id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353614-z4fpy607	Kusano, Nario	Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies	2007-02-16		.txt	text/plain	2976	147	54	title: Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Comparison of the sensitivities of ICA and DAS-ELISA Out of the three combinations of using three Mabs for ICA, clone 2G2 and clone 4E6, which had a different paratope group (PG), were more sensitive and suitable to the colloidal gold conjugate and coating antibody on the nitrocellulose strip for detection of SDV, respectively (data not shown). Suspensions of colloidal gold conjugate at various concentrations were applied to the reagent pad, and ICA The immunochromatographic devices were prepared under the following conditions: Case 3-1, 1 mg/ml of anti-SDV polyclonal antibody was deposited onto a nitrocellulose membrane to make a test line.	./cache/cord-353614-z4fpy607.txt	./txt/cord-353614-z4fpy607.txt