id	sid	tid	token	lemma	pos
cord-007261-b5fgb9wf	1	1	key	key	JJ
cord-007261-b5fgb9wf	2	1	:	:	:
cord-007261-b5fgb9wf	2	2	cord-007261-b5fgb9wf	cord-007261-b5fgb9wf	NN
cord-007261-b5fgb9wf	2	3	authors	author	NNS
cord-007261-b5fgb9wf	2	4	:	:	:
cord-007261-b5fgb9wf	2	5	Murakami	Murakami	NNP
cord-007261-b5fgb9wf	2	6	,	,	,
cord-007261-b5fgb9wf	2	7	Kazuya	Kazuya	NNP
cord-007261-b5fgb9wf	2	8	;	;	:
cord-007261-b5fgb9wf	2	9	Mihara	Mihara	NNP
cord-007261-b5fgb9wf	2	10	,	,	,
cord-007261-b5fgb9wf	2	11	Katsuyosi	Katsuyosi	NNP
cord-007261-b5fgb9wf	2	12	;	;	:
cord-007261-b5fgb9wf	2	13	Omura	Omura	NNP
cord-007261-b5fgb9wf	2	14	,	,	,
cord-007261-b5fgb9wf	3	1	Tsuneo	Tsuneo	NNP
cord-007261-b5fgb9wf	3	2	title	title	NN
cord-007261-b5fgb9wf	3	3	:	:	:
cord-007261-b5fgb9wf	3	4	The	the	DT
cord-007261-b5fgb9wf	3	5	Transmembrane	Transmembrane	NNP
cord-007261-b5fgb9wf	3	6	Region	Region	NNP
cord-007261-b5fgb9wf	3	7	of	of	IN
cord-007261-b5fgb9wf	3	8	Microsomal	Microsomal	NNP
cord-007261-b5fgb9wf	3	9	Cytochrome	Cytochrome	NNP
cord-007261-b5fgb9wf	3	10	P450	P450	NNPS
cord-007261-b5fgb9wf	3	11	Identified	identify	VBN
cord-007261-b5fgb9wf	3	12	as	as	IN
cord-007261-b5fgb9wf	3	13	the	the	DT
cord-007261-b5fgb9wf	3	14	Endoplasmic	Endoplasmic	NNP
cord-007261-b5fgb9wf	3	15	Reticulum	Reticulum	NNP
cord-007261-b5fgb9wf	3	16	Retention	Retention	NNP
cord-007261-b5fgb9wf	3	17	Signal(1	Signal(1	NNP
cord-007261-b5fgb9wf	3	18	)	)	-RRB-
cord-007261-b5fgb9wf	3	19	date	date	NN
cord-007261-b5fgb9wf	3	20	:	:	:
cord-007261-b5fgb9wf	3	21	1994	1994	CD
cord-007261-b5fgb9wf	3	22	-	-	SYM
cord-007261-b5fgb9wf	3	23	07	07	CD
cord-007261-b5fgb9wf	3	24	-	-	SYM
cord-007261-b5fgb9wf	3	25	17	17	CD
cord-007261-b5fgb9wf	3	26	journal	journal	NN
cord-007261-b5fgb9wf	3	27	:	:	:
cord-007261-b5fgb9wf	3	28	J	J	NNP
cord-007261-b5fgb9wf	3	29	Biochem	Biochem	NNP
cord-007261-b5fgb9wf	3	30	DOI	DOI	NNP
cord-007261-b5fgb9wf	3	31	:	:	:
cord-007261-b5fgb9wf	3	32	10.1093	10.1093	CD
cord-007261-b5fgb9wf	3	33	/	/	SYM
cord-007261-b5fgb9wf	3	34	oxfordjournals.jbchem.a124489	oxfordjournals.jbchem.a124489	NN
cord-007261-b5fgb9wf	3	35	sha	sha	NNP
cord-007261-b5fgb9wf	3	36	:	:	:
cord-007261-b5fgb9wf	4	1	8be9fb2ea6f60cdc3b913295e1157ba1bafe3a64	8be9fb2ea6f60cdc3b913295e1157ba1bafe3a64	CD
cord-007261-b5fgb9wf	4	2	doc_id	doc_id	CD
cord-007261-b5fgb9wf	4	3	:	:	:
cord-007261-b5fgb9wf	4	4	7261	7261	CD
cord-007261-b5fgb9wf	4	5	cord_uid	cord_uid	NNS
cord-007261-b5fgb9wf	4	6	:	:	:
cord-007261-b5fgb9wf	4	7	b5fgb9wf	b5fgb9wf	NNP
cord-007261-b5fgb9wf	4	8	Microsomal	Microsomal	NNP
cord-007261-b5fgb9wf	4	9	-	-	HYPH
cord-007261-b5fgb9wf	4	10	type	type	NN
cord-007261-b5fgb9wf	4	11	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	5	1	P450s	P450s	NNP
cord-007261-b5fgb9wf	5	2	are	be	VBP
cord-007261-b5fgb9wf	5	3	integral	integral	JJ
cord-007261-b5fgb9wf	5	4	membrane	membrane	NN
cord-007261-b5fgb9wf	5	5	proteins	protein	NNS
cord-007261-b5fgb9wf	5	6	bound	bind	VBN
cord-007261-b5fgb9wf	5	7	to	to	IN
cord-007261-b5fgb9wf	5	8	the	the	DT
cord-007261-b5fgb9wf	5	9	membrane	membrane	NN
cord-007261-b5fgb9wf	5	10	through	through	IN
cord-007261-b5fgb9wf	5	11	their	-PRON-	PRP$
cord-007261-b5fgb9wf	5	12	N	n	CD
cord-007261-b5fgb9wf	5	13	-	-	HYPH
cord-007261-b5fgb9wf	5	14	terminal	terminal	NN
cord-007261-b5fgb9wf	5	15	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	5	16	hydrophobic	hydrophobic	JJ
cord-007261-b5fgb9wf	5	17	segment	segment	NN
cord-007261-b5fgb9wf	5	18	,	,	,
cord-007261-b5fgb9wf	5	19	the	the	DT
cord-007261-b5fgb9wf	5	20	signal	signal	NN
cord-007261-b5fgb9wf	5	21	anchor	anchor	NN
cord-007261-b5fgb9wf	5	22	sequence	sequence	NN
cord-007261-b5fgb9wf	5	23	.	.	.
cord-007261-b5fgb9wf	6	1	To	to	TO
cord-007261-b5fgb9wf	6	2	elucidate	elucidate	VB
cord-007261-b5fgb9wf	6	3	the	the	DT
cord-007261-b5fgb9wf	6	4	determinants	determinant	NNS
cord-007261-b5fgb9wf	6	5	that	that	WDT
cord-007261-b5fgb9wf	6	6	enable	enable	VBP
cord-007261-b5fgb9wf	6	7	the	the	DT
cord-007261-b5fgb9wf	6	8	P450s	P450s	NNP
cord-007261-b5fgb9wf	6	9	to	to	TO
cord-007261-b5fgb9wf	6	10	be	be	VB
cord-007261-b5fgb9wf	6	11	located	locate	VBN
cord-007261-b5fgb9wf	6	12	in	in	IN
cord-007261-b5fgb9wf	6	13	the	the	DT
cord-007261-b5fgb9wf	6	14	ER	ER	NNP
cord-007261-b5fgb9wf	6	15	,	,	,
cord-007261-b5fgb9wf	6	16	we	-PRON-	PRP
cord-007261-b5fgb9wf	6	17	constructed	construct	VBD
cord-007261-b5fgb9wf	6	18	cDNAs	cDNAs	:
cord-007261-b5fgb9wf	6	19	encoding	encode	VBG
cord-007261-b5fgb9wf	6	20	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	6	21	proteins	protein	NNS
cord-007261-b5fgb9wf	6	22	in	in	IN
cord-007261-b5fgb9wf	6	23	which	which	WDT
cord-007261-b5fgb9wf	6	24	a	a	DT
cord-007261-b5fgb9wf	6	25	secretory	secretory	JJ
cord-007261-b5fgb9wf	6	26	form	form	NN
cord-007261-b5fgb9wf	6	27	of	of	IN
cord-007261-b5fgb9wf	6	28	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	6	29	,	,	,
cord-007261-b5fgb9wf	6	30	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	6	31	Sec	Sec	NNP
cord-007261-b5fgb9wf	6	32	,	,	,
cord-007261-b5fgb9wf	6	33	was	be	VBD
cord-007261-b5fgb9wf	6	34	connected	connect	VBN
cord-007261-b5fgb9wf	6	35	to	to	IN
cord-007261-b5fgb9wf	6	36	the	the	DT
cord-007261-b5fgb9wf	6	37	N	n	NN
cord-007261-b5fgb9wf	6	38	-	-	HYPH
cord-007261-b5fgb9wf	6	39	terminus	terminus	NN
cord-007261-b5fgb9wf	6	40	of	of	IN
cord-007261-b5fgb9wf	6	41	the	the	DT
cord-007261-b5fgb9wf	6	42	full	full	JJ
cord-007261-b5fgb9wf	6	43	-	-	HYPH
cord-007261-b5fgb9wf	6	44	length	length	NN
cord-007261-b5fgb9wf	6	45	or	or	CC
cord-007261-b5fgb9wf	6	46	truncated	truncated	JJ
cord-007261-b5fgb9wf	6	47	forms	form	NNS
cord-007261-b5fgb9wf	6	48	of	of	IN
cord-007261-b5fgb9wf	6	49	a	a	DT
cord-007261-b5fgb9wf	6	50	microsomal	microsomal	JJ
cord-007261-b5fgb9wf	6	51	-	-	HYPH
cord-007261-b5fgb9wf	6	52	type	type	NN
cord-007261-b5fgb9wf	6	53	P450	p450	NN
cord-007261-b5fgb9wf	6	54	,	,	,
cord-007261-b5fgb9wf	6	55	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	6	56	)	)	-RRB-
cord-007261-b5fgb9wf	6	57	,	,	,
cord-007261-b5fgb9wf	6	58	and	and	CC
cord-007261-b5fgb9wf	6	59	the	the	DT
cord-007261-b5fgb9wf	6	60	constructed	construct	VBN
cord-007261-b5fgb9wf	6	61	plasmids	plasmid	NNS
cord-007261-b5fgb9wf	6	62	were	be	VBD
cord-007261-b5fgb9wf	6	63	expressed	express	VBN
cord-007261-b5fgb9wf	6	64	in	in	IN
cord-007261-b5fgb9wf	6	65	COS	COS	NNP
cord-007261-b5fgb9wf	6	66	cells	cell	NNS
cord-007261-b5fgb9wf	6	67	.	.	.
cord-007261-b5fgb9wf	7	1	Since	since	IN
cord-007261-b5fgb9wf	7	2	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	7	3	Sec	Sec	NNP
cord-007261-b5fgb9wf	7	4	is	be	VBZ
cord-007261-b5fgb9wf	7	5	an	an	DT
cord-007261-b5fgb9wf	7	6	iV	iv	CD
cord-007261-b5fgb9wf	7	7	-	-	HYPH
cord-007261-b5fgb9wf	7	8	glycosylated	glycosylate	VBN
cord-007261-b5fgb9wf	7	9	secretory	secretory	JJ
cord-007261-b5fgb9wf	7	10	protein	protein	NN
cord-007261-b5fgb9wf	7	11	,	,	,
cord-007261-b5fgb9wf	7	12	endo	endo	NN
cord-007261-b5fgb9wf	7	13	H	h	NN
cord-007261-b5fgb9wf	7	14	treatment	treatment	NN
cord-007261-b5fgb9wf	7	15	could	could	MD
cord-007261-b5fgb9wf	7	16	be	be	VB
cord-007261-b5fgb9wf	7	17	used	use	VBN
cord-007261-b5fgb9wf	7	18	to	to	TO
cord-007261-b5fgb9wf	7	19	determine	determine	VB
cord-007261-b5fgb9wf	7	20	whether	whether	IN
cord-007261-b5fgb9wf	7	21	these	these	DT
cord-007261-b5fgb9wf	7	22	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	7	23	proteins	protein	NNS
cord-007261-b5fgb9wf	7	24	were	be	VBD
cord-007261-b5fgb9wf	7	25	located	locate	VBN
cord-007261-b5fgb9wf	7	26	in	in	IN
cord-007261-b5fgb9wf	7	27	the	the	DT
cord-007261-b5fgb9wf	7	28	ER	er	NN
cord-007261-b5fgb9wf	7	29	or	or	CC
cord-007261-b5fgb9wf	7	30	not	not	RB
cord-007261-b5fgb9wf	7	31	.	.	.
cord-007261-b5fgb9wf	8	1	Carboxyesterase	Carboxyesterase	NNP
cord-007261-b5fgb9wf	8	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	8	3	with	with	IN
cord-007261-b5fgb9wf	8	4	the	the	DT
cord-007261-b5fgb9wf	8	5	N	n	CD
cord-007261-b5fgb9wf	8	6	-	-	HYPH
cord-007261-b5fgb9wf	8	7	terminal	terminal	JJ
cord-007261-b5fgb9wf	8	8	20	20	CD
cord-007261-b5fgb9wf	8	9	amino	amino	NN
cord-007261-b5fgb9wf	8	10	acids	acid	NNS
cord-007261-b5fgb9wf	8	11	,	,	,
cord-007261-b5fgb9wf	8	12	containing	contain	VBG
cord-007261-b5fgb9wf	8	13	the	the	DT
cord-007261-b5fgb9wf	8	14	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	8	15	region	region	NN
cord-007261-b5fgb9wf	8	16	,	,	,
cord-007261-b5fgb9wf	8	17	of	of	IN
cord-007261-b5fgb9wf	8	18	P46O(M1	p46o(m1	NN
cord-007261-b5fgb9wf	8	19	)	)	-RRB-
cord-007261-b5fgb9wf	8	20	,	,	,
cord-007261-b5fgb9wf	8	21	was	be	VBD
cord-007261-b5fgb9wf	8	22	located	locate	VBN
cord-007261-b5fgb9wf	8	23	in	in	IN
cord-007261-b5fgb9wf	8	24	the	the	DT
cord-007261-b5fgb9wf	8	25	ER	ER	NNP
cord-007261-b5fgb9wf	8	26	,	,	,
cord-007261-b5fgb9wf	8	27	as	as	IN
cord-007261-b5fgb9wf	8	28	determined	determine	VBN
cord-007261-b5fgb9wf	8	29	from	from	IN
cord-007261-b5fgb9wf	8	30	the	the	DT
cord-007261-b5fgb9wf	8	31	endo	endo	NN
cord-007261-b5fgb9wf	8	32	H	h	NN
cord-007261-b5fgb9wf	8	33	sensitivity	sensitivity	NN
cord-007261-b5fgb9wf	8	34	of	of	IN
cord-007261-b5fgb9wf	8	35	the	the	DT
cord-007261-b5fgb9wf	8	36	expressed	express	VBN
cord-007261-b5fgb9wf	8	37	protein	protein	NN
cord-007261-b5fgb9wf	8	38	and	and	CC
cord-007261-b5fgb9wf	8	39	immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	8	40	staining	staining	NN
cord-007261-b5fgb9wf	8	41	of	of	IN
cord-007261-b5fgb9wf	8	42	the	the	DT
cord-007261-b5fgb9wf	8	43	cells	cell	NNS
cord-007261-b5fgb9wf	8	44	.	.	.
cord-007261-b5fgb9wf	9	1	As	as	IN
cord-007261-b5fgb9wf	9	2	the	the	DT
cord-007261-b5fgb9wf	9	3	expressed	express	VBN
cord-007261-b5fgb9wf	9	4	protein	protein	NN
cord-007261-b5fgb9wf	9	5	exhibited	exhibit	VBD
cord-007261-b5fgb9wf	9	6	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	9	7	activity	activity	NN
cord-007261-b5fgb9wf	9	8	,	,	,
cord-007261-b5fgb9wf	9	9	it	-PRON-	PRP
cord-007261-b5fgb9wf	9	10	was	be	VBD
cord-007261-b5fgb9wf	9	11	not	not	RB
cord-007261-b5fgb9wf	9	12	retained	retain	VBN
cord-007261-b5fgb9wf	9	13	in	in	IN
cord-007261-b5fgb9wf	9	14	the	the	DT
cord-007261-b5fgb9wf	9	15	ER	ER	NNP
cord-007261-b5fgb9wf	9	16	through	through	IN
cord-007261-b5fgb9wf	9	17	the	the	DT
cord-007261-b5fgb9wf	9	18	BiP	bip	NN
cord-007261-b5fgb9wf	9	19	-	-	HYPH
cord-007261-b5fgb9wf	9	20	dependent	dependent	JJ
cord-007261-b5fgb9wf	9	21	quality	quality	NN
cord-007261-b5fgb9wf	9	22	control	control	NN
cord-007261-b5fgb9wf	9	23	system	system	NN
cord-007261-b5fgb9wf	9	24	recognizing	recognize	VBG
cord-007261-b5fgb9wf	9	25	unfolded	unfold	VBN
cord-007261-b5fgb9wf	9	26	proteins	protein	NNS
cord-007261-b5fgb9wf	9	27	.	.	.
cord-007261-b5fgb9wf	10	1	Another	another	DT
cord-007261-b5fgb9wf	10	2	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	10	3	protein	protein	NN
cord-007261-b5fgb9wf	10	4	construct	construct	NN
cord-007261-b5fgb9wf	10	5	in	in	IN
cord-007261-b5fgb9wf	10	6	which	which	WDT
cord-007261-b5fgb9wf	10	7	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	10	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	10	9	was	be	VBD
cord-007261-b5fgb9wf	10	10	connected	connect	VBN
cord-007261-b5fgb9wf	10	11	to	to	IN
cord-007261-b5fgb9wf	10	12	the	the	DT
cord-007261-b5fgb9wf	10	13	C	C	NNP
cord-007261-b5fgb9wf	10	14	-	-	HYPH
cord-007261-b5fgb9wf	10	15	terminal	terminal	NN
cord-007261-b5fgb9wf	10	16	region	region	NN
cord-007261-b5fgb9wf	10	17	of	of	IN
cord-007261-b5fgb9wf	10	18	rat	rat	NN
cord-007261-b5fgb9wf	10	19	UDP	UDP	NNP
cord-007261-b5fgb9wf	10	20	-	-	HYPH
cord-007261-b5fgb9wf	10	21	glucuronosyltransferase	glucuronosyltransferase	NNP
cord-007261-b5fgb9wf	11	1	(	(	-LRB-
cord-007261-b5fgb9wf	11	2	UDP	UDP	NNP
cord-007261-b5fgb9wf	11	3	-	-	HYPH
cord-007261-b5fgb9wf	11	4	GT	GT	NNP
cord-007261-b5fgb9wf	11	5	)	)	-RRB-
cord-007261-b5fgb9wf	12	1	,	,	,
cord-007261-b5fgb9wf	12	2	that	that	DT
cord-007261-b5fgb9wf	12	3	contained	contain	VBD
cord-007261-b5fgb9wf	12	4	a	a	DT
cord-007261-b5fgb9wf	12	5	double	double	JJ
cord-007261-b5fgb9wf	12	6	-	-	HYPH
cord-007261-b5fgb9wf	12	7	lysin	lysin	NN
cord-007261-b5fgb9wf	12	8	ER	er	NN
cord-007261-b5fgb9wf	12	9	retention	retention	NN
cord-007261-b5fgb9wf	12	10	motif	motif	NN
cord-007261-b5fgb9wf	12	11	,	,	,
cord-007261-b5fgb9wf	12	12	was	be	VBD
cord-007261-b5fgb9wf	12	13	also	also	RB
cord-007261-b5fgb9wf	12	14	located	locate	VBN
cord-007261-b5fgb9wf	12	15	in	in	IN
cord-007261-b5fgb9wf	12	16	the	the	DT
cord-007261-b5fgb9wf	12	17	ER	ER	NNP
cord-007261-b5fgb9wf	12	18	,	,	,
cord-007261-b5fgb9wf	12	19	as	as	IN
cord-007261-b5fgb9wf	12	20	determined	determine	VBN
cord-007261-b5fgb9wf	12	21	from	from	IN
cord-007261-b5fgb9wf	12	22	the	the	DT
cord-007261-b5fgb9wf	12	23	endo	endo	NN
cord-007261-b5fgb9wf	12	24	H	h	NN
cord-007261-b5fgb9wf	12	25	sensitivity	sensitivity	NN
cord-007261-b5fgb9wf	12	26	and	and	CC
cord-007261-b5fgb9wf	12	27	immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	12	28	staining	staining	NN
cord-007261-b5fgb9wf	12	29	.	.	.
cord-007261-b5fgb9wf	13	1	On	on	IN
cord-007261-b5fgb9wf	13	2	the	the	DT
cord-007261-b5fgb9wf	13	3	other	other	JJ
cord-007261-b5fgb9wf	13	4	hand	hand	NN
cord-007261-b5fgb9wf	13	5	,	,	,
cord-007261-b5fgb9wf	13	6	the	the	DT
cord-007261-b5fgb9wf	13	7	sugar	sugar	NN
cord-007261-b5fgb9wf	13	8	moiety	moiety	NN
cord-007261-b5fgb9wf	13	9	of	of	IN
cord-007261-b5fgb9wf	13	10	the	the	DT
cord-007261-b5fgb9wf	13	11	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	14	1	Sec	Sec	NNP
cord-007261-b5fgb9wf	14	2	connected	connect	VBN
cord-007261-b5fgb9wf	14	3	to	to	IN
cord-007261-b5fgb9wf	14	4	the	the	DT
cord-007261-b5fgb9wf	14	5	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	14	6	segment	segment	NN
cord-007261-b5fgb9wf	14	7	of	of	IN
cord-007261-b5fgb9wf	14	8	UDP	UDP	NNP
cord-007261-b5fgb9wf	14	9	-	-	HYPH
cord-007261-b5fgb9wf	14	10	GT	GT	NNP
cord-007261-b5fgb9wf	14	11	,	,	,
cord-007261-b5fgb9wf	14	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	14	13	/	/	SYM
cord-007261-b5fgb9wf	14	14	GTd	GTd	NNS
cord-007261-b5fgb9wf	14	15	,	,	,
cord-007261-b5fgb9wf	14	16	was	be	VBD
cord-007261-b5fgb9wf	14	17	partially	partially	RB
cord-007261-b5fgb9wf	14	18	resistant	resistant	JJ
cord-007261-b5fgb9wf	14	19	to	to	IN
cord-007261-b5fgb9wf	14	20	the	the	DT
cord-007261-b5fgb9wf	14	21	endo	endo	NN
cord-007261-b5fgb9wf	14	22	H	H	NNP
cord-007261-b5fgb9wf	14	23	treatment	treatment	NN
cord-007261-b5fgb9wf	14	24	.	.	.
cord-007261-b5fgb9wf	15	1	From	from	IN
cord-007261-b5fgb9wf	15	2	the	the	DT
cord-007261-b5fgb9wf	15	3	results	result	NNS
cord-007261-b5fgb9wf	15	4	of	of	IN
cord-007261-b5fgb9wf	15	5	immunofluorescent	immunofluorescent	JJ
cord-007261-b5fgb9wf	15	6	staining	staining	NN
cord-007261-b5fgb9wf	15	7	and	and	CC
cord-007261-b5fgb9wf	15	8	cell	cell	NN
cord-007261-b5fgb9wf	15	9	fractiona	fractiona	NN
cord-007261-b5fgb9wf	15	10	-	-	HYPH
cord-007261-b5fgb9wf	15	11	tion	tion	NN
cord-007261-b5fgb9wf	15	12	,	,	,
cord-007261-b5fgb9wf	15	13	it	-PRON-	PRP
cord-007261-b5fgb9wf	15	14	was	be	VBD
cord-007261-b5fgb9wf	15	15	concluded	conclude	VBN
cord-007261-b5fgb9wf	15	16	that	that	IN
cord-007261-b5fgb9wf	15	17	the	the	DT
cord-007261-b5fgb9wf	15	18	Sec	Sec	NNP
cord-007261-b5fgb9wf	15	19	/	/	SYM
cord-007261-b5fgb9wf	15	20	GTd	gtd	NN
cord-007261-b5fgb9wf	15	21	product	product	NN
cord-007261-b5fgb9wf	15	22	was	be	VBD
cord-007261-b5fgb9wf	15	23	located	locate	VBN
cord-007261-b5fgb9wf	15	24	in	in	IN
cord-007261-b5fgb9wf	15	25	the	the	DT
cord-007261-b5fgb9wf	15	26	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	15	27	apparatus	apparatus	NN
cord-007261-b5fgb9wf	15	28	.	.	.
cord-007261-b5fgb9wf	16	1	These	these	DT
cord-007261-b5fgb9wf	16	2	observations	observation	NNS
cord-007261-b5fgb9wf	16	3	indicated	indicate	VBD
cord-007261-b5fgb9wf	16	4	that	that	IN
cord-007261-b5fgb9wf	16	5	the	the	DT
cord-007261-b5fgb9wf	16	6	N	n	CD
cord-007261-b5fgb9wf	16	7	-	-	HYPH
cord-007261-b5fgb9wf	16	8	terminal	terminal	JJ
cord-007261-b5fgb9wf	16	9	hydrophobic	hydrophobic	JJ
cord-007261-b5fgb9wf	16	10	segment	segment	NN
cord-007261-b5fgb9wf	16	11	of	of	IN
cord-007261-b5fgb9wf	16	12	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	16	13	)	)	-RRB-
cord-007261-b5fgb9wf	16	14	is	be	VBZ
cord-007261-b5fgb9wf	16	15	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	16	16	for	for	IN
cord-007261-b5fgb9wf	16	17	the	the	DT
cord-007261-b5fgb9wf	16	18	ER	ER	NNP
cord-007261-b5fgb9wf	16	19	membrane	membrane	NN
cord-007261-b5fgb9wf	16	20	retention	retention	NN
cord-007261-b5fgb9wf	16	21	,	,	,
cord-007261-b5fgb9wf	16	22	whereas	whereas	IN
cord-007261-b5fgb9wf	16	23	the	the	DT
cord-007261-b5fgb9wf	16	24	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	16	25	segment	segment	NN
cord-007261-b5fgb9wf	16	26	of	of	IN
cord-007261-b5fgb9wf	16	27	UDP	UDP	NNP
cord-007261-b5fgb9wf	16	28	-	-	HYPH
cord-007261-b5fgb9wf	16	29	GT	GT	NNP
cord-007261-b5fgb9wf	16	30	is	be	VBZ
cord-007261-b5fgb9wf	16	31	not	not	RB
cord-007261-b5fgb9wf	16	32	.	.	.
cord-007261-b5fgb9wf	17	1	To	to	TO
cord-007261-b5fgb9wf	17	2	determine	determine	VB
cord-007261-b5fgb9wf	17	3	whether	whether	IN
cord-007261-b5fgb9wf	17	4	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	17	5	P450s	P450s	NNP
cord-007261-b5fgb9wf	17	6	are	be	VBP
cord-007261-b5fgb9wf	17	7	recycled	recycle	VBN
cord-007261-b5fgb9wf	17	8	between	between	IN
cord-007261-b5fgb9wf	17	9	the	the	DT
cord-007261-b5fgb9wf	17	10	ER	ER	NNP
cord-007261-b5fgb9wf	17	11	and	and	CC
cord-007261-b5fgb9wf	17	12	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	17	13	compartments	compartment	NNS
cord-007261-b5fgb9wf	17	14	or	or	CC
cord-007261-b5fgb9wf	17	15	not	not	RB
cord-007261-b5fgb9wf	17	16	,	,	,
cord-007261-b5fgb9wf	17	17	a	a	DT
cord-007261-b5fgb9wf	17	18	DNA	dna	NN
cord-007261-b5fgb9wf	17	19	construct	construct	NN
cord-007261-b5fgb9wf	17	20	encoding	encode	VBG
cord-007261-b5fgb9wf	17	21	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	17	22	D	d	NN
cord-007261-b5fgb9wf	17	23	connected	connect	VBN
cord-007261-b5fgb9wf	17	24	to	to	IN
cord-007261-b5fgb9wf	17	25	the	the	DT
cord-007261-b5fgb9wf	17	26	N	n	NN
cord-007261-b5fgb9wf	17	27	-	-	HYPH
cord-007261-b5fgb9wf	17	28	terminus	terminus	NN
cord-007261-b5fgb9wf	17	29	of	of	IN
cord-007261-b5fgb9wf	17	30	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	17	31	)	)	-RRB-
cord-007261-b5fgb9wf	17	32	was	be	VBD
cord-007261-b5fgb9wf	17	33	prepared	prepare	VBN
cord-007261-b5fgb9wf	17	34	and	and	CC
cord-007261-b5fgb9wf	17	35	expressed	express	VBN
cord-007261-b5fgb9wf	17	36	in	in	IN
cord-007261-b5fgb9wf	17	37	COS	COS	NNP
cord-007261-b5fgb9wf	17	38	cells	cell	NNS
cord-007261-b5fgb9wf	17	39	.	.	.
cord-007261-b5fgb9wf	18	1	The	the	DT
cord-007261-b5fgb9wf	18	2	fusion	fusion	NN
cord-007261-b5fgb9wf	18	3	protein	protein	NN
cord-007261-b5fgb9wf	18	4	was	be	VBD
cord-007261-b5fgb9wf	18	5	phosphorylated	phosphorylate	VBN
cord-007261-b5fgb9wf	18	6	,	,	,
cord-007261-b5fgb9wf	18	7	but	but	CC
cord-007261-b5fgb9wf	18	8	the	the	DT
cord-007261-b5fgb9wf	18	9	phosphorylation	phosphorylation	NN
cord-007261-b5fgb9wf	18	10	was	be	VBD
cord-007261-b5fgb9wf	18	11	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	18	12	to	to	IN
cord-007261-b5fgb9wf	18	13	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	18	14	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	18	15	.	.	.
cord-007261-b5fgb9wf	19	1	As	as	IN
cord-007261-b5fgb9wf	19	2	a	a	DT
cord-007261-b5fgb9wf	19	3	control	control	NN
cord-007261-b5fgb9wf	19	4	,	,	,
cord-007261-b5fgb9wf	19	5	authentic	authentic	JJ
cord-007261-b5fgb9wf	19	6	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	19	7	D	d	NN
cord-007261-b5fgb9wf	19	8	was	be	VBD
cord-007261-b5fgb9wf	19	9	subjected	subject	VBN
cord-007261-b5fgb9wf	19	10	to	to	IN
cord-007261-b5fgb9wf	19	11	phosphorylation	phosphorylation	NN
cord-007261-b5fgb9wf	19	12	of	of	IN
cord-007261-b5fgb9wf	19	13	its	-PRON-	PRP$
cord-007261-b5fgb9wf	19	14	oligosaccharide	oligosaccharide	NN
cord-007261-b5fgb9wf	19	15	chain	chain	NN
cord-007261-b5fgb9wf	19	16	that	that	WDT
cord-007261-b5fgb9wf	19	17	was	be	VBD
cord-007261-b5fgb9wf	19	18	resistant	resistant	JJ
cord-007261-b5fgb9wf	19	19	to	to	IN
cord-007261-b5fgb9wf	19	20	the	the	DT
cord-007261-b5fgb9wf	19	21	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	19	22	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	19	23	treatment	treatment	NN
cord-007261-b5fgb9wf	19	24	.	.	.
cord-007261-b5fgb9wf	20	1	Since	since	IN
cord-007261-b5fgb9wf	20	2	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	20	3	-	-	HYPH
cord-007261-b5fgb9wf	20	4	P	p	NN
cord-007261-b5fgb9wf	20	5	-	-	HYPH
cord-007261-b5fgb9wf	20	6	transferase	transferase	NN
cord-007261-b5fgb9wf	20	7	,	,	,
cord-007261-b5fgb9wf	20	8	which	which	WDT
cord-007261-b5fgb9wf	20	9	forms	form	VBZ
cord-007261-b5fgb9wf	20	10	the	the	DT
cord-007261-b5fgb9wf	20	11	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	20	12	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	20	13	-	-	HYPH
cord-007261-b5fgb9wf	20	14	resistant	resistant	JJ
cord-007261-b5fgb9wf	20	15	phosphodiester	phosphodiester	NN
cord-007261-b5fgb9wf	20	16	in	in	IN
cord-007261-b5fgb9wf	20	17	the	the	DT
cord-007261-b5fgb9wf	20	18	sugar	sugar	NN
cord-007261-b5fgb9wf	20	19	chains	chain	NNS
cord-007261-b5fgb9wf	20	20	of	of	IN
cord-007261-b5fgb9wf	20	21	lysosome	lysosome	NN
cord-007261-b5fgb9wf	20	22	-	-	HYPH
cord-007261-b5fgb9wf	20	23	targeting	target	VBG
cord-007261-b5fgb9wf	20	24	proteins	protein	NNS
cord-007261-b5fgb9wf	20	25	,	,	,
cord-007261-b5fgb9wf	20	26	is	be	VBZ
cord-007261-b5fgb9wf	20	27	located	locate	VBN
cord-007261-b5fgb9wf	20	28	in	in	IN
cord-007261-b5fgb9wf	20	29	the	the	DT
cord-007261-b5fgb9wf	20	30	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	20	31	apparatus	apparatus	NN
cord-007261-b5fgb9wf	20	32	,	,	,
cord-007261-b5fgb9wf	20	33	it	-PRON-	PRP
cord-007261-b5fgb9wf	20	34	was	be	VBD
cord-007261-b5fgb9wf	20	35	concluded	conclude	VBN
cord-007261-b5fgb9wf	20	36	that	that	IN
cord-007261-b5fgb9wf	20	37	the	the	DT
cord-007261-b5fgb9wf	20	38	oligosaccharide	oligosaccharide	NN
cord-007261-b5fgb9wf	20	39	chain	chain	NN
cord-007261-b5fgb9wf	20	40	of	of	IN
cord-007261-b5fgb9wf	20	41	the	the	DT
cord-007261-b5fgb9wf	20	42	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	20	43	D	d	NN
cord-007261-b5fgb9wf	20	44	portion	portion	NN
cord-007261-b5fgb9wf	20	45	of	of	IN
cord-007261-b5fgb9wf	20	46	the	the	DT
cord-007261-b5fgb9wf	20	47	fusion	fusion	NN
cord-007261-b5fgb9wf	20	48	protein	protein	NN
cord-007261-b5fgb9wf	20	49	was	be	VBD
cord-007261-b5fgb9wf	20	50	not	not	RB
cord-007261-b5fgb9wf	20	51	phosphorylated	phosphorylate	VBN
cord-007261-b5fgb9wf	20	52	,	,	,
cord-007261-b5fgb9wf	20	53	and	and	CC
cord-007261-b5fgb9wf	20	54	that	that	IN
cord-007261-b5fgb9wf	20	55	the	the	DT
cord-007261-b5fgb9wf	20	56	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	20	57	protein	protein	NN
cord-007261-b5fgb9wf	20	58	did	do	VBD
cord-007261-b5fgb9wf	20	59	not	not	RB
cord-007261-b5fgb9wf	20	60	go	go	VB
cord-007261-b5fgb9wf	20	61	to	to	IN
cord-007261-b5fgb9wf	20	62	the	the	DT
cord-007261-b5fgb9wf	20	63	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	20	64	apparatus	apparatus	NN
cord-007261-b5fgb9wf	20	65	.	.	.
cord-007261-b5fgb9wf	21	1	These	these	DT
cord-007261-b5fgb9wf	21	2	results	result	NNS
cord-007261-b5fgb9wf	21	3	indicate	indicate	VBP
cord-007261-b5fgb9wf	21	4	that	that	IN
cord-007261-b5fgb9wf	21	5	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	21	6	)	)	-RRB-
cord-007261-b5fgb9wf	21	7	is	be	VBZ
cord-007261-b5fgb9wf	21	8	not	not	RB
cord-007261-b5fgb9wf	21	9	recycled	recycle	VBN
cord-007261-b5fgb9wf	21	10	from	from	IN
cord-007261-b5fgb9wf	21	11	the	the	DT
cord-007261-b5fgb9wf	21	12	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	21	13	compartments	compartment	NNS
cord-007261-b5fgb9wf	21	14	to	to	IN
cord-007261-b5fgb9wf	21	15	the	the	DT
cord-007261-b5fgb9wf	21	16	ER	ER	NNP
cord-007261-b5fgb9wf	21	17	in	in	IN
cord-007261-b5fgb9wf	21	18	cells	cell	NNS
cord-007261-b5fgb9wf	21	19	.	.	.
cord-007261-b5fgb9wf	22	1	membrane	membrane	NN
cord-007261-b5fgb9wf	22	2	proteins	protein	NNS
cord-007261-b5fgb9wf	22	3	of	of	IN
cord-007261-b5fgb9wf	22	4	the	the	DT
cord-007261-b5fgb9wf	22	5	ER	ER	NNP
cord-007261-b5fgb9wf	22	6	depends	depend	VBZ
cord-007261-b5fgb9wf	22	7	on	on	IN
cord-007261-b5fgb9wf	22	8	the	the	DT
cord-007261-b5fgb9wf	22	9	presence	presence	NN
cord-007261-b5fgb9wf	22	10	of	of	IN
cord-007261-b5fgb9wf	22	11	the	the	DT
cord-007261-b5fgb9wf	22	12	C	C	NNP
cord-007261-b5fgb9wf	22	13	-	-	HYPH
cord-007261-b5fgb9wf	22	14	terminal	terminal	NN
cord-007261-b5fgb9wf	22	15	KDEL	KDEL	NNP
cord-007261-b5fgb9wf	22	16	sequence	sequence	NN
cord-007261-b5fgb9wf	22	17	.	.	.
cord-007261-b5fgb9wf	23	1	These	these	DT
cord-007261-b5fgb9wf	23	2	proteins	protein	NNS
cord-007261-b5fgb9wf	23	3	exit	exit	VBP
cord-007261-b5fgb9wf	23	4	the	the	DT
cord-007261-b5fgb9wf	23	5	ER	er	NN
cord-007261-b5fgb9wf	24	1	but	but	CC
cord-007261-b5fgb9wf	24	2	they	-PRON-	PRP
cord-007261-b5fgb9wf	24	3	are	be	VBP
cord-007261-b5fgb9wf	24	4	captured	capture	VBN
cord-007261-b5fgb9wf	24	5	by	by	IN
cord-007261-b5fgb9wf	24	6	the	the	DT
cord-007261-b5fgb9wf	24	7	KDEL	KDEL	NNP
cord-007261-b5fgb9wf	24	8	receptors	receptor	NNS
cord-007261-b5fgb9wf	24	9	in	in	IN
cord-007261-b5fgb9wf	24	10	the	the	DT
cord-007261-b5fgb9wf	24	11	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	24	12	apparatus	apparatus	NN
cord-007261-b5fgb9wf	24	13	or	or	CC
cord-007261-b5fgb9wf	24	14	in	in	IN
cord-007261-b5fgb9wf	24	15	the	the	DT
cord-007261-b5fgb9wf	24	16	ER	ER	NNP
cord-007261-b5fgb9wf	24	17	-	-	HYPH
cord-007261-b5fgb9wf	24	18	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	24	19	intermediate	intermediate	JJ
cord-007261-b5fgb9wf	24	20	compartment	compartment	NN
cord-007261-b5fgb9wf	24	21	(	(	-LRB-
cord-007261-b5fgb9wf	24	22	5	5	CD
cord-007261-b5fgb9wf	24	23	,	,	,
cord-007261-b5fgb9wf	24	24	6	6	CD
cord-007261-b5fgb9wf	24	25	)	)	-RRB-
cord-007261-b5fgb9wf	24	26	.	.	.
cord-007261-b5fgb9wf	25	1	Much	much	RB
cord-007261-b5fgb9wf	25	2	less	less	JJR
cord-007261-b5fgb9wf	25	3	is	be	VBZ
cord-007261-b5fgb9wf	25	4	known	know	VBN
cord-007261-b5fgb9wf	25	5	,	,	,
cord-007261-b5fgb9wf	25	6	however	however	RB
cord-007261-b5fgb9wf	25	7	,	,	,
cord-007261-b5fgb9wf	25	8	about	about	IN
cord-007261-b5fgb9wf	25	9	the	the	DT
cord-007261-b5fgb9wf	25	10	retention	retention	NN
cord-007261-b5fgb9wf	25	11	mechanism	mechanism	NN
cord-007261-b5fgb9wf	25	12	for	for	IN
cord-007261-b5fgb9wf	25	13	the	the	DT
cord-007261-b5fgb9wf	25	14	other	other	JJ
cord-007261-b5fgb9wf	25	15	ER	ER	NNP
cord-007261-b5fgb9wf	25	16	membrane	membrane	NN
cord-007261-b5fgb9wf	25	17	proteins	protein	NNS
cord-007261-b5fgb9wf	25	18	.	.	.
cord-007261-b5fgb9wf	26	1	The	the	DT
cord-007261-b5fgb9wf	26	2	ER	ER	NNP
cord-007261-b5fgb9wf	26	3	residency	residency	NN
cord-007261-b5fgb9wf	26	4	of	of	IN
cord-007261-b5fgb9wf	26	5	a	a	DT
cord-007261-b5fgb9wf	26	6	few	few	JJ
cord-007261-b5fgb9wf	26	7	membrane	membrane	NN
cord-007261-b5fgb9wf	26	8	proteins	protein	NNS
cord-007261-b5fgb9wf	26	9	with	with	IN
cord-007261-b5fgb9wf	26	10	the	the	DT
cord-007261-b5fgb9wf	26	11	type	type	NN
cord-007261-b5fgb9wf	26	12	I	-PRON-	PRP
cord-007261-b5fgb9wf	26	13	orientation	orientation	NN
cord-007261-b5fgb9wf	26	14	,	,	,
cord-007261-b5fgb9wf	26	15	such	such	JJ
cord-007261-b5fgb9wf	26	16	as	as	IN
cord-007261-b5fgb9wf	26	17	UDP	udp	NN
cord-007261-b5fgb9wf	26	18	-	-	HYPH
cord-007261-b5fgb9wf	26	19	glucronosyltransferase	glucronosyltransferase	NNP
cord-007261-b5fgb9wf	26	20	,	,	,
cord-007261-b5fgb9wf	26	21	is	be	VBZ
cord-007261-b5fgb9wf	26	22	guaranteed	guarantee	VBN
cord-007261-b5fgb9wf	26	23	by	by	IN
cord-007261-b5fgb9wf	26	24	the	the	DT
cord-007261-b5fgb9wf	26	25	presence	presence	NN
cord-007261-b5fgb9wf	26	26	of	of	IN
cord-007261-b5fgb9wf	26	27	the	the	DT
cord-007261-b5fgb9wf	26	28	C	C	NNP
cord-007261-b5fgb9wf	26	29	-	-	HYPH
cord-007261-b5fgb9wf	26	30	terminal	terminal	NN
cord-007261-b5fgb9wf	26	31	KKXX	KKXX	NNP
cord-007261-b5fgb9wf	26	32	or	or	CC
cord-007261-b5fgb9wf	26	33	KXKXX	KXKXX	NNP
cord-007261-b5fgb9wf	26	34	motif	motif	NN
cord-007261-b5fgb9wf	26	35	,	,	,
cord-007261-b5fgb9wf	26	36	the	the	DT
cord-007261-b5fgb9wf	26	37	double	double	JJ
cord-007261-b5fgb9wf	26	38	lysine	lysine	NN
cord-007261-b5fgb9wf	26	39	motif	motif	NN
cord-007261-b5fgb9wf	26	40	,	,	,
cord-007261-b5fgb9wf	26	41	in	in	IN
cord-007261-b5fgb9wf	26	42	their	-PRON-	PRP$
cord-007261-b5fgb9wf	26	43	short	short	JJ
cord-007261-b5fgb9wf	26	44	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	26	45	tail	tail	NN
cord-007261-b5fgb9wf	26	46	(	(	-LRB-
cord-007261-b5fgb9wf	26	47	7	7	CD
cord-007261-b5fgb9wf	26	48	)	)	-RRB-
cord-007261-b5fgb9wf	26	49	.	.	.
cord-007261-b5fgb9wf	27	1	They	-PRON-	PRP
cord-007261-b5fgb9wf	27	2	exit	exit	VBP
cord-007261-b5fgb9wf	27	3	the	the	DT
cord-007261-b5fgb9wf	27	4	ER	ER	NNP
cord-007261-b5fgb9wf	27	5	to	to	TO
cord-007261-b5fgb9wf	27	6	be	be	VB
cord-007261-b5fgb9wf	27	7	retrieved	retrieve	VBN
cord-007261-b5fgb9wf	27	8	from	from	IN
cord-007261-b5fgb9wf	27	9	the	the	DT
cord-007261-b5fgb9wf	27	10	subsequent	subsequent	JJ
cord-007261-b5fgb9wf	27	11	compartments	compartment	NNS
cord-007261-b5fgb9wf	27	12	probably	probably	RB
cord-007261-b5fgb9wf	27	13	through	through	IN
cord-007261-b5fgb9wf	27	14	a	a	DT
cord-007261-b5fgb9wf	27	15	receptormediated	receptormediate	VBN
cord-007261-b5fgb9wf	27	16	retrieval	retrieval	NN
cord-007261-b5fgb9wf	27	17	process	process	NN
cord-007261-b5fgb9wf	27	18	(	(	-LRB-
cord-007261-b5fgb9wf	27	19	8)	8)	NNP
cord-007261-b5fgb9wf	27	20	.	.	.
cord-007261-b5fgb9wf	28	1	However	however	RB
cord-007261-b5fgb9wf	28	2	,	,	,
cord-007261-b5fgb9wf	28	3	a	a	DT
cord-007261-b5fgb9wf	28	4	number	number	NN
cord-007261-b5fgb9wf	28	5	of	of	IN
cord-007261-b5fgb9wf	28	6	type	type	NN
cord-007261-b5fgb9wf	28	7	I	-PRON-	PRP
cord-007261-b5fgb9wf	28	8	ER	ER	NNP
cord-007261-b5fgb9wf	28	9	membrane	membrane	NN
cord-007261-b5fgb9wf	28	10	proteins	protein	NNS
cord-007261-b5fgb9wf	28	11	have	have	VBP
cord-007261-b5fgb9wf	28	12	no	no	DT
cord-007261-b5fgb9wf	28	13	double	double	JJ
cord-007261-b5fgb9wf	28	14	-	-	HYPH
cord-007261-b5fgb9wf	28	15	lysine	lysine	NN
cord-007261-b5fgb9wf	28	16	motif	motif	NN
cord-007261-b5fgb9wf	28	17	in	in	IN
cord-007261-b5fgb9wf	28	18	their	-PRON-	PRP$
cord-007261-b5fgb9wf	28	19	C	c	NN
cord-007261-b5fgb9wf	28	20	-	-	HYPH
cord-007261-b5fgb9wf	28	21	terminus	terminus	NN
cord-007261-b5fgb9wf	28	22	.	.	.
cord-007261-b5fgb9wf	29	1	The	the	DT
cord-007261-b5fgb9wf	29	2	retention	retention	NN
cord-007261-b5fgb9wf	29	3	mechanism	mechanism	NN
cord-007261-b5fgb9wf	29	4	for	for	IN
cord-007261-b5fgb9wf	29	5	these	these	DT
cord-007261-b5fgb9wf	29	6	proteins	protein	NNS
cord-007261-b5fgb9wf	29	7	and	and	CC
cord-007261-b5fgb9wf	29	8	the	the	DT
cord-007261-b5fgb9wf	29	9	proteins	protein	NNS
cord-007261-b5fgb9wf	29	10	with	with	IN
cord-007261-b5fgb9wf	29	11	the	the	DT
cord-007261-b5fgb9wf	29	12	type	type	NN
cord-007261-b5fgb9wf	29	13	H	h	NN
cord-007261-b5fgb9wf	29	14	orientation	orientation	NN
cord-007261-b5fgb9wf	29	15	or	or	CC
cord-007261-b5fgb9wf	29	16	with	with	IN
cord-007261-b5fgb9wf	29	17	multiple	multiple	JJ
cord-007261-b5fgb9wf	29	18	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	29	19	segments	segment	NNS
cord-007261-b5fgb9wf	29	20	remain	remain	VBP
cord-007261-b5fgb9wf	29	21	largely	largely	RB
cord-007261-b5fgb9wf	29	22	unknown	unknown	JJ
cord-007261-b5fgb9wf	29	23	.	.	.
cord-007261-b5fgb9wf	30	1	To	to	IN
cord-007261-b5fgb9wf	30	2	-	-	HYPH
cord-007261-b5fgb9wf	30	3	study	study	VB
cord-007261-b5fgb9wf	30	4	the	the	DT
cord-007261-b5fgb9wf	30	5	retention	retention	NN
cord-007261-b5fgb9wf	30	6	mechanism	mechanism	NN
cord-007261-b5fgb9wf	30	7	for	for	IN
cord-007261-b5fgb9wf	30	8	the	the	DT
cord-007261-b5fgb9wf	30	9	ER	ER	NNP
cord-007261-b5fgb9wf	30	10	membrane	membrane	NN
cord-007261-b5fgb9wf	30	11	proteins	protein	NNS
cord-007261-b5fgb9wf	30	12	without	without	IN
cord-007261-b5fgb9wf	30	13	the	the	DT
cord-007261-b5fgb9wf	30	14	KDEL	KDEL	NNP
cord-007261-b5fgb9wf	30	15	or	or	CC
cord-007261-b5fgb9wf	30	16	double	double	JJ
cord-007261-b5fgb9wf	30	17	-	-	HYPH
cord-007261-b5fgb9wf	30	18	lysine	lysine	NN
cord-007261-b5fgb9wf	30	19	motif	motif	NN
cord-007261-b5fgb9wf	30	20	,	,	,
cord-007261-b5fgb9wf	30	21	we	-PRON-	PRP
cord-007261-b5fgb9wf	30	22	chose	choose	VBD
cord-007261-b5fgb9wf	30	23	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	30	24	cytochrome	cytochrome	NNP
cord-007261-b5fgb9wf	31	1	P450s	P450s	NNP
cord-007261-b5fgb9wf	32	1	(	(	-LRB-
cord-007261-b5fgb9wf	32	2	P450s	P450s	NNP
cord-007261-b5fgb9wf	32	3	)	)	-RRB-
cord-007261-b5fgb9wf	32	4	.	.	.
cord-007261-b5fgb9wf	33	1	They	-PRON-	PRP
cord-007261-b5fgb9wf	33	2	are	be	VBP
cord-007261-b5fgb9wf	33	3	integral	integral	JJ
cord-007261-b5fgb9wf	33	4	membrane	membrane	NN
cord-007261-b5fgb9wf	33	5	proteins	protein	NNS
cord-007261-b5fgb9wf	33	6	localized	localize	VBN
cord-007261-b5fgb9wf	33	7	in	in	IN
cord-007261-b5fgb9wf	33	8	the	the	DT
cord-007261-b5fgb9wf	33	9	ER	ER	NNP
cord-007261-b5fgb9wf	33	10	,	,	,
cord-007261-b5fgb9wf	33	11	and	and	CC
cord-007261-b5fgb9wf	33	12	carry	carry	VB
cord-007261-b5fgb9wf	33	13	the	the	DT
cord-007261-b5fgb9wf	33	14	type	type	NN
cord-007261-b5fgb9wf	33	15	I	-PRON-	PRP
cord-007261-b5fgb9wf	33	16	signal	signal	NN
cord-007261-b5fgb9wf	33	17	-	-	HYPH
cord-007261-b5fgb9wf	33	18	anchor	anchor	NN
cord-007261-b5fgb9wf	33	19	sequence	sequence	NN
cord-007261-b5fgb9wf	33	20	at	at	IN
cord-007261-b5fgb9wf	33	21	their	-PRON-	PRP$
cord-007261-b5fgb9wf	33	22	N	n	CD
cord-007261-b5fgb9wf	33	23	-	-	HYPH
cord-007261-b5fgb9wf	33	24	terminus	terminus	NN
cord-007261-b5fgb9wf	33	25	,	,	,
cord-007261-b5fgb9wf	33	26	which	which	WDT
cord-007261-b5fgb9wf	33	27	functions	function	VBZ
cord-007261-b5fgb9wf	33	28	both	both	DT
cord-007261-b5fgb9wf	33	29	as	as	IN
cord-007261-b5fgb9wf	33	30	a	a	DT
cord-007261-b5fgb9wf	33	31	classical	classical	JJ
cord-007261-b5fgb9wf	33	32	signal	signal	NN
cord-007261-b5fgb9wf	33	33	sequence	sequence	NN
cord-007261-b5fgb9wf	33	34	and	and	CC
cord-007261-b5fgb9wf	33	35	as	as	IN
cord-007261-b5fgb9wf	33	36	a	a	DT
cord-007261-b5fgb9wf	33	37	stop	stop	VB
cord-007261-b5fgb9wf	33	38	-	-	HYPH
cord-007261-b5fgb9wf	33	39	transfer	transfer	NN
cord-007261-b5fgb9wf	33	40	sequence	sequence	NN
cord-007261-b5fgb9wf	33	41	(	(	-LRB-
cord-007261-b5fgb9wf	33	42	9	9	CD
cord-007261-b5fgb9wf	33	43	)	)	-RRB-
cord-007261-b5fgb9wf	33	44	(	(	-LRB-
cord-007261-b5fgb9wf	33	45	10	10	CD
cord-007261-b5fgb9wf	33	46	)	)	-RRB-
cord-007261-b5fgb9wf	33	47	(	(	-LRB-
cord-007261-b5fgb9wf	33	48	11	11	CD
cord-007261-b5fgb9wf	33	49	)	)	-RRB-
cord-007261-b5fgb9wf	33	50	.	.	.
cord-007261-b5fgb9wf	34	1	The	the	DT
cord-007261-b5fgb9wf	34	2	concerted	concerted	JJ
cord-007261-b5fgb9wf	34	3	action	action	NN
cord-007261-b5fgb9wf	34	4	of	of	IN
cord-007261-b5fgb9wf	34	5	this	this	DT
cord-007261-b5fgb9wf	34	6	signal	signal	NN
cord-007261-b5fgb9wf	34	7	-	-	HYPH
cord-007261-b5fgb9wf	34	8	anchor	anchor	NN
cord-007261-b5fgb9wf	34	9	sequence	sequence	NN
cord-007261-b5fgb9wf	34	10	and	and	CC
cord-007261-b5fgb9wf	34	11	the	the	DT
cord-007261-b5fgb9wf	34	12	signal	signal	NN
cord-007261-b5fgb9wf	34	13	recognition	recognition	NN
cord-007261-b5fgb9wf	34	14	particle	particle	NN
cord-007261-b5fgb9wf	34	15	targets	target	VBZ
cord-007261-b5fgb9wf	34	16	P450s	P450s	NNP
cord-007261-b5fgb9wf	34	17	to	to	IN
cord-007261-b5fgb9wf	34	18	the	the	DT
cord-007261-b5fgb9wf	34	19	ER	ER	NNP
cord-007261-b5fgb9wf	34	20	,	,	,
cord-007261-b5fgb9wf	34	21	and	and	CC
cord-007261-b5fgb9wf	34	22	the	the	DT
cord-007261-b5fgb9wf	34	23	P450s	P450s	NNP
cord-007261-b5fgb9wf	34	24	are	be	VBP
cord-007261-b5fgb9wf	34	25	inserted	insert	VBN
cord-007261-b5fgb9wf	34	26	into	into	IN
cord-007261-b5fgb9wf	34	27	the	the	DT
cord-007261-b5fgb9wf	34	28	membranes	membrane	NNS
cord-007261-b5fgb9wf	34	29	in	in	IN
cord-007261-b5fgb9wf	34	30	the	the	DT
cord-007261-b5fgb9wf	34	31	type	type	NN
cord-007261-b5fgb9wf	34	32	I	-PRON-	PRP
cord-007261-b5fgb9wf	34	33	(	(	-LRB-
cord-007261-b5fgb9wf	34	34	Nin	Nin	NNP
cord-007261-b5fgb9wf	34	35	-	-	HYPH
cord-007261-b5fgb9wf	34	36	Ccyt	Ccyt	NNP
cord-007261-b5fgb9wf	34	37	)	)	-RRB-
cord-007261-b5fgb9wf	34	38	orientation	orientation	NN
cord-007261-b5fgb9wf	34	39	{	{	-LRB-
cord-007261-b5fgb9wf	34	40	12	12	CD
cord-007261-b5fgb9wf	34	41	)	)	-RRB-
cord-007261-b5fgb9wf	34	42	.	.	.
cord-007261-b5fgb9wf	35	1	Although	although	IN
cord-007261-b5fgb9wf	35	2	the	the	DT
cord-007261-b5fgb9wf	35	3	insertion	insertion	NN
cord-007261-b5fgb9wf	35	4	of	of	IN
cord-007261-b5fgb9wf	35	5	P450s	P450s	NNP
cord-007261-b5fgb9wf	35	6	into	into	IN
cord-007261-b5fgb9wf	35	7	the	the	DT
cord-007261-b5fgb9wf	35	8	ER	ER	NNP
cord-007261-b5fgb9wf	35	9	has	have	VBZ
cord-007261-b5fgb9wf	35	10	been	be	VBN
cord-007261-b5fgb9wf	35	11	studied	study	VBN
cord-007261-b5fgb9wf	35	12	extensively	extensively	RB
cord-007261-b5fgb9wf	35	13	,	,	,
cord-007261-b5fgb9wf	35	14	the	the	DT
cord-007261-b5fgb9wf	35	15	mechanism	mechanism	NN
cord-007261-b5fgb9wf	35	16	by	by	IN
cord-007261-b5fgb9wf	35	17	which	which	WDT
cord-007261-b5fgb9wf	35	18	P450s	P450s	NNP
cord-007261-b5fgb9wf	35	19	become	become	VBP
cord-007261-b5fgb9wf	35	20	ER	ER	NNP
cord-007261-b5fgb9wf	35	21	residents	resident	NNS
cord-007261-b5fgb9wf	35	22	is	be	VBZ
cord-007261-b5fgb9wf	35	23	not	not	RB
cord-007261-b5fgb9wf	35	24	yet	yet	RB
cord-007261-b5fgb9wf	35	25	clear	clear	JJ
cord-007261-b5fgb9wf	35	26	.	.	.
cord-007261-b5fgb9wf	36	1	Although	although	IN
cord-007261-b5fgb9wf	36	2	P450s	p450s	PRP
cord-007261-b5fgb9wf	36	3	assume	assume	VBP
cord-007261-b5fgb9wf	36	4	the	the	DT
cord-007261-b5fgb9wf	36	5	type	type	NN
cord-007261-b5fgb9wf	36	6	I	-PRON-	PRP
cord-007261-b5fgb9wf	36	7	orientation	orientation	VBP
cord-007261-b5fgb9wf	36	8	in	in	IN
cord-007261-b5fgb9wf	36	9	the	the	DT
cord-007261-b5fgb9wf	36	10	ER	ER	NNP
cord-007261-b5fgb9wf	36	11	membrane	membrane	NN
cord-007261-b5fgb9wf	36	12	,	,	,
cord-007261-b5fgb9wf	36	13	no	no	DT
cord-007261-b5fgb9wf	36	14	double	double	JJ
cord-007261-b5fgb9wf	36	15	-	-	HYPH
cord-007261-b5fgb9wf	36	16	lysine	lysine	NN
cord-007261-b5fgb9wf	36	17	motif	motif	NN
cord-007261-b5fgb9wf	36	18	is	be	VBZ
cord-007261-b5fgb9wf	36	19	present	present	JJ
cord-007261-b5fgb9wf	36	20	in	in	IN
cord-007261-b5fgb9wf	36	21	their	-PRON-	PRP$
cord-007261-b5fgb9wf	36	22	C	c	NN
cord-007261-b5fgb9wf	36	23	-	-	HYPH
cord-007261-b5fgb9wf	36	24	terminus	terminus	NN
cord-007261-b5fgb9wf	36	25	.	.	.
cord-007261-b5fgb9wf	37	1	To	to	TO
cord-007261-b5fgb9wf	37	2	understand	understand	VB
cord-007261-b5fgb9wf	37	3	the	the	DT
cord-007261-b5fgb9wf	37	4	retention	retention	NN
cord-007261-b5fgb9wf	37	5	of	of	IN
cord-007261-b5fgb9wf	37	6	P450s	P450s	NNP
cord-007261-b5fgb9wf	37	7	in	in	IN
cord-007261-b5fgb9wf	37	8	the	the	DT
cord-007261-b5fgb9wf	37	9	ER	ER	NNP
cord-007261-b5fgb9wf	37	10	membrane	membrane	NN
cord-007261-b5fgb9wf	37	11	,	,	,
cord-007261-b5fgb9wf	37	12	in	in	IN
cord-007261-b5fgb9wf	37	13	this	this	DT
cord-007261-b5fgb9wf	37	14	study	study	NN
cord-007261-b5fgb9wf	37	15	we	-PRON-	PRP
cord-007261-b5fgb9wf	37	16	defined	define	VBD
cord-007261-b5fgb9wf	37	17	the	the	DT
cord-007261-b5fgb9wf	37	18	region	region	NN
cord-007261-b5fgb9wf	37	19	of	of	IN
cord-007261-b5fgb9wf	37	20	the	the	DT
cord-007261-b5fgb9wf	37	21	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	37	22	)	)	-RRB-
cord-007261-b5fgb9wf	37	23	molecule	molecule	NN
cord-007261-b5fgb9wf	37	24	that	that	WDT
cord-007261-b5fgb9wf	37	25	determines	determine	VBZ
cord-007261-b5fgb9wf	37	26	P450s	p450s	PRP
cord-007261-b5fgb9wf	37	27	'	'	``
cord-007261-b5fgb9wf	37	28	ER	er	NN
cord-007261-b5fgb9wf	37	29	residency	residency	NN
cord-007261-b5fgb9wf	37	30	.	.	.
cord-007261-b5fgb9wf	38	1	We	-PRON-	PRP
cord-007261-b5fgb9wf	38	2	constructed	construct	VBD
cord-007261-b5fgb9wf	38	3	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	38	4	proteins	protein	NNS
cord-007261-b5fgb9wf	38	5	,	,	,
cord-007261-b5fgb9wf	38	6	in	in	IN
cord-007261-b5fgb9wf	38	7	which	which	WDT
cord-007261-b5fgb9wf	38	8	the	the	DT
cord-007261-b5fgb9wf	38	9	whole	whole	JJ
cord-007261-b5fgb9wf	38	10	or	or	CC
cord-007261-b5fgb9wf	38	11	portions	portion	NNS
cord-007261-b5fgb9wf	38	12	of	of	IN
cord-007261-b5fgb9wf	38	13	the	the	DT
cord-007261-b5fgb9wf	38	14	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	38	15	)	)	-RRB-
cord-007261-b5fgb9wf	38	16	molecule	molecule	NN
cord-007261-b5fgb9wf	38	17	were	be	VBD
cord-007261-b5fgb9wf	38	18	attached	attach	VBN
cord-007261-b5fgb9wf	38	19	to	to	IN
cord-007261-b5fgb9wf	38	20	the	the	DT
cord-007261-b5fgb9wf	38	21	C	C	NNP
cord-007261-b5fgb9wf	38	22	-	-	HYPH
cord-007261-b5fgb9wf	38	23	terminus	terminus	NN
cord-007261-b5fgb9wf	38	24	of	of	IN
cord-007261-b5fgb9wf	38	25	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	38	26	Sec	Sec	NNP
cord-007261-b5fgb9wf	38	27	,	,	,
cord-007261-b5fgb9wf	38	28	a	a	DT
cord-007261-b5fgb9wf	38	29	secretory	secretory	JJ
cord-007261-b5fgb9wf	38	30	protein	protein	NN
cord-007261-b5fgb9wf	38	31	used	use	VBN
cord-007261-b5fgb9wf	38	32	as	as	IN
cord-007261-b5fgb9wf	38	33	the	the	DT
cord-007261-b5fgb9wf	38	34	reporter	reporter	NN
cord-007261-b5fgb9wf	38	35	.	.	.
cord-007261-b5fgb9wf	39	1	As	as	IN
cord-007261-b5fgb9wf	39	2	a	a	DT
cord-007261-b5fgb9wf	39	3	control	control	NN
cord-007261-b5fgb9wf	39	4	,	,	,
cord-007261-b5fgb9wf	39	5	other	other	JJ
cord-007261-b5fgb9wf	39	6	types	type	NNS
cord-007261-b5fgb9wf	39	7	of	of	IN
cord-007261-b5fgb9wf	39	8	fusion	fusion	NN
cord-007261-b5fgb9wf	39	9	proteins	protein	NNS
cord-007261-b5fgb9wf	39	10	were	be	VBD
cord-007261-b5fgb9wf	39	11	constructed	construct	VBN
cord-007261-b5fgb9wf	39	12	in	in	IN
cord-007261-b5fgb9wf	39	13	which	which	WDT
cord-007261-b5fgb9wf	39	14	the	the	DT
cord-007261-b5fgb9wf	39	15	C	C	NNP
cord-007261-b5fgb9wf	39	16	-	-	HYPH
cord-007261-b5fgb9wf	39	17	terminal	terminal	NN
cord-007261-b5fgb9wf	39	18	portion	portion	NN
cord-007261-b5fgb9wf	39	19	of	of	IN
cord-007261-b5fgb9wf	39	20	UDPglucronosyltransferase	UDPglucronosyltransferase	NNP
cord-007261-b5fgb9wf	39	21	,	,	,
cord-007261-b5fgb9wf	39	22	which	which	WDT
cord-007261-b5fgb9wf	39	23	contains	contain	VBZ
cord-007261-b5fgb9wf	39	24	the	the	DT
cord-007261-b5fgb9wf	39	25	double	double	JJ
cord-007261-b5fgb9wf	39	26	-	-	HYPH
cord-007261-b5fgb9wf	39	27	lysine	lysine	NN
cord-007261-b5fgb9wf	39	28	motif	motif	NN
cord-007261-b5fgb9wf	39	29	for	for	IN
cord-007261-b5fgb9wf	39	30	the	the	DT
cord-007261-b5fgb9wf	39	31	ER	ER	NNP
cord-007261-b5fgb9wf	39	32	retention	retention	NN
cord-007261-b5fgb9wf	39	33	,	,	,
cord-007261-b5fgb9wf	39	34	was	be	VBD
cord-007261-b5fgb9wf	39	35	attached	attach	VBN
cord-007261-b5fgb9wf	39	36	to	to	IN
cord-007261-b5fgb9wf	39	37	carboxyesterase	carboxyesterase	VB
cord-007261-b5fgb9wf	39	38	Sec	Sec	NNP
cord-007261-b5fgb9wf	39	39	.	.	.
cord-007261-b5fgb9wf	40	1	The	the	DT
cord-007261-b5fgb9wf	40	2	fusion	fusion	NN
cord-007261-b5fgb9wf	40	3	proteins	protein	NNS
cord-007261-b5fgb9wf	40	4	were	be	VBD
cord-007261-b5fgb9wf	40	5	expressed	express	VBN
cord-007261-b5fgb9wf	40	6	in	in	IN
cord-007261-b5fgb9wf	40	7	COS	COS	NNP
cord-007261-b5fgb9wf	40	8	cells	cell	NNS
cord-007261-b5fgb9wf	40	9	,	,	,
cord-007261-b5fgb9wf	40	10	and	and	CC
cord-007261-b5fgb9wf	40	11	the	the	DT
cord-007261-b5fgb9wf	40	12	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	40	13	localization	localization	NN
cord-007261-b5fgb9wf	40	14	of	of	IN
cord-007261-b5fgb9wf	40	15	the	the	DT
cord-007261-b5fgb9wf	40	16	fusion	fusion	NN
cord-007261-b5fgb9wf	40	17	proteins	protein	NNS
cord-007261-b5fgb9wf	40	18	was	be	VBD
cord-007261-b5fgb9wf	40	19	determined	determine	VBN
cord-007261-b5fgb9wf	40	20	.	.	.
cord-007261-b5fgb9wf	41	1	To	to	TO
cord-007261-b5fgb9wf	41	2	determine	determine	VB
cord-007261-b5fgb9wf	41	3	whether	whether	IN
cord-007261-b5fgb9wf	41	4	P450s	p450s	PRP
cord-007261-b5fgb9wf	41	5	are	be	VBP
cord-007261-b5fgb9wf	41	6	retained	retain	VBN
cord-007261-b5fgb9wf	41	7	permanently	permanently	RB
cord-007261-b5fgb9wf	41	8	in	in	IN
cord-007261-b5fgb9wf	41	9	the	the	DT
cord-007261-b5fgb9wf	41	10	ER	ER	NNP
cord-007261-b5fgb9wf	41	11	or	or	CC
cord-007261-b5fgb9wf	41	12	recycled	recycle	VBN
cord-007261-b5fgb9wf	41	13	between	between	IN
cord-007261-b5fgb9wf	41	14	the	the	DT
cord-007261-b5fgb9wf	41	15	ER	er	NN
cord-007261-b5fgb9wf	41	16	and	and	CC
cord-007261-b5fgb9wf	41	17	post	post	JJ
cord-007261-b5fgb9wf	41	18	-	-	JJ
cord-007261-b5fgb9wf	41	19	ER	er	JJ
cord-007261-b5fgb9wf	41	20	compartments	compartment	NNS
cord-007261-b5fgb9wf	41	21	through	through	IN
cord-007261-b5fgb9wf	41	22	the	the	DT
cord-007261-b5fgb9wf	41	23	"	"	``
cord-007261-b5fgb9wf	41	24	export	export	NN
cord-007261-b5fgb9wf	41	25	and	and	CC
cord-007261-b5fgb9wf	41	26	retrieval	retrieval	NN
cord-007261-b5fgb9wf	41	27	"	"	''
cord-007261-b5fgb9wf	41	28	process	process	NN
cord-007261-b5fgb9wf	41	29	,	,	,
cord-007261-b5fgb9wf	41	30	we	-PRON-	PRP
cord-007261-b5fgb9wf	41	31	chose	choose	VBD
cord-007261-b5fgb9wf	41	32	a	a	DT
cord-007261-b5fgb9wf	41	33	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	41	34	enzyme	enzyme	NN
cord-007261-b5fgb9wf	41	35	,	,	,
cord-007261-b5fgb9wf	41	36	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	41	37	D	d	NN
cord-007261-b5fgb9wf	41	38	,	,	,
cord-007261-b5fgb9wf	41	39	as	as	IN
cord-007261-b5fgb9wf	41	40	a	a	DT
cord-007261-b5fgb9wf	41	41	reporter	reporter	NN
cord-007261-b5fgb9wf	41	42	,	,	,
cord-007261-b5fgb9wf	41	43	which	which	WDT
cord-007261-b5fgb9wf	41	44	was	be	VBD
cord-007261-b5fgb9wf	41	45	fused	fuse	VBN
cord-007261-b5fgb9wf	41	46	to	to	IN
cord-007261-b5fgb9wf	41	47	the	the	DT
cord-007261-b5fgb9wf	41	48	N	n	NN
cord-007261-b5fgb9wf	41	49	-	-	HYPH
cord-007261-b5fgb9wf	41	50	terminus	terminus	NN
cord-007261-b5fgb9wf	41	51	of	of	IN
cord-007261-b5fgb9wf	41	52	P450	P450	NNPS
cord-007261-b5fgb9wf	41	53	at	at	IN
cord-007261-b5fgb9wf	41	54	the	the	DT
cord-007261-b5fgb9wf	41	55	DNA	dna	NN
cord-007261-b5fgb9wf	41	56	level	level	NN
cord-007261-b5fgb9wf	41	57	and	and	CC
cord-007261-b5fgb9wf	41	58	the	the	DT
cord-007261-b5fgb9wf	41	59	fusion	fusion	NN
cord-007261-b5fgb9wf	41	60	protein	protein	NN
cord-007261-b5fgb9wf	41	61	was	be	VBD
cord-007261-b5fgb9wf	41	62	expressed	express	VBN
cord-007261-b5fgb9wf	41	63	in	in	IN
cord-007261-b5fgb9wf	41	64	COS	COS	NNP
cord-007261-b5fgb9wf	41	65	cells	cell	NNS
cord-007261-b5fgb9wf	41	66	.	.	.
cord-007261-b5fgb9wf	42	1	The	the	DT
cord-007261-b5fgb9wf	42	2	results	result	NNS
cord-007261-b5fgb9wf	42	3	with	with	IN
cord-007261-b5fgb9wf	42	4	the	the	DT
cord-007261-b5fgb9wf	42	5	fusion	fusion	NN
cord-007261-b5fgb9wf	42	6	protein	protein	NN
cord-007261-b5fgb9wf	42	7	suggested	suggest	VBD
cord-007261-b5fgb9wf	42	8	that	that	IN
cord-007261-b5fgb9wf	42	9	the	the	DT
cord-007261-b5fgb9wf	42	10	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	42	11	segment	segment	NN
cord-007261-b5fgb9wf	42	12	of	of	IN
cord-007261-b5fgb9wf	42	13	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	42	14	)	)	-RRB-
cord-007261-b5fgb9wf	42	15	was	be	VBD
cord-007261-b5fgb9wf	42	16	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	42	17	for	for	IN
cord-007261-b5fgb9wf	42	18	the	the	DT
cord-007261-b5fgb9wf	42	19	ER	ER	NNP
cord-007261-b5fgb9wf	42	20	retention	retention	NN
cord-007261-b5fgb9wf	42	21	,	,	,
cord-007261-b5fgb9wf	42	22	which	which	WDT
cord-007261-b5fgb9wf	42	23	diverted	divert	VBD
cord-007261-b5fgb9wf	42	24	P450s	P450s	NNP
cord-007261-b5fgb9wf	42	25	from	from	IN
cord-007261-b5fgb9wf	42	26	the	the	DT
cord-007261-b5fgb9wf	42	27	export	export	NN
cord-007261-b5fgb9wf	42	28	and	and	CC
cord-007261-b5fgb9wf	42	29	recycling	recycle	VBG
cord-007261-b5fgb9wf	42	30	pathways	pathway	NNS
cord-007261-b5fgb9wf	42	31	to	to	TO
cord-007261-b5fgb9wf	42	32	be	be	VB
cord-007261-b5fgb9wf	42	33	retained	retain	VBN
cord-007261-b5fgb9wf	42	34	in	in	IN
cord-007261-b5fgb9wf	42	35	the	the	DT
cord-007261-b5fgb9wf	42	36	ER	ER	NNP
cord-007261-b5fgb9wf	42	37	.	.	.
cord-007261-b5fgb9wf	43	1	Plasmids	Plasmids	NNP
cord-007261-b5fgb9wf	43	2	-	-	:
cord-007261-b5fgb9wf	43	3	The	the	DT
cord-007261-b5fgb9wf	43	4	COS	COS	NNP
cord-007261-b5fgb9wf	43	5	cell	cell	NN
cord-007261-b5fgb9wf	43	6	expression	expression	NN
cord-007261-b5fgb9wf	43	7	vectors	vector	NNS
cord-007261-b5fgb9wf	43	8	were	be	VBD
cord-007261-b5fgb9wf	43	9	based	base	VBN
cord-007261-b5fgb9wf	43	10	on	on	IN
cord-007261-b5fgb9wf	43	11	plasmid	plasmid	NN
cord-007261-b5fgb9wf	43	12	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	43	13	,	,	,
cord-007261-b5fgb9wf	43	14	which	which	WDT
cord-007261-b5fgb9wf	43	15	was	be	VBD
cord-007261-b5fgb9wf	43	16	kindly	kindly	RB
cord-007261-b5fgb9wf	43	17	provided	provide	VBN
cord-007261-b5fgb9wf	43	18	by	by	IN
cord-007261-b5fgb9wf	43	19	Dr.	Dr.	NNP
cord-007261-b5fgb9wf	43	20	Y.	Y.	NNP
cord-007261-b5fgb9wf	43	21	Ikehara	Ikehara	NNP
cord-007261-b5fgb9wf	43	22	of	of	IN
cord-007261-b5fgb9wf	43	23	Fukuoka	Fukuoka	NNP
cord-007261-b5fgb9wf	43	24	University	University	NNP
cord-007261-b5fgb9wf	43	25	.	.	.
cord-007261-b5fgb9wf	44	1	This	this	DT
cord-007261-b5fgb9wf	44	2	plasmid	plasmid	NN
cord-007261-b5fgb9wf	44	3	contains	contain	VBZ
cord-007261-b5fgb9wf	44	4	the	the	DT
cord-007261-b5fgb9wf	44	5	SV40	SV40	NNP
cord-007261-b5fgb9wf	44	6	origin	origin	NN
cord-007261-b5fgb9wf	44	7	and	and	CC
cord-007261-b5fgb9wf	44	8	SV40	SV40	NNP
cord-007261-b5fgb9wf	44	9	early	early	JJ
cord-007261-b5fgb9wf	44	10	promoter	promoter	NN
cord-007261-b5fgb9wf	44	11	.	.	.
cord-007261-b5fgb9wf	45	1	A	a	DT
cord-007261-b5fgb9wf	45	2	cDNA	cdna	NN
cord-007261-b5fgb9wf	45	3	fragment	fragment	NN
cord-007261-b5fgb9wf	45	4	carrying	carry	VBG
cord-007261-b5fgb9wf	45	5	the	the	DT
cord-007261-b5fgb9wf	45	6	entire	entire	JJ
cord-007261-b5fgb9wf	45	7	coding	code	VBG
cord-007261-b5fgb9wf	45	8	sequence	sequence	NN
cord-007261-b5fgb9wf	45	9	of	of	IN
cord-007261-b5fgb9wf	45	10	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	45	11	Sec	Sec	NNP
cord-007261-b5fgb9wf	45	12	was	be	VBD
cord-007261-b5fgb9wf	45	13	excised	excise	VBN
cord-007261-b5fgb9wf	45	14	from	from	IN
cord-007261-b5fgb9wf	45	15	a	a	DT
cord-007261-b5fgb9wf	45	16	clone	clone	NN
cord-007261-b5fgb9wf	45	17	(	(	-LRB-
cord-007261-b5fgb9wf	45	18	8	8	CD
cord-007261-b5fgb9wf	45	19	-	-	HYPH
cord-007261-b5fgb9wf	45	20	1/2	1/2	CD
cord-007261-b5fgb9wf	45	21	-	-	SYM
cord-007261-b5fgb9wf	45	22	1	1	CD
cord-007261-b5fgb9wf	45	23	)	)	-RRB-
cord-007261-b5fgb9wf	45	24	(	(	-LRB-
cord-007261-b5fgb9wf	45	25	13	13	CD
cord-007261-b5fgb9wf	45	26	)	)	-RRB-
cord-007261-b5fgb9wf	45	27	by	by	IN
cord-007261-b5fgb9wf	45	28	EcoRI	EcoRI	NNP
cord-007261-b5fgb9wf	45	29	-	-	HYPH
cord-007261-b5fgb9wf	45	30	HuuHH	HuuHH	NNP
cord-007261-b5fgb9wf	45	31	digestion	digestion	NN
cord-007261-b5fgb9wf	45	32	.	.	.
cord-007261-b5fgb9wf	46	1	The	the	DT
cord-007261-b5fgb9wf	46	2	fragment	fragment	NN
cord-007261-b5fgb9wf	46	3	was	be	VBD
cord-007261-b5fgb9wf	46	4	blunt	blunt	JJ
cord-007261-b5fgb9wf	46	5	-	-	HYPH
cord-007261-b5fgb9wf	46	6	ended	ended	JJ
cord-007261-b5fgb9wf	46	7	and	and	CC
cord-007261-b5fgb9wf	46	8	ligated	ligate	VBN
cord-007261-b5fgb9wf	46	9	with	with	IN
cord-007261-b5fgb9wf	46	10	.EcoRI	.EcoRI	:
cord-007261-b5fgb9wf	46	11	linkers	linker	NNS
cord-007261-b5fgb9wf	46	12	,	,	,
cord-007261-b5fgb9wf	46	13	and	and	CC
cord-007261-b5fgb9wf	46	14	then	then	RB
cord-007261-b5fgb9wf	46	15	inserted	insert	VBD
cord-007261-b5fgb9wf	46	16	into	into	IN
cord-007261-b5fgb9wf	46	17	the	the	DT
cord-007261-b5fgb9wf	46	18	EcoRI	ecori	NN
cord-007261-b5fgb9wf	46	19	site	site	NN
cord-007261-b5fgb9wf	46	20	of	of	IN
cord-007261-b5fgb9wf	46	21	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	46	22	to	to	TO
cord-007261-b5fgb9wf	46	23	obtain	obtain	VB
cord-007261-b5fgb9wf	46	24	Sec	Sec	NNP
cord-007261-b5fgb9wf	46	25	.	.	.
cord-007261-b5fgb9wf	47	1	To	to	TO
cord-007261-b5fgb9wf	47	2	construct	construct	VB
cord-007261-b5fgb9wf	47	3	Sec	Sec	NNP
cord-007261-b5fgb9wf	47	4	/	/	SYM
cord-007261-b5fgb9wf	47	5	Ml	Ml	NNP
cord-007261-b5fgb9wf	47	6	,	,	,
cord-007261-b5fgb9wf	47	7	the	the	DT
cord-007261-b5fgb9wf	47	8	cDNA	cdna	NN
cord-007261-b5fgb9wf	47	9	fragment	fragment	NN
cord-007261-b5fgb9wf	47	10	of	of	IN
cord-007261-b5fgb9wf	47	11	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	47	12	)	)	-RRB-
cord-007261-b5fgb9wf	47	13	was	be	VBD
cord-007261-b5fgb9wf	47	14	excised	excise	VBN
cord-007261-b5fgb9wf	47	15	by	by	IN
cord-007261-b5fgb9wf	47	16	BamHI	bamhi	NN
cord-007261-b5fgb9wf	47	17	digestion	digestion	NN
cord-007261-b5fgb9wf	47	18	from	from	IN
cord-007261-b5fgb9wf	47	19	pD2Ml	pD2Ml	NNP
cord-007261-b5fgb9wf	47	20	(	(	-LRB-
cord-007261-b5fgb9wf	47	21	14	14	CD
cord-007261-b5fgb9wf	47	22	)	)	-RRB-
cord-007261-b5fgb9wf	47	23	,	,	,
cord-007261-b5fgb9wf	47	24	and	and	CC
cord-007261-b5fgb9wf	47	25	then	then	RB
cord-007261-b5fgb9wf	47	26	the	the	DT
cord-007261-b5fgb9wf	47	27	fragment	fragment	NN
cord-007261-b5fgb9wf	47	28	was	be	VBD
cord-007261-b5fgb9wf	47	29	inserted	insert	VBN
cord-007261-b5fgb9wf	47	30	into	into	IN
cord-007261-b5fgb9wf	47	31	the	the	DT
cord-007261-b5fgb9wf	47	32	Bctl	Bctl	NNP
cord-007261-b5fgb9wf	47	33	site	site	NN
cord-007261-b5fgb9wf	47	34	of	of	IN
cord-007261-b5fgb9wf	47	35	Sec	Sec	NNP
cord-007261-b5fgb9wf	47	36	.	.	.
cord-007261-b5fgb9wf	48	1	To	to	TO
cord-007261-b5fgb9wf	48	2	construct	construct	VB
cord-007261-b5fgb9wf	48	3	Sec	Sec	NNP
cord-007261-b5fgb9wf	48	4	/	/	SYM
cord-007261-b5fgb9wf	48	5	Mi	Mi	NNP
cord-007261-b5fgb9wf	48	6	-	-	HYPH
cord-007261-b5fgb9wf	48	7	Pro	Pro	NNP
cord-007261-b5fgb9wf	48	8	,	,	,
cord-007261-b5fgb9wf	48	9	Sec/	Sec/	NNP
cord-007261-b5fgb9wf	48	10	Ml	Ml	NNP
cord-007261-b5fgb9wf	48	11	-	-	HYPH
cord-007261-b5fgb9wf	48	12	TM	TM	NNP
cord-007261-b5fgb9wf	48	13	,	,	,
cord-007261-b5fgb9wf	48	14	Sec	Sec	NNP
cord-007261-b5fgb9wf	48	15	/	/	SYM
cord-007261-b5fgb9wf	48	16	Ml	Ml	NNP
cord-007261-b5fgb9wf	48	17	-	-	HYPH
cord-007261-b5fgb9wf	48	18	H	H	NNP
cord-007261-b5fgb9wf	48	19	,	,	,
cord-007261-b5fgb9wf	48	20	and	and	CC
cord-007261-b5fgb9wf	48	21	Sec	Sec	NNP
cord-007261-b5fgb9wf	48	22	/	/	SYM
cord-007261-b5fgb9wf	48	23	Ml	Ml	NNP
cord-007261-b5fgb9wf	48	24	-	-	HYPH
cord-007261-b5fgb9wf	48	25	Q	Q	NNP
cord-007261-b5fgb9wf	48	26	,	,	,
cord-007261-b5fgb9wf	48	27	the	the	DT
cord-007261-b5fgb9wf	48	28	fragments	fragment	NNS
cord-007261-b5fgb9wf	48	29	encoding	encode	VBG
cord-007261-b5fgb9wf	48	30	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	48	31	)	)	-RRB-
cord-007261-b5fgb9wf	49	1	amino	amino	JJ
cord-007261-b5fgb9wf	49	2	acids	acid	NNS
cord-007261-b5fgb9wf	49	3	1	1	CD
cord-007261-b5fgb9wf	49	4	-	-	SYM
cord-007261-b5fgb9wf	49	5	37	37	CD
cord-007261-b5fgb9wf	49	6	,	,	,
cord-007261-b5fgb9wf	49	7	1	1	CD
cord-007261-b5fgb9wf	49	8	-	-	SYM
cord-007261-b5fgb9wf	49	9	20	20	CD
cord-007261-b5fgb9wf	49	10	,	,	,
cord-007261-b5fgb9wf	49	11	1	1	CD
cord-007261-b5fgb9wf	49	12	-	-	SYM
cord-007261-b5fgb9wf	49	13	11	11	CD
cord-007261-b5fgb9wf	49	14	,	,	,
cord-007261-b5fgb9wf	49	15	and	and	CC
cord-007261-b5fgb9wf	49	16	1	1	CD
cord-007261-b5fgb9wf	49	17	-	-	SYM
cord-007261-b5fgb9wf	49	18	7	7	CD
cord-007261-b5fgb9wf	49	19	were	be	VBD
cord-007261-b5fgb9wf	49	20	amplified	amplify	VBN
cord-007261-b5fgb9wf	49	21	from	from	IN
cord-007261-b5fgb9wf	49	22	pMl	pMl	NNP
cord-007261-b5fgb9wf	49	23	(	(	-LRB-
cord-007261-b5fgb9wf	49	24	15	15	CD
cord-007261-b5fgb9wf	49	25	)	)	-RRB-
cord-007261-b5fgb9wf	49	26	by	by	IN
cord-007261-b5fgb9wf	49	27	the	the	DT
cord-007261-b5fgb9wf	49	28	polymerase	polymerase	NN
cord-007261-b5fgb9wf	49	29	chain	chain	NN
cord-007261-b5fgb9wf	49	30	reaction	reaction	NN
cord-007261-b5fgb9wf	49	31	(	(	-LRB-
cord-007261-b5fgb9wf	49	32	PCR	PCR	NNP
cord-007261-b5fgb9wf	49	33	)	)	-RRB-
cord-007261-b5fgb9wf	49	34	using	use	VBG
cord-007261-b5fgb9wf	49	35	a	a	DT
cord-007261-b5fgb9wf	49	36	common	common	JJ
cord-007261-b5fgb9wf	49	37	5	5	CD
cord-007261-b5fgb9wf	49	38	'	'	''
cord-007261-b5fgb9wf	49	39	PCR	pcr	NN
cord-007261-b5fgb9wf	49	40	primer	primer	NN
cord-007261-b5fgb9wf	49	41	,	,	,
cord-007261-b5fgb9wf	49	42	5'-GACTCTAGA	5'-GACTCTAGA	NNP
cord-007261-b5fgb9wf	49	43	-	-	HYPH
cord-007261-b5fgb9wf	49	44	GGATCCAGTCCTAGT-3	GGATCCAGTCCTAGT-3	NNP
cord-007261-b5fgb9wf	49	45	'	'	''
cord-007261-b5fgb9wf	49	46	(	(	-LRB-
cord-007261-b5fgb9wf	49	47	a	a	DT
cord-007261-b5fgb9wf	49	48	BamHI	bamhi	NN
cord-007261-b5fgb9wf	49	49	site	site	NN
cord-007261-b5fgb9wf	49	50	is	be	VBZ
cord-007261-b5fgb9wf	49	51	underlined	underline	VBN
cord-007261-b5fgb9wf	49	52	)	)	-RRB-
cord-007261-b5fgb9wf	49	53	,	,	,
cord-007261-b5fgb9wf	49	54	and	and	CC
cord-007261-b5fgb9wf	49	55	3	3	CD
cord-007261-b5fgb9wf	49	56	'	'	''
cord-007261-b5fgb9wf	49	57	PCR	PCR	NNP
cord-007261-b5fgb9wf	49	58	primers	primer	NNS
cord-007261-b5fgb9wf	49	59	,	,	,
cord-007261-b5fgb9wf	49	60	5	5	CD
cord-007261-b5fgb9wf	49	61	'	'	POS
cord-007261-b5fgb9wf	49	62	-CCTCTCCCATAATGATCAAAC	-cctctcccataatgatcaaac	ADD
cord-007261-b5fgb9wf	49	63	-	-	HYPH
cord-007261-b5fgb9wf	49	64	AACCTT-3	AACCTT-3	NNP
cord-007261-b5fgb9wf	49	65	'	'	''
cord-007261-b5fgb9wf	49	66	for	for	IN
cord-007261-b5fgb9wf	49	67	the	the	DT
cord-007261-b5fgb9wf	49	68	1	1	CD
cord-007261-b5fgb9wf	49	69	-	-	SYM
cord-007261-b5fgb9wf	49	70	37	37	CD
cord-007261-b5fgb9wf	49	71	fragment	fragment	NN
cord-007261-b5fgb9wf	49	72	,	,	,
cord-007261-b5fgb9wf	49	73	5'-TCACTCTGGTAA	5'-tcactctggtaa	ADD
cord-007261-b5fgb9wf	49	74	-	-	HYPH
cord-007261-b5fgb9wf	49	75	TGATCATTTGGGAGA-3	TGATCATTTGGGAGA-3	NNP
cord-007261-b5fgb9wf	49	76	'	'	''
cord-007261-b5fgb9wf	49	77	for	for	IN
cord-007261-b5fgb9wf	49	78	the	the	DT
cord-007261-b5fgb9wf	49	79	1	1	CD
cord-007261-b5fgb9wf	49	80	-	-	SYM
cord-007261-b5fgb9wf	49	81	20	20	CD
cord-007261-b5fgb9wf	49	82	fragment	fragment	NN
cord-007261-b5fgb9wf	49	83	,	,	,
cord-007261-b5fgb9wf	49	84	5'-CTC	5'-ctc	CD
cord-007261-b5fgb9wf	49	85	-	-	SYM
cord-007261-b5fgb9wf	49	86	ACTCTCTAATGATCACTTCTCCTC-3	ACTCTCTAATGATCACTTCTCCTC-3	NNP
cord-007261-b5fgb9wf	49	87	'	'	''
cord-007261-b5fgb9wf	49	88	for	for	IN
cord-007261-b5fgb9wf	49	89	the	the	DT
cord-007261-b5fgb9wf	49	90	1	1	CD
cord-007261-b5fgb9wf	49	91	-	-	SYM
cord-007261-b5fgb9wf	49	92	11	11	CD
cord-007261-b5fgb9wf	49	93	fragment	fragment	NN
cord-007261-b5fgb9wf	49	94	,	,	,
cord-007261-b5fgb9wf	49	95	and	and	CC
cord-007261-b5fgb9wf	49	96	5^CTCACTCTCTAATGATCACTCTCCTCT-3	5^ctcactctctaatgatcactctcctct-3	CD
cord-007261-b5fgb9wf	49	97	'	'	''
cord-007261-b5fgb9wf	49	98	for	for	IN
cord-007261-b5fgb9wf	49	99	the	the	DT
cord-007261-b5fgb9wf	49	100	1	1	CD
cord-007261-b5fgb9wf	49	101	-	-	SYM
cord-007261-b5fgb9wf	49	102	7	7	CD
cord-007261-b5fgb9wf	49	103	fragment	fragment	NN
cord-007261-b5fgb9wf	49	104	(	(	-LRB-
cord-007261-b5fgb9wf	49	105	Bell	Bell	NNP
cord-007261-b5fgb9wf	49	106	site	site	NN
cord-007261-b5fgb9wf	49	107	is	be	VBZ
cord-007261-b5fgb9wf	49	108	underlined	underline	VBN
cord-007261-b5fgb9wf	49	109	)	)	-RRB-
cord-007261-b5fgb9wf	49	110	.	.	.
cord-007261-b5fgb9wf	50	1	Each	each	DT
cord-007261-b5fgb9wf	50	2	resulting	result	VBG
cord-007261-b5fgb9wf	50	3	fragment	fragment	NN
cord-007261-b5fgb9wf	50	4	was	be	VBD
cord-007261-b5fgb9wf	50	5	digested	digest	VBN
cord-007261-b5fgb9wf	50	6	with	with	IN
cord-007261-b5fgb9wf	50	7	BamHI	BamHI	NNP
cord-007261-b5fgb9wf	50	8	and	and	CC
cord-007261-b5fgb9wf	50	9	Bell	Bell	NNP
cord-007261-b5fgb9wf	50	10	,	,	,
cord-007261-b5fgb9wf	50	11	and	and	CC
cord-007261-b5fgb9wf	50	12	then	then	RB
cord-007261-b5fgb9wf	50	13	inserted	insert	VBD
cord-007261-b5fgb9wf	50	14	into	into	IN
cord-007261-b5fgb9wf	50	15	the	the	DT
cord-007261-b5fgb9wf	50	16	Bell	Bell	NNP
cord-007261-b5fgb9wf	50	17	site	site	NN
cord-007261-b5fgb9wf	50	18	of	of	IN
cord-007261-b5fgb9wf	50	19	Sec	Sec	NNP
cord-007261-b5fgb9wf	50	20	.	.	.
cord-007261-b5fgb9wf	51	1	To	to	TO
cord-007261-b5fgb9wf	51	2	construct	construct	VB
cord-007261-b5fgb9wf	51	3	Sec	Sec	NNP
cord-007261-b5fgb9wf	51	4	/	/	SYM
cord-007261-b5fgb9wf	51	5	GT	GT	NNP
cord-007261-b5fgb9wf	51	6	and	and	CC
cord-007261-b5fgb9wf	51	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	51	8	/	/	SYM
cord-007261-b5fgb9wf	51	9	GTd	GTd	NNS
cord-007261-b5fgb9wf	51	10	,	,	,
cord-007261-b5fgb9wf	51	11	the	the	DT
cord-007261-b5fgb9wf	51	12	fragments	fragment	NNS
cord-007261-b5fgb9wf	51	13	containing	contain	VBG
cord-007261-b5fgb9wf	51	14	UDP	udp	NN
cord-007261-b5fgb9wf	51	15	-	-	HYPH
cord-007261-b5fgb9wf	51	16	glucronosyltransferase	glucronosyltransferase	NN
cord-007261-b5fgb9wf	51	17	amino	amino	JJ
cord-007261-b5fgb9wf	51	18	acids	acid	NNS
cord-007261-b5fgb9wf	51	19	435	435	CD
cord-007261-b5fgb9wf	51	20	-	-	SYM
cord-007261-b5fgb9wf	51	21	476	476	CD
cord-007261-b5fgb9wf	51	22	and	and	CC
cord-007261-b5fgb9wf	51	23	435	435	CD
cord-007261-b5fgb9wf	51	24	-	-	SYM
cord-007261-b5fgb9wf	51	25	467	467	CD
cord-007261-b5fgb9wf	51	26	were	be	VBD
cord-007261-b5fgb9wf	51	27	amplified	amplify	VBN
cord-007261-b5fgb9wf	51	28	from	from	IN
cord-007261-b5fgb9wf	51	29	pYGT	pygt	JJ
cord-007261-b5fgb9wf	51	30	(	(	-LRB-
cord-007261-b5fgb9wf	51	31	a	a	DT
cord-007261-b5fgb9wf	51	32	gift	gift	NN
cord-007261-b5fgb9wf	51	33	from	from	IN
cord-007261-b5fgb9wf	51	34	Dr.	Dr.	NNP
cord-007261-b5fgb9wf	51	35	Yuasa	Yuasa	NNP
cord-007261-b5fgb9wf	51	36	of	of	IN
cord-007261-b5fgb9wf	51	37	Rakuno	Rakuno	NNP
cord-007261-b5fgb9wf	51	38	Gakuen	Gakuen	NNP
cord-007261-b5fgb9wf	51	39	University	University	NNP
cord-007261-b5fgb9wf	51	40	)	)	-RRB-
cord-007261-b5fgb9wf	51	41	,	,	,
cord-007261-b5fgb9wf	51	42	which	which	WDT
cord-007261-b5fgb9wf	51	43	contains	contain	VBZ
cord-007261-b5fgb9wf	51	44	the	the	DT
cord-007261-b5fgb9wf	51	45	cDNA	cdna	NN
cord-007261-b5fgb9wf	51	46	sequence	sequence	NN
cord-007261-b5fgb9wf	51	47	encoding	encode	VBG
cord-007261-b5fgb9wf	51	48	the	the	DT
cord-007261-b5fgb9wf	51	49	C	C	NNP
cord-007261-b5fgb9wf	51	50	-	-	HYPH
cord-007261-b5fgb9wf	51	51	terminal	terminal	NN
cord-007261-b5fgb9wf	51	52	476	476	CD
cord-007261-b5fgb9wf	51	53	amino	amino	JJ
cord-007261-b5fgb9wf	51	54	acids	acid	NNS
cord-007261-b5fgb9wf	51	55	of	of	IN
cord-007261-b5fgb9wf	51	56	rat	rat	NN
cord-007261-b5fgb9wf	51	57	UDP	UDP	NNP
cord-007261-b5fgb9wf	51	58	-	-	HYPH
cord-007261-b5fgb9wf	51	59	GT	GT	NNP
cord-007261-b5fgb9wf	51	60	,	,	,
cord-007261-b5fgb9wf	51	61	using	use	VBG
cord-007261-b5fgb9wf	51	62	a	a	DT
cord-007261-b5fgb9wf	51	63	5	5	CD
cord-007261-b5fgb9wf	51	64	'	'	''
cord-007261-b5fgb9wf	51	65	PCR	pcr	NN
cord-007261-b5fgb9wf	51	66	primer	primer	NN
cord-007261-b5fgb9wf	51	67	for	for	IN
cord-007261-b5fgb9wf	51	68	both	both	DT
cord-007261-b5fgb9wf	51	69	fragments	fragment	NNS
cord-007261-b5fgb9wf	51	70	,	,	,
cord-007261-b5fgb9wf	51	71	5'-ACCTCCCCTGGGATCCGTACCACT-3	5'-acctcccctgggatccgtaccact-3	CD
cord-007261-b5fgb9wf	51	72	'	'	NNS
cord-007261-b5fgb9wf	51	73	,	,	,
cord-007261-b5fgb9wf	51	74	and	and	CC
cord-007261-b5fgb9wf	51	75	a	a	DT
cord-007261-b5fgb9wf	51	76	3	3	CD
cord-007261-b5fgb9wf	51	77	'	'	''
cord-007261-b5fgb9wf	51	78	PCR	pcr	NN
cord-007261-b5fgb9wf	51	79	primer	primer	NN
cord-007261-b5fgb9wf	51	80	,	,	,
cord-007261-b5fgb9wf	51	81	5'-CATTGTCATG^ATCCCTACTCATTCTT-3	5'-cattgtcatg^atccctactcattctt-3	CD
cord-007261-b5fgb9wf	51	82	'	'	''
cord-007261-b5fgb9wf	51	83	for	for	IN
cord-007261-b5fgb9wf	51	84	the	the	DT
cord-007261-b5fgb9wf	51	85	435	435	CD
cord-007261-b5fgb9wf	51	86	-	-	SYM
cord-007261-b5fgb9wf	51	87	476	476	CD
cord-007261-b5fgb9wf	51	88	fragment	fragment	NN
cord-007261-b5fgb9wf	51	89	,	,	,
cord-007261-b5fgb9wf	51	90	and	and	CC
cord-007261-b5fgb9wf	51	91	5'-CTTTTGCTTTTAGGA	5'-cttttgcttttagga	CD
cord-007261-b5fgb9wf	51	92	-	-	HYPH
cord-007261-b5fgb9wf	51	93	TCCGAATCGGTA-3	TCCGAATCGGTA-3	NNP
cord-007261-b5fgb9wf	51	94	'	'	''
cord-007261-b5fgb9wf	51	95	for	for	IN
cord-007261-b5fgb9wf	51	96	the	the	DT
cord-007261-b5fgb9wf	51	97	435	435	CD
cord-007261-b5fgb9wf	51	98	-	-	SYM
cord-007261-b5fgb9wf	51	99	467	467	CD
cord-007261-b5fgb9wf	51	100	fragment	fragment	NN
cord-007261-b5fgb9wf	51	101	(	(	-LRB-
cord-007261-b5fgb9wf	51	102	BamHI	BamHI	NNP
cord-007261-b5fgb9wf	51	103	site	site	NN
cord-007261-b5fgb9wf	51	104	is	be	VBZ
cord-007261-b5fgb9wf	51	105	underlined	underline	VBN
cord-007261-b5fgb9wf	51	106	)	)	-RRB-
cord-007261-b5fgb9wf	51	107	.	.	.
cord-007261-b5fgb9wf	52	1	Each	each	DT
cord-007261-b5fgb9wf	52	2	resulting	result	VBG
cord-007261-b5fgb9wf	52	3	fragment	fragment	NN
cord-007261-b5fgb9wf	52	4	was	be	VBD
cord-007261-b5fgb9wf	52	5	digested	digest	VBN
cord-007261-b5fgb9wf	52	6	with	with	IN
cord-007261-b5fgb9wf	52	7	BamHI	bamhi	NN
cord-007261-b5fgb9wf	52	8	and	and	CC
cord-007261-b5fgb9wf	52	9	then	then	RB
cord-007261-b5fgb9wf	52	10	inserted	insert	VBD
cord-007261-b5fgb9wf	52	11	into	into	IN
cord-007261-b5fgb9wf	52	12	the	the	DT
cord-007261-b5fgb9wf	52	13	Bell	Bell	NNP
cord-007261-b5fgb9wf	52	14	site	site	NN
cord-007261-b5fgb9wf	52	15	of	of	IN
cord-007261-b5fgb9wf	52	16	Sec	Sec	NNP
cord-007261-b5fgb9wf	52	17	.	.	.
cord-007261-b5fgb9wf	53	1	To	to	TO
cord-007261-b5fgb9wf	53	2	construct	construct	VB
cord-007261-b5fgb9wf	53	3	Ml	Ml	NNP
cord-007261-b5fgb9wf	53	4	and	and	CC
cord-007261-b5fgb9wf	53	5	CathD	CathD	NNP
cord-007261-b5fgb9wf	53	6	,	,	,
cord-007261-b5fgb9wf	53	7	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	53	8	)	)	-RRB-
cord-007261-b5fgb9wf	53	9	and	and	CC
cord-007261-b5fgb9wf	53	10	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	53	11	D	d	NN
cord-007261-b5fgb9wf	53	12	cDNA	cdna	NN
cord-007261-b5fgb9wf	53	13	fragments	fragment	NNS
cord-007261-b5fgb9wf	53	14	were	be	VBD
cord-007261-b5fgb9wf	53	15	excised	excise	VBN
cord-007261-b5fgb9wf	53	16	by	by	IN
cord-007261-b5fgb9wf	53	17	.EcoRI	.EcoRI	.
cord-007261-b5fgb9wf	54	1	digestion	digestion	NN
cord-007261-b5fgb9wf	54	2	from	from	IN
cord-007261-b5fgb9wf	54	3	pD2Ml	pD2Ml	NNP
cord-007261-b5fgb9wf	55	1	and	and	CC
cord-007261-b5fgb9wf	55	2	pCat-1	pcat-1	XX
cord-007261-b5fgb9wf	55	3	,	,	,
cord-007261-b5fgb9wf	55	4	and	and	CC
cord-007261-b5fgb9wf	55	5	then	then	RB
cord-007261-b5fgb9wf	55	6	inserted	insert	VBD
cord-007261-b5fgb9wf	55	7	into	into	IN
cord-007261-b5fgb9wf	55	8	the	the	DT
cord-007261-b5fgb9wf	55	9	EcoRI	ecori	NN
cord-007261-b5fgb9wf	55	10	site	site	NN
cord-007261-b5fgb9wf	55	11	of	of	IN
cord-007261-b5fgb9wf	55	12	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	55	13	.	.	.
cord-007261-b5fgb9wf	56	1	To	to	TO
cord-007261-b5fgb9wf	56	2	construct	construct	VB
cord-007261-b5fgb9wf	56	3	CathD	CathD	NNP
cord-007261-b5fgb9wf	56	4	/	/	SYM
cord-007261-b5fgb9wf	56	5	Ml	Ml	NNP
cord-007261-b5fgb9wf	56	6	,	,	,
cord-007261-b5fgb9wf	56	7	the	the	DT
cord-007261-b5fgb9wf	56	8	BamHI	BamHI	NNP
cord-007261-b5fgb9wf	56	9	site	site	NN
cord-007261-b5fgb9wf	56	10	of	of	IN
cord-007261-b5fgb9wf	56	11	pCat-1	pCat-1	NNP
cord-007261-b5fgb9wf	56	12	was	be	VBD
cord-007261-b5fgb9wf	56	13	shifted	shift	VBN
cord-007261-b5fgb9wf	56	14	from	from	IN
cord-007261-b5fgb9wf	56	15	bp	bp	NNP
cord-007261-b5fgb9wf	56	16	position	position	NN
cord-007261-b5fgb9wf	56	17	1143	1143	CD
cord-007261-b5fgb9wf	56	18	to	to	IN
cord-007261-b5fgb9wf	56	19	1144	1144	CD
cord-007261-b5fgb9wf	56	20	by	by	IN
cord-007261-b5fgb9wf	56	21	in	in	IN
cord-007261-b5fgb9wf	56	22	vitro	vitro	FW
cord-007261-b5fgb9wf	56	23	site	site	NN
cord-007261-b5fgb9wf	56	24	-	-	HYPH
cord-007261-b5fgb9wf	56	25	directed	direct	VBN
cord-007261-b5fgb9wf	56	26	mutagenesis	mutagenesis	NN
cord-007261-b5fgb9wf	56	27	.	.	.
cord-007261-b5fgb9wf	57	1	A	a	DT
cord-007261-b5fgb9wf	57	2	cDNA	cdna	NN
cord-007261-b5fgb9wf	57	3	fragment	fragment	NN
cord-007261-b5fgb9wf	57	4	coding	code	VBG
cord-007261-b5fgb9wf	57	5	the	the	DT
cord-007261-b5fgb9wf	57	6	N	n	CD
cord-007261-b5fgb9wf	57	7	-	-	HYPH
cord-007261-b5fgb9wf	57	8	terminal	terminal	NN
cord-007261-b5fgb9wf	57	9	378	378	CD
cord-007261-b5fgb9wf	57	10	amino	amino	JJ
cord-007261-b5fgb9wf	57	11	acids	acid	NNS
cord-007261-b5fgb9wf	57	12	of	of	IN
cord-007261-b5fgb9wf	57	13	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	57	14	D	d	NN
cord-007261-b5fgb9wf	57	15	was	be	VBD
cord-007261-b5fgb9wf	57	16	excised	excise	VBN
cord-007261-b5fgb9wf	57	17	from	from	IN
cord-007261-b5fgb9wf	57	18	the	the	DT
cord-007261-b5fgb9wf	57	19	mutated	mutate	VBN
cord-007261-b5fgb9wf	57	20	pCat-1	pcat-1	FW
cord-007261-b5fgb9wf	57	21	by	by	IN
cord-007261-b5fgb9wf	57	22	digestion	digestion	NN
cord-007261-b5fgb9wf	57	23	with	with	IN
cord-007261-b5fgb9wf	57	24	EcoRI	ecori	NN
cord-007261-b5fgb9wf	57	25	and	and	CC
cord-007261-b5fgb9wf	57	26	BamHI	bamhi	NN
cord-007261-b5fgb9wf	57	27	.	.	.
cord-007261-b5fgb9wf	58	1	The	the	DT
cord-007261-b5fgb9wf	58	2	fragment	fragment	NN
cord-007261-b5fgb9wf	58	3	was	be	VBD
cord-007261-b5fgb9wf	58	4	then	then	RB
cord-007261-b5fgb9wf	58	5	ligated	ligate	VBN
cord-007261-b5fgb9wf	58	6	into	into	IN
cord-007261-b5fgb9wf	58	7	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	58	8	digested	digest	VBN
cord-007261-b5fgb9wf	58	9	with	with	IN
cord-007261-b5fgb9wf	58	10	EcoRI	ecori	NN
cord-007261-b5fgb9wf	58	11	and	and	CC
cord-007261-b5fgb9wf	58	12	BamHI	bamhi	NN
cord-007261-b5fgb9wf	58	13	.	.	.
cord-007261-b5fgb9wf	59	1	The	the	DT
cord-007261-b5fgb9wf	59	2	resultant	resultant	JJ
cord-007261-b5fgb9wf	59	3	plasmid	plasmid	NN
cord-007261-b5fgb9wf	59	4	was	be	VBD
cord-007261-b5fgb9wf	59	5	digested	digest	VBN
cord-007261-b5fgb9wf	59	6	with	with	IN
cord-007261-b5fgb9wf	59	7	BamHI	BamHI	NNP
cord-007261-b5fgb9wf	59	8	,	,	,
cord-007261-b5fgb9wf	59	9	and	and	CC
cord-007261-b5fgb9wf	59	10	then	then	RB
cord-007261-b5fgb9wf	59	11	ligated	ligate	VBD
cord-007261-b5fgb9wf	59	12	with	with	IN
cord-007261-b5fgb9wf	59	13	the	the	DT
cord-007261-b5fgb9wf	59	14	DNA	dna	NN
cord-007261-b5fgb9wf	59	15	fragment	fragment	NN
cord-007261-b5fgb9wf	59	16	containing	contain	VBG
cord-007261-b5fgb9wf	59	17	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	59	18	)	)	-RRB-
cord-007261-b5fgb9wf	59	19	that	that	WDT
cord-007261-b5fgb9wf	59	20	had	have	VBD
cord-007261-b5fgb9wf	59	21	been	be	VBN
cord-007261-b5fgb9wf	59	22	obtained	obtain	VBN
cord-007261-b5fgb9wf	59	23	from	from	IN
cord-007261-b5fgb9wf	59	24	pD2Ml	pD2Ml	NNP
cord-007261-b5fgb9wf	59	25	by	by	IN
cord-007261-b5fgb9wf	59	26	BamHI	bamhi	NN
cord-007261-b5fgb9wf	59	27	digestion	digestion	NN
cord-007261-b5fgb9wf	59	28	.	.	.
cord-007261-b5fgb9wf	60	1	Antibodies	antibody	NNS
cord-007261-b5fgb9wf	60	2	-	-	HYPH
cord-007261-b5fgb9wf	60	3	Rabbit	Rabbit	NNP
cord-007261-b5fgb9wf	61	1	anti	anti	JJ
cord-007261-b5fgb9wf	61	2	-	-	JJ
cord-007261-b5fgb9wf	61	3	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	61	4	(	(	-LRB-
cord-007261-b5fgb9wf	61	5	anti	anti	JJ
cord-007261-b5fgb9wf	61	6	-	-	JJ
cord-007261-b5fgb9wf	61	7	Est	est	JJ
cord-007261-b5fgb9wf	61	8	)	)	-RRB-
cord-007261-b5fgb9wf	61	9	antibodies	antibody	NNS
cord-007261-b5fgb9wf	61	10	were	be	VBD
cord-007261-b5fgb9wf	61	11	prepared	prepare	VBN
cord-007261-b5fgb9wf	61	12	as	as	IN
cord-007261-b5fgb9wf	61	13	described	describe	VBN
cord-007261-b5fgb9wf	61	14	previously	previously	RB
cord-007261-b5fgb9wf	61	15	with	with	IN
cord-007261-b5fgb9wf	61	16	purified	purify	VBN
cord-007261-b5fgb9wf	61	17	rat	rat	NN
cord-007261-b5fgb9wf	61	18	liver	liver	NN
cord-007261-b5fgb9wf	61	19	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	61	20	carboxyesterase	carboxyesterase	NNP
cord-007261-b5fgb9wf	61	21	El	El	NNP
cord-007261-b5fgb9wf	61	22	as	as	IN
cord-007261-b5fgb9wf	61	23	the	the	DT
cord-007261-b5fgb9wf	61	24	antigen	antigen	NN
cord-007261-b5fgb9wf	61	25	(	(	-LRB-
cord-007261-b5fgb9wf	61	26	13	13	CD
cord-007261-b5fgb9wf	61	27	)	)	-RRB-
cord-007261-b5fgb9wf	61	28	.	.	.
cord-007261-b5fgb9wf	62	1	Anti	anti	JJ
cord-007261-b5fgb9wf	62	2	-	-	JJ
cord-007261-b5fgb9wf	62	3	Est	est	JJ
cord-007261-b5fgb9wf	62	4	antibodies	antibody	NNS
cord-007261-b5fgb9wf	62	5	recognize	recognize	VBP
cord-007261-b5fgb9wf	62	6	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	62	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	62	8	as	as	RB
cord-007261-b5fgb9wf	62	9	well	well	RB
cord-007261-b5fgb9wf	62	10	as	as	IN
cord-007261-b5fgb9wf	62	11	other	other	JJ
cord-007261-b5fgb9wf	62	12	microsomal	microsomal	JJ
cord-007261-b5fgb9wf	62	13	-	-	HYPH
cord-007261-b5fgb9wf	62	14	type	type	NN
cord-007261-b5fgb9wf	62	15	carboxyesterases	carboxyesterase	NNS
cord-007261-b5fgb9wf	62	16	,	,	,
cord-007261-b5fgb9wf	62	17	including	include	VBG
cord-007261-b5fgb9wf	62	18	carboxyesterase	carboxyesterase	NNP
cord-007261-b5fgb9wf	62	19	El	El	NNP
cord-007261-b5fgb9wf	62	20	.	.	.
cord-007261-b5fgb9wf	63	1	Rabbit	Rabbit	NNP
cord-007261-b5fgb9wf	63	2	anti	anti	JJ
cord-007261-b5fgb9wf	63	3	-	-	JJ
cord-007261-b5fgb9wf	63	4	P450(Ml	p450(ml	JJ
cord-007261-b5fgb9wf	63	5	)	)	-RRB-
cord-007261-b5fgb9wf	63	6	antibodies	antibody	NNS
cord-007261-b5fgb9wf	63	7	were	be	VBD
cord-007261-b5fgb9wf	63	8	previously	previously	RB
cord-007261-b5fgb9wf	63	9	described	describe	VBN
cord-007261-b5fgb9wf	63	10	(	(	-LRB-
cord-007261-b5fgb9wf	63	11	16	16	CD
cord-007261-b5fgb9wf	63	12	)	)	-RRB-
cord-007261-b5fgb9wf	63	13	.	.	.
cord-007261-b5fgb9wf	64	1	Rabbit	Rabbit	NNP
cord-007261-b5fgb9wf	64	2	anti	anti	JJ
cord-007261-b5fgb9wf	64	3	-	-	JJ
cord-007261-b5fgb9wf	64	4	cathepsin	cathepsin	JJ
cord-007261-b5fgb9wf	64	5	D	d	NN
cord-007261-b5fgb9wf	64	6	antibodies	antibody	NNS
cord-007261-b5fgb9wf	64	7	were	be	VBD
cord-007261-b5fgb9wf	64	8	a	a	DT
cord-007261-b5fgb9wf	64	9	gift	gift	NN
cord-007261-b5fgb9wf	64	10	from	from	IN
cord-007261-b5fgb9wf	64	11	Dr.	Dr.	NNP
cord-007261-b5fgb9wf	64	12	Himeno	Himeno	NNP
cord-007261-b5fgb9wf	64	13	of	of	IN
cord-007261-b5fgb9wf	64	14	Kyushu	Kyushu	NNP
cord-007261-b5fgb9wf	64	15	University	University	NNP
cord-007261-b5fgb9wf	64	16	.	.	.
cord-007261-b5fgb9wf	65	1	Transfections	Transfections	NNP
cord-007261-b5fgb9wf	65	2	,	,	,
cord-007261-b5fgb9wf	65	3	Labeling	labeling	NN
cord-007261-b5fgb9wf	65	4	,	,	,
cord-007261-b5fgb9wf	65	5	and	and	CC
cord-007261-b5fgb9wf	65	6	Immunoprecipitation	Immunoprecipitation	NNP
cord-007261-b5fgb9wf	65	7	-	-	HYPH
cord-007261-b5fgb9wf	65	8	COS-7	cos-7	NN
cord-007261-b5fgb9wf	65	9	cells	cell	NNS
cord-007261-b5fgb9wf	65	10	were	be	VBD
cord-007261-b5fgb9wf	65	11	seeded	seed	VBN
cord-007261-b5fgb9wf	65	12	on	on	IN
cord-007261-b5fgb9wf	65	13	the	the	DT
cord-007261-b5fgb9wf	65	14	day	day	NN
cord-007261-b5fgb9wf	65	15	before	before	IN
cord-007261-b5fgb9wf	65	16	the	the	DT
cord-007261-b5fgb9wf	65	17	transfection	transfection	NN
cord-007261-b5fgb9wf	65	18	at	at	IN
cord-007261-b5fgb9wf	65	19	a	a	DT
cord-007261-b5fgb9wf	65	20	density	density	NN
cord-007261-b5fgb9wf	65	21	of	of	IN
cord-007261-b5fgb9wf	65	22	1.2x10	1.2x10	CD
cord-007261-b5fgb9wf	65	23	*	*	NFP
cord-007261-b5fgb9wf	65	24	cells	cell	NNS
cord-007261-b5fgb9wf	65	25	per	per	IN
cord-007261-b5fgb9wf	65	26	10	10	CD
cord-007261-b5fgb9wf	65	27	cm	cm	NN
cord-007261-b5fgb9wf	65	28	culture	culture	NN
cord-007261-b5fgb9wf	65	29	dish	dish	NN
cord-007261-b5fgb9wf	65	30	in	in	IN
cord-007261-b5fgb9wf	65	31	Dulbecco	Dulbecco	NNP
cord-007261-b5fgb9wf	65	32	's	's	POS
cord-007261-b5fgb9wf	65	33	modified	modify	VBN
cord-007261-b5fgb9wf	65	34	Eagle	Eagle	NNP
cord-007261-b5fgb9wf	65	35	medium	medium	NN
cord-007261-b5fgb9wf	65	36	(	(	-LRB-
cord-007261-b5fgb9wf	65	37	GIBCO	GIBCO	NNP
cord-007261-b5fgb9wf	65	38	,	,	,
cord-007261-b5fgb9wf	65	39	Gent	Gent	NNP
cord-007261-b5fgb9wf	65	40	,	,	,
cord-007261-b5fgb9wf	65	41	Belgium	Belgium	NNP
cord-007261-b5fgb9wf	65	42	)	)	-RRB-
cord-007261-b5fgb9wf	65	43	,	,	,
cord-007261-b5fgb9wf	65	44	which	which	WDT
cord-007261-b5fgb9wf	65	45	contained	contain	VBD
cord-007261-b5fgb9wf	65	46	10	10	CD
cord-007261-b5fgb9wf	65	47	%	%	NN
cord-007261-b5fgb9wf	65	48	fetal	fetal	JJ
cord-007261-b5fgb9wf	65	49	calf	calf	NN
cord-007261-b5fgb9wf	65	50	serum	serum	NN
cord-007261-b5fgb9wf	65	51	.	.	.
cord-007261-b5fgb9wf	66	1	The	the	DT
cord-007261-b5fgb9wf	66	2	dish	dish	NN
cord-007261-b5fgb9wf	66	3	was	be	VBD
cord-007261-b5fgb9wf	66	4	incubated	incubate	VBN
cord-007261-b5fgb9wf	66	5	at	at	IN
cord-007261-b5fgb9wf	66	6	37"C	37"c	CD
cord-007261-b5fgb9wf	66	7	under	under	IN
cord-007261-b5fgb9wf	66	8	an	an	DT
cord-007261-b5fgb9wf	66	9	atmosphere	atmosphere	NN
cord-007261-b5fgb9wf	66	10	of	of	IN
cord-007261-b5fgb9wf	66	11	constant	constant	JJ
cord-007261-b5fgb9wf	66	12	humidity	humidity	NN
cord-007261-b5fgb9wf	66	13	(	(	-LRB-
cord-007261-b5fgb9wf	66	14	95	95	CD
cord-007261-b5fgb9wf	66	15	%	%	NN
cord-007261-b5fgb9wf	66	16	)	)	-RRB-
cord-007261-b5fgb9wf	66	17	comprising	comprise	VBG
cord-007261-b5fgb9wf	66	18	10	10	CD
cord-007261-b5fgb9wf	66	19	%	%	NN
cord-007261-b5fgb9wf	66	20	CO	co	NN
cord-007261-b5fgb9wf	66	21	2	2	CD
cord-007261-b5fgb9wf	66	22	in	in	IN
cord-007261-b5fgb9wf	66	23	air	air	NN
cord-007261-b5fgb9wf	66	24	.	.	.
cord-007261-b5fgb9wf	67	1	The	the	DT
cord-007261-b5fgb9wf	67	2	cells	cell	NNS
cord-007261-b5fgb9wf	67	3	were	be	VBD
cord-007261-b5fgb9wf	67	4	approximately	approximately	RB
cord-007261-b5fgb9wf	67	5	80	80	CD
cord-007261-b5fgb9wf	67	6	%	%	NN
cord-007261-b5fgb9wf	67	7	confluent	confluent	JJ
cord-007261-b5fgb9wf	67	8	after	after	IN
cord-007261-b5fgb9wf	67	9	24	24	CD
cord-007261-b5fgb9wf	67	10	h.	h.	NN
cord-007261-b5fgb9wf	68	1	The	the	DT
cord-007261-b5fgb9wf	68	2	plasmid	plasmid	NN
cord-007261-b5fgb9wf	68	3	DNA	dna	NN
cord-007261-b5fgb9wf	68	4	(	(	-LRB-
cord-007261-b5fgb9wf	68	5	30	30	CD
cord-007261-b5fgb9wf	68	6	pig	pig	NN
cord-007261-b5fgb9wf	68	7	)	)	-RRB-
cord-007261-b5fgb9wf	68	8	in	in	IN
cord-007261-b5fgb9wf	68	9	1.5	1.5	CD
cord-007261-b5fgb9wf	68	10	ml	ml	NNS
cord-007261-b5fgb9wf	68	11	of	of	IN
cord-007261-b5fgb9wf	68	12	phosphate	phosphate	NN
cord-007261-b5fgb9wf	68	13	-	-	HYPH
cord-007261-b5fgb9wf	68	14	buffered	buffer	VBN
cord-007261-b5fgb9wf	68	15	saline	saline	NN
cord-007261-b5fgb9wf	68	16	(	(	-LRB-
cord-007261-b5fgb9wf	68	17	PBS	PBS	NNP
cord-007261-b5fgb9wf	68	18	)	)	-RRB-
cord-007261-b5fgb9wf	68	19	was	be	VBD
cord-007261-b5fgb9wf	68	20	mixed	mix	VBN
cord-007261-b5fgb9wf	68	21	with	with	IN
cord-007261-b5fgb9wf	68	22	1.5	1.5	CD
cord-007261-b5fgb9wf	68	23	ml	ml	NNS
cord-007261-b5fgb9wf	68	24	of	of	IN
cord-007261-b5fgb9wf	68	25	a	a	DT
cord-007261-b5fgb9wf	68	26	cationic	cationic	JJ
cord-007261-b5fgb9wf	68	27	liposome	liposome	NN
cord-007261-b5fgb9wf	68	28	solution	solution	NN
cord-007261-b5fgb9wf	68	29	in	in	IN
cord-007261-b5fgb9wf	68	30	PBS	PBS	NNP
cord-007261-b5fgb9wf	68	31	(	(	-LRB-
cord-007261-b5fgb9wf	68	32	1	1	CD
cord-007261-b5fgb9wf	68	33	mg	mg	NNP
cord-007261-b5fgb9wf	68	34	/	/	SYM
cord-007261-b5fgb9wf	68	35	ml	ml	NNP
cord-007261-b5fgb9wf	68	36	)	)	-RRB-
cord-007261-b5fgb9wf	68	37	prepared	prepare	VBN
cord-007261-b5fgb9wf	68	38	with	with	IN
cord-007261-b5fgb9wf	68	39	0,0'didodecyl-	0,0'didodecyl-	NNP
cord-007261-b5fgb9wf	69	1	iV-[p-(2-trimethylarnmoniumethoxyl)benzoyl	iv-[p-(2-trimethylarnmoniumethoxyl)benzoyl	LS
cord-007261-b5fgb9wf	69	2	]	]	-RRB-
cord-007261-b5fgb9wf	70	1	-Lglutamate	-Lglutamate	JJR
cord-007261-b5fgb9wf	70	2	bromide	bromide	NN
cord-007261-b5fgb9wf	70	3	(	(	-LRB-
cord-007261-b5fgb9wf	70	4	17	17	CD
cord-007261-b5fgb9wf	70	5	)	)	-RRB-
cord-007261-b5fgb9wf	70	6	.	.	.
cord-007261-b5fgb9wf	71	1	The	the	DT
cord-007261-b5fgb9wf	71	2	DNA	dna	NN
cord-007261-b5fgb9wf	71	3	-	-	HYPH
cord-007261-b5fgb9wf	71	4	liposome	liposome	NN
cord-007261-b5fgb9wf	71	5	complex	complex	NN
cord-007261-b5fgb9wf	71	6	,	,	,
cord-007261-b5fgb9wf	71	7	which	which	WDT
cord-007261-b5fgb9wf	71	8	was	be	VBD
cord-007261-b5fgb9wf	71	9	formed	form	VBN
cord-007261-b5fgb9wf	71	10	by	by	IN
cord-007261-b5fgb9wf	71	11	mixing	mix	VBG
cord-007261-b5fgb9wf	71	12	,	,	,
cord-007261-b5fgb9wf	71	13	was	be	VBD
cord-007261-b5fgb9wf	71	14	added	add	VBN
cord-007261-b5fgb9wf	71	15	to	to	IN
cord-007261-b5fgb9wf	71	16	the	the	DT
cord-007261-b5fgb9wf	71	17	cell	cell	NN
cord-007261-b5fgb9wf	71	18	culture	culture	NN
cord-007261-b5fgb9wf	71	19	.	.	.
cord-007261-b5fgb9wf	72	1	The	the	DT
cord-007261-b5fgb9wf	72	2	cells	cell	NNS
cord-007261-b5fgb9wf	72	3	were	be	VBD
cord-007261-b5fgb9wf	72	4	incubated	incubate	VBN
cord-007261-b5fgb9wf	72	5	at	at	IN
cord-007261-b5fgb9wf	72	6	37*C	37*c	CD
cord-007261-b5fgb9wf	72	7	for	for	IN
cord-007261-b5fgb9wf	72	8	3	3	CD
cord-007261-b5fgb9wf	72	9	h	h	NN
cord-007261-b5fgb9wf	72	10	,	,	,
cord-007261-b5fgb9wf	72	11	and	and	CC
cord-007261-b5fgb9wf	72	12	then	then	RB
cord-007261-b5fgb9wf	72	13	10	10	CD
cord-007261-b5fgb9wf	72	14	ml	ml	NNS
cord-007261-b5fgb9wf	72	15	of	of	IN
cord-007261-b5fgb9wf	72	16	DMEM	DMEM	NNP
cord-007261-b5fgb9wf	72	17	supplemented	supplement	VBD
cord-007261-b5fgb9wf	72	18	with	with	IN
cord-007261-b5fgb9wf	72	19	10	10	CD
cord-007261-b5fgb9wf	72	20	%	%	NN
cord-007261-b5fgb9wf	72	21	fetal	fetal	JJ
cord-007261-b5fgb9wf	72	22	calf	calf	NN
cord-007261-b5fgb9wf	72	23	serum	serum	NN
cord-007261-b5fgb9wf	72	24	was	be	VBD
cord-007261-b5fgb9wf	72	25	added	add	VBN
cord-007261-b5fgb9wf	72	26	.	.	.
cord-007261-b5fgb9wf	73	1	Forty	forty	CD
cord-007261-b5fgb9wf	73	2	hours	hour	NNS
cord-007261-b5fgb9wf	73	3	later	later	RB
cord-007261-b5fgb9wf	73	4	,	,	,
cord-007261-b5fgb9wf	73	5	the	the	DT
cord-007261-b5fgb9wf	73	6	cells	cell	NNS
cord-007261-b5fgb9wf	73	7	were	be	VBD
cord-007261-b5fgb9wf	73	8	washed	wash	VBN
cord-007261-b5fgb9wf	73	9	with	with	IN
cord-007261-b5fgb9wf	73	10	a	a	DT
cord-007261-b5fgb9wf	73	11	methionine	methionine	NN
cord-007261-b5fgb9wf	73	12	-	-	HYPH
cord-007261-b5fgb9wf	73	13	free	free	JJ
cord-007261-b5fgb9wf	73	14	medium	medium	NN
cord-007261-b5fgb9wf	73	15	and	and	CC
cord-007261-b5fgb9wf	73	16	then	then	RB
cord-007261-b5fgb9wf	73	17	incubated	incubate	VBN
cord-007261-b5fgb9wf	73	18	in	in	IN
cord-007261-b5fgb9wf	73	19	the	the	DT
cord-007261-b5fgb9wf	73	20	same	same	JJ
cord-007261-b5fgb9wf	73	21	medium	medium	NN
cord-007261-b5fgb9wf	73	22	for	for	IN
cord-007261-b5fgb9wf	73	23	2	2	CD
cord-007261-b5fgb9wf	73	24	h.	h.	NN
cord-007261-b5fgb9wf	73	25	[	[	-LRB-
cord-007261-b5fgb9wf	73	26	36	36	CD
cord-007261-b5fgb9wf	73	27	S]Methionine	s]methionine	NN
cord-007261-b5fgb9wf	73	28	(	(	-LRB-
cord-007261-b5fgb9wf	73	29	200^Ci	200^ci	NN
cord-007261-b5fgb9wf	73	30	/	/	SYM
cord-007261-b5fgb9wf	73	31	ml	ml	NN
cord-007261-b5fgb9wf	73	32	)	)	-RRB-
cord-007261-b5fgb9wf	73	33	was	be	VBD
cord-007261-b5fgb9wf	73	34	then	then	RB
cord-007261-b5fgb9wf	73	35	added	add	VBN
cord-007261-b5fgb9wf	73	36	and	and	CC
cord-007261-b5fgb9wf	73	37	the	the	DT
cord-007261-b5fgb9wf	73	38	cells	cell	NNS
cord-007261-b5fgb9wf	73	39	were	be	VBD
cord-007261-b5fgb9wf	73	40	incubated	incubate	VBN
cord-007261-b5fgb9wf	73	41	for	for	IN
cord-007261-b5fgb9wf	73	42	3	3	CD
cord-007261-b5fgb9wf	73	43	h.	h.	NN
cord-007261-b5fgb9wf	74	1	For	for	IN
cord-007261-b5fgb9wf	74	2	pulse	pulse	NN
cord-007261-b5fgb9wf	74	3	and	and	CC
cord-007261-b5fgb9wf	74	4	chase	chase	NN
cord-007261-b5fgb9wf	74	5	studies	study	NNS
cord-007261-b5fgb9wf	74	6	,	,	,
cord-007261-b5fgb9wf	74	7	the	the	DT
cord-007261-b5fgb9wf	74	8	cells	cell	NNS
cord-007261-b5fgb9wf	74	9	were	be	VBD
cord-007261-b5fgb9wf	74	10	labeled	label	VBN
cord-007261-b5fgb9wf	74	11	as	as	IN
cord-007261-b5fgb9wf	74	12	above	above	RB
cord-007261-b5fgb9wf	74	13	,	,	,
cord-007261-b5fgb9wf	74	14	and	and	CC
cord-007261-b5fgb9wf	74	15	then	then	RB
cord-007261-b5fgb9wf	74	16	washed	wash	VBD
cord-007261-b5fgb9wf	74	17	with	with	IN
cord-007261-b5fgb9wf	74	18	fresh	fresh	JJ
cord-007261-b5fgb9wf	74	19	culture	culture	NN
cord-007261-b5fgb9wf	74	20	medium	medium	NN
cord-007261-b5fgb9wf	74	21	and	and	CC
cord-007261-b5fgb9wf	74	22	further	further	RB
cord-007261-b5fgb9wf	74	23	incubated	incubate	VBN
cord-007261-b5fgb9wf	74	24	in	in	IN
cord-007261-b5fgb9wf	74	25	fresh	fresh	JJ
cord-007261-b5fgb9wf	74	26	medium	medium	NN
cord-007261-b5fgb9wf	74	27	containing	contain	VBG
cord-007261-b5fgb9wf	74	28	2	2	CD
cord-007261-b5fgb9wf	74	29	mM	mm	CD
cord-007261-b5fgb9wf	74	30	non	non	JJ
cord-007261-b5fgb9wf	74	31	-	-	JJ
cord-007261-b5fgb9wf	74	32	radioactive	radioactive	JJ
cord-007261-b5fgb9wf	74	33	methionine	methionine	NN
cord-007261-b5fgb9wf	74	34	for	for	IN
cord-007261-b5fgb9wf	74	35	3	3	CD
cord-007261-b5fgb9wf	74	36	h.	h.	NN
cord-007261-b5fgb9wf	74	37	For	for	IN
cord-007261-b5fgb9wf	74	38	S2	S2	NNP
cord-007261-b5fgb9wf	74	39	P	P	NNP
cord-007261-b5fgb9wf	74	40	labeling	labeling	NN
cord-007261-b5fgb9wf	74	41	,	,	,
cord-007261-b5fgb9wf	74	42	the	the	DT
cord-007261-b5fgb9wf	74	43	cells	cell	NNS
cord-007261-b5fgb9wf	74	44	were	be	VBD
cord-007261-b5fgb9wf	74	45	pre	pre	JJ
cord-007261-b5fgb9wf	74	46	-	-	JJ
cord-007261-b5fgb9wf	74	47	incubated	incubate	VBN
cord-007261-b5fgb9wf	74	48	for	for	IN
cord-007261-b5fgb9wf	74	49	1	1	CD
cord-007261-b5fgb9wf	74	50	h	h	NN
cord-007261-b5fgb9wf	74	51	in	in	IN
cord-007261-b5fgb9wf	74	52	a	a	DT
cord-007261-b5fgb9wf	74	53	phosphate	phosphate	NN
cord-007261-b5fgb9wf	74	54	-	-	HYPH
cord-007261-b5fgb9wf	74	55	free	free	JJ
cord-007261-b5fgb9wf	74	56	medium	medium	NN
cord-007261-b5fgb9wf	74	57	,	,	,
cord-007261-b5fgb9wf	74	58	and	and	CC
cord-007261-b5fgb9wf	74	59	then	then	RB
cord-007261-b5fgb9wf	74	60	labeled	label	VBN
cord-007261-b5fgb9wf	74	61	in	in	IN
cord-007261-b5fgb9wf	74	62	the	the	DT
cord-007261-b5fgb9wf	74	63	same	same	JJ
cord-007261-b5fgb9wf	74	64	medium	medium	NN
cord-007261-b5fgb9wf	74	65	with	with	IN
cord-007261-b5fgb9wf	74	66	1	1	CD
cord-007261-b5fgb9wf	74	67	mCi	mCi	NNS
cord-007261-b5fgb9wf	74	68	32	32	CD
cord-007261-b5fgb9wf	74	69	PO	po	NN
cord-007261-b5fgb9wf	74	70	4	4	CD
cord-007261-b5fgb9wf	74	71	per	per	IN
cord-007261-b5fgb9wf	74	72	dish	dish	NN
cord-007261-b5fgb9wf	74	73	for	for	IN
cord-007261-b5fgb9wf	74	74	2	2	CD
cord-007261-b5fgb9wf	74	75	h.	h.	NN
cord-007261-b5fgb9wf	75	1	For	for	IN
cord-007261-b5fgb9wf	75	2	immunoprecipitation	immunoprecipitation	NN
cord-007261-b5fgb9wf	75	3	,	,	,
cord-007261-b5fgb9wf	75	4	the	the	DT
cord-007261-b5fgb9wf	75	5	culture	culture	NN
cord-007261-b5fgb9wf	75	6	medium	medium	NN
cord-007261-b5fgb9wf	75	7	was	be	VBD
cord-007261-b5fgb9wf	75	8	separated	separate	VBN
cord-007261-b5fgb9wf	75	9	from	from	IN
cord-007261-b5fgb9wf	75	10	the	the	DT
cord-007261-b5fgb9wf	75	11	cells	cell	NNS
cord-007261-b5fgb9wf	75	12	by	by	IN
cord-007261-b5fgb9wf	75	13	decantation	decantation	NN
cord-007261-b5fgb9wf	75	14	.	.	.
cord-007261-b5fgb9wf	76	1	The	the	DT
cord-007261-b5fgb9wf	76	2	cells	cell	NNS
cord-007261-b5fgb9wf	76	3	were	be	VBD
cord-007261-b5fgb9wf	76	4	washed	wash	VBN
cord-007261-b5fgb9wf	76	5	with	with	IN
cord-007261-b5fgb9wf	76	6	PBS	PBS	NNP
cord-007261-b5fgb9wf	76	7	,	,	,
cord-007261-b5fgb9wf	76	8	and	and	CC
cord-007261-b5fgb9wf	76	9	then	then	RB
cord-007261-b5fgb9wf	76	10	scraped	scrape	VBD
cord-007261-b5fgb9wf	76	11	off	off	RP
cord-007261-b5fgb9wf	76	12	with	with	IN
cord-007261-b5fgb9wf	76	13	a	a	DT
cord-007261-b5fgb9wf	76	14	rubber	rubber	NN
cord-007261-b5fgb9wf	76	15	policeman	policeman	NN
cord-007261-b5fgb9wf	76	16	into	into	IN
cord-007261-b5fgb9wf	76	17	1	1	CD
cord-007261-b5fgb9wf	76	18	ml	ml	NNS
cord-007261-b5fgb9wf	76	19	of	of	IN
cord-007261-b5fgb9wf	76	20	PBS	PBS	NNP
cord-007261-b5fgb9wf	76	21	.	.	.
cord-007261-b5fgb9wf	77	1	A	a	DT
cord-007261-b5fgb9wf	77	2	0.1	0.1	CD
cord-007261-b5fgb9wf	77	3	volume	volume	NN
cord-007261-b5fgb9wf	77	4	of	of	IN
cord-007261-b5fgb9wf	77	5	10	10	CD
cord-007261-b5fgb9wf	77	6	%	%	NN
cord-007261-b5fgb9wf	77	7	SDS	SDS	NNP
cord-007261-b5fgb9wf	77	8	was	be	VBD
cord-007261-b5fgb9wf	77	9	added	add	VBN
cord-007261-b5fgb9wf	77	10	to	to	IN
cord-007261-b5fgb9wf	77	11	the	the	DT
cord-007261-b5fgb9wf	77	12	culture	culture	NN
cord-007261-b5fgb9wf	77	13	medium	medium	NN
cord-007261-b5fgb9wf	77	14	,	,	,
cord-007261-b5fgb9wf	77	15	and	and	CC
cord-007261-b5fgb9wf	77	16	the	the	DT
cord-007261-b5fgb9wf	77	17	mixture	mixture	NN
cord-007261-b5fgb9wf	77	18	was	be	VBD
cord-007261-b5fgb9wf	77	19	heated	heat	VBN
cord-007261-b5fgb9wf	77	20	at	at	IN
cord-007261-b5fgb9wf	77	21	100'C	100'c	CD
cord-007261-b5fgb9wf	77	22	for	for	IN
cord-007261-b5fgb9wf	77	23	5	5	CD
cord-007261-b5fgb9wf	77	24	min	min	NN
cord-007261-b5fgb9wf	77	25	.	.	.
cord-007261-b5fgb9wf	78	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	78	2	was	be	VBD
cord-007261-b5fgb9wf	78	3	then	then	RB
cord-007261-b5fgb9wf	78	4	diluted	dilute	VBN
cord-007261-b5fgb9wf	78	5	with	with	IN
cord-007261-b5fgb9wf	78	6	9	9	CD
cord-007261-b5fgb9wf	78	7	volumes	volume	NNS
cord-007261-b5fgb9wf	78	8	of	of	IN
cord-007261-b5fgb9wf	78	9	dilution	dilution	NN
cord-007261-b5fgb9wf	78	10	buffer	buffer	NN
cord-007261-b5fgb9wf	78	11	[	[	-LRB-
cord-007261-b5fgb9wf	78	12	0.5	0.5	CD
cord-007261-b5fgb9wf	78	13	%	%	NN
cord-007261-b5fgb9wf	78	14	Triton	Triton	NNP
cord-007261-b5fgb9wf	78	15	X-100	x-100	NN
cord-007261-b5fgb9wf	78	16	,	,	,
cord-007261-b5fgb9wf	78	17	150	150	CD
cord-007261-b5fgb9wf	78	18	mM	mm	CD
cord-007261-b5fgb9wf	78	19	NaCl	nacl	NN
cord-007261-b5fgb9wf	78	20	,	,	,
cord-007261-b5fgb9wf	78	21	50	50	CD
cord-007261-b5fgb9wf	78	22	mM	mm	CD
cord-007261-b5fgb9wf	78	23	Tris	Tris	NNP
cord-007261-b5fgb9wf	78	24	-	-	HYPH
cord-007261-b5fgb9wf	78	25	HCl	HCl	NNP
cord-007261-b5fgb9wf	78	26	(	(	-LRB-
cord-007261-b5fgb9wf	78	27	pH	pH	NNP
cord-007261-b5fgb9wf	78	28	7.4	7.4	CD
cord-007261-b5fgb9wf	78	29	)	)	-RRB-
cord-007261-b5fgb9wf	78	30	,	,	,
cord-007261-b5fgb9wf	78	31	and	and	CC
cord-007261-b5fgb9wf	78	32	5	5	CD
cord-007261-b5fgb9wf	78	33	mM	mm	CD
cord-007261-b5fgb9wf	78	34	EDTA	edta	NN
cord-007261-b5fgb9wf	78	35	}	}	-RRB-
cord-007261-b5fgb9wf	78	36	.	.	.
cord-007261-b5fgb9wf	79	1	The	the	DT
cord-007261-b5fgb9wf	79	2	cells	cell	NNS
cord-007261-b5fgb9wf	79	3	were	be	VBD
cord-007261-b5fgb9wf	79	4	homogenized	homogenize	VBN
cord-007261-b5fgb9wf	79	5	with	with	IN
cord-007261-b5fgb9wf	79	6	1	1	CD
cord-007261-b5fgb9wf	79	7	ml	ml	NNS
cord-007261-b5fgb9wf	79	8	of	of	IN
cord-007261-b5fgb9wf	79	9	PBS	PBS	NNP
cord-007261-b5fgb9wf	79	10	containing	contain	VBG
cord-007261-b5fgb9wf	79	11	1	1	CD
cord-007261-b5fgb9wf	79	12	%	%	NN
cord-007261-b5fgb9wf	79	13	SDS	SDS	NNP
cord-007261-b5fgb9wf	79	14	by	by	IN
cord-007261-b5fgb9wf	79	15	50	50	CD
cord-007261-b5fgb9wf	79	16	strokes	stroke	NNS
cord-007261-b5fgb9wf	79	17	through	through	IN
cord-007261-b5fgb9wf	79	18	a	a	DT
cord-007261-b5fgb9wf	79	19	syringe	syringe	NN
cord-007261-b5fgb9wf	79	20	needle	needle	NN
cord-007261-b5fgb9wf	79	21	,	,	,
cord-007261-b5fgb9wf	79	22	and	and	CC
cord-007261-b5fgb9wf	79	23	then	then	RB
cord-007261-b5fgb9wf	79	24	diluted	dilute	VBN
cord-007261-b5fgb9wf	79	25	with	with	IN
cord-007261-b5fgb9wf	79	26	9	9	CD
cord-007261-b5fgb9wf	79	27	volumes	volume	NNS
cord-007261-b5fgb9wf	79	28	of	of	IN
cord-007261-b5fgb9wf	79	29	the	the	DT
cord-007261-b5fgb9wf	79	30	dilution	dilution	NN
cord-007261-b5fgb9wf	79	31	buffer	buffer	NN
cord-007261-b5fgb9wf	79	32	.	.	.
cord-007261-b5fgb9wf	80	1	Equivalent	equivalent	JJ
cord-007261-b5fgb9wf	80	2	amounts	amount	NNS
cord-007261-b5fgb9wf	80	3	of	of	IN
cord-007261-b5fgb9wf	80	4	the	the	DT
cord-007261-b5fgb9wf	80	5	culture	culture	NN
cord-007261-b5fgb9wf	80	6	medium	medium	NN
cord-007261-b5fgb9wf	80	7	and	and	CC
cord-007261-b5fgb9wf	80	8	cell	cell	NN
cord-007261-b5fgb9wf	80	9	extracts	extract	NNS
cord-007261-b5fgb9wf	80	10	were	be	VBD
cord-007261-b5fgb9wf	80	11	incubated	incubate	VBN
cord-007261-b5fgb9wf	80	12	with	with	IN
cord-007261-b5fgb9wf	80	13	the	the	DT
cord-007261-b5fgb9wf	80	14	appropriate	appropriate	JJ
cord-007261-b5fgb9wf	80	15	antibodies	antibody	NNS
cord-007261-b5fgb9wf	80	16	at	at	IN
cord-007261-b5fgb9wf	80	17	4'C	4'c	CD
cord-007261-b5fgb9wf	80	18	for	for	IN
cord-007261-b5fgb9wf	80	19	1	1	CD
cord-007261-b5fgb9wf	80	20	h.	h.	NN
cord-007261-b5fgb9wf	81	1	The	the	DT
cord-007261-b5fgb9wf	81	2	immunocomplexes	immunocomplexe	NNS
cord-007261-b5fgb9wf	81	3	were	be	VBD
cord-007261-b5fgb9wf	81	4	recovered	recover	VBN
cord-007261-b5fgb9wf	81	5	using	use	VBG
cord-007261-b5fgb9wf	81	6	Protein	Protein	NNP
cord-007261-b5fgb9wf	81	7	A	A	NNP
cord-007261-b5fgb9wf	81	8	-	-	:
cord-007261-b5fgb9wf	81	9	Sepharose	Sepharose	NNP
cord-007261-b5fgb9wf	81	10	as	as	IN
cord-007261-b5fgb9wf	81	11	described	describe	VBN
cord-007261-b5fgb9wf	81	12	previously	previously	RB
cord-007261-b5fgb9wf	81	13	(	(	-LRB-
cord-007261-b5fgb9wf	81	14	18	18	CD
cord-007261-b5fgb9wf	81	15	)	)	-RRB-
cord-007261-b5fgb9wf	81	16	.	.	.
cord-007261-b5fgb9wf	82	1	Cell	cell	NN
cord-007261-b5fgb9wf	82	2	Fractionation	fractionation	NN
cord-007261-b5fgb9wf	82	3	and	and	CC
cord-007261-b5fgb9wf	82	4	Alkali	Alkali	NNP
cord-007261-b5fgb9wf	82	5	Extraction	Extraction	NNP
cord-007261-b5fgb9wf	82	6	of	of	IN
cord-007261-b5fgb9wf	82	7	the	the	DT
cord-007261-b5fgb9wf	82	8	Membrane	Membrane	NNP
cord-007261-b5fgb9wf	82	9	Fraction	Fraction	NNP
cord-007261-b5fgb9wf	82	10	-	-	HYPH
cord-007261-b5fgb9wf	82	11	Cells	cell	NNS
cord-007261-b5fgb9wf	82	12	were	be	VBD
cord-007261-b5fgb9wf	82	13	washed	wash	VBN
cord-007261-b5fgb9wf	82	14	with	with	IN
cord-007261-b5fgb9wf	82	15	PBS	PBS	NNP
cord-007261-b5fgb9wf	82	16	and	and	CC
cord-007261-b5fgb9wf	82	17	harvested	harvest	VBN
cord-007261-b5fgb9wf	82	18	in	in	IN
cord-007261-b5fgb9wf	82	19	ice	ice	NN
cord-007261-b5fgb9wf	82	20	-	-	HYPH
cord-007261-b5fgb9wf	82	21	cold	cold	JJ
cord-007261-b5fgb9wf	82	22	STE	STE	NNP
cord-007261-b5fgb9wf	82	23	buffer	buffer	NN
cord-007261-b5fgb9wf	82	24	[	[	-LRB-
cord-007261-b5fgb9wf	82	25	0.25	0.25	CD
cord-007261-b5fgb9wf	82	26	M	M	NNP
cord-007261-b5fgb9wf	82	27	sucrose	sucrose	NN
cord-007261-b5fgb9wf	82	28	,	,	,
cord-007261-b5fgb9wf	82	29	20	20	CD
cord-007261-b5fgb9wf	82	30	mM	mM	NNP
cord-007261-b5fgb9wf	82	31	Tris	Tris	NNP
cord-007261-b5fgb9wf	82	32	-	-	HYPH
cord-007261-b5fgb9wf	82	33	HCl	HCl	NNP
cord-007261-b5fgb9wf	82	34	buffer	buffer	NN
cord-007261-b5fgb9wf	82	35	(	(	-LRB-
cord-007261-b5fgb9wf	82	36	pH	pH	NNP
cord-007261-b5fgb9wf	82	37	8.0	8.0	CD
cord-007261-b5fgb9wf	82	38	)	)	-RRB-
cord-007261-b5fgb9wf	82	39	,	,	,
cord-007261-b5fgb9wf	82	40	1	1	CD
cord-007261-b5fgb9wf	82	41	mM	mm	CD
cord-007261-b5fgb9wf	82	42	EDTA	EDTA	NNP
cord-007261-b5fgb9wf	82	43	,	,	,
cord-007261-b5fgb9wf	82	44	and	and	CC
cord-007261-b5fgb9wf	82	45	2	2	CD
cord-007261-b5fgb9wf	82	46	//g	//g	NNS
cord-007261-b5fgb9wf	82	47	/	/	SYM
cord-007261-b5fgb9wf	82	48	ml	ml	IN
cord-007261-b5fgb9wf	82	49	each	each	DT
cord-007261-b5fgb9wf	82	50	of	of	IN
cord-007261-b5fgb9wf	82	51	leupeptin	leupeptin	NN
cord-007261-b5fgb9wf	82	52	and	and	CC
cord-007261-b5fgb9wf	82	53	pepstatin	pepstatin	NNP
cord-007261-b5fgb9wf	82	54	]	]	-RRB-
cord-007261-b5fgb9wf	82	55	.	.	.
cord-007261-b5fgb9wf	83	1	The	the	DT
cord-007261-b5fgb9wf	83	2	suspension	suspension	NN
cord-007261-b5fgb9wf	83	3	was	be	VBD
cord-007261-b5fgb9wf	83	4	centrifuged	centrifuge	VBN
cord-007261-b5fgb9wf	83	5	at	at	IN
cord-007261-b5fgb9wf	83	6	500	500	CD
cord-007261-b5fgb9wf	83	7	X	x	NN
cord-007261-b5fgb9wf	83	8	g	g	NN
cord-007261-b5fgb9wf	83	9	for	for	IN
cord-007261-b5fgb9wf	83	10	5	5	CD
cord-007261-b5fgb9wf	83	11	min	min	NN
cord-007261-b5fgb9wf	83	12	and	and	CC
cord-007261-b5fgb9wf	83	13	the	the	DT
cord-007261-b5fgb9wf	83	14	pellet	pellet	NN
cord-007261-b5fgb9wf	83	15	was	be	VBD
cord-007261-b5fgb9wf	83	16	suspended	suspend	VBN
cord-007261-b5fgb9wf	83	17	in	in	IN
cord-007261-b5fgb9wf	83	18	a	a	DT
cord-007261-b5fgb9wf	83	19	hypotonic	hypotonic	JJ
cord-007261-b5fgb9wf	83	20	buffer	buffer	NN
cord-007261-b5fgb9wf	83	21	[	[	-LRB-
cord-007261-b5fgb9wf	83	22	0.025	0.025	CD
cord-007261-b5fgb9wf	83	23	M	M	NNP
cord-007261-b5fgb9wf	83	24	sucrose	sucrose	NN
cord-007261-b5fgb9wf	83	25	,	,	,
cord-007261-b5fgb9wf	83	26	20	20	CD
cord-007261-b5fgb9wf	83	27	mM	mM	NNP
cord-007261-b5fgb9wf	83	28	Tris	Tris	NNP
cord-007261-b5fgb9wf	83	29	-	-	HYPH
cord-007261-b5fgb9wf	83	30	HCl	HCl	NNP
cord-007261-b5fgb9wf	83	31	buffer	buffer	NN
cord-007261-b5fgb9wf	83	32	(	(	-LRB-
cord-007261-b5fgb9wf	83	33	pH	pH	NNP
cord-007261-b5fgb9wf	83	34	8.0	8.0	CD
cord-007261-b5fgb9wf	83	35	)	)	-RRB-
cord-007261-b5fgb9wf	83	36	,	,	,
cord-007261-b5fgb9wf	83	37	1	1	CD
cord-007261-b5fgb9wf	83	38	mM	mm	CD
cord-007261-b5fgb9wf	83	39	EDTA	EDTA	NNP
cord-007261-b5fgb9wf	83	40	,	,	,
cord-007261-b5fgb9wf	83	41	and	and	CC
cord-007261-b5fgb9wf	83	42	2	2	CD
cord-007261-b5fgb9wf	83	43	//g	//g	NNS
cord-007261-b5fgb9wf	83	44	/	/	SYM
cord-007261-b5fgb9wf	83	45	ml	ml	IN
cord-007261-b5fgb9wf	83	46	each	each	DT
cord-007261-b5fgb9wf	83	47	of	of	IN
cord-007261-b5fgb9wf	83	48	leupeptin	leupeptin	NN
cord-007261-b5fgb9wf	83	49	and	and	CC
cord-007261-b5fgb9wf	83	50	pepstatin	pepstatin	NNP
cord-007261-b5fgb9wf	83	51	]	]	-RRB-
cord-007261-b5fgb9wf	83	52	.	.	.
cord-007261-b5fgb9wf	84	1	The	the	DT
cord-007261-b5fgb9wf	84	2	suspension	suspension	NN
cord-007261-b5fgb9wf	84	3	was	be	VBD
cord-007261-b5fgb9wf	84	4	kept	keep	VBN
cord-007261-b5fgb9wf	84	5	on	on	IN
cord-007261-b5fgb9wf	84	6	ice	ice	NN
cord-007261-b5fgb9wf	84	7	for	for	IN
cord-007261-b5fgb9wf	84	8	30	30	CD
cord-007261-b5fgb9wf	84	9	min	min	NN
cord-007261-b5fgb9wf	84	10	and	and	CC
cord-007261-b5fgb9wf	84	11	then	then	RB
cord-007261-b5fgb9wf	84	12	centrifuged	centrifuge	VBN
cord-007261-b5fgb9wf	84	13	.	.	.
cord-007261-b5fgb9wf	85	1	The	the	DT
cord-007261-b5fgb9wf	85	2	precipitated	precipitated	JJ
cord-007261-b5fgb9wf	85	3	cells	cell	NNS
cord-007261-b5fgb9wf	85	4	were	be	VBD
cord-007261-b5fgb9wf	85	5	suspended	suspend	VBN
cord-007261-b5fgb9wf	85	6	in	in	IN
cord-007261-b5fgb9wf	85	7	1	1	CD
cord-007261-b5fgb9wf	85	8	ml	ml	NNS
cord-007261-b5fgb9wf	85	9	of	of	IN
cord-007261-b5fgb9wf	85	10	STE	STE	NNP
cord-007261-b5fgb9wf	85	11	buffer	buffer	NN
cord-007261-b5fgb9wf	85	12	and	and	CC
cord-007261-b5fgb9wf	85	13	then	then	RB
cord-007261-b5fgb9wf	85	14	homogenized	homogenize	VBN
cord-007261-b5fgb9wf	85	15	with	with	IN
cord-007261-b5fgb9wf	85	16	a	a	DT
cord-007261-b5fgb9wf	85	17	Dounce	Dounce	NNP
cord-007261-b5fgb9wf	85	18	homogenizer	homogenizer	NN
cord-007261-b5fgb9wf	85	19	.	.	.
cord-007261-b5fgb9wf	86	1	The	the	DT
cord-007261-b5fgb9wf	86	2	homogenate	homogenate	NNP
cord-007261-b5fgb9wf	86	3	was	be	VBD
cord-007261-b5fgb9wf	86	4	centrifuged	centrifuge	VBN
cord-007261-b5fgb9wf	86	5	at	at	IN
cord-007261-b5fgb9wf	86	6	500	500	CD
cord-007261-b5fgb9wf	86	7	X	x	NN
cord-007261-b5fgb9wf	86	8	g	g	NN
cord-007261-b5fgb9wf	86	9	for	for	IN
cord-007261-b5fgb9wf	86	10	5	5	CD
cord-007261-b5fgb9wf	86	11	min	min	NN
cord-007261-b5fgb9wf	86	12	to	to	TO
cord-007261-b5fgb9wf	86	13	precipitate	precipitate	VB
cord-007261-b5fgb9wf	86	14	the	the	DT
cord-007261-b5fgb9wf	86	15	nuclei	nucleus	NNS
cord-007261-b5fgb9wf	86	16	and	and	CC
cord-007261-b5fgb9wf	86	17	undisrupted	undisrupted	JJ
cord-007261-b5fgb9wf	86	18	cells	cell	NNS
cord-007261-b5fgb9wf	86	19	.	.	.
cord-007261-b5fgb9wf	87	1	The	the	DT
cord-007261-b5fgb9wf	87	2	supernatant	supernatant	JJ
cord-007261-b5fgb9wf	87	3	(	(	-LRB-
cord-007261-b5fgb9wf	87	4	crude	crude	JJ
cord-007261-b5fgb9wf	87	5	membrane	membrane	NN
cord-007261-b5fgb9wf	87	6	fraction	fraction	NN
cord-007261-b5fgb9wf	87	7	)	)	-RRB-
cord-007261-b5fgb9wf	87	8	was	be	VBD
cord-007261-b5fgb9wf	87	9	layered	layer	VBN
cord-007261-b5fgb9wf	87	10	on	on	IN
cord-007261-b5fgb9wf	87	11	a	a	DT
cord-007261-b5fgb9wf	87	12	discontinuous	discontinuous	JJ
cord-007261-b5fgb9wf	87	13	sucrose	sucrose	NN
cord-007261-b5fgb9wf	87	14	gradient	gradient	NN
cord-007261-b5fgb9wf	87	15	consisting	consist	VBG
cord-007261-b5fgb9wf	87	16	of	of	IN
cord-007261-b5fgb9wf	87	17	1	1	CD
cord-007261-b5fgb9wf	87	18	ml	ml	NNS
cord-007261-b5fgb9wf	87	19	of	of	IN
cord-007261-b5fgb9wf	87	20	2	2	CD
cord-007261-b5fgb9wf	87	21	M	M	NNP
cord-007261-b5fgb9wf	87	22	sucrose	sucrose	NN
cord-007261-b5fgb9wf	87	23	,	,	,
cord-007261-b5fgb9wf	87	24	3.4	3.4	CD
cord-007261-b5fgb9wf	87	25	ml	ml	NNS
cord-007261-b5fgb9wf	87	26	of	of	IN
cord-007261-b5fgb9wf	87	27	1.3	1.3	CD
cord-007261-b5fgb9wf	87	28	M	M	NNP
cord-007261-b5fgb9wf	87	29	sucrose	sucrose	NN
cord-007261-b5fgb9wf	87	30	,	,	,
cord-007261-b5fgb9wf	87	31	3.4	3.4	CD
cord-007261-b5fgb9wf	87	32	ml	ml	NNS
cord-007261-b5fgb9wf	87	33	of	of	IN
cord-007261-b5fgb9wf	87	34	1	1	CD
cord-007261-b5fgb9wf	87	35	M	M	NNP
cord-007261-b5fgb9wf	87	36	sucrose	sucrose	NN
cord-007261-b5fgb9wf	87	37	,	,	,
cord-007261-b5fgb9wf	87	38	and	and	CC
cord-007261-b5fgb9wf	87	39	2.75	2.75	CD
cord-007261-b5fgb9wf	87	40	ml	ml	NNS
cord-007261-b5fgb9wf	87	41	of	of	IN
cord-007261-b5fgb9wf	87	42	0.6	0.6	CD
cord-007261-b5fgb9wf	87	43	M	M	NNP
cord-007261-b5fgb9wf	87	44	sucrose	sucrose	NN
cord-007261-b5fgb9wf	87	45	(	(	-LRB-
cord-007261-b5fgb9wf	87	46	19	19	CD
cord-007261-b5fgb9wf	87	47	)	)	-RRB-
cord-007261-b5fgb9wf	87	48	,	,	,
cord-007261-b5fgb9wf	87	49	and	and	CC
cord-007261-b5fgb9wf	87	50	then	then	RB
cord-007261-b5fgb9wf	87	51	centrifuged	centrifuge	VBD
cord-007261-b5fgb9wf	87	52	for	for	IN
cord-007261-b5fgb9wf	87	53	4	4	CD
cord-007261-b5fgb9wf	87	54	h	h	NN
cord-007261-b5fgb9wf	87	55	at	at	IN
cord-007261-b5fgb9wf	87	56	40,000	40,000	CD
cord-007261-b5fgb9wf	87	57	rpm	rpm	NN
cord-007261-b5fgb9wf	87	58	in	in	IN
cord-007261-b5fgb9wf	87	59	an	an	DT
cord-007261-b5fgb9wf	87	60	RPS-40	RPS-40	NNP
cord-007261-b5fgb9wf	87	61	T	t	NN
cord-007261-b5fgb9wf	87	62	rotor	rotor	NN
cord-007261-b5fgb9wf	87	63	(	(	-LRB-
cord-007261-b5fgb9wf	87	64	Hitachi	Hitachi	NNP
cord-007261-b5fgb9wf	87	65	)	)	-RRB-
cord-007261-b5fgb9wf	87	66	.	.	.
cord-007261-b5fgb9wf	88	1	Fractions	fraction	NNS
cord-007261-b5fgb9wf	88	2	were	be	VBD
cord-007261-b5fgb9wf	88	3	collected	collect	VBN
cord-007261-b5fgb9wf	88	4	from	from	IN
cord-007261-b5fgb9wf	88	5	the	the	DT
cord-007261-b5fgb9wf	88	6	top	top	NN
cord-007261-b5fgb9wf	88	7	of	of	IN
cord-007261-b5fgb9wf	88	8	the	the	DT
cord-007261-b5fgb9wf	88	9	tube	tube	NN
cord-007261-b5fgb9wf	88	10	.	.	.
cord-007261-b5fgb9wf	89	1	For	for	IN
cord-007261-b5fgb9wf	89	2	alkali	alkali	JJ
cord-007261-b5fgb9wf	89	3	extraction	extraction	NN
cord-007261-b5fgb9wf	89	4	,	,	,
cord-007261-b5fgb9wf	89	5	the	the	DT
cord-007261-b5fgb9wf	89	6	crude	crude	JJ
cord-007261-b5fgb9wf	89	7	membrane	membrane	NN
cord-007261-b5fgb9wf	89	8	fraction	fraction	NN
cord-007261-b5fgb9wf	89	9	was	be	VBD
cord-007261-b5fgb9wf	89	10	treated	treat	VBN
cord-007261-b5fgb9wf	89	11	with	with	IN
cord-007261-b5fgb9wf	89	12	100	100	CD
cord-007261-b5fgb9wf	89	13	mM	mm	CD
cord-007261-b5fgb9wf	89	14	Na	Na	NNP
cord-007261-b5fgb9wf	89	15	2	2	CD
cord-007261-b5fgb9wf	89	16	CO	CO	NNP
cord-007261-b5fgb9wf	89	17	3	3	CD
cord-007261-b5fgb9wf	89	18	on	on	IN
cord-007261-b5fgb9wf	89	19	ice	ice	NN
cord-007261-b5fgb9wf	89	20	for	for	IN
cord-007261-b5fgb9wf	89	21	30	30	CD
cord-007261-b5fgb9wf	89	22	min	min	NN
cord-007261-b5fgb9wf	89	23	(	(	-LRB-
cord-007261-b5fgb9wf	89	24	20	20	CD
cord-007261-b5fgb9wf	89	25	)	)	-RRB-
cord-007261-b5fgb9wf	89	26	.	.	.
cord-007261-b5fgb9wf	90	1	Western	Western	NNP
cord-007261-b5fgb9wf	90	2	Blotting	Blotting	NNP
cord-007261-b5fgb9wf	90	3	-	-	HYPH
cord-007261-b5fgb9wf	90	4	SDS	SDS	NNP
cord-007261-b5fgb9wf	90	5	-	-	HYPH
cord-007261-b5fgb9wf	90	6	PAGE	PAGE	NNP
cord-007261-b5fgb9wf	90	7	was	be	VBD
cord-007261-b5fgb9wf	90	8	carried	carry	VBN
cord-007261-b5fgb9wf	90	9	out	out	RP
cord-007261-b5fgb9wf	90	10	using	use	VBG
cord-007261-b5fgb9wf	90	11	10	10	CD
cord-007261-b5fgb9wf	90	12	%	%	NN
cord-007261-b5fgb9wf	90	13	polyacrylamide	polyacrylamide	NN
cord-007261-b5fgb9wf	90	14	gels	gel	NNS
cord-007261-b5fgb9wf	90	15	containing	contain	VBG
cord-007261-b5fgb9wf	90	16	0.1	0.1	CD
cord-007261-b5fgb9wf	90	17	%	%	NN
cord-007261-b5fgb9wf	90	18	SDS	SDS	NNP
cord-007261-b5fgb9wf	90	19	.	.	.
cord-007261-b5fgb9wf	91	1	The	the	DT
cord-007261-b5fgb9wf	91	2	gels	gel	NNS
cord-007261-b5fgb9wf	91	3	were	be	VBD
cord-007261-b5fgb9wf	91	4	blotted	blot	VBN
cord-007261-b5fgb9wf	91	5	onto	onto	IN
cord-007261-b5fgb9wf	91	6	nitrocellulose	nitrocellulose	JJ
cord-007261-b5fgb9wf	91	7	niters	niter	NNS
cord-007261-b5fgb9wf	91	8	as	as	IN
cord-007261-b5fgb9wf	91	9	described	describe	VBN
cord-007261-b5fgb9wf	91	10	by	by	IN
cord-007261-b5fgb9wf	91	11	Towbin	Towbin	NNP
cord-007261-b5fgb9wf	91	12	et	et	NNP
cord-007261-b5fgb9wf	91	13	al	al	NNP
cord-007261-b5fgb9wf	91	14	.	.	.
cord-007261-b5fgb9wf	92	1	(	(	-LRB-
cord-007261-b5fgb9wf	92	2	21	21	CD
cord-007261-b5fgb9wf	92	3	)	)	-RRB-
cord-007261-b5fgb9wf	92	4	,	,	.
cord-007261-b5fgb9wf	93	1	and	and	CC
cord-007261-b5fgb9wf	93	2	the	the	DT
cord-007261-b5fgb9wf	93	3	filters	filter	NNS
cord-007261-b5fgb9wf	93	4	were	be	VBD
cord-007261-b5fgb9wf	93	5	incubated	incubate	VBN
cord-007261-b5fgb9wf	93	6	in	in	IN
cord-007261-b5fgb9wf	93	7	PBS	PBS	NNP
cord-007261-b5fgb9wf	93	8	containing	contain	VBG
cord-007261-b5fgb9wf	93	9	1	1	CD
cord-007261-b5fgb9wf	93	10	%	%	NN
cord-007261-b5fgb9wf	93	11	skim	skim	NN
cord-007261-b5fgb9wf	93	12	milk	milk	NN
cord-007261-b5fgb9wf	93	13	powder	powder	NN
cord-007261-b5fgb9wf	93	14	and	and	CC
cord-007261-b5fgb9wf	93	15	0.1	0.1	CD
cord-007261-b5fgb9wf	93	16	%	%	NN
cord-007261-b5fgb9wf	93	17	Tween-20	Tween-20	NNP
cord-007261-b5fgb9wf	93	18	,	,	,
cord-007261-b5fgb9wf	93	19	and	and	CC
cord-007261-b5fgb9wf	93	20	then	then	RB
cord-007261-b5fgb9wf	93	21	treated	treat	VBN
cord-007261-b5fgb9wf	93	22	with	with	IN
cord-007261-b5fgb9wf	93	23	appropriate	appropriate	JJ
cord-007261-b5fgb9wf	93	24	antibodies	antibody	NNS
cord-007261-b5fgb9wf	93	25	for	for	IN
cord-007261-b5fgb9wf	93	26	1	1	CD
cord-007261-b5fgb9wf	93	27	h.	h.	NN
cord-007261-b5fgb9wf	94	1	The	the	DT
cord-007261-b5fgb9wf	94	2	niters	niter	NNS
cord-007261-b5fgb9wf	94	3	were	be	VBD
cord-007261-b5fgb9wf	94	4	washed	wash	VBN
cord-007261-b5fgb9wf	94	5	with	with	IN
cord-007261-b5fgb9wf	94	6	PBS	PBS	NNP
cord-007261-b5fgb9wf	94	7	and	and	CC
cord-007261-b5fgb9wf	94	8	then	then	RB
cord-007261-b5fgb9wf	94	9	treated	treat	VBN
cord-007261-b5fgb9wf	94	10	with	with	IN
cord-007261-b5fgb9wf	94	11	horseradish	horseradish	NN
cord-007261-b5fgb9wf	94	12	peroxida8e	peroxida8e	NNP
cord-007261-b5fgb9wf	94	13	-	-	HYPH
cord-007261-b5fgb9wf	94	14	conjugated	conjugate	VBN
cord-007261-b5fgb9wf	94	15	goat	goat	NN
cord-007261-b5fgb9wf	94	16	anti	anti	JJ
cord-007261-b5fgb9wf	94	17	-	-	JJ
cord-007261-b5fgb9wf	94	18	rabbit	rabbit	JJ
cord-007261-b5fgb9wf	94	19	IgG	IgG	NNP
cord-007261-b5fgb9wf	94	20	antibodies	antibody	NNS
cord-007261-b5fgb9wf	94	21	in	in	IN
cord-007261-b5fgb9wf	94	22	PBS	PBS	NNP
cord-007261-b5fgb9wf	94	23	for	for	IN
cord-007261-b5fgb9wf	94	24	2	2	CD
cord-007261-b5fgb9wf	94	25	h.	h.	NN
cord-007261-b5fgb9wf	95	1	After	after	IN
cord-007261-b5fgb9wf	95	2	washing	washing	NN
cord-007261-b5fgb9wf	95	3	5	5	CD
cord-007261-b5fgb9wf	95	4	times	time	NNS
cord-007261-b5fgb9wf	95	5	for	for	IN
cord-007261-b5fgb9wf	95	6	5	5	CD
cord-007261-b5fgb9wf	95	7	min	min	NN
cord-007261-b5fgb9wf	95	8	each	each	DT
cord-007261-b5fgb9wf	95	9	,	,	,
cord-007261-b5fgb9wf	95	10	the	the	DT
cord-007261-b5fgb9wf	95	11	immunoreacted	immunoreacted	JJ
cord-007261-b5fgb9wf	95	12	bands	band	NNS
cord-007261-b5fgb9wf	95	13	were	be	VBD
cord-007261-b5fgb9wf	95	14	visualized	visualize	VBN
cord-007261-b5fgb9wf	95	15	with	with	IN
cord-007261-b5fgb9wf	95	16	an	an	DT
cord-007261-b5fgb9wf	95	17	ECL	ECL	NNP
cord-007261-b5fgb9wf	95	18	Western	western	JJ
cord-007261-b5fgb9wf	95	19	blotting	blot	VBG
cord-007261-b5fgb9wf	95	20	analysis	analysis	NN
cord-007261-b5fgb9wf	95	21	system	system	NN
cord-007261-b5fgb9wf	95	22	(	(	-LRB-
cord-007261-b5fgb9wf	95	23	Amersham	Amersham	NNP
cord-007261-b5fgb9wf	95	24	,	,	,
cord-007261-b5fgb9wf	95	25	U.K.	U.K.	NNP
cord-007261-b5fgb9wf	95	26	)	)	-RRB-
cord-007261-b5fgb9wf	95	27	.	.	.
cord-007261-b5fgb9wf	96	1	Enzyme	Enzyme	NNP
cord-007261-b5fgb9wf	96	2	Digestion	digestion	NN
cord-007261-b5fgb9wf	96	3	-	-	HYPH
cord-007261-b5fgb9wf	96	4	For	for	IN
cord-007261-b5fgb9wf	96	5	endoglycosidase	endoglycosidase	NN
cord-007261-b5fgb9wf	96	6	H	H	NNP
cord-007261-b5fgb9wf	96	7	and	and	CC
cord-007261-b5fgb9wf	96	8	iV	iV	NNP
cord-007261-b5fgb9wf	96	9	-	-	HYPH
cord-007261-b5fgb9wf	96	10	glycosidase	glycosidase	NNP
cord-007261-b5fgb9wf	96	11	F	F	NNP
cord-007261-b5fgb9wf	96	12	digestions	digestion	NNS
cord-007261-b5fgb9wf	96	13	,	,	,
cord-007261-b5fgb9wf	96	14	protein	protein	NN
cord-007261-b5fgb9wf	96	15	samples	sample	NNS
cord-007261-b5fgb9wf	96	16	were	be	VBD
cord-007261-b5fgb9wf	96	17	precipitated	precipitate	VBN
cord-007261-b5fgb9wf	96	18	with	with	IN
cord-007261-b5fgb9wf	96	19	9	9	CD
cord-007261-b5fgb9wf	96	20	volumes	volume	NNS
cord-007261-b5fgb9wf	96	21	of	of	IN
cord-007261-b5fgb9wf	96	22	ice	ice	NN
cord-007261-b5fgb9wf	96	23	-	-	HYPH
cord-007261-b5fgb9wf	96	24	cold	cold	JJ
cord-007261-b5fgb9wf	96	25	acetone	acetone	NN
cord-007261-b5fgb9wf	96	26	.	.	.
cord-007261-b5fgb9wf	97	1	After	after	IN
cord-007261-b5fgb9wf	97	2	centrifugation	centrifugation	NN
cord-007261-b5fgb9wf	97	3	,	,	,
cord-007261-b5fgb9wf	97	4	the	the	DT
cord-007261-b5fgb9wf	97	5	pellets	pellet	NNS
cord-007261-b5fgb9wf	97	6	were	be	VBD
cord-007261-b5fgb9wf	97	7	dissolved	dissolve	VBN
cord-007261-b5fgb9wf	97	8	in	in	IN
cord-007261-b5fgb9wf	97	9	50	50	CD
cord-007261-b5fgb9wf	97	10	mM	mm	CD
cord-007261-b5fgb9wf	97	11	sodium	sodium	NN
cord-007261-b5fgb9wf	97	12	citrate	citrate	NN
cord-007261-b5fgb9wf	97	13	buffer	buffer	NN
cord-007261-b5fgb9wf	97	14	(	(	-LRB-
cord-007261-b5fgb9wf	97	15	pH	pH	NNP
cord-007261-b5fgb9wf	97	16	5.0	5.0	CD
cord-007261-b5fgb9wf	97	17	)	)	-RRB-
cord-007261-b5fgb9wf	97	18	containing	contain	VBG
cord-007261-b5fgb9wf	97	19	2	2	CD
cord-007261-b5fgb9wf	97	20	%	%	NN
cord-007261-b5fgb9wf	97	21	SDS	SDS	NNP
cord-007261-b5fgb9wf	97	22	,	,	,
cord-007261-b5fgb9wf	97	23	and	and	CC
cord-007261-b5fgb9wf	97	24	then	then	RB
cord-007261-b5fgb9wf	97	25	the	the	DT
cord-007261-b5fgb9wf	97	26	solutions	solution	NNS
cord-007261-b5fgb9wf	97	27	were	be	VBD
cord-007261-b5fgb9wf	97	28	heated	heat	VBN
cord-007261-b5fgb9wf	97	29	at	at	IN
cord-007261-b5fgb9wf	97	30	100'C	100'c	CD
cord-007261-b5fgb9wf	97	31	for	for	IN
cord-007261-b5fgb9wf	97	32	3	3	CD
cord-007261-b5fgb9wf	97	33	min	min	NN
cord-007261-b5fgb9wf	97	34	.	.	.
cord-007261-b5fgb9wf	98	1	Nine	nine	CD
cord-007261-b5fgb9wf	98	2	volumes	volume	NNS
cord-007261-b5fgb9wf	98	3	of	of	IN
cord-007261-b5fgb9wf	98	4	50	50	CD
cord-007261-b5fgb9wf	98	5	mM	mm	CD
cord-007261-b5fgb9wf	98	6	sodium	sodium	NN
cord-007261-b5fgb9wf	98	7	citrate	citrate	NN
cord-007261-b5fgb9wf	98	8	buffer	buffer	NN
cord-007261-b5fgb9wf	98	9	(	(	-LRB-
cord-007261-b5fgb9wf	98	10	pH	pH	NNP
cord-007261-b5fgb9wf	98	11	5.0	5.0	CD
cord-007261-b5fgb9wf	98	12	)	)	-RRB-
cord-007261-b5fgb9wf	98	13	containing	contain	VBG
cord-007261-b5fgb9wf	98	14	2	2	CD
cord-007261-b5fgb9wf	98	15	%	%	NN
cord-007261-b5fgb9wf	99	1	Triton	Triton	NNP
cord-007261-b5fgb9wf	99	2	X-100	x-100	NN
cord-007261-b5fgb9wf	99	3	was	be	VBD
cord-007261-b5fgb9wf	99	4	then	then	RB
cord-007261-b5fgb9wf	99	5	added	add	VBN
cord-007261-b5fgb9wf	99	6	to	to	IN
cord-007261-b5fgb9wf	99	7	each	each	DT
cord-007261-b5fgb9wf	99	8	sample	sample	NN
cord-007261-b5fgb9wf	99	9	.	.	.
cord-007261-b5fgb9wf	100	1	Endo	Endo	NNP
cord-007261-b5fgb9wf	100	2	H	H	NNP
cord-007261-b5fgb9wf	100	3	(	(	-LRB-
cord-007261-b5fgb9wf	100	4	7.5mU	7.5mu	CD
cord-007261-b5fgb9wf	100	5	/	/	SYM
cord-007261-b5fgb9wf	100	6	ml	ml	NN
cord-007261-b5fgb9wf	100	7	)	)	-RRB-
cord-007261-b5fgb9wf	101	1	(	(	-LRB-
cord-007261-b5fgb9wf	101	2	Boehringer	Boehringer	NNP
cord-007261-b5fgb9wf	101	3	Mannheim	Mannheim	NNP
cord-007261-b5fgb9wf	101	4	GmbH	GmbH	NNP
cord-007261-b5fgb9wf	101	5	,	,	,
cord-007261-b5fgb9wf	101	6	Germany	Germany	NNP
cord-007261-b5fgb9wf	101	7	)	)	-RRB-
cord-007261-b5fgb9wf	101	8	was	be	VBD
cord-007261-b5fgb9wf	101	9	added	add	VBN
cord-007261-b5fgb9wf	101	10	to	to	IN
cord-007261-b5fgb9wf	101	11	the	the	DT
cord-007261-b5fgb9wf	101	12	samples	sample	NNS
cord-007261-b5fgb9wf	101	13	,	,	,
cord-007261-b5fgb9wf	101	14	which	which	WDT
cord-007261-b5fgb9wf	101	15	were	be	VBD
cord-007261-b5fgb9wf	101	16	then	then	RB
cord-007261-b5fgb9wf	101	17	incubated	incubate	VBN
cord-007261-b5fgb9wf	101	18	at	at	IN
cord-007261-b5fgb9wf	101	19	37'C	37'c	CD
cord-007261-b5fgb9wf	101	20	for	for	IN
cord-007261-b5fgb9wf	101	21	24	24	CD
cord-007261-b5fgb9wf	101	22	h.	h.	NN
cord-007261-b5fgb9wf	101	23	iV-	iV-	NNP
cord-007261-b5fgb9wf	102	1	Glycosidase	Glycosidase	NNP
cord-007261-b5fgb9wf	102	2	F	F	NNP
cord-007261-b5fgb9wf	102	3	(	(	-LRB-
cord-007261-b5fgb9wf	102	4	Boehringer	Boehringer	NNP
cord-007261-b5fgb9wf	102	5	Mannheim	Mannheim	NNP
cord-007261-b5fgb9wf	102	6	GmbH	GmbH	NNP
cord-007261-b5fgb9wf	102	7	)	)	-RRB-
cord-007261-b5fgb9wf	102	8	digestion	digestion	NN
cord-007261-b5fgb9wf	102	9	was	be	VBD
cord-007261-b5fgb9wf	102	10	performed	perform	VBN
cord-007261-b5fgb9wf	102	11	as	as	IN
cord-007261-b5fgb9wf	102	12	described	describe	VBN
cord-007261-b5fgb9wf	102	13	for	for	IN
cord-007261-b5fgb9wf	102	14	endo	endo	NN
cord-007261-b5fgb9wf	102	15	H	H	NNP
cord-007261-b5fgb9wf	102	16	digestion	digestion	NN
cord-007261-b5fgb9wf	102	17	except	except	IN
cord-007261-b5fgb9wf	102	18	for	for	IN
cord-007261-b5fgb9wf	102	19	the	the	DT
cord-007261-b5fgb9wf	102	20	following	following	JJ
cord-007261-b5fgb9wf	102	21	modifications	modification	NNS
cord-007261-b5fgb9wf	102	22	;	;	:
cord-007261-b5fgb9wf	102	23	sodium	sodium	NN
cord-007261-b5fgb9wf	102	24	phosphate	phosphate	NN
cord-007261-b5fgb9wf	102	25	buffer	buffer	NN
cord-007261-b5fgb9wf	102	26	(	(	-LRB-
cord-007261-b5fgb9wf	102	27	pH	pH	NNP
cord-007261-b5fgb9wf	102	28	7.5	7.5	CD
cord-007261-b5fgb9wf	102	29	)	)	-RRB-
cord-007261-b5fgb9wf	102	30	was	be	VBD
cord-007261-b5fgb9wf	102	31	used	use	VBN
cord-007261-b5fgb9wf	102	32	instead	instead	RB
cord-007261-b5fgb9wf	102	33	of	of	IN
cord-007261-b5fgb9wf	102	34	sodium	sodium	NN
cord-007261-b5fgb9wf	102	35	citrate	citrate	NN
cord-007261-b5fgb9wf	102	36	buffer	buffer	NN
cord-007261-b5fgb9wf	102	37	(	(	-LRB-
cord-007261-b5fgb9wf	102	38	pH	pH	NNP
cord-007261-b5fgb9wf	102	39	5.0	5.0	CD
cord-007261-b5fgb9wf	102	40	)	)	-RRB-
cord-007261-b5fgb9wf	102	41	,	,	,
cord-007261-b5fgb9wf	102	42	and	and	CC
cord-007261-b5fgb9wf	102	43	the	the	DT
cord-007261-b5fgb9wf	102	44	enzyme	enzyme	NN
cord-007261-b5fgb9wf	102	45	concentration	concentration	NN
cord-007261-b5fgb9wf	102	46	was	be	VBD
cord-007261-b5fgb9wf	102	47	20	20	CD
cord-007261-b5fgb9wf	102	48	mU	mU	NNS
cord-007261-b5fgb9wf	102	49	/	/	SYM
cord-007261-b5fgb9wf	102	50	ml	ml	NN
cord-007261-b5fgb9wf	102	51	.	.	.
cord-007261-b5fgb9wf	103	1	For	for	IN
cord-007261-b5fgb9wf	103	2	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	103	3	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	103	4	digestion	digestion	NN
cord-007261-b5fgb9wf	103	5	,	,	,
cord-007261-b5fgb9wf	103	6	the	the	DT
cord-007261-b5fgb9wf	103	7	protein	protein	NN
cord-007261-b5fgb9wf	103	8	samples	sample	NNS
cord-007261-b5fgb9wf	103	9	were	be	VBD
cord-007261-b5fgb9wf	103	10	heated	heat	VBN
cord-007261-b5fgb9wf	103	11	at	at	IN
cord-007261-b5fgb9wf	103	12	100'C	100'c	CD
cord-007261-b5fgb9wf	103	13	in	in	IN
cord-007261-b5fgb9wf	103	14	0.1	0.1	CD
cord-007261-b5fgb9wf	103	15	%	%	NN
cord-007261-b5fgb9wf	103	16	SDS	sds	NN
cord-007261-b5fgb9wf	103	17	for	for	IN
cord-007261-b5fgb9wf	103	18	3	3	CD
cord-007261-b5fgb9wf	103	19	min	min	NN
cord-007261-b5fgb9wf	103	20	and	and	CC
cord-007261-b5fgb9wf	103	21	then	then	RB
cord-007261-b5fgb9wf	103	22	incubated	incubate	VBN
cord-007261-b5fgb9wf	103	23	with	with	IN
cord-007261-b5fgb9wf	103	24	calf	calf	NN
cord-007261-b5fgb9wf	103	25	intestinal	intestinal	JJ
cord-007261-b5fgb9wf	103	26	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	103	27	(	(	-LRB-
cord-007261-b5fgb9wf	103	28	1	1	CD
cord-007261-b5fgb9wf	103	29	U	u	NN
cord-007261-b5fgb9wf	103	30	/	/	SYM
cord-007261-b5fgb9wf	103	31	ml	ml	NN
cord-007261-b5fgb9wf	103	32	)	)	-RRB-
cord-007261-b5fgb9wf	103	33	(	(	-LRB-
cord-007261-b5fgb9wf	103	34	Boehringer	Boehringer	NNP
cord-007261-b5fgb9wf	103	35	Mannheim	Mannheim	NNP
cord-007261-b5fgb9wf	103	36	GmbH	GmbH	NNP
cord-007261-b5fgb9wf	103	37	)	)	-RRB-
cord-007261-b5fgb9wf	103	38	at	at	IN
cord-007261-b5fgb9wf	103	39	30'C	30'c	CD
cord-007261-b5fgb9wf	103	40	for	for	IN
cord-007261-b5fgb9wf	103	41	1.5	1.5	CD
cord-007261-b5fgb9wf	103	42	h.	h.	NN
cord-007261-b5fgb9wf	103	43	Immunofluorescence	Immunofluorescence	NNP
cord-007261-b5fgb9wf	103	44	Microscopy	Microscopy	NNP
cord-007261-b5fgb9wf	103	45	-	-	HYPH
cord-007261-b5fgb9wf	103	46	Immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	103	47	microscopy	microscopy	NN
cord-007261-b5fgb9wf	103	48	was	be	VBD
cord-007261-b5fgb9wf	103	49	performed	perform	VBN
cord-007261-b5fgb9wf	103	50	by	by	IN
cord-007261-b5fgb9wf	103	51	the	the	DT
cord-007261-b5fgb9wf	103	52	method	method	NN
cord-007261-b5fgb9wf	103	53	described	describe	VBN
cord-007261-b5fgb9wf	103	54	by	by	IN
cord-007261-b5fgb9wf	103	55	Munro	Munro	NNP
cord-007261-b5fgb9wf	103	56	and	and	CC
cord-007261-b5fgb9wf	103	57	Pelham	Pelham	NNP
cord-007261-b5fgb9wf	103	58	(	(	-LRB-
cord-007261-b5fgb9wf	103	59	5	5	CD
cord-007261-b5fgb9wf	103	60	)	)	-RRB-
cord-007261-b5fgb9wf	103	61	.	.	.
cord-007261-b5fgb9wf	104	1	Five	five	CD
cord-007261-b5fgb9wf	104	2	micrograms	microgram	NNS
cord-007261-b5fgb9wf	104	3	each	each	DT
cord-007261-b5fgb9wf	104	4	of	of	IN
cord-007261-b5fgb9wf	104	5	the	the	DT
cord-007261-b5fgb9wf	104	6	plasmid	plasmid	NN
cord-007261-b5fgb9wf	105	1	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	105	2	harboring	harbor	VBG
cord-007261-b5fgb9wf	105	3	various	various	JJ
cord-007261-b5fgb9wf	105	4	cDNA	cdna	NN
cord-007261-b5fgb9wf	105	5	constructs	construct	NNS
cord-007261-b5fgb9wf	105	6	was	be	VBD
cord-007261-b5fgb9wf	105	7	transfected	transfecte	VBN
cord-007261-b5fgb9wf	105	8	into	into	IN
cord-007261-b5fgb9wf	105	9	COS	COS	NNP
cord-007261-b5fgb9wf	105	10	cells	cell	NNS
cord-007261-b5fgb9wf	105	11	in	in	IN
cord-007261-b5fgb9wf	105	12	a	a	DT
cord-007261-b5fgb9wf	105	13	3.5	3.5	CD
cord-007261-b5fgb9wf	105	14	cm	cm	NN
cord-007261-b5fgb9wf	105	15	dish	dish	NN
cord-007261-b5fgb9wf	105	16	.	.	.
cord-007261-b5fgb9wf	106	1	After	after	IN
cord-007261-b5fgb9wf	106	2	incubation	incubation	NN
cord-007261-b5fgb9wf	106	3	for	for	IN
cord-007261-b5fgb9wf	106	4	48	48	CD
cord-007261-b5fgb9wf	106	5	h	h	NN
cord-007261-b5fgb9wf	106	6	,	,	,
cord-007261-b5fgb9wf	106	7	the	the	DT
cord-007261-b5fgb9wf	106	8	cells	cell	NNS
cord-007261-b5fgb9wf	106	9	were	be	VBD
cord-007261-b5fgb9wf	106	10	fixed	fix	VBN
cord-007261-b5fgb9wf	106	11	with	with	IN
cord-007261-b5fgb9wf	106	12	2	2	CD
cord-007261-b5fgb9wf	106	13	%	%	NN
cord-007261-b5fgb9wf	106	14	paraformaldehyde	paraformaldehyde	NN
cord-007261-b5fgb9wf	106	15	and	and	CC
cord-007261-b5fgb9wf	106	16	0.1	0.1	CD
cord-007261-b5fgb9wf	106	17	%	%	NN
cord-007261-b5fgb9wf	106	18	glutaraldehyde	glutaraldehyde	VBN
cord-007261-b5fgb9wf	106	19	in	in	IN
cord-007261-b5fgb9wf	106	20	PBS	PBS	NNP
cord-007261-b5fgb9wf	106	21	for	for	IN
cord-007261-b5fgb9wf	106	22	10	10	CD
cord-007261-b5fgb9wf	106	23	min	min	NN
cord-007261-b5fgb9wf	106	24	,	,	,
cord-007261-b5fgb9wf	106	25	and	and	CC
cord-007261-b5fgb9wf	106	26	then	then	RB
cord-007261-b5fgb9wf	106	27	permeabilized	permeabilize	VBD
cord-007261-b5fgb9wf	106	28	with	with	IN
cord-007261-b5fgb9wf	106	29	1	1	CD
cord-007261-b5fgb9wf	106	30	%	%	NN
cord-007261-b5fgb9wf	106	31	Triton	Triton	NNP
cord-007261-b5fgb9wf	106	32	X-100	x-100	RB
cord-007261-b5fgb9wf	106	33	for	for	IN
cord-007261-b5fgb9wf	106	34	10	10	CD
cord-007261-b5fgb9wf	106	35	min	min	NN
cord-007261-b5fgb9wf	106	36	.	.	.
cord-007261-b5fgb9wf	107	1	The	the	DT
cord-007261-b5fgb9wf	107	2	fixed	fix	VBN
cord-007261-b5fgb9wf	107	3	cells	cell	NNS
cord-007261-b5fgb9wf	107	4	were	be	VBD
cord-007261-b5fgb9wf	107	5	treated	treat	VBN
cord-007261-b5fgb9wf	107	6	with	with	IN
cord-007261-b5fgb9wf	107	7	appropriate	appropriate	JJ
cord-007261-b5fgb9wf	107	8	antibodies	antibody	NNS
cord-007261-b5fgb9wf	107	9	,	,	,
cord-007261-b5fgb9wf	107	10	and	and	CC
cord-007261-b5fgb9wf	107	11	then	then	RB
cord-007261-b5fgb9wf	107	12	FITC	FITC	NNP
cord-007261-b5fgb9wf	107	13	-	-	HYPH
cord-007261-b5fgb9wf	107	14	conjugated	conjugate	VBN
cord-007261-b5fgb9wf	107	15	goat	goat	NN
cord-007261-b5fgb9wf	107	16	anti	anti	JJ
cord-007261-b5fgb9wf	107	17	-	-	JJ
cord-007261-b5fgb9wf	107	18	rabbit	rabbit	JJ
cord-007261-b5fgb9wf	107	19	IgG	igg	NN
cord-007261-b5fgb9wf	107	20	antibodies	antibody	NNS
cord-007261-b5fgb9wf	107	21	(	(	-LRB-
cord-007261-b5fgb9wf	107	22	Cappel	Cappel	NNP
cord-007261-b5fgb9wf	107	23	England	England	NNP
cord-007261-b5fgb9wf	107	24	)	)	-RRB-
cord-007261-b5fgb9wf	107	25	in	in	IN
cord-007261-b5fgb9wf	107	26	PBS	PBS	NNP
cord-007261-b5fgb9wf	107	27	containing	contain	VBG
cord-007261-b5fgb9wf	107	28	1	1	CD
cord-007261-b5fgb9wf	107	29	%	%	NN
cord-007261-b5fgb9wf	107	30	bovine	bovine	JJ
cord-007261-b5fgb9wf	107	31	serum	serum	NN
cord-007261-b5fgb9wf	107	32	albumin	albumin	NN
cord-007261-b5fgb9wf	107	33	.	.	.
cord-007261-b5fgb9wf	108	1	In	in	IN
cord-007261-b5fgb9wf	108	2	Vitro	Vitro	NNP
cord-007261-b5fgb9wf	108	3	Translation	Translation	NNP
cord-007261-b5fgb9wf	108	4	-	-	HYPH
cord-007261-b5fgb9wf	108	5	In	in	FW
cord-007261-b5fgb9wf	108	6	vitro	vitro	FW
cord-007261-b5fgb9wf	108	7	transcription	transcription	NN
cord-007261-b5fgb9wf	108	8	and	and	CC
cord-007261-b5fgb9wf	108	9	translation	translation	NN
cord-007261-b5fgb9wf	108	10	were	be	VBD
cord-007261-b5fgb9wf	108	11	performed	perform	VBN
cord-007261-b5fgb9wf	108	12	as	as	IN
cord-007261-b5fgb9wf	108	13	described	describe	VBN
cord-007261-b5fgb9wf	108	14	(	(	-LRB-
cord-007261-b5fgb9wf	108	15	11	11	CD
cord-007261-b5fgb9wf	108	16	)	)	-RRB-
cord-007261-b5fgb9wf	108	17	.	.	.
cord-007261-b5fgb9wf	109	1	Messenger	Messenger	NNP
cord-007261-b5fgb9wf	109	2	RNAs	rna	NNS
cord-007261-b5fgb9wf	109	3	transcribed	transcribe	VBN
cord-007261-b5fgb9wf	109	4	with	with	IN
cord-007261-b5fgb9wf	109	5	T7	t7	NN
cord-007261-b5fgb9wf	109	6	polymerase	polymerase	NN
cord-007261-b5fgb9wf	109	7	from	from	IN
cord-007261-b5fgb9wf	109	8	the	the	DT
cord-007261-b5fgb9wf	109	9	pSG5	pSG5	NNP
cord-007261-b5fgb9wf	109	10	plasmids	plasmid	NNS
cord-007261-b5fgb9wf	109	11	harboring	harbor	VBG
cord-007261-b5fgb9wf	109	12	various	various	JJ
cord-007261-b5fgb9wf	109	13	cDNA	cdna	NN
cord-007261-b5fgb9wf	109	14	constructs	construct	NNS
cord-007261-b5fgb9wf	109	15	were	be	VBD
cord-007261-b5fgb9wf	109	16	translated	translate	VBN
cord-007261-b5fgb9wf	109	17	in	in	IN
cord-007261-b5fgb9wf	109	18	a	a	DT
cord-007261-b5fgb9wf	109	19	wheat	wheat	NN
cord-007261-b5fgb9wf	109	20	germ	germ	NN
cord-007261-b5fgb9wf	109	21	cell	cell	NN
cord-007261-b5fgb9wf	109	22	-	-	HYPH
cord-007261-b5fgb9wf	109	23	free	free	JJ
cord-007261-b5fgb9wf	109	24	system	system	NN
cord-007261-b5fgb9wf	109	25	in	in	IN
cord-007261-b5fgb9wf	109	26	the	the	DT
cord-007261-b5fgb9wf	109	27	presence	presence	NN
cord-007261-b5fgb9wf	109	28	of	of	IN
cord-007261-b5fgb9wf	109	29	dog	dog	NN
cord-007261-b5fgb9wf	109	30	pancreas	pancrea	VBZ
cord-007261-b5fgb9wf	109	31	rough	rough	JJ
cord-007261-b5fgb9wf	109	32	micro8omes	micro8ome	NNS
cord-007261-b5fgb9wf	109	33	for	for	IN
cord-007261-b5fgb9wf	109	34	30	30	CD
cord-007261-b5fgb9wf	109	35	min	min	NN
cord-007261-b5fgb9wf	109	36	at	at	IN
cord-007261-b5fgb9wf	109	37	26'C.	26'c.	CD
cord-007261-b5fgb9wf	109	38	Analytical	Analytical	NNP
cord-007261-b5fgb9wf	109	39	Methods	Methods	NNPS
cord-007261-b5fgb9wf	109	40	-	-	HYPH
cord-007261-b5fgb9wf	109	41	Esterase	Esterase	NNP
cord-007261-b5fgb9wf	109	42	activity	activity	NN
cord-007261-b5fgb9wf	109	43	was	be	VBD
cord-007261-b5fgb9wf	109	44	measured	measure	VBN
cord-007261-b5fgb9wf	109	45	spectrophotometrically	spectrophotometrically	RB
cord-007261-b5fgb9wf	109	46	using	use	VBG
cord-007261-b5fgb9wf	109	47	p	p	NN
cord-007261-b5fgb9wf	109	48	-	-	HYPH
cord-007261-b5fgb9wf	109	49	nitrophenyl	nitrophenyl	NN
cord-007261-b5fgb9wf	109	50	acetate	acetate	NN
cord-007261-b5fgb9wf	109	51	in	in	IN
cord-007261-b5fgb9wf	109	52	100	100	CD
cord-007261-b5fgb9wf	109	53	mM	mm	CD
cord-007261-b5fgb9wf	109	54	Tris	Tris	NNP
cord-007261-b5fgb9wf	109	55	-	-	HYPH
cord-007261-b5fgb9wf	109	56	HCl	HCl	NNP
cord-007261-b5fgb9wf	109	57	buffer	buffer	NN
cord-007261-b5fgb9wf	109	58	(	(	-LRB-
cord-007261-b5fgb9wf	109	59	pH8.2	pH8.2	NNP
cord-007261-b5fgb9wf	109	60	)	)	-RRB-
cord-007261-b5fgb9wf	109	61	at	at	IN
cord-007261-b5fgb9wf	109	62	405	405	CD
cord-007261-b5fgb9wf	109	63	nm	nm	NN
cord-007261-b5fgb9wf	109	64	according	accord	VBG
cord-007261-b5fgb9wf	109	65	to	to	IN
cord-007261-b5fgb9wf	109	66	Krisch	Krisch	NNP
cord-007261-b5fgb9wf	109	67	(	(	-LRB-
cord-007261-b5fgb9wf	109	68	22	22	CD
cord-007261-b5fgb9wf	109	69	)	)	-RRB-
cord-007261-b5fgb9wf	109	70	.	.	.
cord-007261-b5fgb9wf	110	1	NADPH	nadph	NN
cord-007261-b5fgb9wf	110	2	-	-	HYPH
cord-007261-b5fgb9wf	110	3	cytochrome	cytochrome	NNP
cord-007261-b5fgb9wf	111	1	c	c	NNP
cord-007261-b5fgb9wf	111	2	reductase	reductase	NNP
cord-007261-b5fgb9wf	111	3	activity	activity	NN
cord-007261-b5fgb9wf	111	4	was	be	VBD
cord-007261-b5fgb9wf	111	5	measured	measure	VBN
cord-007261-b5fgb9wf	111	6	as	as	IN
cord-007261-b5fgb9wf	111	7	described	describe	VBN
cord-007261-b5fgb9wf	111	8	(	(	-LRB-
cord-007261-b5fgb9wf	111	9	23	23	CD
cord-007261-b5fgb9wf	111	10	)	)	-RRB-
cord-007261-b5fgb9wf	111	11	.	.	.
cord-007261-b5fgb9wf	112	1	a	a	LS
cord-007261-b5fgb9wf	112	2	-	-	:
cord-007261-b5fgb9wf	112	3	Mannosidase	Mannosidase	NNP
cord-007261-b5fgb9wf	112	4	II	II	NNP
cord-007261-b5fgb9wf	112	5	activity	activity	NN
cord-007261-b5fgb9wf	112	6	was	be	VBD
cord-007261-b5fgb9wf	112	7	measured	measure	VBN
cord-007261-b5fgb9wf	112	8	as	as	IN
cord-007261-b5fgb9wf	112	9	described	describe	VBN
cord-007261-b5fgb9wf	112	10	(	(	-LRB-
cord-007261-b5fgb9wf	112	11	24	24	CD
cord-007261-b5fgb9wf	112	12	)	)	-RRB-
cord-007261-b5fgb9wf	112	13	.	.	.
cord-007261-b5fgb9wf	113	1	for	for	IN
cord-007261-b5fgb9wf	113	2	the	the	DT
cord-007261-b5fgb9wf	113	3	Retention	Retention	NNP
cord-007261-b5fgb9wf	113	4	to	to	IN
cord-007261-b5fgb9wf	113	5	ER	ER	NNP
cord-007261-b5fgb9wf	113	6	-	-	:
cord-007261-b5fgb9wf	113	7	To	to	TO
cord-007261-b5fgb9wf	113	8	identify	identify	VB
cord-007261-b5fgb9wf	113	9	the	the	DT
cord-007261-b5fgb9wf	113	10	regions	region	NNS
cord-007261-b5fgb9wf	113	11	that	that	WDT
cord-007261-b5fgb9wf	113	12	are	be	VBP
cord-007261-b5fgb9wf	113	13	responsible	responsible	JJ
cord-007261-b5fgb9wf	113	14	for	for	IN
cord-007261-b5fgb9wf	113	15	the	the	DT
cord-007261-b5fgb9wf	113	16	retention	retention	NN
cord-007261-b5fgb9wf	113	17	of	of	IN
cord-007261-b5fgb9wf	113	18	P450(Ml	P450(Ml	NNP
cord-007261-b5fgb9wf	113	19	)	)	-RRB-
cord-007261-b5fgb9wf	113	20	in	in	IN
cord-007261-b5fgb9wf	113	21	the	the	DT
cord-007261-b5fgb9wf	113	22	ER	ER	NNP
cord-007261-b5fgb9wf	113	23	,	,	,
cord-007261-b5fgb9wf	113	24	we	-PRON-	PRP
cord-007261-b5fgb9wf	113	25	used	use	VBD
cord-007261-b5fgb9wf	113	26	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	113	27	Sec	Sec	NNP
cord-007261-b5fgb9wf	113	28	as	as	IN
cord-007261-b5fgb9wf	113	29	a	a	DT
cord-007261-b5fgb9wf	113	30	reporter	reporter	NN
cord-007261-b5fgb9wf	113	31	protein	protein	NN
cord-007261-b5fgb9wf	113	32	.	.	.
cord-007261-b5fgb9wf	114	1	Carboxyesterase	Carboxyesterase	NNP
cord-007261-b5fgb9wf	114	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	114	3	,	,	,
cord-007261-b5fgb9wf	114	4	an	an	DT
cord-007261-b5fgb9wf	114	5	isozyme	isozyme	NNS
cord-007261-b5fgb9wf	114	6	of	of	IN
cord-007261-b5fgb9wf	114	7	liver	liver	NN
cord-007261-b5fgb9wf	114	8	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	114	9	carboxyesterases	carboxyesterases	NNP
cord-007261-b5fgb9wf	114	10	,	,	,
cord-007261-b5fgb9wf	114	11	contains	contain	VBZ
cord-007261-b5fgb9wf	114	12	no	no	DT
cord-007261-b5fgb9wf	114	13	KDEL	KDEL	NNP
cord-007261-b5fgb9wf	114	14	-	-	HYPH
cord-007261-b5fgb9wf	114	15	type	type	NN
cord-007261-b5fgb9wf	114	16	sequence	sequence	NN
cord-007261-b5fgb9wf	114	17	at	at	IN
cord-007261-b5fgb9wf	114	18	the	the	DT
cord-007261-b5fgb9wf	114	19	C	C	NNP
cord-007261-b5fgb9wf	114	20	-	-	HYPH
cord-007261-b5fgb9wf	114	21	terminus	terminus	NN
cord-007261-b5fgb9wf	114	22	and	and	CC
cord-007261-b5fgb9wf	114	23	is	be	VBZ
cord-007261-b5fgb9wf	114	24	secreted	secrete	VBN
cord-007261-b5fgb9wf	114	25	into	into	IN
cord-007261-b5fgb9wf	114	26	the	the	DT
cord-007261-b5fgb9wf	114	27	blood	blood	NN
cord-007261-b5fgb9wf	114	28	as	as	IN
cord-007261-b5fgb9wf	114	29	a	a	DT
cord-007261-b5fgb9wf	114	30	glycoprotein	glycoprotein	NN
cord-007261-b5fgb9wf	114	31	with	with	IN
cord-007261-b5fgb9wf	114	32	complex	complex	JJ
cord-007261-b5fgb9wf	114	33	type	type	NN
cord-007261-b5fgb9wf	114	34	sugar	sugar	NN
cord-007261-b5fgb9wf	114	35	chains	chain	NNS
cord-007261-b5fgb9wf	114	36	.	.	.
cord-007261-b5fgb9wf	115	1	Thus	thus	RB
cord-007261-b5fgb9wf	115	2	,	,	,
cord-007261-b5fgb9wf	115	3	the	the	DT
cord-007261-b5fgb9wf	115	4	type	type	NN
cord-007261-b5fgb9wf	115	5	of	of	IN
cord-007261-b5fgb9wf	115	6	the	the	DT
cord-007261-b5fgb9wf	115	7	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	115	8	modification	modification	NN
cord-007261-b5fgb9wf	115	9	occurring	occur	VBG
cord-007261-b5fgb9wf	115	10	during	during	IN
cord-007261-b5fgb9wf	115	11	the	the	DT
cord-007261-b5fgb9wf	115	12	transit	transit	NN
cord-007261-b5fgb9wf	115	13	through	through	IN
cord-007261-b5fgb9wf	115	14	the	the	DT
cord-007261-b5fgb9wf	115	15	secretory	secretory	JJ
cord-007261-b5fgb9wf	115	16	pathway	pathway	NN
cord-007261-b5fgb9wf	115	17	is	be	VBZ
cord-007261-b5fgb9wf	115	18	a	a	DT
cord-007261-b5fgb9wf	115	19	good	good	JJ
cord-007261-b5fgb9wf	115	20	diagnostic	diagnostic	JJ
cord-007261-b5fgb9wf	115	21	clue	clue	NN
cord-007261-b5fgb9wf	115	22	as	as	IN
cord-007261-b5fgb9wf	115	23	to	to	IN
cord-007261-b5fgb9wf	115	24	the	the	DT
cord-007261-b5fgb9wf	115	25	localization	localization	NN
cord-007261-b5fgb9wf	115	26	of	of	IN
cord-007261-b5fgb9wf	115	27	the	the	DT
cord-007261-b5fgb9wf	115	28	enzyme	enzyme	NN
cord-007261-b5fgb9wf	115	29	.	.	.
cord-007261-b5fgb9wf	116	1	Precarboxyesterase	Precarboxyesterase	NNP
cord-007261-b5fgb9wf	116	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	116	3	was	be	VBD
cord-007261-b5fgb9wf	116	4	fused	fuse	VBN
cord-007261-b5fgb9wf	116	5	at	at	IN
cord-007261-b5fgb9wf	116	6	the	the	DT
cord-007261-b5fgb9wf	116	7	cDNA	cdna	NN
cord-007261-b5fgb9wf	116	8	level	level	NN
cord-007261-b5fgb9wf	116	9	to	to	IN
cord-007261-b5fgb9wf	116	10	the	the	DT
cord-007261-b5fgb9wf	116	11	amino	amino	NN
cord-007261-b5fgb9wf	116	12	terminus	terminus	NN
cord-007261-b5fgb9wf	116	13	of	of	IN
cord-007261-b5fgb9wf	116	14	intact	intact	JJ
cord-007261-b5fgb9wf	116	15	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	116	16	)	)	-RRB-
cord-007261-b5fgb9wf	116	17	or	or	CC
cord-007261-b5fgb9wf	116	18	modified	modify	VBN
cord-007261-b5fgb9wf	116	19	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	116	20	)	)	-RRB-
cord-007261-b5fgb9wf	116	21	,	,	,
cord-007261-b5fgb9wf	116	22	with	with	IN
cord-007261-b5fgb9wf	116	23	various	various	JJ
cord-007261-b5fgb9wf	116	24	deletions	deletion	NNS
cord-007261-b5fgb9wf	116	25	from	from	IN
cord-007261-b5fgb9wf	116	26	the	the	DT
cord-007261-b5fgb9wf	116	27	C	C	NNP
cord-007261-b5fgb9wf	116	28	-	-	HYPH
cord-007261-b5fgb9wf	116	29	terminal	terminal	NN
cord-007261-b5fgb9wf	116	30	region	region	NN
cord-007261-b5fgb9wf	116	31	,	,	,
cord-007261-b5fgb9wf	116	32	into	into	IN
cord-007261-b5fgb9wf	116	33	the	the	DT
cord-007261-b5fgb9wf	116	34	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	116	35	segment	segment	NN
cord-007261-b5fgb9wf	116	36	(	(	-LRB-
cord-007261-b5fgb9wf	116	37	Fig	Fig	NNP
cord-007261-b5fgb9wf	116	38	.	.	.
cord-007261-b5fgb9wf	116	39	1	1	CD
cord-007261-b5fgb9wf	116	40	)	)	-RRB-
cord-007261-b5fgb9wf	116	41	.	.	.
cord-007261-b5fgb9wf	117	1	The	the	DT
cord-007261-b5fgb9wf	117	2	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	117	3	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	117	4	were	be	VBD
cord-007261-b5fgb9wf	117	5	transfected	transfecte	VBN
cord-007261-b5fgb9wf	117	6	into	into	IN
cord-007261-b5fgb9wf	117	7	COS	COS	NNP
cord-007261-b5fgb9wf	117	8	cells	cell	NNS
cord-007261-b5fgb9wf	117	9	.	.	.
cord-007261-b5fgb9wf	118	1	Forty	forty	CD
cord-007261-b5fgb9wf	118	2	hours	hour	NNS
cord-007261-b5fgb9wf	118	3	later	later	RB
cord-007261-b5fgb9wf	118	4	,	,	,
cord-007261-b5fgb9wf	118	5	the	the	DT
cord-007261-b5fgb9wf	118	6	cells	cell	NNS
cord-007261-b5fgb9wf	118	7	were	be	VBD
cord-007261-b5fgb9wf	118	8	labeled	label	VBN
cord-007261-b5fgb9wf	118	9	with	with	IN
cord-007261-b5fgb9wf	118	10	[	[	-LRB-
cord-007261-b5fgb9wf	118	11	35	35	CD
cord-007261-b5fgb9wf	118	12	S]meth	S]meth	NNP
cord-007261-b5fgb9wf	118	13	-	-	HYPH
cord-007261-b5fgb9wf	118	14	ionine	ionine	NN
cord-007261-b5fgb9wf	118	15	for	for	IN
cord-007261-b5fgb9wf	118	16	3	3	CD
cord-007261-b5fgb9wf	118	17	h	h	NN
cord-007261-b5fgb9wf	118	18	and	and	CC
cord-007261-b5fgb9wf	118	19	then	then	RB
cord-007261-b5fgb9wf	118	20	incubated	incubate	VBN
cord-007261-b5fgb9wf	118	21	in	in	IN
cord-007261-b5fgb9wf	118	22	the	the	DT
cord-007261-b5fgb9wf	118	23	chase	chase	NN
cord-007261-b5fgb9wf	118	24	medium	medium	NN
cord-007261-b5fgb9wf	118	25	containing	contain	VBG
cord-007261-b5fgb9wf	118	26	non	non	JJ
cord-007261-b5fgb9wf	118	27	-	-	JJ
cord-007261-b5fgb9wf	118	28	radioactive	radioactive	JJ
cord-007261-b5fgb9wf	118	29	methionine	methionine	NN
cord-007261-b5fgb9wf	118	30	for	for	IN
cord-007261-b5fgb9wf	118	31	a	a	DT
cord-007261-b5fgb9wf	118	32	further	further	JJ
cord-007261-b5fgb9wf	118	33	3	3	CD
cord-007261-b5fgb9wf	118	34	h.	h.	NN
cord-007261-b5fgb9wf	119	1	The	the	DT
cord-007261-b5fgb9wf	119	2	labeled	label	VBN
cord-007261-b5fgb9wf	119	3	proteins	protein	NNS
cord-007261-b5fgb9wf	119	4	in	in	IN
cord-007261-b5fgb9wf	119	5	the	the	DT
cord-007261-b5fgb9wf	119	6	cells	cell	NNS
cord-007261-b5fgb9wf	119	7	and	and	CC
cord-007261-b5fgb9wf	119	8	medium	medium	NN
cord-007261-b5fgb9wf	119	9	were	be	VBD
cord-007261-b5fgb9wf	119	10	immunoprecipitated	immunoprecipitate	VBN
cord-007261-b5fgb9wf	119	11	with	with	IN
cord-007261-b5fgb9wf	119	12	anti	anti	JJ
cord-007261-b5fgb9wf	119	13	-	-	JJ
cord-007261-b5fgb9wf	119	14	Est	est	JJ
cord-007261-b5fgb9wf	119	15	antibodies	antibody	NNS
cord-007261-b5fgb9wf	119	16	,	,	,
cord-007261-b5fgb9wf	119	17	which	which	WDT
cord-007261-b5fgb9wf	119	18	cross	cross	NN
cord-007261-b5fgb9wf	119	19	-	-	VBD
cord-007261-b5fgb9wf	119	20	reacted	react	VBN
cord-007261-b5fgb9wf	119	21	with	with	IN
cord-007261-b5fgb9wf	119	22	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	119	23	Sec	Sec	NNP
cord-007261-b5fgb9wf	119	24	,	,	,
cord-007261-b5fgb9wf	119	25	and	and	CC
cord-007261-b5fgb9wf	119	26	then	then	RB
cord-007261-b5fgb9wf	119	27	analyzed	analyze	VBN
cord-007261-b5fgb9wf	119	28	by	by	IN
cord-007261-b5fgb9wf	119	29	SDS	SDS	NNP
cord-007261-b5fgb9wf	119	30	-	-	HYPH
cord-007261-b5fgb9wf	119	31	PAGE	PAGE	NNP
cord-007261-b5fgb9wf	119	32	.	.	.
cord-007261-b5fgb9wf	120	1	When	when	WRB
cord-007261-b5fgb9wf	120	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	120	3	DNA	DNA	NNP
cord-007261-b5fgb9wf	120	4	was	be	VBD
cord-007261-b5fgb9wf	120	5	transfected	transfecte	VBN
cord-007261-b5fgb9wf	120	6	into	into	IN
cord-007261-b5fgb9wf	120	7	COS	COS	NNP
cord-007261-b5fgb9wf	120	8	cells	cell	NNS
cord-007261-b5fgb9wf	120	9	,	,	,
cord-007261-b5fgb9wf	120	10	a	a	DT
cord-007261-b5fgb9wf	120	11	single	single	JJ
cord-007261-b5fgb9wf	120	12	immunoprecipitable	immunoprecipitable	JJ
cord-007261-b5fgb9wf	120	13	product	product	NN
cord-007261-b5fgb9wf	120	14	was	be	VBD
cord-007261-b5fgb9wf	120	15	detected	detect	VBN
cord-007261-b5fgb9wf	120	16	in	in	IN
cord-007261-b5fgb9wf	120	17	the	the	DT
cord-007261-b5fgb9wf	120	18	cells	cell	NNS
cord-007261-b5fgb9wf	120	19	after	after	IN
cord-007261-b5fgb9wf	120	20	3	3	CD
cord-007261-b5fgb9wf	120	21	h	h	NN
cord-007261-b5fgb9wf	120	22	pulse	pulse	NN
cord-007261-b5fgb9wf	120	23	-	-	HYPH
cord-007261-b5fgb9wf	120	24	labeling	labeling	NN
cord-007261-b5fgb9wf	120	25	(	(	-LRB-
cord-007261-b5fgb9wf	120	26	Fig	Fig	NNP
cord-007261-b5fgb9wf	120	27	.	.	.
cord-007261-b5fgb9wf	120	28	2A	2a	CD
cord-007261-b5fgb9wf	120	29	,	,	,
cord-007261-b5fgb9wf	120	30	lane	lane	NN
cord-007261-b5fgb9wf	120	31	1	1	CD
cord-007261-b5fgb9wf	120	32	)	)	-RRB-
cord-007261-b5fgb9wf	120	33	,	,	,
cord-007261-b5fgb9wf	120	34	and	and	CC
cord-007261-b5fgb9wf	120	35	a	a	DT
cord-007261-b5fgb9wf	120	36	significant	significant	JJ
cord-007261-b5fgb9wf	120	37	amount	amount	NN
cord-007261-b5fgb9wf	120	38	of	of	IN
cord-007261-b5fgb9wf	120	39	another	another	DT
cord-007261-b5fgb9wf	120	40	Sec	Sec	NNP
cord-007261-b5fgb9wf	120	41	product	product	NN
cord-007261-b5fgb9wf	120	42	with	with	IN
cord-007261-b5fgb9wf	120	43	lower	low	JJR
cord-007261-b5fgb9wf	120	44	mobility	mobility	NN
cord-007261-b5fgb9wf	120	45	appeared	appear	VBD
cord-007261-b5fgb9wf	120	46	in	in	IN
cord-007261-b5fgb9wf	120	47	the	the	DT
cord-007261-b5fgb9wf	120	48	medium	medium	NN
cord-007261-b5fgb9wf	120	49	(	(	-LRB-
cord-007261-b5fgb9wf	120	50	Fig	Fig	NNP
cord-007261-b5fgb9wf	120	51	.	.	.
cord-007261-b5fgb9wf	120	52	2A	2a	CD
cord-007261-b5fgb9wf	120	53	,	,	,
cord-007261-b5fgb9wf	120	54	lane	lane	NN
cord-007261-b5fgb9wf	120	55	7	7	CD
cord-007261-b5fgb9wf	120	56	)	)	-RRB-
cord-007261-b5fgb9wf	120	57	.	.	.
cord-007261-b5fgb9wf	121	1	After	after	IN
cord-007261-b5fgb9wf	121	2	a	a	DT
cord-007261-b5fgb9wf	121	3	3	3	CD
cord-007261-b5fgb9wf	121	4	h	h	NN
cord-007261-b5fgb9wf	121	5	chase	chase	NN
cord-007261-b5fgb9wf	121	6	,	,	,
cord-007261-b5fgb9wf	121	7	the	the	DT
cord-007261-b5fgb9wf	121	8	amount	amount	NN
cord-007261-b5fgb9wf	121	9	of	of	IN
cord-007261-b5fgb9wf	121	10	the	the	DT
cord-007261-b5fgb9wf	121	11	Sec	Sec	NNP
cord-007261-b5fgb9wf	121	12	product	product	NN
cord-007261-b5fgb9wf	121	13	in	in	IN
cord-007261-b5fgb9wf	121	14	the	the	DT
cord-007261-b5fgb9wf	121	15	cells	cell	NNS
cord-007261-b5fgb9wf	121	16	decreased	decrease	VBD
cord-007261-b5fgb9wf	121	17	and	and	CC
cord-007261-b5fgb9wf	121	18	that	that	IN
cord-007261-b5fgb9wf	121	19	in	in	IN
cord-007261-b5fgb9wf	121	20	the	the	DT
cord-007261-b5fgb9wf	121	21	medium	medium	NN
cord-007261-b5fgb9wf	121	22	increased	increase	VBD
cord-007261-b5fgb9wf	121	23	significantly	significantly	RB
cord-007261-b5fgb9wf	121	24	(	(	-LRB-
cord-007261-b5fgb9wf	121	25	Fig	Fig	NNP
cord-007261-b5fgb9wf	121	26	.	.	.
cord-007261-b5fgb9wf	121	27	2A	2a	CD
cord-007261-b5fgb9wf	121	28	,	,	,
cord-007261-b5fgb9wf	121	29	lanes	lane	NNS
cord-007261-b5fgb9wf	121	30	4	4	CD
cord-007261-b5fgb9wf	121	31	and	and	CC
cord-007261-b5fgb9wf	121	32	 	 	_SP
cord-007261-b5fgb9wf	121	33	10	10	CD
cord-007261-b5fgb9wf	121	34	)	)	-RRB-
cord-007261-b5fgb9wf	121	35	.	.	.
cord-007261-b5fgb9wf	122	1	The	the	DT
cord-007261-b5fgb9wf	122	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	122	3	product	product	NN
cord-007261-b5fgb9wf	122	4	in	in	IN
cord-007261-b5fgb9wf	122	5	the	the	DT
cord-007261-b5fgb9wf	122	6	cells	cell	NNS
cord-007261-b5fgb9wf	122	7	was	be	VBD
cord-007261-b5fgb9wf	122	8	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	122	9	to	to	IN
cord-007261-b5fgb9wf	122	10	endoglycosidase	endoglycosidase	NNP
cord-007261-b5fgb9wf	122	11	H	h	NN
cord-007261-b5fgb9wf	122	12	treatment	treatment	NN
cord-007261-b5fgb9wf	123	1	(	(	-LRB-
cord-007261-b5fgb9wf	123	2	Fig	Fig	NNP
cord-007261-b5fgb9wf	123	3	.	.	.
cord-007261-b5fgb9wf	123	4	2A	2a	CD
cord-007261-b5fgb9wf	123	5	,	,	,
cord-007261-b5fgb9wf	123	6	lanes	lane	NNS
cord-007261-b5fgb9wf	123	7	2	2	CD
cord-007261-b5fgb9wf	123	8	and	and	CC
cord-007261-b5fgb9wf	123	9	5	5	CD
cord-007261-b5fgb9wf	123	10	)	)	-RRB-
cord-007261-b5fgb9wf	123	11	and	and	CC
cord-007261-b5fgb9wf	123	12	migrated	migrate	VBD
cord-007261-b5fgb9wf	123	13	with	with	IN
cord-007261-b5fgb9wf	123	14	slightly	slightly	RB
cord-007261-b5fgb9wf	123	15	lower	low	JJR
cord-007261-b5fgb9wf	123	16	mobility	mobility	NN
cord-007261-b5fgb9wf	123	17	in	in	IN
cord-007261-b5fgb9wf	123	18	the	the	DT
cord-007261-b5fgb9wf	123	19	gel	gel	NN
cord-007261-b5fgb9wf	123	20	than	than	IN
cord-007261-b5fgb9wf	123	21	the	the	DT
cord-007261-b5fgb9wf	123	22	unglycosylated	unglycosylated	JJ
cord-007261-b5fgb9wf	123	23	Sec	Sec	NNP
cord-007261-b5fgb9wf	123	24	product	product	NN
cord-007261-b5fgb9wf	123	25	synthesized	synthesize	VBN
cord-007261-b5fgb9wf	123	26	in	in	IN
cord-007261-b5fgb9wf	123	27	the	the	DT
cord-007261-b5fgb9wf	123	28	presence	presence	NN
cord-007261-b5fgb9wf	123	29	of	of	IN
cord-007261-b5fgb9wf	123	30	tunicamycin	tunicamycin	NNS
cord-007261-b5fgb9wf	123	31	(	(	-LRB-
cord-007261-b5fgb9wf	123	32	Fig	Fig	NNP
cord-007261-b5fgb9wf	123	33	.	.	.
cord-007261-b5fgb9wf	123	34	2A	2a	CD
cord-007261-b5fgb9wf	123	35	,	,	,
cord-007261-b5fgb9wf	123	36	lanes	lane	VBZ
cord-007261-b5fgb9wf	123	37	3	3	CD
cord-007261-b5fgb9wf	123	38	,	,	,
cord-007261-b5fgb9wf	123	39	6	6	CD
cord-007261-b5fgb9wf	123	40	,	,	,
cord-007261-b5fgb9wf	123	41	9	9	CD
cord-007261-b5fgb9wf	123	42	,	,	,
cord-007261-b5fgb9wf	123	43	and	and	CC
cord-007261-b5fgb9wf	123	44	12	12	CD
cord-007261-b5fgb9wf	123	45	)	)	-RRB-
cord-007261-b5fgb9wf	123	46	.	.	.
cord-007261-b5fgb9wf	124	1	The	the	DT
cord-007261-b5fgb9wf	124	2	slight	slight	JJ
cord-007261-b5fgb9wf	124	3	difference	difference	NN
cord-007261-b5fgb9wf	124	4	in	in	IN
cord-007261-b5fgb9wf	124	5	the	the	DT
cord-007261-b5fgb9wf	124	6	mobility	mobility	NN
cord-007261-b5fgb9wf	124	7	between	between	IN
cord-007261-b5fgb9wf	124	8	these	these	DT
cord-007261-b5fgb9wf	124	9	products	product	NNS
cord-007261-b5fgb9wf	124	10	was	be	VBD
cord-007261-b5fgb9wf	124	11	probably	probably	RB
cord-007261-b5fgb9wf	124	12	due	due	JJ
cord-007261-b5fgb9wf	124	13	to	to	IN
cord-007261-b5fgb9wf	124	14	the	the	DT
cord-007261-b5fgb9wf	124	15	fact	fact	NN
cord-007261-b5fgb9wf	124	16	that	that	IN
cord-007261-b5fgb9wf	124	17	endo	endo	NN
cord-007261-b5fgb9wf	124	18	H	H	NNP
cord-007261-b5fgb9wf	124	19	cleaved	cleave	VBD
cord-007261-b5fgb9wf	124	20	the	the	DT
cord-007261-b5fgb9wf	124	21	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	124	22	-	-	HYPH
cord-007261-b5fgb9wf	124	23	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	124	24	bond	bond	NN
cord-007261-b5fgb9wf	124	25	of	of	IN
cord-007261-b5fgb9wf	124	26	the	the	DT
cord-007261-b5fgb9wf	124	27	high	high	JJ
cord-007261-b5fgb9wf	124	28	mannose	mannose	NN
cord-007261-b5fgb9wf	124	29	type	type	NN
cord-007261-b5fgb9wf	124	30	sugar	sugar	NN
cord-007261-b5fgb9wf	124	31	chains	chain	NNS
cord-007261-b5fgb9wf	124	32	leaving	leave	VBG
cord-007261-b5fgb9wf	124	33	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	124	34	-	-	HYPH
cord-007261-b5fgb9wf	124	35	Asn	Asn	NNP
cord-007261-b5fgb9wf	124	36	in	in	IN
cord-007261-b5fgb9wf	124	37	the	the	DT
cord-007261-b5fgb9wf	124	38	substrate	substrate	NN
cord-007261-b5fgb9wf	124	39	molecule	molecule	NN
cord-007261-b5fgb9wf	124	40	.	.	.
cord-007261-b5fgb9wf	125	1	On	on	IN
cord-007261-b5fgb9wf	125	2	the	the	DT
cord-007261-b5fgb9wf	125	3	other	other	JJ
cord-007261-b5fgb9wf	125	4	hand	hand	NN
cord-007261-b5fgb9wf	125	5	,	,	,
cord-007261-b5fgb9wf	125	6	the	the	DT
cord-007261-b5fgb9wf	125	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	125	8	product	product	NN
cord-007261-b5fgb9wf	125	9	in	in	IN
cord-007261-b5fgb9wf	125	10	the	the	DT
cord-007261-b5fgb9wf	125	11	medium	medium	NN
cord-007261-b5fgb9wf	125	12	was	be	VBD
cord-007261-b5fgb9wf	125	13	more	more	RBR
cord-007261-b5fgb9wf	125	14	resistant	resistant	JJ
cord-007261-b5fgb9wf	125	15	to	to	IN
cord-007261-b5fgb9wf	125	16	endo	endo	FW
cord-007261-b5fgb9wf	125	17	H	h	NN
cord-007261-b5fgb9wf	125	18	digestion	digestion	NN
cord-007261-b5fgb9wf	125	19	than	than	IN
cord-007261-b5fgb9wf	125	20	that	that	DT
cord-007261-b5fgb9wf	125	21	in	in	IN
cord-007261-b5fgb9wf	125	22	the	the	DT
cord-007261-b5fgb9wf	125	23	cells	cell	NNS
cord-007261-b5fgb9wf	125	24	,	,	,
cord-007261-b5fgb9wf	125	25	although	although	IN
cord-007261-b5fgb9wf	125	26	its	-PRON-	PRP$
cord-007261-b5fgb9wf	125	27	resistancy	resistancy	NN
cord-007261-b5fgb9wf	125	28	was	be	VBD
cord-007261-b5fgb9wf	125	29	not	not	RB
cord-007261-b5fgb9wf	125	30	complete	complete	JJ
cord-007261-b5fgb9wf	125	31	(	(	-LRB-
cord-007261-b5fgb9wf	125	32	Fig	Fig	NNP
cord-007261-b5fgb9wf	125	33	.	.	.
cord-007261-b5fgb9wf	125	34	2A	2a	CD
cord-007261-b5fgb9wf	125	35	,	,	,
cord-007261-b5fgb9wf	125	36	lanes	lane	NNS
cord-007261-b5fgb9wf	125	37	8	8	CD
cord-007261-b5fgb9wf	125	38	and	and	CC
cord-007261-b5fgb9wf	125	39	11	11	CD
cord-007261-b5fgb9wf	125	40	)	)	-RRB-
cord-007261-b5fgb9wf	125	41	.	.	.
cord-007261-b5fgb9wf	126	1	The	the	DT
cord-007261-b5fgb9wf	126	2	partial	partial	JJ
cord-007261-b5fgb9wf	126	3	resistancy	resistancy	NN
cord-007261-b5fgb9wf	126	4	of	of	IN
cord-007261-b5fgb9wf	126	5	the	the	DT
cord-007261-b5fgb9wf	126	6	sugar	sugar	NN
cord-007261-b5fgb9wf	126	7	chains	chain	NNS
cord-007261-b5fgb9wf	126	8	of	of	IN
cord-007261-b5fgb9wf	126	9	the	the	DT
cord-007261-b5fgb9wf	126	10	secreted	secrete	VBN
cord-007261-b5fgb9wf	126	11	Sec	Sec	NNP
cord-007261-b5fgb9wf	126	12	product	product	NN
cord-007261-b5fgb9wf	126	13	to	to	IN
cord-007261-b5fgb9wf	126	14	the	the	DT
cord-007261-b5fgb9wf	126	15	endo	endo	NN
cord-007261-b5fgb9wf	126	16	H	H	NNP
cord-007261-b5fgb9wf	126	17	treatment	treatment	NN
cord-007261-b5fgb9wf	126	18	was	be	VBD
cord-007261-b5fgb9wf	126	19	considered	consider	VBN
cord-007261-b5fgb9wf	126	20	to	to	TO
cord-007261-b5fgb9wf	126	21	be	be	VB
cord-007261-b5fgb9wf	126	22	evidence	evidence	NN
cord-007261-b5fgb9wf	126	23	for	for	IN
cord-007261-b5fgb9wf	126	24	its	-PRON-	PRP$
cord-007261-b5fgb9wf	126	25	transit	transit	NN
cord-007261-b5fgb9wf	126	26	through	through	IN
cord-007261-b5fgb9wf	126	27	the	the	DT
cord-007261-b5fgb9wf	126	28	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	126	29	apparatus	apparatus	NN
cord-007261-b5fgb9wf	126	30	,	,	,
cord-007261-b5fgb9wf	126	31	where	where	WRB
cord-007261-b5fgb9wf	126	32	the	the	DT
cord-007261-b5fgb9wf	126	33	complex	complex	JJ
cord-007261-b5fgb9wf	126	34	type	type	NN
cord-007261-b5fgb9wf	126	35	sugar	sugar	NN
cord-007261-b5fgb9wf	126	36	modifications	modification	NNS
cord-007261-b5fgb9wf	126	37	occur	occur	VBP
cord-007261-b5fgb9wf	126	38	.	.	.
cord-007261-b5fgb9wf	127	1	Taking	take	VBG
cord-007261-b5fgb9wf	127	2	advantage	advantage	NN
cord-007261-b5fgb9wf	127	3	of	of	IN
cord-007261-b5fgb9wf	127	4	this	this	DT
cord-007261-b5fgb9wf	127	5	difference	difference	NN
cord-007261-b5fgb9wf	127	6	in	in	IN
cord-007261-b5fgb9wf	127	7	the	the	DT
cord-007261-b5fgb9wf	127	8	endo	endo	NN
cord-007261-b5fgb9wf	127	9	H	h	NN
cord-007261-b5fgb9wf	127	10	sensitivity	sensitivity	NN
cord-007261-b5fgb9wf	127	11	of	of	IN
cord-007261-b5fgb9wf	127	12	the	the	DT
cord-007261-b5fgb9wf	127	13	reporter	reporter	NN
cord-007261-b5fgb9wf	127	14	protein	protein	NN
cord-007261-b5fgb9wf	127	15	,	,	,
cord-007261-b5fgb9wf	127	16	we	-PRON-	PRP
cord-007261-b5fgb9wf	127	17	examined	examine	VBD
cord-007261-b5fgb9wf	127	18	the	the	DT
cord-007261-b5fgb9wf	127	19	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	127	20	localization	localization	NN
cord-007261-b5fgb9wf	127	21	of	of	IN
cord-007261-b5fgb9wf	127	22	the	the	DT
cord-007261-b5fgb9wf	127	23	expression	expression	NN
cord-007261-b5fgb9wf	127	24	products	product	NNS
cord-007261-b5fgb9wf	127	25	of	of	IN
cord-007261-b5fgb9wf	127	26	Sec	Sec	NNP
cord-007261-b5fgb9wf	127	27	/	/	SYM
cord-007261-b5fgb9wf	127	28	Ml	Ml	NNP
cord-007261-b5fgb9wf	127	29	,	,	,
cord-007261-b5fgb9wf	127	30	Sec/	Sec/	NNP
cord-007261-b5fgb9wf	127	31	Mi	Mi	NNP
cord-007261-b5fgb9wf	127	32	-	-	HYPH
cord-007261-b5fgb9wf	127	33	Pro	Pro	NNP
cord-007261-b5fgb9wf	127	34	,	,	,
cord-007261-b5fgb9wf	127	35	and	and	CC
cord-007261-b5fgb9wf	127	36	Sec	Sec	NNP
cord-007261-b5fgb9wf	127	37	/	/	SYM
cord-007261-b5fgb9wf	127	38	Ml	Ml	NNP
cord-007261-b5fgb9wf	127	39	-	-	HYPH
cord-007261-b5fgb9wf	127	40	TM	TM	NNP
cord-007261-b5fgb9wf	127	41	cDNAs	cdna	VBZ
cord-007261-b5fgb9wf	127	42	in	in	IN
cord-007261-b5fgb9wf	127	43	COS	cos	NN
cord-007261-b5fgb9wf	127	44	cells	cell	NNS
cord-007261-b5fgb9wf	127	45	.	.	.
cord-007261-b5fgb9wf	128	1	When	when	WRB
cord-007261-b5fgb9wf	128	2	the	the	DT
cord-007261-b5fgb9wf	128	3	cDNA	cdna	NN
cord-007261-b5fgb9wf	128	4	coding	code	VBG
cord-007261-b5fgb9wf	128	5	for	for	IN
cord-007261-b5fgb9wf	128	6	the	the	DT
cord-007261-b5fgb9wf	128	7	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	128	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	128	9	-	-	HYPH
cord-007261-b5fgb9wf	128	10	entire	entire	JJ
cord-007261-b5fgb9wf	128	11	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	128	12	)	)	-RRB-
cord-007261-b5fgb9wf	128	13	fusion	fusion	NN
cord-007261-b5fgb9wf	128	14	protein	protein	NN
cord-007261-b5fgb9wf	128	15	(	(	-LRB-
cord-007261-b5fgb9wf	128	16	Sec	Sec	NNP
cord-007261-b5fgb9wf	128	17	/	/	SYM
cord-007261-b5fgb9wf	128	18	Ml	Ml	NNP
cord-007261-b5fgb9wf	128	19	)	)	-RRB-
cord-007261-b5fgb9wf	128	20	was	be	VBD
cord-007261-b5fgb9wf	128	21	transfected	transfecte	VBN
cord-007261-b5fgb9wf	128	22	into	into	IN
cord-007261-b5fgb9wf	128	23	COS	COS	NNP
cord-007261-b5fgb9wf	128	24	cells	cell	NNS
cord-007261-b5fgb9wf	128	25	and	and	CC
cord-007261-b5fgb9wf	128	26	pulse	pulse	NN
cord-007261-b5fgb9wf	128	27	-	-	HYPH
cord-007261-b5fgb9wf	128	28	labeled	label	VBN
cord-007261-b5fgb9wf	128	29	with	with	IN
cord-007261-b5fgb9wf	128	30	[	[	-LRB-
cord-007261-b5fgb9wf	128	31	35	35	CD
cord-007261-b5fgb9wf	128	32	S]methionine	S]methionine	NNP
cord-007261-b5fgb9wf	128	33	for	for	IN
cord-007261-b5fgb9wf	128	34	3h	3h	CD
cord-007261-b5fgb9wf	128	35	,	,	,
cord-007261-b5fgb9wf	128	36	the	the	DT
cord-007261-b5fgb9wf	128	37	radiolabeled	radiolabeled	JJ
cord-007261-b5fgb9wf	128	38	product	product	NN
cord-007261-b5fgb9wf	128	39	was	be	VBD
cord-007261-b5fgb9wf	128	40	detected	detect	VBN
cord-007261-b5fgb9wf	128	41	in	in	IN
cord-007261-b5fgb9wf	128	42	the	the	DT
cord-007261-b5fgb9wf	128	43	cells	cell	NNS
cord-007261-b5fgb9wf	128	44	(	(	-LRB-
cord-007261-b5fgb9wf	128	45	Fig	Fig	NNP
cord-007261-b5fgb9wf	128	46	.	.	.
cord-007261-b5fgb9wf	128	47	2B	2B	NNP
cord-007261-b5fgb9wf	128	48	,	,	,
cord-007261-b5fgb9wf	128	49	lane	lane	NN
cord-007261-b5fgb9wf	128	50	1	1	CD
cord-007261-b5fgb9wf	128	51	)	)	-RRB-
cord-007261-b5fgb9wf	128	52	,	,	,
cord-007261-b5fgb9wf	128	53	but	but	CC
cord-007261-b5fgb9wf	128	54	not	not	RB
cord-007261-b5fgb9wf	128	55	in	in	IN
cord-007261-b5fgb9wf	128	56	the	the	DT
cord-007261-b5fgb9wf	128	57	medium	medium	NN
cord-007261-b5fgb9wf	128	58	(	(	-LRB-
cord-007261-b5fgb9wf	128	59	Fig	Fig	NNP
cord-007261-b5fgb9wf	128	60	.	.	.
cord-007261-b5fgb9wf	128	61	2B	2B	NNP
cord-007261-b5fgb9wf	128	62	,	,	,
cord-007261-b5fgb9wf	128	63	lane	lane	NNP
cord-007261-b5fgb9wf	128	64	7	7	CD
cord-007261-b5fgb9wf	128	65	)	)	-RRB-
cord-007261-b5fgb9wf	128	66	.	.	.
cord-007261-b5fgb9wf	129	1	The	the	DT
cord-007261-b5fgb9wf	129	2	product	product	NN
cord-007261-b5fgb9wf	129	3	remained	remain	VBD
cord-007261-b5fgb9wf	129	4	stably	stably	RB
cord-007261-b5fgb9wf	129	5	in	in	IN
cord-007261-b5fgb9wf	129	6	the	the	DT
cord-007261-b5fgb9wf	129	7	cells	cell	NNS
cord-007261-b5fgb9wf	129	8	even	even	RB
cord-007261-b5fgb9wf	129	9	after	after	IN
cord-007261-b5fgb9wf	129	10	a	a	DT
cord-007261-b5fgb9wf	129	11	3	3	CD
cord-007261-b5fgb9wf	129	12	h	h	NN
cord-007261-b5fgb9wf	129	13	chase	chase	NN
cord-007261-b5fgb9wf	129	14	(	(	-LRB-
cord-007261-b5fgb9wf	129	15	Fig	Fig	NNP
cord-007261-b5fgb9wf	129	16	.	.	.
cord-007261-b5fgb9wf	129	17	2B	2b	CD
cord-007261-b5fgb9wf	129	18	,	,	,
cord-007261-b5fgb9wf	129	19	 	 	_SP
cord-007261-b5fgb9wf	129	20	lane	lane	NNP
cord-007261-b5fgb9wf	129	21	4	4	CD
cord-007261-b5fgb9wf	129	22	)	)	-RRB-
cord-007261-b5fgb9wf	129	23	and	and	CC
cord-007261-b5fgb9wf	129	24	was	be	VBD
cord-007261-b5fgb9wf	129	25	not	not	RB
cord-007261-b5fgb9wf	129	26	secreted	secrete	VBN
cord-007261-b5fgb9wf	129	27	into	into	IN
cord-007261-b5fgb9wf	129	28	the	the	DT
cord-007261-b5fgb9wf	129	29	medium	medium	NN
cord-007261-b5fgb9wf	129	30	(	(	-LRB-
cord-007261-b5fgb9wf	129	31	Fig	Fig	NNP
cord-007261-b5fgb9wf	129	32	.	.	.
cord-007261-b5fgb9wf	129	33	2B	2B	NNP
cord-007261-b5fgb9wf	129	34	,	,	,
cord-007261-b5fgb9wf	129	35	lane	lane	NNP
cord-007261-b5fgb9wf	129	36	 	 	_SP
cord-007261-b5fgb9wf	129	37	8)	8)	NNP
cord-007261-b5fgb9wf	129	38	.	.	.
cord-007261-b5fgb9wf	130	1	The	the	DT
cord-007261-b5fgb9wf	130	2	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	130	3	protein	protein	NN
cord-007261-b5fgb9wf	130	4	in	in	IN
cord-007261-b5fgb9wf	130	5	the	the	DT
cord-007261-b5fgb9wf	130	6	cells	cell	NNS
cord-007261-b5fgb9wf	130	7	remained	remain	VBD
cord-007261-b5fgb9wf	130	8	in	in	IN
cord-007261-b5fgb9wf	130	9	an	an	DT
cord-007261-b5fgb9wf	130	10	entirely	entirely	RB
cord-007261-b5fgb9wf	130	11	endo	endo	NN
cord-007261-b5fgb9wf	130	12	H	h	NN
cord-007261-b5fgb9wf	130	13	-	-	HYPH
cord-007261-b5fgb9wf	130	14	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	130	15	form	form	NN
cord-007261-b5fgb9wf	130	16	during	during	IN
cord-007261-b5fgb9wf	130	17	the	the	DT
cord-007261-b5fgb9wf	130	18	pulse	pulse	NN
cord-007261-b5fgb9wf	130	19	and	and	CC
cord-007261-b5fgb9wf	130	20	chase	chase	NN
cord-007261-b5fgb9wf	130	21	periods	period	NNS
cord-007261-b5fgb9wf	130	22	(	(	-LRB-
cord-007261-b5fgb9wf	130	23	Fig	Fig	NNP
cord-007261-b5fgb9wf	130	24	.	.	.
cord-007261-b5fgb9wf	130	25	2B	2b	CD
cord-007261-b5fgb9wf	130	26	,	,	,
cord-007261-b5fgb9wf	130	27	lanes	lane	NNS
cord-007261-b5fgb9wf	130	28	2	2	CD
cord-007261-b5fgb9wf	130	29	and	and	CC
cord-007261-b5fgb9wf	130	30	5	5	CD
cord-007261-b5fgb9wf	130	31	)	)	-RRB-
cord-007261-b5fgb9wf	130	32	.	.	.
cord-007261-b5fgb9wf	131	1	These	these	DT
cord-007261-b5fgb9wf	131	2	results	result	NNS
cord-007261-b5fgb9wf	131	3	indicated	indicate	VBD
cord-007261-b5fgb9wf	131	4	that	that	IN
cord-007261-b5fgb9wf	131	5	the	the	DT
cord-007261-b5fgb9wf	131	6	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	131	7	)	)	-RRB-
cord-007261-b5fgb9wf	131	8	molecule	molecule	NN
cord-007261-b5fgb9wf	131	9	,	,	,
cord-007261-b5fgb9wf	131	10	when	when	WRB
cord-007261-b5fgb9wf	131	11	fused	fuse	VBN
cord-007261-b5fgb9wf	131	12	to	to	IN
cord-007261-b5fgb9wf	131	13	the	the	DT
cord-007261-b5fgb9wf	131	14	C	c	NN
cord-007261-b5fgb9wf	131	15	-	-	HYPH
cord-007261-b5fgb9wf	131	16	terminus	terminus	NN
cord-007261-b5fgb9wf	131	17	of	of	IN
cord-007261-b5fgb9wf	131	18	the	the	DT
cord-007261-b5fgb9wf	131	19	reporter	reporter	NN
cord-007261-b5fgb9wf	131	20	protein	protein	NN
cord-007261-b5fgb9wf	131	21	,	,	,
cord-007261-b5fgb9wf	131	22	exhibited	exhibit	VBD
cord-007261-b5fgb9wf	131	23	the	the	DT
cord-007261-b5fgb9wf	131	24	ability	ability	NN
cord-007261-b5fgb9wf	131	25	of	of	IN
cord-007261-b5fgb9wf	131	26	retaining	retain	VBG
cord-007261-b5fgb9wf	131	27	the	the	DT
cord-007261-b5fgb9wf	131	28	reporter	reporter	NN
cord-007261-b5fgb9wf	131	29	protein	protein	NN
cord-007261-b5fgb9wf	131	30	in	in	IN
cord-007261-b5fgb9wf	131	31	the	the	DT
cord-007261-b5fgb9wf	131	32	ER	ER	NNP
cord-007261-b5fgb9wf	131	33	.	.	.
cord-007261-b5fgb9wf	132	1	To	to	TO
cord-007261-b5fgb9wf	132	2	identify	identify	VB
cord-007261-b5fgb9wf	132	3	the	the	DT
cord-007261-b5fgb9wf	132	4	region	region	NN
cord-007261-b5fgb9wf	132	5	that	that	WDT
cord-007261-b5fgb9wf	132	6	is	be	VBZ
cord-007261-b5fgb9wf	132	7	responsible	responsible	JJ
cord-007261-b5fgb9wf	132	8	for	for	IN
cord-007261-b5fgb9wf	132	9	the	the	DT
cord-007261-b5fgb9wf	132	10	ER	ER	NNP
cord-007261-b5fgb9wf	132	11	retention	retention	NN
cord-007261-b5fgb9wf	132	12	of	of	IN
cord-007261-b5fgb9wf	132	13	the	the	DT
cord-007261-b5fgb9wf	132	14	reporter	reporter	NN
cord-007261-b5fgb9wf	132	15	protein	protein	NN
cord-007261-b5fgb9wf	132	16	,	,	,
cord-007261-b5fgb9wf	132	17	we	-PRON-	PRP
cord-007261-b5fgb9wf	132	18	constructed	construct	VBD
cord-007261-b5fgb9wf	132	19	cDNAs	cDNAs	NNPS
cord-007261-b5fgb9wf	132	20	in	in	IN
cord-007261-b5fgb9wf	132	21	which	which	WDT
cord-007261-b5fgb9wf	132	22	the	the	DT
cord-007261-b5fgb9wf	132	23	C	C	NNP
cord-007261-b5fgb9wf	132	24	-	-	HYPH
cord-007261-b5fgb9wf	132	25	terminal	terminal	JJ
cord-007261-b5fgb9wf	132	26	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	132	27	portion	portion	NN
cord-007261-b5fgb9wf	132	28	of	of	IN
cord-007261-b5fgb9wf	132	29	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	132	30	)	)	-RRB-
cord-007261-b5fgb9wf	132	31	in	in	IN
cord-007261-b5fgb9wf	132	32	the	the	DT
cord-007261-b5fgb9wf	132	33	fusion	fusion	NN
cord-007261-b5fgb9wf	132	34	protein	protein	NN
cord-007261-b5fgb9wf	132	35	was	be	VBD
cord-007261-b5fgb9wf	132	36	deleted	delete	VBN
cord-007261-b5fgb9wf	132	37	,	,	,
cord-007261-b5fgb9wf	132	38	leaving	leave	VBG
cord-007261-b5fgb9wf	132	39	either	either	CC
cord-007261-b5fgb9wf	132	40	the	the	DT
cord-007261-b5fgb9wf	132	41	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	132	42	domain	domain	NN
cord-007261-b5fgb9wf	132	43	or	or	CC
cord-007261-b5fgb9wf	132	44	both	both	CC
cord-007261-b5fgb9wf	132	45	the	the	DT
cord-007261-b5fgb9wf	132	46	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	132	47	and	and	CC
cord-007261-b5fgb9wf	132	48	proline	proline	NN
cord-007261-b5fgb9wf	132	49	-	-	HYPH
cord-007261-b5fgb9wf	132	50	rich	rich	JJ
cord-007261-b5fgb9wf	132	51	(	(	-LRB-
cord-007261-b5fgb9wf	132	52	PR	PR	NNP
cord-007261-b5fgb9wf	132	53	)	)	-RRB-
cord-007261-b5fgb9wf	132	54	domains	domain	NNS
cord-007261-b5fgb9wf	132	55	,	,	,
cord-007261-b5fgb9wf	132	56	Sec	Sec	NNP
cord-007261-b5fgb9wf	132	57	/	/	SYM
cord-007261-b5fgb9wf	132	58	Ml	Ml	NNP
cord-007261-b5fgb9wf	132	59	-	-	HYPH
cord-007261-b5fgb9wf	132	60	TM	TM	NNP
cord-007261-b5fgb9wf	132	61	(	(	-LRB-
cord-007261-b5fgb9wf	132	62	Fig	Fig	NNP
cord-007261-b5fgb9wf	132	63	.	.	.
cord-007261-b5fgb9wf	132	64	2D	2D	NNP
cord-007261-b5fgb9wf	132	65	)	)	-RRB-
cord-007261-b5fgb9wf	132	66	and	and	CC
cord-007261-b5fgb9wf	132	67	Sec	Sec	NNP
cord-007261-b5fgb9wf	132	68	/	/	SYM
cord-007261-b5fgb9wf	132	69	Ml	Ml	NNP
cord-007261-b5fgb9wf	132	70	-	-	HYPH
cord-007261-b5fgb9wf	132	71	Pro	pro	JJ
cord-007261-b5fgb9wf	132	72	(	(	-LRB-
cord-007261-b5fgb9wf	132	73	Fig	Fig	NNP
cord-007261-b5fgb9wf	132	74	.	.	.
cord-007261-b5fgb9wf	132	75	2C	2C	NNP
cord-007261-b5fgb9wf	132	76	)	)	-RRB-
cord-007261-b5fgb9wf	132	77	,	,	,
cord-007261-b5fgb9wf	132	78	respectively	respectively	RB
cord-007261-b5fgb9wf	132	79	.	.	.
cord-007261-b5fgb9wf	133	1	The	the	DT
cord-007261-b5fgb9wf	133	2	transfection	transfection	NN
cord-007261-b5fgb9wf	133	3	products	product	NNS
cord-007261-b5fgb9wf	133	4	of	of	IN
cord-007261-b5fgb9wf	133	5	these	these	DT
cord-007261-b5fgb9wf	133	6	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	133	7	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	133	8	were	be	VBD
cord-007261-b5fgb9wf	133	9	detected	detect	VBN
cord-007261-b5fgb9wf	133	10	only	only	RB
cord-007261-b5fgb9wf	133	11	in	in	IN
cord-007261-b5fgb9wf	133	12	the	the	DT
cord-007261-b5fgb9wf	133	13	cells	cell	NNS
cord-007261-b5fgb9wf	133	14	,	,	,
cord-007261-b5fgb9wf	133	15	i.e.	i.e.	FW
cord-007261-b5fgb9wf	133	16	,	,	,
cord-007261-b5fgb9wf	133	17	not	not	RB
cord-007261-b5fgb9wf	133	18	in	in	IN
cord-007261-b5fgb9wf	133	19	the	the	DT
cord-007261-b5fgb9wf	133	20	medium	medium	NN
cord-007261-b5fgb9wf	134	1	(	(	-LRB-
cord-007261-b5fgb9wf	134	2	Fig	Fig	NNP
cord-007261-b5fgb9wf	134	3	.	.	NNP
cord-007261-b5fgb9wf	134	4	2	2	CD
cord-007261-b5fgb9wf	134	5	,	,	,
cord-007261-b5fgb9wf	134	6	C	C	NNP
cord-007261-b5fgb9wf	134	7	and	and	CC
cord-007261-b5fgb9wf	134	8	D	D	NNP
cord-007261-b5fgb9wf	134	9	,	,	,
cord-007261-b5fgb9wf	134	10	lanes	lane	NNS
cord-007261-b5fgb9wf	134	11	1	1	CD
cord-007261-b5fgb9wf	134	12	,	,	,
cord-007261-b5fgb9wf	134	13	4	4	CD
cord-007261-b5fgb9wf	134	14	,	,	,
cord-007261-b5fgb9wf	134	15	7	7	CD
cord-007261-b5fgb9wf	134	16	,	,	,
cord-007261-b5fgb9wf	134	17	and	and	CC
cord-007261-b5fgb9wf	134	18	8)	8)	NN
cord-007261-b5fgb9wf	134	19	.	.	.
cord-007261-b5fgb9wf	135	1	Both	both	CC
cord-007261-b5fgb9wf	135	2	the	the	DT
cord-007261-b5fgb9wf	135	3	products	product	NNS
cord-007261-b5fgb9wf	135	4	detected	detect	VBN
cord-007261-b5fgb9wf	135	5	in	in	IN
cord-007261-b5fgb9wf	135	6	the	the	DT
cord-007261-b5fgb9wf	135	7	cells	cell	NNS
cord-007261-b5fgb9wf	135	8	were	be	VBD
cord-007261-b5fgb9wf	135	9	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	135	10	to	to	IN
cord-007261-b5fgb9wf	135	11	endo	endo	FW
cord-007261-b5fgb9wf	135	12	H	h	NN
cord-007261-b5fgb9wf	135	13	digestion	digestion	NN
cord-007261-b5fgb9wf	135	14	throughout	throughout	IN
cord-007261-b5fgb9wf	135	15	the	the	DT
cord-007261-b5fgb9wf	135	16	pulse	pulse	NN
cord-007261-b5fgb9wf	135	17	and	and	CC
cord-007261-b5fgb9wf	135	18	chase	chase	NN
cord-007261-b5fgb9wf	135	19	periods	period	NNS
cord-007261-b5fgb9wf	135	20	(	(	-LRB-
cord-007261-b5fgb9wf	135	21	Fig	Fig	NNP
cord-007261-b5fgb9wf	135	22	.	.	NNP
cord-007261-b5fgb9wf	135	23	2	2	CD
cord-007261-b5fgb9wf	135	24	,	,	,
cord-007261-b5fgb9wf	135	25	C	C	NNP
cord-007261-b5fgb9wf	135	26	and	and	CC
cord-007261-b5fgb9wf	135	27	D	D	NNP
cord-007261-b5fgb9wf	135	28	,	,	,
cord-007261-b5fgb9wf	135	29	lanes	lane	NNS
cord-007261-b5fgb9wf	135	30	2	2	CD
cord-007261-b5fgb9wf	135	31	and	and	CC
cord-007261-b5fgb9wf	135	32	5	5	CD
cord-007261-b5fgb9wf	135	33	)	)	-RRB-
cord-007261-b5fgb9wf	135	34	.	.	.
cord-007261-b5fgb9wf	136	1	These	these	DT
cord-007261-b5fgb9wf	136	2	results	result	NNS
cord-007261-b5fgb9wf	136	3	indicated	indicate	VBD
cord-007261-b5fgb9wf	136	4	that	that	IN
cord-007261-b5fgb9wf	136	5	the	the	DT
cord-007261-b5fgb9wf	136	6	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	136	7	segment	segment	NN
cord-007261-b5fgb9wf	136	8	of	of	IN
cord-007261-b5fgb9wf	136	9	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	136	10	)	)	-RRB-
cord-007261-b5fgb9wf	136	11	was	be	VBD
cord-007261-b5fgb9wf	136	12	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	136	13	for	for	IN
cord-007261-b5fgb9wf	136	14	retention	retention	NN
cord-007261-b5fgb9wf	136	15	of	of	IN
cord-007261-b5fgb9wf	136	16	the	the	DT
cord-007261-b5fgb9wf	136	17	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	136	18	proteins	protein	NNS
cord-007261-b5fgb9wf	136	19	in	in	IN
cord-007261-b5fgb9wf	136	20	the	the	DT
cord-007261-b5fgb9wf	136	21	ER	er	NN
cord-007261-b5fgb9wf	136	22	.	.	.
cord-007261-b5fgb9wf	137	1	The	the	DT
cord-007261-b5fgb9wf	137	2	C	C	NNP
cord-007261-b5fgb9wf	137	3	-	-	HYPH
cord-007261-b5fgb9wf	137	4	terminal	terminal	NN
cord-007261-b5fgb9wf	137	5	flanking	flank	VBG
cord-007261-b5fgb9wf	137	6	region	region	NN
cord-007261-b5fgb9wf	137	7	of	of	IN
cord-007261-b5fgb9wf	137	8	the	the	DT
cord-007261-b5fgb9wf	137	9	transmembrane	transmembrane	NNP
cord-007261-b5fgb9wf	137	10	segment	segment	NN
cord-007261-b5fgb9wf	137	11	of	of	IN
cord-007261-b5fgb9wf	137	12	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	138	1	P450s	P450s	NNP
cord-007261-b5fgb9wf	138	2	contains	contain	VBZ
cord-007261-b5fgb9wf	138	3	clustered	cluster	VBN
cord-007261-b5fgb9wf	138	4	multiple	multiple	JJ
cord-007261-b5fgb9wf	138	5	basic	basic	JJ
cord-007261-b5fgb9wf	138	6	amino	amino	NN
cord-007261-b5fgb9wf	138	7	acid	acid	NN
cord-007261-b5fgb9wf	138	8	residues	residue	NNS
cord-007261-b5fgb9wf	138	9	followed	follow	VBN
cord-007261-b5fgb9wf	138	10	by	by	IN
cord-007261-b5fgb9wf	138	11	proline	proline	NN
cord-007261-b5fgb9wf	138	12	and	and	CC
cord-007261-b5fgb9wf	138	13	glycine	glycine	NN
cord-007261-b5fgb9wf	138	14	clusters	cluster	NNS
cord-007261-b5fgb9wf	138	15	,	,	,
cord-007261-b5fgb9wf	138	16	the	the	DT
cord-007261-b5fgb9wf	138	17	PR	PR	NNP
cord-007261-b5fgb9wf	138	18	region	region	NN
cord-007261-b5fgb9wf	138	19	.	.	.
cord-007261-b5fgb9wf	139	1	The	the	DT
cord-007261-b5fgb9wf	139	2	present	present	JJ
cord-007261-b5fgb9wf	139	3	A.	a.	NN
cord-007261-b5fgb9wf	140	1	However	however	RB
cord-007261-b5fgb9wf	140	2	,	,	,
cord-007261-b5fgb9wf	140	3	there	there	EX
cord-007261-b5fgb9wf	140	4	remained	remain	VBD
cord-007261-b5fgb9wf	140	5	a	a	DT
cord-007261-b5fgb9wf	140	6	possibility	possibility	NN
cord-007261-b5fgb9wf	140	7	that	that	IN
cord-007261-b5fgb9wf	140	8	the	the	DT
cord-007261-b5fgb9wf	140	9	oligosaccharide	oligosaccharide	NN
cord-007261-b5fgb9wf	140	10	chains	chain	NNS
cord-007261-b5fgb9wf	140	11	of	of	IN
cord-007261-b5fgb9wf	140	12	the	the	DT
cord-007261-b5fgb9wf	140	13	reporter	reporter	NN
cord-007261-b5fgb9wf	140	14	protein	protein	NN
cord-007261-b5fgb9wf	140	15	portion	portion	NN
cord-007261-b5fgb9wf	140	16	of	of	IN
cord-007261-b5fgb9wf	140	17	the	the	DT
cord-007261-b5fgb9wf	140	18	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	140	19	proteins	protein	NNS
cord-007261-b5fgb9wf	140	20	had	have	VBD
cord-007261-b5fgb9wf	140	21	lost	lose	VBN
cord-007261-b5fgb9wf	140	22	the	the	DT
cord-007261-b5fgb9wf	140	23	potential	potential	NN
cord-007261-b5fgb9wf	140	24	to	to	TO
cord-007261-b5fgb9wf	140	25	undergo	undergo	VB
cord-007261-b5fgb9wf	140	26	the	the	DT
cord-007261-b5fgb9wf	140	27	complex	complex	JJ
cord-007261-b5fgb9wf	140	28	type	type	NN
cord-007261-b5fgb9wf	140	29	sugar	sugar	NN
cord-007261-b5fgb9wf	140	30	modifications	modification	NNS
cord-007261-b5fgb9wf	140	31	.	.	.
cord-007261-b5fgb9wf	141	1	To	to	TO
cord-007261-b5fgb9wf	141	2	rule	rule	VB
cord-007261-b5fgb9wf	141	3	out	out	RP
cord-007261-b5fgb9wf	141	4	this	this	DT
cord-007261-b5fgb9wf	141	5	possibility	possibility	NN
cord-007261-b5fgb9wf	141	6	we	-PRON-	PRP
cord-007261-b5fgb9wf	141	7	performed	perform	VBD
cord-007261-b5fgb9wf	141	8	Brefeldin	Brefeldin	NNP
cord-007261-b5fgb9wf	141	9	A	A	NNP
cord-007261-b5fgb9wf	141	10	(	(	-LRB-
cord-007261-b5fgb9wf	141	11	BFA	BFA	NNP
cord-007261-b5fgb9wf	141	12	)	)	-RRB-
cord-007261-b5fgb9wf	141	13	treatment	treatment	NN
cord-007261-b5fgb9wf	141	14	of	of	IN
cord-007261-b5fgb9wf	141	15	the	the	DT
cord-007261-b5fgb9wf	141	16	transfected	transfecte	VBN
cord-007261-b5fgb9wf	141	17	cells	cell	NNS
cord-007261-b5fgb9wf	141	18	.	.	.
cord-007261-b5fgb9wf	142	1	BFA	BFA	NNP
cord-007261-b5fgb9wf	142	2	shuts	shut	VBZ
cord-007261-b5fgb9wf	142	3	off	off	RP
cord-007261-b5fgb9wf	142	4	the	the	DT
cord-007261-b5fgb9wf	142	5	anterograde	anterograde	JJ
cord-007261-b5fgb9wf	142	6	membrane	membrane	NN
cord-007261-b5fgb9wf	142	7	traffic	traffic	NN
cord-007261-b5fgb9wf	142	8	of	of	IN
cord-007261-b5fgb9wf	142	9	the	the	DT
cord-007261-b5fgb9wf	142	10	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	142	11	complex	complex	NN
cord-007261-b5fgb9wf	142	12	while	while	IN
cord-007261-b5fgb9wf	142	13	enhancing	enhance	VBG
cord-007261-b5fgb9wf	142	14	the	the	DT
cord-007261-b5fgb9wf	142	15	retrograde	retrograde	JJ
cord-007261-b5fgb9wf	142	16	pathway	pathway	NN
cord-007261-b5fgb9wf	142	17	to	to	IN
cord-007261-b5fgb9wf	142	18	the	the	DT
cord-007261-b5fgb9wf	142	19	ER	ER	NNP
cord-007261-b5fgb9wf	142	20	and	and	CC
cord-007261-b5fgb9wf	142	21	,	,	,
cord-007261-b5fgb9wf	142	22	as	as	IN
cord-007261-b5fgb9wf	142	23	a	a	DT
cord-007261-b5fgb9wf	142	24	result	result	NN
cord-007261-b5fgb9wf	142	25	,	,	,
cord-007261-b5fgb9wf	142	26	causes	cause	VBZ
cord-007261-b5fgb9wf	142	27	disassembly	disassembly	NN
cord-007261-b5fgb9wf	142	28	of	of	IN
cord-007261-b5fgb9wf	142	29	the	the	DT
cord-007261-b5fgb9wf	142	30	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	142	31	apparatus	apparatus	NN
cord-007261-b5fgb9wf	142	32	and	and	CC
cord-007261-b5fgb9wf	142	33	its	-PRON-	PRP$
cord-007261-b5fgb9wf	142	34	mixing	mixing	NN
cord-007261-b5fgb9wf	142	35	with	with	IN
cord-007261-b5fgb9wf	142	36	the	the	DT
cord-007261-b5fgb9wf	142	37	ER	er	NN
cord-007261-b5fgb9wf	142	38	.	.	.
cord-007261-b5fgb9wf	143	1	Therefore	therefore	RB
cord-007261-b5fgb9wf	143	2	,	,	,
cord-007261-b5fgb9wf	143	3	the	the	DT
cord-007261-b5fgb9wf	143	4	high	high	JJ
cord-007261-b5fgb9wf	143	5	mannose	mannose	NN
cord-007261-b5fgb9wf	143	6	type	type	NN
cord-007261-b5fgb9wf	143	7	glycoproteins	glycoprotein	NNS
cord-007261-b5fgb9wf	143	8	retained	retain	VBN
cord-007261-b5fgb9wf	143	9	in	in	IN
cord-007261-b5fgb9wf	143	10	the	the	DT
cord-007261-b5fgb9wf	143	11	ER	ER	NNP
cord-007261-b5fgb9wf	143	12	are	be	VBP
cord-007261-b5fgb9wf	143	13	rapidly	rapidly	RB
cord-007261-b5fgb9wf	143	14	processed	process	VBN
cord-007261-b5fgb9wf	143	15	by	by	IN
cord-007261-b5fgb9wf	143	16	the	the	DT
cord-007261-b5fgb9wf	143	17	cis	cis	NNP
cord-007261-b5fgb9wf	143	18	/	/	SYM
cord-007261-b5fgb9wf	143	19	medial	medial	JJ
cord-007261-b5fgb9wf	143	20	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	143	21	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	143	22	into	into	IN
cord-007261-b5fgb9wf	143	23	complex	complex	JJ
cord-007261-b5fgb9wf	143	24	type	type	NN
cord-007261-b5fgb9wf	143	25	glycoproteins	glycoprotein	NNS
cord-007261-b5fgb9wf	143	26	.	.	.
cord-007261-b5fgb9wf	144	1	As	as	IN
cord-007261-b5fgb9wf	144	2	shown	show	VBN
cord-007261-b5fgb9wf	144	3	in	in	IN
cord-007261-b5fgb9wf	144	4	Fig	Fig	NNP
cord-007261-b5fgb9wf	144	5	.	.	.
cord-007261-b5fgb9wf	145	1	3	3	LS
cord-007261-b5fgb9wf	145	2	,	,	,
cord-007261-b5fgb9wf	145	3	the	the	DT
cord-007261-b5fgb9wf	145	4	transfection	transfection	NN
cord-007261-b5fgb9wf	145	5	products	product	NNS
cord-007261-b5fgb9wf	145	6	of	of	IN
cord-007261-b5fgb9wf	145	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	145	8	/	/	SYM
cord-007261-b5fgb9wf	145	9	Ml	Ml	NNP
cord-007261-b5fgb9wf	145	10	,	,	,
cord-007261-b5fgb9wf	145	11	Sec	Sec	NNP
cord-007261-b5fgb9wf	145	12	/	/	SYM
cord-007261-b5fgb9wf	145	13	Mi	Mi	NNP
cord-007261-b5fgb9wf	145	14	-	-	HYPH
cord-007261-b5fgb9wf	145	15	Pro	Pro	NNP
cord-007261-b5fgb9wf	145	16	,	,	,
cord-007261-b5fgb9wf	145	17	and	and	CC
cord-007261-b5fgb9wf	145	18	Sec	Sec	NNP
cord-007261-b5fgb9wf	145	19	/	/	SYM
cord-007261-b5fgb9wf	145	20	Ml	Ml	NNP
cord-007261-b5fgb9wf	145	21	-	-	HYPH
cord-007261-b5fgb9wf	145	22	TM	TM	NNP
cord-007261-b5fgb9wf	145	23	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	145	24	gained	gain	VBD
cord-007261-b5fgb9wf	145	25	partial	partial	JJ
cord-007261-b5fgb9wf	145	26	resistancy	resistancy	NN
cord-007261-b5fgb9wf	145	27	to	to	IN
cord-007261-b5fgb9wf	145	28	the	the	DT
cord-007261-b5fgb9wf	145	29	endo	endo	NN
cord-007261-b5fgb9wf	145	30	H	h	NN
cord-007261-b5fgb9wf	145	31	treatment	treatment	NN
cord-007261-b5fgb9wf	145	32	when	when	WRB
cord-007261-b5fgb9wf	145	33	BFA	BFA	NNP
cord-007261-b5fgb9wf	145	34	was	be	VBD
cord-007261-b5fgb9wf	145	35	present	present	JJ
cord-007261-b5fgb9wf	145	36	during	during	IN
cord-007261-b5fgb9wf	145	37	the	the	DT
cord-007261-b5fgb9wf	145	38	pulse	pulse	NN
cord-007261-b5fgb9wf	145	39	period	period	NN
cord-007261-b5fgb9wf	145	40	(	(	-LRB-
cord-007261-b5fgb9wf	145	41	Fig	Fig	NNP
cord-007261-b5fgb9wf	145	42	.	.	.
cord-007261-b5fgb9wf	145	43	3	3	CD
cord-007261-b5fgb9wf	145	44	,	,	,
cord-007261-b5fgb9wf	145	45	 	 	_SP
cord-007261-b5fgb9wf	145	46	lanes	lane	NNS
cord-007261-b5fgb9wf	145	47	2	2	CD
cord-007261-b5fgb9wf	145	48	,	,	,
cord-007261-b5fgb9wf	145	49	4	4	CD
cord-007261-b5fgb9wf	145	50	,	,	,
cord-007261-b5fgb9wf	145	51	and	and	CC
cord-007261-b5fgb9wf	145	52	6	6	CD
cord-007261-b5fgb9wf	145	53	)	)	-RRB-
cord-007261-b5fgb9wf	145	54	,	,	,
cord-007261-b5fgb9wf	145	55	excluding	exclude	VBG
cord-007261-b5fgb9wf	145	56	the	the	DT
cord-007261-b5fgb9wf	145	57	above	above	JJ
cord-007261-b5fgb9wf	145	58	possibility	possibility	NN
cord-007261-b5fgb9wf	145	59	.	.	.
cord-007261-b5fgb9wf	146	1	To	to	TO
cord-007261-b5fgb9wf	146	2	define	define	VB
cord-007261-b5fgb9wf	146	3	the	the	DT
cord-007261-b5fgb9wf	146	4	retention	retention	NN
cord-007261-b5fgb9wf	146	5	signal	signal	NN
cord-007261-b5fgb9wf	146	6	in	in	IN
cord-007261-b5fgb9wf	146	7	the	the	DT
cord-007261-b5fgb9wf	146	8	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	146	9	segment	segment	NN
cord-007261-b5fgb9wf	146	10	of	of	IN
cord-007261-b5fgb9wf	146	11	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	146	12	)	)	-RRB-
cord-007261-b5fgb9wf	147	1	further	further	RB
cord-007261-b5fgb9wf	147	2	,	,	,
cord-007261-b5fgb9wf	147	3	we	-PRON-	PRP
cord-007261-b5fgb9wf	147	4	constructed	construct	VBD
cord-007261-b5fgb9wf	147	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	147	6	/	/	SYM
cord-007261-b5fgb9wf	147	7	Ml	Ml	NNP
cord-007261-b5fgb9wf	147	8	-	-	HYPH
cord-007261-b5fgb9wf	147	9	H	H	NNP
cord-007261-b5fgb9wf	147	10	and	and	CC
cord-007261-b5fgb9wf	147	11	Sec	Sec	NNP
cord-007261-b5fgb9wf	147	12	/	/	SYM
cord-007261-b5fgb9wf	147	13	Ml	Ml	NNP
cord-007261-b5fgb9wf	147	14	-	-	HYPH
cord-007261-b5fgb9wf	147	15	Q	Q	NNP
cord-007261-b5fgb9wf	148	1	cDNAs	cDNAs	.
cord-007261-b5fgb9wf	149	1	(	(	-LRB-
cord-007261-b5fgb9wf	149	2	see	see	VB
cord-007261-b5fgb9wf	149	3	Fig	Fig	NNP
cord-007261-b5fgb9wf	149	4	.	.	NNP
cord-007261-b5fgb9wf	149	5	1	1	CD
cord-007261-b5fgb9wf	149	6	)	)	-RRB-
cord-007261-b5fgb9wf	149	7	,	,	,
cord-007261-b5fgb9wf	149	8	in	in	IN
cord-007261-b5fgb9wf	149	9	which	which	WDT
cord-007261-b5fgb9wf	149	10	either	either	CC
cord-007261-b5fgb9wf	149	11	the	the	DT
cord-007261-b5fgb9wf	149	12	N	n	CD
cord-007261-b5fgb9wf	149	13	-	-	HYPH
cord-007261-b5fgb9wf	149	14	terminal	terminal	JJ
cord-007261-b5fgb9wf	149	15	one	one	CD
cord-007261-b5fgb9wf	149	16	-	-	HYPH
cord-007261-b5fgb9wf	149	17	half	half	NN
cord-007261-b5fgb9wf	149	18	or	or	CC
cord-007261-b5fgb9wf	149	19	one	one	CD
cord-007261-b5fgb9wf	149	20	-	-	HYPH
cord-007261-b5fgb9wf	149	21	quarter	quarter	NN
cord-007261-b5fgb9wf	149	22	of	of	IN
cord-007261-b5fgb9wf	149	23	the	the	DT
cord-007261-b5fgb9wf	149	24	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	149	25	segment	segment	NN
cord-007261-b5fgb9wf	149	26	of	of	IN
cord-007261-b5fgb9wf	149	27	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	149	28	)	)	-RRB-
cord-007261-b5fgb9wf	149	29	was	be	VBD
cord-007261-b5fgb9wf	149	30	fused	fuse	VBN
cord-007261-b5fgb9wf	149	31	to	to	IN
cord-007261-b5fgb9wf	149	32	the	the	DT
cord-007261-b5fgb9wf	149	33	C	C	NNP
cord-007261-b5fgb9wf	149	34	-	-	HYPH
cord-007261-b5fgb9wf	149	35	terminus	terminus	NN
cord-007261-b5fgb9wf	149	36	of	of	IN
cord-007261-b5fgb9wf	149	37	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	149	38	Sec	Sec	NNP
cord-007261-b5fgb9wf	149	39	.	.	.
cord-007261-b5fgb9wf	150	1	The	the	DT
cord-007261-b5fgb9wf	150	2	transfection	transfection	NN
cord-007261-b5fgb9wf	150	3	products	product	NNS
cord-007261-b5fgb9wf	150	4	of	of	IN
cord-007261-b5fgb9wf	150	5	these	these	DT
cord-007261-b5fgb9wf	150	6	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	150	7	were	be	VBD
cord-007261-b5fgb9wf	150	8	detected	detect	VBN
cord-007261-b5fgb9wf	150	9	only	only	RB
cord-007261-b5fgb9wf	150	10	in	in	IN
cord-007261-b5fgb9wf	150	11	the	the	DT
cord-007261-b5fgb9wf	150	12	cells	cell	NNS
cord-007261-b5fgb9wf	150	13	,	,	,
cord-007261-b5fgb9wf	150	14	i.e.	i.e.	FW
cord-007261-b5fgb9wf	150	15	,	,	,
cord-007261-b5fgb9wf	150	16	not	not	RB
cord-007261-b5fgb9wf	150	17	in	in	IN
cord-007261-b5fgb9wf	150	18	the	the	DT
cord-007261-b5fgb9wf	150	19	medium	medium	NN
cord-007261-b5fgb9wf	150	20	,	,	,
cord-007261-b5fgb9wf	150	21	after	after	IN
cord-007261-b5fgb9wf	150	22	the	the	DT
cord-007261-b5fgb9wf	150	23	pulse	pulse	NN
cord-007261-b5fgb9wf	150	24	or	or	CC
cord-007261-b5fgb9wf	150	25	the	the	DT
cord-007261-b5fgb9wf	150	26	pulse	pulse	NN
cord-007261-b5fgb9wf	150	27	and	and	CC
cord-007261-b5fgb9wf	150	28	chase	chase	NN
cord-007261-b5fgb9wf	150	29	periods	period	NNS
cord-007261-b5fgb9wf	151	1	(	(	-LRB-
cord-007261-b5fgb9wf	151	2	Fig	Fig	NNP
cord-007261-b5fgb9wf	151	3	.	.	.
cord-007261-b5fgb9wf	151	4	 	 	_SP
cord-007261-b5fgb9wf	152	1	2	2	LS
cord-007261-b5fgb9wf	152	2	,	,	,
cord-007261-b5fgb9wf	152	3	E	E	NNP
cord-007261-b5fgb9wf	152	4	and	and	CC
cord-007261-b5fgb9wf	152	5	F	F	NNP
cord-007261-b5fgb9wf	152	6	,	,	,
cord-007261-b5fgb9wf	152	7	lanes	lane	VBZ
cord-007261-b5fgb9wf	152	8	1	1	CD
cord-007261-b5fgb9wf	152	9	,	,	,
cord-007261-b5fgb9wf	152	10	4	4	CD
cord-007261-b5fgb9wf	152	11	,	,	,
cord-007261-b5fgb9wf	152	12	7	7	CD
cord-007261-b5fgb9wf	152	13	,	,	,
cord-007261-b5fgb9wf	152	14	and	and	CC
cord-007261-b5fgb9wf	152	15	8)	8)	NN
cord-007261-b5fgb9wf	152	16	.	.	.
cord-007261-b5fgb9wf	153	1	The	the	DT
cord-007261-b5fgb9wf	153	2	products	product	NNS
cord-007261-b5fgb9wf	153	3	in	in	IN
cord-007261-b5fgb9wf	153	4	the	the	DT
cord-007261-b5fgb9wf	153	5	cells	cell	NNS
cord-007261-b5fgb9wf	153	6	were	be	VBD
cord-007261-b5fgb9wf	153	7	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	153	8	to	to	IN
cord-007261-b5fgb9wf	153	9	endo	endo	FW
cord-007261-b5fgb9wf	153	10	H	h	NN
cord-007261-b5fgb9wf	153	11	digestion	digestion	NN
cord-007261-b5fgb9wf	153	12	(	(	-LRB-
cord-007261-b5fgb9wf	153	13	Fig	fig	NN
cord-007261-b5fgb9wf	153	14	.	.	.
cord-007261-b5fgb9wf	154	1	2	2	LS
cord-007261-b5fgb9wf	154	2	,	,	,
cord-007261-b5fgb9wf	154	3	E	E	NNP
cord-007261-b5fgb9wf	154	4	and	and	CC
cord-007261-b5fgb9wf	154	5	F	F	NNP
cord-007261-b5fgb9wf	154	6	,	,	,
cord-007261-b5fgb9wf	154	7	lanes	lane	NNS
cord-007261-b5fgb9wf	154	8	2	2	CD
cord-007261-b5fgb9wf	154	9	and	and	CC
cord-007261-b5fgb9wf	154	10	5	5	CD
cord-007261-b5fgb9wf	154	11	)	)	-RRB-
cord-007261-b5fgb9wf	154	12	,	,	,
cord-007261-b5fgb9wf	154	13	again	again	RB
cord-007261-b5fgb9wf	154	14	indicating	indicate	VBG
cord-007261-b5fgb9wf	154	15	that	that	IN
cord-007261-b5fgb9wf	154	16	both	both	DT
cord-007261-b5fgb9wf	154	17	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	154	18	proteins	protein	NNS
cord-007261-b5fgb9wf	154	19	are	be	VBP
cord-007261-b5fgb9wf	154	20	retained	retain	VBN
cord-007261-b5fgb9wf	154	21	in	in	IN
cord-007261-b5fgb9wf	154	22	the	the	DT
cord-007261-b5fgb9wf	154	23	ER	ER	NNP
cord-007261-b5fgb9wf	154	24	.	.	.
cord-007261-b5fgb9wf	155	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	155	2	should	should	MD
cord-007261-b5fgb9wf	155	3	be	be	VB
cord-007261-b5fgb9wf	155	4	noted	note	VBN
cord-007261-b5fgb9wf	155	5	that	that	IN
cord-007261-b5fgb9wf	155	6	the	the	DT
cord-007261-b5fgb9wf	155	7	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	155	8	stability	stability	NN
cord-007261-b5fgb9wf	155	9	of	of	IN
cord-007261-b5fgb9wf	155	10	Sec	Sec	NNP
cord-007261-b5fgb9wf	155	11	/	/	SYM
cord-007261-b5fgb9wf	155	12	Ml	Ml	NNP
cord-007261-b5fgb9wf	155	13	-Q	-Q	NNP
cord-007261-b5fgb9wf	155	14	seemed	seem	VBD
cord-007261-b5fgb9wf	155	15	to	to	TO
cord-007261-b5fgb9wf	155	16	be	be	VB
cord-007261-b5fgb9wf	155	17	lower	low	JJR
cord-007261-b5fgb9wf	155	18	than	than	IN
cord-007261-b5fgb9wf	155	19	that	that	DT
cord-007261-b5fgb9wf	155	20	of	of	IN
cord-007261-b5fgb9wf	155	21	other	other	JJ
cord-007261-b5fgb9wf	155	22	Sec	Sec	NNP
cord-007261-b5fgb9wf	155	23	constructs	construct	NNS
cord-007261-b5fgb9wf	155	24	,	,	,
cord-007261-b5fgb9wf	155	25	since	since	IN
cord-007261-b5fgb9wf	155	26	the	the	DT
cord-007261-b5fgb9wf	155	27	recovery	recovery	NN
cord-007261-b5fgb9wf	155	28	of	of	IN
cord-007261-b5fgb9wf	155	29	the	the	DT
cord-007261-b5fgb9wf	155	30	immunoprecipitation	immunoprecipitation	NN
cord-007261-b5fgb9wf	155	31	products	product	NNS
cord-007261-b5fgb9wf	155	32	decreased	decrease	VBD
cord-007261-b5fgb9wf	155	33	significantly	significantly	RB
cord-007261-b5fgb9wf	155	34	after	after	IN
cord-007261-b5fgb9wf	155	35	a	a	DT
cord-007261-b5fgb9wf	155	36	3	3	CD
cord-007261-b5fgb9wf	155	37	h	h	NN
cord-007261-b5fgb9wf	155	38	chase	chase	NN
cord-007261-b5fgb9wf	155	39	(	(	-LRB-
cord-007261-b5fgb9wf	155	40	compare	compare	VB
cord-007261-b5fgb9wf	155	41	lanes	lane	NNS
cord-007261-b5fgb9wf	155	42	1	1	CD
cord-007261-b5fgb9wf	155	43	and	and	CC
cord-007261-b5fgb9wf	155	44	4	4	CD
cord-007261-b5fgb9wf	155	45	in	in	IN
cord-007261-b5fgb9wf	155	46	Fig	Fig	NNP
cord-007261-b5fgb9wf	155	47	.	.	.
cord-007261-b5fgb9wf	155	48	2F	2F	NNP
cord-007261-b5fgb9wf	155	49	)	)	-RRB-
cord-007261-b5fgb9wf	155	50	.	.	.
cord-007261-b5fgb9wf	156	1	Assessment	assessment	NN
cord-007261-b5fgb9wf	156	2	of	of	IN
cord-007261-b5fgb9wf	156	3	the	the	DT
cord-007261-b5fgb9wf	156	4	Correct	Correct	NNP
cord-007261-b5fgb9wf	156	5	Folding	Folding	NNP
cord-007261-b5fgb9wf	156	6	of	of	IN
cord-007261-b5fgb9wf	156	7	the	the	DT
cord-007261-b5fgb9wf	156	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	156	9	Portion	Portion	NNP
cord-007261-b5fgb9wf	156	10	of	of	IN
cord-007261-b5fgb9wf	156	11	the	the	DT
cord-007261-b5fgb9wf	156	12	Chimeric	Chimeric	NNP
cord-007261-b5fgb9wf	156	13	Proteins	Proteins	NNPS
cord-007261-b5fgb9wf	156	14	,	,	,
cord-007261-b5fgb9wf	156	15	as	as	IN
cord-007261-b5fgb9wf	156	16	Monitored	monitor	VBN
cord-007261-b5fgb9wf	156	17	as	as	IN
cord-007261-b5fgb9wf	156	18	Carboxyesterase	Carboxyesterase	NNP
cord-007261-b5fgb9wf	156	19	Activity	Activity	NNP
cord-007261-b5fgb9wf	156	20	-	-	:
cord-007261-b5fgb9wf	156	21	It	-PRON-	PRP
cord-007261-b5fgb9wf	156	22	is	be	VBZ
cord-007261-b5fgb9wf	156	23	well	well	RB
cord-007261-b5fgb9wf	156	24	known	know	VBN
cord-007261-b5fgb9wf	156	25	that	that	DT
cord-007261-b5fgb9wf	156	26	correct	correct	JJ
cord-007261-b5fgb9wf	156	27	folding	folding	NN
cord-007261-b5fgb9wf	156	28	and	and	CC
cord-007261-b5fgb9wf	156	29	assembly	assembly	NN
cord-007261-b5fgb9wf	156	30	are	be	VBP
cord-007261-b5fgb9wf	156	31	necessary	necessary	JJ
cord-007261-b5fgb9wf	156	32	for	for	IN
cord-007261-b5fgb9wf	156	33	the	the	DT
cord-007261-b5fgb9wf	156	34	efficient	efficient	JJ
cord-007261-b5fgb9wf	156	35	export	export	NN
cord-007261-b5fgb9wf	156	36	of	of	IN
cord-007261-b5fgb9wf	156	37	proteins	protein	NNS
cord-007261-b5fgb9wf	156	38	from	from	IN
cord-007261-b5fgb9wf	156	39	the	the	DT
cord-007261-b5fgb9wf	156	40	ER	ER	NNP
cord-007261-b5fgb9wf	156	41	.	.	.
cord-007261-b5fgb9wf	157	1	Unfolded	unfold	VBN
cord-007261-b5fgb9wf	157	2	or	or	CC
cord-007261-b5fgb9wf	157	3	malfolded	malfolde	VBD
cord-007261-b5fgb9wf	157	4	proteins	protein	NNS
cord-007261-b5fgb9wf	157	5	tend	tend	VBP
cord-007261-b5fgb9wf	157	6	to	to	TO
cord-007261-b5fgb9wf	157	7	bind	bind	VB
cord-007261-b5fgb9wf	157	8	to	to	IN
cord-007261-b5fgb9wf	157	9	the	the	DT
cord-007261-b5fgb9wf	157	10	chaperones	chaperone	NNS
cord-007261-b5fgb9wf	157	11	in	in	IN
cord-007261-b5fgb9wf	157	12	the	the	DT
cord-007261-b5fgb9wf	157	13	ER	er	NN
cord-007261-b5fgb9wf	157	14	or	or	CC
cord-007261-b5fgb9wf	157	15	to	to	TO
cord-007261-b5fgb9wf	157	16	form	form	VB
cord-007261-b5fgb9wf	157	17	aggregates	aggregate	NNS
cord-007261-b5fgb9wf	157	18	and	and	CC
cord-007261-b5fgb9wf	157	19	thereby	thereby	RB
cord-007261-b5fgb9wf	157	20	are	be	VBP
cord-007261-b5fgb9wf	157	21	retained	retain	VBN
cord-007261-b5fgb9wf	157	22	in	in	IN
cord-007261-b5fgb9wf	157	23	the	the	DT
cord-007261-b5fgb9wf	157	24	ER	er	NN
cord-007261-b5fgb9wf	157	25	to	to	TO
cord-007261-b5fgb9wf	157	26	be	be	VB
cord-007261-b5fgb9wf	157	27	degraded	degrade	VBN
cord-007261-b5fgb9wf	157	28	.	.	.
cord-007261-b5fgb9wf	158	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	158	2	is	be	VBZ
cord-007261-b5fgb9wf	158	3	,	,	,
cord-007261-b5fgb9wf	158	4	therefore	therefore	RB
cord-007261-b5fgb9wf	158	5	,	,	,
cord-007261-b5fgb9wf	158	6	necessary	necessary	JJ
cord-007261-b5fgb9wf	158	7	to	to	TO
cord-007261-b5fgb9wf	158	8	know	know	VB
cord-007261-b5fgb9wf	158	9	if	if	IN
cord-007261-b5fgb9wf	158	10	the	the	DT
cord-007261-b5fgb9wf	158	11	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	158	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	158	13	fusion	fusion	NN
cord-007261-b5fgb9wf	158	14	proteins	protein	NNS
cord-007261-b5fgb9wf	158	15	expressed	express	VBN
cord-007261-b5fgb9wf	158	16	in	in	IN
cord-007261-b5fgb9wf	158	17	the	the	DT
cord-007261-b5fgb9wf	158	18	COS	COS	NNP
cord-007261-b5fgb9wf	158	19	cells	cell	NNS
cord-007261-b5fgb9wf	158	20	described	describe	VBN
cord-007261-b5fgb9wf	158	21	above	above	RB
cord-007261-b5fgb9wf	158	22	attained	attain	VBD
cord-007261-b5fgb9wf	158	23	the	the	DT
cord-007261-b5fgb9wf	158	24	correct	correct	JJ
cord-007261-b5fgb9wf	158	25	conformations	conformation	NNS
cord-007261-b5fgb9wf	158	26	or	or	CC
cord-007261-b5fgb9wf	158	27	not	not	RB
cord-007261-b5fgb9wf	158	28	.	.	.
cord-007261-b5fgb9wf	159	1	For	for	IN
cord-007261-b5fgb9wf	159	2	this	this	DT
cord-007261-b5fgb9wf	159	3	purpose	purpose	NN
cord-007261-b5fgb9wf	159	4	,	,	,
cord-007261-b5fgb9wf	159	5	we	-PRON-	PRP
cord-007261-b5fgb9wf	159	6	assayed	assay	VBD
cord-007261-b5fgb9wf	159	7	the	the	DT
cord-007261-b5fgb9wf	159	8	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	159	9	activity	activity	NN
cord-007261-b5fgb9wf	159	10	of	of	IN
cord-007261-b5fgb9wf	159	11	the	the	DT
cord-007261-b5fgb9wf	159	12	reporter	reporter	NN
cord-007261-b5fgb9wf	159	13	molecule	molecule	NN
cord-007261-b5fgb9wf	159	14	,	,	,
cord-007261-b5fgb9wf	159	15	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	159	16	Sec	Sec	NNP
cord-007261-b5fgb9wf	159	17	,	,	,
cord-007261-b5fgb9wf	159	18	and	and	CC
cord-007261-b5fgb9wf	159	19	the	the	DT
cord-007261-b5fgb9wf	159	20	results	result	NNS
cord-007261-b5fgb9wf	159	21	are	be	VBP
cord-007261-b5fgb9wf	159	22	shown	show	VBN
cord-007261-b5fgb9wf	159	23	in	in	IN
cord-007261-b5fgb9wf	159	24	Table	Table	NNP
cord-007261-b5fgb9wf	159	25	I	-PRON-	PRP
cord-007261-b5fgb9wf	159	26	.	.	.
cord-007261-b5fgb9wf	160	1	The	the	DT
cord-007261-b5fgb9wf	160	2	basal	basal	NN
cord-007261-b5fgb9wf	160	3	activity	activity	NN
cord-007261-b5fgb9wf	160	4	of	of	IN
cord-007261-b5fgb9wf	160	5	the	the	DT
cord-007261-b5fgb9wf	160	6	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	160	7	of	of	IN
cord-007261-b5fgb9wf	160	8	the	the	DT
cord-007261-b5fgb9wf	160	9	COS	COS	NNP
cord-007261-b5fgb9wf	160	10	cells	cell	NNS
cord-007261-b5fgb9wf	160	11	was	be	VBD
cord-007261-b5fgb9wf	160	12	about	about	RB
cord-007261-b5fgb9wf	160	13	equal	equal	JJ
cord-007261-b5fgb9wf	160	14	to	to	IN
cord-007261-b5fgb9wf	160	15	the	the	DT
cord-007261-b5fgb9wf	160	16	activity	activity	NN
cord-007261-b5fgb9wf	160	17	of	of	IN
cord-007261-b5fgb9wf	160	18	the	the	DT
cord-007261-b5fgb9wf	160	19	expressed	express	VBN
cord-007261-b5fgb9wf	160	20	Sec	Sec	NNP
cord-007261-b5fgb9wf	160	21	fusion	fusion	NN
cord-007261-b5fgb9wf	160	22	proteins	protein	NNS
cord-007261-b5fgb9wf	160	23	.	.	.
cord-007261-b5fgb9wf	161	1	The	the	DT
cord-007261-b5fgb9wf	161	2	expressed	expressed	JJ
cord-007261-b5fgb9wf	161	3	products	product	NNS
cord-007261-b5fgb9wf	161	4	of	of	IN
cord-007261-b5fgb9wf	161	5	the	the	DT
cord-007261-b5fgb9wf	161	6	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	161	7	of	of	IN
cord-007261-b5fgb9wf	162	1	Sec	Sec	NNP
cord-007261-b5fgb9wf	162	2	/	/	SYM
cord-007261-b5fgb9wf	162	3	Ml	Ml	NNP
cord-007261-b5fgb9wf	162	4	,	,	,
cord-007261-b5fgb9wf	162	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	162	6	/	/	SYM
cord-007261-b5fgb9wf	162	7	Mi	Mi	NNP
cord-007261-b5fgb9wf	162	8	-	-	HYPH
cord-007261-b5fgb9wf	162	9	Pro	Pro	NNP
cord-007261-b5fgb9wf	162	10	,	,	,
cord-007261-b5fgb9wf	162	11	and	and	CC
cord-007261-b5fgb9wf	162	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	162	13	/	/	SYM
cord-007261-b5fgb9wf	162	14	Ml	Ml	NNP
cord-007261-b5fgb9wf	162	15	-	-	HYPH
cord-007261-b5fgb9wf	162	16	TM	TM	NNP
cord-007261-b5fgb9wf	162	17	exhibited	exhibit	VBD
cord-007261-b5fgb9wf	162	18	significant	significant	JJ
cord-007261-b5fgb9wf	162	19	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	162	20	activities	activity	NNS
cord-007261-b5fgb9wf	162	21	.	.	.
cord-007261-b5fgb9wf	163	1	In	in	IN
cord-007261-b5fgb9wf	163	2	contrast	contrast	NN
cord-007261-b5fgb9wf	163	3	,	,	,
cord-007261-b5fgb9wf	163	4	however	however	RB
cord-007261-b5fgb9wf	163	5	,	,	,
cord-007261-b5fgb9wf	163	6	no	no	DT
cord-007261-b5fgb9wf	163	7	activity	activity	NN
cord-007261-b5fgb9wf	163	8	was	be	VBD
cord-007261-b5fgb9wf	163	9	detected	detect	VBN
cord-007261-b5fgb9wf	163	10	for	for	IN
cord-007261-b5fgb9wf	163	11	the	the	DT
cord-007261-b5fgb9wf	163	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	163	13	/	/	SYM
cord-007261-b5fgb9wf	163	14	Ml	Ml	NNP
cord-007261-b5fgb9wf	163	15	-	-	HYPH
cord-007261-b5fgb9wf	163	16	H	H	NNP
cord-007261-b5fgb9wf	163	17	or	or	CC
cord-007261-b5fgb9wf	163	18	Sec	Sec	NNP
cord-007261-b5fgb9wf	163	19	/	/	SYM
cord-007261-b5fgb9wf	163	20	Ml	Ml	NNP
cord-007261-b5fgb9wf	163	21	-	-	HYPH
cord-007261-b5fgb9wf	163	22	Q	Q	NNP
cord-007261-b5fgb9wf	163	23	protein	protein	NN
cord-007261-b5fgb9wf	163	24	.	.	.
cord-007261-b5fgb9wf	164	1	These	these	DT
cord-007261-b5fgb9wf	164	2	results	result	NNS
cord-007261-b5fgb9wf	164	3	indicate	indicate	VBP
cord-007261-b5fgb9wf	164	4	that	that	IN
cord-007261-b5fgb9wf	164	5	the	the	DT
cord-007261-b5fgb9wf	164	6	Sec	Sec	NNP
cord-007261-b5fgb9wf	164	7	/	/	SYM
cord-007261-b5fgb9wf	164	8	Ml	Ml	NNP
cord-007261-b5fgb9wf	164	9	,	,	,
cord-007261-b5fgb9wf	164	10	Sec	Sec	NNP
cord-007261-b5fgb9wf	164	11	/	/	SYM
cord-007261-b5fgb9wf	164	12	Ml	Ml	NNP
cord-007261-b5fgb9wf	164	13	-	-	HYPH
cord-007261-b5fgb9wf	164	14	Pro	Pro	NNP
cord-007261-b5fgb9wf	164	15	,	,	,
cord-007261-b5fgb9wf	164	16	and	and	CC
cord-007261-b5fgb9wf	164	17	Sec	Sec	NNP
cord-007261-b5fgb9wf	164	18	/	/	SYM
cord-007261-b5fgb9wf	164	19	Ml	Ml	NNP
cord-007261-b5fgb9wf	164	20	-	-	HYPH
cord-007261-b5fgb9wf	164	21	TM	TM	NNP
cord-007261-b5fgb9wf	164	22	proteins	protein	NNS
cord-007261-b5fgb9wf	164	23	had	have	VBD
cord-007261-b5fgb9wf	164	24	attained	attain	VBN
cord-007261-b5fgb9wf	164	25	the	the	DT
cord-007261-b5fgb9wf	164	26	correct	correct	JJ
cord-007261-b5fgb9wf	164	27	conformations	conformation	NNS
cord-007261-b5fgb9wf	164	28	in	in	IN
cord-007261-b5fgb9wf	164	29	the	the	DT
cord-007261-b5fgb9wf	164	30	COS	COS	NNP
cord-007261-b5fgb9wf	164	31	cells	cell	NNS
cord-007261-b5fgb9wf	164	32	,	,	,
cord-007261-b5fgb9wf	164	33	whereas	whereas	IN
cord-007261-b5fgb9wf	164	34	the	the	DT
cord-007261-b5fgb9wf	164	35	Sec	Sec	NNP
cord-007261-b5fgb9wf	164	36	/	/	SYM
cord-007261-b5fgb9wf	164	37	Ml	Ml	NNP
cord-007261-b5fgb9wf	164	38	-	-	HYPH
cord-007261-b5fgb9wf	164	39	H	H	NNP
cord-007261-b5fgb9wf	164	40	and	and	CC
cord-007261-b5fgb9wf	164	41	Sec/	Sec/	NNP
cord-007261-b5fgb9wf	164	42	Ml	Ml	NNP
cord-007261-b5fgb9wf	164	43	-	-	HYPH
cord-007261-b5fgb9wf	164	44	Q	Q	NNP
cord-007261-b5fgb9wf	164	45	proteins	protein	NNS
cord-007261-b5fgb9wf	164	46	were	be	VBD
cord-007261-b5fgb9wf	164	47	in	in	IN
cord-007261-b5fgb9wf	164	48	malfolded	malfolded	JJ
cord-007261-b5fgb9wf	164	49	states	state	NNS
cord-007261-b5fgb9wf	164	50	and	and	CC
cord-007261-b5fgb9wf	164	51	therefore	therefore	RB
cord-007261-b5fgb9wf	164	52	retained	retain	VBN
cord-007261-b5fgb9wf	164	53	in	in	IN
cord-007261-b5fgb9wf	164	54	the	the	DT
cord-007261-b5fgb9wf	164	55	ER	er	NN
cord-007261-b5fgb9wf	164	56	,	,	,
cord-007261-b5fgb9wf	164	57	probably	probably	RB
cord-007261-b5fgb9wf	164	58	through	through	IN
cord-007261-b5fgb9wf	164	59	interaction	interaction	NN
cord-007261-b5fgb9wf	164	60	with	with	IN
cord-007261-b5fgb9wf	164	61	the	the	DT
cord-007261-b5fgb9wf	164	62	chaperones	chaperone	NNS
cord-007261-b5fgb9wf	164	63	in	in	IN
cord-007261-b5fgb9wf	164	64	the	the	DT
cord-007261-b5fgb9wf	164	65	ER	er	NN
cord-007261-b5fgb9wf	164	66	or	or	CC
cord-007261-b5fgb9wf	164	67	formation	formation	NN
cord-007261-b5fgb9wf	164	68	of	of	IN
cord-007261-b5fgb9wf	164	69	aggregates	aggregate	NNS
cord-007261-b5fgb9wf	164	70	.	.	.
cord-007261-b5fgb9wf	165	1	We	-PRON-	PRP
cord-007261-b5fgb9wf	165	2	wanted	want	VBD
cord-007261-b5fgb9wf	165	3	to	to	TO
cord-007261-b5fgb9wf	165	4	know	know	VB
cord-007261-b5fgb9wf	165	5	whether	whether	IN
cord-007261-b5fgb9wf	165	6	the	the	DT
cord-007261-b5fgb9wf	165	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	165	8	/	/	SYM
cord-007261-b5fgb9wf	165	9	Ml	Ml	NNP
cord-007261-b5fgb9wf	165	10	-	-	HYPH
cord-007261-b5fgb9wf	165	11	H	H	NNP
cord-007261-b5fgb9wf	165	12	and	and	CC
cord-007261-b5fgb9wf	165	13	Sec	Sec	NNP
cord-007261-b5fgb9wf	165	14	/	/	SYM
cord-007261-b5fgb9wf	165	15	Ml	Ml	NNP
cord-007261-b5fgb9wf	165	16	-	-	HYPH
cord-007261-b5fgb9wf	165	17	Q	q	NN
cord-007261-b5fgb9wf	165	18	proteins	protein	NNS
cord-007261-b5fgb9wf	165	19	were	be	VBD
cord-007261-b5fgb9wf	165	20	firmly	firmly	RB
cord-007261-b5fgb9wf	165	21	anchored	anchor	VBN
cord-007261-b5fgb9wf	165	22	to	to	IN
cord-007261-b5fgb9wf	165	23	the	the	DT
cord-007261-b5fgb9wf	165	24	ER	ER	NNP
cord-007261-b5fgb9wf	165	25	membranes	membrane	NNS
cord-007261-b5fgb9wf	165	26	or	or	CC
cord-007261-b5fgb9wf	165	27	translocated	translocate	VBD
cord-007261-b5fgb9wf	165	28	the	the	DT
cord-007261-b5fgb9wf	165	29	membranes	membrane	NNS
cord-007261-b5fgb9wf	165	30	to	to	TO
cord-007261-b5fgb9wf	165	31	bind	bind	VB
cord-007261-b5fgb9wf	165	32	loosely	loosely	RB
cord-007261-b5fgb9wf	165	33	to	to	IN
cord-007261-b5fgb9wf	165	34	the	the	DT
cord-007261-b5fgb9wf	165	35	luminal	luminal	JJ
cord-007261-b5fgb9wf	165	36	surface	surface	NN
cord-007261-b5fgb9wf	165	37	,	,	,
cord-007261-b5fgb9wf	165	38	since	since	IN
cord-007261-b5fgb9wf	165	39	the	the	DT
cord-007261-b5fgb9wf	165	40	Sec	Sec	NNP
cord-007261-b5fgb9wf	165	41	/	/	SYM
cord-007261-b5fgb9wf	165	42	Ml	Ml	NNP
cord-007261-b5fgb9wf	165	43	-	-	HYPH
cord-007261-b5fgb9wf	165	44	H	H	NNP
cord-007261-b5fgb9wf	165	45	and	and	CC
cord-007261-b5fgb9wf	165	46	Sec	Sec	NNP
cord-007261-b5fgb9wf	165	47	/	/	SYM
cord-007261-b5fgb9wf	165	48	Ml	Ml	NNP
cord-007261-b5fgb9wf	165	49	-	-	HYPH
cord-007261-b5fgb9wf	165	50	Q	Q	NNP
cord-007261-b5fgb9wf	165	51	proteins	protein	NNS
cord-007261-b5fgb9wf	165	52	carried	carry	VBD
cord-007261-b5fgb9wf	165	53	the	the	DT
cord-007261-b5fgb9wf	165	54	shorter	shorter	RBR
cord-007261-b5fgb9wf	165	55	truncated	truncated	JJ
cord-007261-b5fgb9wf	165	56	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	165	57	segments	segment	NNS
cord-007261-b5fgb9wf	165	58	of	of	IN
cord-007261-b5fgb9wf	165	59	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	165	60	)	)	-RRB-
cord-007261-b5fgb9wf	165	61	.	.	.
cord-007261-b5fgb9wf	166	1	The	the	DT
cord-007261-b5fgb9wf	166	2	whole	whole	JJ
cord-007261-b5fgb9wf	166	3	cells	cell	NNS
cord-007261-b5fgb9wf	166	4	expressing	express	VBG
cord-007261-b5fgb9wf	166	5	the	the	DT
cord-007261-b5fgb9wf	166	6	Sec	Sec	NNP
cord-007261-b5fgb9wf	166	7	,	,	,
cord-007261-b5fgb9wf	166	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	166	9	/	/	SYM
cord-007261-b5fgb9wf	166	10	Ml	Ml	NNP
cord-007261-b5fgb9wf	166	11	,	,	,
cord-007261-b5fgb9wf	166	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	166	13	/	/	SYM
cord-007261-b5fgb9wf	166	14	Ml	Ml	NNP
cord-007261-b5fgb9wf	166	15	-	-	HYPH
cord-007261-b5fgb9wf	166	16	H	H	NNP
cord-007261-b5fgb9wf	166	17	,	,	,
cord-007261-b5fgb9wf	166	18	and	and	CC
cord-007261-b5fgb9wf	166	19	Sec	Sec	NNP
cord-007261-b5fgb9wf	166	20	/	/	SYM
cord-007261-b5fgb9wf	166	21	Ml	Ml	NNP
cord-007261-b5fgb9wf	166	22	-	-	HYPH
cord-007261-b5fgb9wf	166	23	Q	Q	NNP
cord-007261-b5fgb9wf	166	24	proteins	protein	NNS
cord-007261-b5fgb9wf	166	25	were	be	VBD
cord-007261-b5fgb9wf	166	26	treated	treat	VBN
cord-007261-b5fgb9wf	166	27	with	with	IN
cord-007261-b5fgb9wf	166	28	100	100	CD
cord-007261-b5fgb9wf	166	29	mM	mm	CD
cord-007261-b5fgb9wf	166	30	sodium	sodium	NN
cord-007261-b5fgb9wf	166	31	carbonate	carbonate	NN
cord-007261-b5fgb9wf	166	32	(	(	-LRB-
cord-007261-b5fgb9wf	166	33	pH	pH	NNP
cord-007261-b5fgb9wf	166	34	11.5	11.5	CD
cord-007261-b5fgb9wf	166	35	)	)	-RRB-
cord-007261-b5fgb9wf	166	36	,	,	,
cord-007261-b5fgb9wf	166	37	which	which	WDT
cord-007261-b5fgb9wf	166	38	extracts	extract	VBZ
cord-007261-b5fgb9wf	166	39	both	both	DT
cord-007261-b5fgb9wf	166	40	peripheral	peripheral	JJ
cord-007261-b5fgb9wf	166	41	membrane	membrane	NN
cord-007261-b5fgb9wf	166	42	proteins	protein	NNS
cord-007261-b5fgb9wf	166	43	and	and	CC
cord-007261-b5fgb9wf	166	44	luminal	luminal	JJ
cord-007261-b5fgb9wf	166	45	proteins	protein	NNS
cord-007261-b5fgb9wf	166	46	,	,	,
cord-007261-b5fgb9wf	166	47	and	and	CC
cord-007261-b5fgb9wf	166	48	the	the	DT
cord-007261-b5fgb9wf	166	49	alkali	alkali	NN
cord-007261-b5fgb9wf	166	50	-	-	HYPH
cord-007261-b5fgb9wf	166	51	soluble	soluble	JJ
cord-007261-b5fgb9wf	166	52	and	and	CC
cord-007261-b5fgb9wf	166	53	-insoluble	-insoluble	JJ
cord-007261-b5fgb9wf	166	54	fractions	fraction	NNS
cord-007261-b5fgb9wf	166	55	were	be	VBD
cord-007261-b5fgb9wf	166	56	examined	examine	VBN
cord-007261-b5fgb9wf	166	57	by	by	IN
cord-007261-b5fgb9wf	166	58	Western	western	JJ
cord-007261-b5fgb9wf	166	59	blotting	blotting	NN
cord-007261-b5fgb9wf	166	60	with	with	IN
cord-007261-b5fgb9wf	166	61	anti	anti	JJ
cord-007261-b5fgb9wf	166	62	-	-	JJ
cord-007261-b5fgb9wf	166	63	Est	est	JJ
cord-007261-b5fgb9wf	166	64	antibodies	antibody	NNS
cord-007261-b5fgb9wf	166	65	.	.	.
cord-007261-b5fgb9wf	167	1	As	as	IN
cord-007261-b5fgb9wf	167	2	shown	show	VBN
cord-007261-b5fgb9wf	167	3	in	in	IN
cord-007261-b5fgb9wf	167	4	Fig	Fig	NNP
cord-007261-b5fgb9wf	167	5	.	.	.
cord-007261-b5fgb9wf	168	1	4	4	LS
cord-007261-b5fgb9wf	168	2	,	,	,
cord-007261-b5fgb9wf	168	3	the	the	DT
cord-007261-b5fgb9wf	168	4	original	original	JJ
cord-007261-b5fgb9wf	168	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	168	6	protein	protein	NN
cord-007261-b5fgb9wf	168	7	was	be	VBD
cord-007261-b5fgb9wf	168	8	efficiently	efficiently	RB
cord-007261-b5fgb9wf	168	9	recovered	recover	VBN
cord-007261-b5fgb9wf	168	10	in	in	IN
cord-007261-b5fgb9wf	168	11	the	the	DT
cord-007261-b5fgb9wf	168	12	alkali	alkali	NN
cord-007261-b5fgb9wf	168	13	-	-	HYPH
cord-007261-b5fgb9wf	168	14	soluble	soluble	JJ
cord-007261-b5fgb9wf	168	15	fraction	fraction	NN
cord-007261-b5fgb9wf	168	16	(	(	-LRB-
cord-007261-b5fgb9wf	168	17	lane	lane	NN
cord-007261-b5fgb9wf	168	18	2	2	CD
cord-007261-b5fgb9wf	168	19	)	)	-RRB-
cord-007261-b5fgb9wf	168	20	,	,	,
cord-007261-b5fgb9wf	168	21	whereas	whereas	IN
cord-007261-b5fgb9wf	168	22	other	other	JJ
cord-007261-b5fgb9wf	168	23	fusion	fusion	NN
cord-007261-b5fgb9wf	168	24	proteins	protein	NNS
cord-007261-b5fgb9wf	168	25	were	be	VBD
cord-007261-b5fgb9wf	168	26	mainly	mainly	RB
cord-007261-b5fgb9wf	168	27	recovered	recover	VBN
cord-007261-b5fgb9wf	168	28	in	in	IN
cord-007261-b5fgb9wf	168	29	the	the	DT
cord-007261-b5fgb9wf	168	30	alkali	alkali	RB
cord-007261-b5fgb9wf	168	31	-	-	HYPH
cord-007261-b5fgb9wf	168	32	insoluble	insoluble	JJ
cord-007261-b5fgb9wf	168	33	membrane	membrane	NN
cord-007261-b5fgb9wf	168	34	fractions	fraction	NNS
cord-007261-b5fgb9wf	168	35	(	(	-LRB-
cord-007261-b5fgb9wf	168	36	Fig	Fig	NNP
cord-007261-b5fgb9wf	168	37	.	.	NNP
cord-007261-b5fgb9wf	168	38	4	4	CD
cord-007261-b5fgb9wf	168	39	,	,	,
cord-007261-b5fgb9wf	168	40	lanes	lane	VBZ
cord-007261-b5fgb9wf	168	41	3	3	CD
cord-007261-b5fgb9wf	168	42	,	,	,
cord-007261-b5fgb9wf	168	43	5	5	CD
cord-007261-b5fgb9wf	168	44	,	,	,
cord-007261-b5fgb9wf	168	45	and	and	CC
cord-007261-b5fgb9wf	168	46	7	7	CD
cord-007261-b5fgb9wf	168	47	)	)	-RRB-
cord-007261-b5fgb9wf	168	48	.	.	.
cord-007261-b5fgb9wf	169	1	These	these	DT
cord-007261-b5fgb9wf	169	2	results	result	NNS
cord-007261-b5fgb9wf	169	3	indicated	indicate	VBD
cord-007261-b5fgb9wf	169	4	that	that	IN
cord-007261-b5fgb9wf	169	5	the	the	DT
cord-007261-b5fgb9wf	169	6	Sec	Sec	NNP
cord-007261-b5fgb9wf	169	7	/	/	SYM
cord-007261-b5fgb9wf	169	8	Ml	Ml	NNP
cord-007261-b5fgb9wf	169	9	-	-	HYPH
cord-007261-b5fgb9wf	169	10	H	H	NNP
cord-007261-b5fgb9wf	169	11	and	and	CC
cord-007261-b5fgb9wf	169	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	169	13	/	/	SYM
cord-007261-b5fgb9wf	169	14	Ml	Ml	NNP
cord-007261-b5fgb9wf	169	15	-	-	HYPH
cord-007261-b5fgb9wf	169	16	Q	Q	NNP
cord-007261-b5fgb9wf	169	17	proteins	protein	NNS
cord-007261-b5fgb9wf	169	18	were	be	VBD
cord-007261-b5fgb9wf	169	19	both	both	DT
cord-007261-b5fgb9wf	169	20	stably	stably	RB
cord-007261-b5fgb9wf	169	21	anchored	anchor	VBN
cord-007261-b5fgb9wf	169	22	to	to	IN
cord-007261-b5fgb9wf	169	23	the	the	DT
cord-007261-b5fgb9wf	169	24	ER	ER	NNP
cord-007261-b5fgb9wf	169	25	membrane	membrane	NN
cord-007261-b5fgb9wf	169	26	,	,	,
cord-007261-b5fgb9wf	169	27	even	even	RB
cord-007261-b5fgb9wf	169	28	though	though	IN
cord-007261-b5fgb9wf	169	29	their	-PRON-	PRP$
cord-007261-b5fgb9wf	169	30	C	c	NN
cord-007261-b5fgb9wf	169	31	-	-	HYPH
cord-007261-b5fgb9wf	169	32	terminal	terminal	NN
cord-007261-b5fgb9wf	169	33	hydrophobic	hydrophobic	JJ
cord-007261-b5fgb9wf	169	34	domains	domain	NNS
cord-007261-b5fgb9wf	169	35	seemed	seem	VBD
cord-007261-b5fgb9wf	169	36	to	to	TO
cord-007261-b5fgb9wf	169	37	be	be	VB
cord-007261-b5fgb9wf	169	38	too	too	RB
cord-007261-b5fgb9wf	169	39	short	short	JJ
cord-007261-b5fgb9wf	169	40	for	for	IN
cord-007261-b5fgb9wf	169	41	stable	stable	JJ
cord-007261-b5fgb9wf	169	42	anchoring	anchoring	NN
cord-007261-b5fgb9wf	169	43	to	to	IN
cord-007261-b5fgb9wf	169	44	the	the	DT
cord-007261-b5fgb9wf	169	45	membrane	membrane	NN
cord-007261-b5fgb9wf	169	46	.	.	.
cord-007261-b5fgb9wf	169	47	'	'	''
cord-007261-b5fgb9wf	170	1	Double	double	JJ
cord-007261-b5fgb9wf	170	2	Lysine	lysine	NN
cord-007261-b5fgb9wf	170	3	Motif	motif	NN
cord-007261-b5fgb9wf	170	4	-	-	HYPH
cord-007261-b5fgb9wf	170	5	Dependent	Dependent	NNP
cord-007261-b5fgb9wf	170	6	Retention	Retention	NNP
cord-007261-b5fgb9wf	170	7	of	of	IN
cord-007261-b5fgb9wf	170	8	a	a	DT
cord-007261-b5fgb9wf	170	9	Carboxyesterase	Carboxyesterase	NNP
cord-007261-b5fgb9wf	170	10	Sec	Sec	NNP
cord-007261-b5fgb9wf	170	11	-	-	HYPH
cord-007261-b5fgb9wf	170	12	GT	GT	NNP
cord-007261-b5fgb9wf	170	13	Fusion	Fusion	NNP
cord-007261-b5fgb9wf	170	14	Protein	Protein	NNP
cord-007261-b5fgb9wf	171	1	-The	-The	NFP
cord-007261-b5fgb9wf	171	2	above	above	IN
cord-007261-b5fgb9wf	171	3	described	describe	VBN
cord-007261-b5fgb9wf	171	4	data	datum	NNS
cord-007261-b5fgb9wf	171	5	strongly	strongly	RB
cord-007261-b5fgb9wf	171	6	suggested	suggest	VBD
cord-007261-b5fgb9wf	171	7	that	that	IN
cord-007261-b5fgb9wf	171	8	the	the	DT
cord-007261-b5fgb9wf	171	9	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	171	10	segment	segment	NN
cord-007261-b5fgb9wf	171	11	of	of	IN
cord-007261-b5fgb9wf	171	12	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	171	13	)	)	-RRB-
cord-007261-b5fgb9wf	171	14	was	be	VBD
cord-007261-b5fgb9wf	171	15	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	171	16	to	to	TO
cord-007261-b5fgb9wf	171	17	retain	retain	VB
cord-007261-b5fgb9wf	171	18	the	the	DT
cord-007261-b5fgb9wf	171	19	reporter	reporter	NN
cord-007261-b5fgb9wf	171	20	protein	protein	NN
cord-007261-b5fgb9wf	171	21	as	as	RB
cord-007261-b5fgb9wf	171	22	well	well	RB
cord-007261-b5fgb9wf	171	23	as	as	IN
cord-007261-b5fgb9wf	171	24	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	171	25	)	)	-RRB-
cord-007261-b5fgb9wf	171	26	itself	-PRON-	PRP
cord-007261-b5fgb9wf	171	27	in	in	IN
cord-007261-b5fgb9wf	171	28	the	the	DT
cord-007261-b5fgb9wf	171	29	ER	ER	NNP
cord-007261-b5fgb9wf	171	30	.	.	.
cord-007261-b5fgb9wf	172	1	However	however	RB
cord-007261-b5fgb9wf	172	2	,	,	,
cord-007261-b5fgb9wf	172	3	there	there	EX
cord-007261-b5fgb9wf	172	4	remains	remain	VBZ
cord-007261-b5fgb9wf	172	5	the	the	DT
cord-007261-b5fgb9wf	172	6	possibility	possibility	NN
cord-007261-b5fgb9wf	172	7	that	that	IN
cord-007261-b5fgb9wf	172	8	the	the	DT
cord-007261-b5fgb9wf	172	9	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	172	10	Sec	Sec	NNP
cord-007261-b5fgb9wf	172	11	portion	portion	NN
cord-007261-b5fgb9wf	172	12	of	of	IN
cord-007261-b5fgb9wf	172	13	the	the	DT
cord-007261-b5fgb9wf	172	14	fusion	fusion	NN
cord-007261-b5fgb9wf	172	15	proteins	protein	NNS
cord-007261-b5fgb9wf	172	16	,	,	,
cord-007261-b5fgb9wf	172	17	when	when	WRB
cord-007261-b5fgb9wf	172	18	expressed	express	VBN
cord-007261-b5fgb9wf	172	19	as	as	IN
cord-007261-b5fgb9wf	172	20	the	the	DT
cord-007261-b5fgb9wf	172	21	membrane	membrane	NN
cord-007261-b5fgb9wf	172	22	bound	bind	VBN
cord-007261-b5fgb9wf	172	23	form	form	NN
cord-007261-b5fgb9wf	172	24	,	,	,
cord-007261-b5fgb9wf	172	25	somehow	somehow	RB
cord-007261-b5fgb9wf	172	26	inhibits	inhibit	VBZ
cord-007261-b5fgb9wf	172	27	the	the	DT
cord-007261-b5fgb9wf	172	28	exit	exit	NN
cord-007261-b5fgb9wf	172	29	of	of	IN
cord-007261-b5fgb9wf	172	30	the	the	DT
cord-007261-b5fgb9wf	172	31	proteins	protein	NNS
cord-007261-b5fgb9wf	172	32	from	from	IN
cord-007261-b5fgb9wf	172	33	the	the	DT
cord-007261-b5fgb9wf	172	34	ER	ER	NNP
cord-007261-b5fgb9wf	172	35	.	.	.
cord-007261-b5fgb9wf	173	1	To	to	TO
cord-007261-b5fgb9wf	173	2	exclude	exclude	VB
cord-007261-b5fgb9wf	173	3	this	this	DT
cord-007261-b5fgb9wf	173	4	possibility	possibility	NN
cord-007261-b5fgb9wf	173	5	,	,	,
cord-007261-b5fgb9wf	173	6	we	-PRON-	PRP
cord-007261-b5fgb9wf	173	7	constructed	construct	VBD
cord-007261-b5fgb9wf	173	8	cDNAs	cDNAs	NNPS
cord-007261-b5fgb9wf	173	9	in	in	IN
cord-007261-b5fgb9wf	173	10	which	which	WDT
cord-007261-b5fgb9wf	173	11	the	the	DT
cord-007261-b5fgb9wf	173	12	P450-derived	p450-derived	JJ
cord-007261-b5fgb9wf	173	13	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	173	14	domain	domain	NN
cord-007261-b5fgb9wf	173	15	of	of	IN
cord-007261-b5fgb9wf	173	16	the	the	DT
cord-007261-b5fgb9wf	173	17	fusion	fusion	NN
cord-007261-b5fgb9wf	173	18	protein	protein	NN
cord-007261-b5fgb9wf	173	19	,	,	,
cord-007261-b5fgb9wf	173	20	Sec	Sec	NNP
cord-007261-b5fgb9wf	173	21	/	/	SYM
cord-007261-b5fgb9wf	173	22	Ml	Ml	NNP
cord-007261-b5fgb9wf	173	23	-	-	HYPH
cord-007261-b5fgb9wf	173	24	TM	TM	NNP
cord-007261-b5fgb9wf	173	25	,	,	,
cord-007261-b5fgb9wf	173	26	was	be	VBD
cord-007261-b5fgb9wf	173	27	replaced	replace	VBN
cord-007261-b5fgb9wf	173	28	either	either	CC
cord-007261-b5fgb9wf	173	29	with	with	IN
cord-007261-b5fgb9wf	173	30	the	the	DT
cord-007261-b5fgb9wf	173	31	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	173	32	domain	domain	NN
cord-007261-b5fgb9wf	173	33	and	and	CC
cord-007261-b5fgb9wf	173	34	the	the	DT
cord-007261-b5fgb9wf	173	35	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	173	36	tail	tail	NN
cord-007261-b5fgb9wf	173	37	of	of	IN
cord-007261-b5fgb9wf	173	38	UDPglucronosyltransferase	UDPglucronosyltransferase	NNP
cord-007261-b5fgb9wf	174	1	(	(	-LRB-
cord-007261-b5fgb9wf	174	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	174	3	/	/	SYM
cord-007261-b5fgb9wf	174	4	GT	GT	NNP
cord-007261-b5fgb9wf	174	5	in	in	IN
cord-007261-b5fgb9wf	174	6	Fig	Fig	NNP
cord-007261-b5fgb9wf	174	7	.	.	NNP
cord-007261-b5fgb9wf	174	8	1	1	CD
cord-007261-b5fgb9wf	174	9	)	)	-RRB-
cord-007261-b5fgb9wf	175	1	or	or	CC
cord-007261-b5fgb9wf	175	2	with	with	IN
cord-007261-b5fgb9wf	175	3	the	the	DT
cord-007261-b5fgb9wf	175	4	same	same	JJ
cord-007261-b5fgb9wf	175	5	region	region	NN
cord-007261-b5fgb9wf	175	6	from	from	IN
cord-007261-b5fgb9wf	175	7	which	which	WDT
cord-007261-b5fgb9wf	175	8	the	the	DT
cord-007261-b5fgb9wf	175	9	C	C	NNP
cord-007261-b5fgb9wf	175	10	-	-	HYPH
cord-007261-b5fgb9wf	175	11	terminal	terminal	NN
cord-007261-b5fgb9wf	175	12	"	"	``
cord-007261-b5fgb9wf	175	13	double	double	JJ
cord-007261-b5fgb9wf	175	14	lysine	lysine	NN
cord-007261-b5fgb9wf	175	15	motif	motif	NN
cord-007261-b5fgb9wf	175	16	,	,	,
cord-007261-b5fgb9wf	175	17	"	"	''
cord-007261-b5fgb9wf	175	18	the	the	DT
cord-007261-b5fgb9wf	175	19	ER	ER	NNP
cord-007261-b5fgb9wf	175	20	retention	retention	NN
cord-007261-b5fgb9wf	175	21	signal	signal	NN
cord-007261-b5fgb9wf	175	22	,	,	,
cord-007261-b5fgb9wf	175	23	had	have	VBD
cord-007261-b5fgb9wf	175	24	been	be	VBN
cord-007261-b5fgb9wf	175	25	deleted	delete	VBN
cord-007261-b5fgb9wf	176	1	(	(	-LRB-
cord-007261-b5fgb9wf	176	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	176	3	/	/	SYM
cord-007261-b5fgb9wf	176	4	GTd	GTd	NNS
cord-007261-b5fgb9wf	176	5	in	in	IN
cord-007261-b5fgb9wf	176	6	Fig	Fig	NNP
cord-007261-b5fgb9wf	176	7	.	.	.
cord-007261-b5fgb9wf	176	8	 	 	_SP
cord-007261-b5fgb9wf	177	1	1	1	LS
cord-007261-b5fgb9wf	177	2	)	)	-RRB-
cord-007261-b5fgb9wf	177	3	.	.	.
cord-007261-b5fgb9wf	178	1	COS	COS	NNP
cord-007261-b5fgb9wf	178	2	cells	cell	NNS
cord-007261-b5fgb9wf	178	3	were	be	VBD
cord-007261-b5fgb9wf	178	4	transfected	transfecte	VBN
cord-007261-b5fgb9wf	178	5	with	with	IN
cord-007261-b5fgb9wf	178	6	these	these	DT
cord-007261-b5fgb9wf	178	7	cDNAs	cdna	NNS
cord-007261-b5fgb9wf	178	8	,	,	,
cord-007261-b5fgb9wf	178	9	and	and	CC
cord-007261-b5fgb9wf	178	10	then	then	RB
cord-007261-b5fgb9wf	178	11	pulse	pulse	VB
cord-007261-b5fgb9wf	178	12	and	and	CC
cord-007261-b5fgb9wf	178	13	chase	chase	NN
cord-007261-b5fgb9wf	178	14	experiments	experiment	NNS
cord-007261-b5fgb9wf	178	15	were	be	VBD
cord-007261-b5fgb9wf	178	16	performed	perform	VBN
cord-007261-b5fgb9wf	178	17	.	.	.
cord-007261-b5fgb9wf	179	1	As	as	IN
cord-007261-b5fgb9wf	179	2	shown	show	VBN
cord-007261-b5fgb9wf	179	3	in	in	IN
cord-007261-b5fgb9wf	179	4	Fig	Fig	NNP
cord-007261-b5fgb9wf	179	5	.	.	.
cord-007261-b5fgb9wf	180	1	2	2	CD
cord-007261-b5fgb9wf	180	2	G	g	NN
cord-007261-b5fgb9wf	180	3	,	,	,
cord-007261-b5fgb9wf	180	4	the	the	DT
cord-007261-b5fgb9wf	180	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	180	6	/	/	SYM
cord-007261-b5fgb9wf	180	7	GT	GT	NNP
cord-007261-b5fgb9wf	180	8	protein	protein	NN
cord-007261-b5fgb9wf	180	9	was	be	VBD
cord-007261-b5fgb9wf	180	10	detected	detect	VBN
cord-007261-b5fgb9wf	180	11	exclusively	exclusively	RB
cord-007261-b5fgb9wf	180	12	in	in	IN
cord-007261-b5fgb9wf	180	13	the	the	DT
cord-007261-b5fgb9wf	180	14	cells	cell	NNS
cord-007261-b5fgb9wf	180	15	(	(	-LRB-
cord-007261-b5fgb9wf	180	16	Fig	fig	NN
cord-007261-b5fgb9wf	180	17	.	.	NN
cord-007261-b5fgb9wf	180	18	2	2	CD
cord-007261-b5fgb9wf	180	19	G	g	NN
cord-007261-b5fgb9wf	180	20	,	,	,
cord-007261-b5fgb9wf	180	21	lanes	lane	NNS
cord-007261-b5fgb9wf	180	22	1	1	CD
cord-007261-b5fgb9wf	180	23	,	,	,
cord-007261-b5fgb9wf	180	24	4	4	CD
cord-007261-b5fgb9wf	180	25	,	,	,
cord-007261-b5fgb9wf	180	26	7	7	CD
cord-007261-b5fgb9wf	180	27	,	,	,
cord-007261-b5fgb9wf	180	28	and	and	CC
cord-007261-b5fgb9wf	180	29	8	8	CD
cord-007261-b5fgb9wf	180	30	)	)	-RRB-
cord-007261-b5fgb9wf	180	31	and	and	CC
cord-007261-b5fgb9wf	180	32	the	the	DT
cord-007261-b5fgb9wf	180	33	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	180	34	moiety	moiety	NN
cord-007261-b5fgb9wf	180	35	of	of	IN
cord-007261-b5fgb9wf	180	36	the	the	DT
cord-007261-b5fgb9wf	180	37	pulse	pulse	NN
cord-007261-b5fgb9wf	180	38	-	-	HYPH
cord-007261-b5fgb9wf	180	39	labeled	label	VBN
cord-007261-b5fgb9wf	180	40	chimeric	chimeric	JJ
cord-007261-b5fgb9wf	180	41	protein	protein	NN
cord-007261-b5fgb9wf	180	42	was	be	VBD
cord-007261-b5fgb9wf	180	43	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	180	44	to	to	IN
cord-007261-b5fgb9wf	180	45	endo	endo	FW
cord-007261-b5fgb9wf	180	46	H	h	NN
cord-007261-b5fgb9wf	180	47	digestion	digestion	NN
cord-007261-b5fgb9wf	180	48	(	(	-LRB-
cord-007261-b5fgb9wf	180	49	Fig	fig	NN
cord-007261-b5fgb9wf	180	50	.	.	NN
cord-007261-b5fgb9wf	180	51	2	2	CD
cord-007261-b5fgb9wf	180	52	G	g	NN
cord-007261-b5fgb9wf	180	53	,	,	,
cord-007261-b5fgb9wf	180	54	lane	lane	NN
cord-007261-b5fgb9wf	180	55	2	2	CD
cord-007261-b5fgb9wf	180	56	)	)	-RRB-
cord-007261-b5fgb9wf	180	57	,	,	,
cord-007261-b5fgb9wf	180	58	and	and	CC
cord-007261-b5fgb9wf	180	59	the	the	DT
cord-007261-b5fgb9wf	180	60	sensitivity	sensitivity	NN
cord-007261-b5fgb9wf	180	61	was	be	VBD
cord-007261-b5fgb9wf	180	62	not	not	RB
cord-007261-b5fgb9wf	180	63	altered	alter	VBN
cord-007261-b5fgb9wf	180	64	even	even	RB
cord-007261-b5fgb9wf	180	65	after	after	IN
cord-007261-b5fgb9wf	180	66	the	the	DT
cord-007261-b5fgb9wf	180	67	chase	chase	NN
cord-007261-b5fgb9wf	180	68	(	(	-LRB-
cord-007261-b5fgb9wf	180	69	Fig	Fig	NNP
cord-007261-b5fgb9wf	180	70	.	.	.
cord-007261-b5fgb9wf	180	71	2	2	CD
cord-007261-b5fgb9wf	180	72	G	g	NN
cord-007261-b5fgb9wf	180	73	,	,	,
cord-007261-b5fgb9wf	180	74	lane	lane	NN
cord-007261-b5fgb9wf	180	75	5	5	CD
cord-007261-b5fgb9wf	180	76	)	)	-RRB-
cord-007261-b5fgb9wf	180	77	,	,	,
cord-007261-b5fgb9wf	180	78	indicating	indicate	VBG
cord-007261-b5fgb9wf	180	79	that	that	IN
cord-007261-b5fgb9wf	180	80	the	the	DT
cord-007261-b5fgb9wf	180	81	double	double	JJ
cord-007261-b5fgb9wf	180	82	-	-	HYPH
cord-007261-b5fgb9wf	180	83	lysine	lysine	NN
cord-007261-b5fgb9wf	180	84	motif	motif	NN
cord-007261-b5fgb9wf	180	85	functioned	function	VBD
cord-007261-b5fgb9wf	180	86	to	to	TO
cord-007261-b5fgb9wf	180	87	retain	retain	VB
cord-007261-b5fgb9wf	180	88	the	the	DT
cord-007261-b5fgb9wf	180	89	Sec	Sec	NNP
cord-007261-b5fgb9wf	180	90	/	/	SYM
cord-007261-b5fgb9wf	180	91	GT	GT	NNP
cord-007261-b5fgb9wf	180	92	protein	protein	NN
cord-007261-b5fgb9wf	180	93	in	in	IN
cord-007261-b5fgb9wf	180	94	the	the	DT
cord-007261-b5fgb9wf	180	95	ER	ER	NNP
cord-007261-b5fgb9wf	180	96	.	.	.
cord-007261-b5fgb9wf	181	1	On	on	IN
cord-007261-b5fgb9wf	181	2	the	the	DT
cord-007261-b5fgb9wf	181	3	other	other	JJ
cord-007261-b5fgb9wf	181	4	hand	hand	NN
cord-007261-b5fgb9wf	181	5	,	,	,
cord-007261-b5fgb9wf	181	6	transfection	transfection	NN
cord-007261-b5fgb9wf	181	7	with	with	IN
cord-007261-b5fgb9wf	181	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	181	9	/	/	SYM
cord-007261-b5fgb9wf	181	10	GTd	gtd	NN
cord-007261-b5fgb9wf	181	11	cDNA	cdna	NN
cord-007261-b5fgb9wf	181	12	gave	give	VBD
cord-007261-b5fgb9wf	181	13	heterogeneous	heterogeneous	JJ
cord-007261-b5fgb9wf	181	14	products	product	NNS
cord-007261-b5fgb9wf	181	15	shortly	shortly	RB
cord-007261-b5fgb9wf	181	16	after	after	IN
cord-007261-b5fgb9wf	181	17	the	the	DT
cord-007261-b5fgb9wf	181	18	pulse	pulse	NN
cord-007261-b5fgb9wf	181	19	-	-	HYPH
cord-007261-b5fgb9wf	181	20	labeling	labeling	NN
cord-007261-b5fgb9wf	181	21	or	or	CC
cord-007261-b5fgb9wf	181	22	after	after	IN
cord-007261-b5fgb9wf	181	23	the	the	DT
cord-007261-b5fgb9wf	181	24	chase	chase	NN
cord-007261-b5fgb9wf	181	25	(	(	-LRB-
cord-007261-b5fgb9wf	181	26	Fig	Fig	NNP
cord-007261-b5fgb9wf	181	27	.	.	.
cord-007261-b5fgb9wf	181	28	2H	2H	NNP
cord-007261-b5fgb9wf	181	29	,	,	,
cord-007261-b5fgb9wf	181	30	 	 	_SP
cord-007261-b5fgb9wf	181	31	lanes	lane	NNS
cord-007261-b5fgb9wf	181	32	1	1	CD
cord-007261-b5fgb9wf	181	33	and	and	CC
cord-007261-b5fgb9wf	181	34	4	4	CD
cord-007261-b5fgb9wf	181	35	)	)	-RRB-
cord-007261-b5fgb9wf	181	36	,	,	,
cord-007261-b5fgb9wf	181	37	whose	whose	WP$
cord-007261-b5fgb9wf	181	38	mobilities	mobility	NNS
cord-007261-b5fgb9wf	181	39	in	in	IN
cord-007261-b5fgb9wf	181	40	the	the	DT
cord-007261-b5fgb9wf	181	41	gel	gel	NN
cord-007261-b5fgb9wf	181	42	were	be	VBD
cord-007261-b5fgb9wf	181	43	obviously	obviously	RB
cord-007261-b5fgb9wf	181	44	lower	low	JJR
cord-007261-b5fgb9wf	181	45	than	than	IN
cord-007261-b5fgb9wf	181	46	that	that	DT
cord-007261-b5fgb9wf	181	47	of	of	IN
cord-007261-b5fgb9wf	181	48	the	the	DT
cord-007261-b5fgb9wf	181	49	in	in	FW
cord-007261-b5fgb9wf	181	50	vitro	vitro	FW
cord-007261-b5fgb9wf	181	51	synthesized	synthesize	VBN
cord-007261-b5fgb9wf	181	52	Sec	Sec	NNP
cord-007261-b5fgb9wf	181	53	/	/	SYM
cord-007261-b5fgb9wf	181	54	GTd	gtd	NN
cord-007261-b5fgb9wf	181	55	protein	protein	NN
cord-007261-b5fgb9wf	181	56	(	(	-LRB-
cord-007261-b5fgb9wf	181	57	Fig	Fig	NNP
cord-007261-b5fgb9wf	181	58	.	.	.
cord-007261-b5fgb9wf	181	59	2H	2H	NNP
cord-007261-b5fgb9wf	181	60	,	,	,
cord-007261-b5fgb9wf	181	61	lanes	lane	VBZ
cord-007261-b5fgb9wf	181	62	3	3	CD
cord-007261-b5fgb9wf	181	63	and	and	CC
cord-007261-b5fgb9wf	181	64	6	6	CD
cord-007261-b5fgb9wf	181	65	)	)	-RRB-
cord-007261-b5fgb9wf	181	66	.	.	.
cord-007261-b5fgb9wf	182	1	Of	of	IN
cord-007261-b5fgb9wf	182	2	particular	particular	JJ
cord-007261-b5fgb9wf	182	3	significance	significance	NN
cord-007261-b5fgb9wf	182	4	was	be	VBD
cord-007261-b5fgb9wf	182	5	the	the	DT
cord-007261-b5fgb9wf	182	6	finding	finding	NN
cord-007261-b5fgb9wf	182	7	that	that	IN
cord-007261-b5fgb9wf	182	8	the	the	DT
cord-007261-b5fgb9wf	182	9	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	182	10	chains	chain	NNS
cord-007261-b5fgb9wf	182	11	of	of	IN
cord-007261-b5fgb9wf	182	12	the	the	DT
cord-007261-b5fgb9wf	182	13	Sec	Sec	NNP
cord-007261-b5fgb9wf	182	14	/	/	SYM
cord-007261-b5fgb9wf	182	15	GTd	gtd	NN
cord-007261-b5fgb9wf	182	16	protein	protein	NN
cord-007261-b5fgb9wf	182	17	gained	gain	VBD
cord-007261-b5fgb9wf	182	18	partial	partial	JJ
cord-007261-b5fgb9wf	182	19	resistance	resistance	NN
cord-007261-b5fgb9wf	182	20	to	to	IN
cord-007261-b5fgb9wf	182	21	endo	endo	FW
cord-007261-b5fgb9wf	182	22	H	H	NNP
cord-007261-b5fgb9wf	182	23	digestion	digestion	NN
cord-007261-b5fgb9wf	182	24	(	(	-LRB-
cord-007261-b5fgb9wf	182	25	Fig	fig	NN
cord-007261-b5fgb9wf	182	26	.	.	.
cord-007261-b5fgb9wf	183	1	2H	2H	NNP
cord-007261-b5fgb9wf	183	2	,	,	,
cord-007261-b5fgb9wf	183	3	lanes	lane	NNS
cord-007261-b5fgb9wf	183	4	2	2	CD
cord-007261-b5fgb9wf	183	5	and	and	CC
cord-007261-b5fgb9wf	183	6	5	5	CD
cord-007261-b5fgb9wf	183	7	)	)	-RRB-
cord-007261-b5fgb9wf	183	8	,	,	,
cord-007261-b5fgb9wf	183	9	clearly	clearly	RB
cord-007261-b5fgb9wf	183	10	indicating	indicate	VBG
cord-007261-b5fgb9wf	183	11	that	that	IN
cord-007261-b5fgb9wf	183	12	the	the	DT
cord-007261-b5fgb9wf	183	13	fusion	fusion	NN
cord-007261-b5fgb9wf	183	14	protein	protein	NN
cord-007261-b5fgb9wf	183	15	was	be	VBD
cord-007261-b5fgb9wf	183	16	exported	export	VBN
cord-007261-b5fgb9wf	183	17	from	from	IN
cord-007261-b5fgb9wf	183	18	the	the	DT
cord-007261-b5fgb9wf	183	19	ER	ER	NNP
cord-007261-b5fgb9wf	183	20	and	and	CC
cord-007261-b5fgb9wf	183	21	its	-PRON-	PRP$
cord-007261-b5fgb9wf	183	22	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	183	23	moiety	moiety	NN
cord-007261-b5fgb9wf	183	24	underwent	undergo	VBD
cord-007261-b5fgb9wf	183	25	the	the	DT
cord-007261-b5fgb9wf	183	26	complex	complex	JJ
cord-007261-b5fgb9wf	183	27	type	type	NN
cord-007261-b5fgb9wf	183	28	sugar	sugar	NN
cord-007261-b5fgb9wf	183	29	modifications	modification	NNS
cord-007261-b5fgb9wf	183	30	during	during	IN
cord-007261-b5fgb9wf	183	31	the	the	DT
cord-007261-b5fgb9wf	183	32	transit	transit	NN
cord-007261-b5fgb9wf	183	33	through	through	IN
cord-007261-b5fgb9wf	183	34	the	the	DT
cord-007261-b5fgb9wf	183	35	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	183	36	apparatus	apparatus	NN
cord-007261-b5fgb9wf	183	37	.	.	.
cord-007261-b5fgb9wf	184	1	These	these	DT
cord-007261-b5fgb9wf	184	2	results	result	NNS
cord-007261-b5fgb9wf	184	3	indicate	indicate	VBP
cord-007261-b5fgb9wf	184	4	that	that	IN
cord-007261-b5fgb9wf	184	5	the	the	DT
cord-007261-b5fgb9wf	184	6	Sec	Sec	NNP
cord-007261-b5fgb9wf	184	7	portion	portion	NN
cord-007261-b5fgb9wf	184	8	of	of	IN
cord-007261-b5fgb9wf	184	9	the	the	DT
cord-007261-b5fgb9wf	184	10	fusion	fusion	NN
cord-007261-b5fgb9wf	184	11	proteins	protein	NNS
cord-007261-b5fgb9wf	184	12	did	do	VBD
cord-007261-b5fgb9wf	184	13	not	not	RB
cord-007261-b5fgb9wf	184	14	prevent	prevent	VB
cord-007261-b5fgb9wf	184	15	the	the	DT
cord-007261-b5fgb9wf	184	16	proteins	protein	NNS
cord-007261-b5fgb9wf	184	17	from	from	IN
cord-007261-b5fgb9wf	184	18	being	be	VBG
cord-007261-b5fgb9wf	184	19	transported	transport	VBN
cord-007261-b5fgb9wf	184	20	out	out	IN
cord-007261-b5fgb9wf	184	21	of	of	IN
cord-007261-b5fgb9wf	184	22	the	the	DT
cord-007261-b5fgb9wf	184	23	ER	ER	NNP
cord-007261-b5fgb9wf	184	24	.	.	.
cord-007261-b5fgb9wf	185	1	To	to	TO
cord-007261-b5fgb9wf	185	2	determine	determine	VB
cord-007261-b5fgb9wf	185	3	the	the	DT
cord-007261-b5fgb9wf	185	4	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	185	5	localization	localization	NN
cord-007261-b5fgb9wf	185	6	of	of	IN
cord-007261-b5fgb9wf	185	7	the	the	DT
cord-007261-b5fgb9wf	185	8	Sec	Sec	NNP
cord-007261-b5fgb9wf	185	9	/	/	SYM
cord-007261-b5fgb9wf	185	10	GT	GT	NNP
cord-007261-b5fgb9wf	185	11	and	and	CC
cord-007261-b5fgb9wf	185	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	185	13	/	/	SYM
cord-007261-b5fgb9wf	185	14	GTd	GTd	NNS
cord-007261-b5fgb9wf	185	15	proteins	protein	NNS
cord-007261-b5fgb9wf	185	16	in	in	IN
cord-007261-b5fgb9wf	185	17	the	the	DT
cord-007261-b5fgb9wf	185	18	transfected	transfecte	VBN
cord-007261-b5fgb9wf	185	19	cells	cell	NNS
cord-007261-b5fgb9wf	185	20	,	,	,
cord-007261-b5fgb9wf	185	21	we	-PRON-	PRP
cord-007261-b5fgb9wf	185	22	fractionated	fractionate	VBD
cord-007261-b5fgb9wf	185	23	the	the	DT
cord-007261-b5fgb9wf	185	24	cell	cell	NN
cord-007261-b5fgb9wf	185	25	lysate	lysate	NN
cord-007261-b5fgb9wf	185	26	by	by	IN
cord-007261-b5fgb9wf	185	27	sucrose	sucrose	NN
cord-007261-b5fgb9wf	185	28	density	density	NN
cord-007261-b5fgb9wf	185	29	gradient	gradient	NN
cord-007261-b5fgb9wf	185	30	centrifugation	centrifugation	NN
cord-007261-b5fgb9wf	185	31	,	,	,
cord-007261-b5fgb9wf	185	32	followed	follow	VBN
cord-007261-b5fgb9wf	185	33	by	by	IN
cord-007261-b5fgb9wf	185	34	immunochemical	immunochemical	JJ
cord-007261-b5fgb9wf	185	35	detection	detection	NN
cord-007261-b5fgb9wf	185	36	of	of	IN
cord-007261-b5fgb9wf	185	37	the	the	DT
cord-007261-b5fgb9wf	185	38	fusion	fusion	NN
cord-007261-b5fgb9wf	185	39	proteins	protein	NNS
cord-007261-b5fgb9wf	185	40	by	by	IN
cord-007261-b5fgb9wf	185	41	Western	western	JJ
cord-007261-b5fgb9wf	185	42	blotting	blotting	NN
cord-007261-b5fgb9wf	185	43	(	(	-LRB-
cord-007261-b5fgb9wf	185	44	Fig	Fig	NNP
cord-007261-b5fgb9wf	185	45	.	.	NNP
cord-007261-b5fgb9wf	185	46	5	5	CD
cord-007261-b5fgb9wf	185	47	,	,	,
cord-007261-b5fgb9wf	185	48	B	b	NN
cord-007261-b5fgb9wf	185	49	and	and	CC
cord-007261-b5fgb9wf	185	50	C	C	NNP
cord-007261-b5fgb9wf	185	51	)	)	-RRB-
cord-007261-b5fgb9wf	185	52	.	.	.
cord-007261-b5fgb9wf	186	1	Each	each	DT
cord-007261-b5fgb9wf	186	2	of	of	IN
cord-007261-b5fgb9wf	186	3	the	the	DT
cord-007261-b5fgb9wf	186	4	fractions	fraction	NNS
cord-007261-b5fgb9wf	186	5	was	be	VBD
cord-007261-b5fgb9wf	186	6	also	also	RB
cord-007261-b5fgb9wf	186	7	assayed	assay	VBN
cord-007261-b5fgb9wf	186	8	for	for	IN
cord-007261-b5fgb9wf	186	9	NADPH	nadph	NN
cord-007261-b5fgb9wf	186	10	-	-	HYPH
cord-007261-b5fgb9wf	186	11	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	186	12	c	c	NN
cord-007261-b5fgb9wf	186	13	reductase	reductase	NN
cord-007261-b5fgb9wf	186	14	and	and	CC
cord-007261-b5fgb9wf	186	15	a	a	DT
cord-007261-b5fgb9wf	186	16	-mannosidase	-mannosidase	:
cord-007261-b5fgb9wf	186	17	II	II	NNP
cord-007261-b5fgb9wf	186	18	activities	activity	NNS
cord-007261-b5fgb9wf	186	19	to	to	TO
cord-007261-b5fgb9wf	186	20	locate	locate	VB
cord-007261-b5fgb9wf	186	21	the	the	DT
cord-007261-b5fgb9wf	186	22	ER	ER	NNP
cord-007261-b5fgb9wf	186	23	and	and	CC
cord-007261-b5fgb9wf	186	24	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	186	25	membranes	membrane	NNS
cord-007261-b5fgb9wf	186	26	,	,	,
cord-007261-b5fgb9wf	186	27	respectively	respectively	RB
cord-007261-b5fgb9wf	186	28	(	(	-LRB-
cord-007261-b5fgb9wf	186	29	Fig	fig	NN
cord-007261-b5fgb9wf	186	30	.	.	.
cord-007261-b5fgb9wf	186	31	5A	5a	CD
cord-007261-b5fgb9wf	186	32	)	)	-RRB-
cord-007261-b5fgb9wf	186	33	.	.	.
cord-007261-b5fgb9wf	187	1	The	the	DT
cord-007261-b5fgb9wf	187	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	187	3	/	/	SYM
cord-007261-b5fgb9wf	187	4	GT	GT	NNP
cord-007261-b5fgb9wf	187	5	protein	protein	NN
cord-007261-b5fgb9wf	187	6	was	be	VBD
cord-007261-b5fgb9wf	187	7	detected	detect	VBN
cord-007261-b5fgb9wf	187	8	in	in	IN
cord-007261-b5fgb9wf	187	9	fractions	fraction	NNS
cord-007261-b5fgb9wf	187	10	5	5	CD
cord-007261-b5fgb9wf	187	11	and	and	CC
cord-007261-b5fgb9wf	187	12	6	6	CD
cord-007261-b5fgb9wf	187	13	,	,	,
cord-007261-b5fgb9wf	187	14	which	which	WDT
cord-007261-b5fgb9wf	187	15	was	be	VBD
cord-007261-b5fgb9wf	187	16	coincident	coincident	JJ
cord-007261-b5fgb9wf	187	17	with	with	IN
cord-007261-b5fgb9wf	187	18	NADPH	nadph	NN
cord-007261-b5fgb9wf	187	19	-	-	HYPH
cord-007261-b5fgb9wf	187	20	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	187	21	c	c	NN
cord-007261-b5fgb9wf	187	22	reductase	reductase	NN
cord-007261-b5fgb9wf	187	23	activity	activity	NN
cord-007261-b5fgb9wf	187	24	,	,	,
cord-007261-b5fgb9wf	187	25	but	but	CC
cord-007261-b5fgb9wf	187	26	not	not	RB
cord-007261-b5fgb9wf	187	27	with	with	IN
cord-007261-b5fgb9wf	187	28	that	that	DT
cord-007261-b5fgb9wf	187	29	of	of	IN
cord-007261-b5fgb9wf	187	30	a	a	DT
cord-007261-b5fgb9wf	187	31	-mannosidase	-mannosidase	NNP
cord-007261-b5fgb9wf	187	32	II	II	NNP
cord-007261-b5fgb9wf	187	33	(	(	-LRB-
cord-007261-b5fgb9wf	187	34	Fig	Fig	NNP
cord-007261-b5fgb9wf	187	35	.	.	.
cord-007261-b5fgb9wf	187	36	5B	5b	CD
cord-007261-b5fgb9wf	187	37	)	)	-RRB-
cord-007261-b5fgb9wf	187	38	.	.	.
cord-007261-b5fgb9wf	188	1	In	in	IN
cord-007261-b5fgb9wf	188	2	marked	marked	JJ
cord-007261-b5fgb9wf	188	3	contrast	contrast	NN
cord-007261-b5fgb9wf	188	4	,	,	,
cord-007261-b5fgb9wf	188	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	188	6	/	/	SYM
cord-007261-b5fgb9wf	188	7	GTd	GTd	NNS
cord-007261-b5fgb9wf	188	8	exhibited	exhibit	VBD
cord-007261-b5fgb9wf	188	9	a	a	DT
cord-007261-b5fgb9wf	188	10	diffuse	diffuse	JJ
cord-007261-b5fgb9wf	188	11	distribution	distribution	NN
cord-007261-b5fgb9wf	188	12	,	,	,
cord-007261-b5fgb9wf	188	13	but	but	CC
cord-007261-b5fgb9wf	188	14	was	be	VBD
cord-007261-b5fgb9wf	188	15	mainly	mainly	RB
cord-007261-b5fgb9wf	188	16	detected	detect	VBN
cord-007261-b5fgb9wf	188	17	in	in	IN
cord-007261-b5fgb9wf	188	18	fractions	fraction	NNS
cord-007261-b5fgb9wf	188	19	2	2	CD
cord-007261-b5fgb9wf	188	20	,	,	,
cord-007261-b5fgb9wf	188	21	3	3	CD
cord-007261-b5fgb9wf	188	22	,	,	,
cord-007261-b5fgb9wf	188	23	and	and	CC
cord-007261-b5fgb9wf	188	24	4	4	CD
cord-007261-b5fgb9wf	188	25	,	,	,
cord-007261-b5fgb9wf	188	26	where	where	WRB
cord-007261-b5fgb9wf	188	27	the	the	DT
cord-007261-b5fgb9wf	188	28	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	188	29	marker	marker	NN
cord-007261-b5fgb9wf	188	30	enzyme	enzyme	NN
cord-007261-b5fgb9wf	188	31	,	,	,
cord-007261-b5fgb9wf	188	32	a	a	DT
cord-007261-b5fgb9wf	188	33	-mannosidase	-mannosidase	NNP
cord-007261-b5fgb9wf	188	34	II	II	NNP
cord-007261-b5fgb9wf	188	35	,	,	,
cord-007261-b5fgb9wf	188	36	was	be	VBD
cord-007261-b5fgb9wf	188	37	present	present	JJ
cord-007261-b5fgb9wf	188	38	,	,	,
cord-007261-b5fgb9wf	188	39	although	although	IN
cord-007261-b5fgb9wf	188	40	a	a	DT
cord-007261-b5fgb9wf	188	41	small	small	JJ
cord-007261-b5fgb9wf	188	42	amount	amount	NN
cord-007261-b5fgb9wf	188	43	was	be	VBD
cord-007261-b5fgb9wf	188	44	present	present	JJ
cord-007261-b5fgb9wf	188	45	in	in	IN
cord-007261-b5fgb9wf	188	46	the	the	DT
cord-007261-b5fgb9wf	188	47	ER	ER	NNP
cord-007261-b5fgb9wf	188	48	fractions	fraction	NNS
cord-007261-b5fgb9wf	188	49	(	(	-LRB-
cord-007261-b5fgb9wf	188	50	Fig	Fig	NNP
cord-007261-b5fgb9wf	188	51	.	.	.
cord-007261-b5fgb9wf	188	52	5C	5c	CD
cord-007261-b5fgb9wf	188	53	)	)	-RRB-
cord-007261-b5fgb9wf	188	54	.	.	.
cord-007261-b5fgb9wf	189	1	These	these	DT
cord-007261-b5fgb9wf	189	2	results	result	NNS
cord-007261-b5fgb9wf	189	3	confirmed	confirm	VBD
cord-007261-b5fgb9wf	189	4	that	that	IN
cord-007261-b5fgb9wf	189	5	the	the	DT
cord-007261-b5fgb9wf	189	6	Sec	Sec	NNP
cord-007261-b5fgb9wf	189	7	portion	portion	NN
cord-007261-b5fgb9wf	189	8	of	of	IN
cord-007261-b5fgb9wf	189	9	the	the	DT
cord-007261-b5fgb9wf	189	10	fusion	fusion	NN
cord-007261-b5fgb9wf	189	11	proteins	protein	NNS
cord-007261-b5fgb9wf	189	12	did	do	VBD
cord-007261-b5fgb9wf	189	13	not	not	RB
cord-007261-b5fgb9wf	189	14	prevent	prevent	VB
cord-007261-b5fgb9wf	189	15	the	the	DT
cord-007261-b5fgb9wf	189	16	export	export	NN
cord-007261-b5fgb9wf	189	17	of	of	IN
cord-007261-b5fgb9wf	189	18	the	the	DT
cord-007261-b5fgb9wf	189	19	proteins	protein	NNS
cord-007261-b5fgb9wf	189	20	from	from	IN
cord-007261-b5fgb9wf	189	21	the	the	DT
cord-007261-b5fgb9wf	189	22	ER	ER	NNP
cord-007261-b5fgb9wf	189	23	,	,	,
cord-007261-b5fgb9wf	189	24	providing	provide	VBG
cord-007261-b5fgb9wf	189	25	further	further	JJ
cord-007261-b5fgb9wf	189	26	support	support	NN
cord-007261-b5fgb9wf	189	27	for	for	IN
cord-007261-b5fgb9wf	189	28	the	the	DT
cord-007261-b5fgb9wf	189	29	conclusion	conclusion	NN
cord-007261-b5fgb9wf	189	30	that	that	IN
cord-007261-b5fgb9wf	189	31	the	the	DT
cord-007261-b5fgb9wf	189	32	transmembrane	transmembrane	NNP
cord-007261-b5fgb9wf	189	33	segment	segment	NN
cord-007261-b5fgb9wf	189	34	of	of	IN
cord-007261-b5fgb9wf	189	35	P450(Ml	P450(Ml	NNP
cord-007261-b5fgb9wf	189	36	)	)	-RRB-
cord-007261-b5fgb9wf	189	37	was	be	VBD
cord-007261-b5fgb9wf	189	38	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	189	39	for	for	IN
cord-007261-b5fgb9wf	189	40	the	the	DT
cord-007261-b5fgb9wf	189	41	retention	retention	NN
cord-007261-b5fgb9wf	189	42	in	in	IN
cord-007261-b5fgb9wf	189	43	the	the	DT
cord-007261-b5fgb9wf	189	44	ER	ER	NNP
cord-007261-b5fgb9wf	189	45	.	.	.
cord-007261-b5fgb9wf	190	1	Intracellular	Intracellular	NNP
cord-007261-b5fgb9wf	190	2	Localization	Localization	NNP
cord-007261-b5fgb9wf	190	3	of	of	IN
cord-007261-b5fgb9wf	190	4	Carboxyesterase	Carboxyesterase	NNP
cord-007261-b5fgb9wf	190	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	190	6	Fusion	Fusion	NNP
cord-007261-b5fgb9wf	190	7	Proteins	protein	NNS
cord-007261-b5fgb9wf	190	8	Detected	detect	VBN
cord-007261-b5fgb9wf	190	9	by	by	IN
cord-007261-b5fgb9wf	190	10	Immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	190	11	Microscopy-	microscopy-	RB
cord-007261-b5fgb9wf	191	1	In	in	IN
cord-007261-b5fgb9wf	191	2	order	order	NN
cord-007261-b5fgb9wf	191	3	to	to	TO
cord-007261-b5fgb9wf	191	4	confirm	confirm	VB
cord-007261-b5fgb9wf	191	5	the	the	DT
cord-007261-b5fgb9wf	191	6	above	above	JJ
cord-007261-b5fgb9wf	191	7	biochemical	biochemical	JJ
cord-007261-b5fgb9wf	191	8	observations	observation	NNS
cord-007261-b5fgb9wf	191	9	morphologically	morphologically	RB
cord-007261-b5fgb9wf	191	10	,	,	,
cord-007261-b5fgb9wf	191	11	we	-PRON-	PRP
cord-007261-b5fgb9wf	191	12	next	next	RB
cord-007261-b5fgb9wf	191	13	compared	compare	VBD
cord-007261-b5fgb9wf	191	14	the	the	DT
cord-007261-b5fgb9wf	191	15	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	191	16	localization	localization	NN
cord-007261-b5fgb9wf	191	17	of	of	IN
cord-007261-b5fgb9wf	191	18	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	191	19	)	)	-RRB-
cord-007261-b5fgb9wf	191	20	and	and	CC
cord-007261-b5fgb9wf	191	21	various	various	JJ
cord-007261-b5fgb9wf	191	22	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	191	23	Sec	Sec	NNP
cord-007261-b5fgb9wf	191	24	fusion	fusion	NN
cord-007261-b5fgb9wf	191	25	proteins	protein	NNS
cord-007261-b5fgb9wf	191	26	by	by	IN
cord-007261-b5fgb9wf	191	27	immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	191	28	microscopy	microscopy	NN
cord-007261-b5fgb9wf	191	29	.	.	.
cord-007261-b5fgb9wf	192	1	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	192	2	)	)	-RRB-
cord-007261-b5fgb9wf	193	1	expressed	express	VBN
cord-007261-b5fgb9wf	193	2	in	in	IN
cord-007261-b5fgb9wf	193	3	COS	COS	NNP
cord-007261-b5fgb9wf	193	4	cells	cell	NNS
cord-007261-b5fgb9wf	194	1	showed	show	VBD
cord-007261-b5fgb9wf	194	2	a	a	DT
cord-007261-b5fgb9wf	194	3	tubular	tubular	JJ
cord-007261-b5fgb9wf	194	4	network	network	NN
cord-007261-b5fgb9wf	194	5	extending	extend	VBG
cord-007261-b5fgb9wf	194	6	throughout	throughout	IN
cord-007261-b5fgb9wf	194	7	the	the	DT
cord-007261-b5fgb9wf	194	8	cytoplasm	cytoplasm	NN
cord-007261-b5fgb9wf	194	9	,	,	,
cord-007261-b5fgb9wf	194	10	the	the	DT
cord-007261-b5fgb9wf	194	11	typical	typical	JJ
cord-007261-b5fgb9wf	194	12	ER	ER	NNP
cord-007261-b5fgb9wf	194	13	pattern	pattern	NN
cord-007261-b5fgb9wf	194	14	(	(	-LRB-
cord-007261-b5fgb9wf	194	15	Fig	fig	NN
cord-007261-b5fgb9wf	194	16	.	.	.
cord-007261-b5fgb9wf	194	17	6A	6a	CD
cord-007261-b5fgb9wf	194	18	)	)	-RRB-
cord-007261-b5fgb9wf	194	19	.	.	.
cord-007261-b5fgb9wf	195	1	When	when	WRB
cord-007261-b5fgb9wf	195	2	Sec	Sec	NNP
cord-007261-b5fgb9wf	195	3	cDNA	cdna	NN
cord-007261-b5fgb9wf	195	4	was	be	VBD
cord-007261-b5fgb9wf	195	5	expressed	express	VBN
cord-007261-b5fgb9wf	195	6	in	in	IN
cord-007261-b5fgb9wf	195	7	COS	COS	NNP
cord-007261-b5fgb9wf	195	8	cells	cell	NNS
cord-007261-b5fgb9wf	195	9	,	,	,
cord-007261-b5fgb9wf	195	10	the	the	DT
cord-007261-b5fgb9wf	195	11	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	195	12	Sec	Sec	NNP
cord-007261-b5fgb9wf	195	13	protein	protein	NN
cord-007261-b5fgb9wf	195	14	also	also	RB
cord-007261-b5fgb9wf	195	15	exhibited	exhibit	VBD
cord-007261-b5fgb9wf	195	16	an	an	DT
cord-007261-b5fgb9wf	195	17	ER	ER	NNP
cord-007261-b5fgb9wf	195	18	distribution	distribution	NN
cord-007261-b5fgb9wf	195	19	(	(	-LRB-
cord-007261-b5fgb9wf	195	20	Fig	Fig	NNP
cord-007261-b5fgb9wf	195	21	.	.	.
cord-007261-b5fgb9wf	195	22	 	 	_SP
cord-007261-b5fgb9wf	195	23	6B	6b	CD
cord-007261-b5fgb9wf	195	24	)	)	-RRB-
cord-007261-b5fgb9wf	195	25	,	,	,
cord-007261-b5fgb9wf	195	26	although	although	IN
cord-007261-b5fgb9wf	195	27	it	-PRON-	PRP
cord-007261-b5fgb9wf	195	28	is	be	VBZ
cord-007261-b5fgb9wf	195	29	a	a	DT
cord-007261-b5fgb9wf	195	30	secretory	secretory	JJ
cord-007261-b5fgb9wf	195	31	protein	protein	NN
cord-007261-b5fgb9wf	195	32	.	.	.
cord-007261-b5fgb9wf	196	1	The	the	DT
cord-007261-b5fgb9wf	196	2	exit	exit	NN
cord-007261-b5fgb9wf	196	3	of	of	IN
cord-007261-b5fgb9wf	196	4	the	the	DT
cord-007261-b5fgb9wf	196	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	196	6	protein	protein	NN
cord-007261-b5fgb9wf	196	7	from	from	IN
cord-007261-b5fgb9wf	196	8	the	the	DT
cord-007261-b5fgb9wf	196	9	ER	ER	NNP
cord-007261-b5fgb9wf	196	10	was	be	VBD
cord-007261-b5fgb9wf	196	11	probably	probably	RB
cord-007261-b5fgb9wf	196	12	rate	rate	NN
cord-007261-b5fgb9wf	196	13	-	-	HYPH
cord-007261-b5fgb9wf	196	14	limiting	limit	VBG
cord-007261-b5fgb9wf	196	15	due	due	IN
cord-007261-b5fgb9wf	196	16	to	to	IN
cord-007261-b5fgb9wf	196	17	the	the	DT
cord-007261-b5fgb9wf	196	18	high	high	JJ
cord-007261-b5fgb9wf	196	19	expression	expression	NN
cord-007261-b5fgb9wf	196	20	of	of	IN
cord-007261-b5fgb9wf	196	21	the	the	DT
cord-007261-b5fgb9wf	196	22	protein	protein	NN
cord-007261-b5fgb9wf	196	23	(	(	-LRB-
cord-007261-b5fgb9wf	196	24	25	25	CD
cord-007261-b5fgb9wf	196	25	)	)	-RRB-
cord-007261-b5fgb9wf	196	26	.	.	.
cord-007261-b5fgb9wf	197	1	Similar	similar	JJ
cord-007261-b5fgb9wf	197	2	fluorescence	fluorescence	NN
cord-007261-b5fgb9wf	197	3	patterns	pattern	NNS
cord-007261-b5fgb9wf	197	4	were	be	VBD
cord-007261-b5fgb9wf	197	5	observed	observe	VBN
cord-007261-b5fgb9wf	197	6	with	with	IN
cord-007261-b5fgb9wf	197	7	the	the	DT
cord-007261-b5fgb9wf	197	8	cells	cell	NNS
cord-007261-b5fgb9wf	197	9	expressing	express	VBG
cord-007261-b5fgb9wf	197	10	Sec	Sec	NNP
cord-007261-b5fgb9wf	197	11	/	/	SYM
cord-007261-b5fgb9wf	197	12	Ml	Ml	NNP
cord-007261-b5fgb9wf	197	13	,	,	,
cord-007261-b5fgb9wf	197	14	Sec	Sec	NNP
cord-007261-b5fgb9wf	197	15	/	/	SYM
cord-007261-b5fgb9wf	197	16	Mi	Mi	NNP
cord-007261-b5fgb9wf	197	17	-	-	HYPH
cord-007261-b5fgb9wf	197	18	Pro	Pro	NNP
cord-007261-b5fgb9wf	197	19	,	,	,
cord-007261-b5fgb9wf	197	20	Sec	Sec	NNP
cord-007261-b5fgb9wf	197	21	/	/	SYM
cord-007261-b5fgb9wf	197	22	Ml	Ml	NNP
cord-007261-b5fgb9wf	197	23	-	-	HYPH
cord-007261-b5fgb9wf	197	24	TM	TM	NNP
cord-007261-b5fgb9wf	197	25	,	,	,
cord-007261-b5fgb9wf	197	26	Sec/	Sec/	NNP
cord-007261-b5fgb9wf	197	27	TM	TM	NNP
cord-007261-b5fgb9wf	197	28	-	-	HYPH
cord-007261-b5fgb9wf	197	29	H	H	NNP
cord-007261-b5fgb9wf	197	30	,	,	,
cord-007261-b5fgb9wf	197	31	Sec	Sec	NNP
cord-007261-b5fgb9wf	197	32	/	/	SYM
cord-007261-b5fgb9wf	197	33	TM	TM	NNP
cord-007261-b5fgb9wf	197	34	-	-	HYPH
cord-007261-b5fgb9wf	197	35	Q	Q	NNP
cord-007261-b5fgb9wf	197	36	,	,	,
cord-007261-b5fgb9wf	197	37	and	and	CC
cord-007261-b5fgb9wf	197	38	Sec	Sec	NNP
cord-007261-b5fgb9wf	197	39	/	/	SYM
cord-007261-b5fgb9wf	197	40	Ml	Ml	NNP
cord-007261-b5fgb9wf	197	41	-	-	HYPH
cord-007261-b5fgb9wf	197	42	GT	GT	NNP
cord-007261-b5fgb9wf	197	43	(	(	-LRB-
cord-007261-b5fgb9wf	197	44	Fig	Fig	NNP
cord-007261-b5fgb9wf	197	45	.	.	NNP
cord-007261-b5fgb9wf	197	46	6	6	CD
cord-007261-b5fgb9wf	197	47	,	,	,
cord-007261-b5fgb9wf	197	48	C	C	NNP
cord-007261-b5fgb9wf	197	49	-	-	HYPH
cord-007261-b5fgb9wf	197	50	H	H	NNP
cord-007261-b5fgb9wf	197	51	)	)	-RRB-
cord-007261-b5fgb9wf	197	52	.	.	.
cord-007261-b5fgb9wf	198	1	In	in	IN
cord-007261-b5fgb9wf	198	2	contrast	contrast	NN
cord-007261-b5fgb9wf	198	3	,	,	,
cord-007261-b5fgb9wf	198	4	the	the	DT
cord-007261-b5fgb9wf	198	5	cells	cell	NNS
cord-007261-b5fgb9wf	198	6	expressing	express	VBG
cord-007261-b5fgb9wf	198	7	Sec	Sec	NNP
cord-007261-b5fgb9wf	198	8	/	/	SYM
cord-007261-b5fgb9wf	198	9	GTd	GTd	NNS
cord-007261-b5fgb9wf	198	10	showed	show	VBD
cord-007261-b5fgb9wf	198	11	intensely	intensely	RB
cord-007261-b5fgb9wf	198	12	stained	stain	VBN
cord-007261-b5fgb9wf	198	13	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	198	14	-	-	HYPH
cord-007261-b5fgb9wf	198	15	like	like	JJ
cord-007261-b5fgb9wf	198	16	juxtanuclear	juxtanuclear	NN
cord-007261-b5fgb9wf	198	17	structures	structure	NNS
cord-007261-b5fgb9wf	198	18	(	(	-LRB-
cord-007261-b5fgb9wf	198	19	Fig	Fig	NNP
cord-007261-b5fgb9wf	198	20	.	.	.
cord-007261-b5fgb9wf	198	21	61	61	CD
cord-007261-b5fgb9wf	198	22	)	)	-RRB-
cord-007261-b5fgb9wf	198	23	,	,	,
cord-007261-b5fgb9wf	198	24	supporting	support	VBG
cord-007261-b5fgb9wf	198	25	the	the	DT
cord-007261-b5fgb9wf	198	26	above	above	JJ
cord-007261-b5fgb9wf	198	27	observations	observation	NNS
cord-007261-b5fgb9wf	198	28	that	that	IN
cord-007261-b5fgb9wf	198	29	the	the	DT
cord-007261-b5fgb9wf	198	30	sugar	sugar	NN
cord-007261-b5fgb9wf	198	31	chains	chain	NNS
cord-007261-b5fgb9wf	198	32	of	of	IN
cord-007261-b5fgb9wf	198	33	the	the	DT
cord-007261-b5fgb9wf	198	34	Sec	Sec	NNP
cord-007261-b5fgb9wf	198	35	/	/	SYM
cord-007261-b5fgb9wf	198	36	GTd	gtd	NN
cord-007261-b5fgb9wf	198	37	protein	protein	NN
cord-007261-b5fgb9wf	198	38	were	be	VBD
cord-007261-b5fgb9wf	198	39	resistant	resistant	JJ
cord-007261-b5fgb9wf	198	40	to	to	IN
cord-007261-b5fgb9wf	198	41	endo	endo	FW
cord-007261-b5fgb9wf	198	42	H	H	NNP
cord-007261-b5fgb9wf	198	43	digestion	digestion	NN
cord-007261-b5fgb9wf	198	44	and	and	CC
cord-007261-b5fgb9wf	198	45	the	the	DT
cord-007261-b5fgb9wf	198	46	expressed	express	VBN
cord-007261-b5fgb9wf	198	47	Sec	Sec	NNP
cord-007261-b5fgb9wf	198	48	/	/	SYM
cord-007261-b5fgb9wf	198	49	GTd	gtd	NN
cord-007261-b5fgb9wf	198	50	protein	protein	NN
cord-007261-b5fgb9wf	198	51	cofractionated	cofractionate	VBD
cord-007261-b5fgb9wf	198	52	with	with	IN
cord-007261-b5fgb9wf	198	53	a	a	DT
cord-007261-b5fgb9wf	198	54	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	198	55	marker	marker	NN
cord-007261-b5fgb9wf	198	56	enzyme	enzyme	NN
cord-007261-b5fgb9wf	198	57	on	on	IN
cord-007261-b5fgb9wf	198	58	sucrose	sucrose	NN
cord-007261-b5fgb9wf	198	59	density	density	NN
cord-007261-b5fgb9wf	198	60	gradient	gradient	NN
cord-007261-b5fgb9wf	198	61	centrifugation	centrifugation	NN
cord-007261-b5fgb9wf	198	62	of	of	IN
cord-007261-b5fgb9wf	198	63	the	the	DT
cord-007261-b5fgb9wf	198	64	cell	cell	NN
cord-007261-b5fgb9wf	198	65	lysates	lysate	NNS
cord-007261-b5fgb9wf	198	66	.	.	.
cord-007261-b5fgb9wf	199	1	Expression	expression	NN
cord-007261-b5fgb9wf	199	2	and	and	CC
cord-007261-b5fgb9wf	199	3	Localization	Localization	NNP
cord-007261-b5fgb9wf	199	4	of	of	IN
cord-007261-b5fgb9wf	199	5	Cathepsin	Cathepsin	NNP
cord-007261-b5fgb9wf	199	6	D	D	NNP
cord-007261-b5fgb9wf	199	7	Fusion	Fusion	NNP
cord-007261-b5fgb9wf	199	8	Proteins	Proteins	NNPS
cord-007261-b5fgb9wf	199	9	-	-	:
cord-007261-b5fgb9wf	199	10	To	to	TO
cord-007261-b5fgb9wf	199	11	determine	determine	VB
cord-007261-b5fgb9wf	199	12	whether	whether	IN
cord-007261-b5fgb9wf	199	13	or	or	CC
cord-007261-b5fgb9wf	199	14	not	not	RB
cord-007261-b5fgb9wf	199	15	P450(Ml	p450(ml	NN
cord-007261-b5fgb9wf	199	16	)	)	-RRB-
cord-007261-b5fgb9wf	199	17	is	be	VBZ
cord-007261-b5fgb9wf	199	18	recycled	recycle	VBN
cord-007261-b5fgb9wf	199	19	from	from	IN
cord-007261-b5fgb9wf	199	20	the	the	DT
cord-007261-b5fgb9wf	199	21	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	199	22	compartments	compartment	NNS
cord-007261-b5fgb9wf	199	23	,	,	,
cord-007261-b5fgb9wf	199	24	we	-PRON-	PRP
cord-007261-b5fgb9wf	199	25	used	use	VBD
cord-007261-b5fgb9wf	199	26	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	199	27	D	d	NN
cord-007261-b5fgb9wf	199	28	as	as	IN
cord-007261-b5fgb9wf	199	29	the	the	DT
cord-007261-b5fgb9wf	199	30	reporter	reporter	NN
cord-007261-b5fgb9wf	199	31	protein	protein	NN
cord-007261-b5fgb9wf	199	32	.	.	.
cord-007261-b5fgb9wf	200	1	The	the	DT
cord-007261-b5fgb9wf	200	2	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	200	3	side	side	NN
cord-007261-b5fgb9wf	200	4	chains	chain	NNS
cord-007261-b5fgb9wf	200	5	of	of	IN
cord-007261-b5fgb9wf	200	6	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	200	7	D	d	NN
cord-007261-b5fgb9wf	200	8	are	be	VBP
cord-007261-b5fgb9wf	200	9	subject	subject	JJ
cord-007261-b5fgb9wf	200	10	to	to	IN
cord-007261-b5fgb9wf	200	11	phosphorylation	phosphorylation	NN
cord-007261-b5fgb9wf	200	12	of	of	IN
cord-007261-b5fgb9wf	200	13	the	the	DT
cord-007261-b5fgb9wf	200	14	mannose	mannose	NN
cord-007261-b5fgb9wf	200	15	residues	residue	NNS
cord-007261-b5fgb9wf	200	16	at	at	IN
cord-007261-b5fgb9wf	200	17	the	the	DT
cord-007261-b5fgb9wf	200	18	6th	6th	JJ
cord-007261-b5fgb9wf	200	19	position	position	NN
cord-007261-b5fgb9wf	200	20	,	,	,
cord-007261-b5fgb9wf	200	21	this	this	DT
cord-007261-b5fgb9wf	200	22	modification	modification	NN
cord-007261-b5fgb9wf	200	23	targeting	target	VBG
cord-007261-b5fgb9wf	200	24	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	200	25	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	200	26	to	to	IN
cord-007261-b5fgb9wf	200	27	their	-PRON-	PRP$
cord-007261-b5fgb9wf	200	28	destination	destination	NN
cord-007261-b5fgb9wf	200	29	(	(	-LRB-
cord-007261-b5fgb9wf	200	30	3	3	CD
cord-007261-b5fgb9wf	200	31	,	,	,
cord-007261-b5fgb9wf	200	32	4	4	CD
cord-007261-b5fgb9wf	200	33	)	)	-RRB-
cord-007261-b5fgb9wf	200	34	.	.	.
cord-007261-b5fgb9wf	201	1	The	the	DT
cord-007261-b5fgb9wf	201	2	man	man	NN
cord-007261-b5fgb9wf	201	3	-	-	HYPH
cord-007261-b5fgb9wf	201	4	no8e-6-phosphate	no8e-6-phosphate	NN
cord-007261-b5fgb9wf	201	5	of	of	IN
cord-007261-b5fgb9wf	201	6	the	the	DT
cord-007261-b5fgb9wf	201	7	sugar	sugar	NN
cord-007261-b5fgb9wf	201	8	chains	chain	NNS
cord-007261-b5fgb9wf	201	9	is	be	VBZ
cord-007261-b5fgb9wf	201	10	formed	form	VBN
cord-007261-b5fgb9wf	201	11	through	through	IN
cord-007261-b5fgb9wf	201	12	two	two	CD
cord-007261-b5fgb9wf	201	13	steps	step	NNS
cord-007261-b5fgb9wf	201	14	,	,	,
cord-007261-b5fgb9wf	201	15	which	which	WDT
cord-007261-b5fgb9wf	201	16	occur	occur	VBP
cord-007261-b5fgb9wf	201	17	in	in	IN
cord-007261-b5fgb9wf	201	18	separate	separate	JJ
cord-007261-b5fgb9wf	201	19	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	201	20	compartments	compartment	NNS
cord-007261-b5fgb9wf	201	21	.	.	.
cord-007261-b5fgb9wf	202	1	As	as	IN
cord-007261-b5fgb9wf	202	2	the	the	DT
cord-007261-b5fgb9wf	202	3	first	first	JJ
cord-007261-b5fgb9wf	202	4	step	step	NN
cord-007261-b5fgb9wf	202	5	,	,	,
cord-007261-b5fgb9wf	202	6	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	202	7	-	-	HYPH
cord-007261-b5fgb9wf	202	8	P	P	NNP
cord-007261-b5fgb9wf	202	9	is	be	VBZ
cord-007261-b5fgb9wf	202	10	linked	link	VBN
cord-007261-b5fgb9wf	202	11	to	to	IN
cord-007261-b5fgb9wf	202	12	mannose	mannose	NN
cord-007261-b5fgb9wf	202	13	to	to	TO
cord-007261-b5fgb9wf	202	14	give	give	VB
cord-007261-b5fgb9wf	202	15	a	a	DT
cord-007261-b5fgb9wf	202	16	phosphodiester	phosphodiester	NN
cord-007261-b5fgb9wf	202	17	.	.	.
cord-007261-b5fgb9wf	203	1	The	the	DT
cord-007261-b5fgb9wf	203	2	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	203	3	residue	residue	NN
cord-007261-b5fgb9wf	203	4	is	be	VBZ
cord-007261-b5fgb9wf	203	5	subsequently	subsequently	RB
cord-007261-b5fgb9wf	203	6	removed	remove	VBN
cord-007261-b5fgb9wf	203	7	by	by	IN
cord-007261-b5fgb9wf	203	8	the	the	DT
cord-007261-b5fgb9wf	203	9	second	second	JJ
cord-007261-b5fgb9wf	203	10	enzyme	enzyme	NN
cord-007261-b5fgb9wf	203	11	to	to	TO
cord-007261-b5fgb9wf	203	12	form	form	VB
cord-007261-b5fgb9wf	203	13	a	a	DT
cord-007261-b5fgb9wf	203	14	mannose-6-phosphate	mannose-6-phosphate	NNP
cord-007261-b5fgb9wf	203	15	residue	residue	NN
cord-007261-b5fgb9wf	203	16	,	,	,
cord-007261-b5fgb9wf	203	17	whose	whose	WP$
cord-007261-b5fgb9wf	203	18	phosphomonoester	phosphomonoest	JJR
cord-007261-b5fgb9wf	203	19	bond	bond	NN
cord-007261-b5fgb9wf	203	20	is	be	VBZ
cord-007261-b5fgb9wf	203	21	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	203	22	to	to	IN
cord-007261-b5fgb9wf	203	23	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	203	24	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	203	25	.	.	.
cord-007261-b5fgb9wf	204	1	The	the	DT
cord-007261-b5fgb9wf	204	2	first	first	JJ
cord-007261-b5fgb9wf	204	3	enzyme	enzyme	NN
cord-007261-b5fgb9wf	204	4	is	be	VBZ
cord-007261-b5fgb9wf	204	5	located	locate	VBN
cord-007261-b5fgb9wf	204	6	in	in	IN
cord-007261-b5fgb9wf	204	7	the	the	DT
cord-007261-b5fgb9wf	204	8	cis	cis	NNP
cord-007261-b5fgb9wf	204	9	-	-	HYPH
cord-007261-b5fgb9wf	204	10	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	204	11	compartment	compartment	NN
cord-007261-b5fgb9wf	204	12	,	,	,
cord-007261-b5fgb9wf	204	13	whereas	whereas	IN
cord-007261-b5fgb9wf	204	14	the	the	DT
cord-007261-b5fgb9wf	204	15	second	second	JJ
cord-007261-b5fgb9wf	204	16	step	step	NN
cord-007261-b5fgb9wf	204	17	occurs	occur	VBZ
cord-007261-b5fgb9wf	204	18	in	in	IN
cord-007261-b5fgb9wf	204	19	the	the	DT
cord-007261-b5fgb9wf	204	20	medial	medial	JJ
cord-007261-b5fgb9wf	204	21	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	204	22	compartment	compartment	NN
cord-007261-b5fgb9wf	204	23	(	(	-LRB-
cord-007261-b5fgb9wf	204	24	26	26	CD
cord-007261-b5fgb9wf	204	25	)	)	-RRB-
cord-007261-b5fgb9wf	204	26	(	(	-LRB-
cord-007261-b5fgb9wf	204	27	27	27	CD
cord-007261-b5fgb9wf	204	28	)	)	-RRB-
cord-007261-b5fgb9wf	204	29	(	(	-LRB-
cord-007261-b5fgb9wf	204	30	28	28	CD
cord-007261-b5fgb9wf	204	31	)	)	-RRB-
cord-007261-b5fgb9wf	204	32	(	(	-LRB-
cord-007261-b5fgb9wf	204	33	29	29	CD
cord-007261-b5fgb9wf	204	34	)	)	-RRB-
cord-007261-b5fgb9wf	204	35	.	.	.
cord-007261-b5fgb9wf	205	1	Investigations	investigation	NNS
cord-007261-b5fgb9wf	205	2	on	on	IN
cord-007261-b5fgb9wf	205	3	the	the	DT
cord-007261-b5fgb9wf	205	4	formation	formation	NN
cord-007261-b5fgb9wf	205	5	of	of	IN
cord-007261-b5fgb9wf	205	6	the	the	DT
cord-007261-b5fgb9wf	205	7	mannose-6-phosphate	mannose-6-phosphate	NNP
cord-007261-b5fgb9wf	205	8	residue	residue	NN
cord-007261-b5fgb9wf	205	9	of	of	IN
cord-007261-b5fgb9wf	205	10	the	the	DT
cord-007261-b5fgb9wf	205	11	sugar	sugar	NN
cord-007261-b5fgb9wf	205	12	chains	chain	NNS
cord-007261-b5fgb9wf	205	13	of	of	IN
cord-007261-b5fgb9wf	205	14	the	the	DT
cord-007261-b5fgb9wf	205	15	fusion	fusion	NN
cord-007261-b5fgb9wf	205	16	protein	protein	NN
cord-007261-b5fgb9wf	205	17	,	,	,
cord-007261-b5fgb9wf	205	18	P450(Ml)-cathepsin	P450(Ml)-cathepsin	NNP
cord-007261-b5fgb9wf	205	19	D	D	NNP
cord-007261-b5fgb9wf	205	20	,	,	,
cord-007261-b5fgb9wf	205	21	will	will	MD
cord-007261-b5fgb9wf	205	22	show	show	VB
cord-007261-b5fgb9wf	205	23	us	-PRON-	PRP
cord-007261-b5fgb9wf	205	24	whether	whether	IN
cord-007261-b5fgb9wf	205	25	the	the	DT
cord-007261-b5fgb9wf	205	26	fusion	fusion	NN
cord-007261-b5fgb9wf	205	27	protein	protein	NN
cord-007261-b5fgb9wf	205	28	goes	go	VBZ
cord-007261-b5fgb9wf	205	29	to	to	IN
cord-007261-b5fgb9wf	205	30	a	a	DT
cord-007261-b5fgb9wf	205	31	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	205	32	compartment	compartment	NN
cord-007261-b5fgb9wf	205	33	or	or	CC
cord-007261-b5fgb9wf	205	34	not	not	RB
cord-007261-b5fgb9wf	205	35	.	.	.
cord-007261-b5fgb9wf	206	1	The	the	DT
cord-007261-b5fgb9wf	206	2	fusion	fusion	NN
cord-007261-b5fgb9wf	206	3	protein	protein	NN
cord-007261-b5fgb9wf	206	4	(	(	-LRB-
cord-007261-b5fgb9wf	206	5	CathD	CathD	NNP
cord-007261-b5fgb9wf	206	6	/	/	SYM
cord-007261-b5fgb9wf	206	7	Ml	Ml	NNP
cord-007261-b5fgb9wf	206	8	in	in	IN
cord-007261-b5fgb9wf	206	9	Fig	Fig	NNP
cord-007261-b5fgb9wf	206	10	.	.	.
cord-007261-b5fgb9wf	207	1	1	1	LS
cord-007261-b5fgb9wf	207	2	)	)	-RRB-
cord-007261-b5fgb9wf	207	3	was	be	VBD
cord-007261-b5fgb9wf	207	4	expressed	express	VBN
cord-007261-b5fgb9wf	207	5	in	in	IN
cord-007261-b5fgb9wf	207	6	COS	COS	NNP
cord-007261-b5fgb9wf	207	7	cells	cell	NNS
cord-007261-b5fgb9wf	207	8	.	.	.
cord-007261-b5fgb9wf	208	1	To	to	TO
cord-007261-b5fgb9wf	208	2	confirm	confirm	VB
cord-007261-b5fgb9wf	208	3	the	the	DT
cord-007261-b5fgb9wf	208	4	expression	expression	NN
cord-007261-b5fgb9wf	208	5	of	of	IN
cord-007261-b5fgb9wf	208	6	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	208	7	D	d	NN
cord-007261-b5fgb9wf	208	8	,	,	,
cord-007261-b5fgb9wf	208	9	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	208	10	)	)	-RRB-
cord-007261-b5fgb9wf	208	11	and	and	CC
cord-007261-b5fgb9wf	208	12	the	the	DT
cord-007261-b5fgb9wf	208	13	CathD	CathD	NNP
cord-007261-b5fgb9wf	208	14	/	/	SYM
cord-007261-b5fgb9wf	208	15	Ml	Ml	NNP
cord-007261-b5fgb9wf	208	16	protein	protein	NN
cord-007261-b5fgb9wf	208	17	,	,	,
cord-007261-b5fgb9wf	208	18	the	the	DT
cord-007261-b5fgb9wf	208	19	cells	cell	NNS
cord-007261-b5fgb9wf	208	20	were	be	VBD
cord-007261-b5fgb9wf	208	21	labeled	label	VBN
cord-007261-b5fgb9wf	208	22	with	with	IN
cord-007261-b5fgb9wf	208	23	[	[	-LRB-
cord-007261-b5fgb9wf	208	24	35	35	CD
cord-007261-b5fgb9wf	208	25	S]methionine	S]methionine	NNP
cord-007261-b5fgb9wf	208	26	for	for	IN
cord-007261-b5fgb9wf	208	27	3	3	CD
cord-007261-b5fgb9wf	208	28	h	h	NN
cord-007261-b5fgb9wf	208	29	,	,	,
cord-007261-b5fgb9wf	208	30	and	and	CC
cord-007261-b5fgb9wf	208	31	then	then	RB
cord-007261-b5fgb9wf	208	32	the	the	DT
cord-007261-b5fgb9wf	208	33	labeled	label	VBN
cord-007261-b5fgb9wf	208	34	proteins	protein	NNS
cord-007261-b5fgb9wf	208	35	in	in	IN
cord-007261-b5fgb9wf	208	36	the	the	DT
cord-007261-b5fgb9wf	208	37	cells	cell	NNS
cord-007261-b5fgb9wf	208	38	and	and	CC
cord-007261-b5fgb9wf	208	39	the	the	DT
cord-007261-b5fgb9wf	208	40	medium	medium	NN
cord-007261-b5fgb9wf	208	41	were	be	VBD
cord-007261-b5fgb9wf	208	42	analyzed	analyze	VBN
cord-007261-b5fgb9wf	208	43	by	by	IN
cord-007261-b5fgb9wf	208	44	immunoprecipitation	immunoprecipitation	NN
cord-007261-b5fgb9wf	208	45	with	with	IN
cord-007261-b5fgb9wf	208	46	appropriate	appropriate	JJ
cord-007261-b5fgb9wf	208	47	antibodies	antibody	NNS
cord-007261-b5fgb9wf	208	48	and	and	CC
cord-007261-b5fgb9wf	208	49	SDS	sds	NN
cord-007261-b5fgb9wf	208	50	-	-	HYPH
cord-007261-b5fgb9wf	208	51	PAGE	PAGE	NNP
cord-007261-b5fgb9wf	208	52	of	of	IN
cord-007261-b5fgb9wf	208	53	the	the	DT
cord-007261-b5fgb9wf	208	54	immunoprecipitates	immunoprecipitate	NNS
cord-007261-b5fgb9wf	208	55	(	(	-LRB-
cord-007261-b5fgb9wf	208	56	Fig	Fig	NNP
cord-007261-b5fgb9wf	208	57	.	.	NNP
cord-007261-b5fgb9wf	208	58	7	7	CD
cord-007261-b5fgb9wf	208	59	)	)	-RRB-
cord-007261-b5fgb9wf	208	60	.	.	.
cord-007261-b5fgb9wf	209	1	Since	since	IN
cord-007261-b5fgb9wf	209	2	no	no	DT
cord-007261-b5fgb9wf	209	3	detectable	detectable	JJ
cord-007261-b5fgb9wf	209	4	band	band	NN
cord-007261-b5fgb9wf	209	5	of	of	IN
cord-007261-b5fgb9wf	209	6	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	209	7	D	d	NN
cord-007261-b5fgb9wf	209	8	or	or	CC
cord-007261-b5fgb9wf	209	9	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	209	10	)	)	-RRB-
cord-007261-b5fgb9wf	209	11	was	be	VBD
cord-007261-b5fgb9wf	209	12	observed	observe	VBN
cord-007261-b5fgb9wf	209	13	with	with	IN
cord-007261-b5fgb9wf	209	14	the	the	DT
cord-007261-b5fgb9wf	209	15	mock	mock	NN
cord-007261-b5fgb9wf	209	16	-	-	HYPH
cord-007261-b5fgb9wf	209	17	transfected	transfecte	VBN
cord-007261-b5fgb9wf	209	18	cells	cell	NNS
cord-007261-b5fgb9wf	209	19	,	,	,
cord-007261-b5fgb9wf	209	20	the	the	DT
cord-007261-b5fgb9wf	209	21	antibodies	antibody	NNS
cord-007261-b5fgb9wf	209	22	against	against	IN
cord-007261-b5fgb9wf	209	23	rat	rat	NN
cord-007261-b5fgb9wf	209	24	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	209	25	D	d	NN
cord-007261-b5fgb9wf	209	26	and	and	CC
cord-007261-b5fgb9wf	209	27	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	209	28	)	)	-RRB-
cord-007261-b5fgb9wf	209	29	used	use	VBN
cord-007261-b5fgb9wf	209	30	in	in	IN
cord-007261-b5fgb9wf	209	31	this	this	DT
cord-007261-b5fgb9wf	209	32	experiment	experiment	NN
cord-007261-b5fgb9wf	209	33	did	do	VBD
cord-007261-b5fgb9wf	209	34	not	not	RB
cord-007261-b5fgb9wf	209	35	cross	cross	VB
cord-007261-b5fgb9wf	209	36	-	-	JJ
cord-007261-b5fgb9wf	209	37	react	react	VB
cord-007261-b5fgb9wf	209	38	with	with	IN
cord-007261-b5fgb9wf	209	39	the	the	DT
cord-007261-b5fgb9wf	209	40	endogenous	endogenous	JJ
cord-007261-b5fgb9wf	209	41	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	209	42	or	or	CC
cord-007261-b5fgb9wf	209	43	P450	P450	NNPS
cord-007261-b5fgb9wf	209	44	of	of	IN
cord-007261-b5fgb9wf	209	45	the	the	DT
cord-007261-b5fgb9wf	209	46	COS	COS	NNP
cord-007261-b5fgb9wf	209	47	cells	cell	NNS
cord-007261-b5fgb9wf	210	1	(	(	-LRB-
cord-007261-b5fgb9wf	210	2	Fig	Fig	NNP
cord-007261-b5fgb9wf	210	3	.	.	NNP
cord-007261-b5fgb9wf	210	4	7	7	CD
cord-007261-b5fgb9wf	210	5	,	,	,
cord-007261-b5fgb9wf	210	6	lanes	lane	NNS
cord-007261-b5fgb9wf	210	7	1	1	CD
cord-007261-b5fgb9wf	210	8	-	-	SYM
cord-007261-b5fgb9wf	210	9	4	4	CD
cord-007261-b5fgb9wf	210	10	)	)	-RRB-
cord-007261-b5fgb9wf	210	11	.	.	.
cord-007261-b5fgb9wf	211	1	Two	two	CD
cord-007261-b5fgb9wf	211	2	bands	band	NNS
cord-007261-b5fgb9wf	211	3	with	with	IN
cord-007261-b5fgb9wf	211	4	close	close	JJ
cord-007261-b5fgb9wf	211	5	mobilities	mobility	NNS
cord-007261-b5fgb9wf	211	6	were	be	VBD
cord-007261-b5fgb9wf	211	7	observed	observe	VBN
cord-007261-b5fgb9wf	211	8	with	with	IN
cord-007261-b5fgb9wf	211	9	the	the	DT
cord-007261-b5fgb9wf	211	10	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	211	11	products	product	NNS
cord-007261-b5fgb9wf	211	12	of	of	IN
cord-007261-b5fgb9wf	211	13	the	the	DT
cord-007261-b5fgb9wf	211	14	transfected	transfecte	VBN
cord-007261-b5fgb9wf	211	15	CathD	CathD	NNP
cord-007261-b5fgb9wf	211	16	cDNA	cdna	NN
cord-007261-b5fgb9wf	211	17	(	(	-LRB-
cord-007261-b5fgb9wf	211	18	Fig	Fig	NNP
cord-007261-b5fgb9wf	211	19	.	.	NNP
cord-007261-b5fgb9wf	211	20	7	7	CD
cord-007261-b5fgb9wf	211	21	,	,	,
cord-007261-b5fgb9wf	211	22	lane	lane	NN
cord-007261-b5fgb9wf	211	23	5	5	CD
cord-007261-b5fgb9wf	211	24	)	)	-RRB-
cord-007261-b5fgb9wf	211	25	.	.	.
cord-007261-b5fgb9wf	212	1	A	a	DT
cord-007261-b5fgb9wf	212	2	band	band	NN
cord-007261-b5fgb9wf	212	3	with	with	IN
cord-007261-b5fgb9wf	212	4	the	the	DT
cord-007261-b5fgb9wf	212	5	same	same	JJ
cord-007261-b5fgb9wf	212	6	mobility	mobility	NN
cord-007261-b5fgb9wf	212	7	as	as	IN
cord-007261-b5fgb9wf	212	8	the	the	DT
cord-007261-b5fgb9wf	212	9	lower	low	JJR
cord-007261-b5fgb9wf	212	10	band	band	NN
cord-007261-b5fgb9wf	212	11	was	be	VBD
cord-007261-b5fgb9wf	212	12	also	also	RB
cord-007261-b5fgb9wf	212	13	detected	detect	VBN
cord-007261-b5fgb9wf	212	14	for	for	IN
cord-007261-b5fgb9wf	212	15	the	the	DT
cord-007261-b5fgb9wf	212	16	medium	medium	NN
cord-007261-b5fgb9wf	212	17	(	(	-LRB-
cord-007261-b5fgb9wf	212	18	Fig	Fig	NNP
cord-007261-b5fgb9wf	212	19	.	.	NNP
cord-007261-b5fgb9wf	212	20	7	7	CD
cord-007261-b5fgb9wf	212	21	,	,	,
cord-007261-b5fgb9wf	212	22	lane	lane	NN
cord-007261-b5fgb9wf	212	23	6	6	CD
cord-007261-b5fgb9wf	212	24	)	)	-RRB-
cord-007261-b5fgb9wf	212	25	.	.	.
cord-007261-b5fgb9wf	213	1	The	the	DT
cord-007261-b5fgb9wf	213	2	lower	low	JJR
cord-007261-b5fgb9wf	213	3	band	band	NN
cord-007261-b5fgb9wf	213	4	was	be	VBD
cord-007261-b5fgb9wf	213	5	probably	probably	RB
cord-007261-b5fgb9wf	213	6	the	the	DT
cord-007261-b5fgb9wf	213	7	pro	pro	JJ
cord-007261-b5fgb9wf	213	8	-	-	JJ
cord-007261-b5fgb9wf	213	9	form	form	NN
cord-007261-b5fgb9wf	213	10	of	of	IN
cord-007261-b5fgb9wf	213	11	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	213	12	D	d	NN
cord-007261-b5fgb9wf	213	13	,	,	,
cord-007261-b5fgb9wf	213	14	but	but	CC
cord-007261-b5fgb9wf	213	15	the	the	DT
cord-007261-b5fgb9wf	213	16	nature	nature	NN
cord-007261-b5fgb9wf	213	17	of	of	IN
cord-007261-b5fgb9wf	213	18	the	the	DT
cord-007261-b5fgb9wf	213	19	upper	upper	JJ
cord-007261-b5fgb9wf	213	20	band	band	NN
cord-007261-b5fgb9wf	213	21	was	be	VBD
cord-007261-b5fgb9wf	213	22	not	not	RB
cord-007261-b5fgb9wf	213	23	clear	clear	JJ
cord-007261-b5fgb9wf	213	24	.	.	.
cord-007261-b5fgb9wf	214	1	The	the	DT
cord-007261-b5fgb9wf	214	2	secretion	secretion	NN
cord-007261-b5fgb9wf	214	3	of	of	IN
cord-007261-b5fgb9wf	214	4	the	the	DT
cord-007261-b5fgb9wf	214	5	pro	pro	JJ
cord-007261-b5fgb9wf	214	6	form	form	NN
cord-007261-b5fgb9wf	214	7	of	of	IN
cord-007261-b5fgb9wf	214	8	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	214	9	D	d	NN
cord-007261-b5fgb9wf	214	10	was	be	VBD
cord-007261-b5fgb9wf	214	11	possibly	possibly	RB
cord-007261-b5fgb9wf	214	12	caused	cause	VBN
cord-007261-b5fgb9wf	214	13	by	by	IN
cord-007261-b5fgb9wf	214	14	the	the	DT
cord-007261-b5fgb9wf	214	15	overexpression	overexpression	NN
cord-007261-b5fgb9wf	214	16	of	of	IN
cord-007261-b5fgb9wf	214	17	the	the	DT
cord-007261-b5fgb9wf	214	18	enzyme	enzyme	NN
cord-007261-b5fgb9wf	214	19	in	in	IN
cord-007261-b5fgb9wf	214	20	the	the	DT
cord-007261-b5fgb9wf	214	21	COS	COS	NNP
cord-007261-b5fgb9wf	214	22	cells	cell	NNS
cord-007261-b5fgb9wf	214	23	.	.	.
cord-007261-b5fgb9wf	215	1	The	the	DT
cord-007261-b5fgb9wf	215	2	Ml	Ml	NNP
cord-007261-b5fgb9wf	215	3	(	(	-LRB-
cord-007261-b5fgb9wf	215	4	Fig	Fig	NNP
cord-007261-b5fgb9wf	215	5	.	.	NNP
cord-007261-b5fgb9wf	215	6	7	7	CD
cord-007261-b5fgb9wf	215	7	,	,	,
cord-007261-b5fgb9wf	215	8	lanes	lane	NNS
cord-007261-b5fgb9wf	215	9	7	7	CD
cord-007261-b5fgb9wf	215	10	and	and	CC
cord-007261-b5fgb9wf	215	11	8)	8)	NN
cord-007261-b5fgb9wf	215	12	and	and	CC
cord-007261-b5fgb9wf	215	13	CathD/	cathd/	NN
cord-007261-b5fgb9wf	216	1	Ml	Ml	NNP
cord-007261-b5fgb9wf	216	2	(	(	-LRB-
cord-007261-b5fgb9wf	216	3	Fig	fig	NN
cord-007261-b5fgb9wf	216	4	.	.	.
cord-007261-b5fgb9wf	217	1	7	7	CD
cord-007261-b5fgb9wf	217	2	,	,	,
cord-007261-b5fgb9wf	217	3	lanes	lane	NNS
cord-007261-b5fgb9wf	217	4	9	9	CD
cord-007261-b5fgb9wf	217	5	-	-	SYM
cord-007261-b5fgb9wf	217	6	12	12	CD
cord-007261-b5fgb9wf	217	7	)	)	-RRB-
cord-007261-b5fgb9wf	217	8	products	product	NNS
cord-007261-b5fgb9wf	217	9	accumulated	accumulate	VBN
cord-007261-b5fgb9wf	217	10	in	in	IN
cord-007261-b5fgb9wf	217	11	the	the	DT
cord-007261-b5fgb9wf	217	12	cells	cell	NNS
cord-007261-b5fgb9wf	217	13	,	,	,
cord-007261-b5fgb9wf	217	14	but	but	CC
cord-007261-b5fgb9wf	217	15	were	be	VBD
cord-007261-b5fgb9wf	217	16	not	not	RB
cord-007261-b5fgb9wf	217	17	detected	detect	VBN
cord-007261-b5fgb9wf	217	18	in	in	IN
cord-007261-b5fgb9wf	217	19	the	the	DT
cord-007261-b5fgb9wf	217	20	medium	medium	NN
cord-007261-b5fgb9wf	217	21	.	.	.
cord-007261-b5fgb9wf	218	1	When	when	WRB
cord-007261-b5fgb9wf	218	2	examined	examine	VBN
cord-007261-b5fgb9wf	218	3	morphologically	morphologically	RB
cord-007261-b5fgb9wf	218	4	by	by	IN
cord-007261-b5fgb9wf	218	5	means	mean	NNS
cord-007261-b5fgb9wf	218	6	of	of	IN
cord-007261-b5fgb9wf	218	7	immunofluorescence	immunofluorescence	NN
cord-007261-b5fgb9wf	218	8	microscopy	microscopy	NN
cord-007261-b5fgb9wf	218	9	with	with	IN
cord-007261-b5fgb9wf	218	10	the	the	DT
cord-007261-b5fgb9wf	218	11	antibodies	antibody	NNS
cord-007261-b5fgb9wf	218	12	to	to	TO
cord-007261-b5fgb9wf	218	13	cathepsin	cathepsin	VB
cord-007261-b5fgb9wf	218	14	D	D	NNP
cord-007261-b5fgb9wf	218	15	and	and	CC
cord-007261-b5fgb9wf	218	16	p450(Ml	p450(Ml	NNP
cord-007261-b5fgb9wf	218	17	)	)	-RRB-
cord-007261-b5fgb9wf	218	18	,	,	,
cord-007261-b5fgb9wf	218	19	the	the	DT
cord-007261-b5fgb9wf	218	20	cells	cell	NNS
cord-007261-b5fgb9wf	218	21	expressing	express	VBG
cord-007261-b5fgb9wf	218	22	the	the	DT
cord-007261-b5fgb9wf	218	23	original	original	JJ
cord-007261-b5fgb9wf	218	24	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	218	25	D	d	NN
cord-007261-b5fgb9wf	218	26	showed	show	VBD
cord-007261-b5fgb9wf	218	27	intensely	intensely	RB
cord-007261-b5fgb9wf	218	28	stained	stain	VBN
cord-007261-b5fgb9wf	218	29	lysosomes	lysosome	NNS
cord-007261-b5fgb9wf	218	30	.	.	.
cord-007261-b5fgb9wf	219	1	The	the	DT
cord-007261-b5fgb9wf	219	2	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	219	3	apparatus	apparatus	NN
cord-007261-b5fgb9wf	219	4	near	near	IN
cord-007261-b5fgb9wf	219	5	the	the	DT
cord-007261-b5fgb9wf	219	6	nucleus	nucleus	NN
cord-007261-b5fgb9wf	219	7	was	be	VBD
cord-007261-b5fgb9wf	219	8	also	also	RB
cord-007261-b5fgb9wf	219	9	stained	stain	VBN
cord-007261-b5fgb9wf	219	10	(	(	-LRB-
cord-007261-b5fgb9wf	219	11	Fig	fig	NN
cord-007261-b5fgb9wf	219	12	.	.	.
cord-007261-b5fgb9wf	219	13	8A	8a	NN
cord-007261-b5fgb9wf	219	14	)	)	-RRB-
cord-007261-b5fgb9wf	219	15	.	.	.
cord-007261-b5fgb9wf	220	1	On	on	IN
cord-007261-b5fgb9wf	220	2	the	the	DT
cord-007261-b5fgb9wf	220	3	other	other	JJ
cord-007261-b5fgb9wf	220	4	hand	hand	NN
cord-007261-b5fgb9wf	220	5	,	,	,
cord-007261-b5fgb9wf	220	6	the	the	DT
cord-007261-b5fgb9wf	220	7	cells	cell	NNS
cord-007261-b5fgb9wf	220	8	expressing	express	VBG
cord-007261-b5fgb9wf	220	9	cathD	cathD	NNP
cord-007261-b5fgb9wf	220	10	/	/	SYM
cord-007261-b5fgb9wf	220	11	Ml	Ml	NNP
cord-007261-b5fgb9wf	220	12	showed	show	VBD
cord-007261-b5fgb9wf	220	13	a	a	DT
cord-007261-b5fgb9wf	220	14	typical	typical	JJ
cord-007261-b5fgb9wf	220	15	ER	ER	NNP
cord-007261-b5fgb9wf	220	16	pattern	pattern	NN
cord-007261-b5fgb9wf	220	17	of	of	IN
cord-007261-b5fgb9wf	220	18	staining	stain	VBG
cord-007261-b5fgb9wf	220	19	with	with	IN
cord-007261-b5fgb9wf	220	20	no	no	DT
cord-007261-b5fgb9wf	220	21	detectable	detectable	JJ
cord-007261-b5fgb9wf	220	22	fluorescence	fluorescence	NN
cord-007261-b5fgb9wf	220	23	in	in	IN
cord-007261-b5fgb9wf	220	24	the	the	DT
cord-007261-b5fgb9wf	220	25	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	220	26	region	region	NN
cord-007261-b5fgb9wf	220	27	(	(	-LRB-
cord-007261-b5fgb9wf	220	28	Fig	Fig	NNP
cord-007261-b5fgb9wf	220	29	.	.	.
cord-007261-b5fgb9wf	220	30	8B	8b	NN
cord-007261-b5fgb9wf	220	31	)	)	-RRB-
cord-007261-b5fgb9wf	220	32	.	.	.
cord-007261-b5fgb9wf	221	1	This	this	DT
cord-007261-b5fgb9wf	221	2	confirms	confirm	VBZ
cord-007261-b5fgb9wf	221	3	the	the	DT
cord-007261-b5fgb9wf	221	4	ER	ER	NNP
cord-007261-b5fgb9wf	221	5	localization	localization	NN
cord-007261-b5fgb9wf	221	6	of	of	IN
cord-007261-b5fgb9wf	221	7	the	the	DT
cord-007261-b5fgb9wf	221	8	fusion	fusion	NN
cord-007261-b5fgb9wf	221	9	protein	protein	NN
cord-007261-b5fgb9wf	221	10	.	.	.
cord-007261-b5fgb9wf	222	1	To	to	TO
cord-007261-b5fgb9wf	222	2	determine	determine	VB
cord-007261-b5fgb9wf	222	3	whether	whether	IN
cord-007261-b5fgb9wf	222	4	or	or	CC
cord-007261-b5fgb9wf	222	5	not	not	RB
cord-007261-b5fgb9wf	222	6	the	the	DT
cord-007261-b5fgb9wf	222	7	CathD	CathD	NNP
cord-007261-b5fgb9wf	222	8	/	/	SYM
cord-007261-b5fgb9wf	222	9	Ml	Ml	NNP
cord-007261-b5fgb9wf	222	10	fusion	fusion	NN
cord-007261-b5fgb9wf	222	11	protein	protein	NN
cord-007261-b5fgb9wf	222	12	was	be	VBD
cord-007261-b5fgb9wf	222	13	recycled	recycle	VBN
cord-007261-b5fgb9wf	222	14	from	from	IN
cord-007261-b5fgb9wf	222	15	a	a	DT
cord-007261-b5fgb9wf	222	16	post	post	JJ
cord-007261-b5fgb9wf	222	17	-	-	JJ
cord-007261-b5fgb9wf	222	18	ER	er	JJ
cord-007261-b5fgb9wf	222	19	compartment	compartment	NN
cord-007261-b5fgb9wf	222	20	,	,	,
cord-007261-b5fgb9wf	222	21	cells	cell	NNS
cord-007261-b5fgb9wf	222	22	transfected	transfecte	VBN
cord-007261-b5fgb9wf	222	23	with	with	IN
cord-007261-b5fgb9wf	222	24	CathD	CathD	NNP
cord-007261-b5fgb9wf	222	25	,	,	,
cord-007261-b5fgb9wf	222	26	Ml	Ml	NNP
cord-007261-b5fgb9wf	222	27	or	or	CC
cord-007261-b5fgb9wf	222	28	CathD	CathD	NNP
cord-007261-b5fgb9wf	223	1	/Ml	/Ml	NNP
cord-007261-b5fgb9wf	223	2	were	be	VBD
cord-007261-b5fgb9wf	223	3	labeled	label	VBN
cord-007261-b5fgb9wf	223	4	with	with	IN
cord-007261-b5fgb9wf	223	5	S2	S2	NNP
cord-007261-b5fgb9wf	223	6	PO	PO	NNP
cord-007261-b5fgb9wf	223	7	4	4	CD
cord-007261-b5fgb9wf	223	8	for	for	IN
cord-007261-b5fgb9wf	223	9	2	2	CD
cord-007261-b5fgb9wf	223	10	h.	h.	NN
cord-007261-b5fgb9wf	224	1	The	the	DT
cord-007261-b5fgb9wf	224	2	expressed	express	VBN
cord-007261-b5fgb9wf	224	3	proteins	protein	NNS
cord-007261-b5fgb9wf	224	4	were	be	VBD
cord-007261-b5fgb9wf	224	5	immunoprecipitated	immunoprecipitate	VBN
cord-007261-b5fgb9wf	224	6	with	with	IN
cord-007261-b5fgb9wf	224	7	anti	anti	JJ
cord-007261-b5fgb9wf	224	8	-	-	NNP
cord-007261-b5fgb9wf	224	9	Mi	Mi	NNP
cord-007261-b5fgb9wf	224	10	for	for	IN
cord-007261-b5fgb9wf	224	11	Ml	Ml	NNP
cord-007261-b5fgb9wf	224	12	,	,	,
cord-007261-b5fgb9wf	224	13	and	and	CC
cord-007261-b5fgb9wf	224	14	with	with	IN
cord-007261-b5fgb9wf	224	15	anti	anti	JJ
cord-007261-b5fgb9wf	224	16	-	-	JJ
cord-007261-b5fgb9wf	224	17	CathD	CathD	NNP
cord-007261-b5fgb9wf	224	18	for	for	IN
cord-007261-b5fgb9wf	224	19	the	the	DT
cord-007261-b5fgb9wf	224	20	CathD	CathD	NNP
cord-007261-b5fgb9wf	224	21	and	and	CC
cord-007261-b5fgb9wf	224	22	CathD	CathD	NNP
cord-007261-b5fgb9wf	224	23	/	/	SYM
cord-007261-b5fgb9wf	224	24	Ml	Ml	NNP
cord-007261-b5fgb9wf	224	25	products	product	NNS
cord-007261-b5fgb9wf	224	26	.	.	.
cord-007261-b5fgb9wf	225	1	The	the	DT
cord-007261-b5fgb9wf	225	2	CathD	CathD	NNP
cord-007261-b5fgb9wf	225	3	,	,	,
cord-007261-b5fgb9wf	225	4	Ml	Ml	NNP
cord-007261-b5fgb9wf	225	5	,	,	,
cord-007261-b5fgb9wf	225	6	and	and	CC
cord-007261-b5fgb9wf	225	7	CathD/	cathd/	DT
cord-007261-b5fgb9wf	226	1	Ml	ml	NN
cord-007261-b5fgb9wf	226	2	products	product	NNS
cord-007261-b5fgb9wf	226	3	were	be	VBD
cord-007261-b5fgb9wf	226	4	all	all	DT
cord-007261-b5fgb9wf	226	5	phosphorylated	phosphorylated	JJ
cord-007261-b5fgb9wf	226	6	(	(	-LRB-
cord-007261-b5fgb9wf	226	7	Fig	Fig	NNP
cord-007261-b5fgb9wf	226	8	.	.	NNP
cord-007261-b5fgb9wf	226	9	9	9	CD
cord-007261-b5fgb9wf	226	10	,	,	,
cord-007261-b5fgb9wf	226	11	lanes	lane	NNS
cord-007261-b5fgb9wf	226	12	 	 	_SP
cord-007261-b5fgb9wf	226	13	1	1	CD
cord-007261-b5fgb9wf	226	14	,	,	,
cord-007261-b5fgb9wf	226	15	3	3	CD
cord-007261-b5fgb9wf	226	16	,	,	,
cord-007261-b5fgb9wf	226	17	and	and	CC
cord-007261-b5fgb9wf	226	18	5	5	CD
cord-007261-b5fgb9wf	226	19	)	)	-RRB-
cord-007261-b5fgb9wf	226	20	.	.	.
cord-007261-b5fgb9wf	227	1	To	to	TO
cord-007261-b5fgb9wf	227	2	define	define	VB
cord-007261-b5fgb9wf	227	3	the	the	DT
cord-007261-b5fgb9wf	227	4	structures	structure	NNS
cord-007261-b5fgb9wf	227	5	of	of	IN
cord-007261-b5fgb9wf	227	6	the	the	DT
cord-007261-b5fgb9wf	227	7	phosphorylated	phosphorylated	JJ
cord-007261-b5fgb9wf	227	8	products	product	NNS
cord-007261-b5fgb9wf	227	9	,	,	,
cord-007261-b5fgb9wf	227	10	they	-PRON-	PRP
cord-007261-b5fgb9wf	227	11	were	be	VBD
cord-007261-b5fgb9wf	227	12	treated	treat	VBN
cord-007261-b5fgb9wf	227	13	with	with	IN
cord-007261-b5fgb9wf	227	14	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	227	15	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	227	16	.	.	.
cord-007261-b5fgb9wf	228	1	Mannose-6-P	Mannose-6-P	NNP
cord-007261-b5fgb9wf	228	2	-	-	HYPH
cord-007261-b5fgb9wf	228	3	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	228	4	was	be	VBD
cord-007261-b5fgb9wf	228	5	resistant	resistant	JJ
cord-007261-b5fgb9wf	228	6	to	to	IN
cord-007261-b5fgb9wf	228	7	the	the	DT
cord-007261-b5fgb9wf	228	8	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	228	9	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	228	10	treatment	treatment	NN
cord-007261-b5fgb9wf	228	11	whereas	whereas	IN
cord-007261-b5fgb9wf	228	12	mannose-6-phosphate	mannose-6-phosphate	NNP
cord-007261-b5fgb9wf	228	13	was	be	VBD
cord-007261-b5fgb9wf	228	14	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	228	15	(	(	-LRB-
cord-007261-b5fgb9wf	228	16	3	3	CD
cord-007261-b5fgb9wf	228	17	,	,	,
cord-007261-b5fgb9wf	228	18	4	4	CD
cord-007261-b5fgb9wf	228	19	)	)	-RRB-
cord-007261-b5fgb9wf	228	20	.	.	.
cord-007261-b5fgb9wf	229	1	The	the	DT
cord-007261-b5fgb9wf	229	2	CathD	CathD	NNP
cord-007261-b5fgb9wf	229	3	product	product	NN
cord-007261-b5fgb9wf	229	4	was	be	VBD
cord-007261-b5fgb9wf	229	5	partially	partially	RB
cord-007261-b5fgb9wf	229	6	resistant	resistant	JJ
cord-007261-b5fgb9wf	229	7	to	to	IN
cord-007261-b5fgb9wf	229	8	the	the	DT
cord-007261-b5fgb9wf	229	9	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	229	10	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	229	11	treatment	treatment	NN
cord-007261-b5fgb9wf	229	12	(	(	-LRB-
cord-007261-b5fgb9wf	229	13	Fig	Fig	NNP
cord-007261-b5fgb9wf	229	14	.	.	NNP
cord-007261-b5fgb9wf	229	15	9	9	CD
cord-007261-b5fgb9wf	229	16	,	,	,
cord-007261-b5fgb9wf	229	17	lane	lane	NN
cord-007261-b5fgb9wf	229	18	2	2	CD
cord-007261-b5fgb9wf	229	19	)	)	-RRB-
cord-007261-b5fgb9wf	229	20	.	.	.
cord-007261-b5fgb9wf	230	1	In	in	IN
cord-007261-b5fgb9wf	230	2	contrast	contrast	NN
cord-007261-b5fgb9wf	230	3	,	,	,
cord-007261-b5fgb9wf	230	4	the	the	DT
cord-007261-b5fgb9wf	230	5	radioactive	radioactive	JJ
cord-007261-b5fgb9wf	230	6	phosphate	phosphate	NN
cord-007261-b5fgb9wf	230	7	incorporated	incorporate	VBN
cord-007261-b5fgb9wf	230	8	into	into	IN
cord-007261-b5fgb9wf	230	9	the	the	DT
cord-007261-b5fgb9wf	230	10	CathD/	cathd/	NN
cord-007261-b5fgb9wf	231	1	Ml	ml	NN
cord-007261-b5fgb9wf	231	2	product	product	NN
cord-007261-b5fgb9wf	231	3	was	be	VBD
cord-007261-b5fgb9wf	231	4	completely	completely	RB
cord-007261-b5fgb9wf	231	5	removed	remove	VBN
cord-007261-b5fgb9wf	231	6	by	by	IN
cord-007261-b5fgb9wf	231	7	the	the	DT
cord-007261-b5fgb9wf	231	8	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	231	9	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	231	10	treatment	treatment	NN
cord-007261-b5fgb9wf	231	11	(	(	-LRB-
cord-007261-b5fgb9wf	231	12	Fig-9	Fig-9	NNP
cord-007261-b5fgb9wf	231	13	,	,	,
cord-007261-b5fgb9wf	231	14	lan	lan	NNP
cord-007261-b5fgb9wf	231	15	e	e	NNP
cord-007261-b5fgb9wf	231	16	6	6	CD
cord-007261-b5fgb9wf	231	17	)	)	-RRB-
cord-007261-b5fgb9wf	231	18	.	.	.
cord-007261-b5fgb9wf	232	1	This	this	DT
cord-007261-b5fgb9wf	232	2	result	result	NN
cord-007261-b5fgb9wf	232	3	apparently	apparently	RB
cord-007261-b5fgb9wf	232	4	indicated	indicate	VBD
cord-007261-b5fgb9wf	232	5	that	that	IN
cord-007261-b5fgb9wf	232	6	the	the	DT
cord-007261-b5fgb9wf	232	7	fusion	fusion	NN
cord-007261-b5fgb9wf	232	8	protein	protein	NN
cord-007261-b5fgb9wf	232	9	did	do	VBD
cord-007261-b5fgb9wf	232	10	not	not	RB
cord-007261-b5fgb9wf	232	11	undergo	undergo	VB
cord-007261-b5fgb9wf	232	12	the	the	DT
cord-007261-b5fgb9wf	232	13	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	232	14	-	-	HYPH
cord-007261-b5fgb9wf	232	15	P	p	NN
cord-007261-b5fgb9wf	232	16	modification	modification	NN
cord-007261-b5fgb9wf	232	17	of	of	IN
cord-007261-b5fgb9wf	232	18	the	the	DT
cord-007261-b5fgb9wf	232	19	mannose	mannose	NN
cord-007261-b5fgb9wf	232	20	in	in	IN
cord-007261-b5fgb9wf	232	21	the	the	DT
cord-007261-b5fgb9wf	232	22	sugar	sugar	NN
cord-007261-b5fgb9wf	232	23	chain	chain	NN
cord-007261-b5fgb9wf	232	24	:	:	:
cord-007261-b5fgb9wf	232	25	the	the	DT
cord-007261-b5fgb9wf	232	26	fusion	fusion	NN
cord-007261-b5fgb9wf	232	27	protein	protein	NN
cord-007261-b5fgb9wf	232	28	did	do	VBD
cord-007261-b5fgb9wf	232	29	not	not	RB
cord-007261-b5fgb9wf	232	30	reach	reach	VB
cord-007261-b5fgb9wf	232	31	the	the	DT
cord-007261-b5fgb9wf	232	32	cis	cis	NNP
cord-007261-b5fgb9wf	232	33	-	-	HYPH
cord-007261-b5fgb9wf	232	34	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	232	35	compartment	compartment	NN
cord-007261-b5fgb9wf	232	36	.	.	.
cord-007261-b5fgb9wf	233	1	However	however	RB
cord-007261-b5fgb9wf	233	2	,	,	,
cord-007261-b5fgb9wf	233	3	there	there	EX
cord-007261-b5fgb9wf	233	4	is	be	VBZ
cord-007261-b5fgb9wf	233	5	a	a	DT
cord-007261-b5fgb9wf	233	6	possibility	possibility	NN
cord-007261-b5fgb9wf	233	7	that	that	IN
cord-007261-b5fgb9wf	233	8	the	the	DT
cord-007261-b5fgb9wf	233	9	conversion	conversion	NN
cord-007261-b5fgb9wf	233	10	of	of	IN
cord-007261-b5fgb9wf	233	11	the	the	DT
cord-007261-b5fgb9wf	233	12	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	233	13	-	-	HYPH
cord-007261-b5fgb9wf	233	14	P	P	NNP
cord-007261-b5fgb9wf	233	15	moiety	moiety	NN
cord-007261-b5fgb9wf	233	16	of	of	IN
cord-007261-b5fgb9wf	233	17	the	the	DT
cord-007261-b5fgb9wf	233	18	fusion	fusion	NN
cord-007261-b5fgb9wf	233	19	protein	protein	NN
cord-007261-b5fgb9wf	233	20	to	to	IN
cord-007261-b5fgb9wf	233	21	M6P	M6P	NNP
cord-007261-b5fgb9wf	233	22	was	be	VBD
cord-007261-b5fgb9wf	233	23	very	very	RB
cord-007261-b5fgb9wf	233	24	rapid	rapid	JJ
cord-007261-b5fgb9wf	233	25	in	in	IN
cord-007261-b5fgb9wf	233	26	the	the	DT
cord-007261-b5fgb9wf	233	27	transfected	transfecte	VBN
cord-007261-b5fgb9wf	233	28	cells	cell	NNS
cord-007261-b5fgb9wf	233	29	,	,	,
cord-007261-b5fgb9wf	233	30	resulting	result	VBG
cord-007261-b5fgb9wf	233	31	in	in	IN
cord-007261-b5fgb9wf	233	32	the	the	DT
cord-007261-b5fgb9wf	233	33	undetectable	undetectable	JJ
cord-007261-b5fgb9wf	233	34	amount	amount	NN
cord-007261-b5fgb9wf	233	35	of	of	IN
cord-007261-b5fgb9wf	233	36	the	the	DT
cord-007261-b5fgb9wf	233	37	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	233	38	-	-	HYPH
cord-007261-b5fgb9wf	233	39	P	p	NN
cord-007261-b5fgb9wf	233	40	intermediate	intermediate	NN
cord-007261-b5fgb9wf	233	41	.	.	.
cord-007261-b5fgb9wf	234	1	However	however	RB
cord-007261-b5fgb9wf	234	2	,	,	,
cord-007261-b5fgb9wf	234	3	such	such	PDT
cord-007261-b5fgb9wf	234	4	a	a	DT
cord-007261-b5fgb9wf	234	5	possibility	possibility	NN
cord-007261-b5fgb9wf	234	6	seems	seem	VBZ
cord-007261-b5fgb9wf	234	7	to	to	TO
cord-007261-b5fgb9wf	234	8	be	be	VB
cord-007261-b5fgb9wf	234	9	very	very	RB
cord-007261-b5fgb9wf	234	10	low	low	JJ
cord-007261-b5fgb9wf	234	11	since	since	IN
cord-007261-b5fgb9wf	234	12	the	the	DT
cord-007261-b5fgb9wf	234	13	expressed	express	VBN
cord-007261-b5fgb9wf	234	14	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	234	15	D	d	NN
cord-007261-b5fgb9wf	234	16	carried	carry	VBD
cord-007261-b5fgb9wf	234	17	a	a	DT
cord-007261-b5fgb9wf	234	18	detectable	detectable	JJ
cord-007261-b5fgb9wf	234	19	amount	amount	NN
cord-007261-b5fgb9wf	234	20	of	of	IN
cord-007261-b5fgb9wf	234	21	the	the	DT
cord-007261-b5fgb9wf	234	22	GlcNAc	GlcNAc	NNP
cord-007261-b5fgb9wf	234	23	-	-	HYPH
cord-007261-b5fgb9wf	234	24	P	p	NN
cord-007261-b5fgb9wf	234	25	intermediate	intermediate	NN
cord-007261-b5fgb9wf	234	26	.	.	.
cord-007261-b5fgb9wf	235	1	Incidentally	incidentally	RB
cord-007261-b5fgb9wf	235	2	,	,	,
cord-007261-b5fgb9wf	235	3	the	the	DT
cord-007261-b5fgb9wf	235	4	radioactive	radioactive	JJ
cord-007261-b5fgb9wf	235	5	phosphate	phosphate	NN
cord-007261-b5fgb9wf	235	6	incorporated	incorporate	VBN
cord-007261-b5fgb9wf	235	7	into	into	IN
cord-007261-b5fgb9wf	235	8	the	the	DT
cord-007261-b5fgb9wf	235	9	Ml	Ml	NNP
cord-007261-b5fgb9wf	235	10	product	product	NN
cord-007261-b5fgb9wf	235	11	was	be	VBD
cord-007261-b5fgb9wf	235	12	also	also	RB
cord-007261-b5fgb9wf	235	13	removed	remove	VBN
cord-007261-b5fgb9wf	235	14	by	by	IN
cord-007261-b5fgb9wf	235	15	the	the	DT
cord-007261-b5fgb9wf	235	16	alkaline	alkaline	JJ
cord-007261-b5fgb9wf	235	17	phosphatase	phosphatase	NN
cord-007261-b5fgb9wf	235	18	treatment	treatment	NN
cord-007261-b5fgb9wf	235	19	.	.	.
cord-007261-b5fgb9wf	236	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	236	2	is	be	VBZ
cord-007261-b5fgb9wf	236	3	known	know	VBN
cord-007261-b5fgb9wf	236	4	that	that	IN
cord-007261-b5fgb9wf	236	5	some	some	DT
cord-007261-b5fgb9wf	236	6	P450s	P450s	NNP
cord-007261-b5fgb9wf	236	7	,	,	,
cord-007261-b5fgb9wf	236	8	including	include	VBG
cord-007261-b5fgb9wf	236	9	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	236	10	)	)	-RRB-
cord-007261-b5fgb9wf	236	11	,	,	,
cord-007261-b5fgb9wf	236	12	are	be	VBP
cord-007261-b5fgb9wf	236	13	phosphorylated	phosphorylate	VBN
cord-007261-b5fgb9wf	236	14	by	by	IN
cord-007261-b5fgb9wf	236	15	cAMP	camp	NN
cord-007261-b5fgb9wf	236	16	dependent	dependent	JJ
cord-007261-b5fgb9wf	236	17	protein	protein	NN
cord-007261-b5fgb9wf	236	18	kinase	kinase	NN
cord-007261-b5fgb9wf	236	19	(	(	-LRB-
cord-007261-b5fgb9wf	236	20	30	30	CD
cord-007261-b5fgb9wf	236	21	)	)	-RRB-
cord-007261-b5fgb9wf	236	22	.	.	.
cord-007261-b5fgb9wf	237	1	Therefore	therefore	RB
cord-007261-b5fgb9wf	237	2	,	,	,
cord-007261-b5fgb9wf	237	3	the	the	DT
cord-007261-b5fgb9wf	237	4	phosphate	phosphate	NN
cord-007261-b5fgb9wf	237	5	incorporation	incorporation	NN
cord-007261-b5fgb9wf	237	6	into	into	IN
cord-007261-b5fgb9wf	237	7	the	the	DT
cord-007261-b5fgb9wf	237	8	CathD	CathD	NNP
cord-007261-b5fgb9wf	237	9	/	/	SYM
cord-007261-b5fgb9wf	237	10	Ml	Ml	NNP
cord-007261-b5fgb9wf	237	11	and	and	CC
cord-007261-b5fgb9wf	237	12	Ml	Ml	NNP
cord-007261-b5fgb9wf	237	13	products	product	NNS
cord-007261-b5fgb9wf	237	14	was	be	VBD
cord-007261-b5fgb9wf	237	15	probably	probably	RB
cord-007261-b5fgb9wf	237	16	due	due	JJ
cord-007261-b5fgb9wf	237	17	to	to	IN
cord-007261-b5fgb9wf	237	18	the	the	DT
cord-007261-b5fgb9wf	237	19	phosphorylation	phosphorylation	NN
cord-007261-b5fgb9wf	237	20	of	of	IN
cord-007261-b5fgb9wf	237	21	the	the	DT
cord-007261-b5fgb9wf	237	22	protein	protein	NN
cord-007261-b5fgb9wf	237	23	moiety	moiety	NN
cord-007261-b5fgb9wf	237	24	by	by	IN
cord-007261-b5fgb9wf	237	25	the	the	DT
cord-007261-b5fgb9wf	237	26	protein	protein	NN
cord-007261-b5fgb9wf	237	27	kinase	kinase	NN
cord-007261-b5fgb9wf	237	28	.	.	.
cord-007261-b5fgb9wf	238	1	The	the	DT
cord-007261-b5fgb9wf	238	2	retention	retention	NN
cord-007261-b5fgb9wf	238	3	mechanism	mechanism	NN
cord-007261-b5fgb9wf	238	4	for	for	IN
cord-007261-b5fgb9wf	238	5	the	the	DT
cord-007261-b5fgb9wf	238	6	ER	ER	NNP
cord-007261-b5fgb9wf	238	7	resident	resident	NN
cord-007261-b5fgb9wf	238	8	membrane	membrane	NN
cord-007261-b5fgb9wf	238	9	proteins	protein	NNS
cord-007261-b5fgb9wf	238	10	is	be	VBZ
cord-007261-b5fgb9wf	238	11	insufficiently	insufficiently	RB
cord-007261-b5fgb9wf	238	12	understood	understand	VBN
cord-007261-b5fgb9wf	238	13	.	.	.
cord-007261-b5fgb9wf	239	1	As	as	IN
cord-007261-b5fgb9wf	239	2	a	a	DT
cord-007261-b5fgb9wf	239	3	step	step	NN
cord-007261-b5fgb9wf	239	4	to	to	TO
cord-007261-b5fgb9wf	239	5	elucidate	elucidate	VB
cord-007261-b5fgb9wf	239	6	the	the	DT
cord-007261-b5fgb9wf	239	7	retention	retention	NN
cord-007261-b5fgb9wf	239	8	mechanism	mechanism	NN
cord-007261-b5fgb9wf	239	9	,	,	,
cord-007261-b5fgb9wf	239	10	we	-PRON-	PRP
cord-007261-b5fgb9wf	239	11	examined	examine	VBD
cord-007261-b5fgb9wf	239	12	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	239	13	P450-(Ml	p450-(ml	$
cord-007261-b5fgb9wf	239	14	)	)	-RRB-
cord-007261-b5fgb9wf	239	15	to	to	TO
cord-007261-b5fgb9wf	239	16	locate	locate	VB
cord-007261-b5fgb9wf	239	17	the	the	DT
cord-007261-b5fgb9wf	239	18	region	region	NN
cord-007261-b5fgb9wf	239	19	in	in	IN
cord-007261-b5fgb9wf	239	20	the	the	DT
cord-007261-b5fgb9wf	239	21	polypeptide	polypeptide	NN
cord-007261-b5fgb9wf	239	22	chain	chain	NN
cord-007261-b5fgb9wf	239	23	responsible	responsible	JJ
cord-007261-b5fgb9wf	239	24	for	for	IN
cord-007261-b5fgb9wf	239	25	its	-PRON-	PRP$
cord-007261-b5fgb9wf	239	26	ER	ER	NNP
cord-007261-b5fgb9wf	239	27	retention	retention	NN
cord-007261-b5fgb9wf	239	28	,	,	,
cord-007261-b5fgb9wf	239	29	and	and	CC
cord-007261-b5fgb9wf	239	30	found	find	VBD
cord-007261-b5fgb9wf	239	31	that	that	IN
cord-007261-b5fgb9wf	239	32	the	the	DT
cord-007261-b5fgb9wf	239	33	17	17	CD
cord-007261-b5fgb9wf	239	34	amino	amino	NN
cord-007261-b5fgb9wf	239	35	acidlong	acidlong	IN
cord-007261-b5fgb9wf	239	36	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	239	37	signal	signal	NN
cord-007261-b5fgb9wf	239	38	-	-	HYPH
cord-007261-b5fgb9wf	239	39	anchor	anchor	NN
cord-007261-b5fgb9wf	239	40	region	region	NN
cord-007261-b5fgb9wf	239	41	of	of	IN
cord-007261-b5fgb9wf	239	42	the	the	DT
cord-007261-b5fgb9wf	239	43	membrane	membrane	NN
cord-007261-b5fgb9wf	239	44	-	-	HYPH
cord-007261-b5fgb9wf	239	45	bound	bind	VBN
cord-007261-b5fgb9wf	239	46	hemoprotein	hemoprotein	NNP
cord-007261-b5fgb9wf	239	47	was	be	VBD
cord-007261-b5fgb9wf	239	48	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	239	49	for	for	IN
cord-007261-b5fgb9wf	239	50	the	the	DT
cord-007261-b5fgb9wf	239	51	retention	retention	NN
cord-007261-b5fgb9wf	239	52	.	.	.
cord-007261-b5fgb9wf	240	1	This	this	DT
cord-007261-b5fgb9wf	240	2	conclusion	conclusion	NN
cord-007261-b5fgb9wf	240	3	was	be	VBD
cord-007261-b5fgb9wf	240	4	based	base	VBN
cord-007261-b5fgb9wf	240	5	on	on	IN
cord-007261-b5fgb9wf	240	6	the	the	DT
cord-007261-b5fgb9wf	240	7	expression	expression	NN
cord-007261-b5fgb9wf	240	8	of	of	IN
cord-007261-b5fgb9wf	240	9	the	the	DT
cord-007261-b5fgb9wf	240	10	fusion	fusion	NN
cord-007261-b5fgb9wf	240	11	proteins	protein	NNS
cord-007261-b5fgb9wf	240	12	consisting	consist	VBG
cord-007261-b5fgb9wf	240	13	of	of	IN
cord-007261-b5fgb9wf	240	14	carboxyesterase	carboxyesterase	NN
cord-007261-b5fgb9wf	240	15	Sec	Sec	NNP
cord-007261-b5fgb9wf	240	16	,	,	,
cord-007261-b5fgb9wf	240	17	a	a	DT
cord-007261-b5fgb9wf	240	18	secretory	secretory	JJ
cord-007261-b5fgb9wf	240	19	protein	protein	NN
cord-007261-b5fgb9wf	240	20	used	use	VBN
cord-007261-b5fgb9wf	240	21	as	as	IN
cord-007261-b5fgb9wf	240	22	a	a	DT
cord-007261-b5fgb9wf	240	23	reporter	reporter	NN
cord-007261-b5fgb9wf	240	24	,	,	,
cord-007261-b5fgb9wf	240	25	and	and	CC
cord-007261-b5fgb9wf	240	26	various	various	JJ
cord-007261-b5fgb9wf	240	27	portions	portion	NNS
cord-007261-b5fgb9wf	240	28	of	of	IN
cord-007261-b5fgb9wf	240	29	P450-(Ml	p450-(ml	CD
cord-007261-b5fgb9wf	240	30	)	)	-RRB-
cord-007261-b5fgb9wf	240	31	in	in	IN
cord-007261-b5fgb9wf	240	32	COS	cos	NN
cord-007261-b5fgb9wf	240	33	cells	cell	NNS
cord-007261-b5fgb9wf	240	34	.	.	.
cord-007261-b5fgb9wf	241	1	The	the	DT
cord-007261-b5fgb9wf	241	2	reporter	reporter	NN
cord-007261-b5fgb9wf	241	3	portion	portion	NN
cord-007261-b5fgb9wf	241	4	of	of	IN
cord-007261-b5fgb9wf	241	5	the	the	DT
cord-007261-b5fgb9wf	241	6	expressed	expressed	JJ
cord-007261-b5fgb9wf	241	7	173	173	CD
cord-007261-b5fgb9wf	241	8	fusion	fusion	NN
cord-007261-b5fgb9wf	241	9	proteins	protein	NNS
cord-007261-b5fgb9wf	241	10	attained	attain	VBD
cord-007261-b5fgb9wf	241	11	the	the	DT
cord-007261-b5fgb9wf	241	12	correct	correct	JJ
cord-007261-b5fgb9wf	241	13	conformation	conformation	NN
cord-007261-b5fgb9wf	241	14	judging	judge	VBG
cord-007261-b5fgb9wf	241	15	from	from	IN
cord-007261-b5fgb9wf	241	16	the	the	DT
cord-007261-b5fgb9wf	241	17	fact	fact	NN
cord-007261-b5fgb9wf	241	18	that	that	IN
cord-007261-b5fgb9wf	241	19	the	the	DT
cord-007261-b5fgb9wf	241	20	fusion	fusion	NN
cord-007261-b5fgb9wf	241	21	proteins	protein	NNS
cord-007261-b5fgb9wf	241	22	were	be	VBD
cord-007261-b5fgb9wf	241	23	enzymatically	enzymatically	RB
cord-007261-b5fgb9wf	241	24	active	active	JJ
cord-007261-b5fgb9wf	241	25	.	.	.
cord-007261-b5fgb9wf	242	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	242	2	was	be	VBD
cord-007261-b5fgb9wf	242	3	therefore	therefore	RB
cord-007261-b5fgb9wf	242	4	concluded	conclude	VBN
cord-007261-b5fgb9wf	242	5	that	that	IN
cord-007261-b5fgb9wf	242	6	the	the	DT
cord-007261-b5fgb9wf	242	7	fusion	fusion	NN
cord-007261-b5fgb9wf	242	8	proteins	protein	NNS
cord-007261-b5fgb9wf	242	9	were	be	VBD
cord-007261-b5fgb9wf	242	10	retained	retain	VBN
cord-007261-b5fgb9wf	242	11	in	in	IN
cord-007261-b5fgb9wf	242	12	the	the	DT
cord-007261-b5fgb9wf	242	13	ER	ER	NNP
cord-007261-b5fgb9wf	242	14	by	by	IN
cord-007261-b5fgb9wf	242	15	the	the	DT
cord-007261-b5fgb9wf	242	16	retention	retention	NN
cord-007261-b5fgb9wf	242	17	signal	signal	NN
cord-007261-b5fgb9wf	242	18	in	in	IN
cord-007261-b5fgb9wf	242	19	the	the	DT
cord-007261-b5fgb9wf	242	20	fusion	fusion	NN
cord-007261-b5fgb9wf	242	21	proteins	protein	NNS
cord-007261-b5fgb9wf	242	22	and	and	CC
cord-007261-b5fgb9wf	242	23	not	not	RB
cord-007261-b5fgb9wf	242	24	through	through	IN
cord-007261-b5fgb9wf	242	25	the	the	DT
cord-007261-b5fgb9wf	242	26	action	action	NN
cord-007261-b5fgb9wf	242	27	of	of	IN
cord-007261-b5fgb9wf	242	28	the	the	DT
cord-007261-b5fgb9wf	242	29	quality	quality	NN
cord-007261-b5fgb9wf	242	30	control	control	NN
cord-007261-b5fgb9wf	242	31	system	system	NN
cord-007261-b5fgb9wf	242	32	for	for	IN
cord-007261-b5fgb9wf	242	33	unfolded	unfolded	JJ
cord-007261-b5fgb9wf	242	34	proteins	protein	NNS
cord-007261-b5fgb9wf	242	35	in	in	IN
cord-007261-b5fgb9wf	242	36	the	the	DT
cord-007261-b5fgb9wf	242	37	ER	ER	NNP
cord-007261-b5fgb9wf	242	38	lumen	lumen	NN
cord-007261-b5fgb9wf	242	39	.	.	.
cord-007261-b5fgb9wf	243	1	Several	several	JJ
cord-007261-b5fgb9wf	243	2	highly	highly	RB
cord-007261-b5fgb9wf	243	3	conserved	conserved	JJ
cord-007261-b5fgb9wf	243	4	structural	structural	JJ
cord-007261-b5fgb9wf	243	5	motifs	motif	NNS
cord-007261-b5fgb9wf	243	6	have	have	VBP
cord-007261-b5fgb9wf	243	7	been	be	VBN
cord-007261-b5fgb9wf	243	8	noted	note	VBN
cord-007261-b5fgb9wf	243	9	in	in	IN
cord-007261-b5fgb9wf	243	10	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	243	11	P450s	P450s	NNP
cord-007261-b5fgb9wf	243	12	;	;	:
cord-007261-b5fgb9wf	243	13	the	the	DT
cord-007261-b5fgb9wf	243	14	basic	basic	JJ
cord-007261-b5fgb9wf	243	15	amino	amino	NN
cord-007261-b5fgb9wf	243	16	acid	acid	NN
cord-007261-b5fgb9wf	243	17	-	-	HYPH
cord-007261-b5fgb9wf	243	18	rich	rich	JJ
cord-007261-b5fgb9wf	243	19	region	region	NN
cord-007261-b5fgb9wf	243	20	at	at	IN
cord-007261-b5fgb9wf	243	21	the	the	DT
cord-007261-b5fgb9wf	243	22	C	C	NNP
cord-007261-b5fgb9wf	243	23	-	-	HYPH
cord-007261-b5fgb9wf	243	24	terminus	terminus	NN
cord-007261-b5fgb9wf	243	25	of	of	IN
cord-007261-b5fgb9wf	243	26	the	the	DT
cord-007261-b5fgb9wf	243	27	signal	signal	NN
cord-007261-b5fgb9wf	243	28	-	-	HYPH
cord-007261-b5fgb9wf	243	29	anchor	anchor	NN
cord-007261-b5fgb9wf	243	30	sequence	sequence	NN
cord-007261-b5fgb9wf	243	31	,	,	,
cord-007261-b5fgb9wf	243	32	the	the	DT
cord-007261-b5fgb9wf	243	33	proline	proline	NN
cord-007261-b5fgb9wf	243	34	-	-	HYPH
cord-007261-b5fgb9wf	243	35	rich	rich	JJ
cord-007261-b5fgb9wf	243	36	region	region	NN
cord-007261-b5fgb9wf	243	37	following	follow	VBG
cord-007261-b5fgb9wf	243	38	the	the	DT
cord-007261-b5fgb9wf	243	39	basic	basic	JJ
cord-007261-b5fgb9wf	243	40	amino	amino	NN
cord-007261-b5fgb9wf	243	41	acid	acid	NN
cord-007261-b5fgb9wf	243	42	-	-	HYPH
cord-007261-b5fgb9wf	243	43	rich	rich	JJ
cord-007261-b5fgb9wf	243	44	region	region	NN
cord-007261-b5fgb9wf	243	45	,	,	,
cord-007261-b5fgb9wf	243	46	the	the	DT
cord-007261-b5fgb9wf	243	47	heme	heme	NN
cord-007261-b5fgb9wf	243	48	binding	bind	VBG
cord-007261-b5fgb9wf	243	49	region	region	NN
cord-007261-b5fgb9wf	243	50	containing	contain	VBG
cord-007261-b5fgb9wf	243	51	a	a	DT
cord-007261-b5fgb9wf	243	52	conserved	conserve	VBN
cord-007261-b5fgb9wf	243	53	cysteine	cysteine	NN
cord-007261-b5fgb9wf	243	54	residue	residue	NN
cord-007261-b5fgb9wf	243	55	,	,	,
cord-007261-b5fgb9wf	243	56	and	and	CC
cord-007261-b5fgb9wf	243	57	the	the	DT
cord-007261-b5fgb9wf	243	58	threonine	threonine	JJ
cord-007261-b5fgb9wf	243	59	-	-	HYPH
cord-007261-b5fgb9wf	243	60	serine	serine	NN
cord-007261-b5fgb9wf	243	61	cluster	cluster	NN
cord-007261-b5fgb9wf	243	62	in	in	IN
cord-007261-b5fgb9wf	243	63	the	the	DT
cord-007261-b5fgb9wf	243	64	middle	middle	JJ
cord-007261-b5fgb9wf	243	65	portion	portion	NN
cord-007261-b5fgb9wf	243	66	of	of	IN
cord-007261-b5fgb9wf	243	67	the	the	DT
cord-007261-b5fgb9wf	243	68	molecule	molecule	NN
cord-007261-b5fgb9wf	243	69	.	.	.
cord-007261-b5fgb9wf	244	1	However	however	RB
cord-007261-b5fgb9wf	244	2	,	,	,
cord-007261-b5fgb9wf	244	3	these	these	DT
cord-007261-b5fgb9wf	244	4	common	common	JJ
cord-007261-b5fgb9wf	244	5	structural	structural	JJ
cord-007261-b5fgb9wf	244	6	motifs	motif	NNS
cord-007261-b5fgb9wf	244	7	seem	seem	VBP
cord-007261-b5fgb9wf	244	8	to	to	TO
cord-007261-b5fgb9wf	244	9	have	have	VB
cord-007261-b5fgb9wf	244	10	nothing	nothing	NN
cord-007261-b5fgb9wf	244	11	to	to	TO
cord-007261-b5fgb9wf	244	12	do	do	VB
cord-007261-b5fgb9wf	244	13	with	with	IN
cord-007261-b5fgb9wf	244	14	the	the	DT
cord-007261-b5fgb9wf	244	15	ER	ER	NNP
cord-007261-b5fgb9wf	244	16	retention	retention	NN
cord-007261-b5fgb9wf	244	17	of	of	IN
cord-007261-b5fgb9wf	244	18	the	the	DT
cord-007261-b5fgb9wf	244	19	P450s	P450s	NNP
cord-007261-b5fgb9wf	244	20	.	.	.
cord-007261-b5fgb9wf	245	1	When	when	WRB
cord-007261-b5fgb9wf	245	2	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	245	3	Sec	Sec	NNP
cord-007261-b5fgb9wf	245	4	was	be	VBD
cord-007261-b5fgb9wf	245	5	fused	fuse	VBN
cord-007261-b5fgb9wf	245	6	with	with	IN
cord-007261-b5fgb9wf	245	7	the	the	DT
cord-007261-b5fgb9wf	245	8	carboxyterminal	carboxyterminal	JJ
cord-007261-b5fgb9wf	245	9	portion	portion	NN
cord-007261-b5fgb9wf	245	10	of	of	IN
cord-007261-b5fgb9wf	245	11	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	245	12	UDP	udp	NN
cord-007261-b5fgb9wf	245	13	-	-	HYPH
cord-007261-b5fgb9wf	245	14	glucuronosyltransferase	glucuronosyltransferase	NNP
cord-007261-b5fgb9wf	245	15	(	(	-LRB-
cord-007261-b5fgb9wf	245	16	GT	GT	NNP
cord-007261-b5fgb9wf	245	17	)	)	-RRB-
cord-007261-b5fgb9wf	245	18	consisting	consist	VBG
cord-007261-b5fgb9wf	245	19	of	of	IN
cord-007261-b5fgb9wf	245	20	a	a	DT
cord-007261-b5fgb9wf	245	21	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	245	22	segment	segment	NN
cord-007261-b5fgb9wf	245	23	and	and	CC
cord-007261-b5fgb9wf	245	24	a	a	DT
cord-007261-b5fgb9wf	245	25	short	short	JJ
cord-007261-b5fgb9wf	245	26	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	245	27	tail	tail	NN
cord-007261-b5fgb9wf	245	28	,	,	,
cord-007261-b5fgb9wf	245	29	the	the	DT
cord-007261-b5fgb9wf	245	30	fusion	fusion	NN
cord-007261-b5fgb9wf	245	31	protein	protein	NN
cord-007261-b5fgb9wf	245	32	expressed	express	VBD
cord-007261-b5fgb9wf	245	33	in	in	IN
cord-007261-b5fgb9wf	245	34	COS	COS	NNP
cord-007261-b5fgb9wf	245	35	cells	cell	NNS
cord-007261-b5fgb9wf	245	36	was	be	VBD
cord-007261-b5fgb9wf	245	37	retained	retain	VBN
cord-007261-b5fgb9wf	245	38	in	in	IN
cord-007261-b5fgb9wf	245	39	the	the	DT
cord-007261-b5fgb9wf	245	40	ER	ER	NNP
cord-007261-b5fgb9wf	245	41	,	,	,
cord-007261-b5fgb9wf	245	42	indicating	indicate	VBG
cord-007261-b5fgb9wf	245	43	that	that	IN
cord-007261-b5fgb9wf	245	44	the	the	DT
cord-007261-b5fgb9wf	245	45	doublelysine	doublelysine	NN
cord-007261-b5fgb9wf	245	46	motif	motif	NN
cord-007261-b5fgb9wf	245	47	in	in	IN
cord-007261-b5fgb9wf	245	48	the	the	DT
cord-007261-b5fgb9wf	245	49	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	245	50	tail	tail	NN
cord-007261-b5fgb9wf	245	51	functioned	function	VBD
cord-007261-b5fgb9wf	245	52	as	as	IN
cord-007261-b5fgb9wf	245	53	the	the	DT
cord-007261-b5fgb9wf	245	54	ER	ER	NNP
cord-007261-b5fgb9wf	245	55	retention	retention	NN
cord-007261-b5fgb9wf	245	56	signal	signal	NN
cord-007261-b5fgb9wf	245	57	.	.	.
cord-007261-b5fgb9wf	246	1	In	in	IN
cord-007261-b5fgb9wf	246	2	contrast	contrast	NN
cord-007261-b5fgb9wf	246	3	,	,	,
cord-007261-b5fgb9wf	246	4	another	another	DT
cord-007261-b5fgb9wf	246	5	fusion	fusion	NN
cord-007261-b5fgb9wf	246	6	protein	protein	NN
cord-007261-b5fgb9wf	246	7	in	in	IN
cord-007261-b5fgb9wf	246	8	which	which	WDT
cord-007261-b5fgb9wf	246	9	the	the	DT
cord-007261-b5fgb9wf	246	10	double	double	JJ
cord-007261-b5fgb9wf	246	11	lysine	lysine	NN
cord-007261-b5fgb9wf	246	12	motif	motif	NN
cord-007261-b5fgb9wf	246	13	was	be	VBD
cord-007261-b5fgb9wf	246	14	deleted	delete	VBN
cord-007261-b5fgb9wf	246	15	,	,	,
cord-007261-b5fgb9wf	246	16	Sec	Sec	NNP
cord-007261-b5fgb9wf	246	17	/	/	SYM
cord-007261-b5fgb9wf	246	18	GTd	GTd	NNS
cord-007261-b5fgb9wf	246	19	,	,	,
cord-007261-b5fgb9wf	246	20	was	be	VBD
cord-007261-b5fgb9wf	246	21	transported	transport	VBN
cord-007261-b5fgb9wf	246	22	from	from	IN
cord-007261-b5fgb9wf	246	23	the	the	DT
cord-007261-b5fgb9wf	246	24	ER	ER	NNP
cord-007261-b5fgb9wf	246	25	and	and	CC
cord-007261-b5fgb9wf	246	26	retained	retain	VBN
cord-007261-b5fgb9wf	246	27	in	in	IN
cord-007261-b5fgb9wf	246	28	the	the	DT
cord-007261-b5fgb9wf	246	29	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	246	30	apparatus	apparatus	NN
cord-007261-b5fgb9wf	246	31	.	.	.
cord-007261-b5fgb9wf	247	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	247	2	is	be	VBZ
cord-007261-b5fgb9wf	247	3	known	know	VBN
cord-007261-b5fgb9wf	247	4	that	that	IN
cord-007261-b5fgb9wf	247	5	some	some	DT
cord-007261-b5fgb9wf	247	6	ER	ER	NNP
cord-007261-b5fgb9wf	247	7	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	247	8	proteins	protein	NNS
cord-007261-b5fgb9wf	247	9	retained	retain	VBN
cord-007261-b5fgb9wf	247	10	in	in	IN
cord-007261-b5fgb9wf	247	11	the	the	DT
cord-007261-b5fgb9wf	247	12	ER	ER	NNP
cord-007261-b5fgb9wf	247	13	by	by	IN
cord-007261-b5fgb9wf	247	14	the	the	DT
cord-007261-b5fgb9wf	247	15	double	double	JJ
cord-007261-b5fgb9wf	247	16	lysine	lysine	NN
cord-007261-b5fgb9wf	247	17	motif	motif	NN
cord-007261-b5fgb9wf	247	18	are	be	VBP
cord-007261-b5fgb9wf	247	19	transported	transport	VBN
cord-007261-b5fgb9wf	247	20	to	to	IN
cord-007261-b5fgb9wf	247	21	the	the	DT
cord-007261-b5fgb9wf	247	22	plasma	plasma	NN
cord-007261-b5fgb9wf	247	23	membrane	membrane	NN
cord-007261-b5fgb9wf	247	24	on	on	IN
cord-007261-b5fgb9wf	247	25	deletion	deletion	NN
cord-007261-b5fgb9wf	247	26	of	of	IN
cord-007261-b5fgb9wf	247	27	the	the	DT
cord-007261-b5fgb9wf	247	28	retention	retention	NN
cord-007261-b5fgb9wf	247	29	motif	motif	NN
cord-007261-b5fgb9wf	247	30	(	(	-LRB-
cord-007261-b5fgb9wf	247	31	7	7	CD
cord-007261-b5fgb9wf	247	32	)	)	-RRB-
cord-007261-b5fgb9wf	247	33	.	.	.
cord-007261-b5fgb9wf	248	1	The	the	DT
cord-007261-b5fgb9wf	248	2	reason	reason	NN
cord-007261-b5fgb9wf	248	3	why	why	WRB
cord-007261-b5fgb9wf	248	4	the	the	DT
cord-007261-b5fgb9wf	248	5	Sec	Sec	NNP
cord-007261-b5fgb9wf	248	6	/	/	SYM
cord-007261-b5fgb9wf	248	7	GTd	gtd	NN
cord-007261-b5fgb9wf	248	8	product	product	NN
cord-007261-b5fgb9wf	248	9	was	be	VBD
cord-007261-b5fgb9wf	248	10	not	not	RB
cord-007261-b5fgb9wf	248	11	transported	transport	VBN
cord-007261-b5fgb9wf	248	12	to	to	IN
cord-007261-b5fgb9wf	248	13	the	the	DT
cord-007261-b5fgb9wf	248	14	plasma	plasma	NN
cord-007261-b5fgb9wf	248	15	membrane	membrane	NN
cord-007261-b5fgb9wf	248	16	is	be	VBZ
cord-007261-b5fgb9wf	248	17	not	not	RB
cord-007261-b5fgb9wf	248	18	clear	clear	JJ
cord-007261-b5fgb9wf	248	19	.	.	.
cord-007261-b5fgb9wf	249	1	Anyhow	anyhow	RB
cord-007261-b5fgb9wf	249	2	,	,	,
cord-007261-b5fgb9wf	249	3	these	these	DT
cord-007261-b5fgb9wf	249	4	results	result	NNS
cord-007261-b5fgb9wf	249	5	indicate	indicate	VBP
cord-007261-b5fgb9wf	249	6	that	that	IN
cord-007261-b5fgb9wf	249	7	the	the	DT
cord-007261-b5fgb9wf	249	8	carboxyesterase	carboxyesterase	JJ
cord-007261-b5fgb9wf	249	9	Sec	Sec	NNP
cord-007261-b5fgb9wf	249	10	portion	portion	NN
cord-007261-b5fgb9wf	249	11	of	of	IN
cord-007261-b5fgb9wf	249	12	the	the	DT
cord-007261-b5fgb9wf	249	13	fusion	fusion	NN
cord-007261-b5fgb9wf	249	14	proteins	protein	NNS
cord-007261-b5fgb9wf	249	15	did	do	VBD
cord-007261-b5fgb9wf	249	16	not	not	RB
cord-007261-b5fgb9wf	249	17	prevent	prevent	VB
cord-007261-b5fgb9wf	249	18	the	the	DT
cord-007261-b5fgb9wf	249	19	proteins	protein	NNS
cord-007261-b5fgb9wf	249	20	from	from	IN
cord-007261-b5fgb9wf	249	21	being	be	VBG
cord-007261-b5fgb9wf	249	22	transported	transport	VBN
cord-007261-b5fgb9wf	249	23	out	out	IN
cord-007261-b5fgb9wf	249	24	of	of	IN
cord-007261-b5fgb9wf	249	25	the	the	DT
cord-007261-b5fgb9wf	249	26	ER	ER	NNP
cord-007261-b5fgb9wf	249	27	.	.	.
cord-007261-b5fgb9wf	250	1	It	-PRON-	PRP
cord-007261-b5fgb9wf	250	2	is	be	VBZ
cord-007261-b5fgb9wf	250	3	well	well	RB
cord-007261-b5fgb9wf	250	4	documented	document	VBN
cord-007261-b5fgb9wf	250	5	that	that	IN
cord-007261-b5fgb9wf	250	6	the	the	DT
cord-007261-b5fgb9wf	250	7	ER	ER	NNP
cord-007261-b5fgb9wf	250	8	luminal	luminal	JJ
cord-007261-b5fgb9wf	250	9	proteins	protein	NNS
cord-007261-b5fgb9wf	250	10	are	be	VBP
cord-007261-b5fgb9wf	250	11	retained	retain	VBN
cord-007261-b5fgb9wf	250	12	in	in	IN
cord-007261-b5fgb9wf	250	13	the	the	DT
cord-007261-b5fgb9wf	250	14	ER	ER	NNP
cord-007261-b5fgb9wf	250	15	through	through	IN
cord-007261-b5fgb9wf	250	16	the	the	DT
cord-007261-b5fgb9wf	250	17	"	"	``
cord-007261-b5fgb9wf	250	18	export	export	NN
cord-007261-b5fgb9wf	250	19	and	and	CC
cord-007261-b5fgb9wf	250	20	retrieval	retrieval	NN
cord-007261-b5fgb9wf	250	21	"	"	''
cord-007261-b5fgb9wf	250	22	mechanism	mechanism	NN
cord-007261-b5fgb9wf	250	23	(	(	-LRB-
cord-007261-b5fgb9wf	250	24	31	31	CD
cord-007261-b5fgb9wf	250	25	,	,	,
cord-007261-b5fgb9wf	250	26	32	32	CD
cord-007261-b5fgb9wf	250	27	)	)	-RRB-
cord-007261-b5fgb9wf	250	28	.	.	.
cord-007261-b5fgb9wf	251	1	A	a	DT
cord-007261-b5fgb9wf	251	2	similar	similar	JJ
cord-007261-b5fgb9wf	251	3	mechanism	mechanism	NN
cord-007261-b5fgb9wf	251	4	is	be	VBZ
cord-007261-b5fgb9wf	251	5	probably	probably	RB
cord-007261-b5fgb9wf	251	6	operative	operative	JJ
cord-007261-b5fgb9wf	251	7	in	in	IN
cord-007261-b5fgb9wf	251	8	the	the	DT
cord-007261-b5fgb9wf	251	9	ER	ER	NNP
cord-007261-b5fgb9wf	251	10	retention	retention	NN
cord-007261-b5fgb9wf	251	11	of	of	IN
cord-007261-b5fgb9wf	251	12	type	type	NN
cord-007261-b5fgb9wf	251	13	I	-PRON-	PRP
cord-007261-b5fgb9wf	251	14	integral	integral	JJ
cord-007261-b5fgb9wf	251	15	membrane	membrane	NN
cord-007261-b5fgb9wf	251	16	proteins	protein	NNS
cord-007261-b5fgb9wf	251	17	with	with	IN
cord-007261-b5fgb9wf	251	18	the	the	DT
cord-007261-b5fgb9wf	251	19	C	C	NNP
cord-007261-b5fgb9wf	251	20	-	-	HYPH
cord-007261-b5fgb9wf	251	21	terminal	terminal	NN
cord-007261-b5fgb9wf	251	22	double	double	JJ
cord-007261-b5fgb9wf	251	23	lysine	lysine	NN
cord-007261-b5fgb9wf	251	24	motif	motif	NN
cord-007261-b5fgb9wf	251	25	,	,	,
cord-007261-b5fgb9wf	251	26	although	although	IN
cord-007261-b5fgb9wf	251	27	the	the	DT
cord-007261-b5fgb9wf	251	28	detailed	detailed	JJ
cord-007261-b5fgb9wf	251	29	mechanism	mechanism	NN
cord-007261-b5fgb9wf	251	30	remains	remain	VBZ
cord-007261-b5fgb9wf	251	31	obscure	obscure	JJ
cord-007261-b5fgb9wf	251	32	in	in	IN
cord-007261-b5fgb9wf	251	33	the	the	DT
cord-007261-b5fgb9wf	251	34	latter	latter	JJ
cord-007261-b5fgb9wf	251	35	cases	case	NNS
cord-007261-b5fgb9wf	251	36	.	.	.
cord-007261-b5fgb9wf	252	1	We	-PRON-	PRP
cord-007261-b5fgb9wf	252	2	addressed	address	VBD
cord-007261-b5fgb9wf	252	3	this	this	DT
cord-007261-b5fgb9wf	252	4	point	point	NN
cord-007261-b5fgb9wf	252	5	for	for	IN
cord-007261-b5fgb9wf	252	6	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	252	7	P450s	P450s	NNP
cord-007261-b5fgb9wf	252	8	using	use	VBG
cord-007261-b5fgb9wf	252	9	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	252	10	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	252	11	D	d	NN
cord-007261-b5fgb9wf	252	12	as	as	IN
cord-007261-b5fgb9wf	252	13	the	the	DT
cord-007261-b5fgb9wf	252	14	reporter	reporter	NN
cord-007261-b5fgb9wf	252	15	protein	protein	NN
cord-007261-b5fgb9wf	252	16	to	to	TO
cord-007261-b5fgb9wf	252	17	monitor	monitor	VB
cord-007261-b5fgb9wf	252	18	the	the	DT
cord-007261-b5fgb9wf	252	19	ER	ER	NNP
cord-007261-b5fgb9wf	252	20	to	to	IN
cord-007261-b5fgb9wf	252	21	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	252	22	traffic	traffic	NN
cord-007261-b5fgb9wf	252	23	in	in	IN
cord-007261-b5fgb9wf	252	24	the	the	DT
cord-007261-b5fgb9wf	252	25	cell	cell	NN
cord-007261-b5fgb9wf	252	26	.	.	.
cord-007261-b5fgb9wf	253	1	The	the	DT
cord-007261-b5fgb9wf	253	2	carbohydrate	carbohydrate	NN
cord-007261-b5fgb9wf	253	3	moiety	moiety	NN
cord-007261-b5fgb9wf	253	4	of	of	IN
cord-007261-b5fgb9wf	253	5	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	253	6	D	D	NNP
cord-007261-b5fgb9wf	253	7	underwent	undergo	VBD
cord-007261-b5fgb9wf	253	8	mannose-6-phosphate	mannose-6-phosphate	JJ
cord-007261-b5fgb9wf	253	9	modification	modification	NN
cord-007261-b5fgb9wf	253	10	in	in	IN
cord-007261-b5fgb9wf	253	11	the	the	DT
cord-007261-b5fgb9wf	253	12	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	253	13	compartments	compartment	NNS
cord-007261-b5fgb9wf	253	14	,	,	,
cord-007261-b5fgb9wf	253	15	but	but	CC
cord-007261-b5fgb9wf	253	16	the	the	DT
cord-007261-b5fgb9wf	253	17	cathepsin	cathepsin	NN
cord-007261-b5fgb9wf	253	18	D	d	NN
cord-007261-b5fgb9wf	253	19	-	-	HYPH
cord-007261-b5fgb9wf	253	20	P45O(M1	p45o(m1	NN
cord-007261-b5fgb9wf	253	21	)	)	-RRB-
cord-007261-b5fgb9wf	253	22	fusion	fusion	NN
cord-007261-b5fgb9wf	253	23	protein	protein	NN
cord-007261-b5fgb9wf	253	24	expressed	express	VBN
cord-007261-b5fgb9wf	253	25	in	in	IN
cord-007261-b5fgb9wf	253	26	COS	COS	NNP
cord-007261-b5fgb9wf	253	27	cells	cell	NNS
cord-007261-b5fgb9wf	253	28	was	be	VBD
cord-007261-b5fgb9wf	253	29	retained	retain	VBN
cord-007261-b5fgb9wf	253	30	in	in	IN
cord-007261-b5fgb9wf	253	31	the	the	DT
cord-007261-b5fgb9wf	253	32	ER	ER	NNP
cord-007261-b5fgb9wf	253	33	and	and	CC
cord-007261-b5fgb9wf	253	34	showed	show	VBD
cord-007261-b5fgb9wf	253	35	no	no	DT
cord-007261-b5fgb9wf	253	36	Golgi	golgi	NN
cord-007261-b5fgb9wf	253	37	-	-	HYPH
cord-007261-b5fgb9wf	253	38	type	type	NN
cord-007261-b5fgb9wf	253	39	modification	modification	NN
cord-007261-b5fgb9wf	253	40	of	of	IN
cord-007261-b5fgb9wf	253	41	the	the	DT
cord-007261-b5fgb9wf	253	42	sugar	sugar	NN
cord-007261-b5fgb9wf	253	43	chains	chain	NNS
cord-007261-b5fgb9wf	253	44	,	,	,
cord-007261-b5fgb9wf	253	45	providing	provide	VBG
cord-007261-b5fgb9wf	253	46	evidence	evidence	NN
cord-007261-b5fgb9wf	253	47	that	that	IN
cord-007261-b5fgb9wf	253	48	the	the	DT
cord-007261-b5fgb9wf	253	49	fusion	fusion	NN
cord-007261-b5fgb9wf	253	50	protein	protein	NN
cord-007261-b5fgb9wf	253	51	,	,	,
cord-007261-b5fgb9wf	253	52	and	and	CC
cord-007261-b5fgb9wf	253	53	probably	probably	RB
cord-007261-b5fgb9wf	253	54	P450s	P450s	NNP
cord-007261-b5fgb9wf	253	55	too	too	RB
cord-007261-b5fgb9wf	253	56	,	,	,
cord-007261-b5fgb9wf	253	57	is	be	VBZ
cord-007261-b5fgb9wf	253	58	not	not	RB
cord-007261-b5fgb9wf	253	59	recycled	recycle	VBN
cord-007261-b5fgb9wf	253	60	through	through	IN
cord-007261-b5fgb9wf	253	61	the	the	DT
cord-007261-b5fgb9wf	253	62	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	253	63	compartments	compartment	NNS
cord-007261-b5fgb9wf	253	64	.	.	.
cord-007261-b5fgb9wf	254	1	These	these	DT
cord-007261-b5fgb9wf	254	2	results	result	NNS
cord-007261-b5fgb9wf	254	3	seem	seem	VBP
cord-007261-b5fgb9wf	254	4	to	to	TO
cord-007261-b5fgb9wf	254	5	be	be	VB
cord-007261-b5fgb9wf	254	6	consistent	consistent	JJ
cord-007261-b5fgb9wf	254	7	with	with	IN
cord-007261-b5fgb9wf	254	8	the	the	DT
cord-007261-b5fgb9wf	254	9	previous	previous	JJ
cord-007261-b5fgb9wf	254	10	observation	observation	NN
cord-007261-b5fgb9wf	254	11	that	that	IN
cord-007261-b5fgb9wf	254	12	P450(PB	P450(PB	NNP
cord-007261-b5fgb9wf	254	13	)	)	-RRB-
cord-007261-b5fgb9wf	254	14	was	be	VBD
cord-007261-b5fgb9wf	254	15	not	not	RB
cord-007261-b5fgb9wf	254	16	detected	detect	VBN
cord-007261-b5fgb9wf	254	17	in	in	IN
cord-007261-b5fgb9wf	254	18	the	the	DT
cord-007261-b5fgb9wf	254	19	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	254	20	membrane	membrane	NN
cord-007261-b5fgb9wf	254	21	immunoelectronmicroscopically	immunoelectronmicroscopically	RB
cord-007261-b5fgb9wf	254	22	,	,	,
cord-007261-b5fgb9wf	254	23	even	even	RB
cord-007261-b5fgb9wf	254	24	after	after	IN
cord-007261-b5fgb9wf	254	25	maximal	maximal	JJ
cord-007261-b5fgb9wf	254	26	induction	induction	NN
cord-007261-b5fgb9wf	254	27	of	of	IN
cord-007261-b5fgb9wf	254	28	the	the	DT
cord-007261-b5fgb9wf	254	29	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	254	30	by	by	IN
cord-007261-b5fgb9wf	254	31	phenobarbital	phenobarbital	NNP
cord-007261-b5fgb9wf	254	32	administration	administration	NNP
cord-007261-b5fgb9wf	254	33	(	(	-LRB-
cord-007261-b5fgb9wf	254	34	33	33	CD
cord-007261-b5fgb9wf	254	35	)	)	-RRB-
cord-007261-b5fgb9wf	254	36	.	.	.
cord-007261-b5fgb9wf	255	1	Recently	recently	RB
cord-007261-b5fgb9wf	255	2	,	,	,
cord-007261-b5fgb9wf	255	3	Szczesna	Szczesna	NNP
cord-007261-b5fgb9wf	255	4	-	-	HYPH
cord-007261-b5fgb9wf	255	5	Skorupa	Skorupa	NNP
cord-007261-b5fgb9wf	255	6	and	and	CC
cord-007261-b5fgb9wf	255	7	Kemper	Kemper	NNP
cord-007261-b5fgb9wf	255	8	(	(	-LRB-
cord-007261-b5fgb9wf	255	9	34	34	CD
cord-007261-b5fgb9wf	255	10	)	)	-RRB-
cord-007261-b5fgb9wf	255	11	reported	report	VBD
cord-007261-b5fgb9wf	255	12	that	that	IN
cord-007261-b5fgb9wf	255	13	P450	p450	JJ
cord-007261-b5fgb9wf	255	14	2C2	2c2	CD
cord-007261-b5fgb9wf	255	15	,	,	,
cord-007261-b5fgb9wf	255	16	which	which	WDT
cord-007261-b5fgb9wf	255	17	was	be	VBD
cord-007261-b5fgb9wf	255	18	localized	localize	VBN
cord-007261-b5fgb9wf	255	19	in	in	IN
cord-007261-b5fgb9wf	255	20	the	the	DT
cord-007261-b5fgb9wf	255	21	ER	ER	NNP
cord-007261-b5fgb9wf	255	22	when	when	WRB
cord-007261-b5fgb9wf	255	23	expressed	express	VBN
cord-007261-b5fgb9wf	255	24	in	in	IN
cord-007261-b5fgb9wf	255	25	COS	COS	NNP
cord-007261-b5fgb9wf	255	26	cells	cell	NNS
cord-007261-b5fgb9wf	255	27	,	,	,
cord-007261-b5fgb9wf	255	28	did	do	VBD
cord-007261-b5fgb9wf	255	29	not	not	RB
cord-007261-b5fgb9wf	255	30	show	show	VB
cord-007261-b5fgb9wf	255	31	any	any	DT
cord-007261-b5fgb9wf	255	32	shift	shift	NN
cord-007261-b5fgb9wf	255	33	in	in	IN
cord-007261-b5fgb9wf	255	34	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	255	35	distribution	distribution	NN
cord-007261-b5fgb9wf	255	36	when	when	WRB
cord-007261-b5fgb9wf	255	37	the	the	DT
cord-007261-b5fgb9wf	255	38	cells	cell	NNS
cord-007261-b5fgb9wf	255	39	were	be	VBD
cord-007261-b5fgb9wf	255	40	incubated	incubate	VBN
cord-007261-b5fgb9wf	255	41	at	at	IN
cord-007261-b5fgb9wf	255	42	low	low	JJ
cord-007261-b5fgb9wf	255	43	temperatures	temperature	NNS
cord-007261-b5fgb9wf	255	44	,	,	,
cord-007261-b5fgb9wf	255	45	at	at	IN
cord-007261-b5fgb9wf	255	46	which	which	WDT
cord-007261-b5fgb9wf	255	47	some	some	DT
cord-007261-b5fgb9wf	255	48	membrane	membrane	NN
cord-007261-b5fgb9wf	255	49	bound	bind	VBN
cord-007261-b5fgb9wf	255	50	ER	er	NN
cord-007261-b5fgb9wf	255	51	proteins	protein	NNS
cord-007261-b5fgb9wf	255	52	have	have	VBP
cord-007261-b5fgb9wf	255	53	been	be	VBN
cord-007261-b5fgb9wf	255	54	shown	show	VBN
cord-007261-b5fgb9wf	255	55	to	to	TO
cord-007261-b5fgb9wf	255	56	shift	shift	VB
cord-007261-b5fgb9wf	255	57	to	to	IN
cord-007261-b5fgb9wf	255	58	the	the	DT
cord-007261-b5fgb9wf	255	59	intermediate	intermediate	JJ
cord-007261-b5fgb9wf	255	60	or	or	CC
cord-007261-b5fgb9wf	255	61	cis	cis	NNP
cord-007261-b5fgb9wf	255	62	-	-	HYPH
cord-007261-b5fgb9wf	255	63	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	255	64	apparatus	apparatus	NN
cord-007261-b5fgb9wf	255	65	.	.	.
cord-007261-b5fgb9wf	256	1	They	-PRON-	PRP
cord-007261-b5fgb9wf	256	2	also	also	RB
cord-007261-b5fgb9wf	256	3	showed	show	VBD
cord-007261-b5fgb9wf	256	4	that	that	IN
cord-007261-b5fgb9wf	256	5	a	a	DT
cord-007261-b5fgb9wf	256	6	modified	modify	VBN
cord-007261-b5fgb9wf	256	7	P450	p450	NN
cord-007261-b5fgb9wf	256	8	2C2	2c2	CD
cord-007261-b5fgb9wf	256	9	with	with	IN
cord-007261-b5fgb9wf	256	10	an	an	DT
cord-007261-b5fgb9wf	256	11	artificially	artificially	RB
cord-007261-b5fgb9wf	256	12	added	add	VBN
cord-007261-b5fgb9wf	256	13	iV	iv	NN
cord-007261-b5fgb9wf	256	14	-	-	HYPH
cord-007261-b5fgb9wf	256	15	glycosylation	glycosylation	NN
cord-007261-b5fgb9wf	256	16	site	site	NN
cord-007261-b5fgb9wf	256	17	at	at	IN
cord-007261-b5fgb9wf	256	18	its	-PRON-	PRP$
cord-007261-b5fgb9wf	256	19	N	n	CD
cord-007261-b5fgb9wf	256	20	-	-	HYPH
cord-007261-b5fgb9wf	256	21	terminus	terminus	NN
cord-007261-b5fgb9wf	256	22	received	receive	VBD
cord-007261-b5fgb9wf	256	23	a	a	DT
cord-007261-b5fgb9wf	256	24	sugar	sugar	NN
cord-007261-b5fgb9wf	256	25	chain	chain	NN
cord-007261-b5fgb9wf	256	26	which	which	WDT
cord-007261-b5fgb9wf	256	27	was	be	VBD
cord-007261-b5fgb9wf	256	28	sensitive	sensitive	JJ
cord-007261-b5fgb9wf	256	29	to	to	IN
cord-007261-b5fgb9wf	256	30	treatment	treatment	NN
cord-007261-b5fgb9wf	256	31	with	with	IN
cord-007261-b5fgb9wf	256	32	endo	endo	NN
cord-007261-b5fgb9wf	256	33	H	H	NNP
cord-007261-b5fgb9wf	256	34	or	or	CC
cord-007261-b5fgb9wf	256	35	endo	endo	NN
cord-007261-b5fgb9wf	256	36	D	d	NN
cord-007261-b5fgb9wf	256	37	,	,	,
cord-007261-b5fgb9wf	256	38	which	which	WDT
cord-007261-b5fgb9wf	256	39	indicated	indicate	VBD
cord-007261-b5fgb9wf	256	40	that	that	IN
cord-007261-b5fgb9wf	256	41	the	the	DT
cord-007261-b5fgb9wf	256	42	sugar	sugar	NN
cord-007261-b5fgb9wf	256	43	chain	chain	NN
cord-007261-b5fgb9wf	256	44	had	have	VBD
cord-007261-b5fgb9wf	256	45	not	not	RB
cord-007261-b5fgb9wf	256	46	been	be	VBN
cord-007261-b5fgb9wf	256	47	processed	process	VBN
cord-007261-b5fgb9wf	256	48	by	by	IN
cord-007261-b5fgb9wf	256	49	the	the	DT
cord-007261-b5fgb9wf	256	50	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	256	51	-	-	HYPH
cord-007261-b5fgb9wf	256	52	associated	associate	VBN
cord-007261-b5fgb9wf	256	53	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	256	54	.	.	.
cord-007261-b5fgb9wf	257	1	Their	-PRON-	PRP$
cord-007261-b5fgb9wf	257	2	observations	observation	NNS
cord-007261-b5fgb9wf	257	3	are	be	VBP
cord-007261-b5fgb9wf	257	4	consistent	consistent	JJ
cord-007261-b5fgb9wf	257	5	with	with	IN
cord-007261-b5fgb9wf	257	6	our	-PRON-	PRP$
cord-007261-b5fgb9wf	257	7	conclusion	conclusion	NN
cord-007261-b5fgb9wf	257	8	that	that	IN
cord-007261-b5fgb9wf	257	9	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	257	10	P450s	P450s	NNP
cord-007261-b5fgb9wf	257	11	are	be	VBP
cord-007261-b5fgb9wf	257	12	not	not	RB
cord-007261-b5fgb9wf	257	13	recycled	recycle	VBN
cord-007261-b5fgb9wf	257	14	between	between	IN
cord-007261-b5fgb9wf	257	15	the	the	DT
cord-007261-b5fgb9wf	257	16	ER	ER	NNP
cord-007261-b5fgb9wf	257	17	and	and	CC
cord-007261-b5fgb9wf	257	18	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	257	19	compartments	compartment	NNS
cord-007261-b5fgb9wf	257	20	.	.	.
cord-007261-b5fgb9wf	258	1	Thus	thus	RB
cord-007261-b5fgb9wf	258	2	,	,	,
cord-007261-b5fgb9wf	258	3	the	the	DT
cord-007261-b5fgb9wf	258	4	ER	ER	NNP
cord-007261-b5fgb9wf	258	5	residency	residency	NN
cord-007261-b5fgb9wf	258	6	of	of	IN
cord-007261-b5fgb9wf	258	7	P450s	P450s	NNP
cord-007261-b5fgb9wf	258	8	seems	seem	VBZ
cord-007261-b5fgb9wf	258	9	to	to	TO
cord-007261-b5fgb9wf	258	10	be	be	VB
cord-007261-b5fgb9wf	258	11	attained	attain	VBN
cord-007261-b5fgb9wf	258	12	through	through	IN
cord-007261-b5fgb9wf	258	13	a	a	DT
cord-007261-b5fgb9wf	258	14	unique	unique	JJ
cord-007261-b5fgb9wf	258	15	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	258	16	segment	segment	NN
cord-007261-b5fgb9wf	258	17	-	-	HYPH
cord-007261-b5fgb9wf	258	18	mediated	mediate	VBN
cord-007261-b5fgb9wf	258	19	mechanism	mechanism	NN
cord-007261-b5fgb9wf	258	20	,	,	,
cord-007261-b5fgb9wf	258	21	indicating	indicate	VBG
cord-007261-b5fgb9wf	258	22	the	the	DT
cord-007261-b5fgb9wf	258	23	functioning	functioning	NN
cord-007261-b5fgb9wf	258	24	of	of	IN
cord-007261-b5fgb9wf	258	25	the	the	DT
cord-007261-b5fgb9wf	258	26	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	258	27	segment	segment	NN
cord-007261-b5fgb9wf	258	28	as	as	IN
cord-007261-b5fgb9wf	258	29	the	the	DT
cord-007261-b5fgb9wf	258	30	ER	ER	NNP
cord-007261-b5fgb9wf	258	31	retention	retention	NN
cord-007261-b5fgb9wf	258	32	signal	signal	NN
cord-007261-b5fgb9wf	258	33	.	.	.
cord-007261-b5fgb9wf	259	1	There	there	EX
cord-007261-b5fgb9wf	259	2	have	have	VBP
cord-007261-b5fgb9wf	259	3	been	be	VBN
cord-007261-b5fgb9wf	259	4	several	several	JJ
cord-007261-b5fgb9wf	259	5	reports	report	NNS
cord-007261-b5fgb9wf	259	6	that	that	IN
cord-007261-b5fgb9wf	259	7	the	the	DT
cord-007261-b5fgb9wf	259	8	retention	retention	NN
cord-007261-b5fgb9wf	259	9	of	of	IN
cord-007261-b5fgb9wf	259	10	membrane	membrane	NN
cord-007261-b5fgb9wf	259	11	proteins	protein	NNS
cord-007261-b5fgb9wf	259	12	in	in	IN
cord-007261-b5fgb9wf	259	13	the	the	DT
cord-007261-b5fgb9wf	259	14	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	259	15	compartments	compartment	NNS
cord-007261-b5fgb9wf	259	16	is	be	VBZ
cord-007261-b5fgb9wf	259	17	dependent	dependent	JJ
cord-007261-b5fgb9wf	259	18	on	on	IN
cord-007261-b5fgb9wf	259	19	the	the	DT
cord-007261-b5fgb9wf	259	20	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	259	21	segments	segment	NNS
cord-007261-b5fgb9wf	259	22	(	(	-LRB-
cord-007261-b5fgb9wf	259	23	35	35	CD
cord-007261-b5fgb9wf	259	24	)	)	-RRB-
cord-007261-b5fgb9wf	259	25	(	(	-LRB-
cord-007261-b5fgb9wf	259	26	36	36	CD
cord-007261-b5fgb9wf	259	27	)	)	-RRB-
cord-007261-b5fgb9wf	259	28	(	(	-LRB-
cord-007261-b5fgb9wf	259	29	37	37	CD
cord-007261-b5fgb9wf	259	30	)	)	-RRB-
cord-007261-b5fgb9wf	259	31	(	(	-LRB-
cord-007261-b5fgb9wf	259	32	38	38	CD
cord-007261-b5fgb9wf	259	33	)	)	-RRB-
cord-007261-b5fgb9wf	259	34	(	(	-LRB-
cord-007261-b5fgb9wf	259	35	39	39	CD
cord-007261-b5fgb9wf	259	36	)	)	-RRB-
cord-007261-b5fgb9wf	259	37	and	and	CC
cord-007261-b5fgb9wf	259	38	the	the	DT
cord-007261-b5fgb9wf	259	39	charged	charge	VBN
cord-007261-b5fgb9wf	259	40	residues	residue	NNS
cord-007261-b5fgb9wf	259	41	flunking	flunk	VBG
cord-007261-b5fgb9wf	259	42	the	the	DT
cord-007261-b5fgb9wf	259	43	segments	segment	NNS
cord-007261-b5fgb9wf	259	44	(	(	-LRB-
cord-007261-b5fgb9wf	259	45	40	40	CD
cord-007261-b5fgb9wf	259	46	)	)	-RRB-
cord-007261-b5fgb9wf	259	47	(	(	-LRB-
cord-007261-b5fgb9wf	259	48	41	41	CD
cord-007261-b5fgb9wf	259	49	)	)	-RRB-
cord-007261-b5fgb9wf	259	50	(	(	-LRB-
cord-007261-b5fgb9wf	259	51	42	42	CD
cord-007261-b5fgb9wf	259	52	)	)	-RRB-
cord-007261-b5fgb9wf	259	53	.	.	.
cord-007261-b5fgb9wf	260	1	The	the	DT
cord-007261-b5fgb9wf	260	2	signal	signal	NN
cord-007261-b5fgb9wf	260	3	-	-	HYPH
cord-007261-b5fgb9wf	260	4	anchor	anchor	NN
cord-007261-b5fgb9wf	260	5	region	region	NN
cord-007261-b5fgb9wf	260	6	of	of	IN
cord-007261-b5fgb9wf	260	7	P450-(Ml	p450-(ml	$
cord-007261-b5fgb9wf	260	8	)	)	-RRB-
cord-007261-b5fgb9wf	260	9	has	have	VBZ
cord-007261-b5fgb9wf	260	10	no	no	DT
cord-007261-b5fgb9wf	260	11	conserved	conserve	VBN
cord-007261-b5fgb9wf	260	12	sequence	sequence	NN
cord-007261-b5fgb9wf	260	13	,	,	,
cord-007261-b5fgb9wf	260	14	as	as	IN
cord-007261-b5fgb9wf	260	15	compared	compare	VBN
cord-007261-b5fgb9wf	260	16	with	with	IN
cord-007261-b5fgb9wf	260	17	the	the	DT
cord-007261-b5fgb9wf	260	18	corresponding	correspond	VBG
cord-007261-b5fgb9wf	260	19	proteins	protein	NNS
cord-007261-b5fgb9wf	260	20	of	of	IN
cord-007261-b5fgb9wf	260	21	all	all	DT
cord-007261-b5fgb9wf	260	22	known	know	VBN
cord-007261-b5fgb9wf	260	23	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	261	1	P450s	p450s	UH
cord-007261-b5fgb9wf	261	2	.	.	.
cord-007261-b5fgb9wf	262	1	The	the	DT
cord-007261-b5fgb9wf	262	2	N	n	CD
cord-007261-b5fgb9wf	262	3	-	-	HYPH
cord-007261-b5fgb9wf	262	4	terminal	terminal	NN
cord-007261-b5fgb9wf	262	5	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	262	6	region	region	NN
cord-007261-b5fgb9wf	262	7	of	of	IN
cord-007261-b5fgb9wf	262	8	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	262	9	P-4508	P-4508	NNP
cord-007261-b5fgb9wf	262	10	and	and	CC
cord-007261-b5fgb9wf	262	11	the	the	DT
cord-007261-b5fgb9wf	262	12	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	262	13	region	region	NN
cord-007261-b5fgb9wf	262	14	of	of	IN
cord-007261-b5fgb9wf	262	15	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	262	16	membrane	membrane	NN
cord-007261-b5fgb9wf	262	17	proteins	protein	NNS
cord-007261-b5fgb9wf	262	18	comprise	comprise	VBP
cord-007261-b5fgb9wf	262	19	similar	similar	JJ
cord-007261-b5fgb9wf	262	20	hydrophobic	hydrophobic	JJ
cord-007261-b5fgb9wf	262	21	stretches	stretch	NNS
cord-007261-b5fgb9wf	262	22	,	,	,
cord-007261-b5fgb9wf	262	23	but	but	CC
cord-007261-b5fgb9wf	262	24	the	the	DT
cord-007261-b5fgb9wf	262	25	former	former	JJ
cord-007261-b5fgb9wf	262	26	retains	retain	VBZ
cord-007261-b5fgb9wf	262	27	the	the	DT
cord-007261-b5fgb9wf	262	28	proteins	protein	NNS
cord-007261-b5fgb9wf	262	29	in	in	IN
cord-007261-b5fgb9wf	262	30	the	the	DT
cord-007261-b5fgb9wf	262	31	ER	ER	NNP
cord-007261-b5fgb9wf	262	32	whereas	whereas	IN
cord-007261-b5fgb9wf	262	33	the	the	DT
cord-007261-b5fgb9wf	262	34	latter	latter	NN
cord-007261-b5fgb9wf	262	35	keeps	keep	VBZ
cord-007261-b5fgb9wf	262	36	the	the	DT
cord-007261-b5fgb9wf	262	37	proteins	protein	NNS
cord-007261-b5fgb9wf	262	38	in	in	IN
cord-007261-b5fgb9wf	262	39	the	the	DT
cord-007261-b5fgb9wf	262	40	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	262	41	membrane	membrane	NN
cord-007261-b5fgb9wf	262	42	.	.	.
cord-007261-b5fgb9wf	263	1	These	these	DT
cord-007261-b5fgb9wf	263	2	two	two	CD
cord-007261-b5fgb9wf	263	3	types	type	NNS
cord-007261-b5fgb9wf	263	4	of	of	IN
cord-007261-b5fgb9wf	263	5	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	263	6	segments	segment	NNS
cord-007261-b5fgb9wf	263	7	must	must	MD
cord-007261-b5fgb9wf	263	8	contain	contain	VB
cord-007261-b5fgb9wf	263	9	unique	unique	JJ
cord-007261-b5fgb9wf	263	10	information	information	NN
cord-007261-b5fgb9wf	263	11	for	for	IN
cord-007261-b5fgb9wf	263	12	the	the	DT
cord-007261-b5fgb9wf	263	13	retention	retention	NN
cord-007261-b5fgb9wf	263	14	of	of	IN
cord-007261-b5fgb9wf	263	15	membrane	membrane	NN
cord-007261-b5fgb9wf	263	16	proteins	protein	NNS
cord-007261-b5fgb9wf	263	17	in	in	IN
cord-007261-b5fgb9wf	263	18	different	different	JJ
cord-007261-b5fgb9wf	263	19	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	263	20	membranes	membrane	NNS
cord-007261-b5fgb9wf	263	21	,	,	,
cord-007261-b5fgb9wf	263	22	and	and	CC
cord-007261-b5fgb9wf	263	23	do	do	VBP
cord-007261-b5fgb9wf	263	24	not	not	RB
cord-007261-b5fgb9wf	263	25	show	show	VB
cord-007261-b5fgb9wf	263	26	any	any	DT
cord-007261-b5fgb9wf	263	27	homology	homology	NN
cord-007261-b5fgb9wf	263	28	to	to	IN
cord-007261-b5fgb9wf	263	29	each	each	DT
cord-007261-b5fgb9wf	263	30	other	other	JJ
cord-007261-b5fgb9wf	263	31	.	.	.
cord-007261-b5fgb9wf	264	1	The	the	DT
cord-007261-b5fgb9wf	264	2	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	264	3	region	region	NN
cord-007261-b5fgb9wf	264	4	of	of	IN
cord-007261-b5fgb9wf	264	5	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	264	6	P450s	P450s	NNP
cord-007261-b5fgb9wf	264	7	may	may	MD
cord-007261-b5fgb9wf	264	8	associate	associate	VB
cord-007261-b5fgb9wf	264	9	together	together	RB
cord-007261-b5fgb9wf	264	10	in	in	IN
cord-007261-b5fgb9wf	264	11	the	the	DT
cord-007261-b5fgb9wf	264	12	membrane	membrane	NN
cord-007261-b5fgb9wf	264	13	to	to	TO
cord-007261-b5fgb9wf	264	14	form	form	VB
cord-007261-b5fgb9wf	264	15	homo	homo	NN
cord-007261-b5fgb9wf	264	16	-	-	HYPH
cord-007261-b5fgb9wf	264	17	oligomers	oligomer	NNS
cord-007261-b5fgb9wf	264	18	or	or	CC
cord-007261-b5fgb9wf	264	19	interact	interact	VB
cord-007261-b5fgb9wf	264	20	with	with	IN
cord-007261-b5fgb9wf	264	21	other	other	JJ
cord-007261-b5fgb9wf	264	22	membrane	membrane	NN
cord-007261-b5fgb9wf	264	23	proteins	protein	NNS
cord-007261-b5fgb9wf	264	24	to	to	TO
cord-007261-b5fgb9wf	264	25	assemble	assemble	VB
cord-007261-b5fgb9wf	264	26	into	into	IN
cord-007261-b5fgb9wf	264	27	a	a	DT
cord-007261-b5fgb9wf	264	28	network	network	NN
cord-007261-b5fgb9wf	264	29	of	of	IN
cord-007261-b5fgb9wf	264	30	membrane	membrane	NN
cord-007261-b5fgb9wf	264	31	proteins	protein	NNS
cord-007261-b5fgb9wf	264	32	,	,	,
cord-007261-b5fgb9wf	264	33	which	which	WDT
cord-007261-b5fgb9wf	264	34	can	can	MD
cord-007261-b5fgb9wf	264	35	not	not	RB
cord-007261-b5fgb9wf	264	36	enter	enter	VB
cord-007261-b5fgb9wf	264	37	the	the	DT
cord-007261-b5fgb9wf	264	38	transport	transport	NN
cord-007261-b5fgb9wf	264	39	vesicles	vesicle	NNS
cord-007261-b5fgb9wf	264	40	exiting	exit	VBG
cord-007261-b5fgb9wf	264	41	from	from	IN
cord-007261-b5fgb9wf	264	42	the	the	DT
cord-007261-b5fgb9wf	264	43	ER	ER	NNP
cord-007261-b5fgb9wf	264	44	.	.	.
cord-007261-b5fgb9wf	265	1	The	the	DT
cord-007261-b5fgb9wf	265	2	function	function	NN
cord-007261-b5fgb9wf	265	3	of	of	IN
cord-007261-b5fgb9wf	265	4	the	the	DT
cord-007261-b5fgb9wf	265	5	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	265	6	region	region	NN
cord-007261-b5fgb9wf	265	7	in	in	IN
cord-007261-b5fgb9wf	265	8	the	the	DT
cord-007261-b5fgb9wf	265	9	ER	ER	NNP
cord-007261-b5fgb9wf	265	10	retention	retention	NN
cord-007261-b5fgb9wf	265	11	of	of	IN
cord-007261-b5fgb9wf	265	12	P450s	P450s	NNP
cord-007261-b5fgb9wf	265	13	has	have	VBZ
cord-007261-b5fgb9wf	265	14	to	to	TO
cord-007261-b5fgb9wf	265	15	be	be	VB
cord-007261-b5fgb9wf	265	16	clarified	clarify	VBN
cord-007261-b5fgb9wf	265	17	in	in	IN
cord-007261-b5fgb9wf	265	18	the	the	DT
cord-007261-b5fgb9wf	265	19	future	future	NN
cord-007261-b5fgb9wf	265	20	.	.	.
cord-007261-b5fgb9wf	266	1	After	after	IN
cord-007261-b5fgb9wf	266	2	completion	completion	NN
cord-007261-b5fgb9wf	266	3	of	of	IN
cord-007261-b5fgb9wf	266	4	this	this	DT
cord-007261-b5fgb9wf	266	5	study	study	NN
cord-007261-b5fgb9wf	266	6	,	,	,
cord-007261-b5fgb9wf	266	7	Ann	Ann	NNP
cord-007261-b5fgb9wf	266	8	et	et	NNP
cord-007261-b5fgb9wf	266	9	al	al	NNP
cord-007261-b5fgb9wf	266	10	.	.	.
cord-007261-b5fgb9wf	267	1	(	(	-LRB-
cord-007261-b5fgb9wf	267	2	43	43	CD
cord-007261-b5fgb9wf	267	3	)	)	-RRB-
cord-007261-b5fgb9wf	267	4	reported	report	VBD
cord-007261-b5fgb9wf	267	5	that	that	IN
cord-007261-b5fgb9wf	267	6	the	the	DT
cord-007261-b5fgb9wf	267	7	amino	amino	NN
cord-007261-b5fgb9wf	267	8	-	-	HYPH
cord-007261-b5fgb9wf	267	9	terminal	terminal	NN
cord-007261-b5fgb9wf	267	10	29	29	CD
cord-007261-b5fgb9wf	267	11	amino	amino	JJ
cord-007261-b5fgb9wf	267	12	acids	acid	NNS
cord-007261-b5fgb9wf	267	13	of	of	IN
cord-007261-b5fgb9wf	267	14	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	267	15	P450	p450	NN
cord-007261-b5fgb9wf	267	16	(	(	-LRB-
cord-007261-b5fgb9wf	267	17	2C1	2c1	CD
cord-007261-b5fgb9wf	267	18	)	)	-RRB-
cord-007261-b5fgb9wf	267	19	are	be	VBP
cord-007261-b5fgb9wf	267	20	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	267	21	for	for	IN
cord-007261-b5fgb9wf	267	22	its	-PRON-	PRP$
cord-007261-b5fgb9wf	267	23	retention	retention	NN
cord-007261-b5fgb9wf	267	24	in	in	IN
cord-007261-b5fgb9wf	267	25	the	the	DT
cord-007261-b5fgb9wf	267	26	ER	ER	NNP
cord-007261-b5fgb9wf	267	27	.	.	.
cord-007261-b5fgb9wf	268	1	Biosynthetic	biosynthetic	JJ
cord-007261-b5fgb9wf	268	2	protein	protein	NN
cord-007261-b5fgb9wf	268	3	transport	transport	NN
cord-007261-b5fgb9wf	268	4	and	and	CC
cord-007261-b5fgb9wf	268	5	sorting	sorting	NN
cord-007261-b5fgb9wf	268	6	by	by	IN
cord-007261-b5fgb9wf	268	7	the	the	DT
cord-007261-b5fgb9wf	268	8	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	268	9	reticulum	reticulum	NN
cord-007261-b5fgb9wf	268	10	and	and	CC
cord-007261-b5fgb9wf	268	11	Golgi	golgi	NN
cord-007261-b5fgb9wf	269	1	The	the	DT
cord-007261-b5fgb9wf	269	2	rate	rate	NN
cord-007261-b5fgb9wf	269	3	of	of	IN
cord-007261-b5fgb9wf	269	4	bulk	bulk	JJ
cord-007261-b5fgb9wf	269	5	flow	flow	NN
cord-007261-b5fgb9wf	269	6	from	from	IN
cord-007261-b5fgb9wf	269	7	the	the	DT
cord-007261-b5fgb9wf	269	8	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	269	9	reticulum	reticulum	NN
cord-007261-b5fgb9wf	269	10	to	to	IN
cord-007261-b5fgb9wf	269	11	the	the	DT
cord-007261-b5fgb9wf	269	12	cell	cell	NN
cord-007261-b5fgb9wf	269	13	surface	surface	NN
cord-007261-b5fgb9wf	269	14	Assembly	Assembly	NNP
cord-007261-b5fgb9wf	269	15	of	of	IN
cord-007261-b5fgb9wf	269	16	asparaginelinked	asparaginelinke	VBN
cord-007261-b5fgb9wf	269	17	oligosaccharides	oligosaccharide	NNS
cord-007261-b5fgb9wf	270	1	Lysosomal	Lysosomal	NNP
cord-007261-b5fgb9wf	270	2	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	270	3	and	and	CC
cord-007261-b5fgb9wf	270	4	their	-PRON-	PRP$
cord-007261-b5fgb9wf	270	5	receptors	receptor	NNS
cord-007261-b5fgb9wf	271	1	A	a	DT
cord-007261-b5fgb9wf	271	2	C	c	NN
cord-007261-b5fgb9wf	271	3	-	-	HYPH
cord-007261-b5fgb9wf	271	4	terminal	terminal	NN
cord-007261-b5fgb9wf	271	5	signal	signal	NN
cord-007261-b5fgb9wf	271	6	prevents	prevent	VBZ
cord-007261-b5fgb9wf	271	7	secretion	secretion	NN
cord-007261-b5fgb9wf	271	8	of	of	IN
cord-007261-b5fgb9wf	271	9	luminal	luminal	JJ
cord-007261-b5fgb9wf	271	10	ER	er	NN
cord-007261-b5fgb9wf	271	11	proteins	protein	NNS
cord-007261-b5fgb9wf	271	12	Evidence	evidence	NN
cord-007261-b5fgb9wf	271	13	that	that	IN
cord-007261-b5fgb9wf	271	14	luminal	luminal	JJ
cord-007261-b5fgb9wf	271	15	ER	er	NN
cord-007261-b5fgb9wf	271	16	proteins	protein	NNS
cord-007261-b5fgb9wf	271	17	are	be	VBP
cord-007261-b5fgb9wf	271	18	sorted	sort	VBN
cord-007261-b5fgb9wf	271	19	from	from	IN
cord-007261-b5fgb9wf	271	20	secreted	secreted	JJ
cord-007261-b5fgb9wf	271	21	proteins	protein	NNS
cord-007261-b5fgb9wf	271	22	in	in	IN
cord-007261-b5fgb9wf	271	23	a	a	DT
cord-007261-b5fgb9wf	271	24	poat	poat	NNP
cord-007261-b5fgb9wf	271	25	-	-	HYPH
cord-007261-b5fgb9wf	271	26	ER	ER	NNP
cord-007261-b5fgb9wf	271	27	compartment	compartment	NN
cord-007261-b5fgb9wf	271	28	Identification	identification	NN
cord-007261-b5fgb9wf	271	29	of	of	IN
cord-007261-b5fgb9wf	271	30	a	a	DT
cord-007261-b5fgb9wf	271	31	consensus	consensus	NN
cord-007261-b5fgb9wf	271	32	motif	motif	NN
cord-007261-b5fgb9wf	271	33	for	for	IN
cord-007261-b5fgb9wf	271	34	retention	retention	NN
cord-007261-b5fgb9wf	271	35	of	of	IN
cord-007261-b5fgb9wf	271	36	transmembrane	transmembrane	JJ
cord-007261-b5fgb9wf	271	37	proteins	protein	NNS
cord-007261-b5fgb9wf	271	38	in	in	IN
cord-007261-b5fgb9wf	271	39	the	the	DT
cord-007261-b5fgb9wf	271	40	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	271	41	reticulum	reticulum	NN
cord-007261-b5fgb9wf	271	42	Retrieval	Retrieval	NNP
cord-007261-b5fgb9wf	271	43	of	of	IN
cord-007261-b5fgb9wf	271	44	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	271	45	proteins	protein	NNS
cord-007261-b5fgb9wf	271	46	to	to	IN
cord-007261-b5fgb9wf	271	47	the	the	DT
cord-007261-b5fgb9wf	271	48	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	271	49	reticulum	reticulum	NN
cord-007261-b5fgb9wf	271	50	Signals	signal	NNS
cord-007261-b5fgb9wf	271	51	for	for	IN
cord-007261-b5fgb9wf	271	52	the	the	DT
cord-007261-b5fgb9wf	271	53	incorporation	incorporation	NN
cord-007261-b5fgb9wf	271	54	and	and	CC
cord-007261-b5fgb9wf	271	55	orientation	orientation	NN
cord-007261-b5fgb9wf	271	56	of	of	IN
cord-007261-b5fgb9wf	271	57	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	271	58	P450	p450	NN
cord-007261-b5fgb9wf	271	59	in	in	IN
cord-007261-b5fgb9wf	271	60	the	the	DT
cord-007261-b5fgb9wf	271	61	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	271	62	reticulum	reticulum	NN
cord-007261-b5fgb9wf	271	63	membrane	membrane	NN
cord-007261-b5fgb9wf	271	64	Positive	positive	JJ
cord-007261-b5fgb9wf	271	65	charges	charge	NNS
cord-007261-b5fgb9wf	271	66	at	at	IN
cord-007261-b5fgb9wf	271	67	the	the	DT
cord-007261-b5fgb9wf	271	68	NH	NH	NNP
cord-007261-b5fgb9wf	271	69	,	,	,
cord-007261-b5fgb9wf	271	70	terminus	terminus	NN
cord-007261-b5fgb9wf	271	71	convert	convert	VBP
cord-007261-b5fgb9wf	271	72	the	the	DT
cord-007261-b5fgb9wf	271	73	membrane	membrane	NN
cord-007261-b5fgb9wf	271	74	-	-	HYPH
cord-007261-b5fgb9wf	271	75	anchor	anchor	NN
cord-007261-b5fgb9wf	271	76	signal	signal	NN
cord-007261-b5fgb9wf	271	77	peptide	peptide	NN
cord-007261-b5fgb9wf	271	78	of	of	IN
cord-007261-b5fgb9wf	271	79	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	271	80	P-450	p-450	NN
cord-007261-b5fgb9wf	271	81	to	to	IN
cord-007261-b5fgb9wf	271	82	a	a	DT
cord-007261-b5fgb9wf	271	83	secretory	secretory	JJ
cord-007261-b5fgb9wf	271	84	signal	signal	NN
cord-007261-b5fgb9wf	271	85	peptide	peptide	NN
cord-007261-b5fgb9wf	271	86	A	a	DT
cord-007261-b5fgb9wf	271	87	short	short	JJ
cord-007261-b5fgb9wf	271	88	aminoterminal	aminoterminal	JJ
cord-007261-b5fgb9wf	271	89	segment	segment	NN
cord-007261-b5fgb9wf	271	90	of	of	IN
cord-007261-b5fgb9wf	271	91	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	271	92	cytochrome	cytochrome	NNP
cord-007261-b5fgb9wf	271	93	P-450	P-450	VBD
cord-007261-b5fgb9wf	271	94	functions	function	VBZ
cord-007261-b5fgb9wf	271	95	both	both	DT
cord-007261-b5fgb9wf	271	96	as	as	IN
cord-007261-b5fgb9wf	271	97	an	an	DT
cord-007261-b5fgb9wf	271	98	insertion	insertion	NN
cord-007261-b5fgb9wf	271	99	signal	signal	NN
cord-007261-b5fgb9wf	271	100	and	and	CC
cord-007261-b5fgb9wf	271	101	as	as	IN
cord-007261-b5fgb9wf	271	102	a	a	DT
cord-007261-b5fgb9wf	271	103	stop	stop	VB
cord-007261-b5fgb9wf	271	104	-	-	HYPH
cord-007261-b5fgb9wf	271	105	transfer	transfer	NN
cord-007261-b5fgb9wf	271	106	sequence	sequence	NN
cord-007261-b5fgb9wf	271	107	Core	core	NN
cord-007261-b5fgb9wf	271	108	glycosylation	glycosylation	NN
cord-007261-b5fgb9wf	271	109	of	of	IN
cord-007261-b5fgb9wf	271	110	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	271	111	P-450(arom	P-450(arom	NNP
cord-007261-b5fgb9wf	271	112	)	)	-RRB-
cord-007261-b5fgb9wf	272	1	Molecular	molecular	JJ
cord-007261-b5fgb9wf	272	2	cloning	cloning	NN
cord-007261-b5fgb9wf	272	3	and	and	CC
cord-007261-b5fgb9wf	272	4	nucleotide	nucleotide	JJ
cord-007261-b5fgb9wf	272	5	sequence	sequence	NN
cord-007261-b5fgb9wf	272	6	of	of	IN
cord-007261-b5fgb9wf	272	7	cDNA	cDNA	NNP
cord-007261-b5fgb9wf	272	8	of	of	IN
cord-007261-b5fgb9wf	272	9	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	272	10	carboxyesterase	carboxyesterase	NNP
cord-007261-b5fgb9wf	272	11	El	El	NNP
cord-007261-b5fgb9wf	272	12	of	of	IN
cord-007261-b5fgb9wf	272	13	rat	rat	NNP
cord-007261-b5fgb9wf	272	14	liver	liver	NN
cord-007261-b5fgb9wf	272	15	Expression	expression	NN
cord-007261-b5fgb9wf	272	16	of	of	IN
cord-007261-b5fgb9wf	272	17	a	a	DT
cord-007261-b5fgb9wf	272	18	rat	rat	NN
cord-007261-b5fgb9wf	272	19	liver	liver	NN
cord-007261-b5fgb9wf	272	20	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	272	21	cytochrome	cytochrome	NNP
cord-007261-b5fgb9wf	272	22	P-450	P-450	VBD
cord-007261-b5fgb9wf	272	23	catalyzing	catalyze	VBG
cord-007261-b5fgb9wf	272	24	testosterone	testosterone	NN
cord-007261-b5fgb9wf	272	25	16or	16or	NN
cord-007261-b5fgb9wf	272	26	-	-	HYPH
cord-007261-b5fgb9wf	272	27	hydroxylation	hydroxylation	NN
cord-007261-b5fgb9wf	272	28	in	in	IN
cord-007261-b5fgb9wf	272	29	Saccharomyces	Saccharomyces	NNP
cord-007261-b5fgb9wf	272	30	cerevisiae	cerevisiae	NNS
cord-007261-b5fgb9wf	272	31	:	:	:
cord-007261-b5fgb9wf	273	1	Vitamin	vitamin	NN
cord-007261-b5fgb9wf	273	2	D	d	NN
cord-007261-b5fgb9wf	273	3	3	3	CD
cord-007261-b5fgb9wf	273	4	25-hydroxylase	25-hydroxylase	CD
cord-007261-b5fgb9wf	273	5	and	and	CC
cord-007261-b5fgb9wf	273	6	testosterone	testosterone	NN
cord-007261-b5fgb9wf	273	7	16o--	16o--	CD
cord-007261-b5fgb9wf	274	1	hydroxylase	hydroxylase	NNP
cord-007261-b5fgb9wf	274	2	are	be	VBP
cord-007261-b5fgb9wf	274	3	distinct	distinct	JJ
cord-007261-b5fgb9wf	274	4	forms	form	NNS
cord-007261-b5fgb9wf	274	5	of	of	IN
cord-007261-b5fgb9wf	274	6	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	274	7	P-450	p-450	NN
cord-007261-b5fgb9wf	275	1	Importance	importance	NN
cord-007261-b5fgb9wf	275	2	of	of	IN
cord-007261-b5fgb9wf	275	3	the	the	DT
cord-007261-b5fgb9wf	275	4	proline	proline	JJ
cord-007261-b5fgb9wf	275	5	-	-	HYPH
cord-007261-b5fgb9wf	275	6	rich	rich	JJ
cord-007261-b5fgb9wf	275	7	region	region	NN
cord-007261-b5fgb9wf	275	8	following	follow	VBG
cord-007261-b5fgb9wf	275	9	signal	signal	NN
cord-007261-b5fgb9wf	275	10	-	-	HYPH
cord-007261-b5fgb9wf	275	11	anchor	anchor	NN
cord-007261-b5fgb9wf	275	12	sequence	sequence	NN
cord-007261-b5fgb9wf	275	13	in	in	IN
cord-007261-b5fgb9wf	275	14	the	the	DT
cord-007261-b5fgb9wf	275	15	formation	formation	NN
cord-007261-b5fgb9wf	275	16	of	of	IN
cord-007261-b5fgb9wf	275	17	correct	correct	JJ
cord-007261-b5fgb9wf	275	18	conformation	conformation	NN
cord-007261-b5fgb9wf	275	19	of	of	IN
cord-007261-b5fgb9wf	275	20	microsomal	microsomal	NNP
cord-007261-b5fgb9wf	275	21	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	275	22	P-450s	P-450s	NNP
cord-007261-b5fgb9wf	275	23	Purification	Purification	NNP
cord-007261-b5fgb9wf	275	24	and	and	CC
cord-007261-b5fgb9wf	275	25	characterization	characterization	NN
cord-007261-b5fgb9wf	275	26	of	of	IN
cord-007261-b5fgb9wf	275	27	three	three	CD
cord-007261-b5fgb9wf	275	28	male	male	NN
cord-007261-b5fgb9wf	275	29	-	-	HYPH
cord-007261-b5fgb9wf	275	30	specific	specific	JJ
cord-007261-b5fgb9wf	275	31	and	and	CC
cord-007261-b5fgb9wf	275	32	one	one	CD
cord-007261-b5fgb9wf	275	33	female	female	JJ
cord-007261-b5fgb9wf	275	34	-	-	HYPH
cord-007261-b5fgb9wf	275	35	specific	specific	JJ
cord-007261-b5fgb9wf	275	36	forms	form	NNS
cord-007261-b5fgb9wf	275	37	of	of	IN
cord-007261-b5fgb9wf	275	38	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	276	1	P-450	P-450	VBN
cord-007261-b5fgb9wf	276	2	from	from	IN
cord-007261-b5fgb9wf	276	3	rat	rat	NN
cord-007261-b5fgb9wf	276	4	liver	liver	NN
cord-007261-b5fgb9wf	276	5	microsomes	microsomes	NNP
cord-007261-b5fgb9wf	276	6	Mitochondrial	Mitochondrial	NNP
cord-007261-b5fgb9wf	276	7	targeting	target	VBG
cord-007261-b5fgb9wf	276	8	signal	signal	NN
cord-007261-b5fgb9wf	276	9	of	of	IN
cord-007261-b5fgb9wf	276	10	rat	rat	NN
cord-007261-b5fgb9wf	276	11	liver	liver	NN
cord-007261-b5fgb9wf	276	12	monoamine	monoamine	NN
cord-007261-b5fgb9wf	276	13	oxidase	oxidase	NN
cord-007261-b5fgb9wf	276	14	B	b	NN
cord-007261-b5fgb9wf	276	15	is	be	VBZ
cord-007261-b5fgb9wf	276	16	located	locate	VBN
cord-007261-b5fgb9wf	276	17	at	at	IN
cord-007261-b5fgb9wf	276	18	its	-PRON-	PRP$
cord-007261-b5fgb9wf	276	19	carboxy	carboxy	JJ
cord-007261-b5fgb9wf	276	20	terminus	terminus	NN
cord-007261-b5fgb9wf	276	21	An	an	DT
cord-007261-b5fgb9wf	276	22	hsp70-like	hsp70-like	JJ
cord-007261-b5fgb9wf	276	23	protein	protein	NN
cord-007261-b5fgb9wf	276	24	in	in	IN
cord-007261-b5fgb9wf	276	25	the	the	DT
cord-007261-b5fgb9wf	276	26	ER	er	NN
cord-007261-b5fgb9wf	276	27	:	:	:
cord-007261-b5fgb9wf	276	28	Identity	identity	NN
cord-007261-b5fgb9wf	276	29	with	with	IN
cord-007261-b5fgb9wf	276	30	the	the	DT
cord-007261-b5fgb9wf	276	31	78	78	CD
cord-007261-b5fgb9wf	276	32	kd	kd	NNP
cord-007261-b5fgb9wf	276	33	glucose	glucose	NN
cord-007261-b5fgb9wf	276	34	-	-	HYPH
cord-007261-b5fgb9wf	276	35	regulated	regulate	VBN
cord-007261-b5fgb9wf	276	36	protein	protein	NN
cord-007261-b5fgb9wf	276	37	and	and	CC
cord-007261-b5fgb9wf	276	38	immunoglobulin	immunoglobulin	NN
cord-007261-b5fgb9wf	276	39	heavy	heavy	JJ
cord-007261-b5fgb9wf	276	40	chain	chain	NN
cord-007261-b5fgb9wf	276	41	binding	bind	VBG
cord-007261-b5fgb9wf	276	42	protein	protein	NN
cord-007261-b5fgb9wf	276	43	Posttranslational	Posttranslational	NNP
cord-007261-b5fgb9wf	276	44	association	association	NNP
cord-007261-b5fgb9wf	276	45	of	of	IN
cord-007261-b5fgb9wf	276	46	immunoglobulin	immunoglobulin	NNP
cord-007261-b5fgb9wf	276	47	heavy	heavy	JJ
cord-007261-b5fgb9wf	276	48	chain	chain	NN
cord-007261-b5fgb9wf	276	49	binding	bind	VBG
cord-007261-b5fgb9wf	276	50	protein	protein	NN
cord-007261-b5fgb9wf	276	51	with	with	IN
cord-007261-b5fgb9wf	276	52	nascent	nascent	JJ
cord-007261-b5fgb9wf	276	53	heavy	heavy	JJ
cord-007261-b5fgb9wf	276	54	chains	chain	NNS
cord-007261-b5fgb9wf	276	55	in	in	IN
cord-007261-b5fgb9wf	276	56	nonsecreting	nonsecreting	NN
cord-007261-b5fgb9wf	276	57	and	and	CC
cord-007261-b5fgb9wf	276	58	secreting	secrete	VBG
cord-007261-b5fgb9wf	276	59	hybridomas	hybridomas	NN
cord-007261-b5fgb9wf	276	60	Isolation	isolation	NN
cord-007261-b5fgb9wf	276	61	of	of	IN
cord-007261-b5fgb9wf	276	62	intracellular	intracellular	JJ
cord-007261-b5fgb9wf	276	63	membranes	membrane	NNS
cord-007261-b5fgb9wf	276	64	by	by	IN
cord-007261-b5fgb9wf	276	65	means	mean	NNS
cord-007261-b5fgb9wf	276	66	of	of	IN
cord-007261-b5fgb9wf	276	67	sodium	sodium	NN
cord-007261-b5fgb9wf	276	68	carbonate	carbonate	NN
cord-007261-b5fgb9wf	276	69	treatment	treatment	NN
cord-007261-b5fgb9wf	276	70	:	:	:
cord-007261-b5fgb9wf	276	71	Application	application	NN
cord-007261-b5fgb9wf	276	72	to	to	TO
cord-007261-b5fgb9wf	276	73	endoplasmic	endoplasmic	VB
cord-007261-b5fgb9wf	276	74	reticulum	reticulum	NN
cord-007261-b5fgb9wf	276	75	Electrophoretic	Electrophoretic	NNP
cord-007261-b5fgb9wf	276	76	transfer	transfer	NN
cord-007261-b5fgb9wf	276	77	of	of	IN
cord-007261-b5fgb9wf	276	78	proteins	protein	NNS
cord-007261-b5fgb9wf	276	79	from	from	IN
cord-007261-b5fgb9wf	276	80	polyacrylamide	polyacrylamide	JJ
cord-007261-b5fgb9wf	276	81	gels	gel	NNS
cord-007261-b5fgb9wf	276	82	to	to	IN
cord-007261-b5fgb9wf	276	83	nitrocellulose	nitrocellulose	NN
cord-007261-b5fgb9wf	276	84	sheets	sheet	NNS
cord-007261-b5fgb9wf	276	85	:	:	:
cord-007261-b5fgb9wf	276	86	Procedure	procedure	NN
cord-007261-b5fgb9wf	276	87	and	and	CC
cord-007261-b5fgb9wf	276	88	some	some	DT
cord-007261-b5fgb9wf	276	89	applications	application	NNS
cord-007261-b5fgb9wf	276	90	Reaction	Reaction	NNP
cord-007261-b5fgb9wf	276	91	of	of	IN
cord-007261-b5fgb9wf	276	92	a	a	DT
cord-007261-b5fgb9wf	276	93	microsomal	microsomal	JJ
cord-007261-b5fgb9wf	276	94	esterase	esterase	NN
cord-007261-b5fgb9wf	276	95	from	from	IN
cord-007261-b5fgb9wf	276	96	hog	hog	NN
cord-007261-b5fgb9wf	276	97	-	-	HYPH
cord-007261-b5fgb9wf	276	98	liver	liver	NN
cord-007261-b5fgb9wf	276	99	with	with	IN
cord-007261-b5fgb9wf	276	100	diethyl	diethyl	NNP
cord-007261-b5fgb9wf	277	1	p	p	LS
cord-007261-b5fgb9wf	277	2	-	-	HYPH
cord-007261-b5fgb9wf	277	3	nitrophenyl	nitrophenyl	NN
cord-007261-b5fgb9wf	277	4	phosphate	phosphate	NN
cord-007261-b5fgb9wf	277	5	A	a	DT
cord-007261-b5fgb9wf	277	6	new	new	JJ
cord-007261-b5fgb9wf	277	7	method	method	NN
cord-007261-b5fgb9wf	277	8	for	for	IN
cord-007261-b5fgb9wf	277	9	simultaneous	simultaneous	JJ
cord-007261-b5fgb9wf	277	10	purification	purification	NN
cord-007261-b5fgb9wf	277	11	of	of	IN
cord-007261-b5fgb9wf	277	12	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	277	13	IH	IH	NNP
cord-007261-b5fgb9wf	277	14	and	and	CC
cord-007261-b5fgb9wf	277	15	NADPH	NADPH	NNP
cord-007261-b5fgb9wf	277	16	-	-	HYPH
cord-007261-b5fgb9wf	277	17	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	277	18	c	c	NN
cord-007261-b5fgb9wf	277	19	reductase	reductase	NN
cord-007261-b5fgb9wf	277	20	from	from	IN
cord-007261-b5fgb9wf	277	21	rat	rat	NN
cord-007261-b5fgb9wf	277	22	liver	liver	NN
cord-007261-b5fgb9wf	277	23	microsomes	microsome	NNS
cord-007261-b5fgb9wf	277	24	Isolation	isolation	NN
cord-007261-b5fgb9wf	277	25	of	of	IN
cord-007261-b5fgb9wf	277	26	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	277	27	organelles	organelle	NNS
cord-007261-b5fgb9wf	278	1	A	a	DT
cord-007261-b5fgb9wf	278	2	soluble	soluble	JJ
cord-007261-b5fgb9wf	278	3	secretory	secretory	JJ
cord-007261-b5fgb9wf	278	4	protein	protein	NN
cord-007261-b5fgb9wf	278	5	is	be	VBZ
cord-007261-b5fgb9wf	278	6	first	first	RB
cord-007261-b5fgb9wf	278	7	concentrated	concentrate	VBN
cord-007261-b5fgb9wf	278	8	in	in	IN
cord-007261-b5fgb9wf	278	9	the	the	DT
cord-007261-b5fgb9wf	278	10	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	278	11	reticulum	reticulum	NN
cord-007261-b5fgb9wf	278	12	before	before	IN
cord-007261-b5fgb9wf	278	13	transfer	transfer	NN
cord-007261-b5fgb9wf	278	14	to	to	IN
cord-007261-b5fgb9wf	278	15	the	the	DT
cord-007261-b5fgb9wf	278	16	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	278	17	apparatus	apparatus	NN
cord-007261-b5fgb9wf	278	18	Subcellular	Subcellular	NNP
cord-007261-b5fgb9wf	278	19	location	location	NN
cord-007261-b5fgb9wf	278	20	of	of	IN
cord-007261-b5fgb9wf	278	21	two	two	CD
cord-007261-b5fgb9wf	278	22	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	278	23	involved	involve	VBN
cord-007261-b5fgb9wf	278	24	in	in	IN
cord-007261-b5fgb9wf	278	25	the	the	DT
cord-007261-b5fgb9wf	278	26	synthesis	synthesis	NN
cord-007261-b5fgb9wf	278	27	of	of	IN
cord-007261-b5fgb9wf	278	28	phosphorylated	phosphorylated	JJ
cord-007261-b5fgb9wf	278	29	recognition	recognition	NN
cord-007261-b5fgb9wf	278	30	markers	marker	NNS
cord-007261-b5fgb9wf	278	31	in	in	IN
cord-007261-b5fgb9wf	278	32	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	278	33	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	278	34	Synthesis	Synthesis	NNP
cord-007261-b5fgb9wf	278	35	of	of	IN
cord-007261-b5fgb9wf	278	36	phosphorylated	phosphorylated	JJ
cord-007261-b5fgb9wf	278	37	recognition	recognition	NN
cord-007261-b5fgb9wf	278	38	marker	marker	NN
cord-007261-b5fgb9wf	278	39	in	in	IN
cord-007261-b5fgb9wf	278	40	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	278	41	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	278	42	is	be	VBZ
cord-007261-b5fgb9wf	278	43	located	locate	VBN
cord-007261-b5fgb9wf	278	44	in	in	IN
cord-007261-b5fgb9wf	278	45	the	the	DT
cord-007261-b5fgb9wf	278	46	cis	cis	NNP
cord-007261-b5fgb9wf	278	47	part	part	NN
cord-007261-b5fgb9wf	278	48	of	of	IN
cord-007261-b5fgb9wf	278	49	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	278	50	apparatus	apparatus	NN
cord-007261-b5fgb9wf	278	51	Evidence	evidence	NN
cord-007261-b5fgb9wf	278	52	for	for	IN
cord-007261-b5fgb9wf	278	53	extensive	extensive	JJ
cord-007261-b5fgb9wf	278	54	subcellular	subcellular	JJ
cord-007261-b5fgb9wf	278	55	organization	organization	NN
cord-007261-b5fgb9wf	278	56	of	of	IN
cord-007261-b5fgb9wf	278	57	asparagine	asparagine	NN
cord-007261-b5fgb9wf	278	58	-	-	HYPH
cord-007261-b5fgb9wf	278	59	linked	link	VBN
cord-007261-b5fgb9wf	278	60	oligosaccharide	oligosaccharide	NN
cord-007261-b5fgb9wf	278	61	processing	processing	NN
cord-007261-b5fgb9wf	278	62	and	and	CC
cord-007261-b5fgb9wf	278	63	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	278	64	enzyme	enzyme	NN
cord-007261-b5fgb9wf	279	1	phosphorylation	phosphorylation	NN
cord-007261-b5fgb9wf	279	2	Subfractionation	subfractionation	NN
cord-007261-b5fgb9wf	279	3	of	of	IN
cord-007261-b5fgb9wf	279	4	rat	rat	NN
cord-007261-b5fgb9wf	279	5	liver	liver	NN
cord-007261-b5fgb9wf	279	6	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	279	7	apparatus	apparatus	NN
cord-007261-b5fgb9wf	279	8	:	:	:
cord-007261-b5fgb9wf	279	9	Separation	separation	NN
cord-007261-b5fgb9wf	279	10	of	of	IN
cord-007261-b5fgb9wf	279	11	enzyme	enzyme	NN
cord-007261-b5fgb9wf	279	12	activities	activity	NNS
cord-007261-b5fgb9wf	279	13	involved	involve	VBN
cord-007261-b5fgb9wf	279	14	in	in	IN
cord-007261-b5fgb9wf	279	15	the	the	DT
cord-007261-b5fgb9wf	279	16	biosynthesis	biosynthesis	NN
cord-007261-b5fgb9wf	279	17	of	of	IN
cord-007261-b5fgb9wf	279	18	the	the	DT
cord-007261-b5fgb9wf	279	19	phosphomannosyl	phosphomannosyl	JJ
cord-007261-b5fgb9wf	279	20	recognition	recognition	NN
cord-007261-b5fgb9wf	279	21	marker	marker	NN
cord-007261-b5fgb9wf	279	22	in	in	IN
cord-007261-b5fgb9wf	279	23	lysosomal	lysosomal	JJ
cord-007261-b5fgb9wf	279	24	enzymes	enzyme	NNS
cord-007261-b5fgb9wf	279	25	Phosphorylation	Phosphorylation	NNP
cord-007261-b5fgb9wf	279	26	of	of	IN
cord-007261-b5fgb9wf	279	27	hepatic	hepatic	JJ
cord-007261-b5fgb9wf	279	28	phenobarbital	phenobarbital	NN
cord-007261-b5fgb9wf	279	29	-	-	HYPH
cord-007261-b5fgb9wf	279	30	inducible	inducible	JJ
cord-007261-b5fgb9wf	279	31	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	279	32	P-450	p-450	XX
cord-007261-b5fgb9wf	280	1	Recycling	recycling	NN
cord-007261-b5fgb9wf	280	2	of	of	IN
cord-007261-b5fgb9wf	280	3	proteins	protein	NNS
cord-007261-b5fgb9wf	280	4	from	from	IN
cord-007261-b5fgb9wf	280	5	the	the	DT
cord-007261-b5fgb9wf	280	6	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	280	7	compartment	compartment	NN
cord-007261-b5fgb9wf	280	8	to	to	IN
cord-007261-b5fgb9wf	280	9	the	the	DT
cord-007261-b5fgb9wf	280	10	ER	ER	NNP
cord-007261-b5fgb9wf	280	11	in	in	IN
cord-007261-b5fgb9wf	280	12	yeast	yeast	NN
cord-007261-b5fgb9wf	280	13	Control	Control	NNP
cord-007261-b5fgb9wf	280	14	of	of	IN
cord-007261-b5fgb9wf	280	15	protein	protein	NN
cord-007261-b5fgb9wf	280	16	exit	exit	NN
cord-007261-b5fgb9wf	280	17	from	from	IN
cord-007261-b5fgb9wf	280	18	the	the	DT
cord-007261-b5fgb9wf	280	19	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	281	1	reticulum	reticulum	NN
cord-007261-b5fgb9wf	281	2	Iscytochrome	Iscytochrome	NNP
cord-007261-b5fgb9wf	281	3	P-450	P-450	VBD
cord-007261-b5fgb9wf	281	4	transported	transport	VBD
cord-007261-b5fgb9wf	281	5	from	from	IN
cord-007261-b5fgb9wf	281	6	the	the	DT
cord-007261-b5fgb9wf	281	7	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	281	8	reticulum	reticulum	NN
cord-007261-b5fgb9wf	281	9	to	to	IN
cord-007261-b5fgb9wf	281	10	the	the	DT
cord-007261-b5fgb9wf	281	11	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	281	12	apparatus	apparatus	NN
cord-007261-b5fgb9wf	281	13	in	in	IN
cord-007261-b5fgb9wf	281	14	rat	rat	NN
cord-007261-b5fgb9wf	281	15	hepatocytes	hepatocyte	NNS
cord-007261-b5fgb9wf	281	16	?	?	.
cord-007261-b5fgb9wf	282	1	An	an	DT
cord-007261-b5fgb9wf	282	2	N	n	CD
cord-007261-b5fgb9wf	282	3	-	-	HYPH
cord-007261-b5fgb9wf	282	4	tenninal	tenninal	JJ
cord-007261-b5fgb9wf	282	5	glycosylation	glycosylation	NN
cord-007261-b5fgb9wf	282	6	signal	signal	NN
cord-007261-b5fgb9wf	282	7	on	on	IN
cord-007261-b5fgb9wf	282	8	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	282	9	P450	p450	NN
cord-007261-b5fgb9wf	282	10	is	be	VBZ
cord-007261-b5fgb9wf	282	11	restricted	restrict	VBN
cord-007261-b5fgb9wf	282	12	to	to	IN
cord-007261-b5fgb9wf	282	13	the	the	DT
cord-007261-b5fgb9wf	282	14	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	282	15	reticulum	reticulum	NN
cord-007261-b5fgb9wf	282	16	in	in	IN
cord-007261-b5fgb9wf	282	17	a	a	DT
cord-007261-b5fgb9wf	282	18	luminal	luminal	JJ
cord-007261-b5fgb9wf	282	19	orientation	orientation	NN
cord-007261-b5fgb9wf	283	1	The	the	DT
cord-007261-b5fgb9wf	283	2	membrane	membrane	NN
cord-007261-b5fgb9wf	283	3	spanning	span	VBG
cord-007261-b5fgb9wf	283	4	domain	domain	NN
cord-007261-b5fgb9wf	283	5	of	of	IN
cord-007261-b5fgb9wf	283	6	y9-l,4-galflctosyltransferase	y9-l,4-galflctosyltransferase	NNP
cord-007261-b5fgb9wf	284	1	specifies	specify	VBZ
cord-007261-b5fgb9wf	284	2	trans	trans	NNP
cord-007261-b5fgb9wf	284	3	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	284	4	localization	localization	NN
cord-007261-b5fgb9wf	285	1	A	a	DT
cord-007261-b5fgb9wf	285	2	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	285	3	retention	retention	NN
cord-007261-b5fgb9wf	285	4	signal	signal	NN
cord-007261-b5fgb9wf	285	5	in	in	IN
cord-007261-b5fgb9wf	285	6	a	a	DT
cord-007261-b5fgb9wf	285	7	membrane	membrane	NN
cord-007261-b5fgb9wf	285	8	-	-	HYPH
cord-007261-b5fgb9wf	285	9	spanning	span	VBG
cord-007261-b5fgb9wf	285	10	domain	domain	NN
cord-007261-b5fgb9wf	285	11	of	of	IN
cord-007261-b5fgb9wf	285	12	coronavirus	coronavirus	NN
cord-007261-b5fgb9wf	285	13	El	El	NNP
cord-007261-b5fgb9wf	285	14	protein	protein	NN
cord-007261-b5fgb9wf	285	15	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	285	16	retention	retention	NN
cord-007261-b5fgb9wf	285	17	of	of	IN
cord-007261-b5fgb9wf	285	18	a	a	DT
cord-007261-b5fgb9wf	285	19	trans	trans	JJ
cord-007261-b5fgb9wf	285	20	-	-	JJ
cord-007261-b5fgb9wf	285	21	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	285	22	membrane	membrane	NN
cord-007261-b5fgb9wf	285	23	protein	protein	NN
cord-007261-b5fgb9wf	285	24	,	,	,
cord-007261-b5fgb9wf	285	25	galactosyl	galactosyl	NN
cord-007261-b5fgb9wf	285	26	-	-	HYPH
cord-007261-b5fgb9wf	285	27	transferase	transferase	NN
cord-007261-b5fgb9wf	285	28	,	,	,
cord-007261-b5fgb9wf	285	29	requires	require	VBZ
cord-007261-b5fgb9wf	285	30	cysteine	cysteine	NN
cord-007261-b5fgb9wf	285	31	and	and	CC
cord-007261-b5fgb9wf	285	32	histidine	histidine	NN
cord-007261-b5fgb9wf	285	33	residues	residue	NNS
cord-007261-b5fgb9wf	285	34	within	within	IN
cord-007261-b5fgb9wf	285	35	the	the	DT
cord-007261-b5fgb9wf	285	36	membrane	membrane	NN
cord-007261-b5fgb9wf	285	37	-	-	HYPH
cord-007261-b5fgb9wf	285	38	anchoring	anchor	VBG
cord-007261-b5fgb9wf	285	39	domain	domain	NN
cord-007261-b5fgb9wf	285	40	)	)	-RRB-
cord-007261-b5fgb9wf	286	1	ySl,4-Galactosyltransferase	ysl,4-galactosyltransferase	NN
cord-007261-b5fgb9wf	286	2	:	:	:
cord-007261-b5fgb9wf	287	1	A	a	DT
cord-007261-b5fgb9wf	287	2	short	short	JJ
cord-007261-b5fgb9wf	287	3	NH,-terminal	nh,-terminal	JJ
cord-007261-b5fgb9wf	287	4	fragment	fragment	NN
cord-007261-b5fgb9wf	287	5	that	that	WDT
cord-007261-b5fgb9wf	287	6	includes	include	VBZ
cord-007261-b5fgb9wf	287	7	the	the	DT
cord-007261-b5fgb9wf	287	8	cytoplasmic	cytoplasmic	JJ
cord-007261-b5fgb9wf	287	9	and	and	CC
cord-007261-b5fgb9wf	287	10	tranamembrane	tranamembrane	NNP
cord-007261-b5fgb9wf	287	11	domain	domain	NN
cord-007261-b5fgb9wf	287	12	is	be	VBZ
cord-007261-b5fgb9wf	287	13	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	287	14	for	for	IN
cord-007261-b5fgb9wf	287	15	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	287	16	retention	retention	NN
cord-007261-b5fgb9wf	288	1	The	the	DT
cord-007261-b5fgb9wf	288	2	signal	signal	NN
cord-007261-b5fgb9wf	288	3	for	for	IN
cord-007261-b5fgb9wf	288	4	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	288	5	retention	retention	NN
cord-007261-b5fgb9wf	288	6	of	of	IN
cord-007261-b5fgb9wf	288	7	bovine	bovine	NN
cord-007261-b5fgb9wf	288	8	/91,4-galactosyltransferase	/91,4-galactosyltransferase	,
cord-007261-b5fgb9wf	288	9	is	be	VBZ
cord-007261-b5fgb9wf	288	10	in	in	IN
cord-007261-b5fgb9wf	288	11	the	the	DT
cord-007261-b5fgb9wf	288	12	tranBmembrane	tranBmembrane	NNP
cord-007261-b5fgb9wf	288	13	domain	domain	NN
cord-007261-b5fgb9wf	288	14	Sequences	Sequences	NNPS
cord-007261-b5fgb9wf	288	15	within	within	IN
cord-007261-b5fgb9wf	288	16	and	and	CC
cord-007261-b5fgb9wf	288	17	adjacent	adjacent	JJ
cord-007261-b5fgb9wf	288	18	to	to	IN
cord-007261-b5fgb9wf	288	19	the	the	DT
cord-007261-b5fgb9wf	288	20	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	288	21	segment	segment	NN
cord-007261-b5fgb9wf	288	22	of	of	IN
cord-007261-b5fgb9wf	289	1	<	<	XX
cord-007261-b5fgb9wf	289	2	*	*	NFP
cord-007261-b5fgb9wf	289	3	-2,6-sialyltran8fera8e	-2,6-sialyltran8fera8e	NN
cord-007261-b5fgb9wf	289	4	specify	specify	VBP
cord-007261-b5fgb9wf	289	5	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	289	6	retention	retention	NN
cord-007261-b5fgb9wf	290	1	The	the	DT
cord-007261-b5fgb9wf	290	2	signal	signal	NN
cord-007261-b5fgb9wf	290	3	anchor	anchor	NN
cord-007261-b5fgb9wf	290	4	and	and	CC
cord-007261-b5fgb9wf	290	5	stem	stem	NN
cord-007261-b5fgb9wf	290	6	regions	region	NNS
cord-007261-b5fgb9wf	290	7	of	of	IN
cord-007261-b5fgb9wf	290	8	the	the	DT
cord-007261-b5fgb9wf	290	9	/J	/j	NN
cord-007261-b5fgb9wf	290	10	-	-	HYPH
cord-007261-b5fgb9wf	290	11	galactoaide	galactoaide	NNP
cord-007261-b5fgb9wf	291	1	<	<	XX
cord-007261-b5fgb9wf	292	1	*	*	NFP
cord-007261-b5fgb9wf	292	2	2,6-sialyltranBferase	2,6-sialyltranbferase	LS
cord-007261-b5fgb9wf	292	3	may	may	MD
cord-007261-b5fgb9wf	292	4	each	each	DT
cord-007261-b5fgb9wf	292	5	act	act	NN
cord-007261-b5fgb9wf	292	6	to	to	TO
cord-007261-b5fgb9wf	292	7	localize	localize	VB
cord-007261-b5fgb9wf	292	8	the	the	DT
cord-007261-b5fgb9wf	292	9	enzyme	enzyme	NN
cord-007261-b5fgb9wf	292	10	to	to	IN
cord-007261-b5fgb9wf	292	11	the	the	DT
cord-007261-b5fgb9wf	292	12	Golgi	Golgi	NNP
cord-007261-b5fgb9wf	292	13	apparatus	apparatus	NN
cord-007261-b5fgb9wf	292	14	The	the	DT
cord-007261-b5fgb9wf	292	15	transmembrane	transmembrane	NN
cord-007261-b5fgb9wf	292	16	and	and	CC
cord-007261-b5fgb9wf	292	17	flanUng	flanUng	NNP
cord-007261-b5fgb9wf	292	18	sequences	sequence	NNS
cord-007261-b5fgb9wf	292	19	of	of	IN
cord-007261-b5fgb9wf	292	20	/31,2-JV	/31,2-JV	NNP
cord-007261-b5fgb9wf	292	21	-	-	:
cord-007261-b5fgb9wf	292	22	acetylglucosaminyltransferase	acetylglucosaminyltransferase	NNP
cord-007261-b5fgb9wf	293	1	I	-PRON-	PRP
cord-007261-b5fgb9wf	293	2	specify	specify	VBP
cord-007261-b5fgb9wf	293	3	medial	medial	JJ
cord-007261-b5fgb9wf	293	4	-	-	HYPH
cord-007261-b5fgb9wf	293	5	Golgi	golgi	NN
cord-007261-b5fgb9wf	293	6	localization	localization	NN
cord-007261-b5fgb9wf	294	1	The	the	DT
cord-007261-b5fgb9wf	294	2	amino	amino	NN
cord-007261-b5fgb9wf	294	3	-	-	HYPH
cord-007261-b5fgb9wf	294	4	terminal	terminal	NN
cord-007261-b5fgb9wf	294	5	29	29	CD
cord-007261-b5fgb9wf	294	6	amino	amino	JJ
cord-007261-b5fgb9wf	294	7	acids	acid	NNS
cord-007261-b5fgb9wf	294	8	of	of	IN
cord-007261-b5fgb9wf	294	9	cytochrome	cytochrome	NN
cord-007261-b5fgb9wf	294	10	P450	p450	JJ
cord-007261-b5fgb9wf	294	11	2C1	2c1	CD
cord-007261-b5fgb9wf	294	12	are	be	VBP
cord-007261-b5fgb9wf	294	13	sufficient	sufficient	JJ
cord-007261-b5fgb9wf	294	14	for	for	IN
cord-007261-b5fgb9wf	294	15	retention	retention	NN
cord-007261-b5fgb9wf	294	16	in	in	IN
cord-007261-b5fgb9wf	294	17	the	the	DT
cord-007261-b5fgb9wf	294	18	endoplasmic	endoplasmic	NN
cord-007261-b5fgb9wf	294	19	reticulum	reticulum	NN