id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-291349-tq2n4mx3	Smith, Kevin R	Gene transfer in higher animals: theoretical considerations and key concepts	2002-10-09		.txt	text/plain	12232	661	45	The prospects for germline transgenesis via nuclear transfer (NT) are very significant: transgenes can be introduced to donor cells in vitro, permitting the production of genetically modified animals by NT . However, although retroviral-mediated gene therapy has been used successfully for (nonhuman) germline modifications, the most used */and to date the most useful */method for germline transgenesis is microinjection, reviewed in the following section. Because there are no special problems with microinjecting large transgene constructs, it is possible to incorporate structural gene-plus-promoter (plus other potentially useful sequences such as enhancers) combinations into the host genome. Therefore, the use of germline gene targeting as a means to avoiding transgene expression problems awaits progress in ES cell technology and NT technology. Following liposome-mediated gene transfer, amongst transgene molecules reaching the nucleus, only a minority integrate into the host cell chromosomes.	./cache/cord-291349-tq2n4mx3.txt	./txt/cord-291349-tq2n4mx3.txt