id sid tid token lemma pos cord-008590-xivnsldf 1 1 key key JJ cord-008590-xivnsldf 1 2 : : : cord-008590-xivnsldf 1 3 cord-008590-xivnsldf cord-008590-xivnsldf . cord-008590-xivnsldf 2 1 authors author NNS cord-008590-xivnsldf 2 2 : : : cord-008590-xivnsldf 2 3 Tartakoff Tartakoff NNP cord-008590-xivnsldf 2 4 , , , cord-008590-xivnsldf 2 5 Alan Alan NNP cord-008590-xivnsldf 2 6 M. M. NNP cord-008590-xivnsldf 2 7 title title NN cord-008590-xivnsldf 2 8 : : : cord-008590-xivnsldf 2 9 The the DT cord-008590-xivnsldf 2 10 Confined Confined NNP cord-008590-xivnsldf 2 11 Function Function NNP cord-008590-xivnsldf 2 12 Model Model NNP cord-008590-xivnsldf 2 13 of of IN cord-008590-xivnsldf 2 14 the the DT cord-008590-xivnsldf 2 15 Golgi Golgi NNP cord-008590-xivnsldf 2 16 Complex Complex NNP cord-008590-xivnsldf 2 17 : : : cord-008590-xivnsldf 2 18 Center Center NNP cord-008590-xivnsldf 2 19 for for IN cord-008590-xivnsldf 2 20 Ordered order VBN cord-008590-xivnsldf 2 21 Processing Processing NNP cord-008590-xivnsldf 2 22 of of IN cord-008590-xivnsldf 2 23 Biosynthetic Biosynthetic NNP cord-008590-xivnsldf 2 24 Products Products NNPS cord-008590-xivnsldf 2 25 of of IN cord-008590-xivnsldf 2 26 the the DT cord-008590-xivnsldf 2 27 Rough Rough NNP cord-008590-xivnsldf 2 28 Endoplasmic Endoplasmic NNP cord-008590-xivnsldf 2 29 Reticulum Reticulum NNP cord-008590-xivnsldf 2 30 date date NN cord-008590-xivnsldf 2 31 : : : cord-008590-xivnsldf 2 32 2008 2008 CD cord-008590-xivnsldf 2 33 - - HYPH cord-008590-xivnsldf 2 34 04 04 CD cord-008590-xivnsldf 2 35 - - SYM cord-008590-xivnsldf 2 36 14 14 CD cord-008590-xivnsldf 2 37 journal journal NN cord-008590-xivnsldf 2 38 : : : cord-008590-xivnsldf 3 1 Int Int NNP cord-008590-xivnsldf 3 2 Rev Rev NNP cord-008590-xivnsldf 3 3 Cytol Cytol NNP cord-008590-xivnsldf 3 4 DOI DOI NNP cord-008590-xivnsldf 3 5 : : : cord-008590-xivnsldf 3 6 10.1016 10.1016 CD cord-008590-xivnsldf 3 7 / / SYM cord-008590-xivnsldf 3 8 s0074 s0074 NNP cord-008590-xivnsldf 3 9 - - HYPH cord-008590-xivnsldf 3 10 7696(08)62374 7696(08)62374 NNP cord-008590-xivnsldf 3 11 - - HYPH cord-008590-xivnsldf 3 12 8 8 CD cord-008590-xivnsldf 3 13 sha sha NNP cord-008590-xivnsldf 3 14 : : : cord-008590-xivnsldf 4 1 960b33f5eb0be2824e641528596616989306d470 960b33f5eb0be2824e641528596616989306d470 CD cord-008590-xivnsldf 4 2 doc_id doc_id CD cord-008590-xivnsldf 5 1 : : : cord-008590-xivnsldf 5 2 8590 8590 CD cord-008590-xivnsldf 5 3 cord_uid cord_uid NNS cord-008590-xivnsldf 5 4 : : : cord-008590-xivnsldf 6 1 xivnsldf xivnsldf NNP cord-008590-xivnsldf 7 1 The the DT cord-008590-xivnsldf 7 2 organized organized JJ cord-008590-xivnsldf 7 3 and and CC cord-008590-xivnsldf 7 4 characteristic characteristic JJ cord-008590-xivnsldf 7 5 elements element NNS cord-008590-xivnsldf 7 6 of of IN cord-008590-xivnsldf 7 7 the the DT cord-008590-xivnsldf 7 8 Golgi Golgi NNP cord-008590-xivnsldf 7 9 complex complex NN cord-008590-xivnsldf 7 10 ( ( -LRB- cord-008590-xivnsldf 7 11 GC GC NNP cord-008590-xivnsldf 7 12 ) ) -RRB- cord-008590-xivnsldf 7 13 are be VBP cord-008590-xivnsldf 7 14 the the DT cord-008590-xivnsldf 7 15 stacked stack VBN cord-008590-xivnsldf 7 16 smooth smooth RB cord-008590-xivnsldf 7 17 - - HYPH cord-008590-xivnsldf 7 18 surfaced surface VBN cord-008590-xivnsldf 7 19 cisternae cisternae NNP cord-008590-xivnsldf 7 20 , , , cord-008590-xivnsldf 7 21 which which WDT cord-008590-xivnsldf 7 22 are be VBP cord-008590-xivnsldf 7 23 found find VBN cord-008590-xivnsldf 7 24 in in IN cord-008590-xivnsldf 7 25 the the DT cord-008590-xivnsldf 7 26 centrosphere centrosphere NN cord-008590-xivnsldf 7 27 of of IN cord-008590-xivnsldf 7 28 all all DT cord-008590-xivnsldf 7 29 eukaryotic eukaryotic JJ cord-008590-xivnsldf 7 30 cells cell NNS cord-008590-xivnsldf 7 31 . . . cord-008590-xivnsldf 8 1 These these DT cord-008590-xivnsldf 8 2 cisternae cisternae NNP cord-008590-xivnsldf 8 3 , , , cord-008590-xivnsldf 8 4 in in IN cord-008590-xivnsldf 8 5 conjunction conjunction NN cord-008590-xivnsldf 8 6 with with IN cord-008590-xivnsldf 8 7 other other JJ cord-008590-xivnsldf 8 8 associated associated JJ cord-008590-xivnsldf 8 9 smooth smooth RB cord-008590-xivnsldf 8 10 - - HYPH cord-008590-xivnsldf 8 11 surfaced surface VBN cord-008590-xivnsldf 8 12 membranes membrane NNS cord-008590-xivnsldf 8 13 , , , cord-008590-xivnsldf 8 14 are be VBP cord-008590-xivnsldf 8 15 responsible responsible JJ cord-008590-xivnsldf 8 16 for for IN cord-008590-xivnsldf 8 17 executing execute VBG cord-008590-xivnsldf 8 18 net net JJ cord-008590-xivnsldf 8 19 unidirectional unidirectional JJ cord-008590-xivnsldf 8 20 intracellular intracellular JJ cord-008590-xivnsldf 8 21 transport transport NN cord-008590-xivnsldf 8 22 ( ( -LRB- cord-008590-xivnsldf 8 23 ICT ICT NNP cord-008590-xivnsldf 8 24 ) ) -RRB- cord-008590-xivnsldf 8 25 from from IN cord-008590-xivnsldf 8 26 the the DT cord-008590-xivnsldf 8 27 rough rough JJ cord-008590-xivnsldf 8 28 endoplasmic endoplasmic NN cord-008590-xivnsldf 8 29 reticulum reticulum NN cord-008590-xivnsldf 8 30 ( ( -LRB- cord-008590-xivnsldf 8 31 RER RER NNP cord-008590-xivnsldf 8 32 ) ) -RRB- cord-008590-xivnsldf 8 33 toward toward IN cord-008590-xivnsldf 8 34 more more RBR cord-008590-xivnsldf 8 35 distally distally RB cord-008590-xivnsldf 8 36 located locate VBN cord-008590-xivnsldf 8 37 structures structure NNS cord-008590-xivnsldf 8 38 . . . cord-008590-xivnsldf 9 1 This this DT cord-008590-xivnsldf 9 2 chapter chapter NN cord-008590-xivnsldf 9 3 focuses focus VBZ cord-008590-xivnsldf 9 4 on on IN cord-008590-xivnsldf 9 5 the the DT cord-008590-xivnsldf 9 6 broad broad JJ cord-008590-xivnsldf 9 7 range range NN cord-008590-xivnsldf 9 8 of of IN cord-008590-xivnsldf 9 9 accessory accessory JJ cord-008590-xivnsldf 9 10 activities activity NNS cord-008590-xivnsldf 9 11 that that WDT cord-008590-xivnsldf 9 12 occur occur VBP cord-008590-xivnsldf 9 13 during during IN cord-008590-xivnsldf 9 14 transport transport NN cord-008590-xivnsldf 9 15 , , , cord-008590-xivnsldf 9 16 the the DT cord-008590-xivnsldf 9 17 family family NN cord-008590-xivnsldf 9 18 of of IN cord-008590-xivnsldf 9 19 “ " `` cord-008590-xivnsldf 9 20 posttranslational posttranslational JJ cord-008590-xivnsldf 9 21 modifications modification NNS cord-008590-xivnsldf 9 22 . . . cord-008590-xivnsldf 9 23 ” " '' cord-008590-xivnsldf 10 1 These these DT cord-008590-xivnsldf 10 2 events event NNS cord-008590-xivnsldf 10 3 are be VBP cord-008590-xivnsldf 10 4 , , , cord-008590-xivnsldf 10 5 in in IN cord-008590-xivnsldf 10 6 all all DT cord-008590-xivnsldf 10 7 likelihood likelihood NN cord-008590-xivnsldf 10 8 , , , cord-008590-xivnsldf 10 9 not not RB cord-008590-xivnsldf 10 10 essential essential JJ cord-008590-xivnsldf 10 11 for for IN cord-008590-xivnsldf 10 12 the the DT cord-008590-xivnsldf 10 13 “ " `` cord-008590-xivnsldf 10 14 primary primary JJ cord-008590-xivnsldf 10 15 ” " '' cord-008590-xivnsldf 10 16 function function NN cord-008590-xivnsldf 10 17 of of IN cord-008590-xivnsldf 10 18 the the DT cord-008590-xivnsldf 10 19 GC GC NNP cord-008590-xivnsldf 10 20 yet yet RB cord-008590-xivnsldf 10 21 they -PRON- PRP cord-008590-xivnsldf 10 22 are be VBP cord-008590-xivnsldf 10 23 crucial crucial JJ cord-008590-xivnsldf 10 24 in in IN cord-008590-xivnsldf 10 25 allowing allow VBG cord-008590-xivnsldf 10 26 the the DT cord-008590-xivnsldf 10 27 cell cell NN cord-008590-xivnsldf 10 28 to to TO cord-008590-xivnsldf 10 29 tailor tailor VB cord-008590-xivnsldf 10 30 its -PRON- PRP$ cord-008590-xivnsldf 10 31 biosynthetic biosynthetic JJ cord-008590-xivnsldf 10 32 products product NNS cord-008590-xivnsldf 10 33 for for IN cord-008590-xivnsldf 10 34 its -PRON- PRP$ cord-008590-xivnsldf 10 35 own own JJ cord-008590-xivnsldf 10 36 needs need NNS cord-008590-xivnsldf 10 37 and and CC cord-008590-xivnsldf 10 38 the the DT cord-008590-xivnsldf 10 39 needs need NNS cord-008590-xivnsldf 10 40 of of IN cord-008590-xivnsldf 10 41 the the DT cord-008590-xivnsldf 10 42 organism organism NN cord-008590-xivnsldf 10 43 as as IN cord-008590-xivnsldf 10 44 a a DT cord-008590-xivnsldf 10 45 whole whole NN cord-008590-xivnsldf 10 46 . . . cord-008590-xivnsldf 11 1 In in IN cord-008590-xivnsldf 11 2 addition addition NN cord-008590-xivnsldf 11 3 to to IN cord-008590-xivnsldf 11 4 modifying modify VBG cord-008590-xivnsldf 11 5 products product NNS cord-008590-xivnsldf 11 6 of of IN cord-008590-xivnsldf 11 7 the the DT cord-008590-xivnsldf 11 8 rough rough JJ cord-008590-xivnsldf 11 9 endoplasmic endoplasmic NN cord-008590-xivnsldf 11 10 reticulum reticulum NN cord-008590-xivnsldf 11 11 , , , cord-008590-xivnsldf 11 12 the the DT cord-008590-xivnsldf 11 13 GC GC NNP cord-008590-xivnsldf 11 14 may may MD cord-008590-xivnsldf 11 15 be be VB cord-008590-xivnsldf 11 16 involved involve VBN cord-008590-xivnsldf 11 17 in in IN cord-008590-xivnsldf 11 18 processing process VBG cord-008590-xivnsldf 11 19 events event NNS cord-008590-xivnsldf 11 20 because because IN cord-008590-xivnsldf 11 21 of of IN cord-008590-xivnsldf 11 22 its -PRON- PRP$ cord-008590-xivnsldf 11 23 participation participation NN cord-008590-xivnsldf 11 24 in in IN cord-008590-xivnsldf 11 25 other other JJ cord-008590-xivnsldf 11 26 routes route NNS cord-008590-xivnsldf 11 27 of of IN cord-008590-xivnsldf 11 28 vesicular vesicular JJ cord-008590-xivnsldf 11 29 traffic traffic NN cord-008590-xivnsldf 11 30 — — : cord-008590-xivnsldf 11 31 for for IN cord-008590-xivnsldf 11 32 example example NN cord-008590-xivnsldf 11 33 , , , cord-008590-xivnsldf 11 34 centripetal centripetal JJ cord-008590-xivnsldf 11 35 traffic traffic NN cord-008590-xivnsldf 11 36 from from IN cord-008590-xivnsldf 11 37 the the DT cord-008590-xivnsldf 11 38 cell cell NN cord-008590-xivnsldf 11 39 surface surface NN cord-008590-xivnsldf 11 40 . . . cord-008590-xivnsldf 12 1 Various various JJ cord-008590-xivnsldf 12 2 nonequivalent nonequivalent JJ cord-008590-xivnsldf 12 3 criteria criterion NNS cord-008590-xivnsldf 12 4 have have VBP cord-008590-xivnsldf 12 5 been be VBN cord-008590-xivnsldf 12 6 used use VBN cord-008590-xivnsldf 12 7 to to TO cord-008590-xivnsldf 12 8 ascribe ascribe VB cord-008590-xivnsldf 12 9 processing processing NN cord-008590-xivnsldf 12 10 events event NNS cord-008590-xivnsldf 12 11 to to IN cord-008590-xivnsldf 12 12 the the DT cord-008590-xivnsldf 12 13 GC GC NNP cord-008590-xivnsldf 12 14 - - HYPH cord-008590-xivnsldf 12 15 autoradiography autoradiography NNP cord-008590-xivnsldf 12 16 , , , cord-008590-xivnsldf 12 17 preparative preparative JJ cord-008590-xivnsldf 12 18 or or CC cord-008590-xivnsldf 12 19 analytic analytic JJ cord-008590-xivnsldf 12 20 subcellular subcellular JJ cord-008590-xivnsldf 12 21 fractionation fractionation NN cord-008590-xivnsldf 12 22 , , , cord-008590-xivnsldf 12 23 interruption interruption NN cord-008590-xivnsldf 12 24 by by IN cord-008590-xivnsldf 12 25 ICT ICT NNP cord-008590-xivnsldf 12 26 inhibitors inhibitor NNS cord-008590-xivnsldf 12 27 , , , cord-008590-xivnsldf 12 28 and and CC cord-008590-xivnsldf 12 29 delay delay VB cord-008590-xivnsldf 12 30 in in IN cord-008590-xivnsldf 12 31 the the DT cord-008590-xivnsldf 12 32 impact impact NN cord-008590-xivnsldf 12 33 of of IN cord-008590-xivnsldf 12 34 cycloheximide cycloheximide NN cord-008590-xivnsldf 12 35 . . . cord-008590-xivnsldf 13 1 The the DT cord-008590-xivnsldf 13 2 organized organized JJ cord-008590-xivnsldf 13 3 and and CC cord-008590-xivnsldf 13 4 characteristic characteristic JJ cord-008590-xivnsldf 13 5 elements element NNS cord-008590-xivnsldf 13 6 of of IN cord-008590-xivnsldf 13 7 the the DT cord-008590-xivnsldf 13 8 Golgi Golgi NNP cord-008590-xivnsldf 13 9 complex complex NN cord-008590-xivnsldf 13 10 ( ( -LRB- cord-008590-xivnsldf 13 11 GC GC NNP cord-008590-xivnsldf 13 12 ) ) -RRB- cord-008590-xivnsldf 13 13 ' ' '' cord-008590-xivnsldf 13 14 are be VBP cord-008590-xivnsldf 13 15 the the DT cord-008590-xivnsldf 13 16 stacked stack VBN cord-008590-xivnsldf 13 17 smooth smooth RB cord-008590-xivnsldf 13 18 - - HYPH cord-008590-xivnsldf 13 19 surfaced surface VBN cord-008590-xivnsldf 13 20 cisternae cisternae NN cord-008590-xivnsldf 13 21 which which WDT cord-008590-xivnsldf 13 22 are be VBP cord-008590-xivnsldf 13 23 found find VBN cord-008590-xivnsldf 13 24 in in IN cord-008590-xivnsldf 13 25 the the DT cord-008590-xivnsldf 13 26 centrosphere centrosphere NN cord-008590-xivnsldf 13 27 of of IN cord-008590-xivnsldf 13 28 all all DT cord-008590-xivnsldf 13 29 eukaryotic eukaryotic JJ cord-008590-xivnsldf 13 30 cells cell NNS cord-008590-xivnsldf 13 31 . . . cord-008590-xivnsldf 14 1 These these DT cord-008590-xivnsldf 14 2 cisternae cisternae NNP cord-008590-xivnsldf 14 3 , , , cord-008590-xivnsldf 14 4 in in IN cord-008590-xivnsldf 14 5 conjunction conjunction NN cord-008590-xivnsldf 14 6 with with IN cord-008590-xivnsldf 14 7 other other JJ cord-008590-xivnsldf 14 8 associated associate VBN cord-008590-xivnsldf 14 9 smoothsurfaced smoothsurface VBN cord-008590-xivnsldf 14 10 membranes membrane NNS cord-008590-xivnsldf 14 11 , , , cord-008590-xivnsldf 14 12 are be VBP cord-008590-xivnsldf 14 13 responsible responsible JJ cord-008590-xivnsldf 14 14 for for IN cord-008590-xivnsldf 14 15 executing execute VBG cord-008590-xivnsldf 14 16 net net JJ cord-008590-xivnsldf 14 17 unidirectional unidirectional JJ cord-008590-xivnsldf 14 18 intracellular intracellular JJ cord-008590-xivnsldf 14 19 transport transport NN cord-008590-xivnsldf 14 20 ( ( -LRB- cord-008590-xivnsldf 14 21 ICT ICT NNP cord-008590-xivnsldf 14 22 ) ) -RRB- cord-008590-xivnsldf 14 23 from from IN cord-008590-xivnsldf 14 24 the the DT cord-008590-xivnsldf 14 25 rough rough JJ cord-008590-xivnsldf 14 26 endoplasmic endoplasmic NN cord-008590-xivnsldf 14 27 reticulum reticulum NN cord-008590-xivnsldf 14 28 ( ( -LRB- cord-008590-xivnsldf 14 29 RER RER NNP cord-008590-xivnsldf 14 30 ) ) -RRB- cord-008590-xivnsldf 14 31 toward toward IN cord-008590-xivnsldf 14 32 more more RBR cord-008590-xivnsldf 14 33 distally distally RB cord-008590-xivnsldf 14 34 located locate VBN cord-008590-xivnsldf 14 35 structures structure NNS cord-008590-xivnsldf 14 36 , , , cord-008590-xivnsldf 14 37 e.g. e.g. RB cord-008590-xivnsldf 14 38 , , , cord-008590-xivnsldf 14 39 lysosomes lysosome NNS cord-008590-xivnsldf 14 40 and and CC cord-008590-xivnsldf 14 41 the the DT cord-008590-xivnsldf 14 42 cell cell NN cord-008590-xivnsldf 14 43 surface surface NN cord-008590-xivnsldf 14 44 ( ( -LRB- cord-008590-xivnsldf 14 45 24 24 CD cord-008590-xivnsldf 14 46 , , , cord-008590-xivnsldf 14 47 35 35 CD cord-008590-xivnsldf 14 48 , , , cord-008590-xivnsldf 14 49 40 40 CD cord-008590-xivnsldf 14 50 , , , cord-008590-xivnsldf 14 51 47 47 CD cord-008590-xivnsldf 14 52 , , , cord-008590-xivnsldf 14 53 85 85 CD cord-008590-xivnsldf 14 54 , , , cord-008590-xivnsldf 14 55 1 1 CD cord-008590-xivnsldf 14 56 1 1 CD cord-008590-xivnsldf 14 57 1 1 CD cord-008590-xivnsldf 14 58 , , , cord-008590-xivnsldf 14 59 134 134 CD cord-008590-xivnsldf 14 60 ) ) -RRB- cord-008590-xivnsldf 14 61 . . . cord-008590-xivnsldf 15 1 The the DT cord-008590-xivnsldf 15 2 present present JJ cord-008590-xivnsldf 15 3 article article NN cord-008590-xivnsldf 15 4 is be VBZ cord-008590-xivnsldf 15 5 not not RB cord-008590-xivnsldf 15 6 centered center VBN cord-008590-xivnsldf 15 7 on on IN cord-008590-xivnsldf 15 8 this this DT cord-008590-xivnsldf 15 9 apparently apparently RB cord-008590-xivnsldf 15 10 most most JJS cord-008590-xivnsldf 15 11 primary primary JJ cord-008590-xivnsldf 15 12 of of IN cord-008590-xivnsldf 15 13 Golgi Golgi NNP cord-008590-xivnsldf 15 14 functions function NNS cord-008590-xivnsldf 15 15 , , , cord-008590-xivnsldf 15 16 but but CC cord-008590-xivnsldf 15 17 rather rather RB cord-008590-xivnsldf 15 18 on on IN cord-008590-xivnsldf 15 19 the the DT cord-008590-xivnsldf 15 20 broad broad JJ cord-008590-xivnsldf 15 21 range range NN cord-008590-xivnsldf 15 22 of of IN cord-008590-xivnsldf 15 23 accessory accessory JJ cord-008590-xivnsldf 15 24 activities activity NNS cord-008590-xivnsldf 15 25 which which WDT cord-008590-xivnsldf 15 26 occur occur VBP cord-008590-xivnsldf 15 27 during during IN cord-008590-xivnsldf 15 28 transport transport NN cord-008590-xivnsldf 15 29 , , , cord-008590-xivnsldf 15 30 the the DT cord-008590-xivnsldf 15 31 family family NN cord-008590-xivnsldf 15 32 of of IN cord-008590-xivnsldf 15 33 " " `` cord-008590-xivnsldf 15 34 posttranslational posttranslational JJ cord-008590-xivnsldf 15 35 modifications modification NNS cord-008590-xivnsldf 15 36 . . . cord-008590-xivnsldf 15 37 " " '' cord-008590-xivnsldf 16 1 T T NNP cord-008590-xivnsldf 16 2 k k NNP cord-008590-xivnsldf 16 3 s s NN cord-008590-xivnsldf 17 1 e e LS cord-008590-xivnsldf 17 2 I -PRON- PRP cord-008590-xivnsldf 17 3 . . . cord-008590-xivnsldf 18 1 The the DT cord-008590-xivnsldf 18 2 pharmacologic pharmacologic JJ cord-008590-xivnsldf 18 3 approach approach NN cord-008590-xivnsldf 18 4 which which WDT cord-008590-xivnsldf 18 5 makes make VBZ cord-008590-xivnsldf 18 6 use use NN cord-008590-xivnsldf 18 7 of of IN cord-008590-xivnsldf 18 8 monensin monensin NNP cord-008590-xivnsldf 18 9 to to TO cord-008590-xivnsldf 18 10 interrupt interrupt VB cord-008590-xivnsldf 18 11 ICT ICT NNP cord-008590-xivnsldf 18 12 . . . cord-008590-xivnsldf 19 1 As as IN cord-008590-xivnsldf 19 2 has have VBZ cord-008590-xivnsldf 19 3 been be VBN cord-008590-xivnsldf 19 4 discussed discuss VBN cord-008590-xivnsldf 19 5 elsewhere elsewhere RB cord-008590-xivnsldf 19 6 ( ( -LRB- cord-008590-xivnsldf 19 7 134 134 CD cord-008590-xivnsldf 19 8 ) ) -RRB- cord-008590-xivnsldf 19 9 ( ( -LRB- cord-008590-xivnsldf 19 10 135 135 CD cord-008590-xivnsldf 19 11 ) ) -RRB- cord-008590-xivnsldf 19 12 ( ( -LRB- cord-008590-xivnsldf 19 13 136 136 CD cord-008590-xivnsldf 19 14 ) ) -RRB- cord-008590-xivnsldf 19 15 ( ( -LRB- cord-008590-xivnsldf 19 16 137 137 CD cord-008590-xivnsldf 19 17 ) ) -RRB- cord-008590-xivnsldf 19 18 , , , cord-008590-xivnsldf 19 19 the the DT cord-008590-xivnsldf 19 20 carboxylic carboxylic JJ cord-008590-xivnsldf 19 21 ionophore ionophore NNP cord-008590-xivnsldf 19 22 monensin monensin NNP cord-008590-xivnsldf 19 23 causes cause VBZ cord-008590-xivnsldf 19 24 partial partial JJ cord-008590-xivnsldf 19 25 equilibration equilibration NN cord-008590-xivnsldf 19 26 of of IN cord-008590-xivnsldf 19 27 intra intra JJ cord-008590-xivnsldf 19 28 - - : cord-008590-xivnsldf 19 29 and and CC cord-008590-xivnsldf 19 30 extracellular extracellular JJ cord-008590-xivnsldf 19 31 Na+ na+ NN cord-008590-xivnsldf 19 32 and and CC cord-008590-xivnsldf 19 33 K K NNP cord-008590-xivnsldf 20 1 + + CC cord-008590-xivnsldf 20 2 , , , cord-008590-xivnsldf 20 3 rapid rapid JJ cord-008590-xivnsldf 20 4 ( ( -LRB- cord-008590-xivnsldf 20 5 within within IN cord-008590-xivnsldf 20 6 seconds second NNS cord-008590-xivnsldf 20 7 ) ) -RRB- cord-008590-xivnsldf 20 8 dilation dilation NN cord-008590-xivnsldf 20 9 of of IN cord-008590-xivnsldf 20 10 all all DT cord-008590-xivnsldf 20 11 Golgi Golgi NNP cord-008590-xivnsldf 20 12 cisternae cisternae NNP cord-008590-xivnsldf 20 13 , , , cord-008590-xivnsldf 20 14 and and CC cord-008590-xivnsldf 20 15 an an DT cord-008590-xivnsldf 20 16 impressive impressive JJ cord-008590-xivnsldf 20 17 slowing slowing NN cord-008590-xivnsldf 20 18 of of IN cord-008590-xivnsldf 20 19 ICT ICT NNP cord-008590-xivnsldf 20 20 . . . cord-008590-xivnsldf 21 1 The the DT cord-008590-xivnsldf 21 2 agent agent NN cord-008590-xivnsldf 21 3 is be VBZ cord-008590-xivnsldf 21 4 highly highly RB cord-008590-xivnsldf 21 5 selective selective JJ cord-008590-xivnsldf 21 6 , , , cord-008590-xivnsldf 21 7 at at IN cord-008590-xivnsldf 21 8 least least JJS cord-008590-xivnsldf 21 9 after after IN cord-008590-xivnsldf 21 10 brief brief JJ cord-008590-xivnsldf 21 11 treatment treatment NN cord-008590-xivnsldf 21 12 ( ( -LRB- cord-008590-xivnsldf 21 13 1 1 CD cord-008590-xivnsldf 21 14 pJ4 pJ4 NNP cord-008590-xivnsldf 21 15 monensin monensin NNP cord-008590-xivnsldf 21 16 , , , cord-008590-xivnsldf 21 17 1 1 CD cord-008590-xivnsldf 21 18 hour hour NN cord-008590-xivnsldf 21 19 , , , cord-008590-xivnsldf 21 20 37 37 CD cord-008590-xivnsldf 21 21 ° ° NN cord-008590-xivnsldf 21 22 C c NN cord-008590-xivnsldf 21 23 ) ) -RRB- cord-008590-xivnsldf 21 24 : : : cord-008590-xivnsldf 21 25 ATP ATP NNP cord-008590-xivnsldf 21 26 levels level NNS cord-008590-xivnsldf 21 27 and and CC cord-008590-xivnsldf 21 28 intracellular intracellular JJ cord-008590-xivnsldf 21 29 pH pH NNP cord-008590-xivnsldf 21 30 are be VBP cord-008590-xivnsldf 21 31 little little RB cord-008590-xivnsldf 21 32 changed change VBN cord-008590-xivnsldf 21 33 , , , cord-008590-xivnsldf 21 34 many many JJ cord-008590-xivnsldf 21 35 biosynthetic biosynthetic JJ cord-008590-xivnsldf 21 36 events event NNS cord-008590-xivnsldf 21 37 continue continue VBP cord-008590-xivnsldf 21 38 [ [ -LRB- cord-008590-xivnsldf 21 39 protein protein NN cord-008590-xivnsldf 21 40 synthesis synthesis NN cord-008590-xivnsldf 21 41 , , , cord-008590-xivnsldf 21 42 lipid lipid NN cord-008590-xivnsldf 21 43 addition addition NN cord-008590-xivnsldf 21 44 to to IN cord-008590-xivnsldf 21 45 glycoproteins glycoprotein NNS cord-008590-xivnsldf 21 46 ( ( -LRB- cord-008590-xivnsldf 21 47 54 54 CD cord-008590-xivnsldf 21 48 ) ) -RRB- cord-008590-xivnsldf 21 49 hyaluronic hyaluronic NN cord-008590-xivnsldf 21 50 acid acid NN cord-008590-xivnsldf 21 51 synthesis synthesis NN cord-008590-xivnsldf 21 52 ( ( -LRB- cord-008590-xivnsldf 21 53 84 84 CD cord-008590-xivnsldf 21 54 ) ) -RRB- cord-008590-xivnsldf 21 55 etc etc FW cord-008590-xivnsldf 21 56 . . . cord-008590-xivnsldf 21 57 ] ] -RRB- cord-008590-xivnsldf 21 58 , , , cord-008590-xivnsldf 21 59 and and CC cord-008590-xivnsldf 21 60 the the DT cord-008590-xivnsldf 21 61 effects effect NNS cord-008590-xivnsldf 21 62 of of IN cord-008590-xivnsldf 21 63 the the DT cord-008590-xivnsldf 21 64 ionophore ionophore NNP cord-008590-xivnsldf 21 65 are be VBP cord-008590-xivnsldf 21 66 reversible reversible JJ cord-008590-xivnsldf 21 67 . . . cord-008590-xivnsldf 22 1 * * NFP cord-008590-xivnsldf 22 2 Since since IN cord-008590-xivnsldf 22 3 in in IN cord-008590-xivnsldf 22 4 the the DT cord-008590-xivnsldf 22 5 presence presence NN cord-008590-xivnsldf 22 6 of of IN cord-008590-xivnsldf 22 7 monensin monensin NNP cord-008590-xivnsldf 22 8 newly newly RB cord-008590-xivnsldf 22 9 synthesized synthesize VBN cord-008590-xivnsldf 22 10 proteins protein NNS cord-008590-xivnsldf 22 11 continue continue VBP cord-008590-xivnsldf 22 12 to to TO cord-008590-xivnsldf 22 13 exit exit VB cord-008590-xivnsldf 22 14 from from IN cord-008590-xivnsldf 22 15 the the DT cord-008590-xivnsldf 22 16 rough rough JJ cord-008590-xivnsldf 22 17 endoplasmic endoplasmic NN cord-008590-xivnsldf 22 18 reticulum reticulum NN cord-008590-xivnsldf 22 19 ( ( -LRB- cord-008590-xivnsldf 22 20 RER RER NNP cord-008590-xivnsldf 22 21 ) ) -RRB- cord-008590-xivnsldf 22 22 , , , cord-008590-xivnsldf 22 23 and and CC cord-008590-xivnsldf 22 24 gain gain VB cord-008590-xivnsldf 22 25 access access NN cord-008590-xivnsldf 22 26 to to IN cord-008590-xivnsldf 22 27 but but CC cord-008590-xivnsldf 22 28 do do VBP cord-008590-xivnsldf 22 29 not not RB cord-008590-xivnsldf 22 30 traverse traverse VB cord-008590-xivnsldf 22 31 the the DT cord-008590-xivnsldf 22 32 GC GC NNP cord-008590-xivnsldf 22 33 , , , cord-008590-xivnsldf 22 34 one one PRP cord-008590-xivnsldf 22 35 may may MD cord-008590-xivnsldf 22 36 inquire inquire VB cord-008590-xivnsldf 22 37 whether whether IN cord-008590-xivnsldf 22 38 given give VBN cord-008590-xivnsldf 22 39 posttranslational posttranslational JJ cord-008590-xivnsldf 22 40 modifications modification NNS cord-008590-xivnsldf 22 41 occur occur VBP cord-008590-xivnsldf 22 42 . . . cord-008590-xivnsldf 23 1 If if IN cord-008590-xivnsldf 23 2 they -PRON- PRP cord-008590-xivnsldf 23 3 do do VBP cord-008590-xivnsldf 23 4 , , , cord-008590-xivnsldf 23 5 they -PRON- PRP cord-008590-xivnsldf 23 6 may may MD cord-008590-xivnsldf 23 7 be be VB cord-008590-xivnsldf 23 8 assigned assign VBN cord-008590-xivnsldf 23 9 to to IN cord-008590-xivnsldf 23 10 a a DT cord-008590-xivnsldf 23 11 relatively relatively RB cord-008590-xivnsldf 23 12 early early JJ cord-008590-xivnsldf 23 13 subcompartment subcompartment NN cord-008590-xivnsldf 23 14 . . . cord-008590-xivnsldf 24 1 If if IN cord-008590-xivnsldf 24 2 they -PRON- PRP cord-008590-xivnsldf 24 3 do do VBP cord-008590-xivnsldf 24 4 not not RB cord-008590-xivnsldf 24 5 , , , cord-008590-xivnsldf 24 6 given give VBN cord-008590-xivnsldf 24 7 appropriate appropriate JJ cord-008590-xivnsldf 24 8 control control NN cord-008590-xivnsldf 24 9 experiments experiment NNS cord-008590-xivnsldf 24 10 , , , cord-008590-xivnsldf 24 11 they -PRON- PRP cord-008590-xivnsldf 24 12 may may MD cord-008590-xivnsldf 24 13 be be VB cord-008590-xivnsldf 24 14 assigned assign VBN cord-008590-xivnsldf 24 15 to to IN cord-008590-xivnsldf 24 16 relatively relatively RB cord-008590-xivnsldf 24 17 late late JJ cord-008590-xivnsldf 24 18 sub sub NN cord-008590-xivnsldf 24 19 compartment^.^ compartment^.^ JJ cord-008590-xivnsldf 25 1 The the DT cord-008590-xivnsldf 25 2 first first JJ cord-008590-xivnsldf 25 3 indications indication NNS cord-008590-xivnsldf 25 4 that that IN cord-008590-xivnsldf 25 5 the the DT cord-008590-xivnsldf 25 6 site site NN cord-008590-xivnsldf 25 7 of of IN cord-008590-xivnsldf 25 8 interruption interruption NN cord-008590-xivnsldf 25 9 of of IN cord-008590-xivnsldf 25 10 ICT ICT NNP cord-008590-xivnsldf 25 11 by by IN cord-008590-xivnsldf 25 12 monensin monensin NNP cord-008590-xivnsldf 25 13 lies lie VBZ cord-008590-xivnsldf 25 14 only only RB cord-008590-xivnsldf 25 15 part part NN cord-008590-xivnsldf 25 16 way way NN cord-008590-xivnsldf 25 17 across across IN cord-008590-xivnsldf 25 18 the the DT cord-008590-xivnsldf 25 19 GC GC NNP cord-008590-xivnsldf 25 20 came come VBD cord-008590-xivnsldf 25 21 from from IN cord-008590-xivnsldf 25 22 study study NN cord-008590-xivnsldf 25 23 of of IN cord-008590-xivnsldf 25 24 the the DT cord-008590-xivnsldf 25 25 progress progress NN cord-008590-xivnsldf 25 26 of of IN cord-008590-xivnsldf 25 27 N n NN cord-008590-xivnsldf 25 28 - - HYPH cord-008590-xivnsldf 25 29 linked link VBN cord-008590-xivnsldf 25 30 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 25 31 maturation maturation NN cord-008590-xivnsldf 25 32 of of IN cord-008590-xivnsldf 25 33 Ig Ig NNP cord-008590-xivnsldf 25 34 ( ( -LRB- cord-008590-xivnsldf 25 35 139 139 CD cord-008590-xivnsldf 25 36 ) ) -RRB- cord-008590-xivnsldf 25 37 ; ; : cord-008590-xivnsldf 25 38 however however RB cord-008590-xivnsldf 25 39 , , , cord-008590-xivnsldf 25 40 a a DT cord-008590-xivnsldf 25 41 recent recent JJ cord-008590-xivnsldf 25 42 use use NN cord-008590-xivnsldf 25 43 of of IN cord-008590-xivnsldf 25 44 monensin monensin NNP cord-008590-xivnsldf 25 45 should should MD cord-008590-xivnsldf 25 46 be be VB cord-008590-xivnsldf 25 47 mentioned mention VBN cord-008590-xivnsldf 25 48 since since IN cord-008590-xivnsldf 25 49 it -PRON- PRP cord-008590-xivnsldf 25 50 adds add VBZ cord-008590-xivnsldf 25 51 independent independent JJ cord-008590-xivnsldf 25 52 support support NN cord-008590-xivnsldf 25 53 for for IN cord-008590-xivnsldf 25 54 this this DT cord-008590-xivnsldf 25 55 idea idea NN cord-008590-xivnsldf 25 56 ( ( -LRB- cord-008590-xivnsldf 25 57 37 37 CD cord-008590-xivnsldf 25 58 ) ) -RRB- cord-008590-xivnsldf 25 59 . . . cord-008590-xivnsldf 26 1 When when WRB cord-008590-xivnsldf 26 2 BHK BHK NNP cord-008590-xivnsldf 26 3 cells cell NNS cord-008590-xivnsldf 26 4 are be VBP cord-008590-xivnsldf 26 5 infected infect VBN cord-008590-xivnsldf 26 6 with with IN cord-008590-xivnsldf 26 7 Sindbis Sindbis NNP cord-008590-xivnsldf 26 8 or or CC cord-008590-xivnsldf 26 9 Semliki Semliki NNP cord-008590-xivnsldf 26 10 Forest Forest NNP cord-008590-xivnsldf 26 11 virus virus NN cord-008590-xivnsldf 26 12 , , , cord-008590-xivnsldf 26 13 viral viral JJ cord-008590-xivnsldf 26 14 budding budding NN cord-008590-xivnsldf 26 15 is be VBZ cord-008590-xivnsldf 26 16 normally normally RB cord-008590-xivnsldf 26 17 observed observe VBN cord-008590-xivnsldf 26 18 at at IN cord-008590-xivnsldf 26 19 the the DT cord-008590-xivnsldf 26 20 cell cell NN cord-008590-xivnsldf 26 21 surface surface NN cord-008590-xivnsldf 26 22 . . . cord-008590-xivnsldf 27 1 If if IN cord-008590-xivnsldf 27 2 monensin monensin NNP cord-008590-xivnsldf 27 3 is be VBZ cord-008590-xivnsldf 27 4 present present JJ cord-008590-xivnsldf 27 5 , , , cord-008590-xivnsldf 27 6 transport transport NN cord-008590-xivnsldf 27 7 of of IN cord-008590-xivnsldf 27 8 the the DT cord-008590-xivnsldf 27 9 glycoproteins glycoprotein NNS cord-008590-xivnsldf 27 10 is be VBZ cord-008590-xivnsldf 27 11 interrupted interrupt VBN cord-008590-xivnsldf 27 12 in in IN cord-008590-xivnsldf 27 13 the the DT cord-008590-xivnsldf 27 14 GC GC NNP cord-008590-xivnsldf 27 15 ( ( -LRB- cord-008590-xivnsldf 27 16 37 37 CD cord-008590-xivnsldf 27 17 , , , cord-008590-xivnsldf 27 18 54 54 CD cord-008590-xivnsldf 27 19 , , , cord-008590-xivnsldf 27 20 56 56 CD cord-008590-xivnsldf 27 21 ) ) -RRB- cord-008590-xivnsldf 27 22 and and CC cord-008590-xivnsldf 27 23 viral viral JJ cord-008590-xivnsldf 27 24 nucleocapsids nucleocapsid NNS cord-008590-xivnsldf 27 25 are be VBP cord-008590-xivnsldf 27 26 found find VBN cord-008590-xivnsldf 27 27 adhering adhere VBG cord-008590-xivnsldf 27 28 to to IN cord-008590-xivnsldf 27 29 and and CC cord-008590-xivnsldf 27 30 budding bud VBG cord-008590-xivnsldf 27 31 across across IN cord-008590-xivnsldf 27 32 the the DT cord-008590-xivnsldf 27 33 membranes membrane NNS cord-008590-xivnsldf 27 34 of of IN cord-008590-xivnsldf 27 35 dilated dilated JJ cord-008590-xivnsldf 27 36 vacuoles vacuole NNS cord-008590-xivnsldf 27 37 ( ( -LRB- cord-008590-xivnsldf 27 38 " " `` cord-008590-xivnsldf 27 39 ICBMs ICBMs NNP cord-008590-xivnsldf 27 40 " " '' cord-008590-xivnsldf 27 41 ) ) -RRB- cord-008590-xivnsldf 27 42 in in IN cord-008590-xivnsldf 27 43 the the DT cord-008590-xivnsldf 27 44 Golgi Golgi NNP cord-008590-xivnsldf 27 45 region region NN cord-008590-xivnsldf 27 46 . . . cord-008590-xivnsldf 28 1 These these DT cord-008590-xivnsldf 28 2 nucleocapsids nucleocapsid NNS cord-008590-xivnsldf 28 3 are be VBP cord-008590-xivnsldf 28 4 presum-*In presum-*In VBG cord-008590-xivnsldf 28 5 the the DT cord-008590-xivnsldf 28 6 context context NN cord-008590-xivnsldf 28 7 of of IN cord-008590-xivnsldf 28 8 the the DT cord-008590-xivnsldf 28 9 later later JJ cord-008590-xivnsldf 28 10 discussion discussion NN cord-008590-xivnsldf 28 11 , , , cord-008590-xivnsldf 28 12 the the DT cord-008590-xivnsldf 28 13 study study NN cord-008590-xivnsldf 28 14 of of IN cord-008590-xivnsldf 28 15 hyaluronic hyaluronic NN cord-008590-xivnsldf 28 16 acid acid NN cord-008590-xivnsldf 28 17 biosynthesis biosynthesis NN cord-008590-xivnsldf 28 18 is be VBZ cord-008590-xivnsldf 28 19 especially especially RB cord-008590-xivnsldf 28 20 important important JJ cord-008590-xivnsldf 28 21 since since IN cord-008590-xivnsldf 28 22 it -PRON- PRP cord-008590-xivnsldf 28 23 strongly strongly RB cord-008590-xivnsldf 28 24 suggests suggest VBZ cord-008590-xivnsldf 28 25 that that IN cord-008590-xivnsldf 28 26 sugar sugar NN cord-008590-xivnsldf 28 27 nucleotide nucleotide JJ cord-008590-xivnsldf 28 28 metabolism metabolism NN cord-008590-xivnsldf 28 29 is be VBZ cord-008590-xivnsldf 28 30 little little RB cord-008590-xivnsldf 28 31 perturbed perturbed JJ cord-008590-xivnsldf 28 32 and and CC cord-008590-xivnsldf 28 33 that that IN cord-008590-xivnsldf 28 34 the the DT cord-008590-xivnsldf 28 35 effects effect NNS cord-008590-xivnsldf 28 36 of of IN cord-008590-xivnsldf 28 37 monensin monensin NNP cord-008590-xivnsldf 28 38 on on IN cord-008590-xivnsldf 28 39 sugar sugar NN cord-008590-xivnsldf 28 40 incorporation incorporation NN cord-008590-xivnsldf 28 41 ( ( -LRB- cord-008590-xivnsldf 28 42 e.g. e.g. RB cord-008590-xivnsldf 28 43 , , , cord-008590-xivnsldf 28 44 into into IN cord-008590-xivnsldf 28 45 glycoproteins glycoprotein NNS cord-008590-xivnsldf 28 46 ) ) -RRB- cord-008590-xivnsldf 28 47 are be VBP cord-008590-xivnsldf 28 48 directly directly RB cord-008590-xivnsldf 28 49 related relate VBN cord-008590-xivnsldf 28 50 to to IN cord-008590-xivnsldf 28 51 intermption intermption NN cord-008590-xivnsldf 28 52 of of IN cord-008590-xivnsldf 28 53 ICT ICT NNP cord-008590-xivnsldf 28 54 . . . cord-008590-xivnsldf 29 1 Hyaluronic hyaluronic NN cord-008590-xivnsldf 29 2 acid acid NN cord-008590-xivnsldf 29 3 , , , cord-008590-xivnsldf 29 4 since since IN cord-008590-xivnsldf 29 5 it -PRON- PRP cord-008590-xivnsldf 29 6 lacks lack VBZ cord-008590-xivnsldf 29 7 a a DT cord-008590-xivnsldf 29 8 protein protein NN cord-008590-xivnsldf 29 9 core core NN cord-008590-xivnsldf 29 10 , , , cord-008590-xivnsldf 29 11 would would MD cord-008590-xivnsldf 29 12 be be VB cord-008590-xivnsldf 29 13 free free JJ cord-008590-xivnsldf 29 14 from from IN cord-008590-xivnsldf 29 15 such such JJ cord-008590-xivnsldf 29 16 effects effect NNS cord-008590-xivnsldf 29 17 . . . cord-008590-xivnsldf 30 1 3The 3the CD cord-008590-xivnsldf 30 2 appropriate appropriate JJ cord-008590-xivnsldf 30 3 controls control NNS cord-008590-xivnsldf 30 4 vary vary VBP cord-008590-xivnsldf 30 5 according accord VBG cord-008590-xivnsldf 30 6 to to IN cord-008590-xivnsldf 30 7 the the DT cord-008590-xivnsldf 30 8 event event NN cord-008590-xivnsldf 30 9 being be VBG cord-008590-xivnsldf 30 10 studied study VBN cord-008590-xivnsldf 30 11 . . . cord-008590-xivnsldf 31 1 For for IN cord-008590-xivnsldf 31 2 example example NN cord-008590-xivnsldf 31 3 , , , cord-008590-xivnsldf 31 4 in in IN cord-008590-xivnsldf 31 5 the the DT cord-008590-xivnsldf 31 6 case case NN cord-008590-xivnsldf 31 7 of of IN cord-008590-xivnsldf 31 8 Ig Ig NNP cord-008590-xivnsldf 31 9 carbohydrate carbohydrate NN cord-008590-xivnsldf 31 10 maturation maturation NN cord-008590-xivnsldf 31 11 although although IN cord-008590-xivnsldf 31 12 monensin monensin NNP cord-008590-xivnsldf 31 13 blocks block VBZ cord-008590-xivnsldf 31 14 I3H]Gal I3H]Gal NNP cord-008590-xivnsldf 31 15 and and CC cord-008590-xivnsldf 31 16 [ [ -LRB- cord-008590-xivnsldf 31 17 3H]Fuc 3h]fuc CD cord-008590-xivnsldf 31 18 labeling labeling NN cord-008590-xivnsldf 31 19 of of IN cord-008590-xivnsldf 31 20 Ig Ig NNP cord-008590-xivnsldf 31 21 , , , cord-008590-xivnsldf 31 22 the the DT cord-008590-xivnsldf 31 23 two two CD cord-008590-xivnsldf 31 24 isotopes isotope NNS cord-008590-xivnsldf 31 25 continue continue VBP cord-008590-xivnsldf 31 26 to to TO cord-008590-xivnsldf 31 27 be be VB cord-008590-xivnsldf 31 28 incorporated incorporate VBN cord-008590-xivnsldf 31 29 into into IN cord-008590-xivnsldf 31 30 other other JJ cord-008590-xivnsldf 31 31 acid acid NN cord-008590-xivnsldf 31 32 - - HYPH cord-008590-xivnsldf 31 33 insoluble insoluble JJ cord-008590-xivnsldf 31 34 material material NN cord-008590-xivnsldf 31 35 ( ( -LRB- cord-008590-xivnsldf 31 36 139 139 CD cord-008590-xivnsldf 31 37 ) ) -RRB- cord-008590-xivnsldf 31 38 . . . cord-008590-xivnsldf 32 1 In in IN cord-008590-xivnsldf 32 2 the the DT cord-008590-xivnsldf 32 3 case case NN cord-008590-xivnsldf 32 4 of of IN cord-008590-xivnsldf 32 5 the the DT cord-008590-xivnsldf 32 6 inhibition inhibition NN cord-008590-xivnsldf 32 7 of of IN cord-008590-xivnsldf 32 8 acquisition acquisition NN cord-008590-xivnsldf 32 9 of of IN cord-008590-xivnsldf 32 10 endo endo NNP cord-008590-xivnsldf 32 11 H H NNP cord-008590-xivnsldf 32 12 resistence resistence NN cord-008590-xivnsldf 32 13 by by IN cord-008590-xivnsldf 32 14 transfenin transfenin NNS cord-008590-xivnsldf 32 15 in in IN cord-008590-xivnsldf 32 16 VSV VSV NNP cord-008590-xivnsldf 32 17 - - HYPH cord-008590-xivnsldf 32 18 infected infect VBN cord-008590-xivnsldf 32 19 hepatoma hepatoma NNP cord-008590-xivnsldf 32 20 cells cell NNS cord-008590-xivnsldf 32 21 , , , cord-008590-xivnsldf 32 22 an an DT cord-008590-xivnsldf 32 23 internal internal JJ cord-008590-xivnsldf 32 24 control control NN cord-008590-xivnsldf 32 25 is be VBZ cord-008590-xivnsldf 32 26 provided provide VBN cord-008590-xivnsldf 32 27 by by IN cord-008590-xivnsldf 32 28 the the DT cord-008590-xivnsldf 32 29 G g NN cord-008590-xivnsldf 32 30 protein protein NN cord-008590-xivnsldf 32 31 , , , cord-008590-xivnsldf 32 32 which which WDT cord-008590-xivnsldf 32 33 does do VBZ cord-008590-xivnsldf 32 34 become become VB cord-008590-xivnsldf 32 35 endo endo NN cord-008590-xivnsldf 32 36 H h NN cord-008590-xivnsldf 32 37 resistant resistant JJ cord-008590-xivnsldf 32 38 ( ( -LRB- cord-008590-xivnsldf 32 39 133 133 CD cord-008590-xivnsldf 32 40 ) ) -RRB- cord-008590-xivnsldf 32 41 . . . cord-008590-xivnsldf 33 1 Were be VBD cord-008590-xivnsldf 33 2 posttranslation posttranslation NN cord-008590-xivnsldf 33 3 modifications modification NNS cord-008590-xivnsldf 33 4 due due JJ cord-008590-xivnsldf 33 5 to to IN cord-008590-xivnsldf 33 6 enzymes enzyme NNS cord-008590-xivnsldf 33 7 with with IN cord-008590-xivnsldf 33 8 acid acid NN cord-008590-xivnsldf 33 9 pH pH NNP cord-008590-xivnsldf 33 10 optima optima NNP cord-008590-xivnsldf 33 11 , , , cord-008590-xivnsldf 33 12 inhibition inhibition NN cord-008590-xivnsldf 33 13 by by IN cord-008590-xivnsldf 33 14 monensin monensin NNP cord-008590-xivnsldf 33 15 might may MD cord-008590-xivnsldf 33 16 be be VB cord-008590-xivnsldf 33 17 due due JJ cord-008590-xivnsldf 33 18 to to IN cord-008590-xivnsldf 33 19 alkalinization alkalinization NN cord-008590-xivnsldf 33 20 of of IN cord-008590-xivnsldf 33 21 the the DT cord-008590-xivnsldf 33 22 cistemal cistemal JJ cord-008590-xivnsldf 33 23 content content NN cord-008590-xivnsldf 33 24 resulting result VBG cord-008590-xivnsldf 33 25 from from IN cord-008590-xivnsldf 33 26 H+-Na+ H+-Na+ NNP cord-008590-xivnsldf 33 27 ( ( -LRB- cord-008590-xivnsldf 33 28 or or CC cord-008590-xivnsldf 33 29 K K NNP cord-008590-xivnsldf 33 30 + + SYM cord-008590-xivnsldf 33 31 ) ) -RRB- cord-008590-xivnsldf 33 32 exchange exchange NN cord-008590-xivnsldf 33 33 . . . cord-008590-xivnsldf 34 1 However however RB cord-008590-xivnsldf 34 2 , , , cord-008590-xivnsldf 34 3 all all DT cord-008590-xivnsldf 34 4 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 34 5 processing processing NN cord-008590-xivnsldf 34 6 enzymes enzyme NNS cord-008590-xivnsldf 34 7 studied study VBN cord-008590-xivnsldf 34 8 have have VBP cord-008590-xivnsldf 34 9 roughly roughly RB cord-008590-xivnsldf 34 10 neutral neutral JJ cord-008590-xivnsldf 34 11 pH pH NNP cord-008590-xivnsldf 34 12 optima optima NNP cord-008590-xivnsldf 34 13 . . . cord-008590-xivnsldf 35 1 Furthermore furthermore RB cord-008590-xivnsldf 35 2 , , , cord-008590-xivnsldf 35 3 there there EX cord-008590-xivnsldf 35 4 are be VBP cord-008590-xivnsldf 35 5 no no DT cord-008590-xivnsldf 35 6 indications indication NNS cord-008590-xivnsldf 35 7 that that IN cord-008590-xivnsldf 35 8 they -PRON- PRP cord-008590-xivnsldf 35 9 are be VBP cord-008590-xivnsldf 35 10 sensitive sensitive JJ cord-008590-xivnsldf 35 11 to to IN cord-008590-xivnsldf 35 12 relative relative JJ cord-008590-xivnsldf 35 13 N N NNP cord-008590-xivnsldf 35 14 a a NN cord-008590-xivnsldf 35 15 + + NN cord-008590-xivnsldf 35 16 / / SYM cord-008590-xivnsldf 35 17 K k NN cord-008590-xivnsldf 35 18 + + CC cord-008590-xivnsldf 35 19 levels level NNS cord-008590-xivnsldf 35 20 . . . cord-008590-xivnsldf 36 1 To to IN cord-008590-xivnsldf 36 2 date date NN cord-008590-xivnsldf 36 3 the the DT cord-008590-xivnsldf 36 4 investigators investigator NNS cord-008590-xivnsldf 36 5 who who WP cord-008590-xivnsldf 36 6 use use VBP cord-008590-xivnsldf 36 7 monensin monensin NNP cord-008590-xivnsldf 36 8 unfortunately unfortunately RB cord-008590-xivnsldf 36 9 do do VBP cord-008590-xivnsldf 36 10 not not RB cord-008590-xivnsldf 36 11 all all RB cord-008590-xivnsldf 36 12 conform conform VB cord-008590-xivnsldf 36 13 to to IN cord-008590-xivnsldf 36 14 the the DT cord-008590-xivnsldf 36 15 rather rather RB cord-008590-xivnsldf 36 16 short short JJ cord-008590-xivnsldf 36 17 - - HYPH cord-008590-xivnsldf 36 18 term term NN cord-008590-xivnsldf 36 19 protocols protocol NNS cord-008590-xivnsldf 36 20 first first RB cord-008590-xivnsldf 36 21 used use VBN cord-008590-xivnsldf 36 22 . . . cord-008590-xivnsldf 37 1 Comparison comparison NN cord-008590-xivnsldf 37 2 of of IN cord-008590-xivnsldf 37 3 data datum NNS cord-008590-xivnsldf 37 4 is be VBZ cord-008590-xivnsldf 37 5 therefore therefore RB cord-008590-xivnsldf 37 6 often often RB cord-008590-xivnsldf 37 7 difficult difficult JJ cord-008590-xivnsldf 37 8 . . . cord-008590-xivnsldf 38 1 The the DT cord-008590-xivnsldf 38 2 extent extent NN cord-008590-xivnsldf 38 3 to to TO cord-008590-xivnsldf 38 4 which which WDT cord-008590-xivnsldf 38 5 ICT ICT NNP cord-008590-xivnsldf 38 6 is be VBZ cord-008590-xivnsldf 38 7 perturbed perturb VBN cord-008590-xivnsldf 38 8 by by IN cord-008590-xivnsldf 38 9 monensin monensin NNP cord-008590-xivnsldf 38 10 is be VBZ cord-008590-xivnsldf 38 11 thought think VBN cord-008590-xivnsldf 38 12 to to TO cord-008590-xivnsldf 38 13 depend depend VB cord-008590-xivnsldf 38 14 on on IN cord-008590-xivnsldf 38 15 the the DT cord-008590-xivnsldf 38 16 alterations alteration NNS cord-008590-xivnsldf 38 17 of of IN cord-008590-xivnsldf 38 18 cytoplasmic cytoplasmic JJ cord-008590-xivnsldf 38 19 ion ion NN cord-008590-xivnsldf 38 20 levels level NNS cord-008590-xivnsldf 38 21 which which WDT cord-008590-xivnsldf 38 22 it -PRON- PRP cord-008590-xivnsldf 38 23 provokes provoke VBZ cord-008590-xivnsldf 38 24 . . . cord-008590-xivnsldf 39 1 The the DT cord-008590-xivnsldf 39 2 impact impact NN cord-008590-xivnsldf 39 3 of of IN cord-008590-xivnsldf 39 4 the the DT cord-008590-xivnsldf 39 5 ionophore ionophore NNP cord-008590-xivnsldf 39 6 may may MD cord-008590-xivnsldf 39 7 therefore therefore RB cord-008590-xivnsldf 39 8 reflect reflect VB cord-008590-xivnsldf 39 9 the the DT cord-008590-xivnsldf 39 10 activity activity NN cord-008590-xivnsldf 39 11 of of IN cord-008590-xivnsldf 39 12 the the DT cord-008590-xivnsldf 39 13 Na Na NNP cord-008590-xivnsldf 39 14 - - HYPH cord-008590-xivnsldf 39 15 K K NNP cord-008590-xivnsldf 39 16 - - HYPH cord-008590-xivnsldf 39 17 ATPase ATPase NNP cord-008590-xivnsldf 39 18 of of IN cord-008590-xivnsldf 39 19 the the DT cord-008590-xivnsldf 39 20 cell cell NN cord-008590-xivnsldf 39 21 under under IN cord-008590-xivnsldf 39 22 study study NN cord-008590-xivnsldf 39 23 . . . cord-008590-xivnsldf 40 1 ably ably RB cord-008590-xivnsldf 40 2 signaling signal VBG cord-008590-xivnsldf 40 3 the the DT cord-008590-xivnsldf 40 4 presence presence NN cord-008590-xivnsldf 40 5 of of IN cord-008590-xivnsldf 40 6 and and CC cord-008590-xivnsldf 40 7 adhering adhere VBG cord-008590-xivnsldf 40 8 to to IN cord-008590-xivnsldf 40 9 an an DT cord-008590-xivnsldf 40 10 overaccumulation overaccumulation NN cord-008590-xivnsldf 40 11 of of IN cord-008590-xivnsldf 40 12 the the DT cord-008590-xivnsldf 40 13 viral viral JJ cord-008590-xivnsldf 40 14 glycoproteins glycoprotein NNS cord-008590-xivnsldf 40 15 . . . cord-008590-xivnsldf 41 1 Since since IN cord-008590-xivnsldf 41 2 other other JJ cord-008590-xivnsldf 41 3 nearby nearby JJ cord-008590-xivnsldf 41 4 Golgi golgi NN cord-008590-xivnsldf 41 5 - - HYPH cord-008590-xivnsldf 41 6 derived derive VBN cord-008590-xivnsldf 41 7 elements element NNS cord-008590-xivnsldf 41 8 are be VBP cord-008590-xivnsldf 41 9 histochemically histochemically RB cord-008590-xivnsldf 41 10 positive positive JJ cord-008590-xivnsldf 41 11 for for IN cord-008590-xivnsldf 41 12 thiamine thiamine NN cord-008590-xivnsldf 41 13 pyrophosphatase pyrophosphatase NN cord-008590-xivnsldf 41 14 ( ( -LRB- cord-008590-xivnsldf 41 15 a a DT cord-008590-xivnsldf 41 16 histochemical histochemical JJ cord-008590-xivnsldf 41 17 marker marker NN cord-008590-xivnsldf 41 18 of of IN cord-008590-xivnsldf 41 19 distal distal JJ cord-008590-xivnsldf 41 20 cisternae cisternae NNP cord-008590-xivnsldf 41 21 ) ) -RRB- cord-008590-xivnsldf 41 22 but but CC cord-008590-xivnsldf 41 23 ICBMs icbm NNS cord-008590-xivnsldf 41 24 are be VBP cord-008590-xivnsldf 41 25 negative negative JJ cord-008590-xivnsldf 41 26 one one NN cord-008590-xivnsldf 41 27 can can MD cord-008590-xivnsldf 41 28 conclude conclude VB cord-008590-xivnsldf 41 29 that that IN cord-008590-xivnsldf 41 30 the the DT cord-008590-xivnsldf 41 31 viral viral JJ cord-008590-xivnsldf 41 32 glycoprotein glycoprotein NN cord-008590-xivnsldf 41 33 has have VBZ cord-008590-xivnsldf 41 34 only only RB cord-008590-xivnsldf 41 35 partly partly RB cord-008590-xivnsldf 41 36 traversed traverse VBN cord-008590-xivnsldf 41 37 the the DT cord-008590-xivnsldf 41 38 Golgi Golgi NNP cord-008590-xivnsldf 41 39 stack stack NN cord-008590-xivnsldf 41 40 . . . cord-008590-xivnsldf 42 1 ( ( -LRB- cord-008590-xivnsldf 42 2 The the DT cord-008590-xivnsldf 42 3 author author NN cord-008590-xivnsldf 42 4 's 's POS cord-008590-xivnsldf 42 5 interpretation interpretation NN cord-008590-xivnsldf 42 6 of of IN cord-008590-xivnsldf 42 7 these these DT cord-008590-xivnsldf 42 8 data datum NNS cord-008590-xivnsldf 42 9 is be VBZ cord-008590-xivnsldf 42 10 in in IN cord-008590-xivnsldf 42 11 fact fact NN cord-008590-xivnsldf 42 12 that that IN cord-008590-xivnsldf 42 13 the the DT cord-008590-xivnsldf 42 14 site site NN cord-008590-xivnsldf 42 15 of of IN cord-008590-xivnsldf 42 16 monensin monensin JJ cord-008590-xivnsldf 42 17 arrest arrest NN cord-008590-xivnsldf 42 18 is be VBZ cord-008590-xivnsldf 42 19 within within IN cord-008590-xivnsldf 42 20 " " `` cord-008590-xivnsldf 42 21 medial medial JJ cord-008590-xivnsldf 42 22 " " '' cord-008590-xivnsldf 42 23 or or CC cord-008590-xivnsldf 42 24 " " `` cord-008590-xivnsldf 42 25 intermediary intermediary JJ cord-008590-xivnsldf 42 26 " " '' cord-008590-xivnsldf 42 27 cisternae cisternae NN cord-008590-xivnsldf 42 28 since since IN cord-008590-xivnsldf 42 29 the the DT cord-008590-xivnsldf 42 30 ICBMs icbms ADD cord-008590-xivnsldf 42 31 bear bear VBP cord-008590-xivnsldf 42 32 ricin ricin NN cord-008590-xivnsldf 42 33 binding binding NN cord-008590-xivnsldf 42 34 sites site NNS cord-008590-xivnsldf 42 35 . . . cord-008590-xivnsldf 42 36 ) ) -RRB- cord-008590-xivnsldf 43 1 Both both CC cord-008590-xivnsldf 43 2 the the DT cord-008590-xivnsldf 43 3 nucleocapsid nucleocapsid NN cord-008590-xivnsldf 43 4 - - HYPH cord-008590-xivnsldf 43 5 laden laden JJ cord-008590-xivnsldf 43 6 ICBMs icbm NNS cord-008590-xivnsldf 43 7 and and CC cord-008590-xivnsldf 43 8 the the DT cord-008590-xivnsldf 43 9 nucleocapsid nucleocapsid NN cord-008590-xivnsldf 43 10 - - HYPH cord-008590-xivnsldf 43 11 free free JJ cord-008590-xivnsldf 43 12 vacuoles vacuole NNS cord-008590-xivnsldf 43 13 can can MD cord-008590-xivnsldf 43 14 be be VB cord-008590-xivnsldf 43 15 partially partially RB cord-008590-xivnsldf 43 16 separated separate VBN cord-008590-xivnsldf 43 17 from from IN cord-008590-xivnsldf 43 18 each each DT cord-008590-xivnsldf 43 19 other other JJ cord-008590-xivnsldf 43 20 ( ( -LRB- cord-008590-xivnsldf 43 21 101 101 CD cord-008590-xivnsldf 43 22 ) ) -RRB- cord-008590-xivnsldf 43 23 ( ( -LRB- cord-008590-xivnsldf 43 24 vide vide NN cord-008590-xivnsldf 43 25 infra infra NN cord-008590-xivnsldf 43 26 ) ) -RRB- cord-008590-xivnsldf 43 27 . . . cord-008590-xivnsldf 44 1 The the DT cord-008590-xivnsldf 44 2 use use NN cord-008590-xivnsldf 44 3 of of IN cord-008590-xivnsldf 44 4 colchicine colchicine NN cord-008590-xivnsldf 44 5 is be VBZ cord-008590-xivnsldf 44 6 more more RBR cord-008590-xivnsldf 44 7 limited limited JJ cord-008590-xivnsldf 44 8 than than IN cord-008590-xivnsldf 44 9 that that DT cord-008590-xivnsldf 44 10 of of IN cord-008590-xivnsldf 44 11 monensin monensin NNP cord-008590-xivnsldf 44 12 , , , cord-008590-xivnsldf 44 13 since since IN cord-008590-xivnsldf 44 14 in in IN cord-008590-xivnsldf 44 15 a a DT cord-008590-xivnsldf 44 16 number number NN cord-008590-xivnsldf 44 17 of of IN cord-008590-xivnsldf 44 18 cell cell NN cord-008590-xivnsldf 44 19 types type NNS cord-008590-xivnsldf 44 20 ICT ICT NNP cord-008590-xivnsldf 44 21 proceeds proceed NNS cord-008590-xivnsldf 44 22 in in IN cord-008590-xivnsldf 44 23 the the DT cord-008590-xivnsldf 44 24 presence presence NN cord-008590-xivnsldf 44 25 of of IN cord-008590-xivnsldf 44 26 the the DT cord-008590-xivnsldf 44 27 drug drug NN cord-008590-xivnsldf 44 28 . . . cord-008590-xivnsldf 45 1 Studies study NNS cord-008590-xivnsldf 45 2 of of IN cord-008590-xivnsldf 45 3 the the DT cord-008590-xivnsldf 45 4 liver liver NN cord-008590-xivnsldf 45 5 suggest suggest VBP cord-008590-xivnsldf 45 6 that that IN cord-008590-xivnsldf 45 7 the the DT cord-008590-xivnsldf 45 8 site site NN cord-008590-xivnsldf 45 9 of of IN cord-008590-xivnsldf 45 10 arrest arrest NN cord-008590-xivnsldf 45 11 of of IN cord-008590-xivnsldf 45 12 ICT ICT NNP cord-008590-xivnsldf 45 13 by by IN cord-008590-xivnsldf 45 14 colchicine colchicine NNP cord-008590-xivnsldf 45 15 may may MD cord-008590-xivnsldf 45 16 be be VB cord-008590-xivnsldf 45 17 later later RB cord-008590-xivnsldf 45 18 than than IN cord-008590-xivnsldf 45 19 the the DT cord-008590-xivnsldf 45 20 monensin monensin NNP cord-008590-xivnsldf 45 21 site site NN cord-008590-xivnsldf 45 22 ( ( -LRB- cord-008590-xivnsldf 45 23 103 103 CD cord-008590-xivnsldf 45 24 , , , cord-008590-xivnsldf 45 25 134 134 CD cord-008590-xivnsldf 45 26 ) ) -RRB- cord-008590-xivnsldf 45 27 . . . cord-008590-xivnsldf 46 1 2 2 LS cord-008590-xivnsldf 46 2 . . . cord-008590-xivnsldf 47 1 The the DT cord-008590-xivnsldf 47 2 approach approach NN cord-008590-xivnsldf 47 3 of of IN cord-008590-xivnsldf 47 4 analyric analyric NNP cord-008590-xivnsldf 47 5 subcellular subcellular JJ cord-008590-xivnsldf 47 6 fractionation fractionation NN cord-008590-xivnsldf 47 7 ( ( -LRB- cord-008590-xivnsldf 47 8 20 20 CD cord-008590-xivnsldf 47 9 , , , cord-008590-xivnsldf 47 10 34a 34a NNS cord-008590-xivnsldf 47 11 , , , cord-008590-xivnsldf 47 12 100 100 CD cord-008590-xivnsldf 47 13 , , , cord-008590-xivnsldf 47 14 101 101 CD cord-008590-xivnsldf 47 15 , , , cord-008590-xivnsldf 47 16 135 135 CD cord-008590-xivnsldf 47 17 , , , cord-008590-xivnsldf 47 18 147 147 CD cord-008590-xivnsldf 47 19 ) ) -RRB- cord-008590-xivnsldf 47 20 in in IN cord-008590-xivnsldf 47 21 which which WDT cord-008590-xivnsldf 47 22 microsomal microsomal NNP cord-008590-xivnsldf 47 23 fractions fraction NNS cord-008590-xivnsldf 47 24 or or CC cord-008590-xivnsldf 47 25 subfractions subfraction NNS cord-008590-xivnsldf 47 26 are be VBP cord-008590-xivnsldf 47 27 spread spread VBN cord-008590-xivnsldf 47 28 , , , cord-008590-xivnsldf 47 29 for for IN cord-008590-xivnsldf 47 30 example example NN cord-008590-xivnsldf 47 31 , , , cord-008590-xivnsldf 47 32 on on IN cord-008590-xivnsldf 47 33 continuous continuous JJ cord-008590-xivnsldf 47 34 density density NN cord-008590-xivnsldf 47 35 gradients gradient NNS cord-008590-xivnsldf 47 36 . . . cord-008590-xivnsldf 48 1 The the DT cord-008590-xivnsldf 48 2 goal goal NN cord-008590-xivnsldf 48 3 of of IN cord-008590-xivnsldf 48 4 this this DT cord-008590-xivnsldf 48 5 method method NN cord-008590-xivnsldf 48 6 is be VBZ cord-008590-xivnsldf 48 7 to to TO cord-008590-xivnsldf 48 8 be be VB cord-008590-xivnsldf 48 9 all all RB cord-008590-xivnsldf 48 10 inclusive inclusive JJ cord-008590-xivnsldf 48 11 rather rather RB cord-008590-xivnsldf 48 12 than than IN cord-008590-xivnsldf 48 13 to to TO cord-008590-xivnsldf 48 14 enrich enrich VB cord-008590-xivnsldf 48 15 for for IN cord-008590-xivnsldf 48 16 selected select VBN cord-008590-xivnsldf 48 17 markers marker NNS cord-008590-xivnsldf 48 18 which which WDT cord-008590-xivnsldf 48 19 might may MD cord-008590-xivnsldf 48 20 prove prove VB cord-008590-xivnsldf 48 21 nonrepresentative nonrepresentative JJ cord-008590-xivnsldf 48 22 of of IN cord-008590-xivnsldf 48 23 the the DT cord-008590-xivnsldf 48 24 whole whole NN cord-008590-xivnsldf 48 25 . . . cord-008590-xivnsldf 49 1 At at IN cord-008590-xivnsldf 49 2 present present JJ cord-008590-xivnsldf 49 3 the the DT cord-008590-xivnsldf 49 4 cytologic cytologic NNP cord-008590-xivnsldf 49 5 origin(s origin(s NNP cord-008590-xivnsldf 49 6 ) ) -RRB- cord-008590-xivnsldf 49 7 of of IN cord-008590-xivnsldf 49 8 such such JJ cord-008590-xivnsldf 49 9 subfractions subfraction NNS cord-008590-xivnsldf 49 10 can can MD cord-008590-xivnsldf 49 11 not not RB cord-008590-xivnsldf 49 12 be be VB cord-008590-xivnsldf 49 13 systematically systematically RB cord-008590-xivnsldf 49 14 identified identify VBN cord-008590-xivnsldf 49 15 . . . cord-008590-xivnsldf 50 1 4 4 CD cord-008590-xivnsldf 50 2 3 3 CD cord-008590-xivnsldf 50 3 . . . cord-008590-xivnsldf 51 1 The the DT cord-008590-xivnsldf 51 2 in in IN cord-008590-xivnsldf 51 3 virro virro NNP cord-008590-xivnsldf 51 4 reconsrirurion reconsrirurion NNP cord-008590-xivnsldf 51 5 approach approach NN cord-008590-xivnsldf 51 6 ( ( -LRB- cord-008590-xivnsldf 51 7 108 108 CD cord-008590-xivnsldf 51 8 , , , cord-008590-xivnsldf 51 9 112 112 CD cord-008590-xivnsldf 51 10 ) ) -RRB- cord-008590-xivnsldf 51 11 in in IN cord-008590-xivnsldf 51 12 which which WDT cord-008590-xivnsldf 51 13 an an DT cord-008590-xivnsldf 51 14 attempt attempt NN cord-008590-xivnsldf 51 15 is be VBZ cord-008590-xivnsldf 51 16 made make VBN cord-008590-xivnsldf 51 17 to to TO cord-008590-xivnsldf 51 18 simulate simulate VB cord-008590-xivnsldf 51 19 selected select VBN cord-008590-xivnsldf 51 20 steps step NNS cord-008590-xivnsldf 51 21 of of IN cord-008590-xivnsldf 51 22 transport transport NN cord-008590-xivnsldf 51 23 by by IN cord-008590-xivnsldf 51 24 mixing mix VBG cord-008590-xivnsldf 51 25 appropriate appropriate JJ cord-008590-xivnsldf 51 26 subcellular subcellular JJ cord-008590-xivnsldf 51 27 fractions fraction NNS cord-008590-xivnsldf 51 28 or or CC cord-008590-xivnsldf 51 29 cell cell NN cord-008590-xivnsldf 51 30 extracts extract NNS cord-008590-xivnsldf 51 31 . . . cord-008590-xivnsldf 52 1 The the DT cord-008590-xivnsldf 52 2 read read NN cord-008590-xivnsldf 52 3 - - HYPH cord-008590-xivnsldf 52 4 out out NN cord-008590-xivnsldf 52 5 from from IN cord-008590-xivnsldf 52 6 such such JJ cord-008590-xivnsldf 52 7 experiments experiment NNS cord-008590-xivnsldf 52 8 may may MD cord-008590-xivnsldf 52 9 involve involve VB cord-008590-xivnsldf 52 10 the the DT cord-008590-xivnsldf 52 11 monitoring monitoring NN cord-008590-xivnsldf 52 12 of of IN cord-008590-xivnsldf 52 13 posttranslational posttranslational JJ cord-008590-xivnsldf 52 14 modifications modification NNS cord-008590-xivnsldf 52 15 which which WDT cord-008590-xivnsldf 52 16 are be VBP cord-008590-xivnsldf 52 17 considered consider VBN cord-008590-xivnsldf 52 18 to to TO cord-008590-xivnsldf 52 19 be be VB cord-008590-xivnsldf 52 20 diagnostic diagnostic JJ cord-008590-xivnsldf 52 21 of of IN cord-008590-xivnsldf 52 22 transport transport NN cord-008590-xivnsldf 52 23 . . . cord-008590-xivnsldf 53 1 This this DT cord-008590-xivnsldf 53 2 is be VBZ cord-008590-xivnsldf 53 3 a a DT cord-008590-xivnsldf 53 4 method method NN cord-008590-xivnsldf 53 5 which which WDT cord-008590-xivnsldf 53 6 may may MD cord-008590-xivnsldf 53 7 provide provide VB cord-008590-xivnsldf 53 8 proof proof NN cord-008590-xivnsldf 53 9 of of IN cord-008590-xivnsldf 53 10 the the DT cord-008590-xivnsldf 53 11 adequacy adequacy NN cord-008590-xivnsldf 53 12 of of IN cord-008590-xivnsldf 53 13 subfractionation subfractionation NN cord-008590-xivnsldf 53 14 procedures procedure NNS cord-008590-xivnsldf 53 15 and and CC cord-008590-xivnsldf 53 16 lead lead VBP cord-008590-xivnsldf 53 17 toward toward IN cord-008590-xivnsldf 53 18 elucidation elucidation NN cord-008590-xivnsldf 53 19 of of IN cord-008590-xivnsldf 53 20 the the DT cord-008590-xivnsldf 53 21 mechanisms mechanism NNS cord-008590-xivnsldf 53 22 of of IN cord-008590-xivnsldf 53 23 transport transport NN cord-008590-xivnsldf 53 24 . . . cord-008590-xivnsldf 54 1 4 4 LS cord-008590-xivnsldf 54 2 . . . cord-008590-xivnsldf 55 1 The the DT cord-008590-xivnsldf 55 2 approach approach NN cord-008590-xivnsldf 55 3 of of IN cord-008590-xivnsldf 55 4 cyrochernisrry cyrochernisrry NNP cord-008590-xivnsldf 55 5 ( ( -LRB- cord-008590-xivnsldf 55 6 36 36 CD cord-008590-xivnsldf 55 7 , , , cord-008590-xivnsldf 55 8 109 109 CD cord-008590-xivnsldf 55 9 , , , cord-008590-xivnsldf 55 10 110 110 CD cord-008590-xivnsldf 55 11 , , , cord-008590-xivnsldf 55 12 138 138 CD cord-008590-xivnsldf 55 13 ) ) -RRB- cord-008590-xivnsldf 55 14 making make VBG cord-008590-xivnsldf 55 15 use use NN cord-008590-xivnsldf 55 16 of of IN cord-008590-xivnsldf 55 17 antisera antisera NN cord-008590-xivnsldf 55 18 which which WDT cord-008590-xivnsldf 55 19 recognize recognize VBP cord-008590-xivnsldf 55 20 processing processing NN cord-008590-xivnsldf 55 21 enzymes enzyme NNS cord-008590-xivnsldf 55 22 or or CC cord-008590-xivnsldf 55 23 lectin lectin NN cord-008590-xivnsldf 55 24 conjugates conjugate NNS cord-008590-xivnsldf 55 25 . . . cord-008590-xivnsldf 56 1 This this DT cord-008590-xivnsldf 56 2 method method NN cord-008590-xivnsldf 56 3 can can MD cord-008590-xivnsldf 56 4 not not RB cord-008590-xivnsldf 56 5 indicate indicate VB cord-008590-xivnsldf 56 6 at at IN cord-008590-xivnsldf 56 7 which which WDT cord-008590-xivnsldf 56 8 site site NN cord-008590-xivnsldf 56 9 processing processing NN cord-008590-xivnsldf 56 10 enzymes enzyme NNS cord-008590-xivnsldf 56 11 are be VBP cord-008590-xivnsldf 56 12 active active JJ cord-008590-xivnsldf 56 13 , , , cord-008590-xivnsldf 56 14 but but CC cord-008590-xivnsldf 56 15 it -PRON- PRP cord-008590-xivnsldf 56 16 should should MD cord-008590-xivnsldf 56 17 locate locate VB cord-008590-xivnsldf 56 18 the the DT cord-008590-xivnsldf 56 19 enzymes enzyme NNS cord-008590-xivnsldf 56 20 in in IN cord-008590-xivnsldf 56 21 a a DT cord-008590-xivnsldf 56 22 cytologic cytologic JJ cord-008590-xivnsldf 56 23 context context NN cord-008590-xivnsldf 56 24 . . . cord-008590-xivnsldf 57 1 The the DT cord-008590-xivnsldf 57 2 lectin lectin NN cord-008590-xivnsldf 57 3 conjugates conjugate NNS cord-008590-xivnsldf 57 4 localize localize VBP cord-008590-xivnsldf 57 5 products product NNS cord-008590-xivnsldf 57 6 of of IN cord-008590-xivnsldf 57 7 sugar sugar NN cord-008590-xivnsldf 57 8 transferase transferase NN cord-008590-xivnsldf 57 9 activity activity NN cord-008590-xivnsldf 57 10 , , , cord-008590-xivnsldf 57 11 with with IN cord-008590-xivnsldf 57 12 the the DT cord-008590-xivnsldf 57 13 reservation reservation NN cord-008590-xivnsldf 57 14 that that IN cord-008590-xivnsldf 57 15 it -PRON- PRP cord-008590-xivnsldf 57 16 may may MD cord-008590-xivnsldf 57 17 prove prove VB cord-008590-xivnsldf 57 18 difficult difficult JJ cord-008590-xivnsldf 57 19 to to TO cord-008590-xivnsldf 57 20 establish establish VB cord-008590-xivnsldf 57 21 whether whether IN cord-008590-xivnsldf 57 22 a a DT cord-008590-xivnsldf 57 23 given give VBN cord-008590-xivnsldf 57 24 sugar sugar NN cord-008590-xivnsldf 57 25 residue residue NN cord-008590-xivnsldf 57 26 is be VBZ cord-008590-xivnsldf 57 27 borne bear VBN cord-008590-xivnsldf 57 28 by by IN cord-008590-xivnsldf 57 29 an an DT cord-008590-xivnsldf 57 30 N n CD cord-008590-xivnsldf 57 31 - - HYPH cord-008590-xivnsldf 57 32 or or CC cord-008590-xivnsldf 57 33 0-linked 0-linked CD cord-008590-xivnsldf 57 34 or or CC cord-008590-xivnsldf 57 35 possibly possibly RB cord-008590-xivnsldf 57 36 lipid lipid NN cord-008590-xivnsldf 57 37 carrier carrier NN cord-008590-xivnsldf 57 38 . . . cord-008590-xivnsldf 58 1 4The 4the CD cord-008590-xivnsldf 58 2 presence presence NN cord-008590-xivnsldf 58 3 of of IN cord-008590-xivnsldf 58 4 lipoprotein lipoprotein NN cord-008590-xivnsldf 58 5 particles particle NNS cord-008590-xivnsldf 58 6 within within IN cord-008590-xivnsldf 58 7 the the DT cord-008590-xivnsldf 58 8 Golgi Golgi NNP cord-008590-xivnsldf 58 9 cisternae cisternae NN cord-008590-xivnsldf 58 10 of of IN cord-008590-xivnsldf 58 11 the the DT cord-008590-xivnsldf 58 12 hepatocyte hepatocyte NN cord-008590-xivnsldf 58 13 has have VBZ cord-008590-xivnsldf 58 14 major major JJ cord-008590-xivnsldf 58 15 implications implication NNS cord-008590-xivnsldf 58 16 for for IN cord-008590-xivnsldf 58 17 liver liver NN cord-008590-xivnsldf 58 18 Golgi Golgi NNP cord-008590-xivnsldf 58 19 subfractionation subfractionation NN cord-008590-xivnsldf 58 20 . . . cord-008590-xivnsldf 59 1 Preparative Preparative NNP cord-008590-xivnsldf 59 2 Golgi Golgi NNP cord-008590-xivnsldf 59 3 fractions fraction NNS cord-008590-xivnsldf 59 4 can can MD cord-008590-xivnsldf 59 5 be be VB cord-008590-xivnsldf 59 6 obtained obtain VBN cord-008590-xivnsldf 59 7 free free JJ cord-008590-xivnsldf 59 8 from from IN cord-008590-xivnsldf 59 9 smooth smooth JJ cord-008590-xivnsldf 59 10 ER er NN cord-008590-xivnsldf 59 11 and and CC cord-008590-xivnsldf 59 12 plasma plasma NN cord-008590-xivnsldf 59 13 membrane membrane NN cord-008590-xivnsldf 59 14 because because IN cord-008590-xivnsldf 59 15 of of IN cord-008590-xivnsldf 59 16 the the DT cord-008590-xivnsldf 59 17 density density NN cord-008590-xivnsldf 59 18 perturbation perturbation NN cord-008590-xivnsldf 59 19 which which WDT cord-008590-xivnsldf 59 20 results result VBZ cord-008590-xivnsldf 59 21 from from IN cord-008590-xivnsldf 59 22 this this DT cord-008590-xivnsldf 59 23 buoyant buoyant JJ cord-008590-xivnsldf 59 24 content content NN cord-008590-xivnsldf 59 25 . . . cord-008590-xivnsldf 60 1 For for IN cord-008590-xivnsldf 60 2 analytic analytic JJ cord-008590-xivnsldf 60 3 isopycnic isopycnic NNP cord-008590-xivnsldf 60 4 fractionation fractionation NN cord-008590-xivnsldf 60 5 one one PRP cord-008590-xivnsldf 60 6 would would MD cord-008590-xivnsldf 60 7 anticipate anticipate VB cord-008590-xivnsldf 60 8 a a DT cord-008590-xivnsldf 60 9 dispersion dispersion NN cord-008590-xivnsldf 60 10 of of IN cord-008590-xivnsldf 60 11 density density NN cord-008590-xivnsldf 60 12 in in IN cord-008590-xivnsldf 60 13 proportion proportion NN cord-008590-xivnsldf 60 14 to to IN cord-008590-xivnsldf 60 15 variability variability NN cord-008590-xivnsldf 60 16 in in IN cord-008590-xivnsldf 60 17 the the DT cord-008590-xivnsldf 60 18 number number NN cord-008590-xivnsldf 60 19 of of IN cord-008590-xivnsldf 60 20 lipoprotein lipoprotein NN cord-008590-xivnsldf 60 21 particles particle NNS cord-008590-xivnsldf 60 22 contained contain VBN cord-008590-xivnsldf 60 23 within within IN cord-008590-xivnsldf 60 24 Golgi Golgi NNP cord-008590-xivnsldf 60 25 fragments fragment NNS cord-008590-xivnsldf 60 26 produced produce VBN cord-008590-xivnsldf 60 27 by by IN cord-008590-xivnsldf 60 28 homogenization homogenization NN cord-008590-xivnsldf 60 29 . . . cord-008590-xivnsldf 61 1 Moreover moreover RB cord-008590-xivnsldf 61 2 , , , cord-008590-xivnsldf 61 3 the the DT cord-008590-xivnsldf 61 4 relative relative JJ cord-008590-xivnsldf 61 5 isopycnic isopycnic JJ cord-008590-xivnsldf 61 6 density density NN cord-008590-xivnsldf 61 7 of of IN cord-008590-xivnsldf 61 8 different different JJ cord-008590-xivnsldf 61 9 subregions subregion NNS cord-008590-xivnsldf 61 10 of of IN cord-008590-xivnsldf 61 11 the the DT cord-008590-xivnsldf 61 12 GC GC NNP cord-008590-xivnsldf 61 13 would would MD cord-008590-xivnsldf 61 14 reflect reflect VB cord-008590-xivnsldf 61 15 the the DT cord-008590-xivnsldf 61 16 degree degree NN cord-008590-xivnsldf 61 17 of of IN cord-008590-xivnsldf 61 18 maturation maturation NN cord-008590-xivnsldf 61 19 of of IN cord-008590-xivnsldf 61 20 the the DT cord-008590-xivnsldf 61 21 lipoproteins lipoprotein NNS cord-008590-xivnsldf 61 22 . . . cord-008590-xivnsldf 62 1 If if IN cord-008590-xivnsldf 62 2 the the DT cord-008590-xivnsldf 62 3 lipid lipid NN cord-008590-xivnsldf 62 4 content content NN cord-008590-xivnsldf 62 5 - - : cord-008590-xivnsldf 62 6 and and CC cord-008590-xivnsldf 62 7 as as IN cord-008590-xivnsldf 62 8 a a DT cord-008590-xivnsldf 62 9 result result NN cord-008590-xivnsldf 62 10 the the DT cord-008590-xivnsldf 62 11 density density NN cord-008590-xivnsldf 62 12 shift shift NN cord-008590-xivnsldf 62 13 - - NNS cord-008590-xivnsldf 62 14 increases increase NNS cord-008590-xivnsldf 62 15 monotonically monotonically RB cord-008590-xivnsldf 62 16 during during IN cord-008590-xivnsldf 62 17 ICT ICT NNP cord-008590-xivnsldf 62 18 then then RB cord-008590-xivnsldf 62 19 the the DT cord-008590-xivnsldf 62 20 relative relative JJ cord-008590-xivnsldf 62 21 densities density NNS cord-008590-xivnsldf 62 22 of of IN cord-008590-xivnsldf 62 23 Golgi Golgi NNP cord-008590-xivnsldf 62 24 subfractions subfraction NNS cord-008590-xivnsldf 62 25 might may MD cord-008590-xivnsldf 62 26 correspond correspond VB cord-008590-xivnsldf 62 27 inversely inversely RB cord-008590-xivnsldf 62 28 to to IN cord-008590-xivnsldf 62 29 their -PRON- PRP$ cord-008590-xivnsldf 62 30 position position NN cord-008590-xivnsldf 62 31 along along IN cord-008590-xivnsldf 62 32 the the DT cord-008590-xivnsldf 62 33 time time NN cord-008590-xivnsldf 62 34 axis axis NN cord-008590-xivnsldf 62 35 of of IN cord-008590-xivnsldf 62 36 transport transport NN cord-008590-xivnsldf 62 37 ; ; : cord-008590-xivnsldf 62 38 however however RB cord-008590-xivnsldf 62 39 , , , cord-008590-xivnsldf 62 40 there there EX cord-008590-xivnsldf 62 41 is be VBZ cord-008590-xivnsldf 62 42 no no DT cord-008590-xivnsldf 62 43 proof proof NN cord-008590-xivnsldf 62 44 that that IN cord-008590-xivnsldf 62 45 such such PDT cord-008590-xivnsldf 62 46 a a DT cord-008590-xivnsldf 62 47 monotonic monotonic JJ cord-008590-xivnsldf 62 48 relation relation NN cord-008590-xivnsldf 62 49 applies apply VBZ cord-008590-xivnsldf 62 50 . . . cord-008590-xivnsldf 63 1 CONFINED CONFINED NNP cord-008590-xivnsldf 63 2 FUNCTION function NN cord-008590-xivnsldf 63 3 MODEL model NN cord-008590-xivnsldf 63 4 OF of IN cord-008590-xivnsldf 63 5 THE the DT cord-008590-xivnsldf 63 6 GOLGI GOLGI NNP cord-008590-xivnsldf 63 7 COMPLEX COMPLEX NNP cord-008590-xivnsldf 63 8 225 225 CD cord-008590-xivnsldf 63 9 5 5 CD cord-008590-xivnsldf 63 10 . . . cord-008590-xivnsldf 64 1 A a DT cord-008590-xivnsldf 64 2 possible possible JJ cord-008590-xivnsldf 64 3 means mean NNS cord-008590-xivnsldf 64 4 of of IN cord-008590-xivnsldf 64 5 localizing localize VBG cord-008590-xivnsldf 64 6 a a DT cord-008590-xivnsldf 64 7 Golgi Golgi NNP cord-008590-xivnsldf 64 8 component component NN cord-008590-xivnsldf 64 9 relative relative JJ cord-008590-xivnsldf 64 10 to to IN cord-008590-xivnsldf 64 11 selected select VBN cord-008590-xivnsldf 64 12 processing processing NN cord-008590-xivnsldf 64 13 enzymes enzyme NNS cord-008590-xivnsldf 64 14 is be VBZ cord-008590-xivnsldf 64 15 to to TO cord-008590-xivnsldf 64 16 inquire inquire VB cord-008590-xivnsldf 64 17 whether whether IN cord-008590-xivnsldf 64 18 or or CC cord-008590-xivnsldf 64 19 not not RB cord-008590-xivnsldf 64 20 it -PRON- PRP cord-008590-xivnsldf 64 21 has have VBZ cord-008590-xivnsldf 64 22 undergone undergo VBN cord-008590-xivnsldf 64 23 proteolytic proteolytic JJ cord-008590-xivnsldf 64 24 cleavage cleavage NN cord-008590-xivnsldf 64 25 , , , cord-008590-xivnsldf 64 26 terminal terminal JJ cord-008590-xivnsldf 64 27 sugar sugar NN cord-008590-xivnsldf 64 28 addition addition NN cord-008590-xivnsldf 64 29 to to IN cord-008590-xivnsldf 64 30 N n CD cord-008590-xivnsldf 64 31 - - HYPH cord-008590-xivnsldf 64 32 linked link VBN cord-008590-xivnsldf 64 33 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 64 34 , , , cord-008590-xivnsldf 64 35 initiation initiation NN cord-008590-xivnsldf 64 36 or or CC cord-008590-xivnsldf 64 37 completion completion NN cord-008590-xivnsldf 64 38 of of IN cord-008590-xivnsldf 64 39 any any DT cord-008590-xivnsldf 64 40 O o NN cord-008590-xivnsldf 64 41 - - HYPH cord-008590-xivnsldf 64 42 linked link VBN cord-008590-xivnsldf 64 43 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 64 44 etc etc FW cord-008590-xivnsldf 64 45 . . . cord-008590-xivnsldf 65 1 The the DT cord-008590-xivnsldf 65 2 degree degree NN cord-008590-xivnsldf 65 3 to to TO cord-008590-xivnsldf 65 4 which which WDT cord-008590-xivnsldf 65 5 it -PRON- PRP cord-008590-xivnsldf 65 6 has have VBZ cord-008590-xivnsldf 65 7 undergone undergo VBN cord-008590-xivnsldf 65 8 posttranslational posttranslational JJ cord-008590-xivnsldf 65 9 modifications modification NNS cord-008590-xivnsldf 65 10 should should MD cord-008590-xivnsldf 65 11 reflect reflect VB cord-008590-xivnsldf 65 12 its -PRON- PRP$ cord-008590-xivnsldf 65 13 ontogeny ontogeny NN cord-008590-xivnsldf 65 14 and and CC cord-008590-xivnsldf 65 15 site site NN cord-008590-xivnsldf 65 16 of of IN cord-008590-xivnsldf 65 17 residence residence NN cord-008590-xivnsldf 65 18 . . . cord-008590-xivnsldf 66 1 6 6 LS cord-008590-xivnsldf 66 2 . . . cord-008590-xivnsldf 67 1 A a DT cord-008590-xivnsldf 67 2 kinetic kinetic JJ cord-008590-xivnsldf 67 3 ordering ordering NN cord-008590-xivnsldf 67 4 of of IN cord-008590-xivnsldf 67 5 processing processing NN cord-008590-xivnsldf 67 6 events event NNS cord-008590-xivnsldf 67 7 can can MD cord-008590-xivnsldf 67 8 be be VB cord-008590-xivnsldf 67 9 obtained obtain VBN cord-008590-xivnsldf 67 10 by by IN cord-008590-xivnsldf 67 11 performing perform VBG cord-008590-xivnsldf 67 12 , , , cord-008590-xivnsldf 67 13 for for IN cord-008590-xivnsldf 67 14 example example NN cord-008590-xivnsldf 67 15 , , , cord-008590-xivnsldf 67 16 an an DT cord-008590-xivnsldf 67 17 amino amino NN cord-008590-xivnsldf 67 18 acid acid NN cord-008590-xivnsldf 67 19 labeling labeling NN cord-008590-xivnsldf 67 20 pulse pulse NN cord-008590-xivnsldf 67 21 - - HYPH cord-008590-xivnsldf 67 22 chase chase NN cord-008590-xivnsldf 67 23 experiment experiment NN cord-008590-xivnsldf 67 24 and and CC cord-008590-xivnsldf 67 25 inquiring inquire VBG cord-008590-xivnsldf 67 26 as as IN cord-008590-xivnsldf 67 27 a a DT cord-008590-xivnsldf 67 28 function function NN cord-008590-xivnsldf 67 29 of of IN cord-008590-xivnsldf 67 30 time time NN cord-008590-xivnsldf 67 31 , , , cord-008590-xivnsldf 67 32 when when WRB cord-008590-xivnsldf 67 33 the the DT cord-008590-xivnsldf 67 34 product product NN cord-008590-xivnsldf 67 35 in in IN cord-008590-xivnsldf 67 36 question question NN cord-008590-xivnsldf 67 37 undergoes undergo VBZ cord-008590-xivnsldf 67 38 one one CD cord-008590-xivnsldf 67 39 or or CC cord-008590-xivnsldf 67 40 another another DT cord-008590-xivnsldf 67 41 maturation maturation NN cord-008590-xivnsldf 67 42 step step NN cord-008590-xivnsldf 67 43 . . . cord-008590-xivnsldf 68 1 Distinct distinct JJ cord-008590-xivnsldf 68 2 kinetics kinetic NNS cord-008590-xivnsldf 68 3 need need VBP cord-008590-xivnsldf 68 4 not not RB cord-008590-xivnsldf 68 5 , , , cord-008590-xivnsldf 68 6 of of IN cord-008590-xivnsldf 68 7 course course NN cord-008590-xivnsldf 68 8 , , , cord-008590-xivnsldf 68 9 imply imply VBP cord-008590-xivnsldf 68 10 distinct distinct JJ cord-008590-xivnsldf 68 11 compartmentalization compartmentalization NN cord-008590-xivnsldf 68 12 . . . cord-008590-xivnsldf 69 1 The the DT cord-008590-xivnsldf 69 2 resolution resolution NN cord-008590-xivnsldf 69 3 of of IN cord-008590-xivnsldf 69 4 this this DT cord-008590-xivnsldf 69 5 approach approach NN cord-008590-xivnsldf 69 6 is be VBZ cord-008590-xivnsldf 69 7 limited limit VBN cord-008590-xivnsldf 69 8 by by IN cord-008590-xivnsldf 69 9 any any DT cord-008590-xivnsldf 69 10 dispersion dispersion NN cord-008590-xivnsldf 69 11 of of IN cord-008590-xivnsldf 69 12 transport transport NN cord-008590-xivnsldf 69 13 rates rate NNS cord-008590-xivnsldf 69 14 of of IN cord-008590-xivnsldf 69 15 the the DT cord-008590-xivnsldf 69 16 pulse pulse NN cord-008590-xivnsldf 69 17 - - HYPH cord-008590-xivnsldf 69 18 labeled label VBN cord-008590-xivnsldf 69 19 molecules molecule NNS cord-008590-xivnsldf 69 20 . . . cord-008590-xivnsldf 70 1 The the DT cord-008590-xivnsldf 70 2 exploitation exploitation NN cord-008590-xivnsldf 70 3 of of IN cord-008590-xivnsldf 70 4 these these DT cord-008590-xivnsldf 70 5 approaches approach NNS cord-008590-xivnsldf 70 6 has have VBZ cord-008590-xivnsldf 70 7 by by IN cord-008590-xivnsldf 70 8 no no DT cord-008590-xivnsldf 70 9 means means NN cord-008590-xivnsldf 70 10 been be VBN cord-008590-xivnsldf 70 11 sufficiently sufficiently RB cord-008590-xivnsldf 70 12 extensive extensive JJ cord-008590-xivnsldf 70 13 to to TO cord-008590-xivnsldf 70 14 allow allow VB cord-008590-xivnsldf 70 15 assignment assignment NN cord-008590-xivnsldf 70 16 of of IN cord-008590-xivnsldf 70 17 the the DT cord-008590-xivnsldf 70 18 multitude multitude NN cord-008590-xivnsldf 70 19 of of IN cord-008590-xivnsldf 70 20 processing process VBG cord-008590-xivnsldf 70 21 events event NNS cord-008590-xivnsldf 70 22 to to IN cord-008590-xivnsldf 70 23 subregions subregion NNS cord-008590-xivnsldf 70 24 of of IN cord-008590-xivnsldf 70 25 the the DT cord-008590-xivnsldf 70 26 GC GC NNP cord-008590-xivnsldf 70 27 . . . cord-008590-xivnsldf 71 1 Moreover moreover RB cord-008590-xivnsldf 71 2 , , , cord-008590-xivnsldf 71 3 the the DT cord-008590-xivnsldf 71 4 present present JJ cord-008590-xivnsldf 71 5 lack lack NN cord-008590-xivnsldf 71 6 of of IN cord-008590-xivnsldf 71 7 detailed detailed JJ cord-008590-xivnsldf 71 8 knowledge knowledge NN cord-008590-xivnsldf 71 9 of of IN cord-008590-xivnsldf 71 10 the the DT cord-008590-xivnsldf 71 11 GC GC NNP cord-008590-xivnsldf 71 12 is be VBZ cord-008590-xivnsldf 71 13 such such JJ cord-008590-xivnsldf 71 14 that that IN cord-008590-xivnsldf 71 15 it -PRON- PRP cord-008590-xivnsldf 71 16 is be VBZ cord-008590-xivnsldf 71 17 the the DT cord-008590-xivnsldf 71 18 analysis analysis NN cord-008590-xivnsldf 71 19 of of IN cord-008590-xivnsldf 71 20 numerous numerous JJ cord-008590-xivnsldf 71 21 processing processing NN cord-008590-xivnsldf 71 22 events event NNS cord-008590-xivnsldf 71 23 which which WDT cord-008590-xivnsldf 71 24 may may MD cord-008590-xivnsldf 71 25 actually actually RB cord-008590-xivnsldf 71 26 serve serve VB cord-008590-xivnsldf 71 27 to to TO cord-008590-xivnsldf 71 28 enumerate enumerate VB cord-008590-xivnsldf 71 29 the the DT cord-008590-xivnsldf 71 30 number number NN cord-008590-xivnsldf 71 31 of of IN cord-008590-xivnsldf 71 32 subcompartments subcompartment NNS cord-008590-xivnsldf 71 33 which which WDT cord-008590-xivnsldf 71 34 exist exist VBP cord-008590-xivnsldf 71 35 . . . cord-008590-xivnsldf 72 1 Judging judge VBG cord-008590-xivnsldf 72 2 from from IN cord-008590-xivnsldf 72 3 histochemical histochemical JJ cord-008590-xivnsldf 72 4 data data NNP cord-008590-xivnsldf 72 5 ( ( -LRB- cord-008590-xivnsldf 72 6 24 24 CD cord-008590-xivnsldf 72 7 , , , cord-008590-xivnsldf 72 8 35 35 CD cord-008590-xivnsldf 72 9 , , , cord-008590-xivnsldf 72 10 111 111 CD cord-008590-xivnsldf 72 11 , , , cord-008590-xivnsldf 72 12 134 134 CD cord-008590-xivnsldf 72 13 ) ) -RRB- cord-008590-xivnsldf 72 14 , , , cord-008590-xivnsldf 72 15 at at RB cord-008590-xivnsldf 72 16 least least RBS cord-008590-xivnsldf 72 17 three three CD cord-008590-xivnsldf 72 18 classes class NNS cord-008590-xivnsldf 72 19 of of IN cord-008590-xivnsldf 72 20 cisternae cisternae NNP cord-008590-xivnsldf 72 21 can can MD cord-008590-xivnsldf 72 22 be be VB cord-008590-xivnsldf 72 23 distinguished distinguish VBN cord-008590-xivnsldf 72 24 : : : cord-008590-xivnsldf 72 25 the the DT cord-008590-xivnsldf 72 26 most most RBS cord-008590-xivnsldf 72 27 proximal proximal JJ cord-008590-xivnsldf 72 28 ( ( -LRB- cord-008590-xivnsldf 72 29 overstained overstaine VBN cord-008590-xivnsldf 72 30 by by IN cord-008590-xivnsldf 72 31 OsO oso NN cord-008590-xivnsldf 72 32 , , , cord-008590-xivnsldf 72 33 ) ) -RRB- cord-008590-xivnsldf 72 34 , , , cord-008590-xivnsldf 72 35 medial medial NNP cord-008590-xivnsldf 72 36 cisternae cisternae NNP cord-008590-xivnsldf 72 37 ( ( -LRB- cord-008590-xivnsldf 72 38 reactive reactive JJ cord-008590-xivnsldf 72 39 for for IN cord-008590-xivnsldf 72 40 nicotinamide nicotinamide NN cord-008590-xivnsldf 72 41 adenine adenine NN cord-008590-xivnsldf 72 42 dinucleotide dinucleotide NN cord-008590-xivnsldf 72 43 phosphatase phosphatase NN cord-008590-xivnsldf 72 44 at at IN cord-008590-xivnsldf 72 45 pH pH NNP cord-008590-xivnsldf 72 46 5 5 CD cord-008590-xivnsldf 72 47 in in IN cord-008590-xivnsldf 72 48 certain certain JJ cord-008590-xivnsldf 72 49 cells cell NNS cord-008590-xivnsldf 72 50 ) ) -RRB- cord-008590-xivnsldf 72 51 and and CC cord-008590-xivnsldf 72 52 the the DT cord-008590-xivnsldf 72 53 most most RBS cord-008590-xivnsldf 72 54 distal distal JJ cord-008590-xivnsldf 72 55 ( ( -LRB- cord-008590-xivnsldf 72 56 reactive reactive JJ cord-008590-xivnsldf 72 57 for for IN cord-008590-xivnsldf 72 58 nucleoside nucleoside NN cord-008590-xivnsldf 72 59 diphosphatase diphosphatase NN cord-008590-xivnsldf 72 60 = = SYM cord-008590-xivnsldf 72 61 uridine uridine NNP cord-008590-xivnsldf 72 62 diphosphatase diphosphatase NN cord-008590-xivnsldf 72 63 = = SYM cord-008590-xivnsldf 72 64 thiamine thiamine NNP cord-008590-xivnsldf 72 65 pyrophosphatase pyrophosphatase NN cord-008590-xivnsldf 72 66 ) ) -RRB- cord-008590-xivnsldf 72 67 . . . cord-008590-xivnsldf 73 1 In in IN cord-008590-xivnsldf 73 2 addition addition NN cord-008590-xivnsldf 73 3 , , , cord-008590-xivnsldf 73 4 the the DT cord-008590-xivnsldf 73 5 structurally structurally RB cord-008590-xivnsldf 73 6 somewhat somewhat RB cord-008590-xivnsldf 73 7 removed remove VBN cord-008590-xivnsldf 73 8 tubules tubule NNS cord-008590-xivnsldf 73 9 and and CC cord-008590-xivnsldf 73 10 cisternae cisternae NN cord-008590-xivnsldf 73 11 of of IN cord-008590-xivnsldf 73 12 " " `` cord-008590-xivnsldf 73 13 GERL GERL NNP cord-008590-xivnsldf 73 14 " " '' cord-008590-xivnsldf 73 15 are be VBP cord-008590-xivnsldf 73 16 histochemically histochemically RB cord-008590-xivnsldf 73 17 reactive reactive JJ cord-008590-xivnsldf 73 18 for for IN cord-008590-xivnsldf 73 19 acid acid JJ cord-008590-xivnsldf 73 20 phosphatase phosphatase NN cord-008590-xivnsldf 73 21 . . . cord-008590-xivnsldf 74 1 Present present JJ cord-008590-xivnsldf 74 2 studies study NNS cord-008590-xivnsldf 74 3 employing employ VBG cord-008590-xivnsldf 74 4 lectins lectin NNS cord-008590-xivnsldf 74 5 to to TO cord-008590-xivnsldf 74 6 stain stain VB cord-008590-xivnsldf 74 7 the the DT cord-008590-xivnsldf 74 8 Golgi Golgi NNP cord-008590-xivnsldf 74 9 cisternae cisternae NN cord-008590-xivnsldf 74 10 of of IN cord-008590-xivnsldf 74 11 various various JJ cord-008590-xivnsldf 74 12 cells cell NNS cord-008590-xivnsldf 74 13 have have VBP cord-008590-xivnsldf 74 14 also also RB cord-008590-xivnsldf 74 15 distinguished distinguish VBN cord-008590-xivnsldf 74 16 two two CD cord-008590-xivnsldf 74 17 or or CC cord-008590-xivnsldf 74 18 three three CD cord-008590-xivnsldf 74 19 distinct distinct JJ cord-008590-xivnsldf 74 20 staining stain VBG cord-008590-xivnsldf 74 21 regions region NNS cord-008590-xivnsldf 74 22 along along IN cord-008590-xivnsldf 74 23 the the DT cord-008590-xivnsldf 74 24 proximal proximal JJ cord-008590-xivnsldf 74 25 - - HYPH cord-008590-xivnsldf 74 26 to to IN cord-008590-xivnsldf 74 27 - - HYPH cord-008590-xivnsldf 74 28 distal distal JJ cord-008590-xivnsldf 74 29 axis axis NN cord-008590-xivnsldf 74 30 ( ( -LRB- cord-008590-xivnsldf 74 31 36 36 CD cord-008590-xivnsldf 74 32 , , , cord-008590-xivnsldf 74 33 37 37 CD cord-008590-xivnsldf 74 34 , , , cord-008590-xivnsldf 74 35 109 109 CD cord-008590-xivnsldf 74 36 , , , cord-008590-xivnsldf 74 37 138 138 CD cord-008590-xivnsldf 74 38 ) ) -RRB- cord-008590-xivnsldf 75 1 ( ( -LRB- cord-008590-xivnsldf 75 2 Table table NN cord-008590-xivnsldf 75 3 I i CD cord-008590-xivnsldf 75 4 ) ) -RRB- cord-008590-xivnsldf 75 5 . . . cord-008590-xivnsldf 76 1 The the DT cord-008590-xivnsldf 76 2 idea idea NN cord-008590-xivnsldf 76 3 of of IN cord-008590-xivnsldf 76 4 subcompartmentalization subcompartmentalization NN cord-008590-xivnsldf 76 5 of of IN cord-008590-xivnsldf 76 6 the the DT cord-008590-xivnsldf 76 7 GC GC NNP cord-008590-xivnsldf 76 8 is be VBZ cord-008590-xivnsldf 76 9 discussed discuss VBN cord-008590-xivnsldf 76 10 here here RB cord-008590-xivnsldf 76 11 in in IN cord-008590-xivnsldf 76 12 the the DT cord-008590-xivnsldf 76 13 context context NN cord-008590-xivnsldf 76 14 of of IN cord-008590-xivnsldf 76 15 distinct distinct JJ cord-008590-xivnsldf 76 16 varieties variety NNS cord-008590-xivnsldf 76 17 of of IN cord-008590-xivnsldf 76 18 posttranslational posttranslational JJ cord-008590-xivnsldf 76 19 processing processing NN cord-008590-xivnsldf 76 20 several several JJ cord-008590-xivnsldf 76 21 of of IN cord-008590-xivnsldf 76 22 which which WDT cord-008590-xivnsldf 76 23 are be VBP cord-008590-xivnsldf 76 24 illustrated illustrate VBN cord-008590-xivnsldf 76 25 , , , cord-008590-xivnsldf 76 26 in in IN cord-008590-xivnsldf 76 27 abbreviated abbreviate VBN cord-008590-xivnsldf 76 28 form form NN cord-008590-xivnsldf 76 29 , , , cord-008590-xivnsldf 76 30 in in IN cord-008590-xivnsldf 76 31 Figs Figs NNPS cord-008590-xivnsldf 76 32 . . . cord-008590-xivnsldf 77 1 1 1 LS cord-008590-xivnsldf 77 2 - - SYM cord-008590-xivnsldf 77 3 4 4 CD cord-008590-xivnsldf 77 4 . . . cord-008590-xivnsldf 78 1 To to IN cord-008590-xivnsldf 78 2 a a DT cord-008590-xivnsldf 78 3 certain certain JJ cord-008590-xivnsldf 78 4 extent extent NN cord-008590-xivnsldf 78 5 , , , cord-008590-xivnsldf 78 6 these these DT cord-008590-xivnsldf 78 7 events event NNS cord-008590-xivnsldf 78 8 might may MD cord-008590-xivnsldf 78 9 all all DT cord-008590-xivnsldf 78 10 be be VB cord-008590-xivnsldf 78 11 found find VBN cord-008590-xivnsldf 78 12 within within IN cord-008590-xivnsldf 78 13 a a DT cord-008590-xivnsldf 78 14 single single JJ cord-008590-xivnsldf 78 15 cell cell NN cord-008590-xivnsldf 78 16 ; ; : cord-008590-xivnsldf 78 17 however however RB cord-008590-xivnsldf 78 18 , , , cord-008590-xivnsldf 78 19 since since IN cord-008590-xivnsldf 78 20 the the DT cord-008590-xivnsldf 78 21 biosynthetic biosynthetic JJ cord-008590-xivnsldf 78 22 repertoire repertoire NN cord-008590-xivnsldf 78 23 ( ( -LRB- cord-008590-xivnsldf 78 24 e.g. e.g. RB cord-008590-xivnsldf 78 25 , , , cord-008590-xivnsldf 78 26 of of IN cord-008590-xivnsldf 78 27 exported export VBN cord-008590-xivnsldf 78 28 glycoconjugates glycoconjugate NNS cord-008590-xivnsldf 78 29 ) ) -RRB- cord-008590-xivnsldf 78 30 varies vary VBZ cord-008590-xivnsldf 78 31 among among IN cord-008590-xivnsldf 78 32 cell cell NN cord-008590-xivnsldf 78 33 types type NNS cord-008590-xivnsldf 78 34 , , , cord-008590-xivnsldf 78 35 there there EX cord-008590-xivnsldf 78 36 is be VBZ cord-008590-xivnsldf 78 37 every every DT cord-008590-xivnsldf 78 38 reason reason NN cord-008590-xivnsldf 78 39 to to TO cord-008590-xivnsldf 78 40 anticipate anticipate VB cord-008590-xivnsldf 78 41 significant significant JJ cord-008590-xivnsldf 78 42 enzymologic enzymologic JJ cord-008590-xivnsldf 78 43 variability variability NN cord-008590-xivnsldf 78 44 of of IN cord-008590-xivnsldf 78 45 the the DT cord-008590-xivnsldf 78 46 GC GC NNP cord-008590-xivnsldf 78 47 as as RB cord-008590-xivnsldf 78 48 well well RB cord-008590-xivnsldf 78 49 . . . cord-008590-xivnsldf 79 1 In in IN cord-008590-xivnsldf 79 2 fact fact NN cord-008590-xivnsldf 79 3 , , , cord-008590-xivnsldf 79 4 it -PRON- PRP cord-008590-xivnsldf 79 5 is be VBZ cord-008590-xivnsldf 79 6 clear clear JJ cord-008590-xivnsldf 79 7 that that IN cord-008590-xivnsldf 79 8 selected select VBN cord-008590-xivnsldf 79 9 sugar sugar NN cord-008590-xivnsldf 79 10 transferases transferase NNS cord-008590-xivnsldf 79 11 are be VBP cord-008590-xivnsldf 79 12 highly highly RB cord-008590-xivnsldf 79 13 enriched enrich VBN cord-008590-xivnsldf 79 14 only only RB cord-008590-xivnsldf 79 15 in in IN cord-008590-xivnsldf 79 16 certain certain JJ cord-008590-xivnsldf 79 17 tissues tissue NNS cord-008590-xivnsldf 79 18 . . . cord-008590-xivnsldf 80 1 There there EX cord-008590-xivnsldf 80 2 also also RB cord-008590-xivnsldf 80 3 must must MD cord-008590-xivnsldf 80 4 exist exist VB cord-008590-xivnsldf 80 5 a a DT cord-008590-xivnsldf 80 6 set set NN cord-008590-xivnsldf 80 7 of of IN cord-008590-xivnsldf 80 8 altogether altogether RB cord-008590-xivnsldf 80 9 constant constant JJ cord-008590-xivnsldf 80 10 Golgi Golgi NNP cord-008590-xivnsldf 80 11 components component NNS cord-008590-xivnsldf 80 12 - - : cord-008590-xivnsldf 80 13 those those DT cord-008590-xivnsldf 80 14 which which WDT cord-008590-xivnsldf 80 15 are be VBP cord-008590-xivnsldf 80 16 responsible responsible JJ cord-008590-xivnsldf 80 17 for for IN cord-008590-xivnsldf 80 18 its -PRON- PRP$ cord-008590-xivnsldf 80 19 characteristic characteristic JJ cord-008590-xivnsldf 80 20 structure structure NN cord-008590-xivnsldf 80 21 and and CC cord-008590-xivnsldf 80 22 endow endow VB cord-008590-xivnsldf 80 23 it -PRON- PRP cord-008590-xivnsldf 80 24 with with IN cord-008590-xivnsldf 80 25 the the DT cord-008590-xivnsldf 80 26 ability ability NN cord-008590-xivnsldf 80 27 to to TO cord-008590-xivnsldf 80 28 conduct conduct VB cord-008590-xivnsldf 80 29 transport transport NN cord-008590-xivnsldf 80 30 . . . cord-008590-xivnsldf 81 1 No no DT cord-008590-xivnsldf 81 2 such such JJ cord-008590-xivnsldf 81 3 components component NNS cord-008590-xivnsldf 81 4 have have VBP cord-008590-xivnsldf 81 5 yet yet RB cord-008590-xivnsldf 81 6 been be VBN cord-008590-xivnsldf 81 7 identified identify VBN cord-008590-xivnsldf 81 8 . . . cord-008590-xivnsldf 82 1 A a DT cord-008590-xivnsldf 82 2 secondary secondary JJ cord-008590-xivnsldf 82 3 task task NN cord-008590-xivnsldf 82 4 in in IN cord-008590-xivnsldf 82 5 this this DT cord-008590-xivnsldf 82 6 mapping mapping NN cord-008590-xivnsldf 82 7 of of IN cord-008590-xivnsldf 82 8 Golgi Golgi NNP cord-008590-xivnsldf 82 9 functions function NNS cord-008590-xivnsldf 82 10 is be VBZ cord-008590-xivnsldf 82 11 to to TO cord-008590-xivnsldf 82 12 position position VB cord-008590-xivnsldf 82 13 the the DT cord-008590-xivnsldf 82 14 processing processing NN cord-008590-xivnsldf 82 15 paths path NNS cord-008590-xivnsldf 82 16 one one CD cord-008590-xivnsldf 82 17 relative relative NN cord-008590-xivnsldf 82 18 to to IN cord-008590-xivnsldf 82 19 the the DT cord-008590-xivnsldf 82 20 next next JJ cord-008590-xivnsldf 82 21 . . . cord-008590-xivnsldf 83 1 For for IN cord-008590-xivnsldf 83 2 example example NN cord-008590-xivnsldf 83 3 , , , cord-008590-xivnsldf 83 4 is be VBZ cord-008590-xivnsldf 83 5 the the DT cord-008590-xivnsldf 83 6 compartment compartment NN cord-008590-xivnsldf 83 7 responsible responsible JJ cord-008590-xivnsldf 83 8 for for IN cord-008590-xivnsldf 83 9 proteoglycan proteoglycan NN cord-008590-xivnsldf 83 10 sulfation sulfation NN cord-008590-xivnsldf 83 11 the the DT cord-008590-xivnsldf 83 12 same same JJ cord-008590-xivnsldf 83 13 as as IN cord-008590-xivnsldf 83 14 that that DT cord-008590-xivnsldf 83 15 responsible responsible JJ cord-008590-xivnsldf 83 16 for for IN cord-008590-xivnsldf 83 17 galactose galactose NN cord-008590-xivnsldf 83 18 addition addition NN cord-008590-xivnsldf 83 19 to to IN cord-008590-xivnsldf 83 20 asparagine asparagine NN cord-008590-xivnsldf 83 21 - - HYPH cord-008590-xivnsldf 83 22 linked link VBN cord-008590-xivnsldf 83 23 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 83 24 ? ? . cord-008590-xivnsldf 84 1 The the DT cord-008590-xivnsldf 84 2 ideal ideal JJ cord-008590-xivnsldf 84 3 systems system NNS cord-008590-xivnsldf 84 4 to to TO cord-008590-xivnsldf 84 5 investigate investigate VB cord-008590-xivnsldf 84 6 to to TO cord-008590-xivnsldf 84 7 obtain obtain VB cord-008590-xivnsldf 84 8 such such JJ cord-008590-xivnsldf 84 9 information information NN cord-008590-xivnsldf 84 10 are be VBP cord-008590-xivnsldf 84 11 those those DT cord-008590-xivnsldf 84 12 in in IN cord-008590-xivnsldf 84 13 which which WDT cord-008590-xivnsldf 84 14 a a DT cord-008590-xivnsldf 84 15 single single JJ cord-008590-xivnsldf 84 16 polypeptide polypeptide NN cord-008590-xivnsldf 84 17 undergoes undergo VBZ cord-008590-xivnsldf 84 18 several several JJ cord-008590-xivnsldf 84 19 _SP cord-008590-xivnsldf 84 20 Numerous numerous JJ cord-008590-xivnsldf 84 21 secretory secretory NN cord-008590-xivnsldf 84 22 and and CC cord-008590-xivnsldf 84 23 membrane membrane NN cord-008590-xivnsldf 84 24 proteins protein NNS cord-008590-xivnsldf 84 25 are be VBP cord-008590-xivnsldf 84 26 selectively selectively RB cord-008590-xivnsldf 84 27 cleaved cleave VBN cord-008590-xivnsldf 84 28 during during IN cord-008590-xivnsldf 84 29 their -PRON- PRP$ cord-008590-xivnsldf 84 30 passage passage NN cord-008590-xivnsldf 84 31 through through IN cord-008590-xivnsldf 84 32 the the DT cord-008590-xivnsldf 84 33 GC GC NNP cord-008590-xivnsldf 84 34 to to IN cord-008590-xivnsldf 84 35 the the DT cord-008590-xivnsldf 84 36 cell cell NN cord-008590-xivnsldf 84 37 surface surface NN cord-008590-xivnsldf 84 38 . . . cord-008590-xivnsldf 85 1 The the DT cord-008590-xivnsldf 85 2 best well RBS cord-008590-xivnsldf 85 3 studied study VBN cord-008590-xivnsldf 85 4 examples example NNS cord-008590-xivnsldf 85 5 are be VBP cord-008590-xivnsldf 85 6 proalbumin proalbumin NNP cord-008590-xivnsldf 85 7 , , , cord-008590-xivnsldf 85 8 the the DT cord-008590-xivnsldf 85 9 spike spike NN cord-008590-xivnsldf 85 10 glycoproteins glycoprotein NNS cord-008590-xivnsldf 85 11 of of IN cord-008590-xivnsldf 85 12 many many JJ cord-008590-xivnsldf 85 13 enveloped envelop VBN cord-008590-xivnsldf 85 14 virus virus NN cord-008590-xivnsldf 85 15 , , , cord-008590-xivnsldf 85 16 and and CC cord-008590-xivnsldf 85 17 the the DT cord-008590-xivnsldf 85 18 family family NN cord-008590-xivnsldf 85 19 of of IN cord-008590-xivnsldf 85 20 precursors precursor NNS cord-008590-xivnsldf 85 21 of of IN cord-008590-xivnsldf 85 22 polypeptide polypeptide NN cord-008590-xivnsldf 85 23 hormones hormone NNS cord-008590-xivnsldf 85 24 [ [ -LRB- cord-008590-xivnsldf 85 25 pro pro JJ cord-008590-xivnsldf 85 26 - - JJ cord-008590-xivnsldf 85 27 insulin insulin JJ cord-008590-xivnsldf 85 28 , , , cord-008590-xivnsldf 85 29 -glucagon -glucagon NN cord-008590-xivnsldf 85 30 , , , cord-008590-xivnsldf 85 31 -parathyroid -parathyroid JJ cord-008590-xivnsldf 85 32 hormone hormone NN cord-008590-xivnsldf 85 33 , , , cord-008590-xivnsldf 85 34 -gastrin -gastrin : cord-008590-xivnsldf 85 35 , , , cord-008590-xivnsldf 85 36 -enkephalin -enkephalin JJ cord-008590-xivnsldf 85 37 ( ( -LRB- cord-008590-xivnsldf 85 38 14 14 CD cord-008590-xivnsldf 85 39 ) ) -RRB- cord-008590-xivnsldf 85 40 , , , cord-008590-xivnsldf 85 41 -opiomelanocortin -opiomelanocortin NNS cord-008590-xivnsldf 85 42 ( ( -LRB- cord-008590-xivnsldf 85 43 48 48 CD cord-008590-xivnsldf 85 44 ) ) -RRB- cord-008590-xivnsldf 85 45 , , , cord-008590-xivnsldf 85 46 vasopressin vasopressin NN cord-008590-xivnsldf 85 47 - - HYPH cord-008590-xivnsldf 85 48 neurophysin neurophysin NN cord-008590-xivnsldf 85 49 precursor precursor NN cord-008590-xivnsldf 85 50 ( ( -LRB- cord-008590-xivnsldf 85 51 74 74 CD cord-008590-xivnsldf 85 52 ) ) -RRB- cord-008590-xivnsldf 85 53 ] ] -RRB- cord-008590-xivnsldf 85 54 . . . cord-008590-xivnsldf 86 1 Many many JJ cord-008590-xivnsldf 86 2 of of IN cord-008590-xivnsldf 86 3 the the DT cord-008590-xivnsldf 86 4 data datum NNS cord-008590-xivnsldf 86 5 implicating implicate VBG cord-008590-xivnsldf 86 6 the the DT cord-008590-xivnsldf 86 7 GC GC NNP cord-008590-xivnsldf 86 8 are be VBP cord-008590-xivnsldf 86 9 in in IN cord-008590-xivnsldf 86 10 fact fact NN cord-008590-xivnsldf 86 11 only only RB cord-008590-xivnsldf 86 12 indirect indirect JJ cord-008590-xivnsldf 86 13 . . . cord-008590-xivnsldf 87 1 The the DT cord-008590-xivnsldf 87 2 approaches approach NNS cord-008590-xivnsldf 87 3 used use VBN cord-008590-xivnsldf 87 4 involved involved JJ cord-008590-xivnsldf 87 5 study study NN cord-008590-xivnsldf 87 6 of of IN cord-008590-xivnsldf 87 7 the the DT cord-008590-xivnsldf 87 8 effect effect NN cord-008590-xivnsldf 87 9 of of IN cord-008590-xivnsldf 87 10 agents agent NNS cord-008590-xivnsldf 87 11 which which WDT cord-008590-xivnsldf 87 12 block block VBP cord-008590-xivnsldf 87 13 secretory secretory JJ cord-008590-xivnsldf 87 14 protein protein NN cord-008590-xivnsldf 87 15 exit exit NN cord-008590-xivnsldf 87 16 from from IN cord-008590-xivnsldf 87 17 the the DT cord-008590-xivnsldf 87 18 RER RER NNP cord-008590-xivnsldf 87 19 ( ( -LRB- cord-008590-xivnsldf 87 20 e.g. e.g. RB cord-008590-xivnsldf 87 21 , , , cord-008590-xivnsldf 87 22 uncouplers uncoupler NNS cord-008590-xivnsldf 87 23 ) ) -RRB- cord-008590-xivnsldf 87 24 ( ( -LRB- cord-008590-xivnsldf 87 25 130 130 CD cord-008590-xivnsldf 87 26 ) ) -RRB- cord-008590-xivnsldf 87 27 , , , cord-008590-xivnsldf 87 28 correlation correlation NN cord-008590-xivnsldf 87 29 between between IN cord-008590-xivnsldf 87 30 the the DT cord-008590-xivnsldf 87 31 kinetics kinetic NNS cord-008590-xivnsldf 87 32 of of IN cord-008590-xivnsldf 87 33 ICT ICT NNP cord-008590-xivnsldf 87 34 and and CC cord-008590-xivnsldf 87 35 cleavage cleavage NN cord-008590-xivnsldf 87 36 , , , cord-008590-xivnsldf 87 37 or or CC cord-008590-xivnsldf 87 38 , , , cord-008590-xivnsldf 87 39 best good JJS cord-008590-xivnsldf 87 40 of of IN cord-008590-xivnsldf 87 41 all all DT cord-008590-xivnsldf 87 42 , , , cord-008590-xivnsldf 87 43 analysis analysis NN cord-008590-xivnsldf 87 44 of of IN cord-008590-xivnsldf 87 45 smooth smooth JJ cord-008590-xivnsldf 87 46 microsomal microsomal NNS cord-008590-xivnsldf 87 47 or or CC cord-008590-xivnsldf 87 48 Golgi golgi NN cord-008590-xivnsldf 87 49 - - HYPH cord-008590-xivnsldf 87 50 enriched enrich VBN cord-008590-xivnsldf 87 51 subcellular subcellular JJ cord-008590-xivnsldf 87 52 fractions fraction NNS cord-008590-xivnsldf 87 53 [ [ -LRB- cord-008590-xivnsldf 87 54 e.g. e.g. RB cord-008590-xivnsldf 87 55 , , , cord-008590-xivnsldf 87 56 of of IN cord-008590-xivnsldf 87 57 hepatocytes hepatocyte NNS cord-008590-xivnsldf 87 58 ( ( -LRB- cord-008590-xivnsldf 87 59 92 92 CD cord-008590-xivnsldf 87 60 , , , cord-008590-xivnsldf 87 61 102 102 CD cord-008590-xivnsldf 87 62 ) ) -RRB- cord-008590-xivnsldf 87 63 or or CC cord-008590-xivnsldf 87 64 virally virally RB cord-008590-xivnsldf 87 65 infected infect VBN cord-008590-xivnsldf 87 66 cells cell NNS cord-008590-xivnsldf 87 67 ( ( -LRB- cord-008590-xivnsldf 87 68 68 68 CD cord-008590-xivnsldf 87 69 ) ) -RRB- cord-008590-xivnsldf 87 70 ] ] -RRB- cord-008590-xivnsldf 87 71 . . . cord-008590-xivnsldf 88 1 In in IN cord-008590-xivnsldf 88 2 the the DT cord-008590-xivnsldf 88 3 case case NN cord-008590-xivnsldf 88 4 of of IN cord-008590-xivnsldf 88 5 Sindbis Sindbis NNP cord-008590-xivnsldf 88 6 and and CC cord-008590-xivnsldf 88 7 Semliki Semliki NNP cord-008590-xivnsldf 88 8 Forest Forest NNP cord-008590-xivnsldf 88 9 virus virus NN cord-008590-xivnsldf 88 10 it -PRON- PRP cord-008590-xivnsldf 88 11 has have VBZ cord-008590-xivnsldf 88 12 been be VBN cord-008590-xivnsldf 88 13 suggested suggest VBN cord-008590-xivnsldf 88 14 that that IN cord-008590-xivnsldf 88 15 cleavage cleavage NN cord-008590-xivnsldf 88 16 occurs occur VBZ cord-008590-xivnsldf 88 17 at at IN cord-008590-xivnsldf 88 18 the the DT cord-008590-xivnsldf 88 19 moment moment NN cord-008590-xivnsldf 88 20 of of IN cord-008590-xivnsldf 88 21 arrival arrival NN cord-008590-xivnsldf 88 22 at at IN cord-008590-xivnsldf 88 23 the the DT cord-008590-xivnsldf 88 24 cell cell NN cord-008590-xivnsldf 88 25 surface surface NN cord-008590-xivnsldf 88 26 since since IN cord-008590-xivnsldf 88 27 extracellular extracellular JJ cord-008590-xivnsldf 88 28 antisera antisera NN cord-008590-xivnsldf 88 29 directed direct VBN cord-008590-xivnsldf 88 30 against against IN cord-008590-xivnsldf 88 31 their -PRON- PRP$ cord-008590-xivnsldf 88 32 glycoproteins glycoprotein NNS cord-008590-xivnsldf 88 33 can can MD cord-008590-xivnsldf 88 34 block block VB cord-008590-xivnsldf 88 35 cleavage cleavage NN cord-008590-xivnsldf 88 36 and and CC cord-008590-xivnsldf 88 37 since since IN cord-008590-xivnsldf 88 38 precursor precursor NN cord-008590-xivnsldf 88 39 species specie NNS cord-008590-xivnsldf 88 40 are be VBP cord-008590-xivnsldf 88 41 not not RB cord-008590-xivnsldf 88 42 detected detect VBN cord-008590-xivnsldf 88 43 by by IN cord-008590-xivnsldf 88 44 surface surface NN cord-008590-xivnsldf 88 45 - - HYPH cord-008590-xivnsldf 88 46 labeling labeling NN cord-008590-xivnsldf 88 47 procedures procedure NNS cord-008590-xivnsldf 88 48 ( ( -LRB- cord-008590-xivnsldf 88 49 8,15 8,15 CD cord-008590-xivnsldf 88 50 1 1 CD cord-008590-xivnsldf 88 51 ) ) -RRB- cord-008590-xivnsldf 88 52 . . . cord-008590-xivnsldf 89 1 However however RB cord-008590-xivnsldf 89 2 , , , cord-008590-xivnsldf 89 3 the the DT cord-008590-xivnsldf 89 4 cytologic cytologic JJ cord-008590-xivnsldf 89 5 site site NN cord-008590-xivnsldf 89 6 of of IN cord-008590-xivnsldf 89 7 cleavage cleavage NN cord-008590-xivnsldf 89 8 may may MD cord-008590-xivnsldf 89 9 depend depend VB cord-008590-xivnsldf 89 10 on on IN cord-008590-xivnsldf 89 11 both both CC cord-008590-xivnsldf 89 12 the the DT cord-008590-xivnsldf 89 13 virus virus NN cord-008590-xivnsldf 89 14 and and CC cord-008590-xivnsldf 89 15 the the DT cord-008590-xivnsldf 89 16 host host NN cord-008590-xivnsldf 89 17 cell cell NN cord-008590-xivnsldf 89 18 - - : cord-008590-xivnsldf 89 19 for for IN cord-008590-xivnsldf 89 20 several several JJ cord-008590-xivnsldf 89 21 paramyxovirus paramyxovirus NN cord-008590-xivnsldf 89 22 , , , cord-008590-xivnsldf 89 23 influenza influenza NN cord-008590-xivnsldf 89 24 virus virus NN cord-008590-xivnsldf 89 25 and and CC cord-008590-xivnsldf 89 26 certain certain JJ cord-008590-xivnsldf 89 27 RNA RNA NNP cord-008590-xivnsldf 89 28 tumor tumor NN cord-008590-xivnsldf 89 29 virus virus NN cord-008590-xivnsldf 89 30 cleavage cleavage NN cord-008590-xivnsldf 89 31 has have VBZ cord-008590-xivnsldf 89 32 been be VBN cord-008590-xivnsldf 89 33 reported report VBN cord-008590-xivnsldf 89 34 to to TO cord-008590-xivnsldf 89 35 occur occur VB cord-008590-xivnsldf 89 36 intracellularly intracellularly NNP cord-008590-xivnsldf 89 37 ( ( -LRB- cord-008590-xivnsldf 89 38 68 68 CD cord-008590-xivnsldf 89 39 ) ) -RRB- cord-008590-xivnsldf 89 40 . . . cord-008590-xivnsldf 90 1 For for IN cord-008590-xivnsldf 90 2 Rous rous JJ cord-008590-xivnsldf 90 3 sarcoma sarcoma NN cord-008590-xivnsldf 90 4 virus virus NN cord-008590-xivnsldf 90 5 the the DT cord-008590-xivnsldf 90 6 suggested suggest VBN cord-008590-xivnsldf 90 7 site site NN cord-008590-xivnsldf 90 8 of of IN cord-008590-xivnsldf 90 9 cleavage cleavage NN cord-008590-xivnsldf 90 10 ranges range NNS cord-008590-xivnsldf 90 11 from from IN cord-008590-xivnsldf 90 12 the the DT cord-008590-xivnsldf 90 13 GC GC NNP cord-008590-xivnsldf 90 14 to to IN cord-008590-xivnsldf 90 15 the the DT cord-008590-xivnsldf 90 16 virion virion NN cord-008590-xivnsldf 90 17 itself -PRON- PRP cord-008590-xivnsldf 90 18 ( ( -LRB- cord-008590-xivnsldf 90 19 7a 7a NN cord-008590-xivnsldf 90 20 ) ) -RRB- cord-008590-xivnsldf 90 21 . . . cord-008590-xivnsldf 91 1 Moreover moreover RB cord-008590-xivnsldf 91 2 , , , cord-008590-xivnsldf 91 3 certain certain JJ cord-008590-xivnsldf 91 4 host host NN cord-008590-xivnsldf 91 5 cells cell NNS cord-008590-xivnsldf 91 6 do do VBP cord-008590-xivnsldf 91 7 not not RB cord-008590-xivnsldf 91 8 cleave cleave VB cord-008590-xivnsldf 91 9 the the DT cord-008590-xivnsldf 91 10 viral viral JJ cord-008590-xivnsldf 91 11 glycoproteins glycoprotein NNS cord-008590-xivnsldf 91 12 and and CC cord-008590-xivnsldf 91 13 therefore therefore RB cord-008590-xivnsldf 91 14 may may MD cord-008590-xivnsldf 91 15 yield yield VB cord-008590-xivnsldf 91 16 noninfectious noninfectious JJ cord-008590-xivnsldf 91 17 virus virus NN cord-008590-xivnsldf 91 18 . . . cord-008590-xivnsldf 92 1 The the DT cord-008590-xivnsldf 92 2 covalent covalent JJ cord-008590-xivnsldf 92 3 site site NN cord-008590-xivnsldf 92 4 of of IN cord-008590-xivnsldf 92 5 proteolysis proteolysis NN cord-008590-xivnsldf 92 6 is be VBZ cord-008590-xivnsldf 92 7 strikingly strikingly RB cord-008590-xivnsldf 92 8 uniform uniform JJ cord-008590-xivnsldf 92 9 ( ( -LRB- cord-008590-xivnsldf 92 10 18 18 CD cord-008590-xivnsldf 92 11 , , , cord-008590-xivnsldf 92 12 32 32 CD cord-008590-xivnsldf 92 13 ) ) -RRB- cord-008590-xivnsldf 92 14 . . . cord-008590-xivnsldf 93 1 In in IN cord-008590-xivnsldf 93 2 all all PDT cord-008590-xivnsldf 93 3 the the DT cord-008590-xivnsldf 93 4 cases case NNS cord-008590-xivnsldf 93 5 mentioned mention VBN cord-008590-xivnsldf 93 6 above above RB cord-008590-xivnsldf 93 7 , , , cord-008590-xivnsldf 93 8 cleavage cleavage NN cord-008590-xivnsldf 93 9 occurs occur VBZ cord-008590-xivnsldf 93 10 adjacent adjacent JJ cord-008590-xivnsldf 93 11 to to IN cord-008590-xivnsldf 93 12 one one CD cord-008590-xivnsldf 93 13 to to TO cord-008590-xivnsldf 93 14 four four CD cord-008590-xivnsldf 93 15 basic basic JJ cord-008590-xivnsldf 93 16 amino amino NN cord-008590-xivnsldf 93 17 acids acid NNS cord-008590-xivnsldf 93 18 and and CC cord-008590-xivnsldf 93 19 results result NNS cord-008590-xivnsldf 93 20 in in IN cord-008590-xivnsldf 93 21 the the DT cord-008590-xivnsldf 93 22 release release NN cord-008590-xivnsldf 93 23 of of IN cord-008590-xivnsldf 93 24 lysine lysine NN cord-008590-xivnsldf 93 25 or or CC cord-008590-xivnsldf 93 26 arginine arginine NN cord-008590-xivnsldf 93 27 . . . cord-008590-xivnsldf 94 1 Such such JJ cord-008590-xivnsldf 94 2 amino amino NN cord-008590-xivnsldf 94 3 acid acid NN cord-008590-xivnsldf 94 4 excision excision NN cord-008590-xivnsldf 94 5 and and CC cord-008590-xivnsldf 94 6 the the DT cord-008590-xivnsldf 94 7 generation generation NN cord-008590-xivnsldf 94 8 , , , cord-008590-xivnsldf 94 9 for for IN cord-008590-xivnsldf 94 10 example example NN cord-008590-xivnsldf 94 11 , , , cord-008590-xivnsldf 94 12 of of IN cord-008590-xivnsldf 94 13 insulin insulin NN cord-008590-xivnsldf 94 14 from from IN cord-008590-xivnsldf 94 15 proinsulin proinsulin NNP cord-008590-xivnsldf 94 16 , , , cord-008590-xivnsldf 94 17 have have VBP cord-008590-xivnsldf 94 18 been be VBN cord-008590-xivnsldf 94 19 ascribed ascribe VBN cord-008590-xivnsldf 94 20 to to IN cord-008590-xivnsldf 94 21 the the DT cord-008590-xivnsldf 94 22 concerted concerted JJ cord-008590-xivnsldf 94 23 action action NN cord-008590-xivnsldf 94 24 of of IN cord-008590-xivnsldf 94 25 both both DT cord-008590-xivnsldf 94 26 trypsin trypsin NN cord-008590-xivnsldf 94 27 - - HYPH cord-008590-xivnsldf 94 28 like like JJ cord-008590-xivnsldf 94 29 and and CC cord-008590-xivnsldf 94 30 carboxypeptidase carboxypeptidase NN cord-008590-xivnsldf 94 31 B b NN cord-008590-xivnsldf 94 32 - - HYPH cord-008590-xivnsldf 94 33 like like JJ cord-008590-xivnsldf 94 34 activities activity NNS cord-008590-xivnsldf 94 35 ( ( -LRB- cord-008590-xivnsldf 94 36 18 18 CD cord-008590-xivnsldf 94 37 ) ) -RRB- cord-008590-xivnsldf 94 38 , , , cord-008590-xivnsldf 94 39 however however RB cord-008590-xivnsldf 94 40 , , , cord-008590-xivnsldf 94 41 none none NN cord-008590-xivnsldf 94 42 of of IN cord-008590-xivnsldf 94 43 the the DT cord-008590-xivnsldf 94 44 claims claim NNS cord-008590-xivnsldf 94 45 of of IN cord-008590-xivnsldf 94 46 attribution attribution NN cord-008590-xivnsldf 94 47 of of IN cord-008590-xivnsldf 94 48 these these DT cord-008590-xivnsldf 94 49 activities activity NNS cord-008590-xivnsldf 94 50 to to IN cord-008590-xivnsldf 94 51 identifiable identifiable JJ cord-008590-xivnsldf 94 52 enzymes enzyme NNS cord-008590-xivnsldf 94 53 is be VBZ cord-008590-xivnsldf 94 54 unequivocally unequivocally RB cord-008590-xivnsldf 94 55 accepted accept VBN cord-008590-xivnsldf 94 56 . . . cord-008590-xivnsldf 95 1 Such such JJ cord-008590-xivnsldf 95 2 protease protease NN cord-008590-xivnsldf 95 3 activities activity NNS cord-008590-xivnsldf 95 4 have have VBP cord-008590-xivnsldf 95 5 yet yet RB cord-008590-xivnsldf 95 6 to to TO cord-008590-xivnsldf 95 7 be be VB cord-008590-xivnsldf 95 8 used use VBN cord-008590-xivnsldf 95 9 as as IN cord-008590-xivnsldf 95 10 Golgi Golgi NNP cord-008590-xivnsldf 95 11 markers marker NNS cord-008590-xivnsldf 95 12 . . . cord-008590-xivnsldf 96 1 An an DT cord-008590-xivnsldf 96 2 outstanding outstanding JJ cord-008590-xivnsldf 96 3 example example NN cord-008590-xivnsldf 96 4 of of IN cord-008590-xivnsldf 96 5 differential differential JJ cord-008590-xivnsldf 96 6 cleavage cleavage NN cord-008590-xivnsldf 96 7 is be VBZ cord-008590-xivnsldf 96 8 the the DT cord-008590-xivnsldf 96 9 case case NN cord-008590-xivnsldf 96 10 of of IN cord-008590-xivnsldf 96 11 proopiomelanocortin proopiomelanocortin NNS cord-008590-xivnsldf 96 12 . . . cord-008590-xivnsldf 97 1 The the DT cord-008590-xivnsldf 97 2 same same JJ cord-008590-xivnsldf 97 3 precursor precursor NN cord-008590-xivnsldf 97 4 is be VBZ cord-008590-xivnsldf 97 5 cleaved cleave VBN cord-008590-xivnsldf 97 6 ( ( -LRB- cord-008590-xivnsldf 97 7 always always RB cord-008590-xivnsldf 97 8 adjacent adjacent JJ cord-008590-xivnsldf 97 9 to to IN cord-008590-xivnsldf 97 10 basic basic JJ cord-008590-xivnsldf 97 11 amino amino NN cord-008590-xivnsldf 97 12 acids acid NNS cord-008590-xivnsldf 97 13 ) ) -RRB- cord-008590-xivnsldf 97 14 to to TO cord-008590-xivnsldf 97 15 yield yield VB cord-008590-xivnsldf 97 16 different different JJ cord-008590-xivnsldf 97 17 products product NNS cord-008590-xivnsldf 97 18 by by IN cord-008590-xivnsldf 97 19 cells cell NNS cord-008590-xivnsldf 97 20 of of IN cord-008590-xivnsldf 97 21 the the DT cord-008590-xivnsldf 97 22 anterior anterior JJ cord-008590-xivnsldf 97 23 or or CC cord-008590-xivnsldf 97 24 intermediary intermediary JJ cord-008590-xivnsldf 97 25 lobe lobe NN cord-008590-xivnsldf 97 26 of of IN cord-008590-xivnsldf 97 27 the the DT cord-008590-xivnsldf 97 28 pituitary pituitary NN cord-008590-xivnsldf 97 29 ( ( -LRB- cord-008590-xivnsldf 97 30 46 46 CD cord-008590-xivnsldf 97 31 , , , cord-008590-xivnsldf 97 32 48 48 CD cord-008590-xivnsldf 97 33 ) ) -RRB- cord-008590-xivnsldf 97 34 . . . cord-008590-xivnsldf 98 1 Moreover moreover RB cord-008590-xivnsldf 98 2 , , , cord-008590-xivnsldf 98 3 in in IN cord-008590-xivnsldf 98 4 other other JJ cord-008590-xivnsldf 98 5 endocrine endocrine JJ cord-008590-xivnsldf 98 6 cells cell NNS cord-008590-xivnsldf 98 7 minor minor JJ cord-008590-xivnsldf 98 8 amounts amount NNS cord-008590-xivnsldf 98 9 of of IN cord-008590-xivnsldf 98 10 unorthodox unorthodox JJ cord-008590-xivnsldf 98 11 or or CC cord-008590-xivnsldf 98 12 incomplete incomplete JJ cord-008590-xivnsldf 98 13 cleavage cleavage NN cord-008590-xivnsldf 98 14 products product NNS cord-008590-xivnsldf 98 15 may may MD cord-008590-xivnsldf 98 16 be be VB cord-008590-xivnsldf 98 17 detectable detectable JJ cord-008590-xivnsldf 98 18 ( ( -LRB- cord-008590-xivnsldf 98 19 18 18 CD cord-008590-xivnsldf 98 20 ) ) -RRB- cord-008590-xivnsldf 98 21 . . . cord-008590-xivnsldf 99 1 The the DT cord-008590-xivnsldf 99 2 result result NN cord-008590-xivnsldf 99 3 is be VBZ cord-008590-xivnsldf 99 4 a a DT cord-008590-xivnsldf 99 5 variety variety NN cord-008590-xivnsldf 99 6 of of IN cord-008590-xivnsldf 99 7 microheterogeneity microheterogeneity NN cord-008590-xivnsldf 99 8 reminiscent reminiscent JJ cord-008590-xivnsldf 99 9 of of IN cord-008590-xivnsldf 99 10 the the DT cord-008590-xivnsldf 99 11 microheterogeneity microheterogeneity NN cord-008590-xivnsldf 99 12 of of IN cord-008590-xivnsldf 99 13 processed process VBN cord-008590-xivnsldf 99 14 carbohydrate carbohydrate NN cord-008590-xivnsldf 99 15 structures structure NNS cord-008590-xivnsldf 99 16 of of IN cord-008590-xivnsldf 99 17 glycoproteins glycoprotein NNS cord-008590-xivnsldf 99 18 . . . cord-008590-xivnsldf 100 1 It -PRON- PRP cord-008590-xivnsldf 100 2 has have VBZ cord-008590-xivnsldf 100 3 been be VBN cord-008590-xivnsldf 100 4 suggested suggest VBN cord-008590-xivnsldf 100 5 that that IN cord-008590-xivnsldf 100 6 the the DT cord-008590-xivnsldf 100 7 proteolysis proteolysis NN cord-008590-xivnsldf 100 8 of of IN cord-008590-xivnsldf 100 9 proinsulin proinsulin NNP cord-008590-xivnsldf 100 10 to to IN cord-008590-xivnsldf 100 11 insulin insulin NN cord-008590-xivnsldf 100 12 may may MD cord-008590-xivnsldf 100 13 occur occur VB cord-008590-xivnsldf 100 14 both both DT cord-008590-xivnsldf 100 15 within within IN cord-008590-xivnsldf 100 16 the the DT cord-008590-xivnsldf 100 17 GC GC NNP cord-008590-xivnsldf 100 18 and and CC cord-008590-xivnsldf 100 19 in in IN cord-008590-xivnsldf 100 20 secretion secretion NN cord-008590-xivnsldf 100 21 granules granule NNS cord-008590-xivnsldf 100 22 ( ( -LRB- cord-008590-xivnsldf 100 23 18 18 CD cord-008590-xivnsldf 100 24 , , , cord-008590-xivnsldf 100 25 130 130 CD cord-008590-xivnsldf 100 26 ) ) -RRB- cord-008590-xivnsldf 100 27 . . . cord-008590-xivnsldf 101 1 The the DT cord-008590-xivnsldf 101 2 experiments experiment NNS cord-008590-xivnsldf 101 3 on on IN cord-008590-xivnsldf 101 4 which which WDT cord-008590-xivnsldf 101 5 this this DT cord-008590-xivnsldf 101 6 idea idea NN cord-008590-xivnsldf 101 7 is be VBZ cord-008590-xivnsldf 101 8 based base VBN cord-008590-xivnsldf 101 9 involve involve JJ cord-008590-xivnsldf 101 10 study study NN cord-008590-xivnsldf 101 11 of of IN cord-008590-xivnsldf 101 12 isolated isolate VBN cord-008590-xivnsldf 101 13 granule granule NN cord-008590-xivnsldf 101 14 fractions fraction NNS cord-008590-xivnsldf 101 15 derived derive VBN cord-008590-xivnsldf 101 16 from from IN cord-008590-xivnsldf 101 17 biosynthetically biosynthetically RB cord-008590-xivnsldf 101 18 labeled label VBN cord-008590-xivnsldf 101 19 tissue tissue NN cord-008590-xivnsldf 101 20 . . . cord-008590-xivnsldf 102 1 These these DT cord-008590-xivnsldf 102 2 data datum NNS cord-008590-xivnsldf 102 3 suggest suggest VBP cord-008590-xivnsldf 102 4 that that IN cord-008590-xivnsldf 102 5 protease(s protease(s NNP cord-008590-xivnsldf 102 6 ) ) -RRB- cord-008590-xivnsldf 102 7 of of IN cord-008590-xivnsldf 102 8 the the DT cord-008590-xivnsldf 102 9 GC GC NNP cord-008590-xivnsldf 102 10 may may MD cord-008590-xivnsldf 102 11 be be VB cord-008590-xivnsldf 102 12 transported transport VBN cord-008590-xivnsldf 102 13 along along RB cord-008590-xivnsldf 102 14 with with IN cord-008590-xivnsldf 102 15 the the DT cord-008590-xivnsldf 102 16 secretory secretory JJ cord-008590-xivnsldf 102 17 product product NN cord-008590-xivnsldf 102 18 . . . cord-008590-xivnsldf 103 1 In in IN cord-008590-xivnsldf 103 2 the the DT cord-008590-xivnsldf 103 3 case case NN cord-008590-xivnsldf 103 4 of of IN cord-008590-xivnsldf 103 5 proopiomelanocortin proopiomelanocortin NNS cord-008590-xivnsldf 103 6 processing processing NN cord-008590-xivnsldf 103 7 , , , cord-008590-xivnsldf 103 8 successive successive JJ cord-008590-xivnsldf 103 9 cleavage cleavage NN cord-008590-xivnsldf 103 10 events event NNS cord-008590-xivnsldf 103 11 may may MD cord-008590-xivnsldf 103 12 occur occur VB cord-008590-xivnsldf 103 13 within within IN cord-008590-xivnsldf 103 14 different different JJ cord-008590-xivnsldf 103 15 compartments compartment NNS cord-008590-xivnsldf 103 16 - - HYPH cord-008590-xivnsldf 103 17 P p NN cord-008590-xivnsldf 103 18 - - HYPH cord-008590-xivnsldf 103 19 lipotropin lipotropin NNP cord-008590-xivnsldf 103 20 and and CC cord-008590-xivnsldf 103 21 ACTH ACTH NNP cord-008590-xivnsldf 103 22 are be VBP cord-008590-xivnsldf 103 23 generated generate VBN cord-008590-xivnsldf 103 24 during during IN cord-008590-xivnsldf 103 25 transport transport NN cord-008590-xivnsldf 103 26 , , , cord-008590-xivnsldf 103 27 while while IN cord-008590-xivnsldf 103 28 further further JJ cord-008590-xivnsldf 103 29 conversion conversion NN cord-008590-xivnsldf 103 30 to to IN cord-008590-xivnsldf 103 31 P p NN cord-008590-xivnsldf 103 32 - - HYPH cord-008590-xivnsldf 103 33 endorphin endorphin NN cord-008590-xivnsldf 103 34 occurs occur VBZ cord-008590-xivnsldf 103 35 in in IN cord-008590-xivnsldf 103 36 granules granule NNS cord-008590-xivnsldf 103 37 ( ( -LRB- cord-008590-xivnsldf 103 38 32a 32a NNS cord-008590-xivnsldf 103 39 , , , cord-008590-xivnsldf 103 40 38 38 CD cord-008590-xivnsldf 103 41 ) ) -RRB- cord-008590-xivnsldf 103 42 . . . cord-008590-xivnsldf 104 1 There there EX cord-008590-xivnsldf 104 2 are be VBP cord-008590-xivnsldf 104 3 no no DT cord-008590-xivnsldf 104 4 indications indication NNS cord-008590-xivnsldf 104 5 that that IN cord-008590-xivnsldf 104 6 cleavage cleavage NN cord-008590-xivnsldf 104 7 is be VBZ cord-008590-xivnsldf 104 8 a a DT cord-008590-xivnsldf 104 9 prerequisite prerequisite NN cord-008590-xivnsldf 104 10 for for IN cord-008590-xivnsldf 104 11 transport transport NN cord-008590-xivnsldf 104 12 . . . cord-008590-xivnsldf 105 1 In in IN cord-008590-xivnsldf 105 2 the the DT cord-008590-xivnsldf 105 3 case case NN cord-008590-xivnsldf 105 4 of of IN cord-008590-xivnsldf 105 5 proalbumin proalbumin NNP cord-008590-xivnsldf 105 6 , , , cord-008590-xivnsldf 105 7 proinsulin proinsulin NNP cord-008590-xivnsldf 105 8 , , , cord-008590-xivnsldf 105 9 and and CC cord-008590-xivnsldf 105 10 the the DT cord-008590-xivnsldf 105 11 precursors precursor NNS cord-008590-xivnsldf 105 12 of of IN cord-008590-xivnsldf 105 13 viral viral JJ cord-008590-xivnsldf 105 14 proteins protein NNS cord-008590-xivnsldf 105 15 , , , cord-008590-xivnsldf 105 16 altered alter VBN cord-008590-xivnsldf 105 17 polypeptides polypeptide NNS cord-008590-xivnsldf 105 18 which which WDT cord-008590-xivnsldf 105 19 resist resist VBP cord-008590-xivnsldf 105 20 cleavage cleavage NN cord-008590-xivnsldf 105 21 ( ( -LRB- cord-008590-xivnsldf 105 22 mutant mutant JJ cord-008590-xivnsldf 105 23 species specie NNS cord-008590-xivnsldf 105 24 or or CC cord-008590-xivnsldf 105 25 the the DT cord-008590-xivnsldf 105 26 products product NNS cord-008590-xivnsldf 105 27 of of IN cord-008590-xivnsldf 105 28 incorporation incorporation NN cord-008590-xivnsldf 105 29 of of IN cord-008590-xivnsldf 105 30 amino amino JJ cord-008590-xivnsldf 105 31 acid acid JJ cord-008590-xivnsldf 105 32 analogs analog NNS cord-008590-xivnsldf 105 33 ) ) -RRB- cord-008590-xivnsldf 105 34 have have VBP cord-008590-xivnsldf 105 35 been be VBN cord-008590-xivnsldf 105 36 shown show VBN cord-008590-xivnsldf 105 37 to to TO cord-008590-xivnsldf 105 38 arrive arrive VB cord-008590-xivnsldf 105 39 at at IN cord-008590-xivnsldf 105 40 the the DT cord-008590-xivnsldf 105 41 cell cell NN cord-008590-xivnsldf 105 42 surface surface NN cord-008590-xivnsldf 105 43 ( ( -LRB- cord-008590-xivnsldf 105 44 18 18 CD cord-008590-xivnsldf 105 45 , , , cord-008590-xivnsldf 105 46 68 68 CD cord-008590-xivnsldf 105 47 ) ) -RRB- cord-008590-xivnsldf 105 48 . . . cord-008590-xivnsldf 106 1 Amino amino NN cord-008590-xivnsldf 106 2 acid acid NN cord-008590-xivnsldf 106 3 labeling labeling NN cord-008590-xivnsldf 106 4 pulse pulse NN cord-008590-xivnsldf 106 5 - - HYPH cord-008590-xivnsldf 106 6 chase chase NN cord-008590-xivnsldf 106 7 experiments experiment NNS cord-008590-xivnsldf 106 8 show show VBP cord-008590-xivnsldf 106 9 that that IN cord-008590-xivnsldf 106 10 monensin monensin NNP cord-008590-xivnsldf 106 11 interrupts interrupt VBZ cord-008590-xivnsldf 106 12 the the DT cord-008590-xivnsldf 106 13 cleavage cleavage NN cord-008590-xivnsldf 106 14 of of IN cord-008590-xivnsldf 106 15 viral viral JJ cord-008590-xivnsldf 106 16 surface surface NN cord-008590-xivnsldf 106 17 glycoproteins glycoprotein NNS cord-008590-xivnsldf 106 18 , , , cord-008590-xivnsldf 106 19 proalbumin proalbumin NNP cord-008590-xivnsldf 106 20 , , , cord-008590-xivnsldf 106 21 and and CC cord-008590-xivnsldf 106 22 proopiomelanocortin proopiomelanocortin NNP cord-008590-xivnsldf 106 23 . . . cord-008590-xivnsldf 107 1 ( ( -LRB- cord-008590-xivnsldf 107 2 Table table NN cord-008590-xivnsldf 107 3 I i CD cord-008590-xivnsldf 107 4 ) ) -RRB- cord-008590-xivnsldf 107 5 . . . cord-008590-xivnsldf 108 1 Therefore therefore RB cord-008590-xivnsldf 108 2 , , , cord-008590-xivnsldf 108 3 one one PRP cord-008590-xivnsldf 108 4 can can MD cord-008590-xivnsldf 108 5 ascribe ascribe VB cord-008590-xivnsldf 108 6 cleavage cleavage NN cord-008590-xivnsldf 108 7 to to IN cord-008590-xivnsldf 108 8 a a DT cord-008590-xivnsldf 108 9 late late JJ cord-008590-xivnsldf 108 10 subcompartnient subcompartnient NN cord-008590-xivnsldf 108 11 . . . cord-008590-xivnsldf 109 1 This this DT cord-008590-xivnsldf 109 2 conclusion conclusion NN cord-008590-xivnsldf 109 3 is be VBZ cord-008590-xivnsldf 109 4 consistent consistent JJ cord-008590-xivnsldf 109 5 with with IN cord-008590-xivnsldf 109 6 the the DT cord-008590-xivnsldf 109 7 liver liver NN cord-008590-xivnsldf 109 8 Golgi Golgi NNP cord-008590-xivnsldf 109 9 subfractionation subfractionation NN cord-008590-xivnsldf 109 10 studies study NNS cord-008590-xivnsldf 109 11 which which WDT cord-008590-xivnsldf 109 12 show show VBP cord-008590-xivnsldf 109 13 that that IN cord-008590-xivnsldf 109 14 proalbumin proalbumin NN cord-008590-xivnsldf 109 15 - - HYPH cord-008590-xivnsldf 109 16 albumin albumin NN cord-008590-xivnsldf 109 17 conversion conversion NN cord-008590-xivnsldf 109 18 occurs occur VBZ cord-008590-xivnsldf 109 19 upon upon IN cord-008590-xivnsldf 109 20 entry entry NN cord-008590-xivnsldf 109 21 into into IN cord-008590-xivnsldf 109 22 ' ' '' cord-008590-xivnsldf 109 23 ' ' `` cord-008590-xivnsldf 109 24 postcisternal postcisternal JJ cord-008590-xivnsldf 109 25 " " '' cord-008590-xivnsldf 109 26 Golgi Golgi NNP cord-008590-xivnsldf 109 27 elements element NNS cord-008590-xivnsldf 109 28 - - : cord-008590-xivnsldf 109 29 those those DT cord-008590-xivnsldf 109 30 which which WDT cord-008590-xivnsldf 109 31 carry carry VBP cord-008590-xivnsldf 109 32 the the DT cord-008590-xivnsldf 109 33 greatest great JJS cord-008590-xivnsldf 109 34 load load NN cord-008590-xivnsldf 109 35 of of IN cord-008590-xivnsldf 109 36 lipoprotein lipoprotein NN cord-008590-xivnsldf 109 37 ( ( -LRB- cord-008590-xivnsldf 109 38 92 92 CD cord-008590-xivnsldf 109 39 , , , cord-008590-xivnsldf 109 40 103 103 CD cord-008590-xivnsldf 109 41 ) ) -RRB- cord-008590-xivnsldf 109 42 . . . cord-008590-xivnsldf 110 1 Experiments experiment NNS cord-008590-xivnsldf 110 2 on on IN cord-008590-xivnsldf 110 3 the the DT cord-008590-xivnsldf 110 4 influence influence NN cord-008590-xivnsldf 110 5 of of IN cord-008590-xivnsldf 110 6 colchicine colchicine NN cord-008590-xivnsldf 110 7 on on IN cord-008590-xivnsldf 110 8 N n CD cord-008590-xivnsldf 110 9 - - HYPH cord-008590-xivnsldf 110 10 linked link VBN cord-008590-xivnsldf 110 11 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 110 12 maturation maturation NN cord-008590-xivnsldf 110 13 of of IN cord-008590-xivnsldf 110 14 secretory secretory JJ cord-008590-xivnsldf 110 15 glycoproteins glycoprotein NNS cord-008590-xivnsldf 110 16 show show VBP cord-008590-xivnsldf 110 17 that that IN cord-008590-xivnsldf 110 18 it -PRON- PRP cord-008590-xivnsldf 110 19 interrupts interrupt VBZ cord-008590-xivnsldf 110 20 ICT ICT NNP cord-008590-xivnsldf 110 21 at at IN cord-008590-xivnsldf 110 22 a a DT cord-008590-xivnsldf 110 23 very very RB cord-008590-xivnsldf 110 24 late late JJ cord-008590-xivnsldf 110 25 site site NN cord-008590-xivnsldf 110 26 ( ( -LRB- cord-008590-xivnsldf 110 27 102 102 CD cord-008590-xivnsldf 110 28 , , , cord-008590-xivnsldf 110 29 103 103 CD cord-008590-xivnsldf 110 30 ) ) -RRB- cord-008590-xivnsldf 110 31 . . . cord-008590-xivnsldf 111 1 It -PRON- PRP cord-008590-xivnsldf 111 2 is be VBZ cord-008590-xivnsldf 111 3 therefore therefore RB cord-008590-xivnsldf 111 4 not not RB cord-008590-xivnsldf 111 5 surprising surprising JJ cord-008590-xivnsldf 111 6 that that IN cord-008590-xivnsldf 111 7 it -PRON- PRP cord-008590-xivnsldf 111 8 causes cause VBZ cord-008590-xivnsldf 111 9 an an DT cord-008590-xivnsldf 111 10 accumulation accumulation NN cord-008590-xivnsldf 111 11 of of IN cord-008590-xivnsldf 111 12 postcisternal postcisternal JJ cord-008590-xivnsldf 111 13 elements element NNS cord-008590-xivnsldf 111 14 containing contain VBG cord-008590-xivnsldf 111 15 an an DT cord-008590-xivnsldf 111 16 excess excess NN cord-008590-xivnsldf 111 17 of of IN cord-008590-xivnsldf 111 18 both both DT cord-008590-xivnsldf 111 19 albumin albumin NN cord-008590-xivnsldf 111 20 and and CC cord-008590-xivnsldf 111 21 proalbumin proalbumin NNP cord-008590-xivnsldf 111 22 ( ( -LRB- cord-008590-xivnsldf 111 23 102 102 CD cord-008590-xivnsldf 111 24 , , , cord-008590-xivnsldf 111 25 103 103 CD cord-008590-xivnsldf 111 26 ) ) -RRB- cord-008590-xivnsldf 111 27 . . . cord-008590-xivnsldf 112 1 When when WRB cord-008590-xivnsldf 112 2 taxol taxol NNP cord-008590-xivnsldf 112 3 is be VBZ cord-008590-xivnsldf 112 4 used use VBN cord-008590-xivnsldf 112 5 to to TO cord-008590-xivnsldf 112 6 stabilize stabilize VB cord-008590-xivnsldf 112 7 microtubules microtubule NNS cord-008590-xivnsldf 112 8 cleavage cleavage NN cord-008590-xivnsldf 112 9 to to IN cord-008590-xivnsldf 112 10 albumin albumin NN cord-008590-xivnsldf 112 11 proceeds proceed NNS cord-008590-xivnsldf 112 12 , , , cord-008590-xivnsldf 112 13 and and CC cord-008590-xivnsldf 112 14 discharge discharge NN cord-008590-xivnsldf 112 15 is be VBZ cord-008590-xivnsldf 112 16 much much RB cord-008590-xivnsldf 112 17 reduced reduce VBN cord-008590-xivnsldf 112 18 ( ( -LRB- cord-008590-xivnsldf 112 19 94 94 CD cord-008590-xivnsldf 112 20 ) ) -RRB- cord-008590-xivnsldf 112 21 . . . cord-008590-xivnsldf 113 1 In in IN cord-008590-xivnsldf 113 2 the the DT cord-008590-xivnsldf 113 3 case case NN cord-008590-xivnsldf 113 4 of of IN cord-008590-xivnsldf 113 5 glycoproteins glycoprotein NNS cord-008590-xivnsldf 113 6 which which WDT cord-008590-xivnsldf 113 7 undergo undergo VBP cord-008590-xivnsldf 113 8 proteolytic proteolytic JJ cord-008590-xivnsldf 113 9 cleavage cleavage NN cord-008590-xivnsldf 113 10 prior prior RB cord-008590-xivnsldf 113 11 to to IN cord-008590-xivnsldf 113 12 arrival arrival NN cord-008590-xivnsldf 113 13 at at IN cord-008590-xivnsldf 113 14 the the DT cord-008590-xivnsldf 113 15 cell cell NN cord-008590-xivnsldf 113 16 surface surface NN cord-008590-xivnsldf 113 17 there there EX cord-008590-xivnsldf 113 18 are be VBP cord-008590-xivnsldf 113 19 insufficient insufficient JJ cord-008590-xivnsldf 113 20 data datum NNS cord-008590-xivnsldf 113 21 to to TO cord-008590-xivnsldf 113 22 generalize generalize VB cord-008590-xivnsldf 113 23 with with IN cord-008590-xivnsldf 113 24 respect respect NN cord-008590-xivnsldf 113 25 to to IN cord-008590-xivnsldf 113 26 the the DT cord-008590-xivnsldf 113 27 relative relative JJ cord-008590-xivnsldf 113 28 order order NN cord-008590-xivnsldf 113 29 of of IN cord-008590-xivnsldf 113 30 the the DT cord-008590-xivnsldf 113 31 events event NNS cord-008590-xivnsldf 113 32 of of IN cord-008590-xivnsldf 113 33 carbohydrate carbohydrate NN cord-008590-xivnsldf 113 34 maturation maturation NN cord-008590-xivnsldf 113 35 and and CC cord-008590-xivnsldf 113 36 proteolysis proteolysis NN cord-008590-xivnsldf 113 37 . . . cord-008590-xivnsldf 114 1 Nevertheless nevertheless RB cord-008590-xivnsldf 114 2 , , , cord-008590-xivnsldf 114 3 pulse pulse NN cord-008590-xivnsldf 114 4 - - HYPH cord-008590-xivnsldf 114 5 chase chase NN cord-008590-xivnsldf 114 6 studies study NNS cord-008590-xivnsldf 114 7 of of IN cord-008590-xivnsldf 114 8 proopiomelanocortin proopiomelanocortin NNP cord-008590-xivnsldf 114 9 biosynthesis biosynthesis NN cord-008590-xivnsldf 114 10 employing employ VBG cord-008590-xivnsldf 114 11 monensin monensin NNP cord-008590-xivnsldf 114 12 suggest suggest VBP cord-008590-xivnsldf 114 13 that that IN cord-008590-xivnsldf 114 14 the the DT cord-008590-xivnsldf 114 15 acquisition acquisition NN cord-008590-xivnsldf 114 16 of of IN cord-008590-xivnsldf 114 17 mature mature JJ cord-008590-xivnsldf 114 18 carbohydrate carbohydrate NN cord-008590-xivnsldf 114 19 units unit NNS cord-008590-xivnsldf 114 20 precedes precede VBZ cord-008590-xivnsldf 114 21 proteolysis proteolysis NN cord-008590-xivnsldf 114 22 ( ( -LRB- cord-008590-xivnsldf 114 23 17 17 CD cord-008590-xivnsldf 114 24 ) ) -RRB- cord-008590-xivnsldf 114 25 . . . cord-008590-xivnsldf 115 1 In in IN cord-008590-xivnsldf 115 2 this this DT cord-008590-xivnsldf 115 3 study study NN cord-008590-xivnsldf 115 4 the the DT cord-008590-xivnsldf 115 5 inhibition inhibition NN cord-008590-xivnsldf 115 6 by by IN cord-008590-xivnsldf 115 7 monensin monensin NNP cord-008590-xivnsldf 115 8 may may MD cord-008590-xivnsldf 115 9 reflect reflect VB cord-008590-xivnsldf 115 10 neutralization neutralization NN cord-008590-xivnsldf 115 11 of of IN cord-008590-xivnsldf 115 12 intragranular intragranular JJ cord-008590-xivnsldf 115 13 pH ph NN cord-008590-xivnsldf 115 14 since since IN cord-008590-xivnsldf 115 15 the the DT cord-008590-xivnsldf 115 16 granule granule NN cord-008590-xivnsldf 115 17 protease protease NN cord-008590-xivnsldf 115 18 has have VBZ cord-008590-xivnsldf 115 19 a a DT cord-008590-xivnsldf 115 20 pH ph NN cord-008590-xivnsldf 115 21 optimum optimum NN cord-008590-xivnsldf 115 22 at at IN cord-008590-xivnsldf 115 23 pH pH NNP cord-008590-xivnsldf 115 24 5 5 CD cord-008590-xivnsldf 115 25 ( ( -LRB- cord-008590-xivnsldf 115 26 69 69 CD cord-008590-xivnsldf 115 27 ) ) -RRB- cord-008590-xivnsldf 115 28 . . . cord-008590-xivnsldf 116 1 Chondroitin chondroitin NN cord-008590-xivnsldf 116 2 sulfate sulfate NN cord-008590-xivnsldf 116 3 , , , cord-008590-xivnsldf 116 4 dermatan dermatan NNP cord-008590-xivnsldf 116 5 sulfate sulfate NN cord-008590-xivnsldf 116 6 , , , cord-008590-xivnsldf 116 7 heparin heparin NN cord-008590-xivnsldf 116 8 , , , cord-008590-xivnsldf 116 9 and and CC cord-008590-xivnsldf 116 10 heraran heraran NN cord-008590-xivnsldf 116 11 sulfate sulfate NN cord-008590-xivnsldf 116 12 all all DT cord-008590-xivnsldf 116 13 contain contain VBP cord-008590-xivnsldf 116 14 the the DT cord-008590-xivnsldf 116 15 same same JJ cord-008590-xivnsldf 116 16 internal internal JJ cord-008590-xivnsldf 116 17 tetrasaccharide tetrasaccharide NN cord-008590-xivnsldf 116 18 structure structure NN cord-008590-xivnsldf 116 19 ( ( -LRB- cord-008590-xivnsldf 116 20 Xyl Xyl NNP cord-008590-xivnsldf 116 21 - - HYPH cord-008590-xivnsldf 116 22 Gal Gal NNP cord-008590-xivnsldf 116 23 - - HYPH cord-008590-xivnsldf 116 24 Gal gal NN cord-008590-xivnsldf 116 25 - - HYPH cord-008590-xivnsldf 116 26 GlcUA glcua NN cord-008590-xivnsldf 116 27 ) ) -RRB- cord-008590-xivnsldf 116 28 linked link VBN cord-008590-xivnsldf 116 29 to to IN cord-008590-xivnsldf 116 30 multiple multiple JJ cord-008590-xivnsldf 116 31 serine serine NN cord-008590-xivnsldf 116 32 residues residue NNS cord-008590-xivnsldf 116 33 of of IN cord-008590-xivnsldf 116 34 a a DT cord-008590-xivnsldf 116 35 protein protein NN cord-008590-xivnsldf 116 36 core core NN cord-008590-xivnsldf 116 37 . . . cord-008590-xivnsldf 117 1 The the DT cord-008590-xivnsldf 117 2 four four CD cord-008590-xivnsldf 117 3 corresponding correspond VBG cord-008590-xivnsldf 117 4 monosaccharide monosaccharide NN cord-008590-xivnsldf 117 5 transferases transferase NNS cord-008590-xivnsldf 117 6 have have VBP cord-008590-xivnsldf 117 7 been be VBN cord-008590-xivnsldf 117 8 characterized characterize VBN cord-008590-xivnsldf 117 9 and and CC cord-008590-xivnsldf 117 10 substantially substantially RB cord-008590-xivnsldf 117 11 purified purify VBN cord-008590-xivnsldf 117 12 , , , cord-008590-xivnsldf 117 13 and and CC cord-008590-xivnsldf 117 14 a a DT cord-008590-xivnsldf 117 15 limited limited JJ cord-008590-xivnsldf 117 16 amount amount NN cord-008590-xivnsldf 117 17 of of IN cord-008590-xivnsldf 117 18 information information NN cord-008590-xivnsldf 117 19 is be VBZ cord-008590-xivnsldf 117 20 available available JJ cord-008590-xivnsldf 117 21 concerning concern VBG cord-008590-xivnsldf 117 22 the the DT cord-008590-xivnsldf 117 23 polypeptide polypeptide NN cord-008590-xivnsldf 117 24 recognized recognize VBN cord-008590-xivnsldf 117 25 by by IN cord-008590-xivnsldf 117 26 the the DT cord-008590-xivnsldf 117 27 initiator initiator NN cord-008590-xivnsldf 117 28 xylosyl xylosyl NNP cord-008590-xivnsldf 117 29 transferase transferase NNP cord-008590-xivnsldf 117 30 ( ( -LRB- cord-008590-xivnsldf 117 31 15 15 CD cord-008590-xivnsldf 117 32 ) ) -RRB- cord-008590-xivnsldf 117 33 . . . cord-008590-xivnsldf 118 1 There there EX cord-008590-xivnsldf 118 2 are be VBP cord-008590-xivnsldf 118 3 provisional provisional JJ cord-008590-xivnsldf 118 4 indications indication NNS cord-008590-xivnsldf 118 5 that that IN cord-008590-xivnsldf 118 6 at at IN cord-008590-xivnsldf 118 7 least least JJS cord-008590-xivnsldf 118 8 the the DT cord-008590-xivnsldf 118 9 xylosyl xylosyl NNP cord-008590-xivnsldf 118 10 and and CC cord-008590-xivnsldf 118 11 first first JJ cord-008590-xivnsldf 118 12 galactosyl galactosyl NNP cord-008590-xivnsldf 118 13 transferases transferase NNS cord-008590-xivnsldf 118 14 might may MD cord-008590-xivnsldf 118 15 be be VB cord-008590-xivnsldf 118 16 physically physically RB cord-008590-xivnsldf 118 17 associated associate VBN cord-008590-xivnsldf 118 18 with with IN cord-008590-xivnsldf 118 19 each each DT cord-008590-xivnsldf 118 20 other other JJ cord-008590-xivnsldf 118 21 - - : cord-008590-xivnsldf 118 22 they -PRON- PRP cord-008590-xivnsldf 118 23 are be VBP cord-008590-xivnsldf 118 24 both both DT cord-008590-xivnsldf 118 25 recovered recover VBN cord-008590-xivnsldf 118 26 in in IN cord-008590-xivnsldf 118 27 immunoprecipitates immunoprecipitate NNS cord-008590-xivnsldf 118 28 prepared prepare VBN cord-008590-xivnsldf 118 29 with with IN cord-008590-xivnsldf 118 30 monospecific monospecific JJ cord-008590-xivnsldf 118 31 anti anti JJ cord-008590-xivnsldf 118 32 - - JJ cord-008590-xivnsldf 118 33 xylosyl xylosyl JJ cord-008590-xivnsldf 118 34 transferase transferase NNP cord-008590-xivnsldf 118 35 antisera antisera NNP cord-008590-xivnsldf 118 36 ( ( -LRB- cord-008590-xivnsldf 118 37 12 12 CD cord-008590-xivnsldf 118 38 I i NN cord-008590-xivnsldf 118 39 ) ) -RRB- cord-008590-xivnsldf 118 40 . . . cord-008590-xivnsldf 119 1 The the DT cord-008590-xivnsldf 119 2 literature literature NN cord-008590-xivnsldf 119 3 is be VBZ cord-008590-xivnsldf 119 4 equivocal equivocal JJ cord-008590-xivnsldf 119 5 with with IN cord-008590-xivnsldf 119 6 respect respect NN cord-008590-xivnsldf 119 7 to to IN cord-008590-xivnsldf 119 8 the the DT cord-008590-xivnsldf 119 9 cytologic cytologic NNP cord-008590-xivnsldf 119 10 site(s site(s NNP cord-008590-xivnsldf 119 11 ) ) -RRB- cord-008590-xivnsldf 119 12 of of IN cord-008590-xivnsldf 119 13 addition addition NN cord-008590-xivnsldf 119 14 of of IN cord-008590-xivnsldf 119 15 these these DT cord-008590-xivnsldf 119 16 internal internal JJ cord-008590-xivnsldf 119 17 sugar sugar NN cord-008590-xivnsldf 119 18 residues residue NNS cord-008590-xivnsldf 119 19 . . . cord-008590-xivnsldf 120 1 Attempts attempt NNS cord-008590-xivnsldf 120 2 at at IN cord-008590-xivnsldf 120 3 subcellular subcellular JJ cord-008590-xivnsldf 120 4 fractionation fractionation NN cord-008590-xivnsldf 120 5 of of IN cord-008590-xivnsldf 120 6 embryonic embryonic JJ cord-008590-xivnsldf 120 7 cartilage cartilage NN cord-008590-xivnsldf 120 8 suggest suggest VBP cord-008590-xivnsldf 120 9 that that IN cord-008590-xivnsldf 120 10 the the DT cord-008590-xivnsldf 120 11 four four CD cord-008590-xivnsldf 120 12 corresponding correspond VBG cord-008590-xivnsldf 120 13 enzyme enzyme NN cord-008590-xivnsldf 120 14 activities activity NNS cord-008590-xivnsldf 120 15 belong belong VBP cord-008590-xivnsldf 120 16 to to IN cord-008590-xivnsldf 120 17 both both CC cord-008590-xivnsldf 120 18 rough rough JJ cord-008590-xivnsldf 120 19 and and CC cord-008590-xivnsldf 120 20 smooth smooth JJ cord-008590-xivnsldf 120 21 microsomal microsomal JJ cord-008590-xivnsldf 120 22 fractions fraction NNS cord-008590-xivnsldf 120 23 ; ; : cord-008590-xivnsldf 120 24 however however RB cord-008590-xivnsldf 120 25 , , , cord-008590-xivnsldf 120 26 the the DT cord-008590-xivnsldf 120 27 enzyme enzyme NN cord-008590-xivnsldf 120 28 assays assay NNS cord-008590-xivnsldf 120 29 employed employ VBN cord-008590-xivnsldf 120 30 are be VBP cord-008590-xivnsldf 120 31 in in IN cord-008590-xivnsldf 120 32 several several JJ cord-008590-xivnsldf 120 33 respects respect NNS cord-008590-xivnsldf 120 34 not not RB cord-008590-xivnsldf 120 35 ideal ideal JJ cord-008590-xivnsldf 120 36 ( ( -LRB- cord-008590-xivnsldf 120 37 51 51 CD cord-008590-xivnsldf 120 38 ) ) -RRB- cord-008590-xivnsldf 120 39 . . . cord-008590-xivnsldf 121 1 Part part NN cord-008590-xivnsldf 121 2 of of IN cord-008590-xivnsldf 121 3 the the DT cord-008590-xivnsldf 121 4 fascination fascination NN cord-008590-xivnsldf 121 5 with with IN cord-008590-xivnsldf 121 6 proteoglycan proteoglycan JJ cord-008590-xivnsldf 121 7 biosynthesis biosynthesis NN cord-008590-xivnsldf 121 8 comes come VBZ cord-008590-xivnsldf 121 9 from from IN cord-008590-xivnsldf 121 10 recognition recognition NN cord-008590-xivnsldf 121 11 of of IN cord-008590-xivnsldf 121 12 their -PRON- PRP$ cord-008590-xivnsldf 121 13 immense immense JJ cord-008590-xivnsldf 121 14 size size NN cord-008590-xivnsldf 121 15 [ [ -LRB- cord-008590-xivnsldf 121 16 beautifully beautifully RB cord-008590-xivnsldf 121 17 visualized visualize VBN cord-008590-xivnsldf 121 18 by by IN cord-008590-xivnsldf 121 19 electron electron NN cord-008590-xivnsldf 121 20 microscopy microscopy NNS cord-008590-xivnsldf 121 21 ( ( -LRB- cord-008590-xivnsldf 121 22 62 62 CD cord-008590-xivnsldf 121 23 ) ) -RRB- cord-008590-xivnsldf 121 24 ] ] -RRB- cord-008590-xivnsldf 121 25 relative relative JJ cord-008590-xivnsldf 121 26 to to IN cord-008590-xivnsldf 121 27 the the DT cord-008590-xivnsldf 121 28 dimensions dimension NNS cord-008590-xivnsldf 121 29 of of IN cord-008590-xivnsldf 121 30 the the DT cord-008590-xivnsldf 121 31 machinery machinery NN cord-008590-xivnsldf 121 32 of of IN cord-008590-xivnsldf 121 33 ICT ICT NNP cord-008590-xivnsldf 121 34 . . . cord-008590-xivnsldf 122 1 A a DT cord-008590-xivnsldf 122 2 monomer monomer NN cord-008590-xivnsldf 122 3 of of IN cord-008590-xivnsldf 122 4 cartilage cartilage NN cord-008590-xivnsldf 122 5 proteoglycan proteoglycan NN cord-008590-xivnsldf 122 6 , , , cord-008590-xivnsldf 122 7 for for IN cord-008590-xivnsldf 122 8 example example NN cord-008590-xivnsldf 122 9 , , , cord-008590-xivnsldf 122 10 consists consist VBZ cord-008590-xivnsldf 122 11 of of IN cord-008590-xivnsldf 122 12 a a DT cord-008590-xivnsldf 122 13 core core NN cord-008590-xivnsldf 122 14 protein protein NN cord-008590-xivnsldf 122 15 ( ( -LRB- cord-008590-xivnsldf 122 16 -300 -300 NNP cord-008590-xivnsldf 122 17 nm nm RB cord-008590-xivnsldf 122 18 long long RB cord-008590-xivnsldf 122 19 when when WRB cord-008590-xivnsldf 122 20 spread spread NN cord-008590-xivnsldf 122 21 ) ) -RRB- cord-008590-xivnsldf 122 22 which which WDT cord-008590-xivnsldf 122 23 bears bear VBZ cord-008590-xivnsldf 122 24 on on IN cord-008590-xivnsldf 122 25 the the DT cord-008590-xivnsldf 122 26 order order NN cord-008590-xivnsldf 122 27 of of IN cord-008590-xivnsldf 122 28 80 80 CD cord-008590-xivnsldf 122 29 lateral lateral JJ cord-008590-xivnsldf 122 30 sulfated sulfated JJ cord-008590-xivnsldf 122 31 chains chain NNS cord-008590-xivnsldf 122 32 ( ( -LRB- cord-008590-xivnsldf 122 33 internal internal JJ cord-008590-xivnsldf 122 34 tetrasaccharide tetrasaccharide NN cord-008590-xivnsldf 122 35 and and CC cord-008590-xivnsldf 122 36 repeating repeat VBG cord-008590-xivnsldf 122 37 sulfated sulfate VBN cord-008590-xivnsldf 122 38 GalNAc galnac NN cord-008590-xivnsldf 122 39 , , , cord-008590-xivnsldf 122 40 GlcUA GlcUA NNP cord-008590-xivnsldf 122 41 disaccharide disaccharide NN cord-008590-xivnsldf 122 42 ) ) -RRB- cord-008590-xivnsldf 122 43 each each DT cord-008590-xivnsldf 122 44 of of IN cord-008590-xivnsldf 122 45 which which WDT cord-008590-xivnsldf 122 46 is be VBZ cord-008590-xivnsldf 122 47 about about RB cord-008590-xivnsldf 122 48 100 100 CD cord-008590-xivnsldf 122 49 residues residue NNS cord-008590-xivnsldf 122 50 long long RB cord-008590-xivnsldf 122 51 . . . cord-008590-xivnsldf 123 1 Recent recent JJ cord-008590-xivnsldf 123 2 experiments experiment NNS cord-008590-xivnsldf 123 3 making make VBG cord-008590-xivnsldf 123 4 use use NN cord-008590-xivnsldf 123 5 of of IN cord-008590-xivnsldf 123 6 antisera antisera NN cord-008590-xivnsldf 123 7 directed direct VBN cord-008590-xivnsldf 123 8 against against IN cord-008590-xivnsldf 123 9 protein protein NN cord-008590-xivnsldf 123 10 determinants determinant NNS cord-008590-xivnsldf 123 11 of of IN cord-008590-xivnsldf 123 12 the the DT cord-008590-xivnsldf 123 13 proteoglycan proteoglycan NN cord-008590-xivnsldf 123 14 monomer monomer NN cord-008590-xivnsldf 123 15 indicate indicate VBP cord-008590-xivnsldf 123 16 that that IN cord-008590-xivnsldf 123 17 there there EX cord-008590-xivnsldf 123 18 is be VBZ cord-008590-xivnsldf 123 19 a a DT cord-008590-xivnsldf 123 20 substantial substantial JJ cord-008590-xivnsldf 123 21 intracellular intracellular JJ cord-008590-xivnsldf 123 22 pool pool NN cord-008590-xivnsldf 123 23 of of IN cord-008590-xivnsldf 123 24 core core NN cord-008590-xivnsldf 123 25 protein protein NN cord-008590-xivnsldf 123 26 lacking lack VBG cord-008590-xivnsldf 123 27 the the DT cord-008590-xivnsldf 123 28 repeating repeat VBG cord-008590-xivnsldf 123 29 disaccharides disaccharide NNS cord-008590-xivnsldf 123 30 ( ( -LRB- cord-008590-xivnsldf 123 31 12 12 CD cord-008590-xivnsldf 123 32 , , , cord-008590-xivnsldf 123 33 65 65 CD cord-008590-xivnsldf 123 34 ) ) -RRB- cord-008590-xivnsldf 123 35 . . . cord-008590-xivnsldf 124 1 In in IN cord-008590-xivnsldf 124 2 amino amino NN cord-008590-xivnsldf 124 3 acid acid NN cord-008590-xivnsldf 124 4 pulse pulse NN cord-008590-xivnsldf 124 5 - - HYPH cord-008590-xivnsldf 124 6 chase chase NN cord-008590-xivnsldf 124 7 experiments experiment NNS cord-008590-xivnsldf 124 8 , , , cord-008590-xivnsldf 124 9 this this DT cord-008590-xivnsldf 124 10 pool pool NN cord-008590-xivnsldf 124 11 , , , cord-008590-xivnsldf 124 12 which which WDT cord-008590-xivnsldf 124 13 is be VBZ cord-008590-xivnsldf 124 14 presumably presumably RB cord-008590-xivnsldf 124 15 in in IN cord-008590-xivnsldf 124 16 the the DT cord-008590-xivnsldf 124 17 RER rer NN cord-008590-xivnsldf 124 18 , , , cord-008590-xivnsldf 124 19 can can MD cord-008590-xivnsldf 124 20 be be VB cord-008590-xivnsldf 124 21 chased chase VBN cord-008590-xivnsldf 124 22 into into IN cord-008590-xivnsldf 124 23 mature mature JJ cord-008590-xivnsldf 124 24 proteoglycan proteoglycan NN cord-008590-xivnsldf 124 25 with with IN cord-008590-xivnsldf 124 26 a a DT cord-008590-xivnsldf 124 27 half half JJ cord-008590-xivnsldf 124 28 - - HYPH cord-008590-xivnsldf 124 29 time time NN cord-008590-xivnsldf 124 30 of of IN cord-008590-xivnsldf 124 31 -80 -80 NNP cord-008590-xivnsldf 124 32 minutes minute NNS cord-008590-xivnsldf 124 33 , , , cord-008590-xivnsldf 124 34 i.e. i.e. FW cord-008590-xivnsldf 124 35 , , , cord-008590-xivnsldf 124 36 Several several JJ cord-008590-xivnsldf 124 37 noncovalent noncovalent NNP cord-008590-xivnsldf 124 38 assembly assembly NNP cord-008590-xivnsldf 124 39 steps step NNS cord-008590-xivnsldf 124 40 result result VBP cord-008590-xivnsldf 124 41 in in IN cord-008590-xivnsldf 124 42 the the DT cord-008590-xivnsldf 124 43 ultimate ultimate JJ cord-008590-xivnsldf 124 44 extracellular extracellular JJ cord-008590-xivnsldf 124 45 product product NN cord-008590-xivnsldf 124 46 : : : cord-008590-xivnsldf 124 47 monomeric monomeric JJ cord-008590-xivnsldf 124 48 units unit NNS cord-008590-xivnsldf 124 49 of of IN cord-008590-xivnsldf 124 50 derivatized derivatized JJ cord-008590-xivnsldf 124 51 core core NN cord-008590-xivnsldf 124 52 protein protein NN cord-008590-xivnsldf 124 53 firmly firmly RB cord-008590-xivnsldf 124 54 associated associate VBD cord-008590-xivnsldf 124 55 with with IN cord-008590-xivnsldf 124 56 the the DT cord-008590-xivnsldf 124 57 hydrophobic hydrophobic JJ cord-008590-xivnsldf 124 58 " " `` cord-008590-xivnsldf 124 59 link link NN cord-008590-xivnsldf 124 60 " " '' cord-008590-xivnsldf 124 61 glycoprotein glycoprotein NN cord-008590-xivnsldf 124 62 and and CC cord-008590-xivnsldf 124 63 hyaluronic hyaluronic NN cord-008590-xivnsldf 124 64 acid acid NN cord-008590-xivnsldf 124 65 . . . cord-008590-xivnsldf 125 1 A a DT cord-008590-xivnsldf 125 2 set set NN cord-008590-xivnsldf 125 3 of of IN cord-008590-xivnsldf 125 4 ingenious ingenious JJ cord-008590-xivnsldf 125 5 competition competition NN cord-008590-xivnsldf 125 6 experiments experiment NNS cord-008590-xivnsldf 125 7 has have VBZ cord-008590-xivnsldf 125 8 shown show VBN cord-008590-xivnsldf 125 9 that that IN cord-008590-xivnsldf 125 10 hyaluronic hyaluronic NN cord-008590-xivnsldf 125 11 acid acid NN cord-008590-xivnsldf 125 12 - - : cord-008590-xivnsldf 125 13 which which WDT cord-008590-xivnsldf 125 14 is be VBZ cord-008590-xivnsldf 125 15 synthesized synthesize VBN cord-008590-xivnsldf 125 16 by by IN cord-008590-xivnsldf 125 17 the the DT cord-008590-xivnsldf 125 18 same same JJ cord-008590-xivnsldf 125 19 cells cell NNS cord-008590-xivnsldf 125 20 which which WDT cord-008590-xivnsldf 125 21 synthesize synthesize VBP cord-008590-xivnsldf 125 22 the the DT cord-008590-xivnsldf 125 23 monomer monomer NN cord-008590-xivnsldf 125 24 - - : cord-008590-xivnsldf 125 25 is be VBZ cord-008590-xivnsldf 125 26 bound bind VBN cord-008590-xivnsldf 125 27 only only RB cord-008590-xivnsldf 125 28 after after IN cord-008590-xivnsldf 125 29 discharge discharge NN cord-008590-xivnsldf 125 30 ( ( -LRB- cord-008590-xivnsldf 125 31 63 63 CD cord-008590-xivnsldf 125 32 ) ) -RRB- cord-008590-xivnsldf 125 33 . . . cord-008590-xivnsldf 126 1 Although although IN cord-008590-xivnsldf 126 2 intracellular intracellular JJ cord-008590-xivnsldf 126 3 monomer monomer NN cord-008590-xivnsldf 126 4 can can MD cord-008590-xivnsldf 126 5 bind bind VB cord-008590-xivnsldf 126 6 the the DT cord-008590-xivnsldf 126 7 link link NN cord-008590-xivnsldf 126 8 protein protein NN cord-008590-xivnsldf 126 9 after after IN cord-008590-xivnsldf 126 10 solubilization solubilization NN cord-008590-xivnsldf 126 11 , , , cord-008590-xivnsldf 126 12 it -PRON- PRP cord-008590-xivnsldf 126 13 is be VBZ cord-008590-xivnsldf 126 14 not not RB cord-008590-xivnsldf 126 15 known know VBN cord-008590-xivnsldf 126 16 whether whether IN cord-008590-xivnsldf 126 17 this this DT cord-008590-xivnsldf 126 18 event event NN cord-008590-xivnsldf 126 19 or or CC cord-008590-xivnsldf 126 20 the the DT cord-008590-xivnsldf 126 21 association association NN cord-008590-xivnsldf 126 22 with with IN cord-008590-xivnsldf 126 23 collagen collagen NNP cord-008590-xivnsldf 126 24 ( ( -LRB- cord-008590-xivnsldf 126 25 121 121 CD cord-008590-xivnsldf 126 26 , , , cord-008590-xivnsldf 126 27 143 143 CD cord-008590-xivnsldf 126 28 ) ) -RRB- cord-008590-xivnsldf 126 29 normally normally RB cord-008590-xivnsldf 126 30 occurs occur VBZ cord-008590-xivnsldf 126 31 prior prior RB cord-008590-xivnsldf 126 32 to to IN cord-008590-xivnsldf 126 33 discharge discharge NN cord-008590-xivnsldf 126 34 . . . cord-008590-xivnsldf 127 1 A a DT cord-008590-xivnsldf 127 2 word word NN cord-008590-xivnsldf 127 3 should should MD cord-008590-xivnsldf 127 4 be be VB cord-008590-xivnsldf 127 5 said say VBN cord-008590-xivnsldf 127 6 about about IN cord-008590-xivnsldf 127 7 the the DT cord-008590-xivnsldf 127 8 biosynthesis biosynthesis NN cord-008590-xivnsldf 127 9 of of IN cord-008590-xivnsldf 127 10 hyaluronic hyaluronic NN cord-008590-xivnsldf 127 11 acid acid NN cord-008590-xivnsldf 127 12 . . . cord-008590-xivnsldf 128 1 It -PRON- PRP cord-008590-xivnsldf 128 2 is be VBZ cord-008590-xivnsldf 128 3 at at IN cord-008590-xivnsldf 128 4 no no DT cord-008590-xivnsldf 128 5 time time NN cord-008590-xivnsldf 128 6 covalently covalently RB cord-008590-xivnsldf 128 7 bound bind VBN cord-008590-xivnsldf 128 8 to to IN cord-008590-xivnsldf 128 9 protein protein NN cord-008590-xivnsldf 128 10 and and CC cord-008590-xivnsldf 128 11 cycloheximide cycloheximide NN cord-008590-xivnsldf 128 12 does do VBZ cord-008590-xivnsldf 128 13 not not RB cord-008590-xivnsldf 128 14 interrupt interrupt VB cord-008590-xivnsldf 128 15 its -PRON- PRP$ cord-008590-xivnsldf 128 16 biosynthesis biosynthesis NN cord-008590-xivnsldf 128 17 ( ( -LRB- cord-008590-xivnsldf 128 18 80 80 CD cord-008590-xivnsldf 128 19 ) ) -RRB- cord-008590-xivnsldf 128 20 . . . cord-008590-xivnsldf 129 1 Its -PRON- PRP$ cord-008590-xivnsldf 129 2 repeating repeat VBG cord-008590-xivnsldf 129 3 disaccharide disaccharide NN cord-008590-xivnsldf 129 4 ( ( -LRB- cord-008590-xivnsldf 129 5 GlcNAc GlcNAc NNP cord-008590-xivnsldf 129 6 , , , cord-008590-xivnsldf 129 7 GlcUA GlcUA NNP cord-008590-xivnsldf 129 8 ) ) -RRB- cord-008590-xivnsldf 129 9 can can MD cord-008590-xivnsldf 129 10 be be VB cord-008590-xivnsldf 129 11 assembled assemble VBN cord-008590-xivnsldf 129 12 in in IN cord-008590-xivnsldf 129 13 vitro vitro FW cord-008590-xivnsldf 129 14 by by IN cord-008590-xivnsldf 129 15 incubating incubate VBG cord-008590-xivnsldf 129 16 cell cell NN cord-008590-xivnsldf 129 17 extracts extract NNS cord-008590-xivnsldf 129 18 with with IN cord-008590-xivnsldf 129 19 UDP udp NN cord-008590-xivnsldf 129 20 - - HYPH cord-008590-xivnsldf 129 21 activated activate VBN cord-008590-xivnsldf 129 22 substrates substrate NNS cord-008590-xivnsldf 129 23 ( ( -LRB- cord-008590-xivnsldf 129 24 2 2 CD cord-008590-xivnsldf 129 25 ) ) -RRB- cord-008590-xivnsldf 129 26 . . . cord-008590-xivnsldf 130 1 Growth growth NN cord-008590-xivnsldf 130 2 is be VBZ cord-008590-xivnsldf 130 3 at at IN cord-008590-xivnsldf 130 4 the the DT cord-008590-xivnsldf 130 5 reducing reduce VBG cord-008590-xivnsldf 130 6 end end NN cord-008590-xivnsldf 130 7 of of IN cord-008590-xivnsldf 130 8 the the DT cord-008590-xivnsldf 130 9 chain chain NN cord-008590-xivnsldf 130 10 , , , cord-008590-xivnsldf 130 11 which which WDT cord-008590-xivnsldf 130 12 is be VBZ cord-008590-xivnsldf 130 13 transiently transiently RB cord-008590-xivnsldf 130 14 membrane membrane NN cord-008590-xivnsldf 130 15 bound bind VBN cord-008590-xivnsldf 130 16 , , , cord-008590-xivnsldf 130 17 possibly possibly RB cord-008590-xivnsldf 130 18 via via IN cord-008590-xivnsldf 130 19 UDP udp NN cord-008590-xivnsldf 130 20 ( ( -LRB- cord-008590-xivnsldf 130 21 100b 100b NNP cord-008590-xivnsldf 130 22 ) ) -RRB- cord-008590-xivnsldf 130 23 . . . cord-008590-xivnsldf 131 1 Autoradiographic Autoradiographic NNP cord-008590-xivnsldf 131 2 study study NN cord-008590-xivnsldf 131 3 of of IN cord-008590-xivnsldf 131 4 synovial synovial JJ cord-008590-xivnsldf 131 5 cells cell NNS cord-008590-xivnsldf 131 6 proves prove VBZ cord-008590-xivnsldf 131 7 that that IN cord-008590-xivnsldf 131 8 hyaluronic hyaluronic NN cord-008590-xivnsldf 131 9 acid acid NN cord-008590-xivnsldf 131 10 synthesis synthesis NN cord-008590-xivnsldf 131 11 does do VBZ cord-008590-xivnsldf 131 12 indeed indeed RB cord-008590-xivnsldf 131 13 occur occur VB cord-008590-xivnsldf 131 14 within within IN cord-008590-xivnsldf 131 15 the the DT cord-008590-xivnsldf 131 16 GC GC NNP cord-008590-xivnsldf 131 17 ( ( -LRB- cord-008590-xivnsldf 131 18 5 5 CD cord-008590-xivnsldf 131 19 ) ) -RRB- cord-008590-xivnsldf 131 20 . . . cord-008590-xivnsldf 132 1 The the DT cord-008590-xivnsldf 132 2 demonstration demonstration NN cord-008590-xivnsldf 132 3 that that WDT cord-008590-xivnsldf 132 4 its -PRON- PRP$ cord-008590-xivnsldf 132 5 synthesis synthesis NN cord-008590-xivnsldf 132 6 is be VBZ cord-008590-xivnsldf 132 7 not not RB cord-008590-xivnsldf 132 8 influenced influence VBN cord-008590-xivnsldf 132 9 by by IN cord-008590-xivnsldf 132 10 doses dose NNS cord-008590-xivnsldf 132 11 of of IN cord-008590-xivnsldf 132 12 monensin monensin NNP cord-008590-xivnsldf 132 13 which which WDT cord-008590-xivnsldf 132 14 block block VBP cord-008590-xivnsldf 132 15 sulfation sulfation NN cord-008590-xivnsldf 132 16 of of IN cord-008590-xivnsldf 132 17 chondroitin chondroitin NN cord-008590-xivnsldf 132 18 sulfate sulfate NN cord-008590-xivnsldf 132 19 by by IN cord-008590-xivnsldf 132 20 the the DT cord-008590-xivnsldf 132 21 same same JJ cord-008590-xivnsldf 132 22 cells cell NNS cord-008590-xivnsldf 132 23 is be VBZ cord-008590-xivnsldf 132 24 consistant consistant JJ cord-008590-xivnsldf 132 25 with with IN cord-008590-xivnsldf 132 26 its -PRON- PRP$ cord-008590-xivnsldf 132 27 not not RB cord-008590-xivnsldf 132 28 having have VBG cord-008590-xivnsldf 132 29 a a DT cord-008590-xivnsldf 132 30 protein protein NN cord-008590-xivnsldf 132 31 core core NN cord-008590-xivnsldf 132 32 - - HYPH cord-008590-xivnsldf 132 33 sugar sugar NN cord-008590-xivnsldf 132 34 addition addition NN cord-008590-xivnsldf 132 35 can can MD cord-008590-xivnsldf 132 36 occur occur VB cord-008590-xivnsldf 132 37 in in IN cord-008590-xivnsldf 132 38 situ situ NNS cord-008590-xivnsldf 132 39 without without IN cord-008590-xivnsldf 132 40 ongoing ongoing JJ cord-008590-xivnsldf 132 41 ICT ICT NNP cord-008590-xivnsldf 132 42 ( ( -LRB- cord-008590-xivnsldf 132 43 84 84 CD cord-008590-xivnsldf 132 44 ) ) -RRB- cord-008590-xivnsldf 132 45 . . . cord-008590-xivnsldf 133 1 In in IN cord-008590-xivnsldf 133 2 order order NN cord-008590-xivnsldf 133 3 to to TO cord-008590-xivnsldf 133 4 assign assign VB cord-008590-xivnsldf 133 5 its -PRON- PRP$ cord-008590-xivnsldf 133 6 synthesis synthesis NN cord-008590-xivnsldf 133 7 to to IN cord-008590-xivnsldf 133 8 a a DT cord-008590-xivnsldf 133 9 subcompartment subcompartment NN cord-008590-xivnsldf 133 10 it -PRON- PRP cord-008590-xivnsldf 133 11 will will MD cord-008590-xivnsldf 133 12 be be VB cord-008590-xivnsldf 133 13 important important JJ cord-008590-xivnsldf 133 14 to to TO cord-008590-xivnsldf 133 15 learn learn VB cord-008590-xivnsldf 133 16 whether whether IN cord-008590-xivnsldf 133 17 monensin monensin NNP cord-008590-xivnsldf 133 18 blocks block VBZ cord-008590-xivnsldf 133 19 its -PRON- PRP$ cord-008590-xivnsldf 133 20 secretion secretion NN cord-008590-xivnsldf 133 21 . . . cord-008590-xivnsldf 134 1 The the DT cord-008590-xivnsldf 134 2 most most RBS cord-008590-xivnsldf 134 3 complex complex JJ cord-008590-xivnsldf 134 4 of of IN cord-008590-xivnsldf 134 5 the the DT cord-008590-xivnsldf 134 6 proteoglycan proteoglycan JJ cord-008590-xivnsldf 134 7 biosynthetic biosynthetic JJ cord-008590-xivnsldf 134 8 pathways pathway NNS cord-008590-xivnsldf 134 9 is be VBZ cord-008590-xivnsldf 134 10 that that DT cord-008590-xivnsldf 134 11 of of IN cord-008590-xivnsldf 134 12 heparin heparin NN cord-008590-xivnsldf 134 13 , , , cord-008590-xivnsldf 134 14 which which WDT cord-008590-xivnsldf 134 15 has have VBZ cord-008590-xivnsldf 134 16 been be VBN cord-008590-xivnsldf 134 17 in in IN cord-008590-xivnsldf 134 18 large large JJ cord-008590-xivnsldf 134 19 part part NN cord-008590-xivnsldf 134 20 elucidated elucidate VBN cord-008590-xivnsldf 134 21 with with IN cord-008590-xivnsldf 134 22 the the DT cord-008590-xivnsldf 134 23 help help NN cord-008590-xivnsldf 134 24 of of IN cord-008590-xivnsldf 134 25 mast mast NN cord-008590-xivnsldf 134 26 cell cell NN cord-008590-xivnsldf 134 27 tumors tumor NNS cord-008590-xivnsldf 134 28 . . . cord-008590-xivnsldf 135 1 Following follow VBG cord-008590-xivnsldf 135 2 the the DT cord-008590-xivnsldf 135 3 internal internal JJ cord-008590-xivnsldf 135 4 tetrasaccharide tetrasaccharide NN cord-008590-xivnsldf 135 5 addition addition NN cord-008590-xivnsldf 135 6 and and CC cord-008590-xivnsldf 135 7 elongation elongation NN cord-008590-xivnsldf 135 8 of of IN cord-008590-xivnsldf 135 9 the the DT cord-008590-xivnsldf 135 10 repeating repeating NN cord-008590-xivnsldf 135 11 ( ( -LRB- cord-008590-xivnsldf 135 12 GlcNAc GlcNAc NNP cord-008590-xivnsldf 135 13 , , , cord-008590-xivnsldf 135 14 GlcUA glcua NN cord-008590-xivnsldf 135 15 ) ) -RRB- cord-008590-xivnsldf 135 16 disaccharide disaccharide NN cord-008590-xivnsldf 135 17 units unit NNS cord-008590-xivnsldf 135 18 , , , cord-008590-xivnsldf 135 19 five five CD cord-008590-xivnsldf 135 20 further further JJ cord-008590-xivnsldf 135 21 enzymes enzyme NNS cord-008590-xivnsldf 135 22 intervene intervene VBP cord-008590-xivnsldf 135 23 ( ( -LRB- cord-008590-xivnsldf 135 24 76 76 CD cord-008590-xivnsldf 135 25 , , , cord-008590-xivnsldf 135 26 77 77 CD cord-008590-xivnsldf 135 27 ) ) -RRB- cord-008590-xivnsldf 135 28 : : : cord-008590-xivnsldf 135 29 ( ( -LRB- cord-008590-xivnsldf 135 30 1 1 LS cord-008590-xivnsldf 135 31 ) ) -RRB- cord-008590-xivnsldf 135 32 a a DT cord-008590-xivnsldf 135 33 deacylase deacylase NN cord-008590-xivnsldf 135 34 which which WDT cord-008590-xivnsldf 135 35 removes remove VBZ cord-008590-xivnsldf 135 36 most most JJS cord-008590-xivnsldf 135 37 acetyl acetyl NN cord-008590-xivnsldf 135 38 groups group NNS cord-008590-xivnsldf 135 39 from from IN cord-008590-xivnsldf 135 40 GlcNAc GlcNAc NNP cord-008590-xivnsldf 136 1 ( ( -LRB- cord-008590-xivnsldf 136 2 this this DT cord-008590-xivnsldf 136 3 activity activity NN cord-008590-xivnsldf 136 4 is be VBZ cord-008590-xivnsldf 136 5 low low JJ cord-008590-xivnsldf 136 6 in in IN cord-008590-xivnsldf 136 7 cells cell NNS cord-008590-xivnsldf 136 8 synthesizing synthesize VBG cord-008590-xivnsldf 136 9 heparan heparan NN cord-008590-xivnsldf 136 10 sulfate sulfate NN cord-008590-xivnsldf 136 11 and and CC cord-008590-xivnsldf 136 12 is be VBZ cord-008590-xivnsldf 136 13 stimulated stimulate VBN cord-008590-xivnsldf 136 14 by by IN cord-008590-xivnsldf 136 15 Nsulfation Nsulfation NNP cord-008590-xivnsldf 136 16 at at IN cord-008590-xivnsldf 136 17 other other JJ cord-008590-xivnsldf 136 18 residues residue NNS cord-008590-xivnsldf 136 19 ) ) -RRB- cord-008590-xivnsldf 136 20 , , , cord-008590-xivnsldf 136 21 ( ( -LRB- cord-008590-xivnsldf 136 22 2 2 LS cord-008590-xivnsldf 136 23 ) ) -RRB- cord-008590-xivnsldf 136 24 an an DT cord-008590-xivnsldf 136 25 N N NNP cord-008590-xivnsldf 136 26 - - HYPH cord-008590-xivnsldf 136 27 sulfotransferase sulfotransferase NN cord-008590-xivnsldf 136 28 which which WDT cord-008590-xivnsldf 136 29 acts act VBZ cord-008590-xivnsldf 136 30 on on IN cord-008590-xivnsldf 136 31 most most JJS cord-008590-xivnsldf 136 32 of of IN cord-008590-xivnsldf 136 33 the the DT cord-008590-xivnsldf 136 34 sites site NNS cord-008590-xivnsldf 136 35 exposed expose VBN cord-008590-xivnsldf 136 36 by by IN cord-008590-xivnsldf 136 37 ( ( -LRB- cord-008590-xivnsldf 136 38 I i NN cord-008590-xivnsldf 136 39 ) ) -RRB- cord-008590-xivnsldf 136 40 , , , cord-008590-xivnsldf 136 41 ( ( -LRB- cord-008590-xivnsldf 136 42 3 3 LS cord-008590-xivnsldf 136 43 ) ) -RRB- cord-008590-xivnsldf 136 44 the the DT cord-008590-xivnsldf 136 45 epimerase epimerase NN cord-008590-xivnsldf 136 46 which which WDT cord-008590-xivnsldf 136 47 converts convert VBZ cord-008590-xivnsldf 136 48 70 70 CD cord-008590-xivnsldf 136 49 - - SYM cord-008590-xivnsldf 136 50 90 90 CD cord-008590-xivnsldf 136 51 % % NN cord-008590-xivnsldf 136 52 of of IN cord-008590-xivnsldf 136 53 glucuronic glucuronic JJ cord-008590-xivnsldf 136 54 acid acid NN cord-008590-xivnsldf 136 55 residues residue NNS cord-008590-xivnsldf 136 56 of of IN cord-008590-xivnsldf 136 57 the the DT cord-008590-xivnsldf 136 58 polymer polymer NN cord-008590-xivnsldf 136 59 to to IN cord-008590-xivnsldf 136 60 iduronic iduronic JJ cord-008590-xivnsldf 136 61 acid acid NN cord-008590-xivnsldf 136 62 , , , cord-008590-xivnsldf 136 63 ( ( -LRB- cord-008590-xivnsldf 136 64 4 4 LS cord-008590-xivnsldf 136 65 ) ) -RRB- cord-008590-xivnsldf 136 66 an an DT cord-008590-xivnsldf 136 67 0-sulfotransferase 0-sulfotransferase CD cord-008590-xivnsldf 136 68 which which WDT cord-008590-xivnsldf 136 69 acts act VBZ cord-008590-xivnsldf 136 70 at at IN cord-008590-xivnsldf 136 71 C-2 C-2 NNP cord-008590-xivnsldf 136 72 of of IN cord-008590-xivnsldf 136 73 many many JJ cord-008590-xivnsldf 136 74 iduronic iduronic JJ cord-008590-xivnsldf 136 75 acid acid NN cord-008590-xivnsldf 136 76 residues residue NNS cord-008590-xivnsldf 136 77 , , , cord-008590-xivnsldf 136 78 and and CC cord-008590-xivnsldf 136 79 ( ( -LRB- cord-008590-xivnsldf 136 80 5 5 LS cord-008590-xivnsldf 136 81 ) ) -RRB- cord-008590-xivnsldf 136 82 an an DT cord-008590-xivnsldf 136 83 0-sulfotransferase 0-sulfotransferase CD cord-008590-xivnsldf 136 84 which which WDT cord-008590-xivnsldf 136 85 acts act VBZ cord-008590-xivnsldf 136 86 at at IN cord-008590-xivnsldf 136 87 C-6 C-6 NNP cord-008590-xivnsldf 136 88 of of IN cord-008590-xivnsldf 136 89 glucosamine glucosamine NN cord-008590-xivnsldf 136 90 . . . cord-008590-xivnsldf 137 1 There there EX cord-008590-xivnsldf 137 2 are be VBP cord-008590-xivnsldf 137 3 no no DT cord-008590-xivnsldf 137 4 data datum NNS cord-008590-xivnsldf 137 5 indicating indicate VBG cord-008590-xivnsldf 137 6 whether whether IN cord-008590-xivnsldf 137 7 some some DT cord-008590-xivnsldf 137 8 or or CC cord-008590-xivnsldf 137 9 all all DT cord-008590-xivnsldf 137 10 of of IN cord-008590-xivnsldf 137 11 these these DT cord-008590-xivnsldf 137 12 activities activity NNS cord-008590-xivnsldf 137 13 are be VBP cord-008590-xivnsldf 137 14 expressed express VBN cord-008590-xivnsldf 137 15 in in IN cord-008590-xivnsldf 137 16 the the DT cord-008590-xivnsldf 137 17 same same JJ cord-008590-xivnsldf 137 18 cytologic cytologic JJ cord-008590-xivnsldf 137 19 subcompartment subcompartment NN cord-008590-xivnsldf 137 20 in in IN cord-008590-xivnsldf 137 21 which which WDT cord-008590-xivnsldf 137 22 disaccharide disaccharide JJ cord-008590-xivnsldf 137 23 elongation elongation NN cord-008590-xivnsldf 137 24 occurs occur VBZ cord-008590-xivnsldf 137 25 . . . cord-008590-xivnsldf 138 1 Recent recent JJ cord-008590-xivnsldf 138 2 studies study NNS cord-008590-xivnsldf 138 3 have have VBP cord-008590-xivnsldf 138 4 indicated indicate VBN cord-008590-xivnsldf 138 5 the the DT cord-008590-xivnsldf 138 6 existence existence NN cord-008590-xivnsldf 138 7 of of IN cord-008590-xivnsldf 138 8 two two CD cord-008590-xivnsldf 138 9 classes class NNS cord-008590-xivnsldf 138 10 of of IN cord-008590-xivnsldf 138 11 heparan heparan JJ cord-008590-xivnsldf 138 12 sulfate sulfate NN cord-008590-xivnsldf 138 13 . . . cord-008590-xivnsldf 139 1 One one CD cord-008590-xivnsldf 139 2 is be VBZ cord-008590-xivnsldf 139 3 released release VBN cord-008590-xivnsldf 139 4 from from IN cord-008590-xivnsldf 139 5 the the DT cord-008590-xivnsldf 139 6 cell cell NN cord-008590-xivnsldf 139 7 ( ( -LRB- cord-008590-xivnsldf 139 8 e.g. e.g. RB cord-008590-xivnsldf 139 9 , , , cord-008590-xivnsldf 139 10 hepatocyte hepatocyte NN cord-008590-xivnsldf 139 11 ) ) -RRB- cord-008590-xivnsldf 139 12 ; ; : cord-008590-xivnsldf 139 13 the the DT cord-008590-xivnsldf 139 14 other other JJ cord-008590-xivnsldf 139 15 is be VBZ cord-008590-xivnsldf 139 16 hydrophobic hydrophobic JJ cord-008590-xivnsldf 139 17 and and CC cord-008590-xivnsldf 139 18 membrane membrane NN cord-008590-xivnsldf 139 19 - - HYPH cord-008590-xivnsldf 139 20 associated associate VBN cord-008590-xivnsldf 139 21 . . . cord-008590-xivnsldf 140 1 It -PRON- PRP cord-008590-xivnsldf 140 2 is be VBZ cord-008590-xivnsldf 140 3 thought think VBN cord-008590-xivnsldf 140 4 to to TO cord-008590-xivnsldf 140 5 have have VB cord-008590-xivnsldf 140 6 a a DT cord-008590-xivnsldf 140 7 hydophobic hydophobic JJ cord-008590-xivnsldf 140 8 core core NN cord-008590-xivnsldf 140 9 protein protein NN cord-008590-xivnsldf 140 10 which which WDT cord-008590-xivnsldf 140 11 serves serve VBZ cord-008590-xivnsldf 140 12 as as IN cord-008590-xivnsldf 140 13 anchor anchor NN cord-008590-xivnsldf 140 14 ( ( -LRB- cord-008590-xivnsldf 140 15 66 66 CD cord-008590-xivnsldf 140 16 ) ) -RRB- cord-008590-xivnsldf 140 17 . . . cord-008590-xivnsldf 141 1 Study study NN cord-008590-xivnsldf 141 2 of of IN cord-008590-xivnsldf 141 3 the the DT cord-008590-xivnsldf 141 4 biosynthesis biosynthesis NN cord-008590-xivnsldf 141 5 of of IN cord-008590-xivnsldf 141 6 chondroitin chondroitin NN cord-008590-xivnsldf 141 7 sulfate sulfate NN cord-008590-xivnsldf 141 8 is be VBZ cord-008590-xivnsldf 141 9 potentially potentially RB cord-008590-xivnsldf 141 10 of of IN cord-008590-xivnsldf 141 11 unique unique JJ cord-008590-xivnsldf 141 12 interest interest NN cord-008590-xivnsldf 141 13 for for IN cord-008590-xivnsldf 141 14 defining define VBG cord-008590-xivnsldf 141 15 the the DT cord-008590-xivnsldf 141 16 subcompartmentalization subcompartmentalization NN cord-008590-xivnsldf 141 17 of of IN cord-008590-xivnsldf 141 18 the the DT cord-008590-xivnsldf 141 19 GC GC NNP cord-008590-xivnsldf 141 20 since since IN cord-008590-xivnsldf 141 21 the the DT cord-008590-xivnsldf 141 22 same same JJ cord-008590-xivnsldf 141 23 core core NN cord-008590-xivnsldf 141 24 protein protein NN cord-008590-xivnsldf 141 25 which which WDT cord-008590-xivnsldf 141 26 bears bear VBZ cord-008590-xivnsldf 141 27 80 80 CD cord-008590-xivnsldf 141 28 xylose xylose NN cord-008590-xivnsldf 141 29 - - HYPH cord-008590-xivnsldf 141 30 linked link VBN cord-008590-xivnsldf 141 31 repeating repeat VBG cord-008590-xivnsldf 141 32 disaccharides disaccharide NNS cord-008590-xivnsldf 141 33 also also RB cord-008590-xivnsldf 141 34 bears bear VBZ cord-008590-xivnsldf 141 35 -12 -12 NN cord-008590-xivnsldf 141 36 N n NN cord-008590-xivnsldf 141 37 - - HYPH cord-008590-xivnsldf 141 38 linked link VBN cord-008590-xivnsldf 141 39 complex complex JJ cord-008590-xivnsldf 141 40 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 141 41 , , , cord-008590-xivnsldf 142 1 -120 -120 NNP cord-008590-xivnsldf 142 2 short short JJ cord-008590-xivnsldf 142 3 0-linked 0-linked CD cord-008590-xivnsldf 142 4 GalNAc galnac NN cord-008590-xivnsldf 142 5 - - HYPH cord-008590-xivnsldf 142 6 containing contain VBG cord-008590-xivnsldf 142 7 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 142 8 , , , cord-008590-xivnsldf 142 9 -60 -60 NNP cord-008590-xivnsldf 142 10 single single JJ cord-008590-xivnsldf 142 11 GalNAc galnac NN cord-008590-xivnsldf 142 12 residues residue NNS cord-008590-xivnsldf 142 13 , , , cord-008590-xivnsldf 142 14 and and CC cord-008590-xivnsldf 142 15 ( ( -LRB- cord-008590-xivnsldf 142 16 in in IN cord-008590-xivnsldf 142 17 many many JJ cord-008590-xivnsldf 142 18 cases case NNS cord-008590-xivnsldf 142 19 ) ) -RRB- cord-008590-xivnsldf 143 1 keratan keratan NNP cord-008590-xivnsldf 143 2 sulfate sulfate NNP cord-008590-xivnsldf 143 3 chains chain NNS cord-008590-xivnsldf 143 4 ( ( -LRB- cord-008590-xivnsldf 143 5 44 44 CD cord-008590-xivnsldf 143 6 , , , cord-008590-xivnsldf 143 7 78 78 CD cord-008590-xivnsldf 143 8 , , , cord-008590-xivnsldf 143 9 79 79 CD cord-008590-xivnsldf 143 10 ) ) -RRB- cord-008590-xivnsldf 143 11 . . . cord-008590-xivnsldf 144 1 Such such JJ cord-008590-xivnsldf 144 2 multiple multiple JJ cord-008590-xivnsldf 144 3 derivatization derivatization NN cord-008590-xivnsldf 144 4 of of IN cord-008590-xivnsldf 144 5 a a DT cord-008590-xivnsldf 144 6 single single JJ cord-008590-xivnsldf 144 7 polypeptide polypeptide NN cord-008590-xivnsldf 144 8 provides provide VBZ cord-008590-xivnsldf 144 9 ideal ideal JJ cord-008590-xivnsldf 144 10 material material NN cord-008590-xivnsldf 144 11 for for IN cord-008590-xivnsldf 144 12 establishing establish VBG cord-008590-xivnsldf 144 13 the the DT cord-008590-xivnsldf 144 14 relative relative JJ cord-008590-xivnsldf 144 15 sub sub JJ cord-008590-xivnsldf 144 16 - - JJ cord-008590-xivnsldf 144 17 Golgi golgi JJ cord-008590-xivnsldf 144 18 sites site NNS cord-008590-xivnsldf 144 19 and and CC cord-008590-xivnsldf 144 20 rates rate NNS cord-008590-xivnsldf 144 21 of of IN cord-008590-xivnsldf 144 22 processing processing NN cord-008590-xivnsldf 144 23 of of IN cord-008590-xivnsldf 144 24 such such JJ cord-008590-xivnsldf 144 25 divergent divergent JJ cord-008590-xivnsldf 144 26 structures structure NNS cord-008590-xivnsldf 144 27 . . . cord-008590-xivnsldf 145 1 The the DT cord-008590-xivnsldf 145 2 presently presently RB cord-008590-xivnsldf 145 3 available available JJ cord-008590-xivnsldf 145 4 kinetic kinetic JJ cord-008590-xivnsldf 145 5 data datum NNS cord-008590-xivnsldf 145 6 shown show VBN cord-008590-xivnsldf 145 7 that that IN cord-008590-xivnsldf 145 8 the the DT cord-008590-xivnsldf 145 9 acquisition acquisition NN cord-008590-xivnsldf 145 10 of of IN cord-008590-xivnsldf 145 11 endoglycosaminidase endoglycosaminidase NNP cord-008590-xivnsldf 145 12 H H NNP cord-008590-xivnsldf 145 13 ( ( -LRB- cord-008590-xivnsldf 145 14 endo endo NN cord-008590-xivnsldf 145 15 H h NN cord-008590-xivnsldf 145 16 ) ) -RRB- cord-008590-xivnsldf 145 17 resistance resistance NN cord-008590-xivnsldf 145 18 by by IN cord-008590-xivnsldf 145 19 the the DT cord-008590-xivnsldf 145 20 N n NN cord-008590-xivnsldf 145 21 - - HYPH cord-008590-xivnsldf 145 22 linked link VBN cord-008590-xivnsldf 145 23 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 145 24 of of IN cord-008590-xivnsldf 145 25 chondroitin chondroitin NN cord-008590-xivnsldf 145 26 sulfate sulfate NN cord-008590-xivnsldf 145 27 is be VBZ cord-008590-xivnsldf 145 28 roughly roughly RB cord-008590-xivnsldf 145 29 coincident coincident JJ cord-008590-xivnsldf 145 30 with with IN cord-008590-xivnsldf 145 31 the the DT cord-008590-xivnsldf 145 32 addition addition NN cord-008590-xivnsldf 145 33 of of IN cord-008590-xivnsldf 145 34 the the DT cord-008590-xivnsldf 145 35 repeating repeat VBG cord-008590-xivnsldf 145 36 disaccharides disaccharide NNS cord-008590-xivnsldf 145 37 ( ( -LRB- cord-008590-xivnsldf 145 38 12 12 CD cord-008590-xivnsldf 145 39 ) ) -RRB- cord-008590-xivnsldf 145 40 . . . cord-008590-xivnsldf 146 1 Study study NN cord-008590-xivnsldf 146 2 of of IN cord-008590-xivnsldf 146 3 the the DT cord-008590-xivnsldf 146 4 incorporation incorporation NN cord-008590-xivnsldf 146 5 of of IN cord-008590-xivnsldf 146 6 labeled label VBN cord-008590-xivnsldf 146 7 glucosamine glucosamine NN cord-008590-xivnsldf 146 8 into into IN cord-008590-xivnsldf 146 9 the the DT cord-008590-xivnsldf 146 10 repeating repeat VBG cord-008590-xivnsldf 146 11 disaccharides disaccharide NNS cord-008590-xivnsldf 146 12 and and CC cord-008590-xivnsldf 146 13 the the DT cord-008590-xivnsldf 146 14 internal internal JJ cord-008590-xivnsldf 146 15 residues residue NNS cord-008590-xivnsldf 146 16 of of IN cord-008590-xivnsldf 146 17 the the DT cord-008590-xivnsldf 146 18 short short JJ cord-008590-xivnsldf 146 19 0linked 0linked CD cord-008590-xivnsldf 146 20 units unit NNS cord-008590-xivnsldf 146 21 suggest suggest VBP cord-008590-xivnsldf 146 22 that that IN cord-008590-xivnsldf 146 23 these these DT cord-008590-xivnsldf 146 24 two two CD cord-008590-xivnsldf 146 25 events event NNS cord-008590-xivnsldf 146 26 occur occur VBP cord-008590-xivnsldf 146 27 simultaneously simultaneously RB cord-008590-xivnsldf 146 28 ( ( -LRB- cord-008590-xivnsldf 146 29 141 141 CD cord-008590-xivnsldf 146 30 ) ) -RRB- cord-008590-xivnsldf 146 31 . . . cord-008590-xivnsldf 147 1 Other other JJ cord-008590-xivnsldf 147 2 data datum NNS cord-008590-xivnsldf 147 3 bearing bear VBG cord-008590-xivnsldf 147 4 on on IN cord-008590-xivnsldf 147 5 the the DT cord-008590-xivnsldf 147 6 sub sub JJ cord-008590-xivnsldf 147 7 - - JJ cord-008590-xivnsldf 147 8 Golgi golgi JJ cord-008590-xivnsldf 147 9 localization localization NN cord-008590-xivnsldf 147 10 of of IN cord-008590-xivnsldf 147 11 proteoglycan proteoglycan JJ cord-008590-xivnsldf 147 12 synthesis synthesis NN cord-008590-xivnsldf 147 13 come come VBP cord-008590-xivnsldf 147 14 from from IN cord-008590-xivnsldf 147 15 use use NN cord-008590-xivnsldf 147 16 of of IN cord-008590-xivnsldf 147 17 monensin monensin NNP cord-008590-xivnsldf 147 18 . . . cord-008590-xivnsldf 148 1 Both both DT cord-008590-xivnsldf 148 2 the the DT cord-008590-xivnsldf 148 3 addition addition NN cord-008590-xivnsldf 148 4 of of IN cord-008590-xivnsldf 148 5 repeating repeat VBG cord-008590-xivnsldf 148 6 disaccharides disaccharide NNS cord-008590-xivnsldf 148 7 and and CC cord-008590-xivnsldf 148 8 sulfation sulfation NN cord-008590-xivnsldf 148 9 of of IN cord-008590-xivnsldf 148 10 chondroitin chondroitin NN cord-008590-xivnsldf 148 11 sulfate sulfate NN cord-008590-xivnsldf 148 12 are be VBP cord-008590-xivnsldf 148 13 markedly markedly RB cord-008590-xivnsldf 148 14 reduced reduce VBN cord-008590-xivnsldf 148 15 in in IN cord-008590-xivnsldf 148 16 the the DT cord-008590-xivnsldf 148 17 presence presence NN cord-008590-xivnsldf 148 18 of of IN cord-008590-xivnsldf 148 19 monensin monensin NNP cord-008590-xivnsldf 148 20 though though IN cord-008590-xivnsldf 148 21 the the DT cord-008590-xivnsldf 148 22 pools pool NNS cord-008590-xivnsldf 148 23 of of IN cord-008590-xivnsldf 148 24 ATP ATP NNP cord-008590-xivnsldf 148 25 and and CC cord-008590-xivnsldf 148 26 phosphoadenosine phosphoadenosine NNP cord-008590-xivnsldf 148 27 phosphosulfate phosphosulfate NNP cord-008590-xivnsldf 148 28 are be VBP cord-008590-xivnsldf 148 29 unchanged unchanged JJ cord-008590-xivnsldf 148 30 ( ( -LRB- cord-008590-xivnsldf 148 31 12 12 CD cord-008590-xivnsldf 148 32 , , , cord-008590-xivnsldf 148 33 57 57 CD cord-008590-xivnsldf 148 34 , , , cord-008590-xivnsldf 148 35 90 90 CD cord-008590-xivnsldf 148 36 , , , cord-008590-xivnsldf 148 37 91 91 CD cord-008590-xivnsldf 148 38 ) ) -RRB- cord-008590-xivnsldf 148 39 . . . cord-008590-xivnsldf 149 1 One one PRP cord-008590-xivnsldf 149 2 can can MD cord-008590-xivnsldf 149 3 provisionally provisionally RB cord-008590-xivnsldf 149 4 conclude conclude VB cord-008590-xivnsldf 149 5 that that IN cord-008590-xivnsldf 149 6 these these DT cord-008590-xivnsldf 149 7 events event NNS cord-008590-xivnsldf 149 8 take take VBP cord-008590-xivnsldf 149 9 place place NN cord-008590-xivnsldf 149 10 relatively relatively RB cord-008590-xivnsldf 149 11 late late RB cord-008590-xivnsldf 149 12 during during IN cord-008590-xivnsldf 149 13 transit transit NN cord-008590-xivnsldf 149 14 through through IN cord-008590-xivnsldf 149 15 the the DT cord-008590-xivnsldf 149 16 GC GC NNP cord-008590-xivnsldf 149 17 . . . cord-008590-xivnsldf 150 1 The the DT cord-008590-xivnsldf 150 2 limited limited JJ cord-008590-xivnsldf 150 3 amount amount NN cord-008590-xivnsldf 150 4 of of IN cord-008590-xivnsldf 150 5 chondroitin chondroitin NN cord-008590-xivnsldf 150 6 sulfate sulfate NN cord-008590-xivnsldf 150 7 which which WDT cord-008590-xivnsldf 150 8 is be VBZ cord-008590-xivnsldf 150 9 secreted secrete VBN cord-008590-xivnsldf 150 10 in in IN cord-008590-xivnsldf 150 11 the the DT cord-008590-xivnsldf 150 12 presence presence NN cord-008590-xivnsldf 150 13 of of IN cord-008590-xivnsldf 150 14 monensin monensin NNP cord-008590-xivnsldf 150 15 is be VBZ cord-008590-xivnsldf 150 16 undersulfated undersulfate VBN cord-008590-xivnsldf 150 17 and and CC cord-008590-xivnsldf 150 18 may may MD cord-008590-xivnsldf 150 19 serve serve VB cord-008590-xivnsldf 150 20 as as IN cord-008590-xivnsldf 150 21 a a DT cord-008590-xivnsldf 150 22 model model NN cord-008590-xivnsldf 150 23 for for IN cord-008590-xivnsldf 150 24 certain certain JJ cord-008590-xivnsldf 150 25 achondroplasias achondroplasia NNS cord-008590-xivnsldf 150 26 . . . cord-008590-xivnsldf 151 1 c. c. NNP cord-008590-xivnsldf 151 2 TAILORING TAILORING NNP cord-008590-xivnsldf 151 3 OF of IN cord-008590-xivnsldf 151 4 ASPARAGINE asparagine NN cord-008590-xivnsldf 151 5 - - HYPH cord-008590-xivnsldf 151 6 LINKED link VBN cord-008590-xivnsldf 151 7 OLIGOSACCHARIDES OLIGOSACCHARIDES NNP cord-008590-xivnsldf 151 8 Glycoproteins Glycoproteins NNP cord-008590-xivnsldf 151 9 ( ( -LRB- cord-008590-xivnsldf 151 10 7 7 CD cord-008590-xivnsldf 151 11 , , , cord-008590-xivnsldf 151 12 71 71 CD cord-008590-xivnsldf 151 13 , , , cord-008590-xivnsldf 151 14 113 113 CD cord-008590-xivnsldf 151 15 - - SYM cord-008590-xivnsldf 151 16 115 115 CD cord-008590-xivnsldf 151 17 , , , cord-008590-xivnsldf 151 18 125 125 CD cord-008590-xivnsldf 151 19 , , , cord-008590-xivnsldf 151 20 127 127 CD cord-008590-xivnsldf 151 21 ) ) -RRB- cord-008590-xivnsldf 152 1 With with IN cord-008590-xivnsldf 152 2 the the DT cord-008590-xivnsldf 152 3 realization realization NN cord-008590-xivnsldf 152 4 that that WDT cord-008590-xivnsldf 152 5 preassembled preassemble VBD cord-008590-xivnsldf 152 6 dolichol dolichol NNP cord-008590-xivnsldf 152 7 - - HYPH cord-008590-xivnsldf 152 8 linked link VBN cord-008590-xivnsldf 152 9 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 152 10 are be VBP cord-008590-xivnsldf 152 11 cotranslationally cotranslationally RB cord-008590-xivnsldf 152 12 added add VBN cord-008590-xivnsldf 152 13 to to IN cord-008590-xivnsldf 152 14 selected select VBN cord-008590-xivnsldf 152 15 asparagine asparagine NN cord-008590-xivnsldf 152 16 residues residue NNS cord-008590-xivnsldf 152 17 , , , cord-008590-xivnsldf 152 18 the the DT cord-008590-xivnsldf 152 19 further further JJ cord-008590-xivnsldf 152 20 processing processing NN cord-008590-xivnsldf 152 21 of of IN cord-008590-xivnsldf 152 22 The the DT cord-008590-xivnsldf 152 23 common common JJ cord-008590-xivnsldf 152 24 precursor precursor NN cord-008590-xivnsldf 152 25 at at IN cord-008590-xivnsldf 152 26 the the DT cord-008590-xivnsldf 152 27 top top JJ cord-008590-xivnsldf 152 28 left left NN cord-008590-xivnsldf 152 29 can can MD cord-008590-xivnsldf 152 30 be be VB cord-008590-xivnsldf 152 31 trimmed trim VBN cord-008590-xivnsldf 152 32 and and CC cord-008590-xivnsldf 152 33 further further RB cord-008590-xivnsldf 152 34 processed process VBN cord-008590-xivnsldf 152 35 toward toward IN cord-008590-xivnsldf 152 36 complex complex JJ cord-008590-xivnsldf 152 37 or or CC cord-008590-xivnsldf 152 38 hybrid hybrid JJ cord-008590-xivnsldf 152 39 structures structure NNS cord-008590-xivnsldf 152 40 , , , cord-008590-xivnsldf 152 41 structures structure NNS cord-008590-xivnsldf 152 42 characteristic characteristic JJ cord-008590-xivnsldf 152 43 of of IN cord-008590-xivnsldf 152 44 lysosomal lysosomal JJ cord-008590-xivnsldf 152 45 enzymes enzyme NNS cord-008590-xivnsldf 152 46 ( ( -LRB- cord-008590-xivnsldf 152 47 " " `` cord-008590-xivnsldf 152 48 unblocked unblocked JJ cord-008590-xivnsldf 152 49 unit unit NN cord-008590-xivnsldf 152 50 " " '' cord-008590-xivnsldf 152 51 ) ) -RRB- cord-008590-xivnsldf 152 52 , , , cord-008590-xivnsldf 152 53 or or CC cord-008590-xivnsldf 152 54 keratan keratan NNP cord-008590-xivnsldf 152 55 sulfate sulfate NNP cord-008590-xivnsldf 152 56 . . . cord-008590-xivnsldf 153 1 As as IN cord-008590-xivnsldf 153 2 is be VBZ cord-008590-xivnsldf 153 3 extensively extensively RB cord-008590-xivnsldf 153 4 discussed discuss VBN cord-008590-xivnsldf 153 5 elsewhere elsewhere RB cord-008590-xivnsldf 153 6 ( ( -LRB- cord-008590-xivnsldf 153 7 71,113 71,113 CD cord-008590-xivnsldf 153 8 ) ) -RRB- cord-008590-xivnsldf 153 9 along along IN cord-008590-xivnsldf 153 10 the the DT cord-008590-xivnsldf 153 11 " " `` cord-008590-xivnsldf 153 12 complex complex JJ cord-008590-xivnsldf 153 13 pathway pathway NN cord-008590-xivnsldf 153 14 " " '' cord-008590-xivnsldf 153 15 several several JJ cord-008590-xivnsldf 153 16 options option NNS cord-008590-xivnsldf 153 17 and and CC cord-008590-xivnsldf 153 18 control control NN cord-008590-xivnsldf 153 19 points point NNS cord-008590-xivnsldf 153 20 exist exist VBP cord-008590-xivnsldf 153 21 . . . cord-008590-xivnsldf 154 1 The the DT cord-008590-xivnsldf 154 2 activities activity NNS cord-008590-xivnsldf 154 3 of of IN cord-008590-xivnsldf 154 4 GlcNAc GlcNAc NNP cord-008590-xivnsldf 154 5 transferases transferase NNS cord-008590-xivnsldf 154 6 ( ( -LRB- cord-008590-xivnsldf 154 7 TI TI NNP cord-008590-xivnsldf 154 8 - - HYPH cord-008590-xivnsldf 154 9 TIV TIV NNP cord-008590-xivnsldf 154 10 ) ) -RRB- cord-008590-xivnsldf 154 11 are be VBP cord-008590-xivnsldf 154 12 of of IN cord-008590-xivnsldf 154 13 key key JJ cord-008590-xivnsldf 154 14 importance importance NN cord-008590-xivnsldf 154 15 . . . cord-008590-xivnsldf 155 1 TIII TIII NNP cord-008590-xivnsldf 155 2 , , , cord-008590-xivnsldf 155 3 for for IN cord-008590-xivnsldf 155 4 example example NN cord-008590-xivnsldf 155 5 . . . cord-008590-xivnsldf 156 1 generates generate VBZ cord-008590-xivnsldf 156 2 a a DT cord-008590-xivnsldf 156 3 " " `` cord-008590-xivnsldf 156 4 bisected bisect VBN cord-008590-xivnsldf 156 5 " " '' cord-008590-xivnsldf 156 6 ( ( -LRB- cord-008590-xivnsldf 156 7 bis bis NNP cord-008590-xivnsldf 156 8 ) ) -RRB- cord-008590-xivnsldf 156 9 structure structure NN cord-008590-xivnsldf 156 10 by by IN cord-008590-xivnsldf 156 11 addition addition NN cord-008590-xivnsldf 156 12 of of IN cord-008590-xivnsldf 156 13 GlcNAc GlcNAc NNP cord-008590-xivnsldf 156 14 to to IN cord-008590-xivnsldf 156 15 the the DT cord-008590-xivnsldf 156 16 most most RBS cord-008590-xivnsldf 156 17 internal internal JJ cord-008590-xivnsldf 156 18 ( ( -LRB- cord-008590-xivnsldf 156 19 plinked plinke VBN cord-008590-xivnsldf 156 20 ) ) -RRB- cord-008590-xivnsldf 156 21 mannose mannose NN cord-008590-xivnsldf 156 22 and and CC cord-008590-xivnsldf 156 23 as as IN cord-008590-xivnsldf 156 24 a a DT cord-008590-xivnsldf 156 25 result result NN cord-008590-xivnsldf 156 26 blocks block VBZ cord-008590-xivnsldf 156 27 the the DT cord-008590-xivnsldf 156 28 activity activity NN cord-008590-xivnsldf 156 29 of of IN cord-008590-xivnsldf 156 30 TII TII NNP cord-008590-xivnsldf 156 31 , , , cord-008590-xivnsldf 156 32 TIV TIV NNP cord-008590-xivnsldf 156 33 . . . cord-008590-xivnsldf 157 1 fucosyl fucosyl NNP cord-008590-xivnsldf 157 2 transferase transferase NNP cord-008590-xivnsldf 157 3 , , , cord-008590-xivnsldf 157 4 and and CC cord-008590-xivnsldf 157 5 mannosidase mannosidase NN cord-008590-xivnsldf 157 6 11 11 CD cord-008590-xivnsldf 157 7 . . . cord-008590-xivnsldf 158 1 TI TI NNP cord-008590-xivnsldf 158 2 action action NN cord-008590-xivnsldf 158 3 is be VBZ cord-008590-xivnsldf 158 4 a a DT cord-008590-xivnsldf 158 5 prerequisite prerequisite NN cord-008590-xivnsldf 158 6 for for IN cord-008590-xivnsldf 158 7 action action NN cord-008590-xivnsldf 158 8 of of IN cord-008590-xivnsldf 158 9 several several JJ cord-008590-xivnsldf 158 10 further further JJ cord-008590-xivnsldf 158 11 transferases transferase NNS cord-008590-xivnsldf 158 12 . . . cord-008590-xivnsldf 159 1 The the DT cord-008590-xivnsldf 159 2 structure structure NN cord-008590-xivnsldf 159 3 bearing bear VBG cord-008590-xivnsldf 159 4 GlcNAc glcnac NN cord-008590-xivnsldf 159 5 - - HYPH cord-008590-xivnsldf 159 6 phosphate phosphate NN cord-008590-xivnsldf 159 7 , , , cord-008590-xivnsldf 159 8 which which WDT cord-008590-xivnsldf 159 9 can can MD cord-008590-xivnsldf 159 10 give give VB cord-008590-xivnsldf 159 11 rise rise NN cord-008590-xivnsldf 159 12 to to IN cord-008590-xivnsldf 159 13 an an DT cord-008590-xivnsldf 159 14 unblocked unblocked JJ cord-008590-xivnsldf 159 15 unit unit NN cord-008590-xivnsldf 159 16 , , , cord-008590-xivnsldf 159 17 indicates indicate VBZ cord-008590-xivnsldf 159 18 only only RB cord-008590-xivnsldf 159 19 one one CD cord-008590-xivnsldf 159 20 of of IN cord-008590-xivnsldf 159 21 several several JJ cord-008590-xivnsldf 159 22 possible possible JJ cord-008590-xivnsldf 159 23 phosphorylated phosphorylated JJ cord-008590-xivnsldf 159 24 mannose mannose NN cord-008590-xivnsldf 159 25 residues residue NNS cord-008590-xivnsldf 159 26 . . . cord-008590-xivnsldf 160 1 The the DT cord-008590-xivnsldf 160 2 actual actual JJ cord-008590-xivnsldf 160 3 degree degree NN cord-008590-xivnsldf 160 4 of of IN cord-008590-xivnsldf 160 5 concomitant concomitant JJ cord-008590-xivnsldf 160 6 mannose mannose NN cord-008590-xivnsldf 160 7 trimming trimming NN cord-008590-xivnsldf 160 8 is be VBZ cord-008590-xivnsldf 160 9 progressive progressive JJ cord-008590-xivnsldf 160 10 but but CC cord-008590-xivnsldf 160 11 not not RB cord-008590-xivnsldf 160 12 uniform uniform JJ cord-008590-xivnsldf 160 13 . . . cord-008590-xivnsldf 161 1 Tr Tr NNP cord-008590-xivnsldf 161 2 , , , cord-008590-xivnsldf 161 3 Terminal Terminal NNP cord-008590-xivnsldf 161 4 sugar sugar NN cord-008590-xivnsldf 161 5 ( ( -LRB- cord-008590-xivnsldf 161 6 fucose fucose NNP cord-008590-xivnsldf 161 7 , , , cord-008590-xivnsldf 161 8 Gal Gal NNP cord-008590-xivnsldf 161 9 , , , cord-008590-xivnsldf 161 10 sialyl sialyl NNP cord-008590-xivnsldf 161 11 ) ) -RRB- cord-008590-xivnsldf 161 12 transferases transferase NNS cord-008590-xivnsldf 161 13 ; ; : cord-008590-xivnsldf 161 14 G g NN cord-008590-xivnsldf 161 15 , , , cord-008590-xivnsldf 161 16 glucosidase glucosidase NN cord-008590-xivnsldf 161 17 ; ; : cord-008590-xivnsldf 161 18 M M NNP cord-008590-xivnsldf 161 19 , , , cord-008590-xivnsldf 161 20 mannosidase mannosidase NN cord-008590-xivnsldf 161 21 . . . cord-008590-xivnsldf 162 1 such such JJ cord-008590-xivnsldf 162 2 moieties moiety NNS cord-008590-xivnsldf 162 3 has have VBZ cord-008590-xivnsldf 162 4 been be VBN cord-008590-xivnsldf 162 5 rapidly rapidly RB cord-008590-xivnsldf 162 6 elucidated elucidate VBN cord-008590-xivnsldf 162 7 . . . cord-008590-xivnsldf 163 1 The the DT cord-008590-xivnsldf 163 2 recent recent JJ cord-008590-xivnsldf 163 3 reviews review NNS cord-008590-xivnsldf 163 4 of of IN cord-008590-xivnsldf 163 5 these these DT cord-008590-xivnsldf 163 6 investigations investigation NNS cord-008590-xivnsldf 163 7 are be VBP cord-008590-xivnsldf 163 8 too too RB cord-008590-xivnsldf 163 9 thorough thorough JJ cord-008590-xivnsldf 163 10 to to TO cord-008590-xivnsldf 163 11 warrant warrant VB cord-008590-xivnsldf 163 12 an an DT cord-008590-xivnsldf 163 13 exposition exposition NN cord-008590-xivnsldf 163 14 of of IN cord-008590-xivnsldf 163 15 this this DT cord-008590-xivnsldf 163 16 now now RB cord-008590-xivnsldf 163 17 well well RB cord-008590-xivnsldf 163 18 - - HYPH cord-008590-xivnsldf 163 19 known know VBN cord-008590-xivnsldf 163 20 sequence sequence NN cord-008590-xivnsldf 163 21 of of IN cord-008590-xivnsldf 163 22 steps step NNS cord-008590-xivnsldf 163 23 which which WDT cord-008590-xivnsldf 163 24 involves involve VBZ cord-008590-xivnsldf 163 25 both both DT cord-008590-xivnsldf 163 26 sugar sugar NN cord-008590-xivnsldf 163 27 trimming trimming NN cord-008590-xivnsldf 163 28 and and CC cord-008590-xivnsldf 163 29 terminal terminal NN cord-008590-xivnsldf 163 30 sugar sugar NN cord-008590-xivnsldf 163 31 addition addition NN cord-008590-xivnsldf 163 32 . . . cord-008590-xivnsldf 164 1 The the DT cord-008590-xivnsldf 164 2 processing processing NN cord-008590-xivnsldf 164 3 to to IN cord-008590-xivnsldf 164 4 a a DT cord-008590-xivnsldf 164 5 triantennary triantennary JJ cord-008590-xivnsldf 164 6 complex complex JJ cord-008590-xivnsldf 164 7 unit unit NN cord-008590-xivnsldf 164 8 , , , cord-008590-xivnsldf 164 9 e.g. e.g. RB cord-008590-xivnsldf 164 10 , , , cord-008590-xivnsldf 164 11 of of IN cord-008590-xivnsldf 164 12 the the DT cord-008590-xivnsldf 164 13 G g NN cord-008590-xivnsldf 164 14 protein protein NN cord-008590-xivnsldf 164 15 of of IN cord-008590-xivnsldf 164 16 vesicular vesicular JJ cord-008590-xivnsldf 164 17 stomatitis stomatitis NN cord-008590-xivnsldf 164 18 viruses virus NNS cord-008590-xivnsldf 164 19 ( ( -LRB- cord-008590-xivnsldf 164 20 VSV VSV NNP cord-008590-xivnsldf 164 21 ) ) -RRB- cord-008590-xivnsldf 164 22 in in IN cord-008590-xivnsldf 164 23 infected infect VBN cord-008590-xivnsldf 164 24 CHO CHO NNP cord-008590-xivnsldf 164 25 cells cell NNS cord-008590-xivnsldf 164 26 , , , cord-008590-xivnsldf 164 27 involves involve VBZ cord-008590-xivnsldf 164 28 nine nine CD cord-008590-xivnsldf 164 29 enzymes enzyme NNS cord-008590-xivnsldf 164 30 and and CC cord-008590-xivnsldf 164 31 of of IN cord-008590-xivnsldf 164 32 these these DT cord-008590-xivnsldf 164 33 only only RB cord-008590-xivnsldf 164 34 the the DT cord-008590-xivnsldf 164 35 first first JJ cord-008590-xivnsldf 164 36 two two CD cord-008590-xivnsldf 164 37 glucosidases glucosidase NNS cord-008590-xivnsldf 164 38 and and CC cord-008590-xivnsldf 164 39 possibly possibly RB cord-008590-xivnsldf 164 40 the the DT cord-008590-xivnsldf 164 41 first first JJ cord-008590-xivnsldf 164 42 mannosidase mannosidase NNP cord-008590-xivnsldf 164 43 act act NN cord-008590-xivnsldf 164 44 at at IN cord-008590-xivnsldf 164 45 the the DT cord-008590-xivnsldf 164 46 level level NN cord-008590-xivnsldf 164 47 of of IN cord-008590-xivnsldf 164 48 the the DT cord-008590-xivnsldf 164 49 RER RER NNP cord-008590-xivnsldf 164 50 ( ( -LRB- cord-008590-xivnsldf 164 51 71 71 CD cord-008590-xivnsldf 164 52 , , , cord-008590-xivnsldf 164 53 127 127 CD cord-008590-xivnsldf 164 54 , , , cord-008590-xivnsldf 164 55 135 135 CD cord-008590-xivnsldf 164 56 ) ) -RRB- cord-008590-xivnsldf 164 57 . . . cord-008590-xivnsldf 165 1 The the DT cord-008590-xivnsldf 165 2 rest rest NN cord-008590-xivnsldf 165 3 have have VBP cord-008590-xivnsldf 165 4 been be VBN cord-008590-xivnsldf 165 5 localized localize VBN cord-008590-xivnsldf 165 6 to to IN cord-008590-xivnsldf 165 7 preparative preparative JJ cord-008590-xivnsldf 165 8 Golgi Golgi NNP cord-008590-xivnsldf 165 9 fractions fraction NNS cord-008590-xivnsldf 165 10 . . . cord-008590-xivnsldf 166 1 The the DT cord-008590-xivnsldf 166 2 p p NN cord-008590-xivnsldf 166 3 ( ( -LRB- cord-008590-xivnsldf 166 4 1 1 CD cord-008590-xivnsldf 166 5 - - SYM cord-008590-xivnsldf 166 6 4 4 CD cord-008590-xivnsldf 166 7 ) ) -RRB- cord-008590-xivnsldf 166 8 galactosyltransferase galactosyltransferase NNP cord-008590-xivnsldf 166 9 responsible responsible JJ cord-008590-xivnsldf 166 10 for for IN cord-008590-xivnsldf 166 11 subterminal subterminal JJ cord-008590-xivnsldf 166 12 Gal Gal NNP cord-008590-xivnsldf 166 13 addition addition NN cord-008590-xivnsldf 166 14 is be VBZ cord-008590-xivnsldf 166 15 , , , cord-008590-xivnsldf 166 16 at at IN cord-008590-xivnsldf 166 17 present present NN cord-008590-xivnsldf 166 18 , , , cord-008590-xivnsldf 166 19 the the DT cord-008590-xivnsldf 166 20 best well RBS cord-008590-xivnsldf 166 21 characterized characterize VBN cord-008590-xivnsldf 166 22 Golgi Golgi NNP cord-008590-xivnsldf 166 23 enzyme enzyme NN cord-008590-xivnsldf 166 24 . . . cord-008590-xivnsldf 167 1 Not not RB cord-008590-xivnsldf 167 2 only only RB cord-008590-xivnsldf 167 3 have have VBP cord-008590-xivnsldf 167 4 its -PRON- PRP$ cord-008590-xivnsldf 167 5 enzymological enzymological JJ cord-008590-xivnsldf 167 6 properties property NNS cord-008590-xivnsldf 167 7 been be VBN cord-008590-xivnsldf 167 8 investigated investigate VBN cord-008590-xivnsldf 167 9 in in IN cord-008590-xivnsldf 167 10 several several JJ cord-008590-xivnsldf 167 11 tissues tissue NNS cord-008590-xivnsldf 167 12 , , , cord-008590-xivnsldf 167 13 but but CC cord-008590-xivnsldf 167 14 in in IN cord-008590-xivnsldf 167 15 addition addition NN cord-008590-xivnsldf 167 16 first first JJ cord-008590-xivnsldf 167 17 studies study NNS cord-008590-xivnsldf 167 18 of of IN cord-008590-xivnsldf 167 19 its -PRON- PRP$ cord-008590-xivnsldf 167 20 trans trans JJ cord-008590-xivnsldf 167 21 - - JJ cord-008590-xivnsldf 167 22 membrane membrane JJ cord-008590-xivnsldf 167 23 orientation orientation NN cord-008590-xivnsldf 167 24 ( ( -LRB- cord-008590-xivnsldf 167 25 30 30 CD cord-008590-xivnsldf 167 26 ) ) -RRB- cord-008590-xivnsldf 167 27 and and CC cord-008590-xivnsldf 167 28 biosynthesis biosynthesis NN cord-008590-xivnsldf 167 29 ( ( -LRB- cord-008590-xivnsldf 167 30 132 132 CD cord-008590-xivnsldf 167 31 ) ) -RRB- cord-008590-xivnsldf 167 32 have have VBP cord-008590-xivnsldf 167 33 been be VBN cord-008590-xivnsldf 167 34 reported report VBN cord-008590-xivnsldf 167 35 . . . cord-008590-xivnsldf 168 1 In in IN cord-008590-xivnsldf 168 2 HeLa HeLa NNP cord-008590-xivnsldf 168 3 cells cell VBZ cord-008590-xivnsldf 168 4 the the DT cord-008590-xivnsldf 168 5 transferase transferase NN cord-008590-xivnsldf 168 6 bears bear VBZ cord-008590-xivnsldf 168 7 both both DT cord-008590-xivnsldf 168 8 0-linked 0-linked CD cord-008590-xivnsldf 168 9 and and CC cord-008590-xivnsldf 168 10 endo endo NNP cord-008590-xivnsldf 168 11 H H NNP cord-008590-xivnsldf 168 12 - - HYPH cord-008590-xivnsldf 168 13 resistant resistant JJ cord-008590-xivnsldf 168 14 N n CD cord-008590-xivnsldf 168 15 - - HYPH cord-008590-xivnsldf 168 16 linked link VBN cord-008590-xivnsldf 168 17 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 168 18 ( ( -LRB- cord-008590-xivnsldf 168 19 G. G. NNP cord-008590-xivnsldf 168 20 Strous Strous NNP cord-008590-xivnsldf 168 21 , , , cord-008590-xivnsldf 168 22 personal personal JJ cord-008590-xivnsldf 168 23 communication communication NN cord-008590-xivnsldf 168 24 ) ) -RRB- cord-008590-xivnsldf 168 25 . . . cord-008590-xivnsldf 169 1 Several several JJ cord-008590-xivnsldf 169 2 laboratories laboratory NNS cord-008590-xivnsldf 169 3 have have VBP cord-008590-xivnsldf 169 4 monitored monitor VBN cord-008590-xivnsldf 169 5 attempts attempt NNS cord-008590-xivnsldf 169 6 at at IN cord-008590-xivnsldf 169 7 sucrose sucrose NN cord-008590-xivnsldf 169 8 gradient gradient NN cord-008590-xivnsldf 169 9 Golgi Golgi NNP cord-008590-xivnsldf 169 10 subfractionation subfractionation NN cord-008590-xivnsldf 169 11 with with IN cord-008590-xivnsldf 169 12 the the DT cord-008590-xivnsldf 169 13 use use NN cord-008590-xivnsldf 169 14 of of IN cord-008590-xivnsldf 169 15 this this DT cord-008590-xivnsldf 169 16 set set NN cord-008590-xivnsldf 169 17 of of IN cord-008590-xivnsldf 169 18 enzyme enzyme NN cord-008590-xivnsldf 169 19 activities activity NNS cord-008590-xivnsldf 169 20 . . . cord-008590-xivnsldf 170 1 The the DT cord-008590-xivnsldf 170 2 observations observation NNS cord-008590-xivnsldf 170 3 to to IN cord-008590-xivnsldf 170 4 date date NN cord-008590-xivnsldf 170 5 are be VBP cord-008590-xivnsldf 170 6 fragmentary fragmentary JJ cord-008590-xivnsldf 170 7 and and CC cord-008590-xivnsldf 170 8 the the DT cord-008590-xivnsldf 170 9 separations separation NNS cord-008590-xivnsldf 170 10 only only RB cord-008590-xivnsldf 170 11 partial partial JJ cord-008590-xivnsldf 170 12 . . . cord-008590-xivnsldf 171 1 The the DT cord-008590-xivnsldf 171 2 first first JJ cord-008590-xivnsldf 171 3 mannosidase mannosidase NN cord-008590-xivnsldf 171 4 has have VBZ cord-008590-xivnsldf 171 5 been be VBN cord-008590-xivnsldf 171 6 reported report VBN cord-008590-xivnsldf 171 7 to to TO cord-008590-xivnsldf 171 8 have have VB cord-008590-xivnsldf 171 9 an an DT cord-008590-xivnsldf 171 10 isopycnic isopycnic JJ cord-008590-xivnsldf 171 11 density density NN cord-008590-xivnsldf 171 12 in in IN cord-008590-xivnsldf 171 13 sucrose sucrose NN cord-008590-xivnsldf 171 14 gradients gradient NNS cord-008590-xivnsldf 171 15 which which WDT cord-008590-xivnsldf 171 16 is be VBZ cord-008590-xivnsldf 171 17 higher high JJR cord-008590-xivnsldf 171 18 than than IN cord-008590-xivnsldf 171 19 that that DT cord-008590-xivnsldf 171 20 of of IN cord-008590-xivnsldf 171 21 galactosyltransferase galactosyltransferase NNP cord-008590-xivnsldf 172 1 [ [ -LRB- cord-008590-xivnsldf 172 2 CHO CHO NNP cord-008590-xivnsldf 172 3 cells cell NNS cord-008590-xivnsldf 172 4 ( ( -LRB- cord-008590-xivnsldf 172 5 20 20 CD cord-008590-xivnsldf 172 6 ) ) -RRB- cord-008590-xivnsldf 172 7 and and CC cord-008590-xivnsldf 172 8 liver liver NN cord-008590-xivnsldf 172 9 ( ( -LRB- cord-008590-xivnsldf 172 10 loo loo NNP cord-008590-xivnsldf 172 11 ) ) -RRB- cord-008590-xivnsldf 172 12 ] ] -RRB- cord-008590-xivnsldf 172 13 . . . cord-008590-xivnsldf 173 1 Such such JJ cord-008590-xivnsldf 173 2 a a DT cord-008590-xivnsldf 173 3 difference difference NN cord-008590-xivnsldf 173 4 is be VBZ cord-008590-xivnsldf 173 5 not not RB cord-008590-xivnsldf 173 6 observed observe VBN cord-008590-xivnsldf 173 7 in in IN cord-008590-xivnsldf 173 8 BHK BHK NNP cord-008590-xivnsldf 173 9 cells cell NNS cord-008590-xivnsldf 173 10 even even RB cord-008590-xivnsldf 173 11 when when WRB cord-008590-xivnsldf 173 12 they -PRON- PRP cord-008590-xivnsldf 173 13 are be VBP cord-008590-xivnsldf 173 14 infected infect VBN cord-008590-xivnsldf 173 15 with with IN cord-008590-xivnsldf 173 16 Semliki Semliki NNP cord-008590-xivnsldf 173 17 Forest Forest NNP cord-008590-xivnsldf 173 18 virus virus NN cord-008590-xivnsldf 173 19 and and CC cord-008590-xivnsldf 173 20 treated treat VBN cord-008590-xivnsldf 173 21 with with IN cord-008590-xivnsldf 173 22 monensin monensin NNP cord-008590-xivnsldf 173 23 so so IN cord-008590-xivnsldf 173 24 as as IN cord-008590-xivnsldf 173 25 to to TO cord-008590-xivnsldf 173 26 increase increase VB cord-008590-xivnsldf 173 27 the the DT cord-008590-xivnsldf 173 28 density density NN cord-008590-xivnsldf 173 29 of of IN cord-008590-xivnsldf 173 30 medial medial JJ cord-008590-xivnsldf 173 31 Golgi Golgi NNP cord-008590-xivnsldf 173 32 elements element NNS cord-008590-xivnsldf 173 33 ( ( -LRB- cord-008590-xivnsldf 173 34 101 101 CD cord-008590-xivnsldf 173 35 ) ) -RRB- cord-008590-xivnsldf 173 36 . . . cord-008590-xivnsldf 174 1 In in IN cord-008590-xivnsldf 174 2 the the DT cord-008590-xivnsldf 174 3 case case NN cord-008590-xivnsldf 174 4 of of IN cord-008590-xivnsldf 174 5 CHO CHO NNP cord-008590-xivnsldf 174 6 cells cell VBZ cord-008590-xivnsldf 174 7 the the DT cord-008590-xivnsldf 174 8 density density NN cord-008590-xivnsldf 174 9 differences difference NNS cord-008590-xivnsldf 174 10 are be VBP cord-008590-xivnsldf 174 11 small small JJ cord-008590-xivnsldf 174 12 but but CC cord-008590-xivnsldf 174 13 intriguing intriguing JJ cord-008590-xivnsldf 174 14 since since IN cord-008590-xivnsldf 174 15 the the DT cord-008590-xivnsldf 174 16 mannosidase mannosidase NN cord-008590-xivnsldf 174 17 activity activity NN cord-008590-xivnsldf 174 18 is be VBZ cord-008590-xivnsldf 174 19 coisopycnic coisopycnic JJ cord-008590-xivnsldf 174 20 with with IN cord-008590-xivnsldf 174 21 those those DT cord-008590-xivnsldf 174 22 vesicles vesicle NNS cord-008590-xivnsldf 174 23 in in IN cord-008590-xivnsldf 174 24 which which WDT cord-008590-xivnsldf 174 25 the the DT cord-008590-xivnsldf 174 26 G G NNP cord-008590-xivnsldf 174 27 protein protein NN cord-008590-xivnsldf 174 28 acquires acquire VBZ cord-008590-xivnsldf 174 29 covalently covalently RB cord-008590-xivnsldf 174 30 bound bind VBN cord-008590-xivnsldf 174 31 lipid lipid NN cord-008590-xivnsldf 174 32 ( ( -LRB- cord-008590-xivnsldf 174 33 vide vide NN cord-008590-xivnsldf 174 34 infra infra NN cord-008590-xivnsldf 174 35 ) ) -RRB- cord-008590-xivnsldf 174 36 . . . cord-008590-xivnsldf 175 1 Attempts attempt NNS cord-008590-xivnsldf 175 2 to to TO cord-008590-xivnsldf 175 3 separate separate VB cord-008590-xivnsldf 175 4 subfractions subfraction NNS cord-008590-xivnsldf 175 5 differentially differentially RB cord-008590-xivnsldf 175 6 enriched enrich VBN cord-008590-xivnsldf 175 7 in in IN cord-008590-xivnsldf 175 8 the the DT cord-008590-xivnsldf 175 9 terminal terminal JJ cord-008590-xivnsldf 175 10 sugar sugar NN cord-008590-xivnsldf 175 11 transferases transferase NNS cord-008590-xivnsldf 175 12 ( ( -LRB- cord-008590-xivnsldf 175 13 GlcNAc GlcNAc NNP cord-008590-xivnsldf 175 14 , , , cord-008590-xivnsldf 175 15 Gal Gal NNP cord-008590-xivnsldf 175 16 , , , cord-008590-xivnsldf 175 17 Sialyl Sialyl NNP cord-008590-xivnsldf 175 18 ) ) -RRB- cord-008590-xivnsldf 175 19 by by IN cord-008590-xivnsldf 175 20 sucrose sucrose NN cord-008590-xivnsldf 175 21 gradient gradient NN cord-008590-xivnsldf 175 22 analysis analysis NN cord-008590-xivnsldf 175 23 have have VBP cord-008590-xivnsldf 175 24 failed fail VBN cord-008590-xivnsldf 175 25 ( ( -LRB- cord-008590-xivnsldf 175 26 10 10 CD cord-008590-xivnsldf 175 27 , , , cord-008590-xivnsldf 175 28 147 147 CD cord-008590-xivnsldf 175 29 ) ) -RRB- cord-008590-xivnsldf 175 30 or or CC cord-008590-xivnsldf 175 31 provided provide VBN cord-008590-xivnsldf 175 32 only only RB cord-008590-xivnsldf 175 33 slight slight JJ cord-008590-xivnsldf 175 34 differential differential JJ cord-008590-xivnsldf 175 35 enrichment enrichment NN cord-008590-xivnsldf 175 36 ( ( -LRB- cord-008590-xivnsldf 175 37 34a 34a NNS cord-008590-xivnsldf 175 38 ) ) -RRB- cord-008590-xivnsldf 175 39 . . . cord-008590-xivnsldf 176 1 Both both DT cord-008590-xivnsldf 176 2 affinity affinity NN cord-008590-xivnsldf 176 3 methods method NNS cord-008590-xivnsldf 176 4 and and CC cord-008590-xivnsldf 176 5 countercurrent countercurrent NN cord-008590-xivnsldf 176 6 distribution distribution NN cord-008590-xivnsldf 176 7 studies study NNS cord-008590-xivnsldf 176 8 do do VBP cord-008590-xivnsldf 176 9 discriminate discriminate VB cord-008590-xivnsldf 176 10 between between IN cord-008590-xivnsldf 176 11 liver liver NN cord-008590-xivnsldf 176 12 Golgi Golgi NNP cord-008590-xivnsldf 176 13 subfractions subfraction NNS cord-008590-xivnsldf 176 14 differentially differentially RB cord-008590-xivnsldf 176 15 enriched enrich VBN cord-008590-xivnsldf 176 16 in in IN cord-008590-xivnsldf 176 17 galactosyltransferase galactosyltransferase NN cord-008590-xivnsldf 176 18 vs vs IN cord-008590-xivnsldf 176 19 " " `` cord-008590-xivnsldf 176 20 ER ER NNP cord-008590-xivnsldf 176 21 - - HYPH cord-008590-xivnsldf 176 22 like like JJ cord-008590-xivnsldf 176 23 " " `` cord-008590-xivnsldf 176 24 enzyme enzyme NN cord-008590-xivnsldf 176 25 activities activity NNS cord-008590-xivnsldf 176 26 ( ( -LRB- cord-008590-xivnsldf 176 27 49 49 CD cord-008590-xivnsldf 176 28 , , , cord-008590-xivnsldf 176 29 53 53 CD cord-008590-xivnsldf 176 30 ) ) -RRB- cord-008590-xivnsldf 176 31 . . . cord-008590-xivnsldf 177 1 An an DT cord-008590-xivnsldf 177 2 outstanding outstanding JJ cord-008590-xivnsldf 177 3 only only RB cord-008590-xivnsldf 177 4 partly partly RB cord-008590-xivnsldf 177 5 resolved resolve VBD cord-008590-xivnsldf 177 6 question question NN cord-008590-xivnsldf 177 7 is be VBZ cord-008590-xivnsldf 177 8 what what WP cord-008590-xivnsldf 177 9 governs govern VBZ cord-008590-xivnsldf 177 10 the the DT cord-008590-xivnsldf 177 11 particular particular JJ cord-008590-xivnsldf 177 12 path path NN cord-008590-xivnsldf 177 13 and and CC cord-008590-xivnsldf 177 14 degree degree NN cord-008590-xivnsldf 177 15 of of IN cord-008590-xivnsldf 177 16 processing processing NN cord-008590-xivnsldf 177 17 at at IN cord-008590-xivnsldf 177 18 a a DT cord-008590-xivnsldf 177 19 given give VBN cord-008590-xivnsldf 177 20 glycosylation glycosylation NN cord-008590-xivnsldf 177 21 site site NN cord-008590-xivnsldf 177 22 . . . cord-008590-xivnsldf 178 1 Among among IN cord-008590-xivnsldf 178 2 the the DT cord-008590-xivnsldf 178 3 key key JJ cord-008590-xivnsldf 178 4 considerations consideration NNS cord-008590-xivnsldf 178 5 is be VBZ cord-008590-xivnsldf 178 6 the the DT cord-008590-xivnsldf 178 7 repertoire repertoire NN cord-008590-xivnsldf 178 8 of of IN cord-008590-xivnsldf 178 9 processing processing NN cord-008590-xivnsldf 178 10 activities activity NNS cord-008590-xivnsldf 178 11 of of IN cord-008590-xivnsldf 178 12 a a DT cord-008590-xivnsldf 178 13 given give VBN cord-008590-xivnsldf 178 14 tissue tissue NN cord-008590-xivnsldf 178 15 , , , cord-008590-xivnsldf 178 16 possible possible JJ cord-008590-xivnsldf 178 17 competition competition NN cord-008590-xivnsldf 178 18 between between IN cord-008590-xivnsldf 178 19 transferases transferase NNS cord-008590-xivnsldf 178 20 of of IN cord-008590-xivnsldf 178 21 overlapping overlap VBG cord-008590-xivnsldf 178 22 specicificity specicificity NN cord-008590-xivnsldf 178 23 ( ( -LRB- cord-008590-xivnsldf 178 24 7 7 CD cord-008590-xivnsldf 178 25 , , , cord-008590-xivnsldf 178 26 1 1 CD cord-008590-xivnsldf 178 27 13 13 CD cord-008590-xivnsldf 178 28 ) ) -RRB- cord-008590-xivnsldf 178 29 , , , cord-008590-xivnsldf 178 30 and and CC cord-008590-xivnsldf 178 31 the the DT cord-008590-xivnsldf 178 32 steric steric JJ cord-008590-xivnsldf 178 33 environment environment NN cord-008590-xivnsldf 178 34 of of IN cord-008590-xivnsldf 178 35 the the DT cord-008590-xivnsldf 178 36 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 178 37 . . . cord-008590-xivnsldf 179 1 In in IN cord-008590-xivnsldf 179 2 the the DT cord-008590-xivnsldf 179 3 case case NN cord-008590-xivnsldf 179 4 of of IN cord-008590-xivnsldf 179 5 thyroglobulin thyroglobulin NNP cord-008590-xivnsldf 179 6 ( ( -LRB- cord-008590-xivnsldf 179 7 127 127 CD cord-008590-xivnsldf 179 8 ) ) -RRB- cord-008590-xivnsldf 179 9 , , , cord-008590-xivnsldf 179 10 certain certain JJ cord-008590-xivnsldf 179 11 IgM IgM NNP cord-008590-xivnsldf 179 12 myeloma myeloma NN cord-008590-xivnsldf 179 13 proteins protein NNS cord-008590-xivnsldf 179 14 ( ( -LRB- cord-008590-xivnsldf 179 15 I i NN cord-008590-xivnsldf 179 16 ) ) -RRB- cord-008590-xivnsldf 179 17 and and CC cord-008590-xivnsldf 179 18 certain certain JJ cord-008590-xivnsldf 179 19 viral viral JJ cord-008590-xivnsldf 179 20 envelope envelope NN cord-008590-xivnsldf 179 21 glycoproteins glycoprotein VBZ cord-008590-xivnsldf 179 22 the the DT cord-008590-xivnsldf 179 23 situation situation NN cord-008590-xivnsldf 179 24 is be VBZ cord-008590-xivnsldf 179 25 particularly particularly RB cord-008590-xivnsldf 179 26 intriguing intriguing JJ cord-008590-xivnsldf 179 27 since since IN cord-008590-xivnsldf 179 28 different different JJ cord-008590-xivnsldf 179 29 sites site NNS cord-008590-xivnsldf 179 30 along along IN cord-008590-xivnsldf 179 31 a a DT cord-008590-xivnsldf 179 32 single single JJ cord-008590-xivnsldf 179 33 polypeptide polypeptide NN cord-008590-xivnsldf 179 34 ungergo ungergo NN cord-008590-xivnsldf 179 35 radically radically RB cord-008590-xivnsldf 179 36 different different JJ cord-008590-xivnsldf 179 37 degrees degree NNS cord-008590-xivnsldf 179 38 of of IN cord-008590-xivnsldf 179 39 processing processing NN cord-008590-xivnsldf 179 40 . . . cord-008590-xivnsldf 180 1 In in IN cord-008590-xivnsldf 180 2 this this DT cord-008590-xivnsldf 180 3 last last RB cord-008590-xivnsldf 180 4 - - HYPH cord-008590-xivnsldf 180 5 mentioned mention VBN cord-008590-xivnsldf 180 6 case case NN cord-008590-xivnsldf 180 7 , , , cord-008590-xivnsldf 180 8 it -PRON- PRP cord-008590-xivnsldf 180 9 has have VBZ cord-008590-xivnsldf 180 10 been be VBN cord-008590-xivnsldf 180 11 observed observe VBN cord-008590-xivnsldf 180 12 that that IN cord-008590-xivnsldf 180 13 when when WRB cord-008590-xivnsldf 180 14 Sindbis Sindbis NNP cord-008590-xivnsldf 180 15 virus virus NN cord-008590-xivnsldf 180 16 infects infect VBZ cord-008590-xivnsldf 180 17 different different JJ cord-008590-xivnsldf 180 18 host host NN cord-008590-xivnsldf 180 19 cells cell NNS cord-008590-xivnsldf 180 20 different different JJ cord-008590-xivnsldf 180 21 sites site NNS cord-008590-xivnsldf 180 22 are be VBP cord-008590-xivnsldf 180 23 processed process VBN cord-008590-xivnsldf 180 24 to to IN cord-008590-xivnsldf 180 25 different different JJ cord-008590-xivnsldf 180 26 extents extent NNS cord-008590-xivnsldf 180 27 ( ( -LRB- cord-008590-xivnsldf 180 28 125 125 CD cord-008590-xivnsldf 180 29 ) ) -RRB- cord-008590-xivnsldf 180 30 . . . cord-008590-xivnsldf 181 1 Additional additional JJ cord-008590-xivnsldf 181 2 variables variable NNS cord-008590-xivnsldf 181 3 in in IN cord-008590-xivnsldf 181 4 terminal terminal JJ cord-008590-xivnsldf 181 5 sugar sugar NN cord-008590-xivnsldf 181 6 addition addition NN cord-008590-xivnsldf 181 7 concern concern NN cord-008590-xivnsldf 181 8 ( ( -LRB- cord-008590-xivnsldf 181 9 1 1 LS cord-008590-xivnsldf 181 10 ) ) -RRB- cord-008590-xivnsldf 181 11 the the DT cord-008590-xivnsldf 181 12 degree degree NN cord-008590-xivnsldf 181 13 to to TO cord-008590-xivnsldf 181 14 which which WDT cord-008590-xivnsldf 181 15 the the DT cord-008590-xivnsldf 181 16 transferases transferase NNS cord-008590-xivnsldf 181 17 may may MD cord-008590-xivnsldf 181 18 be be VB cord-008590-xivnsldf 181 19 present present JJ cord-008590-xivnsldf 181 20 at at IN cord-008590-xivnsldf 181 21 the the DT cord-008590-xivnsldf 181 22 cell cell NN cord-008590-xivnsldf 181 23 surface surface NN cord-008590-xivnsldf 181 24 as as RB cord-008590-xivnsldf 181 25 well well RB cord-008590-xivnsldf 181 26 as as IN cord-008590-xivnsldf 181 27 in in IN cord-008590-xivnsldf 181 28 the the DT cord-008590-xivnsldf 181 29 GC GC NNP cord-008590-xivnsldf 181 30 , , , cord-008590-xivnsldf 181 31 and and CC cord-008590-xivnsldf 181 32 ( ( -LRB- cord-008590-xivnsldf 181 33 2 2 LS cord-008590-xivnsldf 181 34 ) ) -RRB- cord-008590-xivnsldf 181 35 the the DT cord-008590-xivnsldf 181 36 apparent apparent JJ cord-008590-xivnsldf 181 37 intracellular intracellular JJ cord-008590-xivnsldf 181 38 pool pool NN cord-008590-xivnsldf 181 39 size size NN cord-008590-xivnsldf 181 40 of of IN cord-008590-xivnsldf 181 41 secretory secretory JJ cord-008590-xivnsldf 181 42 glycoproteins glycoprotein NNS cord-008590-xivnsldf 181 43 which which WDT cord-008590-xivnsldf 181 44 have have VBP cord-008590-xivnsldf 181 45 already already RB cord-008590-xivnsldf 181 46 accepted accept VBN cord-008590-xivnsldf 181 47 their -PRON- PRP$ cord-008590-xivnsldf 181 48 terminal terminal JJ cord-008590-xivnsldf 181 49 sugars sugar NNS cord-008590-xivnsldf 181 50 . . . cord-008590-xivnsldf 182 1 Both both DT cord-008590-xivnsldf 182 2 enzymologic enzymologic NN cord-008590-xivnsldf 182 3 ( ( -LRB- cord-008590-xivnsldf 182 4 122 122 CD cord-008590-xivnsldf 182 5 ) ) -RRB- cord-008590-xivnsldf 182 6 and and CC cord-008590-xivnsldf 182 7 , , , cord-008590-xivnsldf 182 8 less less RBR cord-008590-xivnsldf 182 9 equivocally equivocally RB cord-008590-xivnsldf 182 10 , , , cord-008590-xivnsldf 182 11 immunocytochemical immunocytochemical NNP cord-008590-xivnsldf 182 12 studies study NNS cord-008590-xivnsldf 182 13 ( ( -LRB- cord-008590-xivnsldf 182 14 99 99 CD cord-008590-xivnsldf 182 15 ) ) -RRB- cord-008590-xivnsldf 182 16 show show VBP cord-008590-xivnsldf 182 17 that that IN cord-008590-xivnsldf 182 18 in in IN cord-008590-xivnsldf 182 19 selected select VBN cord-008590-xivnsldf 182 20 cells cell NNS cord-008590-xivnsldf 182 21 galactosyltransferase galactosyltransferase NN cord-008590-xivnsldf 182 22 may may MD cord-008590-xivnsldf 182 23 be be VB cord-008590-xivnsldf 182 24 exposed expose VBN cord-008590-xivnsldf 182 25 at at IN cord-008590-xivnsldf 182 26 the the DT cord-008590-xivnsldf 182 27 ( ( -LRB- cord-008590-xivnsldf 182 28 apical apical JJ cord-008590-xivnsldf 182 29 ) ) -RRB- cord-008590-xivnsldf 182 30 cell cell NN cord-008590-xivnsldf 182 31 membrane membrane NN cord-008590-xivnsldf 182 32 . . . cord-008590-xivnsldf 183 1 Presumably presumably RB cord-008590-xivnsldf 183 2 this this DT cord-008590-xivnsldf 183 3 is be VBZ cord-008590-xivnsldf 183 4 a a DT cord-008590-xivnsldf 183 5 reflection reflection NN cord-008590-xivnsldf 183 6 of of IN cord-008590-xivnsldf 183 7 some some DT cord-008590-xivnsldf 183 8 structural structural JJ cord-008590-xivnsldf 183 9 change change NN cord-008590-xivnsldf 183 10 in in IN cord-008590-xivnsldf 183 11 the the DT cord-008590-xivnsldf 183 12 enzyme enzyme NN cord-008590-xivnsldf 183 13 or or CC cord-008590-xivnsldf 183 14 other other JJ cord-008590-xivnsldf 183 15 determinative determinative JJ cord-008590-xivnsldf 183 16 Golgi Golgi NNP cord-008590-xivnsldf 183 17 elements element NNS cord-008590-xivnsldf 183 18 which which WDT cord-008590-xivnsldf 183 19 abrogates abrogate VBZ cord-008590-xivnsldf 183 20 the the DT cord-008590-xivnsldf 183 21 normal normal JJ cord-008590-xivnsldf 183 22 affinity affinity NN cord-008590-xivnsldf 183 23 of of IN cord-008590-xivnsldf 183 24 the the DT cord-008590-xivnsldf 183 25 enzyme enzyme NN cord-008590-xivnsldf 183 26 for for IN cord-008590-xivnsldf 183 27 the the DT cord-008590-xivnsldf 183 28 GC GC NNP cord-008590-xivnsldf 183 29 . . . cord-008590-xivnsldf 184 1 With with IN cord-008590-xivnsldf 184 2 respect respect NN cord-008590-xivnsldf 184 3 to to IN cord-008590-xivnsldf 184 4 ( ( -LRB- cord-008590-xivnsldf 184 5 2 2 LS cord-008590-xivnsldf 184 6 ) ) -RRB- cord-008590-xivnsldf 184 7 , , , cord-008590-xivnsldf 184 8 comparative comparative JJ cord-008590-xivnsldf 184 9 studies study NNS cord-008590-xivnsldf 184 10 of of IN cord-008590-xivnsldf 184 11 immunoglobulin immunoglobulin NN cord-008590-xivnsldf 184 12 synthesis synthesis NN cord-008590-xivnsldf 184 13 by by IN cord-008590-xivnsldf 184 14 several several JJ cord-008590-xivnsldf 184 15 mouse mouse NN cord-008590-xivnsldf 184 16 myelomas myeloma NNS cord-008590-xivnsldf 184 17 indicate indicate VBP cord-008590-xivnsldf 184 18 that that IN cord-008590-xivnsldf 184 19 the the DT cord-008590-xivnsldf 184 20 intracellular intracellular JJ cord-008590-xivnsldf 184 21 pool pool NN cord-008590-xivnsldf 184 22 of of IN cord-008590-xivnsldf 184 23 Ig Ig NNP cord-008590-xivnsldf 184 24 which which WDT cord-008590-xivnsldf 184 25 can can MD cord-008590-xivnsldf 184 26 be be VB cord-008590-xivnsldf 184 27 labeled label VBN cord-008590-xivnsldf 184 28 with with IN cord-008590-xivnsldf 184 29 terminal terminal JJ cord-008590-xivnsldf 184 30 sugars sugar NNS cord-008590-xivnsldf 184 31 may may MD cord-008590-xivnsldf 184 32 be be VB cord-008590-xivnsldf 184 33 either either CC cord-008590-xivnsldf 184 34 undetectably undetectably RB cord-008590-xivnsldf 184 35 small small JJ cord-008590-xivnsldf 184 36 or or CC cord-008590-xivnsldf 184 37 quite quite RB cord-008590-xivnsldf 184 38 substantial substantial JJ cord-008590-xivnsldf 184 39 ( ( -LRB- cord-008590-xivnsldf 184 40 81 81 CD cord-008590-xivnsldf 184 41 , , , cord-008590-xivnsldf 184 42 97 97 CD cord-008590-xivnsldf 184 43 ) ) -RRB- cord-008590-xivnsldf 184 44 . . . cord-008590-xivnsldf 185 1 These these DT cord-008590-xivnsldf 185 2 variations variation NNS cord-008590-xivnsldf 185 3 may may MD cord-008590-xivnsldf 185 4 parallel parallel VB cord-008590-xivnsldf 185 5 the the DT cord-008590-xivnsldf 185 6 degree degree NN cord-008590-xivnsldf 185 7 of of IN cord-008590-xivnsldf 185 8 differentiation differentiation NN cord-008590-xivnsldf 185 9 of of IN cord-008590-xivnsldf 185 10 the the DT cord-008590-xivnsldf 185 11 myeloma myeloma NN cord-008590-xivnsldf 185 12 in in IN cord-008590-xivnsldf 185 13 question question NN cord-008590-xivnsldf 185 14 . . . cord-008590-xivnsldf 186 1 A a DT cord-008590-xivnsldf 186 2 variable variable JJ cord-008590-xivnsldf 186 3 location location NN cord-008590-xivnsldf 186 4 of of IN cord-008590-xivnsldf 186 5 the the DT cord-008590-xivnsldf 186 6 terminal terminal JJ cord-008590-xivnsldf 186 7 sugar sugar NN cord-008590-xivnsldf 186 8 transferases transferase NNS cord-008590-xivnsldf 186 9 might may MD cord-008590-xivnsldf 186 10 explain explain VB cord-008590-xivnsldf 186 11 such such JJ cord-008590-xivnsldf 186 12 observations observation NNS cord-008590-xivnsldf 186 13 , , , cord-008590-xivnsldf 186 14 i.e. i.e. FW cord-008590-xivnsldf 186 15 , , , cord-008590-xivnsldf 186 16 in in IN cord-008590-xivnsldf 186 17 some some DT cord-008590-xivnsldf 186 18 cases case NNS cord-008590-xivnsldf 186 19 they -PRON- PRP cord-008590-xivnsldf 186 20 might may MD cord-008590-xivnsldf 186 21 act act VB cord-008590-xivnsldf 186 22 later later RB cord-008590-xivnsldf 186 23 along along IN cord-008590-xivnsldf 186 24 the the DT cord-008590-xivnsldf 186 25 path path NN cord-008590-xivnsldf 186 26 of of IN cord-008590-xivnsldf 186 27 ICT ICT NNP cord-008590-xivnsldf 186 28 than than IN cord-008590-xivnsldf 186 29 in in IN cord-008590-xivnsldf 186 30 others other NNS cord-008590-xivnsldf 186 31 . . . cord-008590-xivnsldf 187 1 Several several JJ cord-008590-xivnsldf 187 2 cytologic cytologic JJ cord-008590-xivnsldf 187 3 studies study NNS cord-008590-xivnsldf 187 4 pertain pertain VBP cord-008590-xivnsldf 187 5 to to IN cord-008590-xivnsldf 187 6 this this DT cord-008590-xivnsldf 187 7 pathway pathway NN cord-008590-xivnsldf 187 8 . . . cord-008590-xivnsldf 188 1 An an DT cord-008590-xivnsldf 188 2 immunocytochemical immunocytochemical JJ cord-008590-xivnsldf 188 3 study study NN cord-008590-xivnsldf 188 4 has have VBZ cord-008590-xivnsldf 188 5 localized localize VBN cord-008590-xivnsldf 188 6 p p NN cord-008590-xivnsldf 188 7 ( ( -LRB- cord-008590-xivnsldf 188 8 1 1 CD cord-008590-xivnsldf 188 9 - - SYM cord-008590-xivnsldf 188 10 4 4 CD cord-008590-xivnsldf 188 11 ) ) -RRB- cord-008590-xivnsldf 188 12 galactosyltransferase galactosyltransferase NNP cord-008590-xivnsldf 188 13 to to IN cord-008590-xivnsldf 188 14 the the DT cord-008590-xivnsldf 188 15 distal distal JJ cord-008590-xivnsldf 188 16 ( ( -LRB- cord-008590-xivnsldf 188 17 thiamine thiamine NN cord-008590-xivnsldf 188 18 pyrophosphatase pyrophosphatase NN cord-008590-xivnsldf 188 19 - - HYPH cord-008590-xivnsldf 188 20 positive positive JJ cord-008590-xivnsldf 188 21 ) ) -RRB- cord-008590-xivnsldf 188 22 cisternae cisternae NN cord-008590-xivnsldf 188 23 of of IN cord-008590-xivnsldf 188 24 the the DT cord-008590-xivnsldf 188 25 GC GC NNP cord-008590-xivnsldf 188 26 of of IN cord-008590-xivnsldf 188 27 HeLa HeLa NNP cord-008590-xivnsldf 188 28 cells cell NNS cord-008590-xivnsldf 188 29 ( ( -LRB- cord-008590-xivnsldf 188 30 1 1 CD cord-008590-xivnsldf 188 31 10 10 CD cord-008590-xivnsldf 188 32 ) ) -RRB- cord-008590-xivnsldf 188 33 . . . cord-008590-xivnsldf 189 1 The the DT cord-008590-xivnsldf 189 2 presumed presumed JJ cord-008590-xivnsldf 189 3 products product NNS cord-008590-xivnsldf 189 4 of of IN cord-008590-xivnsldf 189 5 terminal terminal JJ cord-008590-xivnsldf 189 6 sugar sugar NN cord-008590-xivnsldf 189 7 transferases transferase NNS cord-008590-xivnsldf 189 8 have have VBP cord-008590-xivnsldf 189 9 also also RB cord-008590-xivnsldf 189 10 been be VBN cord-008590-xivnsldf 189 11 localized localize VBN cord-008590-xivnsldf 189 12 . . . cord-008590-xivnsldf 190 1 Concanavalin Concanavalin NNP cord-008590-xivnsldf 190 2 A A NNP cord-008590-xivnsldf 190 3 - - HYPH cord-008590-xivnsldf 190 4 gold gold NN cord-008590-xivnsldf 190 5 conjugates conjugate NNS cord-008590-xivnsldf 190 6 ( ( -LRB- cord-008590-xivnsldf 190 7 which which WDT cord-008590-xivnsldf 190 8 interact interact VBP cord-008590-xivnsldf 190 9 primarily primarily RB cord-008590-xivnsldf 190 10 with with IN cord-008590-xivnsldf 190 11 immature immature JJ cord-008590-xivnsldf 190 12 asparagine asparagine NN cord-008590-xivnsldf 190 13 - - HYPH cord-008590-xivnsldf 190 14 linked link VBN cord-008590-xivnsldf 190 15 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 190 16 ) ) -RRB- cord-008590-xivnsldf 190 17 stain stain VBP cord-008590-xivnsldf 190 18 the the DT cord-008590-xivnsldf 190 19 cisternal cisternal JJ cord-008590-xivnsldf 190 20 space space NN cord-008590-xivnsldf 190 21 of of IN cord-008590-xivnsldf 190 22 the the DT cord-008590-xivnsldf 190 23 RER rer NN cord-008590-xivnsldf 190 24 and and CC cord-008590-xivnsldf 190 25 all all DT cord-008590-xivnsldf 190 26 Golgi Golgi NNP cord-008590-xivnsldf 190 27 cisternae cisternae NNP cord-008590-xivnsldf 190 28 , , , cord-008590-xivnsldf 190 29 whereas whereas IN cord-008590-xivnsldf 190 30 ricin ricin NN cord-008590-xivnsldf 190 31 120 120 CD cord-008590-xivnsldf 190 32 ( ( -LRB- cord-008590-xivnsldf 190 33 which which WDT cord-008590-xivnsldf 190 34 interacts interact VBZ cord-008590-xivnsldf 190 35 with with IN cord-008590-xivnsldf 190 36 nonreducing nonreducing NNP cord-008590-xivnsldf 190 37 galactosyl galactosyl NNP cord-008590-xivnsldf 190 38 residues residue NNS cord-008590-xivnsldf 190 39 ) ) -RRB- cord-008590-xivnsldf 190 40 stains stain VBZ cord-008590-xivnsldf 190 41 only only RB cord-008590-xivnsldf 190 42 the the DT cord-008590-xivnsldf 190 43 more more RBR cord-008590-xivnsldf 190 44 distal distal JJ cord-008590-xivnsldf 190 45 cisternae cisternae NN cord-008590-xivnsldf 190 46 of of IN cord-008590-xivnsldf 190 47 BHK BHK NNP cord-008590-xivnsldf 190 48 cells cell NNS cord-008590-xivnsldf 190 49 ( ( -LRB- cord-008590-xivnsldf 190 50 36 36 CD cord-008590-xivnsldf 190 51 , , , cord-008590-xivnsldf 190 52 37 37 CD cord-008590-xivnsldf 190 53 ) ) -RRB- cord-008590-xivnsldf 190 54 . . . cord-008590-xivnsldf 191 1 In in IN cord-008590-xivnsldf 191 2 a a DT cord-008590-xivnsldf 191 3 methodologically methodologically RB cord-008590-xivnsldf 191 4 quite quite RB cord-008590-xivnsldf 191 5 different different JJ cord-008590-xivnsldf 191 6 study study NN cord-008590-xivnsldf 191 7 of of IN cord-008590-xivnsldf 191 8 IgM IgM NNP cord-008590-xivnsldf 191 9 - - HYPH cord-008590-xivnsldf 191 10 secreting secrete VBG cord-008590-xivnsldf 191 11 myeloma myeloma NN cord-008590-xivnsldf 191 12 cells cell NNS cord-008590-xivnsldf 191 13 and and CC cord-008590-xivnsldf 191 14 an an DT cord-008590-xivnsldf 191 15 Ig ig JJ cord-008590-xivnsldf 191 16 - - HYPH cord-008590-xivnsldf 191 17 negative negative JJ cord-008590-xivnsldf 191 18 mutant mutant NN cord-008590-xivnsldf 191 19 , , , cord-008590-xivnsldf 191 20 concanavalin concanavalin NNP cord-008590-xivnsldf 191 21 A A NNP cord-008590-xivnsldf 191 22 - - HYPH cord-008590-xivnsldf 191 23 peroxidase peroxidase NN cord-008590-xivnsldf 191 24 has have VBZ cord-008590-xivnsldf 191 25 been be VBN cord-008590-xivnsldf 191 26 shown show VBN cord-008590-xivnsldf 191 27 to to TO cord-008590-xivnsldf 191 28 stain stain VB cord-008590-xivnsldf 191 29 the the DT cord-008590-xivnsldf 191 30 cisternal cisternal JJ cord-008590-xivnsldf 191 31 space space NN cord-008590-xivnsldf 191 32 of of IN cord-008590-xivnsldf 191 33 both both CC cord-008590-xivnsldf 191 34 the the DT cord-008590-xivnsldf 191 35 RER RER NNP cord-008590-xivnsldf 191 36 and and CC cord-008590-xivnsldf 191 37 proximal proximal JJ cord-008590-xivnsldf 191 38 cisternae cisternae NNP cord-008590-xivnsldf 191 39 while while IN cord-008590-xivnsldf 191 40 a a DT cord-008590-xivnsldf 191 41 wheat wheat NN cord-008590-xivnsldf 191 42 germ germ NN cord-008590-xivnsldf 191 43 agglutinin agglutinin NNS cord-008590-xivnsldf 191 44 - - HYPH cord-008590-xivnsldf 191 45 peroxidase peroxidase NN cord-008590-xivnsldf 191 46 conjugate conjugate NN cord-008590-xivnsldf 191 47 ( ( -LRB- cord-008590-xivnsldf 191 48 specific specific JJ cord-008590-xivnsldf 191 49 for for IN cord-008590-xivnsldf 191 50 clustered clustered JJ cord-008590-xivnsldf 191 51 sialic sialic JJ cord-008590-xivnsldf 191 52 acid acid NN cord-008590-xivnsldf 191 53 ) ) -RRB- cord-008590-xivnsldf 191 54 stains stain VBZ cord-008590-xivnsldf 191 55 the the DT cord-008590-xivnsldf 191 56 cisternal cisternal JJ cord-008590-xivnsldf 191 57 space space NN cord-008590-xivnsldf 191 58 of of IN cord-008590-xivnsldf 191 59 more more RBR cord-008590-xivnsldf 191 60 distal distal JJ cord-008590-xivnsldf 191 61 cisternae cisternae NN cord-008590-xivnsldf 191 62 and and CC cord-008590-xivnsldf 191 63 associated associated JJ cord-008590-xivnsldf 191 64 vesicles vesicle NNS cord-008590-xivnsldf 191 65 ( ( -LRB- cord-008590-xivnsldf 191 66 138 138 CD cord-008590-xivnsldf 191 67 ) ) -RRB- cord-008590-xivnsldf 191 68 . . . cord-008590-xivnsldf 192 1 Any any DT cord-008590-xivnsldf 192 2 such such JJ cord-008590-xivnsldf 192 3 studies study NNS cord-008590-xivnsldf 192 4 must must MD cord-008590-xivnsldf 192 5 be be VB cord-008590-xivnsldf 192 6 interpreted interpret VBN cord-008590-xivnsldf 192 7 in in IN cord-008590-xivnsldf 192 8 light light NN cord-008590-xivnsldf 192 9 of of IN cord-008590-xivnsldf 192 10 what what WP cord-008590-xivnsldf 192 11 is be VBZ cord-008590-xivnsldf 192 12 known know VBN cord-008590-xivnsldf 192 13 of of IN cord-008590-xivnsldf 192 14 the the DT cord-008590-xivnsldf 192 15 principle principle JJ cord-008590-xivnsldf 192 16 glycoproteins glycoprotein NNS cord-008590-xivnsldf 192 17 and and CC cord-008590-xivnsldf 192 18 glycolipids glycolipid NNS cord-008590-xivnsldf 192 19 of of IN cord-008590-xivnsldf 192 20 the the DT cord-008590-xivnsldf 192 21 cells cell NNS cord-008590-xivnsldf 192 22 studied study VBD cord-008590-xivnsldf 192 23 . . . cord-008590-xivnsldf 193 1 Especially especially RB cord-008590-xivnsldf 193 2 in in IN cord-008590-xivnsldf 193 3 the the DT cord-008590-xivnsldf 193 4 case case NN cord-008590-xivnsldf 193 5 of of IN cord-008590-xivnsldf 193 6 the the DT cord-008590-xivnsldf 193 7 IgM IgM NNP cord-008590-xivnsldf 193 8 myeloma myeloma NNP cord-008590-xivnsldf 193 9 ( ( -LRB- cord-008590-xivnsldf 193 10 and and CC cord-008590-xivnsldf 193 11 to to IN cord-008590-xivnsldf 193 12 a a DT cord-008590-xivnsldf 193 13 lesser less JJR cord-008590-xivnsldf 193 14 extent extent NN cord-008590-xivnsldf 193 15 with with IN cord-008590-xivnsldf 193 16 the the DT cord-008590-xivnsldf 193 17 BHK BHK NNP cord-008590-xivnsldf 193 18 cells cell NNS cord-008590-xivnsldf 193 19 when when WRB cord-008590-xivnsldf 193 20 they -PRON- PRP cord-008590-xivnsldf 193 21 are be VBP cord-008590-xivnsldf 193 22 infected infect VBN cord-008590-xivnsldf 193 23 with with IN cord-008590-xivnsldf 193 24 Semliki Semliki NNP cord-008590-xivnsldf 193 25 Forest Forest NNP cord-008590-xivnsldf 193 26 virus virus NN cord-008590-xivnsldf 193 27 ) ) -RRB- cord-008590-xivnsldf 194 1 one one PRP cord-008590-xivnsldf 194 2 has have VBZ cord-008590-xivnsldf 194 3 reason reason NN cord-008590-xivnsldf 194 4 to to TO cord-008590-xivnsldf 194 5 consider consider VB cord-008590-xivnsldf 194 6 much much JJ cord-008590-xivnsldf 194 7 of of IN cord-008590-xivnsldf 194 8 the the DT cord-008590-xivnsldf 194 9 staining staining NN cord-008590-xivnsldf 194 10 to to TO cord-008590-xivnsldf 194 11 be be VB cord-008590-xivnsldf 194 12 due due JJ cord-008590-xivnsldf 194 13 to to IN cord-008590-xivnsldf 194 14 a a DT cord-008590-xivnsldf 194 15 single single JJ cord-008590-xivnsldf 194 16 glycoprotein glycoprotein NN cord-008590-xivnsldf 194 17 . . . cord-008590-xivnsldf 195 1 Nevertheless nevertheless RB cord-008590-xivnsldf 195 2 , , , cord-008590-xivnsldf 195 3 the the DT cord-008590-xivnsldf 195 4 study study NN cord-008590-xivnsldf 195 5 of of IN cord-008590-xivnsldf 195 6 the the DT cord-008590-xivnsldf 195 7 myeloma myeloma NN cord-008590-xivnsldf 195 8 mutant mutant NN cord-008590-xivnsldf 195 9 suggests suggest VBZ cord-008590-xivnsldf 195 10 that that IN cord-008590-xivnsldf 195 11 the the DT cord-008590-xivnsldf 195 12 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 195 13 of of IN cord-008590-xivnsldf 195 14 underlying underlie VBG cord-008590-xivnsldf 195 15 Golgi Golgi NNP cord-008590-xivnsldf 195 16 components component NNS cord-008590-xivnsldf 195 17 distribute distribute VBP cord-008590-xivnsldf 195 18 in in IN cord-008590-xivnsldf 195 19 roughly roughly RB cord-008590-xivnsldf 195 20 the the DT cord-008590-xivnsldf 195 21 same same JJ cord-008590-xivnsldf 195 22 way way NN cord-008590-xivnsldf 195 23 as as IN cord-008590-xivnsldf 195 24 the the DT cord-008590-xivnsldf 195 25 Ig Ig NNP cord-008590-xivnsldf 195 26 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 195 27 . . . cord-008590-xivnsldf 196 1 Taken take VBN cord-008590-xivnsldf 196 2 as as IN cord-008590-xivnsldf 196 3 a a DT cord-008590-xivnsldf 196 4 whole whole NN cord-008590-xivnsldf 196 5 , , , cord-008590-xivnsldf 196 6 the the DT cord-008590-xivnsldf 196 7 cytologic cytologic JJ cord-008590-xivnsldf 196 8 observations observation NNS cord-008590-xivnsldf 196 9 argue argue VBP cord-008590-xivnsldf 196 10 strongly strongly RB cord-008590-xivnsldf 196 11 that that IN cord-008590-xivnsldf 196 12 the the DT cord-008590-xivnsldf 196 13 direction direction NN cord-008590-xivnsldf 196 14 of of IN cord-008590-xivnsldf 196 15 transport transport NN cord-008590-xivnsldf 196 16 is be VBZ cord-008590-xivnsldf 196 17 indeed indeed RB cord-008590-xivnsldf 196 18 proximal proximal JJ cord-008590-xivnsldf 196 19 - - HYPH cord-008590-xivnsldf 196 20 todistal todistal NN cord-008590-xivnsldf 196 21 and and CC cord-008590-xivnsldf 196 22 that that IN cord-008590-xivnsldf 196 23 the the DT cord-008590-xivnsldf 196 24 terminal terminal JJ cord-008590-xivnsldf 196 25 sugars sugar NNS cord-008590-xivnsldf 196 26 are be VBP cord-008590-xivnsldf 196 27 acquired acquire VBN cord-008590-xivnsldf 196 28 during during IN cord-008590-xivnsldf 196 29 passage passage NN cord-008590-xivnsldf 196 30 across across IN cord-008590-xivnsldf 196 31 the the DT cord-008590-xivnsldf 196 32 stack stack NN cord-008590-xivnsldf 196 33 of of IN cord-008590-xivnsldf 196 34 Golgi Golgi NNP cord-008590-xivnsldf 196 35 cisternae cisternae NNP cord-008590-xivnsldf 196 36 . . . cord-008590-xivnsldf 197 1 These these DT cord-008590-xivnsldf 197 2 conclusions conclusion NNS cord-008590-xivnsldf 197 3 are be VBP cord-008590-xivnsldf 197 4 also also RB cord-008590-xivnsldf 197 5 compatible compatible JJ cord-008590-xivnsldf 197 6 with with IN cord-008590-xivnsldf 197 7 the the DT cord-008590-xivnsldf 197 8 available available JJ cord-008590-xivnsldf 197 9 monensin monensin NNP cord-008590-xivnsldf 197 10 data datum NNS cord-008590-xivnsldf 197 11 . . . cord-008590-xivnsldf 198 1 In in IN cord-008590-xivnsldf 198 2 short short JJ cord-008590-xivnsldf 198 3 - - HYPH cord-008590-xivnsldf 198 4 term term NN cord-008590-xivnsldf 198 5 experiments experiment NNS cord-008590-xivnsldf 198 6 with with IN cord-008590-xivnsldf 198 7 IgM IgM NNP cord-008590-xivnsldf 198 8 - - HYPH cord-008590-xivnsldf 198 9 secreting secrete VBG cord-008590-xivnsldf 198 10 plasma plasma NN cord-008590-xivnsldf 198 11 cells cell NNS cord-008590-xivnsldf 198 12 and and CC cord-008590-xivnsldf 198 13 myeloma myeloma NN cord-008590-xivnsldf 198 14 cells cell NNS cord-008590-xivnsldf 198 15 , , , cord-008590-xivnsldf 198 16 monensin monensin NNP cord-008590-xivnsldf 198 17 interrupts interrupt VBZ cord-008590-xivnsldf 198 18 ICT ICT NNP cord-008590-xivnsldf 198 19 within within IN cord-008590-xivnsldf 198 20 the the DT cord-008590-xivnsldf 198 21 GC GC NNP cord-008590-xivnsldf 198 22 at at IN cord-008590-xivnsldf 198 23 a a DT cord-008590-xivnsldf 198 24 site site NN cord-008590-xivnsldf 198 25 where where WRB cord-008590-xivnsldf 198 26 the the DT cord-008590-xivnsldf 198 27 Ig Ig NNP cord-008590-xivnsldf 198 28 heavy heavy JJ cord-008590-xivnsldf 198 29 chains chain NNS cord-008590-xivnsldf 198 30 are be VBP cord-008590-xivnsldf 198 31 still still RB cord-008590-xivnsldf 198 32 sensitive sensitive JJ cord-008590-xivnsldf 198 33 to to IN cord-008590-xivnsldf 198 34 endo endo FW cord-008590-xivnsldf 198 35 H h NN cord-008590-xivnsldf 198 36 and and CC cord-008590-xivnsldf 198 37 have have VBP cord-008590-xivnsldf 198 38 acquired acquire VBN cord-008590-xivnsldf 198 39 neither neither CC cord-008590-xivnsldf 198 40 galactose galactose NN cord-008590-xivnsldf 198 41 , , , cord-008590-xivnsldf 198 42 fucose fucose NNP cord-008590-xivnsldf 198 43 , , , cord-008590-xivnsldf 198 44 nor nor CC cord-008590-xivnsldf 198 45 sialic sialic JJ cord-008590-xivnsldf 198 46 acid acid NN cord-008590-xivnsldf 198 47 ( ( -LRB- cord-008590-xivnsldf 198 48 134 134 CD cord-008590-xivnsldf 198 49 , , , cord-008590-xivnsldf 198 50 135 135 CD cord-008590-xivnsldf 198 51 ) ) -RRB- cord-008590-xivnsldf 198 52 . . . cord-008590-xivnsldf 199 1 Terminal terminal JJ cord-008590-xivnsldf 199 2 sugar sugar NN cord-008590-xivnsldf 199 3 addition addition NN cord-008590-xivnsldf 199 4 to to IN cord-008590-xivnsldf 199 5 other other JJ cord-008590-xivnsldf 199 6 glycoproteins glycoprotein NNS cord-008590-xivnsldf 199 7 continues continue VBZ cord-008590-xivnsldf 199 8 and and CC cord-008590-xivnsldf 199 9 Ig Ig NNP cord-008590-xivnsldf 199 10 ( ( -LRB- cord-008590-xivnsldf 199 11 139 139 CD cord-008590-xivnsldf 199 12 ) ) -RRB- cord-008590-xivnsldf 199 13 . . . cord-008590-xivnsldf 200 1 Similar similar JJ cord-008590-xivnsldf 200 2 results result NNS cord-008590-xivnsldf 200 3 have have VBP cord-008590-xivnsldf 200 4 been be VBN cord-008590-xivnsldf 200 5 reported report VBN cord-008590-xivnsldf 200 6 for for IN cord-008590-xivnsldf 200 7 the the DT cord-008590-xivnsldf 200 8 effect effect NN cord-008590-xivnsldf 200 9 of of IN cord-008590-xivnsldf 200 10 monensin monensin NNP cord-008590-xivnsldf 200 11 on on IN cord-008590-xivnsldf 200 12 maturation maturation NN cord-008590-xivnsldf 200 13 of of IN cord-008590-xivnsldf 200 14 the the DT cord-008590-xivnsldf 200 15 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 200 16 of of IN cord-008590-xivnsldf 200 17 transfernin transfernin NNS cord-008590-xivnsldf 200 18 ( ( -LRB- cord-008590-xivnsldf 200 19 133 133 CD cord-008590-xivnsldf 200 20 ) ) -RRB- cord-008590-xivnsldf 200 21 and and CC cord-008590-xivnsldf 200 22 of of IN cord-008590-xivnsldf 200 23 the the DT cord-008590-xivnsldf 200 24 surface surface NN cord-008590-xivnsldf 200 25 glycoproteins glycoprotein NNS cord-008590-xivnsldf 200 26 of of IN cord-008590-xivnsldf 200 27 Semliki Semliki NNP cord-008590-xivnsldf 200 28 Forest Forest NNP cord-008590-xivnsldf 200 29 virus virus NN cord-008590-xivnsldf 200 30 ( ( -LRB- cord-008590-xivnsldf 200 31 37 37 CD cord-008590-xivnsldf 200 32 ) ) -RRB- cord-008590-xivnsldf 200 33 . . . cord-008590-xivnsldf 201 1 In in IN cord-008590-xivnsldf 201 2 other other JJ cord-008590-xivnsldf 201 3 cells cell NNS cord-008590-xivnsldf 201 4 and and CC cord-008590-xivnsldf 201 5 employing employ VBG cord-008590-xivnsldf 201 6 somewhat somewhat RB cord-008590-xivnsldf 201 7 different different JJ cord-008590-xivnsldf 201 8 protocols protocol NNS cord-008590-xivnsldf 201 9 the the DT cord-008590-xivnsldf 201 10 major major JJ cord-008590-xivnsldf 201 11 intra intra JJ cord-008590-xivnsldf 201 12 - - JJ cord-008590-xivnsldf 201 13 Golgi Golgi NNP cord-008590-xivnsldf 201 14 site site NN cord-008590-xivnsldf 201 15 of of IN cord-008590-xivnsldf 201 16 accumulation accumulation NN cord-008590-xivnsldf 201 17 of of IN cord-008590-xivnsldf 201 18 membrane membrane NN cord-008590-xivnsldf 201 19 proteins protein NNS cord-008590-xivnsldf 201 20 in in IN cord-008590-xivnsldf 201 21 the the DT cord-008590-xivnsldf 201 22 presence presence NN cord-008590-xivnsldf 201 23 of of IN cord-008590-xivnsldf 201 24 monensin monensin NNP cord-008590-xivnsldf 201 25 may may MD cord-008590-xivnsldf 201 26 be be VB cord-008590-xivnsldf 201 27 such such JJ cord-008590-xivnsldf 201 28 as as IN cord-008590-xivnsldf 201 29 to to TO cord-008590-xivnsldf 201 30 have have VB cord-008590-xivnsldf 201 31 allowed allow VBN cord-008590-xivnsldf 201 32 somewhat somewhat RB cord-008590-xivnsldf 201 33 further further JJ cord-008590-xivnsldf 201 34 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 201 35 maturation maturation NN cord-008590-xivnsldf 201 36 ( ( -LRB- cord-008590-xivnsldf 201 37 Table table NN cord-008590-xivnsldf 201 38 I i CD cord-008590-xivnsldf 201 39 ) ) -RRB- cord-008590-xivnsldf 201 40 . . . cord-008590-xivnsldf 202 1 Nevertheless nevertheless RB cord-008590-xivnsldf 202 2 , , , cord-008590-xivnsldf 202 3 transport transport NN cord-008590-xivnsldf 202 4 to to IN cord-008590-xivnsldf 202 5 the the DT cord-008590-xivnsldf 202 6 cell cell NN cord-008590-xivnsldf 202 7 surface surface NN cord-008590-xivnsldf 202 8 is be VBZ cord-008590-xivnsldf 202 9 not not RB cord-008590-xivnsldf 202 10 observed observe VBN cord-008590-xivnsldf 202 11 . . . cord-008590-xivnsldf 203 1 There there EX cord-008590-xivnsldf 203 2 is be VBZ cord-008590-xivnsldf 203 3 a a DT cord-008590-xivnsldf 203 4 line line NN cord-008590-xivnsldf 203 5 of of IN cord-008590-xivnsldf 203 6 experimentation experimentation NN cord-008590-xivnsldf 203 7 indicating indicate VBG cord-008590-xivnsldf 203 8 the the DT cord-008590-xivnsldf 203 9 existence existence NN cord-008590-xivnsldf 203 10 of of IN cord-008590-xivnsldf 203 11 two two CD cord-008590-xivnsldf 203 12 sequential sequential JJ cord-008590-xivnsldf 203 13 subcompartments subcompartment NNS cord-008590-xivnsldf 203 14 traversed traverse VBN cord-008590-xivnsldf 203 15 by by IN cord-008590-xivnsldf 203 16 the the DT cord-008590-xivnsldf 203 17 G g NN cord-008590-xivnsldf 203 18 protein protein NN cord-008590-xivnsldf 203 19 of of IN cord-008590-xivnsldf 203 20 VSV VSV NNP cord-008590-xivnsldf 203 21 . . . cord-008590-xivnsldf 204 1 The the DT cord-008590-xivnsldf 204 2 ultimate ultimate JJ cord-008590-xivnsldf 204 3 interpretation interpretation NN cord-008590-xivnsldf 204 4 of of IN cord-008590-xivnsldf 204 5 the the DT cord-008590-xivnsldf 204 6 data data NN cord-008590-xivnsldf 204 7 remains remain VBZ cord-008590-xivnsldf 204 8 to to TO cord-008590-xivnsldf 204 9 be be VB cord-008590-xivnsldf 204 10 determined determine VBN cord-008590-xivnsldf 204 11 , , , cord-008590-xivnsldf 204 12 but but CC cord-008590-xivnsldf 204 13 is be VBZ cord-008590-xivnsldf 204 14 thought think VBN cord-008590-xivnsldf 204 15 to to TO cord-008590-xivnsldf 204 16 pertain pertain VB cord-008590-xivnsldf 204 17 to to IN cord-008590-xivnsldf 204 18 the the DT cord-008590-xivnsldf 204 19 ability ability NN cord-008590-xivnsldf 204 20 of of IN cord-008590-xivnsldf 204 21 the the DT cord-008590-xivnsldf 204 22 membranes membrane NNS cord-008590-xivnsldf 204 23 of of IN cord-008590-xivnsldf 204 24 Golgi Golgi NNP cord-008590-xivnsldf 204 25 subfractions subfraction NNS cord-008590-xivnsldf 204 26 to to TO cord-008590-xivnsldf 204 27 undergo undergo VB cord-008590-xivnsldf 204 28 fusion fusion NN cord-008590-xivnsldf 204 29 with with IN cord-008590-xivnsldf 204 30 the the DT cord-008590-xivnsldf 204 31 membrane membrane NN cord-008590-xivnsldf 204 32 of of IN cord-008590-xivnsldf 204 33 a a DT cord-008590-xivnsldf 204 34 relatively relatively RB cord-008590-xivnsldf 204 35 late late JJ cord-008590-xivnsldf 204 36 subcompartment subcompartment NN cord-008590-xivnsldf 204 37 . . . cord-008590-xivnsldf 205 1 In in IN cord-008590-xivnsldf 205 2 the the DT cord-008590-xivnsldf 205 3 protocols protocol NNS cord-008590-xivnsldf 205 4 employed employ VBN cord-008590-xivnsldf 205 5 ( ( -LRB- cord-008590-xivnsldf 205 6 20 20 CD cord-008590-xivnsldf 205 7 , , , cord-008590-xivnsldf 205 8 30a 30a NNS cord-008590-xivnsldf 205 9 , , , cord-008590-xivnsldf 205 10 11 11 CD cord-008590-xivnsldf 205 11 1 1 CD cord-008590-xivnsldf 205 12 ) ) -RRB- cord-008590-xivnsldf 205 13 VSVinfected vsvinfecte VBN cord-008590-xivnsldf 205 14 mutant mutant JJ cord-008590-xivnsldf 205 15 CHO CHO NNP cord-008590-xivnsldf 205 16 cells cell NNS cord-008590-xivnsldf 205 17 ( ( -LRB- cord-008590-xivnsldf 205 18 lacking lack VBG cord-008590-xivnsldf 205 19 GlcNAc glcnac NN cord-008590-xivnsldf 205 20 transferase transferase NN cord-008590-xivnsldf 205 21 I I NNP cord-008590-xivnsldf 205 22 ) ) -RRB- cord-008590-xivnsldf 205 23 are be VBP cord-008590-xivnsldf 205 24 pulse pulse NN cord-008590-xivnsldf 205 25 - - HYPH cord-008590-xivnsldf 205 26 labeled label VBN cord-008590-xivnsldf 205 27 with with IN cord-008590-xivnsldf 205 28 [ [ -LRB- cord-008590-xivnsldf 205 29 35S]methionine 35s]methionine CD cord-008590-xivnsldf 205 30 and and CC cord-008590-xivnsldf 205 31 chased chase VBN cord-008590-xivnsldf 205 32 for for IN cord-008590-xivnsldf 205 33 increasing increase VBG cord-008590-xivnsldf 205 34 intervals interval NNS cord-008590-xivnsldf 205 35 . . . cord-008590-xivnsldf 206 1 At at IN cord-008590-xivnsldf 206 2 each each DT cord-008590-xivnsldf 206 3 time time NN cord-008590-xivnsldf 206 4 point point NN cord-008590-xivnsldf 206 5 a a DT cord-008590-xivnsldf 206 6 cell cell NN cord-008590-xivnsldf 206 7 hornogenate hornogenate NN cord-008590-xivnsldf 206 8 is be VBZ cord-008590-xivnsldf 206 9 mixed mix VBN cord-008590-xivnsldf 206 10 with with IN cord-008590-xivnsldf 206 11 a a DT cord-008590-xivnsldf 206 12 wild wild JJ cord-008590-xivnsldf 206 13 - - HYPH cord-008590-xivnsldf 206 14 type type NN cord-008590-xivnsldf 206 15 homogenate homogenate NN cord-008590-xivnsldf 206 16 , , , cord-008590-xivnsldf 206 17 briefly briefly NN cord-008590-xivnsldf 206 18 incubated incubate VBN cord-008590-xivnsldf 206 19 , , , cord-008590-xivnsldf 206 20 and and CC cord-008590-xivnsldf 206 21 assayed assay VBN cord-008590-xivnsldf 206 22 for for IN cord-008590-xivnsldf 206 23 the the DT cord-008590-xivnsldf 206 24 proportion proportion NN cord-008590-xivnsldf 206 25 of of IN cord-008590-xivnsldf 206 26 35S 35S NNP cord-008590-xivnsldf 206 27 - - HYPH cord-008590-xivnsldf 206 28 labeled label VBN cord-008590-xivnsldf 206 29 G g NN cord-008590-xivnsldf 206 30 ( ( -LRB- cord-008590-xivnsldf 206 31 initially initially RB cord-008590-xivnsldf 206 32 sensitive sensitive JJ cord-008590-xivnsldf 206 33 to to IN cord-008590-xivnsldf 206 34 endo endo FW cord-008590-xivnsldf 206 35 H h NN cord-008590-xivnsldf 206 36 and and CC cord-008590-xivnsldf 206 37 perpetually perpetually RB cord-008590-xivnsldf 206 38 so so RB cord-008590-xivnsldf 206 39 in in IN cord-008590-xivnsldf 206 40 the the DT cord-008590-xivnsldf 206 41 mutant mutant JJ cord-008590-xivnsldf 206 42 CHO CHO NNP cord-008590-xivnsldf 206 43 cell cell NN cord-008590-xivnsldf 206 44 ) ) -RRB- cord-008590-xivnsldf 206 45 which which WDT cord-008590-xivnsldf 206 46 has have VBZ cord-008590-xivnsldf 206 47 acquired acquire VBN cord-008590-xivnsldf 206 48 resistance resistance NN cord-008590-xivnsldf 206 49 to to IN cord-008590-xivnsldf 206 50 endo endo NN cord-008590-xivnsldf 206 51 H. H. NNP cord-008590-xivnsldf 207 1 The the DT cord-008590-xivnsldf 207 2 in in IN cord-008590-xivnsldf 207 3 virro virro NN cord-008590-xivnsldf 207 4 maturation maturation NN cord-008590-xivnsldf 207 5 is be VBZ cord-008590-xivnsldf 207 6 manifest manifest VBG cord-008590-xivnsldf 207 7 only only RB cord-008590-xivnsldf 207 8 after after IN cord-008590-xivnsldf 207 9 a a DT cord-008590-xivnsldf 207 10 brief brief JJ cord-008590-xivnsldf 207 11 chase chase NN cord-008590-xivnsldf 207 12 interval interval NN cord-008590-xivnsldf 207 13 , , , cord-008590-xivnsldf 207 14 suggesting suggest VBG cord-008590-xivnsldf 207 15 that that IN cord-008590-xivnsldf 207 16 at at IN cord-008590-xivnsldf 207 17 later later RB cord-008590-xivnsldf 207 18 times time NNS cord-008590-xivnsldf 207 19 G G NNP cord-008590-xivnsldf 207 20 has have VBZ cord-008590-xivnsldf 207 21 gained gain VBN cord-008590-xivnsldf 207 22 access access NN cord-008590-xivnsldf 207 23 to to IN cord-008590-xivnsldf 207 24 a a DT cord-008590-xivnsldf 207 25 subcompartment subcompartment NN cord-008590-xivnsldf 207 26 which which WDT cord-008590-xivnsldf 207 27 will will MD cord-008590-xivnsldf 207 28 no no RB cord-008590-xivnsldf 207 29 longer longer RB cord-008590-xivnsldf 207 30 participate participate VB cord-008590-xivnsldf 207 31 in in IN cord-008590-xivnsldf 207 32 such such JJ cord-008590-xivnsldf 207 33 membrane membrane NN cord-008590-xivnsldf 207 34 - - HYPH cord-008590-xivnsldf 207 35 membrane membrane NN cord-008590-xivnsldf 207 36 fusion fusion NN cord-008590-xivnsldf 207 37 . . . cord-008590-xivnsldf 208 1 Numerous numerous JJ cord-008590-xivnsldf 208 2 yeast yeast NN cord-008590-xivnsldf 208 3 glycoproteins glycoprotein NNS cord-008590-xivnsldf 208 4 , , , cord-008590-xivnsldf 208 5 for for IN cord-008590-xivnsldf 208 6 example example NN cord-008590-xivnsldf 208 7 , , , cord-008590-xivnsldf 208 8 secretory secretory JJ cord-008590-xivnsldf 208 9 invertase invertase NN cord-008590-xivnsldf 208 10 and and CC cord-008590-xivnsldf 208 11 acid acid NN cord-008590-xivnsldf 208 12 phosphatase phosphatase NN cord-008590-xivnsldf 208 13 , , , cord-008590-xivnsldf 208 14 bear bear VB cord-008590-xivnsldf 208 15 both both DT cord-008590-xivnsldf 208 16 asparagine asparagine NN cord-008590-xivnsldf 208 17 - - HYPH cord-008590-xivnsldf 208 18 linked link VBN cord-008590-xivnsldf 208 19 and and CC cord-008590-xivnsldf 208 20 0-linked 0-linked CD cord-008590-xivnsldf 208 21 polymannosyl polymannosyl NN cord-008590-xivnsldf 208 22 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 208 23 . . . cord-008590-xivnsldf 209 1 The the DT cord-008590-xivnsldf 209 2 asparagine asparagine NN cord-008590-xivnsldf 209 3 - - HYPH cord-008590-xivnsldf 209 4 linked link VBN cord-008590-xivnsldf 209 5 units unit NNS cord-008590-xivnsldf 209 6 are be VBP cord-008590-xivnsldf 209 7 donated donate VBN cord-008590-xivnsldf 209 8 from from IN cord-008590-xivnsldf 209 9 the the DT cord-008590-xivnsldf 209 10 same same JJ cord-008590-xivnsldf 209 11 dolichol dolichol NN cord-008590-xivnsldf 209 12 - - HYPH cord-008590-xivnsldf 209 13 linked link VBN cord-008590-xivnsldf 209 14 intermediates intermediate NNS cord-008590-xivnsldf 209 15 as as IN cord-008590-xivnsldf 209 16 in in IN cord-008590-xivnsldf 209 17 mammalian mammalian JJ cord-008590-xivnsldf 209 18 cells cell NNS cord-008590-xivnsldf 209 19 , , , cord-008590-xivnsldf 209 20 trimmed trim VBN cord-008590-xivnsldf 209 21 to to IN cord-008590-xivnsldf 209 22 a a DT cord-008590-xivnsldf 209 23 structure structure NN cord-008590-xivnsldf 209 24 containing contain VBG cord-008590-xivnsldf 209 25 2 2 CD cord-008590-xivnsldf 209 26 GlcNAc GlcNAc NNP cord-008590-xivnsldf 209 27 and and CC cord-008590-xivnsldf 209 28 8 8 CD cord-008590-xivnsldf 209 29 Man man NN cord-008590-xivnsldf 209 30 , , , cord-008590-xivnsldf 209 31 and and CC cord-008590-xivnsldf 209 32 elongated elongate VBN cord-008590-xivnsldf 209 33 by by IN cord-008590-xivnsldf 209 34 massive massive JJ cord-008590-xivnsldf 209 35 further further JJ cord-008590-xivnsldf 209 36 addition addition NN cord-008590-xivnsldf 209 37 of of IN cord-008590-xivnsldf 209 38 mannose mannose NN cord-008590-xivnsldf 209 39 and and CC cord-008590-xivnsldf 209 40 phosphate phosphate NN cord-008590-xivnsldf 209 41 ( ( -LRB- cord-008590-xivnsldf 209 42 3 3 CD cord-008590-xivnsldf 209 43 ) ) -RRB- cord-008590-xivnsldf 209 44 . . . cord-008590-xivnsldf 210 1 Studies study NNS cord-008590-xivnsldf 210 2 of of IN cord-008590-xivnsldf 210 3 yeast yeast NN cord-008590-xivnsldf 210 4 temperature temperature NN cord-008590-xivnsldf 210 5 - - HYPH cord-008590-xivnsldf 210 6 sensitive sensitive JJ cord-008590-xivnsldf 210 7 ICT ICT NNP cord-008590-xivnsldf 210 8 mutants mutant NNS cord-008590-xivnsldf 210 9 which which WDT cord-008590-xivnsldf 210 10 overaccumulate overaccumulate VBP cord-008590-xivnsldf 210 11 RER RER NNP cord-008590-xivnsldf 210 12 , , , cord-008590-xivnsldf 210 13 Golgi Golgi NNP cord-008590-xivnsldf 210 14 - - HYPH cord-008590-xivnsldf 210 15 like like JJ cord-008590-xivnsldf 210 16 cisternae cisternae NN cord-008590-xivnsldf 210 17 , , , cord-008590-xivnsldf 210 18 or or CC cord-008590-xivnsldf 210 19 vesicles vesicle NNS cord-008590-xivnsldf 210 20 suggest suggest VBP cord-008590-xivnsldf 210 21 that that IN cord-008590-xivnsldf 210 22 the the DT cord-008590-xivnsldf 210 23 polymannosyl polymannosyl NN cord-008590-xivnsldf 210 24 elongation elongation NN cord-008590-xivnsldf 210 25 of of IN cord-008590-xivnsldf 210 26 the the DT cord-008590-xivnsldf 210 27 N n NN cord-008590-xivnsldf 210 28 - - HYPH cord-008590-xivnsldf 210 29 linked link VBN cord-008590-xivnsldf 210 30 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 210 31 of of IN cord-008590-xivnsldf 210 32 invertase invertase NN cord-008590-xivnsldf 210 33 occurs occur VBZ cord-008590-xivnsldf 210 34 within within IN cord-008590-xivnsldf 210 35 the the DT cord-008590-xivnsldf 210 36 GC GC NNP cord-008590-xivnsldf 210 37 ( ( -LRB- cord-008590-xivnsldf 210 38 21 21 CD cord-008590-xivnsldf 210 39 ) ) -RRB- cord-008590-xivnsldf 210 40 . . . cord-008590-xivnsldf 211 1 The the DT cord-008590-xivnsldf 211 2 phosphorylation phosphorylation NN cord-008590-xivnsldf 211 3 of of IN cord-008590-xivnsldf 211 4 the the DT cord-008590-xivnsldf 211 5 secretory secretory JJ cord-008590-xivnsldf 211 6 protein protein NN cord-008590-xivnsldf 211 7 also also RB cord-008590-xivnsldf 211 8 occurs occur VBZ cord-008590-xivnsldf 211 9 within within IN cord-008590-xivnsldf 211 10 this this DT cord-008590-xivnsldf 211 11 compartment compartment NN cord-008590-xivnsldf 211 12 ( ( -LRB- cord-008590-xivnsldf 211 13 13 13 CD cord-008590-xivnsldf 211 14 1 1 CD cord-008590-xivnsldf 211 15 ) ) -RRB- cord-008590-xivnsldf 211 16 . . . cord-008590-xivnsldf 212 1 ( ( -LRB- cord-008590-xivnsldf 212 2 71 71 CD cord-008590-xivnsldf 212 3 , , , cord-008590-xivnsldf 212 4 86 86 CD cord-008590-xivnsldf 212 5 , , , cord-008590-xivnsldf 212 6 124 124 CD cord-008590-xivnsldf 212 7 ) ) -RRB- cord-008590-xivnsldf 213 1 In in IN cord-008590-xivnsldf 213 2 cultured cultured JJ cord-008590-xivnsldf 213 3 fibroblasts fibroblast NNS cord-008590-xivnsldf 213 4 , , , cord-008590-xivnsldf 213 5 the the DT cord-008590-xivnsldf 213 6 proper proper JJ cord-008590-xivnsldf 213 7 intracellular intracellular JJ cord-008590-xivnsldf 213 8 targeting targeting NN cord-008590-xivnsldf 213 9 of of IN cord-008590-xivnsldf 213 10 lysosomal lysosomal JJ cord-008590-xivnsldf 213 11 enzymes enzyme NNS cord-008590-xivnsldf 213 12 depends depend VBZ cord-008590-xivnsldf 213 13 on on IN cord-008590-xivnsldf 213 14 the the DT cord-008590-xivnsldf 213 15 presence presence NN cord-008590-xivnsldf 213 16 of of IN cord-008590-xivnsldf 213 17 mannose mannose VBG cord-008590-xivnsldf 213 18 6-phosphate 6-phosphate NN cord-008590-xivnsldf 213 19 on on IN cord-008590-xivnsldf 213 20 high high JJ cord-008590-xivnsldf 213 21 mannose mannose NN cord-008590-xivnsldf 213 22 asparaginelinked asparaginelinke VBN cord-008590-xivnsldf 213 23 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 213 24 . . . cord-008590-xivnsldf 214 1 The the DT cord-008590-xivnsldf 214 2 phosphorylated phosphorylated JJ cord-008590-xivnsldf 214 3 structure structure NN cord-008590-xivnsldf 214 4 is be VBZ cord-008590-xivnsldf 214 5 the the DT cord-008590-xivnsldf 214 6 result result NN cord-008590-xivnsldf 214 7 of of IN cord-008590-xivnsldf 214 8 the the DT cord-008590-xivnsldf 214 9 posttranslational posttranslational JJ cord-008590-xivnsldf 214 10 addition addition NN cord-008590-xivnsldf 214 11 of of IN cord-008590-xivnsldf 214 12 one one CD cord-008590-xivnsldf 214 13 or or CC cord-008590-xivnsldf 214 14 several several JJ cord-008590-xivnsldf 214 15 GlcNAc-1 GlcNAc-1 NNS cord-008590-xivnsldf 214 16 -phosphate -phosphate : cord-008590-xivnsldf 214 17 units unit NNS cord-008590-xivnsldf 214 18 to to IN cord-008590-xivnsldf 214 19 several several JJ cord-008590-xivnsldf 214 20 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 214 21 per per IN cord-008590-xivnsldf 214 22 polypeptide polypeptide NN cord-008590-xivnsldf 214 23 , , , cord-008590-xivnsldf 214 24 followed follow VBN cord-008590-xivnsldf 214 25 by by IN cord-008590-xivnsldf 214 26 removal removal NN cord-008590-xivnsldf 214 27 of of IN cord-008590-xivnsldf 214 28 much much JJ cord-008590-xivnsldf 214 29 of of IN cord-008590-xivnsldf 214 30 the the DT cord-008590-xivnsldf 214 31 GlcNAc GlcNAc NNP cord-008590-xivnsldf 214 32 . . . cord-008590-xivnsldf 215 1 Biosynthetic biosynthetic JJ cord-008590-xivnsldf 215 2 labeling labeling NN cord-008590-xivnsldf 215 3 studies study NNS cord-008590-xivnsldf 215 4 show show VBP cord-008590-xivnsldf 215 5 that that IN cord-008590-xivnsldf 215 6 the the DT cord-008590-xivnsldf 215 7 " " `` cord-008590-xivnsldf 215 8 unblocked unblocked JJ cord-008590-xivnsldf 215 9 " " '' cord-008590-xivnsldf 215 10 phosphate phosphate NN cord-008590-xivnsldf 215 11 structure structure NN cord-008590-xivnsldf 215 12 is be VBZ cord-008590-xivnsldf 215 13 achieved achieve VBN cord-008590-xivnsldf 215 14 within within IN cord-008590-xivnsldf 215 15 1 1 CD cord-008590-xivnsldf 215 16 hour hour NN cord-008590-xivnsldf 215 17 after after IN cord-008590-xivnsldf 215 18 a a DT cord-008590-xivnsldf 215 19 [ [ -LRB- cord-008590-xivnsldf 215 20 3H]mannose 3h]mannose CD cord-008590-xivnsldf 215 21 pulse pulse NN cord-008590-xivnsldf 215 22 and and CC cord-008590-xivnsldf 215 23 that that IN cord-008590-xivnsldf 215 24 phosphoryl phosphoryl NNS cord-008590-xivnsldf 215 25 groups group NNS cord-008590-xivnsldf 215 26 are be VBP cord-008590-xivnsldf 215 27 ultimately ultimately RB cord-008590-xivnsldf 215 28 lost lose VBN cord-008590-xivnsldf 215 29 , , , cord-008590-xivnsldf 215 30 presumably presumably RB cord-008590-xivnsldf 215 31 upon upon IN cord-008590-xivnsldf 215 32 entry entry NN cord-008590-xivnsldf 215 33 into into IN cord-008590-xivnsldf 215 34 lysosomes lysosome NNS cord-008590-xivnsldf 215 35 ( ( -LRB- cord-008590-xivnsldf 215 36 31 31 CD cord-008590-xivnsldf 215 37 ) ) -RRB- cord-008590-xivnsldf 215 38 . . . cord-008590-xivnsldf 216 1 The the DT cord-008590-xivnsldf 216 2 enzymes enzyme NNS cord-008590-xivnsldf 216 3 responsible responsible JJ cord-008590-xivnsldf 216 4 both both DT cord-008590-xivnsldf 216 5 for for IN cord-008590-xivnsldf 216 6 addition addition NN cord-008590-xivnsldf 216 7 of of IN cord-008590-xivnsldf 216 8 the the DT cord-008590-xivnsldf 216 9 blocked block VBN cord-008590-xivnsldf 216 10 phosphate phosphate NN cord-008590-xivnsldf 216 11 and and CC cord-008590-xivnsldf 216 12 subsequent subsequent JJ cord-008590-xivnsldf 216 13 removal removal NN cord-008590-xivnsldf 216 14 of of IN cord-008590-xivnsldf 216 15 the the DT cord-008590-xivnsldf 216 16 GlcNAc GlcNAc NNP cord-008590-xivnsldf 216 17 are be VBP cord-008590-xivnsldf 216 18 both both DT cord-008590-xivnsldf 216 19 enriched enrich VBN cord-008590-xivnsldf 216 20 in in IN cord-008590-xivnsldf 216 21 preparative preparative JJ cord-008590-xivnsldf 216 22 liver liver NN cord-008590-xivnsldf 216 23 Golgi Golgi NNP cord-008590-xivnsldf 216 24 fractions fraction NNS cord-008590-xivnsldf 216 25 . . . cord-008590-xivnsldf 217 1 In in IN cord-008590-xivnsldf 217 2 attempts attempt NNS cord-008590-xivnsldf 217 3 at at IN cord-008590-xivnsldf 217 4 liver liver NN cord-008590-xivnsldf 217 5 Golgi Golgi NNP cord-008590-xivnsldf 217 6 subfractionation subfractionation NN cord-008590-xivnsldf 217 7 , , , cord-008590-xivnsldf 217 8 the the DT cord-008590-xivnsldf 217 9 two two CD cord-008590-xivnsldf 217 10 activities activity NNS cord-008590-xivnsldf 217 11 have have VBP cord-008590-xivnsldf 217 12 been be VBN cord-008590-xivnsldf 217 13 reported report VBN cord-008590-xivnsldf 217 14 to to TO cord-008590-xivnsldf 217 15 be be VB cord-008590-xivnsldf 217 16 coisopycnic coisopycnic NNP cord-008590-xivnsldf 217 17 with with IN cord-008590-xivnsldf 217 18 mannosidase mannosidase NN cord-008590-xivnsldf 218 1 I -PRON- PRP cord-008590-xivnsldf 218 2 , , , cord-008590-xivnsldf 218 3 i.e. i.e. FW cord-008590-xivnsldf 218 4 , , , cord-008590-xivnsldf 218 5 denser dense JJR cord-008590-xivnsldf 218 6 than than IN cord-008590-xivnsldf 218 7 galactosyltransferase galactosyltransferase NNP cord-008590-xivnsldf 218 8 ( ( -LRB- cord-008590-xivnsldf 218 9 100 100 CD cord-008590-xivnsldf 218 10 ) ) -RRB- cord-008590-xivnsldf 218 11 . . . cord-008590-xivnsldf 219 1 Sucrose sucrose NN cord-008590-xivnsldf 219 2 gradient gradient NN cord-008590-xivnsldf 219 3 subfractionation subfractionation NN cord-008590-xivnsldf 219 4 of of IN cord-008590-xivnsldf 219 5 a a DT cord-008590-xivnsldf 219 6 mouse mouse NN cord-008590-xivnsldf 219 7 lymphoma lymphoma NN cord-008590-xivnsldf 219 8 and and CC cord-008590-xivnsldf 219 9 macrophage macrophage NN cord-008590-xivnsldf 219 10 cell cell NN cord-008590-xivnsldf 219 11 line line NN cord-008590-xivnsldf 219 12 has have VBZ cord-008590-xivnsldf 219 13 partially partially RB cord-008590-xivnsldf 219 14 resolved resolve VBN cord-008590-xivnsldf 219 15 the the DT cord-008590-xivnsldf 219 16 phosphotransferase phosphotransferase NN cord-008590-xivnsldf 219 17 , , , cord-008590-xivnsldf 219 18 phosphodiesterase phosphodiesterase NN cord-008590-xivnsldf 219 19 , , , cord-008590-xivnsldf 219 20 and and CC cord-008590-xivnsldf 219 21 Gal Gal NNP cord-008590-xivnsldf 219 22 transferase transferase NN cord-008590-xivnsldf 219 23 ( ( -LRB- cord-008590-xivnsldf 219 24 34a 34a NNS cord-008590-xivnsldf 219 25 ) ) -RRB- cord-008590-xivnsldf 219 26 . . . cord-008590-xivnsldf 220 1 There there EX cord-008590-xivnsldf 220 2 is be VBZ cord-008590-xivnsldf 220 3 also also RB cord-008590-xivnsldf 220 4 a a DT cord-008590-xivnsldf 220 5 further further JJ cord-008590-xivnsldf 220 6 suggestion suggestion NN cord-008590-xivnsldf 220 7 that that IN cord-008590-xivnsldf 220 8 mannosidase mannosidase NN cord-008590-xivnsldf 220 9 I I NNP cord-008590-xivnsldf 220 10 and and CC cord-008590-xivnsldf 220 11 the the DT cord-008590-xivnsldf 220 12 phosphotransferase phosphotransferase NNS cord-008590-xivnsldf 220 13 are be VBP cord-008590-xivnsldf 220 14 localized localize VBN cord-008590-xivnsldf 220 15 to to IN cord-008590-xivnsldf 220 16 the the DT cord-008590-xivnsldf 220 17 same same JJ cord-008590-xivnsldf 220 18 Golgi Golgi NNP cord-008590-xivnsldf 220 19 subcompartment subcompartment NN cord-008590-xivnsldf 220 20 - - HYPH cord-008590-xivnsldf 220 21 in in RP cord-008590-xivnsldf 220 22 3H 3h CD cord-008590-xivnsldf 220 23 - - HYPH cord-008590-xivnsldf 220 24 Man man NN cord-008590-xivnsldf 220 25 pulse pulse NN cord-008590-xivnsldf 220 26 - - HYPH cord-008590-xivnsldf 220 27 chase chase NN cord-008590-xivnsldf 220 28 studies study NNS cord-008590-xivnsldf 220 29 the the DT cord-008590-xivnsldf 220 30 degree degree NN cord-008590-xivnsldf 220 31 of of IN cord-008590-xivnsldf 220 32 trimming trim VBG cord-008590-xivnsldf 220 33 to to IN cord-008590-xivnsldf 220 34 a a DT cord-008590-xivnsldf 220 35 Man man NN cord-008590-xivnsldf 220 36 , , , cord-008590-xivnsldf 220 37 structure structure NN cord-008590-xivnsldf 220 38 correlates correlate VBZ cord-008590-xivnsldf 220 39 with with IN cord-008590-xivnsldf 220 40 the the DT cord-008590-xivnsldf 220 41 progressive progressive JJ cord-008590-xivnsldf 220 42 addition addition NN cord-008590-xivnsldf 220 43 of of IN cord-008590-xivnsldf 220 44 blocked block VBN cord-008590-xivnsldf 220 45 phosphates phosphate NNS cord-008590-xivnsldf 220 46 ( ( -LRB- cord-008590-xivnsldf 220 47 34 34 CD cord-008590-xivnsldf 220 48 ) ) -RRB- cord-008590-xivnsldf 220 49 . . . cord-008590-xivnsldf 221 1 Since since IN cord-008590-xivnsldf 221 2 lysosomal lysosomal JJ cord-008590-xivnsldf 221 3 enzymes enzyme NNS cord-008590-xivnsldf 221 4 also also RB cord-008590-xivnsldf 221 5 bear bear VBP cord-008590-xivnsldf 221 6 complex complex JJ cord-008590-xivnsldf 221 7 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 221 8 ( ( -LRB- cord-008590-xivnsldf 221 9 34 34 CD cord-008590-xivnsldf 221 10 , , , cord-008590-xivnsldf 221 11 45 45 CD cord-008590-xivnsldf 221 12 , , , cord-008590-xivnsldf 221 13 124 124 CD cord-008590-xivnsldf 221 14 ) ) -RRB- cord-008590-xivnsldf 221 15 , , , cord-008590-xivnsldf 221 16 they -PRON- PRP cord-008590-xivnsldf 221 17 must must MD cord-008590-xivnsldf 221 18 pass pass VB cord-008590-xivnsldf 221 19 via via IN cord-008590-xivnsldf 221 20 the the DT cord-008590-xivnsldf 221 21 terminal terminal JJ cord-008590-xivnsldf 221 22 sugar sugar NN cord-008590-xivnsldf 221 23 transferases transferase NNS cord-008590-xivnsldf 221 24 ( ( -LRB- cord-008590-xivnsldf 221 25 i.e. i.e. FW cord-008590-xivnsldf 221 26 , , , cord-008590-xivnsldf 221 27 anatomically anatomically RB cord-008590-xivnsldf 221 28 distal distal JJ cord-008590-xivnsldf 221 29 cisternae cisternae NNP cord-008590-xivnsldf 221 30 ) ) -RRB- cord-008590-xivnsldf 221 31 , , , cord-008590-xivnsldf 221 32 and and CC cord-008590-xivnsldf 221 33 indeed indeed RB cord-008590-xivnsldf 221 34 they -PRON- PRP cord-008590-xivnsldf 221 35 can can MD cord-008590-xivnsldf 221 36 be be VB cord-008590-xivnsldf 221 37 visualized visualize VBN cord-008590-xivnsldf 221 38 within within IN cord-008590-xivnsldf 221 39 all all DT cord-008590-xivnsldf 221 40 Golgi Golgi NNP cord-008590-xivnsldf 221 41 cisternae cisternae NNP cord-008590-xivnsldf 221 42 by by IN cord-008590-xivnsldf 221 43 histochemical histochemical JJ cord-008590-xivnsldf 221 44 methods method NNS cord-008590-xivnsldf 221 45 . . . cord-008590-xivnsldf 222 1 This this DT cord-008590-xivnsldf 222 2 route route NN cord-008590-xivnsldf 222 3 of of IN cord-008590-xivnsldf 222 4 passage passage NN cord-008590-xivnsldf 222 5 is be VBZ cord-008590-xivnsldf 222 6 acutely acutely RB cord-008590-xivnsldf 222 7 manifest manifest JJ cord-008590-xivnsldf 222 8 in in IN cord-008590-xivnsldf 222 9 the the DT cord-008590-xivnsldf 222 10 I i NN cord-008590-xivnsldf 222 11 - - HYPH cord-008590-xivnsldf 222 12 cell cell NN cord-008590-xivnsldf 222 13 syndrome syndrome NN cord-008590-xivnsldf 222 14 in in IN cord-008590-xivnsldf 222 15 which which WDT cord-008590-xivnsldf 222 16 GlcNAc-1 GlcNAc-1 NNS cord-008590-xivnsldf 222 17 -phosphotransferase -phosphotransferase : cord-008590-xivnsldf 222 18 activity activity NN cord-008590-xivnsldf 222 19 is be VBZ cord-008590-xivnsldf 222 20 missing miss VBG cord-008590-xivnsldf 222 21 and and CC cord-008590-xivnsldf 222 22 many many JJ cord-008590-xivnsldf 222 23 lysosomal lysosomal JJ cord-008590-xivnsldf 222 24 enzymes enzyme NNS cord-008590-xivnsldf 222 25 are be VBP cord-008590-xivnsldf 222 26 released release VBN cord-008590-xivnsldf 222 27 from from IN cord-008590-xivnsldf 222 28 cells cell NNS cord-008590-xivnsldf 222 29 . . . cord-008590-xivnsldf 223 1 Such such JJ cord-008590-xivnsldf 223 2 molecules molecule NNS cord-008590-xivnsldf 223 3 contain contain VBP cord-008590-xivnsldf 223 4 more more JJR cord-008590-xivnsldf 223 5 sialic sialic JJ cord-008590-xivnsldf 223 6 acid acid NN cord-008590-xivnsldf 223 7 and and CC cord-008590-xivnsldf 223 8 are be VBP cord-008590-xivnsldf 223 9 bound bind VBN cord-008590-xivnsldf 223 10 to to IN cord-008590-xivnsldf 223 11 insolubilized insolubilize VBD cord-008590-xivnsldf 223 12 rich rich JJ cord-008590-xivnsldf 223 13 to to IN cord-008590-xivnsldf 223 14 a a DT cord-008590-xivnsldf 223 15 greater great JJR cord-008590-xivnsldf 223 16 extent extent NN cord-008590-xivnsldf 223 17 than than IN cord-008590-xivnsldf 223 18 in in IN cord-008590-xivnsldf 223 19 the the DT cord-008590-xivnsldf 223 20 wild wild JJ cord-008590-xivnsldf 223 21 type type NN cord-008590-xivnsldf 223 22 ( ( -LRB- cord-008590-xivnsldf 223 23 124 124 CD cord-008590-xivnsldf 223 24 , , , cord-008590-xivnsldf 223 25 134a 134a NNP cord-008590-xivnsldf 223 26 ) ) -RRB- cord-008590-xivnsldf 223 27 . . . cord-008590-xivnsldf 224 1 A a DT cord-008590-xivnsldf 224 2 somewhat somewhat RB cord-008590-xivnsldf 224 3 analogous analogous JJ cord-008590-xivnsldf 224 4 situation situation NN cord-008590-xivnsldf 224 5 is be VBZ cord-008590-xivnsldf 224 6 also also RB cord-008590-xivnsldf 224 7 observed observe VBN cord-008590-xivnsldf 224 8 for for IN cord-008590-xivnsldf 224 9 a a DT cord-008590-xivnsldf 224 10 CHO CHO NNP cord-008590-xivnsldf 224 11 cell cell NN cord-008590-xivnsldf 224 12 mutant mutant JJ cord-008590-xivnsldf 224 13 in in IN cord-008590-xivnsldf 224 14 which which WDT cord-008590-xivnsldf 224 15 lysosomal lysosomal JJ cord-008590-xivnsldf 224 16 enzymes enzyme NNS cord-008590-xivnsldf 224 17 bear bear VBP cord-008590-xivnsldf 224 18 only only RB cord-008590-xivnsldf 224 19 complex complex JJ cord-008590-xivnsldf 224 20 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 224 21 ( ( -LRB- cord-008590-xivnsldf 224 22 7 7 CD cord-008590-xivnsldf 224 23 1 1 CD cord-008590-xivnsldf 224 24 a a CD cord-008590-xivnsldf 224 25 ) ) -RRB- cord-008590-xivnsldf 224 26 . . . cord-008590-xivnsldf 225 1 Also also RB cord-008590-xivnsldf 225 2 consistent consistent JJ cord-008590-xivnsldf 225 3 with with IN cord-008590-xivnsldf 225 4 this this DT cord-008590-xivnsldf 225 5 line line NN cord-008590-xivnsldf 225 6 of of IN cord-008590-xivnsldf 225 7 reasoning reasoning NN cord-008590-xivnsldf 225 8 is be VBZ cord-008590-xivnsldf 225 9 a a DT cord-008590-xivnsldf 225 10 report report NN cord-008590-xivnsldf 225 11 that that IN cord-008590-xivnsldf 225 12 the the DT cord-008590-xivnsldf 225 13 mannose mannose NN cord-008590-xivnsldf 225 14 - - HYPH cord-008590-xivnsldf 225 15 phosphate phosphate NN cord-008590-xivnsldf 225 16 receptor receptor NN cord-008590-xivnsldf 225 17 - - : cord-008590-xivnsldf 225 18 which which WDT cord-008590-xivnsldf 225 19 is be VBZ cord-008590-xivnsldf 225 20 thought think VBN cord-008590-xivnsldf 225 21 to to TO cord-008590-xivnsldf 225 22 mediate mediate VB cord-008590-xivnsldf 225 23 the the DT cord-008590-xivnsldf 225 24 ICT ICT NNP cord-008590-xivnsldf 225 25 of of IN cord-008590-xivnsldf 225 26 newly newly RB cord-008590-xivnsldf 225 27 synthesized synthesize VBN cord-008590-xivnsldf 225 28 lysosomal lysosomal JJ cord-008590-xivnsldf 225 29 enzymes enzyme NNS cord-008590-xivnsldf 225 30 - - HYPH cord-008590-xivnsldf 225 31 bears bear NNS cord-008590-xivnsldf 225 32 sialic sialic JJ cord-008590-xivnsldf 225 33 acid acid NN cord-008590-xivnsldf 225 34 ; ; : cord-008590-xivnsldf 225 35 however however RB cord-008590-xivnsldf 225 36 , , , cord-008590-xivnsldf 225 37 the the DT cord-008590-xivnsldf 225 38 receptor receptor NN cord-008590-xivnsldf 225 39 molecules molecule NNS cord-008590-xivnsldf 225 40 isolated isolate VBN cord-008590-xivnsldf 225 41 may may MD cord-008590-xivnsldf 225 42 largely largely RB cord-008590-xivnsldf 225 43 originate originate VB cord-008590-xivnsldf 225 44 from from IN cord-008590-xivnsldf 225 45 the the DT cord-008590-xivnsldf 225 46 cell cell NN cord-008590-xivnsldf 225 47 surface surface NN cord-008590-xivnsldf 225 48 rather rather RB cord-008590-xivnsldf 225 49 than than IN cord-008590-xivnsldf 225 50 from from IN cord-008590-xivnsldf 225 51 the the DT cord-008590-xivnsldf 225 52 GC GC NNP cord-008590-xivnsldf 225 53 ( ( -LRB- cord-008590-xivnsldf 225 54 129 129 CD cord-008590-xivnsldf 225 55 ) ) -RRB- cord-008590-xivnsldf 225 56 . . . cord-008590-xivnsldf 226 1 How how WRB cord-008590-xivnsldf 226 2 does do VBZ cord-008590-xivnsldf 226 3 the the DT cord-008590-xivnsldf 226 4 cell cell NN cord-008590-xivnsldf 226 5 determine determine VB cord-008590-xivnsldf 226 6 whether whether IN cord-008590-xivnsldf 226 7 or or CC cord-008590-xivnsldf 226 8 not not RB cord-008590-xivnsldf 226 9 to to TO cord-008590-xivnsldf 226 10 add add VB cord-008590-xivnsldf 226 11 the the DT cord-008590-xivnsldf 226 12 blocked block VBN cord-008590-xivnsldf 226 13 phosphate phosphate NN cord-008590-xivnsldf 226 14 to to IN cord-008590-xivnsldf 226 15 a a DT cord-008590-xivnsldf 226 16 given give VBN cord-008590-xivnsldf 226 17 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 226 18 ? ? . cord-008590-xivnsldf 227 1 Two two CD cord-008590-xivnsldf 227 2 points point NNS cord-008590-xivnsldf 227 3 should should MD cord-008590-xivnsldf 227 4 be be VB cord-008590-xivnsldf 227 5 made make VBN cord-008590-xivnsldf 227 6 . . . cord-008590-xivnsldf 228 1 ( ( -LRB- cord-008590-xivnsldf 228 2 1 1 CD cord-008590-xivnsldf 228 3 ) ) -RRB- cord-008590-xivnsldf 229 1 The the DT cord-008590-xivnsldf 229 2 signals signal NNS cord-008590-xivnsldf 229 3 have have VBP cord-008590-xivnsldf 229 4 not not RB cord-008590-xivnsldf 229 5 been be VBN cord-008590-xivnsldf 229 6 identified identify VBN cord-008590-xivnsldf 229 7 , , , cord-008590-xivnsldf 229 8 but but CC cord-008590-xivnsldf 229 9 when when WRB cord-008590-xivnsldf 229 10 the the DT cord-008590-xivnsldf 229 11 GlcNAc GlcNAc NNP cord-008590-xivnsldf 229 12 - - HYPH cord-008590-xivnsldf 229 13 phosphotransferase phosphotransferase NNP cord-008590-xivnsldf 229 14 is be VBZ cord-008590-xivnsldf 229 15 presented present VBN cord-008590-xivnsldf 229 16 with with IN cord-008590-xivnsldf 229 17 any any DT cord-008590-xivnsldf 229 18 of of IN cord-008590-xivnsldf 229 19 several several JJ cord-008590-xivnsldf 229 20 high high JJ cord-008590-xivnsldf 229 21 mannose mannose NN cord-008590-xivnsldf 229 22 secretory secretory NN cord-008590-xivnsldf 229 23 proteins protein NNS cord-008590-xivnsldf 229 24 or or CC cord-008590-xivnsldf 229 25 lysosomal lysosomal JJ cord-008590-xivnsldf 229 26 enzymes enzyme NNS cord-008590-xivnsldf 229 27 , , , cord-008590-xivnsldf 229 28 transfer transfer NN cord-008590-xivnsldf 229 29 is be VBZ cord-008590-xivnsldf 229 30 overwhelmingly overwhelmingly RB cord-008590-xivnsldf 229 31 more more RBR cord-008590-xivnsldf 229 32 active active JJ cord-008590-xivnsldf 229 33 toward toward IN cord-008590-xivnsldf 229 34 the the DT cord-008590-xivnsldf 229 35 lysosomal lysosomal JJ cord-008590-xivnsldf 229 36 enzymes enzyme NNS cord-008590-xivnsldf 229 37 ( ( -LRB- cord-008590-xivnsldf 229 38 106 106 CD cord-008590-xivnsldf 229 39 , , , cord-008590-xivnsldf 229 40 144 144 CD cord-008590-xivnsldf 229 41 ) ) -RRB- cord-008590-xivnsldf 229 42 , , , cord-008590-xivnsldf 229 43 and and CC cord-008590-xivnsldf 229 44 ( ( -LRB- cord-008590-xivnsldf 229 45 2 2 LS cord-008590-xivnsldf 229 46 ) ) -RRB- cord-008590-xivnsldf 229 47 the the DT cord-008590-xivnsldf 229 48 studies study NNS cord-008590-xivnsldf 229 49 on on IN cord-008590-xivnsldf 229 50 processing processing NN cord-008590-xivnsldf 229 51 of of IN cord-008590-xivnsldf 229 52 lysosomal lysosomal JJ cord-008590-xivnsldf 229 53 enzymes enzyme NNS cord-008590-xivnsldf 229 54 once once RB cord-008590-xivnsldf 229 55 again again RB cord-008590-xivnsldf 229 56 indicate indicate VBP cord-008590-xivnsldf 229 57 the the DT cord-008590-xivnsldf 229 58 existence existence NN cord-008590-xivnsldf 229 59 of of IN cord-008590-xivnsldf 229 60 microheterogeneity microheterogeneity NN cord-008590-xivnsldf 229 61 : : : cord-008590-xivnsldf 229 62 the the DT cord-008590-xivnsldf 229 63 degree degree NN cord-008590-xivnsldf 229 64 of of IN cord-008590-xivnsldf 229 65 addition addition NN cord-008590-xivnsldf 229 66 of of IN cord-008590-xivnsldf 229 67 blocked block VBN cord-008590-xivnsldf 229 68 phosphate phosphate NN cord-008590-xivnsldf 229 69 , , , cord-008590-xivnsldf 229 70 of of IN cord-008590-xivnsldf 229 71 unblocking unblocking NN cord-008590-xivnsldf 229 72 , , , cord-008590-xivnsldf 229 73 and and CC cord-008590-xivnsldf 229 74 of of IN cord-008590-xivnsldf 229 75 addition addition NN cord-008590-xivnsldf 229 76 of of IN cord-008590-xivnsldf 229 77 terminal terminal JJ cord-008590-xivnsldf 229 78 sugars sugar NNS cord-008590-xivnsldf 229 79 is be VBZ cord-008590-xivnsldf 229 80 variable variable JJ cord-008590-xivnsldf 229 81 . . . cord-008590-xivnsldf 230 1 In in IN cord-008590-xivnsldf 230 2 yeast yeast NN cord-008590-xivnsldf 230 3 , , , cord-008590-xivnsldf 230 4 where where WRB cord-008590-xivnsldf 230 5 lysosomal lysosomal JJ cord-008590-xivnsldf 230 6 enzyme enzyme NN cord-008590-xivnsldf 230 7 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 230 8 are be VBP cord-008590-xivnsldf 230 9 not not RB cord-008590-xivnsldf 230 10 essential essential JJ cord-008590-xivnsldf 230 11 for for IN cord-008590-xivnsldf 230 12 targeting target VBG cord-008590-xivnsldf 230 13 to to IN cord-008590-xivnsldf 230 14 the the DT cord-008590-xivnsldf 230 15 lysosome lysosome NN cord-008590-xivnsldf 230 16 ( ( -LRB- cord-008590-xivnsldf 230 17 1201 1201 CD cord-008590-xivnsldf 230 18 , , , cord-008590-xivnsldf 230 19 lysosomal lysosomal JJ cord-008590-xivnsldf 230 20 carboxypeptidase carboxypeptidase NN cord-008590-xivnsldf 230 21 Y Y NNP cord-008590-xivnsldf 230 22 is be VBZ cord-008590-xivnsldf 230 23 phosphorylated phosphorylate VBN cord-008590-xivnsldf 230 24 while while IN cord-008590-xivnsldf 230 25 in in IN cord-008590-xivnsldf 230 26 the the DT cord-008590-xivnsldf 230 27 RER rer NN cord-008590-xivnsldf 230 28 ( ( -LRB- cord-008590-xivnsldf 230 29 131 131 CD cord-008590-xivnsldf 230 30 ) ) -RRB- cord-008590-xivnsldf 230 31 . . . cord-008590-xivnsldf 231 1 Its -PRON- PRP$ cord-008590-xivnsldf 231 2 carbohydrate carbohydrate NN cord-008590-xivnsldf 231 3 is be VBZ cord-008590-xivnsldf 231 4 processed process VBN cord-008590-xivnsldf 231 5 in in IN cord-008590-xivnsldf 231 6 two two CD cord-008590-xivnsldf 231 7 stages stage NNS cord-008590-xivnsldf 231 8 while while IN cord-008590-xivnsldf 231 9 tranversing tranverse VBG cord-008590-xivnsldf 231 10 the the DT cord-008590-xivnsldf 231 11 GC gc NN cord-008590-xivnsldf 231 12 - - HYPH cord-008590-xivnsldf 231 13 temperature temperature NN cord-008590-xivnsldf 231 14 - - HYPH cord-008590-xivnsldf 231 15 sensitive sensitive JJ cord-008590-xivnsldf 231 16 ICT ICT NNP cord-008590-xivnsldf 231 17 mutants mutant NNS cord-008590-xivnsldf 231 18 blocked block VBN cord-008590-xivnsldf 231 19 at at IN cord-008590-xivnsldf 231 20 the the DT cord-008590-xivnsldf 231 21 level level NN cord-008590-xivnsldf 231 22 of of IN cord-008590-xivnsldf 231 23 the the DT cord-008590-xivnsldf 231 24 GC GC NNP cord-008590-xivnsldf 231 25 accumulate accumulate VB cord-008590-xivnsldf 231 26 one one CD cord-008590-xivnsldf 231 27 of of IN cord-008590-xivnsldf 231 28 two two CD cord-008590-xivnsldf 231 29 endoglucosamindiase endoglucosamindiase NN cord-008590-xivnsldf 231 30 H h NN cord-008590-xivnsldf 231 31 - - HYPH cord-008590-xivnsldf 231 32 sensitive sensitive JJ cord-008590-xivnsldf 231 33 species specie NNS cord-008590-xivnsldf 231 34 ( ( -LRB- cord-008590-xivnsldf 231 35 1 1 CD cord-008590-xivnsldf 231 36 3 3 CD cord-008590-xivnsldf 231 37 1 1 CD cord-008590-xivnsldf 231 38 ) ) -RRB- cord-008590-xivnsldf 231 39 . . . cord-008590-xivnsldf 232 1 Data datum NNS cord-008590-xivnsldf 232 2 on on IN cord-008590-xivnsldf 232 3 the the DT cord-008590-xivnsldf 232 4 influence influence NN cord-008590-xivnsldf 232 5 of of IN cord-008590-xivnsldf 232 6 monensin monensin NNP cord-008590-xivnsldf 232 7 on on IN cord-008590-xivnsldf 232 8 lysosomal lysosomal JJ cord-008590-xivnsldf 232 9 enzyme enzyme NN cord-008590-xivnsldf 232 10 transport transport NN cord-008590-xivnsldf 232 11 are be VBP cord-008590-xivnsldf 232 12 suggestive suggestive JJ cord-008590-xivnsldf 232 13 . . . cord-008590-xivnsldf 233 1 In in IN cord-008590-xivnsldf 233 2 I i NN cord-008590-xivnsldf 233 3 - - HYPH cord-008590-xivnsldf 233 4 cell cell NN cord-008590-xivnsldf 233 5 fibroblasts fibroblast NNS cord-008590-xivnsldf 233 6 monensin monensin NNP cord-008590-xivnsldf 233 7 reduces reduce VBZ cord-008590-xivnsldf 233 8 hexosaminidase hexosaminidase NNP cord-008590-xivnsldf 233 9 secretion secretion NN cord-008590-xivnsldf 233 10 ( ( -LRB- cord-008590-xivnsldf 233 11 143b 143b NNP cord-008590-xivnsldf 233 12 ) ) -RRB- cord-008590-xivnsldf 233 13 . . . cord-008590-xivnsldf 234 1 In in IN cord-008590-xivnsldf 234 2 normal normal JJ cord-008590-xivnsldf 234 3 cells cell NNS cord-008590-xivnsldf 234 4 it -PRON- PRP cord-008590-xivnsldf 234 5 may may MD cord-008590-xivnsldf 234 6 actually actually RB cord-008590-xivnsldf 234 7 stimulate stimulate VB cord-008590-xivnsldf 234 8 their -PRON- PRP$ cord-008590-xivnsldf 234 9 release release NN cord-008590-xivnsldf 234 10 ( ( -LRB- cord-008590-xivnsldf 234 11 100a 100a NNP cord-008590-xivnsldf 234 12 ) ) -RRB- cord-008590-xivnsldf 234 13 . . . cord-008590-xivnsldf 235 1 It -PRON- PRP cord-008590-xivnsldf 235 2 is be VBZ cord-008590-xivnsldf 235 3 striking strike VBG cord-008590-xivnsldf 235 4 that that DT cord-008590-xivnsldf 235 5 chloroquine chloroquine NN cord-008590-xivnsldf 235 6 , , , cord-008590-xivnsldf 235 7 which which WDT cord-008590-xivnsldf 235 8 also also RB cord-008590-xivnsldf 235 9 raises raise VBZ cord-008590-xivnsldf 235 10 intralysosomal intralysosomal NNP cord-008590-xivnsldf 235 11 pH pH NNP cord-008590-xivnsldf 235 12 , , , cord-008590-xivnsldf 235 13 also also RB cord-008590-xivnsldf 235 14 causes cause VBZ cord-008590-xivnsldf 235 15 release release NN cord-008590-xivnsldf 235 16 of of IN cord-008590-xivnsldf 235 17 newly newly RB cord-008590-xivnsldf 235 18 synthesized synthesize VBN cord-008590-xivnsldf 235 19 lysosomal lysosomal JJ cord-008590-xivnsldf 235 20 enzymes enzyme NNS cord-008590-xivnsldf 235 21 ( ( -LRB- cord-008590-xivnsldf 235 22 124 124 CD cord-008590-xivnsldf 235 23 ) ) -RRB- cord-008590-xivnsldf 235 24 . . . cord-008590-xivnsldf 236 1 This this DT cord-008590-xivnsldf 236 2 effect effect NN cord-008590-xivnsldf 236 3 can can MD cord-008590-xivnsldf 236 4 be be VB cord-008590-xivnsldf 236 5 attributed attribute VBN cord-008590-xivnsldf 236 6 to to IN cord-008590-xivnsldf 236 7 neutralization neutralization NN cord-008590-xivnsldf 236 8 of of IN cord-008590-xivnsldf 236 9 lysosomal lysosomal JJ cord-008590-xivnsldf 236 10 content content NN cord-008590-xivnsldf 236 11 by by IN cord-008590-xivnsldf 236 12 the the DT cord-008590-xivnsldf 236 13 ionophore ionophore NNP cord-008590-xivnsldf 236 14 and and CC cord-008590-xivnsldf 236 15 a a DT cord-008590-xivnsldf 236 16 consequent consequent NN cord-008590-xivnsldf 236 17 tying tie VBG cord-008590-xivnsldf 236 18 up up IN cord-008590-xivnsldf 236 19 of of IN cord-008590-xivnsldf 236 20 the the DT cord-008590-xivnsldf 236 21 mannose mannose NN cord-008590-xivnsldf 236 22 - - HYPH cord-008590-xivnsldf 236 23 phosphate phosphate NN cord-008590-xivnsldf 236 24 receptor receptor NN cord-008590-xivnsldf 236 25 : : : cord-008590-xivnsldf 236 26 however however RB cord-008590-xivnsldf 236 27 , , , cord-008590-xivnsldf 236 28 the the DT cord-008590-xivnsldf 236 29 situation situation NN cord-008590-xivnsldf 236 30 has have VBZ cord-008590-xivnsldf 236 31 not not RB cord-008590-xivnsldf 236 32 yet yet RB cord-008590-xivnsldf 236 33 been be VBN cord-008590-xivnsldf 236 34 fully fully RB cord-008590-xivnsldf 236 35 analyzed analyze VBN cord-008590-xivnsldf 236 36 . . . cord-008590-xivnsldf 237 1 The the DT cord-008590-xivnsldf 237 2 secreted secrete VBN cord-008590-xivnsldf 237 3 lysosomal lysosomal JJ cord-008590-xivnsldf 237 4 enzymes enzyme NNS cord-008590-xivnsldf 237 5 are be VBP cord-008590-xivnsldf 237 6 phosphorylated phosphorylate VBN cord-008590-xivnsldf 237 7 ( ( -LRB- cord-008590-xivnsldf 237 8 100a 100a CD cord-008590-xivnsldf 237 9 ) ) -RRB- cord-008590-xivnsldf 237 10 . . . cord-008590-xivnsldf 238 1 Proteins protein NNS cord-008590-xivnsldf 238 2 ( ( -LRB- cord-008590-xivnsldf 238 3 117 117 CD cord-008590-xivnsldf 238 4 , , , cord-008590-xivnsldf 238 5 118 118 CD cord-008590-xivnsldf 238 6 ) ) -RRB- cord-008590-xivnsldf 239 1 The the DT cord-008590-xivnsldf 239 2 presence presence NN cord-008590-xivnsldf 239 3 of of IN cord-008590-xivnsldf 239 4 covalently covalently RB cord-008590-xivnsldf 239 5 bound bind VBN cord-008590-xivnsldf 239 6 lipid lipid NN cord-008590-xivnsldf 239 7 is be VBZ cord-008590-xivnsldf 239 8 not not RB cord-008590-xivnsldf 239 9 restricted restrict VBN cord-008590-xivnsldf 239 10 to to IN cord-008590-xivnsldf 239 11 proteins protein NNS cord-008590-xivnsldf 239 12 which which WDT cord-008590-xivnsldf 239 13 traverse traverse VBP cord-008590-xivnsldf 239 14 the the DT cord-008590-xivnsldf 239 15 secretory secretory JJ cord-008590-xivnsldf 239 16 pathway pathway NN cord-008590-xivnsldf 239 17 ; ; : cord-008590-xivnsldf 239 18 however however RB cord-008590-xivnsldf 239 19 , , , cord-008590-xivnsldf 239 20 a a DT cord-008590-xivnsldf 239 21 number number NN cord-008590-xivnsldf 239 22 of of IN cord-008590-xivnsldf 239 23 plasma plasma NN cord-008590-xivnsldf 239 24 membrane membrane NN cord-008590-xivnsldf 239 25 glycoproteins glycoprotein NNS cord-008590-xivnsldf 239 26 have have VBP cord-008590-xivnsldf 239 27 been be VBN cord-008590-xivnsldf 239 28 shown show VBN cord-008590-xivnsldf 239 29 to to TO cord-008590-xivnsldf 239 30 acquire acquire VB cord-008590-xivnsldf 239 31 fatty fatty JJ cord-008590-xivnsldf 239 32 acids acid NNS cord-008590-xivnsldf 239 33 posttranslationally posttranslationally RB cord-008590-xivnsldf 239 34 . . . cord-008590-xivnsldf 240 1 The the DT cord-008590-xivnsldf 240 2 best well RBS cord-008590-xivnsldf 240 3 studied study VBN cord-008590-xivnsldf 240 4 cases case NNS cord-008590-xivnsldf 240 5 are be VBP cord-008590-xivnsldf 240 6 the the DT cord-008590-xivnsldf 240 7 envelope envelope NN cord-008590-xivnsldf 240 8 glycoproteins glycoprotein NNS cord-008590-xivnsldf 240 9 of of IN cord-008590-xivnsldf 240 10 Vesicular Vesicular NNP cord-008590-xivnsldf 240 11 Stomatitis Stomatitis NNP cord-008590-xivnsldf 240 12 virus virus NN cord-008590-xivnsldf 240 13 , , , cord-008590-xivnsldf 240 14 Semliki Semliki NNP cord-008590-xivnsldf 240 15 Forest Forest NNP cord-008590-xivnsldf 240 16 virus virus NN cord-008590-xivnsldf 240 17 , , , cord-008590-xivnsldf 240 18 and and CC cord-008590-xivnsldf 240 19 Sindbis Sindbis NNP cord-008590-xivnsldf 240 20 virus virus NN cord-008590-xivnsldf 240 21 , , , cord-008590-xivnsldf 240 22 the the DT cord-008590-xivnsldf 240 23 proteolipid proteolipid NN cord-008590-xivnsldf 240 24 of of IN cord-008590-xivnsldf 240 25 myelin myelin NNP cord-008590-xivnsldf 240 26 , , , cord-008590-xivnsldf 240 27 the the DT cord-008590-xivnsldf 240 28 transfemn transfemn NNP cord-008590-xivnsldf 240 29 receptor receptor NN cord-008590-xivnsldf 240 30 , , , cord-008590-xivnsldf 240 31 HLA HLA NNP cord-008590-xivnsldf 240 32 antigens antigen NNS cord-008590-xivnsldf 240 33 , , , cord-008590-xivnsldf 240 34 and and CC cord-008590-xivnsldf 240 35 T200 T200 NNP cord-008590-xivnsldf 240 36 , , , cord-008590-xivnsldf 240 37 a a DT cord-008590-xivnsldf 240 38 lymphocyte lymphocyte NN cord-008590-xivnsldf 240 39 surface surface NN cord-008590-xivnsldf 240 40 antigen antigen NN cord-008590-xivnsldf 240 41 . . . cord-008590-xivnsldf 241 1 The the DT cord-008590-xivnsldf 241 2 covalent covalent JJ cord-008590-xivnsldf 241 3 sites site NNS cord-008590-xivnsldf 241 4 of of IN cord-008590-xivnsldf 241 5 fatty fatty JJ cord-008590-xivnsldf 241 6 acid acid NN cord-008590-xivnsldf 241 7 linkage linkage NN cord-008590-xivnsldf 241 8 are be VBP cord-008590-xivnsldf 241 9 suspected suspect VBN cord-008590-xivnsldf 241 10 to to TO cord-008590-xivnsldf 241 11 be be VB cord-008590-xivnsldf 241 12 cysteine cysteine NN cord-008590-xivnsldf 241 13 or or CC cord-008590-xivnsldf 241 14 serine serine NN cord-008590-xivnsldf 241 15 residues residue NNS cord-008590-xivnsldf 241 16 . . . cord-008590-xivnsldf 242 1 The the DT cord-008590-xivnsldf 242 2 corresponding correspond VBG cord-008590-xivnsldf 242 3 enzymes enzyme NNS cord-008590-xivnsldf 242 4 and and CC cord-008590-xivnsldf 242 5 activated activate VBN cord-008590-xivnsldf 242 6 fatty fatty JJ cord-008590-xivnsldf 242 7 acid acid NN cord-008590-xivnsldf 242 8 precursors precursor NNS cord-008590-xivnsldf 242 9 have have VBP cord-008590-xivnsldf 242 10 not not RB cord-008590-xivnsldf 242 11 been be VBN cord-008590-xivnsldf 242 12 identified identify VBN cord-008590-xivnsldf 242 13 . . . cord-008590-xivnsldf 243 1 Four four CD cord-008590-xivnsldf 243 2 lines line NNS cord-008590-xivnsldf 243 3 of of IN cord-008590-xivnsldf 243 4 experimentation experimentation NN cord-008590-xivnsldf 243 5 suggest suggest VBP cord-008590-xivnsldf 243 6 that that IN cord-008590-xivnsldf 243 7 an an DT cord-008590-xivnsldf 243 8 early early JJ cord-008590-xivnsldf 243 9 Golgi Golgi NNP cord-008590-xivnsldf 243 10 subcompartment subcompartment NN cord-008590-xivnsldf 243 11 is be VBZ cord-008590-xivnsldf 243 12 the the DT cord-008590-xivnsldf 243 13 site site NN cord-008590-xivnsldf 243 14 of of IN cord-008590-xivnsldf 243 15 palmitate palmitate NN cord-008590-xivnsldf 243 16 addition addition NN cord-008590-xivnsldf 243 17 in in IN cord-008590-xivnsldf 243 18 virally virally RB cord-008590-xivnsldf 243 19 infected infect VBN cord-008590-xivnsldf 243 20 cells cell NNS cord-008590-xivnsldf 243 21 . . . cord-008590-xivnsldf 244 1 ( ( -LRB- cord-008590-xivnsldf 244 2 1 1 LS cord-008590-xivnsldf 244 3 ) ) -RRB- cord-008590-xivnsldf 244 4 Pulse pulse NN cord-008590-xivnsldf 244 5 - - HYPH cord-008590-xivnsldf 244 6 chase chase NN cord-008590-xivnsldf 244 7 studies study NNS cord-008590-xivnsldf 244 8 employing employ VBG cord-008590-xivnsldf 244 9 labeled label VBN cord-008590-xivnsldf 244 10 palmitic palmitic JJ cord-008590-xivnsldf 244 11 acid acid NN cord-008590-xivnsldf 244 12 show show NN cord-008590-xivnsldf 244 13 that that IN cord-008590-xivnsldf 244 14 the the DT cord-008590-xivnsldf 244 15 half half JJ cord-008590-xivnsldf 244 16 - - HYPH cord-008590-xivnsldf 244 17 time time NN cord-008590-xivnsldf 244 18 required require VBN cord-008590-xivnsldf 244 19 by by IN cord-008590-xivnsldf 244 20 cycloheximide cycloheximide NN cord-008590-xivnsldf 244 21 to to TO cord-008590-xivnsldf 244 22 shut shut VB cord-008590-xivnsldf 244 23 off off RP cord-008590-xivnsldf 244 24 acylation acylation NN cord-008590-xivnsldf 244 25 of of IN cord-008590-xivnsldf 244 26 viral viral JJ cord-008590-xivnsldf 244 27 proteins protein NNS cord-008590-xivnsldf 244 28 is be VBZ cord-008590-xivnsldf 244 29 15 15 CD cord-008590-xivnsldf 244 30 minutes minute NNS cord-008590-xivnsldf 244 31 . . . cord-008590-xivnsldf 245 1 Hence hence RB cord-008590-xivnsldf 245 2 acylation acylation NN cord-008590-xivnsldf 245 3 is be VBZ cord-008590-xivnsldf 245 4 certainly certainly RB cord-008590-xivnsldf 245 5 not not RB cord-008590-xivnsldf 245 6 a a DT cord-008590-xivnsldf 245 7 cotranslational cotranslational JJ cord-008590-xivnsldf 245 8 event event NN cord-008590-xivnsldf 245 9 ( ( -LRB- cord-008590-xivnsldf 245 10 I i NN cord-008590-xivnsldf 245 11 19 19 CD cord-008590-xivnsldf 245 12 ) ) -RRB- cord-008590-xivnsldf 245 13 . . . cord-008590-xivnsldf 246 1 ( ( -LRB- cord-008590-xivnsldf 246 2 2 2 LS cord-008590-xivnsldf 246 3 ) ) -RRB- cord-008590-xivnsldf 247 1 Within within IN cord-008590-xivnsldf 247 2 only only RB cord-008590-xivnsldf 247 3 3 3 CD cord-008590-xivnsldf 247 4 to to TO cord-008590-xivnsldf 247 5 6 6 CD cord-008590-xivnsldf 247 6 minutes minute NNS cord-008590-xivnsldf 247 7 after after IN cord-008590-xivnsldf 247 8 acylation acylation NN cord-008590-xivnsldf 247 9 these these DT cord-008590-xivnsldf 247 10 proteins protein NNS cord-008590-xivnsldf 247 11 become become VBP cord-008590-xivnsldf 247 12 resistent resistent NN cord-008590-xivnsldf 247 13 to to IN cord-008590-xivnsldf 247 14 endo endo FW cord-008590-xivnsldf 247 15 H H NNP cord-008590-xivnsldf 247 16 ( ( -LRB- cord-008590-xivnsldf 247 17 VSV VSV NNP cord-008590-xivnsldf 247 18 ) ) -RRB- cord-008590-xivnsldf 247 19 or or CC cord-008590-xivnsldf 247 20 undergo undergo VB cord-008590-xivnsldf 247 21 proteolytic proteolytic JJ cord-008590-xivnsldf 247 22 cleavage cleavage NN cord-008590-xivnsldf 247 23 ( ( -LRB- cord-008590-xivnsldf 247 24 Sindbis Sindbis NNP cord-008590-xivnsldf 247 25 ) ) -RRB- cord-008590-xivnsldf 247 26 , , , cord-008590-xivnsldf 247 27 whereas whereas IN cord-008590-xivnsldf 247 28 when when WRB cord-008590-xivnsldf 247 29 amino amino NN cord-008590-xivnsldf 247 30 acids acid NNS cord-008590-xivnsldf 247 31 are be VBP cord-008590-xivnsldf 247 32 used use VBN cord-008590-xivnsldf 247 33 to to TO cord-008590-xivnsldf 247 34 label label VB cord-008590-xivnsldf 247 35 the the DT cord-008590-xivnsldf 247 36 same same JJ cord-008590-xivnsldf 247 37 glycoproteins glycoprotein NNS cord-008590-xivnsldf 247 38 , , , cord-008590-xivnsldf 247 39 the the DT cord-008590-xivnsldf 247 40 half half JJ cord-008590-xivnsldf 247 41 - - HYPH cord-008590-xivnsldf 247 42 time time NN cord-008590-xivnsldf 247 43 for for IN cord-008590-xivnsldf 247 44 acquisition acquisition NN cord-008590-xivnsldf 247 45 of of IN cord-008590-xivnsldf 247 46 endo endo NNP cord-008590-xivnsldf 247 47 H H NNP cord-008590-xivnsldf 247 48 resistance resistance NN cord-008590-xivnsldf 247 49 is be VBZ cord-008590-xivnsldf 247 50 20 20 CD cord-008590-xivnsldf 247 51 - - SYM cord-008590-xivnsldf 247 52 30 30 CD cord-008590-xivnsldf 247 53 minutes minute NNS cord-008590-xivnsldf 247 54 ( ( -LRB- cord-008590-xivnsldf 247 55 1 1 CD cord-008590-xivnsldf 247 56 19 19 CD cord-008590-xivnsldf 247 57 ) ) -RRB- cord-008590-xivnsldf 247 58 . . . cord-008590-xivnsldf 248 1 ( ( -LRB- cord-008590-xivnsldf 248 2 3 3 LS cord-008590-xivnsldf 248 3 ) ) -RRB- cord-008590-xivnsldf 248 4 Subcellular subcellular JJ cord-008590-xivnsldf 248 5 fractionation fractionation NN cord-008590-xivnsldf 248 6 of of IN cord-008590-xivnsldf 248 7 VSV VSV NNP cord-008590-xivnsldf 248 8 - - HYPH cord-008590-xivnsldf 248 9 infected infect VBN cord-008590-xivnsldf 248 10 CHO CHO NNP cord-008590-xivnsldf 248 11 cells cell NNS cord-008590-xivnsldf 248 12 pulse pulse NN cord-008590-xivnsldf 248 13 - - HYPH cord-008590-xivnsldf 248 14 labeled label VBN cord-008590-xivnsldf 248 15 with with IN cord-008590-xivnsldf 248 16 [ [ -LRB- cord-008590-xivnsldf 248 17 3H]palmitate 3h]palmitate CD cord-008590-xivnsldf 248 18 shows show NNS cord-008590-xivnsldf 248 19 that that IN cord-008590-xivnsldf 248 20 acylated acylated NNP cord-008590-xivnsldf 248 21 G G NNP cord-008590-xivnsldf 248 22 is be VBZ cord-008590-xivnsldf 248 23 coisopycnic coisopycnic JJ cord-008590-xivnsldf 248 24 with with IN cord-008590-xivnsldf 248 25 mannosidase mannosidase NN cord-008590-xivnsldf 248 26 I i NN cord-008590-xivnsldf 248 27 activity activity NN cord-008590-xivnsldf 248 28 ( ( -LRB- cord-008590-xivnsldf 248 29 20 20 CD cord-008590-xivnsldf 248 30 ) ) -RRB- cord-008590-xivnsldf 248 31 . . . cord-008590-xivnsldf 249 1 In in IN cord-008590-xivnsldf 249 2 a a DT cord-008590-xivnsldf 249 3 closely closely RB cord-008590-xivnsldf 249 4 related related JJ cord-008590-xivnsldf 249 5 system system NN cord-008590-xivnsldf 249 6 , , , cord-008590-xivnsldf 249 7 however however RB cord-008590-xivnsldf 249 8 , , , cord-008590-xivnsldf 249 9 this this DT cord-008590-xivnsldf 249 10 isopycnic isopycnic JJ cord-008590-xivnsldf 249 11 coincidence coincidence NN cord-008590-xivnsldf 249 12 is be VBZ cord-008590-xivnsldf 249 13 eliminated eliminate VBN cord-008590-xivnsldf 249 14 when when WRB cord-008590-xivnsldf 249 15 the the DT cord-008590-xivnsldf 249 16 density density NN cord-008590-xivnsldf 249 17 of of IN cord-008590-xivnsldf 249 18 " " `` cord-008590-xivnsldf 249 19 medial medial JJ cord-008590-xivnsldf 249 20 " " '' cord-008590-xivnsldf 249 21 Golgi Golgi NNP cord-008590-xivnsldf 249 22 elements element NNS cord-008590-xivnsldf 249 23 containing contain VBG cord-008590-xivnsldf 249 24 the the DT cord-008590-xivnsldf 249 25 acylated acylated JJ cord-008590-xivnsldf 249 26 viral viral JJ cord-008590-xivnsldf 249 27 proteins protein NNS cord-008590-xivnsldf 249 28 is be VBZ cord-008590-xivnsldf 249 29 increased increase VBN cord-008590-xivnsldf 249 30 by by IN cord-008590-xivnsldf 249 31 infecting infect VBG cord-008590-xivnsldf 249 32 the the DT cord-008590-xivnsldf 249 33 cells cell NNS cord-008590-xivnsldf 249 34 with with IN cord-008590-xivnsldf 249 35 Semliki Semliki NNP cord-008590-xivnsldf 249 36 Forest Forest NNP cord-008590-xivnsldf 249 37 virus virus NN cord-008590-xivnsldf 249 38 and and CC cord-008590-xivnsldf 249 39 then then RB cord-008590-xivnsldf 249 40 treating treat VBG cord-008590-xivnsldf 249 41 with with IN cord-008590-xivnsldf 249 42 monensin monensin NNP cord-008590-xivnsldf 249 43 ( ( -LRB- cord-008590-xivnsldf 249 44 101 101 CD cord-008590-xivnsldf 249 45 ) ) -RRB- cord-008590-xivnsldf 249 46 . . . cord-008590-xivnsldf 250 1 In in IN cord-008590-xivnsldf 250 2 this this DT cord-008590-xivnsldf 250 3 situation situation NN cord-008590-xivnsldf 250 4 mannosidose mannosidose DT cord-008590-xivnsldf 250 5 I -PRON- PRP cord-008590-xivnsldf 250 6 is be VBZ cord-008590-xivnsldf 250 7 unperturbed unperturbed JJ cord-008590-xivnsldf 250 8 and and CC cord-008590-xivnsldf 250 9 remains remain VBZ cord-008590-xivnsldf 250 10 coisopycnic coisopycnic NNP cord-008590-xivnsldf 250 11 with with IN cord-008590-xivnsldf 250 12 galactosyl galactosyl NNP cord-008590-xivnsldf 250 13 transferase transferase NNP cord-008590-xivnsldf 250 14 . . . cord-008590-xivnsldf 251 1 ( ( -LRB- cord-008590-xivnsldf 251 2 4 4 LS cord-008590-xivnsldf 251 3 ) ) -RRB- cord-008590-xivnsldf 252 1 The the DT cord-008590-xivnsldf 252 2 acylation acylation NN cord-008590-xivnsldf 252 3 of of IN cord-008590-xivnsldf 252 4 these these DT cord-008590-xivnsldf 252 5 glycoproteins glycoprotein NNS cord-008590-xivnsldf 252 6 is be VBZ cord-008590-xivnsldf 252 7 not not RB cord-008590-xivnsldf 252 8 blocked block VBN cord-008590-xivnsldf 252 9 by by IN cord-008590-xivnsldf 252 10 monensin monensin NNP cord-008590-xivnsldf 252 11 ( ( -LRB- cord-008590-xivnsldf 252 12 54 54 CD cord-008590-xivnsldf 252 13 ) ) -RRB- cord-008590-xivnsldf 252 14 . . . cord-008590-xivnsldf 253 1 It -PRON- PRP cord-008590-xivnsldf 253 2 is be VBZ cord-008590-xivnsldf 253 3 curious curious JJ cord-008590-xivnsldf 253 4 that that IN cord-008590-xivnsldf 253 5 in in IN cord-008590-xivnsldf 253 6 yeast yeast NN cord-008590-xivnsldf 253 7 ICT ICT NNP cord-008590-xivnsldf 253 8 mutants mutant NNS cord-008590-xivnsldf 253 9 acylation acylation NN cord-008590-xivnsldf 253 10 has have VBZ cord-008590-xivnsldf 253 11 been be VBN cord-008590-xivnsldf 253 12 assigned assign VBN cord-008590-xivnsldf 253 13 to to IN cord-008590-xivnsldf 253 14 the the DT cord-008590-xivnsldf 253 15 RER RER NNP cord-008590-xivnsldf 253 16 ( ( -LRB- cord-008590-xivnsldf 253 17 M. M. NNP cord-008590-xivnsldf 253 18 Schlesinger Schlesinger NNP cord-008590-xivnsldf 253 19 , , , cord-008590-xivnsldf 253 20 personal personal JJ cord-008590-xivnsldf 253 21 communication communication NN cord-008590-xivnsldf 253 22 ) ) -RRB- cord-008590-xivnsldf 253 23 . . . cord-008590-xivnsldf 254 1 Since since IN cord-008590-xivnsldf 254 2 the the DT cord-008590-xivnsldf 254 3 phosphorylation phosphorylation NN cord-008590-xivnsldf 254 4 of of IN cord-008590-xivnsldf 254 5 yeast yeast NN cord-008590-xivnsldf 254 6 lysosomal lysosomal JJ cord-008590-xivnsldf 254 7 enzymes enzyme NNS cord-008590-xivnsldf 254 8 also also RB cord-008590-xivnsldf 254 9 occurs occur VBZ cord-008590-xivnsldf 254 10 in in IN cord-008590-xivnsldf 254 11 such such JJ cord-008590-xivnsldf 254 12 RER rer NN cord-008590-xivnsldf 254 13 - - HYPH cord-008590-xivnsldf 254 14 blocked block VBN cord-008590-xivnsldf 254 15 mutants mutant NNS cord-008590-xivnsldf 254 16 ( ( -LRB- cord-008590-xivnsldf 254 17 131 131 CD cord-008590-xivnsldf 254 18 ) ) -RRB- cord-008590-xivnsldf 254 19 , , , cord-008590-xivnsldf 254 20 but but CC cord-008590-xivnsldf 254 21 has have VBZ cord-008590-xivnsldf 254 22 been be VBN cord-008590-xivnsldf 254 23 localized localize VBN cord-008590-xivnsldf 254 24 to to IN cord-008590-xivnsldf 254 25 preparative preparative JJ cord-008590-xivnsldf 254 26 liver liver NN cord-008590-xivnsldf 254 27 Golgi Golgi NNP cord-008590-xivnsldf 254 28 fractions fraction NNS cord-008590-xivnsldf 254 29 ( ( -LRB- cord-008590-xivnsldf 254 30 71 71 CD cord-008590-xivnsldf 254 31 ) ) -RRB- cord-008590-xivnsldf 254 32 , , , cord-008590-xivnsldf 254 33 one one CD cord-008590-xivnsldf 254 34 wonders wonder VBZ cord-008590-xivnsldf 254 35 whether whether IN cord-008590-xivnsldf 254 36 the the DT cord-008590-xivnsldf 254 37 conventional conventional JJ cord-008590-xivnsldf 254 38 anatomic anatomic JJ cord-008590-xivnsldf 254 39 boundary boundary NN cord-008590-xivnsldf 254 40 between between IN cord-008590-xivnsldf 254 41 RER RER NNP cord-008590-xivnsldf 254 42 and and CC cord-008590-xivnsldf 254 43 GC GC NNP cord-008590-xivnsldf 254 44 corresponds correspond VBZ cord-008590-xivnsldf 254 45 to to IN cord-008590-xivnsldf 254 46 the the DT cord-008590-xivnsldf 254 47 mutant mutant JJ cord-008590-xivnsldf 254 48 phenotypes phenotype NNS cord-008590-xivnsldf 254 49 . . . cord-008590-xivnsldf 255 1 In in IN cord-008590-xivnsldf 255 2 higher high JJR cord-008590-xivnsldf 255 3 organisms organism NNS cord-008590-xivnsldf 255 4 a a DT cord-008590-xivnsldf 255 5 partial partial JJ cord-008590-xivnsldf 255 6 enzymologic enzymologic JJ cord-008590-xivnsldf 255 7 overlap overlap NN cord-008590-xivnsldf 255 8 has have VBZ cord-008590-xivnsldf 255 9 been be VBN cord-008590-xivnsldf 255 10 noticed notice VBN cord-008590-xivnsldf 255 11 between between IN cord-008590-xivnsldf 255 12 the the DT cord-008590-xivnsldf 255 13 RER RER NNP cord-008590-xivnsldf 255 14 membranes membrane NNS cord-008590-xivnsldf 255 15 and and CC cord-008590-xivnsldf 255 16 the the DT cord-008590-xivnsldf 255 17 GC GC NNP cord-008590-xivnsldf 255 18 ( ( -LRB- cord-008590-xivnsldf 255 19 49 49 CD cord-008590-xivnsldf 255 20 , , , cord-008590-xivnsldf 255 21 53 53 CD cord-008590-xivnsldf 255 22 , , , cord-008590-xivnsldf 255 23 11 11 CD cord-008590-xivnsldf 255 24 1 1 CD cord-008590-xivnsldf 255 25 ) ) -RRB- cord-008590-xivnsldf 255 26 and and CC cord-008590-xivnsldf 255 27 electron electron NN cord-008590-xivnsldf 255 28 microscopic microscopic JJ cord-008590-xivnsldf 255 29 lectin lectin NN cord-008590-xivnsldf 255 30 - - HYPH cord-008590-xivnsldf 255 31 binding bind VBG cord-008590-xivnsldf 255 32 studies study NNS cord-008590-xivnsldf 255 33 ( ( -LRB- cord-008590-xivnsldf 255 34 36 36 CD cord-008590-xivnsldf 255 35 , , , cord-008590-xivnsldf 255 36 135 135 CD cord-008590-xivnsldf 255 37 , , , cord-008590-xivnsldf 255 38 138 138 CD cord-008590-xivnsldf 255 39 ) ) -RRB- cord-008590-xivnsldf 255 40 suggest suggest VBP cord-008590-xivnsldf 255 41 that that IN cord-008590-xivnsldf 255 42 it -PRON- PRP cord-008590-xivnsldf 255 43 is be VBZ cord-008590-xivnsldf 255 44 the the DT cord-008590-xivnsldf 255 45 anatomically anatomically RB cord-008590-xivnsldf 255 46 proximal proximal JJ cord-008590-xivnsldf 255 47 Golgi Golgi NNP cord-008590-xivnsldf 255 48 cisternae cisternae NNP cord-008590-xivnsldf 255 49 that that WDT cord-008590-xivnsldf 255 50 resemble resemble VBP cord-008590-xivnsldf 255 51 the the DT cord-008590-xivnsldf 255 52 RER rer NN cord-008590-xivnsldf 255 53 . . . cord-008590-xivnsldf 256 1 Moreover moreover RB cord-008590-xivnsldf 256 2 , , , cord-008590-xivnsldf 256 3 as as IN cord-008590-xivnsldf 256 4 already already RB cord-008590-xivnsldf 256 5 mentioned mention VBN cord-008590-xivnsldf 256 6 , , , cord-008590-xivnsldf 256 7 monensin monensin JJ cord-008590-xivnsldf 256 8 experiments experiment NNS cord-008590-xivnsldf 256 9 have have VBP cord-008590-xivnsldf 256 10 assigned assign VBN cord-008590-xivnsldf 256 11 both both DT cord-008590-xivnsldf 256 12 acylation acylation NN cord-008590-xivnsldf 256 13 and and CC cord-008590-xivnsldf 256 14 lysosomal lysosomal JJ cord-008590-xivnsldf 256 15 enzyme enzyme NN cord-008590-xivnsldf 256 16 phosphorylation phosphorylation NN cord-008590-xivnsldf 256 17 to to IN cord-008590-xivnsldf 256 18 an an DT cord-008590-xivnsldf 256 19 early early JJ cord-008590-xivnsldf 256 20 subcompartment subcompartment NN cord-008590-xivnsldf 256 21 . . . cord-008590-xivnsldf 257 1 Perhaps perhaps RB cord-008590-xivnsldf 257 2 in in IN cord-008590-xivnsldf 257 3 the the DT cord-008590-xivnsldf 257 4 yeast yeast NN cord-008590-xivnsldf 257 5 ICT ICT NNP cord-008590-xivnsldf 257 6 mutants mutant NNS cord-008590-xivnsldf 257 7 in in IN cord-008590-xivnsldf 257 8 question question NN cord-008590-xivnsldf 257 9 the the DT cord-008590-xivnsldf 257 10 accumulated accumulate VBN cord-008590-xivnsldf 257 11 unit unit NN cord-008590-xivnsldf 257 12 includes include VBZ cord-008590-xivnsldf 257 13 what what WP cord-008590-xivnsldf 257 14 is be VBZ cord-008590-xivnsldf 257 15 conventionally conventionally RB cord-008590-xivnsldf 257 16 considered consider VBN cord-008590-xivnsldf 257 17 to to TO cord-008590-xivnsldf 257 18 be be VB cord-008590-xivnsldf 257 19 the the DT cord-008590-xivnsldf 257 20 proximal proximal JJ cord-008590-xivnsldf 257 21 face face NN cord-008590-xivnsldf 257 22 of of IN cord-008590-xivnsldf 257 23 the the DT cord-008590-xivnsldf 257 24 GC GC NNP cord-008590-xivnsldf 257 25 as as RB cord-008590-xivnsldf 257 26 well well RB cord-008590-xivnsldf 257 27 as as IN cord-008590-xivnsldf 257 28 the the DT cord-008590-xivnsldf 257 29 RER RER NNP cord-008590-xivnsldf 257 30 . . . cord-008590-xivnsldf 258 1 An an DT cord-008590-xivnsldf 258 2 additional additional JJ cord-008590-xivnsldf 258 3 modification modification NN cord-008590-xivnsldf 258 4 of of IN cord-008590-xivnsldf 258 5 asparagine asparagine NN cord-008590-xivnsldf 258 6 - - HYPH cord-008590-xivnsldf 258 7 linked link VBN cord-008590-xivnsldf 258 8 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 258 9 occurs occur VBZ cord-008590-xivnsldf 258 10 in in IN cord-008590-xivnsldf 258 11 the the DT cord-008590-xivnsldf 258 12 cornea cornea NN cord-008590-xivnsldf 258 13 where where WRB cord-008590-xivnsldf 258 14 they -PRON- PRP cord-008590-xivnsldf 258 15 may may MD cord-008590-xivnsldf 258 16 be be VB cord-008590-xivnsldf 258 17 processed process VBN cord-008590-xivnsldf 258 18 to to IN cord-008590-xivnsldf 258 19 corneal corneal JJ cord-008590-xivnsldf 258 20 keratan keratan NNP cord-008590-xivnsldf 258 21 sulfate sulfate NNP cord-008590-xivnsldf 258 22 , , , cord-008590-xivnsldf 258 23 a a DT cord-008590-xivnsldf 258 24 rather rather RB cord-008590-xivnsldf 258 25 small small JJ cord-008590-xivnsldf 258 26 ( ( -LRB- cord-008590-xivnsldf 258 27 70 70 CD cord-008590-xivnsldf 258 28 kilodalton kilodalton NNS cord-008590-xivnsldf 258 29 ) ) -RRB- cord-008590-xivnsldf 258 30 proteoglycan proteoglycan NN cord-008590-xivnsldf 258 31 characterized characterize VBN cord-008590-xivnsldf 258 32 by by IN cord-008590-xivnsldf 258 33 a a DT cord-008590-xivnsldf 258 34 repeating repeat VBG cord-008590-xivnsldf 258 35 sulfated sulfate VBN cord-008590-xivnsldf 258 36 ( ( -LRB- cord-008590-xivnsldf 258 37 GlcNAc GlcNAc NNP cord-008590-xivnsldf 258 38 , , , cord-008590-xivnsldf 258 39 Gal Gal NNP cord-008590-xivnsldf 258 40 ) ) -RRB- cord-008590-xivnsldf 258 41 disaccharide disaccharide NN cord-008590-xivnsldf 258 42 . . . cord-008590-xivnsldf 259 1 Since since IN cord-008590-xivnsldf 259 2 keratan keratan NNP cord-008590-xivnsldf 259 3 sulfate sulfate NNP cord-008590-xivnsldf 259 4 I -PRON- PRP cord-008590-xivnsldf 259 5 biosynthesis biosynthesis NN cord-008590-xivnsldf 259 6 can can MD cord-008590-xivnsldf 259 7 be be VB cord-008590-xivnsldf 259 8 blocked block VBN cord-008590-xivnsldf 259 9 by by IN cord-008590-xivnsldf 259 10 tunicamycin tunicamycin NNS cord-008590-xivnsldf 259 11 ( ( -LRB- cord-008590-xivnsldf 259 12 43 43 CD cord-008590-xivnsldf 259 13 ) ) -RRB- cord-008590-xivnsldf 259 14 , , , cord-008590-xivnsldf 259 15 these these DT cord-008590-xivnsldf 259 16 units unit NNS cord-008590-xivnsldf 259 17 appear appear VBP cord-008590-xivnsldf 259 18 to to TO cord-008590-xivnsldf 259 19 be be VB cord-008590-xivnsldf 259 20 the the DT cord-008590-xivnsldf 259 21 result result NN cord-008590-xivnsldf 259 22 of of IN cord-008590-xivnsldf 259 23 an an DT cord-008590-xivnsldf 259 24 alternate alternate JJ cord-008590-xivnsldf 259 25 derivatization derivatization NN cord-008590-xivnsldf 259 26 of of IN cord-008590-xivnsldf 259 27 the the DT cord-008590-xivnsldf 259 28 same same JJ cord-008590-xivnsldf 259 29 trimmed trim VBN cord-008590-xivnsldf 259 30 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 259 31 which which WDT cord-008590-xivnsldf 259 32 can can MD cord-008590-xivnsldf 259 33 be be VB cord-008590-xivnsldf 259 34 processed process VBN cord-008590-xivnsldf 259 35 to to IN cord-008590-xivnsldf 259 36 a a DT cord-008590-xivnsldf 259 37 sialic sialic JJ cord-008590-xivnsldf 259 38 acid acid NN cord-008590-xivnsldf 259 39 - - HYPH cord-008590-xivnsldf 259 40 containing contain VBG cord-008590-xivnsldf 259 41 complex complex JJ cord-008590-xivnsldf 259 42 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 259 43 . . . cord-008590-xivnsldf 260 1 Indeed indeed RB cord-008590-xivnsldf 260 2 , , , cord-008590-xivnsldf 260 3 the the DT cord-008590-xivnsldf 260 4 linkage linkage NN cord-008590-xivnsldf 260 5 region region NN cord-008590-xivnsldf 260 6 is be VBZ cord-008590-xivnsldf 260 7 identical identical JJ cord-008590-xivnsldf 260 8 to to IN cord-008590-xivnsldf 260 9 that that DT cord-008590-xivnsldf 260 10 of of IN cord-008590-xivnsldf 260 11 the the DT cord-008590-xivnsldf 260 12 complex complex JJ cord-008590-xivnsldf 260 13 sites site NNS cord-008590-xivnsldf 260 14 - - : cord-008590-xivnsldf 260 15 only only RB cord-008590-xivnsldf 260 16 three three CD cord-008590-xivnsldf 260 17 mannose mannose NN cord-008590-xivnsldf 260 18 residues residue NNS cord-008590-xivnsldf 260 19 remain remain VBP cord-008590-xivnsldf 260 20 and and CC cord-008590-xivnsldf 260 21 these these DT cord-008590-xivnsldf 260 22 bear bear NN cord-008590-xivnsldf 261 1 GlcNAc GlcNAc NNP cord-008590-xivnsldf 261 2 and and CC cord-008590-xivnsldf 261 3 Gal Gal NNP cord-008590-xivnsldf 261 4 followed follow VBN cord-008590-xivnsldf 261 5 by by IN cord-008590-xivnsldf 261 6 the the DT cord-008590-xivnsldf 261 7 repeating repeat VBG cord-008590-xivnsldf 261 8 disaccharide disaccharide NN cord-008590-xivnsldf 261 9 ( ( -LRB- cord-008590-xivnsldf 261 10 89 89 CD cord-008590-xivnsldf 261 11 ) ) -RRB- cord-008590-xivnsldf 261 12 . . . cord-008590-xivnsldf 262 1 Present present JJ cord-008590-xivnsldf 262 2 data datum NNS cord-008590-xivnsldf 262 3 are be VBP cord-008590-xivnsldf 262 4 largely largely RB cord-008590-xivnsldf 262 5 in in IN cord-008590-xivnsldf 262 6 favor favor NN cord-008590-xivnsldf 262 7 of of IN cord-008590-xivnsldf 262 8 the the DT cord-008590-xivnsldf 262 9 notion notion NN cord-008590-xivnsldf 262 10 that that IN cord-008590-xivnsldf 262 11 the the DT cord-008590-xivnsldf 262 12 addition addition NN cord-008590-xivnsldf 262 13 of of IN cord-008590-xivnsldf 262 14 the the DT cord-008590-xivnsldf 262 15 most most RBS cord-008590-xivnsldf 262 16 internal internal JJ cord-008590-xivnsldf 262 17 GalNAc galnac NN cord-008590-xivnsldf 262 18 to to IN cord-008590-xivnsldf 262 19 serine serine NN cord-008590-xivnsldf 262 20 or or CC cord-008590-xivnsldf 262 21 threonine threonine NN cord-008590-xivnsldf 262 22 takes take VBZ cord-008590-xivnsldf 262 23 place place NN cord-008590-xivnsldf 262 24 in in IN cord-008590-xivnsldf 262 25 the the DT cord-008590-xivnsldf 262 26 GC GC NNP cord-008590-xivnsldf 262 27 , , , cord-008590-xivnsldf 262 28 long long RB cord-008590-xivnsldf 262 29 after after IN cord-008590-xivnsldf 262 30 polypeptide polypeptide NN cord-008590-xivnsldf 262 31 chain chain NN cord-008590-xivnsldf 262 32 termination termination NN cord-008590-xivnsldf 262 33 . . . cord-008590-xivnsldf 263 1 The the DT cord-008590-xivnsldf 263 2 data datum NNS cord-008590-xivnsldf 263 3 derive derive VBP cord-008590-xivnsldf 263 4 from from IN cord-008590-xivnsldf 263 5 the the DT cord-008590-xivnsldf 263 6 distribution distribution NN cord-008590-xivnsldf 263 7 of of IN cord-008590-xivnsldf 263 8 GalNAc galnac NN cord-008590-xivnsldf 263 9 transferase transferase NN cord-008590-xivnsldf 263 10 activity activity NN cord-008590-xivnsldf 263 11 in in IN cord-008590-xivnsldf 263 12 subcellular subcellular JJ cord-008590-xivnsldf 263 13 fractions fraction NNS cord-008590-xivnsldf 263 14 of of IN cord-008590-xivnsldf 263 15 intestinal intestinal JJ cord-008590-xivnsldf 263 16 mucosa mucosa NN cord-008590-xivnsldf 263 17 ( ( -LRB- cord-008590-xivnsldf 263 18 61 61 CD cord-008590-xivnsldf 263 19 ) ) -RRB- cord-008590-xivnsldf 263 20 , , , cord-008590-xivnsldf 263 21 and and CC cord-008590-xivnsldf 263 22 oviduct oviduct NN cord-008590-xivnsldf 263 23 ( ( -LRB- cord-008590-xivnsldf 263 24 42 42 CD cord-008590-xivnsldf 263 25 ) ) -RRB- cord-008590-xivnsldf 263 26 , , , cord-008590-xivnsldf 263 27 from from IN cord-008590-xivnsldf 263 28 study study NN cord-008590-xivnsldf 263 29 of of IN cord-008590-xivnsldf 263 30 the the DT cord-008590-xivnsldf 263 31 impact impact NN cord-008590-xivnsldf 263 32 of of IN cord-008590-xivnsldf 263 33 cycloheximide cycloheximide NN cord-008590-xivnsldf 263 34 on on IN cord-008590-xivnsldf 263 35 GalNAc GalNAc NNP cord-008590-xivnsldf 263 36 addition addition NN cord-008590-xivnsldf 263 37 to to IN cord-008590-xivnsldf 263 38 mammary mammary JJ cord-008590-xivnsldf 263 39 glycoproteins glycoprotein NNS cord-008590-xivnsldf 263 40 ( ( -LRB- cord-008590-xivnsldf 263 41 146 146 CD cord-008590-xivnsldf 263 42 ) ) -RRB- cord-008590-xivnsldf 263 43 , , , cord-008590-xivnsldf 263 44 and and CC cord-008590-xivnsldf 263 45 from from IN cord-008590-xivnsldf 263 46 the the DT cord-008590-xivnsldf 263 47 comparative comparative JJ cord-008590-xivnsldf 263 48 evaluation evaluation NN cord-008590-xivnsldf 263 49 of of IN cord-008590-xivnsldf 263 50 the the DT cord-008590-xivnsldf 263 51 kinetics kinetic NNS cord-008590-xivnsldf 263 52 of of IN cord-008590-xivnsldf 263 53 GalNAc GalNAc NNP cord-008590-xivnsldf 263 54 and and CC cord-008590-xivnsldf 263 55 GlcNAc GlcNAc NNP cord-008590-xivnsldf 263 56 addition addition NN cord-008590-xivnsldf 263 57 to to IN cord-008590-xivnsldf 263 58 O o NN cord-008590-xivnsldf 263 59 - - HYPH cord-008590-xivnsldf 263 60 linked link VBN cord-008590-xivnsldf 263 61 and and CC cord-008590-xivnsldf 263 62 N n CD cord-008590-xivnsldf 263 63 - - HYPH cord-008590-xivnsldf 263 64 linked link VBN cord-008590-xivnsldf 263 65 units unit NNS cord-008590-xivnsldf 263 66 , , , cord-008590-xivnsldf 263 67 respectively respectively RB cord-008590-xivnsldf 263 68 ( ( -LRB- cord-008590-xivnsldf 263 69 41 41 CD cord-008590-xivnsldf 263 70 ) ) -RRB- cord-008590-xivnsldf 263 71 . . . cord-008590-xivnsldf 264 1 The the DT cord-008590-xivnsldf 264 2 result result NN cord-008590-xivnsldf 264 3 of of IN cord-008590-xivnsldf 264 4 glycosylation glycosylation NN cord-008590-xivnsldf 264 5 is be VBZ cord-008590-xivnsldf 264 6 most most RBS cord-008590-xivnsldf 264 7 impressive impressive JJ cord-008590-xivnsldf 264 8 for for IN cord-008590-xivnsldf 264 9 mucins mucin NNS cord-008590-xivnsldf 264 10 - - : cord-008590-xivnsldf 264 11 hundreds hundred NNS cord-008590-xivnsldf 264 12 of of IN cord-008590-xivnsldf 264 13 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 264 14 are be VBP cord-008590-xivnsldf 264 15 added add VBN cord-008590-xivnsldf 264 16 and and CC cord-008590-xivnsldf 264 17 the the DT cord-008590-xivnsldf 264 18 product product NN cord-008590-xivnsldf 264 19 may may MD cord-008590-xivnsldf 264 20 be be VB cord-008590-xivnsldf 264 21 greater great JJR cord-008590-xivnsldf 264 22 than than IN cord-008590-xivnsldf 264 23 80 80 CD cord-008590-xivnsldf 264 24 % % NN cord-008590-xivnsldf 264 25 carbohydrate carbohydrate NN cord-008590-xivnsldf 264 26 ( ( -LRB- cord-008590-xivnsldf 264 27 1 1 CD cord-008590-xivnsldf 264 28 1 1 CD cord-008590-xivnsldf 264 29 ) ) -RRB- cord-008590-xivnsldf 264 30 . . . cord-008590-xivnsldf 265 1 The the DT cord-008590-xivnsldf 265 2 enzymes enzyme NNS cord-008590-xivnsldf 265 3 responsible responsible JJ cord-008590-xivnsldf 265 4 for for IN cord-008590-xivnsldf 265 5 chain chain NN cord-008590-xivnsldf 265 6 elongation elongation NN cord-008590-xivnsldf 265 7 have have VBP cord-008590-xivnsldf 265 8 been be VBN cord-008590-xivnsldf 265 9 studied study VBN cord-008590-xivnsldf 265 10 in in IN cord-008590-xivnsldf 265 11 sufficient sufficient JJ cord-008590-xivnsldf 265 12 detail detail NN cord-008590-xivnsldf 265 13 as as IN cord-008590-xivnsldf 265 14 to to TO cord-008590-xivnsldf 265 15 rationalize rationalize VB cord-008590-xivnsldf 265 16 the the DT cord-008590-xivnsldf 265 17 set set NN cord-008590-xivnsldf 265 18 of of IN cord-008590-xivnsldf 265 19 structures structure NNS cord-008590-xivnsldf 265 20 observed observe VBN cord-008590-xivnsldf 265 21 . . . cord-008590-xivnsldf 266 1 It -PRON- PRP cord-008590-xivnsldf 266 2 has have VBZ cord-008590-xivnsldf 266 3 been be VBN cord-008590-xivnsldf 266 4 reported report VBN cord-008590-xivnsldf 266 5 that that IN cord-008590-xivnsldf 266 6 neuraminic neuraminic JJ cord-008590-xivnsldf 266 7 acid acid NN cord-008590-xivnsldf 266 8 of of IN cord-008590-xivnsldf 266 9 mucins mucin NNS cord-008590-xivnsldf 266 10 can can MD cord-008590-xivnsldf 266 11 be be VB cord-008590-xivnsldf 266 12 acetylated acetylate VBN cord-008590-xivnsldf 266 13 and and CC cord-008590-xivnsldf 266 14 that that IN cord-008590-xivnsldf 266 15 acetyl acetyl NN cord-008590-xivnsldf 266 16 groups group NNS cord-008590-xivnsldf 266 17 can can MD cord-008590-xivnsldf 266 18 be be VB cord-008590-xivnsldf 266 19 replaced replace VBN cord-008590-xivnsldf 266 20 by by IN cord-008590-xivnsldf 266 21 glycolyl glycolyl NNP cord-008590-xivnsldf 266 22 groups group NNS cord-008590-xivnsldf 266 23 after after IN cord-008590-xivnsldf 266 24 their -PRON- PRP$ cord-008590-xivnsldf 266 25 addition addition NN cord-008590-xivnsldf 266 26 to to IN cord-008590-xivnsldf 266 27 mucin mucin NN cord-008590-xivnsldf 266 28 ( ( -LRB- cord-008590-xivnsldf 266 29 1 1 CD cord-008590-xivnsldf 266 30 16 16 CD cord-008590-xivnsldf 266 31 ) ) -RRB- cord-008590-xivnsldf 266 32 . . . cord-008590-xivnsldf 267 1 Two two CD cord-008590-xivnsldf 267 2 approaches approach NNS cord-008590-xivnsldf 267 3 localize localize VBP cord-008590-xivnsldf 267 4 mucin mucin NN cord-008590-xivnsldf 267 5 - - HYPH cord-008590-xivnsldf 267 6 specific specific JJ cord-008590-xivnsldf 267 7 transferase transferase NN cord-008590-xivnsldf 267 8 activities activity NNS cord-008590-xivnsldf 267 9 to to IN cord-008590-xivnsldf 267 10 Golgi Golgi NNP cord-008590-xivnsldf 267 11 subcompartments subcompartment NNS cord-008590-xivnsldf 267 12 : : : cord-008590-xivnsldf 267 13 ( ( -LRB- cord-008590-xivnsldf 267 14 1 1 LS cord-008590-xivnsldf 267 15 ) ) -RRB- cord-008590-xivnsldf 267 16 an an DT cord-008590-xivnsldf 267 17 autoradiographic autoradiographic JJ cord-008590-xivnsldf 267 18 study study NN cord-008590-xivnsldf 267 19 of of IN cord-008590-xivnsldf 267 20 [ [ -LRB- cord-008590-xivnsldf 267 21 3H]Gal 3h]gal CD cord-008590-xivnsldf 267 22 pulse pulse NN cord-008590-xivnsldf 267 23 - - HYPH cord-008590-xivnsldf 267 24 labeled label VBN cord-008590-xivnsldf 267 25 mucus mucus NN cord-008590-xivnsldf 267 26 secreting secrete VBG cord-008590-xivnsldf 267 27 cells cell NNS cord-008590-xivnsldf 267 28 which which WDT cord-008590-xivnsldf 267 29 indicates indicate VBZ cord-008590-xivnsldf 267 30 that that IN cord-008590-xivnsldf 267 31 incorporation incorporation NN cord-008590-xivnsldf 267 32 takes take VBZ cord-008590-xivnsldf 267 33 place place NN cord-008590-xivnsldf 267 34 over over IN cord-008590-xivnsldf 267 35 distal distal JJ cord-008590-xivnsldf 267 36 cisternae cisternae NN cord-008590-xivnsldf 267 37 and and CC cord-008590-xivnsldf 267 38 associated associated JJ cord-008590-xivnsldf 267 39 vesicles vesicle NNS cord-008590-xivnsldf 267 40 ( ( -LRB- cord-008590-xivnsldf 267 41 72 72 CD cord-008590-xivnsldf 267 42 ) ) -RRB- cord-008590-xivnsldf 267 43 , , , cord-008590-xivnsldf 267 44 and and CC cord-008590-xivnsldf 267 45 ( ( -LRB- cord-008590-xivnsldf 267 46 2 2 LS cord-008590-xivnsldf 267 47 ) ) -RRB- cord-008590-xivnsldf 267 48 a a DT cord-008590-xivnsldf 267 49 cytochemical cytochemical JJ cord-008590-xivnsldf 267 50 study study NN cord-008590-xivnsldf 267 51 of of IN cord-008590-xivnsldf 267 52 thin thin JJ cord-008590-xivnsldf 267 53 sections section NNS cord-008590-xivnsldf 267 54 of of IN cord-008590-xivnsldf 267 55 goblet goblet NN cord-008590-xivnsldf 267 56 cells cell NNS cord-008590-xivnsldf 267 57 stained stain VBN cord-008590-xivnsldf 267 58 with with IN cord-008590-xivnsldf 267 59 lectin lectin NN cord-008590-xivnsldf 267 60 - - HYPH cord-008590-xivnsldf 267 61 gold gold NN cord-008590-xivnsldf 267 62 conjugates conjugate NNS cord-008590-xivnsldf 267 63 to to TO cord-008590-xivnsldf 267 64 detect detect VB cord-008590-xivnsldf 267 65 nonreducing nonreducing NN cord-008590-xivnsldf 267 66 terminal terminal NN cord-008590-xivnsldf 267 67 GalNAc GalNAc NNP cord-008590-xivnsldf 267 68 ( ( -LRB- cord-008590-xivnsldf 267 69 109 109 CD cord-008590-xivnsldf 267 70 ) ) -RRB- cord-008590-xivnsldf 267 71 . . . cord-008590-xivnsldf 268 1 Proximal Proximal NNP cord-008590-xivnsldf 268 2 cisternae cisternae NNP cord-008590-xivnsldf 268 3 are be VBP cord-008590-xivnsldf 268 4 stained stain VBN cord-008590-xivnsldf 268 5 and and CC cord-008590-xivnsldf 268 6 medial medial JJ cord-008590-xivnsldf 268 7 cisternae cisternae JJ cord-008590-xivnsldf 268 8 negative negative NN cord-008590-xivnsldf 268 9 . . . cord-008590-xivnsldf 269 1 Only only RB cord-008590-xivnsldf 269 2 in in IN cord-008590-xivnsldf 269 3 blood blood NN cord-008590-xivnsldf 269 4 group group NN cord-008590-xivnsldf 269 5 A a NN cord-008590-xivnsldf 269 6 + + FW cord-008590-xivnsldf 269 7 material material NN cord-008590-xivnsldf 269 8 ( ( -LRB- cord-008590-xivnsldf 269 9 where where WRB cord-008590-xivnsldf 269 10 terminal terminal JJ cord-008590-xivnsldf 269 11 GalNAc GalNAc NNP cord-008590-xivnsldf 269 12 is be VBZ cord-008590-xivnsldf 269 13 present present JJ cord-008590-xivnsldf 269 14 ) ) -RRB- cord-008590-xivnsldf 269 15 arc arc NN cord-008590-xivnsldf 269 16 : : : cord-008590-xivnsldf 270 1 the the DT cord-008590-xivnsldf 270 2 distal distal JJ cord-008590-xivnsldf 270 3 cisternae cisternae NN cord-008590-xivnsldf 270 4 and and CC cord-008590-xivnsldf 270 5 secretion secretion NN cord-008590-xivnsldf 270 6 granules granule NNS cord-008590-xivnsldf 270 7 also also RB cord-008590-xivnsldf 270 8 stained stain VBN cord-008590-xivnsldf 270 9 . . . cord-008590-xivnsldf 271 1 Both both DT cord-008590-xivnsldf 271 2 studies study NNS cord-008590-xivnsldf 271 3 are be VBP cord-008590-xivnsldf 271 4 , , , cord-008590-xivnsldf 271 5 therefore therefore RB cord-008590-xivnsldf 271 6 , , , cord-008590-xivnsldf 271 7 consistent consistent JJ cord-008590-xivnsldf 271 8 with with IN cord-008590-xivnsldf 271 9 the the DT cord-008590-xivnsldf 271 10 idea idea NN cord-008590-xivnsldf 271 11 of of IN cord-008590-xivnsldf 271 12 progressive progressive JJ cord-008590-xivnsldf 271 13 sugar sugar NN cord-008590-xivnsldf 271 14 addition addition NN cord-008590-xivnsldf 271 15 during during IN cord-008590-xivnsldf 271 16 proximal proximal JJ cord-008590-xivnsldf 271 17 - - HYPH cord-008590-xivnsldf 271 18 to to IN cord-008590-xivnsldf 271 19 - - HYPH cord-008590-xivnsldf 271 20 distal distal JJ cord-008590-xivnsldf 271 21 transport transport NN cord-008590-xivnsldf 271 22 across across IN cord-008590-xivnsldf 271 23 the the DT cord-008590-xivnsldf 271 24 Golgi Golgi NNP cord-008590-xivnsldf 271 25 stack stack NN cord-008590-xivnsldf 271 26 . . . cord-008590-xivnsldf 272 1 A a DT cord-008590-xivnsldf 272 2 single single JJ cord-008590-xivnsldf 272 3 investigation investigation NN cord-008590-xivnsldf 272 4 indicates indicate VBZ cord-008590-xivnsldf 272 5 that that IN cord-008590-xivnsldf 272 6 the the DT cord-008590-xivnsldf 272 7 subcellular subcellular JJ cord-008590-xivnsldf 272 8 site site NN cord-008590-xivnsldf 272 9 of of IN cord-008590-xivnsldf 272 10 incorporation incorporation NN cord-008590-xivnsldf 272 11 of of IN cord-008590-xivnsldf 272 12 the the DT cord-008590-xivnsldf 272 13 The the DT cord-008590-xivnsldf 272 14 best well RBS cord-008590-xivnsldf 272 15 characterized characterize VBN cord-008590-xivnsldf 272 16 surface surface NN cord-008590-xivnsldf 272 17 glycoprotein glycoprotein NN cord-008590-xivnsldf 272 18 of of IN cord-008590-xivnsldf 272 19 the the DT cord-008590-xivnsldf 272 20 human human JJ cord-008590-xivnsldf 272 21 red red JJ cord-008590-xivnsldf 272 22 blood blood NN cord-008590-xivnsldf 272 23 cell cell NN cord-008590-xivnsldf 272 24 , , , cord-008590-xivnsldf 272 25 glycophorin glycophorin NNP cord-008590-xivnsldf 272 26 , , , cord-008590-xivnsldf 272 27 bears bear VBZ cord-008590-xivnsldf 272 28 15 15 CD cord-008590-xivnsldf 272 29 0-linked 0-linked CD cord-008590-xivnsldf 272 30 tetrasaccharides tetrasaccharide NNS cord-008590-xivnsldf 272 31 and and CC cord-008590-xivnsldf 272 32 one one CD cord-008590-xivnsldf 272 33 N n CD cord-008590-xivnsldf 272 34 - - HYPH cord-008590-xivnsldf 272 35 linked link VBN cord-008590-xivnsldf 272 36 oligosac oligosac NN cord-008590-xivnsldf 272 37 - - HYPH cord-008590-xivnsldf 272 38 charide charide NN cord-008590-xivnsldf 272 39 . . . cord-008590-xivnsldf 273 1 The the DT cord-008590-xivnsldf 273 2 O o NN cord-008590-xivnsldf 273 3 - - HYPH cord-008590-xivnsldf 273 4 linked link VBN cord-008590-xivnsldf 273 5 units unit NNS cord-008590-xivnsldf 273 6 , , , cord-008590-xivnsldf 273 7 which which WDT cord-008590-xivnsldf 273 8 begin begin VBP cord-008590-xivnsldf 273 9 with with IN cord-008590-xivnsldf 273 10 GalNAc GalNAc NNP cord-008590-xivnsldf 273 11 , , , cord-008590-xivnsldf 273 12 should should MD cord-008590-xivnsldf 273 13 serve serve VB cord-008590-xivnsldf 273 14 as as IN cord-008590-xivnsldf 273 15 a a DT cord-008590-xivnsldf 273 16 model model NN cord-008590-xivnsldf 273 17 for for IN cord-008590-xivnsldf 273 18 step step NN cord-008590-xivnsldf 273 19 - - HYPH cord-008590-xivnsldf 273 20 by by IN cord-008590-xivnsldf 273 21 - - HYPH cord-008590-xivnsldf 273 22 step step NN cord-008590-xivnsldf 273 23 analysis analysis NN cord-008590-xivnsldf 273 24 of of IN cord-008590-xivnsldf 273 25 chain chain NN cord-008590-xivnsldf 273 26 elongation elongation NN cord-008590-xivnsldf 273 27 , , , cord-008590-xivnsldf 273 28 as as IN cord-008590-xivnsldf 273 29 should should MD cord-008590-xivnsldf 273 30 the the DT cord-008590-xivnsldf 273 31 O o NN cord-008590-xivnsldf 273 32 - - HYPH cord-008590-xivnsldf 273 33 linked link VBN cord-008590-xivnsldf 273 34 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 273 35 of of IN cord-008590-xivnsldf 273 36 the the DT cord-008590-xivnsldf 273 37 envelope envelope NN cord-008590-xivnsldf 273 38 glycoproteins glycoprotein NNS cord-008590-xivnsldf 273 39 of of IN cord-008590-xivnsldf 273 40 murine murine JJ cord-008590-xivnsldf 273 41 corona corona NN cord-008590-xivnsldf 273 42 virus virus NN cord-008590-xivnsldf 273 43 . . . cord-008590-xivnsldf 274 1 The the DT cord-008590-xivnsldf 274 2 incomplete incomplete JJ cord-008590-xivnsldf 274 3 biosynthetic biosynthetic JJ cord-008590-xivnsldf 274 4 data datum NNS cord-008590-xivnsldf 274 5 suggest suggest VBP cord-008590-xivnsldf 274 6 , , , cord-008590-xivnsldf 274 7 paradoxically paradoxically RB cord-008590-xivnsldf 274 8 , , , cord-008590-xivnsldf 274 9 that that IN cord-008590-xivnsldf 274 10 O o NN cord-008590-xivnsldf 274 11 - - HYPH cord-008590-xivnsldf 274 12 linked link VBN cord-008590-xivnsldf 274 13 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 274 14 are be VBP cord-008590-xivnsldf 274 15 added add VBN cord-008590-xivnsldf 274 16 when when WRB cord-008590-xivnsldf 274 17 the the DT cord-008590-xivnsldf 274 18 glycophorin glycophorin NNP cord-008590-xivnsldf 274 19 message message NN cord-008590-xivnsldf 274 20 is be VBZ cord-008590-xivnsldf 274 21 translated translate VBN cord-008590-xivnsldf 274 22 in in IN cord-008590-xivnsldf 274 23 the the DT cord-008590-xivnsldf 274 24 presence presence NN cord-008590-xivnsldf 274 25 of of IN cord-008590-xivnsldf 274 26 dog dog NNP cord-008590-xivnsldf 274 27 pancrease pancrease NNP cord-008590-xivnsldf 274 28 microsomes microsomes NNP cord-008590-xivnsldf 274 29 ( ( -LRB- cord-008590-xivnsldf 274 30 55 55 CD cord-008590-xivnsldf 274 31 ) ) -RRB- cord-008590-xivnsldf 274 32 . . . cord-008590-xivnsldf 275 1 The the DT cord-008590-xivnsldf 275 2 only only JJ cord-008590-xivnsldf 275 3 published publish VBN cord-008590-xivnsldf 275 4 data datum NNS cord-008590-xivnsldf 275 5 on on IN cord-008590-xivnsldf 275 6 the the DT cord-008590-xivnsldf 275 7 corona corona NN cord-008590-xivnsldf 275 8 virus virus NN cord-008590-xivnsldf 275 9 show show VBP cord-008590-xivnsldf 275 10 that that IN cord-008590-xivnsldf 275 11 very very RB cord-008590-xivnsldf 275 12 long long JJ cord-008590-xivnsldf 275 13 - - HYPH cord-008590-xivnsldf 275 14 term term NN cord-008590-xivnsldf 275 15 monensin monensin JJ cord-008590-xivnsldf 275 16 treatment treatment NN cord-008590-xivnsldf 275 17 blocks block VBZ cord-008590-xivnsldf 275 18 the the DT cord-008590-xivnsldf 275 19 addition addition NN cord-008590-xivnsldf 275 20 of of IN cord-008590-xivnsldf 275 21 O o NN cord-008590-xivnsldf 275 22 - - HYPH cord-008590-xivnsldf 275 23 linked link VBN cord-008590-xivnsldf 275 24 sugars sugar NNS cord-008590-xivnsldf 275 25 ( ( -LRB- cord-008590-xivnsldf 275 26 88 88 CD cord-008590-xivnsldf 275 27 ) ) -RRB- cord-008590-xivnsldf 275 28 . . . cord-008590-xivnsldf 276 1 A a DT cord-008590-xivnsldf 276 2 similar similar JJ cord-008590-xivnsldf 276 3 observation observation NN cord-008590-xivnsldf 276 4 has have VBZ cord-008590-xivnsldf 276 5 been be VBN cord-008590-xivnsldf 276 6 made make VBN cord-008590-xivnsldf 276 7 in in IN cord-008590-xivnsldf 276 8 the the DT cord-008590-xivnsldf 276 9 study study NN cord-008590-xivnsldf 276 10 of of IN cord-008590-xivnsldf 276 11 the the DT cord-008590-xivnsldf 276 12 influence influence NN cord-008590-xivnsldf 276 13 of of IN cord-008590-xivnsldf 276 14 monensin monensin NNP cord-008590-xivnsldf 276 15 on on IN cord-008590-xivnsldf 276 16 the the DT cord-008590-xivnsldf 276 17 biosynthesis biosynthesis NN cord-008590-xivnsldf 276 18 of of IN cord-008590-xivnsldf 276 19 the the DT cord-008590-xivnsldf 276 20 O o NN cord-008590-xivnsldf 276 21 - - HYPH cord-008590-xivnsldf 276 22 linked link VBN cord-008590-xivnsldf 276 23 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 276 24 of of IN cord-008590-xivnsldf 276 25 Herpes Herpes NNP cord-008590-xivnsldf 276 26 virus virus NN cord-008590-xivnsldf 276 27 glycoproteins glycoprotein NNS cord-008590-xivnsldf 276 28 ( ( -LRB- cord-008590-xivnsldf 276 29 54a 54a NNS cord-008590-xivnsldf 276 30 ) ) -RRB- cord-008590-xivnsldf 276 31 . . . cord-008590-xivnsldf 277 1 Skeletal skeletal JJ cord-008590-xivnsldf 277 2 keratan keratan NNP cord-008590-xivnsldf 277 3 sulfate sulfate NN cord-008590-xivnsldf 277 4 ( ( -LRB- cord-008590-xivnsldf 277 5 keratan keratan NNP cord-008590-xivnsldf 277 6 sulfate sulfate NNP cord-008590-xivnsldf 277 7 11 11 CD cord-008590-xivnsldf 277 8 ) ) -RRB- cord-008590-xivnsldf 277 9 is be VBZ cord-008590-xivnsldf 277 10 produced produce VBN cord-008590-xivnsldf 277 11 by by IN cord-008590-xivnsldf 277 12 chrondrocytes chrondrocyte NNS cord-008590-xivnsldf 277 13 of of IN cord-008590-xivnsldf 277 14 mature mature JJ cord-008590-xivnsldf 277 15 cartilage cartilage NN cord-008590-xivnsldf 277 16 and and CC cord-008590-xivnsldf 277 17 contains contain VBZ cord-008590-xivnsldf 277 18 the the DT cord-008590-xivnsldf 277 19 same same JJ cord-008590-xivnsldf 277 20 repeating repeat VBG cord-008590-xivnsldf 277 21 disaccharide disaccharide NN cord-008590-xivnsldf 277 22 as as IN cord-008590-xivnsldf 277 23 the the DT cord-008590-xivnsldf 277 24 N n NN cord-008590-xivnsldf 277 25 - - HYPH cord-008590-xivnsldf 277 26 linked link VBN cord-008590-xivnsldf 277 27 variety variety NN cord-008590-xivnsldf 277 28 ( ( -LRB- cord-008590-xivnsldf 277 29 keratan keratan NNP cord-008590-xivnsldf 277 30 sulfate sulfate NNP cord-008590-xivnsldf 277 31 I I NNP cord-008590-xivnsldf 277 32 ) ) -RRB- cord-008590-xivnsldf 277 33 . . . cord-008590-xivnsldf 278 1 In in IN cord-008590-xivnsldf 278 2 this this DT cord-008590-xivnsldf 278 3 case case NN cord-008590-xivnsldf 278 4 , , , cord-008590-xivnsldf 278 5 the the DT cord-008590-xivnsldf 278 6 saccharide saccharide NN cord-008590-xivnsldf 278 7 is be VBZ cord-008590-xivnsldf 278 8 linked link VBN cord-008590-xivnsldf 278 9 via via IN cord-008590-xivnsldf 278 10 GalNAc galnac NN cord-008590-xivnsldf 278 11 to to IN cord-008590-xivnsldf 278 12 serine serine NN cord-008590-xivnsldf 278 13 or or CC cord-008590-xivnsldf 278 14 threonine threonine NN cord-008590-xivnsldf 278 15 residues residue NNS cord-008590-xivnsldf 278 16 of of IN cord-008590-xivnsldf 278 17 the the DT cord-008590-xivnsldf 278 18 core core NN cord-008590-xivnsldf 278 19 protein protein NN cord-008590-xivnsldf 278 20 . . . cord-008590-xivnsldf 279 1 By by IN cord-008590-xivnsldf 279 2 analogy analogy NN cord-008590-xivnsldf 279 3 with with IN cord-008590-xivnsldf 279 4 data datum NNS cord-008590-xivnsldf 279 5 discussed discuss VBN cord-008590-xivnsldf 279 6 above above RB cord-008590-xivnsldf 279 7 , , , cord-008590-xivnsldf 279 8 one one PRP cord-008590-xivnsldf 279 9 would would MD cord-008590-xivnsldf 279 10 expect expect VB cord-008590-xivnsldf 279 11 both both DT cord-008590-xivnsldf 279 12 chain chain NN cord-008590-xivnsldf 279 13 initiation initiation NN cord-008590-xivnsldf 279 14 and and CC cord-008590-xivnsldf 279 15 elongation elongation NN cord-008590-xivnsldf 279 16 to to TO cord-008590-xivnsldf 279 17 occur occur VB cord-008590-xivnsldf 279 18 in in IN cord-008590-xivnsldf 279 19 the the DT cord-008590-xivnsldf 279 20 GC GC NNP cord-008590-xivnsldf 279 21 . . . cord-008590-xivnsldf 280 1 Since since IN cord-008590-xivnsldf 280 2 the the DT cord-008590-xivnsldf 280 3 same same JJ cord-008590-xivnsldf 280 4 protein protein NN cord-008590-xivnsldf 280 5 core core NN cord-008590-xivnsldf 280 6 contains contain VBZ cord-008590-xivnsldf 280 7 short short JJ cord-008590-xivnsldf 280 8 mucin mucin NN cord-008590-xivnsldf 280 9 - - HYPH cord-008590-xivnsldf 280 10 like like JJ cord-008590-xivnsldf 280 11 O o NN cord-008590-xivnsldf 280 12 - - HYPH cord-008590-xivnsldf 280 13 linked link VBN cord-008590-xivnsldf 280 14 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 280 15 and and CC cord-008590-xivnsldf 280 16 since since IN cord-008590-xivnsldf 280 17 the the DT cord-008590-xivnsldf 280 18 distribution distribution NN cord-008590-xivnsldf 280 19 of of IN cord-008590-xivnsldf 280 20 these these DT cord-008590-xivnsldf 280 21 units unit NNS cord-008590-xivnsldf 280 22 is be VBZ cord-008590-xivnsldf 280 23 not not RB cord-008590-xivnsldf 280 24 random random JJ cord-008590-xivnsldf 280 25 there there EX cord-008590-xivnsldf 280 26 must must MD cord-008590-xivnsldf 280 27 be be VB cord-008590-xivnsldf 280 28 signals signal NNS cord-008590-xivnsldf 280 29 indicating indicate VBG cord-008590-xivnsldf 280 30 which which WDT cord-008590-xivnsldf 280 31 serine serine NN cord-008590-xivnsldf 280 32 or or CC cord-008590-xivnsldf 280 33 threonine threonine NN cord-008590-xivnsldf 280 34 residues residue NNS cord-008590-xivnsldf 280 35 are be VBP cord-008590-xivnsldf 280 36 to to TO cord-008590-xivnsldf 280 37 receive receive VB cord-008590-xivnsldf 280 38 the the DT cord-008590-xivnsldf 280 39 repeating repeat VBG cord-008590-xivnsldf 280 40 disaccharide disaccharide NN cord-008590-xivnsldf 280 41 chains chain NNS cord-008590-xivnsldf 280 42 ( ( -LRB- cord-008590-xivnsldf 280 43 44 44 CD cord-008590-xivnsldf 280 44 , , , cord-008590-xivnsldf 280 45 79 79 CD cord-008590-xivnsldf 280 46 ) ) -RRB- cord-008590-xivnsldf 280 47 . . . cord-008590-xivnsldf 281 1 The the DT cord-008590-xivnsldf 281 2 abundant abundant JJ cord-008590-xivnsldf 281 3 and and CC cord-008590-xivnsldf 281 4 diverse diverse JJ cord-008590-xivnsldf 281 5 classes class NNS cord-008590-xivnsldf 281 6 of of IN cord-008590-xivnsldf 281 7 glycolipids glycolipid NNS cord-008590-xivnsldf 281 8 have have VBP cord-008590-xivnsldf 281 9 received receive VBN cord-008590-xivnsldf 281 10 little little JJ cord-008590-xivnsldf 281 11 attention attention NN cord-008590-xivnsldf 281 12 in in IN cord-008590-xivnsldf 281 13 the the DT cord-008590-xivnsldf 281 14 context context NN cord-008590-xivnsldf 281 15 of of IN cord-008590-xivnsldf 281 16 ICT ICT NNP cord-008590-xivnsldf 281 17 , , , cord-008590-xivnsldf 281 18 although although IN cord-008590-xivnsldf 281 19 glycolipids glycolipid NNS cord-008590-xivnsldf 281 20 are be VBP cord-008590-xivnsldf 281 21 enriched enrich VBN cord-008590-xivnsldf 281 22 in in IN cord-008590-xivnsldf 281 23 plasma plasma NN cord-008590-xivnsldf 281 24 membrane membrane NN cord-008590-xivnsldf 281 25 and and CC cord-008590-xivnsldf 281 26 Golgi golgi NN cord-008590-xivnsldf 281 27 - - HYPH cord-008590-xivnsldf 281 28 rich rich JJ cord-008590-xivnsldf 281 29 fractions fraction NNS cord-008590-xivnsldf 281 30 . . . cord-008590-xivnsldf 282 1 Nevertheless nevertheless RB cord-008590-xivnsldf 282 2 , , , cord-008590-xivnsldf 282 3 a a DT cord-008590-xivnsldf 282 4 unique unique JJ cord-008590-xivnsldf 282 5 elegant elegant JJ cord-008590-xivnsldf 282 6 biosynthetic biosynthetic JJ cord-008590-xivnsldf 282 7 study study NN cord-008590-xivnsldf 282 8 of of IN cord-008590-xivnsldf 282 9 cultured cultured JJ cord-008590-xivnsldf 282 10 neuroblastoma neuroblastoma NN cord-008590-xivnsldf 282 11 cells cell NNS cord-008590-xivnsldf 282 12 shows show VBZ cord-008590-xivnsldf 282 13 that that IN cord-008590-xivnsldf 282 14 several several JJ cord-008590-xivnsldf 282 15 gangliosides ganglioside NNS cord-008590-xivnsldf 282 16 are be VBP cord-008590-xivnsldf 282 17 transported transport VBN cord-008590-xivnsldf 282 18 from from IN cord-008590-xivnsldf 282 19 an an DT cord-008590-xivnsldf 282 20 intracellular intracellular JJ cord-008590-xivnsldf 282 21 site site NN cord-008590-xivnsldf 282 22 ( ( -LRB- cord-008590-xivnsldf 282 23 presumably presumably RB cord-008590-xivnsldf 282 24 the the DT cord-008590-xivnsldf 282 25 GC GC NNP cord-008590-xivnsldf 282 26 ) ) -RRB- cord-008590-xivnsldf 282 27 to to IN cord-008590-xivnsldf 282 28 the the DT cord-008590-xivnsldf 282 29 cell cell NN cord-008590-xivnsldf 282 30 surface surface NN cord-008590-xivnsldf 282 31 with with IN cord-008590-xivnsldf 282 32 a a DT cord-008590-xivnsldf 282 33 half half JJ cord-008590-xivnsldf 282 34 - - HYPH cord-008590-xivnsldf 282 35 time time NN cord-008590-xivnsldf 282 36 of of IN cord-008590-xivnsldf 282 37 20 20 CD cord-008590-xivnsldf 282 38 minutes minute NNS cord-008590-xivnsldf 282 39 ( ( -LRB- cord-008590-xivnsldf 282 40 82 82 CD cord-008590-xivnsldf 282 41 ) ) -RRB- cord-008590-xivnsldf 282 42 . . . cord-008590-xivnsldf 283 1 Following follow VBG cord-008590-xivnsldf 283 2 the the DT cord-008590-xivnsldf 283 3 addition addition NN cord-008590-xivnsldf 283 4 of of IN cord-008590-xivnsldf 283 5 the the DT cord-008590-xivnsldf 283 6 most most RBS cord-008590-xivnsldf 283 7 internal internal JJ cord-008590-xivnsldf 283 8 glucose glucose NN cord-008590-xivnsldf 283 9 or or CC cord-008590-xivnsldf 283 10 galactose galactose NN cord-008590-xivnsldf 283 11 to to IN cord-008590-xivnsldf 283 12 ceramide ceramide NN cord-008590-xivnsldf 283 13 , , , cord-008590-xivnsldf 283 14 a a DT cord-008590-xivnsldf 283 15 broad broad JJ cord-008590-xivnsldf 283 16 range range NN cord-008590-xivnsldf 283 17 of of IN cord-008590-xivnsldf 283 18 further further JJ cord-008590-xivnsldf 283 19 processing processing NN cord-008590-xivnsldf 283 20 pathways pathway NNS cord-008590-xivnsldf 283 21 may may MD cord-008590-xivnsldf 283 22 be be VB cord-008590-xivnsldf 283 23 available available JJ cord-008590-xivnsldf 283 24 , , , cord-008590-xivnsldf 283 25 according accord VBG cord-008590-xivnsldf 283 26 to to IN cord-008590-xivnsldf 283 27 the the DT cord-008590-xivnsldf 283 28 tissue tissue NN cord-008590-xivnsldf 283 29 in in IN cord-008590-xivnsldf 283 30 question question NN cord-008590-xivnsldf 283 31 . . . cord-008590-xivnsldf 284 1 The the DT cord-008590-xivnsldf 284 2 sugar sugar NN cord-008590-xivnsldf 284 3 transferases transferase NNS cord-008590-xivnsldf 284 4 involved involve VBN cord-008590-xivnsldf 284 5 are be VBP cord-008590-xivnsldf 284 6 thought think VBN cord-008590-xivnsldf 284 7 to to TO cord-008590-xivnsldf 284 8 be be VB cord-008590-xivnsldf 284 9 largely largely RB cord-008590-xivnsldf 284 10 distinct distinct JJ cord-008590-xivnsldf 284 11 from from IN cord-008590-xivnsldf 284 12 the the DT cord-008590-xivnsldf 284 13 transferases transferase NNS cord-008590-xivnsldf 284 14 involved involve VBN cord-008590-xivnsldf 284 15 in in IN cord-008590-xivnsldf 284 16 glycoprotein glycoprotein NN cord-008590-xivnsldf 284 17 synthesis synthesis NN cord-008590-xivnsldf 284 18 . . . cord-008590-xivnsldf 285 1 Glycolipid Glycolipid NNP cord-008590-xivnsldf 285 2 sugar sugar NN cord-008590-xivnsldf 285 3 transferases transferase NNS cord-008590-xivnsldf 285 4 responsible responsible JJ cord-008590-xivnsldf 285 5 for for IN cord-008590-xivnsldf 285 6 synthesis synthesis NN cord-008590-xivnsldf 285 7 of of IN cord-008590-xivnsldf 285 8 GM3 GM3 NNP cord-008590-xivnsldf 285 9 , , , cord-008590-xivnsldf 285 10 GM2 GM2 NNP cord-008590-xivnsldf 285 11 , , , cord-008590-xivnsldf 285 12 GMl GMl NNS cord-008590-xivnsldf 285 13 , , , cord-008590-xivnsldf 285 14 and and CC cord-008590-xivnsldf 285 15 GD1 GD1 NNP cord-008590-xivnsldf 285 16 have have VBP cord-008590-xivnsldf 285 17 been be VBN cord-008590-xivnsldf 285 18 found find VBN cord-008590-xivnsldf 285 19 enriched enrich VBN cord-008590-xivnsldf 285 20 in in IN cord-008590-xivnsldf 285 21 preparative preparative JJ cord-008590-xivnsldf 285 22 Golgi Golgi NNP cord-008590-xivnsldf 285 23 fractions fraction NNS cord-008590-xivnsldf 285 24 of of IN cord-008590-xivnsldf 285 25 rat rat NN cord-008590-xivnsldf 285 26 liver liver NN cord-008590-xivnsldf 285 27 , , , cord-008590-xivnsldf 285 28 kidney kidney NN cord-008590-xivnsldf 285 29 , , , cord-008590-xivnsldf 285 30 and and CC cord-008590-xivnsldf 285 31 bovine bovine JJ cord-008590-xivnsldf 285 32 thyroid thyroid NN cord-008590-xivnsldf 285 33 ( ( -LRB- cord-008590-xivnsldf 285 34 22 22 CD cord-008590-xivnsldf 285 35 , , , cord-008590-xivnsldf 285 36 58 58 CD cord-008590-xivnsldf 285 37 , , , cord-008590-xivnsldf 285 38 96 96 CD cord-008590-xivnsldf 285 39 ) ) -RRB- cord-008590-xivnsldf 285 40 ; ; : cord-008590-xivnsldf 285 41 however however RB cord-008590-xivnsldf 285 42 , , , cord-008590-xivnsldf 285 43 it -PRON- PRP cord-008590-xivnsldf 285 44 is be VBZ cord-008590-xivnsldf 285 45 striking strike VBG cord-008590-xivnsldf 285 46 that that IN cord-008590-xivnsldf 285 47 the the DT cord-008590-xivnsldf 285 48 activity activity NN cord-008590-xivnsldf 285 49 responsible responsible JJ cord-008590-xivnsldf 285 50 for for IN cord-008590-xivnsldf 285 51 the the DT cord-008590-xivnsldf 285 52 conversion conversion NN cord-008590-xivnsldf 285 53 of of IN cord-008590-xivnsldf 285 54 GM3 GM3 NNS cord-008590-xivnsldf 285 55 to to IN cord-008590-xivnsldf 285 56 GD3 GD3 NNP cord-008590-xivnsldf 285 57 is be VBZ cord-008590-xivnsldf 285 58 not not RB cord-008590-xivnsldf 285 59 especially especially RB cord-008590-xivnsldf 285 60 enriched enrich VBN cord-008590-xivnsldf 285 61 in in IN cord-008590-xivnsldf 285 62 the the DT cord-008590-xivnsldf 285 63 thyroid thyroid NN cord-008590-xivnsldf 285 64 fractions fraction NNS cord-008590-xivnsldf 285 65 ( ( -LRB- cord-008590-xivnsldf 285 66 Fig Fig NNP cord-008590-xivnsldf 285 67 . . NNP cord-008590-xivnsldf 285 68 4 4 CD cord-008590-xivnsldf 285 69 ) ) -RRB- cord-008590-xivnsldf 285 70 . . . cord-008590-xivnsldf 286 1 Recent recent JJ cord-008590-xivnsldf 286 2 [ [ -LRB- cord-008590-xivnsldf 286 3 3H]Gal 3h]gal CD cord-008590-xivnsldf 286 4 pulse pulse NN cord-008590-xivnsldf 286 5 - - HYPH cord-008590-xivnsldf 286 6 chase chase NN cord-008590-xivnsldf 286 7 studies study NNS cord-008590-xivnsldf 286 8 of of IN cord-008590-xivnsldf 286 9 rat rat NN cord-008590-xivnsldf 286 10 glioma glioma NN cord-008590-xivnsldf 286 11 cells cell NNS cord-008590-xivnsldf 286 12 show show VBP cord-008590-xivnsldf 286 13 a a DT cord-008590-xivnsldf 286 14 precursor precursor NN cord-008590-xivnsldf 286 15 - - HYPH cord-008590-xivnsldf 286 16 product product NN cord-008590-xivnsldf 286 17 relation relation NN cord-008590-xivnsldf 286 18 between between IN cord-008590-xivnsldf 286 19 glucosylceramide glucosylceramide NN cord-008590-xivnsldf 286 20 and and CC cord-008590-xivnsldf 286 21 more more RBR cord-008590-xivnsldf 286 22 complex complex JJ cord-008590-xivnsldf 286 23 glycosphingolipids glycosphingolipid NNS cord-008590-xivnsldf 286 24 . . . cord-008590-xivnsldf 287 1 This this DT cord-008590-xivnsldf 287 2 conversion conversion NN cord-008590-xivnsldf 287 3 is be VBZ cord-008590-xivnsldf 287 4 effectively effectively RB cord-008590-xivnsldf 287 5 blocked block VBN cord-008590-xivnsldf 287 6 by by IN cord-008590-xivnsldf 287 7 monensin monensin NNP cord-008590-xivnsldf 287 8 , , , cord-008590-xivnsldf 287 9 suggesting suggest VBG cord-008590-xivnsldf 287 10 that that IN cord-008590-xivnsldf 287 11 it -PRON- PRP cord-008590-xivnsldf 287 12 involves involve VBZ cord-008590-xivnsldf 287 13 transport transport NN cord-008590-xivnsldf 287 14 from from IN cord-008590-xivnsldf 287 15 the the DT cord-008590-xivnsldf 287 16 early early JJ cord-008590-xivnsldf 287 17 part part NN cord-008590-xivnsldf 287 18 of of IN cord-008590-xivnsldf 287 19 the the DT cord-008590-xivnsldf 287 20 GC GC NNP cord-008590-xivnsldf 287 21 ( ( -LRB- cord-008590-xivnsldf 287 22 or or CC cord-008590-xivnsldf 287 23 RER RER NNP cord-008590-xivnsldf 287 24 ) ) -RRB- cord-008590-xivnsldf 287 25 to to IN cord-008590-xivnsldf 287 26 a a DT cord-008590-xivnsldf 287 27 later later JJ cord-008590-xivnsldf 287 28 region region NN cord-008590-xivnsldf 287 29 . . . cord-008590-xivnsldf 288 1 In in IN cord-008590-xivnsldf 288 2 strict strict JJ cord-008590-xivnsldf 288 3 analogy analogy NN cord-008590-xivnsldf 288 4 with with IN cord-008590-xivnsldf 288 5 studies study NNS cord-008590-xivnsldf 288 6 of of IN cord-008590-xivnsldf 288 7 secretion secretion NN cord-008590-xivnsldf 288 8 of of IN cord-008590-xivnsldf 288 9 [ [ -LRB- cord-008590-xivnsldf 288 10 3H]Gal 3h]gal CD cord-008590-xivnsldf 288 11 - - HYPH cord-008590-xivnsldf 288 12 labeled label VBN cord-008590-xivnsldf 288 13 lg lg NN cord-008590-xivnsldf 288 14 ( ( -LRB- cord-008590-xivnsldf 288 15 139 139 CD cord-008590-xivnsldf 288 16 ) ) -RRB- cord-008590-xivnsldf 288 17 Studies study NNS cord-008590-xivnsldf 288 18 of of IN cord-008590-xivnsldf 288 19 the the DT cord-008590-xivnsldf 288 20 distribution distribution NN cord-008590-xivnsldf 288 21 of of IN cord-008590-xivnsldf 288 22 sulfotransferases sulfotransferase NNS cord-008590-xivnsldf 288 23 involved involve VBN cord-008590-xivnsldf 288 24 in in IN cord-008590-xivnsldf 288 25 sulfatide sulfatide NNP cord-008590-xivnsldf 288 26 synthesis synthesis NN cord-008590-xivnsldf 288 27 have have VBP cord-008590-xivnsldf 288 28 also also RB cord-008590-xivnsldf 288 29 localized localize VBN cord-008590-xivnsldf 288 30 these these DT cord-008590-xivnsldf 288 31 enzymes enzyme NNS cord-008590-xivnsldf 288 32 to to IN cord-008590-xivnsldf 288 33 preparative preparative JJ cord-008590-xivnsldf 288 34 Golgi Golgi NNP cord-008590-xivnsldf 288 35 fractions fraction NNS cord-008590-xivnsldf 288 36 of of IN cord-008590-xivnsldf 288 37 testes testis NNS cord-008590-xivnsldf 288 38 and and CC cord-008590-xivnsldf 288 39 kidney kidney NN cord-008590-xivnsldf 288 40 ( ( -LRB- cord-008590-xivnsldf 288 41 27 27 CD cord-008590-xivnsldf 288 42 ) ) -RRB- cord-008590-xivnsldf 288 43 ( ( -LRB- cord-008590-xivnsldf 288 44 28 28 CD cord-008590-xivnsldf 288 45 ) ) -RRB- cord-008590-xivnsldf 288 46 ( ( -LRB- cord-008590-xivnsldf 288 47 29 29 CD cord-008590-xivnsldf 288 48 ) ) -RRB- cord-008590-xivnsldf 288 49 70 70 CD cord-008590-xivnsldf 288 50 ) ) -RRB- cord-008590-xivnsldf 288 51 . . . cord-008590-xivnsldf 289 1 A a DT cord-008590-xivnsldf 289 2 single single JJ cord-008590-xivnsldf 289 3 study study NN cord-008590-xivnsldf 289 4 demonstrates demonstrate VBZ cord-008590-xivnsldf 289 5 transport transport NN cord-008590-xivnsldf 289 6 of of IN cord-008590-xivnsldf 289 7 sulfatides sulfatide NNS cord-008590-xivnsldf 289 8 from from IN cord-008590-xivnsldf 289 9 GC GC NNP cord-008590-xivnsldf 289 10 to to IN cord-008590-xivnsldf 289 11 the the DT cord-008590-xivnsldf 289 12 cell cell NN cord-008590-xivnsldf 289 13 surface surface NN cord-008590-xivnsldf 289 14 ( ( -LRB- cord-008590-xivnsldf 289 15 28 28 CD cord-008590-xivnsldf 289 16 ) ) -RRB- cord-008590-xivnsldf 289 17 . . . cord-008590-xivnsldf 290 1 Since since IN cord-008590-xivnsldf 290 2 the the DT cord-008590-xivnsldf 290 3 methods method NNS cord-008590-xivnsldf 290 4 for for IN cord-008590-xivnsldf 290 5 detailed detailed JJ cord-008590-xivnsldf 290 6 analysis analysis NN cord-008590-xivnsldf 290 7 of of IN cord-008590-xivnsldf 290 8 glycolipids glycolipid NNS cord-008590-xivnsldf 290 9 are be VBP cord-008590-xivnsldf 290 10 now now RB cord-008590-xivnsldf 290 11 relatively relatively RB cord-008590-xivnsldf 290 12 streamlined streamlined JJ cord-008590-xivnsldf 290 13 and and CC cord-008590-xivnsldf 290 14 since since IN cord-008590-xivnsldf 290 15 the the DT cord-008590-xivnsldf 290 16 biological biological JJ cord-008590-xivnsldf 290 17 importance importance NN cord-008590-xivnsldf 290 18 of of IN cord-008590-xivnsldf 290 19 glycolipids glycolipid NNS cord-008590-xivnsldf 290 20 is be VBZ cord-008590-xivnsldf 290 21 increasingly increasingly RB cord-008590-xivnsldf 290 22 apparent apparent JJ cord-008590-xivnsldf 290 23 , , , cord-008590-xivnsldf 290 24 further further JJ cord-008590-xivnsldf 290 25 data datum NNS cord-008590-xivnsldf 290 26 on on IN cord-008590-xivnsldf 290 27 their -PRON- PRP$ cord-008590-xivnsldf 290 28 ICT ICT NNP cord-008590-xivnsldf 290 29 should should MD cord-008590-xivnsldf 290 30 be be VB cord-008590-xivnsldf 290 31 forthcoming forthcoming JJ cord-008590-xivnsldf 290 32 . . . cord-008590-xivnsldf 291 1 In in IN cord-008590-xivnsldf 291 2 the the DT cord-008590-xivnsldf 291 3 hepatocyte hepatocyte NN cord-008590-xivnsldf 291 4 secretory secretory JJ cord-008590-xivnsldf 291 5 lipoprotein lipoprotein NN cord-008590-xivnsldf 291 6 particles particle NNS cord-008590-xivnsldf 291 7 are be VBP cord-008590-xivnsldf 291 8 visible visible JJ cord-008590-xivnsldf 291 9 within within IN cord-008590-xivnsldf 291 10 the the DT cord-008590-xivnsldf 291 11 smooth smooth JJ cord-008590-xivnsldf 291 12 endoplasmic endoplasmic NN cord-008590-xivnsldf 291 13 reticulum reticulum NN cord-008590-xivnsldf 291 14 and and CC cord-008590-xivnsldf 291 15 become become VB cord-008590-xivnsldf 291 16 conspicuous conspicuous JJ cord-008590-xivnsldf 291 17 within within IN cord-008590-xivnsldf 291 18 the the DT cord-008590-xivnsldf 291 19 GC GC NNP cord-008590-xivnsldf 291 20 , , , cord-008590-xivnsldf 291 21 especially especially RB cord-008590-xivnsldf 291 22 after after IN cord-008590-xivnsldf 291 23 ethanol ethanol NN cord-008590-xivnsldf 291 24 intoxication intoxication NN cord-008590-xivnsldf 291 25 ( ( -LRB- cord-008590-xivnsldf 291 26 1 1 CD cord-008590-xivnsldf 291 27 28 28 CD cord-008590-xivnsldf 291 28 ) ) -RRB- cord-008590-xivnsldf 291 29 . . . cord-008590-xivnsldf 292 1 The the DT cord-008590-xivnsldf 292 2 secreted secrete VBN cord-008590-xivnsldf 292 3 particles particle NNS cord-008590-xivnsldf 292 4 contain contain VBP cord-008590-xivnsldf 292 5 noncovalently noncovalently RB cord-008590-xivnsldf 292 6 bound bind VBN cord-008590-xivnsldf 292 7 triglycerides triglyceride NNS cord-008590-xivnsldf 292 8 , , , cord-008590-xivnsldf 292 9 phospholipids phospholipid NNS cord-008590-xivnsldf 292 10 , , , cord-008590-xivnsldf 292 11 and and CC cord-008590-xivnsldf 292 12 free free JJ cord-008590-xivnsldf 292 13 and and CC cord-008590-xivnsldf 292 14 esterified esterified JJ cord-008590-xivnsldf 292 15 cholesterol cholesterol NN cord-008590-xivnsldf 292 16 . . . cord-008590-xivnsldf 293 1 Recent recent JJ cord-008590-xivnsldf 293 2 studies study NNS cord-008590-xivnsldf 293 3 of of IN cord-008590-xivnsldf 293 4 isolated isolate VBN cord-008590-xivnsldf 293 5 Golgi Golgi NNP cord-008590-xivnsldf 293 6 fractions fraction NNS cord-008590-xivnsldf 293 7 suggest suggest VBP cord-008590-xivnsldf 293 8 that that IN cord-008590-xivnsldf 293 9 the the DT cord-008590-xivnsldf 293 10 phospholipids phospholipid NNS cord-008590-xivnsldf 293 11 of of IN cord-008590-xivnsldf 293 12 the the DT cord-008590-xivnsldf 293 13 contained contain VBN cord-008590-xivnsldf 293 14 lipoproteins lipoprotein NNS cord-008590-xivnsldf 293 15 are be VBP cord-008590-xivnsldf 293 16 in in IN cord-008590-xivnsldf 293 17 rapid rapid JJ cord-008590-xivnsldf 293 18 equilibrium equilibrium NN cord-008590-xivnsldf 293 19 with with IN cord-008590-xivnsldf 293 20 the the DT cord-008590-xivnsldf 293 21 phospholipids phospholipid NNS cord-008590-xivnsldf 293 22 of of IN cord-008590-xivnsldf 293 23 the the DT cord-008590-xivnsldf 293 24 membranes membrane NNS cord-008590-xivnsldf 293 25 of of IN cord-008590-xivnsldf 293 26 Golgi Golgi NNP cord-008590-xivnsldf 293 27 cisternae cisternae NNP cord-008590-xivnsldf 293 28 ( ( -LRB- cord-008590-xivnsldf 293 29 52 52 CD cord-008590-xivnsldf 293 30 ) ) -RRB- cord-008590-xivnsldf 293 31 . . . cord-008590-xivnsldf 294 1 It -PRON- PRP cord-008590-xivnsldf 294 2 therefore therefore RB cord-008590-xivnsldf 294 3 appears appear VBZ cord-008590-xivnsldf 294 4 likely likely JJ cord-008590-xivnsldf 294 5 that that IN cord-008590-xivnsldf 294 6 it -PRON- PRP cord-008590-xivnsldf 294 7 is be VBZ cord-008590-xivnsldf 294 8 within within IN cord-008590-xivnsldf 294 9 the the DT cord-008590-xivnsldf 294 10 GC GC NNP cord-008590-xivnsldf 294 11 that that WDT cord-008590-xivnsldf 294 12 they -PRON- PRP cord-008590-xivnsldf 294 13 acquire acquire VBP cord-008590-xivnsldf 294 14 most most JJS cord-008590-xivnsldf 294 15 of of IN cord-008590-xivnsldf 294 16 their -PRON- PRP$ cord-008590-xivnsldf 294 17 lipids lipid NNS cord-008590-xivnsldf 294 18 . . . cord-008590-xivnsldf 295 1 Nevertheless nevertheless RB cord-008590-xivnsldf 295 2 , , , cord-008590-xivnsldf 295 3 since since IN cord-008590-xivnsldf 295 4 lipoprotein lipoprotein NN cord-008590-xivnsldf 295 5 particles particle NNS cord-008590-xivnsldf 295 6 recovered recover VBN cord-008590-xivnsldf 295 7 from from IN cord-008590-xivnsldf 295 8 rat rat NN cord-008590-xivnsldf 295 9 and and CC cord-008590-xivnsldf 295 10 chicken chicken NN cord-008590-xivnsldf 295 11 liver liver NN cord-008590-xivnsldf 295 12 Golgi Golgi NNP cord-008590-xivnsldf 295 13 fractions fraction NNS cord-008590-xivnsldf 295 14 do do VBP cord-008590-xivnsldf 295 15 not not RB cord-008590-xivnsldf 295 16 have have VB cord-008590-xivnsldf 295 17 the the DT cord-008590-xivnsldf 295 18 same same JJ cord-008590-xivnsldf 295 19 lipid lipid NN cord-008590-xivnsldf 295 20 composition composition NN cord-008590-xivnsldf 295 21 as as IN cord-008590-xivnsldf 295 22 in in IN cord-008590-xivnsldf 295 23 serum serum NN cord-008590-xivnsldf 295 24 , , , cord-008590-xivnsldf 295 25 further further JJ cord-008590-xivnsldf 295 26 intra intra JJ cord-008590-xivnsldf 295 27 - - JJ cord-008590-xivnsldf 295 28 or or CC cord-008590-xivnsldf 295 29 extracellular extracellular JJ cord-008590-xivnsldf 295 30 maturation maturation NN cord-008590-xivnsldf 295 31 events event NNS cord-008590-xivnsldf 295 32 must must MD cord-008590-xivnsldf 295 33 be be VB cord-008590-xivnsldf 295 34 involved involve VBN cord-008590-xivnsldf 295 35 ( ( -LRB- cord-008590-xivnsldf 295 36 4 4 CD cord-008590-xivnsldf 295 37 , , , cord-008590-xivnsldf 295 38 52 52 CD cord-008590-xivnsldf 295 39 ) ) -RRB- cord-008590-xivnsldf 295 40 . . . cord-008590-xivnsldf 296 1 In in IN cord-008590-xivnsldf 296 2 murine murine JJ cord-008590-xivnsldf 296 3 macrophages macrophage NNS cord-008590-xivnsldf 296 4 the the DT cord-008590-xivnsldf 296 5 binding binding NN cord-008590-xivnsldf 296 6 of of IN cord-008590-xivnsldf 296 7 apolipoprotein apolipoprotein NN cord-008590-xivnsldf 296 8 E e NN cord-008590-xivnsldf 296 9 to to IN cord-008590-xivnsldf 296 10 cholesterol cholesterol NN cord-008590-xivnsldf 296 11 occurs occur VBZ cord-008590-xivnsldf 296 12 only only RB cord-008590-xivnsldf 296 13 after after IN cord-008590-xivnsldf 296 14 discharge discharge NN cord-008590-xivnsldf 296 15 from from IN cord-008590-xivnsldf 296 16 the the DT cord-008590-xivnsldf 296 17 cell cell NN cord-008590-xivnsldf 296 18 ( ( -LRB- cord-008590-xivnsldf 296 19 6 6 CD cord-008590-xivnsldf 296 20 ) ) -RRB- cord-008590-xivnsldf 296 21 . . . cord-008590-xivnsldf 297 1 Secretory secretory JJ cord-008590-xivnsldf 297 2 cells cell NNS cord-008590-xivnsldf 297 3 may may MD cord-008590-xivnsldf 297 4 be be VB cord-008590-xivnsldf 297 5 divided divide VBN cord-008590-xivnsldf 297 6 into into IN cord-008590-xivnsldf 297 7 two two CD cord-008590-xivnsldf 297 8 classes class NNS cord-008590-xivnsldf 297 9 : : : cord-008590-xivnsldf 297 10 the the DT cord-008590-xivnsldf 297 11 nonregulated nonregulated JJ cord-008590-xivnsldf 297 12 class class NN cord-008590-xivnsldf 297 13 ( ( -LRB- cord-008590-xivnsldf 297 14 e.g. e.g. RB cord-008590-xivnsldf 297 15 , , , cord-008590-xivnsldf 297 16 plasma plasma NN cord-008590-xivnsldf 297 17 cells cell NNS cord-008590-xivnsldf 297 18 , , , cord-008590-xivnsldf 297 19 fibroblasts fibroblast NNS cord-008590-xivnsldf 297 20 ) ) -RRB- cord-008590-xivnsldf 297 21 and and CC cord-008590-xivnsldf 297 22 the the DT cord-008590-xivnsldf 297 23 regulated regulated JJ cord-008590-xivnsldf 297 24 class class NN cord-008590-xivnsldf 297 25 ( ( -LRB- cord-008590-xivnsldf 297 26 e.g. e.g. RB cord-008590-xivnsldf 297 27 , , , cord-008590-xivnsldf 297 28 pancreatic pancreatic NN cord-008590-xivnsldf 297 29 and and CC cord-008590-xivnsldf 297 30 pituitary pituitary JJ cord-008590-xivnsldf 297 31 cell cell NN cord-008590-xivnsldf 297 32 types type NNS cord-008590-xivnsldf 297 33 ) ) -RRB- cord-008590-xivnsldf 297 34 . . . cord-008590-xivnsldf 298 1 Only only RB cord-008590-xivnsldf 298 2 members member NNS cord-008590-xivnsldf 298 3 of of IN cord-008590-xivnsldf 298 4 the the DT cord-008590-xivnsldf 298 5 regulated regulated JJ cord-008590-xivnsldf 298 6 class class NN cord-008590-xivnsldf 298 7 store store NN cord-008590-xivnsldf 298 8 their -PRON- PRP$ cord-008590-xivnsldf 298 9 secretory secretory JJ cord-008590-xivnsldf 298 10 products product NNS cord-008590-xivnsldf 298 11 in in IN cord-008590-xivnsldf 298 12 granules granule NNS cord-008590-xivnsldf 298 13 . . . cord-008590-xivnsldf 299 1 This this DT cord-008590-xivnsldf 299 2 content content NN cord-008590-xivnsldf 299 3 is be VBZ cord-008590-xivnsldf 299 4 released release VBN cord-008590-xivnsldf 299 5 by by IN cord-008590-xivnsldf 299 6 exocytosis exocytosis NN cord-008590-xivnsldf 299 7 upon upon IN cord-008590-xivnsldf 299 8 physiologic physiologic JJ cord-008590-xivnsldf 299 9 stimulation stimulation NN cord-008590-xivnsldf 299 10 . . . cord-008590-xivnsldf 300 1 The the DT cord-008590-xivnsldf 300 2 genesis genesis NN cord-008590-xivnsldf 300 3 of of IN cord-008590-xivnsldf 300 4 such such JJ cord-008590-xivnsldf 300 5 secretory secretory JJ cord-008590-xivnsldf 300 6 granules granule NNS cord-008590-xivnsldf 300 7 and and CC cord-008590-xivnsldf 300 8 lysosomes lysosome NNS cord-008590-xivnsldf 300 9 takes take VBZ cord-008590-xivnsldf 300 10 place place NN cord-008590-xivnsldf 300 11 either either CC cord-008590-xivnsldf 300 12 within within IN cord-008590-xivnsldf 300 13 distal distal JJ cord-008590-xivnsldf 300 14 Golgi Golgi NNP cord-008590-xivnsldf 300 15 cisternae cisternae NNP cord-008590-xivnsldf 300 16 or or CC cord-008590-xivnsldf 300 17 in in IN cord-008590-xivnsldf 300 18 vacuoles vacuole NNS cord-008590-xivnsldf 300 19 ( ( -LRB- cord-008590-xivnsldf 300 20 e.g. e.g. RB cord-008590-xivnsldf 300 21 , , , cord-008590-xivnsldf 300 22 " " '' cord-008590-xivnsldf 300 23 condensing condense VBG cord-008590-xivnsldf 300 24 vacuoles vacuole NNS cord-008590-xivnsldf 300 25 " " '' cord-008590-xivnsldf 300 26 ) ) -RRB- cord-008590-xivnsldf 300 27 in in IN cord-008590-xivnsldf 300 28 their -PRON- PRP$ cord-008590-xivnsldf 300 29 immediate immediate JJ cord-008590-xivnsldf 300 30 vicinity vicinity NN cord-008590-xivnsldf 300 31 , , , cord-008590-xivnsldf 300 32 according accord VBG cord-008590-xivnsldf 300 33 to to IN cord-008590-xivnsldf 300 34 cell cell NN cord-008590-xivnsldf 300 35 type type NN cord-008590-xivnsldf 300 36 and and CC cord-008590-xivnsldf 300 37 physiologic physiologic JJ cord-008590-xivnsldf 300 38 conditions condition NNS cord-008590-xivnsldf 300 39 ( ( -LRB- cord-008590-xivnsldf 300 40 23 23 CD cord-008590-xivnsldf 300 41 , , , cord-008590-xivnsldf 300 42 24 24 CD cord-008590-xivnsldf 300 43 ) ) -RRB- cord-008590-xivnsldf 300 44 . . . cord-008590-xivnsldf 301 1 Such such JJ cord-008590-xivnsldf 301 2 impressive impressive JJ cord-008590-xivnsldf 301 3 condensation condensation NN cord-008590-xivnsldf 301 4 of of IN cord-008590-xivnsldf 301 5 content content NN cord-008590-xivnsldf 301 6 is be VBZ cord-008590-xivnsldf 301 7 in in IN cord-008590-xivnsldf 301 8 many many JJ cord-008590-xivnsldf 301 9 cases case NNS cord-008590-xivnsldf 301 10 thought think VBN cord-008590-xivnsldf 301 11 to to TO cord-008590-xivnsldf 301 12 reflect reflect VB cord-008590-xivnsldf 301 13 the the DT cord-008590-xivnsldf 301 14 electrostatic electrostatic JJ cord-008590-xivnsldf 301 15 interaction interaction NN cord-008590-xivnsldf 301 16 between between IN cord-008590-xivnsldf 301 17 secretory secretory JJ cord-008590-xivnsldf 301 18 products product NNS cord-008590-xivnsldf 301 19 , , , cord-008590-xivnsldf 301 20 for for IN cord-008590-xivnsldf 301 21 example example NN cord-008590-xivnsldf 301 22 , , , cord-008590-xivnsldf 301 23 between between IN cord-008590-xivnsldf 301 24 secretory secretory JJ cord-008590-xivnsldf 301 25 proteins protein NNS cord-008590-xivnsldf 301 26 and and CC cord-008590-xivnsldf 301 27 proteoglycans proteoglycan NNS cord-008590-xivnsldf 301 28 ( ( -LRB- cord-008590-xivnsldf 301 29 24,77 24,77 CD cord-008590-xivnsldf 301 30 , , , cord-008590-xivnsldf 301 31 142 142 CD cord-008590-xivnsldf 301 32 ) ) -RRB- cord-008590-xivnsldf 301 33 . . . cord-008590-xivnsldf 302 1 Presumably presumably RB cord-008590-xivnsldf 302 2 the the DT cord-008590-xivnsldf 302 3 site site NN cord-008590-xivnsldf 302 4 of of IN cord-008590-xivnsldf 302 5 visible visible JJ cord-008590-xivnsldf 302 6 condensation condensation NN cord-008590-xivnsldf 302 7 corresponds correspond VBZ cord-008590-xivnsldf 302 8 to to IN cord-008590-xivnsldf 302 9 the the DT cord-008590-xivnsldf 302 10 region region NN cord-008590-xivnsldf 302 11 of of IN cord-008590-xivnsldf 302 12 the the DT cord-008590-xivnsldf 302 13 GC GC NNP cord-008590-xivnsldf 302 14 within within IN cord-008590-xivnsldf 302 15 which which WDT cord-008590-xivnsldf 302 16 the the DT cord-008590-xivnsldf 302 17 proteoglycan proteoglycan NN cord-008590-xivnsldf 302 18 is be VBZ cord-008590-xivnsldf 302 19 synthesized synthesize VBN cord-008590-xivnsldf 302 20 ( ( -LRB- cord-008590-xivnsldf 302 21 e.g. e.g. RB cord-008590-xivnsldf 302 22 , , , cord-008590-xivnsldf 302 23 sulfated sulfated JJ cord-008590-xivnsldf 302 24 ) ) -RRB- cord-008590-xivnsldf 302 25 or or CC cord-008590-xivnsldf 302 26 to to IN cord-008590-xivnsldf 302 27 a a DT cord-008590-xivnsldf 302 28 later later JJ cord-008590-xivnsldf 302 29 region region NN cord-008590-xivnsldf 302 30 . . . cord-008590-xivnsldf 303 1 As as IN cord-008590-xivnsldf 303 2 already already RB cord-008590-xivnsldf 303 3 mentioned mention VBN cord-008590-xivnsldf 303 4 , , , cord-008590-xivnsldf 303 5 experiments experiment NNS cord-008590-xivnsldf 303 6 employing employ VBG cord-008590-xivnsldf 303 7 monensin monensin NNP cord-008590-xivnsldf 303 8 indicate indicate VBP cord-008590-xivnsldf 303 9 that that IN cord-008590-xivnsldf 303 10 sulfation sulfation NN cord-008590-xivnsldf 303 11 of of IN cord-008590-xivnsldf 303 12 chondroitin chondroitin NN cord-008590-xivnsldf 303 13 sulfate sulfate NN cord-008590-xivnsldf 303 14 is be VBZ cord-008590-xivnsldf 303 15 a a DT cord-008590-xivnsldf 303 16 rather rather RB cord-008590-xivnsldf 303 17 late late JJ cord-008590-xivnsldf 303 18 Golgi Golgi NNP cord-008590-xivnsldf 303 19 event event NN cord-008590-xivnsldf 303 20 . . . cord-008590-xivnsldf 304 1 The the DT cord-008590-xivnsldf 304 2 proteoglycans proteoglycan NNS cord-008590-xivnsldf 304 3 of of IN cord-008590-xivnsldf 304 4 secretory secretory JJ cord-008590-xivnsldf 304 5 granules granule NNS cord-008590-xivnsldf 304 6 have have VBP cord-008590-xivnsldf 304 7 been be VBN cord-008590-xivnsldf 304 8 reported report VBN cord-008590-xivnsldf 304 9 to to TO cord-008590-xivnsldf 304 10 be be VB cord-008590-xivnsldf 304 11 heparin heparin JJ cord-008590-xivnsldf 304 12 , , , cord-008590-xivnsldf 304 13 heparan heparan NN cord-008590-xivnsldf 304 14 , , , cord-008590-xivnsldf 304 15 dermatan dermatan NNP cord-008590-xivnsldf 304 16 , , , cord-008590-xivnsldf 304 17 and and CC cord-008590-xivnsldf 304 18 chondroitin chondroitin NN cord-008590-xivnsldf 304 19 sulfate sulfate NN cord-008590-xivnsldf 304 20 ( ( -LRB- cord-008590-xivnsldf 304 21 60 60 CD cord-008590-xivnsldf 304 22 , , , cord-008590-xivnsldf 304 23 104 104 CD cord-008590-xivnsldf 304 24 , , , cord-008590-xivnsldf 304 25 142 142 CD cord-008590-xivnsldf 304 26 , , , cord-008590-xivnsldf 304 27 _SP cord-008590-xivnsldf 304 28 150 150 CD cord-008590-xivnsldf 304 29 ) ) -RRB- cord-008590-xivnsldf 304 30 . . . cord-008590-xivnsldf 305 1 A a DT cord-008590-xivnsldf 305 2 fundamental fundamental JJ cord-008590-xivnsldf 305 3 question question NN cord-008590-xivnsldf 305 4 pertaining pertain VBG cord-008590-xivnsldf 305 5 to to IN cord-008590-xivnsldf 305 6 the the DT cord-008590-xivnsldf 305 7 organization organization NN cord-008590-xivnsldf 305 8 of of IN cord-008590-xivnsldf 305 9 the the DT cord-008590-xivnsldf 305 10 GC GC NNP cord-008590-xivnsldf 305 11 should should MD cord-008590-xivnsldf 305 12 also also RB cord-008590-xivnsldf 305 13 be be VB cord-008590-xivnsldf 305 14 considered consider VBN cord-008590-xivnsldf 305 15 . . . cord-008590-xivnsldf 306 1 A a DT cord-008590-xivnsldf 306 2 wealth wealth NN cord-008590-xivnsldf 306 3 of of IN cord-008590-xivnsldf 306 4 data datum NNS cord-008590-xivnsldf 306 5 demonstrates demonstrate VBZ cord-008590-xivnsldf 306 6 that that IN cord-008590-xivnsldf 306 7 the the DT cord-008590-xivnsldf 306 8 terminal terminal JJ cord-008590-xivnsldf 306 9 sugar sugar NN cord-008590-xivnsldf 306 10 transferases transferase NNS cord-008590-xivnsldf 306 11 , , , cord-008590-xivnsldf 306 12 for for IN cord-008590-xivnsldf 306 13 example example NN cord-008590-xivnsldf 306 14 , , , cord-008590-xivnsldf 306 15 are be VBP cord-008590-xivnsldf 306 16 restricted restrict VBN cord-008590-xivnsldf 306 17 to to IN cord-008590-xivnsldf 306 18 a a DT cord-008590-xivnsldf 306 19 subregion subregion NN cord-008590-xivnsldf 306 20 of of IN cord-008590-xivnsldf 306 21 the the DT cord-008590-xivnsldf 306 22 GC GC NNP cord-008590-xivnsldf 306 23 . . . cord-008590-xivnsldf 307 1 Why why WRB cord-008590-xivnsldf 307 2 do do VBP cord-008590-xivnsldf 307 3 these these DT cord-008590-xivnsldf 307 4 proteins protein NNS cord-008590-xivnsldf 307 5 , , , cord-008590-xivnsldf 307 6 synthesized synthesize VBN cord-008590-xivnsldf 307 7 in in IN cord-008590-xivnsldf 307 8 the the DT cord-008590-xivnsldf 307 9 RER rer NN cord-008590-xivnsldf 307 10 , , , cord-008590-xivnsldf 307 11 exit exit NN cord-008590-xivnsldf 307 12 only only RB cord-008590-xivnsldf 307 13 as as RB cord-008590-xivnsldf 307 14 far far RB cord-008590-xivnsldf 307 15 as as IN cord-008590-xivnsldf 307 16 this this DT cord-008590-xivnsldf 307 17 portion portion NN cord-008590-xivnsldf 307 18 of of IN cord-008590-xivnsldf 307 19 the the DT cord-008590-xivnsldf 307 20 GC GC NNP cord-008590-xivnsldf 307 21 and and CC cord-008590-xivnsldf 307 22 migrate migrate VB cord-008590-xivnsldf 307 23 , , , cord-008590-xivnsldf 307 24 if if IN cord-008590-xivnsldf 307 25 at at RB cord-008590-xivnsldf 307 26 all all RB cord-008590-xivnsldf 307 27 , , , cord-008590-xivnsldf 307 28 only only RB cord-008590-xivnsldf 307 29 much much RB cord-008590-xivnsldf 307 30 more more RBR cord-008590-xivnsldf 307 31 slowly slowly RB cord-008590-xivnsldf 307 32 to to IN cord-008590-xivnsldf 307 33 the the DT cord-008590-xivnsldf 307 34 cell cell NN cord-008590-xivnsldf 307 35 surface surface NN cord-008590-xivnsldf 307 36 ? ? . cord-008590-xivnsldf 308 1 Several several JJ cord-008590-xivnsldf 308 2 explanations explanation NNS cord-008590-xivnsldf 308 3 might may MD cord-008590-xivnsldf 308 4 be be VB cord-008590-xivnsldf 308 5 invoked invoke VBN cord-008590-xivnsldf 308 6 : : : cord-008590-xivnsldf 309 1 ( ( -LRB- cord-008590-xivnsldf 309 2 1 1 LS cord-008590-xivnsldf 309 3 ) ) -RRB- cord-008590-xivnsldf 309 4 the the DT cord-008590-xivnsldf 309 5 ionic ionic JJ cord-008590-xivnsldf 309 6 content content NN cord-008590-xivnsldf 309 7 of of IN cord-008590-xivnsldf 309 8 the the DT cord-008590-xivnsldf 309 9 GC GC NNP cord-008590-xivnsldf 309 10 is be VBZ cord-008590-xivnsldf 309 11 unlike unlike IN cord-008590-xivnsldf 309 12 that that IN cord-008590-xivnsldf 309 13 the the DT cord-008590-xivnsldf 309 14 the the DT cord-008590-xivnsldf 309 15 RER RER NNP cord-008590-xivnsldf 309 16 and and CC cord-008590-xivnsldf 309 17 as as IN cord-008590-xivnsldf 309 18 a a DT cord-008590-xivnsldf 309 19 consequence consequence NN cord-008590-xivnsldf 309 20 a a DT cord-008590-xivnsldf 309 21 conformational conformational JJ cord-008590-xivnsldf 309 22 change change NN cord-008590-xivnsldf 309 23 is be VBZ cord-008590-xivnsldf 309 24 induced induce VBN cord-008590-xivnsldf 309 25 in in IN cord-008590-xivnsldf 309 26 such such JJ cord-008590-xivnsldf 309 27 membrane membrane NN cord-008590-xivnsldf 309 28 proteins protein NNS cord-008590-xivnsldf 309 29 which which WDT cord-008590-xivnsldf 309 30 reduces reduce VBZ cord-008590-xivnsldf 309 31 their -PRON- PRP$ cord-008590-xivnsldf 309 32 mobility mobility NN cord-008590-xivnsldf 309 33 - - : cord-008590-xivnsldf 309 34 the the DT cord-008590-xivnsldf 309 35 possibility possibility NN cord-008590-xivnsldf 309 36 that that IN cord-008590-xivnsldf 309 37 the the DT cord-008590-xivnsldf 309 38 Golgi Golgi NNP cord-008590-xivnsldf 309 39 content content NN cord-008590-xivnsldf 309 40 may may MD cord-008590-xivnsldf 309 41 have have VB cord-008590-xivnsldf 309 42 unique unique JJ cord-008590-xivnsldf 309 43 ionic ionic JJ cord-008590-xivnsldf 309 44 properties property NNS cord-008590-xivnsldf 309 45 has have VBZ cord-008590-xivnsldf 309 46 recently recently RB cord-008590-xivnsldf 309 47 been be VBN cord-008590-xivnsldf 309 48 discussed discuss VBN cord-008590-xivnsldf 309 49 ( ( -LRB- cord-008590-xivnsldf 309 50 136 136 CD cord-008590-xivnsldf 309 51 ) ) -RRB- cord-008590-xivnsldf 309 52 , , , cord-008590-xivnsldf 309 53 or or CC cord-008590-xivnsldf 309 54 ( ( -LRB- cord-008590-xivnsldf 309 55 2 2 LS cord-008590-xivnsldf 309 56 ) ) -RRB- cord-008590-xivnsldf 309 57 the the DT cord-008590-xivnsldf 309 58 protein protein NN cord-008590-xivnsldf 309 59 encounters encounter VBZ cord-008590-xivnsldf 309 60 , , , cord-008590-xivnsldf 309 61 within within IN cord-008590-xivnsldf 309 62 the the DT cord-008590-xivnsldf 309 63 membrane membrane NN cord-008590-xivnsldf 309 64 of of IN cord-008590-xivnsldf 309 65 Golgi Golgi NNP cord-008590-xivnsldf 309 66 cisternae cisternae NNP cord-008590-xivnsldf 309 67 , , , cord-008590-xivnsldf 309 68 certain certain JJ cord-008590-xivnsldf 309 69 stable stable JJ cord-008590-xivnsldf 309 70 residents resident NNS cord-008590-xivnsldf 309 71 ( ( -LRB- cord-008590-xivnsldf 309 72 proteins protein NNS cord-008590-xivnsldf 309 73 ? ? . cord-008590-xivnsldf 309 74 ) ) -RRB- cord-008590-xivnsldf 310 1 for for IN cord-008590-xivnsldf 310 2 which which WDT cord-008590-xivnsldf 310 3 it -PRON- PRP cord-008590-xivnsldf 310 4 has have VBZ cord-008590-xivnsldf 310 5 a a DT cord-008590-xivnsldf 310 6 high high JJ cord-008590-xivnsldf 310 7 affinity affinity NN cord-008590-xivnsldf 310 8 , , , cord-008590-xivnsldf 310 9 or or CC cord-008590-xivnsldf 310 10 ( ( -LRB- cord-008590-xivnsldf 310 11 3 3 LS cord-008590-xivnsldf 310 12 ) ) -RRB- cord-008590-xivnsldf 310 13 a a DT cord-008590-xivnsldf 310 14 posttranslational posttranslational JJ cord-008590-xivnsldf 310 15 covalent covalent JJ cord-008590-xivnsldf 310 16 modification modification NN cord-008590-xivnsldf 310 17 executed execute VBN cord-008590-xivnsldf 310 18 within within IN cord-008590-xivnsldf 310 19 the the DT cord-008590-xivnsldf 310 20 GC GC NNP cord-008590-xivnsldf 310 21 is be VBZ cord-008590-xivnsldf 310 22 responsible responsible JJ cord-008590-xivnsldf 310 23 . . . cord-008590-xivnsldf 311 1 To to IN cord-008590-xivnsldf 311 2 date date NN cord-008590-xivnsldf 311 3 , , , cord-008590-xivnsldf 311 4 no no DT cord-008590-xivnsldf 311 5 experimental experimental JJ cord-008590-xivnsldf 311 6 means mean NNS cord-008590-xivnsldf 311 7 have have VBP cord-008590-xivnsldf 311 8 been be VBN cord-008590-xivnsldf 311 9 identified identify VBN cord-008590-xivnsldf 311 10 which which WDT cord-008590-xivnsldf 311 11 cause cause VBP cord-008590-xivnsldf 311 12 mislocalization mislocalization NN cord-008590-xivnsldf 311 13 of of IN cord-008590-xivnsldf 311 14 such such JJ cord-008590-xivnsldf 311 15 Golgi Golgi NNP cord-008590-xivnsldf 311 16 markers marker NNS cord-008590-xivnsldf 311 17 and and CC cord-008590-xivnsldf 311 18 no no DT cord-008590-xivnsldf 311 19 information information NN cord-008590-xivnsldf 311 20 is be VBZ cord-008590-xivnsldf 311 21 available available JJ cord-008590-xivnsldf 311 22 to to TO cord-008590-xivnsldf 311 23 indicate indicate VB cord-008590-xivnsldf 311 24 whether whether IN cord-008590-xivnsldf 311 25 one one CD cord-008590-xivnsldf 311 26 of of IN cord-008590-xivnsldf 311 27 the the DT cord-008590-xivnsldf 311 28 three three CD cord-008590-xivnsldf 311 29 proposed propose VBN cord-008590-xivnsldf 311 30 explanations explanation NNS cord-008590-xivnsldf 311 31 might may MD cord-008590-xivnsldf 311 32 be be VB cord-008590-xivnsldf 311 33 correct correct JJ cord-008590-xivnsldf 311 34 . . . cord-008590-xivnsldf 312 1 The the DT cord-008590-xivnsldf 312 2 point point NN cord-008590-xivnsldf 312 3 of of IN cord-008590-xivnsldf 312 4 departure departure NN cord-008590-xivnsldf 312 5 for for IN cord-008590-xivnsldf 312 6 this this DT cord-008590-xivnsldf 312 7 article article NN cord-008590-xivnsldf 312 8 was be VBD cord-008590-xivnsldf 312 9 the the DT cord-008590-xivnsldf 312 10 supposition supposition NN cord-008590-xivnsldf 312 11 that that IN cord-008590-xivnsldf 312 12 the the DT cord-008590-xivnsldf 312 13 primary primary JJ cord-008590-xivnsldf 312 14 function function NN cord-008590-xivnsldf 312 15 of of IN cord-008590-xivnsldf 312 16 the the DT cord-008590-xivnsldf 312 17 GC GC NNP cord-008590-xivnsldf 312 18 is be VBZ cord-008590-xivnsldf 312 19 to to TO cord-008590-xivnsldf 312 20 accomplish accomplish VB cord-008590-xivnsldf 312 21 net net JJ cord-008590-xivnsldf 312 22 unidirectional unidirectional JJ cord-008590-xivnsldf 312 23 ICT ict NN cord-008590-xivnsldf 312 24 . . . cord-008590-xivnsldf 313 1 It -PRON- PRP cord-008590-xivnsldf 313 2 is be VBZ cord-008590-xivnsldf 313 3 presumably presumably RB cord-008590-xivnsldf 313 4 through through IN cord-008590-xivnsldf 313 5 evolution evolution NN cord-008590-xivnsldf 313 6 that that IN cord-008590-xivnsldf 313 7 the the DT cord-008590-xivnsldf 313 8 GC GC NNP cord-008590-xivnsldf 313 9 has have VBZ cord-008590-xivnsldf 313 10 acquired acquire VBN cord-008590-xivnsldf 313 11 an an DT cord-008590-xivnsldf 313 12 impressive impressive JJ cord-008590-xivnsldf 313 13 array array NN cord-008590-xivnsldf 313 14 of of IN cord-008590-xivnsldf 313 15 other other JJ cord-008590-xivnsldf 313 16 responsibilities responsibility NNS cord-008590-xivnsldf 313 17 . . . cord-008590-xivnsldf 314 1 These these DT cord-008590-xivnsldf 314 2 activities activity NNS cord-008590-xivnsldf 314 3 are be VBP cord-008590-xivnsldf 314 4 the the DT cord-008590-xivnsldf 314 5 subject subject NN cord-008590-xivnsldf 314 6 of of IN cord-008590-xivnsldf 314 7 the the DT cord-008590-xivnsldf 314 8 preceding precede VBG cord-008590-xivnsldf 314 9 pages page NNS cord-008590-xivnsldf 314 10 and and CC cord-008590-xivnsldf 314 11 are be VBP cord-008590-xivnsldf 314 12 summarized summarize VBN cord-008590-xivnsldf 314 13 in in IN cord-008590-xivnsldf 314 14 Table Table NNP cord-008590-xivnsldf 314 15 I -PRON- PRP cord-008590-xivnsldf 314 16 . . . cord-008590-xivnsldf 315 1 What what WP cord-008590-xivnsldf 315 2 is be VBZ cord-008590-xivnsldf 315 3 the the DT cord-008590-xivnsldf 315 4 impact impact NN cord-008590-xivnsldf 315 5 of of IN cord-008590-xivnsldf 315 6 housing housing NN cord-008590-xivnsldf 315 7 these these DT cord-008590-xivnsldf 315 8 processing processing NN cord-008590-xivnsldf 315 9 events event NNS cord-008590-xivnsldf 315 10 on on IN cord-008590-xivnsldf 315 11 the the DT cord-008590-xivnsldf 315 12 GC GC NNP cord-008590-xivnsldf 315 13 , , , cord-008590-xivnsldf 315 14 and and CC cord-008590-xivnsldf 315 15 what what WP cord-008590-xivnsldf 315 16 are be VBP cord-008590-xivnsldf 315 17 some some DT cord-008590-xivnsldf 315 18 of of IN cord-008590-xivnsldf 315 19 the the DT cord-008590-xivnsldf 315 20 dividends dividend NNS cord-008590-xivnsldf 315 21 for for IN cord-008590-xivnsldf 315 22 the the DT cord-008590-xivnsldf 315 23 cell cell NN cord-008590-xivnsldf 315 24 ? ? . cord-008590-xivnsldf 316 1 1 1 LS cord-008590-xivnsldf 316 2 . . . cord-008590-xivnsldf 317 1 The the DT cord-008590-xivnsldf 317 2 GC GC NNP cord-008590-xivnsldf 317 3 has have VBZ cord-008590-xivnsldf 317 4 become become VBN cord-008590-xivnsldf 317 5 such such PDT cord-008590-xivnsldf 317 6 an an DT cord-008590-xivnsldf 317 7 exceedingly exceedingly RB cord-008590-xivnsldf 317 8 active active JJ cord-008590-xivnsldf 317 9 center center NN cord-008590-xivnsldf 317 10 for for IN cord-008590-xivnsldf 317 11 sugar sugar NN cord-008590-xivnsldf 317 12 addition addition NN cord-008590-xivnsldf 317 13 that that IN cord-008590-xivnsldf 317 14 the the DT cord-008590-xivnsldf 317 15 content content NN cord-008590-xivnsldf 317 16 of of IN cord-008590-xivnsldf 317 17 the the DT cord-008590-xivnsldf 317 18 cisternae cisternae NNP cord-008590-xivnsldf 317 19 must must MD cord-008590-xivnsldf 317 20 be be VB cord-008590-xivnsldf 317 21 a a DT cord-008590-xivnsldf 317 22 seething seething JJ cord-008590-xivnsldf 317 23 bath bath NN cord-008590-xivnsldf 317 24 of of IN cord-008590-xivnsldf 317 25 activated activate VBN cord-008590-xivnsldf 317 26 sugar sugar NN cord-008590-xivnsldf 317 27 nucleotides nucleotide NNS cord-008590-xivnsldf 317 28 . . . cord-008590-xivnsldf 318 1 These these DT cord-008590-xivnsldf 318 2 species specie NNS cord-008590-xivnsldf 318 3 , , , cord-008590-xivnsldf 318 4 to to IN cord-008590-xivnsldf 318 5 the the DT cord-008590-xivnsldf 318 6 extent extent NN cord-008590-xivnsldf 318 7 that that IN cord-008590-xivnsldf 318 8 they -PRON- PRP cord-008590-xivnsldf 318 9 have have VBP cord-008590-xivnsldf 318 10 been be VBN cord-008590-xivnsldf 318 11 investigated investigate VBN cord-008590-xivnsldf 318 12 , , , cord-008590-xivnsldf 318 13 gain gain VB cord-008590-xivnsldf 318 14 access access NN cord-008590-xivnsldf 318 15 to to IN cord-008590-xivnsldf 318 16 the the DT cord-008590-xivnsldf 318 17 cisternal cisternal JJ cord-008590-xivnsldf 318 18 space space NN cord-008590-xivnsldf 318 19 by by IN cord-008590-xivnsldf 318 20 facilitated facilitate VBN cord-008590-xivnsldf 318 21 transport transport NN cord-008590-xivnsldf 318 22 through through IN cord-008590-xivnsldf 318 23 specific specific JJ cord-008590-xivnsldf 318 24 sites site NNS cord-008590-xivnsldf 318 25 ( ( -LRB- cord-008590-xivnsldf 318 26 13 13 CD cord-008590-xivnsldf 318 27 , , , cord-008590-xivnsldf 318 28 16 16 CD cord-008590-xivnsldf 318 29 , , , cord-008590-xivnsldf 318 30 30 30 CD cord-008590-xivnsldf 318 31 , , , cord-008590-xivnsldf 318 32 126 126 CD cord-008590-xivnsldf 318 33 ) ) -RRB- cord-008590-xivnsldf 318 34 . . . cord-008590-xivnsldf 319 1 This this DT cord-008590-xivnsldf 319 2 set set NN cord-008590-xivnsldf 319 3 of of IN cord-008590-xivnsldf 319 4 transporters transporter NNS cord-008590-xivnsldf 319 5 may may MD cord-008590-xivnsldf 319 6 be be VB cord-008590-xivnsldf 319 7 considered consider VBN cord-008590-xivnsldf 319 8 as as IN cord-008590-xivnsldf 319 9 additional additional JJ cord-008590-xivnsldf 319 10 set set NN cord-008590-xivnsldf 319 11 of of IN cord-008590-xivnsldf 319 12 Golgi Golgi NNP cord-008590-xivnsldf 319 13 markers marker NNS cord-008590-xivnsldf 319 14 . . . cord-008590-xivnsldf 320 1 2 2 LS cord-008590-xivnsldf 320 2 . . . cord-008590-xivnsldf 321 1 The the DT cord-008590-xivnsldf 321 2 GC GC NNP cord-008590-xivnsldf 321 3 must must MD cord-008590-xivnsldf 321 4 deal deal VB cord-008590-xivnsldf 321 5 with with IN cord-008590-xivnsldf 321 6 the the DT cord-008590-xivnsldf 321 7 side side NN cord-008590-xivnsldf 321 8 products product NNS cord-008590-xivnsldf 321 9 of of IN cord-008590-xivnsldf 321 10 these these DT cord-008590-xivnsldf 321 11 reactions reaction NNS cord-008590-xivnsldf 321 12 - - : cord-008590-xivnsldf 321 13 the the DT cord-008590-xivnsldf 321 14 mono mono NN cord-008590-xivnsldf 321 15 - - HYPH cord-008590-xivnsldf 321 16 and and CC cord-008590-xivnsldf 321 17 dinucleosides dinucleoside NNS cord-008590-xivnsldf 321 18 which which WDT cord-008590-xivnsldf 321 19 are be VBP cord-008590-xivnsldf 321 20 produced produce VBN cord-008590-xivnsldf 321 21 by by IN cord-008590-xivnsldf 321 22 the the DT cord-008590-xivnsldf 321 23 transferases transferase NNS cord-008590-xivnsldf 321 24 - - : cord-008590-xivnsldf 321 25 especially especially RB cord-008590-xivnsldf 321 26 since since IN cord-008590-xivnsldf 321 27 UDP udp NN cord-008590-xivnsldf 321 28 inhibits inhibit VBZ cord-008590-xivnsldf 321 29 GalNAc GalNAc NNP cord-008590-xivnsldf 321 30 and and CC cord-008590-xivnsldf 321 31 Gal Gal NNP cord-008590-xivnsldf 321 32 transferase transferase NN cord-008590-xivnsldf 321 33 hyaluronate hyaluronate VBZ cord-008590-xivnsldf 321 34 synthetase synthetase NN cord-008590-xivnsldf 321 35 ( ( -LRB- cord-008590-xivnsldf 321 36 59,95 59,95 CD cord-008590-xivnsldf 321 37 , , , cord-008590-xivnsldf 321 38 100b 100b NNP cord-008590-xivnsldf 321 39 ) ) -RRB- cord-008590-xivnsldf 321 40 . . . cord-008590-xivnsldf 322 1 Both both DT cord-008590-xivnsldf 322 2 the the DT cord-008590-xivnsldf 322 3 UDP udp NN cord-008590-xivnsldf 322 4 and and CC cord-008590-xivnsldf 322 5 GDP GDP NNP cord-008590-xivnsldf 322 6 and and CC cord-008590-xivnsldf 322 7 to to IN cord-008590-xivnsldf 322 8 a a DT cord-008590-xivnsldf 322 9 lesser less JJR cord-008590-xivnsldf 322 10 extent extent NN cord-008590-xivnsldf 322 11 CMP cmp NN cord-008590-xivnsldf 322 12 are be VBP cord-008590-xivnsldf 322 13 broken break VBN cord-008590-xivnsldf 322 14 down down RP cord-008590-xivnsldf 322 15 within within IN cord-008590-xivnsldf 322 16 the the DT cord-008590-xivnsldf 322 17 cisternal cisternal JJ cord-008590-xivnsldf 322 18 space space NN cord-008590-xivnsldf 322 19 by by IN cord-008590-xivnsldf 322 20 nucleoside nucleoside NN cord-008590-xivnsldf 322 21 diphosphatase diphosphatase NN cord-008590-xivnsldf 322 22 ( ( -LRB- cord-008590-xivnsldf 322 23 9 9 CD cord-008590-xivnsldf 322 24 , , , cord-008590-xivnsldf 322 25 95 95 CD cord-008590-xivnsldf 322 26 ) ) -RRB- cord-008590-xivnsldf 322 27 . . . cord-008590-xivnsldf 323 1 Furthermore furthermore RB cord-008590-xivnsldf 323 2 , , , cord-008590-xivnsldf 323 3 the the DT cord-008590-xivnsldf 323 4 end end NN cord-008590-xivnsldf 323 5 - - HYPH cord-008590-xivnsldf 323 6 products product NNS cord-008590-xivnsldf 323 7 of of IN cord-008590-xivnsldf 323 8 hydrolysis hydrolysis NN cord-008590-xivnsldf 323 9 must must MD cord-008590-xivnsldf 323 10 exit exit VB cord-008590-xivnsldf 323 11 from from IN cord-008590-xivnsldf 323 12 the the DT cord-008590-xivnsldf 323 13 cisternae cisternae NNP cord-008590-xivnsldf 323 14 in in IN cord-008590-xivnsldf 323 15 order order NN cord-008590-xivnsldf 323 16 to to TO cord-008590-xivnsldf 323 17 function function VB cord-008590-xivnsldf 323 18 in in IN cord-008590-xivnsldf 323 19 repetitive repetitive JJ cord-008590-xivnsldf 323 20 cycles cycle NNS cord-008590-xivnsldf 323 21 . . . cord-008590-xivnsldf 324 1 Only only RB cord-008590-xivnsldf 324 2 a a DT cord-008590-xivnsldf 324 3 single single JJ cord-008590-xivnsldf 324 4 report report NN cord-008590-xivnsldf 324 5 pertains pertain VBZ cord-008590-xivnsldf 324 6 to to IN cord-008590-xivnsldf 324 7 this this DT cord-008590-xivnsldf 324 8 second second JJ cord-008590-xivnsldf 324 9 class class NN cord-008590-xivnsldf 324 10 of of IN cord-008590-xivnsldf 324 11 carriers carrier NNS cord-008590-xivnsldf 324 12 ( ( -LRB- cord-008590-xivnsldf 324 13 30 30 CD cord-008590-xivnsldf 324 14 ) ) -RRB- cord-008590-xivnsldf 324 15 . . . cord-008590-xivnsldf 325 1 In in IN cord-008590-xivnsldf 325 2 the the DT cord-008590-xivnsldf 325 3 same same JJ cord-008590-xivnsldf 325 4 vein vein NN cord-008590-xivnsldf 325 5 , , , cord-008590-xivnsldf 325 6 the the DT cord-008590-xivnsldf 325 7 ability ability NN cord-008590-xivnsldf 325 8 of of IN cord-008590-xivnsldf 325 9 the the DT cord-008590-xivnsldf 325 10 GC GC NNP cord-008590-xivnsldf 325 11 to to TO cord-008590-xivnsldf 325 12 synthesize synthesize VB cord-008590-xivnsldf 325 13 lactose lactose NN cord-008590-xivnsldf 325 14 ( ( -LRB- cord-008590-xivnsldf 325 15 in in IN cord-008590-xivnsldf 325 16 the the DT cord-008590-xivnsldf 325 17 mammary mammary JJ cord-008590-xivnsldf 325 18 gland gland NN cord-008590-xivnsldf 325 19 ) ) -RRB- cord-008590-xivnsldf 325 20 and and CC cord-008590-xivnsldf 325 21 the the DT cord-008590-xivnsldf 325 22 presence presence NN cord-008590-xivnsldf 325 23 of of IN cord-008590-xivnsldf 325 24 both both DT cord-008590-xivnsldf 325 25 mannosidase mannosidase NN cord-008590-xivnsldf 325 26 and and CC cord-008590-xivnsldf 325 27 phosphodiesterase phosphodiesterase NN cord-008590-xivnsldf 325 28 activities activity NNS cord-008590-xivnsldf 325 29 suggests suggest VBZ cord-008590-xivnsldf 325 30 that that IN cord-008590-xivnsldf 325 31 Golgi Golgi NNP cord-008590-xivnsldf 325 32 membranes membrane NNS cord-008590-xivnsldf 325 33 are be VBP cord-008590-xivnsldf 325 34 impermeable impermeable JJ cord-008590-xivnsldf 325 35 to to IN cord-008590-xivnsldf 325 36 disaccharides disaccharide NNS cord-008590-xivnsldf 325 37 yet yet RB cord-008590-xivnsldf 325 38 permeable permeable JJ cord-008590-xivnsldf 325 39 to to IN cord-008590-xivnsldf 325 40 monosaccharides monosaccharide NNS cord-008590-xivnsldf 325 41 . . . cord-008590-xivnsldf 326 1 Experimental experimental JJ cord-008590-xivnsldf 326 2 observations observation NNS cord-008590-xivnsldf 326 3 on on IN cord-008590-xivnsldf 326 4 the the DT cord-008590-xivnsldf 326 5 mammary mammary JJ cord-008590-xivnsldf 326 6 gland gland NN cord-008590-xivnsldf 326 7 and and CC cord-008590-xivnsldf 326 8 myeloma myeloma NN cord-008590-xivnsldf 326 9 cells cell NNS cord-008590-xivnsldf 326 10 suggest suggest VBP cord-008590-xivnsldf 326 11 that that IN cord-008590-xivnsldf 326 12 this this DT cord-008590-xivnsldf 326 13 is be VBZ cord-008590-xivnsldf 326 14 the the DT cord-008590-xivnsldf 326 15 case case NN cord-008590-xivnsldf 326 16 ( ( -LRB- cord-008590-xivnsldf 326 17 135 135 CD cord-008590-xivnsldf 326 18 , , , cord-008590-xivnsldf 326 19 145 145 CD cord-008590-xivnsldf 326 20 ) ) -RRB- cord-008590-xivnsldf 326 21 . . . cord-008590-xivnsldf 327 1 3 3 LS cord-008590-xivnsldf 327 2 . . . cord-008590-xivnsldf 328 1 The the DT cord-008590-xivnsldf 328 2 ability ability NN cord-008590-xivnsldf 328 3 to to TO cord-008590-xivnsldf 328 4 assemble assemble VB cord-008590-xivnsldf 328 5 proteoglycans proteoglycan NNS cord-008590-xivnsldf 328 6 may may MD cord-008590-xivnsldf 328 7 be be VB cord-008590-xivnsldf 328 8 considered consider VBN cord-008590-xivnsldf 328 9 a a DT cord-008590-xivnsldf 328 10 prerequisite prerequisite NN cord-008590-xivnsldf 328 11 for for IN cord-008590-xivnsldf 328 12 the the DT cord-008590-xivnsldf 328 13 existence existence NN cord-008590-xivnsldf 328 14 of of IN cord-008590-xivnsldf 328 15 the the DT cord-008590-xivnsldf 328 16 regulated regulate VBN cord-008590-xivnsldf 328 17 secretory secretory JJ cord-008590-xivnsldf 328 18 cell cell NN cord-008590-xivnsldf 328 19 phenotype phenotype NN cord-008590-xivnsldf 328 20 . . . cord-008590-xivnsldf 329 1 4 4 LS cord-008590-xivnsldf 329 2 . . . cord-008590-xivnsldf 330 1 The the DT cord-008590-xivnsldf 330 2 addition addition NN cord-008590-xivnsldf 330 3 of of IN cord-008590-xivnsldf 330 4 both both DT cord-008590-xivnsldf 330 5 neutral neutral JJ cord-008590-xivnsldf 330 6 and and CC cord-008590-xivnsldf 330 7 negatively negatively RB cord-008590-xivnsldf 330 8 charged charge VBN cord-008590-xivnsldf 330 9 sugars sugar NNS cord-008590-xivnsldf 330 10 to to IN cord-008590-xivnsldf 330 11 intrisic intrisic JJ cord-008590-xivnsldf 330 12 glycoproteins glycoprotein NNS cord-008590-xivnsldf 330 13 and and CC cord-008590-xivnsldf 330 14 glycolipids glycolipid NNS cord-008590-xivnsldf 330 15 of of IN cord-008590-xivnsldf 330 16 the the DT cord-008590-xivnsldf 330 17 GC GC NNP cord-008590-xivnsldf 330 18 obviously obviously RB cord-008590-xivnsldf 330 19 modifies modify VBZ cord-008590-xivnsldf 330 20 the the DT cord-008590-xivnsldf 330 21 environment environment NN cord-008590-xivnsldf 330 22 in in IN cord-008590-xivnsldf 330 23 which which WDT cord-008590-xivnsldf 330 24 other other JJ cord-008590-xivnsldf 330 25 Golgi Golgi NNP cord-008590-xivnsldf 330 26 activities activity NNS cord-008590-xivnsldf 330 27 occur occur VBP cord-008590-xivnsldf 330 28 . . . cord-008590-xivnsldf 331 1 For for IN cord-008590-xivnsldf 331 2 example example NN cord-008590-xivnsldf 331 3 , , , cord-008590-xivnsldf 331 4 the the DT cord-008590-xivnsldf 331 5 local local JJ cord-008590-xivnsldf 331 6 membrane membrane NN cord-008590-xivnsldf 331 7 potential potential NN cord-008590-xivnsldf 331 8 may may MD cord-008590-xivnsldf 331 9 be be VB cord-008590-xivnsldf 331 10 perturbed perturb VBN cord-008590-xivnsldf 331 11 at at IN cord-008590-xivnsldf 331 12 sites site NNS cord-008590-xivnsldf 331 13 where where WRB cord-008590-xivnsldf 331 14 sialic sialic JJ cord-008590-xivnsldf 331 15 acid acid NN cord-008590-xivnsldf 331 16 has have VBZ cord-008590-xivnsldf 331 17 been be VBN cord-008590-xivnsldf 331 18 added add VBN cord-008590-xivnsldf 331 19 , , , cord-008590-xivnsldf 331 20 and and CC cord-008590-xivnsldf 331 21 the the DT cord-008590-xivnsldf 331 22 presence presence NN cord-008590-xivnsldf 331 23 of of IN cord-008590-xivnsldf 331 24 ceramide ceramide NN cord-008590-xivnsldf 331 25 derivatives derivative NNS cord-008590-xivnsldf 331 26 may may MD cord-008590-xivnsldf 331 27 result result VB cord-008590-xivnsldf 331 28 in in IN cord-008590-xivnsldf 331 29 membrane membrane NN cord-008590-xivnsldf 331 30 rigidification rigidification NN cord-008590-xivnsldf 331 31 ( ( -LRB- cord-008590-xivnsldf 331 32 148 148 CD cord-008590-xivnsldf 331 33 ) ) -RRB- cord-008590-xivnsldf 331 34 . . . cord-008590-xivnsldf 332 1 The the DT cord-008590-xivnsldf 332 2 assembly assembly NN cord-008590-xivnsldf 332 3 of of IN cord-008590-xivnsldf 332 4 membrane membrane NN cord-008590-xivnsldf 332 5 - - HYPH cord-008590-xivnsldf 332 6 bound bind VBN cord-008590-xivnsldf 332 7 oligosaccharides oligosaccharide NNS cord-008590-xivnsldf 332 8 clearly clearly RB cord-008590-xivnsldf 332 9 allows allow VBZ cord-008590-xivnsldf 332 10 the the DT cord-008590-xivnsldf 332 11 cell cell NN cord-008590-xivnsldf 332 12 to to TO cord-008590-xivnsldf 332 13 endow endow VB cord-008590-xivnsldf 332 14 its -PRON- PRP$ cord-008590-xivnsldf 332 15 surface surface NN cord-008590-xivnsldf 332 16 with with IN cord-008590-xivnsldf 332 17 negative negative JJ cord-008590-xivnsldf 332 18 charge charge NN cord-008590-xivnsldf 332 19 and and CC cord-008590-xivnsldf 332 20 an an DT cord-008590-xivnsldf 332 21 array array NN cord-008590-xivnsldf 332 22 of of IN cord-008590-xivnsldf 332 23 specific specific JJ cord-008590-xivnsldf 332 24 structural structural JJ cord-008590-xivnsldf 332 25 sites site NNS cord-008590-xivnsldf 332 26 beyond beyond IN cord-008590-xivnsldf 332 27 those those DT cord-008590-xivnsldf 332 28 simply simply RB cord-008590-xivnsldf 332 29 encoded encode VBN cord-008590-xivnsldf 332 30 in in IN cord-008590-xivnsldf 332 31 its -PRON- PRP$ cord-008590-xivnsldf 332 32 polypeptides polypeptide NNS cord-008590-xivnsldf 332 33 . . . cord-008590-xivnsldf 333 1 6 6 LS cord-008590-xivnsldf 333 2 . . . cord-008590-xivnsldf 334 1 The the DT cord-008590-xivnsldf 334 2 generation generation NN cord-008590-xivnsldf 334 3 of of IN cord-008590-xivnsldf 334 4 mannose mannose NN cord-008590-xivnsldf 334 5 phosphate phosphate NN cord-008590-xivnsldf 334 6 groups group NNS cord-008590-xivnsldf 334 7 , , , cord-008590-xivnsldf 334 8 in in IN cord-008590-xivnsldf 334 9 many many JJ cord-008590-xivnsldf 334 10 cells cell NNS cord-008590-xivnsldf 334 11 , , , cord-008590-xivnsldf 334 12 is be VBZ cord-008590-xivnsldf 334 13 essential essential JJ cord-008590-xivnsldf 334 14 for for IN cord-008590-xivnsldf 334 15 targeting target VBG cord-008590-xivnsldf 334 16 of of IN cord-008590-xivnsldf 334 17 lysosomal lysosomal JJ cord-008590-xivnsldf 334 18 enzymes enzyme NNS cord-008590-xivnsldf 334 19 . . . cord-008590-xivnsldf 335 1 Such such JJ cord-008590-xivnsldf 335 2 considerations consideration NNS cord-008590-xivnsldf 335 3 indicate indicate VBP cord-008590-xivnsldf 335 4 that that IN cord-008590-xivnsldf 335 5 the the DT cord-008590-xivnsldf 335 6 existence existence NN cord-008590-xivnsldf 335 7 of of IN cord-008590-xivnsldf 335 8 posttranslational posttranslational JJ cord-008590-xivnsldf 335 9 processing processing NN cord-008590-xivnsldf 335 10 events event NNS cord-008590-xivnsldf 335 11 is be VBZ cord-008590-xivnsldf 335 12 an an DT cord-008590-xivnsldf 335 13 integral integral JJ cord-008590-xivnsldf 335 14 part part NN cord-008590-xivnsldf 335 15 of of IN cord-008590-xivnsldf 335 16 the the DT cord-008590-xivnsldf 335 17 cells cell NNS cord-008590-xivnsldf 335 18 repertoire repertoire NN cord-008590-xivnsldf 335 19 of of IN cord-008590-xivnsldf 335 20 biosynthetic biosynthetic JJ cord-008590-xivnsldf 335 21 equipment equipment NN cord-008590-xivnsldf 335 22 . . . cord-008590-xivnsldf 336 1 Given give VBN cord-008590-xivnsldf 336 2 this this DT cord-008590-xivnsldf 336 3 realization realization NN cord-008590-xivnsldf 336 4 , , , cord-008590-xivnsldf 336 5 it -PRON- PRP cord-008590-xivnsldf 336 6 would would MD cord-008590-xivnsldf 336 7 not not RB cord-008590-xivnsldf 336 8 be be VB cord-008590-xivnsldf 336 9 altogether altogether RB cord-008590-xivnsldf 336 10 suprising suprising JJ cord-008590-xivnsldf 336 11 to to TO cord-008590-xivnsldf 336 12 learn learn VB cord-008590-xivnsldf 336 13 that that DT cord-008590-xivnsldf 336 14 nucleoside nucleoside NN cord-008590-xivnsldf 336 15 diphosphatase diphosphatase NN cord-008590-xivnsldf 336 16 , , , cord-008590-xivnsldf 336 17 for for IN cord-008590-xivnsldf 336 18 example example NN cord-008590-xivnsldf 336 19 , , , cord-008590-xivnsldf 336 20 had have VBD cord-008590-xivnsldf 336 21 subsumed subsume VBN cord-008590-xivnsldf 336 22 certain certain JJ cord-008590-xivnsldf 336 23 functions function NNS cord-008590-xivnsldf 336 24 at at IN cord-008590-xivnsldf 336 25 the the DT cord-008590-xivnsldf 336 26 level level NN cord-008590-xivnsldf 336 27 or or CC cord-008590-xivnsldf 336 28 overall overall JJ cord-008590-xivnsldf 336 29 Golgi Golgi NNP cord-008590-xivnsldf 336 30 structrue structrue NN cord-008590-xivnsldf 336 31 and and CC cord-008590-xivnsldf 336 32 organization organization NN cord-008590-xivnsldf 336 33 . . . cord-008590-xivnsldf 337 1 Other other JJ cord-008590-xivnsldf 337 2 proteins protein NNS cord-008590-xivnsldf 337 3 involved involve VBN cord-008590-xivnsldf 337 4 in in IN cord-008590-xivnsldf 337 5 more more RBR cord-008590-xivnsldf 337 6 specialized specialized JJ cord-008590-xivnsldf 337 7 processing processing NN cord-008590-xivnsldf 337 8 events event NNS cord-008590-xivnsldf 337 9 , , , cord-008590-xivnsldf 337 10 e.g. e.g. RB cord-008590-xivnsldf 337 11 , , , cord-008590-xivnsldf 337 12 select select JJ cord-008590-xivnsldf 337 13 glycolipid glycolipid NNP cord-008590-xivnsldf 337 14 sugar sugar NN cord-008590-xivnsldf 337 15 transferases transferase NNS cord-008590-xivnsldf 337 16 , , , cord-008590-xivnsldf 337 17 found find VBD cord-008590-xivnsldf 337 18 only only RB cord-008590-xivnsldf 337 19 in in IN cord-008590-xivnsldf 337 20 the the DT cord-008590-xivnsldf 337 21 GC GC NNP cord-008590-xivnsldf 337 22 of of IN cord-008590-xivnsldf 337 23 certain certain JJ cord-008590-xivnsldf 337 24 cells cell NNS cord-008590-xivnsldf 337 25 , , , cord-008590-xivnsldf 337 26 might may MD cord-008590-xivnsldf 337 27 not not RB cord-008590-xivnsldf 337 28 be be VB cord-008590-xivnsldf 337 29 expected expect VBN cord-008590-xivnsldf 337 30 to to TO cord-008590-xivnsldf 337 31 have have VB cord-008590-xivnsldf 337 32 acquired acquire VBN cord-008590-xivnsldf 337 33 such such JJ cord-008590-xivnsldf 337 34 dual dual JJ cord-008590-xivnsldf 337 35 responsibilities responsibility NNS cord-008590-xivnsldf 337 36 . . . cord-008590-xivnsldf 338 1 Other other JJ cord-008590-xivnsldf 338 2 common common JJ cord-008590-xivnsldf 338 3 features feature NNS cord-008590-xivnsldf 338 4 of of IN cord-008590-xivnsldf 338 5 processing processing NN cord-008590-xivnsldf 338 6 deserve deserve VBP cord-008590-xivnsldf 338 7 comment comment NN cord-008590-xivnsldf 338 8 , , , cord-008590-xivnsldf 338 9 for for IN cord-008590-xivnsldf 338 10 example example NN cord-008590-xivnsldf 338 11 , , , cord-008590-xivnsldf 338 12 the the DT cord-008590-xivnsldf 338 13 microheterogeneity microheterogeneity NN cord-008590-xivnsldf 338 14 which which WDT cord-008590-xivnsldf 338 15 is be VBZ cord-008590-xivnsldf 338 16 commonplace commonplace JJ cord-008590-xivnsldf 338 17 among among IN cord-008590-xivnsldf 338 18 glycoproteins glycoprotein NNS cord-008590-xivnsldf 338 19 and and CC cord-008590-xivnsldf 338 20 may may MD cord-008590-xivnsldf 338 21 also also RB cord-008590-xivnsldf 338 22 be be VB cord-008590-xivnsldf 338 23 characteristic characteristic JJ cord-008590-xivnsldf 338 24 of of IN cord-008590-xivnsldf 338 25 proteolytic proteolytic JJ cord-008590-xivnsldf 338 26 cleavage cleavage NN cord-008590-xivnsldf 338 27 . . . cord-008590-xivnsldf 339 1 No no DT cord-008590-xivnsldf 339 2 experimental experimental JJ cord-008590-xivnsldf 339 3 studies study NNS cord-008590-xivnsldf 339 4 have have VBP cord-008590-xivnsldf 339 5 attempted attempt VBN cord-008590-xivnsldf 339 6 to to TO cord-008590-xivnsldf 339 7 manipulate manipulate VB cord-008590-xivnsldf 339 8 the the DT cord-008590-xivnsldf 339 9 degree degree NN cord-008590-xivnsldf 339 10 of of IN cord-008590-xivnsldf 339 11 microheterogeneity microheterogeneity NN cord-008590-xivnsldf 339 12 , , , cord-008590-xivnsldf 339 13 e e NNP cord-008590-xivnsldf 339 14 .g .g NNP cord-008590-xivnsldf 339 15 . . . cord-008590-xivnsldf 339 16 , , , cord-008590-xivnsldf 339 17 by by IN cord-008590-xivnsldf 339 18 reducing reduce VBG cord-008590-xivnsldf 339 19 the the DT cord-008590-xivnsldf 339 20 temperature temperature NN cord-008590-xivnsldf 339 21 so so IN cord-008590-xivnsldf 339 22 as as IN cord-008590-xivnsldf 339 23 to to TO cord-008590-xivnsldf 339 24 slow slow VB cord-008590-xivnsldf 339 25 transport transport NN cord-008590-xivnsldf 339 26 through through IN cord-008590-xivnsldf 339 27 the the DT cord-008590-xivnsldf 339 28 GC GC NNP cord-008590-xivnsldf 339 29 . . . cord-008590-xivnsldf 340 1 Nevertheless nevertheless RB cord-008590-xivnsldf 340 2 , , , cord-008590-xivnsldf 340 3 in in IN cord-008590-xivnsldf 340 4 addition addition NN cord-008590-xivnsldf 340 5 to to IN cord-008590-xivnsldf 340 6 the the DT cord-008590-xivnsldf 340 7 enzymologic enzymologic JJ cord-008590-xivnsldf 340 8 considerations consideration NNS cord-008590-xivnsldf 340 9 mentioned mention VBN cord-008590-xivnsldf 340 10 above above RB cord-008590-xivnsldf 340 11 ( ( -LRB- cord-008590-xivnsldf 340 12 overlapping overlap VBG cord-008590-xivnsldf 340 13 transferase transferase NN cord-008590-xivnsldf 340 14 specificities specificity NNS cord-008590-xivnsldf 340 15 , , , cord-008590-xivnsldf 340 16 etc etc FW cord-008590-xivnsldf 340 17 . . . cord-008590-xivnsldf 340 18 ) ) -RRB- cord-008590-xivnsldf 340 19 , , , cord-008590-xivnsldf 340 20 one one NN cord-008590-xivnsldf 340 21 wonders wonder VBZ cord-008590-xivnsldf 340 22 whether whether IN cord-008590-xivnsldf 340 23 dispersion dispersion NN cord-008590-xivnsldf 340 24 in in IN cord-008590-xivnsldf 340 25 the the DT cord-008590-xivnsldf 340 26 rates rate NNS cord-008590-xivnsldf 340 27 of of IN cord-008590-xivnsldf 340 28 entry entry NN cord-008590-xivnsldf 340 29 and and CC cord-008590-xivnsldf 340 30 exit exit NN cord-008590-xivnsldf 340 31 and and CC cord-008590-xivnsldf 340 32 duration duration NN cord-008590-xivnsldf 340 33 of of IN cord-008590-xivnsldf 340 34 residence residence NN cord-008590-xivnsldf 340 35 within within IN cord-008590-xivnsldf 340 36 a a DT cord-008590-xivnsldf 340 37 given give VBN cord-008590-xivnsldf 340 38 subcompartment subcompartment NN cord-008590-xivnsldf 340 39 may may MD cord-008590-xivnsldf 340 40 not not RB cord-008590-xivnsldf 340 41 be be VB cord-008590-xivnsldf 340 42 responsible responsible JJ cord-008590-xivnsldf 340 43 . . . cord-008590-xivnsldf 341 1 In in IN cord-008590-xivnsldf 341 2 this this DT cord-008590-xivnsldf 341 3 sense sense NN cord-008590-xivnsldf 341 4 , , , cord-008590-xivnsldf 341 5 the the DT cord-008590-xivnsldf 341 6 length length NN cord-008590-xivnsldf 341 7 of of IN cord-008590-xivnsldf 341 8 an an DT cord-008590-xivnsldf 341 9 oligosaccharide oligosaccharide NN cord-008590-xivnsldf 341 10 chain chain NN cord-008590-xivnsldf 341 11 , , , cord-008590-xivnsldf 341 12 e.g. e.g. RB cord-008590-xivnsldf 341 13 , , , cord-008590-xivnsldf 341 14 of of IN cord-008590-xivnsldf 341 15 chondroitin chondroitin NN cord-008590-xivnsldf 341 16 sulfate sulfate NN cord-008590-xivnsldf 341 17 , , , cord-008590-xivnsldf 341 18 might may MD cord-008590-xivnsldf 341 19 be be VB cord-008590-xivnsldf 341 20 considered consider VBN cord-008590-xivnsldf 341 21 an an DT cord-008590-xivnsldf 341 22 indirect indirect JJ cord-008590-xivnsldf 341 23 measure measure NN cord-008590-xivnsldf 341 24 of of IN cord-008590-xivnsldf 341 25 the the DT cord-008590-xivnsldf 341 26 kinetics kinetic NNS cord-008590-xivnsldf 341 27 of of IN cord-008590-xivnsldf 341 28 transport transport NN cord-008590-xivnsldf 341 29 . . . cord-008590-xivnsldf 342 1 For for IN cord-008590-xivnsldf 342 2 the the DT cord-008590-xivnsldf 342 3 moment moment NN cord-008590-xivnsldf 342 4 , , , cord-008590-xivnsldf 342 5 knowledge knowledge NN cord-008590-xivnsldf 342 6 of of IN cord-008590-xivnsldf 342 7 the the DT cord-008590-xivnsldf 342 8 sequences sequence NNS cord-008590-xivnsldf 342 9 of of IN cord-008590-xivnsldf 342 10 posttranslational posttranslational JJ cord-008590-xivnsldf 342 11 processing processing NN cord-008590-xivnsldf 342 12 events event NNS cord-008590-xivnsldf 342 13 can can MD cord-008590-xivnsldf 342 14 explain explain VB cord-008590-xivnsldf 342 15 the the DT cord-008590-xivnsldf 342 16 final final JJ cord-008590-xivnsldf 342 17 structures structure NNS cord-008590-xivnsldf 342 18 of of IN cord-008590-xivnsldf 342 19 many many JJ cord-008590-xivnsldf 342 20 macromolecules macromolecule NNS cord-008590-xivnsldf 342 21 which which WDT cord-008590-xivnsldf 342 22 pass pass VBP cord-008590-xivnsldf 342 23 through through IN cord-008590-xivnsldf 342 24 the the DT cord-008590-xivnsldf 342 25 GC GC NNP cord-008590-xivnsldf 342 26 . . . cord-008590-xivnsldf 343 1 This this DT cord-008590-xivnsldf 343 2 article article NN cord-008590-xivnsldf 343 3 has have VBZ cord-008590-xivnsldf 343 4 summarized summarize VBN cord-008590-xivnsldf 343 5 the the DT cord-008590-xivnsldf 343 6 incomplete incomplete JJ cord-008590-xivnsldf 343 7 data datum NNS cord-008590-xivnsldf 343 8 now now RB cord-008590-xivnsldf 343 9 available available JJ cord-008590-xivnsldf 343 10 which which WDT cord-008590-xivnsldf 343 11 points point VBZ cord-008590-xivnsldf 343 12 to to IN cord-008590-xivnsldf 343 13 subcompartmentalization subcompartmentalization NN cord-008590-xivnsldf 343 14 of of IN cord-008590-xivnsldf 343 15 these these DT cord-008590-xivnsldf 343 16 events event NNS cord-008590-xivnsldf 343 17 . . . cord-008590-xivnsldf 344 1 It -PRON- PRP cord-008590-xivnsldf 344 2 is be VBZ cord-008590-xivnsldf 344 3 to to TO cord-008590-xivnsldf 344 4 be be VB cord-008590-xivnsldf 344 5 anticipated anticipate VBN cord-008590-xivnsldf 344 6 that that IN cord-008590-xivnsldf 344 7 as as IN cord-008590-xivnsldf 344 8 the the DT cord-008590-xivnsldf 344 9 assignment assignment NN cord-008590-xivnsldf 344 10 of of IN cord-008590-xivnsldf 344 11 specific specific JJ cord-008590-xivnsldf 344 12 steps step NNS cord-008590-xivnsldf 344 13 to to IN cord-008590-xivnsldf 344 14 subcompartments subcompartment NNS cord-008590-xivnsldf 344 15 is be VBZ cord-008590-xivnsldf 344 16 made make VBN cord-008590-xivnsldf 344 17 with with IN cord-008590-xivnsldf 344 18 greater great JJR cord-008590-xivnsldf 344 19 certainty certainty NN cord-008590-xivnsldf 344 20 , , , cord-008590-xivnsldf 344 21 this this DT cord-008590-xivnsldf 344 22 information information NN cord-008590-xivnsldf 344 23 will will MD cord-008590-xivnsldf 344 24 lead lead VB cord-008590-xivnsldf 344 25 to to IN cord-008590-xivnsldf 344 26 an an DT cord-008590-xivnsldf 344 27 increased increase VBN cord-008590-xivnsldf 344 28 appreciation appreciation NN cord-008590-xivnsldf 344 29 and and CC cord-008590-xivnsldf 344 30 understanding understanding NN cord-008590-xivnsldf 344 31 of of IN cord-008590-xivnsldf 344 32 the the DT cord-008590-xivnsldf 344 33 dynamics dynamic NNS cord-008590-xivnsldf 344 34 of of IN cord-008590-xivnsldf 344 35 membrane membrane NN cord-008590-xivnsldf 344 36 - - HYPH cord-008590-xivnsldf 344 37 membrane membrane NN cord-008590-xivnsldf 344 38 interactions interaction NNS cord-008590-xivnsldf 344 39 which which WDT cord-008590-xivnsldf 344 40 underly underly VBP cord-008590-xivnsldf 344 41 the the DT cord-008590-xivnsldf 344 42 operation operation NN cord-008590-xivnsldf 344 43 of of IN cord-008590-xivnsldf 344 44 the the DT cord-008590-xivnsldf 344 45 organelle organelle NN cord-008590-xivnsldf 344 46 as as IN cord-008590-xivnsldf 344 47 a a DT cord-008590-xivnsldf 344 48 whole whole NN cord-008590-xivnsldf 344 49 . . . cord-008590-xivnsldf 345 1 Plenum Plenum NNP cord-008590-xivnsldf 345 2 , , , cord-008590-xivnsldf 345 3 20 20 CD cord-008590-xivnsldf 345 4 Transport Transport NNP cord-008590-xivnsldf 345 5 of of IN cord-008590-xivnsldf 345 6 Macromolecules Macromolecules NNP cord-008590-xivnsldf 345 7 in in IN cord-008590-xivnsldf 345 8 Cellular Cellular NNP cord-008590-xivnsldf 345 9 Systems Systems NNPS cord-008590-xivnsldf 345 10 Cell Cell NNP cord-008590-xivnsldf 345 11 Surface surface NN cord-008590-xivnsldf 345 12 Carbohydrate Carbohydrate NNP cord-008590-xivnsldf 345 13 Chemistry Chemistry NNP cord-008590-xivnsldf 346 1 I -PRON- PRP cord-008590-xivnsldf 346 2 n n CC cord-008590-xivnsldf 346 3 " " `` cord-008590-xivnsldf 346 4 Structural Structural NNP cord-008590-xivnsldf 346 5 Carbohydrates carbohydrate NNS cord-008590-xivnsldf 346 6 in in IN cord-008590-xivnsldf 346 7 the the DT cord-008590-xivnsldf 346 8 Liver liver NN cord-008590-xivnsldf 346 9 Proc proc NN cord-008590-xivnsldf 346 10 . . . cord-008590-xivnsldf 347 1 Narl Narl NNP cord-008590-xivnsldf 347 2 . . . cord-008590-xivnsldf 348 1 Acad Acad NNP cord-008590-xivnsldf 348 2 . . . cord-008590-xivnsldf 349 1 Sci Sci NNP cord-008590-xivnsldf 349 2 Biochemistry Biochemistry NNP cord-008590-xivnsldf 349 3 of of IN cord-008590-xivnsldf 349 4 Glycoproteins Glycoproteins NNP cord-008590-xivnsldf 349 5 and and CC cord-008590-xivnsldf 349 6 Proteoglycans Proteoglycans NNPS cord-008590-xivnsldf 349 7 Proc Proc NNP cord-008590-xivnsldf 349 8 . . . cord-008590-xivnsldf 350 1 Nut/. Nut/. NNP cord-008590-xivnsldf 350 2 Acad Acad NNP cord-008590-xivnsldf 350 3 . . . cord-008590-xivnsldf 351 1 Sci Sci NNP cord-008590-xivnsldf 351 2 Biochemistry Biochemistry NNP cord-008590-xivnsldf 351 3 of of IN cord-008590-xivnsldf 351 4 Glycoproteins Glycoproteins NNP cord-008590-xivnsldf 351 5 and and CC cord-008590-xivnsldf 351 6 Proteoglvcans Proteoglvcans NNPS cord-008590-xivnsldf 351 7 Biochemistry Biochemistry NNP cord-008590-xivnsldf 351 8 of of IN cord-008590-xivnsldf 351 9 Glycoproteins Glycoproteins NNP cord-008590-xivnsldf 351 10 and and CC cord-008590-xivnsldf 351 11 Proteoglycans Proteoglycans NNPS cord-008590-xivnsldf 351 12 Mucus Mucus NNP cord-008590-xivnsldf 351 13 in in IN cord-008590-xivnsldf 351 14 Health Health NNP cord-008590-xivnsldf 351 15 and and CC cord-008590-xivnsldf 351 16 Disease Disease NNP cord-008590-xivnsldf 351 17 Proc Proc NNP cord-008590-xivnsldf 351 18 . . . cord-008590-xivnsldf 352 1 Int Int NNP cord-008590-xivnsldf 352 2 . . . cord-008590-xivnsldf 353 1 Svmp Svmp NNP cord-008590-xivnsldf 353 2 . . . cord-008590-xivnsldf 354 1 Gl.vcoconj Gl.vcoconj NNP cord-008590-xivnsldf 354 2 . . . cord-008590-xivnsldf 355 1 4th 4th JJ cord-008590-xivnsldf 355 2 Eiochem Eiochem NNP cord-008590-xivnsldf 355 3 Merhods Merhods NNPS cord-008590-xivnsldf 355 4 124a 124a NNPS cord-008590-xivnsldf 355 5 . . . cord-008590-xivnsldf 356 1 Smith Smith NNP cord-008590-xivnsldf 356 2 , , , cord-008590-xivnsldf 356 3 C. C. NNP cord-008590-xivnsldf 356 4 ( ( -LRB- cord-008590-xivnsldf 356 5 1981 1981 CD cord-008590-xivnsldf 356 6 ) ) -RRB- cord-008590-xivnsldf 356 7 Proc proc NN cord-008590-xivnsldf 356 8 . . . cord-008590-xivnsldf 357 1 Narl Narl NNP cord-008590-xivnsldf 357 2 . . . cord-008590-xivnsldf 358 1 Acad Acad NNP cord-008590-xivnsldf 358 2 . . . cord-008590-xivnsldf 359 1 Sci Sci NNP cord-008590-xivnsldf 359 2 . . NNP cord-008590-xivnsldf 359 3 U.S.A. U.S.A. NNP cord-008590-xivnsldf 359 4 54 54 CD cord-008590-xivnsldf 359 5 , , , cord-008590-xivnsldf 359 6 141 141 CD cord-008590-xivnsldf 359 7 . . . cord-008590-xivnsldf 360 1 Thonar Thonar NNP