id author title date pages extension mime words sentences flesch summary cache txt cord-315094-pzixgqcy Benetka, Viviane Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis 2004-03-26 .txt text/plain 5537 282 60 Investigations showed that a high percentage of cats without FIP symptoms from exposed environments were positive for FCoV infection: 39-85% were seropositive, 37-95% viremic and 73-81% excreted virus in their faeces (Addie and Jarrett, 1992b; Sparkes et al., 1992; Herrewegh et al., 1995; Foley et al., 1997a,b; Gunn-Moore et al., 1998) . The recently developed reverse transcriptase polymerase chain reaction (RT-PCR) assays, using primers targeted to highly conserved regions of the viral genome (3 -UTR (untranslated region) (Herrewegh et al., 1995; Fehr et al., 1996) , or S-protein gene (Li and Scott, 1994; Gamble et al., 1997) ), which are common to all FCoV strains, became a valuable tool for the detection of FCoV nucleic acid in blood, body cavity effusions, faeces and tissue samples of infected cats. With the retrospective study presented here we investigated the prevalence of the two types of FCoVs in cats with histopathologically verified FIP using nested and seminested RT-PCR assays, with primers targeted as well to the S-protein gene. ./cache/cord-315094-pzixgqcy.txt ./txt/cord-315094-pzixgqcy.txt