id author title date pages extension mime words sentences flesch summary cache txt cord-305516-s1lvhknm Watanabe, Shumpei Epizootology and experimental infection of Yokose virus in bats 2008-09-11 .txt text/plain 4133 232 55 To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. To detect antibodies against YOKV, we developed an ELISA using biotinylated anti-bat IgG rabbit sera. Using the conventional ELISA, a serological survey was performed on bat serum samples collected from the Philippines and Malaysia. To obtain sera positive for anti-YOKV antibodies, two bats were immunized with inactivated and purified virus. Furthermore, the serum samples collected from bats which were obtained from Japan Zoos were also screened by ELISA using the JEV antigen. We developed an ELISA system using biotin-labeled anti-bat-IgG rabbit serum to detect antibodies against YOKV in bat sera and conducted serological surveys using this system. ./cache/cord-305516-s1lvhknm.txt ./txt/cord-305516-s1lvhknm.txt