id	sid	tid	token	lemma	pos
cord-104268-q1jx0n0l	1	1	key	key	JJ
cord-104268-q1jx0n0l	1	2	:	:	:
cord-104268-q1jx0n0l	1	3	cord-104268-q1jx0n0l	cord-104268-q1jx0n0l	NNP
cord-104268-q1jx0n0l	1	4	authors	author	NNS
cord-104268-q1jx0n0l	1	5	:	:	:
cord-104268-q1jx0n0l	2	1	nan	nan	NNP
cord-104268-q1jx0n0l	2	2	title	title	NN
cord-104268-q1jx0n0l	2	3	:	:	:
cord-104268-q1jx0n0l	2	4	Localization	localization	NN
cord-104268-q1jx0n0l	2	5	and	and	CC
cord-104268-q1jx0n0l	2	6	targeting	targeting	NN
cord-104268-q1jx0n0l	2	7	of	of	IN
cord-104268-q1jx0n0l	2	8	the	the	DT
cord-104268-q1jx0n0l	2	9	Saccharomyces	saccharomyce	NNS
cord-104268-q1jx0n0l	3	1	cerevisiae	cerevisiae	NNP
cord-104268-q1jx0n0l	3	2	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	3	3	/	/	SYM
cord-104268-q1jx0n0l	3	4	Mnt1p	mnt1p	CC
cord-104268-q1jx0n0l	3	5	alpha	alpha	NN
cord-104268-q1jx0n0l	3	6	1,2-mannosyltransferase	1,2-mannosyltransferase	CD
cord-104268-q1jx0n0l	3	7	to	to	IN
cord-104268-q1jx0n0l	3	8	a	a	DT
cord-104268-q1jx0n0l	3	9	medial	medial	JJ
cord-104268-q1jx0n0l	3	10	-	-	HYPH
cord-104268-q1jx0n0l	3	11	Golgi	golgi	NN
cord-104268-q1jx0n0l	3	12	compartment	compartment	NN
cord-104268-q1jx0n0l	3	13	date	date	NN
cord-104268-q1jx0n0l	3	14	:	:	:
cord-104268-q1jx0n0l	3	15	1995	1995	CD
cord-104268-q1jx0n0l	3	16	-	-	SYM
cord-104268-q1jx0n0l	3	17	11	11	CD
cord-104268-q1jx0n0l	3	18	-	-	HYPH
cord-104268-q1jx0n0l	3	19	02	02	CD
cord-104268-q1jx0n0l	3	20	journal	journal	NN
cord-104268-q1jx0n0l	3	21	:	:	:
cord-104268-q1jx0n0l	4	1	J	J	NNP
cord-104268-q1jx0n0l	4	2	Cell	Cell	NNP
cord-104268-q1jx0n0l	4	3	Biol	Biol	NNP
cord-104268-q1jx0n0l	4	4	DOI	DOI	NNP
cord-104268-q1jx0n0l	4	5	:	:	:
cord-104268-q1jx0n0l	4	6	nan	nan	NNP
cord-104268-q1jx0n0l	4	7	sha	sha	NNP
cord-104268-q1jx0n0l	4	8	:	:	:
cord-104268-q1jx0n0l	4	9	e3f5b91907bbd807d31fa063a9d629e574b4dbde	e3f5b91907bbd807d31fa063a9d629e574b4dbde	NNP
cord-104268-q1jx0n0l	4	10	doc_id	doc_id	NNP
cord-104268-q1jx0n0l	4	11	:	:	:
cord-104268-q1jx0n0l	5	1	104268	104268	CD
cord-104268-q1jx0n0l	5	2	cord_uid	cord_uid	NNS
cord-104268-q1jx0n0l	5	3	:	:	:
cord-104268-q1jx0n0l	5	4	q1jx0n0l	q1jx0n0l	XX
cord-104268-q1jx0n0l	6	1	The	the	DT
cord-104268-q1jx0n0l	6	2	yeast	yeast	NN
cord-104268-q1jx0n0l	6	3	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	6	4	/	/	SYM
cord-104268-q1jx0n0l	6	5	Mnt1p	mnt1p	CC
cord-104268-q1jx0n0l	6	6	alpha	alpha	NN
cord-104268-q1jx0n0l	6	7	1,2-mannosyltransferase	1,2-mannosyltransferase	CD
cord-104268-q1jx0n0l	6	8	is	be	VBZ
cord-104268-q1jx0n0l	6	9	a	a	DT
cord-104268-q1jx0n0l	6	10	type	type	NN
cord-104268-q1jx0n0l	6	11	II	II	NNP
cord-104268-q1jx0n0l	6	12	membrane	membrane	NN
cord-104268-q1jx0n0l	6	13	protein	protein	NN
cord-104268-q1jx0n0l	6	14	with	with	IN
cord-104268-q1jx0n0l	6	15	a	a	DT
cord-104268-q1jx0n0l	6	16	short	short	JJ
cord-104268-q1jx0n0l	6	17	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	6	18	amino	amino	NN
cord-104268-q1jx0n0l	6	19	terminus	terminus	NN
cord-104268-q1jx0n0l	6	20	,	,	,
cord-104268-q1jx0n0l	6	21	a	a	DT
cord-104268-q1jx0n0l	6	22	membrane-	membrane-	JJ
cord-104268-q1jx0n0l	6	23	spanning	span	VBG
cord-104268-q1jx0n0l	6	24	region	region	NN
cord-104268-q1jx0n0l	6	25	,	,	,
cord-104268-q1jx0n0l	6	26	and	and	CC
cord-104268-q1jx0n0l	6	27	a	a	DT
cord-104268-q1jx0n0l	6	28	large	large	JJ
cord-104268-q1jx0n0l	6	29	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	6	30	luminal	luminal	JJ
cord-104268-q1jx0n0l	6	31	domain	domain	NN
cord-104268-q1jx0n0l	6	32	containing	contain	VBG
cord-104268-q1jx0n0l	6	33	one	one	CD
cord-104268-q1jx0n0l	6	34	N-	n-	CD
cord-104268-q1jx0n0l	6	35	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	6	36	site	site	NN
cord-104268-q1jx0n0l	6	37	.	.	.
cord-104268-q1jx0n0l	7	1	Anti	Anti	NNP
cord-104268-q1jx0n0l	7	2	-	-	NNP
cord-104268-q1jx0n0l	7	3	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	7	4	/	/	SYM
cord-104268-q1jx0n0l	7	5	Mnt1p	mnt1p	NN
cord-104268-q1jx0n0l	7	6	antibodies	antibody	NNS
cord-104268-q1jx0n0l	7	7	identify	identify	VBP
cord-104268-q1jx0n0l	7	8	a	a	DT
cord-104268-q1jx0n0l	7	9	60-kD	60-kd	CD
cord-104268-q1jx0n0l	7	10	integral	integral	JJ
cord-104268-q1jx0n0l	7	11	membrane	membrane	NN
cord-104268-q1jx0n0l	7	12	protein	protein	NN
cord-104268-q1jx0n0l	7	13	that	that	WDT
cord-104268-q1jx0n0l	7	14	is	be	VBZ
cord-104268-q1jx0n0l	7	15	progressively	progressively	RB
cord-104268-q1jx0n0l	7	16	N	n	CD
cord-104268-q1jx0n0l	7	17	-	-	HYPH
cord-104268-q1jx0n0l	7	18	glycosylated	glycosylate	VBN
cord-104268-q1jx0n0l	7	19	in	in	IN
cord-104268-q1jx0n0l	7	20	an	an	DT
cord-104268-q1jx0n0l	7	21	MNN1-dependent	MNN1-dependent	NNP
cord-104268-q1jx0n0l	7	22	manner	manner	NN
cord-104268-q1jx0n0l	7	23	.	.	.
cord-104268-q1jx0n0l	8	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	8	2	/	/	SYM
cord-104268-q1jx0n0l	8	3	Mnt1p	mnt1p	CC
cord-104268-q1jx0n0l	8	4	is	be	VBZ
cord-104268-q1jx0n0l	8	5	localized	localize	VBN
cord-104268-q1jx0n0l	8	6	in	in	IN
cord-104268-q1jx0n0l	8	7	a	a	DT
cord-104268-q1jx0n0l	8	8	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	8	9	compartment	compartment	NN
cord-104268-q1jx0n0l	8	10	that	that	WDT
cord-104268-q1jx0n0l	8	11	overlaps	overlap	VBZ
cord-104268-q1jx0n0l	8	12	with	with	IN
cord-104268-q1jx0n0l	8	13	that	that	DT
cord-104268-q1jx0n0l	8	14	containing	contain	VBG
cord-104268-q1jx0n0l	8	15	the	the	DT
cord-104268-q1jx0n0l	8	16	medial	medial	JJ
cord-104268-q1jx0n0l	8	17	-	-	HYPH
cord-104268-q1jx0n0l	8	18	Golgi	golgi	NN
cord-104268-q1jx0n0l	8	19	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	8	20	Mnn1p	Mnn1p	NNP
cord-104268-q1jx0n0l	8	21	,	,	,
cord-104268-q1jx0n0l	8	22	and	and	CC
cord-104268-q1jx0n0l	8	23	distinct	distinct	JJ
cord-104268-q1jx0n0l	8	24	from	from	IN
cord-104268-q1jx0n0l	8	25	that	that	IN
cord-104268-q1jx0n0l	8	26	including	include	VBG
cord-104268-q1jx0n0l	8	27	the	the	DT
cord-104268-q1jx0n0l	8	28	late	late	JJ
cord-104268-q1jx0n0l	8	29	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	8	30	protein	protein	NN
cord-104268-q1jx0n0l	8	31	Kex1p	Kex1p	NNP
cord-104268-q1jx0n0l	8	32	.	.	.
cord-104268-q1jx0n0l	9	1	To	to	TO
cord-104268-q1jx0n0l	9	2	determine	determine	VB
cord-104268-q1jx0n0l	9	3	which	which	WDT
cord-104268-q1jx0n0l	9	4	regions	region	NNS
cord-104268-q1jx0n0l	9	5	of	of	IN
cord-104268-q1jx0n0l	9	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	9	7	/	/	SYM
cord-104268-q1jx0n0l	9	8	Mnt1p	mnt1p	CC
cord-104268-q1jx0n0l	9	9	are	be	VBP
cord-104268-q1jx0n0l	9	10	required	require	VBN
cord-104268-q1jx0n0l	9	11	for	for	IN
cord-104268-q1jx0n0l	9	12	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	9	13	localization	localization	NN
cord-104268-q1jx0n0l	9	14	,	,	,
cord-104268-q1jx0n0l	9	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	9	16	/	/	SYM
cord-104268-q1jx0n0l	9	17	Mnt1p	mnt1p	NN
cord-104268-q1jx0n0l	9	18	mutant	mutant	JJ
cord-104268-q1jx0n0l	9	19	proteins	protein	NNS
cord-104268-q1jx0n0l	9	20	were	be	VBD
cord-104268-q1jx0n0l	9	21	assembled	assemble	VBN
cord-104268-q1jx0n0l	9	22	by	by	IN
cord-104268-q1jx0n0l	9	23	substitution	substitution	NN
cord-104268-q1jx0n0l	9	24	of	of	IN
cord-104268-q1jx0n0l	9	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	9	26	domains	domain	NNS
cord-104268-q1jx0n0l	9	27	with	with	IN
cord-104268-q1jx0n0l	9	28	equivalent	equivalent	JJ
cord-104268-q1jx0n0l	9	29	sequences	sequence	NNS
cord-104268-q1jx0n0l	9	30	from	from	IN
cord-104268-q1jx0n0l	9	31	the	the	DT
cord-104268-q1jx0n0l	9	32	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	9	33	proteins	protein	NNS
cord-104268-q1jx0n0l	9	34	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	9	35	B	B	NNP
cord-104268-q1jx0n0l	9	36	and	and	CC
cord-104268-q1jx0n0l	9	37	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	9	38	.	.	.
cord-104268-q1jx0n0l	10	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	10	2	proteins	protein	NNS
cord-104268-q1jx0n0l	10	3	were	be	VBD
cord-104268-q1jx0n0l	10	4	tested	test	VBN
cord-104268-q1jx0n0l	10	5	for	for	IN
cord-104268-q1jx0n0l	10	6	correct	correct	JJ
cord-104268-q1jx0n0l	10	7	topology	topology	NN
cord-104268-q1jx0n0l	10	8	,	,	,
cord-104268-q1jx0n0l	10	9	in	in	IN
cord-104268-q1jx0n0l	10	10	vitro	vitro	FW
cord-104268-q1jx0n0l	10	11	and	and	CC
cord-104268-q1jx0n0l	10	12	in	in	IN
cord-104268-q1jx0n0l	10	13	vivo	vivo	NN
cord-104268-q1jx0n0l	10	14	activity	activity	NN
cord-104268-q1jx0n0l	10	15	,	,	,
cord-104268-q1jx0n0l	10	16	and	and	CC
cord-104268-q1jx0n0l	10	17	were	be	VBD
cord-104268-q1jx0n0l	10	18	localized	localize	VBN
cord-104268-q1jx0n0l	10	19	intracellularly	intracellularly	RB
cord-104268-q1jx0n0l	10	20	by	by	IN
cord-104268-q1jx0n0l	10	21	indirect	indirect	JJ
cord-104268-q1jx0n0l	10	22	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	10	23	.	.	.
cord-104268-q1jx0n0l	11	1	The	the	DT
cord-104268-q1jx0n0l	11	2	results	result	NNS
cord-104268-q1jx0n0l	11	3	demonstrate	demonstrate	VBP
cord-104268-q1jx0n0l	11	4	that	that	IN
cord-104268-q1jx0n0l	11	5	the	the	DT
cord-104268-q1jx0n0l	11	6	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	11	7	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	11	8	domain	domain	NN
cord-104268-q1jx0n0l	11	9	is	be	VBZ
cord-104268-q1jx0n0l	11	10	necessary	necessary	JJ
cord-104268-q1jx0n0l	11	11	for	for	IN
cord-104268-q1jx0n0l	11	12	correct	correct	JJ
cord-104268-q1jx0n0l	11	13	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	11	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	11	15	localization	localization	NN
cord-104268-q1jx0n0l	11	16	whereas	whereas	RB
cord-104268-q1jx0n0l	11	17	,	,	,
cord-104268-q1jx0n0l	11	18	the	the	DT
cord-104268-q1jx0n0l	11	19	membrane	membrane	NN
cord-104268-q1jx0n0l	11	20	-	-	HYPH
cord-104268-q1jx0n0l	11	21	spanning	span	VBG
cord-104268-q1jx0n0l	11	22	and	and	CC
cord-104268-q1jx0n0l	11	23	stem	stem	NN
cord-104268-q1jx0n0l	11	24	domains	domain	NNS
cord-104268-q1jx0n0l	11	25	are	be	VBP
cord-104268-q1jx0n0l	11	26	dispensable	dispensable	JJ
cord-104268-q1jx0n0l	11	27	.	.	.
cord-104268-q1jx0n0l	12	1	However	however	RB
cord-104268-q1jx0n0l	12	2	,	,	,
cord-104268-q1jx0n0l	12	3	in	in	IN
cord-104268-q1jx0n0l	12	4	a	a	DT
cord-104268-q1jx0n0l	12	5	test	test	NN
cord-104268-q1jx0n0l	12	6	of	of	IN
cord-104268-q1jx0n0l	12	7	targeting	target	VBG
cord-104268-q1jx0n0l	12	8	sufficiency	sufficiency	NN
cord-104268-q1jx0n0l	12	9	,	,	,
cord-104268-q1jx0n0l	12	10	the	the	DT
cord-104268-q1jx0n0l	12	11	presence	presence	NN
cord-104268-q1jx0n0l	12	12	of	of	IN
cord-104268-q1jx0n0l	12	13	the	the	DT
cord-104268-q1jx0n0l	12	14	entire	entire	JJ
cord-104268-q1jx0n0l	12	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	12	16	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	12	17	tail	tail	NN
cord-104268-q1jx0n0l	12	18	,	,	,
cord-104268-q1jx0n0l	12	19	plus	plus	CC
cord-104268-q1jx0n0l	12	20	the	the	DT
cord-104268-q1jx0n0l	12	21	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	12	22	domain	domain	NN
cord-104268-q1jx0n0l	12	23	and	and	CC
cord-104268-q1jx0n0l	12	24	a	a	DT
cord-104268-q1jx0n0l	12	25	36-amino	36-amino	CD
cord-104268-q1jx0n0l	12	26	acid	acid	NN
cord-104268-q1jx0n0l	12	27	residue	residue	NN
cord-104268-q1jx0n0l	12	28	luminal	luminal	JJ
cord-104268-q1jx0n0l	12	29	stem	stem	NN
cord-104268-q1jx0n0l	12	30	region	region	NN
cord-104268-q1jx0n0l	12	31	was	be	VBD
cord-104268-q1jx0n0l	12	32	required	require	VBN
cord-104268-q1jx0n0l	12	33	to	to	TO
cord-104268-q1jx0n0l	12	34	localize	localize	VB
cord-104268-q1jx0n0l	12	35	a	a	DT
cord-104268-q1jx0n0l	12	36	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	12	37	reporter	reporter	NN
cord-104268-q1jx0n0l	12	38	protein	protein	NN
cord-104268-q1jx0n0l	12	39	to	to	IN
cord-104268-q1jx0n0l	12	40	the	the	DT
cord-104268-q1jx0n0l	12	41	yeast	yeast	NN
cord-104268-q1jx0n0l	12	42	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	12	43	.	.	.
cord-104268-q1jx0n0l	13	1	cesses	cesse	NNS
cord-104268-q1jx0n0l	13	2	have	have	VBP
cord-104268-q1jx0n0l	13	3	been	be	VBN
cord-104268-q1jx0n0l	13	4	studied	study	VBN
cord-104268-q1jx0n0l	13	5	from	from	IN
cord-104268-q1jx0n0l	13	6	genetic	genetic	JJ
cord-104268-q1jx0n0l	13	7	and	and	CC
cord-104268-q1jx0n0l	13	8	biochemical	biochemical	JJ
cord-104268-q1jx0n0l	13	9	perspectives	perspective	NNS
cord-104268-q1jx0n0l	13	10	(	(	-LRB-
cord-104268-q1jx0n0l	13	11	Kukuruzinska	Kukuruzinska	NNP
cord-104268-q1jx0n0l	13	12	et	et	FW
cord-104268-q1jx0n0l	13	13	al	al	NNP
cord-104268-q1jx0n0l	13	14	.	.	.
cord-104268-q1jx0n0l	14	1	,	,	,
cord-104268-q1jx0n0l	14	2	1987	1987	CD
cord-104268-q1jx0n0l	14	3	;	;	:
cord-104268-q1jx0n0l	14	4	Pryer	Pryer	NNP
cord-104268-q1jx0n0l	14	5	et	et	NNP
cord-104268-q1jx0n0l	14	6	al	al	NNP
cord-104268-q1jx0n0l	14	7	.	.	NNP
cord-104268-q1jx0n0l	14	8	,	,	,
cord-104268-q1jx0n0l	14	9	1992	1992	CD
cord-104268-q1jx0n0l	14	10	)	)	-RRB-
cord-104268-q1jx0n0l	14	11	resulting	result	VBG
cord-104268-q1jx0n0l	14	12	in	in	IN
cord-104268-q1jx0n0l	14	13	a	a	DT
cord-104268-q1jx0n0l	14	14	functionally	functionally	RB
cord-104268-q1jx0n0l	14	15	defined	define	VBN
cord-104268-q1jx0n0l	14	16	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	14	17	apparatus	apparatus	NN
cord-104268-q1jx0n0l	14	18	that	that	WDT
cord-104268-q1jx0n0l	14	19	has	have	VBZ
cord-104268-q1jx0n0l	14	20	been	be	VBN
cord-104268-q1jx0n0l	14	21	subdivided	subdivide	VBN
cord-104268-q1jx0n0l	14	22	into	into	IN
cord-104268-q1jx0n0l	14	23	several	several	JJ
cord-104268-q1jx0n0l	14	24	subcompartments	subcompartment	NNS
cord-104268-q1jx0n0l	14	25	on	on	IN
cord-104268-q1jx0n0l	14	26	the	the	DT
cord-104268-q1jx0n0l	14	27	basis	basis	NN
cord-104268-q1jx0n0l	14	28	of	of	IN
cord-104268-q1jx0n0l	14	29	asparagine	asparagine	NN
cord-104268-q1jx0n0l	14	30	-	-	HYPH
cord-104268-q1jx0n0l	14	31	linked	link	VBN
cord-104268-q1jx0n0l	14	32	(	(	-LRB-
cord-104268-q1jx0n0l	14	33	N	n	NN
cord-104268-q1jx0n0l	14	34	-	-	HYPH
cord-104268-q1jx0n0l	14	35	linked	link	VBN
cord-104268-q1jx0n0l	14	36	)	)	-RRB-
cord-104268-q1jx0n0l	14	37	1	1	CD
cord-104268-q1jx0n0l	14	38	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	14	39	modifications	modification	NNS
cord-104268-q1jx0n0l	14	40	(	(	-LRB-
cord-104268-q1jx0n0l	14	41	Franzusoff	Franzusoff	NNP
cord-104268-q1jx0n0l	14	42	and	and	CC
cord-104268-q1jx0n0l	14	43	Schekman	Schekman	NNP
cord-104268-q1jx0n0l	14	44	,	,	,
cord-104268-q1jx0n0l	14	45	1989	1989	CD
cord-104268-q1jx0n0l	14	46	;	;	:
cord-104268-q1jx0n0l	14	47	Graham	Graham	NNP
cord-104268-q1jx0n0l	14	48	and	and	CC
cord-104268-q1jx0n0l	14	49	Emr	Emr	NNP
cord-104268-q1jx0n0l	14	50	,	,	,
cord-104268-q1jx0n0l	14	51	1991	1991	CD
cord-104268-q1jx0n0l	14	52	;	;	:
cord-104268-q1jx0n0l	14	53	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	14	54	and	and	CC
cord-104268-q1jx0n0l	14	55	Payne	Payne	NNP
cord-104268-q1jx0n0l	14	56	,	,	,
cord-104268-q1jx0n0l	14	57	1993	1993	CD
cord-104268-q1jx0n0l	14	58	;	;	:
cord-104268-q1jx0n0l	14	59	Gaynor	Gaynor	NNP
cord-104268-q1jx0n0l	14	60	et	et	NNP
cord-104268-q1jx0n0l	14	61	al	al	NNP
cord-104268-q1jx0n0l	14	62	.	.	NNP
cord-104268-q1jx0n0l	14	63	,	,	,
cord-104268-q1jx0n0l	14	64	1994	1994	CD
cord-104268-q1jx0n0l	14	65	)	)	-RRB-
cord-104268-q1jx0n0l	14	66	.	.	.
cord-104268-q1jx0n0l	15	1	In	in	IN
cord-104268-q1jx0n0l	15	2	yeast	yeast	NN
cord-104268-q1jx0n0l	15	3	,	,	,
cord-104268-q1jx0n0l	15	4	secretory	secretory	JJ
cord-104268-q1jx0n0l	15	5	pathway	pathway	NN
cord-104268-q1jx0n0l	15	6	glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	15	7	can	can	MD
cord-104268-q1jx0n0l	15	8	acquire	acquire	VB
cord-104268-q1jx0n0l	15	9	two	two	CD
cord-104268-q1jx0n0l	15	10	types	type	NNS
cord-104268-q1jx0n0l	15	11	of	of	IN
cord-104268-q1jx0n0l	15	12	N	n	CD
cord-104268-q1jx0n0l	15	13	-	-	HYPH
cord-104268-q1jx0n0l	15	14	linked	link	VBN
cord-104268-q1jx0n0l	15	15	oligosaccharides	oligosaccharide	NNS
cord-104268-q1jx0n0l	15	16	,	,	,
cord-104268-q1jx0n0l	15	17	either	either	CC
cord-104268-q1jx0n0l	15	18	a	a	DT
cord-104268-q1jx0n0l	15	19	simple	simple	JJ
cord-104268-q1jx0n0l	15	20	core	core	NN
cord-104268-q1jx0n0l	15	21	carbohydrate	carbohydrate	NN
cord-104268-q1jx0n0l	15	22	or	or	CC
cord-104268-q1jx0n0l	15	23	one	one	CD
cord-104268-q1jx0n0l	15	24	extended	extend	VBN
cord-104268-q1jx0n0l	15	25	by	by	IN
cord-104268-q1jx0n0l	15	26	an	an	DT
cord-104268-q1jx0n0l	15	27	outer	outer	JJ
cord-104268-q1jx0n0l	15	28	-	-	HYPH
cord-104268-q1jx0n0l	15	29	chain	chain	NN
cord-104268-q1jx0n0l	15	30	glycan	glycan	NN
cord-104268-q1jx0n0l	15	31	structure	structure	NN
cord-104268-q1jx0n0l	15	32	.	.	.
cord-104268-q1jx0n0l	16	1	The	the	DT
cord-104268-q1jx0n0l	16	2	N	N	NNP
cord-104268-q1jx0n0l	16	3	-	-	HYPH
cord-104268-q1jx0n0l	16	4	linked	link	VBN
cord-104268-q1jx0n0l	16	5	core	core	NN
cord-104268-q1jx0n0l	16	6	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	16	7	elaborated	elaborate	VBD
cord-104268-q1jx0n0l	16	8	in	in	IN
cord-104268-q1jx0n0l	16	9	the	the	DT
cord-104268-q1jx0n0l	16	10	ER	ER	NNP
cord-104268-q1jx0n0l	16	11	is	be	VBZ
cord-104268-q1jx0n0l	16	12	mainly	mainly	RB
cord-104268-q1jx0n0l	16	13	constituted	constitute	VBN
cord-104268-q1jx0n0l	16	14	of	of	IN
cord-104268-q1jx0n0l	16	15	MansGlcNAc2	MansGlcNAc2	NNS
cord-104268-q1jx0n0l	16	16	and	and	CC
cord-104268-q1jx0n0l	16	17	may	may	MD
cord-104268-q1jx0n0l	16	18	undergo	undergo	VB
cord-104268-q1jx0n0l	16	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	16	20	maturation	maturation	NN
cord-104268-q1jx0n0l	16	21	resulting	result	VBG
cord-104268-q1jx0n0l	16	22	in	in	IN
cord-104268-q1jx0n0l	16	23	Man9.13	Man9.13	NNP
cord-104268-q1jx0n0l	16	24	_	_	NNP
cord-104268-q1jx0n0l	16	25	GlcNAc2	GlcNAc2	NNP
cord-104268-q1jx0n0l	16	26	.	.	.
cord-104268-q1jx0n0l	17	1	In	in	IN
cord-104268-q1jx0n0l	17	2	other	other	JJ
cord-104268-q1jx0n0l	17	3	cases	case	NNS
cord-104268-q1jx0n0l	17	4	,	,	,
cord-104268-q1jx0n0l	17	5	glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	17	6	traversing	traverse	VBG
cord-104268-q1jx0n0l	17	7	the	the	DT
cord-104268-q1jx0n0l	17	8	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	17	9	apparatus	apparatus	NN
cord-104268-q1jx0n0l	17	10	have	have	VBP
cord-104268-q1jx0n0l	17	11	their	-PRON-	PRP$
cord-104268-q1jx0n0l	17	12	core	core	NN
cord-104268-q1jx0n0l	17	13	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	17	14	extended	extend	VBN
cord-104268-q1jx0n0l	17	15	by	by	IN
cord-104268-q1jx0n0l	17	16	outer	outer	JJ
cord-104268-q1jx0n0l	17	17	chains	chain	NNS
cord-104268-q1jx0n0l	17	18	(	(	-LRB-
cord-104268-q1jx0n0l	17	19	see	see	VB
cord-104268-q1jx0n0l	17	20	Fig	Fig	NNP
cord-104268-q1jx0n0l	17	21	.	.	NNP
cord-104268-q1jx0n0l	17	22	2	2	CD
cord-104268-q1jx0n0l	17	23	;	;	:
cord-104268-q1jx0n0l	17	24	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	17	25	,	,	,
cord-104268-q1jx0n0l	17	26	1990	1990	CD
cord-104268-q1jx0n0l	17	27	;	;	:
cord-104268-q1jx0n0l	17	28	Herscovics	Herscovics	NNP
cord-104268-q1jx0n0l	17	29	and	and	CC
cord-104268-q1jx0n0l	17	30	Orlean	Orlean	NNP
cord-104268-q1jx0n0l	17	31	,	,	,
cord-104268-q1jx0n0l	17	32	1993	1993	CD
cord-104268-q1jx0n0l	17	33	)	)	-RRB-
cord-104268-q1jx0n0l	17	34	.	.	.
cord-104268-q1jx0n0l	18	1	The	the	DT
cord-104268-q1jx0n0l	18	2	initial	initial	JJ
cord-104268-q1jx0n0l	18	3	step	step	NN
cord-104268-q1jx0n0l	18	4	in	in	IN
cord-104268-q1jx0n0l	18	5	outer	outer	JJ
cord-104268-q1jx0n0l	18	6	-	-	HYPH
cord-104268-q1jx0n0l	18	7	chain	chain	NN
cord-104268-q1jx0n0l	18	8	synthesis	synthesis	NN
cord-104268-q1jx0n0l	18	9	takes	take	VBZ
cord-104268-q1jx0n0l	18	10	place	place	NN
cord-104268-q1jx0n0l	18	11	and	and	CC
cord-104268-q1jx0n0l	18	12	defines	define	VBZ
cord-104268-q1jx0n0l	18	13	an	an	DT
cord-104268-q1jx0n0l	18	14	early	early	JJ
cord-104268-q1jx0n0l	18	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	18	16	compartment	compartment	NN
cord-104268-q1jx0n0l	18	17	in	in	IN
cord-104268-q1jx0n0l	18	18	which	which	WDT
cord-104268-q1jx0n0l	18	19	a	a	DT
cord-104268-q1jx0n0l	18	20	backbone	backbone	NN
cord-104268-q1jx0n0l	18	21	of	of	IN
cord-104268-q1jx0n0l	18	22	ed,6	ed,6	NNP
cord-104268-q1jx0n0l	18	23	-	-	HYPH
cord-104268-q1jx0n0l	18	24	1inked	1inked	CD
cord-104268-q1jx0n0l	18	25	mannose	mannose	NN
cord-104268-q1jx0n0l	18	26	residues	residue	NNS
cord-104268-q1jx0n0l	18	27	is	be	VBZ
cord-104268-q1jx0n0l	18	28	attached	attach	VBN
cord-104268-q1jx0n0l	18	29	to	to	IN
cord-104268-q1jx0n0l	18	30	the	the	DT
cord-104268-q1jx0n0l	18	31	core	core	NN
cord-104268-q1jx0n0l	18	32	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	18	33	.	.	.
cord-104268-q1jx0n0l	19	1	This	this	DT
cord-104268-q1jx0n0l	19	2	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	19	3	compartment	compartment	NN
cord-104268-q1jx0n0l	19	4	has	have	VBZ
cord-104268-q1jx0n0l	19	5	been	be	VBN
cord-104268-q1jx0n0l	19	6	likened	liken	VBN
cord-104268-q1jx0n0l	19	7	to	to	IN
cord-104268-q1jx0n0l	19	8	the	the	DT
cord-104268-q1jx0n0l	19	9	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	19	10	cis	cis	NNP
cord-104268-q1jx0n0l	19	11	-	-	HYPH
cord-104268-q1jx0n0l	19	12	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	19	13	network	network	NN
cord-104268-q1jx0n0l	19	14	(	(	-LRB-
cord-104268-q1jx0n0l	19	15	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	19	16	and	and	CC
cord-104268-q1jx0n0l	19	17	Payne	Payne	NNP
cord-104268-q1jx0n0l	19	18	,	,	,
cord-104268-q1jx0n0l	19	19	1993	1993	CD
cord-104268-q1jx0n0l	19	20	)	)	-RRB-
cord-104268-q1jx0n0l	19	21	.	.	.
cord-104268-q1jx0n0l	20	1	Outer	outer	JJ
cord-104268-q1jx0n0l	20	2	chain	chain	NN
cord-104268-q1jx0n0l	20	3	elaboration	elaboration	NN
cord-104268-q1jx0n0l	20	4	is	be	VBZ
cord-104268-q1jx0n0l	20	5	then	then	RB
cord-104268-q1jx0n0l	20	6	brought	bring	VBN
cord-104268-q1jx0n0l	20	7	to	to	IN
cord-104268-q1jx0n0l	20	8	completion	completion	NN
cord-104268-q1jx0n0l	20	9	:	:	:
cord-104268-q1jx0n0l	20	10	the	the	DT
cord-104268-q1jx0n0l	20	11	al,6	al,6	NNP
cord-104268-q1jx0n0l	20	12	-	-	HYPH
cord-104268-q1jx0n0l	20	13	1inked	1inked	CD
cord-104268-q1jx0n0l	20	14	mannose	mannose	NN
cord-104268-q1jx0n0l	20	15	structure	structure	NN
cord-104268-q1jx0n0l	20	16	is	be	VBZ
cord-104268-q1jx0n0l	20	17	enlarged	enlarge	VBN
cord-104268-q1jx0n0l	20	18	in	in	IN
cord-104268-q1jx0n0l	20	19	a	a	DT
cord-104268-q1jx0n0l	20	20	sequential	sequential	JJ
cord-104268-q1jx0n0l	20	21	fashion	fashion	NN
cord-104268-q1jx0n0l	20	22	by	by	IN
cord-104268-q1jx0n0l	20	23	al,2-and	al,2-and	NNP
cord-104268-q1jx0n0l	20	24	al,3-mannosyltransferases	al,3-mannosyltransferases	XX
cord-104268-q1jx0n0l	20	25	in	in	IN
cord-104268-q1jx0n0l	20	26	a	a	DT
cord-104268-q1jx0n0l	20	27	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	20	28	distinct	distinct	JJ
cord-104268-q1jx0n0l	20	29	com	com	NN
cord-104268-q1jx0n0l	20	30	-	-	HYPH
cord-104268-q1jx0n0l	20	31	partment	partment	NN
cord-104268-q1jx0n0l	20	32	defining	define	VBG
cord-104268-q1jx0n0l	20	33	the	the	DT
cord-104268-q1jx0n0l	20	34	yeast	yeast	NN
cord-104268-q1jx0n0l	20	35	medial	medial	JJ
cord-104268-q1jx0n0l	20	36	-	-	HYPH
cord-104268-q1jx0n0l	20	37	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	20	38	compartment	compartment	NN
cord-104268-q1jx0n0l	20	39	.	.	.
cord-104268-q1jx0n0l	21	1	Mature	mature	JJ
cord-104268-q1jx0n0l	21	2	glycosylated	glycosylated	JJ
cord-104268-q1jx0n0l	21	3	proteins	protein	NNS
cord-104268-q1jx0n0l	21	4	are	be	VBP
cord-104268-q1jx0n0l	21	5	finally	finally	RB
cord-104268-q1jx0n0l	21	6	transported	transport	VBN
cord-104268-q1jx0n0l	21	7	to	to	IN
cord-104268-q1jx0n0l	21	8	a	a	DT
cord-104268-q1jx0n0l	21	9	late	late	JJ
cord-104268-q1jx0n0l	21	10	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	21	11	compartment	compartment	NN
cord-104268-q1jx0n0l	21	12	where	where	WRB
cord-104268-q1jx0n0l	21	13	proteolytic	proteolytic	JJ
cord-104268-q1jx0n0l	21	14	processing	processing	NN
cord-104268-q1jx0n0l	21	15	of	of	IN
cord-104268-q1jx0n0l	21	16	secreted	secrete	VBN
cord-104268-q1jx0n0l	21	17	protein	protein	NN
cord-104268-q1jx0n0l	21	18	precursors	precursor	NNS
cord-104268-q1jx0n0l	21	19	occurs	occur	VBZ
cord-104268-q1jx0n0l	21	20	.	.	.
cord-104268-q1jx0n0l	22	1	Some	some	DT
cord-104268-q1jx0n0l	22	2	evidence	evidence	NN
cord-104268-q1jx0n0l	22	3	suggests	suggest	VBZ
cord-104268-q1jx0n0l	22	4	that	that	IN
cord-104268-q1jx0n0l	22	5	this	this	DT
cord-104268-q1jx0n0l	22	6	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	22	7	subcompartment	subcompartment	NN
cord-104268-q1jx0n0l	22	8	is	be	VBZ
cord-104268-q1jx0n0l	22	9	also	also	RB
cord-104268-q1jx0n0l	22	10	involved	involve	VBN
cord-104268-q1jx0n0l	22	11	in	in	IN
cord-104268-q1jx0n0l	22	12	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	22	13	protein	protein	NN
cord-104268-q1jx0n0l	22	14	sorting	sorting	NN
cord-104268-q1jx0n0l	22	15	,	,	,
cord-104268-q1jx0n0l	22	16	making	make	VBG
cord-104268-q1jx0n0l	22	17	this	this	DT
cord-104268-q1jx0n0l	22	18	late	late	JJ
cord-104268-q1jx0n0l	22	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	22	20	compartment	compartment	NN
cord-104268-q1jx0n0l	22	21	comparable	comparable	JJ
cord-104268-q1jx0n0l	22	22	to	to	IN
cord-104268-q1jx0n0l	22	23	the	the	DT
cord-104268-q1jx0n0l	22	24	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	22	25	TGN	TGN	NNP
cord-104268-q1jx0n0l	22	26	(	(	-LRB-
cord-104268-q1jx0n0l	22	27	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	22	28	and	and	CC
cord-104268-q1jx0n0l	22	29	Payne	Payne	NNP
cord-104268-q1jx0n0l	22	30	,	,	,
cord-104268-q1jx0n0l	22	31	1993	1993	CD
cord-104268-q1jx0n0l	22	32	)	)	-RRB-
cord-104268-q1jx0n0l	22	33	.	.	.
cord-104268-q1jx0n0l	23	1	In	in	IN
cord-104268-q1jx0n0l	23	2	S.	S.	NNP
cord-104268-q1jx0n0l	23	3	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	23	4	,	,	,
cord-104268-q1jx0n0l	23	5	the	the	DT
cord-104268-q1jx0n0l	23	6	various	various	JJ
cord-104268-q1jx0n0l	23	7	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	23	8	compartments	compartment	NNS
cord-104268-q1jx0n0l	23	9	have	have	VBP
cord-104268-q1jx0n0l	23	10	not	not	RB
cord-104268-q1jx0n0l	23	11	been	be	VBN
cord-104268-q1jx0n0l	23	12	morphologically	morphologically	RB
cord-104268-q1jx0n0l	23	13	well	well	RB
cord-104268-q1jx0n0l	23	14	characterized	characterize	VBN
cord-104268-q1jx0n0l	23	15	.	.	.
cord-104268-q1jx0n0l	24	1	The	the	DT
cord-104268-q1jx0n0l	24	2	yeast	yeast	NN
cord-104268-q1jx0n0l	24	3	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	24	4	complex	complex	NN
cord-104268-q1jx0n0l	24	5	is	be	VBZ
cord-104268-q1jx0n0l	24	6	not	not	RB
cord-104268-q1jx0n0l	24	7	structurally	structurally	RB
cord-104268-q1jx0n0l	24	8	similar	similar	JJ
cord-104268-q1jx0n0l	24	9	to	to	IN
cord-104268-q1jx0n0l	24	10	the	the	DT
cord-104268-q1jx0n0l	24	11	perinuclear	perinuclear	NNP
cord-104268-q1jx0n0l	24	12	stacked	stack	VBD
cord-104268-q1jx0n0l	24	13	cisternal	cisternal	JJ
cord-104268-q1jx0n0l	24	14	subcompartments	subcompartment	NNS
cord-104268-q1jx0n0l	24	15	characteristic	characteristic	NN
cord-104268-q1jx0n0l	24	16	of	of	IN
cord-104268-q1jx0n0l	24	17	the	the	DT
cord-104268-q1jx0n0l	24	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	24	19	apparatus	apparatus	NN
cord-104268-q1jx0n0l	24	20	of	of	IN
cord-104268-q1jx0n0l	24	21	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	24	22	cells	cell	NNS
cord-104268-q1jx0n0l	24	23	.	.	.
cord-104268-q1jx0n0l	25	1	Immunoelectron	Immunoelectron	NNP
cord-104268-q1jx0n0l	25	2	microscopy	microscopy	NN
cord-104268-q1jx0n0l	25	3	revealed	reveal	VBD
cord-104268-q1jx0n0l	25	4	that	that	IN
cord-104268-q1jx0n0l	25	5	the	the	DT
cord-104268-q1jx0n0l	25	6	yeast	yeast	NN
cord-104268-q1jx0n0l	25	7	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	25	8	is	be	VBZ
cord-104268-q1jx0n0l	25	9	composed	compose	VBN
cord-104268-q1jx0n0l	25	10	of	of	IN
cord-104268-q1jx0n0l	25	11	as	as	RB
cord-104268-q1jx0n0l	25	12	many	many	JJ
cord-104268-q1jx0n0l	25	13	as	as	IN
cord-104268-q1jx0n0l	25	14	30	30	CD
cord-104268-q1jx0n0l	25	15	concave	concave	NN
cord-104268-q1jx0n0l	25	16	,	,	,
cord-104268-q1jx0n0l	25	17	disklike	disklike	NNP
cord-104268-q1jx0n0l	25	18	membranous	membranous	JJ
cord-104268-q1jx0n0l	25	19	cisternae	cisternae	NNP
cord-104268-q1jx0n0l	25	20	scattered	scatter	VBN
cord-104268-q1jx0n0l	25	21	in	in	IN
cord-104268-q1jx0n0l	25	22	the	the	DT
cord-104268-q1jx0n0l	25	23	cytoplasm	cytoplasm	NN
cord-104268-q1jx0n0l	25	24	and	and	CC
cord-104268-q1jx0n0l	25	25	most	most	RBS
cord-104268-q1jx0n0l	25	26	often	often	RB
cord-104268-q1jx0n0l	25	27	not	not	RB
cord-104268-q1jx0n0l	25	28	organized	organize	VBN
cord-104268-q1jx0n0l	25	29	in	in	IN
cord-104268-q1jx0n0l	25	30	parallel	parallel	JJ
cord-104268-q1jx0n0l	25	31	stacks	stack	NNS
cord-104268-q1jx0n0l	25	32	(	(	-LRB-
cord-104268-q1jx0n0l	25	33	Preuss	Preuss	NNP
cord-104268-q1jx0n0l	25	34	et	et	FW
cord-104268-q1jx0n0l	25	35	al	al	NNP
cord-104268-q1jx0n0l	25	36	.	.	NNP
cord-104268-q1jx0n0l	25	37	,	,	,
cord-104268-q1jx0n0l	25	38	1992	1992	CD
cord-104268-q1jx0n0l	25	39	)	)	-RRB-
cord-104268-q1jx0n0l	25	40	.	.	.
cord-104268-q1jx0n0l	26	1	In	in	IN
cord-104268-q1jx0n0l	26	2	indirect	indirect	JJ
cord-104268-q1jx0n0l	26	3	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	26	4	using	use	VBG
cord-104268-q1jx0n0l	26	5	antibodies	antibody	NNS
cord-104268-q1jx0n0l	26	6	to	to	IN
cord-104268-q1jx0n0l	26	7	different	different	JJ
cord-104268-q1jx0n0l	26	8	yeast	yeast	NN
cord-104268-q1jx0n0l	26	9	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	26	10	proteins	protein	NNS
cord-104268-q1jx0n0l	26	11	,	,	,
cord-104268-q1jx0n0l	26	12	the	the	DT
cord-104268-q1jx0n0l	26	13	isolated	isolated	JJ
cord-104268-q1jx0n0l	26	14	cisternae	cisternae	NNP
cord-104268-q1jx0n0l	26	15	are	be	VBP
cord-104268-q1jx0n0l	26	16	visualized	visualize	VBN
cord-104268-q1jx0n0l	26	17	as	as	IN
cord-104268-q1jx0n0l	26	18	dispersed	disperse	VBN
cord-104268-q1jx0n0l	26	19	punctate	punctate	NN
cord-104268-q1jx0n0l	26	20	spots	spot	NNS
cord-104268-q1jx0n0l	26	21	(	(	-LRB-
cord-104268-q1jx0n0l	26	22	Franzusoff	Franzusoff	NNP
cord-104268-q1jx0n0l	26	23	et	et	NNP
cord-104268-q1jx0n0l	26	24	al	al	NNP
cord-104268-q1jx0n0l	26	25	.	.	NNP
cord-104268-q1jx0n0l	26	26	,	,	,
cord-104268-q1jx0n0l	26	27	1991	1991	CD
cord-104268-q1jx0n0l	26	28	;	;	:
cord-104268-q1jx0n0l	26	29	Redding	Redding	NNP
cord-104268-q1jx0n0l	26	30	et	et	NNP
cord-104268-q1jx0n0l	26	31	al	al	NNP
cord-104268-q1jx0n0l	26	32	.	.	NNP
cord-104268-q1jx0n0l	26	33	,	,	,
cord-104268-q1jx0n0l	26	34	1991	1991	CD
cord-104268-q1jx0n0l	26	35	;	;	:
cord-104268-q1jx0n0l	26	36	Antebi	Antebi	NNP
cord-104268-q1jx0n0l	26	37	and	and	CC
cord-104268-q1jx0n0l	26	38	Fink	Fink	NNP
cord-104268-q1jx0n0l	26	39	,	,	,
cord-104268-q1jx0n0l	26	40	1992	1992	CD
cord-104268-q1jx0n0l	26	41	;	;	:
cord-104268-q1jx0n0l	26	42	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	26	43	and	and	CC
cord-104268-q1jx0n0l	26	44	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	26	45	,	,	,
cord-104268-q1jx0n0l	26	46	1992	1992	CD
cord-104268-q1jx0n0l	26	47	;	;	:
cord-104268-q1jx0n0l	26	48	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	26	49	et	et	NNP
cord-104268-q1jx0n0l	26	50	al	al	NNP
cord-104268-q1jx0n0l	26	51	.	.	NNP
cord-104268-q1jx0n0l	26	52	,	,	,
cord-104268-q1jx0n0l	26	53	1992	1992	CD
cord-104268-q1jx0n0l	26	54	)	)	-RRB-
cord-104268-q1jx0n0l	26	55	.	.	.
cord-104268-q1jx0n0l	27	1	The	the	DT
cord-104268-q1jx0n0l	27	2	establishment	establishment	NN
cord-104268-q1jx0n0l	27	3	and	and	CC
cord-104268-q1jx0n0l	27	4	maintenance	maintenance	NN
cord-104268-q1jx0n0l	27	5	of	of	IN
cord-104268-q1jx0n0l	27	6	the	the	DT
cord-104268-q1jx0n0l	27	7	polarized	polarized	JJ
cord-104268-q1jx0n0l	27	8	organization	organization	NN
cord-104268-q1jx0n0l	27	9	of	of	IN
cord-104268-q1jx0n0l	27	10	the	the	DT
cord-104268-q1jx0n0l	27	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	27	12	apparatus	apparatus	NN
cord-104268-q1jx0n0l	27	13	relies	rely	VBZ
cord-104268-q1jx0n0l	27	14	on	on	IN
cord-104268-q1jx0n0l	27	15	the	the	DT
cord-104268-q1jx0n0l	27	16	existence	existence	NN
cord-104268-q1jx0n0l	27	17	of	of	IN
cord-104268-q1jx0n0l	27	18	complex	complex	JJ
cord-104268-q1jx0n0l	27	19	sorting	sorting	NN
cord-104268-q1jx0n0l	27	20	and	and	CC
cord-104268-q1jx0n0l	27	21	transport	transport	NN
cord-104268-q1jx0n0l	27	22	mechanisms	mechanism	NNS
cord-104268-q1jx0n0l	27	23	permitting	permit	VBG
cord-104268-q1jx0n0l	27	24	specific	specific	JJ
cord-104268-q1jx0n0l	27	25	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	27	26	proteins	protein	NNS
cord-104268-q1jx0n0l	27	27	to	to	TO
cord-104268-q1jx0n0l	27	28	be	be	VB
cord-104268-q1jx0n0l	27	29	delivered	deliver	VBN
cord-104268-q1jx0n0l	27	30	to	to	IN
cord-104268-q1jx0n0l	27	31	their	-PRON-	PRP$
cord-104268-q1jx0n0l	27	32	precise	precise	JJ
cord-104268-q1jx0n0l	27	33	cisternal	cisternal	JJ
cord-104268-q1jx0n0l	27	34	destinations	destination	NNS
cord-104268-q1jx0n0l	27	35	.	.	.
cord-104268-q1jx0n0l	28	1	Resident	resident	JJ
cord-104268-q1jx0n0l	28	2	proteins	protein	NNS
cord-104268-q1jx0n0l	28	3	of	of	IN
cord-104268-q1jx0n0l	28	4	the	the	DT
cord-104268-q1jx0n0l	28	5	secretory	secretory	JJ
cord-104268-q1jx0n0l	28	6	pathway	pathway	NN
cord-104268-q1jx0n0l	28	7	of	of	IN
cord-104268-q1jx0n0l	28	8	eukaryotic	eukaryotic	JJ
cord-104268-q1jx0n0l	28	9	cells	cell	NNS
cord-104268-q1jx0n0l	28	10	require	require	VBP
cord-104268-q1jx0n0l	28	11	particular	particular	JJ
cord-104268-q1jx0n0l	28	12	targeting	targeting	NN
cord-104268-q1jx0n0l	28	13	signals	signal	NNS
cord-104268-q1jx0n0l	28	14	that	that	WDT
cord-104268-q1jx0n0l	28	15	specify	specify	VBP
cord-104268-q1jx0n0l	28	16	their	-PRON-	PRP$
cord-104268-q1jx0n0l	28	17	final	final	JJ
cord-104268-q1jx0n0l	28	18	location	location	NN
cord-104268-q1jx0n0l	28	19	(	(	-LRB-
cord-104268-q1jx0n0l	28	20	Pelham	Pelham	NNP
cord-104268-q1jx0n0l	28	21	and	and	CC
cord-104268-q1jx0n0l	28	22	Munro	Munro	NNP
cord-104268-q1jx0n0l	28	23	,	,	,
cord-104268-q1jx0n0l	28	24	1993	1993	CD
cord-104268-q1jx0n0l	28	25	;	;	:
cord-104268-q1jx0n0l	28	26	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	28	27	et	et	NNP
cord-104268-q1jx0n0l	28	28	al	al	NNP
cord-104268-q1jx0n0l	28	29	.	.	NNP
cord-104268-q1jx0n0l	28	30	,	,	,
cord-104268-q1jx0n0l	28	31	1994	1994	CD
cord-104268-q1jx0n0l	28	32	;	;	:
cord-104268-q1jx0n0l	28	33	Low	Low	NNP
cord-104268-q1jx0n0l	28	34	and	and	CC
cord-104268-q1jx0n0l	28	35	Hong	Hong	NNP
cord-104268-q1jx0n0l	28	36	,	,	,
cord-104268-q1jx0n0l	28	37	1994	1994	CD
cord-104268-q1jx0n0l	28	38	)	)	-RRB-
cord-104268-q1jx0n0l	28	39	.	.	.
cord-104268-q1jx0n0l	29	1	Mammalian	mammalian	JJ
cord-104268-q1jx0n0l	29	2	membrane	membrane	NN
cord-104268-q1jx0n0l	29	3	glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	29	4	not	not	RB
cord-104268-q1jx0n0l	29	5	possessing	possess	VBG
cord-104268-q1jx0n0l	29	6	positive	positive	JJ
cord-104268-q1jx0n0l	29	7	sorting	sorting	NN
cord-104268-q1jx0n0l	29	8	sequences	sequence	NNS
cord-104268-q1jx0n0l	29	9	are	be	VBP
cord-104268-q1jx0n0l	29	10	transported	transport	VBN
cord-104268-q1jx0n0l	29	11	to	to	IN
cord-104268-q1jx0n0l	29	12	the	the	DT
cord-104268-q1jx0n0l	29	13	cell	cell	NN
cord-104268-q1jx0n0l	29	14	surface	surface	NN
cord-104268-q1jx0n0l	29	15	through	through	IN
cord-104268-q1jx0n0l	29	16	a	a	DT
cord-104268-q1jx0n0l	29	17	bulk	bulk	JJ
cord-104268-q1jx0n0l	29	18	flow	flow	NN
cord-104268-q1jx0n0l	29	19	of	of	IN
cord-104268-q1jx0n0l	29	20	proteins	protein	NNS
cord-104268-q1jx0n0l	29	21	and	and	CC
cord-104268-q1jx0n0l	29	22	lipids	lipid	NNS
cord-104268-q1jx0n0l	29	23	(	(	-LRB-
cord-104268-q1jx0n0l	29	24	Pelham	Pelham	NNP
cord-104268-q1jx0n0l	29	25	and	and	CC
cord-104268-q1jx0n0l	29	26	Munro	Munro	NNP
cord-104268-q1jx0n0l	29	27	,	,	,
cord-104268-q1jx0n0l	29	28	1993	1993	CD
cord-104268-q1jx0n0l	29	29	)	)	-RRB-
cord-104268-q1jx0n0l	29	30	.	.	.
cord-104268-q1jx0n0l	30	1	In	in	IN
cord-104268-q1jx0n0l	30	2	yeast	yeast	NN
cord-104268-q1jx0n0l	30	3	,	,	,
cord-104268-q1jx0n0l	30	4	secretory	secretory	JJ
cord-104268-q1jx0n0l	30	5	pathway	pathway	NN
cord-104268-q1jx0n0l	30	6	membrane	membrane	NN
cord-104268-q1jx0n0l	30	7	glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	30	8	lacking	lack	VBG
cord-104268-q1jx0n0l	30	9	targeting	targeting	NN
cord-104268-q1jx0n0l	30	10	sequences	sequence	NNS
cord-104268-q1jx0n0l	30	11	are	be	VBP
cord-104268-q1jx0n0l	30	12	brought	bring	VBN
cord-104268-q1jx0n0l	30	13	by	by	IN
cord-104268-q1jx0n0l	30	14	default	default	NN
cord-104268-q1jx0n0l	30	15	to	to	IN
cord-104268-q1jx0n0l	30	16	the	the	DT
cord-104268-q1jx0n0l	30	17	vacuole	vacuole	NN
cord-104268-q1jx0n0l	30	18	(	(	-LRB-
cord-104268-q1jx0n0l	30	19	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	30	20	and	and	CC
cord-104268-q1jx0n0l	30	21	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	30	22	,	,	,
cord-104268-q1jx0n0l	30	23	1992	1992	CD
cord-104268-q1jx0n0l	30	24	;	;	:
cord-104268-q1jx0n0l	30	25	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	30	26	et	et	NNP
cord-104268-q1jx0n0l	30	27	al	al	NNP
cord-104268-q1jx0n0l	30	28	.	.	NNP
cord-104268-q1jx0n0l	30	29	,	,	,
cord-104268-q1jx0n0l	30	30	1992	1992	CD
cord-104268-q1jx0n0l	30	31	;	;	.
cord-104268-q1jx0n0l	31	1	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	31	2	et	et	NNP
cord-104268-q1jx0n0l	31	3	al	al	NNP
cord-104268-q1jx0n0l	31	4	.	.	.
cord-104268-q1jx0n0l	32	1	,	,	,
cord-104268-q1jx0n0l	32	2	1992	1992	CD
cord-104268-q1jx0n0l	32	3	;	;	:
cord-104268-q1jx0n0l	32	4	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	32	5	et	et	FW
cord-104268-q1jx0n0l	32	6	al	al	NNP
cord-104268-q1jx0n0l	32	7	.	.	.
cord-104268-q1jx0n0l	33	1	,	,	,
cord-104268-q1jx0n0l	33	2	1993	1993	CD
cord-104268-q1jx0n0l	33	3	;	;	:
cord-104268-q1jx0n0l	33	4	Gaynor	Gaynor	NNP
cord-104268-q1jx0n0l	33	5	et	et	NNP
cord-104268-q1jx0n0l	33	6	al	al	NNP
cord-104268-q1jx0n0l	33	7	.	.	.
cord-104268-q1jx0n0l	34	1	,	,	,
cord-104268-q1jx0n0l	34	2	1994	1994	CD
cord-104268-q1jx0n0l	34	3	;	;	:
cord-104268-q1jx0n0l	34	4	Hill	Hill	NNP
cord-104268-q1jx0n0l	34	5	and	and	CC
cord-104268-q1jx0n0l	34	6	Stevens	Stevens	NNP
cord-104268-q1jx0n0l	34	7	,	,	,
cord-104268-q1jx0n0l	34	8	1994	1994	CD
cord-104268-q1jx0n0l	34	9	)	)	-RRB-
cord-104268-q1jx0n0l	34	10	.	.	.
cord-104268-q1jx0n0l	35	1	Proteins	protein	NNS
cord-104268-q1jx0n0l	35	2	associated	associate	VBN
cord-104268-q1jx0n0l	35	3	with	with	IN
cord-104268-q1jx0n0l	35	4	glycan	glycan	NN
cord-104268-q1jx0n0l	35	5	modifications	modification	NNS
cord-104268-q1jx0n0l	35	6	constitute	constitute	VBP
cord-104268-q1jx0n0l	35	7	a	a	DT
cord-104268-q1jx0n0l	35	8	major	major	JJ
cord-104268-q1jx0n0l	35	9	class	class	NN
cord-104268-q1jx0n0l	35	10	of	of	IN
cord-104268-q1jx0n0l	35	11	resident	resident	JJ
cord-104268-q1jx0n0l	35	12	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	35	13	proteins	protein	NNS
cord-104268-q1jx0n0l	35	14	.	.	.
cord-104268-q1jx0n0l	36	1	Enzymes	enzyme	NNS
cord-104268-q1jx0n0l	36	2	belonging	belong	VBG
cord-104268-q1jx0n0l	36	3	to	to	IN
cord-104268-q1jx0n0l	36	4	different	different	JJ
cord-104268-q1jx0n0l	36	5	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	36	6	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	36	7	families	family	NNS
cord-104268-q1jx0n0l	36	8	share	share	VBP
cord-104268-q1jx0n0l	36	9	a	a	DT
cord-104268-q1jx0n0l	36	10	similar	similar	JJ
cord-104268-q1jx0n0l	36	11	type	type	NN
cord-104268-q1jx0n0l	36	12	II	ii	CD
cord-104268-q1jx0n0l	36	13	structural	structural	JJ
cord-104268-q1jx0n0l	36	14	organization	organization	NN
cord-104268-q1jx0n0l	36	15	,	,	,
cord-104268-q1jx0n0l	36	16	but	but	CC
cord-104268-q1jx0n0l	36	17	lack	lack	JJ
cord-104268-q1jx0n0l	36	18	amino	amino	NN
cord-104268-q1jx0n0l	36	19	acid	acid	NN
cord-104268-q1jx0n0l	36	20	sequence	sequence	NN
cord-104268-q1jx0n0l	36	21	homology	homology	NN
cord-104268-q1jx0n0l	36	22	or	or	CC
cord-104268-q1jx0n0l	36	23	discernible	discernible	JJ
cord-104268-q1jx0n0l	36	24	targeting	targeting	NN
cord-104268-q1jx0n0l	36	25	motifs	motif	NNS
cord-104268-q1jx0n0l	36	26	even	even	RB
cord-104268-q1jx0n0l	36	27	if	if	IN
cord-104268-q1jx0n0l	36	28	situated	situate	VBN
cord-104268-q1jx0n0l	36	29	in	in	IN
cord-104268-q1jx0n0l	36	30	the	the	DT
cord-104268-q1jx0n0l	36	31	same	same	JJ
cord-104268-q1jx0n0l	36	32	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	36	33	compartment	compartment	NN
cord-104268-q1jx0n0l	36	34	(	(	-LRB-
cord-104268-q1jx0n0l	36	35	Shaper	shaper	NN
cord-104268-q1jx0n0l	36	36	and	and	CC
cord-104268-q1jx0n0l	36	37	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	36	38	,	,	,
cord-104268-q1jx0n0l	36	39	1992	1992	CD
cord-104268-q1jx0n0l	36	40	;	;	:
cord-104268-q1jx0n0l	36	41	Kleene	Kleene	NNP
cord-104268-q1jx0n0l	36	42	and	and	CC
cord-104268-q1jx0n0l	36	43	Berger	Berger	NNP
cord-104268-q1jx0n0l	36	44	,	,	,
cord-104268-q1jx0n0l	36	45	1993	1993	CD
cord-104268-q1jx0n0l	36	46	)	)	-RRB-
cord-104268-q1jx0n0l	36	47	.	.	.
cord-104268-q1jx0n0l	37	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	37	2	has	have	VBZ
cord-104268-q1jx0n0l	37	3	been	be	VBN
cord-104268-q1jx0n0l	37	4	established	establish	VBN
cord-104268-q1jx0n0l	37	5	that	that	IN
cord-104268-q1jx0n0l	37	6	the	the	DT
cord-104268-q1jx0n0l	37	7	membrane	membrane	NN
cord-104268-q1jx0n0l	37	8	-	-	HYPH
cord-104268-q1jx0n0l	37	9	spanning	span	VBG
cord-104268-q1jx0n0l	37	10	domain	domain	NN
cord-104268-q1jx0n0l	37	11	of	of	IN
cord-104268-q1jx0n0l	37	12	animal	animal	NN
cord-104268-q1jx0n0l	37	13	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	37	14	plays	play	VBZ
cord-104268-q1jx0n0l	37	15	a	a	DT
cord-104268-q1jx0n0l	37	16	central	central	JJ
cord-104268-q1jx0n0l	37	17	role	role	NN
cord-104268-q1jx0n0l	37	18	in	in	IN
cord-104268-q1jx0n0l	37	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	37	20	localization	localization	NN
cord-104268-q1jx0n0l	37	21	(	(	-LRB-
cord-104268-q1jx0n0l	37	22	Munro	Munro	NNP
cord-104268-q1jx0n0l	37	23	,	,	,
cord-104268-q1jx0n0l	37	24	1991	1991	CD
cord-104268-q1jx0n0l	37	25	;	;	:
cord-104268-q1jx0n0l	37	26	Nilsson	Nilsson	NNP
cord-104268-q1jx0n0l	37	27	et	et	NNP
cord-104268-q1jx0n0l	37	28	al	al	NNP
cord-104268-q1jx0n0l	37	29	.	.	NNP
cord-104268-q1jx0n0l	37	30	,	,	,
cord-104268-q1jx0n0l	37	31	1991	1991	CD
cord-104268-q1jx0n0l	37	32	;	;	:
cord-104268-q1jx0n0l	37	33	Swift	Swift	NNP
cord-104268-q1jx0n0l	37	34	and	and	CC
cord-104268-q1jx0n0l	37	35	Machamer	Machamer	NNP
cord-104268-q1jx0n0l	37	36	,	,	,
cord-104268-q1jx0n0l	37	37	1991	1991	CD
cord-104268-q1jx0n0l	37	38	;	;	:
cord-104268-q1jx0n0l	37	39	Aoki	Aoki	NNP
cord-104268-q1jx0n0l	37	40	et	et	NNP
cord-104268-q1jx0n0l	37	41	al	al	NNP
cord-104268-q1jx0n0l	37	42	.	.	NNP
cord-104268-q1jx0n0l	37	43	,	,	,
cord-104268-q1jx0n0l	37	44	1992	1992	CD
cord-104268-q1jx0n0l	37	45	;	;	:
cord-104268-q1jx0n0l	37	46	Burke	Burke	NNP
cord-104268-q1jx0n0l	37	47	et	et	NNP
cord-104268-q1jx0n0l	37	48	al	al	NNP
cord-104268-q1jx0n0l	37	49	.	.	.
cord-104268-q1jx0n0l	38	1	,	,	,
cord-104268-q1jx0n0l	38	2	1992	1992	CD
cord-104268-q1jx0n0l	38	3	;	;	:
cord-104268-q1jx0n0l	38	4	Colley	Colley	NNP
cord-104268-q1jx0n0l	38	5	et	et	NNP
cord-104268-q1jx0n0l	38	6	al	al	NNP
cord-104268-q1jx0n0l	38	7	.	.	NNP
cord-104268-q1jx0n0l	38	8	,	,	,
cord-104268-q1jx0n0l	38	9	1992	1992	CD
cord-104268-q1jx0n0l	38	10	;	;	:
cord-104268-q1jx0n0l	38	11	Tang	Tang	NNP
cord-104268-q1jx0n0l	38	12	et	et	FW
cord-104268-q1jx0n0l	38	13	al	al	NNP
cord-104268-q1jx0n0l	38	14	.	.	.
cord-104268-q1jx0n0l	39	1	,	,	,
cord-104268-q1jx0n0l	39	2	1992	1992	CD
cord-104268-q1jx0n0l	39	3	;	;	:
cord-104268-q1jx0n0l	39	4	Teasdale	Teasdale	NNP
cord-104268-q1jx0n0l	39	5	et	et	NNP
cord-104268-q1jx0n0l	39	6	al	al	NNP
cord-104268-q1jx0n0l	39	7	.	.	.
cord-104268-q1jx0n0l	40	1	,	,	,
cord-104268-q1jx0n0l	40	2	1992	1992	CD
cord-104268-q1jx0n0l	40	3	;	;	:
cord-104268-q1jx0n0l	40	4	Wong	Wong	NNP
cord-104268-q1jx0n0l	40	5	et	et	NNP
cord-104268-q1jx0n0l	40	6	al	al	NNP
cord-104268-q1jx0n0l	40	7	.	.	NNP
cord-104268-q1jx0n0l	40	8	,	,	,
cord-104268-q1jx0n0l	40	9	1992	1992	CD
cord-104268-q1jx0n0l	40	10	;	;	:
cord-104268-q1jx0n0l	40	11	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	40	12	et	et	NNP
cord-104268-q1jx0n0l	40	13	al	al	NNP
cord-104268-q1jx0n0l	40	14	.	.	.
cord-104268-q1jx0n0l	41	1	,	,	,
cord-104268-q1jx0n0l	41	2	1994	1994	CD
cord-104268-q1jx0n0l	41	3	;	;	:
cord-104268-q1jx0n0l	41	4	Low	Low	NNP
cord-104268-q1jx0n0l	41	5	and	and	CC
cord-104268-q1jx0n0l	41	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	41	7	,	,	,
cord-104268-q1jx0n0l	41	8	i994	i994	NN
cord-104268-q1jx0n0l	41	9	)	)	-RRB-
cord-104268-q1jx0n0l	41	10	.	.	.
cord-104268-q1jx0n0l	42	1	To	to	TO
cord-104268-q1jx0n0l	42	2	define	define	VB
cord-104268-q1jx0n0l	42	3	more	more	RBR
cord-104268-q1jx0n0l	42	4	precisely	precisely	RB
cord-104268-q1jx0n0l	42	5	the	the	DT
cord-104268-q1jx0n0l	42	6	S.	S.	NNP
cord-104268-q1jx0n0l	42	7	cerevisiae	cerevisiae	NN
cord-104268-q1jx0n0l	42	8	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	42	9	complex	complex	NN
cord-104268-q1jx0n0l	42	10	and	and	CC
cord-104268-q1jx0n0l	42	11	to	to	TO
cord-104268-q1jx0n0l	42	12	better	better	RB
cord-104268-q1jx0n0l	42	13	understand	understand	VB
cord-104268-q1jx0n0l	42	14	how	how	WRB
cord-104268-q1jx0n0l	42	15	posttranslational	posttranslational	JJ
cord-104268-q1jx0n0l	42	16	modifications	modification	NNS
cord-104268-q1jx0n0l	42	17	occur	occur	VBP
cord-104268-q1jx0n0l	42	18	,	,	,
cord-104268-q1jx0n0l	42	19	we	-PRON-	PRP
cord-104268-q1jx0n0l	42	20	have	have	VBP
cord-104268-q1jx0n0l	42	21	studied	study	VBN
cord-104268-q1jx0n0l	42	22	the	the	DT
cord-104268-q1jx0n0l	42	23	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	42	24	,	,	,
cord-104268-q1jx0n0l	42	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	42	26	/	/	SYM
cord-104268-q1jx0n0l	42	27	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	42	28	.	.	.
cord-104268-q1jx0n0l	43	1	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	43	2	/	/	SYM
cord-104268-q1jx0n0l	43	3	MNT1	MNT1	NNP
cord-104268-q1jx0n0l	43	4	was	be	VBD
cord-104268-q1jx0n0l	43	5	isolated	isolate	VBN
cord-104268-q1jx0n0l	43	6	as	as	IN
cord-104268-q1jx0n0l	43	7	a	a	DT
cord-104268-q1jx0n0l	43	8	gene	gene	NN
cord-104268-q1jx0n0l	43	9	implicated	implicate	VBN
cord-104268-q1jx0n0l	43	10	in	in	IN
cord-104268-q1jx0n0l	43	11	cell	cell	NN
cord-104268-q1jx0n0l	43	12	-	-	HYPH
cord-104268-q1jx0n0l	43	13	wall	wall	NN
cord-104268-q1jx0n0l	43	14	assembly	assembly	NN
cord-104268-q1jx0n0l	43	15	conferring	confer	VBG
cord-104268-q1jx0n0l	43	16	K1	K1	NNP
cord-104268-q1jx0n0l	43	17	killer	killer	NN
cord-104268-q1jx0n0l	43	18	toxin	toxin	NN
cord-104268-q1jx0n0l	43	19	resistance	resistance	NN
cord-104268-q1jx0n0l	43	20	when	when	WRB
cord-104268-q1jx0n0l	43	21	mutated	mutate	VBN
cord-104268-q1jx0n0l	43	22	(	(	-LRB-
cord-104268-q1jx0n0l	43	23	Hill	Hill	NNP
cord-104268-q1jx0n0l	43	24	et	et	NNP
cord-104268-q1jx0n0l	43	25	al	al	NNP
cord-104268-q1jx0n0l	43	26	.	.	NNP
cord-104268-q1jx0n0l	43	27	,	,	,
cord-104268-q1jx0n0l	43	28	1992	1992	CD
cord-104268-q1jx0n0l	43	29	)	)	-RRB-
cord-104268-q1jx0n0l	43	30	and	and	CC
cord-104268-q1jx0n0l	43	31	found	find	VBD
cord-104268-q1jx0n0l	43	32	to	to	TO
cord-104268-q1jx0n0l	43	33	encode	encode	VB
cord-104268-q1jx0n0l	43	34	an	an	DT
cord-104268-q1jx0n0l	43	35	cd,2-mannosyltransferase	cd,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	43	36	(	(	-LRB-
cord-104268-q1jx0n0l	43	37	H	h	NN
cord-104268-q1jx0n0l	43	38	/	/	SYM
cord-104268-q1jx0n0l	43	39	iusler	iusler	NN
cord-104268-q1jx0n0l	43	40	and	and	CC
cord-104268-q1jx0n0l	43	41	.	.	.
cord-104268-q1jx0n0l	44	1	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	44	2	/	/	SYM
cord-104268-q1jx0n0l	44	3	MNT1	MNT1	NNP
cord-104268-q1jx0n0l	44	4	encodes	encode	VBZ
cord-104268-q1jx0n0l	44	5	a	a	DT
cord-104268-q1jx0n0l	44	6	442-amino	442-amino	CD
cord-104268-q1jx0n0l	44	7	acid	acid	NN
cord-104268-q1jx0n0l	44	8	residue	residue	NN
cord-104268-q1jx0n0l	44	9	predicted	predict	VBD
cord-104268-q1jx0n0l	44	10	type	type	NN
cord-104268-q1jx0n0l	44	11	II	II	NNP
cord-104268-q1jx0n0l	44	12	membrane	membrane	NN
cord-104268-q1jx0n0l	44	13	protein	protein	NN
cord-104268-q1jx0n0l	44	14	containing	contain	VBG
cord-104268-q1jx0n0l	44	15	a	a	DT
cord-104268-q1jx0n0l	44	16	putative	putative	JJ
cord-104268-q1jx0n0l	44	17	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	44	18	domain	domain	NN
cord-104268-q1jx0n0l	44	19	near	near	IN
cord-104268-q1jx0n0l	44	20	its	-PRON-	PRP$
cord-104268-q1jx0n0l	44	21	NH2	NH2	NNP
cord-104268-q1jx0n0l	44	22	terminus	terminus	NN
cord-104268-q1jx0n0l	44	23	and	and	CC
cord-104268-q1jx0n0l	44	24	one	one	CD
cord-104268-q1jx0n0l	44	25	potential	potential	JJ
cord-104268-q1jx0n0l	44	26	luminal	luminal	JJ
cord-104268-q1jx0n0l	44	27	N	n	NN
cord-104268-q1jx0n0l	44	28	-	-	HYPH
cord-104268-q1jx0n0l	44	29	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	44	30	site	site	NN
cord-104268-q1jx0n0l	44	31	.	.	.
cord-104268-q1jx0n0l	45	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	45	2	/	/	SYM
cord-104268-q1jx0n0l	45	3	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	45	4	specifically	specifically	RB
cord-104268-q1jx0n0l	45	5	adds	add	VBZ
cord-104268-q1jx0n0l	45	6	a	a	DT
cord-104268-q1jx0n0l	45	7	third	third	JJ
cord-104268-q1jx0n0l	45	8	mannose	mannose	NN
cord-104268-q1jx0n0l	45	9	during	during	IN
cord-104268-q1jx0n0l	45	10	the	the	DT
cord-104268-q1jx0n0l	45	11	linear	linear	JJ
cord-104268-q1jx0n0l	45	12	elongation	elongation	NN
cord-104268-q1jx0n0l	45	13	of	of	IN
cord-104268-q1jx0n0l	45	14	O	o	NN
cord-104268-q1jx0n0l	45	15	-	-	HYPH
cord-104268-q1jx0n0l	45	16	linked	link	VBN
cord-104268-q1jx0n0l	45	17	carbohydrate	carbohydrate	NN
cord-104268-q1jx0n0l	45	18	chains	chain	NNS
cord-104268-q1jx0n0l	45	19	that	that	WDT
cord-104268-q1jx0n0l	45	20	may	may	MD
cord-104268-q1jx0n0l	45	21	contain	contain	VB
cord-104268-q1jx0n0l	45	22	up	up	IN
cord-104268-q1jx0n0l	45	23	to	to	TO
cord-104268-q1jx0n0l	45	24	five	five	CD
cord-104268-q1jx0n0l	45	25	mannose	mannose	NN
cord-104268-q1jx0n0l	45	26	residues	residue	NNS
cord-104268-q1jx0n0l	45	27	(	(	-LRB-
cord-104268-q1jx0n0l	45	28	H	h	NN
cord-104268-q1jx0n0l	45	29	~	~	NFP
cord-104268-q1jx0n0l	45	30	usler	usler	NNP
cord-104268-q1jx0n0l	45	31	et	et	NNP
cord-104268-q1jx0n0l	45	32	al	al	NNP
cord-104268-q1jx0n0l	45	33	.	.	NNP
cord-104268-q1jx0n0l	45	34	,	,	,
cord-104268-q1jx0n0l	45	35	1992	1992	CD
cord-104268-q1jx0n0l	45	36	)	)	-RRB-
cord-104268-q1jx0n0l	45	37	and	and	CC
cord-104268-q1jx0n0l	45	38	is	be	VBZ
cord-104268-q1jx0n0l	45	39	also	also	RB
cord-104268-q1jx0n0l	45	40	apparently	apparently	RB
cord-104268-q1jx0n0l	45	41	involved	involve	VBN
cord-104268-q1jx0n0l	45	42	in	in	IN
cord-104268-q1jx0n0l	45	43	asparagine	asparagine	NN
cord-104268-q1jx0n0l	45	44	-	-	HYPH
cord-104268-q1jx0n0l	45	45	linked	link	VBN
cord-104268-q1jx0n0l	45	46	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	45	47	(	(	-LRB-
cord-104268-q1jx0n0l	45	48	Hill	Hill	NNP
cord-104268-q1jx0n0l	45	49	et	et	FW
cord-104268-q1jx0n0l	45	50	al	al	NNP
cord-104268-q1jx0n0l	45	51	.	.	.
cord-104268-q1jx0n0l	46	1	,	,	,
cord-104268-q1jx0n0l	46	2	1992	1992	CD
cord-104268-q1jx0n0l	46	3	;	;	:
cord-104268-q1jx0n0l	46	4	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	46	5	et	et	NNP
cord-104268-q1jx0n0l	46	6	al	al	NNP
cord-104268-q1jx0n0l	46	7	.	.	NNP
cord-104268-q1jx0n0l	46	8	,	,	,
cord-104268-q1jx0n0l	46	9	1995b	1995b	CD
cord-104268-q1jx0n0l	46	10	)	)	-RRB-
cord-104268-q1jx0n0l	46	11	.	.	.
cord-104268-q1jx0n0l	47	1	Here	here	RB
cord-104268-q1jx0n0l	47	2	we	-PRON-	PRP
cord-104268-q1jx0n0l	47	3	report	report	VBP
cord-104268-q1jx0n0l	47	4	further	further	JJ
cord-104268-q1jx0n0l	47	5	characterization	characterization	NN
cord-104268-q1jx0n0l	47	6	of	of	IN
cord-104268-q1jx0n0l	47	7	the	the	DT
cord-104268-q1jx0n0l	47	8	KRE2/	KRE2/	NNP
cord-104268-q1jx0n0l	47	9	MNTl	MNTl	NNS
cord-104268-q1jx0n0l	47	10	-	-	HYPH
cord-104268-q1jx0n0l	47	11	encoded	encode	VBN
cord-104268-q1jx0n0l	47	12	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	47	13	.	.	.
cord-104268-q1jx0n0l	48	1	We	-PRON-	PRP
cord-104268-q1jx0n0l	48	2	demonstrate	demonstrate	VBP
cord-104268-q1jx0n0l	48	3	that	that	IN
cord-104268-q1jx0n0l	48	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	48	5	/	/	SYM
cord-104268-q1jx0n0l	48	6	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	48	7	possesses	possess	VBZ
cord-104268-q1jx0n0l	48	8	a	a	DT
cord-104268-q1jx0n0l	48	9	type	type	NN
cord-104268-q1jx0n0l	48	10	II	II	NNP
cord-104268-q1jx0n0l	48	11	orientation	orientation	NN
cord-104268-q1jx0n0l	48	12	,	,	,
cord-104268-q1jx0n0l	48	13	is	be	VBZ
cord-104268-q1jx0n0l	48	14	progressively	progressively	RB
cord-104268-q1jx0n0l	48	15	N	n	CD
cord-104268-q1jx0n0l	48	16	-	-	HYPH
cord-104268-q1jx0n0l	48	17	glycosylated	glycosylate	VBN
cord-104268-q1jx0n0l	48	18	in	in	IN
cord-104268-q1jx0n0l	48	19	an	an	DT
cord-104268-q1jx0n0l	48	20	MNNl	MNNl	NNS
cord-104268-q1jx0n0l	48	21	-	-	HYPH
cord-104268-q1jx0n0l	48	22	dependent	dependent	JJ
cord-104268-q1jx0n0l	48	23	fashion	fashion	NN
cord-104268-q1jx0n0l	48	24	,	,	,
cord-104268-q1jx0n0l	48	25	and	and	CC
cord-104268-q1jx0n0l	48	26	is	be	VBZ
cord-104268-q1jx0n0l	48	27	localized	localize	VBN
cord-104268-q1jx0n0l	48	28	in	in	IN
cord-104268-q1jx0n0l	48	29	a	a	DT
cord-104268-q1jx0n0l	48	30	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	48	31	subcompartment	subcompartment	NN
cord-104268-q1jx0n0l	48	32	that	that	WDT
cord-104268-q1jx0n0l	48	33	overlaps	overlap	VBZ
cord-104268-q1jx0n0l	48	34	with	with	IN
cord-104268-q1jx0n0l	48	35	the	the	DT
cord-104268-q1jx0n0l	48	36	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	48	37	medial	medial	JJ
cord-104268-q1jx0n0l	48	38	-	-	HYPH
cord-104268-q1jx0n0l	48	39	Golgi	golgi	NN
cord-104268-q1jx0n0l	48	40	compartment	compartment	NN
cord-104268-q1jx0n0l	48	41	.	.	.
cord-104268-q1jx0n0l	49	1	In	in	IN
cord-104268-q1jx0n0l	49	2	addition	addition	NN
cord-104268-q1jx0n0l	49	3	,	,	,
cord-104268-q1jx0n0l	49	4	we	-PRON-	PRP
cord-104268-q1jx0n0l	49	5	show	show	VBP
cord-104268-q1jx0n0l	49	6	that	that	IN
cord-104268-q1jx0n0l	49	7	the	the	DT
cord-104268-q1jx0n0l	49	8	short	short	JJ
cord-104268-q1jx0n0l	49	9	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	49	10	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	49	11	tail	tail	NN
cord-104268-q1jx0n0l	49	12	domain	domain	NN
cord-104268-q1jx0n0l	49	13	of	of	IN
cord-104268-q1jx0n0l	49	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	49	15	/	/	SYM
cord-104268-q1jx0n0l	49	16	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	49	17	is	be	VBZ
cord-104268-q1jx0n0l	49	18	required	require	VBN
cord-104268-q1jx0n0l	49	19	for	for	IN
cord-104268-q1jx0n0l	49	20	correct	correct	JJ
cord-104268-q1jx0n0l	49	21	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	49	22	localization	localization	NN
cord-104268-q1jx0n0l	49	23	,	,	,
cord-104268-q1jx0n0l	49	24	whereas	whereas	IN
cord-104268-q1jx0n0l	49	25	the	the	DT
cord-104268-q1jx0n0l	49	26	membrane	membrane	NN
cord-104268-q1jx0n0l	49	27	-	-	HYPH
cord-104268-q1jx0n0l	49	28	spanning	span	VBG
cord-104268-q1jx0n0l	49	29	and	and	CC
cord-104268-q1jx0n0l	49	30	stem	stem	NN
cord-104268-q1jx0n0l	49	31	domains	domain	NNS
cord-104268-q1jx0n0l	49	32	are	be	VBP
cord-104268-q1jx0n0l	49	33	dispensable	dispensable	JJ
cord-104268-q1jx0n0l	49	34	.	.	.
cord-104268-q1jx0n0l	50	1	However	however	RB
cord-104268-q1jx0n0l	50	2	,	,	,
cord-104268-q1jx0n0l	50	3	localization	localization	NN
cord-104268-q1jx0n0l	50	4	of	of	IN
cord-104268-q1jx0n0l	50	5	a	a	DT
cord-104268-q1jx0n0l	50	6	reporter	reporter	NN
cord-104268-q1jx0n0l	50	7	protein	protein	NN
cord-104268-q1jx0n0l	50	8	to	to	IN
cord-104268-q1jx0n0l	50	9	the	the	DT
cord-104268-q1jx0n0l	50	10	yeast	yeast	NN
cord-104268-q1jx0n0l	50	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	50	12	requires	require	VBZ
cord-104268-q1jx0n0l	50	13	a	a	DT
cord-104268-q1jx0n0l	50	14	region	region	NN
cord-104268-q1jx0n0l	50	15	of	of	IN
cord-104268-q1jx0n0l	50	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	50	17	/	/	SYM
cord-104268-q1jx0n0l	50	18	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	50	19	encompassing	encompass	VBG
cord-104268-q1jx0n0l	50	20	the	the	DT
cord-104268-q1jx0n0l	50	21	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	50	22	tail	tail	NN
cord-104268-q1jx0n0l	50	23	,	,	,
cord-104268-q1jx0n0l	50	24	the	the	DT
cord-104268-q1jx0n0l	50	25	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	50	26	domain	domain	NN
cord-104268-q1jx0n0l	50	27	(	(	-LRB-
cord-104268-q1jx0n0l	50	28	TMD	TMD	NNP
cord-104268-q1jx0n0l	50	29	)	)	-RRB-
cord-104268-q1jx0n0l	50	30	,	,	,
cord-104268-q1jx0n0l	50	31	and	and	CC
cord-104268-q1jx0n0l	50	32	a	a	DT
cord-104268-q1jx0n0l	50	33	segment	segment	NN
cord-104268-q1jx0n0l	50	34	of	of	IN
cord-104268-q1jx0n0l	50	35	the	the	DT
cord-104268-q1jx0n0l	50	36	luminal	luminal	JJ
cord-104268-q1jx0n0l	50	37	stem	stem	NN
cord-104268-q1jx0n0l	50	38	domain	domain	NN
cord-104268-q1jx0n0l	50	39	.	.	.
cord-104268-q1jx0n0l	51	1	All	all	DT
cord-104268-q1jx0n0l	51	2	yeast	yeast	NN
cord-104268-q1jx0n0l	51	3	constructions	construction	NNS
cord-104268-q1jx0n0l	51	4	used	use	VBD
cord-104268-q1jx0n0l	51	5	strains	strain	NNS
cord-104268-q1jx0n0l	51	6	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	51	7	(	(	-LRB-
cord-104268-q1jx0n0l	51	8	MATa	MATa	NNS
cord-104268-q1jx0n0l	51	9	,	,	,
cord-104268-q1jx0n0l	51	10	(	(	-LRB-
cord-104268-q1jx0n0l	51	11	Boone	Boone	NNP
cord-104268-q1jx0n0l	51	12	et	et	NNP
cord-104268-q1jx0n0l	51	13	al	al	NNP
cord-104268-q1jx0n0l	51	14	.	.	NNP
cord-104268-q1jx0n0l	51	15	,	,	,
cord-104268-q1jx0n0l	51	16	1990	1990	CD
cord-104268-q1jx0n0l	51	17	)	)	-RRB-
cord-104268-q1jx0n0l	51	18	.	.	.
cord-104268-q1jx0n0l	52	1	Strains	strain	NNS
cord-104268-q1jx0n0l	52	2	were	be	VBD
cord-104268-q1jx0n0l	52	3	transformed	transform	VBN
cord-104268-q1jx0n0l	52	4	using	use	VBG
cord-104268-q1jx0n0l	52	5	the	the	DT
cord-104268-q1jx0n0l	52	6	lithium	lithium	NN
cord-104268-q1jx0n0l	52	7	acetate	acetate	NN
cord-104268-q1jx0n0l	52	8	procedure	procedure	NN
cord-104268-q1jx0n0l	52	9	using	use	VBG
cord-104268-q1jx0n0l	52	10	sheared	shear	VBN
cord-104268-q1jx0n0l	52	11	,	,	,
cord-104268-q1jx0n0l	52	12	denatured	denatured	JJ
cord-104268-q1jx0n0l	52	13	carrier	carrier	NN
cord-104268-q1jx0n0l	52	14	DNA	dna	NN
cord-104268-q1jx0n0l	52	15	(	(	-LRB-
cord-104268-q1jx0n0l	52	16	Gietz	Gietz	NNP
cord-104268-q1jx0n0l	52	17	et	et	NNP
cord-104268-q1jx0n0l	52	18	al	al	NNP
cord-104268-q1jx0n0l	52	19	.	.	NNP
cord-104268-q1jx0n0l	52	20	,	,	,
cord-104268-q1jx0n0l	52	21	1995	1995	CD
cord-104268-q1jx0n0l	52	22	)	)	-RRB-
cord-104268-q1jx0n0l	52	23	.	.	.
cord-104268-q1jx0n0l	53	1	Transformants	transformant	NNS
cord-104268-q1jx0n0l	53	2	were	be	VBD
cord-104268-q1jx0n0l	53	3	selected	select	VBN
cord-104268-q1jx0n0l	53	4	on	on	IN
cord-104268-q1jx0n0l	53	5	synthetic	synthetic	JJ
cord-104268-q1jx0n0l	53	6	minimal	minimal	JJ
cord-104268-q1jx0n0l	53	7	medium	medium	NN
cord-104268-q1jx0n0l	53	8	with	with	IN
cord-104268-q1jx0n0l	53	9	auxotrophic	auxotrophic	JJ
cord-104268-q1jx0n0l	53	10	supplements	supplement	NNS
cord-104268-q1jx0n0l	53	11	.	.	.
cord-104268-q1jx0n0l	54	1	Levels	level	NNS
cord-104268-q1jx0n0l	54	2	of	of	IN
cord-104268-q1jx0n0l	54	3	sensitivity	sensitivity	NN
cord-104268-q1jx0n0l	54	4	to	to	IN
cord-104268-q1jx0n0l	54	5	K1	K1	NNP
cord-104268-q1jx0n0l	54	6	killer	killer	NN
cord-104268-q1jx0n0l	54	7	toxin	toxin	NN
cord-104268-q1jx0n0l	54	8	were	be	VBD
cord-104268-q1jx0n0l	54	9	evaluated	evaluate	VBN
cord-104268-q1jx0n0l	54	10	in	in	IN
cord-104268-q1jx0n0l	54	11	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	54	12	by	by	IN
cord-104268-q1jx0n0l	54	13	a	a	DT
cord-104268-q1jx0n0l	54	14	seeded	seed	VBN
cord-104268-q1jx0n0l	54	15	plate	plate	NN
cord-104268-q1jx0n0l	54	16	assay	assay	NN
cord-104268-q1jx0n0l	54	17	using	use	VBG
cord-104268-q1jx0n0l	54	18	a	a	DT
cord-104268-q1jx0n0l	54	19	modified	modify	VBN
cord-104268-q1jx0n0l	54	20	medium	medium	NN
cord-104268-q1jx0n0l	54	21	consisting	consist	VBG
cord-104268-q1jx0n0l	54	22	of	of	IN
cord-104268-q1jx0n0l	54	23	0.67	0.67	CD
cord-104268-q1jx0n0l	54	24	%	%	NN
cord-104268-q1jx0n0l	54	25	YNB	YNB	NNP
cord-104268-q1jx0n0l	54	26	,	,	,
cord-104268-q1jx0n0l	54	27	0.0025	0.0025	CD
cord-104268-q1jx0n0l	54	28	%	%	NN
cord-104268-q1jx0n0l	54	29	required	require	VBN
cord-104268-q1jx0n0l	54	30	amino	amino	NN
cord-104268-q1jx0n0l	54	31	acids	acid	NNS
cord-104268-q1jx0n0l	54	32	,	,	,
cord-104268-q1jx0n0l	54	33	1.0	1.0	CD
cord-104268-q1jx0n0l	54	34	%	%	NN
cord-104268-q1jx0n0l	54	35	bacto	bacto	NNP
cord-104268-q1jx0n0l	54	36	agar	agar	NNP
cord-104268-q1jx0n0l	54	37	,	,	,
cord-104268-q1jx0n0l	54	38	0.001	0.001	CD
cord-104268-q1jx0n0l	54	39	%	%	NN
cord-104268-q1jx0n0l	54	40	methylene	methylene	NN
cord-104268-q1jx0n0l	54	41	blue	blue	JJ
cord-104268-q1jx0n0l	54	42	,	,	,
cord-104268-q1jx0n0l	54	43	2	2	CD
cord-104268-q1jx0n0l	54	44	%	%	NN
cord-104268-q1jx0n0l	54	45	glucose	glucose	NN
cord-104268-q1jx0n0l	54	46	,	,	,
cord-104268-q1jx0n0l	54	47	and	and	CC
cord-104268-q1jx0n0l	54	48	buffered	buffer	VBN
cord-104268-q1jx0n0l	54	49	to	to	IN
cord-104268-q1jx0n0l	54	50	pH	pH	NNP
cord-104268-q1jx0n0l	54	51	4.7	4.7	CD
cord-104268-q1jx0n0l	54	52	with	with	IN
cord-104268-q1jx0n0l	54	53	Halvorson	Halvorson	NNP
cord-104268-q1jx0n0l	54	54	minimal	minimal	JJ
cord-104268-q1jx0n0l	54	55	medium	medium	NN
cord-104268-q1jx0n0l	55	1	(	(	-LRB-
cord-104268-q1jx0n0l	55	2	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	55	3	et	et	NNP
cord-104268-q1jx0n0l	55	4	al	al	NNP
cord-104268-q1jx0n0l	55	5	.	.	NNP
cord-104268-q1jx0n0l	55	6	,	,	,
cord-104268-q1jx0n0l	55	7	1993	1993	CD
cord-104268-q1jx0n0l	56	1	(	(	-LRB-
cord-104268-q1jx0n0l	56	2	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	56	3	et	et	NNP
cord-104268-q1jx0n0l	56	4	al	al	NNP
cord-104268-q1jx0n0l	56	5	.	.	.
cord-104268-q1jx0n0l	57	1	,	,	,
cord-104268-q1jx0n0l	57	2	,	,	,
cord-104268-q1jx0n0l	57	3	1995a	1995a	CD
cord-104268-q1jx0n0l	57	4	Brown	Brown	NNP
cord-104268-q1jx0n0l	57	5	et	et	NNP
cord-104268-q1jx0n0l	57	6	al	al	NNP
cord-104268-q1jx0n0l	57	7	.	.	NNP
cord-104268-q1jx0n0l	57	8	,	,	,
cord-104268-q1jx0n0l	57	9	1994	1994	CD
cord-104268-q1jx0n0l	57	10	)	)	-RRB-
cord-104268-q1jx0n0l	57	11	.	.	.
cord-104268-q1jx0n0l	58	1	(	(	-LRB-
cord-104268-q1jx0n0l	58	2	underlined	underline	VBN
cord-104268-q1jx0n0l	58	3	)	)	-RRB-
cord-104268-q1jx0n0l	58	4	.	.	.
cord-104268-q1jx0n0l	59	1	Finally	finally	RB
cord-104268-q1jx0n0l	59	2	,	,	,
cord-104268-q1jx0n0l	59	3	KPKP	KPKP	NNP
cord-104268-q1jx0n0l	59	4	was	be	VBD
cord-104268-q1jx0n0l	59	5	obtained	obtain	VBN
cord-104268-q1jx0n0l	59	6	by	by	IN
cord-104268-q1jx0n0l	59	7	replacing	replace	VBG
cord-104268-q1jx0n0l	59	8	the	the	DT
cord-104268-q1jx0n0l	59	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	59	10	TMD	TMD	NNP
cord-104268-q1jx0n0l	59	11	in	in	IN
cord-104268-q1jx0n0l	59	12	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	59	13	with	with	IN
cord-104268-q1jx0n0l	59	14	that	that	DT
cord-104268-q1jx0n0l	59	15	of	of	IN
cord-104268-q1jx0n0l	59	16	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	59	17	using	use	VBG
cord-104268-q1jx0n0l	59	18	the	the	DT
cord-104268-q1jx0n0l	59	19	same	same	JJ
cord-104268-q1jx0n0l	59	20	oligonucleotide	oligonucleotide	NN
cord-104268-q1jx0n0l	59	21	that	that	WDT
cord-104268-q1jx0n0l	59	22	was	be	VBD
cord-104268-q1jx0n0l	59	23	used	use	VBN
cord-104268-q1jx0n0l	59	24	to	to	TO
cord-104268-q1jx0n0l	59	25	obtain	obtain	VB
cord-104268-q1jx0n0l	59	26	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	60	1	All	all	DT
cord-104268-q1jx0n0l	60	2	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	60	3	protein	protein	NN
cord-104268-q1jx0n0l	60	4	constructions	construction	NNS
cord-104268-q1jx0n0l	60	5	were	be	VBD
cord-104268-q1jx0n0l	60	6	verified	verify	VBN
cord-104268-q1jx0n0l	60	7	by	by	IN
cord-104268-q1jx0n0l	60	8	DNA	dna	NN
cord-104268-q1jx0n0l	60	9	sequencing	sequencing	NN
cord-104268-q1jx0n0l	60	10	using	use	VBG
cord-104268-q1jx0n0l	60	11	the	the	DT
cord-104268-q1jx0n0l	60	12	dideoxy	dideoxy	NN
cord-104268-q1jx0n0l	60	13	chain	chain	NN
cord-104268-q1jx0n0l	60	14	termination	termination	NN
cord-104268-q1jx0n0l	60	15	procedure	procedure	NN
cord-104268-q1jx0n0l	60	16	(	(	-LRB-
cord-104268-q1jx0n0l	60	17	Sanger	Sanger	NNP
cord-104268-q1jx0n0l	60	18	et	et	NNP
cord-104268-q1jx0n0l	60	19	al	al	NNP
cord-104268-q1jx0n0l	60	20	.	.	NNP
cord-104268-q1jx0n0l	60	21	,	,	,
cord-104268-q1jx0n0l	60	22	1977	1977	CD
cord-104268-q1jx0n0l	60	23	)	)	-RRB-
cord-104268-q1jx0n0l	60	24	with	with	IN
cord-104268-q1jx0n0l	60	25	the	the	DT
cord-104268-q1jx0n0l	60	26	Sequenase	Sequenase	NNP
cord-104268-q1jx0n0l	60	27	enzyme	enzyme	NN
cord-104268-q1jx0n0l	60	28	(	(	-LRB-
cord-104268-q1jx0n0l	60	29	United	United	NNP
cord-104268-q1jx0n0l	60	30	States	States	NNP
cord-104268-q1jx0n0l	60	31	Biochemical	Biochemical	NNP
cord-104268-q1jx0n0l	60	32	Corp.	Corp.	NNP
cord-104268-q1jx0n0l	60	33	,	,	,
cord-104268-q1jx0n0l	60	34	Cleveland	Cleveland	NNP
cord-104268-q1jx0n0l	60	35	,	,	,
cord-104268-q1jx0n0l	60	36	OH	OH	NNP
cord-104268-q1jx0n0l	60	37	)	)	-RRB-
cord-104268-q1jx0n0l	60	38	,	,	,
cord-104268-q1jx0n0l	60	39	a	a	DT
cord-104268-q1jx0n0l	60	40	-	-	HYPH
cord-104268-q1jx0n0l	60	41	asS	as	NNS
cord-104268-q1jx0n0l	60	42	-	-	HYPH
cord-104268-q1jx0n0l	60	43	dATP	datp	NN
cord-104268-q1jx0n0l	60	44	and	and	CC
cord-104268-q1jx0n0l	60	45	specific	specific	JJ
cord-104268-q1jx0n0l	60	46	DNA	dna	NN
cord-104268-q1jx0n0l	60	47	primers	primer	NNS
cord-104268-q1jx0n0l	60	48	.	.	.
cord-104268-q1jx0n0l	61	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	61	2	antibodies	antibody	NNS
cord-104268-q1jx0n0l	61	3	were	be	VBD
cord-104268-q1jx0n0l	61	4	raised	raise	VBN
cord-104268-q1jx0n0l	61	5	in	in	IN
cord-104268-q1jx0n0l	61	6	rabbits	rabbit	NNS
cord-104268-q1jx0n0l	61	7	against	against	IN
cord-104268-q1jx0n0l	61	8	a	a	DT
cord-104268-q1jx0n0l	61	9	BSA	BSA	NNP
cord-104268-q1jx0n0l	61	10	-	-	HYPH
cord-104268-q1jx0n0l	61	11	coupled	couple	VBN
cord-104268-q1jx0n0l	61	12	synthetic	synthetic	JJ
cord-104268-q1jx0n0l	61	13	peptide	peptide	NN
cord-104268-q1jx0n0l	61	14	corresponding	correspond	VBG
cord-104268-q1jx0n0l	61	15	to	to	IN
cord-104268-q1jx0n0l	61	16	the	the	DT
cord-104268-q1jx0n0l	61	17	last	last	JJ
cord-104268-q1jx0n0l	61	18	10	10	CD
cord-104268-q1jx0n0l	61	19	amino	amino	NN
cord-104268-q1jx0n0l	61	20	acid	acid	NN
cord-104268-q1jx0n0l	61	21	residues	residue	NNS
cord-104268-q1jx0n0l	61	22	of	of	IN
cord-104268-q1jx0n0l	61	23	the	the	DT
cord-104268-q1jx0n0l	61	24	protein	protein	NN
cord-104268-q1jx0n0l	61	25	(	(	-LRB-
cord-104268-q1jx0n0l	61	26	NH2-KPKNWKKFRE	NH2-KPKNWKKFRE	NNP
cord-104268-q1jx0n0l	61	27	-	-	HYPH
cord-104268-q1jx0n0l	61	28	COOH	COOH	NNP
cord-104268-q1jx0n0l	61	29	;	;	:
cord-104268-q1jx0n0l	61	30	obtained	obtain	VBN
cord-104268-q1jx0n0l	61	31	from	from	IN
cord-104268-q1jx0n0l	61	32	the	the	DT
cord-104268-q1jx0n0l	61	33	Sheldon	Sheldon	NNP
cord-104268-q1jx0n0l	61	34	Biotechnology	Biotechnology	NNP
cord-104268-q1jx0n0l	61	35	Centre	Centre	NNP
cord-104268-q1jx0n0l	61	36	,	,	,
cord-104268-q1jx0n0l	61	37	McGill	McGill	NNP
cord-104268-q1jx0n0l	61	38	University	University	NNP
cord-104268-q1jx0n0l	61	39	,	,	,
cord-104268-q1jx0n0l	61	40	Montr6al	Montr6al	NNP
cord-104268-q1jx0n0l	61	41	,	,	,
cord-104268-q1jx0n0l	61	42	Qu6bec	Qu6bec	NNP
cord-104268-q1jx0n0l	61	43	,	,	,
cord-104268-q1jx0n0l	61	44	Canada	Canada	NNP
cord-104268-q1jx0n0l	61	45	)	)	-RRB-
cord-104268-q1jx0n0l	61	46	.	.	.
cord-104268-q1jx0n0l	62	1	Initially	initially	RB
cord-104268-q1jx0n0l	62	2	,	,	,
cord-104268-q1jx0n0l	62	3	rabbits	rabbit	NNS
cord-104268-q1jx0n0l	62	4	were	be	VBD
cord-104268-q1jx0n0l	62	5	injected	inject	VBN
cord-104268-q1jx0n0l	62	6	with	with	IN
cord-104268-q1jx0n0l	62	7	500	500	CD
cord-104268-q1jx0n0l	62	8	~g	~g	NNS
cord-104268-q1jx0n0l	62	9	of	of	IN
cord-104268-q1jx0n0l	62	10	conjugated	conjugated	JJ
cord-104268-q1jx0n0l	62	11	peptide	peptide	NN
cord-104268-q1jx0n0l	62	12	in	in	IN
cord-104268-q1jx0n0l	62	13	Freund	Freund	NNP
cord-104268-q1jx0n0l	62	14	's	's	POS
cord-104268-q1jx0n0l	62	15	complete	complete	JJ
cord-104268-q1jx0n0l	62	16	adjuvant	adjuvant	NN
cord-104268-q1jx0n0l	62	17	,	,	,
cord-104268-q1jx0n0l	62	18	followed	follow	VBN
cord-104268-q1jx0n0l	62	19	by	by	IN
cord-104268-q1jx0n0l	62	20	three	three	CD
cord-104268-q1jx0n0l	62	21	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	62	22	injections	injection	NNS
cord-104268-q1jx0n0l	62	23	with	with	IN
cord-104268-q1jx0n0l	62	24	equivalent	equivalent	JJ
cord-104268-q1jx0n0l	62	25	amounts	amount	NNS
cord-104268-q1jx0n0l	62	26	of	of	IN
cord-104268-q1jx0n0l	62	27	peptide	peptide	NN
cord-104268-q1jx0n0l	62	28	in	in	IN
cord-104268-q1jx0n0l	62	29	Freund	Freund	NNP
cord-104268-q1jx0n0l	62	30	's	's	POS
cord-104268-q1jx0n0l	62	31	incomplete	incomplete	JJ
cord-104268-q1jx0n0l	62	32	adjuvant	adjuvant	NN
cord-104268-q1jx0n0l	62	33	at	at	IN
cord-104268-q1jx0n0l	62	34	3	3	CD
cord-104268-q1jx0n0l	62	35	-	-	SYM
cord-104268-q1jx0n0l	62	36	4-wk	4-wk	CD
cord-104268-q1jx0n0l	62	37	intervals	interval	NNS
cord-104268-q1jx0n0l	62	38	.	.	.
cord-104268-q1jx0n0l	63	1	The	the	DT
cord-104268-q1jx0n0l	63	2	conjugated	conjugated	JJ
cord-104268-q1jx0n0l	63	3	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	63	4	peptide	peptide	NN
cord-104268-q1jx0n0l	63	5	was	be	VBD
cord-104268-q1jx0n0l	63	6	coupled	couple	VBN
cord-104268-q1jx0n0l	63	7	to	to	IN
cord-104268-q1jx0n0l	63	8	cyanogen	cyanogen	NN
cord-104268-q1jx0n0l	63	9	bromide	bromide	NN
cord-104268-q1jx0n0l	63	10	-	-	HYPH
cord-104268-q1jx0n0l	63	11	activated	activate	VBN
cord-104268-q1jx0n0l	63	12	Sepharose	Sepharose	NNP
cord-104268-q1jx0n0l	63	13	CL-6B	CL-6B	NNP
cord-104268-q1jx0n0l	64	1	(	(	-LRB-
cord-104268-q1jx0n0l	64	2	Pharmacia	Pharmacia	NNP
cord-104268-q1jx0n0l	64	3	LKB	LKB	NNP
cord-104268-q1jx0n0l	64	4	Biotechnology	Biotechnology	NNP
cord-104268-q1jx0n0l	64	5	,	,	,
cord-104268-q1jx0n0l	64	6	Inc.	Inc.	NNP
cord-104268-q1jx0n0l	64	7	,	,	,
cord-104268-q1jx0n0l	64	8	Montr6al	Montr6al	NNP
cord-104268-q1jx0n0l	64	9	,	,	,
cord-104268-q1jx0n0l	64	10	Canada	Canada	NNP
cord-104268-q1jx0n0l	64	11	)	)	-RRB-
cord-104268-q1jx0n0l	64	12	and	and	CC
cord-104268-q1jx0n0l	64	13	used	use	VBN
cord-104268-q1jx0n0l	64	14	in	in	IN
cord-104268-q1jx0n0l	64	15	a	a	DT
cord-104268-q1jx0n0l	64	16	column	column	NN
cord-104268-q1jx0n0l	64	17	to	to	IN
cord-104268-q1jx0n0l	64	18	affinity	affinity	NN
cord-104268-q1jx0n0l	64	19	purify	purify	VB
cord-104268-q1jx0n0l	64	20	the	the	DT
cord-104268-q1jx0n0l	64	21	antiserum	antiserum	NN
cord-104268-q1jx0n0l	64	22	as	as	IN
cord-104268-q1jx0n0l	64	23	described	describe	VBN
cord-104268-q1jx0n0l	64	24	by	by	IN
cord-104268-q1jx0n0l	64	25	Raymond	Raymond	NNP
cord-104268-q1jx0n0l	64	26	et	et	NNP
cord-104268-q1jx0n0l	64	27	aL	aL	NNP
cord-104268-q1jx0n0l	64	28	(	(	-LRB-
cord-104268-q1jx0n0l	64	29	1990	1990	CD
cord-104268-q1jx0n0l	64	30	)	)	-RRB-
cord-104268-q1jx0n0l	64	31	.	.	.
cord-104268-q1jx0n0l	65	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	65	2	total	total	JJ
cord-104268-q1jx0n0l	65	3	-	-	HYPH
cord-104268-q1jx0n0l	65	4	ceU	ceu	JJ
cord-104268-q1jx0n0l	65	5	protein	protein	NN
cord-104268-q1jx0n0l	65	6	extracts	extract	NNS
cord-104268-q1jx0n0l	65	7	were	be	VBD
cord-104268-q1jx0n0l	65	8	prepared	prepare	VBN
cord-104268-q1jx0n0l	65	9	from	from	IN
cord-104268-q1jx0n0l	65	10	cultures	culture	NNS
cord-104268-q1jx0n0l	65	11	growing	grow	VBG
cord-104268-q1jx0n0l	65	12	exponentially	exponentially	RB
cord-104268-q1jx0n0l	65	13	in	in	IN
cord-104268-q1jx0n0l	65	14	yeast	yeast	NN
cord-104268-q1jx0n0l	65	15	nitrogen	nitrogen	NN
cord-104268-q1jx0n0l	65	16	base	base	NN
cord-104268-q1jx0n0l	65	17	selective	selective	JJ
cord-104268-q1jx0n0l	65	18	medium	medium	NN
cord-104268-q1jx0n0l	65	19	by	by	IN
cord-104268-q1jx0n0l	65	20	cell	cell	NN
cord-104268-q1jx0n0l	65	21	lysis	lysis	NN
cord-104268-q1jx0n0l	65	22	with	with	IN
cord-104268-q1jx0n0l	65	23	glass	glass	NN
cord-104268-q1jx0n0l	65	24	beads	bead	NNS
cord-104268-q1jx0n0l	65	25	in	in	IN
cord-104268-q1jx0n0l	65	26	the	the	DT
cord-104268-q1jx0n0l	65	27	presence	presence	NN
cord-104268-q1jx0n0l	65	28	of	of	IN
cord-104268-q1jx0n0l	65	29	protease	protease	NN
cord-104268-q1jx0n0l	65	30	inhibitors	inhibitor	NNS
cord-104268-q1jx0n0l	65	31	.	.	.
cord-104268-q1jx0n0l	66	1	Membrane	membrane	NN
cord-104268-q1jx0n0l	66	2	fractions	fraction	NNS
cord-104268-q1jx0n0l	66	3	were	be	VBD
cord-104268-q1jx0n0l	66	4	prepared	prepare	VBN
cord-104268-q1jx0n0l	66	5	as	as	IN
cord-104268-q1jx0n0l	66	6	described	describe	VBN
cord-104268-q1jx0n0l	66	7	(	(	-LRB-
cord-104268-q1jx0n0l	66	8	Nakayama	Nakayama	NNP
cord-104268-q1jx0n0l	66	9	et	et	FW
cord-104268-q1jx0n0l	66	10	al	al	NNP
cord-104268-q1jx0n0l	66	11	.	.	NNP
cord-104268-q1jx0n0l	66	12	,	,	,
cord-104268-q1jx0n0l	66	13	1992	1992	CD
cord-104268-q1jx0n0l	66	14	)	)	-RRB-
cord-104268-q1jx0n0l	66	15	by	by	IN
cord-104268-q1jx0n0l	66	16	centrifuging	centrifuge	VBG
cord-104268-q1jx0n0l	66	17	cell	cell	NN
cord-104268-q1jx0n0l	66	18	lysates	lysate	NNS
cord-104268-q1jx0n0l	66	19	at	at	IN
cord-104268-q1jx0n0l	66	20	10,000	10,000	CD
cord-104268-q1jx0n0l	66	21	g	g	NN
cord-104268-q1jx0n0l	66	22	for	for	IN
cord-104268-q1jx0n0l	66	23	20	20	CD
cord-104268-q1jx0n0l	66	24	min	min	NN
cord-104268-q1jx0n0l	66	25	and	and	CC
cord-104268-q1jx0n0l	66	26	by	by	IN
cord-104268-q1jx0n0l	66	27	centrifuging	centrifuge	VBG
cord-104268-q1jx0n0l	66	28	the	the	DT
cord-104268-q1jx0n0l	66	29	resulting	result	VBG
cord-104268-q1jx0n0l	66	30	supernatant	supernatant	NN
cord-104268-q1jx0n0l	66	31	at	at	IN
cord-104268-q1jx0n0l	66	32	100,000	100,000	CD
cord-104268-q1jx0n0l	66	33	g	g	NN
cord-104268-q1jx0n0l	66	34	for	for	IN
cord-104268-q1jx0n0l	66	35	1	1	CD
cord-104268-q1jx0n0l	66	36	h.	h.	NN
cord-104268-q1jx0n0l	67	1	The	the	DT
cord-104268-q1jx0n0l	67	2	high	high	JJ
cord-104268-q1jx0n0l	67	3	speed	speed	NN
cord-104268-q1jx0n0l	67	4	pellet	pellet	NN
cord-104268-q1jx0n0l	67	5	contained	contain	VBD
cord-104268-q1jx0n0l	67	6	the	the	DT
cord-104268-q1jx0n0l	67	7	insoluble	insoluble	JJ
cord-104268-q1jx0n0l	67	8	membrane	membrane	NN
cord-104268-q1jx0n0l	67	9	fraction	fraction	NN
cord-104268-q1jx0n0l	67	10	.	.	.
cord-104268-q1jx0n0l	68	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	68	2	proteins	protein	NNS
cord-104268-q1jx0n0l	68	3	were	be	VBD
cord-104268-q1jx0n0l	68	4	separated	separate	VBN
cord-104268-q1jx0n0l	68	5	by	by	IN
cord-104268-q1jx0n0l	68	6	SDS	SDS	NNP
cord-104268-q1jx0n0l	68	7	-	-	HYPH
cord-104268-q1jx0n0l	68	8	PAGE	PAGE	NNP
cord-104268-q1jx0n0l	68	9	,	,	,
cord-104268-q1jx0n0l	68	10	and	and	CC
cord-104268-q1jx0n0l	68	11	immunoblots	immunoblot	NNS
cord-104268-q1jx0n0l	68	12	were	be	VBD
cord-104268-q1jx0n0l	68	13	carried	carry	VBN
cord-104268-q1jx0n0l	68	14	out	out	RP
cord-104268-q1jx0n0l	68	15	mainly	mainly	RB
cord-104268-q1jx0n0l	68	16	as	as	IN
cord-104268-q1jx0n0l	68	17	described	describe	VBN
cord-104268-q1jx0n0l	68	18	(	(	-LRB-
cord-104268-q1jx0n0l	68	19	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	68	20	et	et	NNP
cord-104268-q1jx0n0l	68	21	al	al	NNP
cord-104268-q1jx0n0l	68	22	.	.	NNP
cord-104268-q1jx0n0l	68	23	,	,	,
cord-104268-q1jx0n0l	68	24	1995a	1995a	CD
cord-104268-q1jx0n0l	68	25	)	)	-RRB-
cord-104268-q1jx0n0l	68	26	.	.	.
cord-104268-q1jx0n0l	69	1	Briefly	briefly	RB
cord-104268-q1jx0n0l	69	2	,	,	,
cord-104268-q1jx0n0l	69	3	blots	blot	NNS
cord-104268-q1jx0n0l	69	4	were	be	VBD
cord-104268-q1jx0n0l	69	5	treated	treat	VBN
cord-104268-q1jx0n0l	69	6	in	in	IN
cord-104268-q1jx0n0l	69	7	TBST	TBST	NNP
cord-104268-q1jx0n0l	69	8	buffer	buffer	NN
cord-104268-q1jx0n0l	69	9	(	(	-LRB-
cord-104268-q1jx0n0l	69	10	10	10	CD
cord-104268-q1jx0n0l	69	11	mM	mM	NNP
cord-104268-q1jx0n0l	69	12	Tris	Tris	NNP
cord-104268-q1jx0n0l	69	13	,	,	,
cord-104268-q1jx0n0l	69	14	pH	pH	NNP
cord-104268-q1jx0n0l	69	15	8.0	8.0	CD
cord-104268-q1jx0n0l	69	16	,	,	,
cord-104268-q1jx0n0l	69	17	150	150	CD
cord-104268-q1jx0n0l	69	18	mM	mm	CD
cord-104268-q1jx0n0l	69	19	NaC1	NaC1	NNPS
cord-104268-q1jx0n0l	69	20	,	,	,
cord-104268-q1jx0n0l	69	21	0.05	0.05	CD
cord-104268-q1jx0n0l	69	22	%	%	NN
cord-104268-q1jx0n0l	69	23	Tween	Tween	NNP
cord-104268-q1jx0n0l	69	24	20	20	CD
cord-104268-q1jx0n0l	69	25	,	,	,
cord-104268-q1jx0n0l	69	26	5	5	CD
cord-104268-q1jx0n0l	69	27	%	%	NN
cord-104268-q1jx0n0l	69	28	nonfat	nonfat	JJ
cord-104268-q1jx0n0l	69	29	dried	dry	VBD
cord-104268-q1jx0n0l	69	30	milk	milk	NN
cord-104268-q1jx0n0l	69	31	powder	powder	NN
cord-104268-q1jx0n0l	69	32	)	)	-RRB-
cord-104268-q1jx0n0l	69	33	and	and	CC
cord-104268-q1jx0n0l	69	34	subsequently	subsequently	RB
cord-104268-q1jx0n0l	69	35	incubated	incubate	VBN
cord-104268-q1jx0n0l	69	36	with	with	IN
cord-104268-q1jx0n0l	69	37	affinity	affinity	NN
cord-104268-q1jx0n0l	69	38	-	-	HYPH
cord-104268-q1jx0n0l	69	39	purified	purify	VBN
cord-104268-q1jx0n0l	69	40	anti	anti	JJ
cord-104268-q1jx0n0l	69	41	-	-	JJ
cord-104268-q1jx0n0l	69	42	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	69	43	antibodies	antibody	NNS
cord-104268-q1jx0n0l	69	44	in	in	IN
cord-104268-q1jx0n0l	69	45	the	the	DT
cord-104268-q1jx0n0l	69	46	same	same	JJ
cord-104268-q1jx0n0l	69	47	buffer	buffer	NN
cord-104268-q1jx0n0l	69	48	.	.	.
cord-104268-q1jx0n0l	70	1	After	after	IN
cord-104268-q1jx0n0l	70	2	antibody	antibody	NN
cord-104268-q1jx0n0l	70	3	binding	binding	NN
cord-104268-q1jx0n0l	70	4	,	,	,
cord-104268-q1jx0n0l	70	5	membranes	membrane	NNS
cord-104268-q1jx0n0l	70	6	were	be	VBD
cord-104268-q1jx0n0l	70	7	washed	wash	VBN
cord-104268-q1jx0n0l	70	8	in	in	IN
cord-104268-q1jx0n0l	70	9	TBST	TBST	NNP
cord-104268-q1jx0n0l	70	10	and	and	CC
cord-104268-q1jx0n0l	70	11	a	a	DT
cord-104268-q1jx0n0l	70	12	second	second	JJ
cord-104268-q1jx0n0l	70	13	antibody	antibody	NN
cord-104268-q1jx0n0l	70	14	directed	direct	VBN
cord-104268-q1jx0n0l	70	15	against	against	IN
cord-104268-q1jx0n0l	70	16	rabbit	rabbit	NN
cord-104268-q1jx0n0l	70	17	immunoglobulins	immunoglobulin	NNS
cord-104268-q1jx0n0l	70	18	and	and	CC
cord-104268-q1jx0n0l	70	19	conjugated	conjugate	VBN
cord-104268-q1jx0n0l	70	20	with	with	IN
cord-104268-q1jx0n0l	70	21	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	70	22	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	70	23	,	,	,
cord-104268-q1jx0n0l	70	24	was	be	VBD
cord-104268-q1jx0n0l	70	25	then	then	RB
cord-104268-q1jx0n0l	70	26	added	add	VBN
cord-104268-q1jx0n0l	70	27	.	.	.
cord-104268-q1jx0n0l	71	1	The	the	DT
cord-104268-q1jx0n0l	71	2	blots	blot	NNS
cord-104268-q1jx0n0l	71	3	were	be	VBD
cord-104268-q1jx0n0l	71	4	again	again	RB
cord-104268-q1jx0n0l	71	5	washed	wash	VBN
cord-104268-q1jx0n0l	71	6	and	and	CC
cord-104268-q1jx0n0l	71	7	proteins	protein	NNS
cord-104268-q1jx0n0l	71	8	detected	detect	VBD
cord-104268-q1jx0n0l	71	9	using	use	VBG
cord-104268-q1jx0n0l	71	10	an	an	DT
cord-104268-q1jx0n0l	71	11	enhanced	enhance	VBN
cord-104268-q1jx0n0l	71	12	chemiluminescence	chemiluminescence	NN
cord-104268-q1jx0n0l	71	13	procedure	procedure	NN
cord-104268-q1jx0n0l	71	14	(	(	-LRB-
cord-104268-q1jx0n0l	71	15	Amersham	Amersham	NNP
cord-104268-q1jx0n0l	71	16	Canada	Canada	NNP
cord-104268-q1jx0n0l	71	17	,	,	,
cord-104268-q1jx0n0l	71	18	OakviUe	OakviUe	NNP
cord-104268-q1jx0n0l	71	19	,	,	,
cord-104268-q1jx0n0l	71	20	Ontario	Ontario	NNP
cord-104268-q1jx0n0l	71	21	)	)	-RRB-
cord-104268-q1jx0n0l	71	22	.	.	.
cord-104268-q1jx0n0l	72	1	Analysis	analysis	NN
cord-104268-q1jx0n0l	72	2	of	of	IN
cord-104268-q1jx0n0l	72	3	[	[	-LRB-
cord-104268-q1jx0n0l	72	4	35S]methionine	35s]methionine	CD
cord-104268-q1jx0n0l	72	5	-	-	HYPH
cord-104268-q1jx0n0l	72	6	labeled	label	VBN
cord-104268-q1jx0n0l	72	7	proteins	protein	NNS
cord-104268-q1jx0n0l	72	8	was	be	VBD
cord-104268-q1jx0n0l	72	9	as	as	IN
cord-104268-q1jx0n0l	72	10	described	describe	VBN
cord-104268-q1jx0n0l	72	11	(	(	-LRB-
cord-104268-q1jx0n0l	72	12	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	72	13	and	and	CC
cord-104268-q1jx0n0l	72	14	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	72	15	,	,	,
cord-104268-q1jx0n0l	72	16	1989	1989	CD
cord-104268-q1jx0n0l	72	17	)	)	-RRB-
cord-104268-q1jx0n0l	72	18	.	.	.
cord-104268-q1jx0n0l	73	1	Briefly	briefly	RB
cord-104268-q1jx0n0l	73	2	,	,	,
cord-104268-q1jx0n0l	73	3	cells	cell	NNS
cord-104268-q1jx0n0l	73	4	were	be	VBD
cord-104268-q1jx0n0l	73	5	grown	grow	VBN
cord-104268-q1jx0n0l	73	6	to	to	IN
cord-104268-q1jx0n0l	73	7	an	an	DT
cord-104268-q1jx0n0l	73	8	OD600	od600	NN
cord-104268-q1jx0n0l	73	9	of	of	IN
cord-104268-q1jx0n0l	73	10	0.4	0.4	CD
cord-104268-q1jx0n0l	73	11	-	-	SYM
cord-104268-q1jx0n0l	73	12	0.45	0.45	CD
cord-104268-q1jx0n0l	73	13	in	in	IN
cord-104268-q1jx0n0l	73	14	selective	selective	JJ
cord-104268-q1jx0n0l	73	15	medium	medium	NN
cord-104268-q1jx0n0l	73	16	(	(	-LRB-
cord-104268-q1jx0n0l	73	17	YNB	YNB	NNP
cord-104268-q1jx0n0l	73	18	)	)	-RRB-
cord-104268-q1jx0n0l	73	19	and	and	CC
cord-104268-q1jx0n0l	73	20	then	then	RB
cord-104268-q1jx0n0l	73	21	labeled	label	VBN
cord-104268-q1jx0n0l	73	22	with	with	IN
cord-104268-q1jx0n0l	73	23	Trans35S	Trans35S	NNP
cord-104268-q1jx0n0l	73	24	(	(	-LRB-
cord-104268-q1jx0n0l	73	25	100	100	CD
cord-104268-q1jx0n0l	73	26	~Ci	~ci	CD
cord-104268-q1jx0n0l	73	27	;	;	:
cord-104268-q1jx0n0l	73	28	ICN	ICN	NNP
cord-104268-q1jx0n0l	73	29	Biochemicals	Biochemicals	NNPS
cord-104268-q1jx0n0l	73	30	Inc.	Inc.	NNP
cord-104268-q1jx0n0l	73	31	,	,	,
cord-104268-q1jx0n0l	73	32	Montr6al	Montr6al	NNP
cord-104268-q1jx0n0l	73	33	,	,	,
cord-104268-q1jx0n0l	73	34	Canada	Canada	NNP
cord-104268-q1jx0n0l	73	35	)	)	-RRB-
cord-104268-q1jx0n0l	73	36	for	for	IN
cord-104268-q1jx0n0l	73	37	10	10	CD
cord-104268-q1jx0n0l	73	38	min	min	NN
cord-104268-q1jx0n0l	73	39	at	at	IN
cord-104268-q1jx0n0l	73	40	30	30	CD
cord-104268-q1jx0n0l	73	41	°	°	NN
cord-104268-q1jx0n0l	73	42	C	c	NN
cord-104268-q1jx0n0l	73	43	.	.	.
cord-104268-q1jx0n0l	74	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	74	2	cells	cell	NNS
cord-104268-q1jx0n0l	74	3	treated	treat	VBN
cord-104268-q1jx0n0l	74	4	with	with	IN
cord-104268-q1jx0n0l	74	5	tunicamycin	tunicamycin	NNS
cord-104268-q1jx0n0l	74	6	(	(	-LRB-
cord-104268-q1jx0n0l	74	7	10	10	CD
cord-104268-q1jx0n0l	74	8	~g	~g	NNP
cord-104268-q1jx0n0l	74	9	m1	m1	NNP
cord-104268-q1jx0n0l	74	10	-	-	HYPH
cord-104268-q1jx0n0l	74	11	1	1	NNP
cord-104268-q1jx0n0l	74	12	)	)	-RRB-
cord-104268-q1jx0n0l	74	13	were	be	VBD
cord-104268-q1jx0n0l	74	14	preincubated	preincubate	VBN
cord-104268-q1jx0n0l	74	15	before	before	IN
cord-104268-q1jx0n0l	74	16	radiolabeling	radiolabele	VBG
cord-104268-q1jx0n0l	74	17	for	for	IN
cord-104268-q1jx0n0l	74	18	30	30	CD
cord-104268-q1jx0n0l	74	19	min	min	NN
cord-104268-q1jx0n0l	74	20	at	at	IN
cord-104268-q1jx0n0l	74	21	30	30	CD
cord-104268-q1jx0n0l	74	22	°	°	NN
cord-104268-q1jx0n0l	74	23	C	c	NN
cord-104268-q1jx0n0l	74	24	.	.	.
cord-104268-q1jx0n0l	75	1	Tunicamycin	Tunicamycin	NNP
cord-104268-q1jx0n0l	75	2	is	be	VBZ
cord-104268-q1jx0n0l	75	3	a	a	DT
cord-104268-q1jx0n0l	75	4	hydrophobic	hydrophobic	JJ
cord-104268-q1jx0n0l	75	5	analogue	analogue	NN
cord-104268-q1jx0n0l	75	6	of	of	IN
cord-104268-q1jx0n0l	75	7	UDP	UDP	NNP
cord-104268-q1jx0n0l	75	8	-	-	HYPH
cord-104268-q1jx0n0l	75	9	N	N	NNP
cord-104268-q1jx0n0l	75	10	-	-	HYPH
cord-104268-q1jx0n0l	75	11	acetylglucosamine	acetylglucosamine	NNP
cord-104268-q1jx0n0l	75	12	that	that	WDT
cord-104268-q1jx0n0l	75	13	blocks	block	VBZ
cord-104268-q1jx0n0l	75	14	the	the	DT
cord-104268-q1jx0n0l	75	15	addition	addition	NN
cord-104268-q1jx0n0l	75	16	of	of	IN
cord-104268-q1jx0n0l	75	17	N	N	NNP
cord-104268-q1jx0n0l	75	18	-	-	HYPH
cord-104268-q1jx0n0l	75	19	acetylglucosamine	acetylglucosamine	NNP
cord-104268-q1jx0n0l	75	20	to	to	IN
cord-104268-q1jx0n0l	75	21	dolichol	dolichol	JJ
cord-104268-q1jx0n0l	75	22	phosphate	phosphate	NN
cord-104268-q1jx0n0l	75	23	,	,	,
cord-104268-q1jx0n0l	75	24	the	the	DT
cord-104268-q1jx0n0l	75	25	first	first	JJ
cord-104268-q1jx0n0l	75	26	step	step	NN
cord-104268-q1jx0n0l	75	27	in	in	IN
cord-104268-q1jx0n0l	75	28	N	n	CD
cord-104268-q1jx0n0l	75	29	-	-	HYPH
cord-104268-q1jx0n0l	75	30	linked	link	VBN
cord-104268-q1jx0n0l	75	31	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	75	32	formation	formation	NN
cord-104268-q1jx0n0l	75	33	.	.	.
cord-104268-q1jx0n0l	76	1	Chase	chase	NN
cord-104268-q1jx0n0l	76	2	conditions	condition	NNS
cord-104268-q1jx0n0l	76	3	were	be	VBD
cord-104268-q1jx0n0l	76	4	achieved	achieve	VBN
cord-104268-q1jx0n0l	76	5	by	by	IN
cord-104268-q1jx0n0l	76	6	addition	addition	NN
cord-104268-q1jx0n0l	76	7	of	of	IN
cord-104268-q1jx0n0l	76	8	L	l	NN
cord-104268-q1jx0n0l	76	9	-	-	HYPH
cord-104268-q1jx0n0l	76	10	methionine	methionine	NNP
cord-104268-q1jx0n0l	76	11	and	and	CC
cord-104268-q1jx0n0l	76	12	L	l	NN
cord-104268-q1jx0n0l	76	13	-	-	HYPH
cord-104268-q1jx0n0l	76	14	cysteine	cysteine	NN
cord-104268-q1jx0n0l	76	15	to	to	IN
cord-104268-q1jx0n0l	76	16	a	a	DT
cord-104268-q1jx0n0l	76	17	final	final	JJ
cord-104268-q1jx0n0l	76	18	concentration	concentration	NN
cord-104268-q1jx0n0l	76	19	of	of	IN
cord-104268-q1jx0n0l	76	20	i	i	PRP
cord-104268-q1jx0n0l	76	21	mM.	mm.	VBP
cord-104268-q1jx0n0l	76	22	txl,2-mannosyltransferase	txl,2-mannosyltransferase	NNP
cord-104268-q1jx0n0l	76	23	activity	activity	NN
cord-104268-q1jx0n0l	76	24	assays	assay	NNS
cord-104268-q1jx0n0l	76	25	were	be	VBD
cord-104268-q1jx0n0l	76	26	performed	perform	VBN
cord-104268-q1jx0n0l	76	27	essentially	essentially	RB
cord-104268-q1jx0n0l	76	28	as	as	IN
cord-104268-q1jx0n0l	76	29	described	describe	VBN
cord-104268-q1jx0n0l	76	30	(	(	-LRB-
cord-104268-q1jx0n0l	76	31	Lewis	Lewis	NNP
cord-104268-q1jx0n0l	76	32	and	and	CC
cord-104268-q1jx0n0l	76	33	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	76	34	,	,	,
cord-104268-q1jx0n0l	76	35	1991	1991	CD
cord-104268-q1jx0n0l	76	36	;	;	:
cord-104268-q1jx0n0l	76	37	Hhusler	Hhusler	NNP
cord-104268-q1jx0n0l	76	38	and	and	CC
cord-104268-q1jx0n0l	76	39	Robbins	Robbins	NNP
cord-104268-q1jx0n0l	76	40	,	,	,
cord-104268-q1jx0n0l	76	41	1992	1992	CD
cord-104268-q1jx0n0l	76	42	)	)	-RRB-
cord-104268-q1jx0n0l	76	43	.	.	.
cord-104268-q1jx0n0l	77	1	S.	S.	NNP
cord-104268-q1jx0n0l	77	2	cerevisiae	cerevisiae	NN
cord-104268-q1jx0n0l	77	3	cells	cell	NNS
cord-104268-q1jx0n0l	77	4	(	(	-LRB-
cord-104268-q1jx0n0l	77	5	$	$	$
cord-104268-q1jx0n0l	77	6	86	86	CD
cord-104268-q1jx0n0l	77	7	background	background	NN
cord-104268-q1jx0n0l	77	8	)	)	-RRB-
cord-104268-q1jx0n0l	77	9	were	be	VBD
cord-104268-q1jx0n0l	77	10	grown	grow	VBN
cord-104268-q1jx0n0l	77	11	in	in	IN
cord-104268-q1jx0n0l	77	12	selective	selective	JJ
cord-104268-q1jx0n0l	77	13	medium	medium	NN
cord-104268-q1jx0n0l	77	14	to	to	IN
cord-104268-q1jx0n0l	77	15	an	an	DT
cord-104268-q1jx0n0l	77	16	OD600	od600	NN
cord-104268-q1jx0n0l	77	17	of	of	IN
cord-104268-q1jx0n0l	77	18	0.8	0.8	CD
cord-104268-q1jx0n0l	77	19	-	-	SYM
cord-104268-q1jx0n0l	77	20	0.9	0.9	CD
cord-104268-q1jx0n0l	77	21	and	and	CC
cord-104268-q1jx0n0l	77	22	lysed	lyse	VBN
cord-104268-q1jx0n0l	77	23	with	with	IN
cord-104268-q1jx0n0l	77	24	glass	glass	NN
cord-104268-q1jx0n0l	77	25	beads	bead	NNS
cord-104268-q1jx0n0l	77	26	in	in	IN
cord-104268-q1jx0n0l	77	27	the	the	DT
cord-104268-q1jx0n0l	77	28	presence	presence	NN
cord-104268-q1jx0n0l	77	29	of	of	IN
cord-104268-q1jx0n0l	77	30	protease	protease	NN
cord-104268-q1jx0n0l	77	31	inhibitors	inhibitor	NNS
cord-104268-q1jx0n0l	77	32	.	.	.
cord-104268-q1jx0n0l	78	1	High	high	JJ
cord-104268-q1jx0n0l	78	2	speed	speed	NN
cord-104268-q1jx0n0l	78	3	pellet	pellet	NN
cord-104268-q1jx0n0l	78	4	fractions	fraction	NNS
cord-104268-q1jx0n0l	78	5	including	include	VBG
cord-104268-q1jx0n0l	78	6	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	78	7	and	and	CC
cord-104268-q1jx0n0l	78	8	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	78	9	membranes	membrane	NNS
cord-104268-q1jx0n0l	78	10	were	be	VBD
cord-104268-q1jx0n0l	78	11	prepared	prepare	VBN
cord-104268-q1jx0n0l	78	12	by	by	IN
cord-104268-q1jx0n0l	78	13	centrifuging	centrifuge	VBG
cord-104268-q1jx0n0l	78	14	cell	cell	NN
cord-104268-q1jx0n0l	78	15	lysates	lysate	NNS
cord-104268-q1jx0n0l	78	16	at	at	IN
cord-104268-q1jx0n0l	78	17	1,1300	1,1300	NNP
cord-104268-q1jx0n0l	78	18	g	g	NN
cord-104268-q1jx0n0l	78	19	for	for	IN
cord-104268-q1jx0n0l	78	20	20	20	CD
cord-104268-q1jx0n0l	78	21	min	min	NN
cord-104268-q1jx0n0l	78	22	and	and	CC
cord-104268-q1jx0n0l	78	23	by	by	IN
cord-104268-q1jx0n0l	78	24	centrifuging	centrifuge	VBG
cord-104268-q1jx0n0l	78	25	the	the	DT
cord-104268-q1jx0n0l	78	26	resulting	result	VBG
cord-104268-q1jx0n0l	78	27	supernatant	supernatant	NN
cord-104268-q1jx0n0l	78	28	at	at	IN
cord-104268-q1jx0n0l	78	29	100,000	100,000	CD
cord-104268-q1jx0n0l	78	30	g	g	NN
cord-104268-q1jx0n0l	78	31	for	for	IN
cord-104268-q1jx0n0l	78	32	1	1	CD
cord-104268-q1jx0n0l	78	33	h	h	NN
cord-104268-q1jx0n0l	78	34	at	at	IN
cord-104268-q1jx0n0l	78	35	4	4	CD
cord-104268-q1jx0n0l	78	36	°	°	NN
cord-104268-q1jx0n0l	78	37	C	c	NN
cord-104268-q1jx0n0l	78	38	.	.	.
cord-104268-q1jx0n0l	79	1	Incubation	incubation	NN
cord-104268-q1jx0n0l	79	2	mixtures	mixture	NNS
cord-104268-q1jx0n0l	79	3	contained	contain	VBD
cord-104268-q1jx0n0l	79	4	50	50	CD
cord-104268-q1jx0n0l	79	5	mM	mM	NNP
cord-104268-q1jx0n0l	79	6	Hepes	Hepes	NNP
cord-104268-q1jx0n0l	79	7	,	,	,
cord-104268-q1jx0n0l	79	8	pH	pH	NNP
cord-104268-q1jx0n0l	79	9	7.2	7.2	CD
cord-104268-q1jx0n0l	79	10	,	,	,
cord-104268-q1jx0n0l	79	11	10	10	CD
cord-104268-q1jx0n0l	79	12	mM	mm	CD
cord-104268-q1jx0n0l	79	13	MnCI2	MnCI2	NNP
cord-104268-q1jx0n0l	79	14	,	,	,
cord-104268-q1jx0n0l	79	15	0.1	0.1	CD
cord-104268-q1jx0n0l	79	16	%	%	NN
cord-104268-q1jx0n0l	79	17	Triton	Triton	NNP
cord-104268-q1jx0n0l	79	18	X-100	x-100	NN
cord-104268-q1jx0n0l	79	19	,	,	,
cord-104268-q1jx0n0l	79	20	0.2	0.2	CD
cord-104268-q1jx0n0l	79	21	mM	mm	CD
cord-104268-q1jx0n0l	79	22	GDP-[lgC]mannose	gdp-[lgc]mannose	NN
cord-104268-q1jx0n0l	79	23	,	,	,
cord-104268-q1jx0n0l	79	24	and	and	CC
cord-104268-q1jx0n0l	79	25	10	10	CD
cord-104268-q1jx0n0l	79	26	mM	mm	CD
cord-104268-q1jx0n0l	79	27	C~-D	C~-D	NNP
cord-104268-q1jx0n0l	79	28	-	-	HYPH
cord-104268-q1jx0n0l	79	29	methylmanno	methylmanno	NN
cord-104268-q1jx0n0l	79	30	-	-	HYPH
cord-104268-q1jx0n0l	79	31	pyranoside	pyranoside	NN
cord-104268-q1jx0n0l	79	32	as	as	IN
cord-104268-q1jx0n0l	79	33	an	an	DT
cord-104268-q1jx0n0l	79	34	acceptor	acceptor	NN
cord-104268-q1jx0n0l	79	35	(	(	-LRB-
cord-104268-q1jx0n0l	79	36	Sigma	Sigma	NNP
cord-104268-q1jx0n0l	79	37	Chemical	Chemical	NNP
cord-104268-q1jx0n0l	79	38	Co.	Co.	NNP
cord-104268-q1jx0n0l	79	39	)	)	-RRB-
cord-104268-q1jx0n0l	79	40	and	and	CC
cord-104268-q1jx0n0l	79	41	10	10	CD
cord-104268-q1jx0n0l	79	42	p,1	p,1	NN
cord-104268-q1jx0n0l	79	43	of	of	IN
cord-104268-q1jx0n0l	79	44	membrane	membrane	NN
cord-104268-q1jx0n0l	79	45	fraction	fraction	NN
cord-104268-q1jx0n0l	79	46	in	in	IN
cord-104268-q1jx0n0l	79	47	a	a	DT
cord-104268-q1jx0n0l	79	48	total	total	JJ
cord-104268-q1jx0n0l	79	49	vol	vol	NN
cord-104268-q1jx0n0l	79	50	of	of	IN
cord-104268-q1jx0n0l	79	51	20	20	CD
cord-104268-q1jx0n0l	79	52	tzl	tzl	NN
cord-104268-q1jx0n0l	79	53	.	.	.
cord-104268-q1jx0n0l	80	1	Reaction	reaction	NN
cord-104268-q1jx0n0l	80	2	mixtures	mixture	NNS
cord-104268-q1jx0n0l	80	3	were	be	VBD
cord-104268-q1jx0n0l	80	4	incubated	incubate	VBN
cord-104268-q1jx0n0l	80	5	for	for	IN
cord-104268-q1jx0n0l	80	6	30	30	CD
cord-104268-q1jx0n0l	80	7	-	-	SYM
cord-104268-q1jx0n0l	80	8	-60	-60	NNS
cord-104268-q1jx0n0l	80	9	min	min	NN
cord-104268-q1jx0n0l	80	10	at	at	IN
cord-104268-q1jx0n0l	80	11	30	30	CD
cord-104268-q1jx0n0l	80	12	°	°	NN
cord-104268-q1jx0n0l	80	13	C	c	NN
cord-104268-q1jx0n0l	80	14	and	and	CC
cord-104268-q1jx0n0l	80	15	then	then	RB
cord-104268-q1jx0n0l	80	16	passed	pass	VBD
cord-104268-q1jx0n0l	80	17	through	through	IN
cord-104268-q1jx0n0l	80	18	a	a	DT
cord-104268-q1jx0n0l	80	19	resin	resin	NN
cord-104268-q1jx0n0l	80	20	(	(	-LRB-
cord-104268-q1jx0n0l	80	21	AG1-X4	ag1-x4	NN
cord-104268-q1jx0n0l	80	22	;	;	:
cord-104268-q1jx0n0l	80	23	Bio	Bio	NNP
cord-104268-q1jx0n0l	80	24	-	-	HYPH
cord-104268-q1jx0n0l	80	25	Rad	Rad	NNP
cord-104268-q1jx0n0l	80	26	Laboratories	Laboratories	NNP
cord-104268-q1jx0n0l	80	27	,	,	,
cord-104268-q1jx0n0l	80	28	Hercules	Hercules	NNP
cord-104268-q1jx0n0l	80	29	,	,	,
cord-104268-q1jx0n0l	80	30	CA	CA	NNP
cord-104268-q1jx0n0l	80	31	)	)	-RRB-
cord-104268-q1jx0n0l	80	32	column	column	NN
cord-104268-q1jx0n0l	80	33	to	to	TO
cord-104268-q1jx0n0l	80	34	remove	remove	VB
cord-104268-q1jx0n0l	80	35	unreacted	unreacted	JJ
cord-104268-q1jx0n0l	80	36	GDP	gdp	NN
cord-104268-q1jx0n0l	80	37	-	-	HYPH
cord-104268-q1jx0n0l	80	38	mannose	mannose	NN
cord-104268-q1jx0n0l	80	39	.	.	.
cord-104268-q1jx0n0l	81	1	Neutral	neutral	JJ
cord-104268-q1jx0n0l	81	2	products	product	NNS
cord-104268-q1jx0n0l	81	3	were	be	VBD
cord-104268-q1jx0n0l	81	4	eluted	elute	VBN
cord-104268-q1jx0n0l	81	5	with	with	IN
cord-104268-q1jx0n0l	81	6	1	1	CD
cord-104268-q1jx0n0l	81	7	ml	ml	NNS
cord-104268-q1jx0n0l	81	8	of	of	IN
cord-104268-q1jx0n0l	81	9	water	water	NN
cord-104268-q1jx0n0l	81	10	and	and	CC
cord-104268-q1jx0n0l	81	11	radioactivity	radioactivity	NN
cord-104268-q1jx0n0l	81	12	was	be	VBD
cord-104268-q1jx0n0l	81	13	measured	measure	VBN
cord-104268-q1jx0n0l	81	14	.	.	.
cord-104268-q1jx0n0l	82	1	Control	control	NN
cord-104268-q1jx0n0l	82	2	assays	assay	NNS
cord-104268-q1jx0n0l	82	3	were	be	VBD
cord-104268-q1jx0n0l	82	4	conducted	conduct	VBN
cord-104268-q1jx0n0l	82	5	in	in	IN
cord-104268-q1jx0n0l	82	6	which	which	WDT
cord-104268-q1jx0n0l	82	7	the	the	DT
cord-104268-q1jx0n0l	82	8	saccharide	saccharide	NN
cord-104268-q1jx0n0l	82	9	acceptor	acceptor	NN
cord-104268-q1jx0n0l	82	10	was	be	VBD
cord-104268-q1jx0n0l	82	11	omitted	omit	VBN
cord-104268-q1jx0n0l	82	12	and	and	CC
cord-104268-q1jx0n0l	82	13	counts	count	NNS
cord-104268-q1jx0n0l	82	14	obtained	obtain	VBN
cord-104268-q1jx0n0l	82	15	in	in	IN
cord-104268-q1jx0n0l	82	16	these	these	DT
cord-104268-q1jx0n0l	82	17	assays	assay	NNS
cord-104268-q1jx0n0l	82	18	were	be	VBD
cord-104268-q1jx0n0l	82	19	subtracted	subtract	VBN
cord-104268-q1jx0n0l	82	20	from	from	IN
cord-104268-q1jx0n0l	82	21	values	value	NNS
cord-104268-q1jx0n0l	82	22	obtained	obtain	VBN
cord-104268-q1jx0n0l	82	23	in	in	IN
cord-104268-q1jx0n0l	82	24	assays	assay	NNS
cord-104268-q1jx0n0l	82	25	made	make	VBN
cord-104268-q1jx0n0l	82	26	with	with	IN
cord-104268-q1jx0n0l	82	27	the	the	DT
cord-104268-q1jx0n0l	82	28	sugar	sugar	NN
cord-104268-q1jx0n0l	82	29	acceptor	acceptor	NN
cord-104268-q1jx0n0l	82	30	.	.	.
cord-104268-q1jx0n0l	83	1	Enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	83	2	activities	activity	NNS
cord-104268-q1jx0n0l	83	3	(	(	-LRB-
cord-104268-q1jx0n0l	83	4	see	see	VB
cord-104268-q1jx0n0l	83	5	Fig	Fig	NNP
cord-104268-q1jx0n0l	83	6	.	.	.
cord-104268-q1jx0n0l	83	7	9	9	CD
cord-104268-q1jx0n0l	83	8	)	)	-RRB-
cord-104268-q1jx0n0l	83	9	are	be	VBP
cord-104268-q1jx0n0l	83	10	expressed	express	VBN
cord-104268-q1jx0n0l	83	11	as	as	IN
cord-104268-q1jx0n0l	83	12	percentages	percentage	NNS
cord-104268-q1jx0n0l	83	13	of	of	IN
cord-104268-q1jx0n0l	83	14	specific	specific	JJ
cord-104268-q1jx0n0l	83	15	activity	activity	NN
cord-104268-q1jx0n0l	83	16	(	(	-LRB-
cord-104268-q1jx0n0l	83	17	mmol	mmol	NNP
cord-104268-q1jx0n0l	83	18	/	/	SYM
cord-104268-q1jx0n0l	83	19	h	h	NN
cord-104268-q1jx0n0l	83	20	/	/	SYM
cord-104268-q1jx0n0l	83	21	mg	mg	NN
cord-104268-q1jx0n0l	83	22	of	of	IN
cord-104268-q1jx0n0l	83	23	membrane	membrane	NN
cord-104268-q1jx0n0l	83	24	protein	protein	NN
cord-104268-q1jx0n0l	83	25	)	)	-RRB-
cord-104268-q1jx0n0l	83	26	for	for	IN
cord-104268-q1jx0n0l	83	27	each	each	DT
cord-104268-q1jx0n0l	83	28	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	83	29	protein	protein	NN
cord-104268-q1jx0n0l	83	30	.	.	.
cord-104268-q1jx0n0l	84	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	84	2	cells	cell	NNS
cord-104268-q1jx0n0l	84	3	were	be	VBD
cord-104268-q1jx0n0l	84	4	grown	grow	VBN
cord-104268-q1jx0n0l	84	5	in	in	IN
cord-104268-q1jx0n0l	84	6	selective	selective	JJ
cord-104268-q1jx0n0l	84	7	medium	medium	NN
cord-104268-q1jx0n0l	84	8	to	to	IN
cord-104268-q1jx0n0l	84	9	an	an	DT
cord-104268-q1jx0n0l	84	10	OD600	od600	NN
cord-104268-q1jx0n0l	84	11	of	of	IN
cord-104268-q1jx0n0l	84	12	0.5	0.5	CD
cord-104268-q1jx0n0l	84	13	-	-	SYM
cord-104268-q1jx0n0l	84	14	0.7	0.7	CD
cord-104268-q1jx0n0l	84	15	.	.	.
cord-104268-q1jx0n0l	85	1	and	and	CC
cord-104268-q1jx0n0l	85	2	immediately	immediately	RB
cord-104268-q1jx0n0l	85	3	fixed	fix	VBN
cord-104268-q1jx0n0l	85	4	by	by	IN
cord-104268-q1jx0n0l	85	5	the	the	DT
cord-104268-q1jx0n0l	85	6	addition	addition	NN
cord-104268-q1jx0n0l	85	7	to	to	IN
cord-104268-q1jx0n0l	85	8	cultures	culture	NNS
cord-104268-q1jx0n0l	85	9	of	of	IN
cord-104268-q1jx0n0l	85	10	potassium	potassium	NN
cord-104268-q1jx0n0l	85	11	-	-	HYPH
cord-104268-q1jx0n0l	85	12	phosphate	phosphate	NN
cord-104268-q1jx0n0l	85	13	(	(	-LRB-
cord-104268-q1jx0n0l	85	14	pH	pH	NNP
cord-104268-q1jx0n0l	85	15	6.5	6.5	CD
cord-104268-q1jx0n0l	85	16	)	)	-RRB-
cord-104268-q1jx0n0l	85	17	to	to	IN
cord-104268-q1jx0n0l	85	18	0.1	0.1	CD
cord-104268-q1jx0n0l	85	19	M	m	NN
cord-104268-q1jx0n0l	85	20	,	,	,
cord-104268-q1jx0n0l	85	21	and	and	CC
cord-104268-q1jx0n0l	85	22	formaldehyde	formaldehyde	NN
cord-104268-q1jx0n0l	85	23	to	to	IN
cord-104268-q1jx0n0l	85	24	3.7	3.7	CD
cord-104268-q1jx0n0l	85	25	%	%	NN
cord-104268-q1jx0n0l	85	26	.	.	.
cord-104268-q1jx0n0l	86	1	After	after	IN
cord-104268-q1jx0n0l	86	2	gentle	gentle	JJ
cord-104268-q1jx0n0l	86	3	agitation	agitation	NN
cord-104268-q1jx0n0l	86	4	for	for	IN
cord-104268-q1jx0n0l	86	5	30	30	CD
cord-104268-q1jx0n0l	86	6	rain	rain	NN
cord-104268-q1jx0n0l	86	7	,	,	,
cord-104268-q1jx0n0l	86	8	cells	cell	NNS
cord-104268-q1jx0n0l	86	9	were	be	VBD
cord-104268-q1jx0n0l	86	10	pelleted	pellete	VBN
cord-104268-q1jx0n0l	86	11	and	and	CC
cord-104268-q1jx0n0l	86	12	resuspended	resuspend	VBN
cord-104268-q1jx0n0l	86	13	in	in	IN
cord-104268-q1jx0n0l	86	14	a	a	DT
cord-104268-q1jx0n0l	86	15	fixation	fixation	NN
cord-104268-q1jx0n0l	86	16	buffer	buffer	NN
cord-104268-q1jx0n0l	86	17	containing	contain	VBG
cord-104268-q1jx0n0l	86	18	0.1	0.1	CD
cord-104268-q1jx0n0l	86	19	M	M	NNP
cord-104268-q1jx0n0l	86	20	potassium	potassium	NN
cord-104268-q1jx0n0l	86	21	-	-	HYPH
cord-104268-q1jx0n0l	86	22	phosphate	phosphate	NN
cord-104268-q1jx0n0l	86	23	(	(	-LRB-
cord-104268-q1jx0n0l	86	24	pH	pH	NNP
cord-104268-q1jx0n0l	86	25	6.5	6.5	CD
cord-104268-q1jx0n0l	86	26	)	)	-RRB-
cord-104268-q1jx0n0l	86	27	and	and	CC
cord-104268-q1jx0n0l	86	28	4.5	4.5	CD
cord-104268-q1jx0n0l	86	29	%	%	NN
cord-104268-q1jx0n0l	86	30	formaldehyde	formaldehyde	NN
cord-104268-q1jx0n0l	86	31	.	.	.
cord-104268-q1jx0n0l	87	1	Cells	cell	NNS
cord-104268-q1jx0n0l	87	2	were	be	VBD
cord-104268-q1jx0n0l	87	3	further	further	RB
cord-104268-q1jx0n0l	87	4	fixed	fix	VBN
cord-104268-q1jx0n0l	87	5	for	for	IN
cord-104268-q1jx0n0l	87	6	another	another	DT
cord-104268-q1jx0n0l	87	7	30	30	CD
cord-104268-q1jx0n0l	87	8	rain	rain	NN
cord-104268-q1jx0n0l	87	9	.	.	.
cord-104268-q1jx0n0l	88	1	Formaldehyde	formaldehyde	NN
cord-104268-q1jx0n0l	88	2	-	-	HYPH
cord-104268-q1jx0n0l	88	3	treated	treat	VBN
cord-104268-q1jx0n0l	88	4	ceils	ceil	NNS
cord-104268-q1jx0n0l	88	5	were	be	VBD
cord-104268-q1jx0n0l	88	6	washed	wash	VBN
cord-104268-q1jx0n0l	88	7	with	with	IN
cord-104268-q1jx0n0l	88	8	0.1	0.1	CD
cord-104268-q1jx0n0l	88	9	M	M	NNP
cord-104268-q1jx0n0l	88	10	potassium	potassium	NN
cord-104268-q1jx0n0l	88	11	-	-	HYPH
cord-104268-q1jx0n0l	88	12	phosphate	phosphate	NN
cord-104268-q1jx0n0l	88	13	(	(	-LRB-
cord-104268-q1jx0n0l	88	14	pH	pH	NNP
cord-104268-q1jx0n0l	88	15	6.5	6.5	CD
cord-104268-q1jx0n0l	88	16	)	)	-RRB-
cord-104268-q1jx0n0l	88	17	buffer	buffer	NN
cord-104268-q1jx0n0l	88	18	,	,	,
cord-104268-q1jx0n0l	88	19	resuspended	resuspend	VBN
cord-104268-q1jx0n0l	88	20	in	in	IN
cord-104268-q1jx0n0l	88	21	a	a	DT
cord-104268-q1jx0n0l	88	22	solution	solution	NN
cord-104268-q1jx0n0l	88	23	of	of	IN
cord-104268-q1jx0n0l	88	24	IOO	IOO	NNP
cord-104268-q1jx0n0l	88	25	mM	mM	NNP
cord-104268-q1jx0n0l	88	26	Tris	Tris	NNP
cord-104268-q1jx0n0l	88	27	-	-	HYPH
cord-104268-q1jx0n0l	88	28	HC1	HC1	NNP
cord-104268-q1jx0n0l	88	29	,	,	,
cord-104268-q1jx0n0l	88	30	pH	pH	NNP
cord-104268-q1jx0n0l	88	31	8	8	CD
cord-104268-q1jx0n0l	88	32	,	,	,
cord-104268-q1jx0n0l	88	33	25	25	CD
cord-104268-q1jx0n0l	88	34	mM	mm	CD
cord-104268-q1jx0n0l	88	35	DTr	DTr	NNS
cord-104268-q1jx0n0l	88	36	,	,	,
cord-104268-q1jx0n0l	88	37	5	5	CD
cord-104268-q1jx0n0l	88	38	mM	mm	CD
cord-104268-q1jx0n0l	88	39	EDTA	EDTA	NNP
cord-104268-q1jx0n0l	88	40	,	,	,
cord-104268-q1jx0n0l	88	41	and	and	CC
cord-104268-q1jx0n0l	88	42	1.2	1.2	CD
cord-104268-q1jx0n0l	88	43	M	m	NN
cord-104268-q1jx0n0l	88	44	sorbitol	sorbitol	NN
cord-104268-q1jx0n0l	88	45	and	and	CC
cord-104268-q1jx0n0l	88	46	incubated	incubate	VBN
cord-104268-q1jx0n0l	88	47	for	for	IN
cord-104268-q1jx0n0l	88	48	10	10	CD
cord-104268-q1jx0n0l	88	49	min	min	NN
cord-104268-q1jx0n0l	88	50	at	at	IN
cord-104268-q1jx0n0l	88	51	30	30	CD
cord-104268-q1jx0n0l	88	52	°	°	NN
cord-104268-q1jx0n0l	88	53	C	c	NN
cord-104268-q1jx0n0l	88	54	with	with	IN
cord-104268-q1jx0n0l	88	55	gentle	gentle	JJ
cord-104268-q1jx0n0l	88	56	agitation	agitation	NN
cord-104268-q1jx0n0l	88	57	.	.	.
cord-104268-q1jx0n0l	89	1	After	after	IN
cord-104268-q1jx0n0l	89	2	washing	washing	NN
cord-104268-q1jx0n0l	89	3	of	of	IN
cord-104268-q1jx0n0l	89	4	the	the	DT
cord-104268-q1jx0n0l	89	5	fixed	fix	VBN
cord-104268-q1jx0n0l	89	6	cells	cell	NNS
cord-104268-q1jx0n0l	89	7	,	,	,
cord-104268-q1jx0n0l	89	8	cell	cell	NN
cord-104268-q1jx0n0l	89	9	walls	wall	NNS
cord-104268-q1jx0n0l	89	10	were	be	VBD
cord-104268-q1jx0n0l	89	11	removed	remove	VBN
cord-104268-q1jx0n0l	89	12	by	by	IN
cord-104268-q1jx0n0l	89	13	treatment	treatment	NN
cord-104268-q1jx0n0l	89	14	with	with	IN
cord-104268-q1jx0n0l	89	15	Zymolyase	Zymolyase	NNP
cord-104268-q1jx0n0l	89	16	100	100	CD
cord-104268-q1jx0n0l	89	17	T	t	NN
cord-104268-q1jx0n0l	89	18	(	(	-LRB-
cord-104268-q1jx0n0l	89	19	ICN	ICN	NNP
cord-104268-q1jx0n0l	89	20	Biochemicals	Biochemicals	NNPS
cord-104268-q1jx0n0l	89	21	)	)	-RRB-
cord-104268-q1jx0n0l	89	22	at	at	IN
cord-104268-q1jx0n0l	89	23	a	a	DT
cord-104268-q1jx0n0l	89	24	final	final	JJ
cord-104268-q1jx0n0l	89	25	concentration	concentration	NN
cord-104268-q1jx0n0l	89	26	of	of	IN
cord-104268-q1jx0n0l	89	27	200	200	CD
cord-104268-q1jx0n0l	89	28	ixg	ixg	NN
cord-104268-q1jx0n0l	90	1	/ml	/ml	NFP
cord-104268-q1jx0n0l	90	2	in	in	IN
cord-104268-q1jx0n0l	90	3	0.1	0.1	CD
cord-104268-q1jx0n0l	90	4	M	m	NN
cord-104268-q1jx0n0l	90	5	potassium	potassium	NN
cord-104268-q1jx0n0l	90	6	phosphate	phosphate	NN
cord-104268-q1jx0n0l	90	7	(	(	-LRB-
cord-104268-q1jx0n0l	90	8	pH	pH	NNP
cord-104268-q1jx0n0l	90	9	6.5	6.5	CD
cord-104268-q1jx0n0l	90	10	)	)	-RRB-
cord-104268-q1jx0n0l	90	11	1.2	1.2	CD
cord-104268-q1jx0n0l	90	12	M	m	CD
cord-104268-q1jx0n0l	90	13	sorbitol	sorbitol	NN
cord-104268-q1jx0n0l	90	14	for	for	IN
cord-104268-q1jx0n0l	90	15	20	20	CD
cord-104268-q1jx0n0l	90	16	-	-	SYM
cord-104268-q1jx0n0l	90	17	30	30	CD
cord-104268-q1jx0n0l	90	18	min	min	NN
cord-104268-q1jx0n0l	90	19	at	at	IN
cord-104268-q1jx0n0l	90	20	30	30	CD
cord-104268-q1jx0n0l	90	21	°	°	NN
cord-104268-q1jx0n0l	90	22	C	c	NN
cord-104268-q1jx0n0l	90	23	.	.	.
cord-104268-q1jx0n0l	91	1	Fixed	fix	VBN
cord-104268-q1jx0n0l	91	2	spheroplasted	spheroplasted	JJ
cord-104268-q1jx0n0l	91	3	cells	cell	NNS
cord-104268-q1jx0n0l	91	4	were	be	VBD
cord-104268-q1jx0n0l	91	5	washed	wash	VBN
cord-104268-q1jx0n0l	91	6	in	in	IN
cord-104268-q1jx0n0l	91	7	0.1	0.1	CD
cord-104268-q1jx0n0l	91	8	M	m	CD
cord-104268-q1jx0n0l	91	9	potassium	potassium	NN
cord-104268-q1jx0n0l	91	10	-	-	HYPH
cord-104268-q1jx0n0l	91	11	phosphate	phosphate	NN
cord-104268-q1jx0n0l	91	12	(	(	-LRB-
cord-104268-q1jx0n0l	91	13	pH	pH	NNP
cord-104268-q1jx0n0l	91	14	7.4	7.4	CD
cord-104268-q1jx0n0l	91	15	)	)	-RRB-
cord-104268-q1jx0n0l	91	16	1.2	1.2	CD
cord-104268-q1jx0n0l	91	17	M	m	CD
cord-104268-q1jx0n0l	91	18	sorbitol	sorbitol	NN
cord-104268-q1jx0n0l	91	19	and	and	CC
cord-104268-q1jx0n0l	91	20	resuspended	resuspend	VBD
cord-104268-q1jx0n0l	91	21	in	in	IN
cord-104268-q1jx0n0l	91	22	the	the	DT
cord-104268-q1jx0n0l	91	23	same	same	JJ
cord-104268-q1jx0n0l	91	24	buffer	buffer	NN
cord-104268-q1jx0n0l	91	25	.	.	.
cord-104268-q1jx0n0l	92	1	Cells	cell	NNS
cord-104268-q1jx0n0l	92	2	were	be	VBD
cord-104268-q1jx0n0l	92	3	subsequently	subsequently	RB
cord-104268-q1jx0n0l	92	4	adsorbed	adsorb	VBN
cord-104268-q1jx0n0l	92	5	to	to	IN
cord-104268-q1jx0n0l	92	6	poly	poly	NNP
cord-104268-q1jx0n0l	92	7	-	-	HYPH
cord-104268-q1jx0n0l	92	8	L	l	NN
cord-104268-q1jx0n0l	92	9	-	-	HYPH
cord-104268-q1jx0n0l	92	10	lysine	lysine	NN
cord-104268-q1jx0n0l	92	11	coated	coat	VBN
cord-104268-q1jx0n0l	92	12	microscope	microscope	NN
cord-104268-q1jx0n0l	92	13	slides	slide	NNS
cord-104268-q1jx0n0l	92	14	,	,	,
cord-104268-q1jx0n0l	92	15	permitted	permit	VBD
cord-104268-q1jx0n0l	92	16	to	to	TO
cord-104268-q1jx0n0l	92	17	stand	stand	VB
cord-104268-q1jx0n0l	92	18	for	for	IN
cord-104268-q1jx0n0l	92	19	10	10	CD
cord-104268-q1jx0n0l	92	20	min	min	NN
cord-104268-q1jx0n0l	92	21	and	and	CC
cord-104268-q1jx0n0l	92	22	washed	wash	VBD
cord-104268-q1jx0n0l	92	23	with	with	IN
cord-104268-q1jx0n0l	92	24	PBS	PBS	NNP
cord-104268-q1jx0n0l	92	25	.	.	.
cord-104268-q1jx0n0l	93	1	Slides	slide	NNS
cord-104268-q1jx0n0l	93	2	were	be	VBD
cord-104268-q1jx0n0l	93	3	then	then	RB
cord-104268-q1jx0n0l	93	4	immersed	immerse	VBN
cord-104268-q1jx0n0l	93	5	in	in	IN
cord-104268-q1jx0n0l	93	6	-20	-20	NNP
cord-104268-q1jx0n0l	93	7	°	°	NN
cord-104268-q1jx0n0l	93	8	C	c	NN
cord-104268-q1jx0n0l	93	9	methanol	methanol	NN
cord-104268-q1jx0n0l	93	10	for	for	IN
cord-104268-q1jx0n0l	93	11	1	1	CD
cord-104268-q1jx0n0l	93	12	-	-	SYM
cord-104268-q1jx0n0l	93	13	6	6	CD
cord-104268-q1jx0n0l	93	14	min	min	NN
cord-104268-q1jx0n0l	93	15	,	,	,
cord-104268-q1jx0n0l	93	16	and	and	CC
cord-104268-q1jx0n0l	93	17	then	then	RB
cord-104268-q1jx0n0l	93	18	for	for	IN
cord-104268-q1jx0n0l	93	19	20450	20450	CD
cord-104268-q1jx0n0l	93	20	s	s	NNS
cord-104268-q1jx0n0l	93	21	in	in	IN
cord-104268-q1jx0n0l	93	22	-20	-20	NNP
cord-104268-q1jx0n0l	93	23	°	°	NNP
cord-104268-q1jx0n0l	93	24	C	c	NN
cord-104268-q1jx0n0l	93	25	acetone	acetone	NN
cord-104268-q1jx0n0l	93	26	,	,	,
cord-104268-q1jx0n0l	93	27	depending	depend	VBG
cord-104268-q1jx0n0l	93	28	on	on	IN
cord-104268-q1jx0n0l	93	29	yeast	yeast	NN
cord-104268-q1jx0n0l	93	30	strains	strain	NNS
cord-104268-q1jx0n0l	93	31	.	.	.
cord-104268-q1jx0n0l	94	1	Treated	treat	VBN
cord-104268-q1jx0n0l	94	2	slides	slide	NNS
cord-104268-q1jx0n0l	94	3	were	be	VBD
cord-104268-q1jx0n0l	94	4	air	air	NN
cord-104268-q1jx0n0l	94	5	-	-	HYPH
cord-104268-q1jx0n0l	94	6	dried	dry	VBN
cord-104268-q1jx0n0l	94	7	and	and	CC
cord-104268-q1jx0n0l	94	8	used	use	VBN
cord-104268-q1jx0n0l	94	9	immediately	immediately	RB
cord-104268-q1jx0n0l	94	10	or	or	CC
cord-104268-q1jx0n0l	94	11	stored	store	VBN
cord-104268-q1jx0n0l	94	12	at	at	IN
cord-104268-q1jx0n0l	94	13	4	4	CD
cord-104268-q1jx0n0l	94	14	°	°	NN
cord-104268-q1jx0n0l	94	15	C	c	NN
cord-104268-q1jx0n0l	94	16	until	until	IN
cord-104268-q1jx0n0l	94	17	needed	need	VBN
cord-104268-q1jx0n0l	94	18	.	.	.
cord-104268-q1jx0n0l	95	1	Fixed	fix	VBN
cord-104268-q1jx0n0l	95	2	mounted	mount	VBN
cord-104268-q1jx0n0l	95	3	cells	cell	NNS
cord-104268-q1jx0n0l	95	4	were	be	VBD
cord-104268-q1jx0n0l	95	5	incubated	incubate	VBN
cord-104268-q1jx0n0l	95	6	with	with	IN
cord-104268-q1jx0n0l	95	7	primary	primary	JJ
cord-104268-q1jx0n0l	95	8	antibodies	antibody	NNS
cord-104268-q1jx0n0l	95	9	diluted	dilute	VBN
cord-104268-q1jx0n0l	95	10	in	in	IN
cord-104268-q1jx0n0l	95	11	PBS	PBS	NNP
cord-104268-q1jx0n0l	95	12	containing	contain	VBG
cord-104268-q1jx0n0l	95	13	0.5	0.5	CD
cord-104268-q1jx0n0l	95	14	mg	mg	NNP
cord-104268-q1jx0n0l	95	15	/	/	SYM
cord-104268-q1jx0n0l	95	16	ml	ml	NNP
cord-104268-q1jx0n0l	95	17	BSA	BSA	NNP
cord-104268-q1jx0n0l	95	18	for	for	IN
cord-104268-q1jx0n0l	95	19	1	1	CD
cord-104268-q1jx0n0l	95	20	h	h	NN
cord-104268-q1jx0n0l	95	21	at	at	IN
cord-104268-q1jx0n0l	95	22	25	25	CD
cord-104268-q1jx0n0l	95	23	°	°	NN
cord-104268-q1jx0n0l	95	24	C	c	NN
cord-104268-q1jx0n0l	95	25	or	or	CC
cord-104268-q1jx0n0l	95	26	overnight	overnight	RB
cord-104268-q1jx0n0l	95	27	at	at	IN
cord-104268-q1jx0n0l	95	28	4	4	CD
cord-104268-q1jx0n0l	95	29	°	°	NN
cord-104268-q1jx0n0l	95	30	C	c	NN
cord-104268-q1jx0n0l	95	31	.	.	.
cord-104268-q1jx0n0l	96	1	Anti	Anti	NNP
cord-104268-q1jx0n0l	96	2	-	-	NN
cord-104268-q1jx0n0l	96	3	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	96	4	Ab	Ab	NNP
cord-104268-q1jx0n0l	96	5	was	be	VBD
cord-104268-q1jx0n0l	96	6	used	use	VBN
cord-104268-q1jx0n0l	96	7	at	at	IN
cord-104268-q1jx0n0l	96	8	dilutions	dilution	NNS
cord-104268-q1jx0n0l	96	9	of	of	IN
cord-104268-q1jx0n0l	96	10	1:25	1:25	CD
cord-104268-q1jx0n0l	96	11	-	-	SYM
cord-104268-q1jx0n0l	96	12	1:100	1:100	CD
cord-104268-q1jx0n0l	96	13	.	.	.
cord-104268-q1jx0n0l	97	1	For	for	IN
cord-104268-q1jx0n0l	97	2	dual	dual	JJ
cord-104268-q1jx0n0l	97	3	-	-	HYPH
cord-104268-q1jx0n0l	97	4	labeling	label	VBG
cord-104268-q1jx0n0l	97	5	experiments	experiment	NNS
cord-104268-q1jx0n0l	97	6	involving	involve	VBG
cord-104268-q1jx0n0l	97	7	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	97	8	and	and	CC
cord-104268-q1jx0n0l	97	9	medialor	medialor	NN
cord-104268-q1jx0n0l	97	10	late	late	JJ
cord-104268-q1jx0n0l	97	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	97	12	markers	marker	NNS
cord-104268-q1jx0n0l	97	13	,	,	,
cord-104268-q1jx0n0l	97	14	the	the	DT
cord-104268-q1jx0n0l	97	15	influenza	influenza	NN
cord-104268-q1jx0n0l	97	16	hemagglutinin	hemagglutinin	NN
cord-104268-q1jx0n0l	97	17	virus	virus	NN
cord-104268-q1jx0n0l	97	18	epitope	epitope	NN
cord-104268-q1jx0n0l	97	19	(	(	-LRB-
cord-104268-q1jx0n0l	97	20	sequence	sequence	NN
cord-104268-q1jx0n0l	97	21	YPY	YPY	NNP
cord-104268-q1jx0n0l	97	22	-	-	HYPH
cord-104268-q1jx0n0l	97	23	DVPDYA	DVPDYA	NNP
cord-104268-q1jx0n0l	97	24	)	)	-RRB-
cord-104268-q1jx0n0l	97	25	was	be	VBD
cord-104268-q1jx0n0l	97	26	inserted	insert	VBN
cord-104268-q1jx0n0l	97	27	by	by	IN
cord-104268-q1jx0n0l	97	28	oligonucleotide	oligonucleotide	RB
cord-104268-q1jx0n0l	97	29	-	-	HYPH
cord-104268-q1jx0n0l	97	30	directed	direct	VBN
cord-104268-q1jx0n0l	97	31	mutagenesis	mutagenesis	NN
cord-104268-q1jx0n0l	97	32	directly	directly	RB
cord-104268-q1jx0n0l	97	33	at	at	IN
cord-104268-q1jx0n0l	97	34	the	the	DT
cord-104268-q1jx0n0l	97	35	COOH	COOH	NNP
cord-104268-q1jx0n0l	97	36	-	-	HYPH
cord-104268-q1jx0n0l	97	37	terminal	terminal	NN
cord-104268-q1jx0n0l	97	38	domain	domain	NN
cord-104268-q1jx0n0l	97	39	of	of	IN
cord-104268-q1jx0n0l	97	40	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	97	41	and	and	CC
cord-104268-q1jx0n0l	97	42	in	in	IN
cord-104268-q1jx0n0l	97	43	the	the	DT
cord-104268-q1jx0n0l	97	44	region	region	NN
cord-104268-q1jx0n0l	97	45	corresponding	correspond	VBG
cord-104268-q1jx0n0l	97	46	to	to	IN
cord-104268-q1jx0n0l	97	47	the	the	DT
cord-104268-q1jx0n0l	97	48	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	97	49	luminal	luminal	JJ
cord-104268-q1jx0n0l	97	50	domain	domain	NN
cord-104268-q1jx0n0l	97	51	.	.	.
cord-104268-q1jx0n0l	98	1	Epitope	Epitope	NNP
cord-104268-q1jx0n0l	98	2	-	-	HYPH
cord-104268-q1jx0n0l	98	3	tagged	tagged	JJ
cord-104268-q1jx0n0l	98	4	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	98	5	and	and	CC
cord-104268-q1jx0n0l	98	6	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	98	7	were	be	VBD
cord-104268-q1jx0n0l	98	8	detected	detect	VBN
cord-104268-q1jx0n0l	98	9	with	with	IN
cord-104268-q1jx0n0l	98	10	the	the	DT
cord-104268-q1jx0n0l	98	11	12CA5	12ca5	CD
cord-104268-q1jx0n0l	98	12	monoclonal	monoclonal	JJ
cord-104268-q1jx0n0l	98	13	antibody	antibody	NN
cord-104268-q1jx0n0l	98	14	(	(	-LRB-
cord-104268-q1jx0n0l	98	15	Kolodziej	Kolodziej	NNP
cord-104268-q1jx0n0l	98	16	and	and	CC
cord-104268-q1jx0n0l	98	17	Young	Young	NNP
cord-104268-q1jx0n0l	98	18	,	,	,
cord-104268-q1jx0n0l	98	19	1991	1991	CD
cord-104268-q1jx0n0l	98	20	)	)	-RRB-
cord-104268-q1jx0n0l	98	21	.	.	.
cord-104268-q1jx0n0l	99	1	The	the	DT
cord-104268-q1jx0n0l	99	2	latter	latter	JJ
cord-104268-q1jx0n0l	99	3	was	be	VBD
cord-104268-q1jx0n0l	99	4	used	use	VBN
cord-104268-q1jx0n0l	99	5	at	at	IN
cord-104268-q1jx0n0l	99	6	dilutions	dilution	NNS
cord-104268-q1jx0n0l	99	7	ranging	range	VBG
cord-104268-q1jx0n0l	99	8	from	from	IN
cord-104268-q1jx0n0l	99	9	1:250	1:250	CD
cord-104268-q1jx0n0l	99	10	-	-	HYPH
cord-104268-q1jx0n0l	99	11	-1:1,000	-1:1,000	NNS
cord-104268-q1jx0n0l	99	12	.	.	.
cord-104268-q1jx0n0l	100	1	mAb	mAb	NNS
cord-104268-q1jx0n0l	100	2	13Dll	13dll	CD
cord-104268-q1jx0n0l	100	3	which	which	WDT
cord-104268-q1jx0n0l	100	4	recognizes	recognize	VBZ
cord-104268-q1jx0n0l	100	5	the	the	DT
cord-104268-q1jx0n0l	100	6	60-kD	60-kd	CD
cord-104268-q1jx0n0l	100	7	subunit	subunit	NN
cord-104268-q1jx0n0l	100	8	of	of	IN
cord-104268-q1jx0n0l	100	9	the	the	DT
cord-104268-q1jx0n0l	100	10	yeast	yeast	NN
cord-104268-q1jx0n0l	100	11	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	100	12	membrane	membrane	NN
cord-104268-q1jx0n0l	100	13	H+-ATPase	h+-atpase	NN
cord-104268-q1jx0n0l	101	1	(	(	-LRB-
cord-104268-q1jx0n0l	101	2	Kane	Kane	NNP
cord-104268-q1jx0n0l	101	3	et	et	NNP
cord-104268-q1jx0n0l	101	4	al	al	NNP
cord-104268-q1jx0n0l	101	5	.	.	NNP
cord-104268-q1jx0n0l	101	6	,	,	,
cord-104268-q1jx0n0l	101	7	1992	1992	CD
cord-104268-q1jx0n0l	101	8	)	)	-RRB-
cord-104268-q1jx0n0l	101	9	was	be	VBD
cord-104268-q1jx0n0l	101	10	used	use	VBN
cord-104268-q1jx0n0l	101	11	at	at	IN
cord-104268-q1jx0n0l	101	12	dilutions	dilution	NNS
cord-104268-q1jx0n0l	101	13	of	of	IN
cord-104268-q1jx0n0l	101	14	1:10	1:10	CD
cord-104268-q1jx0n0l	101	15	-	-	SYM
cord-104268-q1jx0n0l	101	16	1:25	1:25	CD
cord-104268-q1jx0n0l	101	17	as	as	IN
cord-104268-q1jx0n0l	101	18	a	a	DT
cord-104268-q1jx0n0l	101	19	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	101	20	marker	marker	NN
cord-104268-q1jx0n0l	101	21	for	for	IN
cord-104268-q1jx0n0l	101	22	colocalization	colocalization	NN
cord-104268-q1jx0n0l	101	23	studies	study	NNS
cord-104268-q1jx0n0l	101	24	with	with	IN
cord-104268-q1jx0n0l	101	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	101	26	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	101	27	proteins	protein	NNS
cord-104268-q1jx0n0l	101	28	.	.	.
cord-104268-q1jx0n0l	102	1	Fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	102	2	signals	signal	NNS
cord-104268-q1jx0n0l	102	3	were	be	VBD
cord-104268-q1jx0n0l	102	4	obtained	obtain	VBN
cord-104268-q1jx0n0l	102	5	by	by	IN
cord-104268-q1jx0n0l	102	6	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	102	7	incubation	incubation	NN
cord-104268-q1jx0n0l	102	8	of	of	IN
cord-104268-q1jx0n0l	102	9	treated	treat	VBN
cord-104268-q1jx0n0l	102	10	cells	cell	NNS
cord-104268-q1jx0n0l	102	11	with	with	IN
cord-104268-q1jx0n0l	102	12	rhodamine	rhodamine	NN
cord-104268-q1jx0n0l	102	13	X	x	NN
cord-104268-q1jx0n0l	102	14	sulfonyl	sulfonyl	NN
cord-104268-q1jx0n0l	102	15	chloride	chloride	NN
cord-104268-q1jx0n0l	103	1	(	(	-LRB-
cord-104268-q1jx0n0l	103	2	Texas	Texas	NNP
cord-104268-q1jx0n0l	103	3	red)-conjugated	red)-conjugated	NNP
cord-104268-q1jx0n0l	103	4	goat	goat	NN
cord-104268-q1jx0n0l	103	5	anti	anti	JJ
cord-104268-q1jx0n0l	103	6	-	-	JJ
cord-104268-q1jx0n0l	103	7	rabbit	rabbit	JJ
cord-104268-q1jx0n0l	103	8	IgG	igg	NN
cord-104268-q1jx0n0l	103	9	(	(	-LRB-
cord-104268-q1jx0n0l	103	10	1:50	1:50	CD
cord-104268-q1jx0n0l	103	11	-	-	HYPH
cord-104268-q1jx0n0l	103	12	1:200	1:200	CD
cord-104268-q1jx0n0l	103	13	)	)	-RRB-
cord-104268-q1jx0n0l	103	14	and	and	CC
cord-104268-q1jx0n0l	103	15	FfTC	FfTC	NNP
cord-104268-q1jx0n0l	103	16	-	-	HYPH
cord-104268-q1jx0n0l	103	17	conjugated	conjugate	VBN
cord-104268-q1jx0n0l	103	18	goat	goat	NN
cord-104268-q1jx0n0l	103	19	anti	anti	JJ
cord-104268-q1jx0n0l	103	20	-	-	JJ
cord-104268-q1jx0n0l	103	21	mouse	mouse	JJ
cord-104268-q1jx0n0l	103	22	IgG	igg	NN
cord-104268-q1jx0n0l	103	23	(	(	-LRB-
cord-104268-q1jx0n0l	103	24	1:50	1:50	CD
cord-104268-q1jx0n0l	103	25	-	-	HYPH
cord-104268-q1jx0n0l	103	26	1:200	1:200	NNP
cord-104268-q1jx0n0l	103	27	)	)	-RRB-
cord-104268-q1jx0n0l	103	28	which	which	WDT
cord-104268-q1jx0n0l	103	29	were	be	VBD
cord-104268-q1jx0n0l	103	30	used	use	VBN
cord-104268-q1jx0n0l	103	31	as	as	IN
cord-104268-q1jx0n0l	103	32	secondary	secondary	JJ
cord-104268-q1jx0n0l	103	33	antibodies	antibody	NNS
cord-104268-q1jx0n0l	103	34	.	.	.
cord-104268-q1jx0n0l	104	1	Nuclei	nucleus	NNS
cord-104268-q1jx0n0l	104	2	and	and	CC
cord-104268-q1jx0n0l	104	3	mitochondria	mitochondrion	NNS
cord-104268-q1jx0n0l	104	4	were	be	VBD
cord-104268-q1jx0n0l	104	5	visualized	visualize	VBN
cord-104268-q1jx0n0l	104	6	by	by	IN
cord-104268-q1jx0n0l	104	7	staining	stain	VBG
cord-104268-q1jx0n0l	104	8	with	with	IN
cord-104268-q1jx0n0l	104	9	4',6-diamidino-2-phenyl	4',6-diamidino-2-phenyl	CD
cord-104268-q1jx0n0l	104	10	-	-	HYPH
cord-104268-q1jx0n0l	104	11	indole	indole	NN
cord-104268-q1jx0n0l	104	12	(	(	-LRB-
cord-104268-q1jx0n0l	104	13	DAPI	DAPI	NNP
cord-104268-q1jx0n0l	104	14	)	)	-RRB-
cord-104268-q1jx0n0l	104	15	.	.	.
cord-104268-q1jx0n0l	105	1	Cells	cell	NNS
cord-104268-q1jx0n0l	105	2	were	be	VBD
cord-104268-q1jx0n0l	105	3	examined	examine	VBN
cord-104268-q1jx0n0l	105	4	with	with	IN
cord-104268-q1jx0n0l	105	5	an	an	DT
cord-104268-q1jx0n0l	105	6	epifluorescence	epifluorescence	NN
cord-104268-q1jx0n0l	105	7	microscope	microscope	NN
cord-104268-q1jx0n0l	105	8	(	(	-LRB-
cord-104268-q1jx0n0l	105	9	Axiophot	Axiophot	NNP
cord-104268-q1jx0n0l	105	10	;	;	:
cord-104268-q1jx0n0l	105	11	Carl	Carl	NNP
cord-104268-q1jx0n0l	105	12	Zeiss	Zeiss	NNP
cord-104268-q1jx0n0l	105	13	,	,	,
cord-104268-q1jx0n0l	105	14	Inc.	Inc.	NNP
cord-104268-q1jx0n0l	105	15	,	,	,
cord-104268-q1jx0n0l	105	16	Thornwood	Thornwood	NNP
cord-104268-q1jx0n0l	105	17	,	,	,
cord-104268-q1jx0n0l	105	18	NY	NY	NNP
cord-104268-q1jx0n0l	105	19	)	)	-RRB-
cord-104268-q1jx0n0l	105	20	,	,	,
cord-104268-q1jx0n0l	105	21	and	and	CC
cord-104268-q1jx0n0l	105	22	photographed	photograph	VBN
cord-104268-q1jx0n0l	105	23	with	with	IN
cord-104268-q1jx0n0l	105	24	film	film	NN
cord-104268-q1jx0n0l	105	25	(	(	-LRB-
cord-104268-q1jx0n0l	105	26	T	T	NNP
cord-104268-q1jx0n0l	105	27	-	-	HYPH
cord-104268-q1jx0n0l	105	28	Max	Max	NNP
cord-104268-q1jx0n0l	105	29	400	400	CD
cord-104268-q1jx0n0l	105	30	;	;	:
cord-104268-q1jx0n0l	105	31	Eastman	Eastman	NNP
cord-104268-q1jx0n0l	105	32	Kodak	Kodak	NNP
cord-104268-q1jx0n0l	105	33	Co.	Co.	NNP
cord-104268-q1jx0n0l	105	34	,	,	,
cord-104268-q1jx0n0l	105	35	Rochester	Rochester	NNP
cord-104268-q1jx0n0l	105	36	,	,	,
cord-104268-q1jx0n0l	105	37	NY	NY	NNP
cord-104268-q1jx0n0l	105	38	)	)	-RRB-
cord-104268-q1jx0n0l	105	39	.	.	.
cord-104268-q1jx0n0l	106	1	The	the	DT
cord-104268-q1jx0n0l	106	2	extent	extent	NN
cord-104268-q1jx0n0l	106	3	of	of	IN
cord-104268-q1jx0n0l	106	4	colocalization	colocalization	NN
cord-104268-q1jx0n0l	106	5	of	of	IN
cord-104268-q1jx0n0l	106	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	106	7	with	with	IN
cord-104268-q1jx0n0l	106	8	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	106	9	or	or	CC
cord-104268-q1jx0n0l	106	10	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	106	11	was	be	VBD
cord-104268-q1jx0n0l	106	12	scored	score	VBN
cord-104268-q1jx0n0l	106	13	by	by	IN
cord-104268-q1jx0n0l	106	14	quantitating	quantitate	VBG
cord-104268-q1jx0n0l	106	15	in	in	IN
cord-104268-q1jx0n0l	106	16	a	a	DT
cord-104268-q1jx0n0l	106	17	given	give	VBN
cord-104268-q1jx0n0l	106	18	dual	dual	JJ
cord-104268-q1jx0n0l	106	19	localization	localization	NN
cord-104268-q1jx0n0l	106	20	experiment	experiment	NN
cord-104268-q1jx0n0l	106	21	30	30	CD
cord-104268-q1jx0n0l	106	22	cells	cell	NNS
cord-104268-q1jx0n0l	106	23	that	that	WDT
cord-104268-q1jx0n0l	106	24	contained	contain	VBD
cord-104268-q1jx0n0l	106	25	clearly	clearly	RB
cord-104268-q1jx0n0l	106	26	defined	define	VBN
cord-104268-q1jx0n0l	106	27	signals	signal	NNS
cord-104268-q1jx0n0l	106	28	representing	represent	VBG
cord-104268-q1jx0n0l	106	29	200	200	CD
cord-104268-q1jx0n0l	106	30	-	-	SYM
cord-104268-q1jx0n0l	106	31	250	250	CD
cord-104268-q1jx0n0l	106	32	individual	individual	JJ
cord-104268-q1jx0n0l	106	33	fluorescent	fluorescent	NN
cord-104268-q1jx0n0l	106	34	punctate	punctate	NN
cord-104268-q1jx0n0l	106	35	spots	spot	NNS
cord-104268-q1jx0n0l	106	36	for	for	IN
cord-104268-q1jx0n0l	106	37	each	each	DT
cord-104268-q1jx0n0l	106	38	antigen	antigen	NN
cord-104268-q1jx0n0l	106	39	.	.	.
cord-104268-q1jx0n0l	107	1	Compiled	compile	VBN
cord-104268-q1jx0n0l	107	2	data	datum	NNS
cord-104268-q1jx0n0l	107	3	revealed	reveal	VBD
cord-104268-q1jx0n0l	107	4	that	that	IN
cord-104268-q1jx0n0l	107	5	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	107	6	gave	give	VBD
cord-104268-q1jx0n0l	107	7	rise	rise	NN
cord-104268-q1jx0n0l	107	8	to	to	IN
cord-104268-q1jx0n0l	107	9	approximately	approximately	RB
cord-104268-q1jx0n0l	107	10	the	the	DT
cord-104268-q1jx0n0l	107	11	same	same	JJ
cord-104268-q1jx0n0l	107	12	number	number	NN
cord-104268-q1jx0n0l	107	13	of	of	IN
cord-104268-q1jx0n0l	107	14	punctate	punctate	NN
cord-104268-q1jx0n0l	107	15	spots	spot	NNS
cord-104268-q1jx0n0l	107	16	per	per	IN
cord-104268-q1jx0n0l	107	17	cell	cell	NN
cord-104268-q1jx0n0l	107	18	as	as	IN
cord-104268-q1jx0n0l	107	19	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	107	20	and	and	CC
cord-104268-q1jx0n0l	107	21	75	75	CD
cord-104268-q1jx0n0l	107	22	%	%	NN
cord-104268-q1jx0n0l	107	23	of	of	IN
cord-104268-q1jx0n0l	107	24	the	the	DT
cord-104268-q1jx0n0l	107	25	punctiform	punctiform	NNP
cord-104268-q1jx0n0l	107	26	fluorescent	fluorescent	NN
cord-104268-q1jx0n0l	107	27	spots	spot	NNS
cord-104268-q1jx0n0l	107	28	from	from	IN
cord-104268-q1jx0n0l	107	29	Texas	Texas	NNP
cord-104268-q1jx0n0l	107	30	red	red	NN
cord-104268-q1jx0n0l	107	31	and	and	CC
cord-104268-q1jx0n0l	107	32	FITC	FITC	NNP
cord-104268-q1jx0n0l	107	33	overlapped	overlap	VBD
cord-104268-q1jx0n0l	107	34	.	.	.
cord-104268-q1jx0n0l	108	1	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	108	2	gave	give	VBD
cord-104268-q1jx0n0l	108	3	rise	rise	NN
cord-104268-q1jx0n0l	108	4	to	to	IN
cord-104268-q1jx0n0l	108	5	~10	~10	CD
cord-104268-q1jx0n0l	108	6	%	%	NN
cord-104268-q1jx0n0l	108	7	fewer	few	JJR
cord-104268-q1jx0n0l	108	8	punctate	punctate	NN
cord-104268-q1jx0n0l	108	9	spots	spot	NNS
cord-104268-q1jx0n0l	108	10	per	per	IN
cord-104268-q1jx0n0l	108	11	cell	cell	NN
cord-104268-q1jx0n0l	108	12	compared	compare	VBN
cord-104268-q1jx0n0l	108	13	with	with	IN
cord-104268-q1jx0n0l	108	14	Kre2p	kre2p	NN
cord-104268-q1jx0n0l	108	15	-	-	HYPH
cord-104268-q1jx0n0l	108	16	associated	associate	VBN
cord-104268-q1jx0n0l	108	17	signals	signal	NNS
cord-104268-q1jx0n0l	108	18	,	,	,
cord-104268-q1jx0n0l	108	19	and	and	CC
cord-104268-q1jx0n0l	108	20	65	65	CD
cord-104268-q1jx0n0l	108	21	%	%	NN
cord-104268-q1jx0n0l	108	22	of	of	IN
cord-104268-q1jx0n0l	108	23	the	the	DT
cord-104268-q1jx0n0l	108	24	punctiform	punctiform	NNP
cord-104268-q1jx0n0l	108	25	fluorescent	fluorescent	NN
cord-104268-q1jx0n0l	108	26	spots	spot	NNS
cord-104268-q1jx0n0l	108	27	emanating	emanate	VBG
cord-104268-q1jx0n0l	108	28	from	from	IN
cord-104268-q1jx0n0l	108	29	both	both	DT
cord-104268-q1jx0n0l	108	30	proteins	protein	NNS
cord-104268-q1jx0n0l	108	31	did	do	VBD
cord-104268-q1jx0n0l	108	32	not	not	RB
cord-104268-q1jx0n0l	108	33	overlap	overlap	VB
cord-104268-q1jx0n0l	108	34	.	.	.
cord-104268-q1jx0n0l	109	1	Finally	finally	RB
cord-104268-q1jx0n0l	109	2	,	,	,
cord-104268-q1jx0n0l	109	3	the	the	DT
cord-104268-q1jx0n0l	109	4	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	109	5	localization	localization	NN
cord-104268-q1jx0n0l	109	6	of	of	IN
cord-104268-q1jx0n0l	109	7	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	109	8	protein	protein	NN
cord-104268-q1jx0n0l	109	9	KKP	KKP	NNP
cord-104268-q1jx0n0l	109	10	was	be	VBD
cord-104268-q1jx0n0l	109	11	quantitatively	quantitatively	RB
cord-104268-q1jx0n0l	109	12	scored	score	VBN
cord-104268-q1jx0n0l	109	13	by	by	IN
cord-104268-q1jx0n0l	109	14	examining	examine	VBG
cord-104268-q1jx0n0l	109	15	750	750	CD
cord-104268-q1jx0n0l	109	16	individual	individual	JJ
cord-104268-q1jx0n0l	109	17	ceils	ceil	NNS
cord-104268-q1jx0n0l	109	18	containing	contain	VBG
cord-104268-q1jx0n0l	109	19	clearly	clearly	RB
cord-104268-q1jx0n0l	109	20	defined	define	VBN
cord-104268-q1jx0n0l	109	21	signals	signal	NNS
cord-104268-q1jx0n0l	109	22	.	.	.
cord-104268-q1jx0n0l	110	1	Punctiform	punctiform	NN
cord-104268-q1jx0n0l	110	2	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	110	3	different	different	JJ
cord-104268-q1jx0n0l	110	4	from	from	IN
cord-104268-q1jx0n0l	110	5	nucleus	nucleus	NN
cord-104268-q1jx0n0l	110	6	,	,	,
cord-104268-q1jx0n0l	110	7	ER	ER	NNP
cord-104268-q1jx0n0l	110	8	,	,	,
cord-104268-q1jx0n0l	110	9	or	or	CC
cord-104268-q1jx0n0l	110	10	vacuole	vacuole	NN
cord-104268-q1jx0n0l	110	11	was	be	VBD
cord-104268-q1jx0n0l	110	12	defined	define	VBN
cord-104268-q1jx0n0l	110	13	as	as	IN
cord-104268-q1jx0n0l	110	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	110	15	localization	localization	NN
cord-104268-q1jx0n0l	110	16	.	.	.
cord-104268-q1jx0n0l	111	1	Vacuolar	Vacuolar	NNP
cord-104268-q1jx0n0l	111	2	localization	localization	NN
cord-104268-q1jx0n0l	111	3	was	be	VBD
cord-104268-q1jx0n0l	111	4	determined	determine	VBN
cord-104268-q1jx0n0l	111	5	by	by	IN
cord-104268-q1jx0n0l	111	6	colocalization	colocalization	NN
cord-104268-q1jx0n0l	111	7	with	with	IN
cord-104268-q1jx0n0l	111	8	the	the	DT
cord-104268-q1jx0n0l	111	9	60-kD	60-kd	CD
cord-104268-q1jx0n0l	111	10	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	111	11	membrane	membrane	NN
cord-104268-q1jx0n0l	111	12	H+-ATPase	h+-atpase	NN
cord-104268-q1jx0n0l	111	13	subunit	subunit	NN
cord-104268-q1jx0n0l	111	14	.	.	.
cord-104268-q1jx0n0l	112	1	The	the	DT
cord-104268-q1jx0n0l	112	2	product	product	NN
cord-104268-q1jx0n0l	112	3	encoded	encode	VBN
cord-104268-q1jx0n0l	112	4	by	by	IN
cord-104268-q1jx0n0l	112	5	the	the	DT
cord-104268-q1jx0n0l	112	6	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	112	7	gene	gene	NN
cord-104268-q1jx0n0l	112	8	is	be	VBZ
cord-104268-q1jx0n0l	112	9	a	a	DT
cord-104268-q1jx0n0l	112	10	predicted	predict	VBN
cord-104268-q1jx0n0l	112	11	type	type	NN
cord-104268-q1jx0n0l	112	12	II	II	NNP
cord-104268-q1jx0n0l	112	13	membrane	membrane	NN
cord-104268-q1jx0n0l	112	14	protein	protein	NN
cord-104268-q1jx0n0l	112	15	of	of	IN
cord-104268-q1jx0n0l	112	16	442	442	CD
cord-104268-q1jx0n0l	112	17	amino	amino	NN
cord-104268-q1jx0n0l	112	18	acid	acid	NN
cord-104268-q1jx0n0l	112	19	residues	residue	NNS
cord-104268-q1jx0n0l	112	20	with	with	IN
cord-104268-q1jx0n0l	112	21	a	a	DT
cord-104268-q1jx0n0l	112	22	short	short	JJ
cord-104268-q1jx0n0l	112	23	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	112	24	NH2	NH2	NNP
cord-104268-q1jx0n0l	112	25	terminus	terminus	NN
cord-104268-q1jx0n0l	112	26	,	,	,
cord-104268-q1jx0n0l	112	27	a	a	DT
cord-104268-q1jx0n0l	112	28	hydrophobic	hydrophobic	JJ
cord-104268-q1jx0n0l	112	29	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	112	30	region	region	NN
cord-104268-q1jx0n0l	112	31	,	,	,
cord-104268-q1jx0n0l	112	32	and	and	CC
cord-104268-q1jx0n0l	112	33	a	a	DT
cord-104268-q1jx0n0l	112	34	large	large	JJ
cord-104268-q1jx0n0l	112	35	luminal	luminal	JJ
cord-104268-q1jx0n0l	112	36	enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	112	37	domain	domain	NN
cord-104268-q1jx0n0l	112	38	containing	contain	VBG
cord-104268-q1jx0n0l	112	39	one	one	CD
cord-104268-q1jx0n0l	112	40	potential	potential	JJ
cord-104268-q1jx0n0l	112	41	N	n	CD
cord-104268-q1jx0n0l	112	42	-	-	HYPH
cord-104268-q1jx0n0l	112	43	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	112	44	site	site	NN
cord-104268-q1jx0n0l	112	45	(	(	-LRB-
cord-104268-q1jx0n0l	112	46	Fig	Fig	NNP
cord-104268-q1jx0n0l	112	47	.	.	.
cord-104268-q1jx0n0l	112	48	1	1	CD
cord-104268-q1jx0n0l	112	49	A	a	CD
cord-104268-q1jx0n0l	112	50	)	)	-RRB-
cord-104268-q1jx0n0l	112	51	.	.	.
cord-104268-q1jx0n0l	113	1	To	to	TO
cord-104268-q1jx0n0l	113	2	identify	identify	VB
cord-104268-q1jx0n0l	113	3	and	and	CC
cord-104268-q1jx0n0l	113	4	subsequently	subsequently	RB
cord-104268-q1jx0n0l	113	5	analyze	analyze	VB
cord-104268-q1jx0n0l	113	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	113	7	,	,	,
cord-104268-q1jx0n0l	113	8	a	a	DT
cord-104268-q1jx0n0l	113	9	specific	specific	JJ
cord-104268-q1jx0n0l	113	10	rabbit	rabbit	NN
cord-104268-q1jx0n0l	113	11	antiserum	antiserum	NN
cord-104268-q1jx0n0l	113	12	was	be	VBD
cord-104268-q1jx0n0l	113	13	raised	raise	VBN
cord-104268-q1jx0n0l	113	14	,	,	,
cord-104268-q1jx0n0l	113	15	affinity	affinity	NN
cord-104268-q1jx0n0l	113	16	purified	purify	VBN
cord-104268-q1jx0n0l	113	17	,	,	,
cord-104268-q1jx0n0l	113	18	and	and	CC
cord-104268-q1jx0n0l	113	19	used	use	VBD
cord-104268-q1jx0n0l	113	20	to	to	TO
cord-104268-q1jx0n0l	113	21	detect	detect	VB
cord-104268-q1jx0n0l	113	22	antigen	antigen	NN
cord-104268-q1jx0n0l	113	23	-	-	HYPH
cord-104268-q1jx0n0l	113	24	antibody	antibody	NN
cord-104268-q1jx0n0l	113	25	complexes	complex	NNS
cord-104268-q1jx0n0l	113	26	by	by	IN
cord-104268-q1jx0n0l	113	27	Western	western	JJ
cord-104268-q1jx0n0l	113	28	blotting	blotting	NN
cord-104268-q1jx0n0l	113	29	of	of	IN
cord-104268-q1jx0n0l	113	30	total	total	JJ
cord-104268-q1jx0n0l	113	31	-	-	HYPH
cord-104268-q1jx0n0l	113	32	cell	cell	NN
cord-104268-q1jx0n0l	113	33	protein	protein	NN
cord-104268-q1jx0n0l	113	34	extracts	extract	NNS
cord-104268-q1jx0n0l	113	35	of	of	IN
cord-104268-q1jx0n0l	113	36	yeast	yeast	NN
cord-104268-q1jx0n0l	113	37	strains	strain	NNS
cord-104268-q1jx0n0l	113	38	harboring	harbor	VBG
cord-104268-q1jx0n0l	113	39	a	a	DT
cord-104268-q1jx0n0l	113	40	KRE2	kre2	JJ
cord-104268-q1jx0n0l	113	41	disruption	disruption	NN
cord-104268-q1jx0n0l	113	42	or	or	CC
cord-104268-q1jx0n0l	113	43	containing	contain	VBG
cord-104268-q1jx0n0l	113	44	a	a	DT
cord-104268-q1jx0n0l	113	45	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	113	46	multicopy	multicopy	NN
cord-104268-q1jx0n0l	113	47	plasmid	plasmid	NN
cord-104268-q1jx0n0l	113	48	to	to	TO
cord-104268-q1jx0n0l	113	49	facilitate	facilitate	VB
cord-104268-q1jx0n0l	113	50	immunological	immunological	JJ
cord-104268-q1jx0n0l	113	51	detection	detection	NN
cord-104268-q1jx0n0l	113	52	.	.	.
cord-104268-q1jx0n0l	114	1	The	the	DT
cord-104268-q1jx0n0l	114	2	affinity	affinity	NN
cord-104268-q1jx0n0l	114	3	-	-	HYPH
cord-104268-q1jx0n0l	114	4	purified	purify	VBN
cord-104268-q1jx0n0l	114	5	Ab	Ab	NNP
cord-104268-q1jx0n0l	114	6	detected	detect	VBD
cord-104268-q1jx0n0l	114	7	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	114	8	as	as	IN
cord-104268-q1jx0n0l	114	9	a	a	DT
cord-104268-q1jx0n0l	114	10	59	59	CD
cord-104268-q1jx0n0l	114	11	-	-	SYM
cord-104268-q1jx0n0l	114	12	60	60	CD
cord-104268-q1jx0n0l	114	13	kD	kd	JJ
cord-104268-q1jx0n0l	114	14	protein	protein	NN
cord-104268-q1jx0n0l	114	15	in	in	IN
cord-104268-q1jx0n0l	114	16	the	the	DT
cord-104268-q1jx0n0l	114	17	KRE2overexpressing	KRE2overexpressing	NNP
cord-104268-q1jx0n0l	114	18	strain	strain	NN
cord-104268-q1jx0n0l	114	19	that	that	WDT
cord-104268-q1jx0n0l	114	20	was	be	VBD
cord-104268-q1jx0n0l	114	21	absent	absent	JJ
cord-104268-q1jx0n0l	114	22	from	from	IN
cord-104268-q1jx0n0l	114	23	the	the	DT
cord-104268-q1jx0n0l	114	24	kre2::TRP1	kre2::TRP1	NNP
cord-104268-q1jx0n0l	114	25	strain	strain	NN
cord-104268-q1jx0n0l	114	26	(	(	-LRB-
cord-104268-q1jx0n0l	114	27	Fig	fig	NN
cord-104268-q1jx0n0l	114	28	.	.	.
cord-104268-q1jx0n0l	114	29	1	1	CD
cord-104268-q1jx0n0l	114	30	B	b	NN
cord-104268-q1jx0n0l	114	31	)	)	-RRB-
cord-104268-q1jx0n0l	114	32	,	,	,
cord-104268-q1jx0n0l	114	33	and	and	CC
cord-104268-q1jx0n0l	114	34	not	not	RB
cord-104268-q1jx0n0l	114	35	detected	detect	VBN
cord-104268-q1jx0n0l	114	36	by	by	IN
cord-104268-q1jx0n0l	114	37	preimmune	preimmune	NNP
cord-104268-q1jx0n0l	114	38	antiserum	antiserum	NNP
cord-104268-q1jx0n0l	114	39	(	(	-LRB-
cord-104268-q1jx0n0l	114	40	data	datum	NNS
cord-104268-q1jx0n0l	114	41	not	not	RB
cord-104268-q1jx0n0l	114	42	shown	show	VBN
cord-104268-q1jx0n0l	114	43	)	)	-RRB-
cord-104268-q1jx0n0l	114	44	.	.	.
cord-104268-q1jx0n0l	115	1	The	the	DT
cord-104268-q1jx0n0l	115	2	in	in	IN
cord-104268-q1jx0n0l	115	3	vivo	vivo	NN
cord-104268-q1jx0n0l	115	4	-	-	HYPH
cord-104268-q1jx0n0l	115	5	produced	produce	VBN
cord-104268-q1jx0n0l	115	6	Kre2p	kre2p	NN
cord-104268-q1jx0n0l	115	7	is	be	VBZ
cord-104268-q1jx0n0l	115	8	~8.0	~8.0	CD
cord-104268-q1jx0n0l	115	9	kD	kd	ADD
cord-104268-q1jx0n0l	115	10	larger	large	JJR
cord-104268-q1jx0n0l	115	11	than	than	IN
cord-104268-q1jx0n0l	115	12	the	the	DT
cord-104268-q1jx0n0l	115	13	51.5-kD	51.5-kD	NNP
cord-104268-q1jx0n0l	115	14	molecular	molecular	JJ
cord-104268-q1jx0n0l	115	15	mass	mass	NN
cord-104268-q1jx0n0l	115	16	predicted	predict	VBN
cord-104268-q1jx0n0l	115	17	from	from	IN
cord-104268-q1jx0n0l	115	18	the	the	DT
cord-104268-q1jx0n0l	115	19	D	D	NNP
cord-104268-q1jx0n0l	115	20	N	n	NN
cord-104268-q1jx0n0l	115	21	A	a	DT
cord-104268-q1jx0n0l	115	22	sequence	sequence	NN
cord-104268-q1jx0n0l	115	23	.	.	.
cord-104268-q1jx0n0l	116	1	The	the	DT
cord-104268-q1jx0n0l	116	2	possible	possible	JJ
cord-104268-q1jx0n0l	116	3	integral	integral	JJ
cord-104268-q1jx0n0l	116	4	membrane	membrane	NN
cord-104268-q1jx0n0l	116	5	protein	protein	NN
cord-104268-q1jx0n0l	116	6	nature	nature	NN
cord-104268-q1jx0n0l	116	7	of	of	IN
cord-104268-q1jx0n0l	116	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	116	9	was	be	VBD
cord-104268-q1jx0n0l	116	10	examined	examine	VBN
cord-104268-q1jx0n0l	116	11	by	by	IN
cord-104268-q1jx0n0l	116	12	using	use	VBG
cord-104268-q1jx0n0l	116	13	reagents	reagent	NNS
cord-104268-q1jx0n0l	116	14	extracting	extract	VBG
cord-104268-q1jx0n0l	116	15	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	116	16	,	,	,
cord-104268-q1jx0n0l	116	17	vesicle	vesicle	NN
cord-104268-q1jx0n0l	116	18	-	-	HYPH
cord-104268-q1jx0n0l	116	19	enclosed	enclose	VBN
cord-104268-q1jx0n0l	116	20	,	,	,
cord-104268-q1jx0n0l	116	21	and	and	CC
cord-104268-q1jx0n0l	116	22	peripheral	peripheral	JJ
cord-104268-q1jx0n0l	116	23	membrane	membrane	NN
cord-104268-q1jx0n0l	116	24	proteins	protein	NNS
cord-104268-q1jx0n0l	116	25	but	but	CC
cord-104268-q1jx0n0l	116	26	leaving	leave	VBG
cord-104268-q1jx0n0l	116	27	intact	intact	JJ
cord-104268-q1jx0n0l	116	28	tightly	tightly	RB
cord-104268-q1jx0n0l	116	29	associated	associate	VBD
cord-104268-q1jx0n0l	116	30	membrane	membrane	NN
cord-104268-q1jx0n0l	116	31	proteins	protein	NNS
cord-104268-q1jx0n0l	116	32	.	.	.
cord-104268-q1jx0n0l	117	1	Ceils	Ceils	NNP
cord-104268-q1jx0n0l	117	2	expressing	express	VBG
cord-104268-q1jx0n0l	117	3	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	117	4	at	at	IN
cord-104268-q1jx0n0l	117	5	high	high	JJ
cord-104268-q1jx0n0l	117	6	levels	level	NNS
cord-104268-q1jx0n0l	117	7	were	be	VBD
cord-104268-q1jx0n0l	117	8	lysed	lyse	VBN
cord-104268-q1jx0n0l	117	9	with	with	IN
cord-104268-q1jx0n0l	117	10	glass	glass	NN
cord-104268-q1jx0n0l	117	11	beads	bead	NNS
cord-104268-q1jx0n0l	117	12	and	and	CC
cord-104268-q1jx0n0l	117	13	treated	treat	VBN
cord-104268-q1jx0n0l	117	14	with	with	IN
cord-104268-q1jx0n0l	117	15	sodium	sodium	NN
cord-104268-q1jx0n0l	117	16	carbonate	carbonate	NN
cord-104268-q1jx0n0l	117	17	or	or	CC
cord-104268-q1jx0n0l	117	18	urea	urea	NN
cord-104268-q1jx0n0l	117	19	.	.	.
cord-104268-q1jx0n0l	118	1	After	after	IN
cord-104268-q1jx0n0l	118	2	high	high	JJ
cord-104268-q1jx0n0l	118	3	-	-	HYPH
cord-104268-q1jx0n0l	118	4	speed	speed	NN
cord-104268-q1jx0n0l	118	5	centrifugation	centrifugation	NN
cord-104268-q1jx0n0l	118	6	of	of	IN
cord-104268-q1jx0n0l	118	7	the	the	DT
cord-104268-q1jx0n0l	118	8	treated	treat	VBN
cord-104268-q1jx0n0l	118	9	cell	cell	NN
cord-104268-q1jx0n0l	118	10	lysates	lysate	NNS
cord-104268-q1jx0n0l	118	11	,	,	,
cord-104268-q1jx0n0l	118	12	the	the	DT
cord-104268-q1jx0n0l	118	13	distribution	distribution	NN
cord-104268-q1jx0n0l	118	14	of	of	IN
cord-104268-q1jx0n0l	118	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	118	16	in	in	IN
cord-104268-q1jx0n0l	118	17	the	the	DT
cord-104268-q1jx0n0l	118	18	pellet	pellet	NN
cord-104268-q1jx0n0l	118	19	and	and	CC
cord-104268-q1jx0n0l	118	20	supernatant	supernatant	JJ
cord-104268-q1jx0n0l	118	21	fractions	fraction	NNS
cord-104268-q1jx0n0l	118	22	was	be	VBD
cord-104268-q1jx0n0l	118	23	assessed	assess	VBN
cord-104268-q1jx0n0l	118	24	by	by	IN
cord-104268-q1jx0n0l	118	25	Western	western	JJ
cord-104268-q1jx0n0l	118	26	immunoblotting	immunoblotting	NN
cord-104268-q1jx0n0l	118	27	.	.	.
cord-104268-q1jx0n0l	119	1	As	as	IN
cord-104268-q1jx0n0l	119	2	can	can	MD
cord-104268-q1jx0n0l	119	3	be	be	VB
cord-104268-q1jx0n0l	119	4	seen	see	VBN
cord-104268-q1jx0n0l	119	5	in	in	IN
cord-104268-q1jx0n0l	119	6	Fig	Fig	NNP
cord-104268-q1jx0n0l	119	7	.	.	.
cord-104268-q1jx0n0l	120	1	1	1	CD
cord-104268-q1jx0n0l	120	2	C	C	NNP
cord-104268-q1jx0n0l	120	3	,	,	,
cord-104268-q1jx0n0l	120	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	120	5	was	be	VBD
cord-104268-q1jx0n0l	120	6	found	find	VBN
cord-104268-q1jx0n0l	120	7	only	only	RB
cord-104268-q1jx0n0l	120	8	in	in	IN
cord-104268-q1jx0n0l	120	9	membrane	membrane	NN
cord-104268-q1jx0n0l	120	10	pellet	pellet	NN
cord-104268-q1jx0n0l	120	11	fractions	fraction	NNS
cord-104268-q1jx0n0l	120	12	.	.	.
cord-104268-q1jx0n0l	121	1	The	the	DT
cord-104268-q1jx0n0l	121	2	difference	difference	NN
cord-104268-q1jx0n0l	121	3	between	between	IN
cord-104268-q1jx0n0l	121	4	the	the	DT
cord-104268-q1jx0n0l	121	5	expected	expect	VBN
cord-104268-q1jx0n0l	121	6	(	(	-LRB-
cord-104268-q1jx0n0l	121	7	51.5	51.5	CD
cord-104268-q1jx0n0l	121	8	kD	kD	NNPS
cord-104268-q1jx0n0l	121	9	)	)	-RRB-
cord-104268-q1jx0n0l	121	10	and	and	CC
cord-104268-q1jx0n0l	121	11	observed	observe	VBD
cord-104268-q1jx0n0l	121	12	molecular	molecular	JJ
cord-104268-q1jx0n0l	121	13	mass	mass	NN
cord-104268-q1jx0n0l	121	14	of	of	IN
cord-104268-q1jx0n0l	121	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	122	1	(	(	-LRB-
cord-104268-q1jx0n0l	122	2	Fig	Fig	NNP
cord-104268-q1jx0n0l	122	3	.	.	.
cord-104268-q1jx0n0l	122	4	1	1	CD
cord-104268-q1jx0n0l	122	5	B	b	NN
cord-104268-q1jx0n0l	122	6	)	)	-RRB-
cord-104268-q1jx0n0l	122	7	is	be	VBZ
cord-104268-q1jx0n0l	122	8	likely	likely	JJ
cord-104268-q1jx0n0l	122	9	to	to	TO
cord-104268-q1jx0n0l	122	10	be	be	VB
cord-104268-q1jx0n0l	122	11	at	at	IN
cord-104268-q1jx0n0l	122	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	122	13	is	be	VBZ
cord-104268-q1jx0n0l	122	14	oriented	orient	VBN
cord-104268-q1jx0n0l	122	15	as	as	IN
cord-104268-q1jx0n0l	122	16	a	a	DT
cord-104268-q1jx0n0l	122	17	type	type	NN
cord-104268-q1jx0n0l	122	18	II	ii	CD
cord-104268-q1jx0n0l	122	19	membrane	membrane	NN
cord-104268-q1jx0n0l	122	20	-	-	HYPH
cord-104268-q1jx0n0l	122	21	anchored	anchor	VBN
cord-104268-q1jx0n0l	122	22	protein	protein	NN
cord-104268-q1jx0n0l	122	23	,	,	,
cord-104268-q1jx0n0l	122	24	a	a	DT
cord-104268-q1jx0n0l	122	25	topology	topology	NN
cord-104268-q1jx0n0l	122	26	characteristic	characteristic	JJ
cord-104268-q1jx0n0l	122	27	of	of	IN
cord-104268-q1jx0n0l	122	28	all	all	DT
cord-104268-q1jx0n0l	122	29	isolated	isolate	VBN
cord-104268-q1jx0n0l	122	30	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	122	31	(	(	-LRB-
cord-104268-q1jx0n0l	122	32	Shaper	shaper	NN
cord-104268-q1jx0n0l	122	33	and	and	CC
cord-104268-q1jx0n0l	122	34	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	122	35	,	,	,
cord-104268-q1jx0n0l	122	36	1992	1992	CD
cord-104268-q1jx0n0l	122	37	;	;	:
cord-104268-q1jx0n0l	122	38	Kleene	Kleene	NNP
cord-104268-q1jx0n0l	122	39	and	and	CC
cord-104268-q1jx0n0l	122	40	Berger	Berger	NNP
cord-104268-q1jx0n0l	122	41	,	,	,
cord-104268-q1jx0n0l	122	42	1993	1993	CD
cord-104268-q1jx0n0l	122	43	;	;	:
cord-104268-q1jx0n0l	122	44	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	122	45	et	et	NNP
cord-104268-q1jx0n0l	122	46	al	al	NNP
cord-104268-q1jx0n0l	122	47	.	.	NNP
cord-104268-q1jx0n0l	122	48	,	,	,
cord-104268-q1jx0n0l	122	49	1994	1994	CD
cord-104268-q1jx0n0l	122	50	)	)	-RRB-
cord-104268-q1jx0n0l	122	51	.	.	.
cord-104268-q1jx0n0l	123	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	123	2	consists	consist	VBZ
cord-104268-q1jx0n0l	123	3	of	of	IN
cord-104268-q1jx0n0l	123	4	a	a	DT
cord-104268-q1jx0n0l	123	5	short	short	JJ
cord-104268-q1jx0n0l	123	6	amino	amino	NN
cord-104268-q1jx0n0l	123	7	-	-	HYPH
cord-104268-q1jx0n0l	123	8	terminal	terminal	JJ
cord-104268-q1jx0n0l	123	9	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	123	10	domain	domain	NN
cord-104268-q1jx0n0l	123	11	,	,	,
cord-104268-q1jx0n0l	123	12	a	a	DT
cord-104268-q1jx0n0l	123	13	hydrophobic	hydrophobic	JJ
cord-104268-q1jx0n0l	123	14	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	123	15	domain	domain	NN
cord-104268-q1jx0n0l	123	16	,	,	,
cord-104268-q1jx0n0l	123	17	and	and	CC
cord-104268-q1jx0n0l	123	18	a	a	DT
cord-104268-q1jx0n0l	123	19	large	large	JJ
cord-104268-q1jx0n0l	123	20	carboxy	carboxy	JJ
cord-104268-q1jx0n0l	123	21	-	-	HYPH
cord-104268-q1jx0n0l	123	22	terminal	terminal	NN
cord-104268-q1jx0n0l	123	23	luminal	luminal	JJ
cord-104268-q1jx0n0l	123	24	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	123	25	domain	domain	NN
cord-104268-q1jx0n0l	123	26	.	.	.
cord-104268-q1jx0n0l	124	1	The	the	DT
cord-104268-q1jx0n0l	124	2	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	124	3	domain	domain	NN
cord-104268-q1jx0n0l	124	4	is	be	VBZ
cord-104268-q1jx0n0l	124	5	linked	link	VBN
cord-104268-q1jx0n0l	124	6	to	to	IN
cord-104268-q1jx0n0l	124	7	the	the	DT
cord-104268-q1jx0n0l	124	8	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	124	9	domain	domain	NN
cord-104268-q1jx0n0l	124	10	by	by	IN
cord-104268-q1jx0n0l	124	11	a	a	DT
cord-104268-q1jx0n0l	124	12	polypeptidic	polypeptidic	NNP
cord-104268-q1jx0n0l	124	13	"	"	``
cord-104268-q1jx0n0l	124	14	stem	stem	NN
cord-104268-q1jx0n0l	124	15	"	"	''
cord-104268-q1jx0n0l	124	16	region	region	NN
cord-104268-q1jx0n0l	124	17	.	.	.
cord-104268-q1jx0n0l	125	1	The	the	DT
cord-104268-q1jx0n0l	125	2	stem	stem	NN
cord-104268-q1jx0n0l	125	3	region	region	NN
cord-104268-q1jx0n0l	125	4	is	be	VBZ
cord-104268-q1jx0n0l	125	5	generally	generally	RB
cord-104268-q1jx0n0l	125	6	thought	think	VBN
cord-104268-q1jx0n0l	125	7	to	to	TO
cord-104268-q1jx0n0l	125	8	be	be	VB
cord-104268-q1jx0n0l	125	9	devoid	devoid	JJ
cord-104268-q1jx0n0l	125	10	of	of	IN
cord-104268-q1jx0n0l	125	11	secondary	secondary	JJ
cord-104268-q1jx0n0l	125	12	structure	structure	NN
cord-104268-q1jx0n0l	125	13	.	.	.
cord-104268-q1jx0n0l	126	1	(	(	-LRB-
cord-104268-q1jx0n0l	126	2	B	b	NN
cord-104268-q1jx0n0l	126	3	)	)	-RRB-
cord-104268-q1jx0n0l	126	4	Immunological	immunological	JJ
cord-104268-q1jx0n0l	126	5	detection	detection	NN
cord-104268-q1jx0n0l	126	6	of	of	IN
cord-104268-q1jx0n0l	126	7	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	126	8	.	.	.
cord-104268-q1jx0n0l	127	1	Yeast	yeast	JJ
cord-104268-q1jx0n0l	127	2	total	total	JJ
cord-104268-q1jx0n0l	127	3	-	-	HYPH
cord-104268-q1jx0n0l	127	4	protein	protein	NN
cord-104268-q1jx0n0l	127	5	extracts	extract	NNS
cord-104268-q1jx0n0l	127	6	from	from	IN
cord-104268-q1jx0n0l	127	7	kre2::TRP1	kre2::TRP1	NNP
cord-104268-q1jx0n0l	127	8	in	in	IN
cord-104268-q1jx0n0l	127	9	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	127	10	and	and	CC
cord-104268-q1jx0n0l	127	11	from	from	IN
cord-104268-q1jx0n0l	127	12	the	the	DT
cord-104268-q1jx0n0l	127	13	same	same	JJ
cord-104268-q1jx0n0l	127	14	strain	strain	NN
cord-104268-q1jx0n0l	127	15	expressing	express	VBG
cord-104268-q1jx0n0l	127	16	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	127	17	from	from	IN
cord-104268-q1jx0n0l	127	18	the	the	DT
cord-104268-q1jx0n0l	127	19	multicopy	multicopy	NNP
cord-104268-q1jx0n0l	127	20	plasmid	plasmid	NNP
cord-104268-q1jx0n0l	127	21	YEp352	YEp352	NNP
cord-104268-q1jx0n0l	127	22	(	(	-LRB-
cord-104268-q1jx0n0l	127	23	Hill	Hill	NNP
cord-104268-q1jx0n0l	127	24	et	et	NNP
cord-104268-q1jx0n0l	127	25	al	al	NNP
cord-104268-q1jx0n0l	127	26	.	.	NNP
cord-104268-q1jx0n0l	127	27	,	,	,
cord-104268-q1jx0n0l	127	28	1986	1986	CD
cord-104268-q1jx0n0l	127	29	)	)	-RRB-
cord-104268-q1jx0n0l	127	30	,	,	,
cord-104268-q1jx0n0l	127	31	were	be	VBD
cord-104268-q1jx0n0l	127	32	immunoblotted	immunoblotte	VBN
cord-104268-q1jx0n0l	127	33	with	with	IN
cord-104268-q1jx0n0l	127	34	affinity	affinity	NN
cord-104268-q1jx0n0l	127	35	-	-	HYPH
cord-104268-q1jx0n0l	127	36	purified	purify	VBN
cord-104268-q1jx0n0l	127	37	anti	anti	JJ
cord-104268-q1jx0n0l	127	38	-	-	JJ
cord-104268-q1jx0n0l	127	39	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	127	40	polyclonal	polyclonal	JJ
cord-104268-q1jx0n0l	127	41	antibodies	antibody	NNS
cord-104268-q1jx0n0l	127	42	(	(	-LRB-
cord-104268-q1jx0n0l	127	43	see	see	VB
cord-104268-q1jx0n0l	127	44	Materials	material	NNS
cord-104268-q1jx0n0l	127	45	and	and	CC
cord-104268-q1jx0n0l	127	46	Methods	Methods	NNPS
cord-104268-q1jx0n0l	127	47	)	)	-RRB-
cord-104268-q1jx0n0l	127	48	.	.	.
cord-104268-q1jx0n0l	128	1	The	the	DT
cord-104268-q1jx0n0l	128	2	molecular	molecular	JJ
cord-104268-q1jx0n0l	128	3	mass	mass	NN
cord-104268-q1jx0n0l	128	4	standards	standard	NNS
cord-104268-q1jx0n0l	128	5	are	be	VBP
cord-104268-q1jx0n0l	128	6	shown	show	VBN
cord-104268-q1jx0n0l	128	7	in	in	IN
cord-104268-q1jx0n0l	128	8	kilodaltons	kilodalton	NNS
cord-104268-q1jx0n0l	128	9	.	.	.
cord-104268-q1jx0n0l	129	1	(	(	-LRB-
cord-104268-q1jx0n0l	129	2	C	C	NNP
cord-104268-q1jx0n0l	129	3	)	)	-RRB-
cord-104268-q1jx0n0l	129	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	129	5	is	be	VBZ
cord-104268-q1jx0n0l	129	6	membrane	membrane	NN
cord-104268-q1jx0n0l	129	7	associated	associate	VBN
cord-104268-q1jx0n0l	129	8	.	.	.
cord-104268-q1jx0n0l	130	1	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	130	2	cells	cell	NNS
cord-104268-q1jx0n0l	130	3	overexpressing	overexpresse	VBG
cord-104268-q1jx0n0l	130	4	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	130	5	were	be	VBD
cord-104268-q1jx0n0l	130	6	lysed	lyse	VBN
cord-104268-q1jx0n0l	130	7	,	,	,
cord-104268-q1jx0n0l	130	8	and	and	CC
cord-104268-q1jx0n0l	130	9	cell	cell	NN
cord-104268-q1jx0n0l	130	10	debris	debris	NN
cord-104268-q1jx0n0l	130	11	was	be	VBD
cord-104268-q1jx0n0l	130	12	removed	remove	VBN
cord-104268-q1jx0n0l	130	13	by	by	IN
cord-104268-q1jx0n0l	130	14	centrifugation	centrifugation	NN
cord-104268-q1jx0n0l	130	15	.	.	.
cord-104268-q1jx0n0l	131	1	The	the	DT
cord-104268-q1jx0n0l	131	2	resulting	result	VBG
cord-104268-q1jx0n0l	131	3	crude	crude	NN
cord-104268-q1jx0n0l	131	4	homogenate	homogenate	NNP
cord-104268-q1jx0n0l	131	5	was	be	VBD
cord-104268-q1jx0n0l	131	6	aliquoted	aliquote	VBN
cord-104268-q1jx0n0l	131	7	,	,	,
cord-104268-q1jx0n0l	131	8	and	and	CC
cord-104268-q1jx0n0l	131	9	fractions	fraction	NNS
cord-104268-q1jx0n0l	131	10	were	be	VBD
cord-104268-q1jx0n0l	131	11	rendered	render	VBN
cord-104268-q1jx0n0l	131	12	0.1	0.1	CD
cord-104268-q1jx0n0l	131	13	M	m	NN
cord-104268-q1jx0n0l	131	14	with	with	IN
cord-104268-q1jx0n0l	131	15	Na2CO	Na2CO	NNP
cord-104268-q1jx0n0l	131	16	3	3	CD
cord-104268-q1jx0n0l	131	17	or	or	CC
cord-104268-q1jx0n0l	131	18	1.6	1.6	CD
cord-104268-q1jx0n0l	131	19	M	M	NNP
cord-104268-q1jx0n0l	131	20	with	with	IN
cord-104268-q1jx0n0l	131	21	urea	urea	NN
cord-104268-q1jx0n0l	131	22	.	.	.
cord-104268-q1jx0n0l	132	1	After	after	IN
cord-104268-q1jx0n0l	132	2	30	30	CD
cord-104268-q1jx0n0l	132	3	min	min	NN
cord-104268-q1jx0n0l	132	4	,	,	,
cord-104268-q1jx0n0l	132	5	each	each	DT
cord-104268-q1jx0n0l	132	6	fraction	fraction	NN
cord-104268-q1jx0n0l	132	7	was	be	VBD
cord-104268-q1jx0n0l	132	8	centrifuged	centrifuge	VBN
cord-104268-q1jx0n0l	132	9	at	at	IN
cord-104268-q1jx0n0l	132	10	100,000	100,000	CD
cord-104268-q1jx0n0l	132	11	g	g	NN
cord-104268-q1jx0n0l	132	12	for	for	IN
cord-104268-q1jx0n0l	132	13	1	1	CD
cord-104268-q1jx0n0l	132	14	h	h	NN
cord-104268-q1jx0n0l	132	15	and	and	CC
cord-104268-q1jx0n0l	132	16	after	after	IN
cord-104268-q1jx0n0l	132	17	SDS	SDS	NNP
cord-104268-q1jx0n0l	132	18	-	-	HYPH
cord-104268-q1jx0n0l	132	19	PAGE	PAGE	NNP
cord-104268-q1jx0n0l	132	20	,	,	,
cord-104268-q1jx0n0l	132	21	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	132	22	was	be	VBD
cord-104268-q1jx0n0l	132	23	detected	detect	VBN
cord-104268-q1jx0n0l	132	24	by	by	IN
cord-104268-q1jx0n0l	132	25	immunoblotting	immunoblotte	VBG
cord-104268-q1jx0n0l	132	26	with	with	IN
cord-104268-q1jx0n0l	132	27	the	the	DT
cord-104268-q1jx0n0l	132	28	anti	anti	JJ
cord-104268-q1jx0n0l	132	29	-	-	JJ
cord-104268-q1jx0n0l	132	30	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	132	31	Ab	Ab	NNP
cord-104268-q1jx0n0l	132	32	in	in	IN
cord-104268-q1jx0n0l	132	33	pellet	pellet	NN
cord-104268-q1jx0n0l	132	34	(	(	-LRB-
cord-104268-q1jx0n0l	132	35	P	p	NN
cord-104268-q1jx0n0l	132	36	)	)	-RRB-
cord-104268-q1jx0n0l	132	37	and	and	CC
cord-104268-q1jx0n0l	132	38	supernatant	supernatant	JJ
cord-104268-q1jx0n0l	132	39	(	(	-LRB-
cord-104268-q1jx0n0l	132	40	S	s	NN
cord-104268-q1jx0n0l	132	41	)	)	-RRB-
cord-104268-q1jx0n0l	132	42	fractions	fraction	NNS
cord-104268-q1jx0n0l	132	43	.	.	.
cord-104268-q1jx0n0l	133	1	least	least	JJS
cord-104268-q1jx0n0l	133	2	partly	partly	RB
cord-104268-q1jx0n0l	133	3	due	due	JJ
cord-104268-q1jx0n0l	133	4	to	to	IN
cord-104268-q1jx0n0l	133	5	N	n	CD
cord-104268-q1jx0n0l	133	6	-	-	HYPH
cord-104268-q1jx0n0l	133	7	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	133	8	,	,	,
cord-104268-q1jx0n0l	133	9	since	since	IN
cord-104268-q1jx0n0l	133	10	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	133	11	is	be	VBZ
cord-104268-q1jx0n0l	133	12	expected	expect	VBN
cord-104268-q1jx0n0l	133	13	to	to	TO
cord-104268-q1jx0n0l	133	14	act	act	VB
cord-104268-q1jx0n0l	133	15	in	in	IN
cord-104268-q1jx0n0l	133	16	the	the	DT
cord-104268-q1jx0n0l	133	17	secretory	secretory	JJ
cord-104268-q1jx0n0l	133	18	pathway	pathway	NN
cord-104268-q1jx0n0l	133	19	and	and	CC
cord-104268-q1jx0n0l	133	20	the	the	DT
cord-104268-q1jx0n0l	133	21	protein	protein	NN
cord-104268-q1jx0n0l	133	22	possesses	possess	VBZ
cord-104268-q1jx0n0l	133	23	a	a	DT
cord-104268-q1jx0n0l	133	24	single	single	JJ
cord-104268-q1jx0n0l	133	25	site	site	NN
cord-104268-q1jx0n0l	133	26	for	for	IN
cord-104268-q1jx0n0l	133	27	N	n	CD
cord-104268-q1jx0n0l	133	28	-	-	HYPH
cord-104268-q1jx0n0l	133	29	glycosyl	glycosyl	JJ
cord-104268-q1jx0n0l	133	30	attachment	attachment	NN
cord-104268-q1jx0n0l	133	31	in	in	IN
cord-104268-q1jx0n0l	133	32	its	-PRON-	PRP$
cord-104268-q1jx0n0l	133	33	predicted	predict	VBN
cord-104268-q1jx0n0l	133	34	luminal	luminal	JJ
cord-104268-q1jx0n0l	133	35	domain	domain	NN
cord-104268-q1jx0n0l	133	36	(	(	-LRB-
cord-104268-q1jx0n0l	133	37	Asna97-Gln	Asna97-Gln	NNP
cord-104268-q1jx0n0l	133	38	-	-	HYPH
cord-104268-q1jx0n0l	133	39	Thr	Thr	NNP
cord-104268-q1jx0n0l	133	40	)	)	-RRB-
cord-104268-q1jx0n0l	133	41	(	(	-LRB-
cord-104268-q1jx0n0l	133	42	see	see	VB
cord-104268-q1jx0n0l	133	43	Fig	Fig	NNP
cord-104268-q1jx0n0l	133	44	.	.	.
cord-104268-q1jx0n0l	133	45	6	6	CD
cord-104268-q1jx0n0l	133	46	)	)	-RRB-
cord-104268-q1jx0n0l	133	47	.	.	.
cord-104268-q1jx0n0l	134	1	To	to	TO
cord-104268-q1jx0n0l	134	2	test	test	VB
cord-104268-q1jx0n0l	134	3	for	for	IN
cord-104268-q1jx0n0l	134	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	134	5	N	n	CD
cord-104268-q1jx0n0l	134	6	-	-	HYPH
cord-104268-q1jx0n0l	134	7	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	134	8	,	,	,
cord-104268-q1jx0n0l	134	9	yeast	yeast	NN
cord-104268-q1jx0n0l	134	10	cells	cell	NNS
cord-104268-q1jx0n0l	134	11	were	be	VBD
cord-104268-q1jx0n0l	134	12	[	[	-LRB-
cord-104268-q1jx0n0l	134	13	35S]methionine	35s]methionine	CD
cord-104268-q1jx0n0l	134	14	labeled	label	VBN
cord-104268-q1jx0n0l	134	15	in	in	IN
cord-104268-q1jx0n0l	134	16	the	the	DT
cord-104268-q1jx0n0l	134	17	presence	presence	NN
cord-104268-q1jx0n0l	134	18	or	or	CC
cord-104268-q1jx0n0l	134	19	absence	absence	NN
cord-104268-q1jx0n0l	134	20	of	of	IN
cord-104268-q1jx0n0l	134	21	tunicamycin	tunicamycin	NNS
cord-104268-q1jx0n0l	134	22	,	,	,
cord-104268-q1jx0n0l	134	23	an	an	DT
cord-104268-q1jx0n0l	134	24	inhibitor	inhibitor	NN
cord-104268-q1jx0n0l	134	25	of	of	IN
cord-104268-q1jx0n0l	134	26	N	n	NN
cord-104268-q1jx0n0l	134	27	-	-	HYPH
cord-104268-q1jx0n0l	134	28	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	134	29	.	.	.
cord-104268-q1jx0n0l	135	1	Immunoprecipitation	Immunoprecipitation	NNP
cord-104268-q1jx0n0l	135	2	and	and	CC
cord-104268-q1jx0n0l	135	3	S	S	NNP
cord-104268-q1jx0n0l	135	4	D	D	NNP
cord-104268-q1jx0n0l	135	5	S	S	NNP
cord-104268-q1jx0n0l	135	6	-P	-p	NN
cord-104268-q1jx0n0l	136	1	A	a	DT
cord-104268-q1jx0n0l	136	2	G	g	NN
cord-104268-q1jx0n0l	136	3	E	e	NN
cord-104268-q1jx0n0l	136	4	analysis	analysis	NN
cord-104268-q1jx0n0l	136	5	of	of	IN
cord-104268-q1jx0n0l	136	6	labeled	label	VBN
cord-104268-q1jx0n0l	136	7	cell	cell	NN
cord-104268-q1jx0n0l	136	8	lysates	lysate	NNS
cord-104268-q1jx0n0l	136	9	revealed	reveal	VBD
cord-104268-q1jx0n0l	136	10	that	that	IN
cord-104268-q1jx0n0l	136	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	136	12	was	be	VBD
cord-104268-q1jx0n0l	136	13	N	n	CD
cord-104268-q1jx0n0l	136	14	-	-	HYPH
cord-104268-q1jx0n0l	136	15	glycosylated	glycosylated	JJ
cord-104268-q1jx0n0l	136	16	(	(	-LRB-
cord-104268-q1jx0n0l	136	17	see	see	VB
cord-104268-q1jx0n0l	136	18	Fig	Fig	NNP
cord-104268-q1jx0n0l	136	19	.	.	.
cord-104268-q1jx0n0l	137	1	3	3	LS
cord-104268-q1jx0n0l	137	2	,	,	,
cord-104268-q1jx0n0l	137	3	lanes	lane	NNS
cord-104268-q1jx0n0l	137	4	1	1	CD
cord-104268-q1jx0n0l	137	5	and	and	CC
cord-104268-q1jx0n0l	137	6	2	2	CD
cord-104268-q1jx0n0l	137	7	)	)	-RRB-
cord-104268-q1jx0n0l	137	8	,	,	,
cord-104268-q1jx0n0l	137	9	with	with	IN
cord-104268-q1jx0n0l	137	10	the	the	DT
cord-104268-q1jx0n0l	137	11	position	position	NN
cord-104268-q1jx0n0l	137	12	of	of	IN
cord-104268-q1jx0n0l	137	13	the	the	DT
cord-104268-q1jx0n0l	137	14	sole	sole	JJ
cord-104268-q1jx0n0l	137	15	N	n	CD
cord-104268-q1jx0n0l	137	16	-	-	HYPH
cord-104268-q1jx0n0l	137	17	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	137	18	attachment	attachment	NN
cord-104268-q1jx0n0l	137	19	site	site	NN
cord-104268-q1jx0n0l	137	20	at	at	IN
cord-104268-q1jx0n0l	137	21	A	A	NNP
cord-104268-q1jx0n0l	137	22	s	s	NN
cord-104268-q1jx0n0l	137	23	n	n	NN
cord-104268-q1jx0n0l	137	24	197	197	CD
cord-104268-q1jx0n0l	137	25	being	be	VBG
cord-104268-q1jx0n0l	137	26	consistent	consistent	JJ
cord-104268-q1jx0n0l	137	27	with	with	IN
cord-104268-q1jx0n0l	137	28	a	a	DT
cord-104268-q1jx0n0l	137	29	type	type	NN
cord-104268-q1jx0n0l	137	30	II	ii	CD
cord-104268-q1jx0n0l	137	31	topology	topology	NN
cord-104268-q1jx0n0l	137	32	for	for	IN
cord-104268-q1jx0n0l	137	33	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	137	34	.	.	.
cord-104268-q1jx0n0l	138	1	The	the	DT
cord-104268-q1jx0n0l	138	2	molecular	molecular	JJ
cord-104268-q1jx0n0l	138	3	mass	mass	NN
cord-104268-q1jx0n0l	138	4	of	of	IN
cord-104268-q1jx0n0l	138	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	138	6	in	in	IN
cord-104268-q1jx0n0l	138	7	the	the	DT
cord-104268-q1jx0n0l	138	8	presence	presence	NN
cord-104268-q1jx0n0l	138	9	of	of	IN
cord-104268-q1jx0n0l	138	10	tunicamycin	tunicamycin	NNP
cord-104268-q1jx0n0l	138	11	is	be	VBZ
cord-104268-q1jx0n0l	138	12	about	about	RB
cord-104268-q1jx0n0l	138	13	54	54	CD
cord-104268-q1jx0n0l	138	14	kD	kd	XX
cord-104268-q1jx0n0l	138	15	,	,	,
cord-104268-q1jx0n0l	138	16	still	still	RB
cord-104268-q1jx0n0l	138	17	2.5	2.5	CD
cord-104268-q1jx0n0l	138	18	kD	kd	JJ
cord-104268-q1jx0n0l	138	19	larger	large	JJR
cord-104268-q1jx0n0l	138	20	than	than	IN
cord-104268-q1jx0n0l	138	21	its	-PRON-	PRP$
cord-104268-q1jx0n0l	138	22	pre	pre	JJ
cord-104268-q1jx0n0l	138	23	-	-	JJ
cord-104268-q1jx0n0l	138	24	dicted	dicted	JJ
cord-104268-q1jx0n0l	138	25	molecular	molecular	JJ
cord-104268-q1jx0n0l	138	26	mass	mass	NN
cord-104268-q1jx0n0l	138	27	.	.	.
cord-104268-q1jx0n0l	139	1	The	the	DT
cord-104268-q1jx0n0l	139	2	membrane	membrane	NN
cord-104268-q1jx0n0l	139	3	nature	nature	NN
cord-104268-q1jx0n0l	139	4	of	of	IN
cord-104268-q1jx0n0l	139	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	139	6	or	or	CC
cord-104268-q1jx0n0l	139	7	other	other	JJ
cord-104268-q1jx0n0l	139	8	posttranslational	posttranslational	JJ
cord-104268-q1jx0n0l	139	9	modifications	modification	NNS
cord-104268-q1jx0n0l	139	10	could	could	MD
cord-104268-q1jx0n0l	139	11	explain	explain	VB
cord-104268-q1jx0n0l	139	12	this	this	DT
cord-104268-q1jx0n0l	139	13	discrepancy	discrepancy	NN
cord-104268-q1jx0n0l	139	14	.	.	.
cord-104268-q1jx0n0l	140	1	To	to	TO
cord-104268-q1jx0n0l	140	2	establish	establish	VB
cord-104268-q1jx0n0l	140	3	possible	possible	JJ
cord-104268-q1jx0n0l	140	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	140	5	modifications	modification	NNS
cord-104268-q1jx0n0l	140	6	that	that	WDT
cord-104268-q1jx0n0l	140	7	Kre2p	Kre2p	MD
cord-104268-q1jx0n0l	140	8	might	may	MD
cord-104268-q1jx0n0l	140	9	acquire	acquire	VB
cord-104268-q1jx0n0l	140	10	,	,	,
cord-104268-q1jx0n0l	140	11	and	and	CC
cord-104268-q1jx0n0l	140	12	to	to	TO
cord-104268-q1jx0n0l	140	13	attempt	attempt	VB
cord-104268-q1jx0n0l	140	14	to	to	TO
cord-104268-q1jx0n0l	140	15	assess	assess	VB
cord-104268-q1jx0n0l	140	16	to	to	IN
cord-104268-q1jx0n0l	140	17	which	which	WDT
cord-104268-q1jx0n0l	140	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	140	19	subcompartments	subcompartment	NNS
cord-104268-q1jx0n0l	140	20	it	-PRON-	PRP
cord-104268-q1jx0n0l	140	21	had	have	VBD
cord-104268-q1jx0n0l	140	22	been	be	VBN
cord-104268-q1jx0n0l	140	23	exposed	expose	VBN
cord-104268-q1jx0n0l	140	24	,	,	,
cord-104268-q1jx0n0l	140	25	the	the	DT
cord-104268-q1jx0n0l	140	26	size	size	NN
cord-104268-q1jx0n0l	140	27	of	of	IN
cord-104268-q1jx0n0l	140	28	the	the	DT
cord-104268-q1jx0n0l	140	29	Kre2plinked	kre2plinked	JJ
cord-104268-q1jx0n0l	140	30	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	140	31	chains	chain	NNS
cord-104268-q1jx0n0l	140	32	was	be	VBD
cord-104268-q1jx0n0l	140	33	examined	examine	VBN
cord-104268-q1jx0n0l	140	34	in	in	IN
cord-104268-q1jx0n0l	140	35	specific	specific	JJ
cord-104268-q1jx0n0l	140	36	N	n	CD
cord-104268-q1jx0n0l	140	37	-	-	HYPH
cord-104268-q1jx0n0l	140	38	linked	link	VBN
cord-104268-q1jx0n0l	140	39	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	140	40	mutants	mutant	NNS
cord-104268-q1jx0n0l	140	41	.	.	.
cord-104268-q1jx0n0l	141	1	The	the	DT
cord-104268-q1jx0n0l	141	2	mnn	mnn	NNP
cord-104268-q1jx0n0l	141	3	strains	strain	NNS
cord-104268-q1jx0n0l	141	4	carry	carry	VBP
cord-104268-q1jx0n0l	141	5	mutations	mutation	NNS
cord-104268-q1jx0n0l	141	6	at	at	IN
cord-104268-q1jx0n0l	141	7	various	various	JJ
cord-104268-q1jx0n0l	141	8	loci	locus	NNS
cord-104268-q1jx0n0l	141	9	resulting	result	VBG
cord-104268-q1jx0n0l	141	10	in	in	IN
cord-104268-q1jx0n0l	141	11	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	141	12	defects	defect	NNS
cord-104268-q1jx0n0l	141	13	(	(	-LRB-
cord-104268-q1jx0n0l	141	14	Fig	Fig	NNP
cord-104268-q1jx0n0l	141	15	.	.	NNP
cord-104268-q1jx0n0l	141	16	2	2	CD
cord-104268-q1jx0n0l	141	17	;	;	:
cord-104268-q1jx0n0l	141	18	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	141	19	,	,	,
cord-104268-q1jx0n0l	141	20	1990	1990	CD
cord-104268-q1jx0n0l	141	21	;	;	:
cord-104268-q1jx0n0l	141	22	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	141	23	et	et	NNP
cord-104268-q1jx0n0l	141	24	al	al	NNP
cord-104268-q1jx0n0l	141	25	.	.	NNP
cord-104268-q1jx0n0l	141	26	,	,	,
cord-104268-q1jx0n0l	141	27	1990	1990	CD
cord-104268-q1jx0n0l	141	28	)	)	-RRB-
cord-104268-q1jx0n0l	141	29	.	.	.
cord-104268-q1jx0n0l	142	1	The	the	DT
cord-104268-q1jx0n0l	142	2	N	N	NNP
cord-104268-q1jx0n0l	142	3	-	-	HYPH
cord-104268-q1jx0n0l	142	4	linked	link	VBN
cord-104268-q1jx0n0l	142	5	carbohydrates	carbohydrate	NNS
cord-104268-q1jx0n0l	142	6	from	from	IN
cord-104268-q1jx0n0l	142	7	an	an	DT
cord-104268-q1jx0n0l	142	8	mnn9	mnn9	CD
cord-104268-q1jx0n0l	142	9	strain	strain	NN
cord-104268-q1jx0n0l	142	10	lack	lack	VBP
cord-104268-q1jx0n0l	142	11	the	the	DT
cord-104268-q1jx0n0l	142	12	outer	outer	JJ
cord-104268-q1jx0n0l	142	13	chain	chain	NN
cord-104268-q1jx0n0l	142	14	.	.	.
cord-104268-q1jx0n0l	143	1	The	the	DT
cord-104268-q1jx0n0l	143	2	mnnl	mnnl	NNS
cord-104268-q1jx0n0l	143	3	strain	strain	NN
cord-104268-q1jx0n0l	143	4	produces	produce	VBZ
cord-104268-q1jx0n0l	143	5	glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	143	6	with	with	IN
cord-104268-q1jx0n0l	143	7	N	n	CD
cord-104268-q1jx0n0l	143	8	-	-	HYPH
cord-104268-q1jx0n0l	143	9	linked	link	VBN
cord-104268-q1jx0n0l	143	10	chains	chain	NNS
cord-104268-q1jx0n0l	143	11	lacking	lack	VBG
cord-104268-q1jx0n0l	143	12	terminal	terminal	NN
cord-104268-q1jx0n0l	143	13	c	c	NN
cord-104268-q1jx0n0l	143	14	~	~	NFP
cord-104268-q1jx0n0l	143	15	l,3-mannosyl	l,3-mannosyl	NNP
cord-104268-q1jx0n0l	143	16	residues	residue	NNS
cord-104268-q1jx0n0l	143	17	(	(	-LRB-
cord-104268-q1jx0n0l	143	18	Fig	Fig	NNP
cord-104268-q1jx0n0l	143	19	.	.	NNP
cord-104268-q1jx0n0l	143	20	2	2	CD
cord-104268-q1jx0n0l	143	21	)	)	-RRB-
cord-104268-q1jx0n0l	143	22	.	.	.
cord-104268-q1jx0n0l	144	1	Wild	wild	JJ
cord-104268-q1jx0n0l	144	2	-	-	HYPH
cord-104268-q1jx0n0l	144	3	type	type	NN
cord-104268-q1jx0n0l	144	4	,	,	,
cord-104268-q1jx0n0l	144	5	mnnl	mnnl	NNS
cord-104268-q1jx0n0l	144	6	,	,	,
cord-104268-q1jx0n0l	144	7	and	and	CC
cord-104268-q1jx0n0l	144	8	mnn9	mnn9	CD
cord-104268-q1jx0n0l	144	9	mutant	mutant	JJ
cord-104268-q1jx0n0l	144	10	yeast	yeast	NN
cord-104268-q1jx0n0l	144	11	strains	strain	NNS
cord-104268-q1jx0n0l	144	12	carrying	carry	VBG
cord-104268-q1jx0n0l	144	13	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	144	14	were	be	VBD
cord-104268-q1jx0n0l	144	15	[	[	-LRB-
cord-104268-q1jx0n0l	144	16	35S]methionine	35s]methionine	CD
cord-104268-q1jx0n0l	144	17	labeled	label	VBN
cord-104268-q1jx0n0l	144	18	for	for	IN
cord-104268-q1jx0n0l	144	19	10	10	CD
cord-104268-q1jx0n0l	144	20	rain	rain	NN
cord-104268-q1jx0n0l	144	21	and	and	CC
cord-104268-q1jx0n0l	144	22	chased	chase	VBD
cord-104268-q1jx0n0l	144	23	for	for	IN
cord-104268-q1jx0n0l	144	24	45	45	CD
cord-104268-q1jx0n0l	144	25	min	min	NN
cord-104268-q1jx0n0l	144	26	,	,	,
cord-104268-q1jx0n0l	144	27	and	and	CC
cord-104268-q1jx0n0l	144	28	the	the	DT
cord-104268-q1jx0n0l	144	29	extent	extent	NN
cord-104268-q1jx0n0l	144	30	of	of	IN
cord-104268-q1jx0n0l	144	31	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	144	32	of	of	IN
cord-104268-q1jx0n0l	144	33	immunoprecipitated	immunoprecipitated	JJ
cord-104268-q1jx0n0l	144	34	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	144	35	was	be	VBD
cord-104268-q1jx0n0l	144	36	examined	examine	VBN
cord-104268-q1jx0n0l	144	37	(	(	-LRB-
cord-104268-q1jx0n0l	144	38	Fig	Fig	NNP
cord-104268-q1jx0n0l	144	39	.	.	.
cord-104268-q1jx0n0l	144	40	3	3	CD
cord-104268-q1jx0n0l	144	41	)	)	-RRB-
cord-104268-q1jx0n0l	144	42	.	.	.
cord-104268-q1jx0n0l	145	1	The	the	DT
cord-104268-q1jx0n0l	145	2	size	size	NN
cord-104268-q1jx0n0l	145	3	of	of	IN
cord-104268-q1jx0n0l	145	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	145	5	produced	produce	VBN
cord-104268-q1jx0n0l	145	6	in	in	IN
cord-104268-q1jx0n0l	145	7	a	a	DT
cord-104268-q1jx0n0l	145	8	wild	wild	JJ
cord-104268-q1jx0n0l	145	9	-	-	HYPH
cord-104268-q1jx0n0l	145	10	type	type	NN
cord-104268-q1jx0n0l	145	11	strain	strain	NN
cord-104268-q1jx0n0l	145	12	after	after	IN
cord-104268-q1jx0n0l	145	13	a	a	DT
cord-104268-q1jx0n0l	145	14	10-min	10-min	CD
cord-104268-q1jx0n0l	145	15	radiolabeling	radiolabeling	NN
cord-104268-q1jx0n0l	145	16	is	be	VBZ
cord-104268-q1jx0n0l	145	17	~57	~57	NFP
cord-104268-q1jx0n0l	145	18	kD	kD	NNP
cord-104268-q1jx0n0l	145	19	(	(	-LRB-
cord-104268-q1jx0n0l	145	20	major	major	JJ
cord-104268-q1jx0n0l	145	21	band	band	NN
cord-104268-q1jx0n0l	145	22	,	,	,
cord-104268-q1jx0n0l	145	23	lane	lane	NN
cord-104268-q1jx0n0l	145	24	2	2	CD
cord-104268-q1jx0n0l	145	25	)	)	-RRB-
cord-104268-q1jx0n0l	145	26	.	.	.
cord-104268-q1jx0n0l	146	1	After	after	IN
cord-104268-q1jx0n0l	146	2	an	an	DT
cord-104268-q1jx0n0l	146	3	additional	additional	JJ
cord-104268-q1jx0n0l	146	4	45-min	45-min	CD
cord-104268-q1jx0n0l	146	5	chase	chase	NN
cord-104268-q1jx0n0l	146	6	,	,	,
cord-104268-q1jx0n0l	146	7	an	an	DT
cord-104268-q1jx0n0l	146	8	apparent	apparent	JJ
cord-104268-q1jx0n0l	146	9	increase	increase	NN
cord-104268-q1jx0n0l	146	10	of	of	IN
cord-104268-q1jx0n0l	146	11	molecular	molecular	JJ
cord-104268-q1jx0n0l	146	12	mass	mass	NN
cord-104268-q1jx0n0l	146	13	from	from	IN
cord-104268-q1jx0n0l	146	14	57	57	CD
cord-104268-q1jx0n0l	146	15	to	to	IN
cord-104268-q1jx0n0l	146	16	~59	~59	CD
cord-104268-q1jx0n0l	146	17	-	-	HYPH
cord-104268-q1jx0n0l	146	18	60	60	CD
cord-104268-q1jx0n0l	146	19	kD	kD	NNP
cord-104268-q1jx0n0l	146	20	was	be	VBD
cord-104268-q1jx0n0l	146	21	seen	see	VBN
cord-104268-q1jx0n0l	146	22	(	(	-LRB-
cord-104268-q1jx0n0l	146	23	Fig	Fig	NNP
cord-104268-q1jx0n0l	146	24	.	.	.
cord-104268-q1jx0n0l	146	25	3	3	CD
cord-104268-q1jx0n0l	146	26	,	,	,
cord-104268-q1jx0n0l	146	27	lanes	lane	NNS
cord-104268-q1jx0n0l	146	28	2	2	CD
cord-104268-q1jx0n0l	146	29	and	and	CC
cord-104268-q1jx0n0l	146	30	5	5	CD
cord-104268-q1jx0n0l	146	31	)	)	-RRB-
cord-104268-q1jx0n0l	146	32	.	.	.
cord-104268-q1jx0n0l	147	1	This	this	DT
cord-104268-q1jx0n0l	147	2	apparent	apparent	JJ
cord-104268-q1jx0n0l	147	3	2	2	CD
cord-104268-q1jx0n0l	147	4	-	-	SYM
cord-104268-q1jx0n0l	147	5	3	3	CD
cord-104268-q1jx0n0l	147	6	kD	kd	JJ
cord-104268-q1jx0n0l	147	7	increase	increase	NN
cord-104268-q1jx0n0l	147	8	in	in	IN
cord-104268-q1jx0n0l	147	9	mass	mass	NN
cord-104268-q1jx0n0l	147	10	suggests	suggest	VBZ
cord-104268-q1jx0n0l	147	11	that	that	IN
cord-104268-q1jx0n0l	147	12	Kre2p	kre2p	PRP
cord-104268-q1jx0n0l	147	13	may	may	MD
cord-104268-q1jx0n0l	147	14	undergo	undergo	VB
cord-104268-q1jx0n0l	147	15	a	a	DT
cord-104268-q1jx0n0l	147	16	post	post	JJ
cord-104268-q1jx0n0l	147	17	-	-	JJ
cord-104268-q1jx0n0l	147	18	ER	er	JJ
cord-104268-q1jx0n0l	147	19	modification	modification	NN
cord-104268-q1jx0n0l	147	20	not	not	RB
cord-104268-q1jx0n0l	147	21	involving	involve	VBG
cord-104268-q1jx0n0l	147	22	extensive	extensive	JJ
cord-104268-q1jx0n0l	147	23	outer	outer	JJ
cord-104268-q1jx0n0l	147	24	chain	chain	NN
cord-104268-q1jx0n0l	147	25	elaboration	elaboration	NN
cord-104268-q1jx0n0l	147	26	.	.	.
cord-104268-q1jx0n0l	148	1	The	the	DT
cord-104268-q1jx0n0l	148	2	molecular	molecular	JJ
cord-104268-q1jx0n0l	148	3	mass	mass	NN
cord-104268-q1jx0n0l	148	4	of	of	IN
cord-104268-q1jx0n0l	148	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	148	6	produced	produce	VBN
cord-104268-q1jx0n0l	148	7	in	in	IN
cord-104268-q1jx0n0l	148	8	an	an	DT
cord-104268-q1jx0n0l	148	9	mnn9	mnn9	CD
cord-104268-q1jx0n0l	148	10	strain	strain	NN
cord-104268-q1jx0n0l	148	11	after	after	IN
cord-104268-q1jx0n0l	148	12	a	a	DT
cord-104268-q1jx0n0l	148	13	10-min	10-min	CD
cord-104268-q1jx0n0l	148	14	pulse	pulse	NN
cord-104268-q1jx0n0l	148	15	(	(	-LRB-
cord-104268-q1jx0n0l	148	16	Fig	Fig	NNP
cord-104268-q1jx0n0l	148	17	.	.	.
cord-104268-q1jx0n0l	148	18	3	3	CD
cord-104268-q1jx0n0l	148	19	,	,	,
cord-104268-q1jx0n0l	148	20	lanes	lane	NNS
cord-104268-q1jx0n0l	148	21	2	2	CD
cord-104268-q1jx0n0l	148	22	and	and	CC
cord-104268-q1jx0n0l	148	23	4	4	CD
cord-104268-q1jx0n0l	148	24	)	)	-RRB-
cord-104268-q1jx0n0l	148	25	and	and	CC
cord-104268-q1jx0n0l	148	26	a	a	DT
cord-104268-q1jx0n0l	148	27	45	45	CD
cord-104268-q1jx0n0l	148	28	min	min	NN
cord-104268-q1jx0n0l	148	29	chase	chase	NNP
cord-104268-q1jx0n0l	148	30	(	(	-LRB-
cord-104268-q1jx0n0l	148	31	lanes	lane	NNS
cord-104268-q1jx0n0l	148	32	5	5	CD
cord-104268-q1jx0n0l	148	33	and	and	CC
cord-104268-q1jx0n0l	148	34	7	7	CD
cord-104268-q1jx0n0l	148	35	)	)	-RRB-
cord-104268-q1jx0n0l	148	36	was	be	VBD
cord-104268-q1jx0n0l	148	37	identical	identical	JJ
cord-104268-q1jx0n0l	148	38	to	to	IN
cord-104268-q1jx0n0l	148	39	the	the	DT
cord-104268-q1jx0n0l	148	40	mass	mass	NN
cord-104268-q1jx0n0l	148	41	of	of	IN
cord-104268-q1jx0n0l	148	42	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	148	43	from	from	IN
cord-104268-q1jx0n0l	148	44	a	a	DT
cord-104268-q1jx0n0l	148	45	wild	wild	JJ
cord-104268-q1jx0n0l	148	46	-	-	HYPH
cord-104268-q1jx0n0l	148	47	type	type	NN
cord-104268-q1jx0n0l	148	48	cell	cell	NN
cord-104268-q1jx0n0l	148	49	,	,	,
cord-104268-q1jx0n0l	148	50	indicating	indicate	VBG
cord-104268-q1jx0n0l	148	51	that	that	IN
cord-104268-q1jx0n0l	148	52	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	148	53	does	do	VBZ
cord-104268-q1jx0n0l	148	54	not	not	RB
cord-104268-q1jx0n0l	148	55	receive	receive	VB
cord-104268-q1jx0n0l	148	56	an	an	DT
cord-104268-q1jx0n0l	148	57	outer	outer	JJ
cord-104268-q1jx0n0l	148	58	chain	chain	NN
cord-104268-q1jx0n0l	148	59	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	148	60	.	.	.
cord-104268-q1jx0n0l	149	1	We	-PRON-	PRP
cord-104268-q1jx0n0l	149	2	tested	test	VBD
cord-104268-q1jx0n0l	149	3	whether	whether	IN
cord-104268-q1jx0n0l	149	4	the	the	DT
cord-104268-q1jx0n0l	149	5	time	time	NN
cord-104268-q1jx0n0l	149	6	-	-	HYPH
cord-104268-q1jx0n0l	149	7	dependent	dependent	JJ
cord-104268-q1jx0n0l	149	8	additional	additional	JJ
cord-104268-q1jx0n0l	149	9	carbohydrate	carbohydrate	NN
cord-104268-q1jx0n0l	149	10	modification	modification	NN
cord-104268-q1jx0n0l	149	11	required	require	VBD
cord-104268-q1jx0n0l	149	12	the	the	DT
cord-104268-q1jx0n0l	149	13	MNN1encoded	MNN1encoded	NNP
cord-104268-q1jx0n0l	149	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	149	15	al,3-mannosyltransferase	al,3-mannosyltransferase	NNP
cord-104268-q1jx0n0l	149	16	,	,	,
cord-104268-q1jx0n0l	149	17	an	an	DT
cord-104268-q1jx0n0l	149	18	enzyme	enzyme	NN
cord-104268-q1jx0n0l	149	19	that	that	WDT
cord-104268-q1jx0n0l	149	20	acts	act	VBZ
cord-104268-q1jx0n0l	149	21	on	on	IN
cord-104268-q1jx0n0l	149	22	both	both	DT
cord-104268-q1jx0n0l	149	23	core	core	NN
cord-104268-q1jx0n0l	149	24	and	and	CC
cord-104268-q1jx0n0l	149	25	outer	outer	JJ
cord-104268-q1jx0n0l	149	26	chains	chain	NNS
cord-104268-q1jx0n0l	149	27	(	(	-LRB-
cord-104268-q1jx0n0l	149	28	Fig	Fig	NNP
cord-104268-q1jx0n0l	149	29	.	.	NNP
cord-104268-q1jx0n0l	149	30	2	2	CD
cord-104268-q1jx0n0l	149	31	;	;	:
cord-104268-q1jx0n0l	149	32	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	149	33	et	et	NNP
cord-104268-q1jx0n0l	149	34	al	al	NNP
cord-104268-q1jx0n0l	149	35	.	.	NNP
cord-104268-q1jx0n0l	149	36	,	,	,
cord-104268-q1jx0n0l	149	37	1990	1990	CD
cord-104268-q1jx0n0l	149	38	;	;	:
cord-104268-q1jx0n0l	149	39	Graham	Graham	NNP
cord-104268-q1jx0n0l	149	40	and	and	CC
cord-104268-q1jx0n0l	149	41	Emr	Emr	NNP
cord-104268-q1jx0n0l	149	42	,	,	,
cord-104268-q1jx0n0l	149	43	1991	1991	CD
cord-104268-q1jx0n0l	149	44	;	;	:
cord-104268-q1jx0n0l	149	45	Graham	Graham	NNP
cord-104268-q1jx0n0l	149	46	et	et	NNP
cord-104268-q1jx0n0l	149	47	al	al	NNP
cord-104268-q1jx0n0l	149	48	.	.	NNP
cord-104268-q1jx0n0l	149	49	,	,	,
cord-104268-q1jx0n0l	149	50	1994	1994	CD
cord-104268-q1jx0n0l	149	51	;	;	:
cord-104268-q1jx0n0l	149	52	Yip	Yip	NNP
cord-104268-q1jx0n0l	149	53	et	et	NNP
cord-104268-q1jx0n0l	149	54	al	al	NNP
cord-104268-q1jx0n0l	149	55	.	.	NNP
cord-104268-q1jx0n0l	149	56	,	,	,
cord-104268-q1jx0n0l	149	57	1994	1994	CD
cord-104268-q1jx0n0l	149	58	)	)	-RRB-
cord-104268-q1jx0n0l	149	59	.	.	.
cord-104268-q1jx0n0l	150	1	After	after	IN
cord-104268-q1jx0n0l	150	2	a	a	DT
cord-104268-q1jx0n0l	150	3	short	short	JJ
cord-104268-q1jx0n0l	150	4	pulse	pulse	NN
cord-104268-q1jx0n0l	150	5	,	,	,
cord-104268-q1jx0n0l	150	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	150	7	synthesized	synthesize	VBN
cord-104268-q1jx0n0l	150	8	in	in	IN
cord-104268-q1jx0n0l	150	9	a	a	DT
cord-104268-q1jx0n0l	150	10	mnnl	mnnl	NNS
cord-104268-q1jx0n0l	150	11	strain	strain	NN
cord-104268-q1jx0n0l	150	12	is	be	VBZ
cord-104268-q1jx0n0l	150	13	approximately	approximately	RB
cord-104268-q1jx0n0l	150	14	of	of	IN
cord-104268-q1jx0n0l	150	15	wild	wild	JJ
cord-104268-q1jx0n0l	150	16	-	-	HYPH
cord-104268-q1jx0n0l	150	17	type	type	NN
cord-104268-q1jx0n0l	150	18	size	size	NN
cord-104268-q1jx0n0l	150	19	(	(	-LRB-
cord-104268-q1jx0n0l	150	20	Fig	Fig	NNP
cord-104268-q1jx0n0l	150	21	.	.	.
cord-104268-q1jx0n0l	150	22	3	3	CD
cord-104268-q1jx0n0l	150	23	,	,	,
cord-104268-q1jx0n0l	150	24	lane	lane	NN
cord-104268-q1jx0n0l	150	25	 	 	_SP
cord-104268-q1jx0n0l	150	26	3	3	CD
cord-104268-q1jx0n0l	150	27	)	)	-RRB-
cord-104268-q1jx0n0l	150	28	.	.	.
cord-104268-q1jx0n0l	151	1	After	after	IN
cord-104268-q1jx0n0l	151	2	a	a	DT
cord-104268-q1jx0n0l	151	3	45-min	45-min	CD
cord-104268-q1jx0n0l	151	4	chase	chase	NN
cord-104268-q1jx0n0l	151	5	,	,	,
cord-104268-q1jx0n0l	151	6	however	however	RB
cord-104268-q1jx0n0l	151	7	,	,	,
cord-104268-q1jx0n0l	151	8	the	the	DT
cord-104268-q1jx0n0l	151	9	mnnl	mnnl	NNS
cord-104268-q1jx0n0l	151	10	-	-	HYPH
cord-104268-q1jx0n0l	151	11	produced	produce	VBN
cord-104268-q1jx0n0l	151	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	151	13	was	be	VBD
cord-104268-q1jx0n0l	151	14	~1-	~1-	NNP
cord-104268-q1jx0n0l	152	1	2	2	LS
cord-104268-q1jx0n0l	152	2	kD	kd	VBP
cord-104268-q1jx0n0l	152	3	smaller	small	JJR
cord-104268-q1jx0n0l	152	4	than	than	IN
cord-104268-q1jx0n0l	152	5	the	the	DT
cord-104268-q1jx0n0l	152	6	wild	wild	JJ
cord-104268-q1jx0n0l	152	7	-	-	HYPH
cord-104268-q1jx0n0l	152	8	type	type	NN
cord-104268-q1jx0n0l	152	9	protein	protein	NN
cord-104268-q1jx0n0l	152	10	(	(	-LRB-
cord-104268-q1jx0n0l	152	11	lanes	lane	NNS
cord-104268-q1jx0n0l	152	12	5	5	CD
cord-104268-q1jx0n0l	152	13	-	-	SYM
cord-104268-q1jx0n0l	152	14	7	7	CD
cord-104268-q1jx0n0l	152	15	)	)	-RRB-
cord-104268-q1jx0n0l	152	16	indicating	indicate	VBG
cord-104268-q1jx0n0l	152	17	that	that	IN
cord-104268-q1jx0n0l	152	18	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	152	19	is	be	VBZ
cord-104268-q1jx0n0l	152	20	normally	normally	RB
cord-104268-q1jx0n0l	152	21	exposed	expose	VBN
cord-104268-q1jx0n0l	152	22	to	to	IN
cord-104268-q1jx0n0l	152	23	a	a	DT
cord-104268-q1jx0n0l	152	24	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	152	25	compartment	compartment	NN
cord-104268-q1jx0n0l	152	26	where	where	WRB
cord-104268-q1jx0n0l	152	27	the	the	DT
cord-104268-q1jx0n0l	152	28	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	152	29	cxl,3-mannosyltransferase	cxl,3-mannosyltransferase	NN
cord-104268-q1jx0n0l	152	30	adds	add	VBZ
cord-104268-q1jx0n0l	152	31	at	at	RB
cord-104268-q1jx0n0l	152	32	least	least	RBS
cord-104268-q1jx0n0l	152	33	three	three	CD
cord-104268-q1jx0n0l	152	34	mannose	mannose	NN
cord-104268-q1jx0n0l	152	35	residues	residue	NNS
cord-104268-q1jx0n0l	152	36	to	to	IN
cord-104268-q1jx0n0l	152	37	the	the	DT
cord-104268-q1jx0n0l	152	38	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	152	39	N	n	CD
cord-104268-q1jx0n0l	152	40	-	-	HYPH
cord-104268-q1jx0n0l	152	41	glycosyl	glycosyl	NN
cord-104268-q1jx0n0l	152	42	core	core	NN
cord-104268-q1jx0n0l	152	43	.	.	.
cord-104268-q1jx0n0l	153	1	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	153	2	therefore	therefore	RB
cord-104268-q1jx0n0l	153	3	contributes	contribute	VBZ
cord-104268-q1jx0n0l	153	4	to	to	IN
cord-104268-q1jx0n0l	153	5	most	most	JJS
cord-104268-q1jx0n0l	153	6	of	of	IN
cord-104268-q1jx0n0l	153	7	the	the	DT
cord-104268-q1jx0n0l	153	8	observed	observed	JJ
cord-104268-q1jx0n0l	153	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	153	10	post	post	JJ
cord-104268-q1jx0n0l	153	11	-	-	JJ
cord-104268-q1jx0n0l	153	12	ER	er	JJ
cord-104268-q1jx0n0l	153	13	modifications	modification	NNS
cord-104268-q1jx0n0l	153	14	(	(	-LRB-
cord-104268-q1jx0n0l	153	15	Fig	fig	NN
cord-104268-q1jx0n0l	153	16	.	.	NNP
cord-104268-q1jx0n0l	153	17	2	2	CD
cord-104268-q1jx0n0l	153	18	)	)	-RRB-
cord-104268-q1jx0n0l	153	19	.	.	.
cord-104268-q1jx0n0l	154	1	GlcNAc2	GlcNAc2	NNP
cord-104268-q1jx0n0l	154	2	carbohydrate	carbohydrate	NN
cord-104268-q1jx0n0l	154	3	structure	structure	NN
cord-104268-q1jx0n0l	154	4	.	.	.
cord-104268-q1jx0n0l	155	1	In	in	IN
cord-104268-q1jx0n0l	155	2	other	other	JJ
cord-104268-q1jx0n0l	155	3	cases	case	NNS
cord-104268-q1jx0n0l	155	4	,	,	,
cord-104268-q1jx0n0l	155	5	core	core	NN
cord-104268-q1jx0n0l	155	6	structures	structure	NNS
cord-104268-q1jx0n0l	155	7	may	may	MD
cord-104268-q1jx0n0l	155	8	be	be	VB
cord-104268-q1jx0n0l	155	9	extended	extend	VBN
cord-104268-q1jx0n0l	155	10	by	by	IN
cord-104268-q1jx0n0l	155	11	an	an	DT
cord-104268-q1jx0n0l	155	12	outer	outer	JJ
cord-104268-q1jx0n0l	155	13	chain	chain	NN
cord-104268-q1jx0n0l	155	14	of	of	IN
cord-104268-q1jx0n0l	155	15	variable	variable	JJ
cord-104268-q1jx0n0l	155	16	size	size	NN
cord-104268-q1jx0n0l	155	17	(	(	-LRB-
cord-104268-q1jx0n0l	155	18	up	up	IN
cord-104268-q1jx0n0l	155	19	to	to	TO
cord-104268-q1jx0n0l	155	20	200	200	CD
cord-104268-q1jx0n0l	155	21	mannose	mannose	NN
cord-104268-q1jx0n0l	155	22	residues	residue	NNS
cord-104268-q1jx0n0l	155	23	)	)	-RRB-
cord-104268-q1jx0n0l	155	24	that	that	WDT
cord-104268-q1jx0n0l	155	25	is	be	VBZ
cord-104268-q1jx0n0l	155	26	composed	compose	VBN
cord-104268-q1jx0n0l	155	27	of	of	IN
cord-104268-q1jx0n0l	155	28	a	a	DT
cord-104268-q1jx0n0l	155	29	backbone	backbone	NN
cord-104268-q1jx0n0l	155	30	of	of	IN
cord-104268-q1jx0n0l	155	31	al,6-mannosyl	al,6-mannosyl	JJ
cord-104268-q1jx0n0l	155	32	residues	residue	NNS
cord-104268-q1jx0n0l	155	33	to	to	TO
cord-104268-q1jx0n0l	155	34	which	which	WDT
cord-104268-q1jx0n0l	155	35	are	be	VBP
cord-104268-q1jx0n0l	155	36	attached	attach	VBN
cord-104268-q1jx0n0l	155	37	branched	branch	VBN
cord-104268-q1jx0n0l	155	38	al,2-and	al,2-and	NNP
cord-104268-q1jx0n0l	155	39	cd,3-mannosyl	cd,3-mannosyl	NN
cord-104268-q1jx0n0l	155	40	side	side	NN
cord-104268-q1jx0n0l	155	41	chains	chain	NNS
cord-104268-q1jx0n0l	155	42	.	.	.
cord-104268-q1jx0n0l	156	1	Recent	recent	JJ
cord-104268-q1jx0n0l	156	2	evidence	evidence	NN
cord-104268-q1jx0n0l	156	3	suggests	suggest	VBZ
cord-104268-q1jx0n0l	156	4	that	that	IN
cord-104268-q1jx0n0l	156	5	the	the	DT
cord-104268-q1jx0n0l	156	6	initiating	initiate	VBG
cord-104268-q1jx0n0l	156	7	Ochlp	Ochlp	NNP
cord-104268-q1jx0n0l	156	8	al,6-mannosyltransferase	al,6-mannosyltransferase	CD
cord-104268-q1jx0n0l	157	1	(	(	-LRB-
cord-104268-q1jx0n0l	157	2	Nakayama	Nakayama	NNP
cord-104268-q1jx0n0l	157	3	et	et	NNP
cord-104268-q1jx0n0l	157	4	al	al	NNP
cord-104268-q1jx0n0l	157	5	.	.	NNP
cord-104268-q1jx0n0l	157	6	,	,	,
cord-104268-q1jx0n0l	157	7	1992	1992	CD
cord-104268-q1jx0n0l	157	8	)	)	-RRB-
cord-104268-q1jx0n0l	157	9	defines	define	VBZ
cord-104268-q1jx0n0l	157	10	a	a	DT
cord-104268-q1jx0n0l	157	11	very	very	RB
cord-104268-q1jx0n0l	157	12	early	early	JJ
cord-104268-q1jx0n0l	157	13	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	157	14	compartment	compartment	NN
cord-104268-q1jx0n0l	157	15	that	that	WDT
cord-104268-q1jx0n0l	157	16	appears	appear	VBZ
cord-104268-q1jx0n0l	157	17	to	to	TO
cord-104268-q1jx0n0l	157	18	be	be	VB
cord-104268-q1jx0n0l	157	19	distinct	distinct	JJ
cord-104268-q1jx0n0l	157	20	from	from	IN
cord-104268-q1jx0n0l	157	21	a	a	DT
cord-104268-q1jx0n0l	157	22	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	157	23	early	early	JJ
cord-104268-q1jx0n0l	157	24	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	157	25	compartment	compartment	NN
cord-104268-q1jx0n0l	157	26	responsible	responsible	JJ
cord-104268-q1jx0n0l	157	27	for	for	IN
cord-104268-q1jx0n0l	157	28	cxl,6-mannosyl	cxl,6-mannosyl	NNP
cord-104268-q1jx0n0l	157	29	side	side	NN
cord-104268-q1jx0n0l	157	30	chains	chain	NNS
cord-104268-q1jx0n0l	157	31	elongation	elongation	NN
cord-104268-q1jx0n0l	158	1	(	(	-LRB-
cord-104268-q1jx0n0l	158	2	Gaynor	Gaynor	NNP
cord-104268-q1jx0n0l	158	3	et	et	NNP
cord-104268-q1jx0n0l	158	4	al	al	NNP
cord-104268-q1jx0n0l	158	5	.	.	NNP
cord-104268-q1jx0n0l	158	6	,	,	,
cord-104268-q1jx0n0l	158	7	1994	1994	CD
cord-104268-q1jx0n0l	158	8	)	)	-RRB-
cord-104268-q1jx0n0l	158	9	.	.	.
cord-104268-q1jx0n0l	159	1	The	the	DT
cord-104268-q1jx0n0l	159	2	~1,2-and	~1,2-and	NNP
cord-104268-q1jx0n0l	159	3	al,3mannosyl	al,3mannosyl	JJ
cord-104268-q1jx0n0l	159	4	side	side	NN
cord-104268-q1jx0n0l	159	5	chain	chain	NN
cord-104268-q1jx0n0l	159	6	modifications	modification	NNS
cord-104268-q1jx0n0l	159	7	are	be	VBP
cord-104268-q1jx0n0l	159	8	predicted	predict	VBN
cord-104268-q1jx0n0l	159	9	to	to	TO
cord-104268-q1jx0n0l	159	10	occur	occur	VB
cord-104268-q1jx0n0l	159	11	in	in	IN
cord-104268-q1jx0n0l	159	12	the	the	DT
cord-104268-q1jx0n0l	159	13	medial	medial	JJ
cord-104268-q1jx0n0l	159	14	-	-	HYPH
cord-104268-q1jx0n0l	159	15	Golgi	golgi	NN
cord-104268-q1jx0n0l	159	16	.	.	.
cord-104268-q1jx0n0l	160	1	The	the	DT
cord-104268-q1jx0n0l	160	2	possible	possible	JJ
cord-104268-q1jx0n0l	160	3	S.	S.	NNP
cord-104268-q1jx0n0l	160	4	cerevisiae	cerevisiae	NN
cord-104268-q1jx0n0l	160	5	N	n	CD
cord-104268-q1jx0n0l	160	6	-	-	HYPH
cord-104268-q1jx0n0l	160	7	linked	link	VBN
cord-104268-q1jx0n0l	160	8	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	160	9	structures	structure	NNS
cord-104268-q1jx0n0l	160	10	are	be	VBP
cord-104268-q1jx0n0l	160	11	shown	show	VBN
cord-104268-q1jx0n0l	160	12	(	(	-LRB-
cord-104268-q1jx0n0l	160	13	adapted	adapt	VBN
cord-104268-q1jx0n0l	160	14	from	from	IN
cord-104268-q1jx0n0l	160	15	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	160	16	,	,	,
cord-104268-q1jx0n0l	160	17	1990	1990	CD
cord-104268-q1jx0n0l	160	18	and	and	CC
cord-104268-q1jx0n0l	160	19	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	160	20	et	et	NNP
cord-104268-q1jx0n0l	160	21	al	al	NNP
cord-104268-q1jx0n0l	160	22	.	.	NNP
cord-104268-q1jx0n0l	160	23	,	,	,
cord-104268-q1jx0n0l	160	24	1990	1990	CD
cord-104268-q1jx0n0l	160	25	)	)	-RRB-
cord-104268-q1jx0n0l	160	26	.	.	.
cord-104268-q1jx0n0l	161	1	Arrows	arrow	NNS
cord-104268-q1jx0n0l	161	2	depict	depict	VBP
cord-104268-q1jx0n0l	161	3	[	[	-LRB-
cord-104268-q1jx0n0l	161	4	31,4	31,4	CD
cord-104268-q1jx0n0l	161	5	,	,	,
cord-104268-q1jx0n0l	161	6	al,6	al,6	NNP
cord-104268-q1jx0n0l	161	7	,	,	,
cord-104268-q1jx0n0l	161	8	cd,2	cd,2	UH
cord-104268-q1jx0n0l	161	9	,	,	,
cord-104268-q1jx0n0l	161	10	and	and	CC
cord-104268-q1jx0n0l	161	11	cd,3	cd,3	JJ
cord-104268-q1jx0n0l	161	12	linkages	linkage	NNS
cord-104268-q1jx0n0l	161	13	between	between	IN
cord-104268-q1jx0n0l	161	14	mannoses	mannose	NNS
cord-104268-q1jx0n0l	161	15	of	of	IN
cord-104268-q1jx0n0l	161	16	the	the	DT
cord-104268-q1jx0n0l	161	17	core	core	NN
cord-104268-q1jx0n0l	161	18	and	and	CC
cord-104268-q1jx0n0l	161	19	outer	outer	JJ
cord-104268-q1jx0n0l	161	20	chain	chain	NN
cord-104268-q1jx0n0l	161	21	.	.	.
cord-104268-q1jx0n0l	162	1	X	x	NN
cord-104268-q1jx0n0l	162	2	=	=	SYM
cord-104268-q1jx0n0l	162	3	10	10	CD
cord-104268-q1jx0n0l	162	4	on	on	IN
cord-104268-q1jx0n0l	162	5	average	average	JJ
cord-104268-q1jx0n0l	162	6	.	.	.
cord-104268-q1jx0n0l	163	1	The	the	DT
cord-104268-q1jx0n0l	163	2	mannose	mannose	NN
cord-104268-q1jx0n0l	163	3	units	unit	NNS
cord-104268-q1jx0n0l	163	4	not	not	RB
cord-104268-q1jx0n0l	163	5	present	present	JJ
cord-104268-q1jx0n0l	163	6	in	in	IN
cord-104268-q1jx0n0l	163	7	the	the	DT
cord-104268-q1jx0n0l	163	8	mnn9	mnn9	NNP
cord-104268-q1jx0n0l	163	9	mutation	mutation	NN
cord-104268-q1jx0n0l	163	10	are	be	VBP
cord-104268-q1jx0n0l	163	11	indicated	indicate	VBN
cord-104268-q1jx0n0l	163	12	.	.	.
cord-104268-q1jx0n0l	164	1	The	the	DT
cord-104268-q1jx0n0l	164	2	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	164	3	-	-	HYPH
cord-104268-q1jx0n0l	164	4	terminal	terminal	NN
cord-104268-q1jx0n0l	164	5	al,3-mannosyltransferase	al,3-mannosyltransferase	CC
cord-104268-q1jx0n0l	164	6	is	be	VBZ
cord-104268-q1jx0n0l	164	7	responsible	responsible	JJ
cord-104268-q1jx0n0l	164	8	for	for	IN
cord-104268-q1jx0n0l	164	9	the	the	DT
cord-104268-q1jx0n0l	164	10	addition	addition	NN
cord-104268-q1jx0n0l	164	11	of	of	IN
cord-104268-q1jx0n0l	164	12	all	all	DT
cord-104268-q1jx0n0l	164	13	al,3-mannosyl	al,3-mannosyl	NN
cord-104268-q1jx0n0l	164	14	residues	residue	NNS
cord-104268-q1jx0n0l	164	15	in	in	IN
cord-104268-q1jx0n0l	164	16	a	a	DT
cord-104268-q1jx0n0l	164	17	medial	medial	JJ
cord-104268-q1jx0n0l	164	18	-	-	HYPH
cord-104268-q1jx0n0l	164	19	Golgi	golgi	NN
cord-104268-q1jx0n0l	164	20	compartment	compartment	NN
cord-104268-q1jx0n0l	164	21	(	(	-LRB-
cord-104268-q1jx0n0l	164	22	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	164	23	,	,	,
cord-104268-q1jx0n0l	164	24	1990	1990	CD
cord-104268-q1jx0n0l	164	25	;	;	:
cord-104268-q1jx0n0l	164	26	Graham	Graham	NNP
cord-104268-q1jx0n0l	164	27	et	et	NNP
cord-104268-q1jx0n0l	164	28	al	al	NNP
cord-104268-q1jx0n0l	164	29	.	.	NNP
cord-104268-q1jx0n0l	164	30	,	,	,
cord-104268-q1jx0n0l	164	31	1994	1994	CD
cord-104268-q1jx0n0l	164	32	;	;	.
cord-104268-q1jx0n0l	165	1	Yip	Yip	NNP
cord-104268-q1jx0n0l	165	2	et	et	NNP
cord-104268-q1jx0n0l	165	3	al	al	NNP
cord-104268-q1jx0n0l	165	4	.	.	NNP
cord-104268-q1jx0n0l	165	5	,	,	,
cord-104268-q1jx0n0l	165	6	1994	1994	CD
cord-104268-q1jx0n0l	165	7	)	)	-RRB-
cord-104268-q1jx0n0l	165	8	.	.	.
cord-104268-q1jx0n0l	166	1	[	[	-LRB-
cord-104268-q1jx0n0l	166	2	]	]	-RRB-
cord-104268-q1jx0n0l	166	3	represents	represent	VBZ
cord-104268-q1jx0n0l	166	4	mannose	mannose	NN
cord-104268-q1jx0n0l	166	5	residues	residue	NNS
cord-104268-q1jx0n0l	166	6	that	that	WDT
cord-104268-q1jx0n0l	166	7	are	be	VBP
cord-104268-q1jx0n0l	166	8	thought	think	VBN
cord-104268-q1jx0n0l	166	9	to	to	TO
cord-104268-q1jx0n0l	166	10	be	be	VB
cord-104268-q1jx0n0l	166	11	added	add	VBN
cord-104268-q1jx0n0l	166	12	to	to	IN
cord-104268-q1jx0n0l	166	13	the	the	DT
cord-104268-q1jx0n0l	166	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	166	15	core	core	NN
cord-104268-q1jx0n0l	166	16	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	166	17	in	in	IN
cord-104268-q1jx0n0l	166	18	a	a	DT
cord-104268-q1jx0n0l	166	19	MNNl	mnnl	NN
cord-104268-q1jx0n0l	166	20	-	-	HYPH
cord-104268-q1jx0n0l	166	21	dependent	dependent	JJ
cord-104268-q1jx0n0l	166	22	manner	manner	NN
cord-104268-q1jx0n0l	166	23	(	(	-LRB-
cord-104268-q1jx0n0l	166	24	see	see	VB
cord-104268-q1jx0n0l	166	25	Fig	Fig	NNP
cord-104268-q1jx0n0l	166	26	.	.	.
cord-104268-q1jx0n0l	166	27	3	3	CD
cord-104268-q1jx0n0l	166	28	)	)	-RRB-
cord-104268-q1jx0n0l	166	29	.	.	.
cord-104268-q1jx0n0l	167	1	Fully	fully	RB
cord-104268-q1jx0n0l	167	2	glycosylated	glycosylated	JJ
cord-104268-q1jx0n0l	167	3	proteins	protein	NNS
cord-104268-q1jx0n0l	167	4	are	be	VBP
cord-104268-q1jx0n0l	167	5	then	then	RB
cord-104268-q1jx0n0l	167	6	transported	transport	VBN
cord-104268-q1jx0n0l	167	7	to	to	IN
cord-104268-q1jx0n0l	167	8	a	a	DT
cord-104268-q1jx0n0l	167	9	late	late	JJ
cord-104268-q1jx0n0l	167	10	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	167	11	compartment	compartment	NN
cord-104268-q1jx0n0l	167	12	which	which	WDT
cord-104268-q1jx0n0l	167	13	includes	include	VBZ
cord-104268-q1jx0n0l	167	14	the	the	DT
cord-104268-q1jx0n0l	167	15	proteolytic	proteolytic	JJ
cord-104268-q1jx0n0l	167	16	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	167	17	(	(	-LRB-
cord-104268-q1jx0n0l	167	18	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	167	19	,	,	,
cord-104268-q1jx0n0l	167	20	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	167	21	,	,	,
cord-104268-q1jx0n0l	167	22	and	and	CC
cord-104268-q1jx0n0l	167	23	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	167	24	A	A	NNP
cord-104268-q1jx0n0l	167	25	)	)	-RRB-
cord-104268-q1jx0n0l	167	26	responsible	responsible	JJ
cord-104268-q1jx0n0l	167	27	for	for	IN
cord-104268-q1jx0n0l	167	28	maturation	maturation	NN
cord-104268-q1jx0n0l	167	29	of	of	IN
cord-104268-q1jx0n0l	167	30	secreted	secrete	VBN
cord-104268-q1jx0n0l	167	31	protein	protein	NN
cord-104268-q1jx0n0l	167	32	precursors	precursor	NNS
cord-104268-q1jx0n0l	167	33	.	.	.
cord-104268-q1jx0n0l	168	1	Glycoproteins	glycoprotein	NNS
cord-104268-q1jx0n0l	168	2	that	that	WDT
cord-104268-q1jx0n0l	168	3	are	be	VBP
cord-104268-q1jx0n0l	168	4	not	not	RB
cord-104268-q1jx0n0l	168	5	retained	retain	VBN
cord-104268-q1jx0n0l	168	6	in	in	IN
cord-104268-q1jx0n0l	168	7	the	the	DT
cord-104268-q1jx0n0l	168	8	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	168	9	complex	complex	NN
cord-104268-q1jx0n0l	168	10	can	can	MD
cord-104268-q1jx0n0l	168	11	be	be	VB
cord-104268-q1jx0n0l	168	12	subsequently	subsequently	RB
cord-104268-q1jx0n0l	168	13	directed	direct	VBN
cord-104268-q1jx0n0l	168	14	to	to	IN
cord-104268-q1jx0n0l	168	15	the	the	DT
cord-104268-q1jx0n0l	168	16	vacuole	vacuole	NN
cord-104268-q1jx0n0l	168	17	through	through	IN
cord-104268-q1jx0n0l	168	18	an	an	DT
cord-104268-q1jx0n0l	168	19	endosomal	endosomal	NNP
cord-104268-q1jx0n0l	168	20	compartment	compartment	NN
cord-104268-q1jx0n0l	168	21	or	or	CC
cord-104268-q1jx0n0l	168	22	to	to	IN
cord-104268-q1jx0n0l	168	23	the	the	DT
cord-104268-q1jx0n0l	168	24	cell	cell	NN
cord-104268-q1jx0n0l	168	25	surface	surface	NN
cord-104268-q1jx0n0l	168	26	where	where	WRB
cord-104268-q1jx0n0l	168	27	they	-PRON-	PRP
cord-104268-q1jx0n0l	168	28	can	can	MD
cord-104268-q1jx0n0l	168	29	be	be	VB
cord-104268-q1jx0n0l	168	30	(	(	-LRB-
cord-104268-q1jx0n0l	168	31	a	a	LS
cord-104268-q1jx0n0l	168	32	)	)	-RRB-
cord-104268-q1jx0n0l	168	33	incorporated	incorporate	VBN
cord-104268-q1jx0n0l	168	34	into	into	IN
cord-104268-q1jx0n0l	168	35	the	the	DT
cord-104268-q1jx0n0l	168	36	plasma	plasma	NN
cord-104268-q1jx0n0l	168	37	membrane	membrane	NN
cord-104268-q1jx0n0l	168	38	,	,	,
cord-104268-q1jx0n0l	168	39	(	(	-LRB-
cord-104268-q1jx0n0l	168	40	b	b	NN
cord-104268-q1jx0n0l	168	41	)	)	-RRB-
cord-104268-q1jx0n0l	168	42	secreted	secrete	VBD
cord-104268-q1jx0n0l	168	43	and	and	CC
cord-104268-q1jx0n0l	168	44	retained	retain	VBN
cord-104268-q1jx0n0l	168	45	in	in	IN
cord-104268-q1jx0n0l	168	46	the	the	DT
cord-104268-q1jx0n0l	168	47	periplasmic	periplasmic	JJ
cord-104268-q1jx0n0l	168	48	space	space	NN
cord-104268-q1jx0n0l	168	49	/	/	SYM
cord-104268-q1jx0n0l	168	50	cell	cell	NN
cord-104268-q1jx0n0l	168	51	wall	wall	NN
cord-104268-q1jx0n0l	168	52	,	,	,
cord-104268-q1jx0n0l	168	53	or	or	CC
cord-104268-q1jx0n0l	168	54	(	(	-LRB-
cord-104268-q1jx0n0l	168	55	c	c	NN
cord-104268-q1jx0n0l	168	56	)	)	-RRB-
cord-104268-q1jx0n0l	168	57	secreted	secrete	VBN
cord-104268-q1jx0n0l	168	58	extracellularly	extracellularly	RB
cord-104268-q1jx0n0l	168	59	.	.	.
cord-104268-q1jx0n0l	168	60	 	 	_SP
cord-104268-q1jx0n0l	169	1	Both	both	DT
cord-104268-q1jx0n0l	169	2	the	the	DT
cord-104268-q1jx0n0l	169	3	function	function	NN
cord-104268-q1jx0n0l	169	4	of	of	IN
cord-104268-q1jx0n0l	169	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	169	6	as	as	IN
cord-104268-q1jx0n0l	169	7	a	a	DT
cord-104268-q1jx0n0l	169	8	txl,2-mannosyltransferase	txl,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	169	9	and	and	CC
cord-104268-q1jx0n0l	169	10	its	-PRON-	PRP$
cord-104268-q1jx0n0l	169	11	slow	slow	JJ
cord-104268-q1jx0n0l	169	12	receipt	receipt	NN
cord-104268-q1jx0n0l	169	13	of	of	IN
cord-104268-q1jx0n0l	169	14	MNNl	MNNl	NNS
cord-104268-q1jx0n0l	169	15	-	-	HYPH
cord-104268-q1jx0n0l	169	16	dependent	dependent	JJ
cord-104268-q1jx0n0l	169	17	modifications	modification	NNS
cord-104268-q1jx0n0l	169	18	imply	imply	VBP
cord-104268-q1jx0n0l	169	19	localization	localization	NN
cord-104268-q1jx0n0l	169	20	to	to	IN
cord-104268-q1jx0n0l	169	21	a	a	DT
cord-104268-q1jx0n0l	169	22	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	169	23	compartment	compartment	NN
cord-104268-q1jx0n0l	169	24	.	.	.
cord-104268-q1jx0n0l	170	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	170	2	subcellular	subcellular	JJ
cord-104268-q1jx0n0l	170	3	localization	localization	NN
cord-104268-q1jx0n0l	170	4	was	be	VBD
cord-104268-q1jx0n0l	170	5	examined	examine	VBN
cord-104268-q1jx0n0l	170	6	by	by	IN
cord-104268-q1jx0n0l	170	7	indirect	indirect	JJ
cord-104268-q1jx0n0l	170	8	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	170	9	and	and	CC
cord-104268-q1jx0n0l	170	10	showed	show	VBD
cord-104268-q1jx0n0l	170	11	a	a	DT
cord-104268-q1jx0n0l	170	12	punctate	punctate	NN
cord-104268-q1jx0n0l	170	13	pattern	pattern	NN
cord-104268-q1jx0n0l	170	14	of	of	IN
cord-104268-q1jx0n0l	170	15	fluorescent	fluorescent	NN
cord-104268-q1jx0n0l	170	16	signals	signal	NNS
cord-104268-q1jx0n0l	170	17	indicative	indicative	JJ
cord-104268-q1jx0n0l	170	18	of	of	IN
cord-104268-q1jx0n0l	170	19	a	a	DT
cord-104268-q1jx0n0l	170	20	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	170	21	association	association	NN
cord-104268-q1jx0n0l	170	22	(	(	-LRB-
cord-104268-q1jx0n0l	170	23	Fig	Fig	NNP
cord-104268-q1jx0n0l	170	24	.	.	.
cord-104268-q1jx0n0l	170	25	4	4	CD
cord-104268-q1jx0n0l	170	26	C	C	NNP
cord-104268-q1jx0n0l	170	27	)	)	-RRB-
cord-104268-q1jx0n0l	170	28	.	.	.
cord-104268-q1jx0n0l	171	1	Between	between	IN
cord-104268-q1jx0n0l	171	2	3	3	CD
cord-104268-q1jx0n0l	171	3	and	and	CC
cord-104268-q1jx0n0l	171	4	14	14	CD
cord-104268-q1jx0n0l	171	5	structures	structure	NNS
cord-104268-q1jx0n0l	171	6	per	per	IN
cord-104268-q1jx0n0l	171	7	cell	cell	NN
cord-104268-q1jx0n0l	171	8	can	can	MD
cord-104268-q1jx0n0l	171	9	be	be	VB
cord-104268-q1jx0n0l	171	10	seen	see	VBN
cord-104268-q1jx0n0l	171	11	,	,	,
cord-104268-q1jx0n0l	171	12	depending	depend	VBG
cord-104268-q1jx0n0l	171	13	on	on	IN
cord-104268-q1jx0n0l	171	14	individual	individual	JJ
cord-104268-q1jx0n0l	171	15	cells	cell	NNS
cord-104268-q1jx0n0l	171	16	and	and	CC
cord-104268-q1jx0n0l	171	17	the	the	DT
cord-104268-q1jx0n0l	171	18	plane	plane	NN
cord-104268-q1jx0n0l	171	19	of	of	IN
cord-104268-q1jx0n0l	171	20	focus	focus	NN
cord-104268-q1jx0n0l	171	21	.	.	.
cord-104268-q1jx0n0l	172	1	Punctiform	punctiform	JJ
cord-104268-q1jx0n0l	172	2	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	172	3	was	be	VBD
cord-104268-q1jx0n0l	172	4	never	never	RB
cord-104268-q1jx0n0l	172	5	seen	see	VBN
cord-104268-q1jx0n0l	172	6	with	with	IN
cord-104268-q1jx0n0l	172	7	the	the	DT
cord-104268-q1jx0n0l	172	8	anti	anti	JJ
cord-104268-q1jx0n0l	172	9	-	-	JJ
cord-104268-q1jx0n0l	172	10	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	172	11	antibody	antibody	NN
cord-104268-q1jx0n0l	172	12	in	in	IN
cord-104268-q1jx0n0l	172	13	kre2	kre2	NNP
cord-104268-q1jx0n0l	172	14	:	:	:
cord-104268-q1jx0n0l	172	15	:	:	:
cord-104268-q1jx0n0l	172	16	TRP1	TRP1	NNP
cord-104268-q1jx0n0l	172	17	cells	cell	NNS
cord-104268-q1jx0n0l	172	18	(	(	-LRB-
cord-104268-q1jx0n0l	172	19	Fig	fig	NN
cord-104268-q1jx0n0l	172	20	.	.	.
cord-104268-q1jx0n0l	172	21	4	4	CD
cord-104268-q1jx0n0l	172	22	A	a	NN
cord-104268-q1jx0n0l	172	23	)	)	-RRB-
cord-104268-q1jx0n0l	172	24	.	.	.
cord-104268-q1jx0n0l	173	1	The	the	DT
cord-104268-q1jx0n0l	173	2	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	173	3	signal	signal	NN
cord-104268-q1jx0n0l	173	4	distribution	distribution	NN
cord-104268-q1jx0n0l	173	5	did	do	VBD
cord-104268-q1jx0n0l	173	6	not	not	RB
cord-104268-q1jx0n0l	173	7	overlap	overlap	VB
cord-104268-q1jx0n0l	173	8	with	with	IN
cord-104268-q1jx0n0l	173	9	nuclei	nucleus	NNS
cord-104268-q1jx0n0l	173	10	or	or	CC
cord-104268-q1jx0n0l	173	11	mitochondria	mitochondrion	NNS
cord-104268-q1jx0n0l	173	12	viewed	view	VBN
cord-104268-q1jx0n0l	173	13	by	by	IN
cord-104268-q1jx0n0l	173	14	D	D	NNP
cord-104268-q1jx0n0l	173	15	N	N	NNP
cord-104268-q1jx0n0l	173	16	A	a	DT
cord-104268-q1jx0n0l	173	17	staining	staining	NN
cord-104268-q1jx0n0l	173	18	with	with	IN
cord-104268-q1jx0n0l	173	19	DAPI	dapi	FW
cord-104268-q1jx0n0l	173	20	(	(	-LRB-
cord-104268-q1jx0n0l	173	21	Fig	Fig	NNP
cord-104268-q1jx0n0l	173	22	.	.	.
cord-104268-q1jx0n0l	173	23	4	4	CD
cord-104268-q1jx0n0l	173	24	D	d	NN
cord-104268-q1jx0n0l	173	25	)	)	-RRB-
cord-104268-q1jx0n0l	173	26	.	.	.
cord-104268-q1jx0n0l	174	1	To	to	TO
cord-104268-q1jx0n0l	174	2	attempt	attempt	VB
cord-104268-q1jx0n0l	174	3	to	to	TO
cord-104268-q1jx0n0l	174	4	define	define	VB
cord-104268-q1jx0n0l	174	5	in	in	IN
cord-104268-q1jx0n0l	174	6	which	which	WDT
cord-104268-q1jx0n0l	174	7	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	174	8	compartment	compartment	NN
cord-104268-q1jx0n0l	174	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	174	10	is	be	VBZ
cord-104268-q1jx0n0l	174	11	localized	localize	VBN
cord-104268-q1jx0n0l	174	12	,	,	,
cord-104268-q1jx0n0l	174	13	its	-PRON-	PRP$
cord-104268-q1jx0n0l	174	14	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	174	15	localization	localization	NN
cord-104268-q1jx0n0l	174	16	was	be	VBD
cord-104268-q1jx0n0l	174	17	compared	compare	VBN
cord-104268-q1jx0n0l	174	18	with	with	IN
cord-104268-q1jx0n0l	174	19	that	that	DT
cord-104268-q1jx0n0l	174	20	of	of	IN
cord-104268-q1jx0n0l	174	21	a	a	DT
cord-104268-q1jx0n0l	174	22	known	know	VBN
cord-104268-q1jx0n0l	174	23	medial	medial	JJ
cord-104268-q1jx0n0l	174	24	-	-	HYPH
cord-104268-q1jx0n0l	174	25	Golgi	golgi	NN
cord-104268-q1jx0n0l	174	26	protein	protein	NN
cord-104268-q1jx0n0l	174	27	,	,	,
cord-104268-q1jx0n0l	174	28	the	the	DT
cord-104268-q1jx0n0l	174	29	mannosyltransferase	mannosyltransferase	NNP
cord-104268-q1jx0n0l	174	30	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	174	31	(	(	-LRB-
cord-104268-q1jx0n0l	174	32	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	174	33	,	,	,
cord-104268-q1jx0n0l	174	34	1990	1990	CD
cord-104268-q1jx0n0l	174	35	;	;	:
cord-104268-q1jx0n0l	174	36	Graham	Graham	NNP
cord-104268-q1jx0n0l	174	37	et	et	NNP
cord-104268-q1jx0n0l	174	38	al	al	NNP
cord-104268-q1jx0n0l	174	39	.	.	NNP
cord-104268-q1jx0n0l	174	40	,	,	,
cord-104268-q1jx0n0l	174	41	1994	1994	CD
cord-104268-q1jx0n0l	174	42	;	;	:
cord-104268-q1jx0n0l	174	43	Yip	Yip	NNP
cord-104268-q1jx0n0l	174	44	et	et	NNP
cord-104268-q1jx0n0l	174	45	al	al	NNP
cord-104268-q1jx0n0l	174	46	.	.	NNP
cord-104268-q1jx0n0l	174	47	,	,	,
cord-104268-q1jx0n0l	174	48	1994	1994	CD
cord-104268-q1jx0n0l	174	49	)	)	-RRB-
cord-104268-q1jx0n0l	174	50	,	,	,
cord-104268-q1jx0n0l	174	51	by	by	IN
cord-104268-q1jx0n0l	174	52	double	double	JJ
cord-104268-q1jx0n0l	174	53	-	-	HYPH
cord-104268-q1jx0n0l	174	54	label	label	NN
cord-104268-q1jx0n0l	174	55	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	174	56	(	(	-LRB-
cord-104268-q1jx0n0l	174	57	Fig	Fig	NNP
cord-104268-q1jx0n0l	174	58	.	.	.
cord-104268-q1jx0n0l	174	59	5	5	CD
cord-104268-q1jx0n0l	174	60	)	)	-RRB-
cord-104268-q1jx0n0l	174	61	.	.	.
cord-104268-q1jx0n0l	175	1	Quantitative	quantitative	JJ
cord-104268-q1jx0n0l	175	2	evaluation	evaluation	NN
cord-104268-q1jx0n0l	175	3	of	of	IN
cord-104268-q1jx0n0l	175	4	punctate	punctate	NN
cord-104268-q1jx0n0l	175	5	spot	spot	NN
cord-104268-q1jx0n0l	175	6	distribution	distribution	NN
cord-104268-q1jx0n0l	175	7	(	(	-LRB-
cord-104268-q1jx0n0l	175	8	described	describe	VBN
cord-104268-q1jx0n0l	175	9	in	in	IN
cord-104268-q1jx0n0l	175	10	Materials	material	NNS
cord-104268-q1jx0n0l	175	11	and	and	CC
cord-104268-q1jx0n0l	175	12	Methods	Methods	NNPS
cord-104268-q1jx0n0l	175	13	)	)	-RRB-
cord-104268-q1jx0n0l	175	14	revealed	reveal	VBD
cord-104268-q1jx0n0l	175	15	that	that	IN
cord-104268-q1jx0n0l	175	16	75	75	CD
cord-104268-q1jx0n0l	175	17	%	%	NN
cord-104268-q1jx0n0l	175	18	of	of	IN
cord-104268-q1jx0n0l	175	19	the	the	DT
cord-104268-q1jx0n0l	175	20	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	175	21	corresponding	correspond	VBG
cord-104268-q1jx0n0l	175	22	Texas	Texas	NNP
cord-104268-q1jx0n0l	175	23	red	red	RB
cord-104268-q1jx0n0l	175	24	-	-	HYPH
cord-104268-q1jx0n0l	175	25	labeled	label	VBN
cord-104268-q1jx0n0l	175	26	structures	structure	NNS
cord-104268-q1jx0n0l	175	27	coincided	coincide	VBD
cord-104268-q1jx0n0l	175	28	with	with	IN
cord-104268-q1jx0n0l	175	29	the	the	DT
cord-104268-q1jx0n0l	175	30	FITC	FITC	NNP
cord-104268-q1jx0n0l	175	31	-	-	HYPH
cord-104268-q1jx0n0l	175	32	labeled	label	VBN
cord-104268-q1jx0n0l	175	33	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	175	34	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	175	35	spots	spot	NNS
cord-104268-q1jx0n0l	175	36	.	.	.
cord-104268-q1jx0n0l	176	1	The	the	DT
cord-104268-q1jx0n0l	176	2	location	location	NN
cord-104268-q1jx0n0l	176	3	of	of	IN
cord-104268-q1jx0n0l	176	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	176	5	was	be	VBD
cord-104268-q1jx0n0l	176	6	also	also	RB
cord-104268-q1jx0n0l	176	7	compared	compare	VBN
cord-104268-q1jx0n0l	176	8	with	with	IN
cord-104268-q1jx0n0l	176	9	that	that	DT
cord-104268-q1jx0n0l	176	10	of	of	IN
cord-104268-q1jx0n0l	176	11	a	a	DT
cord-104268-q1jx0n0l	176	12	late	late	JJ
cord-104268-q1jx0n0l	176	13	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	176	14	protein	protein	NN
cord-104268-q1jx0n0l	176	15	,	,	,
cord-104268-q1jx0n0l	176	16	the	the	DT
cord-104268-q1jx0n0l	176	17	carboxypeptidase	carboxypeptidase	NN
cord-104268-q1jx0n0l	176	18	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	176	19	(	(	-LRB-
cord-104268-q1jx0n0l	176	20	see	see	VB
cord-104268-q1jx0n0l	176	21	Figs	fig	NNS
cord-104268-q1jx0n0l	176	22	.	.	.
cord-104268-q1jx0n0l	177	1	2	2	CD
cord-104268-q1jx0n0l	177	2	and	and	CC
cord-104268-q1jx0n0l	177	3	5	5	CD
cord-104268-q1jx0n0l	177	4	;	;	:
cord-104268-q1jx0n0l	177	5	Dmochowska	Dmochowska	NNP
cord-104268-q1jx0n0l	177	6	et	et	NNP
cord-104268-q1jx0n0l	177	7	al	al	NNP
cord-104268-q1jx0n0l	177	8	.	.	NNP
cord-104268-q1jx0n0l	177	9	,	,	,
cord-104268-q1jx0n0l	177	10	1987	1987	CD
cord-104268-q1jx0n0l	177	11	;	;	:
cord-104268-q1jx0n0l	177	12	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	177	13	,	,	,
cord-104268-q1jx0n0l	177	14	1989	1989	CD
cord-104268-q1jx0n0l	177	15	,	,	,
cord-104268-q1jx0n0l	177	16	1992	1992	CD
cord-104268-q1jx0n0l	177	17	)	)	-RRB-
cord-104268-q1jx0n0l	177	18	.	.	.
cord-104268-q1jx0n0l	178	1	A	a	DT
cord-104268-q1jx0n0l	178	2	quantitative	quantitative	JJ
cord-104268-q1jx0n0l	178	3	scoring	scoring	NN
cord-104268-q1jx0n0l	178	4	of	of	IN
cord-104268-q1jx0n0l	178	5	punctiform	punctiform	NN
cord-104268-q1jx0n0l	178	6	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	178	7	distribution	distribution	NN
cord-104268-q1jx0n0l	178	8	indicated	indicate	VBD
cord-104268-q1jx0n0l	178	9	that	that	IN
cord-104268-q1jx0n0l	178	10	65	65	CD
cord-104268-q1jx0n0l	178	11	%	%	NN
cord-104268-q1jx0n0l	178	12	of	of	IN
cord-104268-q1jx0n0l	178	13	the	the	DT
cord-104268-q1jx0n0l	178	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	178	15	corresponding	correspond	VBG
cord-104268-q1jx0n0l	178	16	structures	structure	NNS
cord-104268-q1jx0n0l	178	17	did	do	VBD
cord-104268-q1jx0n0l	178	18	not	not	RB
cord-104268-q1jx0n0l	178	19	coincide	coincide	VB
cord-104268-q1jx0n0l	178	20	with	with	IN
cord-104268-q1jx0n0l	178	21	the	the	DT
cord-104268-q1jx0n0l	178	22	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	178	23	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	178	24	patches	patch	NNS
cord-104268-q1jx0n0l	178	25	.	.	.
cord-104268-q1jx0n0l	179	1	To	to	TO
cord-104268-q1jx0n0l	179	2	examine	examine	VB
cord-104268-q1jx0n0l	179	3	the	the	DT
cord-104268-q1jx0n0l	179	4	basis	basis	NN
cord-104268-q1jx0n0l	179	5	of	of	IN
cord-104268-q1jx0n0l	179	6	localization	localization	NN
cord-104268-q1jx0n0l	179	7	of	of	IN
cord-104268-q1jx0n0l	179	8	a	a	DT
cord-104268-q1jx0n0l	179	9	yeast	yeast	NN
cord-104268-q1jx0n0l	179	10	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	179	11	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	179	12	,	,	,
cord-104268-q1jx0n0l	179	13	an	an	DT
cord-104268-q1jx0n0l	179	14	analysis	analysis	NN
cord-104268-q1jx0n0l	179	15	of	of	IN
cord-104268-q1jx0n0l	179	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	179	17	noncatalytic	noncatalytic	JJ
cord-104268-q1jx0n0l	179	18	domains	domain	NNS
cord-104268-q1jx0n0l	179	19	was	be	VBD
cord-104268-q1jx0n0l	179	20	made	make	VBN
cord-104268-q1jx0n0l	179	21	.	.	.
cord-104268-q1jx0n0l	180	1	The	the	DT
cord-104268-q1jx0n0l	180	2	roles	role	NNS
cord-104268-q1jx0n0l	180	3	of	of	IN
cord-104268-q1jx0n0l	180	4	the	the	DT
cord-104268-q1jx0n0l	180	5	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	180	6	NH2	NH2	NNP
cord-104268-q1jx0n0l	180	7	terminus	terminus	NN
cord-104268-q1jx0n0l	180	8	,	,	,
cord-104268-q1jx0n0l	180	9	TMD	TMD	NNP
cord-104268-q1jx0n0l	180	10	,	,	,
cord-104268-q1jx0n0l	180	11	and	and	CC
cord-104268-q1jx0n0l	180	12	luminal	luminal	JJ
cord-104268-q1jx0n0l	180	13	stem	stem	NN
cord-104268-q1jx0n0l	180	14	region	region	NN
cord-104268-q1jx0n0l	180	15	of	of	IN
cord-104268-q1jx0n0l	180	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	180	17	in	in	IN
cord-104268-q1jx0n0l	180	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	180	19	targeting	target	VBG
cord-104268-q1jx0n0l	180	20	were	be	VBD
cord-104268-q1jx0n0l	180	21	tested	test	VBN
cord-104268-q1jx0n0l	180	22	by	by	IN
cord-104268-q1jx0n0l	180	23	constructing	construct	VBG
cord-104268-q1jx0n0l	180	24	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	180	25	proteins	protein	NNS
cord-104268-q1jx0n0l	180	26	in	in	IN
cord-104268-q1jx0n0l	180	27	which	which	WDT
cord-104268-q1jx0n0l	180	28	Kre2p	Kre2p	VBN
cord-104268-q1jx0n0l	180	29	-	-	HYPH
cord-104268-q1jx0n0l	180	30	specific	specific	JJ
cord-104268-q1jx0n0l	180	31	segments	segment	NNS
cord-104268-q1jx0n0l	180	32	were	be	VBD
cord-104268-q1jx0n0l	180	33	substituted	substitute	VBN
cord-104268-q1jx0n0l	180	34	with	with	IN
cord-104268-q1jx0n0l	180	35	the	the	DT
cord-104268-q1jx0n0l	180	36	corresponding	correspond	VBG
cord-104268-q1jx0n0l	180	37	domains	domain	NNS
cord-104268-q1jx0n0l	180	38	of	of	IN
cord-104268-q1jx0n0l	180	39	the	the	DT
cord-104268-q1jx0n0l	180	40	DAP2	DAP2	NNP
cord-104268-q1jx0n0l	180	41	or	or	CC
cord-104268-q1jx0n0l	180	42	PH08	PH08	NNP
cord-104268-q1jx0n0l	180	43	gene	gene	NN
cord-104268-q1jx0n0l	180	44	products	product	NNS
cord-104268-q1jx0n0l	180	45	(	(	-LRB-
cord-104268-q1jx0n0l	180	46	Fig	Fig	NNP
cord-104268-q1jx0n0l	180	47	.	.	.
cord-104268-q1jx0n0l	180	48	6	6	CD
cord-104268-q1jx0n0l	180	49	)	)	-RRB-
cord-104268-q1jx0n0l	180	50	.	.	.
cord-104268-q1jx0n0l	181	1	DAP2	DAP2	NNP
cord-104268-q1jx0n0l	181	2	encodes	encode	VBZ
cord-104268-q1jx0n0l	181	3	the	the	DT
cord-104268-q1jx0n0l	181	4	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	181	5	dipeptidyl	dipeptidyl	NNP
cord-104268-q1jx0n0l	181	6	aminopeptidase	aminopeptidase	NN
cord-104268-q1jx0n0l	181	7	B	B	NNP
cord-104268-q1jx0n0l	181	8	(	(	-LRB-
cord-104268-q1jx0n0l	181	9	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	181	10	et	et	NNP
cord-104268-q1jx0n0l	181	11	al	al	NNP
cord-104268-q1jx0n0l	181	12	.	.	NNP
cord-104268-q1jx0n0l	181	13	,	,	,
cord-104268-q1jx0n0l	181	14	1989	1989	CD
cord-104268-q1jx0n0l	181	15	)	)	-RRB-
cord-104268-q1jx0n0l	181	16	,	,	,
cord-104268-q1jx0n0l	181	17	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	181	18	B	B	NNP
cord-104268-q1jx0n0l	181	19	,	,	,
cord-104268-q1jx0n0l	181	20	and	and	CC
cord-104268-q1jx0n0l	181	21	is	be	VBZ
cord-104268-q1jx0n0l	181	22	a	a	DT
cord-104268-q1jx0n0l	181	23	type	type	NN
cord-104268-q1jx0n0l	181	24	II	ii	CD
cord-104268-q1jx0n0l	181	25	integral	integral	JJ
cord-104268-q1jx0n0l	181	26	membrane	membrane	NN
cord-104268-q1jx0n0l	181	27	glycoprotein	glycoprotein	NN
cord-104268-q1jx0n0l	181	28	that	that	WDT
cord-104268-q1jx0n0l	181	29	lacks	lack	VBZ
cord-104268-q1jx0n0l	181	30	apparent	apparent	JJ
cord-104268-q1jx0n0l	181	31	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	181	32	targeting	target	VBG
cord-104268-q1jx0n0l	181	33	information	information	NN
cord-104268-q1jx0n0l	181	34	.	.	.
cord-104268-q1jx0n0l	182	1	No	no	DT
cord-104268-q1jx0n0l	182	2	individual	individual	JJ
cord-104268-q1jx0n0l	182	3	domain	domain	NN
cord-104268-q1jx0n0l	182	4	of	of	IN
cord-104268-q1jx0n0l	182	5	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	182	6	B	B	NNP
cord-104268-q1jx0n0l	182	7	was	be	VBD
cord-104268-q1jx0n0l	182	8	shown	show	VBN
cord-104268-q1jx0n0l	182	9	to	to	TO
cord-104268-q1jx0n0l	182	10	be	be	VB
cord-104268-q1jx0n0l	182	11	required	require	VBN
cord-104268-q1jx0n0l	182	12	for	for	IN
cord-104268-q1jx0n0l	182	13	its	-PRON-	PRP$
cord-104268-q1jx0n0l	182	14	transport	transport	NN
cord-104268-q1jx0n0l	182	15	to	to	IN
cord-104268-q1jx0n0l	182	16	the	the	DT
cord-104268-q1jx0n0l	182	17	vacuole	vacuole	NN
cord-104268-q1jx0n0l	182	18	besides	besides	IN
cord-104268-q1jx0n0l	182	19	a	a	DT
cord-104268-q1jx0n0l	182	20	nonspecific	nonspecific	JJ
cord-104268-q1jx0n0l	182	21	hydrophobic	hydrophobic	JJ
cord-104268-q1jx0n0l	182	22	TMD	TMD	NNP
cord-104268-q1jx0n0l	182	23	(	(	-LRB-
cord-104268-q1jx0n0l	182	24	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	182	25	et	et	NNP
cord-104268-q1jx0n0l	182	26	al	al	NNP
cord-104268-q1jx0n0l	182	27	.	.	NNP
cord-104268-q1jx0n0l	182	28	,	,	,
cord-104268-q1jx0n0l	182	29	1992	1992	CD
cord-104268-q1jx0n0l	182	30	)	)	-RRB-
cord-104268-q1jx0n0l	182	31	.	.	.
cord-104268-q1jx0n0l	183	1	PH08	PH08	NNP
cord-104268-q1jx0n0l	183	2	encodes	encode	VBZ
cord-104268-q1jx0n0l	183	3	a	a	DT
cord-104268-q1jx0n0l	183	4	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	183	5	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	183	6	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	183	7	.	.	.
cord-104268-q1jx0n0l	184	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	184	2	is	be	VBZ
cord-104268-q1jx0n0l	184	3	also	also	RB
cord-104268-q1jx0n0l	184	4	a	a	DT
cord-104268-q1jx0n0l	184	5	type	type	NN
cord-104268-q1jx0n0l	184	6	II	II	NNP
cord-104268-q1jx0n0l	184	7	membrane	membrane	NN
cord-104268-q1jx0n0l	184	8	protein	protein	NN
cord-104268-q1jx0n0l	184	9	(	(	-LRB-
cord-104268-q1jx0n0l	184	10	Kaneko	Kaneko	NNP
cord-104268-q1jx0n0l	184	11	et	et	NNP
cord-104268-q1jx0n0l	184	12	al	al	NNP
cord-104268-q1jx0n0l	184	13	.	.	NNP
cord-104268-q1jx0n0l	184	14	,	,	,
cord-104268-q1jx0n0l	184	15	1987	1987	CD
cord-104268-q1jx0n0l	184	16	;	;	:
cord-104268-q1jx0n0l	184	17	Klionsky	Klionsky	NNP
cord-104268-q1jx0n0l	184	18	and	and	CC
cord-104268-q1jx0n0l	184	19	Emr	Emr	NNP
cord-104268-q1jx0n0l	184	20	,	,	,
cord-104268-q1jx0n0l	184	21	1989	1989	CD
cord-104268-q1jx0n0l	184	22	;	;	:
cord-104268-q1jx0n0l	185	1	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	185	2	et	et	FW
cord-104268-q1jx0n0l	185	3	al	al	NNP
cord-104268-q1jx0n0l	185	4	.	.	NNP
cord-104268-q1jx0n0l	185	5	,	,	,
cord-104268-q1jx0n0l	185	6	1993	1993	CD
cord-104268-q1jx0n0l	185	7	)	)	-RRB-
cord-104268-q1jx0n0l	185	8	that	that	WDT
cord-104268-q1jx0n0l	185	9	is	be	VBZ
cord-104268-q1jx0n0l	185	10	thought	think	VBN
cord-104268-q1jx0n0l	185	11	to	to	TO
cord-104268-q1jx0n0l	185	12	be	be	VB
cord-104268-q1jx0n0l	185	13	transported	transport	VBN
cord-104268-q1jx0n0l	185	14	to	to	IN
cord-104268-q1jx0n0l	185	15	the	the	DT
cord-104268-q1jx0n0l	185	16	vacuole	vacuole	NN
cord-104268-q1jx0n0l	185	17	by	by	IN
cord-104268-q1jx0n0l	185	18	default	default	NN
cord-104268-q1jx0n0l	185	19	(	(	-LRB-
cord-104268-q1jx0n0l	185	20	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	185	21	et	et	FW
cord-104268-q1jx0n0l	185	22	al	al	NNP
cord-104268-q1jx0n0l	185	23	.	.	NNP
cord-104268-q1jx0n0l	185	24	,	,	,
cord-104268-q1jx0n0l	185	25	1993	1993	CD
cord-104268-q1jx0n0l	185	26	)	)	-RRB-
cord-104268-q1jx0n0l	185	27	.	.	.
cord-104268-q1jx0n0l	186	1	Substitutions	substitution	NNS
cord-104268-q1jx0n0l	186	2	designed	design	VBN
cord-104268-q1jx0n0l	186	3	with	with	IN
cord-104268-q1jx0n0l	186	4	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	186	5	proteins	protein	NNS
cord-104268-q1jx0n0l	186	6	were	be	VBD
cord-104268-q1jx0n0l	186	7	used	use	VBN
cord-104268-q1jx0n0l	186	8	to	to	TO
cord-104268-q1jx0n0l	186	9	avoid	avoid	VB
cord-104268-q1jx0n0l	186	10	potential	potential	JJ
cord-104268-q1jx0n0l	186	11	problems	problem	NNS
cord-104268-q1jx0n0l	186	12	with	with	IN
cord-104268-q1jx0n0l	186	13	cryptic	cryptic	JJ
cord-104268-q1jx0n0l	186	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	186	15	-	-	HYPH
cord-104268-q1jx0n0l	186	16	targeting	target	VBG
cord-104268-q1jx0n0l	186	17	sequences	sequence	NNS
cord-104268-q1jx0n0l	186	18	.	.	.
cord-104268-q1jx0n0l	187	1	Indirect	indirect	JJ
cord-104268-q1jx0n0l	187	2	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	187	3	detection	detection	NN
cord-104268-q1jx0n0l	187	4	of	of	IN
cord-104268-q1jx0n0l	187	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	187	6	proteins	protein	NNS
cord-104268-q1jx0n0l	187	7	was	be	VBD
cord-104268-q1jx0n0l	187	8	undertaken	undertake	VBN
cord-104268-q1jx0n0l	187	9	to	to	TO
cord-104268-q1jx0n0l	187	10	determine	determine	VB
cord-104268-q1jx0n0l	187	11	their	-PRON-	PRP$
cord-104268-q1jx0n0l	187	12	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	187	13	location(s	location(s	NNP
cord-104268-q1jx0n0l	187	14	)	)	-RRB-
cord-104268-q1jx0n0l	187	15	.	.	.
cord-104268-q1jx0n0l	188	1	Initially	initially	RB
cord-104268-q1jx0n0l	188	2	,	,	,
cord-104268-q1jx0n0l	188	3	the	the	DT
cord-104268-q1jx0n0l	188	4	role	role	NN
cord-104268-q1jx0n0l	188	5	of	of	IN
cord-104268-q1jx0n0l	188	6	the	the	DT
cord-104268-q1jx0n0l	188	7	membrane	membrane	NN
cord-104268-q1jx0n0l	188	8	-	-	HYPH
cord-104268-q1jx0n0l	188	9	spanning	span	VBG
cord-104268-q1jx0n0l	188	10	domain	domain	NN
cord-104268-q1jx0n0l	188	11	of	of	IN
cord-104268-q1jx0n0l	188	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	188	13	was	be	VBD
cord-104268-q1jx0n0l	188	14	assessed	assess	VBN
cord-104268-q1jx0n0l	188	15	with	with	IN
cord-104268-q1jx0n0l	188	16	fusion	fusion	NN
cord-104268-q1jx0n0l	188	17	protein	protein	NN
cord-104268-q1jx0n0l	188	18	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	188	19	(	(	-LRB-
cord-104268-q1jx0n0l	188	20	Fig	Fig	NNP
cord-104268-q1jx0n0l	188	21	.	.	.
cord-104268-q1jx0n0l	188	22	6	6	CD
cord-104268-q1jx0n0l	188	23	)	)	-RRB-
cord-104268-q1jx0n0l	188	24	,	,	,
cord-104268-q1jx0n0l	188	25	in	in	IN
cord-104268-q1jx0n0l	188	26	which	which	WDT
cord-104268-q1jx0n0l	188	27	the	the	DT
cord-104268-q1jx0n0l	188	28	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	188	29	TMD	TMD	NNP
cord-104268-q1jx0n0l	188	30	was	be	VBD
cord-104268-q1jx0n0l	188	31	removed	remove	VBN
cord-104268-q1jx0n0l	188	32	and	and	CC
cord-104268-q1jx0n0l	188	33	substituted	substitute	VBN
cord-104268-q1jx0n0l	188	34	with	with	IN
cord-104268-q1jx0n0l	188	35	that	that	DT
cord-104268-q1jx0n0l	188	36	of	of	IN
cord-104268-q1jx0n0l	188	37	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	188	38	B.	B.	NNP
cord-104268-q1jx0n0l	188	39	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	188	40	was	be	VBD
cord-104268-q1jx0n0l	188	41	found	find	VBN
cord-104268-q1jx0n0l	188	42	,	,	,
cord-104268-q1jx0n0l	188	43	like	like	IN
cord-104268-q1jx0n0l	188	44	wild	wild	JJ
cord-104268-q1jx0n0l	188	45	-	-	HYPH
cord-104268-q1jx0n0l	188	46	type	type	NN
cord-104268-q1jx0n0l	188	47	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	188	48	,	,	,
cord-104268-q1jx0n0l	188	49	to	to	TO
cord-104268-q1jx0n0l	188	50	be	be	VB
cord-104268-q1jx0n0l	188	51	localized	localize	VBN
cord-104268-q1jx0n0l	188	52	to	to	IN
cord-104268-q1jx0n0l	188	53	small	small	JJ
cord-104268-q1jx0n0l	188	54	punctate	punctate	NN
cord-104268-q1jx0n0l	188	55	structures	structure	NNS
cord-104268-q1jx0n0l	188	56	of	of	IN
cord-104268-q1jx0n0l	188	57	the	the	DT
cord-104268-q1jx0n0l	188	58	yeast	yeast	NN
cord-104268-q1jx0n0l	188	59	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	188	60	(	(	-LRB-
cord-104268-q1jx0n0l	188	61	Fig	Fig	NNP
cord-104268-q1jx0n0l	188	62	.	.	NNP
cord-104268-q1jx0n0l	188	63	7	7	CD
cord-104268-q1jx0n0l	188	64	)	)	-RRB-
cord-104268-q1jx0n0l	188	65	.	.	.
cord-104268-q1jx0n0l	189	1	To	to	TO
cord-104268-q1jx0n0l	189	2	exclude	exclude	VB
cord-104268-q1jx0n0l	189	3	the	the	DT
cord-104268-q1jx0n0l	189	4	possibility	possibility	NN
cord-104268-q1jx0n0l	189	5	that	that	IN
cord-104268-q1jx0n0l	189	6	the	the	DT
cord-104268-q1jx0n0l	189	7	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	189	8	B	B	NNP
cord-104268-q1jx0n0l	189	9	TMD	TMD	NNP
cord-104268-q1jx0n0l	189	10	is	be	VBZ
cord-104268-q1jx0n0l	189	11	able	able	JJ
cord-104268-q1jx0n0l	189	12	to	to	TO
cord-104268-q1jx0n0l	189	13	function	function	VB
cord-104268-q1jx0n0l	189	14	as	as	IN
cord-104268-q1jx0n0l	189	15	a	a	DT
cord-104268-q1jx0n0l	189	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	189	17	TMD	TMD	NNP
cord-104268-q1jx0n0l	189	18	in	in	IN
cord-104268-q1jx0n0l	189	19	the	the	DT
cord-104268-q1jx0n0l	189	20	context	context	NN
cord-104268-q1jx0n0l	189	21	of	of	IN
cord-104268-q1jx0n0l	189	22	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	189	23	protein	protein	NN
cord-104268-q1jx0n0l	189	24	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	189	25	by	by	IN
cord-104268-q1jx0n0l	189	26	sharing	share	VBG
cord-104268-q1jx0n0l	189	27	some	some	DT
cord-104268-q1jx0n0l	189	28	unknown	unknown	JJ
cord-104268-q1jx0n0l	189	29	common	common	JJ
cord-104268-q1jx0n0l	189	30	features	feature	NNS
cord-104268-q1jx0n0l	189	31	not	not	RB
cord-104268-q1jx0n0l	189	32	apparent	apparent	JJ
cord-104268-q1jx0n0l	189	33	at	at	IN
cord-104268-q1jx0n0l	189	34	the	the	DT
cord-104268-q1jx0n0l	189	35	amino	amino	NN
cord-104268-q1jx0n0l	189	36	acid	acid	NN
cord-104268-q1jx0n0l	189	37	level	level	NN
cord-104268-q1jx0n0l	189	38	,	,	,
cord-104268-q1jx0n0l	189	39	a	a	DT
cord-104268-q1jx0n0l	189	40	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	189	41	protein	protein	NN
cord-104268-q1jx0n0l	189	42	consisting	consist	VBG
cord-104268-q1jx0n0l	189	43	of	of	IN
cord-104268-q1jx0n0l	189	44	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	189	45	with	with	IN
cord-104268-q1jx0n0l	189	46	the	the	DT
cord-104268-q1jx0n0l	189	47	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	189	48	membrane	membrane	NN
cord-104268-q1jx0n0l	189	49	-	-	HYPH
cord-104268-q1jx0n0l	189	50	spanning	span	VBG
cord-104268-q1jx0n0l	189	51	domain	domain	NN
cord-104268-q1jx0n0l	189	52	was	be	VBD
cord-104268-q1jx0n0l	189	53	made	make	VBN
cord-104268-q1jx0n0l	189	54	.	.	.
cord-104268-q1jx0n0l	190	1	As	as	IN
cord-104268-q1jx0n0l	190	2	was	be	VBD
cord-104268-q1jx0n0l	190	3	the	the	DT
cord-104268-q1jx0n0l	190	4	case	case	NN
cord-104268-q1jx0n0l	190	5	with	with	IN
cord-104268-q1jx0n0l	190	6	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	190	7	,	,	,
cord-104268-q1jx0n0l	190	8	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	190	9	was	be	VBD
cord-104268-q1jx0n0l	190	10	also	also	RB
cord-104268-q1jx0n0l	190	11	found	find	VBN
cord-104268-q1jx0n0l	190	12	to	to	TO
cord-104268-q1jx0n0l	190	13	be	be	VB
cord-104268-q1jx0n0l	190	14	localized	localize	VBN
cord-104268-q1jx0n0l	190	15	to	to	IN
cord-104268-q1jx0n0l	190	16	the	the	DT
cord-104268-q1jx0n0l	190	17	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	190	18	Hill	Hill	NNP
cord-104268-q1jx0n0l	190	19	et	et	NNP
cord-104268-q1jx0n0l	190	20	al	al	NNP
cord-104268-q1jx0n0l	190	21	.	.	NNP
cord-104268-q1jx0n0l	190	22	,	,	,
cord-104268-q1jx0n0l	190	23	1986	1986	CD
cord-104268-q1jx0n0l	190	24	)	)	-RRB-
cord-104268-q1jx0n0l	190	25	and	and	CC
cord-104268-q1jx0n0l	190	26	the	the	DT
cord-104268-q1jx0n0l	190	27	influenza	influenza	NN
cord-104268-q1jx0n0l	190	28	hemagglutinin	hemagglutinin	NNP
cord-104268-q1jx0n0l	190	29	virus	virus	NN
cord-104268-q1jx0n0l	190	30	epitope	epitope	NN
cord-104268-q1jx0n0l	190	31	-	-	HYPH
cord-104268-q1jx0n0l	190	32	tagged	tag	VBN
cord-104268-q1jx0n0l	190	33	MNN1	MNN1	NNP
cord-104268-q1jx0n0l	190	34	on	on	IN
cord-104268-q1jx0n0l	190	35	multicopy	multicopy	NNP
cord-104268-q1jx0n0l	190	36	plasmid	plasmid	NNP
cord-104268-q1jx0n0l	190	37	YEp352	YEp352	NNS
cord-104268-q1jx0n0l	190	38	(	(	-LRB-
cord-104268-q1jx0n0l	190	39	URA3	URA3	NNP
cord-104268-q1jx0n0l	190	40	;	;	:
cord-104268-q1jx0n0l	190	41	Hill	Hill	NNP
cord-104268-q1jx0n0l	190	42	et	et	NNP
cord-104268-q1jx0n0l	190	43	al	al	NNP
cord-104268-q1jx0n0l	190	44	.	.	NNP
cord-104268-q1jx0n0l	190	45	,	,	,
cord-104268-q1jx0n0l	190	46	1986	1986	CD
cord-104268-q1jx0n0l	190	47	)	)	-RRB-
cord-104268-q1jx0n0l	190	48	or	or	CC
cord-104268-q1jx0n0l	190	49	epitope	epitope	NN
cord-104268-q1jx0n0l	190	50	-	-	HYPH
cord-104268-q1jx0n0l	190	51	tagged	tagged	NNP
cord-104268-q1jx0n0l	190	52	KEX1	KEX1	NNP
cord-104268-q1jx0n0l	190	53	gene	gene	NN
cord-104268-q1jx0n0l	190	54	(	(	-LRB-
cord-104268-q1jx0n0l	190	55	see	see	VB
cord-104268-q1jx0n0l	190	56	Materials	material	NNS
cord-104268-q1jx0n0l	190	57	and	and	CC
cord-104268-q1jx0n0l	190	58	Methods	Methods	NNPS
cord-104268-q1jx0n0l	190	59	)	)	-RRB-
cord-104268-q1jx0n0l	190	60	under	under	IN
cord-104268-q1jx0n0l	190	61	an	an	DT
cord-104268-q1jx0n0l	190	62	ADH1	ADH1	NNP
cord-104268-q1jx0n0l	190	63	gene	gene	NN
cord-104268-q1jx0n0l	190	64	promoter	promoter	NN
cord-104268-q1jx0n0l	190	65	on	on	IN
cord-104268-q1jx0n0l	190	66	centromeric	centromeric	RB
cord-104268-q1jx0n0l	190	67	-	-	HYPH
cord-104268-q1jx0n0l	190	68	based	base	VBN
cord-104268-q1jx0n0l	190	69	plasmid	plasmid	NN
cord-104268-q1jx0n0l	190	70	pRS316	prs316	NN
cord-104268-q1jx0n0l	191	1	(	(	-LRB-
cord-104268-q1jx0n0l	191	2	URA3	URA3	NNP
cord-104268-q1jx0n0l	191	3	;	;	:
cord-104268-q1jx0n0l	191	4	Sikorski	Sikorski	NNP
cord-104268-q1jx0n0l	191	5	and	and	CC
cord-104268-q1jx0n0l	191	6	Hieter	Hieter	NNP
cord-104268-q1jx0n0l	191	7	,	,	,
cord-104268-q1jx0n0l	191	8	1989	1989	CD
cord-104268-q1jx0n0l	191	9	)	)	-RRB-
cord-104268-q1jx0n0l	191	10	were	be	VBD
cord-104268-q1jx0n0l	191	11	fixed	fix	VBN
cord-104268-q1jx0n0l	191	12	,	,	,
cord-104268-q1jx0n0l	191	13	spheroplasted	spheroplaste	VBN
cord-104268-q1jx0n0l	191	14	,	,	,
cord-104268-q1jx0n0l	191	15	attached	attach	VBN
cord-104268-q1jx0n0l	191	16	to	to	IN
cord-104268-q1jx0n0l	191	17	polylysine	polylysine	NN
cord-104268-q1jx0n0l	191	18	-	-	HYPH
cord-104268-q1jx0n0l	191	19	coated	coat	VBN
cord-104268-q1jx0n0l	191	20	glass	glass	NN
cord-104268-q1jx0n0l	191	21	slides	slide	NNS
cord-104268-q1jx0n0l	191	22	,	,	,
cord-104268-q1jx0n0l	191	23	and	and	CC
cord-104268-q1jx0n0l	191	24	then	then	RB
cord-104268-q1jx0n0l	191	25	incubated	incubate	VBN
cord-104268-q1jx0n0l	191	26	with	with	IN
cord-104268-q1jx0n0l	191	27	affinity	affinity	NN
cord-104268-q1jx0n0l	191	28	-	-	HYPH
cord-104268-q1jx0n0l	191	29	purified	purify	VBN
cord-104268-q1jx0n0l	191	30	anti	anti	NNP
cord-104268-q1jx0n0l	191	31	-	-	JJ
cord-104268-q1jx0n0l	191	32	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	191	33	and	and	CC
cord-104268-q1jx0n0l	191	34	12CA5	12ca5	CD
cord-104268-q1jx0n0l	191	35	mAb	mAb	NNS
cord-104268-q1jx0n0l	191	36	(	(	-LRB-
cord-104268-q1jx0n0l	191	37	Kolodziej	Kolodziej	NNP
cord-104268-q1jx0n0l	191	38	and	and	CC
cord-104268-q1jx0n0l	191	39	Young	Young	NNP
cord-104268-q1jx0n0l	191	40	,	,	,
cord-104268-q1jx0n0l	191	41	1991	1991	CD
cord-104268-q1jx0n0l	191	42	)	)	-RRB-
cord-104268-q1jx0n0l	191	43	.	.	.
cord-104268-q1jx0n0l	192	1	Fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	192	2	signals	signal	NNS
cord-104268-q1jx0n0l	192	3	were	be	VBD
cord-104268-q1jx0n0l	192	4	obtained	obtain	VBN
cord-104268-q1jx0n0l	192	5	by	by	IN
cord-104268-q1jx0n0l	192	6	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	192	7	incubation	incubation	NN
cord-104268-q1jx0n0l	192	8	of	of	IN
cord-104268-q1jx0n0l	192	9	treated	treat	VBN
cord-104268-q1jx0n0l	192	10	cells	cell	NNS
cord-104268-q1jx0n0l	192	11	with	with	IN
cord-104268-q1jx0n0l	192	12	Texas	Texas	NNP
cord-104268-q1jx0n0l	192	13	red	red	JJ
cord-104268-q1jx0n0l	192	14	-	-	HYPH
cord-104268-q1jx0n0l	192	15	conjugated	conjugated	JJ
cord-104268-q1jx0n0l	192	16	goat	goat	NN
cord-104268-q1jx0n0l	192	17	anti	anti	JJ
cord-104268-q1jx0n0l	192	18	-	-	JJ
cord-104268-q1jx0n0l	192	19	rabbit	rabbit	JJ
cord-104268-q1jx0n0l	192	20	IgG	igg	NN
cord-104268-q1jx0n0l	192	21	and	and	CC
cord-104268-q1jx0n0l	192	22	FITC	FITC	NNP
cord-104268-q1jx0n0l	192	23	-	-	HYPH
cord-104268-q1jx0n0l	192	24	conjugated	conjugate	VBN
cord-104268-q1jx0n0l	192	25	goat	goat	NN
cord-104268-q1jx0n0l	192	26	antimouse	antimouse	IN
cord-104268-q1jx0n0l	192	27	IgG	IgG	NNP
cord-104268-q1jx0n0l	192	28	which	which	WDT
cord-104268-q1jx0n0l	192	29	were	be	VBD
cord-104268-q1jx0n0l	192	30	used	use	VBN
cord-104268-q1jx0n0l	192	31	as	as	IN
cord-104268-q1jx0n0l	192	32	secondary	secondary	JJ
cord-104268-q1jx0n0l	192	33	antibodies	antibody	NNS
cord-104268-q1jx0n0l	192	34	.	.	.
cord-104268-q1jx0n0l	193	1	Arrows	arrow	NNS
cord-104268-q1jx0n0l	193	2	indicate	indicate	VBP
cord-104268-q1jx0n0l	193	3	colocalization	colocalization	NN
cord-104268-q1jx0n0l	193	4	of	of	IN
cord-104268-q1jx0n0l	193	5	punctiform	punctiform	NN
cord-104268-q1jx0n0l	193	6	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	193	7	.	.	.
cord-104268-q1jx0n0l	194	1	apparatus	apparatus	NN
cord-104268-q1jx0n0l	194	2	(	(	-LRB-
cord-104268-q1jx0n0l	194	3	Fig	Fig	NNP
cord-104268-q1jx0n0l	194	4	.	.	NNP
cord-104268-q1jx0n0l	194	5	7	7	CD
cord-104268-q1jx0n0l	194	6	)	)	-RRB-
cord-104268-q1jx0n0l	194	7	indicating	indicate	VBG
cord-104268-q1jx0n0l	194	8	that	that	IN
cord-104268-q1jx0n0l	194	9	the	the	DT
cord-104268-q1jx0n0l	194	10	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	194	11	TMD	TMD	NNP
cord-104268-q1jx0n0l	194	12	is	be	VBZ
cord-104268-q1jx0n0l	194	13	dispensable	dispensable	JJ
cord-104268-q1jx0n0l	194	14	for	for	IN
cord-104268-q1jx0n0l	194	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	194	16	retention	retention	NN
cord-104268-q1jx0n0l	194	17	.	.	.
cord-104268-q1jx0n0l	195	1	The	the	DT
cord-104268-q1jx0n0l	195	2	function	function	NN
cord-104268-q1jx0n0l	195	3	of	of	IN
cord-104268-q1jx0n0l	195	4	the	the	DT
cord-104268-q1jx0n0l	195	5	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	195	6	domain	domain	NN
cord-104268-q1jx0n0l	195	7	of	of	IN
cord-104268-q1jx0n0l	195	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	195	9	was	be	VBD
cord-104268-q1jx0n0l	195	10	examined	examine	VBN
cord-104268-q1jx0n0l	195	11	with	with	IN
cord-104268-q1jx0n0l	195	12	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	195	13	protein	protein	NN
cord-104268-q1jx0n0l	195	14	D	d	NN
cord-104268-q1jx0n0l	195	15	K	k	NN
cord-104268-q1jx0n0l	195	16	K	k	NN
cord-104268-q1jx0n0l	195	17	K	K	NNP
cord-104268-q1jx0n0l	195	18	which	which	WDT
cord-104268-q1jx0n0l	195	19	possesses	possess	VBZ
cord-104268-q1jx0n0l	195	20	the	the	DT
cord-104268-q1jx0n0l	195	21	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	195	22	NH2	NH2	NNP
cord-104268-q1jx0n0l	195	23	terminus	terminus	NN
cord-104268-q1jx0n0l	195	24	of	of	IN
cord-104268-q1jx0n0l	195	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	195	26	replaced	replace	VBN
cord-104268-q1jx0n0l	195	27	by	by	IN
cord-104268-q1jx0n0l	195	28	that	that	DT
cord-104268-q1jx0n0l	195	29	of	of	IN
cord-104268-q1jx0n0l	196	1	D	d	NN
cord-104268-q1jx0n0l	196	2	P	p	NN
cord-104268-q1jx0n0l	196	3	A	a	NN
cord-104268-q1jx0n0l	197	1	P	p	NN
cord-104268-q1jx0n0l	197	2	B.	b.	NN
cord-104268-q1jx0n0l	197	3	D	d	NN
cord-104268-q1jx0n0l	197	4	K	k	NN
cord-104268-q1jx0n0l	197	5	K	k	NN
cord-104268-q1jx0n0l	197	6	K	K	NNP
cord-104268-q1jx0n0l	197	7	could	could	MD
cord-104268-q1jx0n0l	197	8	not	not	RB
cord-104268-q1jx0n0l	197	9	be	be	VB
cord-104268-q1jx0n0l	197	10	visualized	visualize	VBN
cord-104268-q1jx0n0l	197	11	in	in	IN
cord-104268-q1jx0n0l	197	12	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	197	13	(	(	-LRB-
cord-104268-q1jx0n0l	197	14	data	datum	NNS
cord-104268-q1jx0n0l	197	15	not	not	RB
cord-104268-q1jx0n0l	197	16	shown	show	VBN
cord-104268-q1jx0n0l	197	17	)	)	-RRB-
cord-104268-q1jx0n0l	197	18	suggesting	suggest	VBG
cord-104268-q1jx0n0l	197	19	possible	possible	JJ
cord-104268-q1jx0n0l	197	20	degradation	degradation	NN
cord-104268-q1jx0n0l	197	21	of	of	IN
cord-104268-q1jx0n0l	197	22	this	this	DT
cord-104268-q1jx0n0l	197	23	protein	protein	NN
cord-104268-q1jx0n0l	197	24	in	in	IN
cord-104268-q1jx0n0l	197	25	the	the	DT
cord-104268-q1jx0n0l	197	26	yeast	yeast	NN
cord-104268-q1jx0n0l	197	27	vacuole	vacuole	NN
cord-104268-q1jx0n0l	197	28	as	as	IN
cord-104268-q1jx0n0l	197	29	found	find	VBN
cord-104268-q1jx0n0l	197	30	with	with	IN
cord-104268-q1jx0n0l	197	31	other	other	JJ
cord-104268-q1jx0n0l	197	32	mistargeted	mistargete	VBN
cord-104268-q1jx0n0l	197	33	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	197	34	proteins	protein	NNS
cord-104268-q1jx0n0l	197	35	(	(	-LRB-
cord-104268-q1jx0n0l	197	36	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	197	37	and	and	CC
cord-104268-q1jx0n0l	197	38	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	197	39	,	,	,
cord-104268-q1jx0n0l	197	40	1992	1992	CD
cord-104268-q1jx0n0l	197	41	;	;	.
cord-104268-q1jx0n0l	198	1	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	198	2	et	et	NNP
cord-104268-q1jx0n0l	198	3	al	al	NNP
cord-104268-q1jx0n0l	198	4	.	.	.
cord-104268-q1jx0n0l	199	1	,	,	,
cord-104268-q1jx0n0l	199	2	1992	1992	CD
cord-104268-q1jx0n0l	199	3	;	;	:
cord-104268-q1jx0n0l	199	4	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	199	5	et	et	NNP
cord-104268-q1jx0n0l	199	6	al	al	NNP
cord-104268-q1jx0n0l	199	7	.	.	.
cord-104268-q1jx0n0l	200	1	,	,	,
cord-104268-q1jx0n0l	200	2	1992	1992	CD
cord-104268-q1jx0n0l	200	3	;	;	:
cord-104268-q1jx0n0l	200	4	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	200	5	et	et	NNP
cord-104268-q1jx0n0l	200	6	al	al	NNP
cord-104268-q1jx0n0l	200	7	.	.	NNP
cord-104268-q1jx0n0l	200	8	,	,	,
cord-104268-q1jx0n0l	200	9	1993	1993	CD
cord-104268-q1jx0n0l	200	10	)	)	-RRB-
cord-104268-q1jx0n0l	200	11	.	.	.
cord-104268-q1jx0n0l	201	1	To	to	TO
cord-104268-q1jx0n0l	201	2	Figure	figure	NN
cord-104268-q1jx0n0l	201	3	6	6	CD
cord-104268-q1jx0n0l	201	4	.	.	.
cord-104268-q1jx0n0l	202	1	Schematic	schematic	JJ
cord-104268-q1jx0n0l	202	2	representation	representation	NN
cord-104268-q1jx0n0l	202	3	of	of	IN
cord-104268-q1jx0n0l	202	4	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	202	5	proteins	protein	NNS
cord-104268-q1jx0n0l	202	6	used	use	VBN
cord-104268-q1jx0n0l	202	7	in	in	IN
cord-104268-q1jx0n0l	202	8	the	the	DT
cord-104268-q1jx0n0l	202	9	analysis	analysis	NN
cord-104268-q1jx0n0l	202	10	of	of	IN
cord-104268-q1jx0n0l	202	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	202	12	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	202	13	targeting	targeting	NN
cord-104268-q1jx0n0l	202	14	.	.	.
cord-104268-q1jx0n0l	203	1	The	the	DT
cord-104268-q1jx0n0l	203	2	Kre2	Kre2	NNP
cord-104268-q1jx0n0l	203	3	protein	protein	NN
cord-104268-q1jx0n0l	203	4	is	be	VBZ
cord-104268-q1jx0n0l	203	5	composed	compose	VBN
cord-104268-q1jx0n0l	203	6	of	of	IN
cord-104268-q1jx0n0l	203	7	442	442	CD
cord-104268-q1jx0n0l	203	8	amino	amino	NN
cord-104268-q1jx0n0l	203	9	acid	acid	NN
cord-104268-q1jx0n0l	203	10	residues	residue	NNS
cord-104268-q1jx0n0l	203	11	.	.	.
cord-104268-q1jx0n0l	204	1	The	the	DT
cord-104268-q1jx0n0l	204	2	length	length	NN
cord-104268-q1jx0n0l	204	3	of	of	IN
cord-104268-q1jx0n0l	204	4	the	the	DT
cord-104268-q1jx0n0l	204	5	different	different	JJ
cord-104268-q1jx0n0l	204	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	204	7	domains	domain	NNS
cord-104268-q1jx0n0l	204	8	are	be	VBP
cord-104268-q1jx0n0l	204	9	as	as	IN
cord-104268-q1jx0n0l	204	10	follows	follow	VBZ
cord-104268-q1jx0n0l	204	11	:	:	:
cord-104268-q1jx0n0l	205	1	NH2	NH2	NNP
cord-104268-q1jx0n0l	205	2	terminus	terminus	NN
cord-104268-q1jx0n0l	205	3	,	,	,
cord-104268-q1jx0n0l	205	4	11	11	CD
cord-104268-q1jx0n0l	205	5	residues	residue	NNS
cord-104268-q1jx0n0l	205	6	;	;	:
cord-104268-q1jx0n0l	205	7	TMD	TMD	NNP
cord-104268-q1jx0n0l	205	8	,	,	,
cord-104268-q1jx0n0l	205	9	19	19	CD
cord-104268-q1jx0n0l	205	10	residues	residue	NNS
cord-104268-q1jx0n0l	205	11	;	;	:
cord-104268-q1jx0n0l	205	12	stem	stem	NN
cord-104268-q1jx0n0l	205	13	,	,	,
cord-104268-q1jx0n0l	205	14	~87	~87	JJ
cord-104268-q1jx0n0l	205	15	residues	residue	NNS
cord-104268-q1jx0n0l	205	16	and	and	CC
cord-104268-q1jx0n0l	205	17	the	the	DT
cord-104268-q1jx0n0l	205	18	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	205	19	domain	domain	NN
cord-104268-q1jx0n0l	205	20	is	be	VBZ
cord-104268-q1jx0n0l	205	21	~325	~325	VBN
cord-104268-q1jx0n0l	205	22	amino	amino	JJ
cord-104268-q1jx0n0l	205	23	acid	acid	NN
cord-104268-q1jx0n0l	205	24	residues	residue	NNS
cord-104268-q1jx0n0l	205	25	long	long	RB
cord-104268-q1jx0n0l	205	26	.	.	.
cord-104268-q1jx0n0l	206	1	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	206	2	B	B	NNP
cord-104268-q1jx0n0l	206	3	is	be	VBZ
cord-104268-q1jx0n0l	206	4	an	an	DT
cord-104268-q1jx0n0l	206	5	841-amino	841-amino	CD
cord-104268-q1jx0n0l	206	6	acid	acid	NN
cord-104268-q1jx0n0l	206	7	residue	residue	NN
cord-104268-q1jx0n0l	206	8	type	type	NN
cord-104268-q1jx0n0l	206	9	II	II	NNP
cord-104268-q1jx0n0l	206	10	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	206	11	membrane	membrane	NN
cord-104268-q1jx0n0l	206	12	protein	protein	NN
cord-104268-q1jx0n0l	206	13	with	with	IN
cord-104268-q1jx0n0l	206	14	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	206	15	NH2-terminal	nh2-terminal	NN
cord-104268-q1jx0n0l	206	16	and	and	CC
cord-104268-q1jx0n0l	206	17	membrane	membrane	NN
cord-104268-q1jx0n0l	206	18	-	-	HYPH
cord-104268-q1jx0n0l	206	19	spanning	span	VBG
cord-104268-q1jx0n0l	206	20	domains	domain	NNS
cord-104268-q1jx0n0l	206	21	of	of	IN
cord-104268-q1jx0n0l	206	22	29	29	CD
cord-104268-q1jx0n0l	206	23	and	and	CC
cord-104268-q1jx0n0l	206	24	16	16	CD
cord-104268-q1jx0n0l	206	25	amino	amino	NN
cord-104268-q1jx0n0l	206	26	acid	acid	NN
cord-104268-q1jx0n0l	206	27	residues	residue	NNS
cord-104268-q1jx0n0l	206	28	,	,	,
cord-104268-q1jx0n0l	206	29	respectively	respectively	RB
cord-104268-q1jx0n0l	206	30	,	,	,
cord-104268-q1jx0n0l	206	31	and	and	CC
cord-104268-q1jx0n0l	206	32	a	a	DT
cord-104268-q1jx0n0l	206	33	luminal	luminal	JJ
cord-104268-q1jx0n0l	206	34	domain	domain	NN
cord-104268-q1jx0n0l	206	35	consisting	consist	VBG
cord-104268-q1jx0n0l	206	36	of	of	IN
cord-104268-q1jx0n0l	206	37	796	796	CD
cord-104268-q1jx0n0l	206	38	amino	amino	NN
cord-104268-q1jx0n0l	206	39	acid	acid	NN
cord-104268-q1jx0n0l	206	40	residues	residue	NNS
cord-104268-q1jx0n0l	206	41	.	.	.
cord-104268-q1jx0n0l	207	1	The	the	DT
cord-104268-q1jx0n0l	207	2	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	207	3	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	207	4	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	207	5	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	207	6	is	be	VBZ
cord-104268-q1jx0n0l	207	7	a	a	DT
cord-104268-q1jx0n0l	207	8	566-amino	566-amino	CD
cord-104268-q1jx0n0l	207	9	acid	acid	NN
cord-104268-q1jx0n0l	207	10	residue	residue	NN
cord-104268-q1jx0n0l	207	11	type	type	NN
cord-104268-q1jx0n0l	207	12	II	II	NNP
cord-104268-q1jx0n0l	207	13	membrane	membrane	NN
cord-104268-q1jx0n0l	207	14	protein	protein	NN
cord-104268-q1jx0n0l	207	15	.	.	.
cord-104268-q1jx0n0l	208	1	The	the	DT
cord-104268-q1jx0n0l	208	2	length	length	NN
cord-104268-q1jx0n0l	208	3	of	of	IN
cord-104268-q1jx0n0l	208	4	the	the	DT
cord-104268-q1jx0n0l	208	5	different	different	JJ
cord-104268-q1jx0n0l	208	6	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	208	7	domains	domain	NNS
cord-104268-q1jx0n0l	208	8	are	be	VBP
cord-104268-q1jx0n0l	208	9	as	as	IN
cord-104268-q1jx0n0l	208	10	follows	follow	VBZ
cord-104268-q1jx0n0l	208	11	:	:	:
cord-104268-q1jx0n0l	209	1	NHz	NHz	NNP
cord-104268-q1jx0n0l	209	2	terminus	terminus	NN
cord-104268-q1jx0n0l	209	3	,	,	,
cord-104268-q1jx0n0l	209	4	33	33	CD
cord-104268-q1jx0n0l	209	5	residues	residue	NNS
cord-104268-q1jx0n0l	209	6	;	;	:
cord-104268-q1jx0n0l	209	7	TMD	TMD	NNP
cord-104268-q1jx0n0l	209	8	,	,	,
cord-104268-q1jx0n0l	209	9	26	26	CD
cord-104268-q1jx0n0l	209	10	residues	residue	NNS
cord-104268-q1jx0n0l	209	11	;	;	:
cord-104268-q1jx0n0l	209	12	and	and	CC
cord-104268-q1jx0n0l	209	13	the	the	DT
cord-104268-q1jx0n0l	209	14	luminal	luminal	JJ
cord-104268-q1jx0n0l	209	15	domain	domain	NN
cord-104268-q1jx0n0l	209	16	is	be	VBZ
cord-104268-q1jx0n0l	209	17	507	507	CD
cord-104268-q1jx0n0l	209	18	amino	amino	NN
cord-104268-q1jx0n0l	209	19	acid	acid	NN
cord-104268-q1jx0n0l	209	20	residues	residue	NNS
cord-104268-q1jx0n0l	209	21	long	long	RB
cord-104268-q1jx0n0l	209	22	.	.	.
cord-104268-q1jx0n0l	210	1	The	the	DT
cord-104268-q1jx0n0l	210	2	different	different	JJ
cord-104268-q1jx0n0l	210	3	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	210	4	constructions	construction	NNS
cord-104268-q1jx0n0l	210	5	were	be	VBD
cord-104268-q1jx0n0l	210	6	made	make	VBN
cord-104268-q1jx0n0l	210	7	by	by	IN
cord-104268-q1jx0n0l	210	8	replacing	replace	VBG
cord-104268-q1jx0n0l	210	9	or	or	CC
cord-104268-q1jx0n0l	210	10	fusing	fuse	VBG
cord-104268-q1jx0n0l	210	11	specific	specific	JJ
cord-104268-q1jx0n0l	210	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	210	13	domains	domain	NNS
cord-104268-q1jx0n0l	210	14	with	with	IN
cord-104268-q1jx0n0l	210	15	those	those	DT
cord-104268-q1jx0n0l	210	16	of	of	IN
cord-104268-q1jx0n0l	210	17	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	210	18	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	210	19	B	B	NNP
cord-104268-q1jx0n0l	210	20	and	and	CC
cord-104268-q1jx0n0l	210	21	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	210	22	.	.	.
cord-104268-q1jx0n0l	211	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	211	2	proteins	protein	NNS
cord-104268-q1jx0n0l	211	3	are	be	VBP
cord-104268-q1jx0n0l	211	4	represented	represent	VBN
cord-104268-q1jx0n0l	211	5	(	(	-LRB-
cord-104268-q1jx0n0l	211	6	not	not	RB
cord-104268-q1jx0n0l	211	7	to	to	TO
cord-104268-q1jx0n0l	211	8	scale	scale	VB
cord-104268-q1jx0n0l	211	9	)	)	-RRB-
cord-104268-q1jx0n0l	211	10	as	as	IN
cord-104268-q1jx0n0l	211	11	an	an	DT
cord-104268-q1jx0n0l	211	12	assembly	assembly	NN
cord-104268-q1jx0n0l	211	13	of	of	IN
cord-104268-q1jx0n0l	211	14	different	different	JJ
cord-104268-q1jx0n0l	211	15	domains	domain	NNS
cord-104268-q1jx0n0l	211	16	which	which	WDT
cord-104268-q1jx0n0l	211	17	are	be	VBP
cord-104268-q1jx0n0l	211	18	distinguished	distinguish	VBN
cord-104268-q1jx0n0l	211	19	by	by	IN
cord-104268-q1jx0n0l	211	20	a	a	DT
cord-104268-q1jx0n0l	211	21	letter	letter	NN
cord-104268-q1jx0n0l	211	22	specifying	specify	VBG
cord-104268-q1jx0n0l	211	23	their	-PRON-	PRP$
cord-104268-q1jx0n0l	211	24	origin	origin	NN
cord-104268-q1jx0n0l	211	25	.	.	.
cord-104268-q1jx0n0l	212	1	K	K	NNP
cord-104268-q1jx0n0l	212	2	,	,	,
cord-104268-q1jx0n0l	212	3	D	D	NNP
cord-104268-q1jx0n0l	212	4	,	,	,
cord-104268-q1jx0n0l	212	5	and	and	CC
cord-104268-q1jx0n0l	212	6	P	p	NN
cord-104268-q1jx0n0l	212	7	,	,	,
cord-104268-q1jx0n0l	212	8	respectively	respectively	RB
cord-104268-q1jx0n0l	212	9	,	,	,
cord-104268-q1jx0n0l	212	10	denote	denote	VB
cord-104268-q1jx0n0l	212	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	212	12	,	,	,
cord-104268-q1jx0n0l	212	13	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	212	14	B	B	NNP
cord-104268-q1jx0n0l	212	15	,	,	,
cord-104268-q1jx0n0l	212	16	and	and	CC
cord-104268-q1jx0n0l	212	17	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	212	18	.	.	.
cord-104268-q1jx0n0l	213	1	MR	MR	NNP
cord-104268-q1jx0n0l	213	2	represents	represent	VBZ
cord-104268-q1jx0n0l	213	3	the	the	DT
cord-104268-q1jx0n0l	213	4	two	two	CD
cord-104268-q1jx0n0l	213	5	remaining	remain	VBG
cord-104268-q1jx0n0l	213	6	amino	amino	NN
cord-104268-q1jx0n0l	213	7	acid	acid	NN
cord-104268-q1jx0n0l	213	8	residues	residue	NNS
cord-104268-q1jx0n0l	213	9	in	in	IN
cord-104268-q1jx0n0l	213	10	the	the	DT
cord-104268-q1jx0n0l	213	11	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	213	12	NH2	NH2	NNP
cord-104268-q1jx0n0l	213	13	terminus	terminus	NN
cord-104268-q1jx0n0l	213	14	of	of	IN
cord-104268-q1jx0n0l	213	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	213	16	after	after	IN
cord-104268-q1jx0n0l	213	17	truncation	truncation	NN
cord-104268-q1jx0n0l	213	18	.	.	.
cord-104268-q1jx0n0l	214	1	Putative	putative	JJ
cord-104268-q1jx0n0l	214	2	N	n	NN
cord-104268-q1jx0n0l	214	3	-	-	HYPH
cord-104268-q1jx0n0l	214	4	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	214	5	sites	site	NNS
cord-104268-q1jx0n0l	214	6	are	be	VBP
cord-104268-q1jx0n0l	214	7	indicated	indicate	VBN
cord-104268-q1jx0n0l	214	8	(	(	-LRB-
cord-104268-q1jx0n0l	214	9	Y	Y	NNP
cord-104268-q1jx0n0l	214	10	)	)	-RRB-
cord-104268-q1jx0n0l	214	11	.	.	.
cord-104268-q1jx0n0l	215	1	For	for	IN
cord-104268-q1jx0n0l	215	2	details	detail	NNS
cord-104268-q1jx0n0l	215	3	on	on	IN
cord-104268-q1jx0n0l	215	4	constructions	construction	NNS
cord-104268-q1jx0n0l	215	5	,	,	,
cord-104268-q1jx0n0l	215	6	see	see	VB
cord-104268-q1jx0n0l	215	7	Materials	material	NNS
cord-104268-q1jx0n0l	215	8	and	and	CC
cord-104268-q1jx0n0l	215	9	Methods	method	NNS
cord-104268-q1jx0n0l	215	10	.	.	.
cord-104268-q1jx0n0l	216	1	test	test	VB
cord-104268-q1jx0n0l	216	2	this	this	DT
cord-104268-q1jx0n0l	216	3	possibility	possibility	NN
cord-104268-q1jx0n0l	216	4	,	,	,
cord-104268-q1jx0n0l	216	5	D	d	NN
cord-104268-q1jx0n0l	216	6	K	k	NN
cord-104268-q1jx0n0l	216	7	K	k	NN
cord-104268-q1jx0n0l	216	8	K	K	NNP
cord-104268-q1jx0n0l	216	9	was	be	VBD
cord-104268-q1jx0n0l	216	10	expressed	express	VBN
cord-104268-q1jx0n0l	216	11	in	in	IN
cord-104268-q1jx0n0l	216	12	strain	strain	NN
cord-104268-q1jx0n0l	216	13	$	$	$
cord-104268-q1jx0n0l	216	14	86	86	CD
cord-104268-q1jx0n0l	216	15	which	which	WDT
cord-104268-q1jx0n0l	216	16	contains	contain	VBZ
cord-104268-q1jx0n0l	216	17	mutations	mutation	NNS
cord-104268-q1jx0n0l	216	18	in	in	IN
cord-104268-q1jx0n0l	216	19	the	the	DT
cord-104268-q1jx0n0l	216	20	major	major	JJ
cord-104268-q1jx0n0l	216	21	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	216	22	hydrolases	hydrolase	NNS
cord-104268-q1jx0n0l	216	23	and	and	CC
cord-104268-q1jx0n0l	216	24	was	be	VBD
cord-104268-q1jx0n0l	216	25	found	find	VBN
cord-104268-q1jx0n0l	216	26	to	to	TO
cord-104268-q1jx0n0l	216	27	be	be	VB
cord-104268-q1jx0n0l	216	28	exclusively	exclusively	RB
cord-104268-q1jx0n0l	216	29	mislocalized	mislocalize	VBN
cord-104268-q1jx0n0l	216	30	to	to	IN
cord-104268-q1jx0n0l	216	31	the	the	DT
cord-104268-q1jx0n0l	216	32	yeast	yeast	NN
cord-104268-q1jx0n0l	216	33	vacuole	vacuole	NN
cord-104268-q1jx0n0l	216	34	as	as	IN
cord-104268-q1jx0n0l	216	35	shown	show	VBN
cord-104268-q1jx0n0l	216	36	by	by	IN
cord-104268-q1jx0n0l	216	37	colocalization	colocalization	NN
cord-104268-q1jx0n0l	216	38	with	with	IN
cord-104268-q1jx0n0l	216	39	a	a	DT
cord-104268-q1jx0n0l	216	40	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	216	41	membrane	membrane	NN
cord-104268-q1jx0n0l	216	42	marker	marker	NN
cord-104268-q1jx0n0l	216	43	(	(	-LRB-
cord-104268-q1jx0n0l	216	44	Fig	Fig	NNP
cord-104268-q1jx0n0l	216	45	.	.	.
cord-104268-q1jx0n0l	216	46	8)	8)	NNP
cord-104268-q1jx0n0l	216	47	.	.	.
cord-104268-q1jx0n0l	217	1	In	in	IN
cord-104268-q1jx0n0l	217	2	contrast	contrast	NN
cord-104268-q1jx0n0l	217	3	,	,	,
cord-104268-q1jx0n0l	217	4	when	when	WRB
cord-104268-q1jx0n0l	217	5	wild	wild	JJ
cord-104268-q1jx0n0l	217	6	-	-	HYPH
cord-104268-q1jx0n0l	217	7	type	type	NN
cord-104268-q1jx0n0l	217	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	217	9	was	be	VBD
cord-104268-q1jx0n0l	217	10	expressed	express	VBN
cord-104268-q1jx0n0l	217	11	from	from	IN
cord-104268-q1jx0n0l	217	12	a	a	DT
cord-104268-q1jx0n0l	217	13	2p~-based	2p~-based	CD
cord-104268-q1jx0n0l	217	14	plasmid	plasmid	NN
cord-104268-q1jx0n0l	217	15	in	in	IN
cord-104268-q1jx0n0l	217	16	strain	strain	NN
cord-104268-q1jx0n0l	217	17	$	$	$
cord-104268-q1jx0n0l	217	18	86	86	CD
cord-104268-q1jx0n0l	217	19	,	,	,
cord-104268-q1jx0n0l	217	20	all	all	DT
cord-104268-q1jx0n0l	217	21	positive	positive	JJ
cord-104268-q1jx0n0l	217	22	cells	cell	NNS
cord-104268-q1jx0n0l	217	23	showed	show	VBD
cord-104268-q1jx0n0l	217	24	a	a	DT
cord-104268-q1jx0n0l	217	25	punctate	punctate	NN
cord-104268-q1jx0n0l	217	26	pattern	pattern	NN
cord-104268-q1jx0n0l	217	27	of	of	IN
cord-104268-q1jx0n0l	217	28	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	217	29	.	.	.
cord-104268-q1jx0n0l	218	1	However	however	RB
cord-104268-q1jx0n0l	218	2	,	,	,
cord-104268-q1jx0n0l	218	3	15	15	CD
cord-104268-q1jx0n0l	218	4	%	%	NN
cord-104268-q1jx0n0l	218	5	of	of	IN
cord-104268-q1jx0n0l	218	6	these	these	DT
cord-104268-q1jx0n0l	218	7	stained	stain	VBN
cord-104268-q1jx0n0l	218	8	cells	cell	NNS
cord-104268-q1jx0n0l	218	9	also	also	RB
cord-104268-q1jx0n0l	218	10	showed	show	VBD
cord-104268-q1jx0n0l	218	11	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	218	12	associated	associate	VBN
cord-104268-q1jx0n0l	218	13	with	with	IN
cord-104268-q1jx0n0l	218	14	the	the	DT
cord-104268-q1jx0n0l	218	15	vacuole	vacuole	NN
cord-104268-q1jx0n0l	218	16	(	(	-LRB-
cord-104268-q1jx0n0l	218	17	data	datum	NNS
cord-104268-q1jx0n0l	218	18	not	not	RB
cord-104268-q1jx0n0l	218	19	shown	show	VBN
cord-104268-q1jx0n0l	218	20	)	)	-RRB-
cord-104268-q1jx0n0l	218	21	.	.	.
cord-104268-q1jx0n0l	219	1	These	these	DT
cord-104268-q1jx0n0l	219	2	vacuolar	vacuolar	NN
cord-104268-q1jx0n0l	219	3	stained	stain	VBN
cord-104268-q1jx0n0l	219	4	cells	cell	NNS
cord-104268-q1jx0n0l	219	5	appeared	appear	VBD
cord-104268-q1jx0n0l	219	6	to	to	TO
cord-104268-q1jx0n0l	219	7	be	be	VB
cord-104268-q1jx0n0l	219	8	expressing	express	VBG
cord-104268-q1jx0n0l	219	9	high	high	JJ
cord-104268-q1jx0n0l	219	10	levels	level	NNS
cord-104268-q1jx0n0l	219	11	of	of	IN
cord-104268-q1jx0n0l	219	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	219	13	as	as	IN
cord-104268-q1jx0n0l	219	14	they	-PRON-	PRP
cord-104268-q1jx0n0l	219	15	displayed	display	VBD
cord-104268-q1jx0n0l	219	16	high	high	JJ
cord-104268-q1jx0n0l	219	17	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	219	18	levels	level	NNS
cord-104268-q1jx0n0l	219	19	.	.	.
cord-104268-q1jx0n0l	220	1	The	the	DT
cord-104268-q1jx0n0l	220	2	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	220	3	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	220	4	tail	tail	NN
cord-104268-q1jx0n0l	220	5	NH2	NH2	NNP
cord-104268-q1jx0n0l	220	6	terminus	terminus	NN
cord-104268-q1jx0n0l	220	7	was	be	VBD
cord-104268-q1jx0n0l	220	8	deleted	delete	VBN
cord-104268-q1jx0n0l	220	9	to	to	TO
cord-104268-q1jx0n0l	220	10	further	further	RB
cord-104268-q1jx0n0l	220	11	explore	explore	VB
cord-104268-q1jx0n0l	220	12	its	-PRON-	PRP$
cord-104268-q1jx0n0l	220	13	role	role	NN
cord-104268-q1jx0n0l	220	14	in	in	IN
cord-104268-q1jx0n0l	220	15	targeting	target	VBG
cord-104268-q1jx0n0l	220	16	.	.	.
cord-104268-q1jx0n0l	221	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	221	2	construct	construct	VBP
cord-104268-q1jx0n0l	221	3	MR	MR	NNP
cord-104268-q1jx0n0l	221	4	/	/	SYM
cord-104268-q1jx0n0l	221	5	KKK	KKK	NNP
cord-104268-q1jx0n0l	221	6	lacks	lack	VBZ
cord-104268-q1jx0n0l	221	7	the	the	DT
cord-104268-q1jx0n0l	221	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	221	9	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	221	10	tail	tail	NN
cord-104268-q1jx0n0l	221	11	,	,	,
cord-104268-q1jx0n0l	221	12	with	with	IN
cord-104268-q1jx0n0l	221	13	the	the	DT
cord-104268-q1jx0n0l	221	14	exception	exception	NN
cord-104268-q1jx0n0l	221	15	of	of	IN
cord-104268-q1jx0n0l	221	16	the	the	DT
cord-104268-q1jx0n0l	221	17	initiating	initiate	VBG
cord-104268-q1jx0n0l	221	18	methionine	methionine	NN
cord-104268-q1jx0n0l	221	19	codon	codon	NN
cord-104268-q1jx0n0l	221	20	and	and	CC
cord-104268-q1jx0n0l	221	21	the	the	DT
cord-104268-q1jx0n0l	221	22	Arg	arg	NN
cord-104268-q1jx0n0l	221	23	residue	residue	NN
cord-104268-q1jx0n0l	221	24	lying	lie	VBG
cord-104268-q1jx0n0l	221	25	directly	directly	RB
cord-104268-q1jx0n0l	221	26	adjacent	adjacent	JJ
cord-104268-q1jx0n0l	221	27	to	to	IN
cord-104268-q1jx0n0l	221	28	the	the	DT
cord-104268-q1jx0n0l	221	29	TMD	TMD	NNP
cord-104268-q1jx0n0l	221	30	.	.	.
cord-104268-q1jx0n0l	222	1	MR	MR	NNP
cord-104268-q1jx0n0l	222	2	/	/	SYM
cord-104268-q1jx0n0l	222	3	KKK	KKK	NNP
cord-104268-q1jx0n0l	222	4	was	be	VBD
cord-104268-q1jx0n0l	222	5	Figure	Figure	NNP
cord-104268-q1jx0n0l	222	6	7	7	CD
cord-104268-q1jx0n0l	222	7	.	.	.
cord-104268-q1jx0n0l	223	1	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	223	2	localization	localization	NN
cord-104268-q1jx0n0l	223	3	of	of	IN
cord-104268-q1jx0n0l	223	4	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	223	5	proteins	protein	NNS
cord-104268-q1jx0n0l	223	6	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	223	7	,	,	,
cord-104268-q1jx0n0l	223	8	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	223	9	,	,	,
cord-104268-q1jx0n0l	223	10	and	and	CC
cord-104268-q1jx0n0l	223	11	KD	KD	NNP
cord-104268-q1jx0n0l	223	12	-	-	HYPH
cord-104268-q1jx0n0l	223	13	K	K	NNP
cord-104268-q1jx0n0l	223	14	by	by	IN
cord-104268-q1jx0n0l	223	15	indirect	indirect	JJ
cord-104268-q1jx0n0l	223	16	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	223	17	.	.	.
cord-104268-q1jx0n0l	224	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	224	2	ceils	ceil	NNS
cord-104268-q1jx0n0l	224	3	(	(	-LRB-
cord-104268-q1jx0n0l	224	4	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	224	5	)	)	-RRB-
cord-104268-q1jx0n0l	224	6	carrying	carry	VBG
cord-104268-q1jx0n0l	224	7	a	a	DT
cord-104268-q1jx0n0l	224	8	KRE2	kre2	JJ
cord-104268-q1jx0n0l	224	9	disruption	disruption	NN
cord-104268-q1jx0n0l	224	10	(	(	-LRB-
cord-104268-q1jx0n0l	224	11	kre2::TRP1	kre2::TRP1	NNP
cord-104268-q1jx0n0l	224	12	)	)	-RRB-
cord-104268-q1jx0n0l	224	13	and	and	CC
cord-104268-q1jx0n0l	224	14	containing	contain	VBG
cord-104268-q1jx0n0l	224	15	the	the	DT
cord-104268-q1jx0n0l	224	16	different	different	JJ
cord-104268-q1jx0n0l	224	17	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	224	18	constructions	construction	NNS
cord-104268-q1jx0n0l	224	19	on	on	IN
cord-104268-q1jx0n0l	224	20	YEp352	YEp352	NNS
cord-104268-q1jx0n0l	224	21	were	be	VBD
cord-104268-q1jx0n0l	224	22	fixed	fix	VBN
cord-104268-q1jx0n0l	224	23	,	,	,
cord-104268-q1jx0n0l	224	24	spheroplasted	spheroplaste	VBN
cord-104268-q1jx0n0l	224	25	,	,	,
cord-104268-q1jx0n0l	224	26	attached	attach	VBN
cord-104268-q1jx0n0l	224	27	to	to	IN
cord-104268-q1jx0n0l	224	28	polylysine	polylysine	NN
cord-104268-q1jx0n0l	224	29	-	-	HYPH
cord-104268-q1jx0n0l	224	30	coated	coat	VBN
cord-104268-q1jx0n0l	224	31	glass	glass	NN
cord-104268-q1jx0n0l	224	32	slides	slide	NNS
cord-104268-q1jx0n0l	224	33	,	,	,
cord-104268-q1jx0n0l	224	34	and	and	CC
cord-104268-q1jx0n0l	224	35	incubated	incubate	VBN
cord-104268-q1jx0n0l	224	36	with	with	IN
cord-104268-q1jx0n0l	224	37	anti	anti	JJ
cord-104268-q1jx0n0l	224	38	-	-	JJ
cord-104268-q1jx0n0l	224	39	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	224	40	antibodies	antibody	NNS
cord-104268-q1jx0n0l	224	41	followed	follow	VBN
cord-104268-q1jx0n0l	224	42	by	by	IN
cord-104268-q1jx0n0l	224	43	a	a	DT
cord-104268-q1jx0n0l	224	44	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	224	45	incubation	incubation	NN
cord-104268-q1jx0n0l	224	46	with	with	IN
cord-104268-q1jx0n0l	224	47	a	a	DT
cord-104268-q1jx0n0l	224	48	Texas	Texas	NNP
cord-104268-q1jx0n0l	224	49	red	red	JJ
cord-104268-q1jx0n0l	224	50	-	-	HYPH
cord-104268-q1jx0n0l	224	51	conjugated	conjugated	JJ
cord-104268-q1jx0n0l	224	52	secondary	secondary	JJ
cord-104268-q1jx0n0l	224	53	Ab	Ab	NNS
cord-104268-q1jx0n0l	224	54	and	and	CC
cord-104268-q1jx0n0l	224	55	DAPI	DAPI	NNP
cord-104268-q1jx0n0l	224	56	.	.	.
cord-104268-q1jx0n0l	225	1	found	find	VBN
cord-104268-q1jx0n0l	225	2	to	to	TO
cord-104268-q1jx0n0l	225	3	be	be	VB
cord-104268-q1jx0n0l	225	4	mislocalized	mislocalize	VBN
cord-104268-q1jx0n0l	225	5	to	to	IN
cord-104268-q1jx0n0l	225	6	the	the	DT
cord-104268-q1jx0n0l	225	7	vacuole	vacuole	NN
cord-104268-q1jx0n0l	226	1	(	(	-LRB-
cord-104268-q1jx0n0l	226	2	Fig	Fig	NNP
cord-104268-q1jx0n0l	226	3	.	.	.
cord-104268-q1jx0n0l	226	4	8)	8)	NNP
cord-104268-q1jx0n0l	226	5	,	,	,
cord-104268-q1jx0n0l	226	6	again	again	RB
cord-104268-q1jx0n0l	226	7	demonstrating	demonstrate	VBG
cord-104268-q1jx0n0l	226	8	that	that	IN
cord-104268-q1jx0n0l	226	9	the	the	DT
cord-104268-q1jx0n0l	226	10	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	226	11	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	226	12	domain	domain	NN
cord-104268-q1jx0n0l	226	13	of	of	IN
cord-104268-q1jx0n0l	226	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	226	15	is	be	VBZ
cord-104268-q1jx0n0l	226	16	required	require	VBN
cord-104268-q1jx0n0l	226	17	for	for	IN
cord-104268-q1jx0n0l	226	18	correct	correct	JJ
cord-104268-q1jx0n0l	226	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	226	20	localization	localization	NN
cord-104268-q1jx0n0l	226	21	.	.	.
cord-104268-q1jx0n0l	227	1	Some	some	DT
cord-104268-q1jx0n0l	227	2	ER	ER	NNP
cord-104268-q1jx0n0l	227	3	retention	retention	NN
cord-104268-q1jx0n0l	227	4	of	of	IN
cord-104268-q1jx0n0l	227	5	this	this	DT
cord-104268-q1jx0n0l	227	6	MR	MR	NNP
cord-104268-q1jx0n0l	227	7	/	/	SYM
cord-104268-q1jx0n0l	227	8	KKK	KKK	NNP
cord-104268-q1jx0n0l	227	9	protein	protein	NN
cord-104268-q1jx0n0l	227	10	was	be	VBD
cord-104268-q1jx0n0l	227	11	seen	see	VBN
cord-104268-q1jx0n0l	227	12	in	in	IN
cord-104268-q1jx0n0l	227	13	~10	~10	CD
cord-104268-q1jx0n0l	227	14	%	%	NN
cord-104268-q1jx0n0l	227	15	of	of	IN
cord-104268-q1jx0n0l	227	16	positive	positive	JJ
cord-104268-q1jx0n0l	227	17	cells	cell	NNS
cord-104268-q1jx0n0l	227	18	,	,	,
cord-104268-q1jx0n0l	227	19	indicative	indicative	JJ
cord-104268-q1jx0n0l	227	20	of	of	IN
cord-104268-q1jx0n0l	227	21	an	an	DT
cord-104268-q1jx0n0l	227	22	increased	increase	VBN
cord-104268-q1jx0n0l	227	23	transit	transit	NN
cord-104268-q1jx0n0l	227	24	time	time	NN
cord-104268-q1jx0n0l	227	25	through	through	IN
cord-104268-q1jx0n0l	227	26	this	this	DT
cord-104268-q1jx0n0l	227	27	organelle	organelle	NN
cord-104268-q1jx0n0l	227	28	.	.	.
cord-104268-q1jx0n0l	228	1	The	the	DT
cord-104268-q1jx0n0l	228	2	role	role	NN
cord-104268-q1jx0n0l	228	3	of	of	IN
cord-104268-q1jx0n0l	228	4	the	the	DT
cord-104268-q1jx0n0l	228	5	luminal	luminal	JJ
cord-104268-q1jx0n0l	228	6	amino	amino	NN
cord-104268-q1jx0n0l	228	7	acid	acid	NN
cord-104268-q1jx0n0l	228	8	residues	residue	NNS
cord-104268-q1jx0n0l	228	9	flanking	flank	VBG
cord-104268-q1jx0n0l	228	10	the	the	DT
cord-104268-q1jx0n0l	228	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	228	12	TMD	TMD	NNP
cord-104268-q1jx0n0l	228	13	was	be	VBD
cord-104268-q1jx0n0l	228	14	examined	examine	VBN
cord-104268-q1jx0n0l	228	15	with	with	IN
cord-104268-q1jx0n0l	228	16	fusion	fusion	NN
cord-104268-q1jx0n0l	228	17	protein	protein	NN
cord-104268-q1jx0n0l	228	18	KD	KD	NNP
cord-104268-q1jx0n0l	228	19	-	-	HYPH
cord-104268-q1jx0n0l	228	20	K.	K.	NNP
cord-104268-q1jx0n0l	229	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	229	2	comprises	comprise	VBZ
cord-104268-q1jx0n0l	229	3	the	the	DT
cord-104268-q1jx0n0l	229	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	229	5	NH2	NH2	NNP
cord-104268-q1jx0n0l	229	6	terminus	terminus	NN
cord-104268-q1jx0n0l	229	7	,	,	,
cord-104268-q1jx0n0l	229	8	the	the	DT
cord-104268-q1jx0n0l	229	9	membrane	membrane	NN
cord-104268-q1jx0n0l	229	10	-	-	HYPH
cord-104268-q1jx0n0l	229	11	spanning	span	VBG
cord-104268-q1jx0n0l	229	12	domain	domain	NN
cord-104268-q1jx0n0l	229	13	of	of	IN
cord-104268-q1jx0n0l	229	14	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	229	15	B	B	NNP
cord-104268-q1jx0n0l	229	16	,	,	,
cord-104268-q1jx0n0l	229	17	the	the	DT
cord-104268-q1jx0n0l	229	18	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	229	19	stem	stem	NN
cord-104268-q1jx0n0l	229	20	region	region	NN
cord-104268-q1jx0n0l	229	21	lacking	lack	VBG
cord-104268-q1jx0n0l	229	22	the	the	DT
cord-104268-q1jx0n0l	229	23	first	first	JJ
cord-104268-q1jx0n0l	229	24	36	36	CD
cord-104268-q1jx0n0l	229	25	amino	amino	NN
cord-104268-q1jx0n0l	229	26	acid	acid	NN
cord-104268-q1jx0n0l	229	27	residues	residue	NNS
cord-104268-q1jx0n0l	229	28	following	follow	VBG
cord-104268-q1jx0n0l	229	29	the	the	DT
cord-104268-q1jx0n0l	229	30	TMD	TMD	NNP
cord-104268-q1jx0n0l	229	31	,	,	,
cord-104268-q1jx0n0l	229	32	and	and	CC
cord-104268-q1jx0n0l	229	33	the	the	DT
cord-104268-q1jx0n0l	229	34	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	229	35	domain	domain	NN
cord-104268-q1jx0n0l	229	36	of	of	IN
cord-104268-q1jx0n0l	229	37	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	229	38	.	.	.
cord-104268-q1jx0n0l	230	1	KD	KD	NNP
cord-104268-q1jx0n0l	230	2	-	-	HYPH
cord-104268-q1jx0n0l	230	3	K	K	NNP
cord-104268-q1jx0n0l	230	4	was	be	VBD
cord-104268-q1jx0n0l	230	5	found	find	VBN
cord-104268-q1jx0n0l	230	6	to	to	TO
cord-104268-q1jx0n0l	230	7	be	be	VB
cord-104268-q1jx0n0l	230	8	localized	localize	VBN
cord-104268-q1jx0n0l	230	9	in	in	IN
cord-104268-q1jx0n0l	230	10	the	the	DT
cord-104268-q1jx0n0l	230	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	230	12	complex	complex	NN
cord-104268-q1jx0n0l	230	13	(	(	-LRB-
cord-104268-q1jx0n0l	230	14	Fig	Fig	NNP
cord-104268-q1jx0n0l	230	15	.	.	NNP
cord-104268-q1jx0n0l	230	16	7	7	CD
cord-104268-q1jx0n0l	230	17	)	)	-RRB-
cord-104268-q1jx0n0l	230	18	suggesting	suggest	VBG
cord-104268-q1jx0n0l	230	19	that	that	IN
cord-104268-q1jx0n0l	230	20	neither	neither	CC
cord-104268-q1jx0n0l	230	21	the	the	DT
cord-104268-q1jx0n0l	230	22	deleted	delete	VBN
cord-104268-q1jx0n0l	230	23	stem	stem	NN
cord-104268-q1jx0n0l	230	24	region	region	NN
cord-104268-q1jx0n0l	230	25	nor	nor	CC
cord-104268-q1jx0n0l	230	26	the	the	DT
cord-104268-q1jx0n0l	230	27	TMD	TMD	NNP
cord-104268-q1jx0n0l	230	28	is	be	VBZ
cord-104268-q1jx0n0l	230	29	necessary	necessary	JJ
cord-104268-q1jx0n0l	230	30	for	for	IN
cord-104268-q1jx0n0l	230	31	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	230	32	retention	retention	NN
cord-104268-q1jx0n0l	230	33	in	in	IN
cord-104268-q1jx0n0l	230	34	the	the	DT
cord-104268-q1jx0n0l	230	35	context	context	NN
cord-104268-q1jx0n0l	230	36	of	of	IN
cord-104268-q1jx0n0l	230	37	a	a	DT
cord-104268-q1jx0n0l	230	38	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	230	39	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	230	40	domain	domain	NN
cord-104268-q1jx0n0l	230	41	.	.	.
cord-104268-q1jx0n0l	231	1	In	in	IN
cord-104268-q1jx0n0l	231	2	parallel	parallel	NN
cord-104268-q1jx0n0l	231	3	with	with	IN
cord-104268-q1jx0n0l	231	4	the	the	DT
cord-104268-q1jx0n0l	231	5	localization	localization	NN
cord-104268-q1jx0n0l	231	6	studies	study	NNS
cord-104268-q1jx0n0l	231	7	,	,	,
cord-104268-q1jx0n0l	231	8	all	all	DT
cord-104268-q1jx0n0l	231	9	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	231	10	proteins	protein	NNS
cord-104268-q1jx0n0l	231	11	were	be	VBD
cord-104268-q1jx0n0l	231	12	assessed	assess	VBN
cord-104268-q1jx0n0l	231	13	functionally	functionally	RB
cord-104268-q1jx0n0l	231	14	.	.	.
cord-104268-q1jx0n0l	232	1	They	-PRON-	PRP
cord-104268-q1jx0n0l	232	2	were	be	VBD
cord-104268-q1jx0n0l	232	3	verified	verify	VBN
cord-104268-q1jx0n0l	232	4	to	to	TO
cord-104268-q1jx0n0l	232	5	be	be	VB
cord-104268-q1jx0n0l	232	6	membrane	membrane	NN
cord-104268-q1jx0n0l	232	7	associated	associate	VBN
cord-104268-q1jx0n0l	232	8	and	and	CC
cord-104268-q1jx0n0l	232	9	properly	properly	RB
cord-104268-q1jx0n0l	232	10	glycosylated	glycosylate	VBN
cord-104268-q1jx0n0l	233	1	(	(	-LRB-
cord-104268-q1jx0n0l	233	2	data	datum	NNS
cord-104268-q1jx0n0l	233	3	not	not	RB
cord-104268-q1jx0n0l	233	4	shown	show	VBN
cord-104268-q1jx0n0l	233	5	)	)	-RRB-
cord-104268-q1jx0n0l	233	6	,	,	,
cord-104268-q1jx0n0l	233	7	showing	show	VBG
cord-104268-q1jx0n0l	233	8	that	that	IN
cord-104268-q1jx0n0l	233	9	they	-PRON-	PRP
cord-104268-q1jx0n0l	233	10	possess	possess	VBP
cord-104268-q1jx0n0l	233	11	a	a	DT
cord-104268-q1jx0n0l	233	12	type	type	NN
cord-104268-q1jx0n0l	233	13	II	II	NNP
cord-104268-q1jx0n0l	233	14	membrane	membrane	NN
cord-104268-q1jx0n0l	233	15	orientation	orientation	NN
cord-104268-q1jx0n0l	233	16	(	(	-LRB-
cord-104268-q1jx0n0l	233	17	see	see	VB
cord-104268-q1jx0n0l	233	18	Fig	Fig	NNP
cord-104268-q1jx0n0l	233	19	.	.	.
cord-104268-q1jx0n0l	233	20	6	6	CD
cord-104268-q1jx0n0l	233	21	)	)	-RRB-
cord-104268-q1jx0n0l	233	22	.	.	.
cord-104268-q1jx0n0l	234	1	In	in	IN
cord-104268-q1jx0n0l	234	2	addition	addition	NN
cord-104268-q1jx0n0l	234	3	,	,	,
cord-104268-q1jx0n0l	234	4	their	-PRON-	PRP$
cord-104268-q1jx0n0l	234	5	in	in	IN
cord-104268-q1jx0n0l	234	6	vitro	vitro	FW
cord-104268-q1jx0n0l	234	7	and	and	CC
cord-104268-q1jx0n0l	234	8	in	in	IN
cord-104268-q1jx0n0l	234	9	vivo	vivo	NN
cord-104268-q1jx0n0l	234	10	enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	234	11	activities	activity	NNS
cord-104268-q1jx0n0l	234	12	were	be	VBD
cord-104268-q1jx0n0l	234	13	assayed	assay	VBN
cord-104268-q1jx0n0l	234	14	since	since	IN
cord-104268-q1jx0n0l	234	15	low	low	JJ
cord-104268-q1jx0n0l	234	16	levels	level	NNS
cord-104268-q1jx0n0l	234	17	of	of	IN
cord-104268-q1jx0n0l	234	18	mannosyltransferase	mannosyltransferase	JJ
cord-104268-q1jx0n0l	234	19	activity	activity	NN
cord-104268-q1jx0n0l	234	20	would	would	MD
cord-104268-q1jx0n0l	234	21	imply	imply	VB
cord-104268-q1jx0n0l	234	22	abnormal	abnormal	JJ
cord-104268-q1jx0n0l	234	23	secondary	secondary	JJ
cord-104268-q1jx0n0l	234	24	structure	structure	NN
cord-104268-q1jx0n0l	234	25	of	of	IN
cord-104268-q1jx0n0l	234	26	a	a	DT
cord-104268-q1jx0n0l	234	27	particular	particular	JJ
cord-104268-q1jx0n0l	234	28	chimera	chimera	NN
cord-104268-q1jx0n0l	234	29	and	and	CC
cord-104268-q1jx0n0l	234	30	possible	possible	JJ
cord-104268-q1jx0n0l	234	31	mislocaliza	mislocaliza	NN
cord-104268-q1jx0n0l	234	32	-	-	HYPH
cord-104268-q1jx0n0l	234	33	tion	tion	NN
cord-104268-q1jx0n0l	234	34	in	in	IN
cord-104268-q1jx0n0l	234	35	the	the	DT
cord-104268-q1jx0n0l	234	36	secretory	secretory	JJ
cord-104268-q1jx0n0l	234	37	pathway	pathway	NN
cord-104268-q1jx0n0l	234	38	.	.	.
cord-104268-q1jx0n0l	235	1	The	the	DT
cord-104268-q1jx0n0l	235	2	in	in	IN
cord-104268-q1jx0n0l	235	3	vitro	vitro	FW
cord-104268-q1jx0n0l	235	4	cd,2-mannosyltransferase	cd,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	235	5	activity	activity	NN
cord-104268-q1jx0n0l	235	6	was	be	VBD
cord-104268-q1jx0n0l	235	7	assayed	assay	VBN
cord-104268-q1jx0n0l	235	8	in	in	IN
cord-104268-q1jx0n0l	235	9	cell	cell	NN
cord-104268-q1jx0n0l	235	10	-	-	HYPH
cord-104268-q1jx0n0l	235	11	membrane	membrane	NN
cord-104268-q1jx0n0l	235	12	fractions	fraction	NNS
cord-104268-q1jx0n0l	235	13	.	.	.
cord-104268-q1jx0n0l	236	1	The	the	DT
cord-104268-q1jx0n0l	236	2	specific	specific	JJ
cord-104268-q1jx0n0l	236	3	activity	activity	NN
cord-104268-q1jx0n0l	236	4	of	of	IN
cord-104268-q1jx0n0l	236	5	each	each	DT
cord-104268-q1jx0n0l	236	6	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	236	7	protein	protein	NN
cord-104268-q1jx0n0l	236	8	is	be	VBZ
cord-104268-q1jx0n0l	236	9	displayed	display	VBN
cord-104268-q1jx0n0l	236	10	in	in	IN
cord-104268-q1jx0n0l	236	11	Fig	Fig	NNP
cord-104268-q1jx0n0l	236	12	.	.	.
cord-104268-q1jx0n0l	237	1	9	9	LS
cord-104268-q1jx0n0l	237	2	,	,	,
cord-104268-q1jx0n0l	237	3	where	where	WRB
cord-104268-q1jx0n0l	237	4	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	237	5	expressed	express	VBD
cord-104268-q1jx0n0l	237	6	in	in	IN
cord-104268-q1jx0n0l	237	7	a	a	DT
cord-104268-q1jx0n0l	237	8	kre2A	kre2a	CD
cord-104268-q1jx0n0l	237	9	strain	strain	NN
cord-104268-q1jx0n0l	237	10	was	be	VBD
cord-104268-q1jx0n0l	237	11	arbitrarily	arbitrarily	RB
cord-104268-q1jx0n0l	237	12	determined	determine	VBN
cord-104268-q1jx0n0l	237	13	to	to	TO
cord-104268-q1jx0n0l	237	14	be	be	VB
cord-104268-q1jx0n0l	237	15	100	100	CD
cord-104268-q1jx0n0l	237	16	%	%	NN
cord-104268-q1jx0n0l	237	17	,	,	,
cord-104268-q1jx0n0l	237	18	a	a	DT
cord-104268-q1jx0n0l	237	19	value	value	NN
cord-104268-q1jx0n0l	237	20	just	just	RB
cord-104268-q1jx0n0l	237	21	slightly	slightly	RB
cord-104268-q1jx0n0l	237	22	lower	low	JJR
cord-104268-q1jx0n0l	237	23	than	than	IN
cord-104268-q1jx0n0l	237	24	that	that	DT
cord-104268-q1jx0n0l	237	25	of	of	IN
cord-104268-q1jx0n0l	237	26	wild	wild	JJ
cord-104268-q1jx0n0l	237	27	-	-	HYPH
cord-104268-q1jx0n0l	237	28	type	type	NN
cord-104268-q1jx0n0l	237	29	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	237	30	activity	activity	NN
cord-104268-q1jx0n0l	237	31	from	from	IN
cord-104268-q1jx0n0l	237	32	a	a	DT
cord-104268-q1jx0n0l	237	33	genomic	genomic	JJ
cord-104268-q1jx0n0l	237	34	copy	copy	NN
cord-104268-q1jx0n0l	237	35	of	of	IN
cord-104268-q1jx0n0l	237	36	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	237	37	.	.	.
cord-104268-q1jx0n0l	238	1	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	238	2	,	,	,
cord-104268-q1jx0n0l	238	3	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	238	4	,	,	,
cord-104268-q1jx0n0l	238	5	DKKK	DKKK	NNP
cord-104268-q1jx0n0l	238	6	,	,	,
cord-104268-q1jx0n0l	238	7	as	as	RB
cord-104268-q1jx0n0l	238	8	well	well	RB
cord-104268-q1jx0n0l	238	9	as	as	IN
cord-104268-q1jx0n0l	238	10	MR	MR	NNP
cord-104268-q1jx0n0l	238	11	/	/	SYM
cord-104268-q1jx0n0l	238	12	KKK	KKK	NNP
cord-104268-q1jx0n0l	238	13	appear	appear	VBP
cord-104268-q1jx0n0l	238	14	to	to	TO
cord-104268-q1jx0n0l	238	15	be	be	VB
cord-104268-q1jx0n0l	238	16	fully	fully	RB
cord-104268-q1jx0n0l	238	17	active	active	JJ
cord-104268-q1jx0n0l	238	18	.	.	.
cord-104268-q1jx0n0l	239	1	KD	KD	NNP
cord-104268-q1jx0n0l	239	2	-	-	HYPH
cord-104268-q1jx0n0l	239	3	K	K	NNP
cord-104268-q1jx0n0l	239	4	displays	display	VBZ
cord-104268-q1jx0n0l	239	5	intermediate	intermediate	JJ
cord-104268-q1jx0n0l	239	6	levels	level	NNS
cord-104268-q1jx0n0l	239	7	of	of	IN
cord-104268-q1jx0n0l	239	8	mannosyltransferase	mannosyltransferase	JJ
cord-104268-q1jx0n0l	239	9	activity	activity	NN
cord-104268-q1jx0n0l	239	10	suggesting	suggest	VBG
cord-104268-q1jx0n0l	239	11	that	that	IN
cord-104268-q1jx0n0l	239	12	the	the	DT
cord-104268-q1jx0n0l	239	13	stem	stem	NN
cord-104268-q1jx0n0l	239	14	region	region	NN
cord-104268-q1jx0n0l	239	15	from	from	IN
cord-104268-q1jx0n0l	239	16	position	position	NN
cord-104268-q1jx0n0l	239	17	31	31	CD
cord-104268-q1jx0n0l	239	18	to	to	IN
cord-104268-q1jx0n0l	239	19	66	66	CD
cord-104268-q1jx0n0l	239	20	is	be	VBZ
cord-104268-q1jx0n0l	239	21	necessary	necessary	JJ
cord-104268-q1jx0n0l	239	22	for	for	IN
cord-104268-q1jx0n0l	239	23	folding	fold	VBG
cord-104268-q1jx0n0l	239	24	or	or	CC
cord-104268-q1jx0n0l	239	25	proper	proper	JJ
cord-104268-q1jx0n0l	239	26	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	239	27	activity	activity	NN
cord-104268-q1jx0n0l	239	28	.	.	.
cord-104268-q1jx0n0l	240	1	The	the	DT
cord-104268-q1jx0n0l	240	2	capacity	capacity	NN
cord-104268-q1jx0n0l	240	3	of	of	IN
cord-104268-q1jx0n0l	240	4	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	240	5	proteins	protein	NNS
cord-104268-q1jx0n0l	240	6	to	to	TO
cord-104268-q1jx0n0l	240	7	function	function	VB
cord-104268-q1jx0n0l	240	8	in	in	IN
cord-104268-q1jx0n0l	240	9	vivo	vivo	NN
cord-104268-q1jx0n0l	240	10	in	in	IN
cord-104268-q1jx0n0l	240	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	240	12	-	-	HYPH
cord-104268-q1jx0n0l	240	13	localized	localize	VBN
cord-104268-q1jx0n0l	240	14	mannosylation	mannosylation	NN
cord-104268-q1jx0n0l	240	15	was	be	VBD
cord-104268-q1jx0n0l	240	16	assessed	assess	VBN
cord-104268-q1jx0n0l	240	17	by	by	IN
cord-104268-q1jx0n0l	240	18	using	use	VBG
cord-104268-q1jx0n0l	240	19	a	a	DT
cord-104268-q1jx0n0l	240	20	K1	K1	NNP
cord-104268-q1jx0n0l	240	21	killer	killer	NN
cord-104268-q1jx0n0l	240	22	toxin	toxin	NN
cord-104268-q1jx0n0l	240	23	sensitivity	sensitivity	NN
cord-104268-q1jx0n0l	240	24	assay	assay	NN
cord-104268-q1jx0n0l	240	25	(	(	-LRB-
cord-104268-q1jx0n0l	240	26	Fig	Fig	NNP
cord-104268-q1jx0n0l	240	27	.	.	.
cord-104268-q1jx0n0l	240	28	9	9	CD
cord-104268-q1jx0n0l	240	29	)	)	-RRB-
cord-104268-q1jx0n0l	240	30	.	.	.
cord-104268-q1jx0n0l	241	1	K1	K1	NNP
cord-104268-q1jx0n0l	241	2	killer	killer	NN
cord-104268-q1jx0n0l	241	3	yeast	yeast	NN
cord-104268-q1jx0n0l	241	4	strains	strain	NNS
cord-104268-q1jx0n0l	241	5	secrete	secrete	VBP
cord-104268-q1jx0n0l	241	6	a	a	DT
cord-104268-q1jx0n0l	241	7	small	small	JJ
cord-104268-q1jx0n0l	241	8	pore	pore	NN
cord-104268-q1jx0n0l	241	9	-	-	HYPH
cord-104268-q1jx0n0l	241	10	forming	form	VBG
cord-104268-q1jx0n0l	241	11	toxin	toxin	NN
cord-104268-q1jx0n0l	241	12	that	that	WDT
cord-104268-q1jx0n0l	241	13	requires	require	VBZ
cord-104268-q1jx0n0l	241	14	a	a	DT
cord-104268-q1jx0n0l	241	15	cell	cell	NN
cord-104268-q1jx0n0l	241	16	-	-	HYPH
cord-104268-q1jx0n0l	241	17	wall	wall	NN
cord-104268-q1jx0n0l	241	18	receptor	receptor	NN
cord-104268-q1jx0n0l	241	19	for	for	IN
cord-104268-q1jx0n0l	241	20	function	function	NN
cord-104268-q1jx0n0l	241	21	(	(	-LRB-
cord-104268-q1jx0n0l	241	22	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	241	23	,	,	,
cord-104268-q1jx0n0l	241	24	1991	1991	CD
cord-104268-q1jx0n0l	241	25	)	)	-RRB-
cord-104268-q1jx0n0l	241	26	.	.	.
cord-104268-q1jx0n0l	242	1	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	243	1	null	null	JJ
cord-104268-q1jx0n0l	243	2	mutations	mutation	NNS
cord-104268-q1jx0n0l	243	3	lead	lead	VBP
cord-104268-q1jx0n0l	243	4	to	to	IN
cord-104268-q1jx0n0l	243	5	shorter	short	JJR
cord-104268-q1jx0n0l	243	6	mannose	mannose	NN
cord-104268-q1jx0n0l	243	7	chains	chain	NNS
cord-104268-q1jx0n0l	243	8	on	on	IN
cord-104268-q1jx0n0l	243	9	cell	cell	NN
cord-104268-q1jx0n0l	243	10	-	-	HYPH
cord-104268-q1jx0n0l	243	11	wall	wall	NN
cord-104268-q1jx0n0l	243	12	mannoproreins	mannoprorein	NNS
cord-104268-q1jx0n0l	243	13	disrupting	disrupt	VBG
cord-104268-q1jx0n0l	243	14	the	the	DT
cord-104268-q1jx0n0l	243	15	toxin	toxin	NN
cord-104268-q1jx0n0l	243	16	receptor	receptor	NN
cord-104268-q1jx0n0l	243	17	and	and	CC
cord-104268-q1jx0n0l	243	18	leading	lead	VBG
cord-104268-q1jx0n0l	243	19	to	to	IN
cord-104268-q1jx0n0l	243	20	resistance	resistance	NN
cord-104268-q1jx0n0l	243	21	(	(	-LRB-
cord-104268-q1jx0n0l	243	22	Hill	Hill	NNP
cord-104268-q1jx0n0l	243	23	et	et	NNP
cord-104268-q1jx0n0l	243	24	al	al	NNP
cord-104268-q1jx0n0l	243	25	.	.	NNP
cord-104268-q1jx0n0l	243	26	,	,	,
cord-104268-q1jx0n0l	243	27	1992	1992	CD
cord-104268-q1jx0n0l	243	28	;	;	.
cord-104268-q1jx0n0l	244	1	H/~usler	H/~usler	NNP
cord-104268-q1jx0n0l	244	2	et	et	NNP
cord-104268-q1jx0n0l	244	3	al	al	NNP
cord-104268-q1jx0n0l	244	4	.	.	NNP
cord-104268-q1jx0n0l	244	5	,	,	,
cord-104268-q1jx0n0l	244	6	1992	1992	CD
cord-104268-q1jx0n0l	244	7	)	)	-RRB-
cord-104268-q1jx0n0l	244	8	.	.	.
cord-104268-q1jx0n0l	245	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	245	2	cells	cell	NNS
cord-104268-q1jx0n0l	245	3	containing	contain	VBG
cord-104268-q1jx0n0l	245	4	different	different	JJ
cord-104268-q1jx0n0l	245	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	245	6	proteins	protein	NNS
cord-104268-q1jx0n0l	245	7	were	be	VBD
cord-104268-q1jx0n0l	245	8	assayed	assay	VBN
cord-104268-q1jx0n0l	245	9	for	for	IN
cord-104268-q1jx0n0l	245	10	killer	killer	NN
cord-104268-q1jx0n0l	245	11	toxin	toxin	NN
cord-104268-q1jx0n0l	245	12	sensitivity	sensitivity	NN
cord-104268-q1jx0n0l	245	13	and	and	CC
cord-104268-q1jx0n0l	245	14	comparisons	comparison	NNS
cord-104268-q1jx0n0l	245	15	were	be	VBD
cord-104268-q1jx0n0l	245	16	made	make	VBN
cord-104268-q1jx0n0l	245	17	between	between	IN
cord-104268-q1jx0n0l	245	18	a	a	DT
cord-104268-q1jx0n0l	245	19	sensitive	sensitive	JJ
cord-104268-q1jx0n0l	245	20	wild	wild	JJ
cord-104268-q1jx0n0l	245	21	-	-	HYPH
cord-104268-q1jx0n0l	245	22	type	type	NN
cord-104268-q1jx0n0l	245	23	strain	strain	NN
cord-104268-q1jx0n0l	245	24	,	,	,
cord-104268-q1jx0n0l	245	25	a	a	DT
cord-104268-q1jx0n0l	245	26	resistant	resistant	JJ
cord-104268-q1jx0n0l	245	27	kre2	kre2	NN
cord-104268-q1jx0n0l	245	28	null	null	JJ
cord-104268-q1jx0n0l	245	29	strain	strain	NN
cord-104268-q1jx0n0l	245	30	,	,	,
cord-104268-q1jx0n0l	245	31	and	and	CC
cord-104268-q1jx0n0l	245	32	the	the	DT
cord-104268-q1jx0n0l	245	33	null	null	JJ
cord-104268-q1jx0n0l	245	34	strain	strain	NN
cord-104268-q1jx0n0l	245	35	containing	contain	VBG
cord-104268-q1jx0n0l	245	36	plasmids	plasmid	NNS
cord-104268-q1jx0n0l	245	37	bearing	bear	VBG
cord-104268-q1jx0n0l	245	38	wild	wild	JJ
cord-104268-q1jx0n0l	245	39	-	-	HYPH
cord-104268-q1jx0n0l	245	40	type	type	NN
cord-104268-q1jx0n0l	245	41	KRE2	kre2	NN
cord-104268-q1jx0n0l	245	42	or	or	CC
cord-104268-q1jx0n0l	245	43	different	different	JJ
cord-104268-q1jx0n0l	245	44	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	245	45	constructions	construction	NNS
cord-104268-q1jx0n0l	245	46	.	.	.
cord-104268-q1jx0n0l	246	1	As	as	IN
cord-104268-q1jx0n0l	246	2	can	can	MD
cord-104268-q1jx0n0l	246	3	be	be	VB
cord-104268-q1jx0n0l	246	4	seen	see	VBN
cord-104268-q1jx0n0l	246	5	in	in	IN
cord-104268-q1jx0n0l	246	6	Fig	Fig	NNP
cord-104268-q1jx0n0l	246	7	.	.	.
cord-104268-q1jx0n0l	247	1	9	9	LS
cord-104268-q1jx0n0l	247	2	,	,	,
cord-104268-q1jx0n0l	247	3	the	the	DT
cord-104268-q1jx0n0l	247	4	kre2	kre2	NNP
cord-104268-q1jx0n0l	247	5	null	null	JJ
cord-104268-q1jx0n0l	247	6	mutant	mutant	NN
cord-104268-q1jx0n0l	247	7	is	be	VBZ
cord-104268-q1jx0n0l	247	8	toxin	toxin	NN
cord-104268-q1jx0n0l	247	9	resistant	resistant	JJ
cord-104268-q1jx0n0l	247	10	,	,	,
cord-104268-q1jx0n0l	247	11	showing	show	VBG
cord-104268-q1jx0n0l	247	12	no	no	DT
cord-104268-q1jx0n0l	247	13	killing	killing	NN
cord-104268-q1jx0n0l	247	14	zone	zone	NN
cord-104268-q1jx0n0l	247	15	,	,	,
cord-104268-q1jx0n0l	247	16	but	but	CC
cord-104268-q1jx0n0l	247	17	the	the	DT
cord-104268-q1jx0n0l	247	18	same	same	JJ
cord-104268-q1jx0n0l	247	19	strain	strain	NN
cord-104268-q1jx0n0l	247	20	containing	contain	VBG
cord-104268-q1jx0n0l	247	21	the	the	DT
cord-104268-q1jx0n0l	247	22	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	247	23	gene	gene	NN
cord-104268-q1jx0n0l	247	24	,	,	,
cord-104268-q1jx0n0l	247	25	or	or	CC
cord-104268-q1jx0n0l	247	26	expressing	express	VBG
cord-104268-q1jx0n0l	247	27	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	247	28	or	or	CC
cord-104268-q1jx0n0l	247	29	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	247	30	displays	display	VBZ
cord-104268-q1jx0n0l	247	31	a	a	DT
cord-104268-q1jx0n0l	247	32	large	large	JJ
cord-104268-q1jx0n0l	247	33	clear	clear	JJ
cord-104268-q1jx0n0l	247	34	killing	killing	NN
cord-104268-q1jx0n0l	247	35	zone	zone	NN
cord-104268-q1jx0n0l	247	36	of	of	IN
cord-104268-q1jx0n0l	247	37	,	,	,
cord-104268-q1jx0n0l	247	38	-~20	-~20	:
cord-104268-q1jx0n0l	247	39	mm	mm	NNS
cord-104268-q1jx0n0l	247	40	similar	similar	JJ
cord-104268-q1jx0n0l	247	41	to	to	IN
cord-104268-q1jx0n0l	247	42	wild	wild	JJ
cord-104268-q1jx0n0l	247	43	-	-	HYPH
cord-104268-q1jx0n0l	247	44	type	type	NN
cord-104268-q1jx0n0l	247	45	cells	cell	NNS
cord-104268-q1jx0n0l	247	46	consistent	consistent	JJ
cord-104268-q1jx0n0l	247	47	with	with	IN
cord-104268-q1jx0n0l	247	48	correct	correct	JJ
cord-104268-q1jx0n0l	247	49	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	247	50	localization	localization	NN
cord-104268-q1jx0n0l	247	51	.	.	.
cord-104268-q1jx0n0l	248	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	248	2	protein	protein	NN
cord-104268-q1jx0n0l	248	3	KD	KD	NNP
cord-104268-q1jx0n0l	248	4	-	-	HYPH
cord-104268-q1jx0n0l	248	5	K	K	NNP
cord-104268-q1jx0n0l	248	6	has	have	VBZ
cord-104268-q1jx0n0l	248	7	also	also	RB
cord-104268-q1jx0n0l	248	8	been	be	VBN
cord-104268-q1jx0n0l	248	9	shown	show	VBN
cord-104268-q1jx0n0l	248	10	to	to	TO
cord-104268-q1jx0n0l	248	11	be	be	VB
cord-104268-q1jx0n0l	248	12	localized	localize	VBN
cord-104268-q1jx0n0l	248	13	to	to	IN
cord-104268-q1jx0n0l	248	14	the	the	DT
cord-104268-q1jx0n0l	248	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	248	16	complex	complex	NN
cord-104268-q1jx0n0l	248	17	but	but	CC
cord-104268-q1jx0n0l	248	18	has	have	VBZ
cord-104268-q1jx0n0l	248	19	a	a	DT
cord-104268-q1jx0n0l	248	20	reduced	reduce	VBN
cord-104268-q1jx0n0l	248	21	zone	zone	NN
cord-104268-q1jx0n0l	248	22	size	size	NN
cord-104268-q1jx0n0l	248	23	(	(	-LRB-
cord-104268-q1jx0n0l	248	24	15	15	CD
cord-104268-q1jx0n0l	248	25	mm	mm	CD
cord-104268-q1jx0n0l	248	26	)	)	-RRB-
cord-104268-q1jx0n0l	249	1	likely	likely	RB
cord-104268-q1jx0n0l	249	2	a	a	DT
cord-104268-q1jx0n0l	249	3	consequence	consequence	NN
cord-104268-q1jx0n0l	249	4	of	of	IN
cord-104268-q1jx0n0l	249	5	its	-PRON-	PRP$
cord-104268-q1jx0n0l	249	6	reduced	reduced	JJ
cord-104268-q1jx0n0l	249	7	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	249	8	activity	activity	NN
cord-104268-q1jx0n0l	249	9	.	.	.
cord-104268-q1jx0n0l	250	1	The	the	DT
cord-104268-q1jx0n0l	250	2	reduced	reduced	JJ
cord-104268-q1jx0n0l	250	3	killing	killing	NN
cord-104268-q1jx0n0l	250	4	zone	zone	NN
cord-104268-q1jx0n0l	250	5	(	(	-LRB-
cord-104268-q1jx0n0l	250	6	10	10	CD
cord-104268-q1jx0n0l	250	7	mm	mm	NN
cord-104268-q1jx0n0l	250	8	)	)	-RRB-
cord-104268-q1jx0n0l	250	9	of	of	IN
cord-104268-q1jx0n0l	250	10	cells	cell	NNS
cord-104268-q1jx0n0l	250	11	expressing	express	VBG
cord-104268-q1jx0n0l	250	12	DKKK	DKKK	NNP
cord-104268-q1jx0n0l	250	13	which	which	WDT
cord-104268-q1jx0n0l	250	14	has	have	VBZ
cord-104268-q1jx0n0l	250	15	wild	wild	JJ
cord-104268-q1jx0n0l	250	16	-	-	HYPH
cord-104268-q1jx0n0l	250	17	type	type	NN
cord-104268-q1jx0n0l	250	18	enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	250	19	activity	activity	NN
cord-104268-q1jx0n0l	250	20	in	in	IN
cord-104268-q1jx0n0l	250	21	vitro	vitro	FW
cord-104268-q1jx0n0l	250	22	indicates	indicate	VBZ
cord-104268-q1jx0n0l	250	23	that	that	IN
cord-104268-q1jx0n0l	250	24	it	-PRON-	PRP
cord-104268-q1jx0n0l	250	25	is	be	VBZ
cord-104268-q1jx0n0l	250	26	not	not	RB
cord-104268-q1jx0n0l	250	27	correctly	correctly	RB
cord-104268-q1jx0n0l	250	28	retained	retain	VBN
cord-104268-q1jx0n0l	250	29	in	in	IN
cord-104268-q1jx0n0l	250	30	the	the	DT
cord-104268-q1jx0n0l	250	31	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	250	32	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	250	33	subcompartment	subcompartment	NN
cord-104268-q1jx0n0l	250	34	.	.	.
cord-104268-q1jx0n0l	251	1	MR	MR	NNP
cord-104268-q1jx0n0l	251	2	/	/	SYM
cord-104268-q1jx0n0l	251	3	KKK	KKK	NNP
cord-104268-q1jx0n0l	251	4	has	have	VBZ
cord-104268-q1jx0n0l	251	5	also	also	RB
cord-104268-q1jx0n0l	251	6	been	be	VBN
cord-104268-q1jx0n0l	251	7	shown	show	VBN
cord-104268-q1jx0n0l	251	8	to	to	TO
cord-104268-q1jx0n0l	251	9	be	be	VB
cord-104268-q1jx0n0l	251	10	localized	localize	VBN
cord-104268-q1jx0n0l	251	11	to	to	IN
cord-104268-q1jx0n0l	251	12	the	the	DT
cord-104268-q1jx0n0l	251	13	vacuole	vacuole	NN
cord-104268-q1jx0n0l	251	14	,	,	,
cord-104268-q1jx0n0l	251	15	but	but	CC
cord-104268-q1jx0n0l	251	16	displays	display	VBZ
cord-104268-q1jx0n0l	251	17	an	an	DT
cord-104268-q1jx0n0l	251	18	almost	almost	RB
cord-104268-q1jx0n0l	251	19	wild	wild	JJ
cord-104268-q1jx0n0l	251	20	-	-	HYPH
cord-104268-q1jx0n0l	251	21	type	type	NN
cord-104268-q1jx0n0l	251	22	zone	zone	NN
cord-104268-q1jx0n0l	251	23	size	size	NN
cord-104268-q1jx0n0l	251	24	(	(	-LRB-
cord-104268-q1jx0n0l	251	25	17.5	17.5	CD
cord-104268-q1jx0n0l	251	26	mm	mm	NNP
cord-104268-q1jx0n0l	251	27	)	)	-RRB-
cord-104268-q1jx0n0l	251	28	.	.	.
cord-104268-q1jx0n0l	252	1	Its	-PRON-	PRP$
cord-104268-q1jx0n0l	252	2	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	252	3	domain	domain	NN
cord-104268-q1jx0n0l	252	4	appears	appear	VBZ
cord-104268-q1jx0n0l	252	5	to	to	TO
cord-104268-q1jx0n0l	252	6	have	have	VB
cord-104268-q1jx0n0l	252	7	a	a	DT
cord-104268-q1jx0n0l	252	8	normal	normal	JJ
cord-104268-q1jx0n0l	252	9	conformation	conformation	NN
cord-104268-q1jx0n0l	252	10	as	as	IN
cord-104268-q1jx0n0l	252	11	it	-PRON-	PRP
cord-104268-q1jx0n0l	252	12	possesses	possess	VBZ
cord-104268-q1jx0n0l	252	13	wild	wild	JJ
cord-104268-q1jx0n0l	252	14	-	-	HYPH
cord-104268-q1jx0n0l	252	15	type	type	NN
cord-104268-q1jx0n0l	252	16	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	252	17	activity	activity	NN
cord-104268-q1jx0n0l	252	18	,	,	,
cord-104268-q1jx0n0l	252	19	but	but	CC
cord-104268-q1jx0n0l	252	20	it	-PRON-	PRP
cord-104268-q1jx0n0l	252	21	is	be	VBZ
cord-104268-q1jx0n0l	252	22	partially	partially	RB
cord-104268-q1jx0n0l	252	23	retained	retain	VBN
cord-104268-q1jx0n0l	252	24	in	in	IN
cord-104268-q1jx0n0l	252	25	the	the	DT
cord-104268-q1jx0n0l	252	26	ER	ER	NNP
cord-104268-q1jx0n0l	252	27	(	(	-LRB-
cord-104268-q1jx0n0l	252	28	see	see	VB
cord-104268-q1jx0n0l	252	29	above	above	RB
cord-104268-q1jx0n0l	252	30	)	)	-RRB-
cord-104268-q1jx0n0l	252	31	.	.	.
cord-104268-q1jx0n0l	253	1	Thus	thus	RB
cord-104268-q1jx0n0l	253	2	,	,	,
cord-104268-q1jx0n0l	253	3	MR	MR	NNP
cord-104268-q1jx0n0l	253	4	/	/	SYM
cord-104268-q1jx0n0l	253	5	KKK	KKK	NNP
cord-104268-q1jx0n0l	253	6	while	while	IN
cord-104268-q1jx0n0l	253	7	slowly	slowly	RB
cord-104268-q1jx0n0l	253	8	passing	pass	VBG
cord-104268-q1jx0n0l	253	9	through	through	IN
cord-104268-q1jx0n0l	253	10	the	the	DT
cord-104268-q1jx0n0l	253	11	secretory	secretory	JJ
cord-104268-q1jx0n0l	253	12	pathway	pathway	NN
cord-104268-q1jx0n0l	253	13	en	en	IN
cord-104268-q1jx0n0l	253	14	route	route	NN
cord-104268-q1jx0n0l	253	15	to	to	IN
cord-104268-q1jx0n0l	253	16	the	the	DT
cord-104268-q1jx0n0l	253	17	vacuole	vacuole	NN
cord-104268-q1jx0n0l	253	18	,	,	,
cord-104268-q1jx0n0l	253	19	is	be	VBZ
cord-104268-q1jx0n0l	253	20	likely	likely	RB
cord-104268-q1jx0n0l	253	21	able	able	JJ
cord-104268-q1jx0n0l	253	22	to	to	TO
cord-104268-q1jx0n0l	253	23	correctly	correctly	RB
cord-104268-q1jx0n0l	253	24	mannosylate	mannosylate	VB
cord-104268-q1jx0n0l	253	25	cell	cell	NN
cord-104268-q1jx0n0l	253	26	-	-	HYPH
cord-104268-q1jx0n0l	253	27	wall	wall	NN
cord-104268-q1jx0n0l	253	28	proteins	protein	NNS
cord-104268-q1jx0n0l	253	29	.	.	.
cord-104268-q1jx0n0l	254	1	Finally	finally	RB
cord-104268-q1jx0n0l	254	2	,	,	,
cord-104268-q1jx0n0l	254	3	these	these	DT
cord-104268-q1jx0n0l	254	4	results	result	NNS
cord-104268-q1jx0n0l	254	5	indicate	indicate	VBP
cord-104268-q1jx0n0l	254	6	that	that	IN
cord-104268-q1jx0n0l	254	7	for	for	IN
cord-104268-q1jx0n0l	254	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	254	9	the	the	DT
cord-104268-q1jx0n0l	254	10	default	default	NN
cord-104268-q1jx0n0l	254	11	compartment	compartment	NN
cord-104268-q1jx0n0l	254	12	for	for	IN
cord-104268-q1jx0n0l	254	13	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	254	14	is	be	VBZ
cord-104268-q1jx0n0l	254	15	the	the	DT
cord-104268-q1jx0n0l	254	16	vacuole	vacuole	NN
cord-104268-q1jx0n0l	254	17	,	,	,
cord-104268-q1jx0n0l	254	18	a	a	DT
cord-104268-q1jx0n0l	254	19	conclusion	conclusion	NN
cord-104268-q1jx0n0l	254	20	previously	previously	RB
cord-104268-q1jx0n0l	254	21	reached	reach	VBN
cord-104268-q1jx0n0l	254	22	for	for	IN
cord-104268-q1jx0n0l	254	23	late	late	JJ
cord-104268-q1jx0n0l	254	24	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	254	25	membrane	membrane	NN
cord-104268-q1jx0n0l	254	26	proteins	protein	NNS
cord-104268-q1jx0n0l	254	27	(	(	-LRB-
cord-104268-q1jx0n0l	254	28	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	254	29	and	and	CC
cord-104268-q1jx0n0l	254	30	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	254	31	,	,	,
cord-104268-q1jx0n0l	254	32	1992	1992	CD
cord-104268-q1jx0n0l	254	33	;	;	:
cord-104268-q1jx0n0l	254	34	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	254	35	et	et	NNP
cord-104268-q1jx0n0l	254	36	al	al	NNP
cord-104268-q1jx0n0l	254	37	.	.	NNP
cord-104268-q1jx0n0l	254	38	,	,	,
cord-104268-q1jx0n0l	254	39	1992	1992	CD
cord-104268-q1jx0n0l	254	40	;	;	:
cord-104268-q1jx0n0l	254	41	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	254	42	et	et	NNP
cord-104268-q1jx0n0l	254	43	al	al	NNP
cord-104268-q1jx0n0l	254	44	.	.	.
cord-104268-q1jx0n0l	255	1	,	,	,
cord-104268-q1jx0n0l	255	2	1992	1992	CD
cord-104268-q1jx0n0l	255	3	;	;	:
cord-104268-q1jx0n0l	255	4	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	255	5	et	et	NNP
cord-104268-q1jx0n0l	255	6	al	al	NNP
cord-104268-q1jx0n0l	255	7	.	.	NNP
cord-104268-q1jx0n0l	255	8	,	,	,
cord-104268-q1jx0n0l	255	9	1993	1993	CD
cord-104268-q1jx0n0l	255	10	)	)	-RRB-
cord-104268-q1jx0n0l	255	11	and	and	CC
cord-104268-q1jx0n0l	255	12	some	some	DT
cord-104268-q1jx0n0l	255	13	ER	ER	NNP
cord-104268-q1jx0n0l	255	14	membrane	membrane	NN
cord-104268-q1jx0n0l	255	15	proteins	protein	NNS
cord-104268-q1jx0n0l	256	1	(	(	-LRB-
cord-104268-q1jx0n0l	256	2	Gaynor	Gaynor	NNP
cord-104268-q1jx0n0l	256	3	et	et	NNP
cord-104268-q1jx0n0l	256	4	al	al	NNP
cord-104268-q1jx0n0l	256	5	.	.	.
cord-104268-q1jx0n0l	257	1	,	,	,
cord-104268-q1jx0n0l	257	2	1994	1994	CD
cord-104268-q1jx0n0l	257	3	;	;	:
cord-104268-q1jx0n0l	257	4	Hill	Hill	NNP
cord-104268-q1jx0n0l	257	5	and	and	CC
cord-104268-q1jx0n0l	257	6	Stevens	Stevens	NNP
cord-104268-q1jx0n0l	257	7	,	,	,
cord-104268-q1jx0n0l	257	8	1994	1994	CD
cord-104268-q1jx0n0l	257	9	)	)	-RRB-
cord-104268-q1jx0n0l	257	10	.	.	.
cord-104268-q1jx0n0l	258	1	To	to	TO
cord-104268-q1jx0n0l	258	2	determine	determine	VB
cord-104268-q1jx0n0l	258	3	which	which	WDT
cord-104268-q1jx0n0l	258	4	regions	region	NNS
cord-104268-q1jx0n0l	258	5	of	of	IN
cord-104268-q1jx0n0l	258	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	258	7	are	be	VBP
cord-104268-q1jx0n0l	258	8	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	258	9	to	to	TO
cord-104268-q1jx0n0l	258	10	target	target	VB
cord-104268-q1jx0n0l	258	11	a	a	DT
cord-104268-q1jx0n0l	258	12	reporter	reporter	NN
cord-104268-q1jx0n0l	258	13	protein	protein	NN
cord-104268-q1jx0n0l	258	14	to	to	IN
cord-104268-q1jx0n0l	258	15	the	the	DT
cord-104268-q1jx0n0l	258	16	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	258	17	complex	complex	NN
cord-104268-q1jx0n0l	258	18	,	,	,
cord-104268-q1jx0n0l	258	19	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	258	20	proteins	protein	NNS
cord-104268-q1jx0n0l	258	21	KKP	KKP	NNP
cord-104268-q1jx0n0l	258	22	and	and	CC
cord-104268-q1jx0n0l	258	23	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	258	24	were	be	VBD
cord-104268-q1jx0n0l	258	25	constructed	construct	VBN
cord-104268-q1jx0n0l	258	26	(	(	-LRB-
cord-104268-q1jx0n0l	258	27	see	see	VB
cord-104268-q1jx0n0l	258	28	Fig	Fig	NNP
cord-104268-q1jx0n0l	258	29	.	.	NNP
cord-104268-q1jx0n0l	258	30	6	6	CD
cord-104268-q1jx0n0l	258	31	and	and	CC
cord-104268-q1jx0n0l	258	32	Materials	Materials	NNPS
cord-104268-q1jx0n0l	258	33	and	and	CC
cord-104268-q1jx0n0l	258	34	Methods	method	NNS
cord-104268-q1jx0n0l	258	35	for	for	IN
cord-104268-q1jx0n0l	258	36	details	detail	NNS
cord-104268-q1jx0n0l	258	37	)	)	-RRB-
cord-104268-q1jx0n0l	258	38	.	.	.
cord-104268-q1jx0n0l	259	1	KKP	KKP	NNP
cord-104268-q1jx0n0l	259	2	consists	consist	VBZ
cord-104268-q1jx0n0l	259	3	of	of	IN
cord-104268-q1jx0n0l	259	4	the	the	DT
cord-104268-q1jx0n0l	259	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	259	6	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	259	7	and	and	CC
cord-104268-q1jx0n0l	259	8	membrane	membrane	NN
cord-104268-q1jx0n0l	259	9	-	-	HYPH
cord-104268-q1jx0n0l	259	10	spanning	span	VBG
cord-104268-q1jx0n0l	259	11	domains	domain	NNS
cord-104268-q1jx0n0l	259	12	fused	fuse	VBN
cord-104268-q1jx0n0l	259	13	to	to	IN
cord-104268-q1jx0n0l	259	14	the	the	DT
cord-104268-q1jx0n0l	259	15	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	259	16	luminal	luminal	JJ
cord-104268-q1jx0n0l	259	17	domain	domain	NN
cord-104268-q1jx0n0l	259	18	.	.	.
cord-104268-q1jx0n0l	260	1	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	260	2	contains	contain	VBZ
cord-104268-q1jx0n0l	260	3	the	the	DT
cord-104268-q1jx0n0l	260	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	260	5	NH2	NH2	NNP
cord-104268-q1jx0n0l	260	6	terminus	terminus	NN
cord-104268-q1jx0n0l	260	7	,	,	,
cord-104268-q1jx0n0l	260	8	TMD	TMD	NNP
cord-104268-q1jx0n0l	260	9	,	,	,
cord-104268-q1jx0n0l	260	10	and	and	CC
cord-104268-q1jx0n0l	260	11	a	a	DT
cord-104268-q1jx0n0l	260	12	segment	segment	NN
cord-104268-q1jx0n0l	260	13	encompassing	encompass	VBG
cord-104268-q1jx0n0l	260	14	the	the	DT
cord-104268-q1jx0n0l	260	15	first	first	JJ
cord-104268-q1jx0n0l	260	16	36	36	CD
cord-104268-q1jx0n0l	260	17	amino	amino	NN
cord-104268-q1jx0n0l	260	18	acid	acid	NN
cord-104268-q1jx0n0l	260	19	residues	residue	NNS
cord-104268-q1jx0n0l	260	20	of	of	IN
cord-104268-q1jx0n0l	260	21	the	the	DT
cord-104268-q1jx0n0l	260	22	stem	stem	NN
cord-104268-q1jx0n0l	260	23	domain	domain	NN
cord-104268-q1jx0n0l	260	24	fused	fuse	VBN
cord-104268-q1jx0n0l	260	25	to	to	IN
cord-104268-q1jx0n0l	260	26	and	and	CC
cord-104268-q1jx0n0l	260	27	containing	contain	VBG
cord-104268-q1jx0n0l	260	28	the	the	DT
cord-104268-q1jx0n0l	260	29	different	different	JJ
cord-104268-q1jx0n0l	260	30	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	260	31	constructions	construction	NNS
cord-104268-q1jx0n0l	260	32	on	on	IN
cord-104268-q1jx0n0l	260	33	YEp352	YEp352	NNS
cord-104268-q1jx0n0l	260	34	were	be	VBD
cord-104268-q1jx0n0l	260	35	fixed	fix	VBN
cord-104268-q1jx0n0l	260	36	,	,	,
cord-104268-q1jx0n0l	260	37	spheroplasted	spheroplaste	VBN
cord-104268-q1jx0n0l	260	38	,	,	,
cord-104268-q1jx0n0l	260	39	attached	attach	VBN
cord-104268-q1jx0n0l	260	40	to	to	IN
cord-104268-q1jx0n0l	260	41	polylysine	polylysine	NN
cord-104268-q1jx0n0l	260	42	-	-	HYPH
cord-104268-q1jx0n0l	260	43	coated	coat	VBN
cord-104268-q1jx0n0l	260	44	glass	glass	NN
cord-104268-q1jx0n0l	260	45	slides	slide	NNS
cord-104268-q1jx0n0l	260	46	,	,	,
cord-104268-q1jx0n0l	260	47	and	and	CC
cord-104268-q1jx0n0l	260	48	incubated	incubate	VBN
cord-104268-q1jx0n0l	260	49	with	with	IN
cord-104268-q1jx0n0l	260	50	anti	anti	JJ
cord-104268-q1jx0n0l	260	51	-	-	JJ
cord-104268-q1jx0n0l	260	52	Kre2p	kre2p	JJ
cord-104268-q1jx0n0l	260	53	antibodies	antibody	NNS
cord-104268-q1jx0n0l	260	54	which	which	WDT
cord-104268-q1jx0n0l	260	55	was	be	VBD
cord-104268-q1jx0n0l	260	56	followed	follow	VBN
cord-104268-q1jx0n0l	260	57	by	by	IN
cord-104268-q1jx0n0l	260	58	a	a	DT
cord-104268-q1jx0n0l	260	59	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	260	60	incubation	incubation	NN
cord-104268-q1jx0n0l	260	61	with	with	IN
cord-104268-q1jx0n0l	260	62	a	a	DT
cord-104268-q1jx0n0l	260	63	FITC	FITC	NNP
cord-104268-q1jx0n0l	260	64	-	-	HYPH
cord-104268-q1jx0n0l	260	65	coupled	couple	VBN
cord-104268-q1jx0n0l	260	66	anti	anti	JJ
cord-104268-q1jx0n0l	260	67	-	-	JJ
cord-104268-q1jx0n0l	260	68	rabbit	rabbit	JJ
cord-104268-q1jx0n0l	260	69	secondary	secondary	JJ
cord-104268-q1jx0n0l	260	70	Ab	ab	NN
cord-104268-q1jx0n0l	260	71	and	and	CC
cord-104268-q1jx0n0l	260	72	DAPI	DAPI	NNP
cord-104268-q1jx0n0l	260	73	.	.	.
cord-104268-q1jx0n0l	261	1	Colocalization	Colocalization	NNP
cord-104268-q1jx0n0l	261	2	of	of	IN
cord-104268-q1jx0n0l	261	3	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	261	4	localized	localize	VBN
cord-104268-q1jx0n0l	261	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	261	6	proteins	protein	NNS
cord-104268-q1jx0n0l	261	7	with	with	IN
cord-104268-q1jx0n0l	261	8	the	the	DT
cord-104268-q1jx0n0l	261	9	yeast	yeast	NN
cord-104268-q1jx0n0l	261	10	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	261	11	membrane	membrane	NN
cord-104268-q1jx0n0l	261	12	H÷-ATPase	H÷-ATPase	NNP
cord-104268-q1jx0n0l	261	13	was	be	VBD
cord-104268-q1jx0n0l	261	14	achieved	achieve	VBN
cord-104268-q1jx0n0l	261	15	by	by	IN
cord-104268-q1jx0n0l	261	16	coincubation	coincubation	NN
cord-104268-q1jx0n0l	261	17	of	of	IN
cord-104268-q1jx0n0l	261	18	fixed	fix	VBN
cord-104268-q1jx0n0l	261	19	cells	cell	NNS
cord-104268-q1jx0n0l	261	20	with	with	IN
cord-104268-q1jx0n0l	261	21	mAb	mab	NN
cord-104268-q1jx0n0l	261	22	13Dll	13dll	CD
cord-104268-q1jx0n0l	261	23	(	(	-LRB-
cord-104268-q1jx0n0l	261	24	Kane	Kane	NNP
cord-104268-q1jx0n0l	261	25	et	et	NNP
cord-104268-q1jx0n0l	261	26	al	al	NNP
cord-104268-q1jx0n0l	261	27	.	.	NNP
cord-104268-q1jx0n0l	261	28	,	,	,
cord-104268-q1jx0n0l	261	29	1992	1992	CD
cord-104268-q1jx0n0l	261	30	)	)	-RRB-
cord-104268-q1jx0n0l	261	31	with	with	IN
cord-104268-q1jx0n0l	261	32	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	261	33	incubation	incubation	NN
cord-104268-q1jx0n0l	261	34	of	of	IN
cord-104268-q1jx0n0l	261	35	treated	treat	VBN
cord-104268-q1jx0n0l	261	36	cells	cell	NNS
cord-104268-q1jx0n0l	261	37	with	with	IN
cord-104268-q1jx0n0l	261	38	Texas	Texas	NNP
cord-104268-q1jx0n0l	261	39	red	red	JJ
cord-104268-q1jx0n0l	261	40	-	-	HYPH
cord-104268-q1jx0n0l	261	41	conjugated	conjugate	VBN
cord-104268-q1jx0n0l	261	42	anti	anti	JJ
cord-104268-q1jx0n0l	261	43	-	-	JJ
cord-104268-q1jx0n0l	261	44	mouse	mouse	JJ
cord-104268-q1jx0n0l	261	45	secondary	secondary	JJ
cord-104268-q1jx0n0l	261	46	antibodies	antibody	NNS
cord-104268-q1jx0n0l	261	47	.	.	.
cord-104268-q1jx0n0l	262	1	the	the	DT
cord-104268-q1jx0n0l	262	2	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	262	3	luminal	luminal	JJ
cord-104268-q1jx0n0l	262	4	domain	domain	NN
cord-104268-q1jx0n0l	262	5	.	.	.
cord-104268-q1jx0n0l	263	1	Both	both	DT
cord-104268-q1jx0n0l	263	2	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	263	3	proteins	protein	NNS
cord-104268-q1jx0n0l	263	4	were	be	VBD
cord-104268-q1jx0n0l	263	5	first	first	RB
cord-104268-q1jx0n0l	263	6	verified	verify	VBN
cord-104268-q1jx0n0l	263	7	to	to	TO
cord-104268-q1jx0n0l	263	8	be	be	VB
cord-104268-q1jx0n0l	263	9	membrane	membrane	NN
cord-104268-q1jx0n0l	263	10	associated	associate	VBN
cord-104268-q1jx0n0l	263	11	and	and	CC
cord-104268-q1jx0n0l	263	12	properly	properly	RB
cord-104268-q1jx0n0l	263	13	glycosylated	glycosylate	VBN
cord-104268-q1jx0n0l	264	1	(	(	-LRB-
cord-104268-q1jx0n0l	264	2	data	datum	NNS
cord-104268-q1jx0n0l	264	3	not	not	RB
cord-104268-q1jx0n0l	264	4	shown	show	VBN
cord-104268-q1jx0n0l	264	5	)	)	-RRB-
cord-104268-q1jx0n0l	264	6	,	,	,
cord-104268-q1jx0n0l	264	7	indicative	indicative	JJ
cord-104268-q1jx0n0l	264	8	of	of	IN
cord-104268-q1jx0n0l	264	9	a	a	DT
cord-104268-q1jx0n0l	264	10	correct	correct	JJ
cord-104268-q1jx0n0l	264	11	type	type	NN
cord-104268-q1jx0n0l	264	12	II	II	NNP
cord-104268-q1jx0n0l	264	13	membrane	membrane	NN
cord-104268-q1jx0n0l	264	14	orientation	orientation	NN
cord-104268-q1jx0n0l	264	15	(	(	-LRB-
cord-104268-q1jx0n0l	264	16	Fig	Fig	NNP
cord-104268-q1jx0n0l	264	17	.	.	.
cord-104268-q1jx0n0l	264	18	6	6	CD
cord-104268-q1jx0n0l	264	19	)	)	-RRB-
cord-104268-q1jx0n0l	264	20	.	.	.
cord-104268-q1jx0n0l	265	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	265	2	protein	protein	NN
cord-104268-q1jx0n0l	265	3	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	265	4	was	be	VBD
cord-104268-q1jx0n0l	265	5	found	find	VBN
cord-104268-q1jx0n0l	265	6	,	,	,
cord-104268-q1jx0n0l	265	7	like	like	IN
cord-104268-q1jx0n0l	265	8	wild	wild	JJ
cord-104268-q1jx0n0l	265	9	-	-	HYPH
cord-104268-q1jx0n0l	265	10	type	type	NN
cord-104268-q1jx0n0l	265	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	265	12	,	,	,
cord-104268-q1jx0n0l	265	13	to	to	TO
cord-104268-q1jx0n0l	265	14	be	be	VB
cord-104268-q1jx0n0l	265	15	typically	typically	RB
cord-104268-q1jx0n0l	265	16	localized	localize	VBN
cord-104268-q1jx0n0l	265	17	to	to	TO
cord-104268-q1jx0n0l	265	18	punctiform	punctiform	VB
cord-104268-q1jx0n0l	265	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	265	20	structures	structure	NNS
cord-104268-q1jx0n0l	265	21	(	(	-LRB-
cord-104268-q1jx0n0l	265	22	Fig	Fig	NNP
cord-104268-q1jx0n0l	265	23	.	.	.
cord-104268-q1jx0n0l	265	24	10	10	CD
cord-104268-q1jx0n0l	265	25	)	)	-RRB-
cord-104268-q1jx0n0l	265	26	.	.	.
cord-104268-q1jx0n0l	266	1	However	however	RB
cord-104268-q1jx0n0l	266	2	,	,	,
cord-104268-q1jx0n0l	266	3	when	when	WRB
cord-104268-q1jx0n0l	266	4	the	the	DT
cord-104268-q1jx0n0l	266	5	partial	partial	JJ
cord-104268-q1jx0n0l	266	6	stem	stem	NN
cord-104268-q1jx0n0l	266	7	domain	domain	NN
cord-104268-q1jx0n0l	266	8	was	be	VBD
cord-104268-q1jx0n0l	266	9	removed	remove	VBN
cord-104268-q1jx0n0l	266	10	,	,	,
cord-104268-q1jx0n0l	266	11	the	the	DT
cord-104268-q1jx0n0l	266	12	majority	majority	NN
cord-104268-q1jx0n0l	266	13	of	of	IN
cord-104268-q1jx0n0l	266	14	the	the	DT
cord-104268-q1jx0n0l	266	15	resulting	result	VBG
cord-104268-q1jx0n0l	266	16	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	266	17	protein	protein	NN
cord-104268-q1jx0n0l	266	18	(	(	-LRB-
cord-104268-q1jx0n0l	266	19	KKP	KKP	NNP
cord-104268-q1jx0n0l	266	20	)	)	-RRB-
cord-104268-q1jx0n0l	266	21	was	be	VBD
cord-104268-q1jx0n0l	266	22	found	find	VBN
cord-104268-q1jx0n0l	266	23	to	to	TO
cord-104268-q1jx0n0l	266	24	be	be	VB
cord-104268-q1jx0n0l	266	25	localized	localize	VBN
cord-104268-q1jx0n0l	266	26	in	in	IN
cord-104268-q1jx0n0l	266	27	the	the	DT
cord-104268-q1jx0n0l	266	28	vacuole	vacuole	NN
cord-104268-q1jx0n0l	266	29	.	.	.
cord-104268-q1jx0n0l	267	1	The	the	DT
cord-104268-q1jx0n0l	267	2	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	267	3	localization	localization	NN
cord-104268-q1jx0n0l	267	4	of	of	IN
cord-104268-q1jx0n0l	267	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	267	6	protein	protein	NN
cord-104268-q1jx0n0l	267	7	KKP	KKP	NNP
cord-104268-q1jx0n0l	267	8	was	be	VBD
cord-104268-q1jx0n0l	267	9	quantitatively	quantitatively	RB
cord-104268-q1jx0n0l	267	10	scored	score	VBN
cord-104268-q1jx0n0l	267	11	(	(	-LRB-
cord-104268-q1jx0n0l	267	12	see	see	VB
cord-104268-q1jx0n0l	267	13	Materials	material	NNS
cord-104268-q1jx0n0l	267	14	and	and	CC
cord-104268-q1jx0n0l	267	15	Methods	Methods	NNPS
cord-104268-q1jx0n0l	267	16	)	)	-RRB-
cord-104268-q1jx0n0l	267	17	;	;	:
cord-104268-q1jx0n0l	267	18	70	70	CD
cord-104268-q1jx0n0l	267	19	%	%	NN
cord-104268-q1jx0n0l	267	20	of	of	IN
cord-104268-q1jx0n0l	267	21	positive	positive	JJ
cord-104268-q1jx0n0l	267	22	cells	cell	NNS
cord-104268-q1jx0n0l	267	23	displayed	display	VBD
cord-104268-q1jx0n0l	267	24	a	a	DT
cord-104268-q1jx0n0l	267	25	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	267	26	signal	signal	NN
cord-104268-q1jx0n0l	267	27	and	and	CC
cord-104268-q1jx0n0l	267	28	26	26	CD
cord-104268-q1jx0n0l	267	29	%	%	NN
cord-104268-q1jx0n0l	267	30	of	of	IN
cord-104268-q1jx0n0l	267	31	the	the	DT
cord-104268-q1jx0n0l	267	32	cells	cell	NNS
cord-104268-q1jx0n0l	267	33	had	have	VBD
cord-104268-q1jx0n0l	267	34	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	267	35	and	and	CC
cord-104268-q1jx0n0l	267	36	punctate	punctate	NN
cord-104268-q1jx0n0l	267	37	fluorescent	fluorescent	NN
cord-104268-q1jx0n0l	267	38	signals	signal	NNS
cord-104268-q1jx0n0l	267	39	(	(	-LRB-
cord-104268-q1jx0n0l	267	40	typical	typical	JJ
cord-104268-q1jx0n0l	267	41	results	result	NNS
cord-104268-q1jx0n0l	267	42	are	be	VBP
cord-104268-q1jx0n0l	267	43	shown	show	VBN
cord-104268-q1jx0n0l	267	44	in	in	IN
cord-104268-q1jx0n0l	267	45	Fig	Fig	NNP
cord-104268-q1jx0n0l	267	46	.	.	.
cord-104268-q1jx0n0l	267	47	10	10	CD
cord-104268-q1jx0n0l	267	48	)	)	-RRB-
cord-104268-q1jx0n0l	267	49	,	,	,
cord-104268-q1jx0n0l	267	50	whereas	whereas	IN
cord-104268-q1jx0n0l	267	51	4	4	CD
cord-104268-q1jx0n0l	267	52	%	%	NN
cord-104268-q1jx0n0l	267	53	showed	show	VBD
cord-104268-q1jx0n0l	267	54	exclusively	exclusively	RB
cord-104268-q1jx0n0l	267	55	punctiform	punctiform	NN
cord-104268-q1jx0n0l	267	56	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	267	57	.	.	.
cord-104268-q1jx0n0l	268	1	Thus	thus	RB
cord-104268-q1jx0n0l	268	2	,	,	,
cord-104268-q1jx0n0l	268	3	the	the	DT
cord-104268-q1jx0n0l	268	4	Kre2	Kre2	NNP
cord-104268-q1jx0n0l	268	5	protein	protein	NN
cord-104268-q1jx0n0l	268	6	region	region	NN
cord-104268-q1jx0n0l	268	7	encompassing	encompass	VBG
cord-104268-q1jx0n0l	268	8	the	the	DT
cord-104268-q1jx0n0l	268	9	NH2	NH2	NNP
cord-104268-q1jx0n0l	268	10	terminus	terminus	NN
cord-104268-q1jx0n0l	268	11	,	,	,
cord-104268-q1jx0n0l	268	12	TMD	TMD	NNP
cord-104268-q1jx0n0l	268	13	,	,	,
cord-104268-q1jx0n0l	268	14	and	and	CC
cord-104268-q1jx0n0l	268	15	partial	partial	JJ
cord-104268-q1jx0n0l	268	16	stem	stem	NN
cord-104268-q1jx0n0l	268	17	domain	domain	NN
cord-104268-q1jx0n0l	268	18	is	be	VBZ
cord-104268-q1jx0n0l	268	19	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	268	20	for	for	IN
cord-104268-q1jx0n0l	268	21	full	full	JJ
cord-104268-q1jx0n0l	268	22	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	268	23	retention	retention	NN
cord-104268-q1jx0n0l	268	24	of	of	IN
cord-104268-q1jx0n0l	268	25	a	a	DT
cord-104268-q1jx0n0l	268	26	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	268	27	protein	protein	NN
cord-104268-q1jx0n0l	268	28	.	.	.
cord-104268-q1jx0n0l	269	1	These	these	DT
cord-104268-q1jx0n0l	269	2	results	result	NNS
cord-104268-q1jx0n0l	269	3	were	be	VBD
cord-104268-q1jx0n0l	269	4	extended	extend	VBN
cord-104268-q1jx0n0l	269	5	biochemically	biochemically	RB
cord-104268-q1jx0n0l	269	6	by	by	IN
cord-104268-q1jx0n0l	269	7	examining	examine	VBG
cord-104268-q1jx0n0l	269	8	the	the	DT
cord-104268-q1jx0n0l	269	9	processing	processing	NN
cord-104268-q1jx0n0l	269	10	kinetics	kinetic	NNS
cord-104268-q1jx0n0l	269	11	of	of	IN
cord-104268-q1jx0n0l	269	12	both	both	DT
cord-104268-q1jx0n0l	269	13	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	269	14	proteins	protein	NNS
cord-104268-q1jx0n0l	269	15	.	.	.
cord-104268-q1jx0n0l	270	1	The	the	DT
cord-104268-q1jx0n0l	270	2	PhoSp	phosp	JJ
cord-104268-q1jx0n0l	270	3	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	270	4	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	270	5	is	be	VBZ
cord-104268-q1jx0n0l	270	6	activated	activate	VBN
cord-104268-q1jx0n0l	270	7	in	in	IN
cord-104268-q1jx0n0l	270	8	the	the	DT
cord-104268-q1jx0n0l	270	9	vacuole	vacuole	NN
cord-104268-q1jx0n0l	270	10	(	(	-LRB-
cord-104268-q1jx0n0l	270	11	Klionsky	Klionsky	NNP
cord-104268-q1jx0n0l	270	12	and	and	CC
cord-104268-q1jx0n0l	270	13	Emr	Emr	NNP
cord-104268-q1jx0n0l	270	14	,	,	,
cord-104268-q1jx0n0l	270	15	1989	1989	CD
cord-104268-q1jx0n0l	270	16	;	;	:
cord-104268-q1jx0n0l	270	17	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	270	18	et	et	NNP
cord-104268-q1jx0n0l	270	19	al	al	NNP
cord-104268-q1jx0n0l	270	20	.	.	NNP
cord-104268-q1jx0n0l	270	21	,	,	,
cord-104268-q1jx0n0l	270	22	1993	1993	CD
cord-104268-q1jx0n0l	270	23	)	)	-RRB-
cord-104268-q1jx0n0l	270	24	where	where	WRB
cord-104268-q1jx0n0l	270	25	the	the	DT
cord-104268-q1jx0n0l	270	26	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	270	27	luminal	luminal	JJ
cord-104268-q1jx0n0l	270	28	domain	domain	NN
cord-104268-q1jx0n0l	270	29	is	be	VBZ
cord-104268-q1jx0n0l	270	30	processed	process	VBN
cord-104268-q1jx0n0l	270	31	by	by	IN
cord-104268-q1jx0n0l	270	32	the	the	DT
cord-104268-q1jx0n0l	270	33	proteinase	proteinase	NN
cord-104268-q1jx0n0l	270	34	A	a	NN
cord-104268-q1jx0n0l	270	35	(	(	-LRB-
cord-104268-q1jx0n0l	270	36	Ammerer	Ammerer	NNP
cord-104268-q1jx0n0l	270	37	et	et	NNP
cord-104268-q1jx0n0l	270	38	al	al	NNP
cord-104268-q1jx0n0l	270	39	.	.	NNP
cord-104268-q1jx0n0l	270	40	,	,	,
cord-104268-q1jx0n0l	270	41	1986	1986	CD
cord-104268-q1jx0n0l	270	42	;	;	.
cord-104268-q1jx0n0l	271	1	Woolford	Woolford	NNP
cord-104268-q1jx0n0l	271	2	et	et	NNP
cord-104268-q1jx0n0l	271	3	al	al	NNP
cord-104268-q1jx0n0l	271	4	.	.	NNP
cord-104268-q1jx0n0l	271	5	,	,	,
cord-104268-q1jx0n0l	271	6	1986	1986	CD
cord-104268-q1jx0n0l	271	7	)	)	-RRB-
cord-104268-q1jx0n0l	271	8	.	.	.
cord-104268-q1jx0n0l	272	1	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	272	2	was	be	VBD
cord-104268-q1jx0n0l	272	3	found	find	VBN
cord-104268-q1jx0n0l	272	4	not	not	RB
cord-104268-q1jx0n0l	272	5	to	to	TO
cord-104268-q1jx0n0l	272	6	be	be	VB
cord-104268-q1jx0n0l	272	7	cleaved	cleave	VBN
cord-104268-q1jx0n0l	272	8	,	,	,
cord-104268-q1jx0n0l	272	9	whereas	whereas	IN
cord-104268-q1jx0n0l	272	10	in	in	IN
cord-104268-q1jx0n0l	272	11	contrast	contrast	NN
cord-104268-q1jx0n0l	272	12	,	,	,
cord-104268-q1jx0n0l	272	13	the	the	DT
cord-104268-q1jx0n0l	272	14	majority	majority	NN
cord-104268-q1jx0n0l	272	15	of	of	IN
cord-104268-q1jx0n0l	272	16	KKP	KKP	NNP
cord-104268-q1jx0n0l	272	17	was	be	VBD
cord-104268-q1jx0n0l	272	18	processed	process	VBN
cord-104268-q1jx0n0l	272	19	in	in	IN
cord-104268-q1jx0n0l	272	20	accord	accord	NN
cord-104268-q1jx0n0l	272	21	with	with	IN
cord-104268-q1jx0n0l	272	22	its	-PRON-	PRP$
cord-104268-q1jx0n0l	272	23	mostly	mostly	RB
cord-104268-q1jx0n0l	272	24	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	272	25	location	location	NN
cord-104268-q1jx0n0l	272	26	(	(	-LRB-
cord-104268-q1jx0n0l	272	27	data	datum	NNS
cord-104268-q1jx0n0l	272	28	not	not	RB
cord-104268-q1jx0n0l	272	29	shown	show	VBN
cord-104268-q1jx0n0l	272	30	)	)	-RRB-
cord-104268-q1jx0n0l	272	31	.	.	.
cord-104268-q1jx0n0l	273	1	Lastly	lastly	RB
cord-104268-q1jx0n0l	273	2	,	,	,
cord-104268-q1jx0n0l	273	3	to	to	TO
cord-104268-q1jx0n0l	273	4	assess	assess	VB
cord-104268-q1jx0n0l	273	5	if	if	IN
cord-104268-q1jx0n0l	273	6	the	the	DT
cord-104268-q1jx0n0l	273	7	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	273	8	TMD	TMD	NNP
cord-104268-q1jx0n0l	273	9	is	be	VBZ
cord-104268-q1jx0n0l	273	10	required	require	VBN
cord-104268-q1jx0n0l	273	11	for	for	IN
cord-104268-q1jx0n0l	273	12	proper	proper	JJ
cord-104268-q1jx0n0l	273	13	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	273	14	retention	retention	NN
cord-104268-q1jx0n0l	273	15	in	in	IN
cord-104268-q1jx0n0l	273	16	the	the	DT
cord-104268-q1jx0n0l	273	17	context	context	NN
cord-104268-q1jx0n0l	273	18	of	of	IN
cord-104268-q1jx0n0l	273	19	an	an	DT
cord-104268-q1jx0n0l	273	20	heterologous	heterologous	JJ
cord-104268-q1jx0n0l	273	21	luminal	luminal	JJ
cord-104268-q1jx0n0l	273	22	domain	domain	NN
cord-104268-q1jx0n0l	273	23	,	,	,
cord-104268-q1jx0n0l	273	24	we	-PRON-	PRP
cord-104268-q1jx0n0l	273	25	constructed	construct	VBD
cord-104268-q1jx0n0l	273	26	fusion	fusion	NN
cord-104268-q1jx0n0l	273	27	protein	protein	NN
cord-104268-q1jx0n0l	273	28	KPKP	KPKP	NNP
cord-104268-q1jx0n0l	273	29	in	in	IN
cord-104268-q1jx0n0l	273	30	which	which	WDT
cord-104268-q1jx0n0l	273	31	the	the	DT
cord-104268-q1jx0n0l	273	32	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	273	33	TMD	TMD	NNP
cord-104268-q1jx0n0l	273	34	of	of	IN
cord-104268-q1jx0n0l	273	35	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	273	36	was	be	VBD
cord-104268-q1jx0n0l	273	37	removed	remove	VBN
cord-104268-q1jx0n0l	273	38	and	and	CC
cord-104268-q1jx0n0l	273	39	substituted	substitute	VBN
cord-104268-q1jx0n0l	273	40	with	with	IN
cord-104268-q1jx0n0l	273	41	that	that	DT
cord-104268-q1jx0n0l	273	42	of	of	IN
cord-104268-q1jx0n0l	273	43	PhoSp	phosp	NN
cord-104268-q1jx0n0l	273	44	(	(	-LRB-
cord-104268-q1jx0n0l	273	45	see	see	VB
cord-104268-q1jx0n0l	273	46	Fig	Fig	NNP
cord-104268-q1jx0n0l	273	47	.	.	.
cord-104268-q1jx0n0l	273	48	6	6	CD
cord-104268-q1jx0n0l	273	49	)	)	-RRB-
cord-104268-q1jx0n0l	273	50	.	.	.
cord-104268-q1jx0n0l	274	1	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	274	2	retention	retention	NN
cord-104268-q1jx0n0l	274	3	of	of	IN
cord-104268-q1jx0n0l	274	4	this	this	DT
cord-104268-q1jx0n0l	274	5	fusion	fusion	NN
cord-104268-q1jx0n0l	274	6	protein	protein	NN
cord-104268-q1jx0n0l	274	7	would	would	MD
cord-104268-q1jx0n0l	274	8	indicate	indicate	VB
cord-104268-q1jx0n0l	274	9	that	that	IN
cord-104268-q1jx0n0l	274	10	the	the	DT
cord-104268-q1jx0n0l	274	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	274	12	NH2	NH2	NNP
cord-104268-q1jx0n0l	274	13	terminus	terminus	JJ
cord-104268-q1jx0n0l	274	14	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	274	15	and	and	CC
cord-104268-q1jx0n0l	274	16	stem	stem	NN
cord-104268-q1jx0n0l	274	17	domains	domain	NNS
cord-104268-q1jx0n0l	274	18	are	be	VBP
cord-104268-q1jx0n0l	274	19	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	274	20	to	to	TO
cord-104268-q1jx0n0l	274	21	achieve	achieve	VB
cord-104268-q1jx0n0l	274	22	correct	correct	JJ
cord-104268-q1jx0n0l	274	23	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	274	24	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	274	25	localization	localization	NN
cord-104268-q1jx0n0l	274	26	.	.	.
cord-104268-q1jx0n0l	275	1	Immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	275	2	studies	study	NNS
cord-104268-q1jx0n0l	275	3	of	of	IN
cord-104268-q1jx0n0l	275	4	KPKP	KPKP	NNP
cord-104268-q1jx0n0l	275	5	revealed	reveal	VBD
cord-104268-q1jx0n0l	275	6	that	that	IN
cord-104268-q1jx0n0l	275	7	this	this	DT
cord-104268-q1jx0n0l	275	8	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	275	9	protein	protein	NN
cord-104268-q1jx0n0l	275	10	was	be	VBD
cord-104268-q1jx0n0l	275	11	retained	retain	VBN
cord-104268-q1jx0n0l	275	12	in	in	IN
cord-104268-q1jx0n0l	275	13	the	the	DT
cord-104268-q1jx0n0l	275	14	ER	ER	NNP
cord-104268-q1jx0n0l	275	15	(	(	-LRB-
cord-104268-q1jx0n0l	275	16	data	datum	NNS
cord-104268-q1jx0n0l	275	17	not	not	RB
cord-104268-q1jx0n0l	275	18	shown	show	VBN
cord-104268-q1jx0n0l	275	19	)	)	-RRB-
cord-104268-q1jx0n0l	275	20	,	,	,
cord-104268-q1jx0n0l	275	21	and	and	CC
cord-104268-q1jx0n0l	275	22	thus	thus	RB
cord-104268-q1jx0n0l	275	23	uninformative	uninformative	JJ
cord-104268-q1jx0n0l	275	24	.	.	.
cord-104268-q1jx0n0l	276	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	276	2	is	be	VBZ
cord-104268-q1jx0n0l	276	3	part	part	NN
cord-104268-q1jx0n0l	276	4	of	of	IN
cord-104268-q1jx0n0l	276	5	the	the	DT
cord-104268-q1jx0n0l	276	6	yeast	yeast	NN
cord-104268-q1jx0n0l	276	7	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	276	8	machinery	machinery	NN
cord-104268-q1jx0n0l	276	9	and	and	CC
cord-104268-q1jx0n0l	276	10	was	be	VBD
cord-104268-q1jx0n0l	276	11	anticipated	anticipate	VBN
cord-104268-q1jx0n0l	276	12	to	to	TO
cord-104268-q1jx0n0l	276	13	be	be	VB
cord-104268-q1jx0n0l	276	14	localized	localize	VBN
cord-104268-q1jx0n0l	276	15	in	in	IN
cord-104268-q1jx0n0l	276	16	a	a	DT
cord-104268-q1jx0n0l	276	17	medial	medial	JJ
cord-104268-q1jx0n0l	276	18	-	-	HYPH
cord-104268-q1jx0n0l	276	19	Golgi	golgi	NN
cord-104268-q1jx0n0l	276	20	compartment	compartment	NN
cord-104268-q1jx0n0l	276	21	where	where	WRB
cord-104268-q1jx0n0l	276	22	N	N	NNP
cord-104268-q1jx0n0l	276	23	-	-	:
cord-104268-q1jx0n0l	276	24	and	and	CC
cord-104268-q1jx0n0l	276	25	O	o	NN
cord-104268-q1jx0n0l	276	26	-	-	HYPH
cord-104268-q1jx0n0l	276	27	modified	modify	VBN
cord-104268-q1jx0n0l	276	28	proteins	protein	NNS
cord-104268-q1jx0n0l	276	29	receive	receive	VBP
cord-104268-q1jx0n0l	276	30	al,2	al,2	NNP
cord-104268-q1jx0n0l	276	31	-	-	HYPH
cord-104268-q1jx0n0l	276	32	1inked	1inked	CD
cord-104268-q1jx0n0l	276	33	mannose	mannose	NN
cord-104268-q1jx0n0l	276	34	residues	residue	NNS
cord-104268-q1jx0n0l	276	35	(	(	-LRB-
cord-104268-q1jx0n0l	276	36	Fig	Fig	NNP
cord-104268-q1jx0n0l	276	37	.	.	NNP
cord-104268-q1jx0n0l	276	38	2	2	CD
cord-104268-q1jx0n0l	276	39	)	)	-RRB-
cord-104268-q1jx0n0l	276	40	.	.	.
cord-104268-q1jx0n0l	277	1	Evidence	evidence	NN
cord-104268-q1jx0n0l	277	2	presented	present	VBN
cord-104268-q1jx0n0l	277	3	here	here	RB
cord-104268-q1jx0n0l	277	4	is	be	VBZ
cord-104268-q1jx0n0l	277	5	consistent	consistent	JJ
cord-104268-q1jx0n0l	277	6	with	with	IN
cord-104268-q1jx0n0l	277	7	this	this	DT
cord-104268-q1jx0n0l	277	8	expectation	expectation	NN
cord-104268-q1jx0n0l	277	9	.	.	.
cord-104268-q1jx0n0l	278	1	We	-PRON-	PRP
cord-104268-q1jx0n0l	278	2	have	have	VBP
cord-104268-q1jx0n0l	278	3	demonstrated	demonstrate	VBN
cord-104268-q1jx0n0l	278	4	that	that	IN
cord-104268-q1jx0n0l	278	5	in	in	IN
cord-104268-q1jx0n0l	278	6	situ	situ	VBG
cord-104268-q1jx0n0l	278	7	detection	detection	NN
cord-104268-q1jx0n0l	278	8	of	of	IN
cord-104268-q1jx0n0l	278	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	278	10	by	by	IN
cord-104268-q1jx0n0l	278	11	indirect	indirect	JJ
cord-104268-q1jx0n0l	278	12	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	278	13	reveals	reveal	VBZ
cord-104268-q1jx0n0l	278	14	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	278	15	punctiform	punctiform	NN
cord-104268-q1jx0n0l	278	16	staining	stain	VBG
cord-104268-q1jx0n0l	278	17	typical	typical	JJ
cord-104268-q1jx0n0l	278	18	of	of	IN
cord-104268-q1jx0n0l	278	19	the	the	DT
cord-104268-q1jx0n0l	278	20	yeast	yeast	NN
cord-104268-q1jx0n0l	278	21	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	278	22	,	,	,
cord-104268-q1jx0n0l	278	23	in	in	IN
cord-104268-q1jx0n0l	278	24	agreement	agreement	NN
cord-104268-q1jx0n0l	278	25	with	with	IN
cord-104268-q1jx0n0l	278	26	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	278	27	and	and	CC
cord-104268-q1jx0n0l	278	28	Munro	Munro	NNP
cord-104268-q1jx0n0l	278	29	(	(	-LRB-
cord-104268-q1jx0n0l	278	30	1994	1994	CD
cord-104268-q1jx0n0l	278	31	)	)	-RRB-
cord-104268-q1jx0n0l	278	32	.	.	.
cord-104268-q1jx0n0l	279	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	279	2	is	be	VBZ
cord-104268-q1jx0n0l	279	3	an	an	DT
cord-104268-q1jx0n0l	279	4	al,2-mannosyltransferase	al,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	279	5	that	that	WDT
cord-104268-q1jx0n0l	279	6	receives	receive	VBZ
cord-104268-q1jx0n0l	279	7	an	an	DT
cord-104268-q1jx0n0l	279	8	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	279	9	-	-	HYPH
cord-104268-q1jx0n0l	279	10	dependent	dependent	JJ
cord-104268-q1jx0n0l	279	11	ctl,3	ctl,3	:
cord-104268-q1jx0n0l	279	12	modification	modification	NN
cord-104268-q1jx0n0l	279	13	,	,	,
cord-104268-q1jx0n0l	279	14	the	the	DT
cord-104268-q1jx0n0l	279	15	next	next	JJ
cord-104268-q1jx0n0l	279	16	sequential	sequential	JJ
cord-104268-q1jx0n0l	279	17	step	step	NN
cord-104268-q1jx0n0l	279	18	in	in	IN
cord-104268-q1jx0n0l	279	19	the	the	DT
cord-104268-q1jx0n0l	279	20	orderly	orderly	JJ
cord-104268-q1jx0n0l	279	21	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	279	22	of	of	IN
cord-104268-q1jx0n0l	279	23	yeast	yeast	NN
cord-104268-q1jx0n0l	279	24	proteins	protein	NNS
cord-104268-q1jx0n0l	279	25	.	.	.
cord-104268-q1jx0n0l	280	1	Thus	thus	RB
cord-104268-q1jx0n0l	280	2	,	,	,
cord-104268-q1jx0n0l	280	3	Kre2p	kre2p	PRP
cord-104268-q1jx0n0l	280	4	must	must	MD
cord-104268-q1jx0n0l	280	5	reach	reach	VB
cord-104268-q1jx0n0l	280	6	a	a	DT
cord-104268-q1jx0n0l	280	7	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	280	8	compartment	compartment	NN
cord-104268-q1jx0n0l	280	9	harboring	harbor	VBG
cord-104268-q1jx0n0l	280	10	the	the	DT
cord-104268-q1jx0n0l	280	11	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	280	12	cd,3-mannosyltransferase	cd,3-mannosyltransferase	XX
cord-104268-q1jx0n0l	281	1	(	(	-LRB-
cord-104268-q1jx0n0l	281	2	Fig	Fig	NNP
cord-104268-q1jx0n0l	281	3	.	.	NNP
cord-104268-q1jx0n0l	281	4	2	2	CD
cord-104268-q1jx0n0l	281	5	)	)	-RRB-
cord-104268-q1jx0n0l	281	6	.	.	.
cord-104268-q1jx0n0l	282	1	These	these	DT
cord-104268-q1jx0n0l	282	2	ed,2and	ed,2and	NNS
cord-104268-q1jx0n0l	282	3	al,3	al,3	NNP
cord-104268-q1jx0n0l	282	4	-	-	HYPH
cord-104268-q1jx0n0l	282	5	1inked	1inked	CD
cord-104268-q1jx0n0l	282	6	activities	activity	NNS
cord-104268-q1jx0n0l	282	7	could	could	MD
cord-104268-q1jx0n0l	282	8	be	be	VB
cord-104268-q1jx0n0l	282	9	colocalized	colocalize	VBN
cord-104268-q1jx0n0l	282	10	,	,	,
cord-104268-q1jx0n0l	282	11	or	or	CC
cord-104268-q1jx0n0l	282	12	,	,	,
cord-104268-q1jx0n0l	282	13	if	if	IN
cord-104268-q1jx0n0l	282	14	they	-PRON-	PRP
cord-104268-q1jx0n0l	282	15	are	be	VBP
cord-104268-q1jx0n0l	282	16	situated	situate	VBN
cord-104268-q1jx0n0l	282	17	in	in	IN
cord-104268-q1jx0n0l	282	18	two	two	CD
cord-104268-q1jx0n0l	282	19	distinct	distinct	JJ
cord-104268-q1jx0n0l	282	20	and	and	CC
cord-104268-q1jx0n0l	282	21	consecutive	consecutive	JJ
cord-104268-q1jx0n0l	282	22	cisternae	cisternae	NN
cord-104268-q1jx0n0l	282	23	,	,	,
cord-104268-q1jx0n0l	282	24	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	282	25	would	would	MD
cord-104268-q1jx0n0l	282	26	have	have	VB
cord-104268-q1jx0n0l	282	27	to	to	TO
cord-104268-q1jx0n0l	282	28	reach	reach	VB
cord-104268-q1jx0n0l	282	29	the	the	DT
cord-104268-q1jx0n0l	282	30	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	282	31	compartment	compartment	NN
cord-104268-q1jx0n0l	282	32	and	and	CC
cord-104268-q1jx0n0l	282	33	then	then	RB
cord-104268-q1jx0n0l	282	34	be	be	VB
cord-104268-q1jx0n0l	282	35	retrieved	retrieve	VBN
cord-104268-q1jx0n0l	282	36	to	to	IN
cord-104268-q1jx0n0l	282	37	its	-PRON-	PRP$
cord-104268-q1jx0n0l	282	38	own	own	JJ
cord-104268-q1jx0n0l	282	39	preceding	precede	VBG
cord-104268-q1jx0n0l	282	40	organelle	organelle	NN
cord-104268-q1jx0n0l	282	41	.	.	.
cord-104268-q1jx0n0l	283	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	283	2	and	and	CC
cord-104268-q1jx0n0l	283	3	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	283	4	have	have	VBP
cord-104268-q1jx0n0l	283	5	been	be	VBN
cord-104268-q1jx0n0l	283	6	shown	show	VBN
cord-104268-q1jx0n0l	283	7	to	to	IN
cord-104268-q1jx0n0l	283	8	colocalize	colocalize	NN
cord-104268-q1jx0n0l	283	9	to	to	IN
cord-104268-q1jx0n0l	283	10	a	a	DT
cord-104268-q1jx0n0l	283	11	considerable	considerable	JJ
cord-104268-q1jx0n0l	283	12	extent	extent	NN
cord-104268-q1jx0n0l	283	13	,	,	,
cord-104268-q1jx0n0l	283	14	placing	place	VBG
cord-104268-q1jx0n0l	283	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	283	16	in	in	IN
cord-104268-q1jx0n0l	283	17	or	or	CC
cord-104268-q1jx0n0l	283	18	close	close	RB
cord-104268-q1jx0n0l	283	19	to	to	IN
cord-104268-q1jx0n0l	283	20	the	the	DT
cord-104268-q1jx0n0l	283	21	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	283	22	medial	medial	JJ
cord-104268-q1jx0n0l	283	23	-	-	HYPH
cord-104268-q1jx0n0l	283	24	Golgi	golgi	NN
cord-104268-q1jx0n0l	283	25	compartment	compartment	NN
cord-104268-q1jx0n0l	283	26	.	.	.
cord-104268-q1jx0n0l	284	1	Consistent	consistent	JJ
cord-104268-q1jx0n0l	284	2	with	with	IN
cord-104268-q1jx0n0l	284	3	this	this	DT
cord-104268-q1jx0n0l	284	4	assignment	assignment	NN
cord-104268-q1jx0n0l	284	5	,	,	,
cord-104268-q1jx0n0l	284	6	previous	previous	JJ
cord-104268-q1jx0n0l	284	7	biochemical	biochemical	JJ
cord-104268-q1jx0n0l	284	8	studies	study	NNS
cord-104268-q1jx0n0l	284	9	provide	provide	VBP
cord-104268-q1jx0n0l	284	10	evidence	evidence	NN
cord-104268-q1jx0n0l	284	11	that	that	IN
cord-104268-q1jx0n0l	284	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	284	13	is	be	VBZ
cord-104268-q1jx0n0l	284	14	localized	localize	VBN
cord-104268-q1jx0n0l	284	15	in	in	IN
cord-104268-q1jx0n0l	284	16	a	a	DT
cord-104268-q1jx0n0l	284	17	compartment	compartment	NN
cord-104268-q1jx0n0l	284	18	distinct	distinct	JJ
cord-104268-q1jx0n0l	284	19	from	from	IN
cord-104268-q1jx0n0l	284	20	the	the	DT
cord-104268-q1jx0n0l	284	21	late	late	JJ
cord-104268-q1jx0n0l	284	22	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	284	23	.	.	.
cord-104268-q1jx0n0l	285	1	Immunoisolated	immunoisolated	JJ
cord-104268-q1jx0n0l	285	2	late	late	JJ
cord-104268-q1jx0n0l	285	3	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	285	4	organelles	organelle	NNS
cord-104268-q1jx0n0l	285	5	containing	contain	VBG
cord-104268-q1jx0n0l	285	6	the	the	DT
cord-104268-q1jx0n0l	285	7	endoproteinases	endoproteinase	NNS
cord-104268-q1jx0n0l	285	8	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	285	9	,	,	,
cord-104268-q1jx0n0l	285	10	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	285	11	,	,	,
cord-104268-q1jx0n0l	285	12	and	and	CC
cord-104268-q1jx0n0l	285	13	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	285	14	A	A	NNP
cord-104268-q1jx0n0l	285	15	were	be	VBD
cord-104268-q1jx0n0l	285	16	shown	show	VBN
cord-104268-q1jx0n0l	285	17	to	to	TO
cord-104268-q1jx0n0l	285	18	be	be	VB
cord-104268-q1jx0n0l	285	19	devoid	devoid	JJ
cord-104268-q1jx0n0l	285	20	of	of	IN
cord-104268-q1jx0n0l	285	21	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	286	1	(	(	-LRB-
cord-104268-q1jx0n0l	286	2	Bryant	Bryant	NNP
cord-104268-q1jx0n0l	286	3	and	and	CC
cord-104268-q1jx0n0l	286	4	Boyd	Boyd	NNP
cord-104268-q1jx0n0l	286	5	,	,	,
cord-104268-q1jx0n0l	286	6	1993	1993	CD
cord-104268-q1jx0n0l	286	7	)	)	-RRB-
cord-104268-q1jx0n0l	286	8	.	.	.
cord-104268-q1jx0n0l	287	1	Our	-PRON-	PRP$
cord-104268-q1jx0n0l	287	2	immunocytochemical	immunocytochemical	JJ
cord-104268-q1jx0n0l	287	3	colocalization	colocalization	NN
cord-104268-q1jx0n0l	287	4	results	result	NNS
cord-104268-q1jx0n0l	287	5	also	also	RB
cord-104268-q1jx0n0l	287	6	indicate	indicate	VBP
cord-104268-q1jx0n0l	287	7	that	that	IN
cord-104268-q1jx0n0l	287	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	287	9	is	be	VBZ
cord-104268-q1jx0n0l	287	10	not	not	RB
cord-104268-q1jx0n0l	287	11	in	in	IN
cord-104268-q1jx0n0l	287	12	the	the	DT
cord-104268-q1jx0n0l	287	13	same	same	JJ
cord-104268-q1jx0n0l	287	14	compartment	compartment	NN
cord-104268-q1jx0n0l	287	15	as	as	IN
cord-104268-q1jx0n0l	287	16	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	287	17	.	.	.
cord-104268-q1jx0n0l	288	1	The	the	DT
cord-104268-q1jx0n0l	288	2	35	35	CD
cord-104268-q1jx0n0l	288	3	%	%	NN
cord-104268-q1jx0n0l	288	4	of	of	IN
cord-104268-q1jx0n0l	288	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	288	6	and	and	CC
cord-104268-q1jx0n0l	288	7	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	288	8	punctiform	punctiform	NN
cord-104268-q1jx0n0l	288	9	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	288	10	signals	signal	NNS
cord-104268-q1jx0n0l	288	11	that	that	WDT
cord-104268-q1jx0n0l	288	12	do	do	VBP
cord-104268-q1jx0n0l	288	13	colocalize	colocalize	NN
cord-104268-q1jx0n0l	288	14	could	could	MD
cord-104268-q1jx0n0l	288	15	be	be	VB
cord-104268-q1jx0n0l	288	16	due	due	JJ
cord-104268-q1jx0n0l	288	17	to	to	IN
cord-104268-q1jx0n0l	288	18	two	two	CD
cord-104268-q1jx0n0l	288	19	or	or	CC
cord-104268-q1jx0n0l	288	20	more	more	RBR
cord-104268-q1jx0n0l	288	21	stacked	stack	VBN
cord-104268-q1jx0n0l	288	22	cisternae	cisternae	NN
cord-104268-q1jx0n0l	288	23	that	that	WDT
cord-104268-q1jx0n0l	288	24	are	be	VBP
cord-104268-q1jx0n0l	288	25	seen	see	VBN
cord-104268-q1jx0n0l	288	26	in	in	IN
cord-104268-q1jx0n0l	288	27	~40	~40	CD
cord-104268-q1jx0n0l	288	28	%	%	NN
cord-104268-q1jx0n0l	288	29	of	of	IN
cord-104268-q1jx0n0l	288	30	all	all	DT
cord-104268-q1jx0n0l	288	31	cisternae	cisternae	NN
cord-104268-q1jx0n0l	288	32	in	in	IN
cord-104268-q1jx0n0l	288	33	a	a	DT
cord-104268-q1jx0n0l	288	34	given	give	VBN
cord-104268-q1jx0n0l	288	35	yeast	yeast	NN
cord-104268-q1jx0n0l	288	36	cell	cell	NN
cord-104268-q1jx0n0l	288	37	(	(	-LRB-
cord-104268-q1jx0n0l	288	38	Preuss	Preuss	NNP
cord-104268-q1jx0n0l	288	39	et	et	FW
cord-104268-q1jx0n0l	288	40	al	al	NNP
cord-104268-q1jx0n0l	288	41	.	.	NNP
cord-104268-q1jx0n0l	288	42	,	,	,
cord-104268-q1jx0n0l	288	43	1992	1992	CD
cord-104268-q1jx0n0l	288	44	)	)	-RRB-
cord-104268-q1jx0n0l	288	45	.	.	.
cord-104268-q1jx0n0l	289	1	However	however	RB
cord-104268-q1jx0n0l	289	2	,	,	,
cord-104268-q1jx0n0l	289	3	the	the	DT
cord-104268-q1jx0n0l	289	4	possibility	possibility	NN
cord-104268-q1jx0n0l	289	5	remains	remain	VBZ
cord-104268-q1jx0n0l	289	6	that	that	IN
cord-104268-q1jx0n0l	289	7	proteins	protein	NNS
cord-104268-q1jx0n0l	289	8	from	from	IN
cord-104268-q1jx0n0l	289	9	the	the	DT
cord-104268-q1jx0n0l	289	10	medial	medial	JJ
cord-104268-q1jx0n0l	289	11	-	-	HYPH
cord-104268-q1jx0n0l	289	12	Golgi	golgi	NN
cord-104268-q1jx0n0l	289	13	(	(	-LRB-
cord-104268-q1jx0n0l	289	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	289	15	)	)	-RRB-
cord-104268-q1jx0n0l	289	16	and	and	CC
cord-104268-q1jx0n0l	289	17	from	from	IN
cord-104268-q1jx0n0l	289	18	the	the	DT
cord-104268-q1jx0n0l	289	19	late	late	JJ
cord-104268-q1jx0n0l	289	20	 	 	_SP
cord-104268-q1jx0n0l	289	21	Kre2,~	Kre2,~	NNP
cord-104268-q1jx0n0l	289	22	31	31	CD
cord-104268-q1jx0n0l	289	23	%	%	NN
cord-104268-q1jx0n0l	289	24	Figure	figure	NN
cord-104268-q1jx0n0l	289	25	9	9	CD
cord-104268-q1jx0n0l	289	26	.	.	.
cord-104268-q1jx0n0l	290	1	Activity	activity	NN
cord-104268-q1jx0n0l	290	2	of	of	IN
cord-104268-q1jx0n0l	290	3	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	290	4	proteins	protein	NNS
cord-104268-q1jx0n0l	290	5	.	.	.
cord-104268-q1jx0n0l	291	1	Enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	291	2	activity	activity	NN
cord-104268-q1jx0n0l	291	3	was	be	VBD
cord-104268-q1jx0n0l	291	4	measured	measure	VBN
cord-104268-q1jx0n0l	291	5	in	in	IN
cord-104268-q1jx0n0l	291	6	vitro	vitro	FW
cord-104268-q1jx0n0l	291	7	by	by	IN
cord-104268-q1jx0n0l	291	8	ed,2-mannosyltransferase	ed,2-mannosyltransferase	NNP
cord-104268-q1jx0n0l	292	1	assays	assay	NNS
cord-104268-q1jx0n0l	292	2	of	of	IN
cord-104268-q1jx0n0l	292	3	membrane	membrane	NN
cord-104268-q1jx0n0l	292	4	preparations	preparation	NNS
cord-104268-q1jx0n0l	292	5	,	,	,
cord-104268-q1jx0n0l	292	6	and	and	CC
cord-104268-q1jx0n0l	292	7	in	in	IN
cord-104268-q1jx0n0l	292	8	vivo	vivo	NNP
cord-104268-q1jx0n0l	292	9	mannosylation	mannosylation	NN
cord-104268-q1jx0n0l	292	10	of	of	IN
cord-104268-q1jx0n0l	292	11	cell	cell	NN
cord-104268-q1jx0n0l	292	12	-	-	HYPH
cord-104268-q1jx0n0l	292	13	wall	wall	NN
cord-104268-q1jx0n0l	292	14	proteins	protein	NNS
cord-104268-q1jx0n0l	292	15	was	be	VBD
cord-104268-q1jx0n0l	292	16	assessed	assess	VBN
cord-104268-q1jx0n0l	292	17	by	by	IN
cord-104268-q1jx0n0l	292	18	verifying	verify	VBG
cord-104268-q1jx0n0l	292	19	K1	K1	NNP
cord-104268-q1jx0n0l	292	20	killer	killer	NN
cord-104268-q1jx0n0l	292	21	toxin	toxin	NN
cord-104268-q1jx0n0l	292	22	sensitivity	sensitivity	NN
cord-104268-q1jx0n0l	292	23	in	in	IN
cord-104268-q1jx0n0l	292	24	a	a	DT
cord-104268-q1jx0n0l	292	25	wild	wild	JJ
cord-104268-q1jx0n0l	292	26	-	-	HYPH
cord-104268-q1jx0n0l	292	27	type	type	NN
cord-104268-q1jx0n0l	292	28	strain	strain	NN
cord-104268-q1jx0n0l	292	29	and	and	CC
cord-104268-q1jx0n0l	292	30	a	a	DT
cord-104268-q1jx0n0l	292	31	kre2	kre2	NN
cord-104268-q1jx0n0l	292	32	null	null	JJ
cord-104268-q1jx0n0l	292	33	mutant	mutant	NN
cord-104268-q1jx0n0l	292	34	containing	contain	VBG
cord-104268-q1jx0n0l	292	35	KRE2	kre2	JJ
cord-104268-q1jx0n0l	292	36	and	and	CC
cord-104268-q1jx0n0l	292	37	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	292	38	constructs	construct	NNS
cord-104268-q1jx0n0l	292	39	expressed	express	VBN
cord-104268-q1jx0n0l	292	40	from	from	IN
cord-104268-q1jx0n0l	292	41	the	the	DT
cord-104268-q1jx0n0l	292	42	centromeric	centromeric	RB
cord-104268-q1jx0n0l	292	43	-	-	HYPH
cord-104268-q1jx0n0l	292	44	based	base	VBN
cord-104268-q1jx0n0l	292	45	plasmid	plasmid	NN
cord-104268-q1jx0n0l	293	1	pRS316	prs316	LS
cord-104268-q1jx0n0l	294	1	(	(	-LRB-
cord-104268-q1jx0n0l	294	2	Sikorski	Sikorski	NNP
cord-104268-q1jx0n0l	294	3	and	and	CC
cord-104268-q1jx0n0l	294	4	Hieter	Hieter	NNP
cord-104268-q1jx0n0l	294	5	,	,	,
cord-104268-q1jx0n0l	294	6	1989	1989	CD
cord-104268-q1jx0n0l	294	7	)	)	-RRB-
cord-104268-q1jx0n0l	294	8	.	.	.
cord-104268-q1jx0n0l	295	1	Enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	295	2	activity	activity	NN
cord-104268-q1jx0n0l	295	3	is	be	VBZ
cord-104268-q1jx0n0l	295	4	expressed	express	VBN
cord-104268-q1jx0n0l	295	5	as	as	IN
cord-104268-q1jx0n0l	295	6	percentages	percentage	NNS
cord-104268-q1jx0n0l	295	7	of	of	IN
cord-104268-q1jx0n0l	295	8	specific	specific	JJ
cord-104268-q1jx0n0l	295	9	activity	activity	NN
cord-104268-q1jx0n0l	295	10	(	(	-LRB-
cord-104268-q1jx0n0l	295	11	mmot	mmot	NNP
cord-104268-q1jx0n0l	295	12	/	/	SYM
cord-104268-q1jx0n0l	295	13	h	h	NNP
cord-104268-q1jx0n0l	295	14	/	/	SYM
cord-104268-q1jx0n0l	295	15	mg	mg	NNP
cord-104268-q1jx0n0l	295	16	)	)	-RRB-
cord-104268-q1jx0n0l	295	17	for	for	IN
cord-104268-q1jx0n0l	295	18	each	each	DT
cord-104268-q1jx0n0l	295	19	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	295	20	protein	protein	NN
cord-104268-q1jx0n0l	295	21	.	.	.
cord-104268-q1jx0n0l	296	1	A	a	DT
cord-104268-q1jx0n0l	296	2	kre2	kre2	NN
cord-104268-q1jx0n0l	296	3	null	null	JJ
cord-104268-q1jx0n0l	296	4	strain	strain	NN
cord-104268-q1jx0n0l	296	5	expressing	express	VBG
cord-104268-q1jx0n0l	296	6	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	296	7	from	from	IN
cord-104268-q1jx0n0l	296	8	pRS316	pRS316	NNP
cord-104268-q1jx0n0l	296	9	arbitrarily	arbitrarily	RB
cord-104268-q1jx0n0l	296	10	represents	represent	VBZ
cord-104268-q1jx0n0l	296	11	100	100	CD
cord-104268-q1jx0n0l	296	12	%	%	NN
cord-104268-q1jx0n0l	296	13	specific	specific	JJ
cord-104268-q1jx0n0l	296	14	activity	activity	NN
cord-104268-q1jx0n0l	296	15	.	.	.
cord-104268-q1jx0n0l	297	1	Concentrated	concentrated	JJ
cord-104268-q1jx0n0l	297	2	K1	K1	NNP
cord-104268-q1jx0n0l	297	3	killer	killer	NN
cord-104268-q1jx0n0l	297	4	toxin	toxin	NN
cord-104268-q1jx0n0l	297	5	was	be	VBD
cord-104268-q1jx0n0l	297	6	spotted	spot	VBN
cord-104268-q1jx0n0l	297	7	on	on	IN
cord-104268-q1jx0n0l	297	8	a	a	DT
cord-104268-q1jx0n0l	297	9	lawn	lawn	NN
cord-104268-q1jx0n0l	297	10	of	of	IN
cord-104268-q1jx0n0l	297	11	~,106	~,106	ADD
cord-104268-q1jx0n0l	297	12	/	/	SYM
cord-104268-q1jx0n0l	297	13	ml	ml	IN
cord-104268-q1jx0n0l	297	14	cells	cell	NNS
cord-104268-q1jx0n0l	297	15	from	from	IN
cord-104268-q1jx0n0l	297	16	a	a	DT
cord-104268-q1jx0n0l	297	17	fresh	fresh	JJ
cord-104268-q1jx0n0l	297	18	culture	culture	NN
cord-104268-q1jx0n0l	297	19	of	of	IN
cord-104268-q1jx0n0l	297	20	each	each	DT
cord-104268-q1jx0n0l	297	21	strain	strain	NN
cord-104268-q1jx0n0l	297	22	(	(	-LRB-
cord-104268-q1jx0n0l	297	23	see	see	VB
cord-104268-q1jx0n0l	297	24	Materials	material	NNS
cord-104268-q1jx0n0l	297	25	and	and	CC
cord-104268-q1jx0n0l	297	26	Methods	Methods	NNPS
cord-104268-q1jx0n0l	297	27	)	)	-RRB-
cord-104268-q1jx0n0l	297	28	.	.	.
cord-104268-q1jx0n0l	298	1	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	298	2	(	(	-LRB-
cord-104268-q1jx0n0l	298	3	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	298	4	)	)	-RRB-
cord-104268-q1jx0n0l	298	5	are	be	VBP
cord-104268-q1jx0n0l	298	6	not	not	RB
cord-104268-q1jx0n0l	298	7	always	always	RB
cord-104268-q1jx0n0l	298	8	in	in	IN
cord-104268-q1jx0n0l	298	9	mutually	mutually	RB
cord-104268-q1jx0n0l	298	10	exclusive	exclusive	JJ
cord-104268-q1jx0n0l	298	11	cisternae	cisternae	NN
cord-104268-q1jx0n0l	298	12	and	and	CC
cord-104268-q1jx0n0l	298	13	their	-PRON-	PRP$
cord-104268-q1jx0n0l	298	14	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	298	15	distribution	distribution	NN
cord-104268-q1jx0n0l	298	16	may	may	MD
cord-104268-q1jx0n0l	298	17	partially	partially	RB
cord-104268-q1jx0n0l	298	18	overlap	overlap	VB
cord-104268-q1jx0n0l	298	19	.	.	.
cord-104268-q1jx0n0l	299	1	This	this	DT
cord-104268-q1jx0n0l	299	2	can	can	MD
cord-104268-q1jx0n0l	299	3	be	be	VB
cord-104268-q1jx0n0l	299	4	the	the	DT
cord-104268-q1jx0n0l	299	5	case	case	NN
cord-104268-q1jx0n0l	299	6	in	in	IN
cord-104268-q1jx0n0l	299	7	mammals	mammal	NNS
cord-104268-q1jx0n0l	299	8	where	where	WRB
cord-104268-q1jx0n0l	299	9	a	a	DT
cord-104268-q1jx0n0l	299	10	pair	pair	NN
cord-104268-q1jx0n0l	299	11	of	of	IN
cord-104268-q1jx0n0l	299	12	trans	trans	NNP
cord-104268-q1jx0n0l	299	13	-	-	NNP
cord-104268-q1jx0n0l	299	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	299	15	and	and	CC
cord-104268-q1jx0n0l	299	16	medial	medial	JJ
cord-104268-q1jx0n0l	299	17	-	-	HYPH
cord-104268-q1jx0n0l	299	18	Golgi	golgi	NN
cord-104268-q1jx0n0l	299	19	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	299	20	showed	show	VBD
cord-104268-q1jx0n0l	299	21	substantial	substantial	JJ
cord-104268-q1jx0n0l	299	22	overlap	overlap	NN
cord-104268-q1jx0n0l	299	23	(	(	-LRB-
cord-104268-q1jx0n0l	299	24	Nilsson	Nilsson	NNP
cord-104268-q1jx0n0l	299	25	et	et	NNP
cord-104268-q1jx0n0l	299	26	al	al	NNP
cord-104268-q1jx0n0l	299	27	.	.	NNP
cord-104268-q1jx0n0l	299	28	,	,	,
cord-104268-q1jx0n0l	299	29	1993	1993	CD
cord-104268-q1jx0n0l	299	30	)	)	-RRB-
cord-104268-q1jx0n0l	299	31	.	.	.
cord-104268-q1jx0n0l	300	1	The	the	DT
cord-104268-q1jx0n0l	300	2	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	300	3	/	/	SYM
cord-104268-q1jx0n0l	300	4	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	300	5	compartment	compartment	NN
cord-104268-q1jx0n0l	300	6	remains	remain	VBZ
cord-104268-q1jx0n0l	300	7	to	to	TO
cord-104268-q1jx0n0l	300	8	be	be	VB
cord-104268-q1jx0n0l	300	9	precisely	precisely	RB
cord-104268-q1jx0n0l	300	10	defined	define	VBN
cord-104268-q1jx0n0l	300	11	but	but	CC
cord-104268-q1jx0n0l	300	12	some	some	DT
cord-104268-q1jx0n0l	300	13	evidence	evidence	NN
cord-104268-q1jx0n0l	300	14	suggests	suggest	VBZ
cord-104268-q1jx0n0l	300	15	it	-PRON-	PRP
cord-104268-q1jx0n0l	300	16	could	could	MD
cord-104268-q1jx0n0l	300	17	be	be	VB
cord-104268-q1jx0n0l	300	18	identical	identical	JJ
cord-104268-q1jx0n0l	300	19	to	to	IN
cord-104268-q1jx0n0l	300	20	,	,	,
cord-104268-q1jx0n0l	300	21	or	or	CC
cord-104268-q1jx0n0l	300	22	overlap	overlap	VB
cord-104268-q1jx0n0l	300	23	with	with	IN
cord-104268-q1jx0n0l	300	24	,	,	,
cord-104268-q1jx0n0l	300	25	compartments	compartment	NNS
cord-104268-q1jx0n0l	300	26	containing	contain	VBG
cord-104268-q1jx0n0l	300	27	the	the	DT
cord-104268-q1jx0n0l	300	28	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	300	29	type	type	NN
cord-104268-q1jx0n0l	300	30	II	ii	CD
cord-104268-q1jx0n0l	300	31	membrane	membrane	NN
cord-104268-q1jx0n0l	300	32	N	n	CD
cord-104268-q1jx0n0l	300	33	-	-	HYPH
cord-104268-q1jx0n0l	300	34	glycoprotein	glycoprotein	NN
cord-104268-q1jx0n0l	300	35	guanosine	guanosine	NN
cord-104268-q1jx0n0l	300	36	diphosphatase	diphosphatase	NN
cord-104268-q1jx0n0l	300	37	,	,	,
cord-104268-q1jx0n0l	300	38	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	300	39	(	(	-LRB-
cord-104268-q1jx0n0l	300	40	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	300	41	et	et	NNP
cord-104268-q1jx0n0l	300	42	al	al	NNP
cord-104268-q1jx0n0l	300	43	.	.	NNP
cord-104268-q1jx0n0l	300	44	,	,	,
cord-104268-q1jx0n0l	300	45	1989	1989	CD
cord-104268-q1jx0n0l	300	46	(	(	-LRB-
cord-104268-q1jx0n0l	300	47	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	300	48	et	et	NNP
cord-104268-q1jx0n0l	300	49	al	al	NNP
cord-104268-q1jx0n0l	300	50	.	.	NNP
cord-104268-q1jx0n0l	300	51	,	,	,
cord-104268-q1jx0n0l	300	52	,	,	,
cord-104268-q1jx0n0l	300	53	1993	1993	CD
cord-104268-q1jx0n0l	300	54	.	.	.
cord-104268-q1jx0n0l	301	1	Subcellular	subcellular	JJ
cord-104268-q1jx0n0l	301	2	fractionation	fractionation	NN
cord-104268-q1jx0n0l	301	3	experiments	experiment	NNS
cord-104268-q1jx0n0l	301	4	indicated	indicate	VBD
cord-104268-q1jx0n0l	301	5	that	that	IN
cord-104268-q1jx0n0l	301	6	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	301	7	and	and	CC
cord-104268-q1jx0n0l	301	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	301	9	activities	activity	NNS
cord-104268-q1jx0n0l	301	10	cosediment	cosediment	NNP
cord-104268-q1jx0n0l	301	11	(	(	-LRB-
cord-104268-q1jx0n0l	301	12	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	301	13	et	et	NNP
cord-104268-q1jx0n0l	301	14	al	al	NNP
cord-104268-q1jx0n0l	301	15	.	.	NNP
cord-104268-q1jx0n0l	301	16	,	,	,
cord-104268-q1jx0n0l	301	17	1989	1989	CD
cord-104268-q1jx0n0l	301	18	)	)	-RRB-
cord-104268-q1jx0n0l	301	19	,	,	,
cord-104268-q1jx0n0l	301	20	whereas	whereas	IN
cord-104268-q1jx0n0l	301	21	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	301	22	and	and	CC
cord-104268-q1jx0n0l	301	23	the	the	DT
cord-104268-q1jx0n0l	301	24	late	late	JJ
cord-104268-q1jx0n0l	301	25	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	301	26	marker	marker	NN
cord-104268-q1jx0n0l	301	27	,	,	,
cord-104268-q1jx0n0l	301	28	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	301	29	,	,	,
cord-104268-q1jx0n0l	301	30	are	be	VBP
cord-104268-q1jx0n0l	301	31	in	in	IN
cord-104268-q1jx0n0l	301	32	distinct	distinct	JJ
cord-104268-q1jx0n0l	301	33	compartments	compartment	NNS
cord-104268-q1jx0n0l	301	34	(	(	-LRB-
cord-104268-q1jx0n0l	301	35	Bowser	Bowser	NNP
cord-104268-q1jx0n0l	301	36	and	and	CC
cord-104268-q1jx0n0l	301	37	Novick	Novick	NNP
cord-104268-q1jx0n0l	301	38	,	,	,
cord-104268-q1jx0n0l	301	39	1991	1991	CD
cord-104268-q1jx0n0l	301	40	)	)	-RRB-
cord-104268-q1jx0n0l	301	41	.	.	.
cord-104268-q1jx0n0l	302	1	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	302	2	also	also	RB
cord-104268-q1jx0n0l	302	3	cosediments	cosediment	VBZ
cord-104268-q1jx0n0l	302	4	with	with	IN
cord-104268-q1jx0n0l	302	5	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	302	6	and	and	CC
cord-104268-q1jx0n0l	302	7	was	be	VBD
cord-104268-q1jx0n0l	302	8	found	find	VBN
cord-104268-q1jx0n0l	302	9	in	in	IN
cord-104268-q1jx0n0l	302	10	fractions	fraction	NNS
cord-104268-q1jx0n0l	302	11	different	different	JJ
cord-104268-q1jx0n0l	302	12	from	from	IN
cord-104268-q1jx0n0l	302	13	those	those	DT
cord-104268-q1jx0n0l	302	14	containing	contain	VBG
cord-104268-q1jx0n0l	302	15	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	302	16	.	.	.
cord-104268-q1jx0n0l	303	1	These	these	DT
cord-104268-q1jx0n0l	303	2	results	result	NNS
cord-104268-q1jx0n0l	303	3	are	be	VBP
cord-104268-q1jx0n0l	303	4	in	in	IN
cord-104268-q1jx0n0l	303	5	accord	accord	NN
cord-104268-q1jx0n0l	303	6	with	with	IN
cord-104268-q1jx0n0l	303	7	our	-PRON-	PRP$
cord-104268-q1jx0n0l	303	8	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	303	9	data	datum	NNS
cord-104268-q1jx0n0l	303	10	indicating	indicate	VBG
cord-104268-q1jx0n0l	303	11	that	that	IN
cord-104268-q1jx0n0l	303	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	303	13	and	and	CC
cord-104268-q1jx0n0l	303	14	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	303	15	are	be	VBP
cord-104268-q1jx0n0l	303	16	in	in	IN
cord-104268-q1jx0n0l	303	17	the	the	DT
cord-104268-q1jx0n0l	303	18	same	same	JJ
cord-104268-q1jx0n0l	303	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	303	20	compartment	compartment	NN
cord-104268-q1jx0n0l	303	21	.	.	.
cord-104268-q1jx0n0l	304	1	The	the	DT
cord-104268-q1jx0n0l	304	2	apparent	apparent	JJ
cord-104268-q1jx0n0l	304	3	double	double	JJ
cord-104268-q1jx0n0l	304	4	role	role	NN
cord-104268-q1jx0n0l	304	5	of	of	IN
cord-104268-q1jx0n0l	304	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	304	7	in	in	IN
cord-104268-q1jx0n0l	304	8	O	O	NNP
cord-104268-q1jx0n0l	304	9	-	-	HYPH
cord-104268-q1jx0n0l	304	10	and	and	CC
cord-104268-q1jx0n0l	304	11	N	n	NN
cord-104268-q1jx0n0l	304	12	-	-	HYPH
cord-104268-q1jx0n0l	304	13	linked	link	VBN
cord-104268-q1jx0n0l	304	14	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	305	1	Hill	Hill	NNP
cord-104268-q1jx0n0l	305	2	et	et	NNP
cord-104268-q1jx0n0l	305	3	al	al	NNP
cord-104268-q1jx0n0l	305	4	.	.	.
cord-104268-q1jx0n0l	306	1	,	,	,
cord-104268-q1jx0n0l	306	2	1992	1992	CD
cord-104268-q1jx0n0l	306	3	;	;	:
cord-104268-q1jx0n0l	306	4	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	306	5	et	et	NNP
cord-104268-q1jx0n0l	306	6	al	al	NNP
cord-104268-q1jx0n0l	306	7	.	.	.
cord-104268-q1jx0n0l	307	1	,	,	,
cord-104268-q1jx0n0l	307	2	1995b	1995b	CD
cord-104268-q1jx0n0l	307	3	)	)	-RRB-
cord-104268-q1jx0n0l	307	4	is	be	VBZ
cord-104268-q1jx0n0l	307	5	most	most	RBS
cord-104268-q1jx0n0l	307	6	simply	simply	RB
cord-104268-q1jx0n0l	307	7	explained	explain	VBN
cord-104268-q1jx0n0l	307	8	if	if	IN
cord-104268-q1jx0n0l	307	9	both	both	DT
cord-104268-q1jx0n0l	307	10	types	type	NNS
cord-104268-q1jx0n0l	307	11	of	of	IN
cord-104268-q1jx0n0l	307	12	modification	modification	NN
cord-104268-q1jx0n0l	307	13	are	be	VBP
cord-104268-q1jx0n0l	307	14	carried	carry	VBN
cord-104268-q1jx0n0l	307	15	out	out	RP
cord-104268-q1jx0n0l	307	16	in	in	IN
cord-104268-q1jx0n0l	307	17	the	the	DT
cord-104268-q1jx0n0l	307	18	same	same	JJ
cord-104268-q1jx0n0l	307	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	307	20	compartments	compartment	NNS
cord-104268-q1jx0n0l	307	21	.	.	.
cord-104268-q1jx0n0l	308	1	The	the	DT
cord-104268-q1jx0n0l	308	2	observations	observation	NNS
cord-104268-q1jx0n0l	308	3	that	that	WDT
cord-104268-q1jx0n0l	308	4	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	308	5	also	also	RB
cord-104268-q1jx0n0l	308	6	acts	act	VBZ
cord-104268-q1jx0n0l	308	7	on	on	IN
cord-104268-q1jx0n0l	308	8	O	O	NNP
cord-104268-q1jx0n0l	308	9	-	-	HYPH
cord-104268-q1jx0n0l	308	10	and	and	CC
cord-104268-q1jx0n0l	308	11	N	n	NN
cord-104268-q1jx0n0l	308	12	-	-	HYPH
cord-104268-q1jx0n0l	308	13	linked	link	VBN
cord-104268-q1jx0n0l	308	14	chains	chain	NNS
cord-104268-q1jx0n0l	308	15	(	(	-LRB-
cord-104268-q1jx0n0l	308	16	Ballou	Ballou	NNP
cord-104268-q1jx0n0l	308	17	,	,	,
cord-104268-q1jx0n0l	308	18	1990	1990	CD
cord-104268-q1jx0n0l	308	19	;	;	:
cord-104268-q1jx0n0l	308	20	Herscovics	Herscovics	NNP
cord-104268-q1jx0n0l	308	21	and	and	CC
cord-104268-q1jx0n0l	308	22	Orlean	Orlean	NNP
cord-104268-q1jx0n0l	308	23	,	,	,
cord-104268-q1jx0n0l	308	24	1993	1993	CD
cord-104268-q1jx0n0l	308	25	)	)	-RRB-
cord-104268-q1jx0n0l	308	26	,	,	,
cord-104268-q1jx0n0l	308	27	and	and	CC
cord-104268-q1jx0n0l	308	28	that	that	IN
cord-104268-q1jx0n0l	308	29	the	the	DT
cord-104268-q1jx0n0l	308	30	O	O	NNP
cord-104268-q1jx0n0l	308	31	-	-	HYPH
cord-104268-q1jx0n0l	308	32	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	308	33	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	308	34	is	be	VBZ
cord-104268-q1jx0n0l	308	35	itself	-PRON-	PRP
cord-104268-q1jx0n0l	308	36	N	n	CD
cord-104268-q1jx0n0l	308	37	-	-	HYPH
cord-104268-q1jx0n0l	308	38	glycosylated	glycosylated	JJ
cord-104268-q1jx0n0l	308	39	,	,	,
cord-104268-q1jx0n0l	308	40	are	be	VBP
cord-104268-q1jx0n0l	308	41	consistent	consistent	JJ
cord-104268-q1jx0n0l	308	42	with	with	IN
cord-104268-q1jx0n0l	308	43	a	a	DT
cord-104268-q1jx0n0l	308	44	common	common	JJ
cord-104268-q1jx0n0l	308	45	set	set	NN
cord-104268-q1jx0n0l	308	46	of	of	IN
cord-104268-q1jx0n0l	308	47	compartments	compartment	NNS
cord-104268-q1jx0n0l	308	48	for	for	IN
cord-104268-q1jx0n0l	308	49	these	these	DT
cord-104268-q1jx0n0l	308	50	modifications	modification	NNS
cord-104268-q1jx0n0l	308	51	.	.	.
cord-104268-q1jx0n0l	309	1	However	however	RB
cord-104268-q1jx0n0l	309	2	,	,	,
cord-104268-q1jx0n0l	309	3	more	more	RBR
cord-104268-q1jx0n0l	309	4	complex	complex	JJ
cord-104268-q1jx0n0l	309	5	scenarios	scenario	NNS
cord-104268-q1jx0n0l	309	6	could	could	MD
cord-104268-q1jx0n0l	309	7	be	be	VB
cord-104268-q1jx0n0l	309	8	envisaged	envisage	VBN
cord-104268-q1jx0n0l	309	9	with	with	IN
cord-104268-q1jx0n0l	309	10	specific	specific	JJ
cord-104268-q1jx0n0l	309	11	subcompartments	subcompartment	NNS
cord-104268-q1jx0n0l	309	12	for	for	IN
cord-104268-q1jx0n0l	309	13	O	o	NN
cord-104268-q1jx0n0l	309	14	-	-	HYPH
cord-104268-q1jx0n0l	309	15	and	and	CC
cord-104268-q1jx0n0l	309	16	N	n	CD
cord-104268-q1jx0n0l	309	17	-	-	HYPH
cord-104268-q1jx0n0l	309	18	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	309	19	.	.	.
cord-104268-q1jx0n0l	310	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	310	2	is	be	VBZ
cord-104268-q1jx0n0l	310	3	predicted	predict	VBN
cord-104268-q1jx0n0l	310	4	to	to	TO
cord-104268-q1jx0n0l	310	5	act	act	VB
cord-104268-q1jx0n0l	310	6	in	in	IN
cord-104268-q1jx0n0l	310	7	a	a	DT
cord-104268-q1jx0n0l	310	8	functionally	functionally	RB
cord-104268-q1jx0n0l	310	9	and	and	CC
cord-104268-q1jx0n0l	310	10	genetically	genetically	RB
cord-104268-q1jx0n0l	310	11	defined	define	VBN
cord-104268-q1jx0n0l	310	12	compartment	compartment	NN
cord-104268-q1jx0n0l	310	13	where	where	WRB
cord-104268-q1jx0n0l	310	14	various	various	JJ
cord-104268-q1jx0n0l	310	15	cd,2-mannosyl	cd,2-mannosyl	NN
cord-104268-q1jx0n0l	310	16	residues	residue	NNS
cord-104268-q1jx0n0l	310	17	are	be	VBP
cord-104268-q1jx0n0l	310	18	added	add	VBN
cord-104268-q1jx0n0l	310	19	to	to	IN
cord-104268-q1jx0n0l	310	20	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	310	21	chains	chain	NNS
cord-104268-q1jx0n0l	310	22	.	.	.
cord-104268-q1jx0n0l	311	1	This	this	DT
cord-104268-q1jx0n0l	311	2	compartment(s	compartment(s	NNP
cord-104268-q1jx0n0l	311	3	)	)	-RRB-
cord-104268-q1jx0n0l	311	4	is	be	VBZ
cord-104268-q1jx0n0l	311	5	also	also	RB
cord-104268-q1jx0n0l	311	6	likely	likely	JJ
cord-104268-q1jx0n0l	311	7	to	to	TO
cord-104268-q1jx0n0l	311	8	include	include	VB
cord-104268-q1jx0n0l	311	9	other	other	JJ
cord-104268-q1jx0n0l	311	10	~l,2-mannosyltransferases	~l,2-mannosyltransferases	NFP
cord-104268-q1jx0n0l	311	11	responsible	responsible	JJ
cord-104268-q1jx0n0l	311	12	for	for	IN
cord-104268-q1jx0n0l	311	13	N	n	NN
cord-104268-q1jx0n0l	311	14	-	-	HYPH
cord-104268-q1jx0n0l	311	15	linked	link	VBN
cord-104268-q1jx0n0l	311	16	outer	outer	JJ
cord-104268-q1jx0n0l	311	17	chain	chain	NN
cord-104268-q1jx0n0l	311	18	elaboration	elaboration	NN
cord-104268-q1jx0n0l	311	19	(	(	-LRB-
cord-104268-q1jx0n0l	311	20	Fig	Fig	NNP
cord-104268-q1jx0n0l	311	21	.	.	NNP
cord-104268-q1jx0n0l	311	22	2	2	CD
cord-104268-q1jx0n0l	311	23	)	)	-RRB-
cord-104268-q1jx0n0l	311	24	.	.	.
cord-104268-q1jx0n0l	312	1	A	a	DT
cord-104268-q1jx0n0l	312	2	family	family	NN
cord-104268-q1jx0n0l	312	3	of	of	IN
cord-104268-q1jx0n0l	312	4	putative	putative	JJ
cord-104268-q1jx0n0l	312	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	312	6	-	-	HYPH
cord-104268-q1jx0n0l	312	7	like	like	JJ
cord-104268-q1jx0n0l	312	8	mannosyltransferases	mannosyltransferase	NNS
cord-104268-q1jx0n0l	312	9	has	have	VBZ
cord-104268-q1jx0n0l	312	10	been	be	VBN
cord-104268-q1jx0n0l	312	11	identified	identify	VBN
cord-104268-q1jx0n0l	312	12	(	(	-LRB-
cord-104268-q1jx0n0l	312	13	Hill	Hill	NNP
cord-104268-q1jx0n0l	312	14	et	et	NNP
cord-104268-q1jx0n0l	312	15	al	al	NNP
cord-104268-q1jx0n0l	312	16	.	.	.
cord-104268-q1jx0n0l	313	1	,	,	,
cord-104268-q1jx0n0l	313	2	1992	1992	CD
cord-104268-q1jx0n0l	313	3	;	;	:
cord-104268-q1jx0n0l	313	4	Bussereau	Bussereau	NNP
cord-104268-q1jx0n0l	313	5	et	et	NNP
cord-104268-q1jx0n0l	313	6	al	al	NNP
cord-104268-q1jx0n0l	313	7	.	.	.
cord-104268-q1jx0n0l	314	1	,	,	,
cord-104268-q1jx0n0l	314	2	1993	1993	CD
cord-104268-q1jx0n0l	314	3	;	;	:
cord-104268-q1jx0n0l	314	4	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	314	5	et	et	NNP
cord-104268-q1jx0n0l	314	6	al	al	NNP
cord-104268-q1jx0n0l	314	7	.	.	NNP
cord-104268-q1jx0n0l	314	8	,	,	,
cord-104268-q1jx0n0l	314	9	1993	1993	CD
cord-104268-q1jx0n0l	314	10	;	;	:
cord-104268-q1jx0n0l	314	11	Mallet	Mallet	NNP
cord-104268-q1jx0n0l	314	12	et	et	NNP
cord-104268-q1jx0n0l	314	13	al	al	NNP
cord-104268-q1jx0n0l	314	14	.	.	NNP
cord-104268-q1jx0n0l	314	15	,	,	,
cord-104268-q1jx0n0l	314	16	1994	1994	CD
cord-104268-q1jx0n0l	314	17	)	)	-RRB-
cord-104268-q1jx0n0l	314	18	,	,	,
cord-104268-q1jx0n0l	314	19	and	and	CC
cord-104268-q1jx0n0l	314	20	some	some	DT
cord-104268-q1jx0n0l	314	21	members	member	NNS
cord-104268-q1jx0n0l	314	22	have	have	VBP
cord-104268-q1jx0n0l	314	23	been	be	VBN
cord-104268-q1jx0n0l	314	24	shown	show	VBN
cord-104268-q1jx0n0l	314	25	bioehemically	bioehemically	RB
cord-104268-q1jx0n0l	314	26	to	to	TO
cord-104268-q1jx0n0l	314	27	be	be	VB
cord-104268-q1jx0n0l	314	28	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	314	29	mannosyltransferases	mannosyltransferase	NNS
cord-104268-q1jx0n0l	314	30	(	(	-LRB-
cord-104268-q1jx0n0l	314	31	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	314	32	et	et	NNP
cord-104268-q1jx0n0l	314	33	al	al	NNP
cord-104268-q1jx0n0l	314	34	.	.	NNP
cord-104268-q1jx0n0l	314	35	,	,	,
cord-104268-q1jx0n0l	314	36	1995b	1995b	CD
cord-104268-q1jx0n0l	314	37	)	)	-RRB-
cord-104268-q1jx0n0l	314	38	.	.	.
cord-104268-q1jx0n0l	315	1	Immunolocalization	immunolocalization	NN
cord-104268-q1jx0n0l	315	2	results	result	VBZ
cord-104268-q1jx0n0l	315	3	from	from	IN
cord-104268-q1jx0n0l	315	4	the	the	DT
cord-104268-q1jx0n0l	315	5	different	different	JJ
cord-104268-q1jx0n0l	315	6	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	315	7	proteins	protein	NNS
cord-104268-q1jx0n0l	315	8	demonstrate	demonstrate	VBP
cord-104268-q1jx0n0l	315	9	that	that	IN
cord-104268-q1jx0n0l	315	10	the	the	DT
cord-104268-q1jx0n0l	315	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	315	12	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	315	13	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	315	14	domain	domain	NN
cord-104268-q1jx0n0l	315	15	was	be	VBD
cord-104268-q1jx0n0l	315	16	required	require	VBN
cord-104268-q1jx0n0l	315	17	for	for	IN
cord-104268-q1jx0n0l	315	18	localization	localization	NN
cord-104268-q1jx0n0l	315	19	to	to	IN
cord-104268-q1jx0n0l	315	20	the	the	DT
cord-104268-q1jx0n0l	315	21	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	315	22	complex	complex	NN
cord-104268-q1jx0n0l	315	23	.	.	.
cord-104268-q1jx0n0l	316	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	316	2	proteins	protein	NNS
cord-104268-q1jx0n0l	316	3	lacking	lack	VBG
cord-104268-q1jx0n0l	316	4	the	the	DT
cord-104268-q1jx0n0l	316	5	11-amino	11-amino	CD
cord-104268-q1jx0n0l	316	6	acid	acid	NN
cord-104268-q1jx0n0l	316	7	residue	residue	NN
cord-104268-q1jx0n0l	316	8	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	316	9	domain	domain	NN
cord-104268-q1jx0n0l	316	10	are	be	VBP
cord-104268-q1jx0n0l	316	11	fully	fully	RB
cord-104268-q1jx0n0l	316	12	active	active	JJ
cord-104268-q1jx0n0l	316	13	as	as	IN
cord-104268-q1jx0n0l	316	14	a	a	DT
cord-104268-q1jx0n0l	316	15	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	316	16	in	in	IN
cord-104268-q1jx0n0l	316	17	vitro	vitro	FW
cord-104268-q1jx0n0l	316	18	but	but	CC
cord-104268-q1jx0n0l	316	19	fail	fail	VB
cord-104268-q1jx0n0l	316	20	to	to	TO
cord-104268-q1jx0n0l	316	21	be	be	VB
cord-104268-q1jx0n0l	316	22	properly	properly	RB
cord-104268-q1jx0n0l	316	23	retained	retain	VBN
cord-104268-q1jx0n0l	316	24	and	and	CC
cord-104268-q1jx0n0l	316	25	are	be	VBP
cord-104268-q1jx0n0l	316	26	mislocalized	mislocalize	VBN
cord-104268-q1jx0n0l	316	27	to	to	IN
cord-104268-q1jx0n0l	316	28	the	the	DT
cord-104268-q1jx0n0l	316	29	vacuole	vacuole	NN
cord-104268-q1jx0n0l	316	30	.	.	.
cord-104268-q1jx0n0l	317	1	A	a	DT
cord-104268-q1jx0n0l	317	2	trivial	trivial	JJ
cord-104268-q1jx0n0l	317	3	explanation	explanation	NN
cord-104268-q1jx0n0l	317	4	for	for	IN
cord-104268-q1jx0n0l	317	5	the	the	DT
cord-104268-q1jx0n0l	317	6	localization	localization	NN
cord-104268-q1jx0n0l	317	7	of	of	IN
cord-104268-q1jx0n0l	317	8	D	d	NN
cord-104268-q1jx0n0l	317	9	K	k	NN
cord-104268-q1jx0n0l	317	10	K	k	NN
cord-104268-q1jx0n0l	317	11	K	K	NNP
cord-104268-q1jx0n0l	317	12	to	to	IN
cord-104268-q1jx0n0l	317	13	the	the	DT
cord-104268-q1jx0n0l	317	14	vacuole	vacuole	NN
cord-104268-q1jx0n0l	317	15	is	be	VBZ
cord-104268-q1jx0n0l	317	16	that	that	IN
cord-104268-q1jx0n0l	317	17	the	the	DT
cord-104268-q1jx0n0l	317	18	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	317	19	domain	domain	NN
cord-104268-q1jx0n0l	317	20	of	of	IN
cord-104268-q1jx0n0l	317	21	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	317	22	B	B	NNP
cord-104268-q1jx0n0l	317	23	contains	contain	VBZ
cord-104268-q1jx0n0l	317	24	a	a	DT
cord-104268-q1jx0n0l	317	25	cryptic	cryptic	JJ
cord-104268-q1jx0n0l	317	26	vacuolar	vacuolar	NN
cord-104268-q1jx0n0l	317	27	targeting	targeting	NN
cord-104268-q1jx0n0l	317	28	signal	signal	NN
cord-104268-q1jx0n0l	317	29	that	that	WDT
cord-104268-q1jx0n0l	317	30	overrides	override	VBZ
cord-104268-q1jx0n0l	317	31	the	the	DT
cord-104268-q1jx0n0l	317	32	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	317	33	-	-	HYPH
cord-104268-q1jx0n0l	317	34	targeting	target	VBG
cord-104268-q1jx0n0l	317	35	sequence	sequence	NN
cord-104268-q1jx0n0l	317	36	of	of	IN
cord-104268-q1jx0n0l	317	37	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	317	38	.	.	.
cord-104268-q1jx0n0l	318	1	Arguing	argue	VBG
cord-104268-q1jx0n0l	318	2	against	against	IN
cord-104268-q1jx0n0l	318	3	this	this	DT
cord-104268-q1jx0n0l	318	4	view	view	NN
cord-104268-q1jx0n0l	318	5	is	be	VBZ
cord-104268-q1jx0n0l	318	6	the	the	DT
cord-104268-q1jx0n0l	318	7	demonstration	demonstration	NN
cord-104268-q1jx0n0l	318	8	that	that	WDT
cord-104268-q1jx0n0l	318	9	the	the	DT
cord-104268-q1jx0n0l	318	10	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	318	11	domain	domain	NN
cord-104268-q1jx0n0l	318	12	of	of	IN
cord-104268-q1jx0n0l	318	13	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	318	14	B	B	NNP
cord-104268-q1jx0n0l	318	15	is	be	VBZ
cord-104268-q1jx0n0l	318	16	not	not	RB
cord-104268-q1jx0n0l	318	17	necessary	necessary	JJ
cord-104268-q1jx0n0l	318	18	for	for	IN
cord-104268-q1jx0n0l	318	19	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	318	20	targeting	target	VBG
cord-104268-q1jx0n0l	318	21	(	(	-LRB-
cord-104268-q1jx0n0l	318	22	Roberts	Roberts	NNP
cord-104268-q1jx0n0l	318	23	et	et	NNP
cord-104268-q1jx0n0l	318	24	al	al	NNP
cord-104268-q1jx0n0l	318	25	.	.	NNP
cord-104268-q1jx0n0l	318	26	,	,	,
cord-104268-q1jx0n0l	318	27	1992	1992	CD
cord-104268-q1jx0n0l	318	28	;	;	:
cord-104268-q1jx0n0l	318	29	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	318	30	et	et	NNP
cord-104268-q1jx0n0l	318	31	al	al	NNP
cord-104268-q1jx0n0l	318	32	.	.	NNP
cord-104268-q1jx0n0l	318	33	,	,	,
cord-104268-q1jx0n0l	318	34	1993	1993	CD
cord-104268-q1jx0n0l	318	35	)	)	-RRB-
cord-104268-q1jx0n0l	318	36	.	.	.
cord-104268-q1jx0n0l	319	1	To	to	TO
cord-104268-q1jx0n0l	319	2	ensure	ensure	VB
cord-104268-q1jx0n0l	319	3	that	that	IN
cord-104268-q1jx0n0l	319	4	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	319	5	of	of	IN
cord-104268-q1jx0n0l	319	6	DKKK	DKKK	NNP
cord-104268-q1jx0n0l	319	7	was	be	VBD
cord-104268-q1jx0n0l	319	8	not	not	RB
cord-104268-q1jx0n0l	319	9	caused	cause	VBN
cord-104268-q1jx0n0l	319	10	by	by	IN
cord-104268-q1jx0n0l	319	11	some	some	DT
cord-104268-q1jx0n0l	319	12	previously	previously	RB
cord-104268-q1jx0n0l	319	13	unrecognized	unrecognized	JJ
cord-104268-q1jx0n0l	319	14	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	319	15	targeting	target	VBG
cord-104268-q1jx0n0l	319	16	signal	signal	NN
cord-104268-q1jx0n0l	319	17	of	of	IN
cord-104268-q1jx0n0l	319	18	the	the	DT
cord-104268-q1jx0n0l	319	19	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	319	20	domain	domain	NN
cord-104268-q1jx0n0l	319	21	of	of	IN
cord-104268-q1jx0n0l	319	22	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	319	23	B	B	NNP
cord-104268-q1jx0n0l	319	24	,	,	,
cord-104268-q1jx0n0l	319	25	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	319	26	protein	protein	NN
cord-104268-q1jx0n0l	319	27	MR	MR	NNP
cord-104268-q1jx0n0l	319	28	/	/	SYM
cord-104268-q1jx0n0l	319	29	KKK	KKK	NNP
cord-104268-q1jx0n0l	319	30	was	be	VBD
cord-104268-q1jx0n0l	319	31	constructed	construct	VBN
cord-104268-q1jx0n0l	319	32	in	in	IN
cord-104268-q1jx0n0l	319	33	which	which	WDT
cord-104268-q1jx0n0l	319	34	the	the	DT
cord-104268-q1jx0n0l	319	35	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	319	36	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	319	37	tail	tail	NN
cord-104268-q1jx0n0l	319	38	was	be	VBD
cord-104268-q1jx0n0l	319	39	deleted	delete	VBN
cord-104268-q1jx0n0l	319	40	.	.	.
cord-104268-q1jx0n0l	320	1	As	as	IN
cord-104268-q1jx0n0l	320	2	with	with	IN
cord-104268-q1jx0n0l	320	3	DKKK	DKKK	NNP
cord-104268-q1jx0n0l	320	4	,	,	,
cord-104268-q1jx0n0l	320	5	MR/	MR/	NNP
cord-104268-q1jx0n0l	320	6	KKK	KKK	NNP
cord-104268-q1jx0n0l	320	7	was	be	VBD
cord-104268-q1jx0n0l	320	8	mislocalized	mislocalize	VBN
cord-104268-q1jx0n0l	320	9	to	to	IN
cord-104268-q1jx0n0l	320	10	the	the	DT
cord-104268-q1jx0n0l	320	11	vacuole	vacuole	NN
cord-104268-q1jx0n0l	320	12	,	,	,
cord-104268-q1jx0n0l	320	13	showing	show	VBG
cord-104268-q1jx0n0l	320	14	that	that	IN
cord-104268-q1jx0n0l	320	15	the	the	DT
cord-104268-q1jx0n0l	320	16	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	320	17	domain	domain	NN
cord-104268-q1jx0n0l	320	18	of	of	IN
cord-104268-q1jx0n0l	320	19	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	320	20	is	be	VBZ
cord-104268-q1jx0n0l	320	21	necessary	necessary	JJ
cord-104268-q1jx0n0l	320	22	for	for	IN
cord-104268-q1jx0n0l	320	23	proper	proper	JJ
cord-104268-q1jx0n0l	320	24	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	320	25	targeting	targeting	NN
cord-104268-q1jx0n0l	320	26	.	.	.
cord-104268-q1jx0n0l	321	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	321	2	proteins	protein	NNS
cord-104268-q1jx0n0l	322	1	K	k	NN
cord-104268-q1jx0n0l	322	2	D	d	NN
cord-104268-q1jx0n0l	322	3	K	k	NN
cord-104268-q1jx0n0l	322	4	K	K	NNP
cord-104268-q1jx0n0l	322	5	and	and	CC
cord-104268-q1jx0n0l	322	6	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	322	7	could	could	MD
cord-104268-q1jx0n0l	322	8	not	not	RB
cord-104268-q1jx0n0l	322	9	be	be	VB
cord-104268-q1jx0n0l	322	10	distinguished	distinguish	VBN
cord-104268-q1jx0n0l	322	11	from	from	IN
cord-104268-q1jx0n0l	322	12	wild	wild	JJ
cord-104268-q1jx0n0l	322	13	-	-	HYPH
cord-104268-q1jx0n0l	322	14	type	type	NN
cord-104268-q1jx0n0l	322	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	322	16	,	,	,
cord-104268-q1jx0n0l	322	17	permitting	permit	VBG
cord-104268-q1jx0n0l	322	18	the	the	DT
cord-104268-q1jx0n0l	322	19	conclusion	conclusion	NN
cord-104268-q1jx0n0l	322	20	that	that	IN
cord-104268-q1jx0n0l	322	21	the	the	DT
cord-104268-q1jx0n0l	322	22	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	322	23	TMD	TMD	NNP
cord-104268-q1jx0n0l	322	24	in	in	IN
cord-104268-q1jx0n0l	322	25	the	the	DT
cord-104268-q1jx0n0l	322	26	context	context	NN
cord-104268-q1jx0n0l	322	27	of	of	IN
cord-104268-q1jx0n0l	322	28	a	a	DT
cord-104268-q1jx0n0l	322	29	native	native	JJ
cord-104268-q1jx0n0l	322	30	protein	protein	NN
cord-104268-q1jx0n0l	322	31	is	be	VBZ
cord-104268-q1jx0n0l	322	32	not	not	RB
cord-104268-q1jx0n0l	322	33	necessary	necessary	JJ
cord-104268-q1jx0n0l	322	34	for	for	IN
cord-104268-q1jx0n0l	322	35	correct	correct	JJ
cord-104268-q1jx0n0l	322	36	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	322	37	localization	localization	NN
cord-104268-q1jx0n0l	322	38	.	.	.
cord-104268-q1jx0n0l	323	1	Chimeric	chimeric	JJ
cord-104268-q1jx0n0l	323	2	construct	construct	VBP
cord-104268-q1jx0n0l	323	3	KD	KD	NNP
cord-104268-q1jx0n0l	323	4	-	-	HYPH
cord-104268-q1jx0n0l	323	5	K	K	NNP
cord-104268-q1jx0n0l	323	6	which	which	WDT
cord-104268-q1jx0n0l	323	7	comprises	comprise	VBZ
cord-104268-q1jx0n0l	323	8	the	the	DT
cord-104268-q1jx0n0l	323	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	323	10	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	323	11	tail	tail	NN
cord-104268-q1jx0n0l	323	12	,	,	,
cord-104268-q1jx0n0l	323	13	the	the	DT
cord-104268-q1jx0n0l	323	14	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	323	15	B	B	NNP
cord-104268-q1jx0n0l	323	16	membrane	membrane	NN
cord-104268-q1jx0n0l	323	17	-	-	HYPH
cord-104268-q1jx0n0l	323	18	spanning	span	VBG
cord-104268-q1jx0n0l	323	19	domain	domain	NN
cord-104268-q1jx0n0l	323	20	,	,	,
cord-104268-q1jx0n0l	323	21	a	a	DT
cord-104268-q1jx0n0l	323	22	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	323	23	partial	partial	JJ
cord-104268-q1jx0n0l	323	24	stem	stem	NN
cord-104268-q1jx0n0l	323	25	region	region	NN
cord-104268-q1jx0n0l	323	26	,	,	,
cord-104268-q1jx0n0l	323	27	and	and	CC
cord-104268-q1jx0n0l	323	28	the	the	DT
cord-104268-q1jx0n0l	323	29	Kre2	Kre2	NNP
cord-104268-q1jx0n0l	323	30	protein	protein	NN
cord-104268-q1jx0n0l	323	31	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	323	32	domain	domain	NN
cord-104268-q1jx0n0l	323	33	was	be	VBD
cord-104268-q1jx0n0l	323	34	correctly	correctly	RB
cord-104268-q1jx0n0l	323	35	localized	localize	VBN
cord-104268-q1jx0n0l	323	36	to	to	IN
cord-104268-q1jx0n0l	323	37	the	the	DT
cord-104268-q1jx0n0l	323	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	323	39	complex	complex	NN
cord-104268-q1jx0n0l	323	40	.	.	.
cord-104268-q1jx0n0l	324	1	This	this	DT
cord-104268-q1jx0n0l	324	2	indicates	indicate	VBZ
cord-104268-q1jx0n0l	324	3	that	that	IN
cord-104268-q1jx0n0l	324	4	the	the	DT
cord-104268-q1jx0n0l	324	5	first	first	JJ
cord-104268-q1jx0n0l	324	6	36	36	CD
cord-104268-q1jx0n0l	324	7	amino	amino	NN
cord-104268-q1jx0n0l	324	8	acid	acid	NN
cord-104268-q1jx0n0l	324	9	residues	residue	NNS
cord-104268-q1jx0n0l	324	10	of	of	IN
cord-104268-q1jx0n0l	324	11	the	the	DT
cord-104268-q1jx0n0l	324	12	stem	stem	NN
cord-104268-q1jx0n0l	324	13	region	region	NN
cord-104268-q1jx0n0l	324	14	of	of	IN
cord-104268-q1jx0n0l	324	15	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	324	16	are	be	VBP
cord-104268-q1jx0n0l	324	17	dispensable	dispensable	JJ
cord-104268-q1jx0n0l	324	18	for	for	IN
cord-104268-q1jx0n0l	324	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	324	20	targeting	targeting	NN
cord-104268-q1jx0n0l	324	21	.	.	.
cord-104268-q1jx0n0l	325	1	The	the	DT
cord-104268-q1jx0n0l	325	2	results	result	NNS
cord-104268-q1jx0n0l	325	3	obtained	obtain	VBN
cord-104268-q1jx0n0l	325	4	with	with	IN
cord-104268-q1jx0n0l	325	5	this	this	DT
cord-104268-q1jx0n0l	325	6	fusion	fusion	NN
cord-104268-q1jx0n0l	325	7	protein	protein	NN
cord-104268-q1jx0n0l	325	8	again	again	RB
cord-104268-q1jx0n0l	325	9	show	show	VBP
cord-104268-q1jx0n0l	325	10	that	that	IN
cord-104268-q1jx0n0l	325	11	the	the	DT
cord-104268-q1jx0n0l	325	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	325	13	TMD	TMD	NNP
cord-104268-q1jx0n0l	325	14	is	be	VBZ
cord-104268-q1jx0n0l	325	15	not	not	RB
cord-104268-q1jx0n0l	325	16	required	require	VBN
cord-104268-q1jx0n0l	325	17	for	for	IN
cord-104268-q1jx0n0l	325	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	325	19	retention	retention	NN
cord-104268-q1jx0n0l	325	20	.	.	.
cord-104268-q1jx0n0l	326	1	The	the	DT
cord-104268-q1jx0n0l	326	2	fact	fact	NN
cord-104268-q1jx0n0l	326	3	that	that	IN
cord-104268-q1jx0n0l	326	4	in	in	IN
cord-104268-q1jx0n0l	326	5	the	the	DT
cord-104268-q1jx0n0l	326	6	KD	KD	NNP
cord-104268-q1jx0n0l	326	7	-	-	HYPH
cord-104268-q1jx0n0l	326	8	K	K	NNP
cord-104268-q1jx0n0l	326	9	construct	construct	NN
cord-104268-q1jx0n0l	326	10	,	,	,
cord-104268-q1jx0n0l	326	11	the	the	DT
cord-104268-q1jx0n0l	326	12	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	326	13	TMD	TMD	NNP
cord-104268-q1jx0n0l	326	14	and	and	CC
cord-104268-q1jx0n0l	326	15	part	part	NN
cord-104268-q1jx0n0l	326	16	of	of	IN
cord-104268-q1jx0n0l	326	17	the	the	DT
cord-104268-q1jx0n0l	326	18	stem	stem	NN
cord-104268-q1jx0n0l	326	19	region	region	NN
cord-104268-q1jx0n0l	326	20	are	be	VBP
cord-104268-q1jx0n0l	326	21	not	not	RB
cord-104268-q1jx0n0l	326	22	present	present	JJ
cord-104268-q1jx0n0l	326	23	,	,	,
cord-104268-q1jx0n0l	326	24	argues	argue	VBZ
cord-104268-q1jx0n0l	326	25	that	that	IN
cord-104268-q1jx0n0l	326	26	in	in	IN
cord-104268-q1jx0n0l	326	27	the	the	DT
cord-104268-q1jx0n0l	326	28	context	context	NN
cord-104268-q1jx0n0l	326	29	of	of	IN
cord-104268-q1jx0n0l	326	30	a	a	DT
cord-104268-q1jx0n0l	326	31	protein	protein	NN
cord-104268-q1jx0n0l	326	32	containing	contain	VBG
cord-104268-q1jx0n0l	326	33	a	a	DT
cord-104268-q1jx0n0l	326	34	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	326	35	luminal	luminal	JJ
cord-104268-q1jx0n0l	326	36	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	326	37	domain	domain	NN
cord-104268-q1jx0n0l	326	38	the	the	DT
cord-104268-q1jx0n0l	326	39	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	326	40	tail	tail	NN
cord-104268-q1jx0n0l	326	41	of	of	IN
cord-104268-q1jx0n0l	326	42	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	326	43	is	be	VBZ
cord-104268-q1jx0n0l	326	44	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	326	45	to	to	TO
cord-104268-q1jx0n0l	326	46	correctly	correctly	RB
cord-104268-q1jx0n0l	326	47	target	target	VB
cord-104268-q1jx0n0l	326	48	such	such	PDT
cord-104268-q1jx0n0l	326	49	a	a	DT
cord-104268-q1jx0n0l	326	50	chimera	chimera	NN
cord-104268-q1jx0n0l	326	51	to	to	IN
cord-104268-q1jx0n0l	326	52	the	the	DT
cord-104268-q1jx0n0l	326	53	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	326	54	complex	complex	NN
cord-104268-q1jx0n0l	326	55	,	,	,
cord-104268-q1jx0n0l	326	56	un-	un-	JJ
cord-104268-q1jx0n0l	326	57	Figure	figure	NN
cord-104268-q1jx0n0l	326	58	I0	i0	NN
cord-104268-q1jx0n0l	326	59	.	.	.
cord-104268-q1jx0n0l	327	1	Localization	localization	NN
cord-104268-q1jx0n0l	327	2	of	of	IN
cord-104268-q1jx0n0l	327	3	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	327	4	proteins	protein	NNS
cord-104268-q1jx0n0l	327	5	KKKP	KKKP	NNS
cord-104268-q1jx0n0l	327	6	and	and	CC
cord-104268-q1jx0n0l	327	7	KKP	KKP	NNP
cord-104268-q1jx0n0l	327	8	.	.	.
cord-104268-q1jx0n0l	328	1	Yeast	yeast	NN
cord-104268-q1jx0n0l	328	2	cells	cell	NNS
cord-104268-q1jx0n0l	328	3	carrying	carry	VBG
cord-104268-q1jx0n0l	328	4	a	a	DT
cord-104268-q1jx0n0l	328	5	PH08	PH08	NNP
cord-104268-q1jx0n0l	328	6	disruption	disruption	NN
cord-104268-q1jx0n0l	328	7	(	(	-LRB-
cord-104268-q1jx0n0l	328	8	pho8::TRP1	pho8::trp1	RB
cord-104268-q1jx0n0l	328	9	)	)	-RRB-
cord-104268-q1jx0n0l	328	10	in	in	IN
cord-104268-q1jx0n0l	328	11	a	a	DT
cord-104268-q1jx0n0l	328	12	SEY6210	SEY6210	NNP
cord-104268-q1jx0n0l	328	13	background	background	NN
cord-104268-q1jx0n0l	328	14	and	and	CC
cord-104268-q1jx0n0l	328	15	containing	contain	VBG
cord-104268-q1jx0n0l	328	16	the	the	DT
cord-104268-q1jx0n0l	328	17	different	different	JJ
cord-104268-q1jx0n0l	328	18	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	328	19	constructions	construction	NNS
cord-104268-q1jx0n0l	328	20	on	on	IN
cord-104268-q1jx0n0l	328	21	YEp352	YEp352	NNS
cord-104268-q1jx0n0l	328	22	were	be	VBD
cord-104268-q1jx0n0l	328	23	fixed	fix	VBN
cord-104268-q1jx0n0l	328	24	,	,	,
cord-104268-q1jx0n0l	328	25	spheroplasted	spheroplaste	VBN
cord-104268-q1jx0n0l	328	26	,	,	,
cord-104268-q1jx0n0l	328	27	attached	attach	VBN
cord-104268-q1jx0n0l	328	28	to	to	IN
cord-104268-q1jx0n0l	328	29	polylysine	polylysine	NN
cord-104268-q1jx0n0l	328	30	-	-	HYPH
cord-104268-q1jx0n0l	328	31	coated	coat	VBN
cord-104268-q1jx0n0l	328	32	glass	glass	NN
cord-104268-q1jx0n0l	328	33	slides	slide	NNS
cord-104268-q1jx0n0l	328	34	,	,	,
cord-104268-q1jx0n0l	328	35	and	and	CC
cord-104268-q1jx0n0l	328	36	incubated	incubate	VBN
cord-104268-q1jx0n0l	328	37	with	with	IN
cord-104268-q1jx0n0l	328	38	anti	anti	JJ
cord-104268-q1jx0n0l	328	39	-	-	JJ
cord-104268-q1jx0n0l	328	40	Pho8p	pho8p	JJ
cord-104268-q1jx0n0l	328	41	antibodies	antibody	NNS
cord-104268-q1jx0n0l	328	42	followed	follow	VBD
cord-104268-q1jx0n0l	328	43	by	by	IN
cord-104268-q1jx0n0l	328	44	a	a	DT
cord-104268-q1jx0n0l	328	45	subsequent	subsequent	JJ
cord-104268-q1jx0n0l	328	46	incubation	incubation	NN
cord-104268-q1jx0n0l	328	47	with	with	IN
cord-104268-q1jx0n0l	328	48	a	a	DT
cord-104268-q1jx0n0l	328	49	FITCcoupled	fitccoupled	JJ
cord-104268-q1jx0n0l	328	50	anti	anti	JJ
cord-104268-q1jx0n0l	328	51	-	-	JJ
cord-104268-q1jx0n0l	328	52	rabbit	rabbit	JJ
cord-104268-q1jx0n0l	328	53	secondary	secondary	JJ
cord-104268-q1jx0n0l	328	54	Ab	ab	NN
cord-104268-q1jx0n0l	328	55	and	and	CC
cord-104268-q1jx0n0l	328	56	DAPI	DAPI	NNP
cord-104268-q1jx0n0l	328	57	.	.	.
cord-104268-q1jx0n0l	329	1	Colocalization	Colocalization	NNP
cord-104268-q1jx0n0l	329	2	of	of	IN
cord-104268-q1jx0n0l	329	3	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	329	4	localized	localize	VBN
cord-104268-q1jx0n0l	329	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	329	6	proteins	protein	NNS
cord-104268-q1jx0n0l	329	7	with	with	IN
cord-104268-q1jx0n0l	329	8	the	the	DT
cord-104268-q1jx0n0l	329	9	yeast	yeast	NN
cord-104268-q1jx0n0l	329	10	vacuolar	vacuolar	NN
cord-104268-q1jx0n0l	329	11	membrane	membrane	NN
cord-104268-q1jx0n0l	329	12	H+-ATPase	H+-ATPase	NNP
cord-104268-q1jx0n0l	329	13	was	be	VBD
cord-104268-q1jx0n0l	329	14	achieved	achieve	VBN
cord-104268-q1jx0n0l	329	15	by	by	IN
cord-104268-q1jx0n0l	329	16	coincubation	coincubation	NN
cord-104268-q1jx0n0l	329	17	of	of	IN
cord-104268-q1jx0n0l	329	18	fixed	fix	VBN
cord-104268-q1jx0n0l	329	19	cells	cell	NNS
cord-104268-q1jx0n0l	329	20	along	along	IN
cord-104268-q1jx0n0l	329	21	with	with	IN
cord-104268-q1jx0n0l	329	22	mAb	mab	NN
cord-104268-q1jx0n0l	329	23	13Dll	13dll	CD
cord-104268-q1jx0n0l	329	24	(	(	-LRB-
cord-104268-q1jx0n0l	329	25	Kane	Kane	NNP
cord-104268-q1jx0n0l	329	26	et	et	NNP
cord-104268-q1jx0n0l	329	27	al	al	NNP
cord-104268-q1jx0n0l	329	28	.	.	NNP
cord-104268-q1jx0n0l	329	29	,	,	,
cord-104268-q1jx0n0l	329	30	1992	1992	CD
cord-104268-q1jx0n0l	329	31	)	)	-RRB-
cord-104268-q1jx0n0l	329	32	followed	follow	VBN
cord-104268-q1jx0n0l	329	33	by	by	IN
cord-104268-q1jx0n0l	329	34	incubation	incubation	NN
cord-104268-q1jx0n0l	329	35	of	of	IN
cord-104268-q1jx0n0l	329	36	treated	treat	VBN
cord-104268-q1jx0n0l	329	37	cells	cell	NNS
cord-104268-q1jx0n0l	329	38	with	with	IN
cord-104268-q1jx0n0l	329	39	Texas	Texas	NNP
cord-104268-q1jx0n0l	329	40	red	red	JJ
cord-104268-q1jx0n0l	329	41	-	-	HYPH
cord-104268-q1jx0n0l	329	42	conjugated	conjugate	VBN
cord-104268-q1jx0n0l	329	43	anti	anti	JJ
cord-104268-q1jx0n0l	329	44	-	-	JJ
cord-104268-q1jx0n0l	329	45	mouse	mouse	JJ
cord-104268-q1jx0n0l	329	46	secondary	secondary	JJ
cord-104268-q1jx0n0l	329	47	antibodies	antibody	NNS
cord-104268-q1jx0n0l	329	48	.	.	.
cord-104268-q1jx0n0l	330	1	Arrows	arrow	NNS
cord-104268-q1jx0n0l	330	2	point	point	VBP
cord-104268-q1jx0n0l	330	3	to	to	IN
cord-104268-q1jx0n0l	330	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	330	5	punctiform	punctiform	NN
cord-104268-q1jx0n0l	330	6	structures	structure	NNS
cord-104268-q1jx0n0l	330	7	.	.	.
cord-104268-q1jx0n0l	331	1	less	less	RBR
cord-104268-q1jx0n0l	331	2	some	some	DT
cord-104268-q1jx0n0l	331	3	remaining	remain	VBG
cord-104268-q1jx0n0l	331	4	luminal	luminal	JJ
cord-104268-q1jx0n0l	331	5	sequence	sequence	NN
cord-104268-q1jx0n0l	331	6	also	also	RB
cord-104268-q1jx0n0l	331	7	plays	play	VBZ
cord-104268-q1jx0n0l	331	8	some	some	DT
cord-104268-q1jx0n0l	331	9	role	role	NN
cord-104268-q1jx0n0l	331	10	in	in	IN
cord-104268-q1jx0n0l	331	11	retention	retention	NN
cord-104268-q1jx0n0l	331	12	.	.	.
cord-104268-q1jx0n0l	332	1	To	to	TO
cord-104268-q1jx0n0l	332	2	analyze	analyze	VB
cord-104268-q1jx0n0l	332	3	further	further	RB
cord-104268-q1jx0n0l	332	4	the	the	DT
cord-104268-q1jx0n0l	332	5	targeting	targeting	JJ
cord-104268-q1jx0n0l	332	6	role	role	NN
cord-104268-q1jx0n0l	332	7	of	of	IN
cord-104268-q1jx0n0l	332	8	the	the	DT
cord-104268-q1jx0n0l	332	9	three	three	CD
cord-104268-q1jx0n0l	332	10	nonenzymatic	nonenzymatic	JJ
cord-104268-q1jx0n0l	332	11	domains	domain	NNS
cord-104268-q1jx0n0l	332	12	of	of	IN
cord-104268-q1jx0n0l	332	13	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	332	14	and	and	CC
cord-104268-q1jx0n0l	332	15	to	to	TO
cord-104268-q1jx0n0l	332	16	remove	remove	VB
cord-104268-q1jx0n0l	332	17	the	the	DT
cord-104268-q1jx0n0l	332	18	possible	possible	JJ
cord-104268-q1jx0n0l	332	19	additional	additional	JJ
cord-104268-q1jx0n0l	332	20	complexity	complexity	NN
cord-104268-q1jx0n0l	332	21	of	of	IN
cord-104268-q1jx0n0l	332	22	a	a	DT
cord-104268-q1jx0n0l	332	23	targeting	targeting	NN
cord-104268-q1jx0n0l	332	24	mechanism	mechanism	NN
cord-104268-q1jx0n0l	332	25	involving	involve	VBG
cord-104268-q1jx0n0l	332	26	the	the	DT
cord-104268-q1jx0n0l	332	27	mannosyltransferase	mannosyltransferase	JJ
cord-104268-q1jx0n0l	332	28	portion	portion	NN
cord-104268-q1jx0n0l	332	29	of	of	IN
cord-104268-q1jx0n0l	332	30	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	332	31	,	,	,
cord-104268-q1jx0n0l	332	32	combinations	combination	NNS
cord-104268-q1jx0n0l	332	33	of	of	IN
cord-104268-q1jx0n0l	332	34	noncatalytic	noncatalytic	JJ
cord-104268-q1jx0n0l	332	35	domains	domain	NNS
cord-104268-q1jx0n0l	332	36	of	of	IN
cord-104268-q1jx0n0l	332	37	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	332	38	were	be	VBD
cord-104268-q1jx0n0l	332	39	tested	test	VBN
cord-104268-q1jx0n0l	332	40	to	to	TO
cord-104268-q1jx0n0l	332	41	assess	assess	VB
cord-104268-q1jx0n0l	332	42	what	what	WP
cord-104268-q1jx0n0l	332	43	region	region	NN
cord-104268-q1jx0n0l	332	44	of	of	IN
cord-104268-q1jx0n0l	332	45	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	332	46	was	be	VBD
cord-104268-q1jx0n0l	332	47	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	332	48	to	to	TO
cord-104268-q1jx0n0l	332	49	target	target	VB
cord-104268-q1jx0n0l	332	50	a	a	DT
cord-104268-q1jx0n0l	332	51	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	332	52	reporter	reporter	NN
cord-104268-q1jx0n0l	332	53	protein	protein	NN
cord-104268-q1jx0n0l	332	54	to	to	IN
cord-104268-q1jx0n0l	332	55	the	the	DT
cord-104268-q1jx0n0l	332	56	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	332	57	complex	complex	NN
cord-104268-q1jx0n0l	332	58	.	.	.
cord-104268-q1jx0n0l	333	1	As	as	IN
cord-104268-q1jx0n0l	333	2	opposed	oppose	VBN
cord-104268-q1jx0n0l	333	3	to	to	IN
cord-104268-q1jx0n0l	333	4	the	the	DT
cord-104268-q1jx0n0l	333	5	results	result	NNS
cord-104268-q1jx0n0l	333	6	obtained	obtain	VBN
cord-104268-q1jx0n0l	333	7	with	with	IN
cord-104268-q1jx0n0l	333	8	fusion	fusion	NN
cord-104268-q1jx0n0l	333	9	protein	protein	NN
cord-104268-q1jx0n0l	333	10	KD	KD	NNP
cord-104268-q1jx0n0l	333	11	-	-	HYPH
cord-104268-q1jx0n0l	333	12	K	K	NNP
cord-104268-q1jx0n0l	333	13	,	,	,
cord-104268-q1jx0n0l	333	14	all	all	DT
cord-104268-q1jx0n0l	333	15	three	three	CD
cord-104268-q1jx0n0l	333	16	noncatalytic	noncatalytic	JJ
cord-104268-q1jx0n0l	333	17	domains	domain	NNS
cord-104268-q1jx0n0l	333	18	of	of	IN
cord-104268-q1jx0n0l	333	19	the	the	DT
cord-104268-q1jx0n0l	333	20	Kre2	Kre2	NNP
cord-104268-q1jx0n0l	333	21	protein	protein	NN
cord-104268-q1jx0n0l	333	22	were	be	VBD
cord-104268-q1jx0n0l	333	23	found	find	VBN
cord-104268-q1jx0n0l	333	24	to	to	TO
cord-104268-q1jx0n0l	333	25	be	be	VB
cord-104268-q1jx0n0l	333	26	required	require	VBN
cord-104268-q1jx0n0l	333	27	for	for	IN
cord-104268-q1jx0n0l	333	28	full	full	JJ
cord-104268-q1jx0n0l	333	29	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	333	30	retention	retention	NN
cord-104268-q1jx0n0l	333	31	of	of	IN
cord-104268-q1jx0n0l	333	32	the	the	DT
cord-104268-q1jx0n0l	333	33	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	333	34	luminal	luminal	JJ
cord-104268-q1jx0n0l	333	35	region	region	NN
cord-104268-q1jx0n0l	333	36	(	(	-LRB-
cord-104268-q1jx0n0l	333	37	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	333	38	)	)	-RRB-
cord-104268-q1jx0n0l	333	39	.	.	.
cord-104268-q1jx0n0l	334	1	These	these	DT
cord-104268-q1jx0n0l	334	2	results	result	NNS
cord-104268-q1jx0n0l	334	3	are	be	VBP
cord-104268-q1jx0n0l	334	4	in	in	IN
cord-104268-q1jx0n0l	334	5	agreement	agreement	NN
cord-104268-q1jx0n0l	334	6	with	with	IN
cord-104268-q1jx0n0l	334	7	those	those	DT
cord-104268-q1jx0n0l	334	8	of	of	IN
cord-104268-q1jx0n0l	334	9	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	334	10	and	and	CC
cord-104268-q1jx0n0l	334	11	Munro	Munro	NNP
cord-104268-q1jx0n0l	334	12	(	(	-LRB-
cord-104268-q1jx0n0l	334	13	1994	1994	CD
cord-104268-q1jx0n0l	334	14	)	)	-RRB-
cord-104268-q1jx0n0l	334	15	who	who	WP
cord-104268-q1jx0n0l	334	16	found	find	VBD
cord-104268-q1jx0n0l	334	17	that	that	IN
cord-104268-q1jx0n0l	334	18	a	a	DT
cord-104268-q1jx0n0l	334	19	fusion	fusion	NN
cord-104268-q1jx0n0l	334	20	protein	protein	NN
cord-104268-q1jx0n0l	334	21	containing	contain	VBG
cord-104268-q1jx0n0l	334	22	the	the	DT
cord-104268-q1jx0n0l	334	23	NHz	NHz	NNP
cord-104268-q1jx0n0l	334	24	terminus	terminus	NN
cord-104268-q1jx0n0l	334	25	,	,	,
cord-104268-q1jx0n0l	334	26	the	the	DT
cord-104268-q1jx0n0l	334	27	membrane	membrane	NN
cord-104268-q1jx0n0l	334	28	-	-	HYPH
cord-104268-q1jx0n0l	334	29	spanning	span	VBG
cord-104268-q1jx0n0l	334	30	domain	domain	NN
cord-104268-q1jx0n0l	334	31	,	,	,
cord-104268-q1jx0n0l	334	32	and	and	CC
cord-104268-q1jx0n0l	334	33	a	a	DT
cord-104268-q1jx0n0l	334	34	partial	partial	JJ
cord-104268-q1jx0n0l	334	35	stem	stem	NN
cord-104268-q1jx0n0l	334	36	region	region	NN
cord-104268-q1jx0n0l	334	37	of	of	IN
cord-104268-q1jx0n0l	334	38	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	334	39	linked	link	VBN
cord-104268-q1jx0n0l	334	40	to	to	IN
cord-104268-q1jx0n0l	334	41	a	a	DT
cord-104268-q1jx0n0l	334	42	reporter	reporter	NN
cord-104268-q1jx0n0l	334	43	protein	protein	NN
cord-104268-q1jx0n0l	334	44	was	be	VBD
cord-104268-q1jx0n0l	334	45	retained	retain	VBN
cord-104268-q1jx0n0l	334	46	in	in	IN
cord-104268-q1jx0n0l	334	47	the	the	DT
cord-104268-q1jx0n0l	334	48	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	334	49	complex	complex	NN
cord-104268-q1jx0n0l	334	50	.	.	.
cord-104268-q1jx0n0l	335	1	The	the	DT
cord-104268-q1jx0n0l	335	2	mostly	mostly	RB
cord-104268-q1jx0n0l	335	3	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	335	4	localization	localization	NN
cord-104268-q1jx0n0l	335	5	of	of	IN
cord-104268-q1jx0n0l	335	6	KKP	KKP	NNP
cord-104268-q1jx0n0l	335	7	demonstrated	demonstrate	VBD
cord-104268-q1jx0n0l	335	8	that	that	IN
cord-104268-q1jx0n0l	335	9	the	the	DT
cord-104268-q1jx0n0l	335	10	first	first	JJ
cord-104268-q1jx0n0l	335	11	part	part	NN
cord-104268-q1jx0n0l	335	12	of	of	IN
cord-104268-q1jx0n0l	335	13	the	the	DT
cord-104268-q1jx0n0l	335	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	335	15	stem	stem	NN
cord-104268-q1jx0n0l	335	16	region	region	NN
cord-104268-q1jx0n0l	335	17	which	which	WDT
cord-104268-q1jx0n0l	335	18	is	be	VBZ
cord-104268-q1jx0n0l	335	19	not	not	RB
cord-104268-q1jx0n0l	335	20	required	require	VBN
cord-104268-q1jx0n0l	335	21	for	for	IN
cord-104268-q1jx0n0l	335	22	retention	retention	NN
cord-104268-q1jx0n0l	335	23	in	in	IN
cord-104268-q1jx0n0l	335	24	the	the	DT
cord-104268-q1jx0n0l	335	25	context	context	NN
cord-104268-q1jx0n0l	335	26	of	of	IN
cord-104268-q1jx0n0l	335	27	a	a	DT
cord-104268-q1jx0n0l	335	28	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	335	29	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	335	30	domain	domain	NN
cord-104268-q1jx0n0l	335	31	(	(	-LRB-
cord-104268-q1jx0n0l	335	32	KD	KD	NNP
cord-104268-q1jx0n0l	335	33	-	-	HYPH
cord-104268-q1jx0n0l	335	34	K	K	NNP
cord-104268-q1jx0n0l	335	35	)	)	-RRB-
cord-104268-q1jx0n0l	335	36	,	,	,
cord-104268-q1jx0n0l	335	37	is	be	VBZ
cord-104268-q1jx0n0l	335	38	necessary	necessary	JJ
cord-104268-q1jx0n0l	335	39	,	,	,
cord-104268-q1jx0n0l	335	40	in	in	IN
cord-104268-q1jx0n0l	335	41	combination	combination	NN
cord-104268-q1jx0n0l	335	42	with	with	IN
cord-104268-q1jx0n0l	335	43	the	the	DT
cord-104268-q1jx0n0l	335	44	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	335	45	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	335	46	tail	tail	NN
cord-104268-q1jx0n0l	335	47	and	and	CC
cord-104268-q1jx0n0l	335	48	TMD	TMD	NNP
cord-104268-q1jx0n0l	335	49	for	for	IN
cord-104268-q1jx0n0l	335	50	the	the	DT
cord-104268-q1jx0n0l	335	51	targeting	targeting	NN
cord-104268-q1jx0n0l	335	52	of	of	IN
cord-104268-q1jx0n0l	335	53	the	the	DT
cord-104268-q1jx0n0l	335	54	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	335	55	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	335	56	portion	portion	NN
cord-104268-q1jx0n0l	335	57	to	to	IN
cord-104268-q1jx0n0l	335	58	the	the	DT
cord-104268-q1jx0n0l	335	59	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	335	60	complex	complex	NN
cord-104268-q1jx0n0l	335	61	(	(	-LRB-
cord-104268-q1jx0n0l	335	62	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	335	63	)	)	-RRB-
cord-104268-q1jx0n0l	335	64	.	.	.
cord-104268-q1jx0n0l	336	1	Taken	take	VBN
cord-104268-q1jx0n0l	336	2	together	together	RB
cord-104268-q1jx0n0l	336	3	,	,	,
cord-104268-q1jx0n0l	336	4	the	the	DT
cord-104268-q1jx0n0l	336	5	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	336	6	localizations	localization	NNS
cord-104268-q1jx0n0l	336	7	of	of	IN
cord-104268-q1jx0n0l	336	8	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	336	9	proteins	protein	NNS
cord-104268-q1jx0n0l	336	10	KD	KD	NNP
cord-104268-q1jx0n0l	336	11	-	-	HYPH
cord-104268-q1jx0n0l	336	12	K	K	NNP
cord-104268-q1jx0n0l	336	13	,	,	,
cord-104268-q1jx0n0l	336	14	KKP	KKP	NNP
cord-104268-q1jx0n0l	336	15	,	,	,
cord-104268-q1jx0n0l	336	16	and	and	CC
cord-104268-q1jx0n0l	336	17	KKKP	KKKP	NNS
cord-104268-q1jx0n0l	336	18	suggest	suggest	VBP
cord-104268-q1jx0n0l	336	19	that	that	IN
cord-104268-q1jx0n0l	336	20	the	the	DT
cord-104268-q1jx0n0l	336	21	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	336	22	luminal	luminal	JJ
cord-104268-q1jx0n0l	336	23	domain	domain	NN
cord-104268-q1jx0n0l	336	24	does	do	VBZ
cord-104268-q1jx0n0l	336	25	play	play	VB
cord-104268-q1jx0n0l	336	26	a	a	DT
cord-104268-q1jx0n0l	336	27	role	role	NN
cord-104268-q1jx0n0l	336	28	in	in	IN
cord-104268-q1jx0n0l	336	29	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	336	30	localization	localization	NN
cord-104268-q1jx0n0l	336	31	.	.	.
cord-104268-q1jx0n0l	337	1	Data	datum	NNS
cord-104268-q1jx0n0l	337	2	obtained	obtain	VBN
cord-104268-q1jx0n0l	337	3	with	with	IN
cord-104268-q1jx0n0l	337	4	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	337	5	proteins	protein	NNS
cord-104268-q1jx0n0l	337	6	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	337	7	,	,	,
cord-104268-q1jx0n0l	337	8	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	337	9	,	,	,
cord-104268-q1jx0n0l	337	10	KD	KD	NNP
cord-104268-q1jx0n0l	337	11	-	-	HYPH
cord-104268-q1jx0n0l	337	12	K	K	NNP
cord-104268-q1jx0n0l	337	13	,	,	,
cord-104268-q1jx0n0l	337	14	and	and	CC
cord-104268-q1jx0n0l	337	15	KKP	KKP	NNP
cord-104268-q1jx0n0l	337	16	clearly	clearly	RB
cord-104268-q1jx0n0l	337	17	show	show	VBP
cord-104268-q1jx0n0l	337	18	that	that	IN
cord-104268-q1jx0n0l	337	19	the	the	DT
cord-104268-q1jx0n0l	337	20	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	337	21	TMD	TMD	NNP
cord-104268-q1jx0n0l	337	22	is	be	VBZ
cord-104268-q1jx0n0l	337	23	dispensable	dispensable	JJ
cord-104268-q1jx0n0l	337	24	for	for	IN
cord-104268-q1jx0n0l	337	25	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	337	26	retention	retention	NN
cord-104268-q1jx0n0l	337	27	and	and	CC
cord-104268-q1jx0n0l	337	28	not	not	RB
cord-104268-q1jx0n0l	337	29	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	337	30	to	to	TO
cord-104268-q1jx0n0l	337	31	retain	retain	VB
cord-104268-q1jx0n0l	337	32	a	a	DT
cord-104268-q1jx0n0l	337	33	reporter	reporter	NN
cord-104268-q1jx0n0l	337	34	protein	protein	NN
cord-104268-q1jx0n0l	337	35	in	in	IN
cord-104268-q1jx0n0l	337	36	the	the	DT
cord-104268-q1jx0n0l	337	37	yeast	yeast	NN
cord-104268-q1jx0n0l	337	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	337	39	complex	complex	JJ
cord-104268-q1jx0n0l	337	40	.	.	.
cord-104268-q1jx0n0l	338	1	Our	-PRON-	PRP$
cord-104268-q1jx0n0l	338	2	results	result	NNS
cord-104268-q1jx0n0l	338	3	are	be	VBP
cord-104268-q1jx0n0l	338	4	,	,	,
cord-104268-q1jx0n0l	338	5	however	however	RB
cord-104268-q1jx0n0l	338	6	,	,	,
cord-104268-q1jx0n0l	338	7	at	at	IN
cord-104268-q1jx0n0l	338	8	variance	variance	NN
cord-104268-q1jx0n0l	338	9	with	with	IN
cord-104268-q1jx0n0l	338	10	conclusions	conclusion	NNS
cord-104268-q1jx0n0l	338	11	reached	reach	VBN
cord-104268-q1jx0n0l	338	12	in	in	IN
cord-104268-q1jx0n0l	338	13	a	a	DT
cord-104268-q1jx0n0l	338	14	recent	recent	JJ
cord-104268-q1jx0n0l	338	15	study	study	NN
cord-104268-q1jx0n0l	338	16	of	of	IN
cord-104268-q1jx0n0l	338	17	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	338	18	and	and	CC
cord-104268-q1jx0n0l	338	19	Munro	Munro	NNP
cord-104268-q1jx0n0l	338	20	(	(	-LRB-
cord-104268-q1jx0n0l	338	21	1994	1994	CD
cord-104268-q1jx0n0l	338	22	)	)	-RRB-
cord-104268-q1jx0n0l	338	23	and	and	CC
cord-104268-q1jx0n0l	338	24	we	-PRON-	PRP
cord-104268-q1jx0n0l	338	25	address	address	VBP
cord-104268-q1jx0n0l	338	26	this	this	DT
cord-104268-q1jx0n0l	338	27	below	below	RB
cord-104268-q1jx0n0l	338	28	.	.	.
cord-104268-q1jx0n0l	339	1	In	in	IN
cord-104268-q1jx0n0l	339	2	a	a	DT
cord-104268-q1jx0n0l	339	3	first	first	JJ
cord-104268-q1jx0n0l	339	4	set	set	NN
cord-104268-q1jx0n0l	339	5	of	of	IN
cord-104268-q1jx0n0l	339	6	experiments	experiment	NNS
cord-104268-q1jx0n0l	339	7	,	,	,
cord-104268-q1jx0n0l	339	8	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	339	9	and	and	CC
cord-104268-q1jx0n0l	339	10	Munro	Munro	NNP
cord-104268-q1jx0n0l	339	11	(	(	-LRB-
cord-104268-q1jx0n0l	339	12	1994	1994	CD
cord-104268-q1jx0n0l	339	13	)	)	-RRB-
cord-104268-q1jx0n0l	339	14	tested	test	VBD
cord-104268-q1jx0n0l	339	15	the	the	DT
cord-104268-q1jx0n0l	339	16	role	role	NN
cord-104268-q1jx0n0l	339	17	of	of	IN
cord-104268-q1jx0n0l	339	18	the	the	DT
cord-104268-q1jx0n0l	339	19	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	339	20	TMD	TMD	NNP
cord-104268-q1jx0n0l	339	21	with	with	IN
cord-104268-q1jx0n0l	339	22	a	a	DT
cord-104268-q1jx0n0l	339	23	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	339	24	protein	protein	NN
cord-104268-q1jx0n0l	339	25	consisting	consist	VBG
cord-104268-q1jx0n0l	339	26	of	of	IN
cord-104268-q1jx0n0l	339	27	a	a	DT
cord-104268-q1jx0n0l	339	28	full	full	JJ
cord-104268-q1jx0n0l	339	29	-	-	HYPH
cord-104268-q1jx0n0l	339	30	length	length	NN
cord-104268-q1jx0n0l	339	31	fusion	fusion	NN
cord-104268-q1jx0n0l	339	32	of	of	IN
cord-104268-q1jx0n0l	339	33	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	339	34	/	/	SYM
cord-104268-q1jx0n0l	339	35	Mntlp	Mntlp	NNP
cord-104268-q1jx0n0l	339	36	linked	link	VBD
cord-104268-q1jx0n0l	339	37	to	to	IN
cord-104268-q1jx0n0l	339	38	the	the	DT
cord-104268-q1jx0n0l	339	39	SUC2-encoded	SUC2-encoded	NNP
cord-104268-q1jx0n0l	339	40	invertase	invertase	NN
cord-104268-q1jx0n0l	339	41	by	by	IN
cord-104268-q1jx0n0l	339	42	a	a	DT
cord-104268-q1jx0n0l	339	43	small	small	JJ
cord-104268-q1jx0n0l	339	44	region	region	NN
cord-104268-q1jx0n0l	339	45	containing	contain	VBG
cord-104268-q1jx0n0l	339	46	a	a	DT
cord-104268-q1jx0n0l	339	47	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	339	48	-	-	HYPH
cord-104268-q1jx0n0l	339	49	dependent	dependent	JJ
cord-104268-q1jx0n0l	339	50	cleavage	cleavage	NN
cord-104268-q1jx0n0l	339	51	site	site	NN
cord-104268-q1jx0n0l	339	52	(	(	-LRB-
cord-104268-q1jx0n0l	339	53	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	339	54	protein	protein	NN
cord-104268-q1jx0n0l	339	55	MMMI	MMMI	NNP
cord-104268-q1jx0n0l	339	56	)	)	-RRB-
cord-104268-q1jx0n0l	339	57	.	.	.
cord-104268-q1jx0n0l	340	1	The	the	DT
cord-104268-q1jx0n0l	340	2	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	340	3	of	of	IN
cord-104268-q1jx0n0l	340	4	this	this	DT
cord-104268-q1jx0n0l	340	5	protein	protein	NN
cord-104268-q1jx0n0l	340	6	was	be	VBD
cord-104268-q1jx0n0l	340	7	expected	expect	VBN
cord-104268-q1jx0n0l	340	8	to	to	TO
cord-104268-q1jx0n0l	340	9	result	result	VB
cord-104268-q1jx0n0l	340	10	in	in	IN
cord-104268-q1jx0n0l	340	11	production	production	NN
cord-104268-q1jx0n0l	340	12	of	of	IN
cord-104268-q1jx0n0l	340	13	soluble	soluble	JJ
cord-104268-q1jx0n0l	340	14	invertase	invertase	NN
cord-104268-q1jx0n0l	340	15	when	when	WRB
cord-104268-q1jx0n0l	340	16	passing	pass	VBG
cord-104268-q1jx0n0l	340	17	through	through	IN
cord-104268-q1jx0n0l	340	18	the	the	DT
cord-104268-q1jx0n0l	340	19	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	340	20	-	-	HYPH
cord-104268-q1jx0n0l	340	21	containing	contain	VBG
cord-104268-q1jx0n0l	340	22	late	late	JJ
cord-104268-q1jx0n0l	340	23	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	340	24	compartment	compartment	NN
cord-104268-q1jx0n0l	340	25	.	.	.
cord-104268-q1jx0n0l	341	1	This	this	DT
cord-104268-q1jx0n0l	341	2	fusion	fusion	NN
cord-104268-q1jx0n0l	341	3	protein	protein	NN
cord-104268-q1jx0n0l	341	4	was	be	VBD
cord-104268-q1jx0n0l	341	5	inferred	infer	VBN
cord-104268-q1jx0n0l	341	6	to	to	TO
cord-104268-q1jx0n0l	341	7	be	be	VB
cord-104268-q1jx0n0l	341	8	in	in	IN
cord-104268-q1jx0n0l	341	9	the	the	DT
cord-104268-q1jx0n0l	341	10	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	341	11	complex	complex	NN
cord-104268-q1jx0n0l	341	12	as	as	IN
cord-104268-q1jx0n0l	341	13	no	no	DT
cord-104268-q1jx0n0l	341	14	invertase	invertase	NN
cord-104268-q1jx0n0l	341	15	was	be	VBD
cord-104268-q1jx0n0l	341	16	secreted	secrete	VBN
cord-104268-q1jx0n0l	341	17	from	from	IN
cord-104268-q1jx0n0l	341	18	the	the	DT
cord-104268-q1jx0n0l	341	19	cell	cell	NN
cord-104268-q1jx0n0l	341	20	.	.	.
cord-104268-q1jx0n0l	342	1	When	when	WRB
cord-104268-q1jx0n0l	342	2	the	the	DT
cord-104268-q1jx0n0l	342	3	TMD	TMD	NNP
cord-104268-q1jx0n0l	342	4	of	of	IN
cord-104268-q1jx0n0l	342	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	342	6	was	be	VBD
cord-104268-q1jx0n0l	342	7	replaced	replace	VBN
cord-104268-q1jx0n0l	342	8	with	with	IN
cord-104268-q1jx0n0l	342	9	that	that	DT
cord-104268-q1jx0n0l	342	10	of	of	IN
cord-104268-q1jx0n0l	342	11	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	342	12	(	(	-LRB-
cord-104268-q1jx0n0l	342	13	MPMI	MPMI	NNP
cord-104268-q1jx0n0l	342	14	)	)	-RRB-
cord-104268-q1jx0n0l	342	15	in	in	IN
cord-104268-q1jx0n0l	342	16	this	this	DT
cord-104268-q1jx0n0l	342	17	protein	protein	NN
cord-104268-q1jx0n0l	342	18	,	,	,
cord-104268-q1jx0n0l	342	19	it	-PRON-	PRP
cord-104268-q1jx0n0l	342	20	appeared	appear	VBD
cord-104268-q1jx0n0l	342	21	to	to	TO
cord-104268-q1jx0n0l	342	22	be	be	VB
cord-104268-q1jx0n0l	342	23	mislocalized	mislocalize	VBN
cord-104268-q1jx0n0l	342	24	to	to	IN
cord-104268-q1jx0n0l	342	25	some	some	DT
cord-104268-q1jx0n0l	342	26	extent	extent	NN
cord-104268-q1jx0n0l	342	27	,	,	,
cord-104268-q1jx0n0l	342	28	as	as	IN
cord-104268-q1jx0n0l	342	29	some	some	DT
cord-104268-q1jx0n0l	342	30	invertase	invertase	NN
cord-104268-q1jx0n0l	342	31	was	be	VBD
cord-104268-q1jx0n0l	342	32	secreted	secrete	VBN
cord-104268-q1jx0n0l	342	33	from	from	IN
cord-104268-q1jx0n0l	342	34	the	the	DT
cord-104268-q1jx0n0l	342	35	cell	cell	NN
cord-104268-q1jx0n0l	342	36	.	.	.
cord-104268-q1jx0n0l	343	1	This	this	DT
cord-104268-q1jx0n0l	343	2	result	result	NN
cord-104268-q1jx0n0l	343	3	was	be	VBD
cord-104268-q1jx0n0l	343	4	interpreted	interpret	VBN
cord-104268-q1jx0n0l	343	5	to	to	TO
cord-104268-q1jx0n0l	343	6	indicate	indicate	VB
cord-104268-q1jx0n0l	343	7	a	a	DT
cord-104268-q1jx0n0l	343	8	role	role	NN
cord-104268-q1jx0n0l	343	9	for	for	IN
cord-104268-q1jx0n0l	343	10	the	the	DT
cord-104268-q1jx0n0l	343	11	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	343	12	TMD	TMD	NNP
cord-104268-q1jx0n0l	343	13	in	in	IN
cord-104268-q1jx0n0l	343	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	343	15	retention	retention	NN
cord-104268-q1jx0n0l	343	16	.	.	.
cord-104268-q1jx0n0l	344	1	However	however	RB
cord-104268-q1jx0n0l	344	2	,	,	,
cord-104268-q1jx0n0l	344	3	the	the	DT
cord-104268-q1jx0n0l	344	4	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	344	5	localization	localization	NN
cord-104268-q1jx0n0l	344	6	of	of	IN
cord-104268-q1jx0n0l	344	7	this	this	DT
cord-104268-q1jx0n0l	344	8	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	344	9	protein	protein	NN
cord-104268-q1jx0n0l	344	10	was	be	VBD
cord-104268-q1jx0n0l	344	11	not	not	RB
cord-104268-q1jx0n0l	344	12	visualized	visualize	VBN
cord-104268-q1jx0n0l	344	13	by	by	IN
cord-104268-q1jx0n0l	344	14	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	344	15	.	.	.
cord-104268-q1jx0n0l	345	1	We	-PRON-	PRP
cord-104268-q1jx0n0l	345	2	replaced	replace	VBD
cord-104268-q1jx0n0l	345	3	the	the	DT
cord-104268-q1jx0n0l	345	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	345	5	TMD	TMD	NNP
cord-104268-q1jx0n0l	345	6	in	in	IN
cord-104268-q1jx0n0l	345	7	the	the	DT
cord-104268-q1jx0n0l	345	8	native	native	JJ
cord-104268-q1jx0n0l	345	9	protein	protein	NN
cord-104268-q1jx0n0l	345	10	with	with	IN
cord-104268-q1jx0n0l	345	11	two	two	CD
cord-104268-q1jx0n0l	345	12	different	different	JJ
cord-104268-q1jx0n0l	345	13	nonrelated	nonrelated	JJ
cord-104268-q1jx0n0l	345	14	TMD	TMD	NNP
cord-104268-q1jx0n0l	345	15	's	's	POS
cord-104268-q1jx0n0l	345	16	,	,	,
cord-104268-q1jx0n0l	345	17	and	and	CC
cord-104268-q1jx0n0l	345	18	showed	show	VBD
cord-104268-q1jx0n0l	345	19	that	that	IN
cord-104268-q1jx0n0l	345	20	both	both	DT
cord-104268-q1jx0n0l	345	21	proteins	protein	NNS
cord-104268-q1jx0n0l	345	22	(	(	-LRB-
cord-104268-q1jx0n0l	345	23	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	345	24	and	and	CC
cord-104268-q1jx0n0l	345	25	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	345	26	)	)	-RRB-
cord-104268-q1jx0n0l	345	27	were	be	VBD
cord-104268-q1jx0n0l	345	28	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	345	29	localized	localize	VBN
cord-104268-q1jx0n0l	345	30	by	by	IN
cord-104268-q1jx0n0l	345	31	using	use	VBG
cord-104268-q1jx0n0l	345	32	indirect	indirect	JJ
cord-104268-q1jx0n0l	345	33	immunofluorescence	immunofluorescence	NN
cord-104268-q1jx0n0l	345	34	microscopy	microscopy	NN
cord-104268-q1jx0n0l	345	35	.	.	.
cord-104268-q1jx0n0l	346	1	In	in	IN
cord-104268-q1jx0n0l	346	2	addition	addition	NN
cord-104268-q1jx0n0l	346	3	,	,	,
cord-104268-q1jx0n0l	346	4	we	-PRON-	PRP
cord-104268-q1jx0n0l	346	5	tested	test	VBD
cord-104268-q1jx0n0l	346	6	that	that	IN
cord-104268-q1jx0n0l	346	7	the	the	DT
cord-104268-q1jx0n0l	346	8	proteins	protein	NNS
cord-104268-q1jx0n0l	346	9	had	have	VBD
cord-104268-q1jx0n0l	346	10	the	the	DT
cord-104268-q1jx0n0l	346	11	right	right	JJ
cord-104268-q1jx0n0l	346	12	conformation	conformation	NN
cord-104268-q1jx0n0l	346	13	by	by	IN
cord-104268-q1jx0n0l	346	14	measuring	measure	VBG
cord-104268-q1jx0n0l	346	15	their	-PRON-	PRP$
cord-104268-q1jx0n0l	346	16	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	346	17	in	in	IN
cord-104268-q1jx0n0l	346	18	vivo	vivo	NN
cord-104268-q1jx0n0l	346	19	,	,	,
cord-104268-q1jx0n0l	346	20	and	and	CC
cord-104268-q1jx0n0l	346	21	their	-PRON-	PRP$
cord-104268-q1jx0n0l	346	22	mannosyltransferase	mannosyltransferase	JJ
cord-104268-q1jx0n0l	346	23	activity	activity	NN
cord-104268-q1jx0n0l	346	24	in	in	IN
cord-104268-q1jx0n0l	346	25	vitro	vitro	FW
cord-104268-q1jx0n0l	346	26	and	and	CC
cord-104268-q1jx0n0l	346	27	in	in	IN
cord-104268-q1jx0n0l	346	28	vivo	vivo	NNP
cord-104268-q1jx0n0l	346	29	.	.	.
cord-104268-q1jx0n0l	347	1	These	these	DT
cord-104268-q1jx0n0l	347	2	proteins	protein	NNS
cord-104268-q1jx0n0l	347	3	(	(	-LRB-
cord-104268-q1jx0n0l	347	4	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	347	5	and	and	CC
cord-104268-q1jx0n0l	347	6	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	347	7	)	)	-RRB-
cord-104268-q1jx0n0l	347	8	behaved	behave	VBD
cord-104268-q1jx0n0l	347	9	phenotypically	phenotypically	RB
cord-104268-q1jx0n0l	347	10	as	as	IN
cord-104268-q1jx0n0l	347	11	wild	wild	JJ
cord-104268-q1jx0n0l	347	12	-	-	HYPH
cord-104268-q1jx0n0l	347	13	type	type	NN
cord-104268-q1jx0n0l	347	14	Kre2p	kre2p	XX
cord-104268-q1jx0n0l	347	15	in	in	IN
cord-104268-q1jx0n0l	347	16	our	-PRON-	PRP$
cord-104268-q1jx0n0l	347	17	tests	test	NNS
cord-104268-q1jx0n0l	347	18	with	with	IN
cord-104268-q1jx0n0l	347	19	no	no	DT
cord-104268-q1jx0n0l	347	20	indication	indication	NN
cord-104268-q1jx0n0l	347	21	that	that	IN
cord-104268-q1jx0n0l	347	22	the	the	DT
cord-104268-q1jx0n0l	347	23	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	347	24	TMD	TMD	NNP
cord-104268-q1jx0n0l	347	25	in	in	IN
cord-104268-q1jx0n0l	347	26	the	the	DT
cord-104268-q1jx0n0l	347	27	context	context	NN
cord-104268-q1jx0n0l	347	28	of	of	IN
cord-104268-q1jx0n0l	347	29	a	a	DT
cord-104268-q1jx0n0l	347	30	native	native	JJ
cord-104268-q1jx0n0l	347	31	protein	protein	NN
cord-104268-q1jx0n0l	347	32	is	be	VBZ
cord-104268-q1jx0n0l	347	33	necessary	necessary	JJ
cord-104268-q1jx0n0l	347	34	for	for	IN
cord-104268-q1jx0n0l	347	35	proper	proper	JJ
cord-104268-q1jx0n0l	347	36	retention	retention	NN
cord-104268-q1jx0n0l	347	37	.	.	.
cord-104268-q1jx0n0l	348	1	A	a	DT
cord-104268-q1jx0n0l	348	2	possible	possible	JJ
cord-104268-q1jx0n0l	348	3	explanation	explanation	NN
cord-104268-q1jx0n0l	348	4	of	of	IN
cord-104268-q1jx0n0l	348	5	the	the	DT
cord-104268-q1jx0n0l	348	6	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	348	7	and	and	CC
cord-104268-q1jx0n0l	348	8	Munro	Munro	NNP
cord-104268-q1jx0n0l	348	9	(	(	-LRB-
cord-104268-q1jx0n0l	348	10	1994	1994	CD
cord-104268-q1jx0n0l	348	11	)	)	-RRB-
cord-104268-q1jx0n0l	348	12	results	result	NNS
cord-104268-q1jx0n0l	348	13	is	be	VBZ
cord-104268-q1jx0n0l	348	14	that	that	IN
cord-104268-q1jx0n0l	348	15	the	the	DT
cord-104268-q1jx0n0l	348	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	348	17	TMD	TMD	NNP
cord-104268-q1jx0n0l	348	18	is	be	VBZ
cord-104268-q1jx0n0l	348	19	somehow	somehow	RB
cord-104268-q1jx0n0l	348	20	necessary	necessary	JJ
cord-104268-q1jx0n0l	348	21	for	for	IN
cord-104268-q1jx0n0l	348	22	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	348	23	retention	retention	NN
cord-104268-q1jx0n0l	348	24	in	in	IN
cord-104268-q1jx0n0l	348	25	the	the	DT
cord-104268-q1jx0n0l	348	26	context	context	NN
cord-104268-q1jx0n0l	348	27	of	of	IN
cord-104268-q1jx0n0l	348	28	those	those	DT
cord-104268-q1jx0n0l	348	29	large	large	JJ
cord-104268-q1jx0n0l	348	30	heterologous	heterologous	JJ
cord-104268-q1jx0n0l	348	31	proteins	protein	NNS
cord-104268-q1jx0n0l	348	32	perhaps	perhaps	RB
cord-104268-q1jx0n0l	348	33	because	because	IN
cord-104268-q1jx0n0l	348	34	some	some	DT
cord-104268-q1jx0n0l	348	35	retention	retention	NN
cord-104268-q1jx0n0l	348	36	property	property	NN
cord-104268-q1jx0n0l	348	37	of	of	IN
cord-104268-q1jx0n0l	348	38	the	the	DT
cord-104268-q1jx0n0l	348	39	luminal	luminal	JJ
cord-104268-q1jx0n0l	348	40	domain	domain	NN
cord-104268-q1jx0n0l	348	41	of	of	IN
cord-104268-q1jx0n0l	348	42	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	348	43	has	have	VBZ
cord-104268-q1jx0n0l	348	44	been	be	VBN
cord-104268-q1jx0n0l	348	45	disrupted	disrupt	VBN
cord-104268-q1jx0n0l	348	46	in	in	IN
cord-104268-q1jx0n0l	348	47	the	the	DT
cord-104268-q1jx0n0l	348	48	invertase	invertase	NNP
cord-104268-q1jx0n0l	348	49	fusion	fusion	NN
cord-104268-q1jx0n0l	348	50	.	.	.
cord-104268-q1jx0n0l	349	1	Unfortunately	unfortunately	RB
cord-104268-q1jx0n0l	349	2	,	,	,
cord-104268-q1jx0n0l	349	3	the	the	DT
cord-104268-q1jx0n0l	349	4	ER	ER	NNP
cord-104268-q1jx0n0l	349	5	retention	retention	NN
cord-104268-q1jx0n0l	349	6	of	of	IN
cord-104268-q1jx0n0l	349	7	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	349	8	protein	protein	NN
cord-104268-q1jx0n0l	349	9	KPKP	KPKP	NNP
cord-104268-q1jx0n0l	349	10	did	do	VBD
cord-104268-q1jx0n0l	349	11	not	not	RB
cord-104268-q1jx0n0l	349	12	permit	permit	VB
cord-104268-q1jx0n0l	349	13	resolution	resolution	NN
cord-104268-q1jx0n0l	349	14	of	of	IN
cord-104268-q1jx0n0l	349	15	this	this	DT
cord-104268-q1jx0n0l	349	16	question	question	NN
cord-104268-q1jx0n0l	349	17	.	.	.
cord-104268-q1jx0n0l	350	1	Second	second	RB
cord-104268-q1jx0n0l	350	2	,	,	,
cord-104268-q1jx0n0l	350	3	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	350	4	and	and	CC
cord-104268-q1jx0n0l	350	5	Munro	Munro	NNP
cord-104268-q1jx0n0l	350	6	(	(	-LRB-
cord-104268-q1jx0n0l	350	7	1994	1994	CD
cord-104268-q1jx0n0l	350	8	)	)	-RRB-
cord-104268-q1jx0n0l	350	9	reported	report	VBD
cord-104268-q1jx0n0l	350	10	that	that	IN
cord-104268-q1jx0n0l	350	11	a	a	DT
cord-104268-q1jx0n0l	350	12	Pho8	Pho8	NNP
cord-104268-q1jx0n0l	350	13	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	350	14	protein	protein	NN
cord-104268-q1jx0n0l	350	15	containing	contain	VBG
cord-104268-q1jx0n0l	350	16	a	a	DT
cord-104268-q1jx0n0l	350	17	Kre2p	Kre2p	VBN
cord-104268-q1jx0n0l	350	18	TMD	TMD	NNP
cord-104268-q1jx0n0l	350	19	(	(	-LRB-
cord-104268-q1jx0n0l	350	20	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	350	21	protein	protein	NN
cord-104268-q1jx0n0l	350	22	PKP	PKP	NNP
cord-104268-q1jx0n0l	350	23	)	)	-RRB-
cord-104268-q1jx0n0l	350	24	slowed	slow	VBD
cord-104268-q1jx0n0l	350	25	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	350	26	processing	processing	NN
cord-104268-q1jx0n0l	350	27	of	of	IN
cord-104268-q1jx0n0l	350	28	the	the	DT
cord-104268-q1jx0n0l	350	29	luminal	luminal	JJ
cord-104268-q1jx0n0l	350	30	portion	portion	NN
cord-104268-q1jx0n0l	350	31	of	of	IN
cord-104268-q1jx0n0l	350	32	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	350	33	.	.	.
cord-104268-q1jx0n0l	351	1	They	-PRON-	PRP
cord-104268-q1jx0n0l	351	2	inferred	infer	VBD
cord-104268-q1jx0n0l	351	3	from	from	IN
cord-104268-q1jx0n0l	351	4	their	-PRON-	PRP$
cord-104268-q1jx0n0l	351	5	results	result	NNS
cord-104268-q1jx0n0l	351	6	that	that	IN
cord-104268-q1jx0n0l	351	7	PKP	PKP	NNP
cord-104268-q1jx0n0l	351	8	was	be	VBD
cord-104268-q1jx0n0l	351	9	at	at	IN
cord-104268-q1jx0n0l	351	10	least	least	JJS
cord-104268-q1jx0n0l	351	11	partially	partially	RB
cord-104268-q1jx0n0l	351	12	localized	localize	VBN
cord-104268-q1jx0n0l	351	13	in	in	IN
cord-104268-q1jx0n0l	351	14	the	the	DT
cord-104268-q1jx0n0l	351	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	351	16	complex	complex	NN
cord-104268-q1jx0n0l	351	17	and	and	CC
cord-104268-q1jx0n0l	351	18	was	be	VBD
cord-104268-q1jx0n0l	351	19	retained	retain	VBN
cord-104268-q1jx0n0l	351	20	by	by	IN
cord-104268-q1jx0n0l	351	21	the	the	DT
cord-104268-q1jx0n0l	351	22	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	351	23	TMD	TMD	NNP
cord-104268-q1jx0n0l	351	24	.	.	.
cord-104268-q1jx0n0l	352	1	Again	again	RB
cord-104268-q1jx0n0l	352	2	,	,	,
cord-104268-q1jx0n0l	352	3	the	the	DT
cord-104268-q1jx0n0l	352	4	PKP	PKP	NNP
cord-104268-q1jx0n0l	352	5	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	352	6	protein	protein	NN
cord-104268-q1jx0n0l	352	7	was	be	VBD
cord-104268-q1jx0n0l	352	8	not	not	RB
cord-104268-q1jx0n0l	352	9	localized	localize	VBN
cord-104268-q1jx0n0l	352	10	intracellularly	intracellularly	RB
cord-104268-q1jx0n0l	352	11	.	.	.
cord-104268-q1jx0n0l	353	1	This	this	DT
cord-104268-q1jx0n0l	353	2	is	be	VBZ
cord-104268-q1jx0n0l	353	3	clearly	clearly	RB
cord-104268-q1jx0n0l	353	4	different	different	JJ
cord-104268-q1jx0n0l	353	5	from	from	IN
cord-104268-q1jx0n0l	353	6	our	-PRON-	PRP$
cord-104268-q1jx0n0l	353	7	results	result	NNS
cord-104268-q1jx0n0l	353	8	with	with	IN
cord-104268-q1jx0n0l	353	9	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	353	10	chimeras	chimera	NNS
cord-104268-q1jx0n0l	353	11	,	,	,
cord-104268-q1jx0n0l	353	12	which	which	WDT
cord-104268-q1jx0n0l	353	13	show	show	VBP
cord-104268-q1jx0n0l	353	14	that	that	IN
cord-104268-q1jx0n0l	353	15	the	the	DT
cord-104268-q1jx0n0l	353	16	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	353	17	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	353	18	NHz	nhz	NN
cord-104268-q1jx0n0l	353	19	-	-	HYPH
cord-104268-q1jx0n0l	353	20	terminal	terminal	NN
cord-104268-q1jx0n0l	353	21	and	and	CC
cord-104268-q1jx0n0l	353	22	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	353	23	domains	domain	NNS
cord-104268-q1jx0n0l	353	24	(	(	-LRB-
cord-104268-q1jx0n0l	353	25	KKP	KKP	NNP
cord-104268-q1jx0n0l	353	26	)	)	-RRB-
cord-104268-q1jx0n0l	353	27	are	be	VBP
cord-104268-q1jx0n0l	353	28	not	not	RB
cord-104268-q1jx0n0l	353	29	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	353	30	for	for	IN
cord-104268-q1jx0n0l	353	31	proper	proper	JJ
cord-104268-q1jx0n0l	353	32	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	353	33	retention	retention	NN
cord-104268-q1jx0n0l	353	34	and	and	CC
cord-104268-q1jx0n0l	353	35	the	the	DT
cord-104268-q1jx0n0l	353	36	presence	presence	NN
cord-104268-q1jx0n0l	353	37	of	of	IN
cord-104268-q1jx0n0l	353	38	the	the	DT
cord-104268-q1jx0n0l	353	39	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	353	40	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	353	41	tail	tail	NN
cord-104268-q1jx0n0l	353	42	,	,	,
cord-104268-q1jx0n0l	353	43	TMD	TMD	NNP
cord-104268-q1jx0n0l	353	44	,	,	,
cord-104268-q1jx0n0l	353	45	and	and	CC
cord-104268-q1jx0n0l	353	46	stem	stem	NN
cord-104268-q1jx0n0l	353	47	region	region	NN
cord-104268-q1jx0n0l	353	48	is	be	VBZ
cord-104268-q1jx0n0l	353	49	required	require	VBN
cord-104268-q1jx0n0l	353	50	for	for	IN
cord-104268-q1jx0n0l	353	51	full	full	JJ
cord-104268-q1jx0n0l	353	52	retention	retention	NN
cord-104268-q1jx0n0l	353	53	of	of	IN
cord-104268-q1jx0n0l	353	54	a	a	DT
cord-104268-q1jx0n0l	353	55	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	353	56	reporter	reporter	NN
cord-104268-q1jx0n0l	353	57	protein	protein	NN
cord-104268-q1jx0n0l	353	58	(	(	-LRB-
cord-104268-q1jx0n0l	353	59	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	353	60	)	)	-RRB-
cord-104268-q1jx0n0l	353	61	in	in	IN
cord-104268-q1jx0n0l	353	62	the	the	DT
cord-104268-q1jx0n0l	353	63	yeast	yeast	NN
cord-104268-q1jx0n0l	353	64	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	353	65	.	.	.
cord-104268-q1jx0n0l	354	1	To	to	TO
cord-104268-q1jx0n0l	354	2	resolve	resolve	VB
cord-104268-q1jx0n0l	354	3	this	this	DT
cord-104268-q1jx0n0l	354	4	apparent	apparent	JJ
cord-104268-q1jx0n0l	354	5	inconsistency	inconsistency	NN
cord-104268-q1jx0n0l	354	6	,	,	,
cord-104268-q1jx0n0l	354	7	we	-PRON-	PRP
cord-104268-q1jx0n0l	354	8	obtained	obtain	VBD
cord-104268-q1jx0n0l	354	9	the	the	DT
cord-104268-q1jx0n0l	354	10	PKP	PKP	NNP
cord-104268-q1jx0n0l	354	11	construct	construct	NN
cord-104268-q1jx0n0l	354	12	and	and	CC
cord-104268-q1jx0n0l	354	13	,	,	,
cord-104268-q1jx0n0l	354	14	on	on	IN
cord-104268-q1jx0n0l	354	15	examination	examination	NN
cord-104268-q1jx0n0l	354	16	of	of	IN
cord-104268-q1jx0n0l	354	17	its	-PRON-	PRP$
cord-104268-q1jx0n0l	354	18	cellular	cellular	JJ
cord-104268-q1jx0n0l	354	19	location	location	NN
cord-104268-q1jx0n0l	354	20	,	,	,
cord-104268-q1jx0n0l	354	21	found	find	VBD
cord-104268-q1jx0n0l	354	22	that	that	IN
cord-104268-q1jx0n0l	354	23	it	-PRON-	PRP
cord-104268-q1jx0n0l	354	24	was	be	VBD
cord-104268-q1jx0n0l	354	25	exclusively	exclusively	RB
cord-104268-q1jx0n0l	354	26	localized	localize	VBN
cord-104268-q1jx0n0l	354	27	to	to	IN
cord-104268-q1jx0n0l	354	28	the	the	DT
cord-104268-q1jx0n0l	354	29	vacuole	vacuole	NN
cord-104268-q1jx0n0l	354	30	displaying	display	VBG
cord-104268-q1jx0n0l	354	31	no	no	DT
cord-104268-q1jx0n0l	354	32	punctiform	punctiform	NN
cord-104268-q1jx0n0l	354	33	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	354	34	(	(	-LRB-
cord-104268-q1jx0n0l	354	35	data	datum	NNS
cord-104268-q1jx0n0l	354	36	not	not	RB
cord-104268-q1jx0n0l	354	37	shown	show	VBN
cord-104268-q1jx0n0l	354	38	)	)	-RRB-
cord-104268-q1jx0n0l	354	39	.	.	.
cord-104268-q1jx0n0l	355	1	This	this	DT
cord-104268-q1jx0n0l	355	2	finding	finding	NN
cord-104268-q1jx0n0l	355	3	is	be	VBZ
cord-104268-q1jx0n0l	355	4	entirely	entirely	RB
cord-104268-q1jx0n0l	355	5	consistent	consistent	JJ
cord-104268-q1jx0n0l	355	6	with	with	IN
cord-104268-q1jx0n0l	355	7	our	-PRON-	PRP$
cord-104268-q1jx0n0l	355	8	localization	localization	NN
cord-104268-q1jx0n0l	355	9	results	result	NNS
cord-104268-q1jx0n0l	355	10	with	with	IN
cord-104268-q1jx0n0l	355	11	KKP	KKP	NNP
cord-104268-q1jx0n0l	355	12	,	,	,
cord-104268-q1jx0n0l	355	13	and	and	CC
cord-104268-q1jx0n0l	355	14	offers	offer	VBZ
cord-104268-q1jx0n0l	355	15	no	no	DT
cord-104268-q1jx0n0l	355	16	support	support	NN
cord-104268-q1jx0n0l	355	17	for	for	IN
cord-104268-q1jx0n0l	355	18	the	the	DT
cord-104268-q1jx0n0l	355	19	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	355	20	and	and	CC
cord-104268-q1jx0n0l	355	21	Munro	Munro	NNP
cord-104268-q1jx0n0l	355	22	conclusion	conclusion	NN
cord-104268-q1jx0n0l	355	23	that	that	IN
cord-104268-q1jx0n0l	355	24	the	the	DT
cord-104268-q1jx0n0l	355	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	355	26	TMD	TMD	NNP
cord-104268-q1jx0n0l	355	27	will	will	MD
cord-104268-q1jx0n0l	355	28	at	at	IN
cord-104268-q1jx0n0l	355	29	least	least	RBS
cord-104268-q1jx0n0l	355	30	partially	partially	RB
cord-104268-q1jx0n0l	355	31	retain	retain	VB
cord-104268-q1jx0n0l	355	32	a	a	DT
cord-104268-q1jx0n0l	355	33	heterologous	heterologous	JJ
cord-104268-q1jx0n0l	355	34	protein	protein	NN
cord-104268-q1jx0n0l	355	35	in	in	IN
cord-104268-q1jx0n0l	355	36	the	the	DT
cord-104268-q1jx0n0l	355	37	yeast	yeast	NN
cord-104268-q1jx0n0l	355	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	355	39	.	.	.
cord-104268-q1jx0n0l	356	1	The	the	DT
cord-104268-q1jx0n0l	356	2	membrane	membrane	NN
cord-104268-q1jx0n0l	356	3	-	-	HYPH
cord-104268-q1jx0n0l	356	4	spanning	span	VBG
cord-104268-q1jx0n0l	356	5	domain	domain	NN
cord-104268-q1jx0n0l	356	6	plays	play	VBZ
cord-104268-q1jx0n0l	356	7	a	a	DT
cord-104268-q1jx0n0l	356	8	crucial	crucial	JJ
cord-104268-q1jx0n0l	356	9	role	role	NN
cord-104268-q1jx0n0l	356	10	in	in	IN
cord-104268-q1jx0n0l	356	11	the	the	DT
cord-104268-q1jx0n0l	356	12	localization	localization	NN
cord-104268-q1jx0n0l	356	13	of	of	IN
cord-104268-q1jx0n0l	356	14	resident	resident	JJ
cord-104268-q1jx0n0l	356	15	animal	animal	NN
cord-104268-q1jx0n0l	356	16	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	356	17	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	356	18	.	.	.
cord-104268-q1jx0n0l	357	1	In	in	IN
cord-104268-q1jx0n0l	357	2	contrast	contrast	NN
cord-104268-q1jx0n0l	357	3	to	to	IN
cord-104268-q1jx0n0l	357	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	357	5	,	,	,
cord-104268-q1jx0n0l	357	6	alterations	alteration	NNS
cord-104268-q1jx0n0l	357	7	of	of	IN
cord-104268-q1jx0n0l	357	8	the	the	DT
cord-104268-q1jx0n0l	357	9	TMD	TMD	NNP
cord-104268-q1jx0n0l	357	10	of	of	IN
cord-104268-q1jx0n0l	357	11	a	a	DT
cord-104268-q1jx0n0l	357	12	certain	certain	JJ
cord-104268-q1jx0n0l	357	13	number	number	NN
cord-104268-q1jx0n0l	357	14	of	of	IN
cord-104268-q1jx0n0l	357	15	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	357	16	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	357	17	results	result	NNS
cord-104268-q1jx0n0l	357	18	in	in	IN
cord-104268-q1jx0n0l	357	19	some	some	DT
cord-104268-q1jx0n0l	357	20	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	357	21	of	of	IN
cord-104268-q1jx0n0l	357	22	the	the	DT
cord-104268-q1jx0n0l	357	23	modified	modify	VBN
cord-104268-q1jx0n0l	357	24	protein	protein	NN
cord-104268-q1jx0n0l	357	25	(	(	-LRB-
cord-104268-q1jx0n0l	357	26	Munro	Munro	NNP
cord-104268-q1jx0n0l	357	27	,	,	,
cord-104268-q1jx0n0l	357	28	1991	1991	CD
cord-104268-q1jx0n0l	357	29	;	;	:
cord-104268-q1jx0n0l	357	30	Aoki	Aoki	NNP
cord-104268-q1jx0n0l	357	31	et	et	NNP
cord-104268-q1jx0n0l	357	32	al	al	NNP
cord-104268-q1jx0n0l	357	33	.	.	NNP
cord-104268-q1jx0n0l	357	34	,	,	,
cord-104268-q1jx0n0l	357	35	1992	1992	CD
cord-104268-q1jx0n0l	357	36	;	;	:
cord-104268-q1jx0n0l	357	37	Teasdale	Teasdale	NNP
cord-104268-q1jx0n0l	357	38	et	et	NNP
cord-104268-q1jx0n0l	357	39	al	al	NNP
cord-104268-q1jx0n0l	357	40	.	.	NNP
cord-104268-q1jx0n0l	357	41	,	,	,
cord-104268-q1jx0n0l	357	42	1992	1992	CD
cord-104268-q1jx0n0l	357	43	;	;	.
cord-104268-q1jx0n0l	358	1	Burke	Burke	NNP
cord-104268-q1jx0n0l	358	2	et	et	NNP
cord-104268-q1jx0n0l	358	3	al	al	NNP
cord-104268-q1jx0n0l	358	4	.	.	.
cord-104268-q1jx0n0l	359	1	,	,	,
cord-104268-q1jx0n0l	359	2	1994	1994	CD
cord-104268-q1jx0n0l	359	3	;	;	:
cord-104268-q1jx0n0l	359	4	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	359	5	et	et	NNP
cord-104268-q1jx0n0l	359	6	al	al	NNP
cord-104268-q1jx0n0l	359	7	.	.	NNP
cord-104268-q1jx0n0l	359	8	,	,	,
cord-104268-q1jx0n0l	359	9	1994	1994	CD
cord-104268-q1jx0n0l	359	10	)	)	-RRB-
cord-104268-q1jx0n0l	359	11	.	.	.
cord-104268-q1jx0n0l	360	1	The	the	DT
cord-104268-q1jx0n0l	360	2	hydrophobic	hydrophobic	JJ
cord-104268-q1jx0n0l	360	3	TMD	TMD	NNP
cord-104268-q1jx0n0l	360	4	of	of	IN
cord-104268-q1jx0n0l	360	5	several	several	JJ
cord-104268-q1jx0n0l	360	6	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	360	7	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	360	8	appears	appear	VBZ
cord-104268-q1jx0n0l	360	9	to	to	TO
cord-104268-q1jx0n0l	360	10	be	be	VB
cord-104268-q1jx0n0l	360	11	both	both	DT
cord-104268-q1jx0n0l	360	12	necessary	necessary	JJ
cord-104268-q1jx0n0l	360	13	and	and	CC
cord-104268-q1jx0n0l	360	14	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	360	15	to	to	TO
cord-104268-q1jx0n0l	360	16	confer	confer	VB
cord-104268-q1jx0n0l	360	17	complete	complete	JJ
cord-104268-q1jx0n0l	360	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	360	19	retention	retention	NN
cord-104268-q1jx0n0l	360	20	(	(	-LRB-
cord-104268-q1jx0n0l	360	21	Nilsson	Nilsson	NNP
cord-104268-q1jx0n0l	360	22	et	et	NNP
cord-104268-q1jx0n0l	360	23	al	al	NNP
cord-104268-q1jx0n0l	360	24	.	.	NNP
cord-104268-q1jx0n0l	360	25	,	,	,
cord-104268-q1jx0n0l	360	26	1991	1991	CD
cord-104268-q1jx0n0l	360	27	;	;	:
cord-104268-q1jx0n0l	360	28	Aoki	Aoki	NNP
cord-104268-q1jx0n0l	360	29	et	et	NNP
cord-104268-q1jx0n0l	360	30	al	al	NNP
cord-104268-q1jx0n0l	360	31	.	.	NNP
cord-104268-q1jx0n0l	360	32	,	,	,
cord-104268-q1jx0n0l	360	33	1992	1992	CD
cord-104268-q1jx0n0l	360	34	;	;	:
cord-104268-q1jx0n0l	360	35	Shaper	shaper	NN
cord-104268-q1jx0n0l	360	36	and	and	CC
cord-104268-q1jx0n0l	360	37	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	360	38	,	,	,
cord-104268-q1jx0n0l	360	39	1992	1992	CD
cord-104268-q1jx0n0l	360	40	;	;	:
cord-104268-q1jx0n0l	360	41	Teasdale	Teasdale	NNP
cord-104268-q1jx0n0l	360	42	et	et	NNP
cord-104268-q1jx0n0l	360	43	al	al	NNP
cord-104268-q1jx0n0l	360	44	.	.	NNP
cord-104268-q1jx0n0l	360	45	,	,	,
cord-104268-q1jx0n0l	360	46	1992	1992	CD
cord-104268-q1jx0n0l	360	47	;	;	:
cord-104268-q1jx0n0l	360	48	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	360	49	et	et	NNP
cord-104268-q1jx0n0l	360	50	al	al	NNP
cord-104268-q1jx0n0l	360	51	.	.	NNP
cord-104268-q1jx0n0l	360	52	,	,	,
cord-104268-q1jx0n0l	360	53	1994	1994	CD
cord-104268-q1jx0n0l	360	54	;	;	:
cord-104268-q1jx0n0l	360	55	Low	Low	NNP
cord-104268-q1jx0n0l	360	56	and	and	CC
cord-104268-q1jx0n0l	360	57	Hong	Hong	NNP
cord-104268-q1jx0n0l	360	58	,	,	,
cord-104268-q1jx0n0l	360	59	1994	1994	CD
cord-104268-q1jx0n0l	360	60	)	)	-RRB-
cord-104268-q1jx0n0l	360	61	whereas	whereas	IN
cord-104268-q1jx0n0l	360	62	in	in	IN
cord-104268-q1jx0n0l	360	63	other	other	JJ
cord-104268-q1jx0n0l	360	64	cases	case	NNS
cord-104268-q1jx0n0l	360	65	,	,	,
cord-104268-q1jx0n0l	360	66	some	some	DT
cord-104268-q1jx0n0l	360	67	additional	additional	JJ
cord-104268-q1jx0n0l	360	68	flanking	flanking	JJ
cord-104268-q1jx0n0l	360	69	sequences	sequence	NNS
cord-104268-q1jx0n0l	360	70	are	be	VBP
cord-104268-q1jx0n0l	360	71	required	require	VBN
cord-104268-q1jx0n0l	360	72	to	to	TO
cord-104268-q1jx0n0l	360	73	achieve	achieve	VB
cord-104268-q1jx0n0l	360	74	effective	effective	JJ
cord-104268-q1jx0n0l	360	75	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	360	76	targeting	target	VBG
cord-104268-q1jx0n0l	360	77	(	(	-LRB-
cord-104268-q1jx0n0l	360	78	Munro	Munro	NNP
cord-104268-q1jx0n0l	360	79	,	,	,
cord-104268-q1jx0n0l	360	80	1991	1991	CD
cord-104268-q1jx0n0l	360	81	;	;	:
cord-104268-q1jx0n0l	361	1	Burke	Burke	NNP
cord-104268-q1jx0n0l	361	2	et	et	NNP
cord-104268-q1jx0n0l	361	3	al	al	NNP
cord-104268-q1jx0n0l	361	4	.	.	NNP
cord-104268-q1jx0n0l	361	5	,	,	,
cord-104268-q1jx0n0l	361	6	1992	1992	CD
cord-104268-q1jx0n0l	361	7	;	;	:
cord-104268-q1jx0n0l	361	8	Shaper	shaper	NN
cord-104268-q1jx0n0l	361	9	and	and	CC
cord-104268-q1jx0n0l	361	10	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	361	11	,	,	,
cord-104268-q1jx0n0l	361	12	1992	1992	CD
cord-104268-q1jx0n0l	362	1	;	;	:
cord-104268-q1jx0n0l	362	2	Dahdal	Dahdal	NNP
cord-104268-q1jx0n0l	362	3	et	et	FW
cord-104268-q1jx0n0l	362	4	al	al	NNP
cord-104268-q1jx0n0l	362	5	.	.	NNP
cord-104268-q1jx0n0l	362	6	,	,	,
cord-104268-q1jx0n0l	362	7	1993	1993	CD
cord-104268-q1jx0n0l	362	8	;	;	:
cord-104268-q1jx0n0l	363	1	Burke	Burke	NNP
cord-104268-q1jx0n0l	363	2	et	et	NNP
cord-104268-q1jx0n0l	363	3	al	al	NNP
cord-104268-q1jx0n0l	363	4	.	.	.
cord-104268-q1jx0n0l	364	1	,	,	,
cord-104268-q1jx0n0l	364	2	1994	1994	CD
cord-104268-q1jx0n0l	364	3	;	;	:
cord-104268-q1jx0n0l	364	4	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	364	5	et	et	NNP
cord-104268-q1jx0n0l	364	6	al	al	NNP
cord-104268-q1jx0n0l	364	7	.	.	.
cord-104268-q1jx0n0l	365	1	,	,	,
cord-104268-q1jx0n0l	365	2	1994	1994	CD
cord-104268-q1jx0n0l	365	3	;	;	:
cord-104268-q1jx0n0l	365	4	Low	Low	NNP
cord-104268-q1jx0n0l	365	5	and	and	CC
cord-104268-q1jx0n0l	365	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	365	7	,	,	,
cord-104268-q1jx0n0l	365	8	1994	1994	CD
cord-104268-q1jx0n0l	365	9	)	)	-RRB-
cord-104268-q1jx0n0l	365	10	.	.	.
cord-104268-q1jx0n0l	366	1	The	the	DT
cord-104268-q1jx0n0l	366	2	exact	exact	JJ
cord-104268-q1jx0n0l	366	3	nature	nature	NN
cord-104268-q1jx0n0l	366	4	of	of	IN
cord-104268-q1jx0n0l	366	5	these	these	DT
cord-104268-q1jx0n0l	366	6	NHz	nhz	NN
cord-104268-q1jx0n0l	366	7	-	-	HYPH
cord-104268-q1jx0n0l	366	8	terminal	terminal	NN
cord-104268-q1jx0n0l	366	9	and	and	CC
cord-104268-q1jx0n0l	366	10	stem	stem	NN
cord-104268-q1jx0n0l	366	11	sequences	sequence	NNS
cord-104268-q1jx0n0l	366	12	remains	remain	VBZ
cord-104268-q1jx0n0l	366	13	the	the	DT
cord-104268-q1jx0n0l	366	14	be	be	VB
cord-104268-q1jx0n0l	366	15	precisely	precisely	RB
cord-104268-q1jx0n0l	366	16	determined	determine	VBN
cord-104268-q1jx0n0l	366	17	but	but	CC
cord-104268-q1jx0n0l	366	18	do	do	VBP
cord-104268-q1jx0n0l	366	19	not	not	RB
cord-104268-q1jx0n0l	366	20	appear	appear	VB
cord-104268-q1jx0n0l	366	21	to	to	TO
cord-104268-q1jx0n0l	366	22	involve	involve	VB
cord-104268-q1jx0n0l	366	23	the	the	DT
cord-104268-q1jx0n0l	366	24	whole	whole	NN
cord-104268-q1jx0n0l	366	25	of	of	IN
cord-104268-q1jx0n0l	366	26	each	each	DT
cord-104268-q1jx0n0l	366	27	domain	domain	NN
cord-104268-q1jx0n0l	366	28	but	but	CC
cord-104268-q1jx0n0l	366	29	mainly	mainly	RB
cord-104268-q1jx0n0l	366	30	the	the	DT
cord-104268-q1jx0n0l	366	31	short	short	JJ
cord-104268-q1jx0n0l	366	32	stretches	stretch	NNS
cord-104268-q1jx0n0l	366	33	of	of	IN
cord-104268-q1jx0n0l	366	34	hydrophilic	hydrophilic	JJ
cord-104268-q1jx0n0l	366	35	residues	residue	NNS
cord-104268-q1jx0n0l	366	36	directly	directly	RB
cord-104268-q1jx0n0l	366	37	flanking	flank	VBG
cord-104268-q1jx0n0l	366	38	the	the	DT
cord-104268-q1jx0n0l	366	39	lipid	lipid	NN
cord-104268-q1jx0n0l	366	40	-	-	HYPH
cord-104268-q1jx0n0l	366	41	embedded	embed	VBN
cord-104268-q1jx0n0l	366	42	TMD	TMD	NNP
cord-104268-q1jx0n0l	366	43	(	(	-LRB-
cord-104268-q1jx0n0l	366	44	Munro	Munro	NNP
cord-104268-q1jx0n0l	366	45	,	,	,
cord-104268-q1jx0n0l	366	46	1991	1991	CD
cord-104268-q1jx0n0l	366	47	;	;	:
cord-104268-q1jx0n0l	366	48	Burke	Burke	NNP
cord-104268-q1jx0n0l	366	49	et	et	NNP
cord-104268-q1jx0n0l	366	50	al	al	NNP
cord-104268-q1jx0n0l	366	51	.	.	NNP
cord-104268-q1jx0n0l	366	52	,	,	,
cord-104268-q1jx0n0l	366	53	1992	1992	CD
cord-104268-q1jx0n0l	366	54	;	;	:
cord-104268-q1jx0n0l	366	55	Shaper	shaper	NN
cord-104268-q1jx0n0l	366	56	and	and	CC
cord-104268-q1jx0n0l	366	57	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	366	58	,	,	,
cord-104268-q1jx0n0l	366	59	1992	1992	CD
cord-104268-q1jx0n0l	366	60	;	;	:
cord-104268-q1jx0n0l	366	61	Dahdal	Dahdal	NNP
cord-104268-q1jx0n0l	366	62	et	et	FW
cord-104268-q1jx0n0l	366	63	al	al	NNP
cord-104268-q1jx0n0l	366	64	.	.	NNP
cord-104268-q1jx0n0l	366	65	,	,	,
cord-104268-q1jx0n0l	366	66	1993	1993	CD
cord-104268-q1jx0n0l	366	67	;	;	:
cord-104268-q1jx0n0l	367	1	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	367	2	et	et	NNP
cord-104268-q1jx0n0l	367	3	al	al	NNP
cord-104268-q1jx0n0l	367	4	.	.	.
cord-104268-q1jx0n0l	368	1	,	,	,
cord-104268-q1jx0n0l	368	2	1994	1994	CD
cord-104268-q1jx0n0l	368	3	;	;	:
cord-104268-q1jx0n0l	368	4	Low	Low	NNP
cord-104268-q1jx0n0l	368	5	and	and	CC
cord-104268-q1jx0n0l	368	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	368	7	,	,	,
cord-104268-q1jx0n0l	368	8	1994	1994	CD
cord-104268-q1jx0n0l	368	9	)	)	-RRB-
cord-104268-q1jx0n0l	368	10	.	.	.
cord-104268-q1jx0n0l	369	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	369	2	has	have	VBZ
cord-104268-q1jx0n0l	369	3	been	be	VBN
cord-104268-q1jx0n0l	369	4	postulated	postulate	VBN
cord-104268-q1jx0n0l	369	5	that	that	IN
cord-104268-q1jx0n0l	369	6	the	the	DT
cord-104268-q1jx0n0l	369	7	targeting	targeting	NN
cord-104268-q1jx0n0l	369	8	of	of	IN
cord-104268-q1jx0n0l	369	9	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	369	10	carrying	carry	VBG
cord-104268-q1jx0n0l	369	11	a	a	DT
cord-104268-q1jx0n0l	369	12	TMD	TMD	NNP
cord-104268-q1jx0n0l	369	13	sorting	sorting	NN
cord-104268-q1jx0n0l	369	14	signal	signal	NN
cord-104268-q1jx0n0l	369	15	could	could	MD
cord-104268-q1jx0n0l	369	16	be	be	VB
cord-104268-q1jx0n0l	369	17	due	due	JJ
cord-104268-q1jx0n0l	369	18	to	to	IN
cord-104268-q1jx0n0l	369	19	interactions	interaction	NNS
cord-104268-q1jx0n0l	369	20	between	between	IN
cord-104268-q1jx0n0l	369	21	the	the	DT
cord-104268-q1jx0n0l	369	22	membrane	membrane	NN
cord-104268-q1jx0n0l	369	23	-	-	HYPH
cord-104268-q1jx0n0l	369	24	spanning	span	VBG
cord-104268-q1jx0n0l	369	25	domain	domain	NN
cord-104268-q1jx0n0l	369	26	and	and	CC
cord-104268-q1jx0n0l	369	27	compartment	compartment	NN
cord-104268-q1jx0n0l	369	28	specific	specific	JJ
cord-104268-q1jx0n0l	369	29	membrane	membrane	NN
cord-104268-q1jx0n0l	369	30	lipids	lipid	NNS
cord-104268-q1jx0n0l	369	31	(	(	-LRB-
cord-104268-q1jx0n0l	369	32	Pelham	Pelham	NNP
cord-104268-q1jx0n0l	369	33	and	and	CC
cord-104268-q1jx0n0l	369	34	Munro	Munro	NNP
cord-104268-q1jx0n0l	369	35	,	,	,
cord-104268-q1jx0n0l	369	36	1993	1993	CD
cord-104268-q1jx0n0l	369	37	)	)	-RRB-
cord-104268-q1jx0n0l	369	38	.	.	.
cord-104268-q1jx0n0l	370	1	There	there	EX
cord-104268-q1jx0n0l	370	2	is	be	VBZ
cord-104268-q1jx0n0l	370	3	no	no	DT
cord-104268-q1jx0n0l	370	4	evidence	evidence	NN
cord-104268-q1jx0n0l	370	5	that	that	IN
cord-104268-q1jx0n0l	370	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	370	7	is	be	VBZ
cord-104268-q1jx0n0l	370	8	retained	retain	VBN
cord-104268-q1jx0n0l	370	9	by	by	IN
cord-104268-q1jx0n0l	370	10	such	such	PDT
cord-104268-q1jx0n0l	370	11	a	a	DT
cord-104268-q1jx0n0l	370	12	mechanism	mechanism	NN
cord-104268-q1jx0n0l	370	13	,	,	,
cord-104268-q1jx0n0l	370	14	since	since	IN
cord-104268-q1jx0n0l	370	15	in	in	IN
cord-104268-q1jx0n0l	370	16	the	the	DT
cord-104268-q1jx0n0l	370	17	series	series	NN
cord-104268-q1jx0n0l	370	18	of	of	IN
cord-104268-q1jx0n0l	370	19	constructs	construct	NNS
cord-104268-q1jx0n0l	370	20	we	-PRON-	PRP
cord-104268-q1jx0n0l	370	21	have	have	VBP
cord-104268-q1jx0n0l	370	22	devised	devise	VBN
cord-104268-q1jx0n0l	370	23	,	,	,
cord-104268-q1jx0n0l	370	24	the	the	DT
cord-104268-q1jx0n0l	370	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	370	26	TMD	TMD	NNP
cord-104268-q1jx0n0l	370	27	is	be	VBZ
cord-104268-q1jx0n0l	370	28	not	not	RB
cord-104268-q1jx0n0l	370	29	implicated	implicate	VBN
cord-104268-q1jx0n0l	370	30	in	in	IN
cord-104268-q1jx0n0l	370	31	retention	retention	NN
cord-104268-q1jx0n0l	370	32	.	.	.
cord-104268-q1jx0n0l	371	1	In	in	IN
cord-104268-q1jx0n0l	371	2	this	this	DT
cord-104268-q1jx0n0l	371	3	lipid	lipid	NN
cord-104268-q1jx0n0l	371	4	interaction	interaction	NN
cord-104268-q1jx0n0l	371	5	model	model	NN
cord-104268-q1jx0n0l	371	6	,	,	,
cord-104268-q1jx0n0l	371	7	the	the	DT
cord-104268-q1jx0n0l	371	8	length	length	NN
cord-104268-q1jx0n0l	371	9	of	of	IN
cord-104268-q1jx0n0l	371	10	the	the	DT
cord-104268-q1jx0n0l	371	11	membranespanning	membranespanne	VBG
cord-104268-q1jx0n0l	371	12	domain	domain	NN
cord-104268-q1jx0n0l	371	13	is	be	VBZ
cord-104268-q1jx0n0l	371	14	important	important	JJ
cord-104268-q1jx0n0l	371	15	for	for	IN
cord-104268-q1jx0n0l	371	16	the	the	DT
cord-104268-q1jx0n0l	371	17	proper	proper	JJ
cord-104268-q1jx0n0l	371	18	sorting	sorting	NN
cord-104268-q1jx0n0l	371	19	of	of	IN
cord-104268-q1jx0n0l	371	20	animal	animal	NN
cord-104268-q1jx0n0l	371	21	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	371	22	(	(	-LRB-
cord-104268-q1jx0n0l	371	23	Munro	Munro	NNP
cord-104268-q1jx0n0l	371	24	,	,	,
cord-104268-q1jx0n0l	371	25	1991	1991	CD
cord-104268-q1jx0n0l	371	26	;	;	:
cord-104268-q1jx0n0l	371	27	Pelham	Pelham	NNP
cord-104268-q1jx0n0l	371	28	and	and	CC
cord-104268-q1jx0n0l	371	29	Munro	Munro	NNP
cord-104268-q1jx0n0l	371	30	,	,	,
cord-104268-q1jx0n0l	371	31	1993	1993	CD
cord-104268-q1jx0n0l	371	32	)	)	-RRB-
cord-104268-q1jx0n0l	371	33	.	.	.
cord-104268-q1jx0n0l	372	1	In	in	IN
cord-104268-q1jx0n0l	372	2	the	the	DT
cord-104268-q1jx0n0l	372	3	case	case	NN
cord-104268-q1jx0n0l	372	4	of	of	IN
cord-104268-q1jx0n0l	372	5	KDKK	KDKK	NNP
cord-104268-q1jx0n0l	373	1	the	the	DT
cord-104268-q1jx0n0l	373	2	TMD	TMD	NNP
cord-104268-q1jx0n0l	373	3	of	of	IN
cord-104268-q1jx0n0l	373	4	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	373	5	B	B	NNP
cord-104268-q1jx0n0l	373	6	is	be	VBZ
cord-104268-q1jx0n0l	373	7	three	three	CD
cord-104268-q1jx0n0l	373	8	amino	amino	NN
cord-104268-q1jx0n0l	373	9	acid	acid	NN
cord-104268-q1jx0n0l	373	10	residues	residue	NNS
cord-104268-q1jx0n0l	373	11	shorter	short	JJR
cord-104268-q1jx0n0l	373	12	than	than	IN
cord-104268-q1jx0n0l	373	13	the	the	DT
cord-104268-q1jx0n0l	373	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	373	15	TMD	TMD	NNP
cord-104268-q1jx0n0l	373	16	,	,	,
cord-104268-q1jx0n0l	373	17	and	and	CC
cord-104268-q1jx0n0l	373	18	in	in	IN
cord-104268-q1jx0n0l	373	19	the	the	DT
cord-104268-q1jx0n0l	373	20	case	case	NN
cord-104268-q1jx0n0l	373	21	of	of	IN
cord-104268-q1jx0n0l	373	22	KPKK	KPKK	NNP
cord-104268-q1jx0n0l	373	23	the	the	DT
cord-104268-q1jx0n0l	373	24	TMD	TMD	NNP
cord-104268-q1jx0n0l	373	25	of	of	IN
cord-104268-q1jx0n0l	373	26	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	373	27	is	be	VBZ
cord-104268-q1jx0n0l	373	28	seven	seven	CD
cord-104268-q1jx0n0l	373	29	amino	amino	NN
cord-104268-q1jx0n0l	373	30	acid	acid	NN
cord-104268-q1jx0n0l	373	31	residues	residue	NNS
cord-104268-q1jx0n0l	373	32	longer	long	RBR
cord-104268-q1jx0n0l	373	33	than	than	IN
cord-104268-q1jx0n0l	373	34	the	the	DT
cord-104268-q1jx0n0l	373	35	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	373	36	TMD	TMD	NNP
cord-104268-q1jx0n0l	373	37	,	,	,
cord-104268-q1jx0n0l	373	38	yet	yet	CC
cord-104268-q1jx0n0l	373	39	both	both	DT
cord-104268-q1jx0n0l	373	40	are	be	VBP
cord-104268-q1jx0n0l	373	41	retained	retain	VBN
cord-104268-q1jx0n0l	373	42	in	in	IN
cord-104268-q1jx0n0l	373	43	the	the	DT
cord-104268-q1jx0n0l	373	44	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	373	45	complex	complex	NN
cord-104268-q1jx0n0l	373	46	.	.	.
cord-104268-q1jx0n0l	374	1	None	none	NN
cord-104268-q1jx0n0l	374	2	of	of	IN
cord-104268-q1jx0n0l	374	3	the	the	DT
cord-104268-q1jx0n0l	374	4	studied	study	VBN
cord-104268-q1jx0n0l	374	5	animal	animal	NN
cord-104268-q1jx0n0l	374	6	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	374	7	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	374	8	appear	appear	VBP
cord-104268-q1jx0n0l	374	9	to	to	TO
cord-104268-q1jx0n0l	374	10	need	need	VB
cord-104268-q1jx0n0l	374	11	an	an	DT
cord-104268-q1jx0n0l	374	12	intact	intact	JJ
cord-104268-q1jx0n0l	374	13	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	374	14	domain	domain	NN
cord-104268-q1jx0n0l	374	15	to	to	TO
cord-104268-q1jx0n0l	374	16	achieve	achieve	VB
cord-104268-q1jx0n0l	374	17	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	374	18	retention	retention	NN
cord-104268-q1jx0n0l	374	19	(	(	-LRB-
cord-104268-q1jx0n0l	374	20	Munro	Munro	NNP
cord-104268-q1jx0n0l	374	21	,	,	,
cord-104268-q1jx0n0l	374	22	1991	1991	CD
cord-104268-q1jx0n0l	374	23	;	;	:
cord-104268-q1jx0n0l	374	24	Aoki	Aoki	NNP
cord-104268-q1jx0n0l	374	25	et	et	NNP
cord-104268-q1jx0n0l	374	26	al	al	NNP
cord-104268-q1jx0n0l	374	27	.	.	NNP
cord-104268-q1jx0n0l	374	28	,	,	,
cord-104268-q1jx0n0l	374	29	1992	1992	CD
cord-104268-q1jx0n0l	374	30	;	;	:
cord-104268-q1jx0n0l	374	31	Colley	Colley	NNP
cord-104268-q1jx0n0l	374	32	et	et	NNP
cord-104268-q1jx0n0l	374	33	al	al	NNP
cord-104268-q1jx0n0l	374	34	.	.	NNP
cord-104268-q1jx0n0l	374	35	,	,	,
cord-104268-q1jx0n0l	374	36	1992	1992	CD
cord-104268-q1jx0n0l	374	37	;	;	:
cord-104268-q1jx0n0l	374	38	Shaper	shaper	NN
cord-104268-q1jx0n0l	374	39	and	and	CC
cord-104268-q1jx0n0l	374	40	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	374	41	,	,	,
cord-104268-q1jx0n0l	374	42	1992	1992	CD
cord-104268-q1jx0n0l	374	43	;	;	:
cord-104268-q1jx0n0l	374	44	Teasdale	Teasdale	NNP
cord-104268-q1jx0n0l	374	45	et	et	NNP
cord-104268-q1jx0n0l	374	46	al	al	NNP
cord-104268-q1jx0n0l	374	47	.	.	.
cord-104268-q1jx0n0l	375	1	,	,	,
cord-104268-q1jx0n0l	375	2	1992	1992	CD
cord-104268-q1jx0n0l	375	3	;	;	:
cord-104268-q1jx0n0l	375	4	Dahdal	Dahdal	NNP
cord-104268-q1jx0n0l	375	5	et	et	FW
cord-104268-q1jx0n0l	375	6	al	al	NNP
cord-104268-q1jx0n0l	375	7	.	.	NNP
cord-104268-q1jx0n0l	375	8	,	,	,
cord-104268-q1jx0n0l	375	9	1993	1993	CD
cord-104268-q1jx0n0l	375	10	;	;	:
cord-104268-q1jx0n0l	376	1	Burke	Burke	NNP
cord-104268-q1jx0n0l	376	2	et	et	NNP
cord-104268-q1jx0n0l	376	3	al	al	NNP
cord-104268-q1jx0n0l	376	4	.	.	.
cord-104268-q1jx0n0l	377	1	,	,	,
cord-104268-q1jx0n0l	377	2	1994	1994	CD
cord-104268-q1jx0n0l	377	3	;	;	:
cord-104268-q1jx0n0l	377	4	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	377	5	et	et	NNP
cord-104268-q1jx0n0l	377	6	al	al	NNP
cord-104268-q1jx0n0l	377	7	.	.	.
cord-104268-q1jx0n0l	378	1	,	,	,
cord-104268-q1jx0n0l	378	2	1994	1994	CD
cord-104268-q1jx0n0l	378	3	;	;	:
cord-104268-q1jx0n0l	378	4	Low	Low	NNP
cord-104268-q1jx0n0l	378	5	and	and	CC
cord-104268-q1jx0n0l	378	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	378	7	,	,	,
cord-104268-q1jx0n0l	378	8	1994	1994	CD
cord-104268-q1jx0n0l	378	9	)	)	-RRB-
cord-104268-q1jx0n0l	378	10	.	.	.
cord-104268-q1jx0n0l	379	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	379	2	,	,	,
cord-104268-q1jx0n0l	379	3	thus	thus	RB
cord-104268-q1jx0n0l	379	4	,	,	,
cord-104268-q1jx0n0l	379	5	constitutes	constitute	VBZ
cord-104268-q1jx0n0l	379	6	the	the	DT
cord-104268-q1jx0n0l	379	7	first	first	JJ
cord-104268-q1jx0n0l	379	8	demonstration	demonstration	NN
cord-104268-q1jx0n0l	379	9	of	of	IN
cord-104268-q1jx0n0l	379	10	an	an	DT
cord-104268-q1jx0n0l	379	11	eukaryotic	eukaryotic	JJ
cord-104268-q1jx0n0l	379	12	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	379	13	requiring	require	VBG
cord-104268-q1jx0n0l	379	14	a	a	DT
cord-104268-q1jx0n0l	379	15	short	short	JJ
cord-104268-q1jx0n0l	379	16	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	379	17	amino	amino	NN
cord-104268-q1jx0n0l	379	18	-	-	HYPH
cord-104268-q1jx0n0l	379	19	terminal	terminal	NN
cord-104268-q1jx0n0l	379	20	domain	domain	NN
cord-104268-q1jx0n0l	379	21	for	for	IN
cord-104268-q1jx0n0l	379	22	correct	correct	JJ
cord-104268-q1jx0n0l	379	23	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	379	24	localization	localization	NN
cord-104268-q1jx0n0l	379	25	.	.	.
cord-104268-q1jx0n0l	380	1	The	the	DT
cord-104268-q1jx0n0l	380	2	11-amino	11-amino	CD
cord-104268-q1jx0n0l	380	3	acid	acid	NN
cord-104268-q1jx0n0l	380	4	residue	residue	NN
cord-104268-q1jx0n0l	380	5	-	-	HYPH
cord-104268-q1jx0n0l	380	6	targeting	target	VBG
cord-104268-q1jx0n0l	380	7	domain	domain	NN
cord-104268-q1jx0n0l	380	8	of	of	IN
cord-104268-q1jx0n0l	380	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	380	10	seemingly	seemingly	RB
cord-104268-q1jx0n0l	380	11	parallels	parallel	VBZ
cord-104268-q1jx0n0l	380	12	that	that	DT
cord-104268-q1jx0n0l	380	13	of	of	IN
cord-104268-q1jx0n0l	380	14	the	the	DT
cord-104268-q1jx0n0l	380	15	late	late	JJ
cord-104268-q1jx0n0l	380	16	yeast	yeast	NN
cord-104268-q1jx0n0l	380	17	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	380	18	enzymes	enzyme	VBZ
cord-104268-q1jx0n0l	380	19	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	380	20	,	,	,
cord-104268-q1jx0n0l	380	21	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	380	22	and	and	CC
cord-104268-q1jx0n0l	380	23	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	380	24	A	A	NNP
cord-104268-q1jx0n0l	380	25	(	(	-LRB-
cord-104268-q1jx0n0l	380	26	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	380	27	and	and	CC
cord-104268-q1jx0n0l	380	28	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	380	29	,	,	,
cord-104268-q1jx0n0l	380	30	1992	1992	CD
cord-104268-q1jx0n0l	380	31	;	;	:
cord-104268-q1jx0n0l	380	32	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	380	33	et	et	NNP
cord-104268-q1jx0n0l	380	34	al	al	NNP
cord-104268-q1jx0n0l	380	35	.	.	.
cord-104268-q1jx0n0l	381	1	,	,	,
cord-104268-q1jx0n0l	381	2	1992	1992	CD
cord-104268-q1jx0n0l	381	3	;	;	:
cord-104268-q1jx0n0l	381	4	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	381	5	et	et	NNP
cord-104268-q1jx0n0l	381	6	al	al	NNP
cord-104268-q1jx0n0l	381	7	.	.	NNP
cord-104268-q1jx0n0l	381	8	,	,	,
cord-104268-q1jx0n0l	381	9	1993	1993	CD
cord-104268-q1jx0n0l	381	10	)	)	-RRB-
cord-104268-q1jx0n0l	381	11	.	.	.
cord-104268-q1jx0n0l	382	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	382	2	was	be	VBD
cord-104268-q1jx0n0l	382	3	shown	show	VBN
cord-104268-q1jx0n0l	382	4	for	for	IN
cord-104268-q1jx0n0l	382	5	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	382	6	A	A	NNP
cord-104268-q1jx0n0l	382	7	that	that	WDT
cord-104268-q1jx0n0l	382	8	a	a	DT
cord-104268-q1jx0n0l	382	9	10-amino	10-amino	CD
cord-104268-q1jx0n0l	382	10	acid	acid	NN
cord-104268-q1jx0n0l	382	11	region	region	NN
cord-104268-q1jx0n0l	382	12	within	within	IN
cord-104268-q1jx0n0l	382	13	the	the	DT
cord-104268-q1jx0n0l	382	14	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	382	15	domain	domain	NN
cord-104268-q1jx0n0l	382	16	was	be	VBD
cord-104268-q1jx0n0l	382	17	both	both	DT
cord-104268-q1jx0n0l	382	18	required	required	JJ
cord-104268-q1jx0n0l	382	19	and	and	CC
cord-104268-q1jx0n0l	382	20	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	382	21	for	for	IN
cord-104268-q1jx0n0l	382	22	proper	proper	JJ
cord-104268-q1jx0n0l	382	23	retention	retention	NN
cord-104268-q1jx0n0l	383	1	(	(	-LRB-
cord-104268-q1jx0n0l	383	2	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	383	3	et	et	NNP
cord-104268-q1jx0n0l	383	4	al	al	NNP
cord-104268-q1jx0n0l	383	5	.	.	NNP
cord-104268-q1jx0n0l	383	6	,	,	,
cord-104268-q1jx0n0l	383	7	1993	1993	CD
cord-104268-q1jx0n0l	383	8	)	)	-RRB-
cord-104268-q1jx0n0l	383	9	,	,	,
cord-104268-q1jx0n0l	383	10	and	and	CC
cord-104268-q1jx0n0l	383	11	for	for	IN
cord-104268-q1jx0n0l	383	12	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	383	13	,	,	,
cord-104268-q1jx0n0l	383	14	a	a	DT
cord-104268-q1jx0n0l	383	15	27-amino	27-amino	CD
cord-104268-q1jx0n0l	383	16	acid	acid	NN
cord-104268-q1jx0n0l	383	17	region	region	NN
cord-104268-q1jx0n0l	383	18	was	be	VBD
cord-104268-q1jx0n0l	383	19	found	find	VBN
cord-104268-q1jx0n0l	383	20	to	to	TO
cord-104268-q1jx0n0l	383	21	be	be	VB
cord-104268-q1jx0n0l	383	22	essential	essential	JJ
cord-104268-q1jx0n0l	383	23	(	(	-LRB-
cord-104268-q1jx0n0l	384	1	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	384	2	et	et	NNP
cord-104268-q1jx0n0l	384	3	al	al	NNP
cord-104268-q1jx0n0l	384	4	.	.	NNP
cord-104268-q1jx0n0l	384	5	,	,	,
cord-104268-q1jx0n0l	384	6	1992	1992	CD
cord-104268-q1jx0n0l	384	7	)	)	-RRB-
cord-104268-q1jx0n0l	384	8	.	.	.
cord-104268-q1jx0n0l	385	1	The	the	DT
cord-104268-q1jx0n0l	385	2	accurate	accurate	JJ
cord-104268-q1jx0n0l	385	3	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	385	4	targeting	targeting	NN
cord-104268-q1jx0n0l	385	5	of	of	IN
cord-104268-q1jx0n0l	385	6	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	385	7	and	and	CC
cord-104268-q1jx0n0l	385	8	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	385	9	A	A	NNP
cord-104268-q1jx0n0l	385	10	is	be	VBZ
cord-104268-q1jx0n0l	386	1	clathrin	clathrin	NNP
cord-104268-q1jx0n0l	386	2	dependent	dependent	JJ
cord-104268-q1jx0n0l	386	3	and	and	CC
cord-104268-q1jx0n0l	386	4	aromatic	aromatic	JJ
cord-104268-q1jx0n0l	386	5	residues	residue	NNS
cord-104268-q1jx0n0l	386	6	are	be	VBP
cord-104268-q1jx0n0l	386	7	thought	think	VBN
cord-104268-q1jx0n0l	386	8	necessary	necessary	JJ
cord-104268-q1jx0n0l	386	9	for	for	IN
cord-104268-q1jx0n0l	386	10	this	this	DT
cord-104268-q1jx0n0l	386	11	retention	retention	NN
cord-104268-q1jx0n0l	386	12	process	process	NN
cord-104268-q1jx0n0l	386	13	(	(	-LRB-
cord-104268-q1jx0n0l	386	14	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	386	15	et	et	NNP
cord-104268-q1jx0n0l	386	16	al	al	NNP
cord-104268-q1jx0n0l	386	17	.	.	NNP
cord-104268-q1jx0n0l	386	18	,	,	,
cord-104268-q1jx0n0l	386	19	1992	1992	CD
cord-104268-q1jx0n0l	386	20	;	;	:
cord-104268-q1jx0n0l	386	21	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	386	22	et	et	FW
cord-104268-q1jx0n0l	386	23	al	al	NNP
cord-104268-q1jx0n0l	386	24	.	.	.
cord-104268-q1jx0n0l	387	1	,	,	,
cord-104268-q1jx0n0l	387	2	1993	1993	CD
cord-104268-q1jx0n0l	387	3	;	;	:
cord-104268-q1jx0n0l	387	4	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	387	5	and	and	CC
cord-104268-q1jx0n0l	387	6	Payne	Payne	NNP
cord-104268-q1jx0n0l	387	7	,	,	,
cord-104268-q1jx0n0l	387	8	1993	1993	CD
cord-104268-q1jx0n0l	387	9	)	)	-RRB-
cord-104268-q1jx0n0l	387	10	.	.	.
cord-104268-q1jx0n0l	388	1	While	while	IN
cord-104268-q1jx0n0l	388	2	unrelated	unrelated	JJ
cord-104268-q1jx0n0l	388	3	at	at	IN
cord-104268-q1jx0n0l	388	4	a	a	DT
cord-104268-q1jx0n0l	388	5	sequence	sequence	NN
cord-104268-q1jx0n0l	388	6	level	level	NN
cord-104268-q1jx0n0l	388	7	with	with	IN
cord-104268-q1jx0n0l	388	8	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	388	9	and	and	CC
cord-104268-q1jx0n0l	388	10	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	388	11	A	A	NNP
cord-104268-q1jx0n0l	388	12	,	,	,
cord-104268-q1jx0n0l	388	13	the	the	DT
cord-104268-q1jx0n0l	388	14	amino	amino	NN
cord-104268-q1jx0n0l	388	15	-	-	HYPH
cord-104268-q1jx0n0l	388	16	terminal	terminal	NN
cord-104268-q1jx0n0l	388	17	domain	domain	NN
cord-104268-q1jx0n0l	388	18	of	of	IN
cord-104268-q1jx0n0l	388	19	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	388	20	contains	contain	VBZ
cord-104268-q1jx0n0l	388	21	a	a	DT
cord-104268-q1jx0n0l	388	22	single	single	JJ
cord-104268-q1jx0n0l	388	23	aromatic	aromatic	JJ
cord-104268-q1jx0n0l	388	24	phenylalanine	phenylalanine	NN
cord-104268-q1jx0n0l	388	25	residue	residue	NN
cord-104268-q1jx0n0l	388	26	(	(	-LRB-
cord-104268-q1jx0n0l	388	27	MALFLS	MALFLS	NNP
cord-104268-q1jx0n0l	388	28	-	-	HYPH
cord-104268-q1jx0n0l	388	29	KRLLR	KRLLR	NNP
cord-104268-q1jx0n0l	388	30	)	)	-RRB-
cord-104268-q1jx0n0l	388	31	which	which	WDT
cord-104268-q1jx0n0l	388	32	was	be	VBD
cord-104268-q1jx0n0l	388	33	mutated	mutate	VBN
cord-104268-q1jx0n0l	388	34	to	to	IN
cord-104268-q1jx0n0l	388	35	an	an	DT
cord-104268-q1jx0n0l	388	36	alanine	alanine	NN
cord-104268-q1jx0n0l	388	37	,	,	,
cord-104268-q1jx0n0l	388	38	an	an	DT
cord-104268-q1jx0n0l	388	39	alteration	alteration	NN
cord-104268-q1jx0n0l	388	40	showing	show	VBG
cord-104268-q1jx0n0l	388	41	no	no	DT
cord-104268-q1jx0n0l	388	42	effect	effect	NN
cord-104268-q1jx0n0l	388	43	on	on	IN
cord-104268-q1jx0n0l	388	44	targeting	targeting	NN
cord-104268-q1jx0n0l	388	45	and	and	CC
cord-104268-q1jx0n0l	388	46	,	,	,
cord-104268-q1jx0n0l	388	47	therefore	therefore	RB
cord-104268-q1jx0n0l	388	48	,	,	,
cord-104268-q1jx0n0l	388	49	providing	provide	VBG
cord-104268-q1jx0n0l	388	50	no	no	DT
cord-104268-q1jx0n0l	388	51	evidence	evidence	NN
cord-104268-q1jx0n0l	388	52	for	for	IN
cord-104268-q1jx0n0l	388	53	clathrin	clathrin	NN
cord-104268-q1jx0n0l	388	54	-	-	HYPH
cord-104268-q1jx0n0l	388	55	based	base	VBN
cord-104268-q1jx0n0l	388	56	retention	retention	NN
cord-104268-q1jx0n0l	388	57	of	of	IN
cord-104268-q1jx0n0l	388	58	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	389	1	(	(	-LRB-
cord-104268-q1jx0n0l	389	2	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	389	3	,	,	,
cord-104268-q1jx0n0l	389	4	M.	M.	NNP
cord-104268-q1jx0n0l	389	5	,	,	,
cord-104268-q1jx0n0l	389	6	A.-M.	A.-M.	NNP
cord-104268-q1jx0n0l	389	7	Sdicu	Sdicu	NNP
cord-104268-q1jx0n0l	389	8	,	,	,
cord-104268-q1jx0n0l	389	9	T.	T.	NNP
cord-104268-q1jx0n0l	389	10	Ketela	Ketela	NNP
cord-104268-q1jx0n0l	389	11	,	,	,
cord-104268-q1jx0n0l	389	12	and	and	CC
cord-104268-q1jx0n0l	389	13	H.	H.	NNP
cord-104268-q1jx0n0l	389	14	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	389	15	,	,	,
cord-104268-q1jx0n0l	389	16	unpublished	unpublished	JJ
cord-104268-q1jx0n0l	389	17	results	result	NNS
cord-104268-q1jx0n0l	389	18	)	)	-RRB-
cord-104268-q1jx0n0l	389	19	.	.	.
cord-104268-q1jx0n0l	390	1	The	the	DT
cord-104268-q1jx0n0l	390	2	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	390	3	al,3-mannosyltransferase	al,3-mannosyltransferase	NNP
cord-104268-q1jx0n0l	390	4	is	be	VBZ
cord-104268-q1jx0n0l	390	5	the	the	DT
cord-104268-q1jx0n0l	390	6	only	only	JJ
cord-104268-q1jx0n0l	390	7	other	other	JJ
cord-104268-q1jx0n0l	390	8	yeast	yeast	NN
cord-104268-q1jx0n0l	390	9	glycosyltransferase	glycosyltransferase	NN
cord-104268-q1jx0n0l	390	10	where	where	WRB
cord-104268-q1jx0n0l	390	11	targeting	targeting	NN
cord-104268-q1jx0n0l	390	12	has	have	VBZ
cord-104268-q1jx0n0l	390	13	been	be	VBN
cord-104268-q1jx0n0l	390	14	examined	examine	VBN
cord-104268-q1jx0n0l	390	15	.	.	.
cord-104268-q1jx0n0l	391	1	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	391	2	targeting	targeting	NN
cord-104268-q1jx0n0l	391	3	appears	appear	VBZ
cord-104268-q1jx0n0l	391	4	different	different	JJ
cord-104268-q1jx0n0l	391	5	from	from	IN
cord-104268-q1jx0n0l	391	6	that	that	DT
cord-104268-q1jx0n0l	391	7	of	of	IN
cord-104268-q1jx0n0l	391	8	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	391	9	since	since	IN
cord-104268-q1jx0n0l	391	10	a	a	DT
cord-104268-q1jx0n0l	391	11	mutant	mutant	JJ
cord-104268-q1jx0n0l	391	12	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	391	13	lacking	lack	VBG
cord-104268-q1jx0n0l	391	14	its	-PRON-	PRP$
cord-104268-q1jx0n0l	391	15	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	391	16	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	391	17	tail	tail	NN
cord-104268-q1jx0n0l	391	18	is	be	VBZ
cord-104268-q1jx0n0l	391	19	properly	properly	RB
cord-104268-q1jx0n0l	391	20	localized	localize	VBN
cord-104268-q1jx0n0l	391	21	to	to	IN
cord-104268-q1jx0n0l	391	22	the	the	DT
cord-104268-q1jx0n0l	391	23	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	391	24	complex	complex	NN
cord-104268-q1jx0n0l	391	25	.	.	.
cord-104268-q1jx0n0l	392	1	Correct	correct	JJ
cord-104268-q1jx0n0l	392	2	retention	retention	NN
cord-104268-q1jx0n0l	392	3	of	of	IN
cord-104268-q1jx0n0l	392	4	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	392	5	is	be	VBZ
cord-104268-q1jx0n0l	392	6	clathrin	clathrin	NN
cord-104268-q1jx0n0l	392	7	dependent	dependent	JJ
cord-104268-q1jx0n0l	392	8	but	but	CC
cord-104268-q1jx0n0l	392	9	,	,	,
cord-104268-q1jx0n0l	392	10	contrary	contrary	JJ
cord-104268-q1jx0n0l	392	11	to	to	IN
cord-104268-q1jx0n0l	392	12	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	392	13	and	and	CC
cord-104268-q1jx0n0l	392	14	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	392	15	A	A	NNP
cord-104268-q1jx0n0l	392	16	,	,	,
cord-104268-q1jx0n0l	392	17	does	do	VBZ
cord-104268-q1jx0n0l	392	18	not	not	RB
cord-104268-q1jx0n0l	392	19	seem	seem	VB
cord-104268-q1jx0n0l	392	20	to	to	TO
cord-104268-q1jx0n0l	392	21	be	be	VB
cord-104268-q1jx0n0l	392	22	mediated	mediate	VBN
cord-104268-q1jx0n0l	392	23	by	by	IN
cord-104268-q1jx0n0l	392	24	a	a	DT
cord-104268-q1jx0n0l	392	25	direct	direct	JJ
cord-104268-q1jx0n0l	392	26	interaction	interaction	NN
cord-104268-q1jx0n0l	392	27	through	through	IN
cord-104268-q1jx0n0l	392	28	its	-PRON-	PRP$
cord-104268-q1jx0n0l	392	29	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	392	30	tail	tail	NN
cord-104268-q1jx0n0l	392	31	.	.	.
cord-104268-q1jx0n0l	393	1	The	the	DT
cord-104268-q1jx0n0l	393	2	reason	reason	NN
cord-104268-q1jx0n0l	393	3	for	for	IN
cord-104268-q1jx0n0l	393	4	the	the	DT
cord-104268-q1jx0n0l	393	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	393	6	requirement	requirement	NN
cord-104268-q1jx0n0l	393	7	for	for	IN
cord-104268-q1jx0n0l	393	8	its	-PRON-	PRP$
cord-104268-q1jx0n0l	393	9	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	393	10	tail	tail	NN
cord-104268-q1jx0n0l	393	11	remains	remain	VBZ
cord-104268-q1jx0n0l	393	12	unclear	unclear	JJ
cord-104268-q1jx0n0l	393	13	.	.	.
cord-104268-q1jx0n0l	394	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	394	2	could	could	MD
cord-104268-q1jx0n0l	394	3	be	be	VB
cord-104268-q1jx0n0l	394	4	clathrin	clathrin	NN
cord-104268-q1jx0n0l	394	5	dependent	dependent	JJ
cord-104268-q1jx0n0l	394	6	but	but	CC
cord-104268-q1jx0n0l	394	7	not	not	RB
cord-104268-q1jx0n0l	394	8	involving	involve	VBG
cord-104268-q1jx0n0l	394	9	an	an	DT
cord-104268-q1jx0n0l	394	10	aromatic	aromatic	JJ
cord-104268-q1jx0n0l	394	11	residue	residue	NN
cord-104268-q1jx0n0l	394	12	,	,	,
cord-104268-q1jx0n0l	394	13	or	or	CC
cord-104268-q1jx0n0l	394	14	through	through	IN
cord-104268-q1jx0n0l	394	15	some	some	DT
cord-104268-q1jx0n0l	394	16	other	other	JJ
cord-104268-q1jx0n0l	394	17	process	process	NN
cord-104268-q1jx0n0l	394	18	.	.	.
cord-104268-q1jx0n0l	395	1	The	the	DT
cord-104268-q1jx0n0l	395	2	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	395	3	type	type	NN
cord-104268-q1jx0n0l	395	4	II	II	NNP
cord-104268-q1jx0n0l	395	5	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	395	6	membrane	membrane	NN
cord-104268-q1jx0n0l	395	7	protein	protein	NN
cord-104268-q1jx0n0l	395	8	(	(	-LRB-
cord-104268-q1jx0n0l	395	9	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	395	10	et	et	NNP
cord-104268-q1jx0n0l	395	11	al	al	NNP
cord-104268-q1jx0n0l	395	12	.	.	NNP
cord-104268-q1jx0n0l	395	13	,	,	,
cord-104268-q1jx0n0l	395	14	1989	1989	CD
cord-104268-q1jx0n0l	395	15	;	;	:
cord-104268-q1jx0n0l	395	16	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	395	17	el	el	NNP
cord-104268-q1jx0n0l	395	18	al	al	NNP
cord-104268-q1jx0n0l	395	19	.	.	NNP
cord-104268-q1jx0n0l	395	20	,	,	,
cord-104268-q1jx0n0l	395	21	1993	1993	CD
cord-104268-q1jx0n0l	395	22	)	)	-RRB-
cord-104268-q1jx0n0l	395	23	has	have	VBZ
cord-104268-q1jx0n0l	395	24	been	be	VBN
cord-104268-q1jx0n0l	395	25	shown	show	VBN
cord-104268-q1jx0n0l	395	26	to	to	TO
cord-104268-q1jx0n0l	395	27	be	be	VB
cord-104268-q1jx0n0l	395	28	properly	properly	RB
cord-104268-q1jx0n0l	395	29	localized	localize	VBN
cord-104268-q1jx0n0l	395	30	in	in	IN
cord-104268-q1jx0n0l	395	31	a	a	DT
cord-104268-q1jx0n0l	395	32	strain	strain	NN
cord-104268-q1jx0n0l	395	33	lacking	lack	VBG
cord-104268-q1jx0n0l	395	34	clathrin	clathrin	NN
cord-104268-q1jx0n0l	395	35	heavy	heavy	JJ
cord-104268-q1jx0n0l	395	36	chains	chain	NNS
cord-104268-q1jx0n0l	395	37	(	(	-LRB-
cord-104268-q1jx0n0l	395	38	Seeger	Seeger	NNP
cord-104268-q1jx0n0l	395	39	and	and	CC
cord-104268-q1jx0n0l	395	40	Payne	Payne	NNP
cord-104268-q1jx0n0l	395	41	,	,	,
cord-104268-q1jx0n0l	395	42	1992	1992	CD
cord-104268-q1jx0n0l	395	43	;	;	:
cord-104268-q1jx0n0l	395	44	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	395	45	and	and	CC
cord-104268-q1jx0n0l	395	46	Payne	Payne	NNP
cord-104268-q1jx0n0l	395	47	,	,	,
cord-104268-q1jx0n0l	395	48	1993	1993	CD
cord-104268-q1jx0n0l	395	49	)	)	-RRB-
cord-104268-q1jx0n0l	395	50	.	.	.
cord-104268-q1jx0n0l	396	1	The	the	DT
cord-104268-q1jx0n0l	396	2	Mnnlp	Mnnlp	NNP
cord-104268-q1jx0n0l	396	3	,	,	,
cord-104268-q1jx0n0l	396	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	396	5	,	,	,
cord-104268-q1jx0n0l	396	6	and	and	CC
cord-104268-q1jx0n0l	396	7	Gdalp	Gdalp	NNP
cord-104268-q1jx0n0l	396	8	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	396	9	appear	appear	VBP
cord-104268-q1jx0n0l	396	10	to	to	TO
cord-104268-q1jx0n0l	396	11	be	be	VB
cord-104268-q1jx0n0l	396	12	in	in	IN
cord-104268-q1jx0n0l	396	13	close	close	JJ
cord-104268-q1jx0n0l	396	14	proximity	proximity	NN
cord-104268-q1jx0n0l	396	15	or	or	CC
cord-104268-q1jx0n0l	396	16	even	even	RB
cord-104268-q1jx0n0l	396	17	in	in	IN
cord-104268-q1jx0n0l	396	18	the	the	DT
cord-104268-q1jx0n0l	396	19	same	same	JJ
cord-104268-q1jx0n0l	396	20	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	396	21	compartment	compartment	NN
cord-104268-q1jx0n0l	396	22	(	(	-LRB-
cord-104268-q1jx0n0l	396	23	this	this	DT
cord-104268-q1jx0n0l	396	24	work	work	NN
cord-104268-q1jx0n0l	396	25	;	;	:
cord-104268-q1jx0n0l	396	26	Abeijon	Abeijon	NNP
cord-104268-q1jx0n0l	396	27	et	et	NNP
cord-104268-q1jx0n0l	396	28	al	al	NNP
cord-104268-q1jx0n0l	396	29	.	.	NNP
cord-104268-q1jx0n0l	396	30	,	,	,
cord-104268-q1jx0n0l	396	31	1989	1989	CD
cord-104268-q1jx0n0l	396	32	;	;	:
cord-104268-q1jx0n0l	397	1	Graham	Graham	NNP
cord-104268-q1jx0n0l	397	2	et	et	NNP
cord-104268-q1jx0n0l	397	3	al	al	NNP
cord-104268-q1jx0n0l	397	4	.	.	NNP
cord-104268-q1jx0n0l	397	5	,	,	,
cord-104268-q1jx0n0l	397	6	1994	1994	CD
cord-104268-q1jx0n0l	397	7	)	)	-RRB-
cord-104268-q1jx0n0l	397	8	.	.	.
cord-104268-q1jx0n0l	398	1	This	this	DT
cord-104268-q1jx0n0l	398	2	raises	raise	VBZ
cord-104268-q1jx0n0l	398	3	the	the	DT
cord-104268-q1jx0n0l	398	4	possibility	possibility	NN
cord-104268-q1jx0n0l	398	5	that	that	IN
cord-104268-q1jx0n0l	398	6	specific	specific	JJ
cord-104268-q1jx0n0l	398	7	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	398	8	membrane	membrane	NN
cord-104268-q1jx0n0l	398	9	proteins	protein	NNS
cord-104268-q1jx0n0l	398	10	showing	show	VBG
cord-104268-q1jx0n0l	398	11	a	a	DT
cord-104268-q1jx0n0l	398	12	similar	similar	JJ
cord-104268-q1jx0n0l	398	13	compartmental	compartmental	JJ
cord-104268-q1jx0n0l	398	14	distribution	distribution	NN
cord-104268-q1jx0n0l	398	15	may	may	MD
cord-104268-q1jx0n0l	398	16	be	be	VB
cord-104268-q1jx0n0l	398	17	retained	retain	VBN
cord-104268-q1jx0n0l	398	18	by	by	IN
cord-104268-q1jx0n0l	398	19	more	more	JJR
cord-104268-q1jx0n0l	398	20	than	than	IN
cord-104268-q1jx0n0l	398	21	one	one	CD
cord-104268-q1jx0n0l	398	22	mechanism	mechanism	NN
cord-104268-q1jx0n0l	398	23	.	.	.
cord-104268-q1jx0n0l	399	1	Our	-PRON-	PRP$
cord-104268-q1jx0n0l	399	2	results	result	NNS
cord-104268-q1jx0n0l	399	3	indicate	indicate	VBP
cord-104268-q1jx0n0l	399	4	that	that	IN
cord-104268-q1jx0n0l	399	5	although	although	IN
cord-104268-q1jx0n0l	399	6	only	only	RB
cord-104268-q1jx0n0l	399	7	the	the	DT
cord-104268-q1jx0n0l	399	8	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	399	9	NH2	NH2	NNP
cord-104268-q1jx0n0l	399	10	terminus	terminus	NN
cord-104268-q1jx0n0l	399	11	has	have	VBZ
cord-104268-q1jx0n0l	399	12	been	be	VBN
cord-104268-q1jx0n0l	399	13	shown	show	VBN
cord-104268-q1jx0n0l	399	14	to	to	TO
cord-104268-q1jx0n0l	399	15	be	be	VB
cord-104268-q1jx0n0l	399	16	required	require	VBN
cord-104268-q1jx0n0l	399	17	for	for	IN
cord-104268-q1jx0n0l	399	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	399	19	retention	retention	NN
cord-104268-q1jx0n0l	399	20	,	,	,
cord-104268-q1jx0n0l	399	21	no	no	DT
cord-104268-q1jx0n0l	399	22	single	single	JJ
cord-104268-q1jx0n0l	399	23	domain	domain	NN
cord-104268-q1jx0n0l	399	24	is	be	VBZ
cord-104268-q1jx0n0l	399	25	able	able	JJ
cord-104268-q1jx0n0l	399	26	to	to	TO
cord-104268-q1jx0n0l	399	27	specify	specify	VB
cord-104268-q1jx0n0l	399	28	correct	correct	JJ
cord-104268-q1jx0n0l	399	29	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	399	30	localization	localization	NN
cord-104268-q1jx0n0l	399	31	.	.	.
cord-104268-q1jx0n0l	400	1	A	a	DT
cord-104268-q1jx0n0l	400	2	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	400	3	protein	protein	NN
cord-104268-q1jx0n0l	400	4	including	include	VBG
cord-104268-q1jx0n0l	400	5	a	a	DT
cord-104268-q1jx0n0l	400	6	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	400	7	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	400	8	tail	tail	NN
cord-104268-q1jx0n0l	400	9	and	and	CC
cord-104268-q1jx0n0l	400	10	enzymatic	enzymatic	JJ
cord-104268-q1jx0n0l	400	11	domain	domain	NN
cord-104268-q1jx0n0l	400	12	(	(	-LRB-
cord-104268-q1jx0n0l	400	13	KD	KD	NNP
cord-104268-q1jx0n0l	400	14	-	-	HYPH
cord-104268-q1jx0n0l	400	15	K	K	NNP
cord-104268-q1jx0n0l	400	16	)	)	-RRB-
cord-104268-q1jx0n0l	400	17	was	be	VBD
cord-104268-q1jx0n0l	400	18	properly	properly	RB
cord-104268-q1jx0n0l	400	19	targeted	target	VBN
cord-104268-q1jx0n0l	400	20	.	.	.
cord-104268-q1jx0n0l	401	1	In	in	IN
cord-104268-q1jx0n0l	401	2	contrast	contrast	NN
cord-104268-q1jx0n0l	401	3	,	,	,
cord-104268-q1jx0n0l	401	4	a	a	DT
cord-104268-q1jx0n0l	401	5	luminal	luminal	JJ
cord-104268-q1jx0n0l	401	6	reporter	reporter	NN
cord-104268-q1jx0n0l	401	7	protein	protein	NN
cord-104268-q1jx0n0l	401	8	could	could	MD
cord-104268-q1jx0n0l	401	9	only	only	RB
cord-104268-q1jx0n0l	401	10	be	be	VB
cord-104268-q1jx0n0l	401	11	properly	properly	RB
cord-104268-q1jx0n0l	401	12	retained	retain	VBN
cord-104268-q1jx0n0l	401	13	in	in	IN
cord-104268-q1jx0n0l	401	14	the	the	DT
cord-104268-q1jx0n0l	401	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	401	16	complex	complex	NN
cord-104268-q1jx0n0l	401	17	by	by	IN
cord-104268-q1jx0n0l	401	18	the	the	DT
cord-104268-q1jx0n0l	401	19	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	401	20	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	401	21	tail	tail	NN
cord-104268-q1jx0n0l	401	22	,	,	,
cord-104268-q1jx0n0l	401	23	plus	plus	CC
cord-104268-q1jx0n0l	401	24	the	the	DT
cord-104268-q1jx0n0l	401	25	membrane	membrane	NN
cord-104268-q1jx0n0l	401	26	-	-	HYPH
cord-104268-q1jx0n0l	401	27	spanning	span	VBG
cord-104268-q1jx0n0l	401	28	and	and	CC
cord-104268-q1jx0n0l	401	29	a	a	DT
cord-104268-q1jx0n0l	401	30	36amino	36amino	CD
cord-104268-q1jx0n0l	401	31	acid	acid	NN
cord-104268-q1jx0n0l	401	32	residue	residue	NN
cord-104268-q1jx0n0l	401	33	stem	stem	NN
cord-104268-q1jx0n0l	401	34	domain	domain	NN
cord-104268-q1jx0n0l	401	35	(	(	-LRB-
cord-104268-q1jx0n0l	401	36	KKKP	KKKP	NNP
cord-104268-q1jx0n0l	401	37	)	)	-RRB-
cord-104268-q1jx0n0l	401	38	.	.	.
cord-104268-q1jx0n0l	402	1	The	the	DT
cord-104268-q1jx0n0l	402	2	entire	entire	JJ
cord-104268-q1jx0n0l	402	3	Kre2p	kre2p	CD
cord-104268-q1jx0n0l	402	4	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	402	5	tail	tail	NN
cord-104268-q1jx0n0l	402	6	and	and	CC
cord-104268-q1jx0n0l	402	7	membrane	membrane	NN
cord-104268-q1jx0n0l	402	8	-	-	HYPH
cord-104268-q1jx0n0l	402	9	spanning	span	VBG
cord-104268-q1jx0n0l	402	10	domain	domain	NN
cord-104268-q1jx0n0l	402	11	were	be	VBD
cord-104268-q1jx0n0l	402	12	not	not	RB
cord-104268-q1jx0n0l	402	13	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	402	14	to	to	TO
cord-104268-q1jx0n0l	402	15	retain	retain	VB
cord-104268-q1jx0n0l	402	16	Pho8p	Pho8p	NNP
cord-104268-q1jx0n0l	402	17	in	in	IN
cord-104268-q1jx0n0l	402	18	the	the	DT
cord-104268-q1jx0n0l	402	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	402	20	complex	complex	NN
cord-104268-q1jx0n0l	402	21	(	(	-LRB-
cord-104268-q1jx0n0l	402	22	KKP	KKP	NNP
cord-104268-q1jx0n0l	402	23	)	)	-RRB-
cord-104268-q1jx0n0l	402	24	.	.	.
cord-104268-q1jx0n0l	403	1	Therefore	therefore	RB
cord-104268-q1jx0n0l	403	2	,	,	,
cord-104268-q1jx0n0l	403	3	it	-PRON-	PRP
cord-104268-q1jx0n0l	403	4	appears	appear	VBZ
cord-104268-q1jx0n0l	403	5	that	that	IN
cord-104268-q1jx0n0l	403	6	a	a	DT
cord-104268-q1jx0n0l	403	7	combination	combination	NN
cord-104268-q1jx0n0l	403	8	of	of	IN
cord-104268-q1jx0n0l	403	9	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	403	10	topological	topological	JJ
cord-104268-q1jx0n0l	403	11	domains	domain	NNS
cord-104268-q1jx0n0l	403	12	is	be	VBZ
cord-104268-q1jx0n0l	403	13	needed	need	VBN
cord-104268-q1jx0n0l	403	14	to	to	TO
cord-104268-q1jx0n0l	403	15	achieve	achieve	VB
cord-104268-q1jx0n0l	403	16	proper	proper	JJ
cord-104268-q1jx0n0l	403	17	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	403	18	localization	localization	NN
cord-104268-q1jx0n0l	403	19	.	.	.
cord-104268-q1jx0n0l	404	1	The	the	DT
cord-104268-q1jx0n0l	404	2	presence	presence	NN
cord-104268-q1jx0n0l	404	3	or	or	CC
cord-104268-q1jx0n0l	404	4	requirement	requirement	NN
cord-104268-q1jx0n0l	404	5	of	of	IN
cord-104268-q1jx0n0l	404	6	more	more	JJR
cord-104268-q1jx0n0l	404	7	than	than	IN
cord-104268-q1jx0n0l	404	8	one	one	CD
cord-104268-q1jx0n0l	404	9	specific	specific	JJ
cord-104268-q1jx0n0l	404	10	targeting	target	VBG
cord-104268-q1jx0n0l	404	11	signal	signal	NN
cord-104268-q1jx0n0l	404	12	in	in	IN
cord-104268-q1jx0n0l	404	13	a	a	DT
cord-104268-q1jx0n0l	404	14	given	give	VBN
cord-104268-q1jx0n0l	404	15	secretory	secretory	JJ
cord-104268-q1jx0n0l	404	16	pathway	pathway	NN
cord-104268-q1jx0n0l	404	17	membraneassociated	membraneassociated	NNP
cord-104268-q1jx0n0l	404	18	protein	protein	NN
cord-104268-q1jx0n0l	404	19	has	have	VBZ
cord-104268-q1jx0n0l	404	20	been	be	VBN
cord-104268-q1jx0n0l	404	21	hinted	hint	VBN
cord-104268-q1jx0n0l	404	22	at	at	IN
cord-104268-q1jx0n0l	404	23	in	in	IN
cord-104268-q1jx0n0l	404	24	a	a	DT
cord-104268-q1jx0n0l	404	25	few	few	JJ
cord-104268-q1jx0n0l	404	26	cases	case	NNS
cord-104268-q1jx0n0l	404	27	recently	recently	RB
cord-104268-q1jx0n0l	404	28	.	.	.
cord-104268-q1jx0n0l	405	1	The	the	DT
cord-104268-q1jx0n0l	405	2	TMD	TMD	NNP
cord-104268-q1jx0n0l	405	3	might	may	MD
cord-104268-q1jx0n0l	405	4	not	not	RB
cord-104268-q1jx0n0l	405	5	be	be	VB
cord-104268-q1jx0n0l	405	6	the	the	DT
cord-104268-q1jx0n0l	405	7	sole	sole	JJ
cord-104268-q1jx0n0l	405	8	targeting	target	VBG
cord-104268-q1jx0n0l	405	9	motif	motif	NN
cord-104268-q1jx0n0l	405	10	present	present	JJ
cord-104268-q1jx0n0l	405	11	in	in	IN
cord-104268-q1jx0n0l	405	12	particular	particular	JJ
cord-104268-q1jx0n0l	405	13	animal	animal	NN
cord-104268-q1jx0n0l	405	14	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	405	15	as	as	IN
cord-104268-q1jx0n0l	405	16	some	some	DT
cord-104268-q1jx0n0l	405	17	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	405	18	and	and	CC
cord-104268-q1jx0n0l	405	19	luminal	luminal	JJ
cord-104268-q1jx0n0l	405	20	sequences	sequence	NNS
cord-104268-q1jx0n0l	405	21	appear	appear	VBP
cord-104268-q1jx0n0l	405	22	to	to	TO
cord-104268-q1jx0n0l	405	23	be	be	VB
cord-104268-q1jx0n0l	405	24	involved	involve	VBN
cord-104268-q1jx0n0l	405	25	in	in	IN
cord-104268-q1jx0n0l	405	26	retention	retention	NN
cord-104268-q1jx0n0l	405	27	(	(	-LRB-
cord-104268-q1jx0n0l	405	28	Munro	Munro	NNP
cord-104268-q1jx0n0l	405	29	,	,	,
cord-104268-q1jx0n0l	405	30	1991	1991	CD
cord-104268-q1jx0n0l	405	31	;	;	:
cord-104268-q1jx0n0l	405	32	Burke	Burke	NNP
cord-104268-q1jx0n0l	405	33	et	et	NNP
cord-104268-q1jx0n0l	405	34	al	al	NNP
cord-104268-q1jx0n0l	405	35	.	.	NNP
cord-104268-q1jx0n0l	405	36	,	,	,
cord-104268-q1jx0n0l	405	37	1992	1992	CD
cord-104268-q1jx0n0l	405	38	;	;	:
cord-104268-q1jx0n0l	405	39	Shaper	shaper	NN
cord-104268-q1jx0n0l	405	40	and	and	CC
cord-104268-q1jx0n0l	405	41	Shaper	Shaper	NNP
cord-104268-q1jx0n0l	405	42	,	,	,
cord-104268-q1jx0n0l	405	43	1992	1992	CD
cord-104268-q1jx0n0l	405	44	;	;	:
cord-104268-q1jx0n0l	405	45	Dahdal	Dahdal	NNP
cord-104268-q1jx0n0l	405	46	et	et	FW
cord-104268-q1jx0n0l	405	47	al	al	NNP
cord-104268-q1jx0n0l	405	48	.	.	NNP
cord-104268-q1jx0n0l	405	49	,	,	,
cord-104268-q1jx0n0l	405	50	1993	1993	CD
cord-104268-q1jx0n0l	405	51	;	;	:
cord-104268-q1jx0n0l	405	52	Burke	Burke	NNP
cord-104268-q1jx0n0l	405	53	et	et	NNP
cord-104268-q1jx0n0l	405	54	al	al	NNP
cord-104268-q1jx0n0l	405	55	.	.	.
cord-104268-q1jx0n0l	406	1	,	,	,
cord-104268-q1jx0n0l	406	2	1994	1994	CD
cord-104268-q1jx0n0l	406	3	;	;	:
cord-104268-q1jx0n0l	406	4	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	406	5	et	et	NNP
cord-104268-q1jx0n0l	406	6	al	al	NNP
cord-104268-q1jx0n0l	406	7	.	.	.
cord-104268-q1jx0n0l	407	1	,	,	,
cord-104268-q1jx0n0l	407	2	1994	1994	CD
cord-104268-q1jx0n0l	407	3	;	;	:
cord-104268-q1jx0n0l	407	4	Low	Low	NNP
cord-104268-q1jx0n0l	407	5	and	and	CC
cord-104268-q1jx0n0l	407	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	407	7	,	,	,
cord-104268-q1jx0n0l	407	8	1994	1994	CD
cord-104268-q1jx0n0l	407	9	)	)	-RRB-
cord-104268-q1jx0n0l	407	10	.	.	.
cord-104268-q1jx0n0l	408	1	The	the	DT
cord-104268-q1jx0n0l	408	2	trans	trans	JJ
cord-104268-q1jx0n0l	408	3	-	-	NNP
cord-104268-q1jx0n0l	408	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	408	5	network	network	NN
cord-104268-q1jx0n0l	408	6	protein	protein	NN
cord-104268-q1jx0n0l	408	7	TGN38	TGN38	NNP
cord-104268-q1jx0n0l	408	8	appears	appear	VBZ
cord-104268-q1jx0n0l	408	9	to	to	TO
cord-104268-q1jx0n0l	408	10	achieve	achieve	VB
cord-104268-q1jx0n0l	408	11	proper	proper	JJ
cord-104268-q1jx0n0l	408	12	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	408	13	localization	localization	NN
cord-104268-q1jx0n0l	408	14	using	use	VBG
cord-104268-q1jx0n0l	408	15	two	two	CD
cord-104268-q1jx0n0l	408	16	nonoverlapping	nonoverlappe	VBG
cord-104268-q1jx0n0l	408	17	targeting	target	VBG
cord-104268-q1jx0n0l	408	18	domains	domain	NNS
cord-104268-q1jx0n0l	408	19	:	:	:
cord-104268-q1jx0n0l	408	20	a	a	DT
cord-104268-q1jx0n0l	408	21	tyrosine	tyrosine	NN
cord-104268-q1jx0n0l	408	22	-	-	HYPH
cord-104268-q1jx0n0l	408	23	based	base	VBN
cord-104268-q1jx0n0l	408	24	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	408	25	retrieval	retrieval	NN
cord-104268-q1jx0n0l	408	26	signal	signal	NN
cord-104268-q1jx0n0l	408	27	and	and	CC
cord-104268-q1jx0n0l	408	28	a	a	DT
cord-104268-q1jx0n0l	408	29	retention	retention	NN
cord-104268-q1jx0n0l	408	30	signal	signal	NN
cord-104268-q1jx0n0l	408	31	found	find	VBN
cord-104268-q1jx0n0l	408	32	in	in	IN
cord-104268-q1jx0n0l	408	33	its	-PRON-	PRP$
cord-104268-q1jx0n0l	408	34	membrane	membrane	NN
cord-104268-q1jx0n0l	408	35	spanning	span	VBG
cord-104268-q1jx0n0l	408	36	domain	domain	NN
cord-104268-q1jx0n0l	408	37	(	(	-LRB-
cord-104268-q1jx0n0l	408	38	Bos	Bos	NNP
cord-104268-q1jx0n0l	408	39	et	et	NNP
cord-104268-q1jx0n0l	408	40	al	al	NNP
cord-104268-q1jx0n0l	408	41	.	.	.
cord-104268-q1jx0n0l	409	1	,	,	,
cord-104268-q1jx0n0l	409	2	1993	1993	CD
cord-104268-q1jx0n0l	409	3	;	;	:
cord-104268-q1jx0n0l	409	4	Humphrey	Humphrey	NNP
cord-104268-q1jx0n0l	409	5	et	et	NNP
cord-104268-q1jx0n0l	409	6	al	al	NNP
cord-104268-q1jx0n0l	409	7	.	.	.
cord-104268-q1jx0n0l	410	1	,	,	,
cord-104268-q1jx0n0l	410	2	1993	1993	CD
cord-104268-q1jx0n0l	410	3	;	;	:
cord-104268-q1jx0n0l	410	4	Wong	Wong	NNP
cord-104268-q1jx0n0l	410	5	and	and	CC
cord-104268-q1jx0n0l	410	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	410	7	,	,	,
cord-104268-q1jx0n0l	410	8	1993	1993	CD
cord-104268-q1jx0n0l	410	9	;	;	:
cord-104268-q1jx0n0l	410	10	Ponnambalam	Ponnambalam	NNP
cord-104268-q1jx0n0l	410	11	et	et	NNP
cord-104268-q1jx0n0l	410	12	al	al	NNP
cord-104268-q1jx0n0l	410	13	.	.	NNP
cord-104268-q1jx0n0l	410	14	,	,	,
cord-104268-q1jx0n0l	410	15	1994	1994	CD
cord-104268-q1jx0n0l	410	16	)	)	-RRB-
cord-104268-q1jx0n0l	410	17	.	.	.
cord-104268-q1jx0n0l	411	1	For	for	IN
cord-104268-q1jx0n0l	411	2	the	the	DT
cord-104268-q1jx0n0l	411	3	CGN	CGN	NNP
cord-104268-q1jx0n0l	411	4	Sed5p	Sed5p	NNP
cord-104268-q1jx0n0l	411	5	syntaxin	syntaxin	NNP
cord-104268-q1jx0n0l	411	6	,	,	,
cord-104268-q1jx0n0l	411	7	the	the	DT
cord-104268-q1jx0n0l	411	8	intracellular	intracellular	JJ
cord-104268-q1jx0n0l	411	9	localization	localization	NN
cord-104268-q1jx0n0l	411	10	is	be	VBZ
cord-104268-q1jx0n0l	411	11	only	only	RB
cord-104268-q1jx0n0l	411	12	partially	partially	RB
cord-104268-q1jx0n0l	411	13	specified	specify	VBN
cord-104268-q1jx0n0l	411	14	by	by	IN
cord-104268-q1jx0n0l	411	15	its	-PRON-	PRP$
cord-104268-q1jx0n0l	411	16	TMD	TMD	NNP
cord-104268-q1jx0n0l	411	17	and	and	CC
cord-104268-q1jx0n0l	411	18	an	an	DT
cord-104268-q1jx0n0l	411	19	additional	additional	JJ
cord-104268-q1jx0n0l	411	20	localization	localization	NN
cord-104268-q1jx0n0l	411	21	signal	signal	NN
cord-104268-q1jx0n0l	411	22	involving	involve	VBG
cord-104268-q1jx0n0l	411	23	its	-PRON-	PRP$
cord-104268-q1jx0n0l	411	24	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	411	25	domain	domain	NN
cord-104268-q1jx0n0l	411	26	appears	appear	VBZ
cord-104268-q1jx0n0l	411	27	to	to	TO
cord-104268-q1jx0n0l	411	28	be	be	VB
cord-104268-q1jx0n0l	411	29	involved	involve	VBN
cord-104268-q1jx0n0l	412	1	(	(	-LRB-
cord-104268-q1jx0n0l	412	2	Banfield	Banfield	NNP
cord-104268-q1jx0n0l	412	3	et	et	NNP
cord-104268-q1jx0n0l	412	4	al	al	NNP
cord-104268-q1jx0n0l	412	5	.	.	NNP
cord-104268-q1jx0n0l	412	6	,	,	,
cord-104268-q1jx0n0l	412	7	1994	1994	CD
cord-104268-q1jx0n0l	412	8	)	)	-RRB-
cord-104268-q1jx0n0l	412	9	.	.	.
cord-104268-q1jx0n0l	413	1	Targeting	target	VBG
cord-104268-q1jx0n0l	413	2	studies	study	NNS
cord-104268-q1jx0n0l	413	3	on	on	IN
cord-104268-q1jx0n0l	413	4	the	the	DT
cord-104268-q1jx0n0l	413	5	p63	p63	CD
cord-104268-q1jx0n0l	413	6	type	type	NN
cord-104268-q1jx0n0l	413	7	II	II	NNP
cord-104268-q1jx0n0l	413	8	membrane	membrane	NN
cord-104268-q1jx0n0l	413	9	protein	protein	NN
cord-104268-q1jx0n0l	413	10	indicate	indicate	VBP
cord-104268-q1jx0n0l	413	11	that	that	IN
cord-104268-q1jx0n0l	413	12	the	the	DT
cord-104268-q1jx0n0l	413	13	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	413	14	,	,	,
cord-104268-q1jx0n0l	413	15	membrane	membrane	NN
cord-104268-q1jx0n0l	413	16	-	-	HYPH
cord-104268-q1jx0n0l	413	17	spanning	span	VBG
cord-104268-q1jx0n0l	413	18	and	and	CC
cord-104268-q1jx0n0l	413	19	luminal	luminal	JJ
cord-104268-q1jx0n0l	413	20	domains	domain	NNS
cord-104268-q1jx0n0l	413	21	are	be	VBP
cord-104268-q1jx0n0l	413	22	all	all	DT
cord-104268-q1jx0n0l	413	23	necessary	necessary	JJ
cord-104268-q1jx0n0l	413	24	for	for	IN
cord-104268-q1jx0n0l	413	25	proper	proper	JJ
cord-104268-q1jx0n0l	413	26	ER	ER	NNP
cord-104268-q1jx0n0l	413	27	-	-	HYPH
cord-104268-q1jx0n0l	413	28	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	413	29	intermediate	intermediate	JJ
cord-104268-q1jx0n0l	413	30	compartment	compartment	NN
cord-104268-q1jx0n0l	413	31	localization	localization	NN
cord-104268-q1jx0n0l	413	32	(	(	-LRB-
cord-104268-q1jx0n0l	413	33	Hauri	Hauri	NNP
cord-104268-q1jx0n0l	413	34	and	and	CC
cord-104268-q1jx0n0l	413	35	Schweizer	Schweizer	NNP
cord-104268-q1jx0n0l	413	36	,	,	,
cord-104268-q1jx0n0l	413	37	1992	1992	CD
cord-104268-q1jx0n0l	413	38	;	;	:
cord-104268-q1jx0n0l	413	39	Schweizer	Schweizer	NNP
cord-104268-q1jx0n0l	413	40	et	et	NNP
cord-104268-q1jx0n0l	413	41	al	al	NNP
cord-104268-q1jx0n0l	413	42	.	.	.
cord-104268-q1jx0n0l	414	1	,	,	,
cord-104268-q1jx0n0l	414	2	1993	1993	CD
cord-104268-q1jx0n0l	415	1	Schweizer	Schweizer	NNP
cord-104268-q1jx0n0l	415	2	et	et	NNP
cord-104268-q1jx0n0l	415	3	al	al	NNP
cord-104268-q1jx0n0l	415	4	.	.	NNP
cord-104268-q1jx0n0l	415	5	,	,	,
cord-104268-q1jx0n0l	415	6	,	,	,
cord-104268-q1jx0n0l	415	7	1994	1994	CD
cord-104268-q1jx0n0l	415	8	.	.	.
cord-104268-q1jx0n0l	416	1	Finally	finally	RB
cord-104268-q1jx0n0l	416	2	,	,	,
cord-104268-q1jx0n0l	416	3	the	the	DT
cord-104268-q1jx0n0l	416	4	Wbpl	Wbpl	NNP
cord-104268-q1jx0n0l	416	5	type	type	NN
cord-104268-q1jx0n0l	416	6	I	-PRON-	PRP
cord-104268-q1jx0n0l	416	7	membrane	membrane	VBP
cord-104268-q1jx0n0l	416	8	protein	protein	NN
cord-104268-q1jx0n0l	416	9	of	of	IN
cord-104268-q1jx0n0l	416	10	the	the	DT
cord-104268-q1jx0n0l	416	11	yeast	yeast	NN
cord-104268-q1jx0n0l	417	1	ER	ER	NNP
cord-104268-q1jx0n0l	417	2	possesses	possess	VBZ
cord-104268-q1jx0n0l	417	3	a	a	DT
cord-104268-q1jx0n0l	417	4	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	417	5	KKXX	KKXX	NNP
cord-104268-q1jx0n0l	417	6	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	417	7	-	-	HYPH
cord-104268-q1jx0n0l	417	8	to	to	IN
cord-104268-q1jx0n0l	417	9	-	-	HYPH
cord-104268-q1jx0n0l	417	10	ER	er	NN
cord-104268-q1jx0n0l	417	11	retrieval	retrieval	NN
cord-104268-q1jx0n0l	417	12	motif	motif	NN
cord-104268-q1jx0n0l	417	13	.	.	.
cord-104268-q1jx0n0l	418	1	When	when	WRB
cord-104268-q1jx0n0l	418	2	this	this	DT
cord-104268-q1jx0n0l	418	3	targeting	targeting	NN
cord-104268-q1jx0n0l	418	4	motif	motif	NN
cord-104268-q1jx0n0l	418	5	is	be	VBZ
cord-104268-q1jx0n0l	418	6	inactivated	inactivate	VBN
cord-104268-q1jx0n0l	418	7	,	,	,
cord-104268-q1jx0n0l	418	8	Wbpl	Wbpl	NNP
cord-104268-q1jx0n0l	418	9	is	be	VBZ
cord-104268-q1jx0n0l	418	10	still	still	RB
cord-104268-q1jx0n0l	418	11	ER	ER	NNP
cord-104268-q1jx0n0l	418	12	retained	retain	VBN
cord-104268-q1jx0n0l	418	13	indicating	indicate	VBG
cord-104268-q1jx0n0l	418	14	that	that	IN
cord-104268-q1jx0n0l	418	15	another	another	DT
cord-104268-q1jx0n0l	418	16	targeting	target	VBG
cord-104268-q1jx0n0l	418	17	signal	signal	NN
cord-104268-q1jx0n0l	418	18	is	be	VBZ
cord-104268-q1jx0n0l	418	19	present	present	JJ
cord-104268-q1jx0n0l	418	20	in	in	IN
cord-104268-q1jx0n0l	418	21	the	the	DT
cord-104268-q1jx0n0l	418	22	protein	protein	NN
cord-104268-q1jx0n0l	418	23	(	(	-LRB-
cord-104268-q1jx0n0l	418	24	Gaynor	Gaynor	NNP
cord-104268-q1jx0n0l	418	25	et	et	NNP
cord-104268-q1jx0n0l	418	26	al	al	NNP
cord-104268-q1jx0n0l	418	27	.	.	NNP
cord-104268-q1jx0n0l	418	28	,	,	,
cord-104268-q1jx0n0l	418	29	1994	1994	CD
cord-104268-q1jx0n0l	418	30	)	)	-RRB-
cord-104268-q1jx0n0l	418	31	.	.	.
cord-104268-q1jx0n0l	419	1	The	the	DT
cord-104268-q1jx0n0l	419	2	fact	fact	NN
cord-104268-q1jx0n0l	419	3	that	that	IN
cord-104268-q1jx0n0l	419	4	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	419	5	requires	require	VBZ
cord-104268-q1jx0n0l	419	6	more	more	JJR
cord-104268-q1jx0n0l	419	7	than	than	IN
cord-104268-q1jx0n0l	419	8	one	one	CD
cord-104268-q1jx0n0l	419	9	topological	topological	JJ
cord-104268-q1jx0n0l	419	10	segment	segment	NN
cord-104268-q1jx0n0l	419	11	to	to	TO
cord-104268-q1jx0n0l	419	12	achieve	achieve	VB
cord-104268-q1jx0n0l	419	13	proper	proper	JJ
cord-104268-q1jx0n0l	419	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	419	15	localization	localization	NN
cord-104268-q1jx0n0l	419	16	,	,	,
cord-104268-q1jx0n0l	419	17	emphasizes	emphasize	VBZ
cord-104268-q1jx0n0l	419	18	that	that	IN
cord-104268-q1jx0n0l	419	19	there	there	EX
cord-104268-q1jx0n0l	419	20	may	may	MD
cord-104268-q1jx0n0l	419	21	be	be	VB
cord-104268-q1jx0n0l	419	22	distinct	distinct	JJ
cord-104268-q1jx0n0l	419	23	,	,	,
cord-104268-q1jx0n0l	419	24	but	but	CC
cord-104268-q1jx0n0l	419	25	not	not	RB
cord-104268-q1jx0n0l	419	26	necessarily	necessarily	RB
cord-104268-q1jx0n0l	419	27	mutually	mutually	RB
cord-104268-q1jx0n0l	419	28	exclusive	exclusive	JJ
cord-104268-q1jx0n0l	419	29	,	,	,
cord-104268-q1jx0n0l	419	30	mechanisms	mechanism	NNS
cord-104268-q1jx0n0l	419	31	of	of	IN
cord-104268-q1jx0n0l	419	32	retention	retention	NN
cord-104268-q1jx0n0l	419	33	.	.	.
cord-104268-q1jx0n0l	420	1	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	420	2	proteins	protein	NNS
cord-104268-q1jx0n0l	420	3	that	that	WDT
cord-104268-q1jx0n0l	420	4	possess	possess	VBP
cord-104268-q1jx0n0l	420	5	a	a	DT
cord-104268-q1jx0n0l	420	6	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	420	7	targeting	targeting	NN
cord-104268-q1jx0n0l	420	8	signal	signal	NN
cord-104268-q1jx0n0l	420	9	have	have	VBP
cord-104268-q1jx0n0l	420	10	been	be	VBN
cord-104268-q1jx0n0l	420	11	proposed	propose	VBN
cord-104268-q1jx0n0l	420	12	to	to	TO
cord-104268-q1jx0n0l	420	13	be	be	VB
cord-104268-q1jx0n0l	420	14	sorted	sort	VBN
cord-104268-q1jx0n0l	420	15	via	via	IN
cord-104268-q1jx0n0l	420	16	a	a	DT
cord-104268-q1jx0n0l	420	17	receptor	receptor	NN
cord-104268-q1jx0n0l	420	18	-	-	HYPH
cord-104268-q1jx0n0l	420	19	mediated	mediate	VBN
cord-104268-q1jx0n0l	420	20	mechanism	mechanism	NN
cord-104268-q1jx0n0l	420	21	(	(	-LRB-
cord-104268-q1jx0n0l	420	22	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	420	23	and	and	CC
cord-104268-q1jx0n0l	420	24	Payne	Payne	NNP
cord-104268-q1jx0n0l	420	25	,	,	,
cord-104268-q1jx0n0l	420	26	1993	1993	CD
cord-104268-q1jx0n0l	420	27	;	;	:
cord-104268-q1jx0n0l	420	28	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	420	29	et	et	NNP
cord-104268-q1jx0n0l	420	30	al	al	NNP
cord-104268-q1jx0n0l	420	31	.	.	.
cord-104268-q1jx0n0l	421	1	,	,	,
cord-104268-q1jx0n0l	421	2	1994	1994	CD
cord-104268-q1jx0n0l	421	3	;	;	:
cord-104268-q1jx0n0l	421	4	Low	Low	NNP
cord-104268-q1jx0n0l	421	5	and	and	CC
cord-104268-q1jx0n0l	421	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	421	7	,	,	,
cord-104268-q1jx0n0l	421	8	1994	1994	CD
cord-104268-q1jx0n0l	421	9	)	)	-RRB-
cord-104268-q1jx0n0l	421	10	.	.	.
cord-104268-q1jx0n0l	422	1	Overexpression	overexpression	NN
cord-104268-q1jx0n0l	422	2	of	of	IN
cord-104268-q1jx0n0l	422	3	the	the	DT
cord-104268-q1jx0n0l	422	4	late	late	JJ
cord-104268-q1jx0n0l	422	5	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	422	6	yeast	yeast	NN
cord-104268-q1jx0n0l	422	7	proteins	protein	NNS
cord-104268-q1jx0n0l	422	8	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	422	9	,	,	,
cord-104268-q1jx0n0l	422	10	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	422	11	,	,	,
cord-104268-q1jx0n0l	422	12	and	and	CC
cord-104268-q1jx0n0l	422	13	DPAP	DPAP	NNP
cord-104268-q1jx0n0l	422	14	A	A	NNP
cord-104268-q1jx0n0l	422	15	leads	lead	VBZ
cord-104268-q1jx0n0l	422	16	to	to	IN
cord-104268-q1jx0n0l	422	17	some	some	DT
cord-104268-q1jx0n0l	422	18	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	422	19	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	422	20	,	,	,
cord-104268-q1jx0n0l	422	21	indicating	indicate	VBG
cord-104268-q1jx0n0l	422	22	saturation	saturation	NN
cord-104268-q1jx0n0l	422	23	of	of	IN
cord-104268-q1jx0n0l	422	24	the	the	DT
cord-104268-q1jx0n0l	422	25	capacity	capacity	NN
cord-104268-q1jx0n0l	422	26	of	of	IN
cord-104268-q1jx0n0l	422	27	a	a	DT
cord-104268-q1jx0n0l	422	28	receptor	receptor	NN
cord-104268-q1jx0n0l	422	29	-	-	HYPH
cord-104268-q1jx0n0l	422	30	based	base	VBN
cord-104268-q1jx0n0l	422	31	sorting	sorting	NN
cord-104268-q1jx0n0l	422	32	process	process	NN
cord-104268-q1jx0n0l	422	33	(	(	-LRB-
cord-104268-q1jx0n0l	422	34	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	422	35	and	and	CC
cord-104268-q1jx0n0l	422	36	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	422	37	,	,	,
cord-104268-q1jx0n0l	422	38	1992	1992	CD
cord-104268-q1jx0n0l	422	39	;	;	:
cord-104268-q1jx0n0l	422	40	Wilcox	Wilcox	NNP
cord-104268-q1jx0n0l	422	41	et	et	NNP
cord-104268-q1jx0n0l	422	42	al	al	NNP
cord-104268-q1jx0n0l	422	43	.	.	NNP
cord-104268-q1jx0n0l	422	44	,	,	,
cord-104268-q1jx0n0l	422	45	1992	1992	CD
cord-104268-q1jx0n0l	422	46	;	;	.
cord-104268-q1jx0n0l	423	1	Nothwehr	Nothwehr	NNP
cord-104268-q1jx0n0l	423	2	et	et	FW
cord-104268-q1jx0n0l	423	3	al	al	NNP
cord-104268-q1jx0n0l	423	4	.	.	.
cord-104268-q1jx0n0l	424	1	,	,	,
cord-104268-q1jx0n0l	424	2	1993	1993	CD
cord-104268-q1jx0n0l	424	3	;	;	:
cord-104268-q1jx0n0l	424	4	Wilsbach	Wilsbach	NNP
cord-104268-q1jx0n0l	424	5	and	and	CC
cord-104268-q1jx0n0l	424	6	Payne	Payne	NNP
cord-104268-q1jx0n0l	424	7	,	,	,
cord-104268-q1jx0n0l	424	8	1993	1993	CD
cord-104268-q1jx0n0l	424	9	)	)	-RRB-
cord-104268-q1jx0n0l	424	10	.	.	.
cord-104268-q1jx0n0l	425	1	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	425	2	overexpression	overexpression	NN
cord-104268-q1jx0n0l	425	3	also	also	RB
cord-104268-q1jx0n0l	425	4	results	result	VBZ
cord-104268-q1jx0n0l	425	5	in	in	IN
cord-104268-q1jx0n0l	425	6	some	some	DT
cord-104268-q1jx0n0l	425	7	mistargeting	mistargeting	NN
cord-104268-q1jx0n0l	425	8	to	to	IN
cord-104268-q1jx0n0l	425	9	the	the	DT
cord-104268-q1jx0n0l	425	10	vacuole	vacuole	NN
cord-104268-q1jx0n0l	425	11	,	,	,
cord-104268-q1jx0n0l	425	12	and	and	CC
cord-104268-q1jx0n0l	425	13	could	could	MD
cord-104268-q1jx0n0l	425	14	be	be	VB
cord-104268-q1jx0n0l	425	15	similarly	similarly	RB
cord-104268-q1jx0n0l	425	16	explained	explain	VBN
cord-104268-q1jx0n0l	425	17	.	.	.
cord-104268-q1jx0n0l	426	1	When	when	WRB
cord-104268-q1jx0n0l	426	2	wild	wild	JJ
cord-104268-q1jx0n0l	426	3	-	-	HYPH
cord-104268-q1jx0n0l	426	4	type	type	NN
cord-104268-q1jx0n0l	426	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	426	6	is	be	VBZ
cord-104268-q1jx0n0l	426	7	expressed	express	VBN
cord-104268-q1jx0n0l	426	8	from	from	IN
cord-104268-q1jx0n0l	426	9	a	a	DT
cord-104268-q1jx0n0l	426	10	21xbased	21xbased	JJ
cord-104268-q1jx0n0l	426	11	multicopy	multicopy	NN
cord-104268-q1jx0n0l	426	12	vector	vector	NN
cord-104268-q1jx0n0l	426	13	in	in	IN
cord-104268-q1jx0n0l	426	14	a	a	DT
cord-104268-q1jx0n0l	426	15	background	background	NN
cord-104268-q1jx0n0l	426	16	with	with	IN
cord-104268-q1jx0n0l	426	17	mutations	mutation	NNS
cord-104268-q1jx0n0l	426	18	in	in	IN
cord-104268-q1jx0n0l	426	19	the	the	DT
cord-104268-q1jx0n0l	426	20	major	major	JJ
cord-104268-q1jx0n0l	426	21	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	426	22	hydrolases	hydrolase	NNS
cord-104268-q1jx0n0l	426	23	,	,	,
cord-104268-q1jx0n0l	426	24	all	all	DT
cord-104268-q1jx0n0l	426	25	positive	positive	JJ
cord-104268-q1jx0n0l	426	26	cells	cell	NNS
cord-104268-q1jx0n0l	426	27	display	display	VBP
cord-104268-q1jx0n0l	426	28	a	a	DT
cord-104268-q1jx0n0l	426	29	punctiform	punctiform	NN
cord-104268-q1jx0n0l	426	30	pattern	pattern	NN
cord-104268-q1jx0n0l	426	31	of	of	IN
cord-104268-q1jx0n0l	426	32	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	426	33	with	with	IN
cord-104268-q1jx0n0l	426	34	15	15	CD
cord-104268-q1jx0n0l	426	35	%	%	NN
cord-104268-q1jx0n0l	426	36	of	of	IN
cord-104268-q1jx0n0l	426	37	the	the	DT
cord-104268-q1jx0n0l	426	38	stained	stained	JJ
cord-104268-q1jx0n0l	426	39	cells	cell	NNS
cord-104268-q1jx0n0l	426	40	also	also	RB
cord-104268-q1jx0n0l	426	41	showing	show	VBG
cord-104268-q1jx0n0l	426	42	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	426	43	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	426	44	.	.	.
cord-104268-q1jx0n0l	427	1	This	this	DT
cord-104268-q1jx0n0l	427	2	small	small	JJ
cord-104268-q1jx0n0l	427	3	percentage	percentage	NN
cord-104268-q1jx0n0l	427	4	of	of	IN
cord-104268-q1jx0n0l	427	5	doubly	doubly	RB
cord-104268-q1jx0n0l	427	6	stained	stain	VBN
cord-104268-q1jx0n0l	427	7	cells	cell	NNS
cord-104268-q1jx0n0l	427	8	are	be	VBP
cord-104268-q1jx0n0l	427	9	likely	likely	RB
cord-104268-q1jx0n0l	427	10	expressing	express	VBG
cord-104268-q1jx0n0l	427	11	high	high	JJ
cord-104268-q1jx0n0l	427	12	levels	level	NNS
cord-104268-q1jx0n0l	427	13	of	of	IN
cord-104268-q1jx0n0l	427	14	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	427	15	as	as	IN
cord-104268-q1jx0n0l	427	16	determined	determine	VBN
cord-104268-q1jx0n0l	427	17	by	by	IN
cord-104268-q1jx0n0l	427	18	the	the	DT
cord-104268-q1jx0n0l	427	19	intensity	intensity	NN
cord-104268-q1jx0n0l	427	20	of	of	IN
cord-104268-q1jx0n0l	427	21	the	the	DT
cord-104268-q1jx0n0l	427	22	fluorescence	fluorescence	NN
cord-104268-q1jx0n0l	427	23	.	.	.
cord-104268-q1jx0n0l	428	1	In	in	IN
cord-104268-q1jx0n0l	428	2	contrast	contrast	NN
cord-104268-q1jx0n0l	428	3	,	,	,
cord-104268-q1jx0n0l	428	4	overexpression	overexpression	NN
cord-104268-q1jx0n0l	428	5	of	of	IN
cord-104268-q1jx0n0l	428	6	animal	animal	NN
cord-104268-q1jx0n0l	428	7	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	428	8	does	do	VBZ
cord-104268-q1jx0n0l	428	9	not	not	RB
cord-104268-q1jx0n0l	428	10	bring	bring	VB
cord-104268-q1jx0n0l	428	11	about	about	RP
cord-104268-q1jx0n0l	428	12	saturation	saturation	NN
cord-104268-q1jx0n0l	428	13	of	of	IN
cord-104268-q1jx0n0l	428	14	the	the	DT
cord-104268-q1jx0n0l	428	15	retention	retention	NN
cord-104268-q1jx0n0l	428	16	mechanism	mechanism	NN
cord-104268-q1jx0n0l	428	17	(	(	-LRB-
cord-104268-q1jx0n0l	428	18	Munro	Munro	NNP
cord-104268-q1jx0n0l	428	19	,	,	,
cord-104268-q1jx0n0l	428	20	1991	1991	CD
cord-104268-q1jx0n0l	428	21	;	;	:
cord-104268-q1jx0n0l	428	22	Aoki	Aoki	NNP
cord-104268-q1jx0n0l	428	23	et	et	NNP
cord-104268-q1jx0n0l	428	24	al	al	NNP
cord-104268-q1jx0n0l	428	25	.	.	NNP
cord-104268-q1jx0n0l	428	26	,	,	,
cord-104268-q1jx0n0l	428	27	1992	1992	CD
cord-104268-q1jx0n0l	428	28	;	;	:
cord-104268-q1jx0n0l	428	29	Burke	Burke	NNP
cord-104268-q1jx0n0l	428	30	et	et	NNP
cord-104268-q1jx0n0l	428	31	al	al	NNP
cord-104268-q1jx0n0l	428	32	.	.	NNP
cord-104268-q1jx0n0l	428	33	,	,	,
cord-104268-q1jx0n0l	428	34	1992	1992	CD
cord-104268-q1jx0n0l	428	35	;	;	:
cord-104268-q1jx0n0l	428	36	Colley	Colley	NNP
cord-104268-q1jx0n0l	428	37	et	et	NNP
cord-104268-q1jx0n0l	428	38	al	al	NNP
cord-104268-q1jx0n0l	428	39	.	.	NNP
cord-104268-q1jx0n0l	428	40	,	,	,
cord-104268-q1jx0n0l	428	41	1992	1992	CD
cord-104268-q1jx0n0l	428	42	;	;	:
cord-104268-q1jx0n0l	428	43	Teasdale	Teasdale	NNP
cord-104268-q1jx0n0l	428	44	et	et	NNP
cord-104268-q1jx0n0l	428	45	al	al	NNP
cord-104268-q1jx0n0l	428	46	.	.	NNP
cord-104268-q1jx0n0l	428	47	,	,	,
cord-104268-q1jx0n0l	428	48	1992	1992	CD
cord-104268-q1jx0n0l	428	49	;	;	:
cord-104268-q1jx0n0l	428	50	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	428	51	et	et	NNP
cord-104268-q1jx0n0l	428	52	al	al	NNP
cord-104268-q1jx0n0l	428	53	.	.	NNP
cord-104268-q1jx0n0l	428	54	,	,	,
cord-104268-q1jx0n0l	428	55	1994	1994	CD
cord-104268-q1jx0n0l	428	56	)	)	-RRB-
cord-104268-q1jx0n0l	428	57	.	.	.
cord-104268-q1jx0n0l	429	1	Interpreting	interpret	VBG
cord-104268-q1jx0n0l	429	2	our	-PRON-	PRP$
cord-104268-q1jx0n0l	429	3	data	datum	NNS
cord-104268-q1jx0n0l	429	4	in	in	IN
cord-104268-q1jx0n0l	429	5	the	the	DT
cord-104268-q1jx0n0l	429	6	simplest	simple	JJS
cord-104268-q1jx0n0l	429	7	way	way	NN
cord-104268-q1jx0n0l	429	8	,	,	,
cord-104268-q1jx0n0l	429	9	the	the	DT
cord-104268-q1jx0n0l	429	10	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	429	11	segment	segment	NN
cord-104268-q1jx0n0l	429	12	encompassing	encompass	VBG
cord-104268-q1jx0n0l	429	13	the	the	DT
cord-104268-q1jx0n0l	429	14	NH	NH	NNP
cord-104268-q1jx0n0l	429	15	2	2	CD
cord-104268-q1jx0n0l	429	16	terminus	terminus	NN
cord-104268-q1jx0n0l	429	17	along	along	IN
cord-104268-q1jx0n0l	429	18	with	with	IN
cord-104268-q1jx0n0l	429	19	the	the	DT
cord-104268-q1jx0n0l	429	20	TMD	TMD	NNP
cord-104268-q1jx0n0l	429	21	and	and	CC
cord-104268-q1jx0n0l	429	22	stem	stem	NN
cord-104268-q1jx0n0l	429	23	region	region	NN
cord-104268-q1jx0n0l	429	24	could	could	MD
cord-104268-q1jx0n0l	429	25	interact	interact	VB
cord-104268-q1jx0n0l	429	26	directly	directly	RB
cord-104268-q1jx0n0l	429	27	with	with	IN
cord-104268-q1jx0n0l	429	28	a	a	DT
cord-104268-q1jx0n0l	429	29	medial	medial	JJ
cord-104268-q1jx0n0l	429	30	-	-	HYPH
cord-104268-q1jx0n0l	429	31	Golgi	golgi	NN
cord-104268-q1jx0n0l	429	32	localized	localized	JJ
cord-104268-q1jx0n0l	429	33	receptor	receptor	NN
cord-104268-q1jx0n0l	429	34	spanning	span	VBG
cord-104268-q1jx0n0l	429	35	both	both	DT
cord-104268-q1jx0n0l	429	36	sides	side	NNS
cord-104268-q1jx0n0l	429	37	of	of	IN
cord-104268-q1jx0n0l	429	38	the	the	DT
cord-104268-q1jx0n0l	429	39	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	429	40	lipid	lipid	NN
cord-104268-q1jx0n0l	429	41	bilayer	bilayer	NN
cord-104268-q1jx0n0l	429	42	.	.	.
cord-104268-q1jx0n0l	430	1	For	for	IN
cord-104268-q1jx0n0l	430	2	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	430	3	to	to	TO
cord-104268-q1jx0n0l	430	4	be	be	VB
cord-104268-q1jx0n0l	430	5	retained	retain	VBN
cord-104268-q1jx0n0l	430	6	in	in	IN
cord-104268-q1jx0n0l	430	7	the	the	DT
cord-104268-q1jx0n0l	430	8	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	430	9	,	,	,
cord-104268-q1jx0n0l	430	10	an	an	DT
cord-104268-q1jx0n0l	430	11	interaction	interaction	NN
cord-104268-q1jx0n0l	430	12	between	between	IN
cord-104268-q1jx0n0l	430	13	the	the	DT
cord-104268-q1jx0n0l	430	14	putative	putative	JJ
cord-104268-q1jx0n0l	430	15	receptor	receptor	NN
cord-104268-q1jx0n0l	430	16	and	and	CC
cord-104268-q1jx0n0l	430	17	the	the	DT
cord-104268-q1jx0n0l	430	18	NH2-terminal	nh2-terminal	JJ
cord-104268-q1jx0n0l	430	19	domain	domain	NN
cord-104268-q1jx0n0l	430	20	is	be	VBZ
cord-104268-q1jx0n0l	430	21	essential	essential	JJ
cord-104268-q1jx0n0l	430	22	.	.	.
cord-104268-q1jx0n0l	431	1	Part	part	NN
cord-104268-q1jx0n0l	431	2	of	of	IN
cord-104268-q1jx0n0l	431	3	the	the	DT
cord-104268-q1jx0n0l	431	4	presumed	presume	VBN
cord-104268-q1jx0n0l	431	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	431	6	receptor	receptor	NN
cord-104268-q1jx0n0l	431	7	complex	complex	NN
cord-104268-q1jx0n0l	431	8	could	could	MD
cord-104268-q1jx0n0l	431	9	be	be	VB
cord-104268-q1jx0n0l	431	10	part	part	NN
cord-104268-q1jx0n0l	431	11	of	of	IN
cord-104268-q1jx0n0l	431	12	the	the	DT
cord-104268-q1jx0n0l	431	13	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	431	14	extracisternal	extracisternal	JJ
cord-104268-q1jx0n0l	431	15	space	space	NN
cord-104268-q1jx0n0l	431	16	matrix	matrix	NN
cord-104268-q1jx0n0l	431	17	which	which	WDT
cord-104268-q1jx0n0l	431	18	in	in	IN
cord-104268-q1jx0n0l	431	19	mammals	mammal	NNS
cord-104268-q1jx0n0l	431	20	was	be	VBD
cord-104268-q1jx0n0l	431	21	recently	recently	RB
cord-104268-q1jx0n0l	431	22	shown	show	VBN
cord-104268-q1jx0n0l	431	23	to	to	TO
cord-104268-q1jx0n0l	431	24	bind	bind	VB
cord-104268-q1jx0n0l	431	25	medial	medial	JJ
cord-104268-q1jx0n0l	431	26	-	-	HYPH
cord-104268-q1jx0n0l	431	27	Golgi	golgi	NN
cord-104268-q1jx0n0l	431	28	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	431	29	presumably	presumably	RB
cord-104268-q1jx0n0l	431	30	through	through	IN
cord-104268-q1jx0n0l	431	31	their	-PRON-	PRP$
cord-104268-q1jx0n0l	431	32	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	431	33	tails	tail	NNS
cord-104268-q1jx0n0l	431	34	.	.	.
cord-104268-q1jx0n0l	432	1	Interestingly	interestingly	RB
cord-104268-q1jx0n0l	432	2	,	,	,
cord-104268-q1jx0n0l	432	3	examination	examination	NN
cord-104268-q1jx0n0l	432	4	of	of	IN
cord-104268-q1jx0n0l	432	5	the	the	DT
cord-104268-q1jx0n0l	432	6	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	432	7	NHz	nhz	NN
cord-104268-q1jx0n0l	432	8	-	-	HYPH
cord-104268-q1jx0n0l	432	9	terminal	terminal	NN
cord-104268-q1jx0n0l	432	10	domains	domain	NNS
cord-104268-q1jx0n0l	432	11	of	of	IN
cord-104268-q1jx0n0l	432	12	the	the	DT
cord-104268-q1jx0n0l	432	13	six	six	CD
cord-104268-q1jx0n0l	432	14	members	member	NNS
cord-104268-q1jx0n0l	432	15	of	of	IN
cord-104268-q1jx0n0l	432	16	the	the	DT
cord-104268-q1jx0n0l	432	17	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	432	18	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	432	19	family	family	NN
cord-104268-q1jx0n0l	432	20	(	(	-LRB-
cord-104268-q1jx0n0l	432	21	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	432	22	et	et	NNP
cord-104268-q1jx0n0l	432	23	al	al	NNP
cord-104268-q1jx0n0l	432	24	.	.	NNP
cord-104268-q1jx0n0l	432	25	,	,	,
cord-104268-q1jx0n0l	432	26	1993	1993	CD
cord-104268-q1jx0n0l	432	27	;	;	:
cord-104268-q1jx0n0l	432	28	Mallet	Mallet	NNP
cord-104268-q1jx0n0l	432	29	et	et	NNP
cord-104268-q1jx0n0l	432	30	al	al	NNP
cord-104268-q1jx0n0l	432	31	.	.	NNP
cord-104268-q1jx0n0l	432	32	,	,	,
cord-104268-q1jx0n0l	432	33	1994	1994	CD
cord-104268-q1jx0n0l	432	34	)	)	-RRB-
cord-104268-q1jx0n0l	432	35	reveals	reveal	VBZ
cord-104268-q1jx0n0l	432	36	that	that	IN
cord-104268-q1jx0n0l	432	37	the	the	DT
cord-104268-q1jx0n0l	432	38	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	432	39	(	(	-LRB-
cord-104268-q1jx0n0l	432	40	MALFLSKRLLR	MALFLSKRLLR	NNP
cord-104268-q1jx0n0l	432	41	)	)	-RRB-
cord-104268-q1jx0n0l	432	42	sequence	sequence	NN
cord-104268-q1jx0n0l	432	43	resembles	resemble	VBZ
cord-104268-q1jx0n0l	432	44	only	only	RB
cord-104268-q1jx0n0l	432	45	that	that	DT
cord-104268-q1jx0n0l	432	46	of	of	IN
cord-104268-q1jx0n0l	432	47	Ktr4p	Ktr4p	NNS
cord-104268-q1jx0n0l	432	48	(	(	-LRB-
cord-104268-q1jx0n0l	432	49	MRFLSKRILK	MRFLSKRILK	NNP
cord-104268-q1jx0n0l	432	50	;	;	:
cord-104268-q1jx0n0l	432	51	Mallet	Mallet	NNP
cord-104268-q1jx0n0l	432	52	et	et	NNP
cord-104268-q1jx0n0l	432	53	al	al	NNP
cord-104268-q1jx0n0l	432	54	.	.	NNP
cord-104268-q1jx0n0l	432	55	,	,	,
cord-104268-q1jx0n0l	432	56	1994	1994	CD
cord-104268-q1jx0n0l	432	57	)	)	-RRB-
cord-104268-q1jx0n0l	432	58	where	where	WRB
cord-104268-q1jx0n0l	432	59	the	the	DT
cord-104268-q1jx0n0l	432	60	sequence	sequence	NN
cord-104268-q1jx0n0l	432	61	FLSKR(I	FLSKR(I	NNP
cord-104268-q1jx0n0l	432	62	/	/	SYM
cord-104268-q1jx0n0l	432	63	L)L(K	L)L(K	NNP
cord-104268-q1jx0n0l	432	64	/	/	SYM
cord-104268-q1jx0n0l	432	65	R	R	NNP
cord-104268-q1jx0n0l	432	66	)	)	-RRB-
cord-104268-q1jx0n0l	432	67	is	be	VBZ
cord-104268-q1jx0n0l	432	68	conserved	conserve	VBN
cord-104268-q1jx0n0l	432	69	in	in	IN
cord-104268-q1jx0n0l	432	70	both	both	DT
cord-104268-q1jx0n0l	432	71	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	432	72	.	.	.
cord-104268-q1jx0n0l	433	1	This	this	DT
cord-104268-q1jx0n0l	433	2	may	may	MD
cord-104268-q1jx0n0l	433	3	imply	imply	VB
cord-104268-q1jx0n0l	433	4	a	a	DT
cord-104268-q1jx0n0l	433	5	common	common	JJ
cord-104268-q1jx0n0l	433	6	recep	recep	NNP
cord-104268-q1jx0n0l	433	7	-	-	HYPH
cord-104268-q1jx0n0l	433	8	tor	tor	NN
cord-104268-q1jx0n0l	433	9	for	for	IN
cord-104268-q1jx0n0l	433	10	the	the	DT
cord-104268-q1jx0n0l	433	11	two	two	CD
cord-104268-q1jx0n0l	433	12	proteins	protein	NNS
cord-104268-q1jx0n0l	433	13	.	.	.
cord-104268-q1jx0n0l	434	1	Interaction	interaction	NN
cord-104268-q1jx0n0l	434	2	of	of	IN
cord-104268-q1jx0n0l	434	3	the	the	DT
cord-104268-q1jx0n0l	434	4	presumed	presume	VBN
cord-104268-q1jx0n0l	434	5	receptor	receptor	NN
cord-104268-q1jx0n0l	434	6	protein	protein	NN
cord-104268-q1jx0n0l	434	7	with	with	IN
cord-104268-q1jx0n0l	434	8	a	a	DT
cord-104268-q1jx0n0l	434	9	chimeric	chimeric	JJ
cord-104268-q1jx0n0l	434	10	construct	construct	NN
cord-104268-q1jx0n0l	434	11	lacking	lack	VBG
cord-104268-q1jx0n0l	434	12	the	the	DT
cord-104268-q1jx0n0l	434	13	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	434	14	stem	stem	NN
cord-104268-q1jx0n0l	434	15	domain	domain	NN
cord-104268-q1jx0n0l	434	16	but	but	CC
cord-104268-q1jx0n0l	434	17	including	include	VBG
cord-104268-q1jx0n0l	434	18	a	a	DT
cord-104268-q1jx0n0l	434	19	reporter	reporter	NN
cord-104268-q1jx0n0l	434	20	luminal	luminal	JJ
cord-104268-q1jx0n0l	434	21	domain	domain	NN
cord-104268-q1jx0n0l	434	22	(	(	-LRB-
cord-104268-q1jx0n0l	434	23	KKP	KKP	NNP
cord-104268-q1jx0n0l	434	24	)	)	-RRB-
cord-104268-q1jx0n0l	434	25	may	may	MD
cord-104268-q1jx0n0l	434	26	be	be	VB
cord-104268-q1jx0n0l	434	27	partial	partial	JJ
cord-104268-q1jx0n0l	434	28	,	,	,
cord-104268-q1jx0n0l	434	29	and	and	CC
cord-104268-q1jx0n0l	434	30	insufficient	insufficient	JJ
cord-104268-q1jx0n0l	434	31	to	to	TO
cord-104268-q1jx0n0l	434	32	retain	retain	VB
cord-104268-q1jx0n0l	434	33	the	the	DT
cord-104268-q1jx0n0l	434	34	fusion	fusion	NN
cord-104268-q1jx0n0l	434	35	protein	protein	NN
cord-104268-q1jx0n0l	434	36	in	in	IN
cord-104268-q1jx0n0l	434	37	the	the	DT
cord-104268-q1jx0n0l	434	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	434	39	complex	complex	NN
cord-104268-q1jx0n0l	434	40	.	.	.
cord-104268-q1jx0n0l	435	1	To	to	TO
cord-104268-q1jx0n0l	435	2	achieve	achieve	VB
cord-104268-q1jx0n0l	435	3	full	full	JJ
cord-104268-q1jx0n0l	435	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	435	5	retention	retention	NN
cord-104268-q1jx0n0l	435	6	of	of	IN
cord-104268-q1jx0n0l	435	7	a	a	DT
cord-104268-q1jx0n0l	435	8	reporter	reporter	NN
cord-104268-q1jx0n0l	435	9	luminal	luminal	JJ
cord-104268-q1jx0n0l	435	10	protein	protein	NN
cord-104268-q1jx0n0l	435	11	,	,	,
cord-104268-q1jx0n0l	435	12	interaction	interaction	NN
cord-104268-q1jx0n0l	435	13	of	of	IN
cord-104268-q1jx0n0l	435	14	the	the	DT
cord-104268-q1jx0n0l	435	15	postulated	postulate	VBN
cord-104268-q1jx0n0l	435	16	receptor	receptor	NN
cord-104268-q1jx0n0l	435	17	with	with	IN
cord-104268-q1jx0n0l	435	18	the	the	DT
cord-104268-q1jx0n0l	435	19	three	three	CD
cord-104268-q1jx0n0l	435	20	nonenzymatic	nonenzymatic	JJ
cord-104268-q1jx0n0l	435	21	domains	domain	NNS
cord-104268-q1jx0n0l	435	22	would	would	MD
cord-104268-q1jx0n0l	435	23	be	be	VB
cord-104268-q1jx0n0l	435	24	required	require	VBN
cord-104268-q1jx0n0l	435	25	.	.	.
cord-104268-q1jx0n0l	436	1	In	in	IN
cord-104268-q1jx0n0l	436	2	the	the	DT
cord-104268-q1jx0n0l	436	3	case	case	NN
cord-104268-q1jx0n0l	436	4	of	of	IN
cord-104268-q1jx0n0l	436	5	KD	KD	NNP
cord-104268-q1jx0n0l	436	6	-	-	HYPH
cord-104268-q1jx0n0l	436	7	K	K	NNP
cord-104268-q1jx0n0l	436	8	,	,	,
cord-104268-q1jx0n0l	436	9	only	only	JJ
cord-104268-q1jx0n0l	436	10	partial	partial	JJ
cord-104268-q1jx0n0l	436	11	interaction	interaction	NN
cord-104268-q1jx0n0l	436	12	would	would	MD
cord-104268-q1jx0n0l	436	13	occur	occur	VB
cord-104268-q1jx0n0l	436	14	with	with	IN
cord-104268-q1jx0n0l	436	15	the	the	DT
cord-104268-q1jx0n0l	436	16	putative	putative	JJ
cord-104268-q1jx0n0l	436	17	receptor	receptor	NN
cord-104268-q1jx0n0l	436	18	and	and	CC
cord-104268-q1jx0n0l	436	19	the	the	DT
cord-104268-q1jx0n0l	436	20	observed	observed	JJ
cord-104268-q1jx0n0l	436	21	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	436	22	retention	retention	NN
cord-104268-q1jx0n0l	436	23	may	may	MD
cord-104268-q1jx0n0l	436	24	now	now	RB
cord-104268-q1jx0n0l	436	25	reveal	reveal	VB
cord-104268-q1jx0n0l	436	26	an	an	DT
cord-104268-q1jx0n0l	436	27	additional	additional	JJ
cord-104268-q1jx0n0l	436	28	mechanism	mechanism	NN
cord-104268-q1jx0n0l	436	29	involving	involve	VBG
cord-104268-q1jx0n0l	436	30	a	a	DT
cord-104268-q1jx0n0l	436	31	segment	segment	NN
cord-104268-q1jx0n0l	436	32	of	of	IN
cord-104268-q1jx0n0l	436	33	the	the	DT
cord-104268-q1jx0n0l	436	34	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	436	35	luminal	luminal	JJ
cord-104268-q1jx0n0l	436	36	region	region	NN
cord-104268-q1jx0n0l	436	37	possibly	possibly	RB
cord-104268-q1jx0n0l	436	38	by	by	IN
cord-104268-q1jx0n0l	436	39	oligomerization	oligomerization	NN
cord-104268-q1jx0n0l	436	40	/	/	SYM
cord-104268-q1jx0n0l	436	41	kin	kin	NN
cord-104268-q1jx0n0l	436	42	recognition	recognition	NN
cord-104268-q1jx0n0l	436	43	that	that	WDT
cord-104268-q1jx0n0l	436	44	has	have	VBZ
cord-104268-q1jx0n0l	436	45	been	be	VBN
cord-104268-q1jx0n0l	436	46	implicated	implicate	VBN
cord-104268-q1jx0n0l	436	47	in	in	IN
cord-104268-q1jx0n0l	436	48	the	the	DT
cord-104268-q1jx0n0l	436	49	retention	retention	NN
cord-104268-q1jx0n0l	436	50	of	of	IN
cord-104268-q1jx0n0l	436	51	certain	certain	JJ
cord-104268-q1jx0n0l	436	52	mammalian	mammalian	JJ
cord-104268-q1jx0n0l	436	53	Golgi'membrane	Golgi'membrane	NNP
cord-104268-q1jx0n0l	436	54	proteins	protein	NNS
cord-104268-q1jx0n0l	436	55	.	.	.
cord-104268-q1jx0n0l	437	1	It	-PRON-	PRP
cord-104268-q1jx0n0l	437	2	has	have	VBZ
cord-104268-q1jx0n0l	437	3	been	be	VBN
cord-104268-q1jx0n0l	437	4	proposed	propose	VBN
cord-104268-q1jx0n0l	437	5	that	that	IN
cord-104268-q1jx0n0l	437	6	protein	protein	NN
cord-104268-q1jx0n0l	437	7	oligomers	oligomer	NNS
cord-104268-q1jx0n0l	437	8	are	be	VBP
cord-104268-q1jx0n0l	437	9	assembled	assemble	VBN
cord-104268-q1jx0n0l	437	10	through	through	IN
cord-104268-q1jx0n0l	437	11	their	-PRON-	PRP$
cord-104268-q1jx0n0l	437	12	TMD	TMD	NNP
cord-104268-q1jx0n0l	437	13	and/or	and/or	CC
cord-104268-q1jx0n0l	437	14	luminal	luminal	JJ
cord-104268-q1jx0n0l	437	15	domains	domain	NNS
cord-104268-q1jx0n0l	437	16	in	in	IN
cord-104268-q1jx0n0l	437	17	a	a	DT
cord-104268-q1jx0n0l	437	18	particular	particular	JJ
cord-104268-q1jx0n0l	437	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	437	20	cisternae	cisternae	NNP
cord-104268-q1jx0n0l	437	21	and	and	CC
cord-104268-q1jx0n0l	437	22	because	because	IN
cord-104268-q1jx0n0l	437	23	of	of	IN
cord-104268-q1jx0n0l	437	24	their	-PRON-	PRP$
cord-104268-q1jx0n0l	437	25	highorder	highorder	NN
cord-104268-q1jx0n0l	437	26	structure	structure	NN
cord-104268-q1jx0n0l	437	27	are	be	VBP
cord-104268-q1jx0n0l	437	28	consequently	consequently	RB
cord-104268-q1jx0n0l	437	29	excluded	exclude	VBN
cord-104268-q1jx0n0l	437	30	from	from	IN
cord-104268-q1jx0n0l	437	31	entering	enter	VBG
cord-104268-q1jx0n0l	437	32	forward	forward	RB
cord-104268-q1jx0n0l	437	33	-	-	HYPH
cord-104268-q1jx0n0l	437	34	moving	move	VBG
cord-104268-q1jx0n0l	437	35	secretory	secretory	JJ
cord-104268-q1jx0n0l	437	36	vesicles	vesicle	NNS
cord-104268-q1jx0n0l	437	37	(	(	-LRB-
cord-104268-q1jx0n0l	437	38	Weisz	Weisz	NNP
cord-104268-q1jx0n0l	437	39	et	et	NNP
cord-104268-q1jx0n0l	437	40	al	al	NNP
cord-104268-q1jx0n0l	437	41	.	.	NNP
cord-104268-q1jx0n0l	437	42	,	,	,
cord-104268-q1jx0n0l	437	43	1993	1993	CD
cord-104268-q1jx0n0l	437	44	;	;	:
cord-104268-q1jx0n0l	437	45	Gleeson	Gleeson	NNP
cord-104268-q1jx0n0l	437	46	et	et	NNP
cord-104268-q1jx0n0l	437	47	al	al	NNP
cord-104268-q1jx0n0l	437	48	.	.	.
cord-104268-q1jx0n0l	438	1	,	,	,
cord-104268-q1jx0n0l	438	2	1994	1994	CD
cord-104268-q1jx0n0l	438	3	;	;	:
cord-104268-q1jx0n0l	438	4	Low	Low	NNP
cord-104268-q1jx0n0l	438	5	and	and	CC
cord-104268-q1jx0n0l	438	6	Hong	Hong	NNP
cord-104268-q1jx0n0l	438	7	,	,	,
cord-104268-q1jx0n0l	438	8	1994	1994	CD
cord-104268-q1jx0n0l	438	9	;	;	:
cord-104268-q1jx0n0l	438	10	Nilsson	Nilsson	NNP
cord-104268-q1jx0n0l	438	11	et	et	NNP
cord-104268-q1jx0n0l	438	12	al	al	NNP
cord-104268-q1jx0n0l	438	13	.	.	NNP
cord-104268-q1jx0n0l	438	14	,	,	,
cord-104268-q1jx0n0l	438	15	1994	1994	CD
cord-104268-q1jx0n0l	438	16	;	;	:
cord-104268-q1jx0n0l	438	17	Schweizer	Schweizer	NNP
cord-104268-q1jx0n0l	438	18	et	et	NNP
cord-104268-q1jx0n0l	438	19	al	al	NNP
cord-104268-q1jx0n0l	438	20	.	.	NNP
cord-104268-q1jx0n0l	438	21	,	,	,
cord-104268-q1jx0n0l	438	22	1994	1994	CD
cord-104268-q1jx0n0l	438	23	;	;	:
cord-104268-q1jx0n0l	438	24	Yamaguchi	Yamaguchi	NNP
cord-104268-q1jx0n0l	438	25	and	and	CC
cord-104268-q1jx0n0l	438	26	Fukuda	Fukuda	NNP
cord-104268-q1jx0n0l	438	27	,	,	,
cord-104268-q1jx0n0l	438	28	1995	1995	CD
cord-104268-q1jx0n0l	438	29	)	)	-RRB-
cord-104268-q1jx0n0l	438	30	.	.	.
cord-104268-q1jx0n0l	439	1	Applying	apply	VBG
cord-104268-q1jx0n0l	439	2	such	such	PDT
cord-104268-q1jx0n0l	439	3	a	a	DT
cord-104268-q1jx0n0l	439	4	model	model	NN
cord-104268-q1jx0n0l	439	5	to	to	IN
cord-104268-q1jx0n0l	439	6	a	a	DT
cord-104268-q1jx0n0l	439	7	functionally	functionally	RB
cord-104268-q1jx0n0l	439	8	active	active	JJ
cord-104268-q1jx0n0l	439	9	Kre2	Kre2	NNP
cord-104268-q1jx0n0l	439	10	protein	protein	NN
cord-104268-q1jx0n0l	439	11	involves	involve	VBZ
cord-104268-q1jx0n0l	439	12	self	self	NN
cord-104268-q1jx0n0l	439	13	-	-	HYPH
cord-104268-q1jx0n0l	439	14	association	association	NN
cord-104268-q1jx0n0l	439	15	or	or	CC
cord-104268-q1jx0n0l	439	16	formation	formation	NN
cord-104268-q1jx0n0l	439	17	of	of	IN
cord-104268-q1jx0n0l	439	18	heterooligomers	heterooligomer	NNS
cord-104268-q1jx0n0l	439	19	between	between	IN
cord-104268-q1jx0n0l	439	20	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	439	21	and	and	CC
cord-104268-q1jx0n0l	439	22	other	other	JJ
cord-104268-q1jx0n0l	439	23	medial	medial	JJ
cord-104268-q1jx0n0l	439	24	-	-	HYPH
cord-104268-q1jx0n0l	439	25	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	439	26	protein(s	protein(s	NNP
cord-104268-q1jx0n0l	439	27	)	)	-RRB-
cord-104268-q1jx0n0l	440	1	either	either	CC
cord-104268-q1jx0n0l	440	2	through	through	IN
cord-104268-q1jx0n0l	440	3	the	the	DT
cord-104268-q1jx0n0l	440	4	catalytic	catalytic	JJ
cord-104268-q1jx0n0l	440	5	domain	domain	NN
cord-104268-q1jx0n0l	440	6	or	or	CC
cord-104268-q1jx0n0l	440	7	possibly	possibly	RB
cord-104268-q1jx0n0l	440	8	with	with	IN
cord-104268-q1jx0n0l	440	9	some	some	DT
cord-104268-q1jx0n0l	440	10	contiguous	contiguous	JJ
cord-104268-q1jx0n0l	440	11	stem	stem	NN
cord-104268-q1jx0n0l	440	12	sequences	sequence	NNS
cord-104268-q1jx0n0l	440	13	retained	retain	VBN
cord-104268-q1jx0n0l	440	14	in	in	IN
cord-104268-q1jx0n0l	440	15	construct	construct	NN
cord-104268-q1jx0n0l	440	16	Overall	Overall	NNP
cord-104268-q1jx0n0l	440	17	,	,	,
cord-104268-q1jx0n0l	440	18	our	-PRON-	PRP$
cord-104268-q1jx0n0l	440	19	results	result	NNS
cord-104268-q1jx0n0l	440	20	suggest	suggest	VBP
cord-104268-q1jx0n0l	440	21	that	that	IN
cord-104268-q1jx0n0l	440	22	proper	proper	JJ
cord-104268-q1jx0n0l	440	23	targeting	targeting	NN
cord-104268-q1jx0n0l	440	24	of	of	IN
cord-104268-q1jx0n0l	440	25	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	440	26	in	in	IN
cord-104268-q1jx0n0l	440	27	the	the	DT
cord-104268-q1jx0n0l	440	28	yeast	yeast	NN
cord-104268-q1jx0n0l	440	29	medial	medial	JJ
cord-104268-q1jx0n0l	440	30	-	-	HYPH
cord-104268-q1jx0n0l	440	31	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	440	32	may	may	MD
cord-104268-q1jx0n0l	440	33	involve	involve	VB
cord-104268-q1jx0n0l	440	34	two	two	CD
cord-104268-q1jx0n0l	440	35	different	different	JJ
cord-104268-q1jx0n0l	440	36	mechanisms	mechanism	NNS
cord-104268-q1jx0n0l	440	37	.	.	.
cord-104268-q1jx0n0l	441	1	The	the	DT
cord-104268-q1jx0n0l	441	2	saturability	saturability	NN
cord-104268-q1jx0n0l	441	3	of	of	IN
cord-104268-q1jx0n0l	441	4	the	the	DT
cord-104268-q1jx0n0l	441	5	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	441	6	retention	retention	NN
cord-104268-q1jx0n0l	441	7	system	system	NN
cord-104268-q1jx0n0l	441	8	implies	imply	VBZ
cord-104268-q1jx0n0l	441	9	a	a	DT
cord-104268-q1jx0n0l	441	10	receptor	receptor	NN
cord-104268-q1jx0n0l	441	11	-	-	HYPH
cord-104268-q1jx0n0l	441	12	based	base	VBN
cord-104268-q1jx0n0l	441	13	retention	retention	NN
cord-104268-q1jx0n0l	441	14	mechanism	mechanism	NN
cord-104268-q1jx0n0l	441	15	that	that	WDT
cord-104268-q1jx0n0l	441	16	could	could	MD
cord-104268-q1jx0n0l	441	17	involve	involve	VB
cord-104268-q1jx0n0l	441	18	protein	protein	NN
cord-104268-q1jx0n0l	441	19	-	-	HYPH
cord-104268-q1jx0n0l	441	20	protein	protein	NN
cord-104268-q1jx0n0l	441	21	interactions	interaction	NNS
cord-104268-q1jx0n0l	441	22	over	over	IN
cord-104268-q1jx0n0l	441	23	a	a	DT
cord-104268-q1jx0n0l	441	24	tail	tail	NN
cord-104268-q1jx0n0l	441	25	/	/	SYM
cord-104268-q1jx0n0l	441	26	TMD/	tmd/	JJ
cord-104268-q1jx0n0l	441	27	partial	partial	JJ
cord-104268-q1jx0n0l	441	28	stem	stem	NN
cord-104268-q1jx0n0l	441	29	region	region	NN
cord-104268-q1jx0n0l	441	30	.	.	.
cord-104268-q1jx0n0l	442	1	In	in	IN
cord-104268-q1jx0n0l	442	2	addition	addition	NN
cord-104268-q1jx0n0l	442	3	,	,	,
cord-104268-q1jx0n0l	442	4	a	a	DT
cord-104268-q1jx0n0l	442	5	second	second	JJ
cord-104268-q1jx0n0l	442	6	mechanism	mechanism	NN
cord-104268-q1jx0n0l	442	7	that	that	WDT
cord-104268-q1jx0n0l	442	8	may	may	MD
cord-104268-q1jx0n0l	442	9	involve	involve	VB
cord-104268-q1jx0n0l	442	10	oligomerization	oligomerization	NN
cord-104268-q1jx0n0l	442	11	would	would	MD
cord-104268-q1jx0n0l	442	12	operate	operate	VB
cord-104268-q1jx0n0l	442	13	in	in	IN
cord-104268-q1jx0n0l	442	14	some	some	DT
cord-104268-q1jx0n0l	442	15	way	way	NN
cord-104268-q1jx0n0l	442	16	through	through	IN
cord-104268-q1jx0n0l	442	17	the	the	DT
cord-104268-q1jx0n0l	442	18	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	442	19	luminal	luminal	JJ
cord-104268-q1jx0n0l	442	20	domain	domain	NN
cord-104268-q1jx0n0l	442	21	.	.	.
cord-104268-q1jx0n0l	443	1	In	in	IN
cord-104268-q1jx0n0l	443	2	conclusion	conclusion	NN
cord-104268-q1jx0n0l	443	3	,	,	,
cord-104268-q1jx0n0l	443	4	our	-PRON-	PRP$
cord-104268-q1jx0n0l	443	5	observations	observation	NNS
cord-104268-q1jx0n0l	443	6	on	on	IN
cord-104268-q1jx0n0l	443	7	Kre2p	Kre2p	NNP
cord-104268-q1jx0n0l	443	8	targeting	target	VBG
cord-104268-q1jx0n0l	443	9	may	may	MD
cord-104268-q1jx0n0l	443	10	point	point	VB
cord-104268-q1jx0n0l	443	11	to	to	IN
cord-104268-q1jx0n0l	443	12	differences	difference	NNS
cord-104268-q1jx0n0l	443	13	in	in	IN
cord-104268-q1jx0n0l	443	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	443	15	localization	localization	NN
cord-104268-q1jx0n0l	443	16	mechanisms	mechanism	NNS
cord-104268-q1jx0n0l	443	17	between	between	IN
cord-104268-q1jx0n0l	443	18	yeast	yeast	NN
cord-104268-q1jx0n0l	443	19	and	and	CC
cord-104268-q1jx0n0l	443	20	animal	animal	NN
cord-104268-q1jx0n0l	443	21	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	443	22	or	or	CC
cord-104268-q1jx0n0l	443	23	could	could	MD
cord-104268-q1jx0n0l	443	24	indicate	indicate	VB
cord-104268-q1jx0n0l	443	25	that	that	IN
cord-104268-q1jx0n0l	443	26	multiple	multiple	JJ
cord-104268-q1jx0n0l	443	27	retention	retention	NN
cord-104268-q1jx0n0l	443	28	or	or	CC
cord-104268-q1jx0n0l	443	29	retrieval	retrieval	NN
cord-104268-q1jx0n0l	443	30	mechanisms	mechanism	NNS
cord-104268-q1jx0n0l	443	31	are	be	VBP
cord-104268-q1jx0n0l	443	32	used	use	VBN
cord-104268-q1jx0n0l	443	33	to	to	IN
cord-104268-q1jx0n0l	443	34	varying	vary	VBG
cord-104268-q1jx0n0l	443	35	extents	extent	NNS
cord-104268-q1jx0n0l	443	36	in	in	IN
cord-104268-q1jx0n0l	443	37	both	both	DT
cord-104268-q1jx0n0l	443	38	systems	system	NNS
cord-104268-q1jx0n0l	443	39	.	.	.
cord-104268-q1jx0n0l	444	1	Topography	topography	NN
cord-104268-q1jx0n0l	444	2	of	of	IN
cord-104268-q1jx0n0l	444	3	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	444	4	in	in	IN
cord-104268-q1jx0n0l	444	5	yeast	yeast	NN
cord-104268-q1jx0n0l	444	6	:	:	:
cord-104268-q1jx0n0l	444	7	characterization	characterization	NN
cord-104268-q1jx0n0l	444	8	of	of	IN
cord-104268-q1jx0n0l	444	9	GDP	gdp	NN
cord-104268-q1jx0n0l	444	10	-	-	HYPH
cord-104268-q1jx0n0l	444	11	mannose	mannose	NN
cord-104268-q1jx0n0l	444	12	transport	transport	NN
cord-104268-q1jx0n0l	444	13	and	and	CC
cord-104268-q1jx0n0l	444	14	lumenal	lumenal	JJ
cord-104268-q1jx0n0l	444	15	guanosine	guanosine	NN
cord-104268-q1jx0n0l	444	16	diphosphatase	diphosphatase	NN
cord-104268-q1jx0n0l	444	17	activities	activity	NNS
cord-104268-q1jx0n0l	444	18	in	in	IN
cord-104268-q1jx0n0l	444	19	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	444	20	-	-	HYPH
cord-104268-q1jx0n0l	444	21	like	like	JJ
cord-104268-q1jx0n0l	444	22	vesicles	vesicle	NNS
cord-104268-q1jx0n0l	444	23	Guanosine	Guanosine	NNP
cord-104268-q1jx0n0l	444	24	diphosphatase	diphosphatase	NN
cord-104268-q1jx0n0l	444	25	is	be	VBZ
cord-104268-q1jx0n0l	444	26	required	require	VBN
cord-104268-q1jx0n0l	444	27	for	for	IN
cord-104268-q1jx0n0l	444	28	protein	protein	NN
cord-104268-q1jx0n0l	444	29	and	and	CC
cord-104268-q1jx0n0l	444	30	sphingolipid	sphingolipid	NN
cord-104268-q1jx0n0l	444	31	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	444	32	in	in	IN
cord-104268-q1jx0n0l	444	33	the	the	DT
cord-104268-q1jx0n0l	444	34	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	444	35	lumen	lumen	NN
cord-104268-q1jx0n0l	444	36	of	of	IN
cord-104268-q1jx0n0l	444	37	Saccharomyces	saccharomyce	NNS
cord-104268-q1jx0n0l	444	38	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	444	39	PEP4	PEP4	NNP
cord-104268-q1jx0n0l	444	40	gene	gene	NN
cord-104268-q1jx0n0l	444	41	of	of	IN
cord-104268-q1jx0n0l	444	42	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	444	43	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	444	44	encodes	encode	VBZ
cord-104268-q1jx0n0l	444	45	proteinase	proteinase	NN
cord-104268-q1jx0n0l	444	46	A	A	NNP
cord-104268-q1jx0n0l	444	47	,	,	,
cord-104268-q1jx0n0l	444	48	a	a	DT
cord-104268-q1jx0n0l	444	49	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	444	50	enzyme	enzyme	NN
cord-104268-q1jx0n0l	444	51	required	require	VBN
cord-104268-q1jx0n0l	444	52	for	for	IN
cord-104268-q1jx0n0l	444	53	processing	processing	NN
cord-104268-q1jx0n0l	444	54	of	of	IN
cord-104268-q1jx0n0l	444	55	vacuolar	vacuolar	JJ
cord-104268-q1jx0n0l	444	56	precursors	precursor	NNS
cord-104268-q1jx0n0l	445	1	The	the	DT
cord-104268-q1jx0n0l	445	2	yeast	yeast	NN
cord-104268-q1jx0n0l	445	3	Ca2	ca2	CD
cord-104268-q1jx0n0l	445	4	+	+	CC
cord-104268-q1jx0n0l	446	1	-ATPase	-ATPase	NNP
cord-104268-q1jx0n0l	446	2	homologue	homologue	NNP
cord-104268-q1jx0n0l	446	3	,	,	,
cord-104268-q1jx0n0l	446	4	PMR1	PMR1	NNP
cord-104268-q1jx0n0l	446	5	is	be	VBZ
cord-104268-q1jx0n0l	446	6	required	require	VBN
cord-104268-q1jx0n0l	446	7	for	for	IN
cord-104268-q1jx0n0l	446	8	normal	normal	JJ
cord-104268-q1jx0n0l	446	9	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	446	10	function	function	NN
cord-104268-q1jx0n0l	446	11	and	and	CC
cord-104268-q1jx0n0l	446	12	localizes	localize	VBZ
cord-104268-q1jx0n0l	446	13	in	in	IN
cord-104268-q1jx0n0l	446	14	a	a	DT
cord-104268-q1jx0n0l	446	15	novel	novel	JJ
cord-104268-q1jx0n0l	446	16	Golgi	golgi	NN
cord-104268-q1jx0n0l	446	17	-	-	HYPH
cord-104268-q1jx0n0l	446	18	like	like	JJ
cord-104268-q1jx0n0l	446	19	distribution	distribution	NN
cord-104268-q1jx0n0l	446	20	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	446	21	retention	retention	NN
cord-104268-q1jx0n0l	446	22	of	of	IN
cord-104268-q1jx0n0l	446	23	a	a	DT
cord-104268-q1jx0n0l	446	24	trans	trans	JJ
cord-104268-q1jx0n0l	446	25	-	-	JJ
cord-104268-q1jx0n0l	446	26	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	446	27	membrane	membrane	NN
cord-104268-q1jx0n0l	446	28	protein	protein	NN
cord-104268-q1jx0n0l	446	29	.	.	.
cord-104268-q1jx0n0l	447	1	Galactosyltransferase	Galactosyltransferase	NNP
cord-104268-q1jx0n0l	447	2	requires	require	VBZ
cord-104268-q1jx0n0l	447	3	cysteine	cysteine	NN
cord-104268-q1jx0n0l	447	4	and	and	CC
cord-104268-q1jx0n0l	447	5	histidine	histidine	NN
cord-104268-q1jx0n0l	447	6	residues	residue	NNS
cord-104268-q1jx0n0l	447	7	within	within	IN
cord-104268-q1jx0n0l	447	8	the	the	DT
cord-104268-q1jx0n0l	447	9	membrane	membrane	NN
cord-104268-q1jx0n0l	447	10	-	-	HYPH
cord-104268-q1jx0n0l	447	11	anchoring	anchor	VBG
cord-104268-q1jx0n0l	447	12	domain	domain	NN
cord-104268-q1jx0n0l	447	13	Isolation	isolation	NN
cord-104268-q1jx0n0l	447	14	,	,	,
cord-104268-q1jx0n0l	447	15	characterization	characterization	NN
cord-104268-q1jx0n0l	447	16	,	,	,
cord-104268-q1jx0n0l	447	17	and	and	CC
cord-104268-q1jx0n0l	447	18	properties	property	NNS
cord-104268-q1jx0n0l	447	19	of	of	IN
cord-104268-q1jx0n0l	447	20	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	447	21	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	447	22	mnn	mnn	NNP
cord-104268-q1jx0n0l	447	23	mutants	mutant	NNS
cord-104268-q1jx0n0l	447	24	with	with	IN
cord-104268-q1jx0n0l	447	25	nonconditional	nonconditional	JJ
cord-104268-q1jx0n0l	447	26	protein	protein	NN
cord-104268-q1jx0n0l	447	27	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	447	28	defects	defect	NNS
cord-104268-q1jx0n0l	447	29	Revision	revision	NN
cord-104268-q1jx0n0l	447	30	of	of	IN
cord-104268-q1jx0n0l	447	31	the	the	DT
cord-104268-q1jx0n0l	447	32	oligosaccharide	oligosaccharide	NN
cord-104268-q1jx0n0l	447	33	structures	structure	NNS
cord-104268-q1jx0n0l	447	34	of	of	IN
cord-104268-q1jx0n0l	447	35	yeast	yeast	NN
cord-104268-q1jx0n0l	447	36	carboxypeptidase	carboxypeptidase	NN
cord-104268-q1jx0n0l	447	37	Y	Y	NNP
cord-104268-q1jx0n0l	447	38	Localization	Localization	NNP
cord-104268-q1jx0n0l	447	39	of	of	IN
cord-104268-q1jx0n0l	447	40	Sed5	Sed5	NNP
cord-104268-q1jx0n0l	447	41	,	,	,
cord-104268-q1jx0n0l	447	42	a	a	DT
cord-104268-q1jx0n0l	447	43	putative	putative	JJ
cord-104268-q1jx0n0l	447	44	vesicle	vesicle	NN
cord-104268-q1jx0n0l	447	45	targeting	target	VBG
cord-104268-q1jx0n0l	447	46	molecule	molecule	NN
cord-104268-q1jx0n0l	447	47	,	,	,
cord-104268-q1jx0n0l	447	48	to	to	IN
cord-104268-q1jx0n0l	447	49	the	the	DT
cord-104268-q1jx0n0l	447	50	eis	eis	NNP
cord-104268-q1jx0n0l	447	51	-	-	HYPH
cord-104268-q1jx0n0l	447	52	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	447	53	network	network	NN
cord-104268-q1jx0n0l	447	54	involves	involve	VBZ
cord-104268-q1jx0n0l	447	55	both	both	CC
cord-104268-q1jx0n0l	447	56	its	-PRON-	PRP$
cord-104268-q1jx0n0l	447	57	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	447	58	and	and	CC
cord-104268-q1jx0n0l	447	59	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	447	60	domains	domain	NNS
cord-104268-q1jx0n0l	447	61	Yeast	Yeast	NNP
cord-104268-q1jx0n0l	447	62	KRE	KRE	NNP
cord-104268-q1jx0n0l	447	63	genes	gene	NNS
cord-104268-q1jx0n0l	447	64	provide	provide	VBP
cord-104268-q1jx0n0l	447	65	evidence	evidence	NN
cord-104268-q1jx0n0l	447	66	for	for	IN
cord-104268-q1jx0n0l	447	67	a	a	DT
cord-104268-q1jx0n0l	447	68	pathway	pathway	NN
cord-104268-q1jx0n0l	447	69	of	of	IN
cord-104268-q1jx0n0l	447	70	cell	cell	NN
cord-104268-q1jx0n0l	447	71	wall	wall	NN
cord-104268-q1jx0n0l	447	72	[	[	-LRB-
cord-104268-q1jx0n0l	447	73	3-glucan	3-glucan	CD
cord-104268-q1jx0n0l	447	74	assembly	assembly	NN
cord-104268-q1jx0n0l	447	75	TGN38	TGN38	NNS
cord-104268-q1jx0n0l	447	76	is	be	VBZ
cord-104268-q1jx0n0l	447	77	maintained	maintain	VBN
cord-104268-q1jx0n0l	447	78	in	in	IN
cord-104268-q1jx0n0l	447	79	the	the	DT
cord-104268-q1jx0n0l	447	80	trans	trans	NNP
cord-104268-q1jx0n0l	447	81	-	-	NNP
cord-104268-q1jx0n0l	447	82	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	447	83	network	network	NN
cord-104268-q1jx0n0l	447	84	by	by	IN
cord-104268-q1jx0n0l	447	85	a	a	DT
cord-104268-q1jx0n0l	447	86	tyrosine	tyrosine	NN
cord-104268-q1jx0n0l	447	87	-	-	HYPH
cord-104268-q1jx0n0l	447	88	containing	contain	VBG
cord-104268-q1jx0n0l	447	89	motif	motif	NN
cord-104268-q1jx0n0l	447	90	in	in	IN
cord-104268-q1jx0n0l	447	91	the	the	DT
cord-104268-q1jx0n0l	447	92	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	447	93	domain	domain	NN
cord-104268-q1jx0n0l	448	1	Secl5	Secl5	NNP
cord-104268-q1jx0n0l	449	1	protein	protein	NN
cord-104268-q1jx0n0l	449	2	,	,	,
cord-104268-q1jx0n0l	449	3	an	an	DT
cord-104268-q1jx0n0l	449	4	essential	essential	JJ
cord-104268-q1jx0n0l	449	5	component	component	NN
cord-104268-q1jx0n0l	449	6	of	of	IN
cord-104268-q1jx0n0l	449	7	the	the	DT
cord-104268-q1jx0n0l	449	8	exocytic	exocytic	JJ
cord-104268-q1jx0n0l	449	9	apparatus	apparatus	NN
cord-104268-q1jx0n0l	449	10	,	,	,
cord-104268-q1jx0n0l	449	11	is	be	VBZ
cord-104268-q1jx0n0l	449	12	associated	associate	VBN
cord-104268-q1jx0n0l	449	13	with	with	IN
cord-104268-q1jx0n0l	449	14	the	the	DT
cord-104268-q1jx0n0l	449	15	plasma	plasma	NN
cord-104268-q1jx0n0l	449	16	membrane	membrane	NN
cord-104268-q1jx0n0l	449	17	and	and	CC
cord-104268-q1jx0n0l	449	18	with	with	IN
cord-104268-q1jx0n0l	449	19	a	a	DT
cord-104268-q1jx0n0l	449	20	soluble	soluble	JJ
cord-104268-q1jx0n0l	449	21	19.5S	19.5s	CD
cord-104268-q1jx0n0l	449	22	particle	particle	NN
cord-104268-q1jx0n0l	449	23	The	the	DT
cord-104268-q1jx0n0l	449	24	K1	K1	NNP
cord-104268-q1jx0n0l	449	25	killer	killer	NN
cord-104268-q1jx0n0l	449	26	toxin	toxin	NN
cord-104268-q1jx0n0l	449	27	:	:	:
cord-104268-q1jx0n0l	449	28	molecular	molecular	JJ
cord-104268-q1jx0n0l	449	29	and	and	CC
cord-104268-q1jx0n0l	449	30	genetic	genetic	JJ
cord-104268-q1jx0n0l	449	31	applications	application	NNS
cord-104268-q1jx0n0l	449	32	to	to	IN
cord-104268-q1jx0n0l	449	33	secretion	secretion	NN
cord-104268-q1jx0n0l	449	34	and	and	CC
cord-104268-q1jx0n0l	449	35	cell	cell	NN
cord-104268-q1jx0n0l	449	36	surface	surface	NN
cord-104268-q1jx0n0l	449	37	assembly	assembly	NN
cord-104268-q1jx0n0l	449	38	.	.	.
cord-104268-q1jx0n0l	450	1	In	in	IN
cord-104268-q1jx0n0l	450	2	Molecular	Molecular	NNP
cord-104268-q1jx0n0l	450	3	Genetics	Genetics	NNP
cord-104268-q1jx0n0l	450	4	of	of	IN
cord-104268-q1jx0n0l	450	5	Yeast	Yeast	NNP
cord-104268-q1jx0n0l	450	6	:	:	:
cord-104268-q1jx0n0l	450	7	A	a	DT
cord-104268-q1jx0n0l	450	8	Practical	practical	JJ
cord-104268-q1jx0n0l	450	9	Approach	Approach	NNP
cord-104268-q1jx0n0l	450	10	Immunoisolation	Immunoisolation	NNP
cord-104268-q1jx0n0l	450	11	of	of	IN
cord-104268-q1jx0n0l	450	12	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	450	13	-	-	HYPH
cord-104268-q1jx0n0l	450	14	containing	contain	VBG
cord-104268-q1jx0n0l	450	15	organelles	organelle	NNS
cord-104268-q1jx0n0l	450	16	from	from	IN
cord-104268-q1jx0n0l	450	17	yeast	yeast	NN
cord-104268-q1jx0n0l	450	18	demonstrates	demonstrate	VBZ
cord-104268-q1jx0n0l	450	19	colocalisation	colocalisation	NN
cord-104268-q1jx0n0l	450	20	of	of	IN
cord-104268-q1jx0n0l	450	21	three	three	CD
cord-104268-q1jx0n0l	450	22	processing	processing	NN
cord-104268-q1jx0n0l	450	23	proteinases	proteinase	NNS
cord-104268-q1jx0n0l	450	24	to	to	IN
cord-104268-q1jx0n0l	450	25	a	a	DT
cord-104268-q1jx0n0l	450	26	single	single	JJ
cord-104268-q1jx0n0l	450	27	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	450	28	compartment	compartment	NN
cord-104268-q1jx0n0l	451	1	The	the	DT
cord-104268-q1jx0n0l	451	2	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	451	3	and	and	CC
cord-104268-q1jx0n0l	451	4	flanking	flanking	JJ
cord-104268-q1jx0n0l	451	5	sequences	sequence	NNS
cord-104268-q1jx0n0l	451	6	of	of	IN
cord-104268-q1jx0n0l	451	7	131,2-N	131,2-n	CD
cord-104268-q1jx0n0l	451	8	-	-	HYPH
cord-104268-q1jx0n0l	451	9	acetylglucosaminyltransferase	acetylglucosaminyltransferase	NN
cord-104268-q1jx0n0l	452	1	I	-PRON-	PRP
cord-104268-q1jx0n0l	452	2	specify	specify	VBP
cord-104268-q1jx0n0l	452	3	medial	medial	JJ
cord-104268-q1jx0n0l	452	4	-	-	HYPH
cord-104268-q1jx0n0l	452	5	Golgi	golgi	NN
cord-104268-q1jx0n0l	452	6	localization	localization	NN
cord-104268-q1jx0n0l	452	7	Medial	Medial	NNP
cord-104268-q1jx0n0l	452	8	-	-	HYPH
cord-104268-q1jx0n0l	452	9	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	452	10	retention	retention	NN
cord-104268-q1jx0n0l	452	11	of	of	IN
cord-104268-q1jx0n0l	452	12	N	N	NNP
cord-104268-q1jx0n0l	452	13	-	-	NN
cord-104268-q1jx0n0l	452	14	acetylglucosaminyltransferase	acetylglucosaminyltransferase	NNP
cord-104268-q1jx0n0l	453	1	I	-PRON-	PRP
cord-104268-q1jx0n0l	454	1	A	a	DT
cord-104268-q1jx0n0l	454	2	12.8	12.8	CD
cord-104268-q1jx0n0l	454	3	kB	kb	CD
cord-104268-q1jx0n0l	454	4	segment	segment	NN
cord-104268-q1jx0n0l	454	5	on	on	IN
cord-104268-q1jx0n0l	454	6	the	the	DT
cord-104268-q1jx0n0l	454	7	right	right	JJ
cord-104268-q1jx0n0l	454	8	arm	arm	NN
cord-104268-q1jx0n0l	454	9	of	of	IN
cord-104268-q1jx0n0l	454	10	chromosome	chromosome	NN
cord-104268-q1jx0n0l	454	11	II	II	NNP
cord-104268-q1jx0n0l	454	12	from	from	IN
cord-104268-q1jx0n0l	454	13	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	454	14	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	454	15	including	include	VBG
cord-104268-q1jx0n0l	454	16	part	part	NN
cord-104268-q1jx0n0l	454	17	of	of	IN
cord-104268-q1jx0n0l	454	18	the	the	DT
cord-104268-q1jx0n0l	454	19	DUR12	DUR12	NNP
cord-104268-q1jx0n0l	454	20	gene	gene	NN
cord-104268-q1jx0n0l	454	21	,	,	,
cord-104268-q1jx0n0l	454	22	contains	contain	VBZ
cord-104268-q1jx0n0l	454	23	five	five	CD
cord-104268-q1jx0n0l	454	24	putative	putative	JJ
cord-104268-q1jx0n0l	454	25	new	new	JJ
cord-104268-q1jx0n0l	454	26	genes	gene	NNS
cord-104268-q1jx0n0l	454	27	K1	K1	NNP
cord-104268-q1jx0n0l	454	28	killer	killer	NN
cord-104268-q1jx0n0l	454	29	toxin	toxin	NN
cord-104268-q1jx0n0l	454	30	,	,	,
cord-104268-q1jx0n0l	454	31	a	a	DT
cord-104268-q1jx0n0l	454	32	pore	pore	NN
cord-104268-q1jx0n0l	454	33	-	-	HYPH
cord-104268-q1jx0n0l	454	34	forming	form	VBG
cord-104268-q1jx0n0l	454	35	protein	protein	NN
cord-104268-q1jx0n0l	454	36	from	from	IN
cord-104268-q1jx0n0l	454	37	yeast	yeast	NN
cord-104268-q1jx0n0l	455	1	The	the	DT
cord-104268-q1jx0n0l	455	2	functioning	functioning	NN
cord-104268-q1jx0n0l	455	3	of	of	IN
cord-104268-q1jx0n0l	455	4	the	the	DT
cord-104268-q1jx0n0l	455	5	yeast	yeast	NN
cord-104268-q1jx0n0l	455	6	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	455	7	apparatus	apparatus	NN
cord-104268-q1jx0n0l	455	8	requires	require	VBZ
cord-104268-q1jx0n0l	455	9	an	an	DT
cord-104268-q1jx0n0l	455	10	ER	ER	NNP
cord-104268-q1jx0n0l	455	11	protein	protein	NN
cord-104268-q1jx0n0l	455	12	encoded	encode	VBN
cord-104268-q1jx0n0l	455	13	by	by	IN
cord-104268-q1jx0n0l	455	14	ANP1	ANP1	NNP
cord-104268-q1jx0n0l	455	15	,	,	,
cord-104268-q1jx0n0l	455	16	a	a	DT
cord-104268-q1jx0n0l	455	17	member	member	NN
cord-104268-q1jx0n0l	455	18	of	of	IN
cord-104268-q1jx0n0l	455	19	a	a	DT
cord-104268-q1jx0n0l	455	20	new	new	JJ
cord-104268-q1jx0n0l	455	21	family	family	NN
cord-104268-q1jx0n0l	455	22	of	of	IN
cord-104268-q1jx0n0l	455	23	genes	gene	NNS
cord-104268-q1jx0n0l	455	24	affecting	affect	VBG
cord-104268-q1jx0n0l	455	25	the	the	DT
cord-104268-q1jx0n0l	455	26	secretory	secretory	JJ
cord-104268-q1jx0n0l	455	27	pathway	pathway	NN
cord-104268-q1jx0n0l	455	28	The	the	DT
cord-104268-q1jx0n0l	455	29	signal	signal	NN
cord-104268-q1jx0n0l	455	30	anchor	anchor	NN
cord-104268-q1jx0n0l	455	31	and	and	CC
cord-104268-q1jx0n0l	455	32	stem	stem	NN
cord-104268-q1jx0n0l	455	33	regions	region	NNS
cord-104268-q1jx0n0l	455	34	of	of	IN
cord-104268-q1jx0n0l	455	35	the	the	DT
cord-104268-q1jx0n0l	455	36	[	[	-LRB-
cord-104268-q1jx0n0l	455	37	3-galactoside	3-galactoside	CD
cord-104268-q1jx0n0l	456	1	~x2,6-sialyltransferase	~x2,6-sialyltransferase	CD
cord-104268-q1jx0n0l	456	2	may	may	MD
cord-104268-q1jx0n0l	456	3	each	each	DT
cord-104268-q1jx0n0l	456	4	act	act	VB
cord-104268-q1jx0n0l	456	5	to	to	TO
cord-104268-q1jx0n0l	456	6	localize	localize	VB
cord-104268-q1jx0n0l	456	7	the	the	DT
cord-104268-q1jx0n0l	456	8	enzyme	enzyme	NN
cord-104268-q1jx0n0l	456	9	to	to	IN
cord-104268-q1jx0n0l	456	10	the	the	DT
cord-104268-q1jx0n0l	456	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	456	12	apparatus	apparatus	NN
cord-104268-q1jx0n0l	456	13	Characterization	characterization	NN
cord-104268-q1jx0n0l	456	14	of	of	IN
cord-104268-q1jx0n0l	456	15	the	the	DT
cord-104268-q1jx0n0l	456	16	yeast	yeast	NN
cord-104268-q1jx0n0l	456	17	KEX1	KEX1	NNP
cord-104268-q1jx0n0l	456	18	gene	gene	NN
cord-104268-q1jx0n0l	456	19	product	product	NN
cord-104268-q1jx0n0l	456	20	:	:	:
cord-104268-q1jx0n0l	456	21	a	a	DT
cord-104268-q1jx0n0l	456	22	carboxypeptidase	carboxypeptidase	NN
cord-104268-q1jx0n0l	456	23	involved	involve	VBN
cord-104268-q1jx0n0l	456	24	in	in	IN
cord-104268-q1jx0n0l	456	25	processing	processing	NN
cord-104268-q1jx0n0l	456	26	of	of	IN
cord-104268-q1jx0n0l	456	27	secreted	secrete	VBN
cord-104268-q1jx0n0l	456	28	precursor	precursor	NN
cord-104268-q1jx0n0l	456	29	proteins	protein	NNS
cord-104268-q1jx0n0l	456	30	Yeast	yeast	VBP
cord-104268-q1jx0n0l	456	31	Kexlp	Kexlp	NNP
cord-104268-q1jx0n0l	456	32	is	be	VBZ
cord-104268-q1jx0n0l	456	33	a	a	DT
cord-104268-q1jx0n0l	456	34	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	456	35	-	-	HYPH
cord-104268-q1jx0n0l	456	36	associated	associate	VBN
cord-104268-q1jx0n0l	456	37	membrane	membrane	NN
cord-104268-q1jx0n0l	456	38	protein	protein	NN
cord-104268-q1jx0n0l	456	39	:	:	:
cord-104268-q1jx0n0l	457	1	deletions	deletion	NNS
cord-104268-q1jx0n0l	457	2	in	in	IN
cord-104268-q1jx0n0l	457	3	a	a	DT
cord-104268-q1jx0n0l	457	4	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	457	5	targeting	targeting	NN
cord-104268-q1jx0n0l	457	6	domain	domain	NN
cord-104268-q1jx0n0l	457	7	result	result	NN
cord-104268-q1jx0n0l	457	8	in	in	IN
cord-104268-q1jx0n0l	457	9	mislocalization	mislocalization	NN
cord-104268-q1jx0n0l	457	10	to	to	IN
cord-104268-q1jx0n0l	457	11	the	the	DT
cord-104268-q1jx0n0l	457	12	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	457	13	membrane	membrane	NN
cord-104268-q1jx0n0l	457	14	Specific	specific	JJ
cord-104268-q1jx0n0l	457	15	sequences	sequence	NNS
cord-104268-q1jx0n0l	457	16	in	in	IN
cord-104268-q1jx0n0l	457	17	the	the	DT
cord-104268-q1jx0n0l	457	18	signal	signal	NN
cord-104268-q1jx0n0l	457	19	anchor	anchor	NN
cord-104268-q1jx0n0l	457	20	of	of	IN
cord-104268-q1jx0n0l	457	21	the	the	DT
cord-104268-q1jx0n0l	457	22	13-galactoside	13-galactoside	CD
cord-104268-q1jx0n0l	457	23	a-2,6-sialyltransferase	a-2,6-sialyltransferase	NNP
cord-104268-q1jx0n0l	457	24	are	be	VBP
cord-104268-q1jx0n0l	457	25	not	not	RB
cord-104268-q1jx0n0l	457	26	essential	essential	JJ
cord-104268-q1jx0n0l	457	27	for	for	IN
cord-104268-q1jx0n0l	457	28	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	29	retention	retention	NN
cord-104268-q1jx0n0l	457	30	Yeast	Yeast	NNP
cord-104268-q1jx0n0l	457	31	KEX1	KEX1	NNP
cord-104268-q1jx0n0l	457	32	gene	gene	NN
cord-104268-q1jx0n0l	457	33	encodes	encode	VBZ
cord-104268-q1jx0n0l	457	34	a	a	DT
cord-104268-q1jx0n0l	457	35	putative	putative	JJ
cord-104268-q1jx0n0l	457	36	protease	protease	NN
cord-104268-q1jx0n0l	457	37	with	with	IN
cord-104268-q1jx0n0l	457	38	a	a	DT
cord-104268-q1jx0n0l	457	39	carboxypeptidase	carboxypeptidase	NN
cord-104268-q1jx0n0l	457	40	B	b	NN
cord-104268-q1jx0n0l	457	41	-	-	HYPH
cord-104268-q1jx0n0l	457	42	like	like	JJ
cord-104268-q1jx0n0l	457	43	function	function	NN
cord-104268-q1jx0n0l	457	44	involved	involve	VBN
cord-104268-q1jx0n0l	457	45	in	in	IN
cord-104268-q1jx0n0l	457	46	killer	killer	NN
cord-104268-q1jx0n0l	457	47	toxin	toxin	NN
cord-104268-q1jx0n0l	457	48	and	and	CC
cord-104268-q1jx0n0l	457	49	alpha	alpha	NN
cord-104268-q1jx0n0l	457	50	factor	factor	NN
cord-104268-q1jx0n0l	457	51	precursor	precursor	NN
cord-104268-q1jx0n0l	457	52	processing	process	VBG
cord-104268-q1jx0n0l	457	53	Functional	functional	JJ
cord-104268-q1jx0n0l	457	54	compartments	compartment	NNS
cord-104268-q1jx0n0l	457	55	of	of	IN
cord-104268-q1jx0n0l	457	56	the	the	DT
cord-104268-q1jx0n0l	457	57	yeast	yeast	NN
cord-104268-q1jx0n0l	457	58	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	59	apparatus	apparatus	NN
cord-104268-q1jx0n0l	457	60	are	be	VBP
cord-104268-q1jx0n0l	457	61	defined	define	VBN
cord-104268-q1jx0n0l	457	62	by	by	IN
cord-104268-q1jx0n0l	457	63	the	the	DT
cord-104268-q1jx0n0l	457	64	see7	see7	NNP
cord-104268-q1jx0n0l	457	65	mutation	mutation	NN
cord-104268-q1jx0n0l	457	66	Localization	Localization	NNP
cord-104268-q1jx0n0l	457	67	of	of	IN
cord-104268-q1jx0n0l	457	68	components	component	NNS
cord-104268-q1jx0n0l	457	69	involved	involve	VBN
cord-104268-q1jx0n0l	457	70	in	in	IN
cord-104268-q1jx0n0l	457	71	protein	protein	NN
cord-104268-q1jx0n0l	457	72	transport	transport	NN
cord-104268-q1jx0n0l	457	73	and	and	CC
cord-104268-q1jx0n0l	457	74	processing	process	VBG
cord-104268-q1jx0n0l	457	75	through	through	IN
cord-104268-q1jx0n0l	457	76	the	the	DT
cord-104268-q1jx0n0l	457	77	yeast	yeast	NN
cord-104268-q1jx0n0l	457	78	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	79	apparatus	apparatus	NN
cord-104268-q1jx0n0l	457	80	Signal	signal	NN
cord-104268-q1jx0n0l	457	81	-	-	HYPH
cord-104268-q1jx0n0l	457	82	mediated	mediate	VBN
cord-104268-q1jx0n0l	457	83	retrieval	retrieval	NN
cord-104268-q1jx0n0l	457	84	of	of	IN
cord-104268-q1jx0n0l	457	85	a	a	DT
cord-104268-q1jx0n0l	457	86	membrane	membrane	NN
cord-104268-q1jx0n0l	457	87	protein	protein	NN
cord-104268-q1jx0n0l	457	88	from	from	IN
cord-104268-q1jx0n0l	457	89	the	the	DT
cord-104268-q1jx0n0l	457	90	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	91	to	to	IN
cord-104268-q1jx0n0l	457	92	the	the	DT
cord-104268-q1jx0n0l	457	93	ER	ER	NNP
cord-104268-q1jx0n0l	457	94	in	in	IN
cord-104268-q1jx0n0l	457	95	yeast	yeast	NN
cord-104268-q1jx0n0l	457	96	Studies	Studies	NNPS
cord-104268-q1jx0n0l	457	97	on	on	IN
cord-104268-q1jx0n0l	457	98	the	the	DT
cord-104268-q1jx0n0l	457	99	transformation	transformation	NN
cord-104268-q1jx0n0l	457	100	of	of	IN
cord-104268-q1jx0n0l	457	101	intact	intact	JJ
cord-104268-q1jx0n0l	457	102	yeast	yeast	NN
cord-104268-q1jx0n0l	457	103	cells	cell	NNS
cord-104268-q1jx0n0l	457	104	by	by	IN
cord-104268-q1jx0n0l	457	105	the	the	DT
cord-104268-q1jx0n0l	457	106	LiAc	LiAc	NNP
cord-104268-q1jx0n0l	457	107	/	/	SYM
cord-104268-q1jx0n0l	457	108	SS	SS	NNP
cord-104268-q1jx0n0l	457	109	-	-	HYPH
cord-104268-q1jx0n0l	457	110	DNA	DNA	NNP
cord-104268-q1jx0n0l	457	111	/	/	SYM
cord-104268-q1jx0n0l	457	112	PEG	PEG	NNP
cord-104268-q1jx0n0l	457	113	procedure	procedure	NN
cord-104268-q1jx0n0l	457	114	Targeting	targeting	NN
cord-104268-q1jx0n0l	457	115	of	of	IN
cord-104268-q1jx0n0l	457	116	proteins	protein	NNS
cord-104268-q1jx0n0l	457	117	to	to	IN
cord-104268-q1jx0n0l	457	118	the	the	DT
cord-104268-q1jx0n0l	457	119	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	120	apparatus	apparatus	NN
cord-104268-q1jx0n0l	457	121	Compartmental	Compartmental	NNP
cord-104268-q1jx0n0l	457	122	organization	organization	NN
cord-104268-q1jx0n0l	457	123	of	of	IN
cord-104268-q1jx0n0l	457	124	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	457	125	-	-	HYPH
cord-104268-q1jx0n0l	457	126	specific	specific	JJ
cord-104268-q1jx0n0l	457	127	protein	protein	NN
cord-104268-q1jx0n0l	457	128	modification	modification	NN
cord-104268-q1jx0n0l	457	129	and	and	CC
cord-104268-q1jx0n0l	457	130	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	457	131	protein	protein	NN
cord-104268-q1jx0n0l	457	132	sorting	sort	VBG
cord-104268-q1jx0n0l	457	133	events	event	NNS
cord-104268-q1jx0n0l	457	134	defined	define	VBN
cord-104268-q1jx0n0l	457	135	in	in	IN
cord-104268-q1jx0n0l	457	136	yeast	yeast	NN
cord-104268-q1jx0n0l	457	137	sec18	sec18	.
cord-104268-q1jx0n0l	458	1	(	(	-LRB-
cord-104268-q1jx0n0l	458	2	NSF	NSF	NNP
cord-104268-q1jx0n0l	458	3	)	)	-RRB-
cord-104268-q1jx0n0l	458	4	mutant	mutant	JJ
cord-104268-q1jx0n0l	458	5	Clathrin	Clathrin	NNP
cord-104268-q1jx0n0l	458	6	-	-	HYPH
cord-104268-q1jx0n0l	458	7	dependent	dependent	JJ
cord-104268-q1jx0n0l	458	8	localization	localization	NN
cord-104268-q1jx0n0l	458	9	of	of	IN
cord-104268-q1jx0n0l	458	10	a-1,3	a-1,3	NN
cord-104268-q1jx0n0l	458	11	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	458	12	to	to	IN
cord-104268-q1jx0n0l	458	13	the	the	DT
cord-104268-q1jx0n0l	458	14	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	458	15	complex	complex	NN
cord-104268-q1jx0n0l	458	16	of	of	IN
cord-104268-q1jx0n0l	458	17	S.	S.	NNP
cord-104268-q1jx0n0l	458	18	cerevisiae	cerevisiae	NN
cord-104268-q1jx0n0l	459	1	The	the	DT
cord-104268-q1jx0n0l	459	2	ER	ER	NNP
cord-104268-q1jx0n0l	459	3	-	-	HYPH
cord-104268-q1jx0n0l	459	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	459	5	intermediate	intermediate	JJ
cord-104268-q1jx0n0l	459	6	compartment	compartment	NN
cord-104268-q1jx0n0l	459	7	Glycosylation	Glycosylation	NNP
cord-104268-q1jx0n0l	459	8	in	in	IN
cord-104268-q1jx0n0l	459	9	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	459	10	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	459	11	:	:	:
cord-104268-q1jx0n0l	460	1	cloning	clone	VBG
cord-104268-q1jx0n0l	460	2	and	and	CC
cord-104268-q1jx0n0l	460	3	characterization	characterization	NN
cord-104268-q1jx0n0l	460	4	of	of	IN
cord-104268-q1jx0n0l	460	5	an	an	DT
cord-104268-q1jx0n0l	460	6	a	a	DT
cord-104268-q1jx0n0l	460	7	-	-	HYPH
cord-104268-q1jx0n0l	460	8	l,2-mannosyltransferase	l,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	460	9	structural	structural	JJ
cord-104268-q1jx0n0l	460	10	gene	gene	NN
cord-104268-q1jx0n0l	460	11	Yeast	yeast	NN
cord-104268-q1jx0n0l	460	12	glycoprotein	glycoprotein	NN
cord-104268-q1jx0n0l	460	13	biosynthesis	biosynthesis	NN
cord-104268-q1jx0n0l	460	14	:	:	:
cord-104268-q1jx0n0l	461	1	MNT1	MNT1	NNP
cord-104268-q1jx0n0l	461	2	encodes	encode	VBZ
cord-104268-q1jx0n0l	461	3	an	an	DT
cord-104268-q1jx0n0l	461	4	c~-l,2-mannosyltransferase	c~-l,2-mannosyltransferase	NN
cord-104268-q1jx0n0l	461	5	involved	involve	VBN
cord-104268-q1jx0n0l	461	6	in	in	IN
cord-104268-q1jx0n0l	461	7	O	o	NN
cord-104268-q1jx0n0l	461	8	-	-	HYPH
cord-104268-q1jx0n0l	461	9	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	461	10	Glycoprotein	Glycoprotein	NNP
cord-104268-q1jx0n0l	461	11	biosynthesis	biosynthesis	NN
cord-104268-q1jx0n0l	461	12	in	in	IN
cord-104268-q1jx0n0l	461	13	yeast	yeast	NN
cord-104268-q1jx0n0l	461	14	Yeast	Yeast	NNP
cord-104268-q1jx0n0l	461	15	/	/	SYM
cord-104268-q1jx0n0l	461	16	E.	E.	NNP
cord-104268-q1jx0n0l	461	17	coli	coli	NN
cord-104268-q1jx0n0l	461	18	shuttle	shuttle	NN
cord-104268-q1jx0n0l	461	19	vectors	vector	NNS
cord-104268-q1jx0n0l	461	20	with	with	IN
cord-104268-q1jx0n0l	461	21	multiple	multiple	JJ
cord-104268-q1jx0n0l	461	22	unique	unique	JJ
cord-104268-q1jx0n0l	461	23	restriction	restriction	NN
cord-104268-q1jx0n0l	461	24	sites	site	NNS
cord-104268-q1jx0n0l	461	25	Yeast	yeast	VBP
cord-104268-q1jx0n0l	461	26	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	461	27	defines	define	VBZ
cord-104268-q1jx0n0l	461	28	a	a	DT
cord-104268-q1jx0n0l	461	29	new	new	JJ
cord-104268-q1jx0n0l	461	30	gene	gene	NN
cord-104268-q1jx0n0l	461	31	family	family	NN
cord-104268-q1jx0n0l	461	32	encoding	encode	VBG
cord-104268-q1jx0n0l	461	33	probable	probable	JJ
cord-104268-q1jx0n0l	461	34	secretory	secretory	JJ
cord-104268-q1jx0n0l	461	35	proteins	protein	NNS
cord-104268-q1jx0n0l	461	36	,	,	,
cord-104268-q1jx0n0l	461	37	and	and	CC
cord-104268-q1jx0n0l	461	38	is	be	VBZ
cord-104268-q1jx0n0l	461	39	required	require	VBN
cord-104268-q1jx0n0l	461	40	for	for	IN
cord-104268-q1jx0n0l	461	41	the	the	DT
cord-104268-q1jx0n0l	461	42	correct	correct	JJ
cord-104268-q1jx0n0l	461	43	N	n	NN
cord-104268-q1jx0n0l	461	44	-	-	HYPH
cord-104268-q1jx0n0l	461	45	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	461	46	of	of	IN
cord-104268-q1jx0n0l	461	47	proteins	protein	NNS
cord-104268-q1jx0n0l	462	1	Vma21p	Vma21p	NNP
cord-104268-q1jx0n0l	462	2	is	be	VBZ
cord-104268-q1jx0n0l	462	3	a	a	DT
cord-104268-q1jx0n0l	462	4	yeast	yeast	NN
cord-104268-q1jx0n0l	462	5	membrane	membrane	NN
cord-104268-q1jx0n0l	462	6	protein	protein	NN
cord-104268-q1jx0n0l	462	7	retained	retain	VBN
cord-104268-q1jx0n0l	462	8	in	in	IN
cord-104268-q1jx0n0l	462	9	the	the	DT
cord-104268-q1jx0n0l	462	10	endoplasmic	endoplasmic	NN
cord-104268-q1jx0n0l	462	11	reticulum	reticulum	NN
cord-104268-q1jx0n0l	462	12	by	by	IN
cord-104268-q1jx0n0l	462	13	a	a	DT
cord-104268-q1jx0n0l	462	14	di	di	NN
cord-104268-q1jx0n0l	462	15	-	-	HYPH
cord-104268-q1jx0n0l	462	16	lysine	lysine	JJ
cord-104268-q1jx0n0l	462	17	motif	motif	NN
cord-104268-q1jx0n0l	462	18	and	and	CC
cord-104268-q1jx0n0l	462	19	is	be	VBZ
cord-104268-q1jx0n0l	462	20	required	require	VBN
cord-104268-q1jx0n0l	462	21	for	for	IN
cord-104268-q1jx0n0l	462	22	the	the	DT
cord-104268-q1jx0n0l	462	23	assembly	assembly	NN
cord-104268-q1jx0n0l	462	24	of	of	IN
cord-104268-q1jx0n0l	462	25	the	the	DT
cord-104268-q1jx0n0l	462	26	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	462	27	H+-ATPase	H+-ATPase	NNP
cord-104268-q1jx0n0l	462	28	complex	complex	JJ
cord-104268-q1jx0n0l	462	29	Localization	localization	NN
cord-104268-q1jx0n0l	462	30	of	of	IN
cord-104268-q1jx0n0l	462	31	TGN38	TGN38	NNS
cord-104268-q1jx0n0l	462	32	to	to	IN
cord-104268-q1jx0n0l	462	33	the	the	DT
cord-104268-q1jx0n0l	462	34	trans	trans	NNP
cord-104268-q1jx0n0l	462	35	-	-	NNP
cord-104268-q1jx0n0l	462	36	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	462	37	network	network	NN
cord-104268-q1jx0n0l	462	38	:	:	:
cord-104268-q1jx0n0l	462	39	involvement	involvement	NN
cord-104268-q1jx0n0l	462	40	of	of	IN
cord-104268-q1jx0n0l	462	41	a	a	DT
cord-104268-q1jx0n0l	462	42	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	462	43	tyrosine	tyrosine	NN
cord-104268-q1jx0n0l	462	44	--	--	:
cord-104268-q1jx0n0l	462	45	containiug	containiug	NNP
cord-104268-q1jx0n0l	462	46	sequence	sequence	NN
cord-104268-q1jx0n0l	462	47	Assembly	Assembly	NNP
cord-104268-q1jx0n0l	462	48	and	and	CC
cord-104268-q1jx0n0l	462	49	targeting	targeting	NN
cord-104268-q1jx0n0l	462	50	of	of	IN
cord-104268-q1jx0n0l	462	51	peripheral	peripheral	JJ
cord-104268-q1jx0n0l	462	52	and	and	CC
cord-104268-q1jx0n0l	462	53	integral	integral	JJ
cord-104268-q1jx0n0l	462	54	membrane	membrane	NN
cord-104268-q1jx0n0l	462	55	subunits	subunit	NNS
cord-104268-q1jx0n0l	462	56	of	of	IN
cord-104268-q1jx0n0l	462	57	the	the	DT
cord-104268-q1jx0n0l	462	58	yeast	yeast	NN
cord-104268-q1jx0n0l	462	59	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	463	1	H+-ATPase	h+-atpase	NN
cord-104268-q1jx0n0l	464	1	Structural	structural	JJ
cord-104268-q1jx0n0l	464	2	characteristics	characteristic	NNS
cord-104268-q1jx0n0l	464	3	of	of	IN
cord-104268-q1jx0n0l	464	4	the	the	DT
cord-104268-q1jx0n0l	464	5	PH08	PH08	NNP
cord-104268-q1jx0n0l	464	6	gene	gene	NN
cord-104268-q1jx0n0l	464	7	encoding	encode	VBG
cord-104268-q1jx0n0l	464	8	repressible	repressible	JJ
cord-104268-q1jx0n0l	464	9	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	464	10	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	464	11	in	in	IN
cord-104268-q1jx0n0l	464	12	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	464	13	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	465	1	The	the	DT
cord-104268-q1jx0n0l	465	2	molecular	molecular	JJ
cord-104268-q1jx0n0l	465	3	and	and	CC
cord-104268-q1jx0n0l	465	4	cell	cell	NN
cord-104268-q1jx0n0l	465	5	biology	biology	NN
cord-104268-q1jx0n0l	465	6	of	of	IN
cord-104268-q1jx0n0l	465	7	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	466	1	Membrane	membrane	NN
cord-104268-q1jx0n0l	466	2	protein	protein	NN
cord-104268-q1jx0n0l	466	3	sorting	sorting	NN
cord-104268-q1jx0n0l	466	4	:	:	:
cord-104268-q1jx0n0l	466	5	biosynthesis	biosynthesis	NN
cord-104268-q1jx0n0l	466	6	,	,	,
cord-104268-q1jx0n0l	466	7	transport	transport	NN
cord-104268-q1jx0n0l	466	8	and	and	CC
cord-104268-q1jx0n0l	466	9	processing	processing	NN
cord-104268-q1jx0n0l	466	10	of	of	IN
cord-104268-q1jx0n0l	466	11	yeast	yeast	NN
cord-104268-q1jx0n0l	466	12	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	466	13	alkaline	alkaline	JJ
cord-104268-q1jx0n0l	466	14	phosphatase	phosphatase	NN
cord-104268-q1jx0n0l	466	15	.	.	.
cord-104268-q1jx0n0l	467	1	EMBO	EMBO	NNP
cord-104268-q1jx0n0l	468	1	(	(	-LRB-
cord-104268-q1jx0n0l	468	2	Eur	Eur	NNP
cord-104268-q1jx0n0l	468	3	Mot	Mot	NNP
cord-104268-q1jx0n0l	468	4	Epitope	Epitope	NNP
cord-104268-q1jx0n0l	468	5	tagging	tagging	NN
cord-104268-q1jx0n0l	468	6	and	and	CC
cord-104268-q1jx0n0l	468	7	protein	protein	NN
cord-104268-q1jx0n0l	468	8	surveillance	surveillance	NN
cord-104268-q1jx0n0l	468	9	Protein	protein	NN
cord-104268-q1jx0n0l	468	10	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	468	11	in	in	IN
cord-104268-q1jx0n0l	468	12	yeast	yeast	NN
cord-104268-q1jx0n0l	468	13	Rapid	Rapid	NNP
cord-104268-q1jx0n0l	468	14	and	and	CC
cord-104268-q1jx0n0l	468	15	efficient	efficient	JJ
cord-104268-q1jx0n0l	468	16	sitespecific	sitespecific	NN
cord-104268-q1jx0n0l	468	17	mutagenesis	mutagenesis	NN
cord-104268-q1jx0n0l	468	18	without	without	IN
cord-104268-q1jx0n0l	468	19	phenotypic	phenotypic	NN
cord-104268-q1jx0n0l	468	20	selection	selection	NN
cord-104268-q1jx0n0l	468	21	Separation	separation	NN
cord-104268-q1jx0n0l	468	22	and	and	CC
cord-104268-q1jx0n0l	468	23	characterization	characterization	NN
cord-104268-q1jx0n0l	468	24	of	of	IN
cord-104268-q1jx0n0l	468	25	two	two	CD
cord-104268-q1jx0n0l	468	26	al,2-mannosyltransferase	al,2-mannosyltransferase	NNP
cord-104268-q1jx0n0l	468	27	activities	activity	NNS
cord-104268-q1jx0n0l	468	28	from	from	IN
cord-104268-q1jx0n0l	468	29	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	468	30	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	468	31	Protein	protein	NN
cord-104268-q1jx0n0l	468	32	trafficking	traffic	VBG
cord-104268-q1jx0n0l	468	33	in	in	IN
cord-104268-q1jx0n0l	468	34	and	and	CC
cord-104268-q1jx0n0l	468	35	out	out	IN
cord-104268-q1jx0n0l	468	36	of	of	IN
cord-104268-q1jx0n0l	468	37	the	the	DT
cord-104268-q1jx0n0l	468	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	468	39	apparatus	apparatus	NN
cord-104268-q1jx0n0l	468	40	KTR2	KTR2	NNP
cord-104268-q1jx0n0l	468	41	:	:	:
cord-104268-q1jx0n0l	468	42	a	a	DT
cord-104268-q1jx0n0l	468	43	new	new	JJ
cord-104268-q1jx0n0l	468	44	member	member	NN
cord-104268-q1jx0n0l	468	45	of	of	IN
cord-104268-q1jx0n0l	468	46	the	the	DT
cord-104268-q1jx0n0l	468	47	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	468	48	mannosyltransferase	mannosyltransferase	NNP
cord-104268-q1jx0n0l	468	49	gene	gene	NN
cord-104268-q1jx0n0l	468	50	family	family	NN
cord-104268-q1jx0n0l	468	51	Protein	Protein	NNP
cord-104268-q1jx0n0l	468	52	O	o	NN
cord-104268-q1jx0n0l	468	53	-	-	HYPH
cord-104268-q1jx0n0l	468	54	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	468	55	in	in	IN
cord-104268-q1jx0n0l	468	56	yeast	yeast	NN
cord-104268-q1jx0n0l	468	57	.	.	.
cord-104268-q1jx0n0l	469	1	The	the	DT
cord-104268-q1jx0n0l	469	2	PMT2	PMT2	NNP
cord-104268-q1jx0n0l	469	3	gene	gene	NN
cord-104268-q1jx0n0l	469	4	specifies	specify	VBZ
cord-104268-q1jx0n0l	469	5	a	a	DT
cord-104268-q1jx0n0l	469	6	second	second	JJ
cord-104268-q1jx0n0l	469	7	protein	protein	NN
cord-104268-q1jx0n0l	469	8	O	o	NN
cord-104268-q1jx0n0l	469	9	-	-	HYPH
cord-104268-q1jx0n0l	469	10	mannosyltransferase	mannosyltransferase	NNP
cord-104268-q1jx0n0l	469	11	that	that	IN
cord-104268-q1jx0n0l	469	12	functions	function	NNS
cord-104268-q1jx0n0l	469	13	in	in	IN
cord-104268-q1jx0n0l	469	14	addition	addition	NN
cord-104268-q1jx0n0l	469	15	to	to	IN
cord-104268-q1jx0n0l	469	16	the	the	DT
cord-104268-q1jx0n0l	469	17	PMTl	PMTl	NNS
cord-104268-q1jx0n0l	469	18	-	-	HYPH
cord-104268-q1jx0n0l	469	19	encoded	encode	VBN
cord-104268-q1jx0n0l	469	20	activity	activity	NN
cord-104268-q1jx0n0l	469	21	Functional	functional	JJ
cord-104268-q1jx0n0l	469	22	characterization	characterization	NN
cord-104268-q1jx0n0l	469	23	of	of	IN
cord-104268-q1jx0n0l	469	24	the	the	DT
cord-104268-q1jx0n0l	469	25	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	469	26	/	/	SYM
cord-104268-q1jx0n0l	469	27	MNT1	MNT1	NNP
cord-104268-q1jx0n0l	469	28	mannosyltransferase	mannosyltransferase	NN
cord-104268-q1jx0n0l	469	29	gene	gene	NN
cord-104268-q1jx0n0l	469	30	family	family	NN
cord-104268-q1jx0n0l	469	31	Nucleotide	nucleotide	JJ
cord-104268-q1jx0n0l	469	32	sequence	sequence	NN
cord-104268-q1jx0n0l	469	33	analysis	analysis	NN
cord-104268-q1jx0n0l	469	34	of	of	IN
cord-104268-q1jx0n0l	469	35	an	an	DT
cord-104268-q1jx0n0l	469	36	11.7kB	11.7kb	CD
cord-104268-q1jx0n0l	469	37	fragment	fragment	NN
cord-104268-q1jx0n0l	469	38	of	of	IN
cord-104268-q1jx0n0l	469	39	yeast	yeast	NN
cord-104268-q1jx0n0l	469	40	chromosome	chromosome	NN
cord-104268-q1jx0n0l	469	41	I1	i1	NN
cord-104268-q1jx0n0l	469	42	including	include	VBG
cord-104268-q1jx0n0l	469	43	BEM1	BEM1	NNP
cord-104268-q1jx0n0l	469	44	,	,	,
cord-104268-q1jx0n0l	469	45	a	a	DT
cord-104268-q1jx0n0l	469	46	new	new	JJ
cord-104268-q1jx0n0l	469	47	gene	gene	NN
cord-104268-q1jx0n0l	469	48	of	of	IN
cord-104268-q1jx0n0l	469	49	the	the	DT
cord-104268-q1jx0n0l	469	50	WD-40	WD-40	NNP
cord-104268-q1jx0n0l	469	51	repeat	repeat	VB
cord-104268-q1jx0n0l	469	52	family	family	NN
cord-104268-q1jx0n0l	469	53	and	and	CC
cord-104268-q1jx0n0l	469	54	a	a	DT
cord-104268-q1jx0n0l	469	55	new	new	JJ
cord-104268-q1jx0n0l	469	56	member	member	NN
cord-104268-q1jx0n0l	469	57	of	of	IN
cord-104268-q1jx0n0l	469	58	the	the	DT
cord-104268-q1jx0n0l	469	59	KRE2	KRE2	NNP
cord-104268-q1jx0n0l	469	60	/	/	SYM
cord-104268-q1jx0n0l	469	61	MNT1	MNT1	NNP
cord-104268-q1jx0n0l	469	62	family	family	NN
cord-104268-q1jx0n0l	469	63	Sequences	Sequences	NNPS
cord-104268-q1jx0n0l	469	64	within	within	IN
cord-104268-q1jx0n0l	469	65	and	and	CC
cord-104268-q1jx0n0l	469	66	adjacent	adjacent	JJ
cord-104268-q1jx0n0l	469	67	to	to	IN
cord-104268-q1jx0n0l	469	68	the	the	DT
cord-104268-q1jx0n0l	469	69	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	469	70	segment	segment	NN
cord-104268-q1jx0n0l	469	71	of	of	IN
cord-104268-q1jx0n0l	469	72	a-2,6-sialyltransferase	a-2,6-sialyltransferase	NNP
cord-104268-q1jx0n0l	469	73	specify	specify	VB
cord-104268-q1jx0n0l	469	74	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	469	75	retention	retention	NN
cord-104268-q1jx0n0l	469	76	OCHI	OCHI	NNP
cord-104268-q1jx0n0l	469	77	encodes	encode	VBZ
cord-104268-q1jx0n0l	469	78	a	a	DT
cord-104268-q1jx0n0l	469	79	novel	novel	JJ
cord-104268-q1jx0n0l	469	80	membrane	membrane	NN
cord-104268-q1jx0n0l	469	81	bound	bind	VBN
cord-104268-q1jx0n0l	469	82	mannosyltransferase	mannosyltransferase	NNP
cord-104268-q1jx0n0l	469	83	:	:	:
cord-104268-q1jx0n0l	469	84	outer	outer	JJ
cord-104268-q1jx0n0l	469	85	chain	chain	NN
cord-104268-q1jx0n0l	469	86	elongation	elongation	NN
cord-104268-q1jx0n0l	469	87	of	of	IN
cord-104268-q1jx0n0l	469	88	asparagine	asparagine	NN
cord-104268-q1jx0n0l	469	89	-	-	HYPH
cord-104268-q1jx0n0l	469	90	linked	link	VBN
cord-104268-q1jx0n0l	469	91	oligosaccharides	oligosaccharide	NNS
cord-104268-q1jx0n0l	469	92	.	.	.
cord-104268-q1jx0n0l	470	1	EMBO	EMBO	NNP
cord-104268-q1jx0n0l	471	1	(	(	-LRB-
cord-104268-q1jx0n0l	471	2	Eur	Eur	NNP
cord-104268-q1jx0n0l	471	3	Mot	Mot	NNP
cord-104268-q1jx0n0l	472	1	The	the	DT
cord-104268-q1jx0n0l	472	2	membrane	membrane	NN
cord-104268-q1jx0n0l	472	3	spanning	span	VBG
cord-104268-q1jx0n0l	472	4	domain	domain	NN
cord-104268-q1jx0n0l	472	5	of	of	IN
cord-104268-q1jx0n0l	472	6	13	13	CD
cord-104268-q1jx0n0l	472	7	-	-	SYM
cord-104268-q1jx0n0l	472	8	1,4-galactosyltransferase	1,4-galactosyltransferase	CD
cord-104268-q1jx0n0l	473	1	specifies	specify	VBZ
cord-104268-q1jx0n0l	473	2	trans	trans	NNP
cord-104268-q1jx0n0l	473	3	-	-	JJ
cord-104268-q1jx0n0l	473	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	473	5	localization	localization	NN
cord-104268-q1jx0n0l	473	6	Overlapping	overlap	VBG
cord-104268-q1jx0n0l	473	7	distribution	distribution	NN
cord-104268-q1jx0n0l	473	8	of	of	IN
cord-104268-q1jx0n0l	473	9	two	two	CD
cord-104268-q1jx0n0l	473	10	glycosyltransferases	glycosyltransferase	NNS
cord-104268-q1jx0n0l	473	11	in	in	IN
cord-104268-q1jx0n0l	473	12	the	the	DT
cord-104268-q1jx0n0l	473	13	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	473	14	apparatus	apparatus	NN
cord-104268-q1jx0n0l	473	15	of	of	IN
cord-104268-q1jx0n0l	473	16	HeLa	HeLa	NNP
cord-104268-q1jx0n0l	473	17	cells	cell	NNS
cord-104268-q1jx0n0l	473	18	Kin	Kin	NNP
cord-104268-q1jx0n0l	473	19	recognition	recognition	NN
cord-104268-q1jx0n0l	473	20	between	between	IN
cord-104268-q1jx0n0l	473	21	medial	medial	JJ
cord-104268-q1jx0n0l	473	22	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	473	23	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	473	24	in	in	IN
cord-104268-q1jx0n0l	473	25	HeLa	HeLa	NNP
cord-104268-q1jx0n0l	473	26	cells	cell	NNS
cord-104268-q1jx0n0l	473	27	Membrane	membrane	NN
cord-104268-q1jx0n0l	473	28	protein	protein	NN
cord-104268-q1jx0n0l	473	29	retention	retention	NN
cord-104268-q1jx0n0l	473	30	in	in	IN
cord-104268-q1jx0n0l	473	31	the	the	DT
cord-104268-q1jx0n0l	473	32	yeast	yeast	NN
cord-104268-q1jx0n0l	473	33	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	473	34	apparatus	apparatus	NN
cord-104268-q1jx0n0l	473	35	:	:	:
cord-104268-q1jx0n0l	474	1	dipeptidyl	dipeptidyl	NNP
cord-104268-q1jx0n0l	474	2	aminopeptidase	aminopeptidase	NNP
cord-104268-q1jx0n0l	474	3	A	A	NNP
cord-104268-q1jx0n0l	474	4	is	be	VBZ
cord-104268-q1jx0n0l	474	5	retained	retain	VBN
cord-104268-q1jx0n0l	474	6	by	by	IN
cord-104268-q1jx0n0l	474	7	a	a	DT
cord-104268-q1jx0n0l	474	8	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	474	9	signal	signal	NN
cord-104268-q1jx0n0l	474	10	containing	contain	VBG
cord-104268-q1jx0n0l	474	11	aromatic	aromatic	JJ
cord-104268-q1jx0n0l	474	12	residues	residue	NNS
cord-104268-q1jx0n0l	474	13	Sorting	sort	VBG
cord-104268-q1jx0n0l	474	14	of	of	IN
cord-104268-q1jx0n0l	474	15	membrane	membrane	NN
cord-104268-q1jx0n0l	474	16	proteins	protein	NNS
cord-104268-q1jx0n0l	474	17	in	in	IN
cord-104268-q1jx0n0l	474	18	the	the	DT
cord-104268-q1jx0n0l	474	19	secretory	secretory	JJ
cord-104268-q1jx0n0l	474	20	pathway	pathway	NN
cord-104268-q1jx0n0l	475	1	The	the	DT
cord-104268-q1jx0n0l	475	2	TGN38	TGN38	NNP
cord-104268-q1jx0n0l	475	3	glycoprotein	glycoprotein	NN
cord-104268-q1jx0n0l	475	4	contains	contain	VBZ
cord-104268-q1jx0n0l	475	5	two	two	CD
cord-104268-q1jx0n0l	475	6	nonoverlapping	nonoverlappe	VBG
cord-104268-q1jx0n0l	475	7	signals	signal	NNS
cord-104268-q1jx0n0l	475	8	that	that	WDT
cord-104268-q1jx0n0l	475	9	mediate	mediate	VBP
cord-104268-q1jx0n0l	475	10	localization	localization	NN
cord-104268-q1jx0n0l	475	11	to	to	IN
cord-104268-q1jx0n0l	475	12	the	the	DT
cord-104268-q1jx0n0l	475	13	trans	trans	NNP
cord-104268-q1jx0n0l	475	14	-	-	NNP
cord-104268-q1jx0n0l	475	15	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	475	16	network	network	NN
cord-104268-q1jx0n0l	475	17	Characterization	characterization	NN
cord-104268-q1jx0n0l	475	18	of	of	IN
cord-104268-q1jx0n0l	475	19	the	the	DT
cord-104268-q1jx0n0l	475	20	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	475	21	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	475	22	complex	complex	JJ
cord-104268-q1jx0n0l	475	23	through	through	IN
cord-104268-q1jx0n0l	475	24	the	the	DT
cord-104268-q1jx0n0l	475	25	cell	cell	NN
cord-104268-q1jx0n0l	475	26	cycle	cycle	NN
cord-104268-q1jx0n0l	475	27	by	by	IN
cord-104268-q1jx0n0l	475	28	immunoelectron	immunoelectron	NNP
cord-104268-q1jx0n0l	475	29	microscopy	microscopy	NN
cord-104268-q1jx0n0l	475	30	Vesicle	Vesicle	NNP
cord-104268-q1jx0n0l	475	31	-	-	HYPH
cord-104268-q1jx0n0l	475	32	mediated	mediate	VBN
cord-104268-q1jx0n0l	475	33	protein	protein	NN
cord-104268-q1jx0n0l	475	34	sorting	sort	VBG
cord-104268-q1jx0n0l	475	35	Molecular	molecular	JJ
cord-104268-q1jx0n0l	475	36	analysis	analysis	NN
cord-104268-q1jx0n0l	475	37	of	of	IN
cord-104268-q1jx0n0l	475	38	the	the	DT
cord-104268-q1jx0n0l	475	39	yeast	yeast	NN
cord-104268-q1jx0n0l	475	40	VPS3	VPS3	NNP
cord-104268-q1jx0n0l	475	41	gene	gene	NN
cord-104268-q1jx0n0l	475	42	and	and	CC
cord-104268-q1jx0n0l	475	43	the	the	DT
cord-104268-q1jx0n0l	475	44	role	role	NN
cord-104268-q1jx0n0l	475	45	of	of	IN
cord-104268-q1jx0n0l	475	46	its	-PRON-	PRP$
cord-104268-q1jx0n0l	475	47	product	product	NN
cord-104268-q1jx0n0l	475	48	in	in	IN
cord-104268-q1jx0n0l	475	49	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	475	50	protein	protein	NN
cord-104268-q1jx0n0l	475	51	sorting	sorting	NN
cord-104268-q1jx0n0l	475	52	and	and	CC
cord-104268-q1jx0n0l	475	53	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	475	54	segregation	segregation	NN
cord-104268-q1jx0n0l	475	55	during	during	IN
cord-104268-q1jx0n0l	475	56	the	the	DT
cord-104268-q1jx0n0l	475	57	cell	cell	NN
cord-104268-q1jx0n0l	475	58	cycle	cycle	NN
cord-104268-q1jx0n0l	475	59	Immunolocalization	Immunolocalization	NNP
cord-104268-q1jx0n0l	475	60	of	of	IN
cord-104268-q1jx0n0l	475	61	Kex2	Kex2	NNP
cord-104268-q1jx0n0l	475	62	protease	protease	NN
cord-104268-q1jx0n0l	475	63	identifies	identify	VBZ
cord-104268-q1jx0n0l	475	64	a	a	DT
cord-104268-q1jx0n0l	475	65	putative	putative	JJ
cord-104268-q1jx0n0l	475	66	late	late	JJ
cord-104268-q1jx0n0l	475	67	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	475	68	compartment	compartment	NN
cord-104268-q1jx0n0l	475	69	in	in	IN
cord-104268-q1jx0n0l	475	70	the	the	DT
cord-104268-q1jx0n0l	475	71	yeast	yeast	NN
cord-104268-q1jx0n0l	475	72	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	475	73	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	475	74	Structure	structure	NN
cord-104268-q1jx0n0l	475	75	,	,	,
cord-104268-q1jx0n0l	475	76	biosynthesis	biosynthesis	NN
cord-104268-q1jx0n0l	475	77	,	,	,
cord-104268-q1jx0n0l	475	78	and	and	CC
cord-104268-q1jx0n0l	475	79	localization	localization	NN
cord-104268-q1jx0n0l	475	80	of	of	IN
cord-104268-q1jx0n0l	475	81	dipeptidyl	dipeptidyl	JJ
cord-104268-q1jx0n0l	475	82	aminopeptidase	aminopeptidase	NN
cord-104268-q1jx0n0l	475	83	B	B	NNP
cord-104268-q1jx0n0l	475	84	,	,	,
cord-104268-q1jx0n0l	475	85	an	an	DT
cord-104268-q1jx0n0l	475	86	integral	integral	JJ
cord-104268-q1jx0n0l	475	87	membrane	membrane	NN
cord-104268-q1jx0n0l	475	88	glycoprotein	glycoprotein	NN
cord-104268-q1jx0n0l	475	89	of	of	IN
cord-104268-q1jx0n0l	475	90	the	the	DT
cord-104268-q1jx0n0l	475	91	yeast	yeast	NN
cord-104268-q1jx0n0l	475	92	vacuole	vacuole	NN
cord-104268-q1jx0n0l	475	93	Membrane	membrane	NN
cord-104268-q1jx0n0l	475	94	protein	protein	NN
cord-104268-q1jx0n0l	475	95	sorting	sort	VBG
cord-104268-q1jx0n0l	475	96	in	in	IN
cord-104268-q1jx0n0l	475	97	the	the	DT
cord-104268-q1jx0n0l	475	98	yeast	yeast	NN
cord-104268-q1jx0n0l	475	99	secretory	secretory	NN
cord-104268-q1jx0n0l	475	100	pathway	pathway	NN
cord-104268-q1jx0n0l	475	101	:	:	:
cord-104268-q1jx0n0l	475	102	evidence	evidence	NN
cord-104268-q1jx0n0l	475	103	that	that	IN
cord-104268-q1jx0n0l	475	104	the	the	DT
cord-104268-q1jx0n0l	475	105	vacuole	vacuole	NN
cord-104268-q1jx0n0l	475	106	may	may	MD
cord-104268-q1jx0n0l	475	107	be	be	VB
cord-104268-q1jx0n0l	475	108	the	the	DT
cord-104268-q1jx0n0l	475	109	default	default	NN
cord-104268-q1jx0n0l	475	110	compartment	compartment	NN
cord-104268-q1jx0n0l	475	111	Targeting	targeting	NN
cord-104268-q1jx0n0l	475	112	,	,	,
cord-104268-q1jx0n0l	475	113	disruption	disruption	NN
cord-104268-q1jx0n0l	475	114	,	,	,
cord-104268-q1jx0n0l	475	115	replacement	replacement	NN
cord-104268-q1jx0n0l	475	116	,	,	,
cord-104268-q1jx0n0l	475	117	and	and	CC
cord-104268-q1jx0n0l	475	118	allele	allele	NN
cord-104268-q1jx0n0l	476	1	rescue	rescue	NN
cord-104268-q1jx0n0l	476	2	:	:	:
cord-104268-q1jx0n0l	476	3	integrative	integrative	JJ
cord-104268-q1jx0n0l	476	4	DNA	dna	NN
cord-104268-q1jx0n0l	476	5	transformation	transformation	NN
cord-104268-q1jx0n0l	476	6	in	in	IN
cord-104268-q1jx0n0l	476	7	yeast	yeast	NN
cord-104268-q1jx0n0l	476	8	Molecular	Molecular	NNP
cord-104268-q1jx0n0l	476	9	Cloning	Cloning	NNP
cord-104268-q1jx0n0l	476	10	:	:	:
cord-104268-q1jx0n0l	476	11	A	a	DT
cord-104268-q1jx0n0l	476	12	Laboratory	Laboratory	NNP
cord-104268-q1jx0n0l	476	13	Manual	Manual	NNP
cord-104268-q1jx0n0l	476	14	DNA	dna	NN
cord-104268-q1jx0n0l	476	15	sequencing	sequencing	NN
cord-104268-q1jx0n0l	476	16	with	with	IN
cord-104268-q1jx0n0l	476	17	chainterminating	chainterminate	VBG
cord-104268-q1jx0n0l	476	18	inhibitors	inhibitor	NNS
cord-104268-q1jx0n0l	476	19	Characterization	characterization	NN
cord-104268-q1jx0n0l	476	20	of	of	IN
cord-104268-q1jx0n0l	476	21	a	a	DT
cord-104268-q1jx0n0l	476	22	novel	novel	JJ
cord-104268-q1jx0n0l	476	23	63	63	CD
cord-104268-q1jx0n0l	476	24	kDa	kDa	NNS
cord-104268-q1jx0n0l	476	25	membrane	membrane	NN
cord-104268-q1jx0n0l	476	26	protein	protein	NN
cord-104268-q1jx0n0l	476	27	.	.	.
cord-104268-q1jx0n0l	477	1	Implications	implication	NNS
cord-104268-q1jx0n0l	477	2	for	for	IN
cord-104268-q1jx0n0l	477	3	the	the	DT
cord-104268-q1jx0n0l	477	4	organization	organization	NN
cord-104268-q1jx0n0l	477	5	of	of	IN
cord-104268-q1jx0n0l	477	6	the	the	DT
cord-104268-q1jx0n0l	477	7	ER	ER	NNP
cord-104268-q1jx0n0l	477	8	-	-	HYPH
cord-104268-q1jx0n0l	477	9	to	to	IN
cord-104268-q1jx0n0l	477	10	-	-	HYPH
cord-104268-q1jx0n0l	477	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	477	12	pathway	pathway	NN
cord-104268-q1jx0n0l	477	13	Retention	Retention	NNP
cord-104268-q1jx0n0l	477	14	of	of	IN
cord-104268-q1jx0n0l	477	15	p63	p63	CD
cord-104268-q1jx0n0l	477	16	in	in	IN
cord-104268-q1jx0n0l	477	17	an	an	DT
cord-104268-q1jx0n0l	477	18	ER	ER	NNP
cord-104268-q1jx0n0l	477	19	-	-	HYPH
cord-104268-q1jx0n0l	477	20	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	477	21	intermediate	intermediate	JJ
cord-104268-q1jx0n0l	477	22	compartment	compartment	NN
cord-104268-q1jx0n0l	477	23	depends	depend	VBZ
cord-104268-q1jx0n0l	477	24	on	on	IN
cord-104268-q1jx0n0l	477	25	the	the	DT
cord-104268-q1jx0n0l	477	26	presence	presence	NN
cord-104268-q1jx0n0l	477	27	of	of	IN
cord-104268-q1jx0n0l	477	28	all	all	DT
cord-104268-q1jx0n0l	477	29	three	three	CD
cord-104268-q1jx0n0l	477	30	of	of	IN
cord-104268-q1jx0n0l	477	31	its	-PRON-	PRP$
cord-104268-q1jx0n0l	477	32	domains	domain	NNS
cord-104268-q1jx0n0l	477	33	and	and	CC
cord-104268-q1jx0n0l	477	34	on	on	IN
cord-104268-q1jx0n0l	477	35	its	-PRON-	PRP$
cord-104268-q1jx0n0l	477	36	ability	ability	NN
cord-104268-q1jx0n0l	477	37	to	to	TO
cord-104268-q1jx0n0l	477	38	form	form	VB
cord-104268-q1jx0n0l	477	39	oligomers	oligomer	NNS
cord-104268-q1jx0n0l	477	40	Selective	selective	JJ
cord-104268-q1jx0n0l	477	41	and	and	CC
cord-104268-q1jx0n0l	477	42	immediate	immediate	JJ
cord-104268-q1jx0n0l	477	43	effects	effect	NNS
cord-104268-q1jx0n0l	477	44	of	of	IN
cord-104268-q1jx0n0l	477	45	clathrin	clathrin	NN
cord-104268-q1jx0n0l	477	46	heavy	heavy	JJ
cord-104268-q1jx0n0l	477	47	chain	chain	NN
cord-104268-q1jx0n0l	477	48	mutations	mutation	NNS
cord-104268-q1jx0n0l	477	49	on	on	IN
cord-104268-q1jx0n0l	477	50	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	477	51	membrane	membrane	NN
cord-104268-q1jx0n0l	477	52	protein	protein	NN
cord-104268-q1jx0n0l	477	53	retention	retention	NN
cord-104268-q1jx0n0l	477	54	in	in	IN
cord-104268-q1jx0n0l	477	55	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	477	56	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	477	57	Enzymes	enzyme	NNS
cord-104268-q1jx0n0l	477	58	associated	associate	VBN
cord-104268-q1jx0n0l	477	59	with	with	IN
cord-104268-q1jx0n0l	477	60	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	477	61	A	a	DT
cord-104268-q1jx0n0l	477	62	system	system	NN
cord-104268-q1jx0n0l	477	63	of	of	IN
cord-104268-q1jx0n0l	477	64	shuttle	shuttle	NN
cord-104268-q1jx0n0l	477	65	vectors	vector	NNS
cord-104268-q1jx0n0l	477	66	and	and	CC
cord-104268-q1jx0n0l	477	67	yeast	yeast	NN
cord-104268-q1jx0n0l	477	68	host	host	NN
cord-104268-q1jx0n0l	477	69	strains	strain	NNS
cord-104268-q1jx0n0l	477	70	designed	design	VBN
cord-104268-q1jx0n0l	477	71	for	for	IN
cord-104268-q1jx0n0l	477	72	efficient	efficient	JJ
cord-104268-q1jx0n0l	477	73	manipulation	manipulation	NN
cord-104268-q1jx0n0l	477	74	of	of	IN
cord-104268-q1jx0n0l	477	75	DNA	dna	NN
cord-104268-q1jx0n0l	477	76	in	in	IN
cord-104268-q1jx0n0l	477	77	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	477	78	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	477	79	Isolation	isolation	NN
cord-104268-q1jx0n0l	477	80	of	of	IN
cord-104268-q1jx0n0l	477	81	a	a	DT
cord-104268-q1jx0n0l	477	82	matrix	matrix	NN
cord-104268-q1jx0n0l	477	83	that	that	WDT
cord-104268-q1jx0n0l	477	84	binds	bind	VBZ
cord-104268-q1jx0n0l	477	85	medial	medial	JJ
cord-104268-q1jx0n0l	477	86	-	-	HYPH
cord-104268-q1jx0n0l	477	87	Golgi	golgi	NN
cord-104268-q1jx0n0l	477	88	enzymes	enzyme	NNS
cord-104268-q1jx0n0l	477	89	A	a	DT
cord-104268-q1jx0n0l	477	90	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	477	91	retention	retention	NN
cord-104268-q1jx0n0l	477	92	signal	signal	NN
cord-104268-q1jx0n0l	477	93	in	in	IN
cord-104268-q1jx0n0l	477	94	a	a	DT
cord-104268-q1jx0n0l	477	95	membrane	membrane	NN
cord-104268-q1jx0n0l	477	96	-	-	HYPH
cord-104268-q1jx0n0l	477	97	spanning	span	VBG
cord-104268-q1jx0n0l	477	98	domain	domain	NN
cord-104268-q1jx0n0l	477	99	of	of	IN
cord-104268-q1jx0n0l	477	100	coronavirus	coronavirus	NN
cord-104268-q1jx0n0l	477	101	E1	e1	NN
cord-104268-q1jx0n0l	477	102	protein	protein	NN
cord-104268-q1jx0n0l	478	1	The	the	DT
cord-104268-q1jx0n0l	478	2	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	478	3	domain	domain	NN
cord-104268-q1jx0n0l	478	4	of	of	IN
cord-104268-q1jx0n0l	478	5	N	n	NN
cord-104268-q1jx0n0l	478	6	-	-	HYPH
cord-104268-q1jx0n0l	478	7	glucosaminyltransferase	glucosaminyltransferase	NN
cord-104268-q1jx0n0l	479	1	I	-PRON-	PRP
cord-104268-q1jx0n0l	479	2	contains	contain	VBZ
cord-104268-q1jx0n0l	479	3	a	a	DT
cord-104268-q1jx0n0l	479	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	479	5	retention	retention	NN
cord-104268-q1jx0n0l	479	6	signal	signal	NN
cord-104268-q1jx0n0l	480	1	The	the	DT
cord-104268-q1jx0n0l	480	2	signal	signal	NN
cord-104268-q1jx0n0l	480	3	for	for	IN
cord-104268-q1jx0n0l	480	4	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	480	5	retention	retention	NN
cord-104268-q1jx0n0l	480	6	of	of	IN
cord-104268-q1jx0n0l	480	7	bovine	bovine	JJ
cord-104268-q1jx0n0l	480	8	131,4-galactosyltransferase	131,4-galactosyltransferase	CD
cord-104268-q1jx0n0l	480	9	is	be	VBZ
cord-104268-q1jx0n0l	480	10	the	the	DT
cord-104268-q1jx0n0l	480	11	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	480	12	domain	domain	NN
cord-104268-q1jx0n0l	480	13	Oligomerization	Oligomerization	NNP
cord-104268-q1jx0n0l	480	14	of	of	IN
cord-104268-q1jx0n0l	480	15	a	a	DT
cord-104268-q1jx0n0l	480	16	membrane	membrane	NN
cord-104268-q1jx0n0l	480	17	protein	protein	NN
cord-104268-q1jx0n0l	480	18	correlates	correlate	VBZ
cord-104268-q1jx0n0l	480	19	with	with	IN
cord-104268-q1jx0n0l	480	20	its	-PRON-	PRP$
cord-104268-q1jx0n0l	480	21	retention	retention	NN
cord-104268-q1jx0n0l	480	22	in	in	IN
cord-104268-q1jx0n0l	480	23	the	the	DT
cord-104268-q1jx0n0l	480	24	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	480	25	complex	complex	JJ
cord-104268-q1jx0n0l	480	26	Mutation	mutation	NN
cord-104268-q1jx0n0l	480	27	of	of	IN
cord-104268-q1jx0n0l	480	28	a	a	DT
cord-104268-q1jx0n0l	480	29	tyrosine	tyrosine	NN
cord-104268-q1jx0n0l	480	30	localization	localization	NN
cord-104268-q1jx0n0l	480	31	signal	signal	NN
cord-104268-q1jx0n0l	480	32	in	in	IN
cord-104268-q1jx0n0l	480	33	the	the	DT
cord-104268-q1jx0n0l	480	34	cytosolic	cytosolic	JJ
cord-104268-q1jx0n0l	480	35	tail	tail	NN
cord-104268-q1jx0n0l	480	36	of	of	IN
cord-104268-q1jx0n0l	480	37	Kex2p	Kex2p	NNP
cord-104268-q1jx0n0l	480	38	protease	protease	NN
cord-104268-q1jx0n0l	480	39	disrupts	disrupt	VBZ
cord-104268-q1jx0n0l	480	40	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	480	41	retention	retention	NN
cord-104268-q1jx0n0l	480	42	and	and	CC
cord-104268-q1jx0n0l	480	43	results	result	NNS
cord-104268-q1jx0n0l	480	44	in	in	IN
cord-104268-q1jx0n0l	480	45	default	default	NN
cord-104268-q1jx0n0l	480	46	transport	transport	NN
cord-104268-q1jx0n0l	480	47	to	to	IN
cord-104268-q1jx0n0l	480	48	the	the	DT
cord-104268-q1jx0n0l	480	49	vacuole	vacuole	NN
cord-104268-q1jx0n0l	480	50	Dynamic	dynamic	JJ
cord-104268-q1jx0n0l	480	51	retention	retention	NN
cord-104268-q1jx0n0l	480	52	of	of	IN
cord-104268-q1jx0n0l	480	53	TGN	TGN	NNP
cord-104268-q1jx0n0l	480	54	membrane	membrane	NN
cord-104268-q1jx0n0l	480	55	proteins	protein	NNS
cord-104268-q1jx0n0l	480	56	in	in	IN
cord-104268-q1jx0n0l	480	57	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	480	58	cerevisiae	cerevisiae	NNS
cord-104268-q1jx0n0l	481	1	The	the	DT
cord-104268-q1jx0n0l	481	2	17-residue	17-residue	CD
cord-104268-q1jx0n0l	481	3	transmembrane	transmembrane	NN
cord-104268-q1jx0n0l	481	4	domain	domain	NN
cord-104268-q1jx0n0l	481	5	of	of	IN
cord-104268-q1jx0n0l	481	6	13-galactoside	13-galactoside	CD
cord-104268-q1jx0n0l	481	7	ct2,6-sialyltransferase	ct2,6-sialyltransferase	NNP
cord-104268-q1jx0n0l	481	8	is	be	VBZ
cord-104268-q1jx0n0l	481	9	sufficient	sufficient	JJ
cord-104268-q1jx0n0l	481	10	for	for	IN
cord-104268-q1jx0n0l	481	11	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	481	12	retention	retention	NN
cord-104268-q1jx0n0l	482	1	The	the	DT
cord-104268-q1jx0n0l	482	2	SXYQRL	SXYQRL	NNP
cord-104268-q1jx0n0l	482	3	sequence	sequence	NN
cord-104268-q1jx0n0l	482	4	in	in	IN
cord-104268-q1jx0n0l	482	5	the	the	DT
cord-104268-q1jx0n0l	482	6	cytoplasmic	cytoplasmic	JJ
cord-104268-q1jx0n0l	482	7	domain	domain	NN
cord-104268-q1jx0n0l	482	8	of	of	IN
cord-104268-q1jx0n0l	482	9	TGN38	TGN38	NNP
cord-104268-q1jx0n0l	482	10	plays	play	VBZ
cord-104268-q1jx0n0l	482	11	a	a	DT
cord-104268-q1jx0n0l	482	12	major	major	JJ
cord-104268-q1jx0n0l	482	13	role	role	NN
cord-104268-q1jx0n0l	482	14	in	in	IN
cord-104268-q1jx0n0l	482	15	the	the	DT
cord-104268-q1jx0n0l	482	16	trans	trans	NNP
cord-104268-q1jx0n0l	482	17	-	-	NNP
cord-104268-q1jx0n0l	482	18	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	482	19	network	network	NN
cord-104268-q1jx0n0l	482	20	localization	localization	NN
cord-104268-q1jx0n0l	482	21	The	the	DT
cord-104268-q1jx0n0l	482	22	PEP4	PEP4	NNP
cord-104268-q1jx0n0l	482	23	gene	gene	NN
cord-104268-q1jx0n0l	482	24	encodes	encode	VBZ
cord-104268-q1jx0n0l	482	25	an	an	DT
cord-104268-q1jx0n0l	482	26	aspartyl	aspartyl	NNP
cord-104268-q1jx0n0l	482	27	protease	protease	NN
cord-104268-q1jx0n0l	482	28	implicated	implicate	VBN
cord-104268-q1jx0n0l	482	29	in	in	IN
cord-104268-q1jx0n0l	482	30	the	the	DT
cord-104268-q1jx0n0l	482	31	posttranslational	posttranslational	JJ
cord-104268-q1jx0n0l	482	32	regulation	regulation	NN
cord-104268-q1jx0n0l	482	33	of	of	IN
cord-104268-q1jx0n0l	482	34	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	482	35	cerevisiae	cerevisiae	VBZ
cord-104268-q1jx0n0l	482	36	vacuolar	vacuolar	NNP
cord-104268-q1jx0n0l	482	37	hydrolases	hydrolase	NNS
cord-104268-q1jx0n0l	482	38	Golgi	Golgi	NNP
cord-104268-q1jx0n0l	482	39	retention	retention	NN
cord-104268-q1jx0n0l	482	40	mechanism	mechanism	NN
cord-104268-q1jx0n0l	482	41	of	of	IN
cord-104268-q1jx0n0l	482	42	13	13	CD
cord-104268-q1jx0n0l	482	43	-	-	SYM
cord-104268-q1jx0n0l	482	44	1,4-galactosyltransferase	1,4-galactosyltransferase	CD
cord-104268-q1jx0n0l	482	45	Cloning	cloning	NN
cord-104268-q1jx0n0l	482	46	and	and	CC
cord-104268-q1jx0n0l	482	47	analysis	analysis	NN
cord-104268-q1jx0n0l	482	48	of	of	IN
cord-104268-q1jx0n0l	482	49	the	the	DT
cord-104268-q1jx0n0l	482	50	Saccharomyces	Saccharomyces	NNP
cord-104268-q1jx0n0l	482	51	cerevisine	cerevisine	NN
cord-104268-q1jx0n0l	482	52	MNN9	MNN9	NNP
cord-104268-q1jx0n0l	482	53	and	and	CC
cord-104268-q1jx0n0l	482	54	MNN1	MNN1	NNP
cord-104268-q1jx0n0l	482	55	genes	gene	NNS
cord-104268-q1jx0n0l	482	56	required	require	VBN
cord-104268-q1jx0n0l	482	57	for	for	IN
cord-104268-q1jx0n0l	482	58	complex	complex	JJ
cord-104268-q1jx0n0l	482	59	glycosylation	glycosylation	NN
cord-104268-q1jx0n0l	482	60	of	of	IN
cord-104268-q1jx0n0l	482	61	secreted	secrete	VBN
cord-104268-q1jx0n0l	482	62	proteins	protein	NNS
cord-104268-q1jx0n0l	483	1	We	-PRON-	PRP
cord-104268-q1jx0n0l	483	2	thank	thank	VBP
cord-104268-q1jx0n0l	483	3	Dr.	Dr.	NNP
cord-104268-q1jx0n0l	483	4	Annette	Annette	NNP
cord-104268-q1jx0n0l	483	5	Herscovics	Herscovics	NNP
cord-104268-q1jx0n0l	483	6	for	for	IN
cord-104268-q1jx0n0l	483	7	critically	critically	RB
cord-104268-q1jx0n0l	483	8	reading	read	VBG
cord-104268-q1jx0n0l	483	9	the	the	DT
cord-104268-q1jx0n0l	483	10	manuscript	manuscript	NN
cord-104268-q1jx0n0l	483	11	,	,	,
cord-104268-q1jx0n0l	483	12	the	the	DT
cord-104268-q1jx0n0l	483	13	members	member	NNS
cord-104268-q1jx0n0l	483	14	of	of	IN
cord-104268-q1jx0n0l	483	15	the	the	DT
cord-104268-q1jx0n0l	483	16	Bussey	Bussey	NNP
cord-104268-q1jx0n0l	483	17	laboratory	laboratory	NN
cord-104268-q1jx0n0l	483	18	for	for	IN
cord-104268-q1jx0n0l	483	19	helpful	helpful	JJ
cord-104268-q1jx0n0l	483	20	comments	comment	NNS
cord-104268-q1jx0n0l	483	21	and	and	CC
cord-104268-q1jx0n0l	483	22	suggestions	suggestion	NNS
cord-104268-q1jx0n0l	483	23	,	,	,
cord-104268-q1jx0n0l	483	24	Antony	Antony	NNP
cord-104268-q1jx0n0l	483	25	Cooper	Cooper	NNP
cord-104268-q1jx0n0l	483	26	and	and	CC
cord-104268-q1jx0n0l	483	27	Kathryn	Kathryn	NNP
cord-104268-q1jx0n0l	483	28	Hill	Hill	NNP
cord-104268-q1jx0n0l	483	29	for	for	IN
cord-104268-q1jx0n0l	483	30	all	all	PDT
cord-104268-q1jx0n0l	483	31	their	-PRON-	PRP$
cord-104268-q1jx0n0l	483	32	help	help	NN
cord-104268-q1jx0n0l	483	33	and	and	CC
cord-104268-q1jx0n0l	483	34	advice	advice	NN
cord-104268-q1jx0n0l	483	35	,	,	,
cord-104268-q1jx0n0l	483	36	Tom	Tom	NNP
cord-104268-q1jx0n0l	483	37	Stevens	Stevens	NNP
cord-104268-q1jx0n0l	483	38	for	for	IN
cord-104268-q1jx0n0l	483	39	kindly	kindly	RB
cord-104268-q1jx0n0l	483	40	providing	provide	VBG
cord-104268-q1jx0n0l	483	41	strains	strain	NNS
cord-104268-q1jx0n0l	483	42	,	,	,
cord-104268-q1jx0n0l	483	43	plasmids	plasmid	NNS
cord-104268-q1jx0n0l	483	44	,	,	,
cord-104268-q1jx0n0l	483	45	and	and	CC
cord-104268-q1jx0n0l	483	46	antibodies	antibody	NNS
cord-104268-q1jx0n0l	483	47	,	,	,
cord-104268-q1jx0n0l	483	48	Anne	Anne	NNP
cord-104268-q1jx0n0l	483	49	Camirand	Camirand	NNP
cord-104268-q1jx0n0l	483	50	for	for	IN
cord-104268-q1jx0n0l	483	51	advice	advice	NN
cord-104268-q1jx0n0l	483	52	with	with	IN
cord-104268-q1jx0n0l	483	53	the	the	DT
cord-104268-q1jx0n0l	483	54	mannosyltransferase	mannosyltransferase	NNP
cord-104268-q1jx0n0l	483	55	assays	assay	NNS
cord-104268-q1jx0n0l	483	56	,	,	,
cord-104268-q1jx0n0l	483	57	Rowan	Rowan	NNP
cord-104268-q1jx0n0l	483	58	Chapman	Chapman	NNP
cord-104268-q1jx0n0l	483	59	for	for	IN
cord-104268-q1jx0n0l	483	60	discussion	discussion	NN
cord-104268-q1jx0n0l	483	61	and	and	CC
cord-104268-q1jx0n0l	483	62	plasmids	plasmid	NNS
cord-104268-q1jx0n0l	483	63	,	,	,
cord-104268-q1jx0n0l	483	64	V.	V.	NNP
cord-104268-q1jx0n0l	483	65	Mackay	Mackay	NNP
cord-104268-q1jx0n0l	483	66	and	and	CC
cord-104268-q1jx0n0l	483	67	C.	C.	NNP
cord-104268-q1jx0n0l	483	68	Yip	Yip	NNP
cord-104268-q1jx0n0l	483	69	for	for	IN
cord-104268-q1jx0n0l	483	70	the	the	DT
cord-104268-q1jx0n0l	483	71	MNN1	MNN1	NNP
cord-104268-q1jx0n0l	483	72	clone	clone	NN
cord-104268-q1jx0n0l	483	73	,	,	,
cord-104268-q1jx0n0l	483	74	Carole	Carole	NNP
cord-104268-q1jx0n0l	483	75	Smith	Smith	NNP
cord-104268-q1jx0n0l	483	76	and	and	CC
cord-104268-q1jx0n0l	483	77	Guy	Guy	NNP
cord-104268-q1jx0n0l	483	78	I'Heureux	I'Heureux	NNP
cord-104268-q1jx0n0l	483	79	for	for	IN
cord-104268-q1jx0n0l	483	80	photographic	photographic	JJ
cord-104268-q1jx0n0l	483	81	work	work	NN
cord-104268-q1jx0n0l	483	82	,	,	,
cord-104268-q1jx0n0l	483	83	and	and	CC
cord-104268-q1jx0n0l	483	84	Diane	Diane	NNP
cord-104268-q1jx0n0l	483	85	Oki	Oki	NNP
cord-104268-q1jx0n0l	483	86	for	for	IN
cord-104268-q1jx0n0l	483	87	assistance	assistance	NN
cord-104268-q1jx0n0l	483	88	with	with	IN
cord-104268-q1jx0n0l	483	89	manuscript	manuscript	NN
cord-104268-q1jx0n0l	483	90	preparation	preparation	NN
cord-104268-q1jx0n0l	483	91	.	.	.
cord-104268-q1jx0n0l	484	1	Supported	support	VBN
cord-104268-q1jx0n0l	484	2	by	by	IN
cord-104268-q1jx0n0l	484	3	an	an	DT
cord-104268-q1jx0n0l	484	4	Operating	Operating	NNP
cord-104268-q1jx0n0l	484	5	Grant	Grant	NNP
cord-104268-q1jx0n0l	484	6	from	from	IN
cord-104268-q1jx0n0l	484	7	the	the	DT
cord-104268-q1jx0n0l	484	8	Natural	Natural	NNP
cord-104268-q1jx0n0l	484	9	Sciences	Sciences	NNPS
cord-104268-q1jx0n0l	484	10	and	and	CC
cord-104268-q1jx0n0l	484	11	Engineering	Engineering	NNP
cord-104268-q1jx0n0l	484	12	Research	Research	NNP
cord-104268-q1jx0n0l	484	13	Council	Council	NNP
cord-104268-q1jx0n0l	484	14	of	of	IN
cord-104268-q1jx0n0l	484	15	Canada	Canada	NNP
cord-104268-q1jx0n0l	484	16	.	.	.
cord-104268-q1jx0n0l	485	1	M.	M.	NNP
cord-104268-q1jx0n0l	485	2	Lussier	Lussier	NNP
cord-104268-q1jx0n0l	485	3	was	be	VBD
cord-104268-q1jx0n0l	485	4	the	the	DT
cord-104268-q1jx0n0l	485	5	recipient	recipient	NN
cord-104268-q1jx0n0l	485	6	of	of	IN
cord-104268-q1jx0n0l	485	7	a	a	DT
cord-104268-q1jx0n0l	485	8	postdoctoral	postdoctoral	JJ
cord-104268-q1jx0n0l	485	9	fellowship	fellowship	NN
cord-104268-q1jx0n0l	485	10	from	from	IN
cord-104268-q1jx0n0l	485	11	the	the	DT
cord-104268-q1jx0n0l	485	12	Medical	Medical	NNP
cord-104268-q1jx0n0l	485	13	Research	Research	NNP
cord-104268-q1jx0n0l	485	14	Council	Council	NNP
cord-104268-q1jx0n0l	485	15	of	of	IN
cord-104268-q1jx0n0l	485	16	Canada	Canada	NNP
cord-104268-q1jx0n0l	485	17	.	.	.
cord-104268-q1jx0n0l	486	1	Received	receive	VBN
cord-104268-q1jx0n0l	486	2	for	for	IN
cord-104268-q1jx0n0l	486	3	publication	publication	NN
cord-104268-q1jx0n0l	486	4	12	12	CD
cord-104268-q1jx0n0l	486	5	May	May	NNP
cord-104268-q1jx0n0l	486	6	1995	1995	CD
cord-104268-q1jx0n0l	486	7	and	and	CC
cord-104268-q1jx0n0l	486	8	in	in	IN
cord-104268-q1jx0n0l	486	9	revised	revise	VBN
cord-104268-q1jx0n0l	486	10	form	form	NN
cord-104268-q1jx0n0l	486	11	27	27	CD
cord-104268-q1jx0n0l	486	12	July	July	NNP
cord-104268-q1jx0n0l	486	13	1995	1995	CD
cord-104268-q1jx0n0l	486	14	.	.	.