id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-336639-jaue41mv	Simons, Fermin A.	A mRNA PCR for the diagnosis of feline infectious peritonitis	2004-12-21		.txt	text/plain	3042	162	56	A reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of feline coronavirus (FCoV) messenger RNA in peripheral blood mononuclear cells (PBMCs) is described. The reason for this discrepancy became clear when the biological and genetic properties of FECV and FIPV isolates had been studied (Addie and Jarrett, 1992; Hohdatsu et al., 1992; Horzinek and Osterhaus, 1979) : the avirulent FCoV strains causing inconspicuous infections are responsible for the high seroprevalence; in cats experiencing some immunosuppressive event, expansion of the quasispecies cloud and mutations in the FECV genome lead to virulent variants that induce FIP (Vennema et al., 1998) . Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis	./cache/cord-336639-jaue41mv.txt	./txt/cord-336639-jaue41mv.txt