id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003471-tr3ageky	Gaikwad, Satish S.	Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein	2019-01-31		.txt	text/plain	3398	199	51	PURPOSE: The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying the marker vaccine lacking an immunodominant epitope (IDE) of nucleoprotein of Newcastle disease virus (NDV). Epitope repeat protein (ERP) gene encoding eight repeats of an IDE sequence containing ETQFLDLMRAVANSMR (C-terminal IDE aa 444-459) on NDV NP separated by tetra-glycine linker was synthesized commercially using codon optimization for baculovirus expression. In addition to SPF chicken serum, different hyper-immune chicken sera to NDV, H9N2 avian influenza virus (AIV), infectious bronchitis virus (IBV), and infectious bursal disease virus (IBDV) were tested for specificity of rERP using an indirect ELISA assay. Second and third groups were sera taken 14 dpi from SPF chickens vaccinated with IDE deleted recombinant Newcastle disease virus (rNDV) [4] and NDV LaSota strain [19] , respectively, which kindly supplied by the OIE reference laboratory for ND, Korea.	./cache/cord-003471-tr3ageky.txt	./txt/cord-003471-tr3ageky.txt