id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328082-7c7slfbp	nan	Effect of caffeine and reduced temperature (20 degrees C) on the organization of the pre-Golgi and the Golgi stack membranes	1993-03-02		.txt	text/plain	8889	403	56	This caffeine-induced effect on the Golgi complex was different from that induced by BFA, since mannosidase II, a Golgi stack marker, remained perinuclearly located and the Golgi stack coat protein, beta-COP, was not detached from Golgi membranes in the presence of 10 mM caffeine at 20 degrees C. Further, caffeine at reduced temperature did not inhibit the retrograde movement of Golgi stack membranes observed in BFA-treated cells. In control cells, the glycoproteins were transported to the trans-Golgi region already at 200C as described earlier (Marlin and Simons, 1983; Saraste and Kuismanen, 1984) , and at higher temperatures increased labeling of the plasma membrane was observed (data not shown). Because of the effective inhibition of membrane traffic out of the ER at 20~ and the translocation of p58 to the periphery of the cell in the presence of caffeine, it was of great interest to study whether the morphology of the Golgi stack is affected under the conditions used.	./cache/cord-328082-7c7slfbp.txt	./txt/cord-328082-7c7slfbp.txt