id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-013348-lsksys56	Goto, Keiko	Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3	2020-09-19		.txt	text/plain	4742	247	50	title: Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3 From the resultant hybridoma clones, we selected nine clones producing mAbs reactive to the HPeV3-VP0 antigen, based on enzyme-linked immunosorbent assay (ELISA). Although RT-PCR-based diagnostic tests targeting 5 -UTR of the HPeV3 genome were developed for HPeV3 detection in clinical samples, there is currently no diagnostic method for detecting the viral antigens. To develop a rapid and effective diagnostic strategy, there is an urgent need to produce highly specific monoclonal antibodies (mAbs) toward HPeV3 antigens. As a result, we obtained nine mAb clones for characterization, and thereafter, generated an ELISA system that is specifically able to detect the HPeV3 VP0 antigens. We next performed antigen-capture ELISA with recombinant VP0 protein and virions released into the cell-culture supernatant of the HPeV3-infected cells. In this study, we sought to generate specific mAbs and develop an ELISA test for the detection of HPeV3 VP0 antigen.	./cache/cord-013348-lsksys56.txt	./txt/cord-013348-lsksys56.txt