id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-264468-3oxzzxnd	Salcedo, Suzana P.	SseG, a virulence protein that targets Salmonella to the Golgi network	2003-10-01		.txt	text/plain	6930	371	51	Similar results were obtained using an antibody against TGN46, a glycoprotein the subcellular localization of GFP-expressing wild-type S.typhimurium (wt-GFP, green) in relation to the cis-Golgi protein giantin (red), and the host cell (DIC in merged image), 8 h after invasion. Since SseG is required for both Golgi localization of bacteria and intracellular replication, we hypothesized that the Golgi network might be exploited by Confocal immuno¯uorescence microscopy of HeLa cells infected for 10 h by GFP-expressing wild-type strain or the sseG mutant as a control. The protein was concentrated in a perinuclear region, where it co-localized extensively with Golgi markers including giantin (data not shown) and Cells expressing a myc-tagged version of SseG (green) were co-labelled with an antibody against TGN46 (red), and examined by confocal microscopy. HeLa cells expressing myc::SseG were incubated with BFA and ®xed for immuno¯uorescence The translocation of the SPI-2 TTSS effector SifA is required for recruitment of lgp-containing vesicles and the formation of Sifs, while SseG localizes SCVs to the Golgi network.	./cache/cord-264468-3oxzzxnd.txt	./txt/cord-264468-3oxzzxnd.txt