id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-334896-3g75spkc	MINAMI, Shohei	Establishment of serological test to detect antibody against ferret coronavirus	2016-03-03		.txt	text/plain	2493	150	59	Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. This different reactivity was also confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1–179 of the N protein was used as an ELISA antigen. In this study, an enzyme-linked immunosorbent assay (ELISA) using recombinant N proteins was established and applied to investigate the seroprevalence of FRcoV infection in Japan. The plasma of No.10 and serum of ferret No.22 showed differ-ent reactivities from the other samples in ELISA (Fig. 2) . In this study, we attempted to clarify the seroprevalence of FRcoV in Japan and developed an ELISA using two Yamaguchi-1 strain recombinant N proteins, GST-N (1-179) and GST-N (180-374).	./cache/cord-334896-3g75spkc.txt	./txt/cord-334896-3g75spkc.txt