id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005435-onghg1o8	Zhang, J	Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein–Barr virus-based episomal plasmid	2003-04-17		.txt	text/plain	2870	168	53	title: Prolonged gene expression in mouse lung endothelial cells following transfection with Epstein–Barr virus-based episomal plasmid In this study, we show that pulmonary gene transfer via cationic lipidic vectors can be significantly improved using an Epstein–Barr virus (EBV)-based expression plasmid. Systemic administration of cationic liposomes followed by the EBV-based plasmid led to gene expression in the lung that lasted for more than 3 weeks. Prolonged and high levels of gene expression can also be obtained in primary mouse lung endothelial cells (MLEC) following lipofection with an EBV-based plasmid. In the current study, we investigate whether in vitro and in vivo gene transfer to mouse lung endothelial cells (MLEC) can be significantly improved using an EBVbased expression plasmid. Furthermore, sequential injection of DOTAP:cholesterol liposomes and pGEG.GL3 led to a significantly higher level of gene expression in the lung than complex injection (Figure 2b ). Figure 5 Prolonged gene expression in primary lung endothelial cells following lipofection with an EBV-based plasmid.	./cache/cord-005435-onghg1o8.txt	./txt/cord-005435-onghg1o8.txt