	,	,
cord-299281-5z1xminb	161	12	suggesting	suggest	VBG
cord-299281-5z1xminb	161	13	that	that	IN
cord-299281-5z1xminb	161	14	the	the	DT
cord-299281-5z1xminb	161	15	SDS	SDS	NNP
cord-299281-5z1xminb	161	16	-	-	HYPH
cord-299281-5z1xminb	161	17	resistant	resistant	JJ
cord-299281-5z1xminb	161	18	Gmli~	gmli~	NN
cord-299281-5z1xminb	161	19	constitutes	constitute	VBZ
cord-299281-5z1xminb	161	20	an	an	DT
cord-299281-5z1xminb	161	21	intracellular	intracellular	JJ
cord-299281-5z1xminb	161	22	(	(	-LRB-
cord-299281-5z1xminb	161	23	perhaps	perhaps	RB
cord-299281-5z1xminb	161	24	Golgi	golgi	NN
cord-299281-5z1xminb	161	25	-	-	HYPH
cord-299281-5z1xminb	161	26	resident	resident	NN
cord-299281-5z1xminb	161	27	)	)	-RRB-
cord-299281-5z1xminb	161	28	pool	pool	NN
cord-299281-5z1xminb	161	29	that	that	WDT
cord-299281-5z1xminb	161	30	is	be	VBZ
cord-299281-5z1xminb	161	31	distinct	distinct	JJ
cord-299281-5z1xminb	161	32	from	from	IN
cord-299281-5z1xminb	161	33	the	the	DT
cord-299281-5z1xminb	161	34	SDS	SDS	NNP
cord-299281-5z1xminb	161	35	-	-	HYPH
cord-299281-5z1xminb	161	36	sensitive	sensitive	JJ
cord-299281-5z1xminb	161	37	protein	protein	NN
cord-299281-5z1xminb	161	38	,	,	,
cord-299281-5z1xminb	161	39	and	and	CC
cord-299281-5z1xminb	161	40	that	that	IN
cord-299281-5z1xminb	161	41	only	only	RB
cord-299281-5z1xminb	161	42	a	a	DT
cord-299281-5z1xminb	161	43	portion	portion	NN
cord-299281-5z1xminb	161	44	of	of	IN
cord-299281-5z1xminb	161	45	Gml~	gml~	NN
cord-299281-5z1xminb	161	46	,	,	,
cord-299281-5z1xminb	161	47	is	be	VBZ
cord-299281-5z1xminb	161	48	transported	transport	VBN
cord-299281-5z1xminb	161	49	to	to	IN
cord-299281-5z1xminb	161	50	the	the	DT
cord-299281-5z1xminb	161	51	plasma	plasma	NN
cord-299281-5z1xminb	161	52	membrane	membrane	NN
cord-299281-5z1xminb	161	53	where	where	WRB
cord-299281-5z1xminb	161	54	it	-PRON-	PRP
cord-299281-5z1xminb	161	55	can	can	MD
cord-299281-5z1xminb	161	56	be	be	VB
cord-299281-5z1xminb	161	57	biotinylated	biotinylate	VBN
cord-299281-5z1xminb	161	58	.	.	.
cord-299281-5z1xminb	162	1	This	this	DT
cord-299281-5z1xminb	162	2	result	result	NN
cord-299281-5z1xminb	162	3	strongly	strongly	RB
cord-299281-5z1xminb	162	4	suggested	suggest	VBD
cord-299281-5z1xminb	162	5	that	that	IN
cord-299281-5z1xminb	162	6	oligomer	oligomer	NNP
cord-299281-5z1xminb	162	7	formation	formation	NN
cord-299281-5z1xminb	162	8	occurs	occur	VBZ
cord-299281-5z1xminb	162	9	before	before	IN
cord-299281-5z1xminb	162	10	cell	cell	NN
cord-299281-5z1xminb	162	11	solubilization	solubilization	NN
cord-299281-5z1xminb	162	12	.	.	.
cord-299281-5z1xminb	163	1	The	the	DT
cord-299281-5z1xminb	163	2	time	time	NN
cord-299281-5z1xminb	163	3	course	course	NN
cord-299281-5z1xminb	163	4	of	of	IN
cord-299281-5z1xminb	163	5	Gml	Gml	NNP
cord-299281-5z1xminb	163	6	oligomerization	oligomerization	NN
cord-299281-5z1xminb	163	7	was	be	VBD
cord-299281-5z1xminb	163	8	determined	determine	VBN
cord-299281-5z1xminb	163	9	.	.	.
cord-299281-5z1xminb	164	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	164	2	cells	cell	NNS
cord-299281-5z1xminb	164	3	expressing	express	VBG
cord-299281-5z1xminb	164	4	Gml	Gml	NNP
cord-299281-5z1xminb	164	5	were	be	VBD
cord-299281-5z1xminb	164	6	metabolically	metabolically	RB
cord-299281-5z1xminb	164	7	labeled	label	VBN
cord-299281-5z1xminb	164	8	for	for	IN
cord-299281-5z1xminb	164	9	5	5	CD
cord-299281-5z1xminb	164	10	min	min	NN
cord-299281-5z1xminb	164	11	,	,	,
cord-299281-5z1xminb	164	12	chased	chase	VBN
cord-299281-5z1xminb	164	13	for	for	IN
cord-299281-5z1xminb	164	14	the	the	DT
cord-299281-5z1xminb	164	15	indicated	indicate	VBN
cord-299281-5z1xminb	164	16	times	time	NNS
cord-299281-5z1xminb	164	17	,	,	,
cord-299281-5z1xminb	164	18	solubilized	solubilized	JJ
cord-299281-5z1xminb	164	19	,	,	,
cord-299281-5z1xminb	164	20	and	and	CC
cord-299281-5z1xminb	164	21	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	164	22	with	with	IN
cord-299281-5z1xminb	164	23	anti	anti	JJ
cord-299281-5z1xminb	164	24	-	-	JJ
cord-299281-5z1xminb	164	25	VSV	VSV	NNP
cord-299281-5z1xminb	164	26	G	G	NNP
cord-299281-5z1xminb	164	27	antibody	antibody	NN
cord-299281-5z1xminb	164	28	(	(	-LRB-
cord-299281-5z1xminb	164	29	Fig	Fig	NNP
cord-299281-5z1xminb	164	30	.	.	.
cord-299281-5z1xminb	164	31	3	3	CD
cord-299281-5z1xminb	164	32	)	)	-RRB-
cord-299281-5z1xminb	164	33	.	.	.
cord-299281-5z1xminb	165	1	Oligo-	Oligo-	NNP
cord-299281-5z1xminb	166	1	Figure	figure	NN
cord-299281-5z1xminb	166	2	2	2	CD
cord-299281-5z1xminb	166	3	.	.	.
cord-299281-5z1xminb	167	1	SDS	sds	NN
cord-299281-5z1xminb	167	2	-	-	HYPH
cord-299281-5z1xminb	167	3	resistant	resistant	JJ
cord-299281-5z1xminb	167	4	oligomers	oligomer	NNS
cord-299281-5z1xminb	167	5	are	be	VBP
cord-299281-5z1xminb	167	6	not	not	RB
cord-299281-5z1xminb	167	7	subject	subject	JJ
cord-299281-5z1xminb	167	8	to	to	IN
cord-299281-5z1xminb	167	9	cell	cell	NN
cord-299281-5z1xminb	167	10	surface	surface	NN
cord-299281-5z1xminb	167	11	biotinylation	biotinylation	NN
cord-299281-5z1xminb	167	12	.	.	.
cord-299281-5z1xminb	168	1	Ceils	Ceils	NNP
cord-299281-5z1xminb	168	2	were	be	VBD
cord-299281-5z1xminb	168	3	metabolically	metabolically	RB
cord-299281-5z1xminb	168	4	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	168	5	for	for	IN
cord-299281-5z1xminb	168	6	15	15	CD
cord-299281-5z1xminb	168	7	min	min	NN
cord-299281-5z1xminb	168	8	,	,	,
cord-299281-5z1xminb	168	9	and	and	CC
cord-299281-5z1xminb	168	10	then	then	RB
cord-299281-5z1xminb	168	11	chased	chase	VBD
cord-299281-5z1xminb	168	12	for	for	IN
cord-299281-5z1xminb	168	13	60	60	CD
cord-299281-5z1xminb	168	14	rain	rain	NN
cord-299281-5z1xminb	168	15	.	.	.
cord-299281-5z1xminb	169	1	Cell	cell	NN
cord-299281-5z1xminb	169	2	surface	surface	NN
cord-299281-5z1xminb	169	3	-	-	HYPH
cord-299281-5z1xminb	169	4	specific	specific	JJ
cord-299281-5z1xminb	169	5	biotinylatiun	biotinylatiun	NN
cord-299281-5z1xminb	169	6	was	be	VBD
cord-299281-5z1xminb	169	7	performed	perform	VBN
cord-299281-5z1xminb	169	8	at	at	IN
cord-299281-5z1xminb	169	9	4"C	4"c	CD
cord-299281-5z1xminb	169	10	using	use	VBG
cord-299281-5z1xminb	169	11	sulfo	sulfo	NN
cord-299281-5z1xminb	169	12	-	-	HYPH
cord-299281-5z1xminb	169	13	NHS	NHS	NNP
cord-299281-5z1xminb	169	14	-	-	HYPH
cord-299281-5z1xminb	169	15	biotin	biotin	NN
cord-299281-5z1xminb	169	16	,	,	,
cord-299281-5z1xminb	169	17	and	and	CC
cord-299281-5z1xminb	169	18	the	the	DT
cord-299281-5z1xminb	169	19	cells	cell	NNS
cord-299281-5z1xminb	169	20	were	be	VBD
cord-299281-5z1xminb	169	21	solubilized	solubilize	VBN
cord-299281-5z1xminb	169	22	and	and	CC
cord-299281-5z1xminb	169	23	immunopreeipitated	immunopreeipitate	VBN
cord-299281-5z1xminb	169	24	with	with	IN
cord-299281-5z1xminb	169	25	anti	anti	JJ
cord-299281-5z1xminb	169	26	-	-	JJ
cord-299281-5z1xminb	169	27	VSV	VSV	NNP
cord-299281-5z1xminb	169	28	antibody	antibody	NN
cord-299281-5z1xminb	169	29	.	.	.
cord-299281-5z1xminb	170	1	The	the	DT
cord-299281-5z1xminb	170	2	bound	bound	JJ
cord-299281-5z1xminb	170	3	protein	protein	NN
cord-299281-5z1xminb	170	4	was	be	VBD
cord-299281-5z1xminb	170	5	eluted	elute	VBN
cord-299281-5z1xminb	170	6	from	from	IN
cord-299281-5z1xminb	170	7	S.	S.	NNP
cord-299281-5z1xminb	170	8	aureus	aureus	NN
cord-299281-5z1xminb	170	9	pellets	pellet	NNS
cord-299281-5z1xminb	170	10	and	and	CC
cord-299281-5z1xminb	170	11	divided	divide	VBN
cord-299281-5z1xminb	170	12	in	in	IN
cord-299281-5z1xminb	170	13	half	half	NN
cord-299281-5z1xminb	170	14	.	.	.
cord-299281-5z1xminb	171	1	One	one	CD
cord-299281-5z1xminb	171	2	aliquot	aliquot	NN
cord-299281-5z1xminb	171	3	was	be	VBD
cord-299281-5z1xminb	171	4	solubilized	solubilize	VBN
cord-299281-5z1xminb	171	5	in	in	IN
cord-299281-5z1xminb	171	6	sample	sample	NN
cord-299281-5z1xminb	171	7	buffer	buffer	NN
cord-299281-5z1xminb	171	8	(	(	-LRB-
cord-299281-5z1xminb	171	9	T	T	NNP
cord-299281-5z1xminb	171	10	)	)	-RRB-
cord-299281-5z1xminb	171	11	,	,	,
cord-299281-5z1xminb	171	12	and	and	CC
cord-299281-5z1xminb	171	13	the	the	DT
cord-299281-5z1xminb	171	14	remainder	remainder	NN
cord-299281-5z1xminb	171	15	was	be	VBD
cord-299281-5z1xminb	171	16	incubated	incubate	VBN
cord-299281-5z1xminb	171	17	with	with	IN
cord-299281-5z1xminb	171	18	streptavidin	streptavidin	NN
cord-299281-5z1xminb	171	19	-	-	HYPH
cord-299281-5z1xminb	171	20	coupled	couple	VBN
cord-299281-5z1xminb	171	21	agarose	agarose	NN
cord-299281-5z1xminb	171	22	beads	bead	NNS
cord-299281-5z1xminb	171	23	.	.	.
cord-299281-5z1xminb	172	1	The	the	DT
cord-299281-5z1xminb	172	2	supematant	supematant	NN
cord-299281-5z1xminb	172	3	(	(	-LRB-
cord-299281-5z1xminb	172	4	IS	be	VBZ
cord-299281-5z1xminb	172	5	]	]	-RRB-
cord-299281-5z1xminb	172	6	,	,	,
cord-299281-5z1xminb	172	7	unbound	unbound	JJ
cord-299281-5z1xminb	172	8	material	material	NN
cord-299281-5z1xminb	172	9	)	)	-RRB-
cord-299281-5z1xminb	172	10	was	be	VBD
cord-299281-5z1xminb	172	11	collected	collect	VBN
cord-299281-5z1xminb	172	12	and	and	CC
cord-299281-5z1xminb	172	13	TCA	TCA	NNP
cord-299281-5z1xminb	172	14	precipitated	precipitate	VBD
cord-299281-5z1xminb	172	15	,	,	,
cord-299281-5z1xminb	172	16	and	and	CC
cord-299281-5z1xminb	172	17	the	the	DT
cord-299281-5z1xminb	172	18	streptavidin	streptavidin	NNP
cord-299281-5z1xminb	172	19	beads	bead	NNS
cord-299281-5z1xminb	172	20	(	(	-LRB-
cord-299281-5z1xminb	172	21	P	p	NN
cord-299281-5z1xminb	172	22	)	)	-RRB-
cord-299281-5z1xminb	172	23	were	be	VBD
cord-299281-5z1xminb	172	24	washed	wash	VBN
cord-299281-5z1xminb	172	25	and	and	CC
cord-299281-5z1xminb	172	26	solubilized	solubilize	VBN
cord-299281-5z1xminb	172	27	before	before	IN
cord-299281-5z1xminb	172	28	SDS	SDS	NNP
cord-299281-5z1xminb	172	29	-	-	HYPH
cord-299281-5z1xminb	172	30	gel	gel	NN
cord-299281-5z1xminb	172	31	electrophoresis	electrophoresis	NN
cord-299281-5z1xminb	172	32	.	.	.
cord-299281-5z1xminb	173	1	merization	merization	NNP
cord-299281-5z1xminb	173	2	was	be	VBD
cord-299281-5z1xminb	173	3	not	not	RB
cord-299281-5z1xminb	173	4	detected	detect	VBN
cord-299281-5z1xminb	173	5	immediately	immediately	RB
cord-299281-5z1xminb	173	6	after	after	IN
cord-299281-5z1xminb	173	7	synthesis	synthesis	NN
cord-299281-5z1xminb	173	8	of	of	IN
cord-299281-5z1xminb	173	9	Gml	Gml	NNP
cord-299281-5z1xminb	173	10	,	,	,
cord-299281-5z1xminb	173	11	but	but	CC
cord-299281-5z1xminb	173	12	occurred	occur	VBD
cord-299281-5z1xminb	173	13	gradually	gradually	RB
cord-299281-5z1xminb	173	14	with	with	IN
cord-299281-5z1xminb	173	15	a	a	DT
cord-299281-5z1xminb	173	16	lag	lag	NN
cord-299281-5z1xminb	173	17	of	of	IN
cord-299281-5z1xminb	173	18	~10	~10	CD
cord-299281-5z1xminb	173	19	min	min	NN
cord-299281-5z1xminb	173	20	.	.	.
cord-299281-5z1xminb	174	1	The	the	DT
cord-299281-5z1xminb	174	2	kinetics	kinetic	NNS
cord-299281-5z1xminb	174	3	of	of	IN
cord-299281-5z1xminb	174	4	Gml	Gml	NNP
cord-299281-5z1xminb	174	5	oligomer	oligomer	NNP
cord-299281-5z1xminb	174	6	formation	formation	NN
cord-299281-5z1xminb	174	7	were	be	VBD
cord-299281-5z1xminb	174	8	consistent	consistent	JJ
cord-299281-5z1xminb	174	9	with	with	IN
cord-299281-5z1xminb	174	10	the	the	DT
cord-299281-5z1xminb	174	11	time	time	NN
cord-299281-5z1xminb	174	12	at	at	IN
cord-299281-5z1xminb	174	13	which	which	WDT
cord-299281-5z1xminb	174	14	newly	newly	RB
cord-299281-5z1xminb	174	15	synthesized	synthesize	VBN
cord-299281-5z1xminb	174	16	proteins	protein	NNS
cord-299281-5z1xminb	174	17	arrive	arrive	VBP
cord-299281-5z1xminb	174	18	at	at	IN
cord-299281-5z1xminb	174	19	the	the	DT
cord-299281-5z1xminb	174	20	cis	cis	NNP
cord-299281-5z1xminb	174	21	-	-	HYPH
cord-299281-5z1xminb	174	22	Golgi	Golgi	NNP
cord-299281-5z1xminb	174	23	.	.	.
cord-299281-5z1xminb	175	1	To	to	TO
cord-299281-5z1xminb	175	2	determine	determine	VB
cord-299281-5z1xminb	175	3	if	if	IN
cord-299281-5z1xminb	175	4	oligomerization	oligomerization	NN
cord-299281-5z1xminb	175	5	occurred	occur	VBD
cord-299281-5z1xminb	175	6	in	in	IN
cord-299281-5z1xminb	175	7	a	a	DT
cord-299281-5z1xminb	175	8	post	post	JJ
cord-299281-5z1xminb	175	9	-	-	JJ
cord-299281-5z1xminb	175	10	ER	er	JJ
cord-299281-5z1xminb	175	11	compartment	compartment	NN
cord-299281-5z1xminb	175	12	,	,	,
cord-299281-5z1xminb	175	13	we	-PRON-	PRP
cord-299281-5z1xminb	175	14	asked	ask	VBD
cord-299281-5z1xminb	175	15	whether	whether	IN
cord-299281-5z1xminb	175	16	the	the	DT
cord-299281-5z1xminb	175	17	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	175	18	oligomer	oligomer	NN
cord-299281-5z1xminb	175	19	would	would	MD
cord-299281-5z1xminb	175	20	form	form	VB
cord-299281-5z1xminb	175	21	when	when	WRB
cord-299281-5z1xminb	175	22	ER	ER	NNP
cord-299281-5z1xminb	175	23	to	to	IN
cord-299281-5z1xminb	175	24	Golgi	Golgi	NNP
cord-299281-5z1xminb	175	25	traffic	traffic	NN
cord-299281-5z1xminb	175	26	was	be	VBD
cord-299281-5z1xminb	175	27	blocked	block	VBN
cord-299281-5z1xminb	175	28	by	by	IN
cord-299281-5z1xminb	175	29	incubation	incubation	NN
cord-299281-5z1xminb	175	30	at	at	IN
cord-299281-5z1xminb	175	31	16	16	CD
cord-299281-5z1xminb	175	32	°	°	NN
cord-299281-5z1xminb	175	33	C	c	NN
cord-299281-5z1xminb	175	34	.	.	.
cord-299281-5z1xminb	176	1	At	at	IN
cord-299281-5z1xminb	176	2	this	this	DT
cord-299281-5z1xminb	176	3	temperature	temperature	NN
cord-299281-5z1xminb	176	4	,	,	,
cord-299281-5z1xminb	176	5	newly	newly	RB
cord-299281-5z1xminb	176	6	synthesized	synthesize	VBN
cord-299281-5z1xminb	176	7	proteins	protein	NNS
cord-299281-5z1xminb	176	8	accumulate	accumulate	VBP
cord-299281-5z1xminb	176	9	in	in	IN
cord-299281-5z1xminb	176	10	the	the	DT
cord-299281-5z1xminb	176	11	intermediate	intermediate	JJ
cord-299281-5z1xminb	176	12	compartment	compartment	NN
cord-299281-5z1xminb	176	13	(	(	-LRB-
cord-299281-5z1xminb	176	14	Saraste	Saraste	NNP
cord-299281-5z1xminb	176	15	and	and	CC
cord-299281-5z1xminb	176	16	Kuismanen	Kuismanen	NNP
cord-299281-5z1xminb	176	17	,	,	,
cord-299281-5z1xminb	176	18	1984	1984	CD
cord-299281-5z1xminb	176	19	)	)	-RRB-
cord-299281-5z1xminb	176	20	.	.	.
cord-299281-5z1xminb	177	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	177	2	cells	cell	NNS
cord-299281-5z1xminb	177	3	were	be	VBD
cord-299281-5z1xminb	177	4	transfected	transfecte	VBN
cord-299281-5z1xminb	177	5	with	with	IN
cord-299281-5z1xminb	177	6	constructs	construct	NNS
cord-299281-5z1xminb	177	7	encoding	encode	VBG
cord-299281-5z1xminb	177	8	Gml	Gml	NNP
cord-299281-5z1xminb	177	9	or	or	CC
cord-299281-5z1xminb	177	10	VSV	VSV	NNP
cord-299281-5z1xminb	177	11	G	G	NNP
cord-299281-5z1xminb	177	12	,	,	,
cord-299281-5z1xminb	177	13	metabolically	metabolically	RB
cord-299281-5z1xminb	177	14	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	177	15	for	for	IN
cord-299281-5z1xminb	177	16	5	5	CD
cord-299281-5z1xminb	177	17	min	min	NN
cord-299281-5z1xminb	177	18	,	,	,
cord-299281-5z1xminb	177	19	and	and	CC
cord-299281-5z1xminb	177	20	then	then	RB
cord-299281-5z1xminb	177	21	chased	chase	VBD
cord-299281-5z1xminb	177	22	at	at	IN
cord-299281-5z1xminb	177	23	16	16	CD
cord-299281-5z1xminb	177	24	or	or	CC
cord-299281-5z1xminb	177	25	37	37	CD
cord-299281-5z1xminb	177	26	°	°	NN
cord-299281-5z1xminb	177	27	C	c	NN
cord-299281-5z1xminb	177	28	as	as	IN
cord-299281-5z1xminb	177	29	indicated	indicate	VBN
cord-299281-5z1xminb	177	30	for	for	IN
cord-299281-5z1xminb	177	31	up	up	IN
cord-299281-5z1xminb	177	32	to	to	TO
cord-299281-5z1xminb	177	33	90	90	CD
cord-299281-5z1xminb	177	34	min	min	NN
cord-299281-5z1xminb	177	35	(	(	-LRB-
cord-299281-5z1xminb	177	36	Fig	Fig	NNP
cord-299281-5z1xminb	177	37	.	.	NNP
cord-299281-5z1xminb	177	38	4	4	CD
cord-299281-5z1xminb	177	39	)	)	-RRB-
cord-299281-5z1xminb	177	40	.	.	.
cord-299281-5z1xminb	178	1	No	no	DT
cord-299281-5z1xminb	178	2	oligomer	oligomer	NNP
cord-299281-5z1xminb	178	3	formed	form	VBD
cord-299281-5z1xminb	178	4	when	when	WRB
cord-299281-5z1xminb	178	5	cells	cell	NNS
cord-299281-5z1xminb	178	6	expressing	express	VBG
cord-299281-5z1xminb	178	7	Gml	Gml	NNP
cord-299281-5z1xminb	178	8	were	be	VBD
cord-299281-5z1xminb	178	9	chased	chase	VBN
cord-299281-5z1xminb	178	10	at	at	IN
cord-299281-5z1xminb	178	11	160C	160c	CD
cord-299281-5z1xminb	178	12	;	;	:
cord-299281-5z1xminb	178	13	however	however	RB
cord-299281-5z1xminb	178	14	,	,	,
cord-299281-5z1xminb	178	15	if	if	IN
cord-299281-5z1xminb	178	16	cells	cell	NNS
cord-299281-5z1xminb	178	17	chased	chase	VBN
cord-299281-5z1xminb	178	18	at	at	IN
cord-299281-5z1xminb	178	19	16	16	CD
cord-299281-5z1xminb	178	20	°	°	NN
cord-299281-5z1xminb	178	21	C	C	NNP
cord-299281-5z1xminb	178	22	were	be	VBD
cord-299281-5z1xminb	178	23	subsequently	subsequently	RB
cord-299281-5z1xminb	178	24	transferred	transfer	VBN
cord-299281-5z1xminb	178	25	to	to	IN
cord-299281-5z1xminb	178	26	370C	370C	NNP
cord-299281-5z1xminb	178	27	,	,	,
cord-299281-5z1xminb	178	28	oligomer	oligomer	NNP
cord-299281-5z1xminb	178	29	formation	formation	NN
cord-299281-5z1xminb	178	30	occurred	occur	VBD
cord-299281-5z1xminb	178	31	.	.	.
cord-299281-5z1xminb	179	1	In	in	IN
cord-299281-5z1xminb	179	2	separate	separate	JJ
cord-299281-5z1xminb	179	3	experiments	experiment	NNS
cord-299281-5z1xminb	179	4	,	,	,
cord-299281-5z1xminb	179	5	we	-PRON-	PRP
cord-299281-5z1xminb	179	6	found	find	VBD
cord-299281-5z1xminb	179	7	that	that	IN
cord-299281-5z1xminb	179	8	the	the	DT
cord-299281-5z1xminb	179	9	kinetics	kinetics	NNP
cord-299281-5z1xminb	179	10	of	of	IN
cord-299281-5z1xminb	179	11	oligomer	oligomer	NNP
cord-299281-5z1xminb	179	12	formation	formation	NN
cord-299281-5z1xminb	179	13	at	at	IN
cord-299281-5z1xminb	179	14	37	37	CD
cord-299281-5z1xminb	179	15	°	°	NN
cord-299281-5z1xminb	179	16	C	C	NNP
cord-299281-5z1xminb	179	17	were	be	VBD
cord-299281-5z1xminb	179	18	unaffected	unaffected	JJ
cord-299281-5z1xminb	179	19	by	by	IN
cord-299281-5z1xminb	179	20	the	the	DT
cord-299281-5z1xminb	179	21	inclusion	inclusion	NN
cord-299281-5z1xminb	179	22	of	of	IN
cord-299281-5z1xminb	179	23	a	a	DT
cord-299281-5z1xminb	179	24	160C	160c	CD
cord-299281-5z1xminb	179	25	pretreatment	pretreatment	NN
cord-299281-5z1xminb	179	26	step	step	NN
cord-299281-5z1xminb	179	27	(	(	-LRB-
cord-299281-5z1xminb	179	28	data	datum	NNS
cord-299281-5z1xminb	179	29	not	not	RB
cord-299281-5z1xminb	179	30	shown	show	VBN
cord-299281-5z1xminb	179	31	)	)	-RRB-
cord-299281-5z1xminb	179	32	.	.	.
cord-299281-5z1xminb	180	1	In	in	IN
cord-299281-5z1xminb	180	2	addition	addition	NN
cord-299281-5z1xminb	180	3	,	,	,
cord-299281-5z1xminb	180	4	treatment	treatment	NN
cord-299281-5z1xminb	180	5	with	with	IN
cord-299281-5z1xminb	180	6	0.3	0.3	CD
cord-299281-5z1xminb	180	7	mM	mm	CD
cord-299281-5z1xminb	180	8	CCCP	CCCP	NNP
cord-299281-5z1xminb	180	9	during	during	IN
cord-299281-5z1xminb	180	10	the	the	DT
cord-299281-5z1xminb	180	11	chase	chase	NN
cord-299281-5z1xminb	180	12	period	period	NN
cord-299281-5z1xminb	180	13	also	also	RB
cord-299281-5z1xminb	180	14	inhibited	inhibit	VBD
cord-299281-5z1xminb	180	15	SDS	SDS	NNP
cord-299281-5z1xminb	180	16	-	-	HYPH
cord-299281-5z1xminb	180	17	resistant	resistant	JJ
cord-299281-5z1xminb	180	18	oligomer	oligomer	NNP
cord-299281-5z1xminb	180	19	formation	formation	NN
cord-299281-5z1xminb	180	20	(	(	-LRB-
cord-299281-5z1xminb	180	21	not	not	RB
cord-299281-5z1xminb	180	22	shown	show	VBN
cord-299281-5z1xminb	180	23	)	)	-RRB-
cord-299281-5z1xminb	180	24	.	.	.
cord-299281-5z1xminb	181	1	This	this	DT
cord-299281-5z1xminb	181	2	treatment	treatment	NN
cord-299281-5z1xminb	181	3	blocks	block	VBZ
cord-299281-5z1xminb	181	4	transport	transport	NN
cord-299281-5z1xminb	181	5	of	of	IN
cord-299281-5z1xminb	181	6	newly	newly	RB
cord-299281-5z1xminb	181	7	synthesized	synthesize	VBN
cord-299281-5z1xminb	181	8	proteins	protein	NNS
cord-299281-5z1xminb	181	9	from	from	IN
cord-299281-5z1xminb	181	10	the	the	DT
cord-299281-5z1xminb	181	11	ER	ER	NNP
cord-299281-5z1xminb	181	12	.	.	.
cord-299281-5z1xminb	182	1	These	these	DT
cord-299281-5z1xminb	182	2	experiments	experiment	NNS
cord-299281-5z1xminb	182	3	suggested	suggest	VBD
cord-299281-5z1xminb	182	4	that	that	IN
cord-299281-5z1xminb	182	5	Gml	Gml	NNP
cord-299281-5z1xminb	182	6	oligomer	oligomer	NNP
cord-299281-5z1xminb	182	7	formation	formation	NN
cord-299281-5z1xminb	182	8	normally	normally	RB
cord-299281-5z1xminb	182	9	occurred	occur	VBD
cord-299281-5z1xminb	182	10	in	in	IN
cord-299281-5z1xminb	182	11	a	a	DT
cord-299281-5z1xminb	182	12	post	post	JJ
cord-299281-5z1xminb	182	13	-	-	JJ
cord-299281-5z1xminb	182	14	ER	er	JJ
cord-299281-5z1xminb	182	15	compartment	compartment	NN
cord-299281-5z1xminb	182	16	.	.	.
cord-299281-5z1xminb	183	1	However	however	RB
cord-299281-5z1xminb	183	2	,	,	,
cord-299281-5z1xminb	183	3	brefeldin	brefeldin	NNP
cord-299281-5z1xminb	183	4	A	a	DT
cord-299281-5z1xminb	183	5	pretreatment	pretreatment	NN
cord-299281-5z1xminb	183	6	(	(	-LRB-
cord-299281-5z1xminb	183	7	or	or	CC
cord-299281-5z1xminb	183	8	treatment	treatment	NN
cord-299281-5z1xminb	183	9	during	during	IN
cord-299281-5z1xminb	183	10	chase	chase	NNP
cord-299281-5z1xminb	183	11	)	)	-RRB-
cord-299281-5z1xminb	183	12	did	do	VBD
cord-299281-5z1xminb	183	13	not	not	RB
cord-299281-5z1xminb	183	14	block	block	VB
cord-299281-5z1xminb	183	15	oligomer	oligomer	NNP
cord-299281-5z1xminb	183	16	formation	formation	NN
cord-299281-5z1xminb	183	17	(	(	-LRB-
cord-299281-5z1xminb	183	18	not	not	RB
cord-299281-5z1xminb	183	19	shown	show	VBN
cord-299281-5z1xminb	183	20	)	)	-RRB-
cord-299281-5z1xminb	183	21	,	,	,
cord-299281-5z1xminb	183	22	although	although	IN
cord-299281-5z1xminb	183	23	it	-PRON-	PRP
cord-299281-5z1xminb	183	24	did	do	VBD
cord-299281-5z1xminb	183	25	cause	cause	VB
cord-299281-5z1xminb	183	26	redistribution	redistribution	NN
cord-299281-5z1xminb	183	27	of	of	IN
cord-299281-5z1xminb	183	28	Gml	Gml	NNP
cord-299281-5z1xminb	183	29	to	to	IN
cord-299281-5z1xminb	183	30	an	an	DT
cord-299281-5z1xminb	183	31	ER	er	NN
cord-299281-5z1xminb	183	32	-	-	HYPH
cord-299281-5z1xminb	183	33	like	like	JJ
cord-299281-5z1xminb	183	34	pattern	pattern	NN
cord-299281-5z1xminb	184	1	(	(	-LRB-
cord-299281-5z1xminb	184	2	Machamer	Machamer	NNP
cord-299281-5z1xminb	184	3	et	et	NNP
cord-299281-5z1xminb	184	4	al	al	NNP
cord-299281-5z1xminb	184	5	.	.	NNP
cord-299281-5z1xminb	184	6	,	,	,
cord-299281-5z1xminb	184	7	1993	1993	CD
cord-299281-5z1xminb	184	8	)	)	-RRB-
cord-299281-5z1xminb	184	9	.	.	.
cord-299281-5z1xminb	185	1	To	to	TO
cord-299281-5z1xminb	185	2	ask	ask	VB
cord-299281-5z1xminb	185	3	if	if	IN
cord-299281-5z1xminb	185	4	Gml	Gml	NNP
cord-299281-5z1xminb	185	5	oligomerization	oligomerization	NN
cord-299281-5z1xminb	185	6	was	be	VBD
cord-299281-5z1xminb	185	7	an	an	DT
cord-299281-5z1xminb	185	8	artifact	artifact	NN
cord-299281-5z1xminb	185	9	generated	generate	VBN
cord-299281-5z1xminb	185	10	during	during	IN
cord-299281-5z1xminb	185	11	SDS	SDS	NNP
cord-299281-5z1xminb	185	12	-	-	HYPH
cord-299281-5z1xminb	185	13	PAGE	PAGE	NNP
cord-299281-5z1xminb	185	14	,	,	,
cord-299281-5z1xminb	185	15	we	-PRON-	PRP
cord-299281-5z1xminb	185	16	analyzed	analyze	VBD
cord-299281-5z1xminb	185	17	oligomer	oligomer	NNP
cord-299281-5z1xminb	185	18	formation	formation	NN
cord-299281-5z1xminb	185	19	using	use	VBG
cord-299281-5z1xminb	185	20	velocity	velocity	NN
cord-299281-5z1xminb	185	21	gradient	gradient	NN
cord-299281-5z1xminb	185	22	sedimentation	sedimentation	NN
cord-299281-5z1xminb	185	23	in	in	IN
cord-299281-5z1xminb	185	24	sucrose	sucrose	NN
cord-299281-5z1xminb	185	25	(	(	-LRB-
cord-299281-5z1xminb	185	26	Doms	Doms	NNP
cord-299281-5z1xminb	185	27	et	et	FW
cord-299281-5z1xminb	185	28	al	al	NNP
cord-299281-5z1xminb	185	29	.	.	NNP
cord-299281-5z1xminb	185	30	,	,	,
cord-299281-5z1xminb	185	31	198	198	CD
cord-299281-5z1xminb	185	32	%	%	NN
cord-299281-5z1xminb	185	33	Gml	Gml	NNP
cord-299281-5z1xminb	185	34	does	do	VBZ
cord-299281-5z1xminb	185	35	not	not	RB
cord-299281-5z1xminb	185	36	occur	occur	VB
cord-299281-5z1xminb	185	37	at	at	IN
cord-299281-5z1xminb	185	38	16	16	CD
cord-299281-5z1xminb	185	39	°	°	NN
cord-299281-5z1xminb	185	40	C	c	NN
cord-299281-5z1xminb	185	41	.	.	.
cord-299281-5z1xminb	186	1	Transfected	Transfected	NNP
cord-299281-5z1xminb	186	2	HeLa	HeLa	NNP
cord-299281-5z1xminb	186	3	cells	cell	NNS
cord-299281-5z1xminb	186	4	were	be	VBD
cord-299281-5z1xminb	186	5	metabolically	metabolically	RB
cord-299281-5z1xminb	186	6	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	186	7	for	for	IN
cord-299281-5z1xminb	186	8	5	5	CD
cord-299281-5z1xminb	186	9	min	min	NN
cord-299281-5z1xminb	186	10	,	,	,
cord-299281-5z1xminb	186	11	and	and	CC
cord-299281-5z1xminb	186	12	then	then	RB
cord-299281-5z1xminb	186	13	chased	chase	VBD
cord-299281-5z1xminb	186	14	at	at	IN
cord-299281-5z1xminb	186	15	16	16	CD
cord-299281-5z1xminb	186	16	or	or	CC
cord-299281-5z1xminb	186	17	37	37	CD
cord-299281-5z1xminb	186	18	°	°	NN
cord-299281-5z1xminb	186	19	C	c	NN
cord-299281-5z1xminb	186	20	for	for	IN
cord-299281-5z1xminb	186	21	up	up	IN
cord-299281-5z1xminb	186	22	to	to	TO
cord-299281-5z1xminb	186	23	90	90	CD
cord-299281-5z1xminb	186	24	rain	rain	NN
cord-299281-5z1xminb	186	25	.	.	.
cord-299281-5z1xminb	187	1	One	one	CD
cord-299281-5z1xminb	187	2	set	set	NN
cord-299281-5z1xminb	187	3	of	of	IN
cord-299281-5z1xminb	187	4	dishes	dish	NNS
cord-299281-5z1xminb	187	5	was	be	VBD
cord-299281-5z1xminb	187	6	incubated	incubate	VBN
cord-299281-5z1xminb	187	7	for	for	IN
cord-299281-5z1xminb	187	8	90	90	CD
cord-299281-5z1xminb	187	9	min	min	NN
cord-299281-5z1xminb	187	10	at	at	IN
cord-299281-5z1xminb	187	11	16	16	CD
cord-299281-5z1xminb	187	12	°	°	NN
cord-299281-5z1xminb	187	13	C	c	NN
cord-299281-5z1xminb	187	14	,	,	,
cord-299281-5z1xminb	187	15	then	then	RB
cord-299281-5z1xminb	187	16	transferred	transfer	VBD
cord-299281-5z1xminb	187	17	to	to	IN
cord-299281-5z1xminb	187	18	37	37	CD
cord-299281-5z1xminb	187	19	°	°	NN
cord-299281-5z1xminb	187	20	C	c	NN
cord-299281-5z1xminb	187	21	for	for	IN
cord-299281-5z1xminb	187	22	60	60	CD
cord-299281-5z1xminb	187	23	min	min	NN
cord-299281-5z1xminb	187	24	.	.	.
cord-299281-5z1xminb	188	1	VSV	VSV	NNP
cord-299281-5z1xminb	188	2	G	G	NNP
cord-299281-5z1xminb	188	3	and	and	CC
cord-299281-5z1xminb	188	4	related	relate	VBN
cord-299281-5z1xminb	188	5	proteins	protein	NNS
cord-299281-5z1xminb	188	6	were	be	VBD
cord-299281-5z1xminb	188	7	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	188	8	from	from	IN
cord-299281-5z1xminb	188	9	solubilized	solubilized	JJ
cord-299281-5z1xminb	188	10	cells	cell	NNS
cord-299281-5z1xminb	188	11	as	as	IN
cord-299281-5z1xminb	188	12	described	describe	VBN
cord-299281-5z1xminb	188	13	in	in	IN
cord-299281-5z1xminb	188	14	Materials	material	NNS
cord-299281-5z1xminb	188	15	and	and	CC
cord-299281-5z1xminb	188	16	Methods	method	NNS
cord-299281-5z1xminb	188	17	.	.	.
cord-299281-5z1xminb	189	1	1988	1988	CD
cord-299281-5z1xminb	189	2	)	)	-RRB-
cord-299281-5z1xminb	189	3	.	.	.
cord-299281-5z1xminb	190	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	190	2	cells	cell	NNS
cord-299281-5z1xminb	190	3	were	be	VBD
cord-299281-5z1xminb	190	4	transiently	transiently	RB
cord-299281-5z1xminb	190	5	transfected	transfecte	VBN
cord-299281-5z1xminb	190	6	with	with	IN
cord-299281-5z1xminb	190	7	VSV	VSV	NNP
cord-299281-5z1xminb	190	8	G	G	NNP
cord-299281-5z1xminb	190	9	or	or	CC
cord-299281-5z1xminb	190	10	Gml	Gml	NNP
cord-299281-5z1xminb	190	11	,	,	,
cord-299281-5z1xminb	190	12	and	and	CC
cord-299281-5z1xminb	190	13	then	then	RB
cord-299281-5z1xminb	190	14	metabolically	metabolically	RB
cord-299281-5z1xminb	190	15	radiolabeled	radiolabele	VBD
cord-299281-5z1xminb	190	16	for	for	IN
cord-299281-5z1xminb	190	17	5	5	CD
cord-299281-5z1xminb	190	18	min	min	NN
cord-299281-5z1xminb	190	19	and	and	CC
cord-299281-5z1xminb	190	20	solubilized	solubilize	VBN
cord-299281-5z1xminb	190	21	either	either	CC
cord-299281-5z1xminb	190	22	immediately	immediately	RB
cord-299281-5z1xminb	190	23	or	or	CC
cord-299281-5z1xminb	190	24	after	after	IN
cord-299281-5z1xminb	190	25	60	60	CD
cord-299281-5z1xminb	190	26	min	min	NN
cord-299281-5z1xminb	190	27	of	of	IN
cord-299281-5z1xminb	190	28	chase	chase	NN
cord-299281-5z1xminb	190	29	.	.	.
cord-299281-5z1xminb	191	1	Lysates	Lysates	NNP
cord-299281-5z1xminb	191	2	were	be	VBD
cord-299281-5z1xminb	191	3	loaded	load	VBN
cord-299281-5z1xminb	191	4	onto	onto	IN
cord-299281-5z1xminb	191	5	5	5	CD
cord-299281-5z1xminb	191	6	-	-	SYM
cord-299281-5z1xminb	191	7	20	20	CD
cord-299281-5z1xminb	191	8	%	%	NN
cord-299281-5z1xminb	191	9	sucrose	sucrose	NN
cord-299281-5z1xminb	191	10	gradients	gradient	NNS
cord-299281-5z1xminb	191	11	,	,	,
cord-299281-5z1xminb	191	12	centrifuged	centrifuge	VBN
cord-299281-5z1xminb	191	13	,	,	,
cord-299281-5z1xminb	191	14	and	and	CC
cord-299281-5z1xminb	191	15	analyzed	analyze	VBD
cord-299281-5z1xminb	191	16	as	as	IN
cord-299281-5z1xminb	191	17	described	describe	VBN
cord-299281-5z1xminb	191	18	in	in	IN
cord-299281-5z1xminb	191	19	Materials	material	NNS
cord-299281-5z1xminb	191	20	and	and	CC
cord-299281-5z1xminb	191	21	Methods	method	NNS
cord-299281-5z1xminb	191	22	.	.	.
cord-299281-5z1xminb	192	1	Immediately	immediately	RB
cord-299281-5z1xminb	192	2	after	after	IN
cord-299281-5z1xminb	192	3	synthesis	synthesis	NN
cord-299281-5z1xminb	192	4	,	,	,
cord-299281-5z1xminb	192	5	VSV	VSV	NNP
cord-299281-5z1xminb	192	6	G	G	NNP
cord-299281-5z1xminb	192	7	migrated	migrate	VBD
cord-299281-5z1xminb	192	8	as	as	IN
cord-299281-5z1xminb	192	9	a	a	DT
cord-299281-5z1xminb	192	10	4S20.~	4s20.~	CD
cord-299281-5z1xminb	192	11	peak	peak	NN
cord-299281-5z1xminb	192	12	(	(	-LRB-
cord-299281-5z1xminb	192	13	Fig	Fig	NNP
cord-299281-5z1xminb	192	14	.	.	NNP
cord-299281-5z1xminb	192	15	5	5	CD
cord-299281-5z1xminb	192	16	;	;	:
cord-299281-5z1xminb	192	17	Doms	Doms	NNPS
cord-299281-5z1xminb	192	18	et	et	FW
cord-299281-5z1xminb	192	19	al	al	NNP
cord-299281-5z1xminb	192	20	.	.	NNP
cord-299281-5z1xminb	192	21	,	,	,
cord-299281-5z1xminb	192	22	1987	1987	CD
cord-299281-5z1xminb	192	23	)	)	-RRB-
cord-299281-5z1xminb	192	24	.	.	.
cord-299281-5z1xminb	193	1	After	after	IN
cord-299281-5z1xminb	193	2	a	a	DT
cord-299281-5z1xminb	193	3	60-min	60-min	CD
cord-299281-5z1xminb	193	4	chase	chase	NN
cord-299281-5z1xminb	193	5	,	,	,
cord-299281-5z1xminb	193	6	all	all	DT
cord-299281-5z1xminb	193	7	of	of	IN
cord-299281-5z1xminb	193	8	the	the	DT
cord-299281-5z1xminb	193	9	VSV	VSV	NNP
cord-299281-5z1xminb	193	10	G	G	NNP
cord-299281-5z1xminb	193	11	had	have	VBD
cord-299281-5z1xminb	193	12	trimerized	trimerize	VBN
cord-299281-5z1xminb	193	13	,	,	,
cord-299281-5z1xminb	193	14	migrating	migrate	VBG
cord-299281-5z1xminb	193	15	as	as	IN
cord-299281-5z1xminb	193	16	a	a	DT
cord-299281-5z1xminb	193	17	discrete	discrete	JJ
cord-299281-5z1xminb	193	18	8S20,~	8s20,~	CD
cord-299281-5z1xminb	193	19	peak	peak	NN
cord-299281-5z1xminb	193	20	.	.	.
cord-299281-5z1xminb	194	1	The	the	DT
cord-299281-5z1xminb	194	2	behavior	behavior	NN
cord-299281-5z1xminb	194	3	of	of	IN
cord-299281-5z1xminb	194	4	Gml	Gml	NNP
cord-299281-5z1xminb	194	5	in	in	IN
cord-299281-5z1xminb	194	6	these	these	DT
cord-299281-5z1xminb	194	7	gradients	gradient	NNS
cord-299281-5z1xminb	194	8	was	be	VBD
cord-299281-5z1xminb	194	9	markedly	markedly	RB
cord-299281-5z1xminb	194	10	different	different	JJ
cord-299281-5z1xminb	194	11	.	.	.
cord-299281-5z1xminb	195	1	Immediately	immediately	RB
cord-299281-5z1xminb	195	2	after	after	IN
cord-299281-5z1xminb	195	3	synthesis	synthesis	NN
cord-299281-5z1xminb	195	4	,	,	,
cord-299281-5z1xminb	195	5	Gml	Gml	NNP
cord-299281-5z1xminb	195	6	migrated	migrate	VBD
cord-299281-5z1xminb	195	7	similarly	similarly	RB
cord-299281-5z1xminb	195	8	to	to	IN
cord-299281-5z1xminb	195	9	the	the	DT
cord-299281-5z1xminb	195	10	VSV	VSV	NNP
cord-299281-5z1xminb	195	11	G	G	NNP
cord-299281-5z1xminb	195	12	monomer	monomer	NN
cord-299281-5z1xminb	195	13	.	.	.
cord-299281-5z1xminb	196	1	After	after	IN
cord-299281-5z1xminb	196	2	a	a	DT
cord-299281-5z1xminb	196	3	60-rain	60-rain	CD
cord-299281-5z1xminb	196	4	chase	chase	NN
cord-299281-5z1xminb	196	5	,	,	,
cord-299281-5z1xminb	196	6	however	however	RB
cord-299281-5z1xminb	196	7	,	,	,
cord-299281-5z1xminb	196	8	much	much	JJ
cord-299281-5z1xminb	196	9	of	of	IN
cord-299281-5z1xminb	196	10	the	the	DT
cord-299281-5z1xminb	196	11	Gml	Gml	NNP
cord-299281-5z1xminb	196	12	was	be	VBD
cord-299281-5z1xminb	196	13	found	find	VBN
cord-299281-5z1xminb	196	14	in	in	IN
cord-299281-5z1xminb	196	15	the	the	DT
cord-299281-5z1xminb	196	16	pellet	pellet	NN
cord-299281-5z1xminb	196	17	in	in	IN
cord-299281-5z1xminb	196	18	an	an	DT
cord-299281-5z1xminb	196	19	SDS	sds	NN
cord-299281-5z1xminb	196	20	-	-	HYPH
cord-299281-5z1xminb	196	21	resistant	resistant	JJ
cord-299281-5z1xminb	196	22	form	form	NN
cord-299281-5z1xminb	196	23	(	(	-LRB-
cord-299281-5z1xminb	196	24	Fig	fig	NN
cord-299281-5z1xminb	196	25	.	.	NNP
cord-299281-5z1xminb	196	26	5	5	CD
cord-299281-5z1xminb	196	27	;	;	:
cord-299281-5z1xminb	196	28	note	note	VB
cord-299281-5z1xminb	196	29	top	top	NN
cord-299281-5z1xminb	196	30	of	of	IN
cord-299281-5z1xminb	196	31	lane	lane	NN
cord-299281-5z1xminb	196	32	)	)	-RRB-
cord-299281-5z1xminb	196	33	.	.	.
cord-299281-5z1xminb	197	1	The	the	DT
cord-299281-5z1xminb	197	2	timedependent	timedependent	NN
cord-299281-5z1xminb	197	3	shift	shift	NN
cord-299281-5z1xminb	197	4	in	in	IN
cord-299281-5z1xminb	197	5	the	the	DT
cord-299281-5z1xminb	197	6	mobility	mobility	NN
cord-299281-5z1xminb	197	7	of	of	IN
cord-299281-5z1xminb	197	8	the	the	DT
cord-299281-5z1xminb	197	9	oligomer	oligomer	NNP
cord-299281-5z1xminb	197	10	in	in	IN
cord-299281-5z1xminb	198	1	sucrose	sucrose	NN
cord-299281-5z1xminb	198	2	gradients	gradient	NNS
cord-299281-5z1xminb	198	3	suggests	suggest	VBZ
cord-299281-5z1xminb	198	4	that	that	IN
cord-299281-5z1xminb	198	5	oligomers	oligomer	NNS
cord-299281-5z1xminb	198	6	already	already	RB
cord-299281-5z1xminb	198	7	exist	exist	VBP
cord-299281-5z1xminb	198	8	in	in	IN
cord-299281-5z1xminb	198	9	the	the	DT
cord-299281-5z1xminb	198	10	cells	cell	NNS
cord-299281-5z1xminb	198	11	at	at	IN
cord-299281-5z1xminb	198	12	the	the	DT
cord-299281-5z1xminb	198	13	time	time	NN
cord-299281-5z1xminb	198	14	of	of	IN
cord-299281-5z1xminb	198	15	lysis	lysis	NN
cord-299281-5z1xminb	198	16	and	and	CC
cord-299281-5z1xminb	198	17	immunoprecipitation	immunoprecipitation	NN
cord-299281-5z1xminb	198	18	.	.	.
cord-299281-5z1xminb	199	1	The	the	DT
cord-299281-5z1xminb	199	2	SDSsensitive	sdssensitive	JJ
cord-299281-5z1xminb	199	3	material	material	NN
cord-299281-5z1xminb	199	4	in	in	IN
cord-299281-5z1xminb	199	5	the	the	DT
cord-299281-5z1xminb	199	6	Gml	Gml	NNP
cord-299281-5z1xminb	199	7	pellet	pellet	NN
cord-299281-5z1xminb	199	8	fractions	fraction	NNS
cord-299281-5z1xminb	199	9	may	may	MD
cord-299281-5z1xminb	199	10	represent	represent	VB
cord-299281-5z1xminb	199	11	an	an	DT
cord-299281-5z1xminb	199	12	intermediate	intermediate	JJ
cord-299281-5z1xminb	199	13	form	form	NN
cord-299281-5z1xminb	199	14	of	of	IN
cord-299281-5z1xminb	199	15	oligomer	oligomer	NNP
cord-299281-5z1xminb	199	16	that	that	WDT
cord-299281-5z1xminb	199	17	is	be	VBZ
cord-299281-5z1xminb	199	18	not	not	RB
cord-299281-5z1xminb	199	19	yet	yet	RB
cord-299281-5z1xminb	199	20	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	199	21	.	.	.
cord-299281-5z1xminb	200	1	Alternatively	alternatively	RB
cord-299281-5z1xminb	200	2	,	,	,
cord-299281-5z1xminb	200	3	this	this	DT
cord-299281-5z1xminb	200	4	material	material	NN
cord-299281-5z1xminb	200	5	could	could	MD
cord-299281-5z1xminb	200	6	contain	contain	VB
cord-299281-5z1xminb	200	7	protein	protein	NN
cord-299281-5z1xminb	200	8	released	release	VBN
cord-299281-5z1xminb	200	9	from	from	IN
cord-299281-5z1xminb	200	10	the	the	DT
cord-299281-5z1xminb	200	11	SDS	SDS	NNP
cord-299281-5z1xminb	200	12	-	-	HYPH
cord-299281-5z1xminb	200	13	resistant	resistant	JJ
cord-299281-5z1xminb	200	14	oligomer	oligomer	NN
cord-299281-5z1xminb	200	15	during	during	IN
cord-299281-5z1xminb	200	16	SDS	SDS	NNP
cord-299281-5z1xminb	200	17	-	-	HYPH
cord-299281-5z1xminb	200	18	PAGE	PAGE	NNP
cord-299281-5z1xminb	200	19	.	.	.
cord-299281-5z1xminb	201	1	In	in	IN
cord-299281-5z1xminb	201	2	contrast	contrast	NN
cord-299281-5z1xminb	201	3	to	to	IN
cord-299281-5z1xminb	201	4	our	-PRON-	PRP$
cord-299281-5z1xminb	201	5	findings	finding	NNS
cord-299281-5z1xminb	201	6	with	with	IN
cord-299281-5z1xminb	201	7	Gml	Gml	NNP
cord-299281-5z1xminb	201	8	,	,	,
cord-299281-5z1xminb	201	9	misfolded	misfolded	JJ
cord-299281-5z1xminb	201	10	mutants	mutant	NNS
cord-299281-5z1xminb	201	11	of	of	IN
cord-299281-5z1xminb	201	12	VSV	VSV	NNP
cord-299281-5z1xminb	201	13	G	G	NNP
cord-299281-5z1xminb	201	14	that	that	WDT
cord-299281-5z1xminb	201	15	aggregate	aggregate	NN
cord-299281-5z1xminb	201	16	in	in	IN
cord-299281-5z1xminb	201	17	the	the	DT
cord-299281-5z1xminb	201	18	ER	ER	NNP
cord-299281-5z1xminb	201	19	are	be	VBP
cord-299281-5z1xminb	201	20	found	find	VBN
cord-299281-5z1xminb	201	21	in	in	IN
cord-299281-5z1xminb	201	22	the	the	DT
cord-299281-5z1xminb	201	23	pellet	pellet	NN
cord-299281-5z1xminb	201	24	(	(	-LRB-
cord-299281-5z1xminb	201	25	and	and	CC
cord-299281-5z1xminb	201	26	are	be	VBP
cord-299281-5z1xminb	201	27	SDS	sds	NN
cord-299281-5z1xminb	201	28	-	-	HYPH
cord-299281-5z1xminb	201	29	soluble	soluble	JJ
cord-299281-5z1xminb	201	30	)	)	-RRB-
cord-299281-5z1xminb	201	31	immediately	immediately	RB
cord-299281-5z1xminb	201	32	after	after	IN
cord-299281-5z1xminb	201	33	synthesis	synthesis	NN
cord-299281-5z1xminb	201	34	(	(	-LRB-
cord-299281-5z1xminb	201	35	Doms	Doms	NNP
cord-299281-5z1xminb	201	36	et	et	FW
cord-299281-5z1xminb	201	37	al	al	NNP
cord-299281-5z1xminb	201	38	.	.	NNP
cord-299281-5z1xminb	201	39	,	,	,
cord-299281-5z1xminb	201	40	1988	1988	CD
cord-299281-5z1xminb	201	41	)	)	-RRB-
cord-299281-5z1xminb	201	42	.	.	.
cord-299281-5z1xminb	202	1	Using	use	VBG
cord-299281-5z1xminb	202	2	different	different	JJ
cord-299281-5z1xminb	202	3	centrifugation	centrifugation	NN
cord-299281-5z1xminb	202	4	conditions	condition	NNS
cord-299281-5z1xminb	202	5	,	,	,
cord-299281-5z1xminb	202	6	we	-PRON-	PRP
cord-299281-5z1xminb	202	7	estimated	estimate	VBD
cord-299281-5z1xminb	202	8	the	the	DT
cord-299281-5z1xminb	202	9	average	average	JJ
cord-299281-5z1xminb	202	10	size	size	NN
cord-299281-5z1xminb	202	11	of	of	IN
cord-299281-5z1xminb	202	12	the	the	DT
cord-299281-5z1xminb	202	13	Gml	Gml	NNP
cord-299281-5z1xminb	202	14	oligomer	oligomer	NN
cord-299281-5z1xminb	202	15	to	to	TO
cord-299281-5z1xminb	202	16	be	be	VB
cord-299281-5z1xminb	202	17	approximately	approximately	RB
cord-299281-5z1xminb	202	18	22S20.~	22s20.~	CD
cord-299281-5z1xminb	202	19	,	,	,
cord-299281-5z1xminb	202	20	or	or	CC
cord-299281-5z1xminb	202	21	roughly	roughly	RB
cord-299281-5z1xminb	202	22	800	800	CD
cord-299281-5z1xminb	202	23	kD	kd	XX
cord-299281-5z1xminb	203	1	(	(	-LRB-
cord-299281-5z1xminb	203	2	data	datum	NNS
cord-299281-5z1xminb	203	3	not	not	RB
cord-299281-5z1xminb	203	4	shown	show	VBN
cord-299281-5z1xminb	203	5	)	)	-RRB-
cord-299281-5z1xminb	203	6	.	.	.
cord-299281-5z1xminb	204	1	Therefore	therefore	RB
cord-299281-5z1xminb	204	2	,	,	,
cord-299281-5z1xminb	204	3	if	if	IN
cord-299281-5z1xminb	204	4	Gml	Gml	NNP
cord-299281-5z1xminb	204	5	(	(	-LRB-
cord-299281-5z1xminb	204	6	MW	MW	NNP
cord-299281-5z1xminb	204	7	66	66	CD
cord-299281-5z1xminb	204	8	kD	kD	NNP
cord-299281-5z1xminb	204	9	)	)	-RRB-
cord-299281-5z1xminb	204	10	is	be	VBZ
cord-299281-5z1xminb	204	11	the	the	DT
cord-299281-5z1xminb	204	12	sole	sole	JJ
cord-299281-5z1xminb	204	13	component	component	NN
cord-299281-5z1xminb	204	14	of	of	IN
cord-299281-5z1xminb	204	15	the	the	DT
cord-299281-5z1xminb	204	16	oligomer	oligomer	NNP
cord-299281-5z1xminb	204	17	,	,	,
cord-299281-5z1xminb	204	18	we	-PRON-	PRP
cord-299281-5z1xminb	204	19	estimate	estimate	VBP
cord-299281-5z1xminb	204	20	there	there	EX
cord-299281-5z1xminb	204	21	would	would	MD
cord-299281-5z1xminb	204	22	be	be	VB
cord-299281-5z1xminb	204	23	,	,	,
cord-299281-5z1xminb	204	24	o12	o12	NN
cord-299281-5z1xminb	204	25	molecules	molecule	NNS
cord-299281-5z1xminb	204	26	per	per	IN
cord-299281-5z1xminb	204	27	oligomer	oligomer	NNP
cord-299281-5z1xminb	204	28	.	.	.
cord-299281-5z1xminb	205	1	In	in	IN
cord-299281-5z1xminb	205	2	contrast	contrast	NN
cord-299281-5z1xminb	205	3	to	to	IN
cord-299281-5z1xminb	205	4	the	the	DT
cord-299281-5z1xminb	205	5	chimeric	chimeric	JJ
cord-299281-5z1xminb	205	6	Gml	Gml	NNP
cord-299281-5z1xminb	205	7	protein	protein	NN
cord-299281-5z1xminb	205	8	,	,	,
cord-299281-5z1xminb	205	9	oligomers	oligomer	NNS
cord-299281-5z1xminb	205	10	of	of	IN
cord-299281-5z1xminb	205	11	the	the	DT
cord-299281-5z1xminb	205	12	IBV	IBV	NNP
cord-299281-5z1xminb	205	13	M	M	NNP
cord-299281-5z1xminb	205	14	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	205	15	are	be	VBP
cord-299281-5z1xminb	205	16	not	not	RB
cord-299281-5z1xminb	205	17	detected	detect	VBN
cord-299281-5z1xminb	205	18	when	when	WRB
cord-299281-5z1xminb	205	19	assayed	assay	VBN
cord-299281-5z1xminb	205	20	by	by	IN
cord-299281-5z1xminb	205	21	sucrose	sucrose	NN
cord-299281-5z1xminb	205	22	gradient	gradient	NN
cord-299281-5z1xminb	205	23	sedimentation	sedimentation	NN
cord-299281-5z1xminb	205	24	(	(	-LRB-
cord-299281-5z1xminb	205	25	data	datum	NNS
cord-299281-5z1xminb	205	26	not	not	RB
cord-299281-5z1xminb	205	27	shown	show	VBN
cord-299281-5z1xminb	205	28	)	)	-RRB-
cord-299281-5z1xminb	205	29	.	.	.
cord-299281-5z1xminb	206	1	This	this	DT
cord-299281-5z1xminb	206	2	might	may	MD
cord-299281-5z1xminb	206	3	suggest	suggest	VB
cord-299281-5z1xminb	206	4	that	that	IN
cord-299281-5z1xminb	206	5	oligomerization	oligomerization	NN
cord-299281-5z1xminb	206	6	is	be	VBZ
cord-299281-5z1xminb	206	7	not	not	RB
cord-299281-5z1xminb	206	8	a	a	DT
cord-299281-5z1xminb	206	9	retention	retention	NN
cord-299281-5z1xminb	206	10	mechanism	mechanism	NN
cord-299281-5z1xminb	206	11	for	for	IN
cord-299281-5z1xminb	206	12	native	native	JJ
cord-299281-5z1xminb	206	13	M	M	NNP
cord-299281-5z1xminb	206	14	protein	protein	NN
cord-299281-5z1xminb	206	15	;	;	:
cord-299281-5z1xminb	206	16	alternatively	alternatively	RB
cord-299281-5z1xminb	206	17	,	,	,
cord-299281-5z1xminb	206	18	M	M	NNP
cord-299281-5z1xminb	206	19	oligomers	oligomer	NNS
cord-299281-5z1xminb	206	20	might	may	MD
cord-299281-5z1xminb	206	21	readily	readily	RB
cord-299281-5z1xminb	206	22	dissociate	dissociate	VB
cord-299281-5z1xminb	206	23	in	in	IN
cord-299281-5z1xminb	206	24	detergent	detergent	NN
cord-299281-5z1xminb	206	25	.	.	.
cord-299281-5z1xminb	207	1	The	the	DT
cord-299281-5z1xminb	207	2	resistance	resistance	NN
cord-299281-5z1xminb	207	3	of	of	IN
cord-299281-5z1xminb	207	4	the	the	DT
cord-299281-5z1xminb	207	5	Gml	Gml	NNP
cord-299281-5z1xminb	207	6	oligomer	oligomer	NN
cord-299281-5z1xminb	207	7	to	to	IN
cord-299281-5z1xminb	207	8	solubilization	solubilization	NN
cord-299281-5z1xminb	207	9	suggests	suggest	VBZ
cord-299281-5z1xminb	207	10	a	a	DT
cord-299281-5z1xminb	207	11	structural	structural	JJ
cord-299281-5z1xminb	207	12	feature	feature	NN
cord-299281-5z1xminb	207	13	of	of	IN
cord-299281-5z1xminb	207	14	VSV	VSV	NNP
cord-299281-5z1xminb	207	15	G	G	NNP
cord-299281-5z1xminb	207	16	may	may	MD
cord-299281-5z1xminb	207	17	stabilize	stabilize	VB
cord-299281-5z1xminb	207	18	the	the	DT
cord-299281-5z1xminb	207	19	complex	complex	NN
cord-299281-5z1xminb	207	20	.	.	.
cord-299281-5z1xminb	208	1	We	-PRON-	PRP
cord-299281-5z1xminb	208	2	asked	ask	VBD
cord-299281-5z1xminb	208	3	whether	whether	IN
cord-299281-5z1xminb	208	4	the	the	DT
cord-299281-5z1xminb	208	5	lumenal	lumenal	JJ
cord-299281-5z1xminb	208	6	domain	domain	NN
cord-299281-5z1xminb	208	7	of	of	IN
cord-299281-5z1xminb	208	8	VSV	VSV	NNP
cord-299281-5z1xminb	208	9	G	G	NNP
cord-299281-5z1xminb	208	10	,	,	,
cord-299281-5z1xminb	208	11	which	which	WDT
cord-299281-5z1xminb	208	12	is	be	VBZ
cord-299281-5z1xminb	208	13	sufficient	sufficient	JJ
cord-299281-5z1xminb	208	14	for	for	IN
cord-299281-5z1xminb	208	15	trimerization	trimerization	NN
cord-299281-5z1xminb	208	16	(	(	-LRB-
cord-299281-5z1xminb	208	17	Doms	Doms	NNP
cord-299281-5z1xminb	208	18	et	et	FW
cord-299281-5z1xminb	208	19	al	al	NNP
cord-299281-5z1xminb	208	20	.	.	NNP
cord-299281-5z1xminb	208	21	,	,	,
cord-299281-5z1xminb	208	22	1988	1988	CD
cord-299281-5z1xminb	208	23	)	)	-RRB-
cord-299281-5z1xminb	208	24	,	,	,
cord-299281-5z1xminb	208	25	was	be	VBD
cord-299281-5z1xminb	208	26	required	require	VBN
cord-299281-5z1xminb	208	27	for	for	IN
cord-299281-5z1xminb	208	28	oligomer	oligomer	NNP
cord-299281-5z1xminb	208	29	formation	formation	NN
cord-299281-5z1xminb	208	30	.	.	.
cord-299281-5z1xminb	209	1	To	to	TO
cord-299281-5z1xminb	209	2	do	do	VB
cord-299281-5z1xminb	209	3	this	this	DT
cord-299281-5z1xminb	209	4	,	,	,
cord-299281-5z1xminb	209	5	we	-PRON-	PRP
cord-299281-5z1xminb	209	6	replaced	replace	VBD
cord-299281-5z1xminb	209	7	the	the	DT
cord-299281-5z1xminb	209	8	lumenal	lumenal	JJ
cord-299281-5z1xminb	209	9	domain	domain	NN
cord-299281-5z1xminb	209	10	ofVSV	ofvsv	CD
cord-299281-5z1xminb	209	11	G	g	NN
cord-299281-5z1xminb	209	12	or	or	CC
cord-299281-5z1xminb	209	13	Gml	Gml	NNP
cord-299281-5z1xminb	209	14	with	with	IN
cord-299281-5z1xminb	209	15	a	a	DT
cord-299281-5z1xminb	209	16	small	small	JJ
cord-299281-5z1xminb	209	17	soluble	soluble	JJ
cord-299281-5z1xminb	209	18	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	209	19	,	,	,
cord-299281-5z1xminb	209	20	the	the	DT
cord-299281-5z1xminb	209	21	o~	o~	CD
cord-299281-5z1xminb	209	22	subunit	subunit	NN
cord-299281-5z1xminb	209	23	of	of	IN
cord-299281-5z1xminb	209	24	hCG	hCG	NNP
cord-299281-5z1xminb	209	25	.	.	.
cord-299281-5z1xminb	210	1	When	when	WRB
cord-299281-5z1xminb	210	2	expressed	express	VBN
cord-299281-5z1xminb	210	3	alone	alone	RB
cord-299281-5z1xminb	210	4	,	,	,
cord-299281-5z1xminb	210	5	the	the	DT
cord-299281-5z1xminb	210	6	oL	oL	NNP
cord-299281-5z1xminb	210	7	subunit	subunit	NN
cord-299281-5z1xminb	210	8	of	of	IN
cord-299281-5z1xminb	210	9	hCG	hCG	NNP
cord-299281-5z1xminb	210	10	is	be	VBZ
cord-299281-5z1xminb	210	11	secreted	secrete	VBN
cord-299281-5z1xminb	210	12	from	from	IN
cord-299281-5z1xminb	210	13	cells	cell	NNS
cord-299281-5z1xminb	210	14	as	as	IN
cord-299281-5z1xminb	210	15	a	a	DT
cord-299281-5z1xminb	210	16	monomer	monomer	NN
cord-299281-5z1xminb	210	17	(	(	-LRB-
cord-299281-5z1xminb	210	18	Guan	Guan	NNP
cord-299281-5z1xminb	210	19	et	et	FW
cord-299281-5z1xminb	210	20	al	al	NNP
cord-299281-5z1xminb	210	21	.	.	NNP
cord-299281-5z1xminb	210	22	,	,	,
cord-299281-5z1xminb	210	23	1988b	1988b	CD
cord-299281-5z1xminb	210	24	)	)	-RRB-
cord-299281-5z1xminb	210	25	.	.	.
cord-299281-5z1xminb	211	1	When	when	WRB
cord-299281-5z1xminb	211	2	hCGo~	hCGo~	NNP
cord-299281-5z1xminb	211	3	is	be	VBZ
cord-299281-5z1xminb	211	4	fused	fuse	VBN
cord-299281-5z1xminb	211	5	to	to	IN
cord-299281-5z1xminb	211	6	the	the	DT
cord-299281-5z1xminb	211	7	membrane	membrane	NN
cord-299281-5z1xminb	211	8	-	-	HYPH
cord-299281-5z1xminb	211	9	spanning	span	VBG
cord-299281-5z1xminb	211	10	domain	domain	NN
cord-299281-5z1xminb	211	11	and	and	CC
cord-299281-5z1xminb	211	12	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	211	13	tail	tail	NN
cord-299281-5z1xminb	211	14	of	of	IN
cord-299281-5z1xminb	211	15	VSV	VSV	NNP
cord-299281-5z1xminb	211	16	G	G	NNP
cord-299281-5z1xminb	211	17	,	,	,
cord-299281-5z1xminb	211	18	the	the	DT
cord-299281-5z1xminb	211	19	chimeric	chimeric	JJ
cord-299281-5z1xminb	211	20	protein	protein	NN
cord-299281-5z1xminb	211	21	(	(	-LRB-
cord-299281-5z1xminb	211	22	c	c	NN
cord-299281-5z1xminb	211	23	~	~	NFP
cord-299281-5z1xminb	211	24	m	m	NN
cord-299281-5z1xminb	211	25	)	)	-RRB-
cord-299281-5z1xminb	211	26	is	be	VBZ
cord-299281-5z1xminb	211	27	membrane	membrane	NN
cord-299281-5z1xminb	211	28	-	-	HYPH
cord-299281-5z1xminb	211	29	bound	bind	VBN
cord-299281-5z1xminb	211	30	and	and	CC
cord-299281-5z1xminb	211	31	transported	transport	VBN
cord-299281-5z1xminb	211	32	to	to	IN
cord-299281-5z1xminb	211	33	the	the	DT
cord-299281-5z1xminb	211	34	cell	cell	NN
cord-299281-5z1xminb	211	35	surface	surface	NN
cord-299281-5z1xminb	211	36	(	(	-LRB-
cord-299281-5z1xminb	211	37	Guan	Guan	NNP
cord-299281-5z1xminb	211	38	et	et	FW
cord-299281-5z1xminb	211	39	al	al	NNP
cord-299281-5z1xminb	211	40	.	.	NNP
cord-299281-5z1xminb	211	41	,	,	,
cord-299281-5z1xminb	211	42	1988a	1988a	CD
cord-299281-5z1xminb	211	43	)	)	-RRB-
cord-299281-5z1xminb	211	44	.	.	.
cord-299281-5z1xminb	212	1	When	when	WRB
cord-299281-5z1xminb	212	2	the	the	DT
cord-299281-5z1xminb	212	3	VSV	VSV	NNP
cord-299281-5z1xminb	212	4	G	G	NNP
cord-299281-5z1xminb	212	5	membrane	membrane	NN
cord-299281-5z1xminb	212	6	-	-	HYPH
cord-299281-5z1xminb	212	7	spanning	span	VBG
cord-299281-5z1xminb	212	8	domain	domain	NN
cord-299281-5z1xminb	212	9	of	of	IN
cord-299281-5z1xminb	212	10	c	c	NN
cord-299281-5z1xminb	212	11	~	~	NFP
cord-299281-5z1xminb	212	12	m	m	NNP
cord-299281-5z1xminb	212	13	is	be	VBZ
cord-299281-5z1xminb	212	14	replaced	replace	VBN
cord-299281-5z1xminb	212	15	with	with	IN
cord-299281-5z1xminb	212	16	the	the	DT
cord-299281-5z1xminb	212	17	ml	ml	NN
cord-299281-5z1xminb	212	18	domain	domain	NN
cord-299281-5z1xminb	212	19	of	of	IN
cord-299281-5z1xminb	212	20	IBV	IBV	NNP
cord-299281-5z1xminb	212	21	M	M	NNP
cord-299281-5z1xminb	212	22	,	,	,
cord-299281-5z1xminb	212	23	the	the	DT
cord-299281-5z1xminb	212	24	resulting	result	VBG
cord-299281-5z1xminb	212	25	chimera	chimera	NN
cord-299281-5z1xminb	212	26	(	(	-LRB-
cord-299281-5z1xminb	212	27	oemlG	oemlG	NNP
cord-299281-5z1xminb	212	28	)	)	-RRB-
cord-299281-5z1xminb	212	29	is	be	VBZ
cord-299281-5z1xminb	212	30	retained	retain	VBN
cord-299281-5z1xminb	212	31	in	in	IN
cord-299281-5z1xminb	212	32	the	the	DT
cord-299281-5z1xminb	212	33	Golgi	Golgi	NNP
cord-299281-5z1xminb	212	34	complex	complex	NN
cord-299281-5z1xminb	212	35	(	(	-LRB-
cord-299281-5z1xminb	212	36	Swift	Swift	NNP
cord-299281-5z1xminb	212	37	and	and	CC
cord-299281-5z1xminb	212	38	Machamer	Machamer	NNP
cord-299281-5z1xminb	212	39	,	,	,
cord-299281-5z1xminb	212	40	1991	1991	CD
cord-299281-5z1xminb	212	41	)	)	-RRB-
cord-299281-5z1xminb	212	42	.	.	.
cord-299281-5z1xminb	213	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	213	2	,	,	,
cord-299281-5z1xminb	213	3	in	in	IN
cord-299281-5z1xminb	213	4	metabolically	metabolically	RB
cord-299281-5z1xminb	213	5	labeled	label	VBN
cord-299281-5z1xminb	213	6	HeLa	HeLa	NNP
cord-299281-5z1xminb	213	7	cells	cell	NNS
cord-299281-5z1xminb	213	8	expressing	express	VBG
cord-299281-5z1xminb	213	9	cxmlG	cxmlG	NNP
cord-299281-5z1xminb	213	10	,	,	,
cord-299281-5z1xminb	213	11	we	-PRON-	PRP
cord-299281-5z1xminb	213	12	observed	observe	VBD
cord-299281-5z1xminb	213	13	the	the	DT
cord-299281-5z1xminb	213	14	time	time	NN
cord-299281-5z1xminb	213	15	-	-	HYPH
cord-299281-5z1xminb	213	16	dependent	dependent	JJ
cord-299281-5z1xminb	213	17	accumulation	accumulation	NN
cord-299281-5z1xminb	213	18	of	of	IN
cord-299281-5z1xminb	213	19	an	an	DT
cord-299281-5z1xminb	213	20	SDS	sds	NN
cord-299281-5z1xminb	213	21	-	-	HYPH
cord-299281-5z1xminb	213	22	resistant	resistant	JJ
cord-299281-5z1xminb	213	23	species	specie	NNS
cord-299281-5z1xminb	213	24	at	at	IN
cord-299281-5z1xminb	213	25	the	the	DT
cord-299281-5z1xminb	213	26	interface	interface	NN
cord-299281-5z1xminb	213	27	between	between	IN
cord-299281-5z1xminb	213	28	the	the	DT
cord-299281-5z1xminb	213	29	separating	separate	VBG
cord-299281-5z1xminb	213	30	and	and	CC
cord-299281-5z1xminb	213	31	stacking	stack	VBG
cord-299281-5z1xminb	213	32	gels	gel	NNS
cord-299281-5z1xminb	213	33	(	(	-LRB-
cord-299281-5z1xminb	213	34	Fig	Fig	NNP
cord-299281-5z1xminb	213	35	.	.	NNP
cord-299281-5z1xminb	213	36	6	6	CD
cord-299281-5z1xminb	213	37	,	,	,
cord-299281-5z1xminb	213	38	arrowhead	arrowhead	NNP
cord-299281-5z1xminb	213	39	)	)	-RRB-
cord-299281-5z1xminb	213	40	.	.	.
cord-299281-5z1xminb	214	1	This	this	DT
cord-299281-5z1xminb	214	2	species	species	NN
cord-299281-5z1xminb	214	3	was	be	VBD
cord-299281-5z1xminb	214	4	not	not	RB
cord-299281-5z1xminb	214	5	observed	observe	VBN
cord-299281-5z1xminb	214	6	in	in	IN
cord-299281-5z1xminb	214	7	cells	cell	NNS
cord-299281-5z1xminb	214	8	expressing	express	VBG
cord-299281-5z1xminb	214	9	am	be	VBP
cord-299281-5z1xminb	214	10	.	.	.
cord-299281-5z1xminb	215	1	The	the	DT
cord-299281-5z1xminb	215	2	smaller	small	JJR
cord-299281-5z1xminb	215	3	l	l	NN
cord-299281-5z1xminb	215	4	~	~	NFP
cord-299281-5z1xminb	215	5	gure	gure	NNP
cord-299281-5z1xminb	215	6	5	5	CD
cord-299281-5z1xminb	215	7	.	.	.
cord-299281-5z1xminb	216	1	Sucrose	sucrose	NN
cord-299281-5z1xminb	216	2	gradient	gradient	NN
cord-299281-5z1xminb	216	3	sedimentation	sedimentation	NN
cord-299281-5z1xminb	216	4	of	of	IN
cord-299281-5z1xminb	216	5	Gml	Gml	NNP
cord-299281-5z1xminb	216	6	and	and	CC
cord-299281-5z1xminb	216	7	VSV	VSV	NNP
cord-299281-5z1xminb	216	8	G.	G.	NNP
cord-299281-5z1xminb	216	9	HeLa	HeLa	NNP
cord-299281-5z1xminb	216	10	cells	cell	NNS
cord-299281-5z1xminb	216	11	transfected	transfecte	VBN
cord-299281-5z1xminb	216	12	with	with	IN
cord-299281-5z1xminb	216	13	VSV	VSV	NNP
cord-299281-5z1xminb	216	14	G	G	NNP
cord-299281-5z1xminb	216	15	or	or	CC
cord-299281-5z1xminb	216	16	Gml	Gml	NNP
cord-299281-5z1xminb	216	17	were	be	VBD
cord-299281-5z1xminb	216	18	metabolically	metabolically	RB
cord-299281-5z1xminb	216	19	labeled	label	VBN
cord-299281-5z1xminb	216	20	for	for	IN
cord-299281-5z1xminb	216	21	5	5	CD
cord-299281-5z1xminb	216	22	rain	rain	NN
cord-299281-5z1xminb	216	23	,	,	,
cord-299281-5z1xminb	216	24	and	and	CC
cord-299281-5z1xminb	216	25	then	then	RB
cord-299281-5z1xminb	216	26	solubilized	solubilize	VBD
cord-299281-5z1xminb	216	27	immediately	immediately	RB
cord-299281-5z1xminb	216	28	or	or	CC
cord-299281-5z1xminb	216	29	after	after	IN
cord-299281-5z1xminb	216	30	60	60	CD
cord-299281-5z1xminb	216	31	rain	rain	NN
cord-299281-5z1xminb	216	32	of	of	IN
cord-299281-5z1xminb	216	33	chase	chase	NN
cord-299281-5z1xminb	216	34	.	.	.
cord-299281-5z1xminb	217	1	Lysates	Lysates	NNP
cord-299281-5z1xminb	217	2	were	be	VBD
cord-299281-5z1xminb	217	3	loaded	load	VBN
cord-299281-5z1xminb	217	4	onto	onto	IN
cord-299281-5z1xminb	217	5	5	5	CD
cord-299281-5z1xminb	217	6	-	-	SYM
cord-299281-5z1xminb	217	7	20	20	CD
cord-299281-5z1xminb	217	8	%	%	NN
cord-299281-5z1xminb	217	9	sucrose	sucrose	NN
cord-299281-5z1xminb	217	10	gradients	gradient	NNS
cord-299281-5z1xminb	217	11	and	and	CC
cord-299281-5z1xminb	217	12	centrifuged	centrifuge	VBD
cord-299281-5z1xminb	217	13	as	as	IN
cord-299281-5z1xminb	217	14	described	describe	VBN
cord-299281-5z1xminb	217	15	in	in	IN
cord-299281-5z1xminb	217	16	Materials	material	NNS
cord-299281-5z1xminb	217	17	and	and	CC
cord-299281-5z1xminb	217	18	Methods	method	NNS
cord-299281-5z1xminb	217	19	.	.	.
cord-299281-5z1xminb	218	1	Fractions	fraction	NNS
cord-299281-5z1xminb	218	2	were	be	VBD
cord-299281-5z1xminb	218	3	collected	collect	VBN
cord-299281-5z1xminb	218	4	,	,	,
cord-299281-5z1xminb	218	5	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	218	6	using	use	VBG
cord-299281-5z1xminb	218	7	anti	anti	JJ
cord-299281-5z1xminb	218	8	-	-	JJ
cord-299281-5z1xminb	218	9	VSV	VSV	NNP
cord-299281-5z1xminb	218	10	antibody	antibody	NN
cord-299281-5z1xminb	218	11	,	,	,
cord-299281-5z1xminb	218	12	and	and	CC
cord-299281-5z1xminb	218	13	analyzed	analyze	VBN
cord-299281-5z1xminb	218	14	by	by	IN
cord-299281-5z1xminb	218	15	SDS	SDS	NNP
cord-299281-5z1xminb	218	16	-	-	HYPH
cord-299281-5z1xminb	218	17	PAGE	PAGE	NNP
cord-299281-5z1xminb	218	18	.	.	.
cord-299281-5z1xminb	219	1	After	after	IN
cord-299281-5z1xminb	219	2	the	the	DT
cord-299281-5z1xminb	219	3	60-min	60-min	CD
cord-299281-5z1xminb	219	4	chase	chase	NN
cord-299281-5z1xminb	219	5	,	,	,
cord-299281-5z1xminb	219	6	all	all	DT
cord-299281-5z1xminb	219	7	of	of	IN
cord-299281-5z1xminb	219	8	the	the	DT
cord-299281-5z1xminb	219	9	Gml	Gml	NNP
cord-299281-5z1xminb	219	10	formed	form	VBD
cord-299281-5z1xminb	219	11	a	a	DT
cord-299281-5z1xminb	219	12	large	large	JJ
cord-299281-5z1xminb	219	13	oligomer	oligomer	NN
cord-299281-5z1xminb	219	14	that	that	WDT
cord-299281-5z1xminb	219	15	pelleted	pellete	VBD
cord-299281-5z1xminb	219	16	under	under	IN
cord-299281-5z1xminb	219	17	these	these	DT
cord-299281-5z1xminb	219	18	gradient	gradient	NN
cord-299281-5z1xminb	219	19	conditions	condition	NNS
cord-299281-5z1xminb	219	20	,	,	,
cord-299281-5z1xminb	219	21	and	and	CC
cord-299281-5z1xminb	219	22	much	much	JJ
cord-299281-5z1xminb	219	23	of	of	IN
cord-299281-5z1xminb	219	24	it	-PRON-	PRP
cord-299281-5z1xminb	219	25	was	be	VBD
cord-299281-5z1xminb	219	26	SDS	sds	NN
cord-299281-5z1xminb	219	27	-	-	HYPH
cord-299281-5z1xminb	219	28	resistant	resistant	JJ
cord-299281-5z1xminb	219	29	.	.	.
cord-299281-5z1xminb	220	1	size	size	NN
cord-299281-5z1xminb	220	2	of	of	IN
cord-299281-5z1xminb	220	3	the	the	DT
cord-299281-5z1xminb	220	4	otmlG	otmlG	NNP
cord-299281-5z1xminb	220	5	SDS	SDS	NNP
cord-299281-5z1xminb	220	6	-	-	HYPH
cord-299281-5z1xminb	220	7	resistant	resistant	JJ
cord-299281-5z1xminb	220	8	oligomer	oligomer	NNP
cord-299281-5z1xminb	220	9	is	be	VBZ
cord-299281-5z1xminb	220	10	consistent	consistent	JJ
cord-299281-5z1xminb	220	11	with	with	IN
cord-299281-5z1xminb	220	12	the	the	DT
cord-299281-5z1xminb	220	13	size	size	NN
cord-299281-5z1xminb	220	14	difference	difference	NN
cord-299281-5z1xminb	220	15	between	between	IN
cord-299281-5z1xminb	220	16	ctmlG	ctmlG	NNP
cord-299281-5z1xminb	220	17	and	and	CC
cord-299281-5z1xminb	220	18	Gml	Gml	NNP
cord-299281-5z1xminb	220	19	(	(	-LRB-
cord-299281-5z1xminb	220	20	28	28	CD
cord-299281-5z1xminb	220	21	vs	vs	IN
cord-299281-5z1xminb	220	22	66	66	CD
cord-299281-5z1xminb	220	23	kD	kd	CD
cord-299281-5z1xminb	220	24	)	)	-RRB-
cord-299281-5z1xminb	220	25	.	.	.
cord-299281-5z1xminb	221	1	On	on	IN
cord-299281-5z1xminb	221	2	sucrose	sucrose	JJ
cord-299281-5z1xminb	221	3	gradients	gradient	NNS
cord-299281-5z1xminb	221	4	,	,	,
cord-299281-5z1xminb	221	5	c	c	NNP
cord-299281-5z1xminb	221	6	~	~	NFP
cord-299281-5z1xminb	221	7	mlG	mlG	-LRB-
cord-299281-5z1xminb	221	8	solubilized	solubilize	VBD
cord-299281-5z1xminb	221	9	60	60	CD
cord-299281-5z1xminb	221	10	min	min	NN
cord-299281-5z1xminb	221	11	after	after	IN
cord-299281-5z1xminb	221	12	synthesis	synthesis	NN
cord-299281-5z1xminb	221	13	migrated	migrate	VBD
cord-299281-5z1xminb	221	14	as	as	IN
cord-299281-5z1xminb	221	15	a	a	DT
cord-299281-5z1xminb	221	16	larger	large	JJR
cord-299281-5z1xminb	221	17	species	specie	NNS
cord-299281-5z1xminb	221	18	(	(	-LRB-
cord-299281-5z1xminb	221	19	some	some	DT
cord-299281-5z1xminb	221	20	of	of	IN
cord-299281-5z1xminb	221	21	which	which	WDT
cord-299281-5z1xminb	221	22	was	be	VBD
cord-299281-5z1xminb	221	23	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	221	24	)	)	-RRB-
cord-299281-5z1xminb	221	25	than	than	IN
cord-299281-5z1xminb	221	26	newly	newly	RB
cord-299281-5z1xminb	221	27	synthesized	synthesize	VBN
cord-299281-5z1xminb	221	28	oanlG.	oanlg.	RP
cord-299281-5z1xminb	222	1	In	in	IN
cord-299281-5z1xminb	222	2	contrast	contrast	NN
cord-299281-5z1xminb	222	3	,	,	,
cord-299281-5z1xminb	222	4	the	the	DT
cord-299281-5z1xminb	222	5	mobility	mobility	NN
cord-299281-5z1xminb	222	6	of	of	IN
cord-299281-5z1xminb	222	7	o	o	NN
cord-299281-5z1xminb	222	8	~	~	CD
cord-299281-5z1xminb	222	9	m	m	XX
cord-299281-5z1xminb	222	10	on	on	IN
cord-299281-5z1xminb	222	11	sucrose	sucrose	NN
cord-299281-5z1xminb	222	12	gradients	gradient	NNS
cord-299281-5z1xminb	222	13	was	be	VBD
cord-299281-5z1xminb	222	14	unaltered	unaltered	JJ
cord-299281-5z1xminb	222	15	during	during	IN
cord-299281-5z1xminb	222	16	the	the	DT
cord-299281-5z1xminb	222	17	chase	chase	NN
cord-299281-5z1xminb	222	18	(	(	-LRB-
cord-299281-5z1xminb	222	19	data	datum	NNS
cord-299281-5z1xminb	222	20	not	not	RB
cord-299281-5z1xminb	222	21	shown	show	VBN
cord-299281-5z1xminb	222	22	)	)	-RRB-
cord-299281-5z1xminb	222	23	.	.	.
cord-299281-5z1xminb	223	1	Thus	thus	RB
cord-299281-5z1xminb	223	2	the	the	DT
cord-299281-5z1xminb	223	3	VSV	VSV	NNP
cord-299281-5z1xminb	223	4	G	G	NNP
cord-299281-5z1xminb	223	5	lumenal	lumenal	JJ
cord-299281-5z1xminb	223	6	domain	domain	NN
cord-299281-5z1xminb	223	7	was	be	VBD
cord-299281-5z1xminb	223	8	not	not	RB
cord-299281-5z1xminb	223	9	required	require	VBN
cord-299281-5z1xminb	223	10	for	for	IN
cord-299281-5z1xminb	223	11	SDS	SDS	NNP
cord-299281-5z1xminb	223	12	-	-	HYPH
cord-299281-5z1xminb	223	13	resistant	resistant	JJ
cord-299281-5z1xminb	223	14	oligomer	oligomer	NNP
cord-299281-5z1xminb	223	15	formation	formation	NN
cord-299281-5z1xminb	223	16	.	.	.
cord-299281-5z1xminb	224	1	We	-PRON-	PRP
cord-299281-5z1xminb	224	2	used	use	VBD
cord-299281-5z1xminb	224	3	a	a	DT
cord-299281-5z1xminb	224	4	coprecipitation	coprecipitation	NN
cord-299281-5z1xminb	224	5	assay	assay	NN
cord-299281-5z1xminb	224	6	to	to	TO
cord-299281-5z1xminb	224	7	ask	ask	VB
cord-299281-5z1xminb	224	8	if	if	IN
cord-299281-5z1xminb	224	9	otmlG	otmlG	NNP
cord-299281-5z1xminb	224	10	formed	form	VBD
cord-299281-5z1xminb	224	11	hetero	hetero	NNS
cord-299281-5z1xminb	224	12	-	-	NNS
cord-299281-5z1xminb	224	13	oligomers	oligomer	NNS
cord-299281-5z1xminb	224	14	with	with	IN
cord-299281-5z1xminb	224	15	Gml	Gml	NNP
cord-299281-5z1xminb	224	16	or	or	CC
cord-299281-5z1xminb	224	17	other	other	JJ
cord-299281-5z1xminb	224	18	related	relate	VBN
cord-299281-5z1xminb	224	19	Golgi	golgi	NN
cord-299281-5z1xminb	224	20	-	-	HYPH
cord-299281-5z1xminb	224	21	resident	resident	NN
cord-299281-5z1xminb	224	22	proteins	protein	NNS
cord-299281-5z1xminb	224	23	.	.	.
cord-299281-5z1xminb	225	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	225	2	cells	cell	NNS
cord-299281-5z1xminb	225	3	were	be	VBD
cord-299281-5z1xminb	225	4	cotransfected	cotransfecte	VBN
cord-299281-5z1xminb	225	5	to	to	TO
cord-299281-5z1xminb	225	6	express	express	VB
cord-299281-5z1xminb	225	7	both	both	DT
cord-299281-5z1xminb	225	8	otmlG	otmlG	NNP
cord-299281-5z1xminb	225	9	and	and	CC
cord-299281-5z1xminb	225	10	either	either	CC
cord-299281-5z1xminb	225	11	Gml	Gml	NNP
cord-299281-5z1xminb	225	12	,	,	,
cord-299281-5z1xminb	225	13	Gmlins	Gmlins	NNPS
cord-299281-5z1xminb	225	14	,	,	,
cord-299281-5z1xminb	225	15	or	or	CC
cord-299281-5z1xminb	225	16	VSV	VSV	NNP
cord-299281-5z1xminb	225	17	G.	G.	NNP
cord-299281-5z1xminb	226	1	After	after	IN
cord-299281-5z1xminb	226	2	a	a	DT
cord-299281-5z1xminb	226	3	short	short	JJ
cord-299281-5z1xminb	226	4	metabolic	metabolic	JJ
cord-299281-5z1xminb	226	5	labeling	labeling	NN
cord-299281-5z1xminb	226	6	period	period	NN
cord-299281-5z1xminb	226	7	(	(	-LRB-
cord-299281-5z1xminb	226	8	5	5	CD
cord-299281-5z1xminb	226	9	min	min	NNP
cord-299281-5z1xminb	226	10	)	)	-RRB-
cord-299281-5z1xminb	226	11	,	,	,
cord-299281-5z1xminb	226	12	cells	cell	NNS
cord-299281-5z1xminb	226	13	were	be	VBD
cord-299281-5z1xminb	226	14	solubilized	solubilize	VBN
cord-299281-5z1xminb	226	15	either	either	CC
cord-299281-5z1xminb	226	16	immediately	immediately	RB
cord-299281-5z1xminb	226	17	or	or	CC
cord-299281-5z1xminb	226	18	after	after	IN
cord-299281-5z1xminb	226	19	a	a	DT
cord-299281-5z1xminb	226	20	60-min	60-min	CD
cord-299281-5z1xminb	226	21	chase	chase	NN
cord-299281-5z1xminb	226	22	.	.	.
cord-299281-5z1xminb	227	1	Lysates	Lysates	NNP
cord-299281-5z1xminb	227	2	were	be	VBD
cord-299281-5z1xminb	227	3	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	227	4	using	use	VBG
cord-299281-5z1xminb	227	5	anti	anti	JJ
cord-299281-5z1xminb	227	6	-	-	JJ
cord-299281-5z1xminb	227	7	hCG	hcg	JJ
cord-299281-5z1xminb	227	8	antibody	antibody	NN
cord-299281-5z1xminb	227	9	.	.	.
cord-299281-5z1xminb	228	1	When	when	WRB
cord-299281-5z1xminb	228	2	otmlG	otmlG	NNP
cord-299281-5z1xminb	228	3	was	be	VBD
cord-299281-5z1xminb	228	4	expressed	express	VBN
cord-299281-5z1xminb	228	5	alone	alone	JJ
cord-299281-5z1xminb	228	6	,	,	,
cord-299281-5z1xminb	228	7	the	the	DT
cord-299281-5z1xminb	228	8	time	time	NN
cord-299281-5z1xminb	228	9	-	-	HYPH
cord-299281-5z1xminb	228	10	dependent	dependent	JJ
cord-299281-5z1xminb	228	11	accumulation	accumulation	NN
cord-299281-5z1xminb	228	12	of	of	IN
cord-299281-5z1xminb	228	13	SDS	SDS	NNP
cord-299281-5z1xminb	228	14	-	-	HYPH
cord-299281-5z1xminb	228	15	resistant	resistant	JJ
cord-299281-5z1xminb	228	16	oligomer	oligomer	NNP
cord-299281-5z1xminb	228	17	at	at	IN
cord-299281-5z1xminb	228	18	the	the	DT
cord-299281-5z1xminb	228	19	gel	gel	NN
cord-299281-5z1xminb	228	20	interface	interface	NN
cord-299281-5z1xminb	228	21	(	(	-LRB-
cord-299281-5z1xminb	228	22	arrowhead	arrowhead	NNP
cord-299281-5z1xminb	228	23	)	)	-RRB-
cord-299281-5z1xminb	228	24	was	be	VBD
cord-299281-5z1xminb	228	25	observed	observe	VBN
cord-299281-5z1xminb	228	26	as	as	IN
cord-299281-5z1xminb	228	27	described	describe	VBN
cord-299281-5z1xminb	228	28	above	above	IN
cord-299281-5z1xminb	228	29	(	(	-LRB-
cord-299281-5z1xminb	228	30	Fig	Fig	NNP
cord-299281-5z1xminb	228	31	.	.	NNP
cord-299281-5z1xminb	228	32	7	7	CD
cord-299281-5z1xminb	228	33	,	,	,
cord-299281-5z1xminb	228	34	lane	lane	NN
cord-299281-5z1xminb	228	35	2	2	CD
cord-299281-5z1xminb	228	36	)	)	-RRB-
cord-299281-5z1xminb	228	37	.	.	.
cord-299281-5z1xminb	229	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	229	2	,	,	,
cord-299281-5z1xminb	229	3	when	when	WRB
cord-299281-5z1xminb	229	4	otmlG	otmlG	NNP
cord-299281-5z1xminb	229	5	was	be	VBD
cord-299281-5z1xminb	229	6	coexpressed	coexpresse	VBN
cord-299281-5z1xminb	229	7	with	with	IN
cord-299281-5z1xminb	229	8	Gml	Gml	NNP
cord-299281-5z1xminb	229	9	or	or	CC
cord-299281-5z1xminb	229	10	Gmlins	Gmlins	NNPS
cord-299281-5z1xminb	229	11	,	,	,
cord-299281-5z1xminb	229	12	the	the	DT
cord-299281-5z1xminb	229	13	size	size	NN
cord-299281-5z1xminb	229	14	of	of	IN
cord-299281-5z1xminb	229	15	the	the	DT
cord-299281-5z1xminb	229	16	oligomer	oligomer	NN
cord-299281-5z1xminb	229	17	shifted	shift	VBD
cord-299281-5z1xminb	229	18	dramatically	dramatically	RB
cord-299281-5z1xminb	229	19	,	,	,
cord-299281-5z1xminb	229	20	and	and	CC
cord-299281-5z1xminb	229	21	now	now	RB
cord-299281-5z1xminb	229	22	migated	migate	VBN
cord-299281-5z1xminb	229	23	at	at	IN
cord-299281-5z1xminb	229	24	the	the	DT
cord-299281-5z1xminb	229	25	top	top	NN
cord-299281-5z1xminb	229	26	of	of	IN
cord-299281-5z1xminb	229	27	the	the	DT
cord-299281-5z1xminb	229	28	stacking	stacking	NN
cord-299281-5z1xminb	229	29	gel	gel	NN
cord-299281-5z1xminb	229	30	(	(	-LRB-
cord-299281-5z1xminb	229	31	Fig	Fig	NNP
cord-299281-5z1xminb	229	32	.	.	NNP
cord-299281-5z1xminb	229	33	7	7	CD
cord-299281-5z1xminb	229	34	,	,	,
cord-299281-5z1xminb	229	35	lanes	lane	NNS
cord-299281-5z1xminb	229	36	4	4	CD
cord-299281-5z1xminb	229	37	and	and	CC
cord-299281-5z1xminb	229	38	8)	8)	NN
cord-299281-5z1xminb	229	39	.	.	.
cord-299281-5z1xminb	230	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	230	2	,	,	,
cord-299281-5z1xminb	230	3	some	some	DT
cord-299281-5z1xminb	230	4	proteins	protein	NNS
cord-299281-5z1xminb	230	5	appeared	appear	VBD
cord-299281-5z1xminb	230	6	to	to	TO
cord-299281-5z1xminb	230	7	be	be	VB
cord-299281-5z1xminb	230	8	solubilized	solubilize	VBN
cord-299281-5z1xminb	230	9	during	during	IN
cord-299281-5z1xminb	230	10	electrophoresis	electrophoresis	NN
cord-299281-5z1xminb	230	11	,	,	,
cord-299281-5z1xminb	230	12	since	since	IN
cord-299281-5z1xminb	230	13	a	a	DT
cord-299281-5z1xminb	230	14	small	small	JJ
cord-299281-5z1xminb	230	15	amount	amount	NN
cord-299281-5z1xminb	230	16	of	of	IN
cord-299281-5z1xminb	230	17	material	material	NN
cord-299281-5z1xminb	230	18	migrating	migrate	VBG
cord-299281-5z1xminb	230	19	with	with	IN
cord-299281-5z1xminb	230	20	authentic	authentic	JJ
cord-299281-5z1xminb	230	21	Gml	Gml	NNP
cord-299281-5z1xminb	230	22	and	and	CC
cord-299281-5z1xminb	230	23	Gmlins	Gmlins	NNPS
cord-299281-5z1xminb	230	24	w	w	NNP
cord-299281-5z1xminb	230	25	a	a	DT
cord-299281-5z1xminb	230	26	s	s	NN
cord-299281-5z1xminb	230	27	alSO	alSO	VBN
cord-299281-5z1xminb	230	28	detected	detect	VBN
cord-299281-5z1xminb	230	29	in	in	IN
cord-299281-5z1xminb	230	30	these	these	DT
cord-299281-5z1xminb	230	31	lanes	lane	NNS
cord-299281-5z1xminb	230	32	(	(	-LRB-
cord-299281-5z1xminb	230	33	Fig	Fig	NNP
cord-299281-5z1xminb	230	34	.	.	NNP
cord-299281-5z1xminb	230	35	7	7	CD
cord-299281-5z1xminb	230	36	,	,	,
cord-299281-5z1xminb	230	37	arrow	arrow	NN
cord-299281-5z1xminb	230	38	)	)	-RRB-
cord-299281-5z1xminb	230	39	.	.	.
cord-299281-5z1xminb	231	1	In	in	IN
cord-299281-5z1xminb	231	2	contrast	contrast	NN
cord-299281-5z1xminb	231	3	,	,	,
cord-299281-5z1xminb	231	4	no	no	DT
cord-299281-5z1xminb	231	5	change	change	NN
cord-299281-5z1xminb	231	6	in	in	IN
cord-299281-5z1xminb	231	7	the	the	DT
cord-299281-5z1xminb	231	8	size	size	NN
cord-299281-5z1xminb	231	9	of	of	IN
cord-299281-5z1xminb	231	10	the	the	DT
cord-299281-5z1xminb	231	11	oligomer	oligomer	NNP
cord-299281-5z1xminb	231	12	was	be	VBD
cord-299281-5z1xminb	231	13	observed	observe	VBN
cord-299281-5z1xminb	231	14	when	when	WRB
cord-299281-5z1xminb	231	15	c	c	NNP
cord-299281-5z1xminb	231	16	~	~	NFP
cord-299281-5z1xminb	231	17	mlG	mlG	-LRB-
cord-299281-5z1xminb	231	18	was	be	VBD
cord-299281-5z1xminb	231	19	coexpressed	coexpresse	VBN
cord-299281-5z1xminb	231	20	with	with	IN
cord-299281-5z1xminb	231	21	VSV	VSV	NNP
cord-299281-5z1xminb	231	22	G	G	NNP
cord-299281-5z1xminb	231	23	(	(	-LRB-
cord-299281-5z1xminb	231	24	Fig	Fig	NNP
cord-299281-5z1xminb	231	25	.	.	NNP
cord-299281-5z1xminb	231	26	7	7	CD
cord-299281-5z1xminb	231	27	,	,	,
cord-299281-5z1xminb	231	28	lane	lane	NN
cord-299281-5z1xminb	231	29	6	6	CD
cord-299281-5z1xminb	231	30	)	)	-RRB-
cord-299281-5z1xminb	231	31	or	or	CC
cord-299281-5z1xminb	231	32	with	with	IN
cord-299281-5z1xminb	231	33	GmlQLs0	GmlQLs0	NNP
cord-299281-5z1xminb	232	1	(	(	-LRB-
cord-299281-5z1xminb	232	2	data	datum	NNS
cord-299281-5z1xminb	232	3	not	not	RB
cord-299281-5z1xminb	232	4	shown	show	VBN
cord-299281-5z1xminb	232	5	)	)	-RRB-
cord-299281-5z1xminb	232	6	,	,	,
cord-299281-5z1xminb	232	7	which	which	WDT
cord-299281-5z1xminb	232	8	are	be	VBP
cord-299281-5z1xminb	232	9	transported	transport	VBN
cord-299281-5z1xminb	232	10	to	to	IN
cord-299281-5z1xminb	232	11	the	the	DT
cord-299281-5z1xminb	232	12	plasma	plasma	NN
cord-299281-5z1xminb	232	13	membrane	membrane	NN
cord-299281-5z1xminb	232	14	.	.	.
cord-299281-5z1xminb	233	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	233	2	,	,	,
cord-299281-5z1xminb	233	3	when	when	WRB
cord-299281-5z1xminb	233	4	cells	cell	NNS
cord-299281-5z1xminb	233	5	expressing	express	VBG
cord-299281-5z1xminb	233	6	Gml	Gml	NNP
cord-299281-5z1xminb	233	7	and	and	CC
cord-299281-5z1xminb	233	8	c	c	NN
cord-299281-5z1xminb	233	9	~	~	NFP
cord-299281-5z1xminb	233	10	m	m	NNP
cord-299281-5z1xminb	233	11	(	(	-LRB-
cord-299281-5z1xminb	233	12	containing	contain	VBG
cord-299281-5z1xminb	233	13	the	the	DT
cord-299281-5z1xminb	233	14	VSV	VSV	NNP
cord-299281-5z1xminb	233	15	G	G	NNP
cord-299281-5z1xminb	233	16	membrane	membrane	NN
cord-299281-5z1xminb	233	17	-	-	HYPH
cord-299281-5z1xminb	233	18	spanning	span	VBG
cord-299281-5z1xminb	233	19	domain	domain	NN
cord-299281-5z1xminb	233	20	)	)	-RRB-
cord-299281-5z1xminb	233	21	were	be	VBD
cord-299281-5z1xminb	233	22	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	233	23	using	use	VBG
cord-299281-5z1xminb	233	24	anti	anti	JJ
cord-299281-5z1xminb	233	25	-	-	JJ
cord-299281-5z1xminb	233	26	hCG	hcg	JJ
cord-299281-5z1xminb	233	27	antibody	antibody	NN
cord-299281-5z1xminb	233	28	,	,	,
cord-299281-5z1xminb	233	29	no	no	DT
cord-299281-5z1xminb	233	30	Gml	Gml	NNP
cord-299281-5z1xminb	233	31	was	be	VBD
cord-299281-5z1xminb	233	32	recovered	recover	VBN
cord-299281-5z1xminb	233	33	(	(	-LRB-
cord-299281-5z1xminb	233	34	data	datum	NNS
cord-299281-5z1xminb	233	35	not	not	RB
cord-299281-5z1xminb	233	36	shown	show	VBN
cord-299281-5z1xminb	233	37	)	)	-RRB-
cord-299281-5z1xminb	233	38	.	.	.
cord-299281-5z1xminb	234	1	To	to	TO
cord-299281-5z1xminb	234	2	test	test	VB
cord-299281-5z1xminb	234	3	the	the	DT
cord-299281-5z1xminb	234	4	role	role	NN
cord-299281-5z1xminb	234	5	of	of	IN
cord-299281-5z1xminb	234	6	the	the	DT
cord-299281-5z1xminb	234	7	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	234	8	tail	tail	NN
cord-299281-5z1xminb	234	9	of	of	IN
cord-299281-5z1xminb	234	10	Gml	Gml	NNP
cord-299281-5z1xminb	234	11	in	in	IN
cord-299281-5z1xminb	234	12	oligomer	oligomer	NNP
cord-299281-5z1xminb	234	13	formation	formation	NN
cord-299281-5z1xminb	234	14	,	,	,
cord-299281-5z1xminb	234	15	we	-PRON-	PRP
cord-299281-5z1xminb	234	16	inserted	insert	VBD
cord-299281-5z1xminb	234	17	a	a	DT
cord-299281-5z1xminb	234	18	stop	stop	NN
cord-299281-5z1xminb	234	19	codon	codon	NN
cord-299281-5z1xminb	234	20	after	after	IN
cord-299281-5z1xminb	234	21	the	the	DT
cord-299281-5z1xminb	234	22	first	first	JJ
cord-299281-5z1xminb	234	23	residue	residue	NN
cord-299281-5z1xminb	234	24	(	(	-LRB-
cord-299281-5z1xminb	234	25	Arg	arg	NN
cord-299281-5z1xminb	234	26	)	)	-RRB-
cord-299281-5z1xminb	234	27	beyond	beyond	IN
cord-299281-5z1xminb	234	28	the	the	DT
cord-299281-5z1xminb	234	29	transmembrane	transmembrane	NN
cord-299281-5z1xminb	234	30	domain	domain	NN
cord-299281-5z1xminb	234	31	(	(	-LRB-
cord-299281-5z1xminb	234	32	tGml	tGml	NNP
cord-299281-5z1xminb	234	33	)	)	-RRB-
cord-299281-5z1xminb	234	34	.	.	.
cord-299281-5z1xminb	235	1	The	the	DT
cord-299281-5z1xminb	235	2	corresponding	correspond	VBG
cord-299281-5z1xminb	235	3	tailless	tailless	JJ
cord-299281-5z1xminb	235	4	VSV	VSV	NNP
cord-299281-5z1xminb	235	5	G	G	NNP
cord-299281-5z1xminb	235	6	(	(	-LRB-
cord-299281-5z1xminb	235	7	TMR	TMR	NNP
cord-299281-5z1xminb	235	8	;	;	:
cord-299281-5z1xminb	235	9	Doms	Doms	NNPS
cord-299281-5z1xminb	235	10	et	et	FW
cord-299281-5z1xminb	235	11	al	al	NNP
cord-299281-5z1xminb	235	12	.	.	NNP
cord-299281-5z1xminb	235	13	,	,	,
cord-299281-5z1xminb	235	14	1988	1988	CD
cord-299281-5z1xminb	235	15	)	)	-RRB-
cord-299281-5z1xminb	235	16	trimerizes	trimerizes	VBP
cord-299281-5z1xminb	235	17	with	with	IN
cord-299281-5z1xminb	235	18	normal	normal	JJ
cord-299281-5z1xminb	235	19	kinetics	kinetic	NNS
cord-299281-5z1xminb	235	20	but	but	CC
cord-299281-5z1xminb	235	21	is	be	VBZ
cord-299281-5z1xminb	235	22	transported	transport	VBN
cord-299281-5z1xminb	235	23	slowly	slowly	RB
cord-299281-5z1xminb	235	24	from	from	IN
cord-299281-5z1xminb	235	25	the	the	DT
cord-299281-5z1xminb	235	26	ER	ER	NNP
cord-299281-5z1xminb	235	27	to	to	IN
cord-299281-5z1xminb	235	28	the	the	DT
cord-299281-5z1xminb	235	29	plasma	plasma	NN
cord-299281-5z1xminb	235	30	membrane	membrane	NN
cord-299281-5z1xminb	235	31	.	.	.
cord-299281-5z1xminb	236	1	When	when	WRB
cord-299281-5z1xminb	236	2	tGml	tGml	NNP
cord-299281-5z1xminb	236	3	was	be	VBD
cord-299281-5z1xminb	236	4	localized	localize	VBN
cord-299281-5z1xminb	236	5	by	by	IN
cord-299281-5z1xminb	236	6	Figure	Figure	NNP
cord-299281-5z1xminb	236	7	6	6	CD
cord-299281-5z1xminb	236	8	.	.	.
cord-299281-5z1xminb	237	1	amlG	amlg	LS
cord-299281-5z1xminb	237	2	forms	form	VBZ
cord-299281-5z1xminb	237	3	an	an	DT
cord-299281-5z1xminb	237	4	SDS	SDS	NNP
cord-299281-5z1xminb	237	5	-	-	HYPH
cord-299281-5z1xminb	237	6	resistant	resistant	JJ
cord-299281-5z1xminb	237	7	oligomer	oligomer	NNP
cord-299281-5z1xminb	237	8	.	.	.
cord-299281-5z1xminb	238	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	238	2	cells	cell	NNS
cord-299281-5z1xminb	238	3	transfected	transfecte	VBN
cord-299281-5z1xminb	238	4	with	with	IN
cord-299281-5z1xminb	238	5	amlG	amlg	CD
cord-299281-5z1xminb	238	6	or	or	CC
cord-299281-5z1xminb	238	7	am	be	VBP
cord-299281-5z1xminb	238	8	were	be	VBD
cord-299281-5z1xminb	238	9	metabolically	metabolically	RB
cord-299281-5z1xminb	238	10	labeled	label	VBN
cord-299281-5z1xminb	238	11	for	for	IN
cord-299281-5z1xminb	238	12	5	5	CD
cord-299281-5z1xminb	238	13	rain	rain	NN
cord-299281-5z1xminb	238	14	,	,	,
cord-299281-5z1xminb	238	15	and	and	CC
cord-299281-5z1xminb	238	16	then	then	RB
cord-299281-5z1xminb	238	17	solubilized	solubilize	VBD
cord-299281-5z1xminb	238	18	either	either	CC
cord-299281-5z1xminb	238	19	immediately	immediately	RB
cord-299281-5z1xminb	238	20	or	or	CC
cord-299281-5z1xminb	238	21	after	after	IN
cord-299281-5z1xminb	238	22	a	a	DT
cord-299281-5z1xminb	238	23	60-min	60-min	CD
cord-299281-5z1xminb	238	24	chase	chase	NN
cord-299281-5z1xminb	238	25	and	and	CC
cord-299281-5z1xminb	238	26	immunoprecipitated	immunoprecipitate	VBD
cord-299281-5z1xminb	238	27	with	with	IN
cord-299281-5z1xminb	238	28	anti	anti	JJ
cord-299281-5z1xminb	238	29	-	-	JJ
cord-299281-5z1xminb	238	30	hCG	hcg	JJ
cord-299281-5z1xminb	238	31	antibody	antibody	NN
cord-299281-5z1xminb	238	32	.	.	.
cord-299281-5z1xminb	239	1	Samples	sample	NNS
cord-299281-5z1xminb	239	2	were	be	VBD
cord-299281-5z1xminb	239	3	analyzed	analyze	VBN
cord-299281-5z1xminb	239	4	by	by	IN
cord-299281-5z1xminb	239	5	SDS	SDS	NNP
cord-299281-5z1xminb	239	6	-	-	HYPH
cord-299281-5z1xminb	239	7	PAGE	PAGE	NNP
cord-299281-5z1xminb	239	8	on	on	IN
cord-299281-5z1xminb	239	9	15	15	CD
cord-299281-5z1xminb	239	10	%	%	NN
cord-299281-5z1xminb	239	11	gels	gel	NNS
cord-299281-5z1xminb	239	12	.	.	.
cord-299281-5z1xminb	240	1	Note	note	VB
cord-299281-5z1xminb	240	2	the	the	DT
cord-299281-5z1xminb	240	3	time	time	NN
cord-299281-5z1xminb	240	4	-	-	HYPH
cord-299281-5z1xminb	240	5	dependent	dependent	JJ
cord-299281-5z1xminb	240	6	accumulation	accumulation	NN
cord-299281-5z1xminb	240	7	of	of	IN
cord-299281-5z1xminb	240	8	an	an	DT
cord-299281-5z1xminb	240	9	SDS	sds	NN
cord-299281-5z1xminb	240	10	-	-	HYPH
cord-299281-5z1xminb	240	11	resistant	resistant	JJ
cord-299281-5z1xminb	240	12	species	specie	NNS
cord-299281-5z1xminb	240	13	at	at	IN
cord-299281-5z1xminb	240	14	the	the	DT
cord-299281-5z1xminb	240	15	interface	interface	NN
cord-299281-5z1xminb	240	16	between	between	IN
cord-299281-5z1xminb	240	17	the	the	DT
cord-299281-5z1xminb	240	18	separating	separate	VBG
cord-299281-5z1xminb	240	19	and	and	CC
cord-299281-5z1xminb	240	20	stacking	stack	VBG
cord-299281-5z1xminb	240	21	gels	gel	NNS
cord-299281-5z1xminb	240	22	(	(	-LRB-
cord-299281-5z1xminb	240	23	arrowhead	arrowhead	NNP
cord-299281-5z1xminb	240	24	)	)	-RRB-
cord-299281-5z1xminb	240	25	.	.	.
cord-299281-5z1xminb	241	1	The	the	DT
cord-299281-5z1xminb	241	2	increased	increase	VBN
cord-299281-5z1xminb	241	3	mobility	mobility	NN
cord-299281-5z1xminb	241	4	of	of	IN
cord-299281-5z1xminb	241	5	amlG	amlg	NN
cord-299281-5z1xminb	241	6	and	and	CC
cord-299281-5z1xminb	241	7	am	be	VBP
cord-299281-5z1xminb	241	8	after	after	IN
cord-299281-5z1xminb	241	9	60	60	CD
cord-299281-5z1xminb	241	10	min	min	NN
cord-299281-5z1xminb	241	11	of	of	IN
cord-299281-5z1xminb	241	12	chase	chase	NN
cord-299281-5z1xminb	241	13	is	be	VBZ
cord-299281-5z1xminb	241	14	due	due	JJ
cord-299281-5z1xminb	241	15	to	to	IN
cord-299281-5z1xminb	241	16	carbohydrate	carbohydrate	NN
cord-299281-5z1xminb	241	17	trimming	trimming	NN
cord-299281-5z1xminb	241	18	.	.	.
cord-299281-5z1xminb	242	1	indirect	indirect	JJ
cord-299281-5z1xminb	242	2	immunofluorescence	immunofluorescence	NN
cord-299281-5z1xminb	242	3	in	in	IN
cord-299281-5z1xminb	242	4	transiently	transiently	RB
cord-299281-5z1xminb	242	5	transfected	transfecte	VBN
cord-299281-5z1xminb	242	6	COS-7	COS-7	NNP
cord-299281-5z1xminb	242	7	cells	cell	NNS
cord-299281-5z1xminb	242	8	,	,	,
cord-299281-5z1xminb	242	9	some	some	DT
cord-299281-5z1xminb	242	10	Golgi	Golgi	NNP
cord-299281-5z1xminb	242	11	staining	staining	NN
cord-299281-5z1xminb	242	12	was	be	VBD
cord-299281-5z1xminb	242	13	observed	observe	VBN
cord-299281-5z1xminb	242	14	,	,	,
cord-299281-5z1xminb	242	15	although	although	IN
cord-299281-5z1xminb	242	16	a	a	DT
cord-299281-5z1xminb	242	17	significant	significant	JJ
cord-299281-5z1xminb	242	18	amount	amount	NN
cord-299281-5z1xminb	242	19	of	of	IN
cord-299281-5z1xminb	242	20	the	the	DT
cord-299281-5z1xminb	242	21	protein	protein	NN
cord-299281-5z1xminb	242	22	was	be	VBD
cord-299281-5z1xminb	242	23	found	find	VBN
cord-299281-5z1xminb	242	24	in	in	IN
cord-299281-5z1xminb	242	25	the	the	DT
cord-299281-5z1xminb	242	26	ER	ER	NNP
cord-299281-5z1xminb	242	27	.	.	.
cord-299281-5z1xminb	243	1	Unlike	unlike	IN
cord-299281-5z1xminb	243	2	Grid	Grid	NNP
cord-299281-5z1xminb	243	3	,	,	,
cord-299281-5z1xminb	243	4	tGml	tGml	NNP
cord-299281-5z1xminb	243	5	did	do	VBD
cord-299281-5z1xminb	243	6	not	not	RB
cord-299281-5z1xminb	243	7	form	form	VB
cord-299281-5z1xminb	243	8	an	an	DT
cord-299281-5z1xminb	243	9	SDS	sds	NN
cord-299281-5z1xminb	243	10	-	-	HYPH
cord-299281-5z1xminb	243	11	resistant	resistant	JJ
cord-299281-5z1xminb	243	12	oligomer	oligomer	NNP
cord-299281-5z1xminb	243	13	when	when	WRB
cord-299281-5z1xminb	243	14	expressed	express	VBN
cord-299281-5z1xminb	243	15	in	in	IN
cord-299281-5z1xminb	243	16	COS-'/or	COS-'/or	NNP
cord-299281-5z1xminb	243	17	HeLa	HeLa	NNP
cord-299281-5z1xminb	243	18	cells	cell	NNS
cord-299281-5z1xminb	243	19	,	,	,
cord-299281-5z1xminb	243	20	and	and	CC
cord-299281-5z1xminb	243	21	did	do	VBD
cord-299281-5z1xminb	243	22	not	not	RB
cord-299281-5z1xminb	243	23	sediment	sediment	VB
cord-299281-5z1xminb	243	24	as	as	IN
cord-299281-5z1xminb	243	25	a	a	DT
cord-299281-5z1xminb	243	26	large	large	JJ
cord-299281-5z1xminb	243	27	oligomer	oligomer	NN
cord-299281-5z1xminb	243	28	in	in	IN
cord-299281-5z1xminb	243	29	sucrose	sucrose	NN
cord-299281-5z1xminb	243	30	gradients	gradient	NNS
cord-299281-5z1xminb	243	31	(	(	-LRB-
cord-299281-5z1xminb	243	32	although	although	IN
cord-299281-5z1xminb	243	33	it	-PRON-	PRP
cord-299281-5z1xminb	243	34	did	do	VBD
cord-299281-5z1xminb	243	35	apparently	apparently	RB
cord-299281-5z1xminb	243	36	form	form	VB
cord-299281-5z1xminb	243	37	trimers	trimer	NNS
cord-299281-5z1xminb	243	38	,	,	,
cord-299281-5z1xminb	243	39	not	not	RB
cord-299281-5z1xminb	243	40	shown	show	VBN
cord-299281-5z1xminb	243	41	)	)	-RRB-
cord-299281-5z1xminb	243	42	.	.	.
cord-299281-5z1xminb	244	1	However	however	RB
cord-299281-5z1xminb	244	2	,	,	,
cord-299281-5z1xminb	244	3	because	because	IN
cord-299281-5z1xminb	244	4	tGml	tGml	NNP
cord-299281-5z1xminb	244	5	appears	appear	VBZ
cord-299281-5z1xminb	244	6	to	to	TO
cord-299281-5z1xminb	244	7	exit	exit	VB
cord-299281-5z1xminb	244	8	the	the	DT
cord-299281-5z1xminb	244	9	ER	ER	NNP
cord-299281-5z1xminb	244	10	very	very	RB
cord-299281-5z1xminb	244	11	slowly	slowly	RB
cord-299281-5z1xminb	244	12	(	(	-LRB-
cord-299281-5z1xminb	244	13	like	like	IN
cord-299281-5z1xminb	244	14	TMR	TMR	NNP
cord-299281-5z1xminb	244	15	)	)	-RRB-
cord-299281-5z1xminb	244	16	,	,	,
cord-299281-5z1xminb	244	17	the	the	DT
cord-299281-5z1xminb	244	18	kinetics	kinetics	NNP
cord-299281-5z1xminb	244	19	of	of	IN
cord-299281-5z1xminb	244	20	oligomer	oligomer	NNP
cord-299281-5z1xminb	244	21	formation	formation	NN
cord-299281-5z1xminb	244	22	may	may	MD
cord-299281-5z1xminb	244	23	have	have	VB
cord-299281-5z1xminb	244	24	been	be	VBN
cord-299281-5z1xminb	244	25	too	too	RB
cord-299281-5z1xminb	244	26	slow	slow	JJ
cord-299281-5z1xminb	244	27	to	to	TO
cord-299281-5z1xminb	244	28	detect	detect	VB
cord-299281-5z1xminb	244	29	.	.	.
cord-299281-5z1xminb	245	1	Because	because	IN
cord-299281-5z1xminb	245	2	tGml	tGml	NNP
cord-299281-5z1xminb	245	3	did	do	VBD
cord-299281-5z1xminb	245	4	not	not	RB
cord-299281-5z1xminb	245	5	form	form	VB
cord-299281-5z1xminb	245	6	detectable	detectable	JJ
cord-299281-5z1xminb	245	7	oligomers	oligomer	NNS
cord-299281-5z1xminb	245	8	,	,	,
cord-299281-5z1xminb	245	9	we	-PRON-	PRP
cord-299281-5z1xminb	245	10	asked	ask	VBD
cord-299281-5z1xminb	245	11	whether	whether	IN
cord-299281-5z1xminb	245	12	preformed	preformed	JJ
cord-299281-5z1xminb	245	13	Gml	Gml	NNP
cord-299281-5z1xminb	245	14	oligomers	oligomer	NNS
cord-299281-5z1xminb	245	15	could	could	MD
cord-299281-5z1xminb	245	16	be	be	VB
cord-299281-5z1xminb	245	17	disrupted	disrupt	VBN
cord-299281-5z1xminb	245	18	by	by	IN
cord-299281-5z1xminb	245	19	proteolysis	proteolysis	NN
cord-299281-5z1xminb	245	20	of	of	IN
cord-299281-5z1xminb	245	21	the	the	DT
cord-299281-5z1xminb	245	22	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	245	23	tall	tall	JJ
cord-299281-5z1xminb	245	24	.	.	.
cord-299281-5z1xminb	246	1	The	the	DT
cord-299281-5z1xminb	246	2	29-amino	29-amino	CD
cord-299281-5z1xminb	246	3	acid	acid	NN
cord-299281-5z1xminb	246	4	Figure	figure	NN
cord-299281-5z1xminb	246	5	7	7	CD
cord-299281-5z1xminb	246	6	.	.	.
cord-299281-5z1xminb	247	1	Gml	Gml	NNP
cord-299281-5z1xminb	247	2	and	and	CC
cord-299281-5z1xminb	247	3	c	c	NN
cord-299281-5z1xminb	247	4	~	~	NFP
cord-299281-5z1xminb	247	5	mlG	mlG	-LRB-
cord-299281-5z1xminb	247	6	form	form	NN
cord-299281-5z1xminb	247	7	hetero	hetero	NNS
cord-299281-5z1xminb	247	8	-	-	NNS
cord-299281-5z1xminb	247	9	oligomers	oligomer	NNS
cord-299281-5z1xminb	247	10	.	.	.
cord-299281-5z1xminb	248	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	248	2	cells	cell	NNS
cord-299281-5z1xminb	248	3	were	be	VBD
cord-299281-5z1xminb	248	4	transfected	transfecte	VBN
cord-299281-5z1xminb	248	5	with	with	IN
cord-299281-5z1xminb	248	6	1	1	CD
cord-299281-5z1xminb	248	7	#	#	$
cord-299281-5z1xminb	248	8	g	g	NN
cord-299281-5z1xminb	248	9	each	each	DT
cord-299281-5z1xminb	248	10	of	of	IN
cord-299281-5z1xminb	248	11	mlllG	mlllG	NNP
cord-299281-5z1xminb	248	12	and	and	CC
cord-299281-5z1xminb	248	13	either	either	CC
cord-299281-5z1xminb	248	14	calf	calf	NN
cord-299281-5z1xminb	248	15	thymus	thymus	NN
cord-299281-5z1xminb	248	16	DNA	dna	NN
cord-299281-5z1xminb	248	17	(	(	-LRB-
cord-299281-5z1xminb	248	18	CT	CT	NNP
cord-299281-5z1xminb	248	19	)	)	-RRB-
cord-299281-5z1xminb	248	20	,	,	,
cord-299281-5z1xminb	248	21	Gml	Gml	NNP
cord-299281-5z1xminb	248	22	,	,	,
cord-299281-5z1xminb	248	23	VSV	VSV	NNP
cord-299281-5z1xminb	248	24	G	G	NNP
cord-299281-5z1xminb	248	25	,	,	,
cord-299281-5z1xminb	248	26	or	or	CC
cord-299281-5z1xminb	248	27	Gml	Gml	NNP
cord-299281-5z1xminb	248	28	~	~	NFP
cord-299281-5z1xminb	248	29	n~.	n~.	XX
cord-299281-5z1xminb	249	1	After	after	IN
cord-299281-5z1xminb	249	2	a	a	DT
cord-299281-5z1xminb	249	3	5-min	5-min	JJ
cord-299281-5z1xminb	249	4	pulse	pulse	NN
cord-299281-5z1xminb	249	5	,	,	,
cord-299281-5z1xminb	249	6	cells	cell	NNS
cord-299281-5z1xminb	249	7	were	be	VBD
cord-299281-5z1xminb	249	8	solubilized	solubilize	VBN
cord-299281-5z1xminb	249	9	either	either	CC
cord-299281-5z1xminb	249	10	immediately	immediately	RB
cord-299281-5z1xminb	249	11	or	or	CC
cord-299281-5z1xminb	249	12	after	after	IN
cord-299281-5z1xminb	249	13	a	a	DT
cord-299281-5z1xminb	249	14	60-min	60-min	CD
cord-299281-5z1xminb	249	15	chase	chase	NN
cord-299281-5z1xminb	249	16	.	.	.
cord-299281-5z1xminb	250	1	Aliquots	Aliquots	NNP
cord-299281-5z1xminb	250	2	of	of	IN
cord-299281-5z1xminb	250	3	the	the	DT
cord-299281-5z1xminb	250	4	solubilized	solubilized	JJ
cord-299281-5z1xminb	250	5	cells	cell	NNS
cord-299281-5z1xminb	250	6	were	be	VBD
cord-299281-5z1xminb	250	7	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	250	8	with	with	IN
cord-299281-5z1xminb	250	9	anti	anti	JJ
cord-299281-5z1xminb	250	10	-	-	JJ
cord-299281-5z1xminb	250	11	hCG	hcg	JJ
cord-299281-5z1xminb	250	12	antibody	antibody	NN
cord-299281-5z1xminb	250	13	and	and	CC
cord-299281-5z1xminb	250	14	elcctrophoresed	elcctrophorese	VBD
cord-299281-5z1xminb	250	15	on	on	IN
cord-299281-5z1xminb	250	16	12	12	CD
cord-299281-5z1xminb	250	17	%	%	NN
cord-299281-5z1xminb	250	18	SDS	sds	NN
cord-299281-5z1xminb	250	19	-	-	HYPH
cord-299281-5z1xminb	250	20	polyacrylamide	polyacrylamide	NN
cord-299281-5z1xminb	250	21	gels	gel	NNS
cord-299281-5z1xminb	250	22	.	.	.
cord-299281-5z1xminb	251	1	The	the	DT
cord-299281-5z1xminb	251	2	interface	interface	NN
cord-299281-5z1xminb	251	3	between	between	IN
cord-299281-5z1xminb	251	4	the	the	DT
cord-299281-5z1xminb	251	5	stacking	stacking	NN
cord-299281-5z1xminb	251	6	and	and	CC
cord-299281-5z1xminb	251	7	separating	separate	VBG
cord-299281-5z1xminb	251	8	gels	gel	NNS
cord-299281-5z1xminb	251	9	is	be	VBZ
cord-299281-5z1xminb	251	10	marked	mark	VBN
cord-299281-5z1xminb	251	11	with	with	IN
cord-299281-5z1xminb	251	12	an	an	DT
cord-299281-5z1xminb	251	13	arrowhead	arrowhead	NN
cord-299281-5z1xminb	251	14	,	,	,
cord-299281-5z1xminb	251	15	and	and	CC
cord-299281-5z1xminb	251	16	the	the	DT
cord-299281-5z1xminb	251	17	mobility	mobility	NN
cord-299281-5z1xminb	251	18	of	of	IN
cord-299281-5z1xminb	251	19	Gml	Gml	NNP
cord-299281-5z1xminb	251	20	in	in	IN
cord-299281-5z1xminb	251	21	the	the	DT
cord-299281-5z1xminb	251	22	gel	gel	NN
cord-299281-5z1xminb	251	23	is	be	VBZ
cord-299281-5z1xminb	251	24	denoted	denote	VBN
cord-299281-5z1xminb	251	25	with	with	IN
cord-299281-5z1xminb	251	26	an	an	DT
cord-299281-5z1xminb	251	27	arrow	arrow	NN
cord-299281-5z1xminb	251	28	,	,	,
cord-299281-5z1xminb	251	29	amIG	amig	JJ
cord-299281-5z1xminb	251	30	forms	form	VBZ
cord-299281-5z1xminb	251	31	hetero	hetero	NNS
cord-299281-5z1xminb	251	32	-	-	HYPH
cord-299281-5z1xminb	251	33	oligomers	oligomer	NNS
cord-299281-5z1xminb	251	34	with	with	IN
cord-299281-5z1xminb	251	35	Gml	Gml	NNP
cord-299281-5z1xminb	251	36	and	and	CC
cord-299281-5z1xminb	251	37	Gmlm	Gmlm	NNP
cord-299281-5z1xminb	251	38	(	(	-LRB-
cord-299281-5z1xminb	251	39	but	but	CC
cord-299281-5z1xminb	251	40	not	not	RB
cord-299281-5z1xminb	251	41	with	with	IN
cord-299281-5z1xminb	251	42	VSV	VSV	NNP
cord-299281-5z1xminb	251	43	G	G	NNP
cord-299281-5z1xminb	251	44	)	)	-RRB-
cord-299281-5z1xminb	251	45	that	that	WDT
cord-299281-5z1xminb	251	46	could	could	MD
cord-299281-5z1xminb	251	47	be	be	VB
cord-299281-5z1xminb	251	48	precipitated	precipitate	VBN
cord-299281-5z1xminb	251	49	by	by	IN
cord-299281-5z1xminb	251	50	anti	anti	JJ
cord-299281-5z1xminb	251	51	-	-	JJ
cord-299281-5z1xminb	251	52	hCG	hcg	JJ
cord-299281-5z1xminb	251	53	antibody	antibody	NN
cord-299281-5z1xminb	251	54	.	.	.
cord-299281-5z1xminb	252	1	Fig	Fig	NNP
cord-299281-5z1xminb	252	2	.	.	.
cord-299281-5z1xminb	252	3	8	8	CD
cord-299281-5z1xminb	252	4	A	a	NN
cord-299281-5z1xminb	252	5	)	)	-RRB-
cord-299281-5z1xminb	252	6	has	have	VBZ
cord-299281-5z1xminb	252	7	numerous	numerous	JJ
cord-299281-5z1xminb	252	8	arginines	arginine	NNS
cord-299281-5z1xminb	252	9	and	and	CC
cord-299281-5z1xminb	252	10	lysines	lysine	NNS
cord-299281-5z1xminb	252	11	that	that	WDT
cord-299281-5z1xminb	252	12	are	be	VBP
cord-299281-5z1xminb	252	13	susceptible	susceptible	JJ
cord-299281-5z1xminb	252	14	to	to	IN
cord-299281-5z1xminb	252	15	proteolysis	proteolysis	NN
cord-299281-5z1xminb	252	16	by	by	IN
cord-299281-5z1xminb	252	17	trypsin	trypsin	NN
cord-299281-5z1xminb	252	18	.	.	.
cord-299281-5z1xminb	253	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	253	2	cells	cell	NNS
cord-299281-5z1xminb	253	3	expressing	express	VBG
cord-299281-5z1xminb	253	4	Gml	Gml	NNP
cord-299281-5z1xminb	253	5	or	or	CC
cord-299281-5z1xminb	253	6	VSV	VSV	NNP
cord-299281-5z1xminb	253	7	G	G	NNP
cord-299281-5z1xminb	253	8	were	be	VBD
cord-299281-5z1xminb	253	9	metabolically	metabolically	RB
cord-299281-5z1xminb	253	10	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	253	11	for	for	IN
cord-299281-5z1xminb	253	12	5	5	CD
cord-299281-5z1xminb	253	13	min	min	NN
cord-299281-5z1xminb	253	14	,	,	,
cord-299281-5z1xminb	253	15	and	and	CC
cord-299281-5z1xminb	253	16	then	then	RB
cord-299281-5z1xminb	253	17	chased	chase	VBD
cord-299281-5z1xminb	253	18	for	for	IN
cord-299281-5z1xminb	253	19	0	0	CD
cord-299281-5z1xminb	253	20	or	or	CC
cord-299281-5z1xminb	253	21	60	60	CD
cord-299281-5z1xminb	253	22	rain	rain	NN
cord-299281-5z1xminb	253	23	,	,	,
cord-299281-5z1xminb	253	24	and	and	CC
cord-299281-5z1xminb	253	25	microsomes	microsome	NNS
cord-299281-5z1xminb	253	26	prepared	prepare	VBD
cord-299281-5z1xminb	253	27	as	as	IN
cord-299281-5z1xminb	253	28	described	describe	VBN
cord-299281-5z1xminb	253	29	in	in	IN
cord-299281-5z1xminb	253	30	Materials	material	NNS
cord-299281-5z1xminb	253	31	and	and	CC
cord-299281-5z1xminb	253	32	Methods	method	NNS
cord-299281-5z1xminb	253	33	.	.	.
cord-299281-5z1xminb	254	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	254	2	were	be	VBD
cord-299281-5z1xminb	254	3	treated	treat	VBN
cord-299281-5z1xminb	254	4	with	with	IN
cord-299281-5z1xminb	254	5	TICK	tick	NN
cord-299281-5z1xminb	254	6	-	-	HYPH
cord-299281-5z1xminb	254	7	trypsin	trypsin	NN
cord-299281-5z1xminb	254	8	for	for	IN
cord-299281-5z1xminb	254	9	30	30	CD
cord-299281-5z1xminb	254	10	rain	rain	NN
cord-299281-5z1xminb	254	11	at	at	IN
cord-299281-5z1xminb	254	12	37	37	CD
cord-299281-5z1xminb	254	13	°	°	NN
cord-299281-5z1xminb	254	14	C	c	NN
cord-299281-5z1xminb	254	15	,	,	,
cord-299281-5z1xminb	254	16	and	and	CC
cord-299281-5z1xminb	254	17	then	then	RB
cord-299281-5z1xminb	254	18	solubilized	solubilize	VBD
cord-299281-5z1xminb	254	19	,	,	,
cord-299281-5z1xminb	254	20	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	254	21	with	with	IN
cord-299281-5z1xminb	254	22	anti	anti	JJ
cord-299281-5z1xminb	254	23	-	-	JJ
cord-299281-5z1xminb	254	24	VSV	VSV	NNP
cord-299281-5z1xminb	254	25	antibody	antibody	NN
cord-299281-5z1xminb	254	26	,	,	,
cord-299281-5z1xminb	254	27	and	and	CC
cord-299281-5z1xminb	254	28	analyzed	analyze	VBD
cord-299281-5z1xminb	254	29	on	on	IN
cord-299281-5z1xminb	254	30	SDS	SDS	NNP
cord-299281-5z1xminb	254	31	-	-	HYPH
cord-299281-5z1xminb	254	32	polyacrylamide	polyacrylamide	NN
cord-299281-5z1xminb	254	33	gels	gel	NNS
cord-299281-5z1xminb	254	34	(	(	-LRB-
cord-299281-5z1xminb	254	35	Fig	Fig	NNP
cord-299281-5z1xminb	254	36	.	.	.
cord-299281-5z1xminb	254	37	8	8	CD
cord-299281-5z1xminb	254	38	B	b	NN
cord-299281-5z1xminb	254	39	)	)	-RRB-
cord-299281-5z1xminb	254	40	.	.	.
cord-299281-5z1xminb	255	1	As	as	IN
cord-299281-5z1xminb	255	2	size	size	NN
cord-299281-5z1xminb	255	3	standards	standard	NNS
cord-299281-5z1xminb	255	4	we	-PRON-	PRP
cord-299281-5z1xminb	255	5	used	use	VBD
cord-299281-5z1xminb	255	6	radiolabeled	radiolabele	VBD
cord-299281-5z1xminb	255	7	tGml	tGml	NNP
cord-299281-5z1xminb	255	8	and	and	CC
cord-299281-5z1xminb	255	9	TMR	TMR	NNP
cord-299281-5z1xminb	255	10	immunoprecipitated	immunoprecipitate	VBD
cord-299281-5z1xminb	255	11	from	from	IN
cord-299281-5z1xminb	255	12	transfected	transfecte	VBN
cord-299281-5z1xminb	255	13	HeLa	HeLa	NNP
cord-299281-5z1xminb	255	14	cells	cell	NNS
cord-299281-5z1xminb	255	15	.	.	.
cord-299281-5z1xminb	256	1	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	256	2	cleaved	cleave	VBD
cord-299281-5z1xminb	256	3	newly	newly	RB
cord-299281-5z1xminb	256	4	synthesized	synthesize	VBN
cord-299281-5z1xminb	256	5	VSV	VSV	NNP
cord-299281-5z1xminb	256	6	G	G	NNP
cord-299281-5z1xminb	256	7	protein	protein	NN
cord-299281-5z1xminb	256	8	to	to	IN
cord-299281-5z1xminb	256	9	a	a	DT
cord-299281-5z1xminb	256	10	species	species	NN
cord-299281-5z1xminb	256	11	migrating	migrate	VBG
cord-299281-5z1xminb	256	12	slightly	slightly	RB
cord-299281-5z1xminb	256	13	larger	large	JJR
cord-299281-5z1xminb	256	14	than	than	IN
cord-299281-5z1xminb	256	15	TMR	TMR	NNP
cord-299281-5z1xminb	256	16	on	on	IN
cord-299281-5z1xminb	256	17	SDS	SDS	NNP
cord-299281-5z1xminb	256	18	-	-	HYPH
cord-299281-5z1xminb	256	19	PAGE	PAGE	NNP
cord-299281-5z1xminb	256	20	(	(	-LRB-
cord-299281-5z1xminb	256	21	Fig	Fig	NNP
cord-299281-5z1xminb	256	22	.	.	.
cord-299281-5z1xminb	256	23	8	8	CD
cord-299281-5z1xminb	256	24	B	b	NN
cord-299281-5z1xminb	256	25	,	,	,
cord-299281-5z1xminb	256	26	compare	compare	VB
cord-299281-5z1xminb	256	27	 	 	_SP
cord-299281-5z1xminb	256	28	lanes	lane	NNS
cord-299281-5z1xminb	256	29	7	7	CD
cord-299281-5z1xminb	256	30	and	and	CC
cord-299281-5z1xminb	256	31	10	10	CD
cord-299281-5z1xminb	256	32	)	)	-RRB-
cord-299281-5z1xminb	256	33	.	.	.
cord-299281-5z1xminb	257	1	After	after	IN
cord-299281-5z1xminb	257	2	60	60	CD
cord-299281-5z1xminb	257	3	rain	rain	NN
cord-299281-5z1xminb	257	4	of	of	IN
cord-299281-5z1xminb	257	5	chase	chase	NN
cord-299281-5z1xminb	257	6	,	,	,
cord-299281-5z1xminb	257	7	two	two	CD
cord-299281-5z1xminb	257	8	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	257	9	products	product	NNS
cord-299281-5z1xminb	257	10	were	be	VBD
cord-299281-5z1xminb	257	11	detected	detect	VBN
cord-299281-5z1xminb	257	12	.	.	.
cord-299281-5z1xminb	258	1	The	the	DT
cord-299281-5z1xminb	258	2	upper	upper	JJ
cord-299281-5z1xminb	258	3	band	band	NN
cord-299281-5z1xminb	258	4	corresponded	correspond	VBD
cord-299281-5z1xminb	258	5	to	to	IN
cord-299281-5z1xminb	258	6	cleavage	cleavage	NN
cord-299281-5z1xminb	258	7	of	of	IN
cord-299281-5z1xminb	258	8	the	the	DT
cord-299281-5z1xminb	258	9	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	258	10	tail	tail	NN
cord-299281-5z1xminb	258	11	of	of	IN
cord-299281-5z1xminb	258	12	mature	mature	JJ
cord-299281-5z1xminb	258	13	(	(	-LRB-
cord-299281-5z1xminb	258	14	sialylated	sialylated	JJ
cord-299281-5z1xminb	258	15	)	)	-RRB-
cord-299281-5z1xminb	258	16	VSV	VSV	NNP
cord-299281-5z1xminb	258	17	G	G	NNP
cord-299281-5z1xminb	258	18	,	,	,
cord-299281-5z1xminb	258	19	as	as	IN
cord-299281-5z1xminb	258	20	it	-PRON-	PRP
cord-299281-5z1xminb	258	21	was	be	VBD
cord-299281-5z1xminb	258	22	resistant	resistant	JJ
cord-299281-5z1xminb	258	23	to	to	IN
cord-299281-5z1xminb	258	24	endoglycosidase	endoglycosidase	NNP
cord-299281-5z1xminb	258	25	H	H	NNP
cord-299281-5z1xminb	258	26	,	,	,
cord-299281-5z1xminb	258	27	whereas	whereas	IN
cord-299281-5z1xminb	258	28	the	the	DT
cord-299281-5z1xminb	258	29	lower	low	JJR
cord-299281-5z1xminb	258	30	band	band	NN
cord-299281-5z1xminb	258	31	was	be	VBD
cord-299281-5z1xminb	258	32	sensitive	sensitive	JJ
cord-299281-5z1xminb	258	33	to	to	IN
cord-299281-5z1xminb	258	34	endoglycosidase	endoglycosidase	NNP
cord-299281-5z1xminb	258	35	H	h	NN
cord-299281-5z1xminb	258	36	treatment	treatment	NN
cord-299281-5z1xminb	258	37	(	(	-LRB-
cord-299281-5z1xminb	258	38	not	not	RB
cord-299281-5z1xminb	258	39	shown	show	VBN
cord-299281-5z1xminb	258	40	)	)	-RRB-
cord-299281-5z1xminb	258	41	.	.	.
cord-299281-5z1xminb	259	1	In	in	IN
cord-299281-5z1xminb	259	2	addition	addition	NN
cord-299281-5z1xminb	259	3	,	,	,
cord-299281-5z1xminb	259	4	we	-PRON-	PRP
cord-299281-5z1xminb	259	5	frequently	frequently	RB
cord-299281-5z1xminb	259	6	observed	observe	VBD
cord-299281-5z1xminb	259	7	some	some	DT
cord-299281-5z1xminb	259	8	residual	residual	JJ
cord-299281-5z1xminb	259	9	Gml	Gml	NNP
cord-299281-5z1xminb	259	10	or	or	CC
cord-299281-5z1xminb	259	11	VSV	VSV	NNP
cord-299281-5z1xminb	259	12	G	G	NNP
cord-299281-5z1xminb	259	13	were	be	VBD
cord-299281-5z1xminb	259	14	metabolically	metabolically	RB
cord-299281-5z1xminb	259	15	labeled	label	VBN
cord-299281-5z1xminb	259	16	for	for	IN
cord-299281-5z1xminb	259	17	5	5	CD
cord-299281-5z1xminb	259	18	rain	rain	NN
cord-299281-5z1xminb	259	19	,	,	,
cord-299281-5z1xminb	259	20	and	and	CC
cord-299281-5z1xminb	259	21	then	then	RB
cord-299281-5z1xminb	259	22	chased	chase	VBD
cord-299281-5z1xminb	259	23	for	for	IN
cord-299281-5z1xminb	259	24	0	0	CD
cord-299281-5z1xminb	259	25	or	or	CC
cord-299281-5z1xminb	259	26	60	60	CD
cord-299281-5z1xminb	259	27	rain	rain	NN
cord-299281-5z1xminb	259	28	.	.	.
cord-299281-5z1xminb	260	1	Microst	Microst	NNP
cord-299281-5z1xminb	260	2	,	,	,
cord-299281-5z1xminb	260	3	mes	mes	NNP
cord-299281-5z1xminb	260	4	were	be	VBD
cord-299281-5z1xminb	260	5	prepared	prepare	VBN
cord-299281-5z1xminb	260	6	as	as	IN
cord-299281-5z1xminb	260	7	described	describe	VBN
cord-299281-5z1xminb	260	8	in	in	IN
cord-299281-5z1xminb	260	9	Materials	material	NNS
cord-299281-5z1xminb	260	10	and	and	CC
cord-299281-5z1xminb	260	11	Methods	Methods	NNPS
cord-299281-5z1xminb	260	12	and	and	CC
cord-299281-5z1xminb	260	13	treated	treat	VBN
cord-299281-5z1xminb	260	14	with	with	IN
cord-299281-5z1xminb	260	15	20	20	CD
cord-299281-5z1xminb	260	16	#	#	$
cord-299281-5z1xminb	260	17	g	g	NN
cord-299281-5z1xminb	260	18	TPCK	TPCK	NNP
cord-299281-5z1xminb	260	19	-	-	HYPH
cord-299281-5z1xminb	260	20	trypsin	trypsin	NN
cord-299281-5z1xminb	260	21	(	(	-LRB-
cord-299281-5z1xminb	260	22	lanes	lane	NNS
cord-299281-5z1xminb	260	23	2	2	CD
cord-299281-5z1xminb	260	24	,	,	,
cord-299281-5z1xminb	260	25	4	4	CD
cord-299281-5z1xminb	260	26	,	,	,
cord-299281-5z1xminb	260	27	7	7	CD
cord-299281-5z1xminb	260	28	,	,	,
cord-299281-5z1xminb	260	29	and	and	CC
cord-299281-5z1xminb	260	30	9	9	CD
cord-299281-5z1xminb	260	31	)	)	-RRB-
cord-299281-5z1xminb	260	32	or	or	CC
cord-299281-5z1xminb	260	33	mock	mock	VB
cord-299281-5z1xminb	260	34	treated	treat	VBN
cord-299281-5z1xminb	260	35	(	(	-LRB-
cord-299281-5z1xminb	260	36	lanes	lane	NNS
cord-299281-5z1xminb	260	37	1	1	CD
cord-299281-5z1xminb	260	38	,	,	,
cord-299281-5z1xminb	260	39	3	3	CD
cord-299281-5z1xminb	260	40	,	,	,
cord-299281-5z1xminb	260	41	6	6	CD
cord-299281-5z1xminb	260	42	,	,	,
cord-299281-5z1xminb	260	43	and	and	CC
cord-299281-5z1xminb	260	44	8)	8)	NN
cord-299281-5z1xminb	260	45	for	for	IN
cord-299281-5z1xminb	260	46	30	30	CD
cord-299281-5z1xminb	260	47	min	min	NN
cord-299281-5z1xminb	260	48	at	at	IN
cord-299281-5z1xminb	260	49	37"C.	37"C.	NNP
cord-299281-5z1xminb	261	1	Proteolysis	Proteolysis	NNP
cord-299281-5z1xminb	261	2	was	be	VBD
cord-299281-5z1xminb	261	3	stopped	stop	VBN
cord-299281-5z1xminb	261	4	by	by	IN
cord-299281-5z1xminb	261	5	the	the	DT
cord-299281-5z1xminb	261	6	addition	addition	NN
cord-299281-5z1xminb	261	7	of	of	IN
cord-299281-5z1xminb	261	8	trypsin	trypsin	NN
cord-299281-5z1xminb	261	9	inhibitor	inhibitor	NN
cord-299281-5z1xminb	261	10	(	(	-LRB-
cord-299281-5z1xminb	261	11	20	20	CD
cord-299281-5z1xminb	261	12	/	/	SYM
cord-299281-5z1xminb	261	13	zg	zg	NNP
cord-299281-5z1xminb	261	14	)	)	-RRB-
cord-299281-5z1xminb	261	15	,	,	,
cord-299281-5z1xminb	261	16	the	the	DT
cord-299281-5z1xminb	261	17	microsomes	microsome	NNS
cord-299281-5z1xminb	261	18	were	be	VBD
cord-299281-5z1xminb	261	19	solubilized	solubilize	VBN
cord-299281-5z1xminb	261	20	,	,	,
cord-299281-5z1xminb	261	21	and	and	CC
cord-299281-5z1xminb	261	22	Gml	Gml	NNP
cord-299281-5z1xminb	261	23	(	(	-LRB-
cord-299281-5z1xminb	261	24	lanes	lane	NNS
cord-299281-5z1xminb	261	25	1	1	CD
cord-299281-5z1xminb	261	26	-	-	SYM
cord-299281-5z1xminb	261	27	4	4	CD
cord-299281-5z1xminb	261	28	)	)	-RRB-
cord-299281-5z1xminb	261	29	and	and	CC
cord-299281-5z1xminb	261	30	VSV	VSV	NNP
cord-299281-5z1xminb	261	31	G	G	NNP
cord-299281-5z1xminb	261	32	0anes	0anes	CD
cord-299281-5z1xminb	261	33	6	6	CD
cord-299281-5z1xminb	261	34	-	-	SYM
cord-299281-5z1xminb	261	35	9	9	CD
cord-299281-5z1xminb	261	36	)	)	-RRB-
cord-299281-5z1xminb	261	37	were	be	VBD
cord-299281-5z1xminb	261	38	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	261	39	and	and	CC
cord-299281-5z1xminb	261	40	analyzed	analyze	VBN
cord-299281-5z1xminb	261	41	by	by	IN
cord-299281-5z1xminb	261	42	SDS	SDS	NNP
cord-299281-5z1xminb	261	43	-	-	HYPH
cord-299281-5z1xminb	261	44	PAGE	PAGE	NNP
cord-299281-5z1xminb	261	45	.	.	.
cord-299281-5z1xminb	262	1	More	More	JJR
cord-299281-5z1xminb	262	2	inside	inside	IN
cord-299281-5z1xminb	262	3	-	-	HYPH
cord-299281-5z1xminb	262	4	out	out	RP
cord-299281-5z1xminb	262	5	microsomes	microsome	NNS
cord-299281-5z1xminb	262	6	are	be	VBP
cord-299281-5z1xminb	262	7	generated	generate	VBN
cord-299281-5z1xminb	262	8	from	from	IN
cord-299281-5z1xminb	262	9	compartments	compartment	NNS
cord-299281-5z1xminb	262	10	further	further	RB
cord-299281-5z1xminb	262	11	along	along	IN
cord-299281-5z1xminb	262	12	the	the	DT
cord-299281-5z1xminb	262	13	secretory	secretory	JJ
cord-299281-5z1xminb	262	14	pathway	pathway	NN
cord-299281-5z1xminb	262	15	,	,	,
cord-299281-5z1xminb	262	16	accounting	account	VBG
cord-299281-5z1xminb	262	17	for	for	IN
cord-299281-5z1xminb	262	18	the	the	DT
cord-299281-5z1xminb	262	19	loss	loss	NN
cord-299281-5z1xminb	262	20	of	of	IN
cord-299281-5z1xminb	262	21	material	material	NN
cord-299281-5z1xminb	262	22	recovered	recover	VBN
cord-299281-5z1xminb	262	23	after	after	IN
cord-299281-5z1xminb	262	24	trypsinization	trypsinization	NN
cord-299281-5z1xminb	262	25	of	of	IN
cord-299281-5z1xminb	262	26	VSV	VSV	NNP
cord-299281-5z1xminb	262	27	G	G	NNP
cord-299281-5z1xminb	262	28	isolated	isolate	VBN
cord-299281-5z1xminb	262	29	after	after	IN
cord-299281-5z1xminb	262	30	a	a	DT
cord-299281-5z1xminb	262	31	60-min	60-min	CD
cord-299281-5z1xminb	262	32	chase	chase	NN
cord-299281-5z1xminb	262	33	(	(	-LRB-
cord-299281-5z1xminb	262	34	lane	lane	NN
cord-299281-5z1xminb	262	35	9	9	CD
cord-299281-5z1xminb	262	36	)	)	-RRB-
cord-299281-5z1xminb	262	37	.	.	.
cord-299281-5z1xminb	263	1	tGml	tGml	NNP
cord-299281-5z1xminb	263	2	(	(	-LRB-
cord-299281-5z1xminb	263	3	lane	lane	NN
cord-299281-5z1xminb	263	4	5	5	CD
cord-299281-5z1xminb	263	5	)	)	-RRB-
cord-299281-5z1xminb	263	6	and	and	CC
cord-299281-5z1xminb	263	7	TMR	TMR	NNP
cord-299281-5z1xminb	263	8	(	(	-LRB-
cord-299281-5z1xminb	263	9	lane	lane	NNP
cord-299281-5z1xminb	263	10	10	10	CD
cord-299281-5z1xminb	263	11	)	)	-RRB-
cord-299281-5z1xminb	263	12	immunoprecipitated	immunoprecipitate	VBD
cord-299281-5z1xminb	263	13	from	from	IN
cord-299281-5z1xminb	263	14	HeLa	HeLa	NNP
cord-299281-5z1xminb	263	15	cells	cell	NNS
cord-299281-5z1xminb	263	16	labeled	label	VBN
cord-299281-5z1xminb	263	17	under	under	IN
cord-299281-5z1xminb	263	18	the	the	DT
cord-299281-5z1xminb	263	19	same	same	JJ
cord-299281-5z1xminb	263	20	conditions	condition	NNS
cord-299281-5z1xminb	263	21	are	be	VBP
cord-299281-5z1xminb	263	22	included	include	VBN
cord-299281-5z1xminb	263	23	as	as	IN
cord-299281-5z1xminb	263	24	size	size	NN
cord-299281-5z1xminb	263	25	standards	standard	NNS
cord-299281-5z1xminb	263	26	.	.	.
cord-299281-5z1xminb	264	1	uncleaved	uncleave	VBN
cord-299281-5z1xminb	264	2	mature	mature	JJ
cord-299281-5z1xminb	264	3	VSV	VSV	NNP
cord-299281-5z1xminb	264	4	G	G	NNP
cord-299281-5z1xminb	264	5	in	in	IN
cord-299281-5z1xminb	264	6	our	-PRON-	PRP$
cord-299281-5z1xminb	264	7	experiments	experiment	NNS
cord-299281-5z1xminb	264	8	.	.	.
cord-299281-5z1xminb	265	1	Since	since	IN
cord-299281-5z1xminb	265	2	the	the	DT
cord-299281-5z1xminb	265	3	trypsin	trypsin	NN
cord-299281-5z1xminb	265	4	-	-	HYPH
cord-299281-5z1xminb	265	5	cleaved	cleave	VBN
cord-299281-5z1xminb	265	6	form	form	NN
cord-299281-5z1xminb	265	7	of	of	IN
cord-299281-5z1xminb	265	8	VSV	VSV	NNP
cord-299281-5z1xminb	265	9	G	G	NNP
cord-299281-5z1xminb	265	10	migrated	migrate	VBD
cord-299281-5z1xminb	265	11	more	more	RBR
cord-299281-5z1xminb	265	12	slowly	slowly	RB
cord-299281-5z1xminb	265	13	than	than	IN
cord-299281-5z1xminb	265	14	T	T	NNP
cord-299281-5z1xminb	265	15	M	m	NN
cord-299281-5z1xminb	265	16	R	r	NN
cord-299281-5z1xminb	265	17	(	(	-LRB-
cord-299281-5z1xminb	265	18	whose	whose	WP$
cord-299281-5z1xminb	265	19	tail	tail	NN
cord-299281-5z1xminb	265	20	contains	contain	VBZ
cord-299281-5z1xminb	265	21	a	a	DT
cord-299281-5z1xminb	265	22	single	single	JJ
cord-299281-5z1xminb	265	23	amino	amino	NN
cord-299281-5z1xminb	265	24	acid	acid	NN
cord-299281-5z1xminb	265	25	)	)	-RRB-
cord-299281-5z1xminb	265	26	,	,	,
cord-299281-5z1xminb	265	27	we	-PRON-	PRP
cord-299281-5z1xminb	265	28	deduced	deduce	VBD
cord-299281-5z1xminb	265	29	that	that	IN
cord-299281-5z1xminb	265	30	trypsin	trypsin	NN
cord-299281-5z1xminb	265	31	cleaves	cleave	VBZ
cord-299281-5z1xminb	265	32	the	the	DT
cord-299281-5z1xminb	265	33	VSV	VSV	NNP
cord-299281-5z1xminb	265	34	G	G	NNP
cord-299281-5z1xminb	265	35	tall	tall	JJ
cord-299281-5z1xminb	265	36	within	within	IN
cord-299281-5z1xminb	265	37	region	region	NN
cord-299281-5z1xminb	266	1	A	a	DT
cord-299281-5z1xminb	266	2	marked	marked	JJ
cord-299281-5z1xminb	266	3	on	on	IN
cord-299281-5z1xminb	266	4	Fig	Fig	NNP
cord-299281-5z1xminb	266	5	.	.	.
cord-299281-5z1xminb	267	1	8	8	CD
cord-299281-5z1xminb	267	2	A.	a.	NN
cord-299281-5z1xminb	268	1	The	the	DT
cord-299281-5z1xminb	268	2	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	268	3	profile	profile	NN
cord-299281-5z1xminb	268	4	generated	generate	VBN
cord-299281-5z1xminb	268	5	by	by	IN
cord-299281-5z1xminb	268	6	trypsin	trypsin	NN
cord-299281-5z1xminb	268	7	cleavage	cleavage	NN
cord-299281-5z1xminb	268	8	of	of	IN
cord-299281-5z1xminb	268	9	Gml	Gml	NNP
cord-299281-5z1xminb	268	10	differed	differ	VBD
cord-299281-5z1xminb	268	11	from	from	IN
cord-299281-5z1xminb	268	12	that	that	DT
cord-299281-5z1xminb	268	13	of	of	IN
cord-299281-5z1xminb	268	14	VSV	VSV	NNP
cord-299281-5z1xminb	268	15	G.	G.	NNP
cord-299281-5z1xminb	268	16	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	268	17	treatment	treatment	NN
cord-299281-5z1xminb	268	18	of	of	IN
cord-299281-5z1xminb	268	19	Figure	figure	NN
cord-299281-5z1xminb	268	20	9	9	CD
cord-299281-5z1xminb	268	21	.	.	.
cord-299281-5z1xminb	269	1	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	269	2	-	-	HYPH
cord-299281-5z1xminb	269	3	treated	treat	VBN
cord-299281-5z1xminb	269	4	Gml	Gml	NNP
cord-299281-5z1xminb	269	5	is	be	VBZ
cord-299281-5z1xminb	269	6	still	still	RB
cord-299281-5z1xminb	269	7	oligomerized	oligomerize	VBN
cord-299281-5z1xminb	269	8	.	.	.
cord-299281-5z1xminb	270	1	Cells	cell	NNS
cord-299281-5z1xminb	270	2	expressing	express	VBG
cord-299281-5z1xminb	270	3	VSV	VSV	NNP
cord-299281-5z1xminb	270	4	G	G	NNP
cord-299281-5z1xminb	270	5	(	(	-LRB-
cord-299281-5z1xminb	270	6	A	a	NN
cord-299281-5z1xminb	270	7	)	)	-RRB-
cord-299281-5z1xminb	270	8	or	or	CC
cord-299281-5z1xminb	270	9	Gml	Gml	NNP
cord-299281-5z1xminb	270	10	(	(	-LRB-
cord-299281-5z1xminb	270	11	B	b	NN
cord-299281-5z1xminb	270	12	)	)	-RRB-
cord-299281-5z1xminb	270	13	were	be	VBD
cord-299281-5z1xminb	270	14	metabolically	metabolically	RB
cord-299281-5z1xminb	270	15	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	270	16	for	for	IN
cord-299281-5z1xminb	270	17	5	5	CD
cord-299281-5z1xminb	270	18	rain	rain	NN
cord-299281-5z1xminb	270	19	,	,	,
cord-299281-5z1xminb	270	20	and	and	CC
cord-299281-5z1xminb	270	21	then	then	RB
cord-299281-5z1xminb	270	22	chased	chase	VBD
cord-299281-5z1xminb	270	23	for	for	IN
cord-299281-5z1xminb	270	24	45	45	CD
cord-299281-5z1xminb	270	25	rain	rain	NN
cord-299281-5z1xminb	270	26	.	.	.
cord-299281-5z1xminb	271	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	271	2	were	be	VBD
cord-299281-5z1xminb	271	3	prepared	prepare	VBN
cord-299281-5z1xminb	271	4	and	and	CC
cord-299281-5z1xminb	271	5	treated	treat	VBN
cord-299281-5z1xminb	271	6	with	with	IN
cord-299281-5z1xminb	271	7	20	20	CD
cord-299281-5z1xminb	271	8	/	/	SYM
cord-299281-5z1xminb	271	9	zg	zg	CC
cord-299281-5z1xminb	271	10	TICK	tick	NN
cord-299281-5z1xminb	271	11	-	-	HYPH
cord-299281-5z1xminb	271	12	trypsin	trypsin	VBP
cord-299281-5z1xminb	271	13	for	for	IN
cord-299281-5z1xminb	271	14	30	30	CD
cord-299281-5z1xminb	271	15	rain	rain	NN
cord-299281-5z1xminb	271	16	at	at	IN
cord-299281-5z1xminb	271	17	37	37	CD
cord-299281-5z1xminb	271	18	°	°	NN
cord-299281-5z1xminb	271	19	C	c	NN
cord-299281-5z1xminb	271	20	,	,	,
cord-299281-5z1xminb	271	21	then	then	RB
cord-299281-5z1xminb	271	22	solubilized	solubilize	VBD
cord-299281-5z1xminb	271	23	in	in	IN
cord-299281-5z1xminb	271	24	MNT	MNT	NNP
cord-299281-5z1xminb	271	25	and	and	CC
cord-299281-5z1xminb	271	26	centrifuged	centrifuge	VBD
cord-299281-5z1xminb	271	27	on	on	IN
cord-299281-5z1xminb	271	28	5	5	CD
cord-299281-5z1xminb	271	29	-	-	SYM
cord-299281-5z1xminb	271	30	20	20	CD
cord-299281-5z1xminb	271	31	%	%	NN
cord-299281-5z1xminb	271	32	sucrose	sucrose	NN
cord-299281-5z1xminb	271	33	gradients	gradient	NNS
cord-299281-5z1xminb	271	34	.	.	.
cord-299281-5z1xminb	272	1	Fractions	fraction	NNS
cord-299281-5z1xminb	272	2	were	be	VBD
cord-299281-5z1xminb	272	3	collected	collect	VBN
cord-299281-5z1xminb	272	4	,	,	,
cord-299281-5z1xminb	272	5	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	272	6	using	use	VBG
cord-299281-5z1xminb	272	7	anti	anti	JJ
cord-299281-5z1xminb	272	8	-	-	JJ
cord-299281-5z1xminb	272	9	VSV	VSV	NNP
cord-299281-5z1xminb	272	10	antibody	antibody	NN
cord-299281-5z1xminb	272	11	,	,	,
cord-299281-5z1xminb	272	12	and	and	CC
cord-299281-5z1xminb	272	13	analyzed	analyze	VBN
cord-299281-5z1xminb	272	14	by	by	IN
cord-299281-5z1xminb	272	15	SDS	SDS	NNP
cord-299281-5z1xminb	272	16	-	-	HYPH
cord-299281-5z1xminb	272	17	PAGE	PAGE	NNP
cord-299281-5z1xminb	272	18	.	.	.
cord-299281-5z1xminb	273	1	newly	newly	RB
cord-299281-5z1xminb	273	2	synthesized	synthesize	VBN
cord-299281-5z1xminb	273	3	Gml	Gml	NNP
cord-299281-5z1xminb	273	4	yielded	yield	VBD
cord-299281-5z1xminb	273	5	a	a	DT
cord-299281-5z1xminb	273	6	single	single	JJ
cord-299281-5z1xminb	273	7	species	specie	NNS
cord-299281-5z1xminb	273	8	which	which	WDT
cord-299281-5z1xminb	273	9	migrated	migrate	VBD
cord-299281-5z1xminb	273	10	more	more	RBR
cord-299281-5z1xminb	273	11	slowly	slowly	RB
cord-299281-5z1xminb	273	12	on	on	IN
cord-299281-5z1xminb	273	13	SDS	SDS	NNP
cord-299281-5z1xminb	273	14	-	-	HYPH
cord-299281-5z1xminb	273	15	PAGE	PAGE	NNP
cord-299281-5z1xminb	273	16	than	than	IN
cord-299281-5z1xminb	273	17	the	the	DT
cord-299281-5z1xminb	273	18	VSV	VSV	NNP
cord-299281-5z1xminb	273	19	G	G	NNP
cord-299281-5z1xminb	273	20	proteolyric	proteolyric	NN
cord-299281-5z1xminb	273	21	fragment	fragment	NN
cord-299281-5z1xminb	273	22	.	.	.
cord-299281-5z1xminb	274	1	Thus	thus	RB
cord-299281-5z1xminb	274	2	,	,	,
cord-299281-5z1xminb	274	3	even	even	RB
cord-299281-5z1xminb	274	4	before	before	IN
cord-299281-5z1xminb	274	5	oligomers	oligomer	NNS
cord-299281-5z1xminb	274	6	are	be	VBP
cord-299281-5z1xminb	274	7	formed	form	VBN
cord-299281-5z1xminb	274	8	,	,	,
cord-299281-5z1xminb	274	9	the	the	DT
cord-299281-5z1xminb	274	10	tail	tail	NN
cord-299281-5z1xminb	274	11	of	of	IN
cord-299281-5z1xminb	274	12	Gml	Gml	NNP
cord-299281-5z1xminb	274	13	is	be	VBZ
cord-299281-5z1xminb	274	14	less	less	RBR
cord-299281-5z1xminb	274	15	accessible	accessible	JJ
cord-299281-5z1xminb	274	16	to	to	IN
cord-299281-5z1xminb	274	17	trypsin	trypsin	NN
cord-299281-5z1xminb	274	18	than	than	IN
cord-299281-5z1xminb	274	19	that	that	DT
cord-299281-5z1xminb	274	20	of	of	IN
cord-299281-5z1xminb	274	21	VSV	VSV	NNP
cord-299281-5z1xminb	274	22	G.	G.	NNP
cord-299281-5z1xminb	275	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	275	2	,	,	,
cord-299281-5z1xminb	275	3	when	when	WRB
cord-299281-5z1xminb	275	4	Gml	Gml	NNP
cord-299281-5z1xminb	275	5	was	be	VBD
cord-299281-5z1xminb	275	6	digested	digest	VBN
cord-299281-5z1xminb	275	7	after	after	IN
cord-299281-5z1xminb	275	8	a	a	DT
cord-299281-5z1xminb	275	9	60-rain	60-rain	CD
cord-299281-5z1xminb	275	10	chase	chase	NN
cord-299281-5z1xminb	275	11	,	,	,
cord-299281-5z1xminb	275	12	there	there	EX
cord-299281-5z1xminb	275	13	was	be	VBD
cord-299281-5z1xminb	275	14	a	a	DT
cord-299281-5z1xminb	275	15	large	large	JJ
cord-299281-5z1xminb	275	16	reduction	reduction	NN
cord-299281-5z1xminb	275	17	in	in	IN
cord-299281-5z1xminb	275	18	the	the	DT
cord-299281-5z1xminb	275	19	amount	amount	NN
cord-299281-5z1xminb	275	20	of	of	IN
cord-299281-5z1xminb	275	21	SDS	SDS	NNP
cord-299281-5z1xminb	275	22	-	-	HYPH
cord-299281-5z1xminb	275	23	resistant	resistant	JJ
cord-299281-5z1xminb	275	24	oligomer	oligomer	NNP
cord-299281-5z1xminb	275	25	migrating	migrate	VBG
cord-299281-5z1xminb	275	26	at	at	IN
cord-299281-5z1xminb	275	27	the	the	DT
cord-299281-5z1xminb	275	28	top	top	NN
cord-299281-5z1xminb	275	29	of	of	IN
cord-299281-5z1xminb	275	30	the	the	DT
cord-299281-5z1xminb	275	31	stacking	stacking	NN
cord-299281-5z1xminb	275	32	gel	gel	NN
cord-299281-5z1xminb	275	33	on	on	IN
cord-299281-5z1xminb	275	34	SDS	SDS	NNP
cord-299281-5z1xminb	275	35	-	-	HYPH
cord-299281-5z1xminb	275	36	PAGE	PAGE	NNP
cord-299281-5z1xminb	275	37	(	(	-LRB-
cord-299281-5z1xminb	275	38	Fig	Fig	NNP
cord-299281-5z1xminb	275	39	.	.	.
cord-299281-5z1xminb	275	40	8	8	CD
cord-299281-5z1xminb	275	41	B	b	NN
cord-299281-5z1xminb	275	42	,	,	,
cord-299281-5z1xminb	275	43	compare	compare	VB
cord-299281-5z1xminb	275	44	lanes	lane	NNS
cord-299281-5z1xminb	275	45	3	3	CD
cord-299281-5z1xminb	275	46	and	and	CC
cord-299281-5z1xminb	275	47	4	4	CD
cord-299281-5z1xminb	275	48	)	)	-RRB-
cord-299281-5z1xminb	275	49	.	.	.
cord-299281-5z1xminb	276	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	276	2	,	,	,
cord-299281-5z1xminb	276	3	we	-PRON-	PRP
cord-299281-5z1xminb	276	4	saw	see	VBD
cord-299281-5z1xminb	276	5	a	a	DT
cord-299281-5z1xminb	276	6	second	second	JJ
cord-299281-5z1xminb	276	7	,	,	,
cord-299281-5z1xminb	276	8	larger	large	JJR
cord-299281-5z1xminb	276	9	band	band	NN
cord-299281-5z1xminb	276	10	in	in	IN
cord-299281-5z1xminb	276	11	addition	addition	NN
cord-299281-5z1xminb	276	12	to	to	IN
cord-299281-5z1xminb	276	13	the	the	DT
cord-299281-5z1xminb	276	14	one	one	NN
cord-299281-5z1xminb	276	15	detected	detect	VBN
cord-299281-5z1xminb	276	16	immediately	immediately	RB
cord-299281-5z1xminb	276	17	after	after	IN
cord-299281-5z1xminb	276	18	the	the	DT
cord-299281-5z1xminb	276	19	pulse	pulse	NN
cord-299281-5z1xminb	276	20	label	label	NN
cord-299281-5z1xminb	276	21	.	.	.
cord-299281-5z1xminb	277	1	Both	both	DT
cord-299281-5z1xminb	277	2	bands	band	NNS
cord-299281-5z1xminb	277	3	were	be	VBD
cord-299281-5z1xminb	277	4	endoglycosidase	endoglycosidase	NNP
cord-299281-5z1xminb	277	5	H	H	NNP
cord-299281-5z1xminb	277	6	-	-	HYPH
cord-299281-5z1xminb	277	7	sensitive	sensitive	JJ
cord-299281-5z1xminb	277	8	,	,	,
cord-299281-5z1xminb	277	9	indicating	indicate	VBG
cord-299281-5z1xminb	277	10	that	that	IN
cord-299281-5z1xminb	277	11	the	the	DT
cord-299281-5z1xminb	277	12	proteins	protein	NNS
cord-299281-5z1xminb	277	13	they	-PRON-	PRP
cord-299281-5z1xminb	277	14	represent	represent	VBP
cord-299281-5z1xminb	277	15	had	have	VBD
cord-299281-5z1xminb	277	16	not	not	RB
cord-299281-5z1xminb	277	17	passed	pass	VBN
cord-299281-5z1xminb	277	18	through	through	IN
cord-299281-5z1xminb	277	19	the	the	DT
cord-299281-5z1xminb	277	20	medial	medial	JJ
cord-299281-5z1xminb	277	21	Golgi	Golgi	NNP
cord-299281-5z1xminb	277	22	(	(	-LRB-
cord-299281-5z1xminb	277	23	not	not	RB
cord-299281-5z1xminb	277	24	shown	show	VBN
cord-299281-5z1xminb	277	25	)	)	-RRB-
cord-299281-5z1xminb	277	26	.	.	.
cord-299281-5z1xminb	278	1	In	in	IN
cord-299281-5z1xminb	278	2	more	more	RBR
cord-299281-5z1xminb	278	3	detailed	detailed	JJ
cord-299281-5z1xminb	278	4	kinetic	kinetic	JJ
cord-299281-5z1xminb	278	5	experiments	experiment	NNS
cord-299281-5z1xminb	278	6	,	,	,
cord-299281-5z1xminb	278	7	the	the	DT
cord-299281-5z1xminb	278	8	amount	amount	NN
cord-299281-5z1xminb	278	9	of	of	IN
cord-299281-5z1xminb	278	10	this	this	DT
cord-299281-5z1xminb	278	11	upper	upper	JJ
cord-299281-5z1xminb	278	12	band	band	NN
cord-299281-5z1xminb	278	13	increased	increase	VBD
cord-299281-5z1xminb	278	14	with	with	IN
cord-299281-5z1xminb	278	15	longer	long	JJR
cord-299281-5z1xminb	278	16	chase	chase	NNP
cord-299281-5z1xminb	278	17	times	time	NNS
cord-299281-5z1xminb	278	18	,	,	,
cord-299281-5z1xminb	278	19	with	with	IN
cord-299281-5z1xminb	278	20	a	a	DT
cord-299281-5z1xminb	278	21	concomitant	concomitant	JJ
cord-299281-5z1xminb	278	22	decrease	decrease	NN
cord-299281-5z1xminb	278	23	in	in	IN
cord-299281-5z1xminb	278	24	the	the	DT
cord-299281-5z1xminb	278	25	amount	amount	NN
cord-299281-5z1xminb	278	26	of	of	IN
cord-299281-5z1xminb	278	27	the	the	DT
cord-299281-5z1xminb	278	28	lower	low	JJR
cord-299281-5z1xminb	278	29	band	band	NN
cord-299281-5z1xminb	278	30	(	(	-LRB-
cord-299281-5z1xminb	278	31	not	not	RB
cord-299281-5z1xminb	278	32	shown	show	VBN
cord-299281-5z1xminb	278	33	)	)	-RRB-
cord-299281-5z1xminb	278	34	.	.	.
cord-299281-5z1xminb	279	1	Based	base	VBN
cord-299281-5z1xminb	279	2	on	on	IN
cord-299281-5z1xminb	279	3	the	the	DT
cord-299281-5z1xminb	279	4	potential	potential	JJ
cord-299281-5z1xminb	279	5	trypsin	trypsin	NN
cord-299281-5z1xminb	279	6	cleavage	cleavage	NN
cord-299281-5z1xminb	279	7	sites	site	NNS
cord-299281-5z1xminb	279	8	in	in	IN
cord-299281-5z1xminb	279	9	the	the	DT
cord-299281-5z1xminb	279	10	Figure	Figure	NNP
cord-299281-5z1xminb	279	11	10	10	CD
cord-299281-5z1xminb	279	12	.	.	.
cord-299281-5z1xminb	280	1	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	280	2	treatment	treatment	NN
cord-299281-5z1xminb	280	3	of	of	IN
cord-299281-5z1xminb	280	4	Gml	Gml	NNP
cord-299281-5z1xminb	280	5	and	and	CC
cord-299281-5z1xminb	280	6	VSV	VSV	NNP
cord-299281-5z1xminb	280	7	G	G	NNP
cord-299281-5z1xminb	280	8	recovered	recover	VBD
cord-299281-5z1xminb	280	9	after	after	IN
cord-299281-5z1xminb	280	10	treatment	treatment	NN
cord-299281-5z1xminb	280	11	with	with	IN
cord-299281-5z1xminb	280	12	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	280	13	D.	D.	NNP
cord-299281-5z1xminb	280	14	HeLa	HeLa	NNP
cord-299281-5z1xminb	280	15	cells	cell	NNS
cord-299281-5z1xminb	280	16	expressing	express	VBG
cord-299281-5z1xminb	280	17	Gml	Gml	NNP
cord-299281-5z1xminb	280	18	or	or	CC
cord-299281-5z1xminb	280	19	VSV	VSV	NNP
cord-299281-5z1xminb	280	20	G	G	NNP
cord-299281-5z1xminb	280	21	were	be	VBD
cord-299281-5z1xminb	280	22	treated	treat	VBN
cord-299281-5z1xminb	280	23	with	with	IN
cord-299281-5z1xminb	280	24	100	100	CD
cord-299281-5z1xminb	280	25	#	#	NN
cord-299281-5z1xminb	280	26	M	m	NN
cord-299281-5z1xminb	280	27	cytochalasin	cytochalasin	FW
cord-299281-5z1xminb	280	28	D	d	NN
cord-299281-5z1xminb	280	29	starting	start	VBG
cord-299281-5z1xminb	280	30	1	1	CD
cord-299281-5z1xminb	280	31	h	h	NN
cord-299281-5z1xminb	280	32	before	before	IN
cord-299281-5z1xminb	280	33	radiolabeling	radiolabele	VBG
cord-299281-5z1xminb	280	34	.	.	.
cord-299281-5z1xminb	281	1	Because	because	IN
cord-299281-5z1xminb	281	2	fewer	few	JJR
cord-299281-5z1xminb	281	3	microsomes	microsome	NNS
cord-299281-5z1xminb	281	4	were	be	VBD
cord-299281-5z1xminb	281	5	recovered	recover	VBN
cord-299281-5z1xminb	281	6	from	from	IN
cord-299281-5z1xminb	281	7	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	281	8	D	D	NNP
cord-299281-5z1xminb	281	9	-	-	HYPH
cord-299281-5z1xminb	281	10	treated	treat	VBN
cord-299281-5z1xminb	281	11	cells	cell	NNS
cord-299281-5z1xminb	281	12	than	than	IN
cord-299281-5z1xminb	281	13	from	from	IN
cord-299281-5z1xminb	281	14	untreated	untreated	JJ
cord-299281-5z1xminb	281	15	ceils	ceil	NNS
cord-299281-5z1xminb	281	16	,	,	,
cord-299281-5z1xminb	281	17	treated	treat	VBN
cord-299281-5z1xminb	281	18	cells	cell	NNS
cord-299281-5z1xminb	281	19	were	be	VBD
cord-299281-5z1xminb	281	20	radiolabeled	radiolabele	VBN
cord-299281-5z1xminb	281	21	with	with	IN
cord-299281-5z1xminb	281	22	twice	twice	PDT
cord-299281-5z1xminb	281	23	the	the	DT
cord-299281-5z1xminb	281	24	usual	usual	JJ
cord-299281-5z1xminb	281	25	concentration	concentration	NN
cord-299281-5z1xminb	281	26	of	of	IN
cord-299281-5z1xminb	281	27	radioactivity	radioactivity	NN
cord-299281-5z1xminb	281	28	.	.	.
cord-299281-5z1xminb	282	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	282	2	were	be	VBD
cord-299281-5z1xminb	282	3	prepared	prepare	VBN
cord-299281-5z1xminb	282	4	after	after	IN
cord-299281-5z1xminb	282	5	a	a	DT
cord-299281-5z1xminb	282	6	15-min	15-min	CD
cord-299281-5z1xminb	282	7	pulse	pulse	NN
cord-299281-5z1xminb	282	8	and	and	CC
cord-299281-5z1xminb	282	9	a	a	DT
cord-299281-5z1xminb	282	10	45-min	45-min	CD
cord-299281-5z1xminb	282	11	chase	chase	NN
cord-299281-5z1xminb	282	12	,	,	,
cord-299281-5z1xminb	282	13	and	and	CC
cord-299281-5z1xminb	282	14	aliquots	aliquot	NNS
cord-299281-5z1xminb	282	15	were	be	VBD
cord-299281-5z1xminb	282	16	mock	mock	JJ
cord-299281-5z1xminb	282	17	treated	treat	VBN
cord-299281-5z1xminb	282	18	or	or	CC
cord-299281-5z1xminb	282	19	treated	treat	VBN
cord-299281-5z1xminb	282	20	with	with	IN
cord-299281-5z1xminb	282	21	TPCK	TPCK	NNP
cord-299281-5z1xminb	282	22	-	-	HYPH
cord-299281-5z1xminb	282	23	trypsin	trypsin	NN
cord-299281-5z1xminb	282	24	as	as	IN
cord-299281-5z1xminb	282	25	described	describe	VBN
cord-299281-5z1xminb	282	26	in	in	IN
cord-299281-5z1xminb	282	27	Materials	material	NNS
cord-299281-5z1xminb	282	28	and	and	CC
cord-299281-5z1xminb	282	29	Methods	method	NNS
cord-299281-5z1xminb	282	30	.	.	.
cord-299281-5z1xminb	283	1	Gml	Gml	NNP
cord-299281-5z1xminb	283	2	and	and	CC
cord-299281-5z1xminb	283	3	VSV	VSV	NNP
cord-299281-5z1xminb	283	4	G	G	NNP
cord-299281-5z1xminb	283	5	were	be	VBD
cord-299281-5z1xminb	283	6	immunoprecipitated	immunoprecipitate	VBN
cord-299281-5z1xminb	283	7	using	use	VBG
cord-299281-5z1xminb	283	8	anti	anti	JJ
cord-299281-5z1xminb	283	9	-	-	JJ
cord-299281-5z1xminb	283	10	VSV	VSV	NNP
cord-299281-5z1xminb	283	11	antibody	antibody	NN
cord-299281-5z1xminb	283	12	and	and	CC
cord-299281-5z1xminb	283	13	analyzed	analyze	VBN
cord-299281-5z1xminb	283	14	by	by	IN
cord-299281-5z1xminb	283	15	SDS	SDS	NNP
cord-299281-5z1xminb	283	16	-	-	HYPH
cord-299281-5z1xminb	283	17	PAGE	PAGE	NNP
cord-299281-5z1xminb	283	18	.	.	.
cord-299281-5z1xminb	284	1	Cytochalasin	Cytochalasin	NNP
cord-299281-5z1xminb	284	2	D	D	NNP
cord-299281-5z1xminb	284	3	blocked	block	VBD
cord-299281-5z1xminb	284	4	formation	formation	NN
cord-299281-5z1xminb	284	5	of	of	IN
cord-299281-5z1xminb	284	6	SDS	sds	NN
cord-299281-5z1xminb	284	7	-	-	HYPH
cord-299281-5z1xminb	284	8	resistant	resistant	JJ
cord-299281-5z1xminb	284	9	oligomers	oligomer	NNS
cord-299281-5z1xminb	284	10	and	and	CC
cord-299281-5z1xminb	284	11	accumulation	accumulation	NN
cord-299281-5z1xminb	284	12	of	of	IN
cord-299281-5z1xminb	284	13	the	the	DT
cord-299281-5z1xminb	284	14	larger	large	JJR
cord-299281-5z1xminb	284	15	trypsin	trypsin	NN
cord-299281-5z1xminb	284	16	-	-	HYPH
cord-299281-5z1xminb	284	17	cleaved	cleave	VBN
cord-299281-5z1xminb	284	18	band	band	NN
cord-299281-5z1xminb	284	19	.	.	.
cord-299281-5z1xminb	285	1	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	285	2	tail	tail	NN
cord-299281-5z1xminb	285	3	and	and	CC
cord-299281-5z1xminb	285	4	the	the	DT
cord-299281-5z1xminb	285	5	relative	relative	JJ
cord-299281-5z1xminb	285	6	mobility	mobility	NN
cord-299281-5z1xminb	285	7	of	of	IN
cord-299281-5z1xminb	285	8	these	these	DT
cord-299281-5z1xminb	285	9	bands	band	NNS
cord-299281-5z1xminb	286	1	compared	compare	VBN
cord-299281-5z1xminb	286	2	to	to	IN
cord-299281-5z1xminb	286	3	the	the	DT
cord-299281-5z1xminb	286	4	VSV	VSV	NNP
cord-299281-5z1xminb	286	5	G	G	NNP
cord-299281-5z1xminb	286	6	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	286	7	fragment	fragment	NN
cord-299281-5z1xminb	286	8	and	and	CC
cord-299281-5z1xminb	286	9	tGml	tGml	NNP
cord-299281-5z1xminb	286	10	,	,	,
cord-299281-5z1xminb	286	11	we	-PRON-	PRP
cord-299281-5z1xminb	286	12	conclude	conclude	VBP
cord-299281-5z1xminb	286	13	that	that	IN
cord-299281-5z1xminb	286	14	the	the	DT
cord-299281-5z1xminb	286	15	upper	upper	JJ
cord-299281-5z1xminb	286	16	and	and	CC
cord-299281-5z1xminb	286	17	lower	low	JJR
cord-299281-5z1xminb	286	18	bands	band	NNS
cord-299281-5z1xminb	286	19	result	result	VBP
cord-299281-5z1xminb	286	20	from	from	IN
cord-299281-5z1xminb	286	21	trypsin	trypsin	NN
cord-299281-5z1xminb	286	22	cleavage	cleavage	NN
cord-299281-5z1xminb	286	23	within	within	IN
cord-299281-5z1xminb	286	24	regions	region	NNS
cord-299281-5z1xminb	286	25	C	c	NN
cord-299281-5z1xminb	286	26	and	and	CC
cord-299281-5z1xminb	286	27	B	B	NNP
cord-299281-5z1xminb	286	28	,	,	,
cord-299281-5z1xminb	286	29	respectively	respectively	RB
cord-299281-5z1xminb	286	30	(	(	-LRB-
cord-299281-5z1xminb	286	31	Fig	Fig	NNP
cord-299281-5z1xminb	286	32	.	.	.
cord-299281-5z1xminb	286	33	8	8	CD
cord-299281-5z1xminb	286	34	A	a	NN
cord-299281-5z1xminb	286	35	)	)	-RRB-
cord-299281-5z1xminb	286	36	.	.	.
cord-299281-5z1xminb	287	1	The	the	DT
cord-299281-5z1xminb	287	2	upper	upper	JJ
cord-299281-5z1xminb	287	3	band	band	NN
cord-299281-5z1xminb	287	4	may	may	MD
cord-299281-5z1xminb	287	5	represent	represent	VB
cord-299281-5z1xminb	287	6	material	material	NN
cord-299281-5z1xminb	287	7	generated	generate	VBN
cord-299281-5z1xminb	287	8	exclusively	exclusively	RB
cord-299281-5z1xminb	287	9	from	from	IN
cord-299281-5z1xminb	287	10	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	287	11	digestion	digestion	NN
cord-299281-5z1xminb	287	12	of	of	IN
cord-299281-5z1xminb	287	13	SDS	sds	NN
cord-299281-5z1xminb	287	14	-	-	HYPH
cord-299281-5z1xminb	287	15	resistant	resistant	JJ
cord-299281-5z1xminb	287	16	Gml	Gml	NNP
cord-299281-5z1xminb	287	17	.	.	.
cord-299281-5z1xminb	288	1	This	this	DT
cord-299281-5z1xminb	288	2	would	would	MD
cord-299281-5z1xminb	288	3	suggest	suggest	VB
cord-299281-5z1xminb	288	4	that	that	IN
cord-299281-5z1xminb	288	5	the	the	DT
cord-299281-5z1xminb	288	6	last	last	JJ
cord-299281-5z1xminb	288	7	three	three	CD
cord-299281-5z1xminb	288	8	amino	amino	NN
cord-299281-5z1xminb	288	9	acids	acid	NNS
cord-299281-5z1xminb	288	10	of	of	IN
cord-299281-5z1xminb	288	11	the	the	DT
cord-299281-5z1xminb	288	12	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	288	13	tail	tail	NN
cord-299281-5z1xminb	288	14	of	of	IN
cord-299281-5z1xminb	288	15	Gml	Gml	NNP
cord-299281-5z1xminb	288	16	are	be	VBP
cord-299281-5z1xminb	288	17	required	require	VBN
cord-299281-5z1xminb	288	18	for	for	IN
cord-299281-5z1xminb	288	19	SDS	sds	NN
cord-299281-5z1xminb	288	20	resistance	resistance	NN
cord-299281-5z1xminb	288	21	of	of	IN
cord-299281-5z1xminb	288	22	the	the	DT
cord-299281-5z1xminb	288	23	oligomer	oligomer	NNP
cord-299281-5z1xminb	288	24	.	.	.
cord-299281-5z1xminb	289	1	Alternatively	alternatively	RB
cord-299281-5z1xminb	289	2	,	,	,
cord-299281-5z1xminb	289	3	trypsin	trypsin	NN
cord-299281-5z1xminb	289	4	may	may	MD
cord-299281-5z1xminb	289	5	destroy	destroy	VB
cord-299281-5z1xminb	289	6	another	another	DT
cord-299281-5z1xminb	289	7	(	(	-LRB-
cord-299281-5z1xminb	289	8	unlabeled	unlabeled	JJ
cord-299281-5z1xminb	289	9	)	)	-RRB-
cord-299281-5z1xminb	289	10	component	component	NN
cord-299281-5z1xminb	289	11	of	of	IN
cord-299281-5z1xminb	289	12	the	the	DT
cord-299281-5z1xminb	289	13	oligomer	oligomer	NN
cord-299281-5z1xminb	289	14	that	that	WDT
cord-299281-5z1xminb	289	15	is	be	VBZ
cord-299281-5z1xminb	289	16	required	require	VBN
cord-299281-5z1xminb	289	17	to	to	TO
cord-299281-5z1xminb	289	18	maintain	maintain	VB
cord-299281-5z1xminb	289	19	its	-PRON-	PRP$
cord-299281-5z1xminb	289	20	resistance	resistance	NN
cord-299281-5z1xminb	289	21	to	to	IN
cord-299281-5z1xminb	289	22	solubilization	solubilization	NN
cord-299281-5z1xminb	289	23	.	.	.
cord-299281-5z1xminb	290	1	Trypsinization	Trypsinization	NNP
cord-299281-5z1xminb	290	2	of	of	IN
cord-299281-5z1xminb	290	3	microsomes	microsome	NNS
cord-299281-5z1xminb	290	4	eliminated	eliminate	VBD
cord-299281-5z1xminb	290	5	the	the	DT
cord-299281-5z1xminb	290	6	SDS	sds	NN
cord-299281-5z1xminb	290	7	-	-	HYPH
cord-299281-5z1xminb	290	8	resistant	resistant	JJ
cord-299281-5z1xminb	290	9	form	form	NN
cord-299281-5z1xminb	290	10	of	of	IN
cord-299281-5z1xminb	290	11	Gml	Gml	NNP
cord-299281-5z1xminb	290	12	.	.	.
cord-299281-5z1xminb	291	1	To	to	TO
cord-299281-5z1xminb	291	2	ask	ask	VB
cord-299281-5z1xminb	291	3	if	if	IN
cord-299281-5z1xminb	291	4	proteolysis	proteolysis	NN
cord-299281-5z1xminb	291	5	dissociated	dissociate	VBD
cord-299281-5z1xminb	291	6	the	the	DT
cord-299281-5z1xminb	291	7	oligomers	oligomer	NNS
cord-299281-5z1xminb	291	8	as	as	RB
cord-299281-5z1xminb	291	9	well	well	RB
cord-299281-5z1xminb	291	10	,	,	,
cord-299281-5z1xminb	291	11	we	-PRON-	PRP
cord-299281-5z1xminb	291	12	subjected	subject	VBD
cord-299281-5z1xminb	291	13	trypsin	trypsin	NN
cord-299281-5z1xminb	291	14	-	-	HYPH
cord-299281-5z1xminb	291	15	treated	treat	VBN
cord-299281-5z1xminb	291	16	Gml	Gml	NNP
cord-299281-5z1xminb	291	17	-	-	HYPH
cord-299281-5z1xminb	291	18	containing	contain	VBG
cord-299281-5z1xminb	291	19	microsomes	microsome	NNS
cord-299281-5z1xminb	291	20	to	to	IN
cord-299281-5z1xminb	291	21	sucrose	sucrose	NN
cord-299281-5z1xminb	291	22	gradient	gradient	NN
cord-299281-5z1xminb	291	23	sedimentation	sedimentation	NN
cord-299281-5z1xminb	291	24	.	.	.
cord-299281-5z1xminb	292	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	292	2	prepared	prepare	VBD
cord-299281-5z1xminb	292	3	from	from	IN
cord-299281-5z1xminb	292	4	HeLa	HeLa	NNP
cord-299281-5z1xminb	292	5	cells	cell	NNS
cord-299281-5z1xminb	292	6	after	after	IN
cord-299281-5z1xminb	292	7	a	a	DT
cord-299281-5z1xminb	292	8	5-min	5-min	JJ
cord-299281-5z1xminb	292	9	pulse	pulse	NN
cord-299281-5z1xminb	292	10	and	and	CC
cord-299281-5z1xminb	292	11	a	a	DT
cord-299281-5z1xminb	292	12	60-min	60-min	CD
cord-299281-5z1xminb	292	13	chase	chase	NN
cord-299281-5z1xminb	292	14	were	be	VBD
cord-299281-5z1xminb	292	15	mock	mock	JJ
cord-299281-5z1xminb	292	16	-	-	HYPH
cord-299281-5z1xminb	292	17	treated	treat	VBN
cord-299281-5z1xminb	292	18	or	or	CC
cord-299281-5z1xminb	292	19	treated	treat	VBN
cord-299281-5z1xminb	292	20	with	with	IN
cord-299281-5z1xminb	292	21	TPCK	TPCK	NNP
cord-299281-5z1xminb	292	22	-	-	HYPH
cord-299281-5z1xminb	292	23	trypsin	trypsin	NN
cord-299281-5z1xminb	292	24	(	(	-LRB-
cord-299281-5z1xminb	292	25	20	20	CD
cord-299281-5z1xminb	292	26	#	#	$
cord-299281-5z1xminb	292	27	g	g	NN
cord-299281-5z1xminb	292	28	)	)	-RRB-
cord-299281-5z1xminb	292	29	for	for	IN
cord-299281-5z1xminb	292	30	30	30	CD
cord-299281-5z1xminb	292	31	min	min	NN
cord-299281-5z1xminb	292	32	at	at	IN
cord-299281-5z1xminb	292	33	37	37	CD
cord-299281-5z1xminb	292	34	°	°	NN
cord-299281-5z1xminb	292	35	C	c	NN
cord-299281-5z1xminb	292	36	.	.	.
cord-299281-5z1xminb	293	1	Samples	sample	NNS
cord-299281-5z1xminb	293	2	were	be	VBD
cord-299281-5z1xminb	293	3	diluted	dilute	VBN
cord-299281-5z1xminb	293	4	tenfold	tenfold	RB
cord-299281-5z1xminb	293	5	in	in	IN
cord-299281-5z1xminb	293	6	MNT	MNT	NNP
cord-299281-5z1xminb	293	7	,	,	,
cord-299281-5z1xminb	293	8	and	and	CC
cord-299281-5z1xminb	293	9	aliquots	aliquot	NNS
cord-299281-5z1xminb	293	10	were	be	VBD
cord-299281-5z1xminb	293	11	loaded	load	VBN
cord-299281-5z1xminb	293	12	on	on	IN
cord-299281-5z1xminb	293	13	sucrose	sucrose	JJ
cord-299281-5z1xminb	293	14	gradients	gradient	NNS
cord-299281-5z1xminb	293	15	and	and	CC
cord-299281-5z1xminb	293	16	centrifuged	centrifuge	VBD
cord-299281-5z1xminb	293	17	as	as	IN
cord-299281-5z1xminb	293	18	described	describe	VBN
cord-299281-5z1xminb	293	19	in	in	IN
cord-299281-5z1xminb	293	20	Materials	material	NNS
cord-299281-5z1xminb	293	21	and	and	CC
cord-299281-5z1xminb	293	22	Methods	method	NNS
cord-299281-5z1xminb	293	23	.	.	.
cord-299281-5z1xminb	294	1	Gradient	gradient	NN
cord-299281-5z1xminb	294	2	fractions	fraction	NNS
cord-299281-5z1xminb	294	3	were	be	VBD
cord-299281-5z1xminb	294	4	collected	collect	VBN
cord-299281-5z1xminb	294	5	,	,	,
cord-299281-5z1xminb	294	6	immunoprecipitated	immunoprecipitated	JJ
cord-299281-5z1xminb	294	7	,	,	,
cord-299281-5z1xminb	294	8	and	and	CC
cord-299281-5z1xminb	294	9	analyzed	analyze	VBN
cord-299281-5z1xminb	294	10	by	by	IN
cord-299281-5z1xminb	294	11	SDS	SDS	NNP
cord-299281-5z1xminb	294	12	-	-	HYPH
cord-299281-5z1xminb	294	13	PAGE	PAGE	NNP
cord-299281-5z1xminb	294	14	.	.	.
cord-299281-5z1xminb	295	1	The	the	DT
cord-299281-5z1xminb	295	2	trypsin	trypsin	NN
cord-299281-5z1xminb	295	3	-	-	HYPH
cord-299281-5z1xminb	295	4	treated	treat	VBN
cord-299281-5z1xminb	295	5	samples	sample	NNS
cord-299281-5z1xminb	295	6	are	be	VBP
cord-299281-5z1xminb	295	7	shown	show	VBN
cord-299281-5z1xminb	295	8	in	in	IN
cord-299281-5z1xminb	295	9	Fig	Fig	NNP
cord-299281-5z1xminb	295	10	.	.	.
cord-299281-5z1xminb	296	1	9	9	LS
cord-299281-5z1xminb	296	2	.	.	.
cord-299281-5z1xminb	297	1	Trypsin	Trypsin	NNP
cord-299281-5z1xminb	297	2	digestion	digestion	NN
cord-299281-5z1xminb	297	3	did	do	VBD
cord-299281-5z1xminb	297	4	not	not	RB
cord-299281-5z1xminb	297	5	cause	cause	VB
cord-299281-5z1xminb	297	6	dissociation	dissociation	NN
cord-299281-5z1xminb	297	7	of	of	IN
cord-299281-5z1xminb	297	8	VSV	VSV	NNP
cord-299281-5z1xminb	297	9	G	G	NNP
cord-299281-5z1xminb	297	10	trimers	trimer	NNS
cord-299281-5z1xminb	297	11	(	(	-LRB-
cord-299281-5z1xminb	297	12	compare	compare	VB
cord-299281-5z1xminb	297	13	Fig	Fig	NNP
cord-299281-5z1xminb	297	14	.	.	.
cord-299281-5z1xminb	298	1	9	9	LS
cord-299281-5z1xminb	298	2	A	a	NN
cord-299281-5z1xminb	298	3	with	with	IN
cord-299281-5z1xminb	298	4	Fig	Fig	NNP
cord-299281-5z1xminb	298	5	.	.	NNP
cord-299281-5z1xminb	298	6	5	5	CD
cord-299281-5z1xminb	298	7	)	)	-RRB-
cord-299281-5z1xminb	298	8	.	.	.
cord-299281-5z1xminb	299	1	Significantly	significantly	RB
cord-299281-5z1xminb	299	2	,	,	,
cord-299281-5z1xminb	299	3	the	the	DT
cord-299281-5z1xminb	299	4	larger	large	JJR
cord-299281-5z1xminb	299	5	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	299	6	product	product	NN
cord-299281-5z1xminb	299	7	of	of	IN
cord-299281-5z1xminb	299	8	Gml	Gml	NNP
cord-299281-5z1xminb	299	9	migrated	migrate	VBD
cord-299281-5z1xminb	299	10	at	at	IN
cord-299281-5z1xminb	299	11	the	the	DT
cord-299281-5z1xminb	299	12	bottom	bottom	NN
cord-299281-5z1xminb	299	13	of	of	IN
cord-299281-5z1xminb	299	14	the	the	DT
cord-299281-5z1xminb	299	15	gradient	gradient	NN
cord-299281-5z1xminb	299	16	,	,	,
cord-299281-5z1xminb	299	17	whereas	whereas	IN
cord-299281-5z1xminb	299	18	some	some	DT
cord-299281-5z1xminb	299	19	of	of	IN
cord-299281-5z1xminb	299	20	the	the	DT
cord-299281-5z1xminb	299	21	smaller	small	JJR
cord-299281-5z1xminb	299	22	product	product	NN
cord-299281-5z1xminb	299	23	pelleted	pellete	VBN
cord-299281-5z1xminb	299	24	and	and	CC
cord-299281-5z1xminb	299	25	some	some	DT
cord-299281-5z1xminb	299	26	was	be	VBD
cord-299281-5z1xminb	299	27	found	find	VBN
cord-299281-5z1xminb	299	28	throughout	throughout	IN
cord-299281-5z1xminb	299	29	the	the	DT
cord-299281-5z1xminb	299	30	gradient	gradient	NN
cord-299281-5z1xminb	299	31	(	(	-LRB-
cord-299281-5z1xminb	299	32	Fig	Fig	NNP
cord-299281-5z1xminb	299	33	.	.	.
cord-299281-5z1xminb	299	34	9	9	CD
cord-299281-5z1xminb	299	35	B	b	NN
cord-299281-5z1xminb	299	36	)	)	-RRB-
cord-299281-5z1xminb	299	37	.	.	.
cord-299281-5z1xminb	300	1	This	this	DT
cord-299281-5z1xminb	300	2	suggested	suggest	VBD
cord-299281-5z1xminb	300	3	that	that	IN
cord-299281-5z1xminb	300	4	removing	remove	VBG
cord-299281-5z1xminb	300	5	part	part	NN
cord-299281-5z1xminb	300	6	of	of	IN
cord-299281-5z1xminb	300	7	the	the	DT
cord-299281-5z1xminb	300	8	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	300	9	tail	tail	NN
cord-299281-5z1xminb	300	10	of	of	IN
cord-299281-5z1xminb	300	11	Gml	Gml	NNP
cord-299281-5z1xminb	300	12	does	do	VBZ
cord-299281-5z1xminb	300	13	not	not	RB
cord-299281-5z1xminb	300	14	disrupt	disrupt	VB
cord-299281-5z1xminb	300	15	the	the	DT
cord-299281-5z1xminb	300	16	oligomer	oligomer	NN
cord-299281-5z1xminb	300	17	,	,	,
cord-299281-5z1xminb	300	18	and	and	CC
cord-299281-5z1xminb	300	19	further	further	RB
cord-299281-5z1xminb	300	20	supports	support	VBZ
cord-299281-5z1xminb	300	21	the	the	DT
cord-299281-5z1xminb	300	22	possibility	possibility	NN
cord-299281-5z1xminb	300	23	that	that	IN
cord-299281-5z1xminb	300	24	digestion	digestion	NN
cord-299281-5z1xminb	300	25	of	of	IN
cord-299281-5z1xminb	300	26	SDS	sds	NN
cord-299281-5z1xminb	300	27	-	-	HYPH
cord-299281-5z1xminb	300	28	resistant	resistant	JJ
cord-299281-5z1xminb	300	29	material	material	NN
cord-299281-5z1xminb	300	30	gives	give	VBZ
cord-299281-5z1xminb	300	31	rise	rise	NN
cord-299281-5z1xminb	300	32	to	to	IN
cord-299281-5z1xminb	300	33	the	the	DT
cord-299281-5z1xminb	300	34	larger	large	JJR
cord-299281-5z1xminb	300	35	band	band	NN
cord-299281-5z1xminb	300	36	.	.	.
cord-299281-5z1xminb	301	1	We	-PRON-	PRP
cord-299281-5z1xminb	301	2	tested	test	VBD
cord-299281-5z1xminb	301	3	the	the	DT
cord-299281-5z1xminb	301	4	possibility	possibility	NN
cord-299281-5z1xminb	301	5	of	of	IN
cord-299281-5z1xminb	301	6	cytoskeletal	cytoskeletal	JJ
cord-299281-5z1xminb	301	7	involvement	involvement	NN
cord-299281-5z1xminb	301	8	in	in	IN
cord-299281-5z1xminb	301	9	SDS	SDS	NNP
cord-299281-5z1xminb	301	10	resistance	resistance	NN
cord-299281-5z1xminb	301	11	of	of	IN
cord-299281-5z1xminb	301	12	Gml	Gml	NNP
cord-299281-5z1xminb	301	13	by	by	IN
cord-299281-5z1xminb	301	14	pretreating	pretreate	VBG
cord-299281-5z1xminb	301	15	cells	cell	NNS
cord-299281-5z1xminb	301	16	with	with	IN
cord-299281-5z1xminb	301	17	drugs	drug	NNS
cord-299281-5z1xminb	301	18	that	that	WDT
cord-299281-5z1xminb	301	19	disrupt	disrupt	VBP
cord-299281-5z1xminb	301	20	microtubules	microtubule	NNS
cord-299281-5z1xminb	301	21	or	or	CC
cord-299281-5z1xminb	301	22	actin	actin	NN
cord-299281-5z1xminb	301	23	filaments	filament	NNS
cord-299281-5z1xminb	301	24	.	.	.
cord-299281-5z1xminb	302	1	Depolymerization	Depolymerization	NNP
cord-299281-5z1xminb	302	2	of	of	IN
cord-299281-5z1xminb	302	3	microtubules	microtubule	NNS
cord-299281-5z1xminb	302	4	with	with	IN
cord-299281-5z1xminb	302	5	colchicine	colchicine	NNP
cord-299281-5z1xminb	302	6	(	(	-LRB-
cord-299281-5z1xminb	302	7	50/~M	50/~m	CD
cord-299281-5z1xminb	302	8	)	)	-RRB-
cord-299281-5z1xminb	302	9	had	have	VBD
cord-299281-5z1xminb	302	10	no	no	DT
cord-299281-5z1xminb	302	11	effect	effect	NN
cord-299281-5z1xminb	302	12	on	on	IN
cord-299281-5z1xminb	302	13	the	the	DT
cord-299281-5z1xminb	302	14	amount	amount	NN
cord-299281-5z1xminb	302	15	of	of	IN
cord-299281-5z1xminb	302	16	SDS	sds	NN
cord-299281-5z1xminb	302	17	-	-	HYPH
cord-299281-5z1xminb	302	18	resistant	resistant	JJ
cord-299281-5z1xminb	302	19	Gml	Gml	NNP
cord-299281-5z1xminb	302	20	formed	form	VBN
cord-299281-5z1xminb	302	21	(	(	-LRB-
cord-299281-5z1xminb	302	22	not	not	RB
cord-299281-5z1xminb	302	23	shown	show	VBN
cord-299281-5z1xminb	302	24	)	)	-RRB-
cord-299281-5z1xminb	302	25	.	.	.
cord-299281-5z1xminb	303	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	303	2	,	,	,
cord-299281-5z1xminb	303	3	treatment	treatment	NN
cord-299281-5z1xminb	303	4	with	with	IN
cord-299281-5z1xminb	303	5	cytochalasin	cytochalasin	FW
cord-299281-5z1xminb	303	6	D	D	NNP
cord-299281-5z1xminb	303	7	(	(	-LRB-
cord-299281-5z1xminb	303	8	100	100	CD
cord-299281-5z1xminb	303	9	/	/	SYM
cord-299281-5z1xminb	303	10	zM	zm	NN
cord-299281-5z1xminb	303	11	)	)	-RRB-
cord-299281-5z1xminb	303	12	starting	start	VBG
cord-299281-5z1xminb	303	13	1	1	CD
cord-299281-5z1xminb	303	14	h	h	NN
cord-299281-5z1xminb	303	15	before	before	IN
cord-299281-5z1xminb	303	16	metabolic	metabolic	JJ
cord-299281-5z1xminb	303	17	labeling	labeling	NN
cord-299281-5z1xminb	303	18	blocked	block	VBN
cord-299281-5z1xminb	303	19	formation	formation	NN
cord-299281-5z1xminb	303	20	of	of	IN
cord-299281-5z1xminb	303	21	SDS	sds	NN
cord-299281-5z1xminb	303	22	-	-	HYPH
cord-299281-5z1xminb	303	23	resistant	resistant	JJ
cord-299281-5z1xminb	303	24	Gml	Gml	NNP
cord-299281-5z1xminb	303	25	oligomers	oligomer	NNS
cord-299281-5z1xminb	303	26	(	(	-LRB-
cord-299281-5z1xminb	303	27	Fig	Fig	NNP
cord-299281-5z1xminb	303	28	.	.	NNP
cord-299281-5z1xminb	303	29	10	10	CD
cord-299281-5z1xminb	303	30	,	,	,
cord-299281-5z1xminb	303	31	lane	lane	NN
cord-299281-5z1xminb	303	32	3	3	CD
cord-299281-5z1xminb	303	33	)	)	-RRB-
cord-299281-5z1xminb	303	34	.	.	.
cord-299281-5z1xminb	304	1	The	the	DT
cord-299281-5z1xminb	304	2	amount	amount	NN
cord-299281-5z1xminb	304	3	of	of	IN
cord-299281-5z1xminb	304	4	immunoprecipitated	immunoprecipitated	JJ
cord-299281-5z1xminb	304	5	radiolabel	radiolabel	NNP
cord-299281-5z1xminb	304	6	migrating	migrate	VBG
cord-299281-5z1xminb	304	7	at	at	IN
cord-299281-5z1xminb	304	8	the	the	DT
cord-299281-5z1xminb	304	9	top	top	NN
cord-299281-5z1xminb	304	10	of	of	IN
cord-299281-5z1xminb	304	11	the	the	DT
cord-299281-5z1xminb	304	12	stacking	stacking	NN
cord-299281-5z1xminb	304	13	gel	gel	NN
cord-299281-5z1xminb	304	14	decreased	decrease	VBD
cord-299281-5z1xminb	304	15	from	from	IN
cord-299281-5z1xminb	304	16	43	43	CD
cord-299281-5z1xminb	304	17	%	%	NN
cord-299281-5z1xminb	304	18	of	of	IN
cord-299281-5z1xminb	304	19	total	total	NN
cord-299281-5z1xminb	304	20	in	in	IN
cord-299281-5z1xminb	304	21	untreated	untreated	JJ
cord-299281-5z1xminb	304	22	cells	cell	NNS
cord-299281-5z1xminb	304	23	to	to	IN
cord-299281-5z1xminb	304	24	18	18	CD
cord-299281-5z1xminb	304	25	%	%	NN
cord-299281-5z1xminb	304	26	of	of	IN
cord-299281-5z1xminb	304	27	total	total	NN
cord-299281-5z1xminb	304	28	in	in	IN
cord-299281-5z1xminb	304	29	cytochalasin	cytochalasin	FW
cord-299281-5z1xminb	304	30	D	d	NN
cord-299281-5z1xminb	304	31	-	-	HYPH
cord-299281-5z1xminb	304	32	treated	treat	VBN
cord-299281-5z1xminb	304	33	cells	cell	NNS
cord-299281-5z1xminb	304	34	(	(	-LRB-
cord-299281-5z1xminb	304	35	compare	compare	VBP
cord-299281-5z1xminb	304	36	Fig	Fig	NNP
cord-299281-5z1xminb	304	37	.	.	NNP
cord-299281-5z1xminb	304	38	10	10	CD
cord-299281-5z1xminb	304	39	,	,	,
cord-299281-5z1xminb	304	40	lanes	lane	NNS
cord-299281-5z1xminb	304	41	I	-PRON-	PRP
cord-299281-5z1xminb	304	42	and	and	CC
cord-299281-5z1xminb	304	43	3	3	CD
cord-299281-5z1xminb	304	44	)	)	-RRB-
cord-299281-5z1xminb	304	45	.	.	.
cord-299281-5z1xminb	305	1	Cytochalasin	Cytochalasin	NNP
cord-299281-5z1xminb	305	2	D	D	NNP
cord-299281-5z1xminb	305	3	did	do	VBD
cord-299281-5z1xminb	305	4	not	not	RB
cord-299281-5z1xminb	305	5	block	block	VB
cord-299281-5z1xminb	305	6	the	the	DT
cord-299281-5z1xminb	305	7	transport	transport	NN
cord-299281-5z1xminb	305	8	of	of	IN
cord-299281-5z1xminb	305	9	VSV	VSV	NNP
cord-299281-5z1xminb	305	10	G	G	NNP
cord-299281-5z1xminb	305	11	since	since	IN
cord-299281-5z1xminb	305	12	the	the	DT
cord-299281-5z1xminb	305	13	rate	rate	NN
cord-299281-5z1xminb	305	14	of	of	IN
cord-299281-5z1xminb	305	15	sialylation	sialylation	NN
cord-299281-5z1xminb	305	16	was	be	VBD
cord-299281-5z1xminb	305	17	enhanced	enhance	VBN
cord-299281-5z1xminb	305	18	relative	relative	NN
cord-299281-5z1xminb	305	19	to	to	IN
cord-299281-5z1xminb	305	20	untreated	untreated	JJ
cord-299281-5z1xminb	305	21	cells	cell	NNS
cord-299281-5z1xminb	305	22	.	.	.
cord-299281-5z1xminb	306	1	On	on	IN
cord-299281-5z1xminb	306	2	sucrose	sucrose	JJ
cord-299281-5z1xminb	306	3	gradients	gradient	NNS
cord-299281-5z1xminb	306	4	,	,	,
cord-299281-5z1xminb	306	5	Gml	Gml	NNP
cord-299281-5z1xminb	306	6	solubilized	solubilize	VBN
cord-299281-5z1xminb	306	7	from	from	IN
cord-299281-5z1xminb	306	8	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	306	9	D	D	NNP
cord-299281-5z1xminb	306	10	-	-	HYPH
cord-299281-5z1xminb	306	11	treated	treat	VBN
cord-299281-5z1xminb	306	12	cells	cell	NNS
cord-299281-5z1xminb	306	13	migrated	migrate	VBD
cord-299281-5z1xminb	306	14	as	as	IN
cord-299281-5z1xminb	306	15	an	an	DT
cord-299281-5z1xminb	306	16	SDS	SDS	NNP
cord-299281-5z1xminb	306	17	-	-	HYPH
cord-299281-5z1xminb	306	18	sensitive	sensitive	JJ
cord-299281-5z1xminb	306	19	oligomer	oligomer	NNP
cord-299281-5z1xminb	306	20	(	(	-LRB-
cord-299281-5z1xminb	306	21	in	in	IN
cord-299281-5z1xminb	306	22	the	the	DT
cord-299281-5z1xminb	306	23	pellet	pellet	NN
cord-299281-5z1xminb	306	24	)	)	-RRB-
cord-299281-5z1xminb	306	25	,	,	,
cord-299281-5z1xminb	306	26	and	and	CC
cord-299281-5z1xminb	306	27	VSV	VSV	NNP
cord-299281-5z1xminb	306	28	G	G	NNP
cord-299281-5z1xminb	306	29	trimerization	trimerization	NN
cord-299281-5z1xminb	306	30	was	be	VBD
cord-299281-5z1xminb	306	31	unaffected	unaffected	JJ
cord-299281-5z1xminb	306	32	(	(	-LRB-
cord-299281-5z1xminb	306	33	data	datum	NNS
cord-299281-5z1xminb	306	34	not	not	RB
cord-299281-5z1xminb	306	35	shown	show	VBN
cord-299281-5z1xminb	306	36	)	)	-RRB-
cord-299281-5z1xminb	306	37	.	.	.
cord-299281-5z1xminb	307	1	This	this	DT
cord-299281-5z1xminb	307	2	observation	observation	NN
cord-299281-5z1xminb	307	3	suggests	suggest	VBZ
cord-299281-5z1xminb	307	4	that	that	IN
cord-299281-5z1xminb	307	5	oligomer	oligomer	NNP
cord-299281-5z1xminb	307	6	formation	formation	NN
cord-299281-5z1xminb	307	7	and	and	CC
cord-299281-5z1xminb	307	8	SDS	SDS	NNP
cord-299281-5z1xminb	307	9	-	-	HYPH
cord-299281-5z1xminb	307	10	resistance	resistance	NN
cord-299281-5z1xminb	307	11	are	be	VBP
cord-299281-5z1xminb	307	12	separable	separable	JJ
cord-299281-5z1xminb	307	13	characteristics	characteristic	NNS
cord-299281-5z1xminb	307	14	,	,	,
cord-299281-5z1xminb	307	15	and	and	CC
cord-299281-5z1xminb	307	16	furthermore	furthermore	RB
cord-299281-5z1xminb	307	17	implies	imply	VBZ
cord-299281-5z1xminb	307	18	that	that	IN
cord-299281-5z1xminb	307	19	SDS	sds	NN
cord-299281-5z1xminb	307	20	-	-	HYPH
cord-299281-5z1xminb	307	21	resistance	resistance	NN
cord-299281-5z1xminb	307	22	may	may	MD
cord-299281-5z1xminb	307	23	result	result	VB
cord-299281-5z1xminb	307	24	from	from	IN
cord-299281-5z1xminb	307	25	interaction	interaction	NN
cord-299281-5z1xminb	307	26	between	between	IN
cord-299281-5z1xminb	307	27	Gml	Gml	NNP
cord-299281-5z1xminb	307	28	and	and	CC
cord-299281-5z1xminb	307	29	an	an	DT
cord-299281-5z1xminb	307	30	actin	actin	NN
cord-299281-5z1xminb	307	31	-	-	HYPH
cord-299281-5z1xminb	307	32	based	base	VBN
cord-299281-5z1xminb	307	33	matrix	matrix	NN
cord-299281-5z1xminb	307	34	or	or	CC
cord-299281-5z1xminb	307	35	cytoskeleton	cytoskeleton	NN
cord-299281-5z1xminb	307	36	.	.	.
cord-299281-5z1xminb	308	1	Because	because	IN
cord-299281-5z1xminb	308	2	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	308	3	D	D	NNP
cord-299281-5z1xminb	308	4	blocked	block	VBD
cord-299281-5z1xminb	308	5	the	the	DT
cord-299281-5z1xminb	308	6	formation	formation	NN
cord-299281-5z1xminb	308	7	of	of	IN
cord-299281-5z1xminb	308	8	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	308	9	oligomers	oligomer	NNS
cord-299281-5z1xminb	308	10	of	of	IN
cord-299281-5z1xminb	308	11	Gml	Gml	NNP
cord-299281-5z1xminb	308	12	,	,	,
cord-299281-5z1xminb	308	13	we	-PRON-	PRP
cord-299281-5z1xminb	308	14	tested	test	VBD
cord-299281-5z1xminb	308	15	whether	whether	IN
cord-299281-5z1xminb	308	16	this	this	DT
cord-299281-5z1xminb	308	17	treatment	treatment	NN
cord-299281-5z1xminb	308	18	prevented	prevent	VBD
cord-299281-5z1xminb	308	19	the	the	DT
cord-299281-5z1xminb	308	20	appearance	appearance	NN
cord-299281-5z1xminb	308	21	of	of	IN
cord-299281-5z1xminb	308	22	the	the	DT
cord-299281-5z1xminb	308	23	larger	large	JJR
cord-299281-5z1xminb	308	24	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	308	25	band	band	NN
cord-299281-5z1xminb	308	26	.	.	.
cord-299281-5z1xminb	309	1	HeLa	HeLa	NNP
cord-299281-5z1xminb	309	2	cells	cell	NNS
cord-299281-5z1xminb	309	3	expressing	express	VBG
cord-299281-5z1xminb	309	4	Gml	Gml	NNP
cord-299281-5z1xminb	309	5	or	or	CC
cord-299281-5z1xminb	309	6	VSV	VSV	NNP
cord-299281-5z1xminb	309	7	G	G	NNP
cord-299281-5z1xminb	309	8	were	be	VBD
cord-299281-5z1xminb	309	9	treated	treat	VBN
cord-299281-5z1xminb	309	10	with	with	IN
cord-299281-5z1xminb	309	11	cytochalasin	cytochalasin	FW
cord-299281-5z1xminb	309	12	D	D	NNP
cord-299281-5z1xminb	309	13	(	(	-LRB-
cord-299281-5z1xminb	309	14	100	100	CD
cord-299281-5z1xminb	309	15	/	/	SYM
cord-299281-5z1xminb	309	16	zM	zm	NN
cord-299281-5z1xminb	309	17	)	)	-RRB-
cord-299281-5z1xminb	309	18	for	for	IN
cord-299281-5z1xminb	309	19	1	1	CD
cord-299281-5z1xminb	309	20	h	h	NN
cord-299281-5z1xminb	309	21	before	before	IN
cord-299281-5z1xminb	309	22	a	a	DT
cord-299281-5z1xminb	309	23	15-min	15-min	CD
cord-299281-5z1xminb	309	24	metabolic	metabolic	JJ
cord-299281-5z1xminb	309	25	labeling	labeling	NN
cord-299281-5z1xminb	309	26	.	.	.
cord-299281-5z1xminb	310	1	Microsomes	microsome	NNS
cord-299281-5z1xminb	310	2	were	be	VBD
cord-299281-5z1xminb	310	3	prepared	prepare	VBN
cord-299281-5z1xminb	310	4	after	after	IN
cord-299281-5z1xminb	310	5	a	a	DT
cord-299281-5z1xminb	310	6	45-min	45-min	CD
cord-299281-5z1xminb	310	7	chase	chase	NN
cord-299281-5z1xminb	310	8	and	and	CC
cord-299281-5z1xminb	310	9	mock	mock	NN
cord-299281-5z1xminb	310	10	-	-	HYPH
cord-299281-5z1xminb	310	11	treated	treat	VBN
cord-299281-5z1xminb	310	12	or	or	CC
cord-299281-5z1xminb	310	13	treated	treat	VBN
cord-299281-5z1xminb	310	14	with	with	IN
cord-299281-5z1xminb	310	15	20	20	CD
cord-299281-5z1xminb	310	16	/	/	SYM
cord-299281-5z1xminb	310	17	zg	zg	NN
cord-299281-5z1xminb	310	18	of	of	IN
cord-299281-5z1xminb	310	19	TPCK	TPCK	NNP
cord-299281-5z1xminb	310	20	-	-	HYPH
cord-299281-5z1xminb	310	21	trypsin	trypsin	NN
cord-299281-5z1xminb	310	22	for	for	IN
cord-299281-5z1xminb	310	23	30	30	CD
cord-299281-5z1xminb	310	24	min	min	NN
cord-299281-5z1xminb	310	25	at	at	IN
cord-299281-5z1xminb	310	26	37	37	CD
cord-299281-5z1xminb	310	27	°	°	NN
cord-299281-5z1xminb	310	28	C	c	NN
cord-299281-5z1xminb	310	29	,	,	,
cord-299281-5z1xminb	310	30	and	and	CC
cord-299281-5z1xminb	310	31	then	then	RB
cord-299281-5z1xminb	310	32	solubilized	solubilize	VBD
cord-299281-5z1xminb	310	33	and	and	CC
cord-299281-5z1xminb	310	34	immunoprecipitated	immunoprecipitate	VBD
cord-299281-5z1xminb	310	35	using	use	VBG
cord-299281-5z1xminb	310	36	anti	anti	JJ
cord-299281-5z1xminb	310	37	-	-	JJ
cord-299281-5z1xminb	310	38	VSV	VSV	NNP
cord-299281-5z1xminb	310	39	antibody	antibody	NN
cord-299281-5z1xminb	311	1	(	(	-LRB-
cord-299281-5z1xminb	311	2	Fig	Fig	NNP
cord-299281-5z1xminb	311	3	.	.	.
cord-299281-5z1xminb	311	4	10	10	CD
cord-299281-5z1xminb	311	5	)	)	-RRB-
cord-299281-5z1xminb	311	6	.	.	.
cord-299281-5z1xminb	312	1	As	as	IN
cord-299281-5z1xminb	312	2	mentioned	mention	VBN
cord-299281-5z1xminb	312	3	above	above	RB
cord-299281-5z1xminb	312	4	,	,	,
cord-299281-5z1xminb	312	5	treatment	treatment	NN
cord-299281-5z1xminb	312	6	with	with	IN
cord-299281-5z1xminb	312	7	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	312	8	D	D	NNP
cord-299281-5z1xminb	312	9	enhanced	enhance	VBD
cord-299281-5z1xminb	312	10	the	the	DT
cord-299281-5z1xminb	312	11	fraction	fraction	NN
cord-299281-5z1xminb	312	12	ofVSV	ofvsv	CD
cord-299281-5z1xminb	312	13	G	g	NN
cord-299281-5z1xminb	312	14	that	that	WDT
cord-299281-5z1xminb	312	15	became	become	VBD
cord-299281-5z1xminb	312	16	sialylated	sialylated	JJ
cord-299281-5z1xminb	312	17	during	during	IN
cord-299281-5z1xminb	312	18	this	this	DT
cord-299281-5z1xminb	312	19	period	period	NN
cord-299281-5z1xminb	312	20	(	(	-LRB-
cord-299281-5z1xminb	312	21	compare	compare	VB
cord-299281-5z1xminb	312	22	lanes	lane	NNS
cord-299281-5z1xminb	312	23	5	5	CD
cord-299281-5z1xminb	312	24	and	and	CC
cord-299281-5z1xminb	312	25	7	7	CD
cord-299281-5z1xminb	312	26	)	)	-RRB-
cord-299281-5z1xminb	312	27	.	.	.
cord-299281-5z1xminb	313	1	We	-PRON-	PRP
cord-299281-5z1xminb	313	2	routinely	routinely	RB
cord-299281-5z1xminb	313	3	observed	observe	VBD
cord-299281-5z1xminb	313	4	that	that	IN
cord-299281-5z1xminb	313	5	trypsin	trypsin	NN
cord-299281-5z1xminb	313	6	degraded	degrade	VBD
cord-299281-5z1xminb	313	7	most	most	JJS
cord-299281-5z1xminb	313	8	of	of	IN
cord-299281-5z1xminb	313	9	the	the	DT
cord-299281-5z1xminb	313	10	VSV	VSV	NNP
cord-299281-5z1xminb	313	11	G	G	NNP
cord-299281-5z1xminb	313	12	recovered	recover	VBD
cord-299281-5z1xminb	313	13	from	from	IN
cord-299281-5z1xminb	313	14	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	313	15	D	D	NNP
cord-299281-5z1xminb	313	16	-	-	HYPH
cord-299281-5z1xminb	313	17	treated	treat	VBN
cord-299281-5z1xminb	313	18	cells	cell	NNS
cord-299281-5z1xminb	313	19	(	(	-LRB-
cord-299281-5z1xminb	313	20	lane	lane	NNP
cord-299281-5z1xminb	313	21	8)	8)	NNP
cord-299281-5z1xminb	313	22	,	,	,
cord-299281-5z1xminb	313	23	suggesting	suggest	VBG
cord-299281-5z1xminb	313	24	that	that	IN
cord-299281-5z1xminb	313	25	the	the	DT
cord-299281-5z1xminb	313	26	orientation	orientation	NN
cord-299281-5z1xminb	313	27	or	or	CC
cord-299281-5z1xminb	313	28	durability	durability	NN
cord-299281-5z1xminb	313	29	of	of	IN
cord-299281-5z1xminb	313	30	microsomes	microsome	NNS
cord-299281-5z1xminb	313	31	was	be	VBD
cord-299281-5z1xminb	313	32	affected	affect	VBN
cord-299281-5z1xminb	313	33	by	by	IN
cord-299281-5z1xminb	313	34	treatment	treatment	NN
cord-299281-5z1xminb	313	35	with	with	IN
cord-299281-5z1xminb	313	36	this	this	DT
cord-299281-5z1xminb	313	37	perturbant	perturbant	NN
cord-299281-5z1xminb	313	38	.	.	.
cord-299281-5z1xminb	314	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	314	2	,	,	,
cord-299281-5z1xminb	314	3	trypsin	trypsin	JJ
cord-299281-5z1xminb	314	4	digestion	digestion	NN
cord-299281-5z1xminb	314	5	of	of	IN
cord-299281-5z1xminb	314	6	Gml	Gml	NNP
cord-299281-5z1xminb	314	7	recovered	recover	VBN
cord-299281-5z1xminb	314	8	from	from	IN
cord-299281-5z1xminb	314	9	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	314	10	D	D	NNP
cord-299281-5z1xminb	314	11	-	-	HYPH
cord-299281-5z1xminb	314	12	treated	treat	VBN
cord-299281-5z1xminb	314	13	cells	cell	NNS
cord-299281-5z1xminb	314	14	yielded	yield	VBD
cord-299281-5z1xminb	314	15	predominantly	predominantly	RB
cord-299281-5z1xminb	314	16	the	the	DT
cord-299281-5z1xminb	314	17	smaller	small	JJR
cord-299281-5z1xminb	314	18	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	314	19	species	specie	NNS
cord-299281-5z1xminb	314	20	,	,	,
cord-299281-5z1xminb	314	21	generated	generate	VBN
cord-299281-5z1xminb	314	22	by	by	IN
cord-299281-5z1xminb	314	23	cleavage	cleavage	NN
cord-299281-5z1xminb	314	24	within	within	IN
cord-299281-5z1xminb	314	25	region	region	NN
cord-299281-5z1xminb	314	26	B	b	NN
cord-299281-5z1xminb	314	27	in	in	IN
cord-299281-5z1xminb	314	28	Fig	Fig	NNP
cord-299281-5z1xminb	314	29	.	.	.
cord-299281-5z1xminb	315	1	8	8	CD
cord-299281-5z1xminb	315	2	A.	a.	NN
cord-299281-5z1xminb	316	1	We	-PRON-	PRP
cord-299281-5z1xminb	316	2	therefore	therefore	RB
cord-299281-5z1xminb	316	3	conclude	conclude	VBP
cord-299281-5z1xminb	316	4	that	that	IN
cord-299281-5z1xminb	316	5	the	the	DT
cord-299281-5z1xminb	316	6	larger	large	JJR
cord-299281-5z1xminb	316	7	Gml	Gml	NNP
cord-299281-5z1xminb	316	8	species	species	NN
cord-299281-5z1xminb	316	9	does	do	VBZ
cord-299281-5z1xminb	316	10	indeed	indeed	RB
cord-299281-5z1xminb	316	11	represent	represent	VB
cord-299281-5z1xminb	316	12	material	material	NN
cord-299281-5z1xminb	316	13	digested	digest	VBN
cord-299281-5z1xminb	316	14	from	from	IN
cord-299281-5z1xminb	316	15	SDSresistant	sdsresistant	JJ
cord-299281-5z1xminb	316	16	oligomers	oligomer	NNS
cord-299281-5z1xminb	316	17	,	,	,
cord-299281-5z1xminb	316	18	and	and	CC
cord-299281-5z1xminb	316	19	furthermore	furthermore	RB
cord-299281-5z1xminb	316	20	,	,	,
cord-299281-5z1xminb	316	21	that	that	IN
cord-299281-5z1xminb	316	22	the	the	DT
cord-299281-5z1xminb	316	23	oligomers	oligomer	NNS
cord-299281-5z1xminb	316	24	may	may	MD
cord-299281-5z1xminb	316	25	interact	interact	VB
cord-299281-5z1xminb	316	26	with	with	IN
cord-299281-5z1xminb	316	27	an	an	DT
cord-299281-5z1xminb	316	28	actin	actin	NN
cord-299281-5z1xminb	316	29	-	-	HYPH
cord-299281-5z1xminb	316	30	based	base	VBN
cord-299281-5z1xminb	316	31	cytoskeleton	cytoskeleton	NN
cord-299281-5z1xminb	316	32	.	.	.
cord-299281-5z1xminb	317	1	The	the	DT
cord-299281-5z1xminb	317	2	experiments	experiment	NNS
cord-299281-5z1xminb	317	3	presented	present	VBD
cord-299281-5z1xminb	317	4	here	here	RB
cord-299281-5z1xminb	317	5	support	support	VBP
cord-299281-5z1xminb	317	6	protein	protein	NN
cord-299281-5z1xminb	317	7	oligomerization	oligomerization	NN
cord-299281-5z1xminb	317	8	as	as	IN
cord-299281-5z1xminb	317	9	a	a	DT
cord-299281-5z1xminb	317	10	mechanism	mechanism	NN
cord-299281-5z1xminb	317	11	for	for	IN
cord-299281-5z1xminb	317	12	retaining	retain	VBG
cord-299281-5z1xminb	317	13	the	the	DT
cord-299281-5z1xminb	317	14	chimeric	chimeric	JJ
cord-299281-5z1xminb	317	15	protein	protein	NN
cord-299281-5z1xminb	317	16	Gml	Gml	NNP
cord-299281-5z1xminb	317	17	in	in	IN
cord-299281-5z1xminb	317	18	the	the	DT
cord-299281-5z1xminb	317	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	317	20	complex	complex	NN
cord-299281-5z1xminb	317	21	.	.	.
cord-299281-5z1xminb	318	1	An	an	DT
cord-299281-5z1xminb	318	2	unusual	unusual	JJ
cord-299281-5z1xminb	318	3	feature	feature	NN
cord-299281-5z1xminb	318	4	of	of	IN
cord-299281-5z1xminb	318	5	the	the	DT
cord-299281-5z1xminb	318	6	Gml	Gml	NNP
cord-299281-5z1xminb	318	7	oligomer	oligomer	NN
cord-299281-5z1xminb	318	8	(	(	-LRB-
cord-299281-5z1xminb	318	9	its	-PRON-	PRP$
cord-299281-5z1xminb	318	10	resistance	resistance	NN
cord-299281-5z1xminb	318	11	to	to	IN
cord-299281-5z1xminb	318	12	solubflization	solubflization	NNP
cord-299281-5z1xminb	318	13	by	by	IN
cord-299281-5z1xminb	318	14	SDS	SDS	NNP
cord-299281-5z1xminb	318	15	)	)	-RRB-
cord-299281-5z1xminb	318	16	allowed	allow	VBD
cord-299281-5z1xminb	318	17	us	-PRON-	PRP
cord-299281-5z1xminb	318	18	to	to	TO
cord-299281-5z1xminb	318	19	easily	easily	RB
cord-299281-5z1xminb	318	20	assay	assay	VB
cord-299281-5z1xminb	318	21	oligomer	oligomer	NNP
cord-299281-5z1xminb	318	22	formation	formation	NN
cord-299281-5z1xminb	318	23	.	.	.
cord-299281-5z1xminb	319	1	The	the	DT
cord-299281-5z1xminb	319	2	specificity	specificity	NN
cord-299281-5z1xminb	319	3	of	of	IN
cord-299281-5z1xminb	319	4	oligomerization	oligomerization	NN
cord-299281-5z1xminb	319	5	was	be	VBD
cord-299281-5z1xminb	319	6	mediated	mediate	VBN
cord-299281-5z1xminb	319	7	by	by	IN
cord-299281-5z1xminb	319	8	the	the	DT
cord-299281-5z1xminb	319	9	ml	ml	NN
cord-299281-5z1xminb	319	10	transmembrane	transmembrane	NN
cord-299281-5z1xminb	319	11	domain	domain	NN
cord-299281-5z1xminb	319	12	,	,	,
cord-299281-5z1xminb	319	13	since	since	IN
cord-299281-5z1xminb	319	14	mutant	mutant	JJ
cord-299281-5z1xminb	319	15	Gml	Gml	NNP
cord-299281-5z1xminb	319	16	proteins	protein	NNS
cord-299281-5z1xminb	319	17	that	that	WDT
cord-299281-5z1xminb	319	18	were	be	VBD
cord-299281-5z1xminb	319	19	retained	retain	VBN
cord-299281-5z1xminb	319	20	in	in	IN
cord-299281-5z1xminb	319	21	the	the	DT
cord-299281-5z1xminb	319	22	Golgi	Golgi	NNP
cord-299281-5z1xminb	319	23	formed	form	VBN
cord-299281-5z1xminb	319	24	oligomers	oligomer	NNS
cord-299281-5z1xminb	319	25	,	,	,
cord-299281-5z1xminb	319	26	while	while	IN
cord-299281-5z1xminb	319	27	mutant	mutant	JJ
cord-299281-5z1xminb	319	28	proteins	protein	NNS
cord-299281-5z1xminb	319	29	that	that	WDT
cord-299281-5z1xminb	319	30	were	be	VBD
cord-299281-5z1xminb	319	31	released	release	VBN
cord-299281-5z1xminb	319	32	to	to	IN
cord-299281-5z1xminb	319	33	the	the	DT
cord-299281-5z1xminb	319	34	plasma	plasma	NN
cord-299281-5z1xminb	319	35	membrane	membrane	NN
cord-299281-5z1xminb	319	36	did	do	VBD
cord-299281-5z1xminb	319	37	not	not	RB
cord-299281-5z1xminb	319	38	.	.	.
cord-299281-5z1xminb	320	1	This	this	DT
cord-299281-5z1xminb	320	2	specificity	specificity	NN
cord-299281-5z1xminb	320	3	was	be	VBD
cord-299281-5z1xminb	320	4	preserved	preserve	VBN
cord-299281-5z1xminb	320	5	in	in	IN
cord-299281-5z1xminb	320	6	another	another	DT
cord-299281-5z1xminb	320	7	construct	construct	NN
cord-299281-5z1xminb	320	8	,	,	,
cord-299281-5z1xminb	320	9	otmlG	otmlG	NNP
cord-299281-5z1xminb	320	10	,	,	,
cord-299281-5z1xminb	320	11	in	in	IN
cord-299281-5z1xminb	320	12	which	which	WDT
cord-299281-5z1xminb	320	13	the	the	DT
cord-299281-5z1xminb	320	14	lumenal	lumenal	JJ
cord-299281-5z1xminb	320	15	domain	domain	NN
cord-299281-5z1xminb	320	16	of	of	IN
cord-299281-5z1xminb	320	17	VSV	VSV	NNP
cord-299281-5z1xminb	320	18	G	G	NNP
cord-299281-5z1xminb	320	19	was	be	VBD
cord-299281-5z1xminb	320	20	replaced	replace	VBN
cord-299281-5z1xminb	320	21	with	with	IN
cord-299281-5z1xminb	320	22	the	the	DT
cord-299281-5z1xminb	320	23	o~	o~	CD
cord-299281-5z1xminb	320	24	subunit	subunit	NN
cord-299281-5z1xminb	320	25	of	of	IN
cord-299281-5z1xminb	320	26	hCG	hCG	NNP
cord-299281-5z1xminb	320	27	.	.	.
cord-299281-5z1xminb	321	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	321	2	,	,	,
cord-299281-5z1xminb	321	3	e	e	LS
cord-299281-5z1xminb	321	4	~	~	NFP
cord-299281-5z1xminb	321	5	mlG	mlG	-LRB-
cord-299281-5z1xminb	321	6	was	be	VBD
cord-299281-5z1xminb	321	7	able	able	JJ
cord-299281-5z1xminb	321	8	to	to	TO
cord-299281-5z1xminb	321	9	form	form	VB
cord-299281-5z1xminb	321	10	hetero	hetero	NNS
cord-299281-5z1xminb	321	11	-	-	NNS
cord-299281-5z1xminb	321	12	oligomers	oligomer	NNS
cord-299281-5z1xminb	321	13	with	with	IN
cord-299281-5z1xminb	321	14	Gml	Gml	NNP
cord-299281-5z1xminb	321	15	and	and	CC
cord-299281-5z1xminb	321	16	other	other	JJ
cord-299281-5z1xminb	321	17	Golgi	golgi	NN
cord-299281-5z1xminb	321	18	-	-	HYPH
cord-299281-5z1xminb	321	19	resident	resident	NN
cord-299281-5z1xminb	321	20	Gml	Gml	NNP
cord-299281-5z1xminb	321	21	mutants	mutant	NNS
cord-299281-5z1xminb	321	22	,	,	,
cord-299281-5z1xminb	321	23	but	but	CC
cord-299281-5z1xminb	321	24	not	not	RB
cord-299281-5z1xminb	321	25	with	with	IN
cord-299281-5z1xminb	321	26	mutants	mutant	NNS
cord-299281-5z1xminb	321	27	that	that	WDT
cord-299281-5z1xminb	321	28	move	move	VBP
cord-299281-5z1xminb	321	29	to	to	IN
cord-299281-5z1xminb	321	30	the	the	DT
cord-299281-5z1xminb	321	31	plasma	plasma	NN
cord-299281-5z1xminb	321	32	membrane	membrane	NN
cord-299281-5z1xminb	321	33	.	.	.
cord-299281-5z1xminb	322	1	This	this	DT
cord-299281-5z1xminb	322	2	suggested	suggest	VBD
cord-299281-5z1xminb	322	3	that	that	IN
cord-299281-5z1xminb	322	4	the	the	DT
cord-299281-5z1xminb	322	5	lumenal	lumenal	JJ
cord-299281-5z1xminb	322	6	domain	domain	NN
cord-299281-5z1xminb	322	7	of	of	IN
cord-299281-5z1xminb	322	8	VSV	VSV	NNP
cord-299281-5z1xminb	322	9	G	G	NNP
cord-299281-5z1xminb	322	10	was	be	VBD
cord-299281-5z1xminb	322	11	not	not	RB
cord-299281-5z1xminb	322	12	required	require	VBN
cord-299281-5z1xminb	322	13	for	for	IN
cord-299281-5z1xminb	322	14	oligomerization	oligomerization	NN
cord-299281-5z1xminb	322	15	.	.	.
cord-299281-5z1xminb	323	1	While	while	IN
cord-299281-5z1xminb	323	2	the	the	DT
cord-299281-5z1xminb	323	3	specificity	specificity	NN
cord-299281-5z1xminb	323	4	of	of	IN
cord-299281-5z1xminb	323	5	oligomerization	oligomerization	NN
cord-299281-5z1xminb	323	6	was	be	VBD
cord-299281-5z1xminb	323	7	dictated	dictate	VBN
cord-299281-5z1xminb	323	8	by	by	IN
cord-299281-5z1xminb	323	9	the	the	DT
cord-299281-5z1xminb	323	10	sequence	sequence	NN
cord-299281-5z1xminb	323	11	of	of	IN
cord-299281-5z1xminb	323	12	the	the	DT
cord-299281-5z1xminb	323	13	membrane	membrane	NN
cord-299281-5z1xminb	323	14	-	-	HYPH
cord-299281-5z1xminb	323	15	spanning	span	VBG
cord-299281-5z1xminb	323	16	region	region	NN
cord-299281-5z1xminb	323	17	,	,	,
cord-299281-5z1xminb	323	18	the	the	DT
cord-299281-5z1xminb	323	19	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	323	20	tail	tail	NN
cord-299281-5z1xminb	323	21	of	of	IN
cord-299281-5z1xminb	323	22	Gml	Gml	NNP
cord-299281-5z1xminb	323	23	was	be	VBD
cord-299281-5z1xminb	323	24	necessary	necessary	JJ
cord-299281-5z1xminb	323	25	for	for	IN
cord-299281-5z1xminb	323	26	SDS	sds	NN
cord-299281-5z1xminb	323	27	-	-	HYPH
cord-299281-5z1xminb	323	28	resistance	resistance	NN
cord-299281-5z1xminb	323	29	and	and	CC
cord-299281-5z1xminb	323	30	may	may	MD
cord-299281-5z1xminb	323	31	play	play	VB
cord-299281-5z1xminb	323	32	a	a	DT
cord-299281-5z1xminb	323	33	role	role	NN
cord-299281-5z1xminb	323	34	in	in	IN
cord-299281-5z1xminb	323	35	oligomer	oligomer	NNP
cord-299281-5z1xminb	323	36	formation	formation	NN
cord-299281-5z1xminb	323	37	.	.	.
cord-299281-5z1xminb	324	1	When	when	WRB
cord-299281-5z1xminb	324	2	the	the	DT
cord-299281-5z1xminb	324	3	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	324	4	tails	tail	NNS
cord-299281-5z1xminb	324	5	of	of	IN
cord-299281-5z1xminb	324	6	preexisting	preexisting	JJ
cord-299281-5z1xminb	324	7	oligomers	oligomer	NNS
cord-299281-5z1xminb	324	8	were	be	VBD
cord-299281-5z1xminb	324	9	digested	digest	VBN
cord-299281-5z1xminb	324	10	with	with	IN
cord-299281-5z1xminb	324	11	trypsin	trypsin	NN
cord-299281-5z1xminb	324	12	,	,	,
cord-299281-5z1xminb	324	13	the	the	DT
cord-299281-5z1xminb	324	14	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	324	15	products	product	NNS
cord-299281-5z1xminb	324	16	were	be	VBD
cord-299281-5z1xminb	324	17	soluble	soluble	JJ
cord-299281-5z1xminb	324	18	in	in	IN
cord-299281-5z1xminb	324	19	SDS	SDS	NNP
cord-299281-5z1xminb	324	20	but	but	CC
cord-299281-5z1xminb	324	21	continued	continue	VBD
cord-299281-5z1xminb	324	22	to	to	TO
cord-299281-5z1xminb	324	23	migrate	migrate	VB
cord-299281-5z1xminb	324	24	as	as	IN
cord-299281-5z1xminb	324	25	oligomers	oligomer	NNS
cord-299281-5z1xminb	324	26	on	on	IN
cord-299281-5z1xminb	324	27	sucrose	sucrose	NN
cord-299281-5z1xminb	324	28	gradients	gradient	NNS
cord-299281-5z1xminb	324	29	.	.	.
cord-299281-5z1xminb	325	1	Based	base	VBN
cord-299281-5z1xminb	325	2	on	on	IN
cord-299281-5z1xminb	325	3	potential	potential	JJ
cord-299281-5z1xminb	325	4	trypsin	trypsin	NN
cord-299281-5z1xminb	325	5	cleavage	cleavage	NN
cord-299281-5z1xminb	325	6	sites	site	NNS
cord-299281-5z1xminb	325	7	in	in	IN
cord-299281-5z1xminb	325	8	the	the	DT
cord-299281-5z1xminb	325	9	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	325	10	tail	tail	NN
cord-299281-5z1xminb	325	11	of	of	IN
cord-299281-5z1xminb	325	12	Gml	Gml	NNP
cord-299281-5z1xminb	325	13	and	and	CC
cord-299281-5z1xminb	325	14	the	the	DT
cord-299281-5z1xminb	325	15	migration	migration	NN
cord-299281-5z1xminb	325	16	of	of	IN
cord-299281-5z1xminb	325	17	the	the	DT
cord-299281-5z1xminb	325	18	proteolytic	proteolytic	JJ
cord-299281-5z1xminb	325	19	fragments	fragment	NNS
cord-299281-5z1xminb	325	20	on	on	IN
cord-299281-5z1xminb	325	21	SDS	SDS	NNP
cord-299281-5z1xminb	325	22	-	-	HYPH
cord-299281-5z1xminb	325	23	PAGE	PAGE	NNP
cord-299281-5z1xminb	325	24	,	,	,
cord-299281-5z1xminb	325	25	it	-PRON-	PRP
cord-299281-5z1xminb	325	26	is	be	VBZ
cord-299281-5z1xminb	325	27	possible	possible	JJ
cord-299281-5z1xminb	325	28	that	that	IN
cord-299281-5z1xminb	325	29	removal	removal	NN
cord-299281-5z1xminb	325	30	of	of	IN
cord-299281-5z1xminb	325	31	the	the	DT
cord-299281-5z1xminb	325	32	last	last	JJ
cord-299281-5z1xminb	325	33	three	three	CD
cord-299281-5z1xminb	325	34	carboxyterminal	carboxyterminal	JJ
cord-299281-5z1xminb	325	35	residues	residue	NNS
cord-299281-5z1xminb	325	36	of	of	IN
cord-299281-5z1xminb	325	37	Gml	Gml	NNP
cord-299281-5z1xminb	325	38	destroys	destroy	VBZ
cord-299281-5z1xminb	325	39	its	-PRON-	PRP$
cord-299281-5z1xminb	325	40	resistance	resistance	NN
cord-299281-5z1xminb	325	41	to	to	IN
cord-299281-5z1xminb	325	42	solubilization	solubilization	NN
cord-299281-5z1xminb	325	43	by	by	IN
cord-299281-5z1xminb	325	44	SDS	SDS	NNP
cord-299281-5z1xminb	325	45	.	.	.
cord-299281-5z1xminb	326	1	Alternatively	alternatively	RB
cord-299281-5z1xminb	326	2	,	,	,
cord-299281-5z1xminb	326	3	trypsin	trypsin	JJ
cord-299281-5z1xminb	326	4	digestion	digestion	NN
cord-299281-5z1xminb	326	5	could	could	MD
cord-299281-5z1xminb	326	6	remove	remove	VB
cord-299281-5z1xminb	326	7	another	another	DT
cord-299281-5z1xminb	326	8	component	component	NN
cord-299281-5z1xminb	326	9	of	of	IN
cord-299281-5z1xminb	326	10	the	the	DT
cord-299281-5z1xminb	326	11	oligomer	oligomer	NN
cord-299281-5z1xminb	326	12	that	that	WDT
cord-299281-5z1xminb	326	13	is	be	VBZ
cord-299281-5z1xminb	326	14	required	require	VBN
cord-299281-5z1xminb	326	15	for	for	IN
cord-299281-5z1xminb	326	16	SDS	SDS	NNP
cord-299281-5z1xminb	326	17	resistance	resistance	NN
cord-299281-5z1xminb	326	18	.	.	.
cord-299281-5z1xminb	327	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	327	2	,	,	,
cord-299281-5z1xminb	327	3	cell	cell	NN
cord-299281-5z1xminb	327	4	treatment	treatment	NN
cord-299281-5z1xminb	327	5	with	with	IN
cord-299281-5z1xminb	327	6	cytochalasin	cytochalasin	NNP
cord-299281-5z1xminb	327	7	D	D	NNP
cord-299281-5z1xminb	327	8	blocked	block	VBD
cord-299281-5z1xminb	327	9	the	the	DT
cord-299281-5z1xminb	327	10	formation	formation	NN
cord-299281-5z1xminb	327	11	of	of	IN
cord-299281-5z1xminb	327	12	SDS	sds	NN
cord-299281-5z1xminb	327	13	-	-	HYPH
cord-299281-5z1xminb	327	14	resistant	resistant	JJ
cord-299281-5z1xminb	327	15	oligomers	oligomer	NNS
cord-299281-5z1xminb	327	16	,	,	,
cord-299281-5z1xminb	327	17	suggesting	suggest	VBG
cord-299281-5z1xminb	327	18	that	that	IN
cord-299281-5z1xminb	327	19	the	the	DT
cord-299281-5z1xminb	327	20	acquisition	acquisition	NN
cord-299281-5z1xminb	327	21	of	of	IN
cord-299281-5z1xminb	327	22	SDS	SDS	NNP
cord-299281-5z1xminb	327	23	resistance	resistance	NN
cord-299281-5z1xminb	327	24	may	may	MD
cord-299281-5z1xminb	327	25	reflect	reflect	VB
cord-299281-5z1xminb	327	26	association	association	NN
cord-299281-5z1xminb	327	27	of	of	IN
cord-299281-5z1xminb	327	28	the	the	DT
cord-299281-5z1xminb	327	29	Gml	Gml	NNP
cord-299281-5z1xminb	327	30	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	327	31	tail	tail	NN
cord-299281-5z1xminb	327	32	with	with	IN
cord-299281-5z1xminb	327	33	an	an	DT
cord-299281-5z1xminb	327	34	actin	actin	NN
cord-299281-5z1xminb	327	35	-	-	HYPH
cord-299281-5z1xminb	327	36	based	base	VBN
cord-299281-5z1xminb	327	37	cytoskeleton	cytoskeleton	NN
cord-299281-5z1xminb	327	38	.	.	.
cord-299281-5z1xminb	328	1	We	-PRON-	PRP
cord-299281-5z1xminb	328	2	are	be	VBP
cord-299281-5z1xminb	328	3	currently	currently	RB
cord-299281-5z1xminb	328	4	constructing	construct	VBG
cord-299281-5z1xminb	328	5	a	a	DT
cord-299281-5z1xminb	328	6	series	series	NN
cord-299281-5z1xminb	328	7	of	of	IN
cord-299281-5z1xminb	328	8	Gml	Gml	NNP
cord-299281-5z1xminb	328	9	proteins	protein	NNS
cord-299281-5z1xminb	328	10	with	with	IN
cord-299281-5z1xminb	328	11	truncated	truncated	JJ
cord-299281-5z1xminb	328	12	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	328	13	tails	tail	NNS
cord-299281-5z1xminb	328	14	to	to	TO
cord-299281-5z1xminb	328	15	determine	determine	VB
cord-299281-5z1xminb	328	16	if	if	IN
cord-299281-5z1xminb	328	17	these	these	DT
cord-299281-5z1xminb	328	18	proteins	protein	NNS
cord-299281-5z1xminb	328	19	are	be	VBP
cord-299281-5z1xminb	328	20	correctly	correctly	RB
cord-299281-5z1xminb	328	21	targeted	target	VBN
cord-299281-5z1xminb	328	22	and	and	CC
cord-299281-5z1xminb	328	23	form	form	VB
cord-299281-5z1xminb	328	24	oligomers	oligomer	NNS
cord-299281-5z1xminb	328	25	.	.	.
cord-299281-5z1xminb	329	1	Several	several	JJ
cord-299281-5z1xminb	329	2	observations	observation	NNS
cord-299281-5z1xminb	329	3	suggest	suggest	VBP
cord-299281-5z1xminb	329	4	that	that	IN
cord-299281-5z1xminb	329	5	Gml	Gml	NNP
cord-299281-5z1xminb	329	6	oligomerization	oligomerization	NN
cord-299281-5z1xminb	329	7	normally	normally	RB
cord-299281-5z1xminb	329	8	occurs	occur	VBZ
cord-299281-5z1xminb	329	9	after	after	IN
cord-299281-5z1xminb	329	10	the	the	DT
cord-299281-5z1xminb	329	11	protein	protein	NN
cord-299281-5z1xminb	329	12	leaves	leave	VBZ
cord-299281-5z1xminb	329	13	the	the	DT
cord-299281-5z1xminb	329	14	ER	ER	NNP
cord-299281-5z1xminb	329	15	.	.	.
cord-299281-5z1xminb	330	1	Oligomerization	Oligomerization	NNP
cord-299281-5z1xminb	330	2	of	of	IN
cord-299281-5z1xminb	330	3	Gml	Gml	NNP
cord-299281-5z1xminb	330	4	was	be	VBD
cord-299281-5z1xminb	330	5	not	not	RB
cord-299281-5z1xminb	330	6	detected	detect	VBN
cord-299281-5z1xminb	330	7	until	until	IN
cord-299281-5z1xminb	330	8	~10	~10	NNP
cord-299281-5z1xminb	330	9	min	min	NN
cord-299281-5z1xminb	330	10	after	after	IN
cord-299281-5z1xminb	330	11	protein	protein	NN
cord-299281-5z1xminb	330	12	synthesis	synthesis	NN
cord-299281-5z1xminb	330	13	,	,	,
cord-299281-5z1xminb	330	14	which	which	WDT
cord-299281-5z1xminb	330	15	correlates	correlate	VBZ
cord-299281-5z1xminb	330	16	with	with	IN
cord-299281-5z1xminb	330	17	arrival	arrival	NN
cord-299281-5z1xminb	330	18	of	of	IN
cord-299281-5z1xminb	330	19	newly	newly	RB
cord-299281-5z1xminb	330	20	synthesized	synthesize	VBN
cord-299281-5z1xminb	330	21	proteins	protein	NNS
cord-299281-5z1xminb	330	22	at	at	IN
cord-299281-5z1xminb	330	23	the	the	DT
cord-299281-5z1xminb	330	24	cis	cis	NNP
cord-299281-5z1xminb	330	25	-	-	HYPH
cord-299281-5z1xminb	330	26	Golgi	Golgi	NNP
cord-299281-5z1xminb	330	27	.	.	.
cord-299281-5z1xminb	331	1	Oligomers	oligomer	NNS
cord-299281-5z1xminb	331	2	were	be	VBD
cord-299281-5z1xminb	331	3	not	not	RB
cord-299281-5z1xminb	331	4	observed	observe	VBN
cord-299281-5z1xminb	331	5	when	when	WRB
cord-299281-5z1xminb	331	6	ER	ER	NNP
cord-299281-5z1xminb	331	7	to	to	IN
cord-299281-5z1xminb	331	8	Golgi	Golgi	NNP
cord-299281-5z1xminb	331	9	traffic	traffic	NN
cord-299281-5z1xminb	331	10	was	be	VBD
cord-299281-5z1xminb	331	11	blocked	block	VBN
cord-299281-5z1xminb	331	12	by	by	IN
cord-299281-5z1xminb	331	13	incubation	incubation	NN
cord-299281-5z1xminb	331	14	at	at	IN
cord-299281-5z1xminb	331	15	16	16	CD
cord-299281-5z1xminb	331	16	°	°	NN
cord-299281-5z1xminb	331	17	C	c	NN
cord-299281-5z1xminb	331	18	or	or	CC
cord-299281-5z1xminb	331	19	treatment	treatment	NN
cord-299281-5z1xminb	331	20	with	with	IN
cord-299281-5z1xminb	331	21	CCCP	CCCP	NNP
cord-299281-5z1xminb	331	22	.	.	.
cord-299281-5z1xminb	332	1	Interestingly	interestingly	RB
cord-299281-5z1xminb	332	2	,	,	,
cord-299281-5z1xminb	332	3	SDS	sds	NN
cord-299281-5z1xminb	332	4	-	-	HYPH
cord-299281-5z1xminb	332	5	resistant	resistant	JJ
cord-299281-5z1xminb	332	6	oligomers	oligomer	NNS
cord-299281-5z1xminb	332	7	did	do	VBD
cord-299281-5z1xminb	332	8	form	form	NN
cord-299281-5z1xminb	332	9	in	in	IN
cord-299281-5z1xminb	332	10	brefeldin	brefeldin	NNP
cord-299281-5z1xminb	332	11	A	A	NNP
cord-299281-5z1xminb	332	12	-	-	HYPH
cord-299281-5z1xminb	332	13	treated	treat	VBN
cord-299281-5z1xminb	332	14	cells	cell	NNS
cord-299281-5z1xminb	332	15	,	,	,
cord-299281-5z1xminb	332	16	suggesting	suggest	VBG
cord-299281-5z1xminb	332	17	that	that	IN
cord-299281-5z1xminb	332	18	oligomers	oligomer	NNS
cord-299281-5z1xminb	332	19	can	can	MD
cord-299281-5z1xminb	332	20	form	form	VB
cord-299281-5z1xminb	332	21	in	in	IN
cord-299281-5z1xminb	332	22	pre	pre	JJ
cord-299281-5z1xminb	332	23	-	-	JJ
cord-299281-5z1xminb	332	24	Golgi	golgi	JJ
cord-299281-5z1xminb	332	25	compartments	compartment	NNS
cord-299281-5z1xminb	332	26	under	under	IN
cord-299281-5z1xminb	332	27	certain	certain	JJ
cord-299281-5z1xminb	332	28	conditions	condition	NNS
cord-299281-5z1xminb	332	29	.	.	.
cord-299281-5z1xminb	333	1	Brefeldin	Brefeldin	NNP
cord-299281-5z1xminb	333	2	A	a	DT
cord-299281-5z1xminb	333	3	treatment	treatment	NN
cord-299281-5z1xminb	333	4	may	may	MD
cord-299281-5z1xminb	333	5	generate	generate	VB
cord-299281-5z1xminb	333	6	the	the	DT
cord-299281-5z1xminb	333	7	conditions	condition	NNS
cord-299281-5z1xminb	333	8	required	require	VBN
cord-299281-5z1xminb	333	9	to	to	TO
cord-299281-5z1xminb	333	10	stimulate	stimulate	VB
cord-299281-5z1xminb	333	11	oligomer	oligomer	NNP
cord-299281-5z1xminb	333	12	formation	formation	NN
cord-299281-5z1xminb	333	13	in	in	IN
cord-299281-5z1xminb	333	14	the	the	DT
cord-299281-5z1xminb	333	15	ER	ER	NNP
cord-299281-5z1xminb	333	16	.	.	.
cord-299281-5z1xminb	334	1	Conditions	condition	NNS
cord-299281-5z1xminb	334	2	which	which	WDT
cord-299281-5z1xminb	334	3	could	could	MD
cord-299281-5z1xminb	334	4	trigger	trigger	VB
cord-299281-5z1xminb	334	5	Gml	Gml	NNP
cord-299281-5z1xminb	334	6	oligomerization	oligomerization	NN
cord-299281-5z1xminb	334	7	might	may	MD
cord-299281-5z1xminb	334	8	include	include	VB
cord-299281-5z1xminb	334	9	its	-PRON-	PRP$
cord-299281-5z1xminb	334	10	concentration	concentration	NN
cord-299281-5z1xminb	334	11	in	in	IN
cord-299281-5z1xminb	334	12	the	the	DT
cord-299281-5z1xminb	334	13	membrane	membrane	NN
cord-299281-5z1xminb	334	14	,	,	,
cord-299281-5z1xminb	334	15	the	the	DT
cord-299281-5z1xminb	334	16	retrieval	retrieval	NN
cord-299281-5z1xminb	334	17	of	of	IN
cord-299281-5z1xminb	334	18	glycolipids	glycolipid	NNS
cord-299281-5z1xminb	334	19	or	or	CC
cord-299281-5z1xminb	334	20	glycoproteins	glycoprotein	NNS
cord-299281-5z1xminb	334	21	typical	typical	JJ
cord-299281-5z1xminb	334	22	of	of	IN
cord-299281-5z1xminb	334	23	the	the	DT
cord-299281-5z1xminb	334	24	Golgi	Golgi	NNP
cord-299281-5z1xminb	334	25	complex	complex	NN
cord-299281-5z1xminb	334	26	,	,	,
cord-299281-5z1xminb	334	27	or	or	CC
cord-299281-5z1xminb	334	28	changes	change	NNS
cord-299281-5z1xminb	334	29	in	in	IN
cord-299281-5z1xminb	334	30	pH	pH	NNP
cord-299281-5z1xminb	334	31	or	or	CC
cord-299281-5z1xminb	334	32	divalent	divalent	JJ
cord-299281-5z1xminb	334	33	ion	ion	NN
cord-299281-5z1xminb	334	34	concentrations	concentration	NNS
cord-299281-5z1xminb	334	35	.	.	.
cord-299281-5z1xminb	335	1	We	-PRON-	PRP
cord-299281-5z1xminb	335	2	were	be	VBD
cord-299281-5z1xminb	335	3	unable	unable	JJ
cord-299281-5z1xminb	335	4	to	to	TO
cord-299281-5z1xminb	335	5	solubilize	solubilize	VB
cord-299281-5z1xminb	335	6	the	the	DT
cord-299281-5z1xminb	335	7	SDS	SDS	NNP
cord-299281-5z1xminb	335	8	-	-	HYPH
cord-299281-5z1xminb	335	9	resistant	resistant	JJ
cord-299281-5z1xminb	335	10	oligomer	oligomer	NNP
cord-299281-5z1xminb	335	11	using	use	VBG
cord-299281-5z1xminb	335	12	a	a	DT
cord-299281-5z1xminb	335	13	wide	wide	JJ
cord-299281-5z1xminb	335	14	variety	variety	NN
cord-299281-5z1xminb	335	15	of	of	IN
cord-299281-5z1xminb	335	16	harsh	harsh	JJ
cord-299281-5z1xminb	335	17	conditions	condition	NNS
cord-299281-5z1xminb	335	18	.	.	.
cord-299281-5z1xminb	336	1	It	-PRON-	PRP
cord-299281-5z1xminb	336	2	is	be	VBZ
cord-299281-5z1xminb	336	3	possible	possible	JJ
cord-299281-5z1xminb	336	4	that	that	IN
cord-299281-5z1xminb	336	5	some	some	DT
cord-299281-5z1xminb	336	6	of	of	IN
cord-299281-5z1xminb	336	7	the	the	DT
cord-299281-5z1xminb	336	8	Gml	Gml	NNP
cord-299281-5z1xminb	336	9	oligomer	oligomer	NN
cord-299281-5z1xminb	336	10	is	be	VBZ
cord-299281-5z1xminb	336	11	covalently	covalently	RB
cord-299281-5z1xminb	336	12	crosslinked	crosslinked	JJ
cord-299281-5z1xminb	336	13	to	to	IN
cord-299281-5z1xminb	336	14	itself	-PRON-	PRP
cord-299281-5z1xminb	336	15	or	or	CC
cord-299281-5z1xminb	336	16	to	to	IN
cord-299281-5z1xminb	336	17	an	an	DT
cord-299281-5z1xminb	336	18	actin	actin	NN
cord-299281-5z1xminb	336	19	-	-	HYPH
cord-299281-5z1xminb	336	20	based	base	VBN
cord-299281-5z1xminb	336	21	structure	structure	NN
cord-299281-5z1xminb	336	22	via	via	IN
cord-299281-5z1xminb	336	23	the	the	DT
cord-299281-5z1xminb	336	24	VSV	VSV	NNP
cord-299281-5z1xminb	336	25	G	G	NNP
cord-299281-5z1xminb	336	26	tail	tail	NN
cord-299281-5z1xminb	336	27	.	.	.
cord-299281-5z1xminb	337	1	However	however	RB
cord-299281-5z1xminb	337	2	,	,	,
cord-299281-5z1xminb	337	3	removal	removal	NN
cord-299281-5z1xminb	337	4	of	of	IN
cord-299281-5z1xminb	337	5	as	as	RB
cord-299281-5z1xminb	337	6	few	few	JJ
cord-299281-5z1xminb	337	7	as	as	IN
cord-299281-5z1xminb	337	8	three	three	CD
cord-299281-5z1xminb	337	9	amino	amino	NN
cord-299281-5z1xminb	337	10	acids	acid	NNS
cord-299281-5z1xminb	337	11	from	from	IN
cord-299281-5z1xminb	337	12	the	the	DT
cord-299281-5z1xminb	337	13	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	337	14	tail	tail	NN
cord-299281-5z1xminb	337	15	resulted	result	VBD
cord-299281-5z1xminb	337	16	in	in	IN
cord-299281-5z1xminb	337	17	oligomers	oligomer	NNS
cord-299281-5z1xminb	337	18	that	that	WDT
cord-299281-5z1xminb	337	19	were	be	VBD
cord-299281-5z1xminb	337	20	SDS	SDS	NNP
cord-299281-5z1xminb	337	21	-	-	HYPH
cord-299281-5z1xminb	337	22	sensitive	sensitive	JJ
cord-299281-5z1xminb	337	23	.	.	.
cord-299281-5z1xminb	338	1	This	this	DT
cord-299281-5z1xminb	338	2	indicates	indicate	VBZ
cord-299281-5z1xminb	338	3	that	that	IN
cord-299281-5z1xminb	338	4	the	the	DT
cord-299281-5z1xminb	338	5	SDS	SDS	NNP
cord-299281-5z1xminb	338	6	resistant	resistant	JJ
cord-299281-5z1xminb	338	7	phenotype	phenotype	NN
cord-299281-5z1xminb	338	8	(	(	-LRB-
cord-299281-5z1xminb	338	9	which	which	WDT
cord-299281-5z1xminb	338	10	requires	require	VBZ
cord-299281-5z1xminb	338	11	the	the	DT
cord-299281-5z1xminb	338	12	last	last	JJ
cord-299281-5z1xminb	338	13	three	three	CD
cord-299281-5z1xminb	338	14	amino	amino	NN
cord-299281-5z1xminb	338	15	acids	acid	NNS
cord-299281-5z1xminb	338	16	of	of	IN
cord-299281-5z1xminb	338	17	Gml	Gml	NNP
cord-299281-5z1xminb	338	18	)	)	-RRB-
cord-299281-5z1xminb	338	19	can	can	MD
cord-299281-5z1xminb	338	20	be	be	VB
cord-299281-5z1xminb	338	21	separated	separate	VBN
cord-299281-5z1xminb	338	22	from	from	IN
cord-299281-5z1xminb	338	23	oligomerization	oligomerization	NN
cord-299281-5z1xminb	338	24	per	per	FW
cord-299281-5z1xminb	338	25	se	se	FW
cord-299281-5z1xminb	338	26	,	,	,
cord-299281-5z1xminb	338	27	which	which	WDT
cord-299281-5z1xminb	338	28	is	be	VBZ
cord-299281-5z1xminb	338	29	mediated	mediate	VBN
cord-299281-5z1xminb	338	30	by	by	IN
cord-299281-5z1xminb	338	31	the	the	DT
cord-299281-5z1xminb	338	32	membranespanning	membranespanne	VBG
cord-299281-5z1xminb	338	33	domain	domain	NN
cord-299281-5z1xminb	338	34	.	.	.
cord-299281-5z1xminb	339	1	The	the	DT
cord-299281-5z1xminb	339	2	transmembrane	transmembrane	NN
cord-299281-5z1xminb	339	3	domains	domain	NNS
cord-299281-5z1xminb	339	4	of	of	IN
cord-299281-5z1xminb	339	5	many	many	JJ
cord-299281-5z1xminb	339	6	proteins	protein	NNS
cord-299281-5z1xminb	339	7	have	have	VBP
cord-299281-5z1xminb	339	8	been	be	VBN
cord-299281-5z1xminb	339	9	shown	show	VBN
cord-299281-5z1xminb	339	10	to	to	TO
cord-299281-5z1xminb	339	11	mediate	mediate	VB
cord-299281-5z1xminb	339	12	dimerization	dimerization	NN
cord-299281-5z1xminb	339	13	(	(	-LRB-
cord-299281-5z1xminb	339	14	Sternberg	Sternberg	NNP
cord-299281-5z1xminb	339	15	and	and	CC
cord-299281-5z1xminb	339	16	Gullick	Gullick	NNP
cord-299281-5z1xminb	339	17	,	,	,
cord-299281-5z1xminb	339	18	1990	1990	CD
cord-299281-5z1xminb	339	19	;	;	:
cord-299281-5z1xminb	340	1	Manolios	Manolios	NNP
cord-299281-5z1xminb	340	2	et	et	FW
cord-299281-5z1xminb	340	3	al	al	NNP
cord-299281-5z1xminb	340	4	.	.	.
cord-299281-5z1xminb	341	1	,	,	,
cord-299281-5z1xminb	341	2	1990	1990	CD
cord-299281-5z1xminb	341	3	;	;	:
cord-299281-5z1xminb	341	4	for	for	IN
cord-299281-5z1xminb	341	5	review	review	NN
cord-299281-5z1xminb	341	6	see	see	VBP
cord-299281-5z1xminb	341	7	Bormann	Bormann	NNP
cord-299281-5z1xminb	341	8	and	and	CC
cord-299281-5z1xminb	341	9	Engelman	Engelman	NNP
cord-299281-5z1xminb	341	10	,	,	,
cord-299281-5z1xminb	341	11	1992	1992	CD
cord-299281-5z1xminb	341	12	)	)	-RRB-
cord-299281-5z1xminb	341	13	.	.	.
cord-299281-5z1xminb	342	1	The	the	DT
cord-299281-5z1xminb	342	2	best	good	JJS
cord-299281-5z1xminb	342	3	example	example	NN
cord-299281-5z1xminb	342	4	is	be	VBZ
cord-299281-5z1xminb	342	5	glycophorin	glycophorin	NNP
cord-299281-5z1xminb	342	6	A	A	NNP
cord-299281-5z1xminb	342	7	,	,	,
cord-299281-5z1xminb	342	8	whose	whose	WP$
cord-299281-5z1xminb	342	9	single	single	JJ
cord-299281-5z1xminb	342	10	transmembrane	transmembrane	NN
cord-299281-5z1xminb	342	11	domain	domain	NN
cord-299281-5z1xminb	342	12	interacts	interact	VBZ
cord-299281-5z1xminb	342	13	to	to	TO
cord-299281-5z1xminb	342	14	form	form	VB
cord-299281-5z1xminb	342	15	homodimers	homodimer	NNS
cord-299281-5z1xminb	343	1	(	(	-LRB-
cord-299281-5z1xminb	343	2	Bormann	Bormann	NNP
cord-299281-5z1xminb	343	3	et	et	NNP
cord-299281-5z1xminb	343	4	al	al	NNP
cord-299281-5z1xminb	343	5	.	.	NNP
cord-299281-5z1xminb	343	6	,	,	,
cord-299281-5z1xminb	343	7	1989	1989	CD
cord-299281-5z1xminb	343	8	)	)	-RRB-
cord-299281-5z1xminb	343	9	.	.	.
cord-299281-5z1xminb	344	1	This	this	DT
cord-299281-5z1xminb	344	2	dimer	dimer	NN
cord-299281-5z1xminb	344	3	is	be	VBZ
cord-299281-5z1xminb	344	4	resistant	resistant	JJ
cord-299281-5z1xminb	344	5	to	to	IN
cord-299281-5z1xminb	344	6	solubilization	solubilization	NN
cord-299281-5z1xminb	344	7	by	by	IN
cord-299281-5z1xminb	344	8	SDS	SDS	NNP
cord-299281-5z1xminb	344	9	at	at	IN
cord-299281-5z1xminb	344	10	ambient	ambient	JJ
cord-299281-5z1xminb	344	11	temperature	temperature	NN
cord-299281-5z1xminb	344	12	,	,	,
cord-299281-5z1xminb	344	13	although	although	IN
cord-299281-5z1xminb	344	14	resistance	resistance	NN
cord-299281-5z1xminb	344	15	is	be	VBZ
cord-299281-5z1xminb	344	16	overcome	overcome	VBN
cord-299281-5z1xminb	344	17	by	by	IN
cord-299281-5z1xminb	344	18	dilution	dilution	NN
cord-299281-5z1xminb	344	19	or	or	CC
cord-299281-5z1xminb	344	20	incubation	incubation	NN
cord-299281-5z1xminb	344	21	at	at	IN
cord-299281-5z1xminb	344	22	higher	high	JJR
cord-299281-5z1xminb	344	23	temperatures	temperature	NNS
cord-299281-5z1xminb	344	24	(	(	-LRB-
cord-299281-5z1xminb	344	25	Furthmayr	Furthmayr	NNP
cord-299281-5z1xminb	344	26	and	and	CC
cord-299281-5z1xminb	344	27	Marchesi	Marchesi	NNP
cord-299281-5z1xminb	344	28	,	,	,
cord-299281-5z1xminb	344	29	1976	1976	CD
cord-299281-5z1xminb	344	30	)	)	-RRB-
cord-299281-5z1xminb	344	31	.	.	.
cord-299281-5z1xminb	345	1	Dimerization	Dimerization	NNP
cord-299281-5z1xminb	345	2	ofglycophorin	ofglycophorin	NNP
cord-299281-5z1xminb	345	3	A	A	NNP
cord-299281-5z1xminb	345	4	is	be	VBZ
cord-299281-5z1xminb	345	5	highly	highly	RB
cord-299281-5z1xminb	345	6	sequence	sequence	NN
cord-299281-5z1xminb	345	7	specific	specific	JJ
cord-299281-5z1xminb	345	8	and	and	CC
cord-299281-5z1xminb	345	9	appears	appear	VBZ
cord-299281-5z1xminb	345	10	to	to	TO
cord-299281-5z1xminb	345	11	involve	involve	VB
cord-299281-5z1xminb	345	12	interactions	interaction	NNS
cord-299281-5z1xminb	345	13	between	between	IN
cord-299281-5z1xminb	345	14	hydrophobic	hydrophobic	JJ
cord-299281-5z1xminb	345	15	residues	residue	NNS
cord-299281-5z1xminb	345	16	in	in	IN
cord-299281-5z1xminb	345	17	the	the	DT
cord-299281-5z1xminb	345	18	transmembrane	transmembrane	NN
cord-299281-5z1xminb	345	19	domain	domain	NN
cord-299281-5z1xminb	345	20	(	(	-LRB-
cord-299281-5z1xminb	345	21	Lemmon	Lemmon	NNP
cord-299281-5z1xminb	345	22	et	et	NNP
cord-299281-5z1xminb	345	23	al	al	NNP
cord-299281-5z1xminb	345	24	.	.	NNP
cord-299281-5z1xminb	345	25	,	,	,
cord-299281-5z1xminb	345	26	1992	1992	CD
cord-299281-5z1xminb	345	27	)	)	-RRB-
cord-299281-5z1xminb	345	28	.	.	.
cord-299281-5z1xminb	346	1	The	the	DT
cord-299281-5z1xminb	346	2	transmembrane	transmembrane	NN
cord-299281-5z1xminb	346	3	domains	domain	NNS
cord-299281-5z1xminb	346	4	of	of	IN
cord-299281-5z1xminb	346	5	the	the	DT
cord-299281-5z1xminb	346	6	T	t	NN
cord-299281-5z1xminb	346	7	-	-	HYPH
cord-299281-5z1xminb	346	8	cell	cell	NN
cord-299281-5z1xminb	346	9	receptor	receptor	NN
cord-299281-5z1xminb	346	10	(	(	-LRB-
cord-299281-5z1xminb	346	11	Cosson	Cosson	NNP
cord-299281-5z1xminb	346	12	et	et	NNP
cord-299281-5z1xminb	346	13	al	al	NNP
cord-299281-5z1xminb	346	14	.	.	NNP
cord-299281-5z1xminb	346	15	,	,	,
cord-299281-5z1xminb	346	16	1991	1991	CD
cord-299281-5z1xminb	346	17	)	)	-RRB-
cord-299281-5z1xminb	346	18	and	and	CC
cord-299281-5z1xminb	346	19	of	of	IN
cord-299281-5z1xminb	346	20	class	class	NN
cord-299281-5z1xminb	346	21	H	h	NN
cord-299281-5z1xminb	346	22	major	major	JJ
cord-299281-5z1xminb	346	23	histocompatibility	histocompatibility	NN
cord-299281-5z1xminb	346	24	complex	complex	NN
cord-299281-5z1xminb	346	25	molecules	molecule	NNS
cord-299281-5z1xminb	346	26	also	also	RB
cord-299281-5z1xminb	346	27	appear	appear	VBP
cord-299281-5z1xminb	346	28	to	to	TO
cord-299281-5z1xminb	346	29	mediate	mediate	VB
cord-299281-5z1xminb	346	30	dimer	dimer	JJ
cord-299281-5z1xminb	346	31	formation	formation	NN
cord-299281-5z1xminb	346	32	(	(	-LRB-
cord-299281-5z1xminb	346	33	Cosson	Cosson	NNP
cord-299281-5z1xminb	346	34	and	and	CC
cord-299281-5z1xminb	346	35	Bonifacino	Bonifacino	NNP
cord-299281-5z1xminb	346	36	,	,	,
cord-299281-5z1xminb	346	37	1992	1992	CD
cord-299281-5z1xminb	346	38	)	)	-RRB-
cord-299281-5z1xminb	346	39	.	.	.
cord-299281-5z1xminb	347	1	In	in	IN
cord-299281-5z1xminb	347	2	the	the	DT
cord-299281-5z1xminb	347	3	case	case	NN
cord-299281-5z1xminb	347	4	of	of	IN
cord-299281-5z1xminb	347	5	major	major	JJ
cord-299281-5z1xminb	347	6	histocompatibility	histocompatibility	NN
cord-299281-5z1xminb	347	7	complex	complex	NN
cord-299281-5z1xminb	347	8	molecules	molecule	NNS
cord-299281-5z1xminb	347	9	,	,	,
cord-299281-5z1xminb	347	10	o~	o~	XX
cord-299281-5z1xminb	347	11	and/~	and/~	,
cord-299281-5z1xminb	347	12	chains	chain	NNS
cord-299281-5z1xminb	347	13	interact	interact	VBP
cord-299281-5z1xminb	347	14	to	to	TO
cord-299281-5z1xminb	347	15	form	form	VB
cord-299281-5z1xminb	347	16	heterodimers	heterodimer	NNS
cord-299281-5z1xminb	347	17	;	;	:
cord-299281-5z1xminb	347	18	dimerization	dimerization	NN
cord-299281-5z1xminb	347	19	appears	appear	VBZ
cord-299281-5z1xminb	347	20	to	to	TO
cord-299281-5z1xminb	347	21	require	require	VB
cord-299281-5z1xminb	347	22	a	a	DT
cord-299281-5z1xminb	347	23	pair	pair	NN
cord-299281-5z1xminb	347	24	of	of	IN
cord-299281-5z1xminb	347	25	oppositely	oppositely	RB
cord-299281-5z1xminb	347	26	charged	charge	VBN
cord-299281-5z1xminb	347	27	amino	amino	NN
cord-299281-5z1xminb	347	28	acids	acid	NNS
cord-299281-5z1xminb	347	29	at	at	IN
cord-299281-5z1xminb	347	30	the	the	DT
cord-299281-5z1xminb	347	31	lumenal	lumenal	JJ
cord-299281-5z1xminb	347	32	edge	edge	NN
cord-299281-5z1xminb	347	33	of	of	IN
cord-299281-5z1xminb	347	34	the	the	DT
cord-299281-5z1xminb	347	35	apposing	appose	VBG
cord-299281-5z1xminb	347	36	membrane	membrane	NN
cord-299281-5z1xminb	347	37	spans	span	NNS
cord-299281-5z1xminb	347	38	.	.	.
cord-299281-5z1xminb	348	1	A	a	DT
cord-299281-5z1xminb	348	2	series	series	NN
cord-299281-5z1xminb	348	3	of	of	IN
cord-299281-5z1xminb	348	4	glycine	glycine	NN
cord-299281-5z1xminb	348	5	residues	residue	NNS
cord-299281-5z1xminb	348	6	on	on	IN
cord-299281-5z1xminb	348	7	each	each	DT
cord-299281-5z1xminb	348	8	membrane	membrane	NN
cord-299281-5z1xminb	348	9	span	span	NN
cord-299281-5z1xminb	348	10	forms	form	VBZ
cord-299281-5z1xminb	348	11	a	a	DT
cord-299281-5z1xminb	348	12	nonpolar	nonpolar	JJ
cord-299281-5z1xminb	348	13	face	face	NN
cord-299281-5z1xminb	348	14	which	which	WDT
cord-299281-5z1xminb	348	15	allows	allow	VBZ
cord-299281-5z1xminb	348	16	close	close	JJ
cord-299281-5z1xminb	348	17	packing	packing	NN
cord-299281-5z1xminb	348	18	of	of	IN
cord-299281-5z1xminb	348	19	the	the	DT
cord-299281-5z1xminb	348	20	transmembrane	transmembrane	NN
cord-299281-5z1xminb	348	21	domains	domain	NNS
cord-299281-5z1xminb	348	22	(	(	-LRB-
cord-299281-5z1xminb	348	23	Cosson	Cosson	NNP
cord-299281-5z1xminb	348	24	and	and	CC
cord-299281-5z1xminb	348	25	Bonifacino	Bonifacino	NNP
cord-299281-5z1xminb	348	26	,	,	,
cord-299281-5z1xminb	348	27	1992	1992	CD
cord-299281-5z1xminb	348	28	)	)	-RRB-
cord-299281-5z1xminb	348	29	.	.	.
cord-299281-5z1xminb	349	1	Similarly	similarly	RB
cord-299281-5z1xminb	349	2	,	,	,
cord-299281-5z1xminb	349	3	the	the	DT
cord-299281-5z1xminb	349	4	o~	o~	CD
cord-299281-5z1xminb	349	5	chain	chain	NN
cord-299281-5z1xminb	349	6	of	of	IN
cord-299281-5z1xminb	349	7	the	the	DT
cord-299281-5z1xminb	349	8	T	t	NN
cord-299281-5z1xminb	349	9	-	-	HYPH
cord-299281-5z1xminb	349	10	cell	cell	NN
cord-299281-5z1xminb	349	11	receptor	receptor	NN
cord-299281-5z1xminb	349	12	assembles	assemble	VBZ
cord-299281-5z1xminb	349	13	with	with	IN
cord-299281-5z1xminb	349	14	the	the	DT
cord-299281-5z1xminb	349	15	CD3	CD3	NNP
cord-299281-5z1xminb	349	16	6	6	CD
cord-299281-5z1xminb	349	17	chain	chain	NN
cord-299281-5z1xminb	349	18	via	via	IN
cord-299281-5z1xminb	349	19	interaction	interaction	NN
cord-299281-5z1xminb	349	20	between	between	IN
cord-299281-5z1xminb	349	21	charged	charge	VBN
cord-299281-5z1xminb	349	22	transmembrane	transmembrane	NN
cord-299281-5z1xminb	349	23	residues	residue	NNS
cord-299281-5z1xminb	349	24	(	(	-LRB-
cord-299281-5z1xminb	349	25	Manolios	Manolios	NNP
cord-299281-5z1xminb	349	26	et	et	FW
cord-299281-5z1xminb	349	27	al	al	NNP
cord-299281-5z1xminb	349	28	.	.	NNP
cord-299281-5z1xminb	349	29	,	,	,
cord-299281-5z1xminb	349	30	1990	1990	CD
cord-299281-5z1xminb	349	31	;	;	:
cord-299281-5z1xminb	349	32	Cosson	Cosson	NNP
cord-299281-5z1xminb	349	33	et	et	NNP
cord-299281-5z1xminb	349	34	al	al	NNP
cord-299281-5z1xminb	349	35	.	.	NNP
cord-299281-5z1xminb	349	36	,	,	,
cord-299281-5z1xminb	349	37	1991	1991	CD
cord-299281-5z1xminb	349	38	)	)	-RRB-
cord-299281-5z1xminb	349	39	.	.	.
cord-299281-5z1xminb	350	1	It	-PRON-	PRP
cord-299281-5z1xminb	350	2	is	be	VBZ
cord-299281-5z1xminb	350	3	not	not	RB
cord-299281-5z1xminb	350	4	clear	clear	JJ
cord-299281-5z1xminb	350	5	how	how	WRB
cord-299281-5z1xminb	350	6	the	the	DT
cord-299281-5z1xminb	350	7	transmembrane	transmembrane	NN
cord-299281-5z1xminb	350	8	domain	domain	NN
cord-299281-5z1xminb	350	9	of	of	IN
cord-299281-5z1xminb	350	10	Gml	Gml	NNP
cord-299281-5z1xminb	350	11	molecules	molecule	NNS
cord-299281-5z1xminb	350	12	might	may	MD
cord-299281-5z1xminb	350	13	interact	interact	VB
cord-299281-5z1xminb	350	14	to	to	TO
cord-299281-5z1xminb	350	15	form	form	VB
cord-299281-5z1xminb	350	16	a	a	DT
cord-299281-5z1xminb	350	17	large	large	JJ
cord-299281-5z1xminb	350	18	oligomer	oligomer	NN
cord-299281-5z1xminb	350	19	.	.	.
cord-299281-5z1xminb	351	1	The	the	DT
cord-299281-5z1xminb	351	2	large	large	JJ
cord-299281-5z1xminb	351	3	lumenal	lumenal	JJ
cord-299281-5z1xminb	351	4	domain	domain	NN
cord-299281-5z1xminb	351	5	of	of	IN
cord-299281-5z1xminb	351	6	Gml	Gml	NNP
cord-299281-5z1xminb	351	7	may	may	MD
cord-299281-5z1xminb	351	8	sterically	sterically	RB
cord-299281-5z1xminb	351	9	prevent	prevent	VB
cord-299281-5z1xminb	351	10	direct	direct	JJ
cord-299281-5z1xminb	351	11	interaction	interaction	NN
cord-299281-5z1xminb	351	12	between	between	IN
cord-299281-5z1xminb	351	13	many	many	JJ
cord-299281-5z1xminb	351	14	transmembrane	transmembrane	NN
cord-299281-5z1xminb	351	15	domains	domain	NNS
cord-299281-5z1xminb	351	16	to	to	TO
cord-299281-5z1xminb	351	17	form	form	VB
cord-299281-5z1xminb	351	18	an	an	DT
cord-299281-5z1xminb	351	19	oligomer	oligomer	NN
cord-299281-5z1xminb	351	20	.	.	.
cord-299281-5z1xminb	352	1	Furthermore	furthermore	RB
cord-299281-5z1xminb	352	2	,	,	,
cord-299281-5z1xminb	352	3	the	the	DT
cord-299281-5z1xminb	352	4	polar	polar	JJ
cord-299281-5z1xminb	352	5	face	face	NN
cord-299281-5z1xminb	352	6	of	of	IN
cord-299281-5z1xminb	352	7	the	the	DT
cord-299281-5z1xminb	352	8	ml	ml	NN
cord-299281-5z1xminb	352	9	domain	domain	NN
cord-299281-5z1xminb	352	10	itself	-PRON-	PRP
cord-299281-5z1xminb	352	11	could	could	MD
cord-299281-5z1xminb	352	12	be	be	VB
cord-299281-5z1xminb	352	13	expected	expect	VBN
cord-299281-5z1xminb	352	14	to	to	TO
cord-299281-5z1xminb	352	15	interact	interact	VB
cord-299281-5z1xminb	352	16	with	with	IN
cord-299281-5z1xminb	352	17	at	at	RB
cord-299281-5z1xminb	352	18	most	most	JJS
cord-299281-5z1xminb	352	19	one	one	CD
cord-299281-5z1xminb	352	20	or	or	CC
cord-299281-5z1xminb	352	21	two	two	CD
cord-299281-5z1xminb	352	22	similar	similar	JJ
cord-299281-5z1xminb	352	23	domains	domain	NNS
cord-299281-5z1xminb	352	24	.	.	.
cord-299281-5z1xminb	353	1	The	the	DT
cord-299281-5z1xminb	353	2	transmembrane	transmembrane	NN
cord-299281-5z1xminb	353	3	domains	domain	NNS
cord-299281-5z1xminb	353	4	of	of	IN
cord-299281-5z1xminb	353	5	these	these	DT
cord-299281-5z1xminb	353	6	proteins	protein	NNS
cord-299281-5z1xminb	353	7	might	may	MD
cord-299281-5z1xminb	353	8	interact	interact	VB
cord-299281-5z1xminb	353	9	to	to	TO
cord-299281-5z1xminb	353	10	form	form	VB
cord-299281-5z1xminb	353	11	small	small	JJ
cord-299281-5z1xminb	353	12	clusters	cluster	NNS
cord-299281-5z1xminb	353	13	(	(	-LRB-
cord-299281-5z1xminb	353	14	dimers	dimer	NNS
cord-299281-5z1xminb	353	15	or	or	CC
cord-299281-5z1xminb	353	16	trimers	trimer	NNS
cord-299281-5z1xminb	353	17	)	)	-RRB-
cord-299281-5z1xminb	353	18	of	of	IN
cord-299281-5z1xminb	353	19	Gml	Gml	NNP
cord-299281-5z1xminb	353	20	,	,	,
cord-299281-5z1xminb	353	21	mutant	mutant	JJ
cord-299281-5z1xminb	353	22	Gml	Gml	NNP
cord-299281-5z1xminb	353	23	proteins	protein	NNS
cord-299281-5z1xminb	353	24	,	,	,
cord-299281-5z1xminb	353	25	and	and	CC
cord-299281-5z1xminb	353	26	~mlG.	~mlg.	PDT
cord-299281-5z1xminb	353	27	These	these	DT
cord-299281-5z1xminb	353	28	clusters	cluster	NNS
cord-299281-5z1xminb	353	29	could	could	MD
cord-299281-5z1xminb	353	30	be	be	VB
cord-299281-5z1xminb	353	31	organized	organize	VBN
cord-299281-5z1xminb	353	32	into	into	IN
cord-299281-5z1xminb	353	33	large	large	JJ
cord-299281-5z1xminb	353	34	arrays	array	NNS
cord-299281-5z1xminb	353	35	by	by	IN
cord-299281-5z1xminb	353	36	interactions	interaction	NNS
cord-299281-5z1xminb	353	37	between	between	IN
cord-299281-5z1xminb	353	38	the	the	DT
cord-299281-5z1xminb	353	39	heads	head	NNS
cord-299281-5z1xminb	353	40	and/or	and/or	CC
cord-299281-5z1xminb	353	41	tails	tail	NNS
cord-299281-5z1xminb	353	42	of	of	IN
cord-299281-5z1xminb	353	43	proteins	protein	NNS
cord-299281-5z1xminb	353	44	in	in	IN
cord-299281-5z1xminb	353	45	different	different	JJ
cord-299281-5z1xminb	353	46	clusters	cluster	NNS
cord-299281-5z1xminb	353	47	.	.	.
cord-299281-5z1xminb	354	1	These	these	DT
cord-299281-5z1xminb	354	2	structures	structure	NNS
cord-299281-5z1xminb	354	3	might	may	MD
cord-299281-5z1xminb	354	4	contain	contain	VB
cord-299281-5z1xminb	354	5	lipids	lipid	NNS
cord-299281-5z1xminb	354	6	and	and	CC
cord-299281-5z1xminb	354	7	other	other	JJ
cord-299281-5z1xminb	354	8	endogenous	endogenous	JJ
cord-299281-5z1xminb	354	9	Golgi	Golgi	NNP
cord-299281-5z1xminb	354	10	-	-	HYPH
cord-299281-5z1xminb	354	11	resident	resident	NN
cord-299281-5z1xminb	354	12	proteins	protein	NNS
cord-299281-5z1xminb	354	13	.	.	.
cord-299281-5z1xminb	355	1	Association	Association	NNP
cord-299281-5z1xminb	355	2	into	into	IN
cord-299281-5z1xminb	355	3	such	such	PDT
cord-299281-5z1xminb	355	4	an	an	DT
cord-299281-5z1xminb	355	5	array	array	NN
cord-299281-5z1xminb	355	6	could	could	MD
cord-299281-5z1xminb	355	7	prevent	prevent	VB
cord-299281-5z1xminb	355	8	Golgi	Golgi	NNP
cord-299281-5z1xminb	355	9	-	-	HYPH
cord-299281-5z1xminb	355	10	resident	resident	NN
cord-299281-5z1xminb	355	11	proteins	protein	NNS
cord-299281-5z1xminb	355	12	from	from	IN
cord-299281-5z1xminb	355	13	entering	enter	VBG
cord-299281-5z1xminb	355	14	transport	transport	NN
cord-299281-5z1xminb	355	15	vesicles	vesicle	NNS
cord-299281-5z1xminb	355	16	and	and	CC
cord-299281-5z1xminb	355	17	thereby	thereby	RB
cord-299281-5z1xminb	355	18	function	function	VB
cord-299281-5z1xminb	355	19	as	as	IN
cord-299281-5z1xminb	355	20	a	a	DT
cord-299281-5z1xminb	355	21	mechanism	mechanism	NN
cord-299281-5z1xminb	355	22	for	for	IN
cord-299281-5z1xminb	355	23	their	-PRON-	PRP$
cord-299281-5z1xminb	355	24	retention	retention	NN
cord-299281-5z1xminb	355	25	.	.	.
cord-299281-5z1xminb	356	1	The	the	DT
cord-299281-5z1xminb	356	2	targeting	target	VBG
cord-299281-5z1xminb	356	3	signals	signal	NNS
cord-299281-5z1xminb	356	4	of	of	IN
cord-299281-5z1xminb	356	5	other	other	JJ
cord-299281-5z1xminb	356	6	Golgi	Golgi	NNP
cord-299281-5z1xminb	356	7	resident	resident	NN
cord-299281-5z1xminb	356	8	proteins	protein	NNS
cord-299281-5z1xminb	356	9	from	from	IN
cord-299281-5z1xminb	356	10	different	different	JJ
cord-299281-5z1xminb	356	11	Golgi	Golgi	NNP
cord-299281-5z1xminb	356	12	subcompartments	subcompartment	NNS
cord-299281-5z1xminb	356	13	also	also	RB
cord-299281-5z1xminb	356	14	reside	reside	VBP
cord-299281-5z1xminb	356	15	in	in	IN
cord-299281-5z1xminb	356	16	their	-PRON-	PRP$
cord-299281-5z1xminb	356	17	transmembrane	transmembrane	NN
cord-299281-5z1xminb	356	18	domains	domain	NNS
cord-299281-5z1xminb	356	19	(	(	-LRB-
cord-299281-5z1xminb	356	20	Aoki	Aoki	NNP
cord-299281-5z1xminb	356	21	et	et	NNP
cord-299281-5z1xminb	356	22	al	al	NNP
cord-299281-5z1xminb	356	23	.	.	NNP
cord-299281-5z1xminb	356	24	,	,	,
cord-299281-5z1xminb	356	25	1992	1992	CD
cord-299281-5z1xminb	356	26	;	;	:
cord-299281-5z1xminb	356	27	Burke	Burke	NNP
cord-299281-5z1xminb	356	28	et	et	NNP
cord-299281-5z1xminb	356	29	al	al	NNP
cord-299281-5z1xminb	356	30	.	.	.
cord-299281-5z1xminb	357	1	,	,	,
cord-299281-5z1xminb	357	2	1992	1992	CD
cord-299281-5z1xminb	357	3	;	;	:
cord-299281-5z1xminb	357	4	Colley	Colley	NNP
cord-299281-5z1xminb	357	5	et	et	NNP
cord-299281-5z1xminb	357	6	al	al	NNP
cord-299281-5z1xminb	357	7	.	.	.
cord-299281-5z1xminb	358	1	,	,	,
cord-299281-5z1xminb	358	2	1992	1992	CD
cord-299281-5z1xminb	358	3	;	;	:
cord-299281-5z1xminb	358	4	Munro	Munro	NNP
cord-299281-5z1xminb	358	5	,	,	,
cord-299281-5z1xminb	358	6	1991	1991	CD
cord-299281-5z1xminb	358	7	;	;	:
cord-299281-5z1xminb	358	8	Nilsson	Nilsson	NNP
cord-299281-5z1xminb	358	9	et	et	NNP
cord-299281-5z1xminb	358	10	al	al	NNP
cord-299281-5z1xminb	358	11	.	.	NNP
cord-299281-5z1xminb	358	12	,	,	,
cord-299281-5z1xminb	358	13	1991	1991	CD
cord-299281-5z1xminb	358	14	;	;	:
cord-299281-5z1xminb	359	1	Russo	Russo	NNP
cord-299281-5z1xminb	359	2	et	et	NNP
cord-299281-5z1xminb	359	3	al	al	NNP
cord-299281-5z1xminb	359	4	.	.	NNP
cord-299281-5z1xminb	359	5	,	,	,
cord-299281-5z1xminb	359	6	1992	1992	CD
cord-299281-5z1xminb	360	1	;	;	:
cord-299281-5z1xminb	360	2	Tang	Tang	NNP
cord-299281-5z1xminb	360	3	et	et	NNP
cord-299281-5z1xminb	360	4	al	al	NNP
cord-299281-5z1xminb	360	5	.	.	.
cord-299281-5z1xminb	361	1	,	,	,
cord-299281-5z1xminb	361	2	1992	1992	CD
cord-299281-5z1xminb	361	3	;	;	:
cord-299281-5z1xminb	361	4	Teasdale	Teasdale	NNP
cord-299281-5z1xminb	361	5	et	et	NNP
cord-299281-5z1xminb	361	6	al	al	NNP
cord-299281-5z1xminb	361	7	.	.	NNP
cord-299281-5z1xminb	361	8	,	,	,
cord-299281-5z1xminb	361	9	1992	1992	CD
cord-299281-5z1xminb	361	10	;	;	:
cord-299281-5z1xminb	361	11	Wo	will	MD
cord-299281-5z1xminb	361	12	nt	not	RB
cord-299281-5z1xminb	361	13	et	et	FW
cord-299281-5z1xminb	361	14	al	al	NNP
cord-299281-5z1xminb	361	15	.	.	NNP
cord-299281-5z1xminb	361	16	,	,	,
cord-299281-5z1xminb	361	17	1992	1992	CD
cord-299281-5z1xminb	361	18	)	)	-RRB-
cord-299281-5z1xminb	361	19	,	,	,
cord-299281-5z1xminb	361	20	although	although	IN
cord-299281-5z1xminb	361	21	flanking	flanking	JJ
cord-299281-5z1xminb	361	22	domains	domain	NNS
cord-299281-5z1xminb	361	23	also	also	RB
cord-299281-5z1xminb	361	24	contribute	contribute	VBP
cord-299281-5z1xminb	361	25	to	to	IN
cord-299281-5z1xminb	361	26	retention	retention	NN
cord-299281-5z1xminb	361	27	efficiency	efficiency	NN
cord-299281-5z1xminb	361	28	.	.	.
cord-299281-5z1xminb	362	1	No	no	DT
cord-299281-5z1xminb	362	2	obvious	obvious	JJ
cord-299281-5z1xminb	362	3	homologies	homology	NNS
cord-299281-5z1xminb	362	4	exist	exist	VBP
cord-299281-5z1xminb	362	5	between	between	IN
cord-299281-5z1xminb	362	6	the	the	DT
cord-299281-5z1xminb	362	7	primary	primary	JJ
cord-299281-5z1xminb	362	8	sequences	sequence	NNS
cord-299281-5z1xminb	362	9	of	of	IN
cord-299281-5z1xminb	362	10	these	these	DT
cord-299281-5z1xminb	362	11	membrane	membrane	NN
cord-299281-5z1xminb	362	12	-	-	HYPH
cord-299281-5z1xminb	362	13	spanning	span	VBG
cord-299281-5z1xminb	362	14	domains	domain	NNS
cord-299281-5z1xminb	362	15	,	,	,
cord-299281-5z1xminb	362	16	even	even	RB
cord-299281-5z1xminb	362	17	in	in	IN
cord-299281-5z1xminb	362	18	enzymes	enzyme	NNS
cord-299281-5z1xminb	362	19	thought	think	VBN
cord-299281-5z1xminb	362	20	to	to	TO
cord-299281-5z1xminb	362	21	be	be	VB
cord-299281-5z1xminb	362	22	enriched	enrich	VBN
cord-299281-5z1xminb	362	23	in	in	IN
cord-299281-5z1xminb	362	24	the	the	DT
cord-299281-5z1xminb	362	25	same	same	JJ
cord-299281-5z1xminb	362	26	subcompartment	subcompartment	NN
cord-299281-5z1xminb	362	27	.	.	.
cord-299281-5z1xminb	363	1	Thus	thus	RB
cord-299281-5z1xminb	363	2	,	,	,
cord-299281-5z1xminb	363	3	the	the	DT
cord-299281-5z1xminb	363	4	membrane	membrane	NN
cord-299281-5z1xminb	363	5	composition	composition	NN
cord-299281-5z1xminb	363	6	of	of	IN
cord-299281-5z1xminb	363	7	Golgi	Golgi	NNP
cord-299281-5z1xminb	363	8	subcompartments	subcompartment	NNS
cord-299281-5z1xminb	363	9	may	may	MD
cord-299281-5z1xminb	363	10	be	be	VB
cord-299281-5z1xminb	363	11	responsible	responsible	JJ
cord-299281-5z1xminb	363	12	for	for	IN
cord-299281-5z1xminb	363	13	specific	specific	JJ
cord-299281-5z1xminb	363	14	retention	retention	NN
cord-299281-5z1xminb	363	15	of	of	IN
cord-299281-5z1xminb	363	16	resident	resident	JJ
cord-299281-5z1xminb	363	17	proteins	protein	NNS
cord-299281-5z1xminb	363	18	throughout	throughout	IN
cord-299281-5z1xminb	363	19	this	this	DT
cord-299281-5z1xminb	363	20	organelle	organelle	NN
cord-299281-5z1xminb	363	21	.	.	.
cord-299281-5z1xminb	364	1	Consistent	consistent	JJ
cord-299281-5z1xminb	364	2	with	with	IN
cord-299281-5z1xminb	364	3	this	this	DT
cord-299281-5z1xminb	364	4	,	,	,
cord-299281-5z1xminb	364	5	no	no	DT
cord-299281-5z1xminb	364	6	soluble	soluble	JJ
cord-299281-5z1xminb	364	7	resident	resident	NN
cord-299281-5z1xminb	364	8	Golgi	Golgi	NNP
cord-299281-5z1xminb	364	9	proteins	protein	NNS
cord-299281-5z1xminb	364	10	have	have	VBP
cord-299281-5z1xminb	364	11	been	be	VBN
cord-299281-5z1xminb	364	12	detected	detect	VBN
cord-299281-5z1xminb	364	13	to	to	IN
cord-299281-5z1xminb	364	14	date	date	NN
cord-299281-5z1xminb	364	15	.	.	.
cord-299281-5z1xminb	365	1	Nilsson	Nilsson	NNP
cord-299281-5z1xminb	365	2	et	et	NNP
cord-299281-5z1xminb	365	3	al	al	NNP
cord-299281-5z1xminb	365	4	.	.	.
cord-299281-5z1xminb	366	1	(	(	-LRB-
cord-299281-5z1xminb	366	2	1993	1993	CD
cord-299281-5z1xminb	366	3	)	)	-RRB-
cord-299281-5z1xminb	366	4	have	have	VBP
cord-299281-5z1xminb	366	5	proposed	propose	VBN
cord-299281-5z1xminb	366	6	that	that	IN
cord-299281-5z1xminb	366	7	the	the	DT
cord-299281-5z1xminb	366	8	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	366	9	tails	tail	NNS
cord-299281-5z1xminb	366	10	of	of	IN
cord-299281-5z1xminb	366	11	resident	resident	JJ
cord-299281-5z1xminb	366	12	proteins	protein	NNS
cord-299281-5z1xminb	366	13	in	in	IN
cord-299281-5z1xminb	366	14	sequential	sequential	JJ
cord-299281-5z1xminb	366	15	Golgi	Golgi	NNP
cord-299281-5z1xminb	366	16	stacks	stack	NNS
cord-299281-5z1xminb	366	17	may	may	MD
cord-299281-5z1xminb	366	18	in	in	IN
cord-299281-5z1xminb	366	19	-	-	HYPH
cord-299281-5z1xminb	366	20	teract	teract	NN
cord-299281-5z1xminb	366	21	(	(	-LRB-
cord-299281-5z1xminb	366	22	perhaps	perhaps	RB
cord-299281-5z1xminb	366	23	via	via	IN
cord-299281-5z1xminb	366	24	a	a	DT
cord-299281-5z1xminb	366	25	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	366	26	linking	link	VBG
cord-299281-5z1xminb	366	27	protein	protein	NN
cord-299281-5z1xminb	366	28	)	)	-RRB-
cord-299281-5z1xminb	366	29	to	to	TO
cord-299281-5z1xminb	366	30	maintain	maintain	VB
cord-299281-5z1xminb	366	31	the	the	DT
cord-299281-5z1xminb	366	32	stacks	stack	NNS
cord-299281-5z1xminb	366	33	in	in	IN
cord-299281-5z1xminb	366	34	close	close	JJ
cord-299281-5z1xminb	366	35	apposition	apposition	NN
cord-299281-5z1xminb	366	36	.	.	.
cord-299281-5z1xminb	367	1	The	the	DT
cord-299281-5z1xminb	367	2	formation	formation	NN
cord-299281-5z1xminb	367	3	of	of	IN
cord-299281-5z1xminb	367	4	Gml	Gml	NNP
cord-299281-5z1xminb	367	5	oligomers	oligomer	NNS
cord-299281-5z1xminb	367	6	may	may	MD
cord-299281-5z1xminb	367	7	reflect	reflect	VB
cord-299281-5z1xminb	367	8	association	association	NN
cord-299281-5z1xminb	367	9	with	with	IN
cord-299281-5z1xminb	367	10	such	such	JJ
cord-299281-5z1xminb	367	11	endogenous	endogenous	JJ
cord-299281-5z1xminb	367	12	structures	structure	NNS
cord-299281-5z1xminb	367	13	.	.	.
cord-299281-5z1xminb	368	1	The	the	DT
cord-299281-5z1xminb	368	2	combination	combination	NN
cord-299281-5z1xminb	368	3	of	of	IN
cord-299281-5z1xminb	368	4	overexpression	overexpression	NN
cord-299281-5z1xminb	368	5	and	and	CC
cord-299281-5z1xminb	368	6	our	-PRON-	PRP$
cord-299281-5z1xminb	368	7	metabolic	metabolic	JJ
cord-299281-5z1xminb	368	8	radiolabeling	radiolabeling	NN
cord-299281-5z1xminb	368	9	conditions	condition	NNS
cord-299281-5z1xminb	368	10	would	would	MD
cord-299281-5z1xminb	368	11	not	not	RB
cord-299281-5z1xminb	368	12	be	be	VB
cord-299281-5z1xminb	368	13	expected	expect	VBN
cord-299281-5z1xminb	368	14	to	to	TO
cord-299281-5z1xminb	368	15	reveal	reveal	VB
cord-299281-5z1xminb	368	16	any	any	DT
cord-299281-5z1xminb	368	17	long	long	RB
cord-299281-5z1xminb	368	18	-	-	HYPH
cord-299281-5z1xminb	368	19	lived	live	VBN
cord-299281-5z1xminb	368	20	endogenous	endogenous	JJ
cord-299281-5z1xminb	368	21	Golgi	Golgi	NNP
cord-299281-5z1xminb	368	22	-	-	HYPH
cord-299281-5z1xminb	368	23	resident	resident	NN
cord-299281-5z1xminb	368	24	proteins	protein	NNS
cord-299281-5z1xminb	368	25	that	that	WDT
cord-299281-5z1xminb	368	26	also	also	RB
cord-299281-5z1xminb	368	27	reside	reside	VBP
cord-299281-5z1xminb	368	28	in	in	IN
cord-299281-5z1xminb	368	29	the	the	DT
cord-299281-5z1xminb	368	30	oligomer	oligomer	NN
cord-299281-5z1xminb	368	31	.	.	.
cord-299281-5z1xminb	369	1	However	however	RB
cord-299281-5z1xminb	369	2	,	,	,
cord-299281-5z1xminb	369	3	longer	long	RBR
cord-299281-5z1xminb	369	4	radiolabeling	radiolabele	VBG
cord-299281-5z1xminb	369	5	conditions	condition	NNS
cord-299281-5z1xminb	369	6	in	in	IN
cord-299281-5z1xminb	369	7	stable	stable	JJ
cord-299281-5z1xminb	369	8	cell	cell	NN
cord-299281-5z1xminb	369	9	lines	line	NNS
cord-299281-5z1xminb	369	10	expressing	express	VBG
cord-299281-5z1xminb	369	11	Gml	Gml	NNP
cord-299281-5z1xminb	369	12	may	may	MD
cord-299281-5z1xminb	369	13	reveal	reveal	VB
cord-299281-5z1xminb	369	14	other	other	JJ
cord-299281-5z1xminb	369	15	putative	putative	JJ
cord-299281-5z1xminb	369	16	components	component	NNS
cord-299281-5z1xminb	369	17	of	of	IN
cord-299281-5z1xminb	369	18	the	the	DT
cord-299281-5z1xminb	369	19	oligomer	oligomer	NNP
cord-299281-5z1xminb	369	20	.	.	.
cord-299281-5z1xminb	370	1	We	-PRON-	PRP
cord-299281-5z1xminb	370	2	are	be	VBP
cord-299281-5z1xminb	370	3	currently	currently	RB
cord-299281-5z1xminb	370	4	performing	perform	VBG
cord-299281-5z1xminb	370	5	such	such	JJ
cord-299281-5z1xminb	370	6	experiments	experiment	NNS
cord-299281-5z1xminb	370	7	.	.	.
cord-299281-5z1xminb	371	1	Golgi	Golgi	NNP
cord-299281-5z1xminb	371	2	retention	retention	NN
cord-299281-5z1xminb	371	3	of	of	IN
cord-299281-5z1xminb	371	4	a	a	DT
cord-299281-5z1xminb	371	5	trans	trans	JJ
cord-299281-5z1xminb	371	6	-	-	JJ
cord-299281-5z1xminb	371	7	Golgi	Golgi	NNP
cord-299281-5z1xminb	371	8	membrane	membrane	NN
cord-299281-5z1xminb	371	9	protein	protein	NN
cord-299281-5z1xminb	371	10	,	,	,
cord-299281-5z1xminb	371	11	galactosyltransferase	galactosyltransferase	NNP
cord-299281-5z1xminb	371	12	,	,	,
cord-299281-5z1xminb	371	13	requires	require	VBZ
cord-299281-5z1xminb	371	14	cysteine	cysteine	NN
cord-299281-5z1xminb	371	15	and	and	CC
cord-299281-5z1xminb	371	16	histidine	histidine	NN
cord-299281-5z1xminb	371	17	residues	residue	NNS
cord-299281-5z1xminb	371	18	within	within	IN
cord-299281-5z1xminb	371	19	the	the	DT
cord-299281-5z1xminb	371	20	membrane	membrane	NN
cord-299281-5z1xminb	371	21	-	-	HYPH
cord-299281-5z1xminb	371	22	anchoring	anchor	VBG
cord-299281-5z1xminb	371	23	region	region	NN
cord-299281-5z1xminb	371	24	A	a	DT
cord-299281-5z1xminb	371	25	film	film	NN
cord-299281-5z1xminb	371	26	detection	detection	NN
cord-299281-5z1xminb	371	27	method	method	NN
cord-299281-5z1xminb	371	28	for	for	IN
cord-299281-5z1xminb	371	29	tritinmlabeled	tritinmlabeled	JJ
cord-299281-5z1xminb	371	30	proteins	protein	NNS
cord-299281-5z1xminb	371	31	and	and	CC
cord-299281-5z1xminb	371	32	nucleic	nucleic	JJ
cord-299281-5z1xminb	371	33	acids	acid	NNS
cord-299281-5z1xminb	371	34	in	in	IN
cord-299281-5z1xminb	371	35	polyacrylamide	polyacrylamide	NN
cord-299281-5z1xminb	371	36	gels	gel	NNS
cord-299281-5z1xminb	371	37	Synthetic	synthetic	JJ
cord-299281-5z1xminb	371	38	peptides	peptide	NNS
cord-299281-5z1xminb	371	39	mimic	mimic	VBP
cord-299281-5z1xminb	371	40	the	the	DT
cord-299281-5z1xminb	371	41	assembly	assembly	NN
cord-299281-5z1xminb	371	42	of	of	IN
cord-299281-5z1xminb	371	43	transmembrane	transmembrane	NNP
cord-299281-5z1xminb	371	44	glycoproteins	glycoprotein	NNS
cord-299281-5z1xminb	372	1	Intramembrane	Intramembrane	NNP
cord-299281-5z1xminb	372	2	helix	helix	NN
cord-299281-5z1xminb	372	3	-	-	HYPH
cord-299281-5z1xminb	372	4	helix	helix	NNP
cord-299281-5z1xminb	372	5	association	association	NNP
cord-299281-5z1xminb	372	6	in	in	IN
cord-299281-5z1xminb	372	7	oligomarization	oligomarization	NN
cord-299281-5z1xminb	372	8	and	and	CC
cord-299281-5z1xminb	372	9	transmembrane	transmembrane	NN
cord-299281-5z1xminb	372	10	signaling	signal	VBG
cord-299281-5z1xminb	372	11	The	the	DT
cord-299281-5z1xminb	372	12	transmambrane	transmambrane	NN
cord-299281-5z1xminb	372	13	and	and	CC
cord-299281-5z1xminb	372	14	flanking	flanking	JJ
cord-299281-5z1xminb	372	15	sequences	sequence	NNS
cord-299281-5z1xminb	372	16	of	of	IN
cord-299281-5z1xminb	372	17	81,2-N	81,2-n	CD
cord-299281-5z1xminb	373	1	-Acetylglucosaminyltransferase	-Acetylglucosaminyltransferase	NFP
cord-299281-5z1xminb	374	1	I	-PRON-	PRP
cord-299281-5z1xminb	374	2	specify	specify	VBP
cord-299281-5z1xminb	374	3	med	med	NNP
cord-299281-5z1xminb	374	4	/	/	SYM
cord-299281-5z1xminb	374	5	a/-Golgi	a/-Golgi	NNP
cord-299281-5z1xminb	374	6	localization	localization	NN
cord-299281-5z1xminb	375	1	The	the	DT
cord-299281-5z1xminb	375	2	signal	signal	NN
cord-299281-5z1xminb	375	3	anchor	anchor	NN
cord-299281-5z1xminb	375	4	and	and	CC
cord-299281-5z1xminb	375	5	stem	stem	NN
cord-299281-5z1xminb	375	6	regions	region	NNS
cord-299281-5z1xminb	375	7	of	of	IN
cord-299281-5z1xminb	375	8	the	the	DT
cord-299281-5z1xminb	375	9	~-galactoside	~-galactoside	NN
cord-299281-5z1xminb	375	10	a2,6-sialyltransferase	a2,6-sialyltransferase	.
cord-299281-5z1xminb	375	11	may	may	MD
cord-299281-5z1xminb	375	12	each	each	DT
cord-299281-5z1xminb	375	13	act	act	NN
cord-299281-5z1xminb	375	14	to	to	TO
cord-299281-5z1xminb	375	15	localize	localize	VB
cord-299281-5z1xminb	375	16	the	the	DT
cord-299281-5z1xminb	375	17	enzyme	enzyme	NN
cord-299281-5z1xminb	375	18	to	to	IN
cord-299281-5z1xminb	375	19	the	the	DT
cord-299281-5z1xminb	375	20	Golgi	Golgi	NNP
cord-299281-5z1xminb	375	21	apparatus	apparatus	NN
cord-299281-5z1xminb	375	22	Role	role	NN
cord-299281-5z1xminb	375	23	of	of	IN
cord-299281-5z1xminb	375	24	transmembrane	transmembrane	NN
cord-299281-5z1xminb	375	25	domain	domain	NN
cord-299281-5z1xminb	375	26	interactions	interaction	NNS
cord-299281-5z1xminb	375	27	in	in	IN
cord-299281-5z1xminb	375	28	the	the	DT
cord-299281-5z1xminb	375	29	assembly	assembly	NN
cord-299281-5z1xminb	375	30	of	of	IN
cord-299281-5z1xminb	375	31	class	class	NN
cord-299281-5z1xminb	375	32	H	h	NN
cord-299281-5z1xminb	375	33	MHC	MHC	NNP
cord-299281-5z1xminb	376	1	molecules	molecule	NNS
cord-299281-5z1xminb	376	2	Membrane	Membrane	NNP
cord-299281-5z1xminb	376	3	protein	protein	NNP
cord-299281-5z1xminb	376	4	association	association	NN
cord-299281-5z1xminb	376	5	by	by	IN
cord-299281-5z1xminb	376	6	potential	potential	JJ
cord-299281-5z1xminb	376	7	intramembrane	intramembrane	NNP
cord-299281-5z1xminb	376	8	charge	charge	NN
cord-299281-5z1xminb	376	9	pairs	pair	NNS
cord-299281-5z1xminb	376	10	Role	role	NN
cord-299281-5z1xminb	376	11	for	for	IN
cord-299281-5z1xminb	376	12	adenosine	adenosine	NN
cord-299281-5z1xminb	376	13	triphosphate	triphosphate	NN
cord-299281-5z1xminb	376	14	in	in	IN
cord-299281-5z1xminb	376	15	regulating	regulate	VBG
cord-299281-5z1xminb	376	16	the	the	DT
cord-299281-5z1xminb	376	17	assembly	assembly	NN
cord-299281-5z1xminb	376	18	and	and	CC
cord-299281-5z1xminb	376	19	transport	transport	NN
cord-299281-5z1xminb	376	20	of	of	IN
cord-299281-5z1xminb	376	21	vesicular	vesicular	JJ
cord-299281-5z1xminb	376	22	stomatitis	stomatitis	NN
cord-299281-5z1xminb	376	23	virus	virus	NN
cord-299281-5z1xminb	376	24	G	g	NN
cord-299281-5z1xminb	376	25	protein	protein	NN
cord-299281-5z1xminb	376	26	trimers	trimer	VBZ
cord-299281-5z1xminb	376	27	Differential	differential	JJ
cord-299281-5z1xminb	376	28	effects	effect	NNS
cord-299281-5z1xminb	376	29	of	of	IN
cord-299281-5z1xminb	376	30	mutations	mutation	NNS
cord-299281-5z1xminb	376	31	in	in	IN
cord-299281-5z1xminb	376	32	three	three	CD
cord-299281-5z1xminb	376	33	domains	domain	NNS
cord-299281-5z1xminb	376	34	on	on	IN
cord-299281-5z1xminb	376	35	folding	fold	VBG
cord-299281-5z1xminb	376	36	,	,	,
cord-299281-5z1xminb	376	37	quaternary	quaternary	JJ
cord-299281-5z1xminb	376	38	structure	structure	NN
cord-299281-5z1xminb	376	39	,	,	,
cord-299281-5z1xminb	376	40	and	and	CC
cord-299281-5z1xminb	376	41	intraoellular	intraoellular	JJ
cord-299281-5z1xminb	376	42	transport	transport	NN
cord-299281-5z1xminb	376	43	of	of	IN
cord-299281-5z1xminb	376	44	vesicular	vesicular	JJ
cord-299281-5z1xminb	376	45	stomatitis	stomatitis	NN
cord-299281-5z1xminb	376	46	virus	virus	NN
cord-299281-5z1xminb	376	47	G	g	NN
cord-299281-5z1xminb	376	48	protein	protein	NN
cord-299281-5z1xminb	376	49	Folding	folding	NN
cord-299281-5z1xminb	376	50	and	and	CC
cord-299281-5z1xminb	376	51	assembly	assembly	NN
cord-299281-5z1xminb	376	52	of	of	IN
cord-299281-5z1xminb	376	53	viral	viral	JJ
cord-299281-5z1xminb	376	54	membrane	membrane	NN
cord-299281-5z1xminb	376	55	proteins	protein	NNS
cord-299281-5z1xminb	377	1	Eukaryotic	eukaryotic	JJ
cord-299281-5z1xminb	377	2	transient	transient	JJ
cord-299281-5z1xminb	377	3	expression	expression	NN
cord-299281-5z1xminb	377	4	system	system	NN
cord-299281-5z1xminb	377	5	based	base	VBN
cord-299281-5z1xminb	377	6	on	on	IN
cord-299281-5z1xminb	377	7	recombinant	recombinant	JJ
cord-299281-5z1xminb	377	8	vaccinia	vaccinia	NN
cord-299281-5z1xminb	377	9	virus	virus	NN
cord-299281-5z1xminb	377	10	that	that	WDT
cord-299281-5z1xminb	377	11	synthesizes	synthesize	VBZ
cord-299281-5z1xminb	377	12	bacteriophage	bacteriophage	NN
cord-299281-5z1xminb	377	13	T7	T7	NNP
cord-299281-5z1xminb	377	14	RNA	RNA	NNP
cord-299281-5z1xminb	377	15	polymerase	polymerase	NN
cord-299281-5z1xminb	377	16	Subunit	Subunit	NNP
cord-299281-5z1xminb	377	17	structure	structure	NN
cord-299281-5z1xminb	377	18	of	of	IN
cord-299281-5z1xminb	377	19	human	human	JJ
cord-299281-5z1xminb	377	20	erythrocyte	erythrocyte	NN
cord-299281-5z1xminb	377	21	glycophorin	glycophorin	XX
cord-299281-5z1xminb	377	22	A	a	DT
cord-299281-5z1xminb	377	23	Cell	cell	NN
cord-299281-5z1xminb	377	24	biology	biology	NN
cord-299281-5z1xminb	377	25	of	of	IN
cord-299281-5z1xminb	377	26	viruses	virus	NNS
cord-299281-5z1xminb	377	27	that	that	WDT
cord-299281-5z1xminb	377	28	assemble	assemble	VBP
cord-299281-5z1xminb	377	29	along	along	IN
cord-299281-5z1xminb	377	30	the	the	DT
cord-299281-5z1xminb	377	31	biosynthetic	biosynthetic	JJ
cord-299281-5z1xminb	377	32	pathway	pathway	NN
cord-299281-5z1xminb	377	33	Cell	cell	NN
cord-299281-5z1xminb	377	34	-	-	HYPH
cord-299281-5z1xminb	377	35	surface	surface	NN
cord-299281-5z1xminb	377	36	expression	expression	NN
cord-299281-5z1xminb	377	37	of	of	IN
cord-299281-5z1xminb	377	38	a	a	DT
cord-299281-5z1xminb	377	39	membrane	membrane	NN
cord-299281-5z1xminb	377	40	-	-	HYPH
cord-299281-5z1xminb	377	41	anchored	anchor	VBN
cord-299281-5z1xminb	377	42	form	form	NN
cord-299281-5z1xminb	377	43	of	of	IN
cord-299281-5z1xminb	377	44	the	the	DT
cord-299281-5z1xminb	377	45	human	human	JJ
cord-299281-5z1xminb	377	46	chorionic	chorionic	NN
cord-299281-5z1xminb	377	47	gonadotropin	gonadotropin	NN
cord-299281-5z1xminb	377	48	a	a	DT
cord-299281-5z1xminb	377	49	subunit	subunit	NN
cord-299281-5z1xminb	377	50	Effects	effect	NNS
cord-299281-5z1xminb	377	51	of	of	IN
cord-299281-5z1xminb	377	52	altered	alter	VBN
cord-299281-5z1xminb	377	53	cy~-plasmic	cy~-plasmic	NNP
cord-299281-5z1xminb	377	54	domains	domain	NNS
cord-299281-5z1xminb	377	55	on	on	IN
cord-299281-5z1xminb	377	56	transport	transport	NN
cord-299281-5z1xminb	377	57	of	of	IN
cord-299281-5z1xminb	377	58	the	the	DT
cord-299281-5z1xminb	377	59	vesicular	vesicular	JJ
cord-299281-5z1xminb	377	60	stomatitis	stomatitis	NN
cord-299281-5z1xminb	377	61	virus	virus	NN
cord-299281-5z1xminb	377	62	giycoprotein	giycoprotein	NNP
cord-299281-5z1xminb	377	63	are	be	VBP
cord-299281-5z1xminb	377	64	transferable	transferable	JJ
cord-299281-5z1xminb	377	65	to	to	IN
cord-299281-5z1xminb	377	66	other	other	JJ
cord-299281-5z1xminb	377	67	proteins	protein	NNS
cord-299281-5z1xminb	377	68	Rapid	rapid	JJ
cord-299281-5z1xminb	377	69	and	and	CC
cord-299281-5z1xminb	377	70	efficient	efficient	JJ
cord-299281-5z1xminb	377	71	sitespecific	sitespecific	NN
cord-299281-5z1xminb	377	72	mutagenesis	mutagenesis	NN
cord-299281-5z1xminb	377	73	without	without	IN
cord-299281-5z1xminb	377	74	phenotypic	phenotypic	NN
cord-299281-5z1xminb	377	75	selection	selection	NN
cord-299281-5z1xminb	377	76	Cleavage	cleavage	NN
cord-299281-5z1xminb	377	77	of	of	IN
cord-299281-5z1xminb	377	78	structural	structural	JJ
cord-299281-5z1xminb	377	79	proteins	protein	NNS
cord-299281-5z1xminb	377	80	during	during	IN
cord-299281-5z1xminb	377	81	the	the	DT
cord-299281-5z1xminb	377	82	assembly	assembly	NN
cord-299281-5z1xminb	377	83	of	of	IN
cord-299281-5z1xminb	377	84	the	the	DT
cord-299281-5z1xminb	377	85	head	head	NN
cord-299281-5z1xminb	377	86	of	of	IN
cord-299281-5z1xminb	377	87	bacteriophage	bacteriophage	NN
cord-299281-5z1xminb	377	88	T4	T4	NNP
cord-299281-5z1xminb	378	1	The	the	DT
cord-299281-5z1xminb	378	2	interaction	interaction	NN
cord-299281-5z1xminb	378	3	of	of	IN
cord-299281-5z1xminb	378	4	antibody	antibody	NN
cord-299281-5z1xminb	378	5	with	with	IN
cord-299281-5z1xminb	378	6	the	the	DT
cord-299281-5z1xminb	378	7	major	major	JJ
cord-299281-5z1xminb	378	8	surface	surface	NN
cord-299281-5z1xminb	378	9	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	378	10	of	of	IN
cord-299281-5z1xminb	378	11	vesicular	vesicular	JJ
cord-299281-5z1xminb	378	12	stomatitis	stomatitis	NN
cord-299281-5z1xminb	378	13	virus	virus	NN
cord-299281-5z1xminb	379	1	Glycophorin	Glycophorin	NNP
cord-299281-5z1xminb	379	2	A	a	DT
cord-299281-5z1xminb	379	3	dimerization	dimerization	NN
cord-299281-5z1xminb	379	4	is	be	VBZ
cord-299281-5z1xminb	379	5	driven	drive	VBN
cord-299281-5z1xminb	379	6	by	by	IN
cord-299281-5z1xminb	379	7	specific	specific	JJ
cord-299281-5z1xminb	379	8	interactions	interaction	NNS
cord-299281-5z1xminb	379	9	between	between	IN
cord-299281-5z1xminb	379	10	transmembrane	transmembrane	ADD
cord-299281-5z1xminb	379	11	a	a	LS
cord-299281-5z1xminb	379	12	-	-	:
cord-299281-5z1xminb	379	13	helices	helix	NNS
cord-299281-5z1xminb	379	14	Polarized	polarized	JJ
cord-299281-5z1xminb	379	15	apical	apical	JJ
cord-299281-5z1xminb	379	16	distribution	distribution	NN
cord-299281-5z1xminb	379	17	of	of	IN
cord-299281-5z1xminb	379	18	glycosyl	glycosyl	JJ
cord-299281-5z1xminb	379	19	-	-	HYPH
cord-299281-5z1xminb	379	20	phosphatidylinositolanchored	phosphatidylinositolanchore	VBN
cord-299281-5z1xminb	379	21	proteins	protein	NNS
cord-299281-5z1xminb	379	22	in	in	IN
cord-299281-5z1xminb	379	23	a	a	DT
cord-299281-5z1xminb	379	24	renal	renal	JJ
cord-299281-5z1xminb	379	25	epithelial	epithelial	JJ
cord-299281-5z1xminb	379	26	cell	cell	NN
cord-299281-5z1xminb	379	27	line	line	NN
cord-299281-5z1xminb	379	28	Golgi	Golgi	NNP
cord-299281-5z1xminb	379	29	retention	retention	NN
cord-299281-5z1xminb	379	30	signals	signal	NNS
cord-299281-5z1xminb	379	31	:	:	:
cord-299281-5z1xminb	379	32	do	do	VBP
cord-299281-5z1xminb	379	33	membranes	membrane	NNS
cord-299281-5z1xminb	379	34	hold	hold	VB
cord-299281-5z1xminb	379	35	the	the	DT
cord-299281-5z1xminb	379	36	key	key	NN
cord-299281-5z1xminb	379	37	?	?	.
cord-299281-5z1xminb	380	1	A	a	DT
cord-299281-5z1xminb	380	2	specific	specific	JJ
cord-299281-5z1xminb	380	3	transmembrane	transmembrane	NN
cord-299281-5z1xminb	380	4	domain	domain	NN
cord-299281-5z1xminb	380	5	of	of	IN
cord-299281-5z1xminb	380	6	a	a	DT
cord-299281-5z1xminb	380	7	coronavirus	coronavirus	NN
cord-299281-5z1xminb	380	8	E1	e1	NN
cord-299281-5z1xminb	380	9	giycoprotein	giycoprotein	NNP
cord-299281-5z1xminb	380	10	is	be	VBZ
cord-299281-5z1xminb	380	11	required	require	VBN
cord-299281-5z1xminb	380	12	for	for	IN
cord-299281-5z1xminb	380	13	its	-PRON-	PRP$
cord-299281-5z1xminb	380	14	retention	retention	NN
cord-299281-5z1xminb	380	15	in	in	IN
cord-299281-5z1xminb	380	16	the	the	DT
cord-299281-5z1xminb	380	17	Golgi	Golgi	NNP
cord-299281-5z1xminb	380	18	region	region	NN
cord-299281-5z1xminb	380	19	Influence	influence	NN
cord-299281-5z1xminb	380	20	of	of	IN
cord-299281-5z1xminb	380	21	new	new	JJ
cord-299281-5z1xminb	380	22	glycosylation	glycosylation	NN
cord-299281-5z1xminb	380	23	sites	site	NNS
cord-299281-5z1xminb	380	24	on	on	IN
cord-299281-5z1xminb	380	25	expression	expression	NN
cord-299281-5z1xminb	380	26	of	of	IN
cord-299281-5z1xminb	380	27	the	the	DT
cord-299281-5z1xminb	380	28	vesicular	vesicular	JJ
cord-299281-5z1xminb	380	29	stomatitis	stomatitis	NN
cord-299281-5z1xminb	380	30	virus	virus	NN
cord-299281-5z1xminb	380	31	G	g	NN
cord-299281-5z1xminb	380	32	protein	protein	NN
cord-299281-5z1xminb	380	33	at	at	IN
cord-299281-5z1xminb	380	34	the	the	DT
cord-299281-5z1xminb	380	35	plasma	plasma	NN
cord-299281-5z1xminb	380	36	membrane	membrane	NN
cord-299281-5z1xminb	381	1	A	a	DT
cord-299281-5z1xminb	381	2	single	single	JJ
cord-299281-5z1xminb	381	3	N	n	NN
cord-299281-5z1xminb	381	4	-	-	HYPH
cord-299281-5z1xminb	381	5	linked	link	VBN
cord-299281-5z1xminb	381	6	ollgosaccharide	ollgosaccharide	NN
cord-299281-5z1xminb	381	7	at	at	IN
cord-299281-5z1xminb	381	8	either	either	DT
cord-299281-5z1xminb	381	9	of	of	IN
cord-299281-5z1xminb	381	10	the	the	DT
cord-299281-5z1xminb	381	11	two	two	CD
cord-299281-5z1xminb	381	12	normal	normal	JJ
cord-299281-5z1xminb	381	13	sites	site	NNS
cord-299281-5z1xminb	381	14	is	be	VBZ
cord-299281-5z1xminb	381	15	sufficient	sufficient	JJ
cord-299281-5z1xminb	381	16	for	for	IN
cord-299281-5z1xminb	381	17	transport	transport	NN
cord-299281-5z1xminb	381	18	of	of	IN
cord-299281-5z1xminb	381	19	vesicular	vesicular	JJ
cord-299281-5z1xminb	381	20	stomatitis	stomatitis	NN
cord-299281-5z1xminb	381	21	virus	virus	NN
cord-299281-5z1xminb	381	22	G	g	NN
cord-299281-5z1xminb	381	23	protein	protein	NN
cord-299281-5z1xminb	381	24	to	to	IN
cord-299281-5z1xminb	381	25	the	the	DT
cord-299281-5z1xminb	381	26	cell	cell	NN
cord-299281-5z1xminb	381	27	surface	surface	NN
cord-299281-5z1xminb	382	1	The	the	DT
cord-299281-5z1xminb	382	2	E1	e1	NN
cord-299281-5z1xminb	382	3	glycoprotein	glycoprotein	NN
cord-299281-5z1xminb	382	4	of	of	IN
cord-299281-5z1xminb	382	5	an	an	DT
cord-299281-5z1xminb	382	6	avian	avian	JJ
cord-299281-5z1xminb	382	7	corouavirus	corouavirus	NN
cord-299281-5z1xminb	382	8	is	be	VBZ
cord-299281-5z1xminb	382	9	targeted	target	VBN
cord-299281-5z1xminb	382	10	to	to	IN
cord-299281-5z1xminb	382	11	the	the	DT
cord-299281-5z1xminb	382	12	cis	cis	NNP
cord-299281-5z1xminb	382	13	Golgi	Golgi	NNP
cord-299281-5z1xminb	382	14	complex	complex	NNP
cord-299281-5z1xminb	382	15	Retention	Retention	NNP
cord-299281-5z1xminb	382	16	of	of	IN
cord-299281-5z1xminb	382	17	a	a	DT
cord-299281-5z1xminb	382	18	cis	cis	NNP
cord-299281-5z1xminb	382	19	Golgi	Golgi	NNP
cord-299281-5z1xminb	382	20	protein	protein	NN
cord-299281-5z1xminb	382	21	requires	require	VBZ
cord-299281-5z1xminb	382	22	polar	polar	JJ
cord-299281-5z1xminb	382	23	residues	residue	NNS
cord-299281-5z1xminb	382	24	on	on	IN
cord-299281-5z1xminb	382	25	one	one	CD
cord-299281-5z1xminb	382	26	face	face	NN
cord-299281-5z1xminb	382	27	of	of	IN
cord-299281-5z1xminb	382	28	a	a	DT
cord-299281-5z1xminb	382	29	predicted	predict	VBN
cord-299281-5z1xminb	382	30	a	a	DT
cord-299281-5z1xminb	382	31	-	-	HYPH
cord-299281-5z1xminb	382	32	helix	helix	NN
cord-299281-5z1xminb	382	33	in	in	IN
cord-299281-5z1xminb	382	34	the	the	DT
cord-299281-5z1xminb	382	35	transmembrane	transmembrane	NN
cord-299281-5z1xminb	382	36	domain	domain	NN
cord-299281-5z1xminb	382	37	Transmembrane	Transmembrane	NNP
cord-299281-5z1xminb	382	38	helical	helical	JJ
cord-299281-5z1xminb	382	39	interactions	interaction	NNS
cord-299281-5z1xminb	382	40	and	and	CC
cord-299281-5z1xminb	382	41	the	the	DT
cord-299281-5z1xminb	382	42	assembly	assembly	NN
cord-299281-5z1xminb	382	43	of	of	IN
cord-299281-5z1xminb	382	44	the	the	DT
cord-299281-5z1xminb	382	45	T	T	NNP
cord-299281-5z1xminb	382	46	cell	cell	NN
cord-299281-5z1xminb	382	47	receptor	receptor	NN
cord-299281-5z1xminb	382	48	complex	complex	NN
cord-299281-5z1xminb	382	49	Sequences	Sequences	NNPS
cord-299281-5z1xminb	382	50	within	within	IN
cord-299281-5z1xminb	382	51	and	and	CC
cord-299281-5z1xminb	382	52	adjacent	adjacent	JJ
cord-299281-5z1xminb	382	53	to	to	IN
cord-299281-5z1xminb	382	54	the	the	DT
cord-299281-5z1xminb	382	55	transmembrane	transmembrane	NN
cord-299281-5z1xminb	382	56	segment	segment	NN
cord-299281-5z1xminb	382	57	of	of	IN
cord-299281-5z1xminb	382	58	a-2,6-sialyltransferase	a-2,6-sialyltransferase	NNP
cord-299281-5z1xminb	382	59	specify	specify	VB
cord-299281-5z1xminb	382	60	Golgi	Golgi	NNP
cord-299281-5z1xminb	382	61	retention	retention	NN
cord-299281-5z1xminb	382	62	.	.	.
cord-299281-5z1xminb	383	1	EMBO	EMBO	NNP
cord-299281-5z1xminb	384	1	(	(	-LRB-
cord-299281-5z1xminb	384	2	Eur	Eur	NNP
cord-299281-5z1xminb	385	1	The	the	DT
cord-299281-5z1xminb	385	2	membrane	membrane	NN
cord-299281-5z1xminb	385	3	spanning	span	VBG
cord-299281-5z1xminb	385	4	domain	domain	NN
cord-299281-5z1xminb	385	5	of	of	IN
cord-299281-5z1xminb	385	6	8	8	CD
cord-299281-5z1xminb	385	7	-	-	SYM
cord-299281-5z1xminb	385	8	1,4-galactosyltransferase	1,4-galactosyltransferase	CD
cord-299281-5z1xminb	385	9	specifies	specify	VBZ
cord-299281-5z1xminb	385	10	trans	trans	NNP
cord-299281-5z1xminb	385	11	Golgi	Golgi	NNP
cord-299281-5z1xminb	385	12	localization	localization	NN
cord-299281-5z1xminb	385	13	Overlapping	overlap	VBG
cord-299281-5z1xminb	385	14	distribution	distribution	NN
cord-299281-5z1xminb	385	15	of	of	IN
cord-299281-5z1xminb	385	16	two	two	CD
cord-299281-5z1xminb	385	17	glycosyltransferases	glycosyltransferase	NNS
cord-299281-5z1xminb	385	18	in	in	IN
cord-299281-5z1xminb	385	19	the	the	DT
cord-299281-5z1xminb	385	20	Golgi	Golgi	NNP
cord-299281-5z1xminb	385	21	apparatus	apparatus	NN
cord-299281-5z1xminb	385	22	of	of	IN
cord-299281-5z1xminb	385	23	HeLa	HeLa	NNP
cord-299281-5z1xminb	385	24	cells	cell	VBZ
cord-299281-5z1xminb	385	25	A	a	DT
cord-299281-5z1xminb	385	26	new	new	JJ
cord-299281-5z1xminb	385	27	cationic	cationic	JJ
cord-299281-5z1xminb	385	28	liposome	liposome	NN
cord-299281-5z1xminb	385	29	reagent	reagent	NN
cord-299281-5z1xminb	385	30	mediating	mediate	VBG
cord-299281-5z1xminb	385	31	nearly	nearly	RB
cord-299281-5z1xminb	385	32	quantitative	quantitative	JJ
cord-299281-5z1xminb	385	33	transfeodon	transfeodon	NN
cord-299281-5z1xminb	385	34	of	of	IN
cord-299281-5z1xminb	385	35	animal	animal	NN
cord-299281-5z1xminb	385	36	cells	cell	NNS
cord-299281-5z1xminb	386	1	4-galactosyltransferase	4-galactosyltransferase	CD
cord-299281-5z1xminb	386	2	:	:	:
cord-299281-5z1xminb	386	3	a	a	DT
cord-299281-5z1xminb	386	4	short	short	JJ
cord-299281-5z1xminb	386	5	NH2-terminal	nh2-terminal	NN
cord-299281-5z1xminb	386	6	fragment	fragment	NN
cord-299281-5z1xminb	386	7	that	that	WDT
cord-299281-5z1xminb	386	8	includes	include	VBZ
cord-299281-5z1xminb	386	9	the	the	DT
cord-299281-5z1xminb	386	10	cytoplasmic	cytoplasmic	JJ
cord-299281-5z1xminb	386	11	and	and	CC
cord-299281-5z1xminb	386	12	transmembrane	transmembrane	NN
cord-299281-5z1xminb	386	13	domain	domain	NN
cord-299281-5z1xminb	386	14	is	be	VBZ
cord-299281-5z1xminb	386	15	sufficient	sufficient	JJ
cord-299281-5z1xminb	386	16	for	for	IN
cord-299281-5z1xminb	386	17	Golgi	Golgi	NNP
cord-299281-5z1xminb	386	18	retention	retention	NN
cord-299281-5z1xminb	386	19	Ire	ire	NN
cord-299281-5z1xminb	386	20	-	-	,
cord-299281-5z1xminb	386	21	and	and	CC
cord-299281-5z1xminb	386	22	post	post	JJ
cord-299281-5z1xminb	386	23	-	-	JJ
cord-299281-5z1xminb	386	24	Golgi	golgi	JJ
cord-299281-5z1xminb	386	25	vacuoles	vacuole	NNS
cord-299281-5z1xminb	386	26	operate	operate	VBP
cord-299281-5z1xminb	386	27	in	in	IN
cord-299281-5z1xminb	386	28	the	the	DT
cord-299281-5z1xminb	386	29	transport	transport	NN
cord-299281-5z1xminb	386	30	of	of	IN
cord-299281-5z1xminb	386	31	Semliki	Semliki	NNP
cord-299281-5z1xminb	386	32	Forest	Forest	NNP
cord-299281-5z1xminb	386	33	virus	virus	NN
cord-299281-5z1xminb	386	34	membrane	membrane	NN
cord-299281-5z1xminb	386	35	glycoproteins	glycoprotein	NNS
cord-299281-5z1xminb	386	36	to	to	IN
cord-299281-5z1xminb	386	37	the	the	DT
cord-299281-5z1xminb	386	38	cell	cell	NN
cord-299281-5z1xminb	386	39	surface	surface	NN
cord-299281-5z1xminb	386	40	A	a	DT
cord-299281-5z1xminb	386	41	sequence	sequence	NN
cord-299281-5z1xminb	386	42	in	in	IN
cord-299281-5z1xminb	386	43	the	the	DT
cord-299281-5z1xminb	386	44	transmembrane	transmembrane	NN
cord-299281-5z1xminb	386	45	region	region	NN
cord-299281-5z1xminb	386	46	of	of	IN
cord-299281-5z1xminb	386	47	growth	growth	NN
cord-299281-5z1xminb	386	48	factor	factor	NN
cord-299281-5z1xminb	386	49	receptors	receptor	NNS
cord-299281-5z1xminb	386	50	with	with	IN
cord-299281-5z1xminb	386	51	tyrosine	tyrosine	NN
cord-299281-5z1xminb	386	52	kinase	kinase	NN
cord-299281-5z1xminb	386	53	activity	activity	NN
cord-299281-5z1xminb	387	1	mediates	mediate	VBZ
cord-299281-5z1xminb	387	2	dimerization	dimerization	VBG
cord-299281-5z1xminb	387	3	A	a	DT
cord-299281-5z1xminb	387	4	Golgi	Golgi	NNP
cord-299281-5z1xminb	387	5	retention	retention	NN
cord-299281-5z1xminb	387	6	signal	signal	NN
cord-299281-5z1xminb	387	7	in	in	IN
cord-299281-5z1xminb	387	8	a	a	DT
cord-299281-5z1xminb	387	9	membrane	membrane	NN
cord-299281-5z1xminb	387	10	-	-	HYPH
cord-299281-5z1xminb	387	11	spanning	span	VBG
cord-299281-5z1xminb	387	12	domain	domain	NN
cord-299281-5z1xminb	387	13	of	of	IN
cord-299281-5z1xminb	387	14	coronavirus	coronavirus	NN
cord-299281-5z1xminb	387	15	El	El	NNP
cord-299281-5z1xminb	387	16	protein	protein	NN
cord-299281-5z1xminb	387	17	.	.	.
cord-299281-5z1xminb	388	1	3	3	LS
cord-299281-5z1xminb	389	1	The	the	DT
cord-299281-5z1xminb	389	2	transmembrane	transmembrane	NN
cord-299281-5z1xminb	389	3	domain	domain	NN
cord-299281-5z1xminb	389	4	of	of	IN
cord-299281-5z1xminb	389	5	N	n	CD
cord-299281-5z1xminb	389	6	-	-	HYPH
cord-299281-5z1xminb	389	7	gincosaminyltransferase	gincosaminyltransferase	NNP
cord-299281-5z1xminb	390	1	I	-PRON-	PRP
cord-299281-5z1xminb	390	2	contains	contain	VBZ
cord-299281-5z1xminb	390	3	a	a	DT
cord-299281-5z1xminb	390	4	Golgi	Golgi	NNP
cord-299281-5z1xminb	390	5	retention	retention	NN
cord-299281-5z1xminb	390	6	signal	signal	NN
cord-299281-5z1xminb	391	1	The	the	DT
cord-299281-5z1xminb	391	2	signal	signal	NN
cord-299281-5z1xminb	391	3	for	for	IN
cord-299281-5z1xminb	391	4	Golgi	Golgi	NNP
cord-299281-5z1xminb	391	5	retention	retention	NN
cord-299281-5z1xminb	391	6	of	of	IN
cord-299281-5z1xminb	391	7	bovine	bovine	NN
cord-299281-5z1xminb	391	8	81,4-galactosyltrunsferase	81,4-galactosyltrunsferase	NN
cord-299281-5z1xminb	391	9	is	be	VBZ
cord-299281-5z1xminb	391	10	in	in	IN
cord-299281-5z1xminb	391	11	the	the	DT
cord-299281-5z1xminb	391	12	transmembrane	transmembrane	NN
cord-299281-5z1xminb	391	13	domain	domain	NN
cord-299281-5z1xminb	392	1	The	the	DT
cord-299281-5z1xminb	392	2	17-residue	17-residue	CD
cord-299281-5z1xminb	392	3	transmembrane	transmembrane	NN
cord-299281-5z1xminb	392	4	domain	domain	NN
cord-299281-5z1xminb	392	5	of	of	IN
cord-299281-5z1xminb	392	6	8-galactoside	8-galactoside	CD
cord-299281-5z1xminb	392	7	a2,6-sialyltransferase	a2,6-sialyltransferase	NFP
cord-299281-5z1xminb	392	8	is	be	VBZ
cord-299281-5z1xminb	392	9	sufficient	sufficient	JJ
cord-299281-5z1xminb	392	10	for	for	IN
cord-299281-5z1xminb	392	11	Golgi	Golgi	NNP
cord-299281-5z1xminb	392	12	retention	retention	NN