id	sid	tid	token	lemma	pos
cord-347472-n6811ens	1	1	key	key	NN
cord-347472-n6811ens	1	2	:	:	:
cord-347472-n6811ens	2	1	cord-347472-n6811ens	cord-347472-n6811en	VBN
cord-347472-n6811ens	2	2	authors	author	NNS
cord-347472-n6811ens	2	3	:	:	:
cord-347472-n6811ens	2	4	Rosebrock	Rosebrock	NNP
cord-347472-n6811ens	2	5	,	,	,
cord-347472-n6811ens	2	6	Adam	Adam	NNP
cord-347472-n6811ens	2	7	P.	P.	NNP
cord-347472-n6811ens	2	8	title	title	NN
cord-347472-n6811ens	2	9	:	:	:
cord-347472-n6811ens	3	1	Patient	Patient	NNP
cord-347472-n6811ens	3	2	DNA	DNA	NNP
cord-347472-n6811ens	3	3	cross	cross	NN
cord-347472-n6811ens	3	4	-	-	NN
cord-347472-n6811ens	3	5	reactivity	reactivity	NN
cord-347472-n6811ens	3	6	of	of	IN
cord-347472-n6811ens	3	7	the	the	DT
cord-347472-n6811ens	3	8	CDC	CDC	NNP
cord-347472-n6811ens	3	9	SARS	SARS	NNP
cord-347472-n6811ens	3	10	-	-	HYPH
cord-347472-n6811ens	3	11	CoV-2	CoV-2	NNP
cord-347472-n6811ens	3	12	extraction	extraction	NN
cord-347472-n6811ens	3	13	control	control	NN
cord-347472-n6811ens	3	14	leads	lead	VBZ
cord-347472-n6811ens	3	15	to	to	IN
cord-347472-n6811ens	3	16	an	an	DT
cord-347472-n6811ens	3	17	inherent	inherent	JJ
cord-347472-n6811ens	3	18	potential	potential	NN
cord-347472-n6811ens	3	19	for	for	IN
cord-347472-n6811ens	3	20	false	false	JJ
cord-347472-n6811ens	3	21	negative	negative	JJ
cord-347472-n6811ens	3	22	results	result	NNS
cord-347472-n6811ens	3	23	date	date	NN
cord-347472-n6811ens	4	1	:	:	:
cord-347472-n6811ens	4	2	2020	2020	CD
cord-347472-n6811ens	4	3	-	-	HYPH
cord-347472-n6811ens	4	4	05	05	CD
cord-347472-n6811ens	4	5	-	-	SYM
cord-347472-n6811ens	4	6	15	15	CD
cord-347472-n6811ens	4	7	journal	journal	NN
cord-347472-n6811ens	4	8	:	:	:
cord-347472-n6811ens	5	1	bioRxiv	biorxiv	IN
cord-347472-n6811ens	5	2	DOI	DOI	NNP
cord-347472-n6811ens	5	3	:	:	:
cord-347472-n6811ens	6	1	10.1101/2020.05.13.094839	10.1101/2020.05.13.094839	CD
cord-347472-n6811ens	7	1	sha	sha	NNP
cord-347472-n6811ens	7	2	:	:	:
cord-347472-n6811ens	8	1	cbd17d4433d547a8641842b57888e4a19f45cc20	cbd17d4433d547a8641842b57888e4a19f45cc20	NNP
cord-347472-n6811ens	8	2	doc_id	doc_id	CD
cord-347472-n6811ens	8	3	:	:	:
cord-347472-n6811ens	9	1	347472	347472	CD
cord-347472-n6811ens	9	2	cord_uid	cord_uid	NN
cord-347472-n6811ens	9	3	:	:	:
cord-347472-n6811ens	10	1	n6811ens	n6811ens	NNP
cord-347472-n6811ens	10	2	Testing	Testing	NNP
cord-347472-n6811ens	10	3	for	for	IN
cord-347472-n6811ens	10	4	RNA	RNA	NNP
cord-347472-n6811ens	10	5	viruses	virus	NNS
cord-347472-n6811ens	10	6	such	such	JJ
cord-347472-n6811ens	10	7	as	as	IN
cord-347472-n6811ens	10	8	SARS	SARS	NNP
cord-347472-n6811ens	10	9	-	-	HYPH
cord-347472-n6811ens	10	10	CoV-2	CoV-2	NNP
cord-347472-n6811ens	10	11	requires	require	VBZ
cord-347472-n6811ens	10	12	careful	careful	JJ
cord-347472-n6811ens	10	13	handling	handling	NN
cord-347472-n6811ens	10	14	of	of	IN
cord-347472-n6811ens	10	15	inherently	inherently	RB
cord-347472-n6811ens	10	16	labile	labile	JJ
cord-347472-n6811ens	10	17	RNA	RNA	NNP
cord-347472-n6811ens	10	18	during	during	IN
cord-347472-n6811ens	10	19	sample	sample	NN
cord-347472-n6811ens	10	20	collection	collection	NN
cord-347472-n6811ens	10	21	,	,	,
cord-347472-n6811ens	10	22	clinical	clinical	JJ
cord-347472-n6811ens	10	23	processing	processing	NN
cord-347472-n6811ens	10	24	,	,	,
cord-347472-n6811ens	10	25	and	and	CC
cord-347472-n6811ens	10	26	molecular	molecular	JJ
cord-347472-n6811ens	10	27	analysis	analysis	NN
cord-347472-n6811ens	10	28	.	.	.
cord-347472-n6811ens	11	1	Tests	test	NNS
cord-347472-n6811ens	11	2	must	must	MD
cord-347472-n6811ens	11	3	include	include	VB
cord-347472-n6811ens	11	4	fail	fail	VB
cord-347472-n6811ens	11	5	-	-	HYPH
cord-347472-n6811ens	11	6	safe	safe	JJ
cord-347472-n6811ens	11	7	controls	control	NNS
cord-347472-n6811ens	11	8	that	that	WDT
cord-347472-n6811ens	11	9	affirmatively	affirmatively	RB
cord-347472-n6811ens	11	10	report	report	VBP
cord-347472-n6811ens	11	11	the	the	DT
cord-347472-n6811ens	11	12	presence	presence	NN
cord-347472-n6811ens	11	13	of	of	IN
cord-347472-n6811ens	11	14	intact	intact	JJ
cord-347472-n6811ens	11	15	RNA	RNA	NNP
cord-347472-n6811ens	11	16	and	and	CC
cord-347472-n6811ens	11	17	demonstrate	demonstrate	VB
cord-347472-n6811ens	11	18	success	success	NN
cord-347472-n6811ens	11	19	of	of	IN
cord-347472-n6811ens	11	20	all	all	DT
cord-347472-n6811ens	11	21	steps	step	NNS
cord-347472-n6811ens	11	22	of	of	IN
cord-347472-n6811ens	11	23	the	the	DT
cord-347472-n6811ens	11	24	assay	assay	NN
cord-347472-n6811ens	11	25	.	.	.
cord-347472-n6811ens	12	1	A	a	DT
cord-347472-n6811ens	12	2	result	result	NN
cord-347472-n6811ens	12	3	of	of	IN
cord-347472-n6811ens	12	4	“	"	``
cord-347472-n6811ens	12	5	no	no	DT
cord-347472-n6811ens	12	6	virus	virus	NN
cord-347472-n6811ens	12	7	signal	signal	NN
cord-347472-n6811ens	12	8	”	"	''
cord-347472-n6811ens	12	9	is	be	VBZ
cord-347472-n6811ens	12	10	insufficient	insufficient	JJ
cord-347472-n6811ens	12	11	for	for	IN
cord-347472-n6811ens	12	12	clinical	clinical	JJ
cord-347472-n6811ens	12	13	interpretation	interpretation	NN
cord-347472-n6811ens	12	14	:	:	:
cord-347472-n6811ens	12	15	controls	control	NNS
cord-347472-n6811ens	12	16	must	must	MD
cord-347472-n6811ens	12	17	also	also	RB
cord-347472-n6811ens	12	18	say	say	VB
cord-347472-n6811ens	12	19	“	"	``
cord-347472-n6811ens	12	20	The	the	DT
cord-347472-n6811ens	12	21	reaction	reaction	NN
cord-347472-n6811ens	12	22	worked	work	VBD
cord-347472-n6811ens	12	23	as	as	IN
cord-347472-n6811ens	12	24	intended	intend	VBN
cord-347472-n6811ens	12	25	and	and	CC
cord-347472-n6811ens	12	26	would	would	MD
cord-347472-n6811ens	12	27	have	have	VB
cord-347472-n6811ens	12	28	found	find	VBN
cord-347472-n6811ens	12	29	virus	virus	NN
cord-347472-n6811ens	12	30	if	if	IN
cord-347472-n6811ens	12	31	present	present	JJ
cord-347472-n6811ens	12	32	.	.	.
cord-347472-n6811ens	12	33	”	"	''
cord-347472-n6811ens	13	1	Unfortunately	unfortunately	RB
cord-347472-n6811ens	13	2	,	,	,
cord-347472-n6811ens	13	3	a	a	DT
cord-347472-n6811ens	13	4	widely	widely	RB
cord-347472-n6811ens	13	5	used	use	VBN
cord-347472-n6811ens	13	6	test	test	NN
cord-347472-n6811ens	13	7	specified	specify	VBN
cord-347472-n6811ens	13	8	by	by	IN
cord-347472-n6811ens	13	9	the	the	DT
cord-347472-n6811ens	13	10	US	US	NNP
cord-347472-n6811ens	13	11	Centers	Centers	NNPS
cord-347472-n6811ens	13	12	for	for	IN
cord-347472-n6811ens	13	13	Disease	Disease	NNP
cord-347472-n6811ens	13	14	Control	Control	NNP
cord-347472-n6811ens	13	15	and	and	CC
cord-347472-n6811ens	13	16	Prevention	Prevention	NNP
cord-347472-n6811ens	13	17	(	(	-LRB-
cord-347472-n6811ens	13	18	CDC	CDC	NNP
cord-347472-n6811ens	13	19	)	)	-RRB-
cord-347472-n6811ens	13	20	incorporates	incorporate	VBZ
cord-347472-n6811ens	13	21	a	a	DT
cord-347472-n6811ens	13	22	control	control	NN
cord-347472-n6811ens	13	23	that	that	WDT
cord-347472-n6811ens	13	24	does	do	VBZ
cord-347472-n6811ens	13	25	not	not	RB
cord-347472-n6811ens	13	26	perform	perform	VB
cord-347472-n6811ens	13	27	as	as	IN
cord-347472-n6811ens	13	28	intended	intend	VBN
cord-347472-n6811ens	13	29	and	and	CC
cord-347472-n6811ens	13	30	claimed	claim	VBN
cord-347472-n6811ens	13	31	.	.	.
cord-347472-n6811ens	14	1	Detecting	detect	VBG
cord-347472-n6811ens	14	2	SARS	SARS	NNP
cord-347472-n6811ens	14	3	-	-	HYPH
cord-347472-n6811ens	14	4	CoV-2	CoV-2	NNP
cord-347472-n6811ens	14	5	with	with	IN
cord-347472-n6811ens	14	6	this	this	DT
cord-347472-n6811ens	14	7	assay	assay	NN
cord-347472-n6811ens	14	8	requires	require	VBZ
cord-347472-n6811ens	14	9	both	both	DT
cord-347472-n6811ens	14	10	intact	intact	JJ
cord-347472-n6811ens	14	11	RNA	RNA	NNP
cord-347472-n6811ens	14	12	and	and	CC
cord-347472-n6811ens	14	13	successful	successful	JJ
cord-347472-n6811ens	14	14	reverse	reverse	NN
cord-347472-n6811ens	14	15	transcription	transcription	NN
cord-347472-n6811ens	14	16	.	.	.
cord-347472-n6811ens	15	1	The	the	DT
cord-347472-n6811ens	15	2	CDC	CDC	NNP
cord-347472-n6811ens	15	3	-	-	HYPH
cord-347472-n6811ens	15	4	specified	specify	VBN
cord-347472-n6811ens	15	5	control	control	NN
cord-347472-n6811ens	15	6	does	do	VBZ
cord-347472-n6811ens	15	7	not	not	RB
cord-347472-n6811ens	15	8	require	require	VB
cord-347472-n6811ens	15	9	either	either	DT
cord-347472-n6811ens	15	10	of	of	IN
cord-347472-n6811ens	15	11	these	these	DT
cord-347472-n6811ens	15	12	,	,	,
cord-347472-n6811ens	15	13	due	due	IN
cord-347472-n6811ens	15	14	to	to	IN
cord-347472-n6811ens	15	15	its	-PRON-	PRP$
cord-347472-n6811ens	15	16	inability	inability	NN
cord-347472-n6811ens	15	17	to	to	TO
cord-347472-n6811ens	15	18	differentiate	differentiate	VB
cord-347472-n6811ens	15	19	human	human	JJ
cord-347472-n6811ens	15	20	genomic	genomic	JJ
cord-347472-n6811ens	15	21	DNA	dna	NN
cord-347472-n6811ens	15	22	from	from	IN
cord-347472-n6811ens	15	23	reverse	reverse	RB
cord-347472-n6811ens	15	24	-	-	HYPH
cord-347472-n6811ens	15	25	transcribed	transcribe	VBN
cord-347472-n6811ens	15	26	RNA	RNA	NNP
cord-347472-n6811ens	15	27	.	.	.
cord-347472-n6811ens	16	1	Patient	Patient	NNP
cord-347472-n6811ens	16	2	DNA	DNA	NNP
cord-347472-n6811ens	16	3	is	be	VBZ
cord-347472-n6811ens	16	4	copurified	copurifie	VBN
cord-347472-n6811ens	16	5	from	from	IN
cord-347472-n6811ens	16	6	nasopharyngeal	nasopharyngeal	JJ
cord-347472-n6811ens	16	7	swabs	swab	NNS
cord-347472-n6811ens	16	8	during	during	IN
cord-347472-n6811ens	16	9	clinically	clinically	RB
cord-347472-n6811ens	16	10	-	-	HYPH
cord-347472-n6811ens	16	11	approved	approve	VBN
cord-347472-n6811ens	16	12	RNA	RNA	NNP
cord-347472-n6811ens	16	13	extraction	extraction	NN
cord-347472-n6811ens	16	14	and	and	CC
cord-347472-n6811ens	16	15	is	be	VBZ
cord-347472-n6811ens	16	16	sufficient	sufficient	JJ
cord-347472-n6811ens	16	17	to	to	TO
cord-347472-n6811ens	16	18	return	return	VB
cord-347472-n6811ens	16	19	an	an	DT
cord-347472-n6811ens	16	20	“	"	``
cord-347472-n6811ens	16	21	extraction	extraction	NN
cord-347472-n6811ens	16	22	control	control	NN
cord-347472-n6811ens	16	23	success	success	NN
cord-347472-n6811ens	16	24	”	"	''
cord-347472-n6811ens	16	25	signal	signal	NN
cord-347472-n6811ens	16	26	using	use	VBG
cord-347472-n6811ens	16	27	the	the	DT
cord-347472-n6811ens	16	28	CDC	CDC	NNP
cord-347472-n6811ens	16	29	design	design	NN
cord-347472-n6811ens	16	30	.	.	.
cord-347472-n6811ens	17	1	As	as	IN
cord-347472-n6811ens	17	2	such	such	JJ
cord-347472-n6811ens	17	3	,	,	,
cord-347472-n6811ens	17	4	this	this	DT
cord-347472-n6811ens	17	5	assay	assay	NN
cord-347472-n6811ens	17	6	fails	fail	VBZ
cord-347472-n6811ens	17	7	-	-	HYPH
cord-347472-n6811ens	17	8	unsafe	unsafe	JJ
cord-347472-n6811ens	17	9	:	:	:
cord-347472-n6811ens	17	10	truly	truly	RB
cord-347472-n6811ens	17	11	positive	positive	JJ
cord-347472-n6811ens	17	12	patient	patient	NN
cord-347472-n6811ens	17	13	samples	sample	NNS
cord-347472-n6811ens	17	14	return	return	VBP
cord-347472-n6811ens	17	15	a	a	DT
cord-347472-n6811ens	17	16	false	false	JJ
cord-347472-n6811ens	17	17	-	-	HYPH
cord-347472-n6811ens	17	18	negative	negative	JJ
cord-347472-n6811ens	17	19	result	result	NN
cord-347472-n6811ens	17	20	of	of	IN
cord-347472-n6811ens	17	21	“	"	``
cord-347472-n6811ens	17	22	no	no	DT
cord-347472-n6811ens	17	23	virus	virus	NN
cord-347472-n6811ens	17	24	detected	detect	VBN
cord-347472-n6811ens	17	25	,	,	,
cord-347472-n6811ens	17	26	control	control	NN
cord-347472-n6811ens	17	27	succeeded	succeed	VBD
cord-347472-n6811ens	17	28	”	"	''
cord-347472-n6811ens	17	29	following	follow	VBG
cord-347472-n6811ens	17	30	any	any	DT
cord-347472-n6811ens	17	31	of	of	IN
cord-347472-n6811ens	17	32	several	several	JJ
cord-347472-n6811ens	17	33	readily	readily	RB
cord-347472-n6811ens	17	34	-	-	HYPH
cord-347472-n6811ens	17	35	encountered	encounter	VBN
cord-347472-n6811ens	17	36	mishaps	mishap	NNS
cord-347472-n6811ens	17	37	.	.	.
cord-347472-n6811ens	18	1	This	this	DT
cord-347472-n6811ens	18	2	problem	problem	NN
cord-347472-n6811ens	18	3	affects	affect	VBZ
cord-347472-n6811ens	18	4	tens	ten	NNS
cord-347472-n6811ens	18	5	-	-	HYPH
cord-347472-n6811ens	18	6	of	of	IN
cord-347472-n6811ens	18	7	-	-	HYPH
cord-347472-n6811ens	18	8	millions	million	NNS
cord-347472-n6811ens	18	9	of	of	IN
cord-347472-n6811ens	18	10	patients	patient	NNS
cord-347472-n6811ens	18	11	worth	worth	JJ
cord-347472-n6811ens	18	12	of	of	IN
cord-347472-n6811ens	18	13	shipped	ship	VBN
cord-347472-n6811ens	18	14	assays	assay	NNS
cord-347472-n6811ens	18	15	,	,	,
cord-347472-n6811ens	18	16	but	but	CC
cord-347472-n6811ens	18	17	many	many	JJ
cord-347472-n6811ens	18	18	of	of	IN
cord-347472-n6811ens	18	19	these	these	DT
cord-347472-n6811ens	18	20	flawed	flawed	JJ
cord-347472-n6811ens	18	21	reagents	reagent	NNS
cord-347472-n6811ens	18	22	have	have	VBP
cord-347472-n6811ens	18	23	not	not	RB
cord-347472-n6811ens	18	24	yet	yet	RB
cord-347472-n6811ens	18	25	been	be	VBN
cord-347472-n6811ens	18	26	used	use	VBN
cord-347472-n6811ens	18	27	.	.	.
cord-347472-n6811ens	19	1	There	there	EX
cord-347472-n6811ens	19	2	is	be	VBZ
cord-347472-n6811ens	19	3	an	an	DT
cord-347472-n6811ens	19	4	opportunity	opportunity	NN
cord-347472-n6811ens	19	5	to	to	TO
cord-347472-n6811ens	19	6	improve	improve	VB
cord-347472-n6811ens	19	7	this	this	DT
cord-347472-n6811ens	19	8	important	important	JJ
cord-347472-n6811ens	19	9	diagnostic	diagnostic	JJ
cord-347472-n6811ens	19	10	tool	tool	NN
cord-347472-n6811ens	19	11	.	.	.
cord-347472-n6811ens	20	1	As	as	IN
cord-347472-n6811ens	20	2	demonstrated	demonstrate	VBN
cord-347472-n6811ens	20	3	here	here	RB
cord-347472-n6811ens	20	4	,	,	,
cord-347472-n6811ens	20	5	a	a	DT
cord-347472-n6811ens	20	6	re	re	NN
cord-347472-n6811ens	20	7	-	-	JJ
cord-347472-n6811ens	20	8	designed	design	VBN
cord-347472-n6811ens	20	9	transcript	transcript	NN
cord-347472-n6811ens	20	10	-	-	HYPH
cord-347472-n6811ens	20	11	specific	specific	JJ
cord-347472-n6811ens	20	12	control	control	NN
cord-347472-n6811ens	20	13	correctly	correctly	RB
cord-347472-n6811ens	20	14	monitors	monitor	VBZ
cord-347472-n6811ens	20	15	sample	sample	NN
cord-347472-n6811ens	20	16	collection	collection	NN
cord-347472-n6811ens	20	17	,	,	,
cord-347472-n6811ens	20	18	extraction	extraction	NN
cord-347472-n6811ens	20	19	,	,	,
cord-347472-n6811ens	20	20	reverse	reverse	JJ
cord-347472-n6811ens	20	21	transcription	transcription	NN
cord-347472-n6811ens	20	22	,	,	,
cord-347472-n6811ens	20	23	and	and	CC
cord-347472-n6811ens	20	24	qPCR	qPCR	NNP
cord-347472-n6811ens	20	25	detection	detection	NN
cord-347472-n6811ens	20	26	.	.	.
cord-347472-n6811ens	21	1	This	this	DT
cord-347472-n6811ens	21	2	approach	approach	NN
cord-347472-n6811ens	21	3	can	can	MD
cord-347472-n6811ens	21	4	be	be	VB
cord-347472-n6811ens	21	5	rapidly	rapidly	RB
cord-347472-n6811ens	21	6	implemented	implement	VBN
cord-347472-n6811ens	21	7	and	and	CC
cord-347472-n6811ens	21	8	will	will	MD
cord-347472-n6811ens	21	9	help	help	VB
cord-347472-n6811ens	21	10	reduce	reduce	VB
cord-347472-n6811ens	21	11	truly	truly	RB
cord-347472-n6811ens	21	12	positive	positive	JJ
cord-347472-n6811ens	21	13	patients	patient	NNS
cord-347472-n6811ens	21	14	from	from	IN
cord-347472-n6811ens	21	15	being	be	VBG
cord-347472-n6811ens	21	16	incorrectly	incorrectly	RB
cord-347472-n6811ens	21	17	given	give	VBN
cord-347472-n6811ens	21	18	the	the	DT
cord-347472-n6811ens	21	19	all	all	RB
cord-347472-n6811ens	21	20	-	-	HYPH
cord-347472-n6811ens	21	21	clear	clear	JJ
cord-347472-n6811ens	21	22	.	.	.
cord-347472-n6811ens	22	1	One	one	CD
cord-347472-n6811ens	22	2	Sentence	sentence	NN
cord-347472-n6811ens	22	3	Summary	Summary	NNP
cord-347472-n6811ens	22	4	A	a	DT
cord-347472-n6811ens	22	5	widely	widely	RB
cord-347472-n6811ens	22	6	-	-	HYPH
cord-347472-n6811ens	22	7	used	use	VBN
cord-347472-n6811ens	22	8	COVID-19	COVID-19	NNP
cord-347472-n6811ens	22	9	diagnostic	diagnostic	NNP
cord-347472-n6811ens	22	10	is	be	VBZ
cord-347472-n6811ens	22	11	mis	mi	VBN
cord-347472-n6811ens	22	12	-	-	HYPH
cord-347472-n6811ens	22	13	designed	design	VBN
cord-347472-n6811ens	22	14	and	and	CC
cord-347472-n6811ens	22	15	generates	generate	VBZ
cord-347472-n6811ens	22	16	false	false	JJ
cord-347472-n6811ens	22	17	-	-	HYPH
cord-347472-n6811ens	22	18	negative	negative	JJ
cord-347472-n6811ens	22	19	results	result	NNS
cord-347472-n6811ens	22	20	,	,	,
cord-347472-n6811ens	22	21	dangerously	dangerously	RB
cord-347472-n6811ens	22	22	confusing	confusing	JJ
cord-347472-n6811ens	22	23	“	"	``
cord-347472-n6811ens	22	24	No	no	UH
cord-347472-n6811ens	22	25	”	"	''
cord-347472-n6811ens	22	26	with	with	IN
cord-347472-n6811ens	22	27	“	"	``
cord-347472-n6811ens	22	28	Do	do	VBP
cord-347472-n6811ens	22	29	n’t	not	RB
cord-347472-n6811ens	22	30	know	know	VB
cord-347472-n6811ens	22	31	”	"	''
cord-347472-n6811ens	22	32	–	–	:
cord-347472-n6811ens	22	33	but	but	CC
cord-347472-n6811ens	22	34	it	-PRON-	PRP
cord-347472-n6811ens	22	35	’s	’	VBZ
cord-347472-n6811ens	22	36	fixable	fixable	JJ
cord-347472-n6811ens	23	1	the	the	DT
cord-347472-n6811ens	23	2	focus	focus	NN
cord-347472-n6811ens	23	3	of	of	IN
cord-347472-n6811ens	23	4	molecular	molecular	JJ
cord-347472-n6811ens	23	5	testing	testing	NN
cord-347472-n6811ens	23	6	will	will	MD
cord-347472-n6811ens	23	7	shift	shift	VB
cord-347472-n6811ens	23	8	to	to	IN
cord-347472-n6811ens	23	9	clearing	clear	VBG
cord-347472-n6811ens	23	10	asymptomatic	asymptomatic	JJ
cord-347472-n6811ens	23	11	individuals	individual	NNS
cord-347472-n6811ens	23	12	based	base	VBN
cord-347472-n6811ens	23	13	on	on	IN
cord-347472-n6811ens	23	14	absence	absence	NN
cord-347472-n6811ens	23	15	of	of	IN
cord-347472-n6811ens	23	16	detectable	detectable	JJ
cord-347472-n6811ens	23	17	virus	virus	NN
cord-347472-n6811ens	23	18	.	.	.
cord-347472-n6811ens	24	1	The	the	DT
cord-347472-n6811ens	24	2	US	US	NNP
cord-347472-n6811ens	24	3	Centers	Centers	NNPS
cord-347472-n6811ens	24	4	for	for	IN
cord-347472-n6811ens	24	5	Disease	Disease	NNP
cord-347472-n6811ens	24	6	Control	Control	NNP
cord-347472-n6811ens	24	7	and	and	CC
cord-347472-n6811ens	24	8	Prevention	Prevention	NNP
cord-347472-n6811ens	24	9	(	(	-LRB-
cord-347472-n6811ens	24	10	CDC	CDC	NNP
cord-347472-n6811ens	24	11	)	)	-RRB-
cord-347472-n6811ens	24	12	have	have	VBP
cord-347472-n6811ens	24	13	specified	specify	VBN
cord-347472-n6811ens	24	14	and	and	CC
cord-347472-n6811ens	24	15	given	give	VBN
cord-347472-n6811ens	24	16	emergency	emergency	NN
cord-347472-n6811ens	24	17	use	use	NN
cord-347472-n6811ens	24	18	authorization	authorization	NN
cord-347472-n6811ens	24	19	(	(	-LRB-
cord-347472-n6811ens	24	20	EUA	EUA	NNP
cord-347472-n6811ens	24	21	)	)	-RRB-
cord-347472-n6811ens	24	22	for	for	IN
cord-347472-n6811ens	24	23	a	a	DT
cord-347472-n6811ens	24	24	SARS	SARS	NNP
cord-347472-n6811ens	24	25	-	-	HYPH
cord-347472-n6811ens	24	26	CoV-2	CoV-2	NNP
cord-347472-n6811ens	24	27	molecular	molecular	JJ
cord-347472-n6811ens	24	28	diagnostic	diagnostic	NN
cord-347472-n6811ens	24	29	used	use	VBN
cord-347472-n6811ens	24	30	to	to	TO
cord-347472-n6811ens	24	31	detect	detect	VB
cord-347472-n6811ens	24	32	viral	viral	JJ
cord-347472-n6811ens	24	33	RNA	rna	NN
cord-347472-n6811ens	24	34	in	in	IN
cord-347472-n6811ens	24	35	clinical	clinical	JJ
cord-347472-n6811ens	24	36	samples	sample	NNS
cord-347472-n6811ens	24	37	(	(	-LRB-
cord-347472-n6811ens	24	38	2	2	CD
cord-347472-n6811ens	24	39	)	)	-RRB-
cord-347472-n6811ens	24	40	.	.	.
cord-347472-n6811ens	25	1	This	this	DT
cord-347472-n6811ens	25	2	assay	assay	NN
cord-347472-n6811ens	25	3	is	be	VBZ
cord-347472-n6811ens	25	4	widely	widely	RB
cord-347472-n6811ens	25	5	used	use	VBN
cord-347472-n6811ens	25	6	and	and	CC
cord-347472-n6811ens	25	7	also	also	RB
cord-347472-n6811ens	25	8	serves	serve	VBZ
cord-347472-n6811ens	25	9	as	as	IN
cord-347472-n6811ens	25	10	a	a	DT
cord-347472-n6811ens	25	11	prototype	prototype	NN
cord-347472-n6811ens	25	12	for	for	IN
cord-347472-n6811ens	25	13	many	many	JJ
cord-347472-n6811ens	25	14	FDAauthorized	fdaauthorize	VBN
cord-347472-n6811ens	25	15	tests	test	NNS
cord-347472-n6811ens	25	16	(	(	-LRB-
cord-347472-n6811ens	25	17	discussed	discuss	VBN
cord-347472-n6811ens	25	18	below	below	RB
cord-347472-n6811ens	25	19	)	)	-RRB-
cord-347472-n6811ens	25	20	.	.	.
cord-347472-n6811ens	26	1	As	as	IN
cord-347472-n6811ens	26	2	I	-PRON-	PRP
cord-347472-n6811ens	26	3	show	show	VBP
cord-347472-n6811ens	26	4	here	here	RB
cord-347472-n6811ens	26	5	,	,	,
cord-347472-n6811ens	26	6	this	this	DT
cord-347472-n6811ens	26	7	assay	assay	NN
cord-347472-n6811ens	26	8	uses	use	VBZ
cord-347472-n6811ens	26	9	a	a	DT
cord-347472-n6811ens	26	10	poorly	poorly	RB
cord-347472-n6811ens	26	11	-	-	HYPH
cord-347472-n6811ens	26	12	designed	design	VBN
cord-347472-n6811ens	26	13	specimen	specimen	JJ
cord-347472-n6811ens	26	14	extraction	extraction	NN
cord-347472-n6811ens	26	15	control	control	NN
cord-347472-n6811ens	26	16	that	that	WDT
cord-347472-n6811ens	26	17	does	do	VBZ
cord-347472-n6811ens	26	18	not	not	RB
cord-347472-n6811ens	26	19	perform	perform	VB
cord-347472-n6811ens	26	20	as	as	IN
cord-347472-n6811ens	26	21	intended	intend	VBN
cord-347472-n6811ens	26	22	on	on	IN
cord-347472-n6811ens	26	23	real	real	JJ
cord-347472-n6811ens	26	24	-	-	HYPH
cord-347472-n6811ens	26	25	world	world	NN
cord-347472-n6811ens	26	26	samples	sample	NNS
cord-347472-n6811ens	26	27	.	.	.
cord-347472-n6811ens	27	1	The	the	DT
cord-347472-n6811ens	27	2	CDC	CDC	NNP
cord-347472-n6811ens	27	3	control	control	NN
cord-347472-n6811ens	27	4	fails	fail	VBZ
cord-347472-n6811ens	27	5	to	to	IN
cord-347472-n6811ens	27	6	reports	report	NNS
cord-347472-n6811ens	27	7	on	on	IN
cord-347472-n6811ens	27	8	specimen	specimen	NN
cord-347472-n6811ens	27	9	and	and	CC
cord-347472-n6811ens	27	10	process	process	NN
cord-347472-n6811ens	27	11	integrity	integrity	NN
cord-347472-n6811ens	27	12	.	.	.
cord-347472-n6811ens	28	1	I	-PRON-	PRP
cord-347472-n6811ens	28	2	alerted	alert	VBD
cord-347472-n6811ens	28	3	the	the	DT
cord-347472-n6811ens	28	4	CDC	CDC	NNP
cord-347472-n6811ens	28	5	to	to	IN
cord-347472-n6811ens	28	6	this	this	DT
cord-347472-n6811ens	28	7	potential	potential	JJ
cord-347472-n6811ens	28	8	problem	problem	NN
cord-347472-n6811ens	28	9	in	in	IN
cord-347472-n6811ens	28	10	March	March	NNP
cord-347472-n6811ens	28	11	of	of	IN
cord-347472-n6811ens	28	12	2020	2020	CD
cord-347472-n6811ens	28	13	,	,	,
cord-347472-n6811ens	28	14	supported	support	VBN
cord-347472-n6811ens	28	15	by	by	IN
cord-347472-n6811ens	28	16	bioinformatic	bioinformatic	JJ
cord-347472-n6811ens	28	17	prediction	prediction	NN
cord-347472-n6811ens	28	18	.	.	.
cord-347472-n6811ens	29	1	Their	-PRON-	PRP$
cord-347472-n6811ens	29	2	response	response	NN
cord-347472-n6811ens	29	3	,	,	,
cord-347472-n6811ens	29	4	sent	send	VBD
cord-347472-n6811ens	29	5	more	more	JJR
cord-347472-n6811ens	29	6	than	than	IN
cord-347472-n6811ens	29	7	a	a	DT
cord-347472-n6811ens	29	8	month	month	NN
cord-347472-n6811ens	29	9	later	later	RB
cord-347472-n6811ens	29	10	,	,	,
cord-347472-n6811ens	29	11	did	do	VBD
cord-347472-n6811ens	29	12	not	not	RB
cord-347472-n6811ens	29	13	share	share	VB
cord-347472-n6811ens	29	14	my	-PRON-	PRP$
cord-347472-n6811ens	29	15	concerns	concern	NNS
cord-347472-n6811ens	29	16	.	.	.
cord-347472-n6811ens	30	1	One	one	CD
cord-347472-n6811ens	30	2	,	,	,
cord-347472-n6811ens	30	3	the	the	DT
cord-347472-n6811ens	30	4	CDC	CDC	NNP
cord-347472-n6811ens	30	5	had	have	VBD
cord-347472-n6811ens	30	6	not	not	RB
cord-347472-n6811ens	30	7	received	receive	VBN
cord-347472-n6811ens	30	8	widespread	widespread	JJ
cord-347472-n6811ens	30	9	reports	report	NNS
cord-347472-n6811ens	30	10	of	of	IN
cord-347472-n6811ens	30	11	false	false	JJ
cord-347472-n6811ens	30	12	-	-	HYPH
cord-347472-n6811ens	30	13	negative	negative	JJ
cord-347472-n6811ens	30	14	tests	test	NNS
cord-347472-n6811ens	30	15	.	.	.
cord-347472-n6811ens	31	1	Two	two	CD
cord-347472-n6811ens	31	2	,	,	,
cord-347472-n6811ens	31	3	negative	negative	JJ
cord-347472-n6811ens	31	4	patients	patient	NNS
cord-347472-n6811ens	31	5	should	should	MD
cord-347472-n6811ens	31	6	err	err	VB
cord-347472-n6811ens	31	7	on	on	IN
cord-347472-n6811ens	31	8	the	the	DT
cord-347472-n6811ens	31	9	side	side	NN
cord-347472-n6811ens	31	10	of	of	IN
cord-347472-n6811ens	31	11	caution	caution	NN
cord-347472-n6811ens	31	12	and	and	CC
cord-347472-n6811ens	31	13	re	re	NN
cord-347472-n6811ens	31	14	-	-	NN
cord-347472-n6811ens	31	15	test	test	NN
cord-347472-n6811ens	31	16	if	if	IN
cord-347472-n6811ens	31	17	concerned	concern	VBN
cord-347472-n6811ens	31	18	.	.	.
cord-347472-n6811ens	32	1	Three	three	CD
cord-347472-n6811ens	32	2	,	,	,
cord-347472-n6811ens	32	3	the	the	DT
cord-347472-n6811ens	32	4	FDA	FDA	NNP
cord-347472-n6811ens	32	5	had	have	VBD
cord-347472-n6811ens	32	6	approved	approve	VBN
cord-347472-n6811ens	32	7	this	this	DT
cord-347472-n6811ens	32	8	test	test	NN
cord-347472-n6811ens	32	9	,	,	,
cord-347472-n6811ens	32	10	making	make	VBG
cord-347472-n6811ens	32	11	it	-PRON-	PRP
cord-347472-n6811ens	32	12	,	,	,
cord-347472-n6811ens	32	13	de	de	FW
cord-347472-n6811ens	32	14	jure	jure	NNP
cord-347472-n6811ens	32	15	correct	correct	JJ
cord-347472-n6811ens	32	16	(	(	-LRB-
cord-347472-n6811ens	32	17	full	full	JJ
cord-347472-n6811ens	32	18	correspondence	correspondence	NN
cord-347472-n6811ens	32	19	in	in	IN
cord-347472-n6811ens	32	20	Supplemental	Supplemental	NNP
cord-347472-n6811ens	32	21	Materials	Materials	NNP
cord-347472-n6811ens	32	22	)	)	-RRB-
cord-347472-n6811ens	32	23	.	.	.
cord-347472-n6811ens	33	1	False	false	JJ
cord-347472-n6811ens	33	2	negatives	negative	NNS
cord-347472-n6811ens	33	3	are	be	VBP
cord-347472-n6811ens	33	4	inherently	inherently	RB
cord-347472-n6811ens	33	5	difficult	difficult	JJ
cord-347472-n6811ens	33	6	to	to	TO
cord-347472-n6811ens	33	7	detect	detect	VB
cord-347472-n6811ens	33	8	-particularly	-particularly	RB
cord-347472-n6811ens	33	9	when	when	WRB
cord-347472-n6811ens	33	10	the	the	DT
cord-347472-n6811ens	33	11	assay	assay	NN
cord-347472-n6811ens	33	12	in	in	IN
cord-347472-n6811ens	33	13	question	question	NN
cord-347472-n6811ens	33	14	is	be	VBZ
cord-347472-n6811ens	33	15	the	the	DT
cord-347472-n6811ens	33	16	current	current	JJ
cord-347472-n6811ens	33	17	gold	gold	NN
cord-347472-n6811ens	33	18	standard	standard	NN
cord-347472-n6811ens	33	19	.	.	.
cord-347472-n6811ens	34	1	Testing	testing	NN
cord-347472-n6811ens	34	2	reagents	reagent	NNS
cord-347472-n6811ens	34	3	are	be	VBP
cord-347472-n6811ens	34	4	in	in	IN
cord-347472-n6811ens	34	5	short	short	JJ
cord-347472-n6811ens	34	6	supply	supply	NN
cord-347472-n6811ens	34	7	,	,	,
cord-347472-n6811ens	34	8	making	make	VBG
cord-347472-n6811ens	34	9	it	-PRON-	PRP
cord-347472-n6811ens	34	10	hard	hard	JJ
cord-347472-n6811ens	34	11	for	for	IN
cord-347472-n6811ens	34	12	individuals	individual	NNS
cord-347472-n6811ens	34	13	to	to	TO
cord-347472-n6811ens	34	14	be	be	VB
cord-347472-n6811ens	34	15	tested	test	VBN
cord-347472-n6811ens	34	16	once	once	RB
cord-347472-n6811ens	34	17	,	,	,
cord-347472-n6811ens	34	18	much	much	RB
cord-347472-n6811ens	34	19	less	less	RBR
cord-347472-n6811ens	34	20	repeatedly	repeatedly	RB
cord-347472-n6811ens	34	21	.	.	.
cord-347472-n6811ens	35	1	Official	official	JJ
cord-347472-n6811ens	35	2	approval	approval	NN
cord-347472-n6811ens	35	3	is	be	VBZ
cord-347472-n6811ens	35	4	not	not	RB
cord-347472-n6811ens	35	5	a	a	DT
cord-347472-n6811ens	35	6	defense	defense	NN
cord-347472-n6811ens	35	7	against	against	IN
cord-347472-n6811ens	35	8	facts	fact	NNS
cord-347472-n6811ens	35	9	.	.	.
cord-347472-n6811ens	36	1	As	as	IN
cord-347472-n6811ens	36	2	I	-PRON-	PRP
cord-347472-n6811ens	36	3	show	show	VBP
cord-347472-n6811ens	36	4	below	below	IN
cord-347472-n6811ens	36	5	,	,	,
cord-347472-n6811ens	36	6	the	the	DT
cord-347472-n6811ens	36	7	CDC	CDC	NNP
cord-347472-n6811ens	36	8	design	design	NN
cord-347472-n6811ens	36	9	is	be	VBZ
cord-347472-n6811ens	36	10	both	both	DT
cord-347472-n6811ens	36	11	flawed	flawed	JJ
cord-347472-n6811ens	36	12	and	and	CC
cord-347472-n6811ens	36	13	fixable	fixable	JJ
cord-347472-n6811ens	36	14	.	.	.
cord-347472-n6811ens	37	1	Individuals	individual	NNS
cord-347472-n6811ens	37	2	would	would	MD
cord-347472-n6811ens	37	3	be	be	VB
cord-347472-n6811ens	37	4	incensed	incense	VBN
cord-347472-n6811ens	37	5	by	by	IN
cord-347472-n6811ens	37	6	a	a	DT
cord-347472-n6811ens	37	7	preventable	preventable	JJ
cord-347472-n6811ens	37	8	false	false	JJ
cord-347472-n6811ens	37	9	-	-	HYPH
cord-347472-n6811ens	37	10	positive	positive	JJ
cord-347472-n6811ens	37	11	diagnosis	diagnosis	NN
cord-347472-n6811ens	37	12	putting	put	VBG
cord-347472-n6811ens	37	13	them	-PRON-	PRP
cord-347472-n6811ens	37	14	into	into	IN
cord-347472-n6811ens	37	15	unnecessary	unnecessary	JJ
cord-347472-n6811ens	37	16	quarantine	quarantine	NN
cord-347472-n6811ens	37	17	.	.	.
cord-347472-n6811ens	38	1	The	the	DT
cord-347472-n6811ens	38	2	public	public	NN
cord-347472-n6811ens	38	3	should	should	MD
cord-347472-n6811ens	38	4	be	be	VB
cord-347472-n6811ens	38	5	incensed	incense	VBN
cord-347472-n6811ens	38	6	by	by	IN
cord-347472-n6811ens	38	7	a	a	DT
cord-347472-n6811ens	38	8	poorly	poorly	RB
cord-347472-n6811ens	38	9	designed	design	VBN
cord-347472-n6811ens	38	10	official	official	JJ
cord-347472-n6811ens	38	11	assay	assay	NN
cord-347472-n6811ens	38	12	whose	whose	WP$
cord-347472-n6811ens	38	13	preventable	preventable	JJ
cord-347472-n6811ens	38	14	false	false	JJ
cord-347472-n6811ens	38	15	-	-	HYPH
cord-347472-n6811ens	38	16	negative	negative	JJ
cord-347472-n6811ens	38	17	diagnoses	diagnosis	NNS
cord-347472-n6811ens	38	18	allow	allow	VBP
cord-347472-n6811ens	38	19	virus	virus	NN
cord-347472-n6811ens	38	20	-	-	HYPH
cord-347472-n6811ens	38	21	positive	positive	JJ
cord-347472-n6811ens	38	22	patients	patient	NNS
cord-347472-n6811ens	38	23	back	back	RB
cord-347472-n6811ens	38	24	into	into	IN
cord-347472-n6811ens	38	25	society	society	NN
cord-347472-n6811ens	38	26	.	.	.
cord-347472-n6811ens	39	1	The	the	DT
cord-347472-n6811ens	39	2	2019-nCoV	2019-ncov	CD
cord-347472-n6811ens	39	3	Real	Real	NNP
cord-347472-n6811ens	39	4	-	-	HYPH
cord-347472-n6811ens	39	5	Time	time	NN
cord-347472-n6811ens	39	6	RT	RT	NNP
cord-347472-n6811ens	39	7	-	-	HYPH
cord-347472-n6811ens	39	8	PCR	PCR	NNP
cord-347472-n6811ens	39	9	Diagnostic	Diagnostic	NNP
cord-347472-n6811ens	39	10	Panel	Panel	NNP
cord-347472-n6811ens	39	11	is	be	VBZ
cord-347472-n6811ens	39	12	a	a	DT
cord-347472-n6811ens	39	13	CDC	CDC	NNP
cord-347472-n6811ens	39	14	-	-	HYPH
cord-347472-n6811ens	39	15	designed	design	VBN
cord-347472-n6811ens	39	16	and	and	CC
cord-347472-n6811ens	39	17	validated	validate	VBD
cord-347472-n6811ens	39	18	COVID-19	COVID-19	NNP
cord-347472-n6811ens	39	19	assay	assay	NN
cord-347472-n6811ens	39	20	comprised	comprise	VBN
cord-347472-n6811ens	39	21	of	of	IN
cord-347472-n6811ens	39	22	three	three	CD
cord-347472-n6811ens	39	23	qPCR	qPCR	NNP
cord-347472-n6811ens	39	24	primer	primer	NN
cord-347472-n6811ens	39	25	/	/	SYM
cord-347472-n6811ens	39	26	probe	probe	NN
cord-347472-n6811ens	39	27	sets	set	NNS
cord-347472-n6811ens	39	28	.	.	.
cord-347472-n6811ens	40	1	N1	N1	NNP
cord-347472-n6811ens	40	2	and	and	CC
cord-347472-n6811ens	40	3	N2	N2	NNP
cord-347472-n6811ens	40	4	generate	generate	VB
cord-347472-n6811ens	40	5	SARS	SARS	NNP
cord-347472-n6811ens	40	6	-	-	HYPH
cord-347472-n6811ens	40	7	CoV-2-specific	CoV-2-specific	NNP
cord-347472-n6811ens	40	8	amplicons	amplicon	NNS
cord-347472-n6811ens	40	9	from	from	IN
cord-347472-n6811ens	40	10	reverse	reverse	NN
cord-347472-n6811ens	40	11	transcribed	transcribe	VBN
cord-347472-n6811ens	40	12	viral	viral	JJ
cord-347472-n6811ens	40	13	RNA	RNA	NNP
cord-347472-n6811ens	40	14	.	.	.
cord-347472-n6811ens	41	1	The	the	DT
cord-347472-n6811ens	41	2	third	third	JJ
cord-347472-n6811ens	41	3	,	,	,
cord-347472-n6811ens	41	4	RP	RP	NNP
cord-347472-n6811ens	41	5	,	,	,
cord-347472-n6811ens	41	6	targets	target	VBZ
cord-347472-n6811ens	41	7	the	the	DT
cord-347472-n6811ens	41	8	human	human	NN
cord-347472-n6811ens	41	9	RPP30	rpp30	IN
cord-347472-n6811ens	41	10	gene	gene	NN
cord-347472-n6811ens	41	11	and	and	CC
cord-347472-n6811ens	41	12	is	be	VBZ
cord-347472-n6811ens	41	13	an	an	DT
cord-347472-n6811ens	41	14	"	"	``
cord-347472-n6811ens	41	15	extraction	extraction	NN
cord-347472-n6811ens	41	16	control	control	NN
cord-347472-n6811ens	41	17	"	"	''
cord-347472-n6811ens	41	18	that	that	WDT
cord-347472-n6811ens	41	19	is	be	VBZ
cord-347472-n6811ens	41	20	intended	intend	VBN
cord-347472-n6811ens	41	21	and	and	CC
cord-347472-n6811ens	41	22	claimed	claim	VBN
cord-347472-n6811ens	41	23	to	to	TO
cord-347472-n6811ens	41	24	inform	inform	VB
cord-347472-n6811ens	41	25	about	about	IN
cord-347472-n6811ens	41	26	successful	successful	JJ
cord-347472-n6811ens	41	27	sample	sample	NN
cord-347472-n6811ens	41	28	collection	collection	NN
cord-347472-n6811ens	41	29	,	,	,
cord-347472-n6811ens	41	30	RNA	RNA	NNP
cord-347472-n6811ens	41	31	extraction	extraction	NN
cord-347472-n6811ens	41	32	,	,	,
cord-347472-n6811ens	41	33	and	and	CC
cord-347472-n6811ens	41	34	reverse	reverse	VB
cord-347472-n6811ens	41	35	transcription	transcription	NN
cord-347472-n6811ens	41	36	(	(	-LRB-
cord-347472-n6811ens	41	37	3	3	CD
cord-347472-n6811ens	41	38	)	)	-RRB-
cord-347472-n6811ens	41	39	.	.	.
cord-347472-n6811ens	42	1	It	-PRON-	PRP
cord-347472-n6811ens	42	2	does	do	VBZ
cord-347472-n6811ens	42	3	not	not	RB
cord-347472-n6811ens	42	4	perform	perform	VB
cord-347472-n6811ens	42	5	these	these	DT
cord-347472-n6811ens	42	6	functions	function	NNS
cord-347472-n6811ens	42	7	in	in	IN
cord-347472-n6811ens	42	8	practice	practice	NN
cord-347472-n6811ens	42	9	.	.	.
cord-347472-n6811ens	43	1	The	the	DT
cord-347472-n6811ens	43	2	CDC	CDC	NNP
cord-347472-n6811ens	43	3	design	design	NN
cord-347472-n6811ens	43	4	is	be	VBZ
cord-347472-n6811ens	43	5	fundamentally	fundamentally	RB
cord-347472-n6811ens	43	6	flawed	flawed	JJ
cord-347472-n6811ens	43	7	and	and	CC
cord-347472-n6811ens	43	8	has	have	VBZ
cord-347472-n6811ens	43	9	the	the	DT
cord-347472-n6811ens	43	10	potential	potential	NN
cord-347472-n6811ens	43	11	to	to	TO
cord-347472-n6811ens	43	12	return	return	VB
cord-347472-n6811ens	43	13	dangerous	dangerous	JJ
cord-347472-n6811ens	43	14	false	false	JJ
cord-347472-n6811ens	43	15	negatives	negative	NNS
cord-347472-n6811ens	43	16	(	(	-LRB-
cord-347472-n6811ens	43	17	Fig	Fig	NNP
cord-347472-n6811ens	43	18	.	.	.
cord-347472-n6811ens	43	19	1A	1a	NN
cord-347472-n6811ens	43	20	)	)	-RRB-
cord-347472-n6811ens	43	21	.	.	.
cord-347472-n6811ens	44	1	The	the	DT
cord-347472-n6811ens	44	2	CDC	CDC	NNP
cord-347472-n6811ens	44	3	-	-	HYPH
cord-347472-n6811ens	44	4	specified	specify	VBN
cord-347472-n6811ens	44	5	RP	rp	NN
cord-347472-n6811ens	44	6	extraction	extraction	NN
cord-347472-n6811ens	44	7	control	control	NN
cord-347472-n6811ens	44	8	design	design	NN
cord-347472-n6811ens	44	9	generates	generate	VBZ
cord-347472-n6811ens	44	10	identical	identical	JJ
cord-347472-n6811ens	44	11	amplicons	amplicon	NNS
cord-347472-n6811ens	44	12	from	from	IN
cord-347472-n6811ens	44	13	reverse	reverse	NN
cord-347472-n6811ens	44	14	transcribed	transcribe	VBN
cord-347472-n6811ens	44	15	RNA	RNA	NNP
cord-347472-n6811ens	44	16	and	and	CC
cord-347472-n6811ens	44	17	human	human	JJ
cord-347472-n6811ens	44	18	genomic	genomic	JJ
cord-347472-n6811ens	44	19	DNA	dna	NN
cord-347472-n6811ens	44	20	.	.	.
cord-347472-n6811ens	45	1	Megablast	Megablast	NNP
cord-347472-n6811ens	45	2	(	(	-LRB-
cord-347472-n6811ens	45	3	4	4	LS
cord-347472-n6811ens	45	4	)	)	-RRB-
cord-347472-n6811ens	45	5	search	search	NN
cord-347472-n6811ens	45	6	of	of	IN
cord-347472-n6811ens	45	7	the	the	DT
cord-347472-n6811ens	45	8	RP	RP	NNP
cord-347472-n6811ens	45	9	forward	forward	RB
cord-347472-n6811ens	45	10	,	,	,
cord-347472-n6811ens	45	11	reverse	reverse	JJ
cord-347472-n6811ens	45	12	,	,	,
cord-347472-n6811ens	45	13	and	and	CC
cord-347472-n6811ens	45	14	probe	probe	VB
cord-347472-n6811ens	45	15	sequences	sequence	NNS
cord-347472-n6811ens	45	16	against	against	IN
cord-347472-n6811ens	45	17	the	the	DT
cord-347472-n6811ens	45	18	human	human	JJ
cord-347472-n6811ens	45	19	refseq_rna	refseq_rna	NNP
cord-347472-n6811ens	45	20	transcript	transcript	NN
cord-347472-n6811ens	45	21	database	database	NN
cord-347472-n6811ens	45	22	identifies	identify	VBZ
cord-347472-n6811ens	45	23	perfect	perfect	JJ
cord-347472-n6811ens	45	24	matches	match	NNS
cord-347472-n6811ens	45	25	within	within	IN
cord-347472-n6811ens	45	26	the	the	DT
cord-347472-n6811ens	45	27	RPP30	RPP30	NNP
cord-347472-n6811ens	45	28	mRNA	mrna	NN
cord-347472-n6811ens	45	29	.	.	.
cord-347472-n6811ens	46	1	The	the	DT
cord-347472-n6811ens	46	2	design	design	NN
cord-347472-n6811ens	46	3	recognizes	recognize	VBZ
cord-347472-n6811ens	46	4	sequences	sequence	NNS
cord-347472-n6811ens	46	5	that	that	WDT
cord-347472-n6811ens	46	6	are	be	VBP
cord-347472-n6811ens	46	7	entirely	entirely	RB
cord-347472-n6811ens	46	8	contained	contain	VBN
cord-347472-n6811ens	46	9	within	within	IN
cord-347472-n6811ens	46	10	a	a	DT
cord-347472-n6811ens	46	11	single	single	JJ
cord-347472-n6811ens	46	12	exon	exon	NN
cord-347472-n6811ens	46	13	,	,	,
cord-347472-n6811ens	46	14	and	and	CC
cord-347472-n6811ens	46	15	therefore	therefore	RB
cord-347472-n6811ens	46	16	generates	generate	VBZ
cord-347472-n6811ens	46	17	an	an	DT
cord-347472-n6811ens	46	18	identical	identical	JJ
cord-347472-n6811ens	46	19	amplicon	amplicon	NN
cord-347472-n6811ens	46	20	from	from	IN
cord-347472-n6811ens	46	21	the	the	DT
cord-347472-n6811ens	46	22	RPP30	RPP30	NNP
cord-347472-n6811ens	46	23	genomic	genomic	JJ
cord-347472-n6811ens	46	24	locus	locus	NN
cord-347472-n6811ens	46	25	(	(	-LRB-
cord-347472-n6811ens	46	26	Fig	Fig	NNP
cord-347472-n6811ens	46	27	.	.	.
cord-347472-n6811ens	46	28	 	 	_SP
cord-347472-n6811ens	46	29	1B	1b	CD
cord-347472-n6811ens	46	30	)	)	-RRB-
cord-347472-n6811ens	46	31	.	.	.
cord-347472-n6811ens	47	1	The	the	DT
cord-347472-n6811ens	47	2	CDC	CDC	NNP
cord-347472-n6811ens	47	3	extraction	extraction	NN
cord-347472-n6811ens	47	4	control	control	NN
cord-347472-n6811ens	47	5	generates	generate	VBZ
cord-347472-n6811ens	47	6	a	a	DT
cord-347472-n6811ens	47	7	positive	positive	JJ
cord-347472-n6811ens	47	8	signal	signal	NN
cord-347472-n6811ens	47	9	in	in	IN
cord-347472-n6811ens	47	10	reactions	reaction	NNS
cord-347472-n6811ens	47	11	containing	contain	VBG
cord-347472-n6811ens	47	12	intact	intact	JJ
cord-347472-n6811ens	47	13	RNA	RNA	NNP
cord-347472-n6811ens	47	14	.	.	.
cord-347472-n6811ens	48	1	It	-PRON-	PRP
cord-347472-n6811ens	48	2	also	also	RB
cord-347472-n6811ens	48	3	generates	generate	VBZ
cord-347472-n6811ens	48	4	a	a	DT
cord-347472-n6811ens	48	5	positive	positive	JJ
cord-347472-n6811ens	48	6	signal	signal	NN
cord-347472-n6811ens	48	7	from	from	IN
cord-347472-n6811ens	48	8	RNA	rna	NN
cord-347472-n6811ens	48	9	-	-	HYPH
cord-347472-n6811ens	48	10	free	free	JJ
cord-347472-n6811ens	48	11	reactions	reaction	NNS
cord-347472-n6811ens	48	12	containing	contain	VBG
cord-347472-n6811ens	48	13	human	human	JJ
cord-347472-n6811ens	48	14	genomic	genomic	JJ
cord-347472-n6811ens	48	15	DNA	dna	NN
cord-347472-n6811ens	48	16	(	(	-LRB-
cord-347472-n6811ens	48	17	Fig	Fig	NNP
cord-347472-n6811ens	48	18	.	.	.
cord-347472-n6811ens	48	19	S1	S1	NNP
cord-347472-n6811ens	48	20	)	)	-RRB-
cord-347472-n6811ens	48	21	.	.	.
cord-347472-n6811ens	49	1	Genomic	genomic	JJ
cord-347472-n6811ens	49	2	DNA	dna	NN
cord-347472-n6811ens	49	3	is	be	VBZ
cord-347472-n6811ens	49	4	co	co	VBN
cord-347472-n6811ens	49	5	-	-	JJ
cord-347472-n6811ens	49	6	purified	purify	VBN
cord-347472-n6811ens	49	7	in	in	IN
cord-347472-n6811ens	49	8	quantities	quantity	NNS
cord-347472-n6811ens	49	9	sufficient	sufficient	JJ
cord-347472-n6811ens	49	10	to	to	TO
cord-347472-n6811ens	49	11	generate	generate	VB
cord-347472-n6811ens	49	12	strong	strong	JJ
cord-347472-n6811ens	49	13	positive	positive	JJ
cord-347472-n6811ens	49	14	signals	signal	NNS
cord-347472-n6811ens	49	15	for	for	IN
cord-347472-n6811ens	49	16	the	the	DT
cord-347472-n6811ens	49	17	CDC	CDC	NNP
cord-347472-n6811ens	49	18	-	-	HYPH
cord-347472-n6811ens	49	19	specified	specify	VBN
cord-347472-n6811ens	49	20	extraction	extraction	NN
cord-347472-n6811ens	49	21	control	control	NN
cord-347472-n6811ens	49	22	during	during	IN
cord-347472-n6811ens	49	23	work	work	NN
cord-347472-n6811ens	49	24	-	-	HYPH
cord-347472-n6811ens	49	25	up	up	NN
cord-347472-n6811ens	49	26	of	of	IN
cord-347472-n6811ens	49	27	clinical	clinical	JJ
cord-347472-n6811ens	49	28	RNA	RNA	NNP
cord-347472-n6811ens	49	29	specimens	specimen	NNS
cord-347472-n6811ens	49	30	.	.	.
cord-347472-n6811ens	50	1	To	to	TO
cord-347472-n6811ens	50	2	test	test	VB
cord-347472-n6811ens	50	3	for	for	IN
cord-347472-n6811ens	50	4	the	the	DT
cord-347472-n6811ens	50	5	presence	presence	NN
cord-347472-n6811ens	50	6	of	of	IN
cord-347472-n6811ens	50	7	control	control	NN
cord-347472-n6811ens	50	8	-	-	HYPH
cord-347472-n6811ens	50	9	affecting	affect	VBG
cord-347472-n6811ens	50	10	DNA	dna	NN
cord-347472-n6811ens	50	11	,	,	,
cord-347472-n6811ens	50	12	qPCR	qpcr	JJ
cord-347472-n6811ens	50	13	reactions	reaction	NNS
cord-347472-n6811ens	50	14	lacking	lack	VBG
cord-347472-n6811ens	51	1	reverse	reverse	JJ
cord-347472-n6811ens	51	2	transcriptase	transcriptase	NNP
cord-347472-n6811ens	51	3	were	be	VBD
cord-347472-n6811ens	51	4	performed	perform	VBN
cord-347472-n6811ens	51	5	on	on	IN
cord-347472-n6811ens	51	6	SARS	SARS	NNP
cord-347472-n6811ens	51	7	-	-	HYPH
cord-347472-n6811ens	51	8	CoV-2-positive	cov-2-positive	JJ
cord-347472-n6811ens	51	9	clinical	clinical	JJ
cord-347472-n6811ens	51	10	samples	sample	NNS
cord-347472-n6811ens	51	11	using	use	VBG
cord-347472-n6811ens	51	12	the	the	DT
cord-347472-n6811ens	51	13	CDC	CDC	NNP
cord-347472-n6811ens	51	14	-	-	HYPH
cord-347472-n6811ens	51	15	specified	specify	VBN
cord-347472-n6811ens	51	16	RP	RP	NNP
cord-347472-n6811ens	51	17	primer	primer	NN
cord-347472-n6811ens	51	18	and	and	CC
cord-347472-n6811ens	51	19	probe	probe	NN
cord-347472-n6811ens	51	20	.	.	.
cord-347472-n6811ens	51	21	"	"	''
cord-347472-n6811ens	52	1	Purified	Purified	NNP
cord-347472-n6811ens	52	2	RNA	RNA	NNP
cord-347472-n6811ens	52	3	"	"	''
cord-347472-n6811ens	52	4	samples	sample	NNS
cord-347472-n6811ens	52	5	from	from	IN
cord-347472-n6811ens	52	6	COVID-19	covid-19	JJ
cord-347472-n6811ens	52	7	cases	case	NNS
cord-347472-n6811ens	52	8	were	be	VBD
cord-347472-n6811ens	52	9	obtained	obtain	VBN
cord-347472-n6811ens	52	10	from	from	IN
cord-347472-n6811ens	52	11	a	a	DT
cord-347472-n6811ens	52	12	CAP	cap	NN
cord-347472-n6811ens	52	13	/	/	SYM
cord-347472-n6811ens	52	14	CLIA	CLIA	NNP
cord-347472-n6811ens	52	15	certified	certify	VBN
cord-347472-n6811ens	52	16	university	university	NN
cord-347472-n6811ens	52	17	hospital	hospital	NN
cord-347472-n6811ens	52	18	molecular	molecular	JJ
cord-347472-n6811ens	52	19	pathology	pathology	NN
cord-347472-n6811ens	52	20	laboratory	laboratory	NN
cord-347472-n6811ens	52	21	.	.	.
cord-347472-n6811ens	53	1	Samples	sample	NNS
cord-347472-n6811ens	53	2	had	have	VBD
cord-347472-n6811ens	53	3	been	be	VBN
cord-347472-n6811ens	53	4	processed	process	VBN
cord-347472-n6811ens	53	5	using	use	VBG
cord-347472-n6811ens	53	6	either	either	CC
cord-347472-n6811ens	53	7	solid	solid	JJ
cord-347472-n6811ens	53	8	phase	phase	NN
cord-347472-n6811ens	53	9	extraction	extraction	NN
cord-347472-n6811ens	53	10	(	(	-LRB-
cord-347472-n6811ens	53	11	Qiagen	Qiagen	NNP
cord-347472-n6811ens	53	12	)	)	-RRB-
cord-347472-n6811ens	53	13	or	or	CC
cord-347472-n6811ens	53	14	low	low	JJ
cord-347472-n6811ens	53	15	pH	pH	NNP
cord-347472-n6811ens	53	16	organic	organic	JJ
cord-347472-n6811ens	53	17	extraction	extraction	NN
cord-347472-n6811ens	53	18	(	(	-LRB-
cord-347472-n6811ens	53	19	Trizol	Trizol	NNP
cord-347472-n6811ens	53	20	)	)	-RRB-
cord-347472-n6811ens	53	21	.	.	.
cord-347472-n6811ens	54	1	Both	both	DT
cord-347472-n6811ens	54	2	methods	method	NNS
cord-347472-n6811ens	54	3	are	be	VBP
cord-347472-n6811ens	54	4	permitted	permit	VBN
cord-347472-n6811ens	54	5	under	under	IN
cord-347472-n6811ens	54	6	current	current	JJ
cord-347472-n6811ens	54	7	Emergency	Emergency	NNP
cord-347472-n6811ens	54	8	Use	Use	NNP
cord-347472-n6811ens	54	9	Authorization	Authorization	NNP
cord-347472-n6811ens	54	10	(	(	-LRB-
cord-347472-n6811ens	54	11	EUA	EUA	NNP
cord-347472-n6811ens	54	12	)	)	-RRB-
cord-347472-n6811ens	54	13	for	for	IN
cord-347472-n6811ens	54	14	RNA	rna	NN
cord-347472-n6811ens	54	15	isolation	isolation	NN
cord-347472-n6811ens	54	16	.	.	.
cord-347472-n6811ens	55	1	In	in	IN
cord-347472-n6811ens	55	2	reality	reality	NN
cord-347472-n6811ens	55	3	,	,	,
cord-347472-n6811ens	55	4	both	both	DT
cord-347472-n6811ens	55	5	methods	method	NNS
cord-347472-n6811ens	55	6	co	co	VBP
cord-347472-n6811ens	55	7	-	-	VB
cord-347472-n6811ens	55	8	purify	purify	VB
cord-347472-n6811ens	55	9	significant	significant	JJ
cord-347472-n6811ens	55	10	amounts	amount	NNS
cord-347472-n6811ens	55	11	of	of	IN
cord-347472-n6811ens	55	12	DNA	dna	NN
cord-347472-n6811ens	55	13	(	(	-LRB-
cord-347472-n6811ens	55	14	5	5	CD
cord-347472-n6811ens	55	15	)	)	-RRB-
cord-347472-n6811ens	55	16	.	.	.
cord-347472-n6811ens	56	1	All	all	DT
cord-347472-n6811ens	56	2	clinical	clinical	JJ
cord-347472-n6811ens	56	3	samples	sample	NNS
cord-347472-n6811ens	56	4	tested	test	VBN
cord-347472-n6811ens	56	5	generated	generate	VBD
cord-347472-n6811ens	56	6	unambiguous	unambiguous	JJ
cord-347472-n6811ens	56	7	extraction	extraction	NN
cord-347472-n6811ens	56	8	control	control	NN
cord-347472-n6811ens	56	9	positive	positive	JJ
cord-347472-n6811ens	56	10	signals	signal	NNS
cord-347472-n6811ens	56	11	in	in	IN
cord-347472-n6811ens	56	12	the	the	DT
cord-347472-n6811ens	56	13	absence	absence	NN
cord-347472-n6811ens	56	14	of	of	IN
cord-347472-n6811ens	56	15	reverse	reverse	JJ
cord-347472-n6811ens	56	16	transcription	transcription	NN
cord-347472-n6811ens	56	17	,	,	,
cord-347472-n6811ens	56	18	a	a	DT
cord-347472-n6811ens	56	19	reaction	reaction	NN
cord-347472-n6811ens	56	20	context	context	NN
cord-347472-n6811ens	56	21	that	that	WDT
cord-347472-n6811ens	56	22	could	could	MD
cord-347472-n6811ens	56	23	not	not	RB
cord-347472-n6811ens	56	24	have	have	VB
cord-347472-n6811ens	56	25	detected	detect	VBN
cord-347472-n6811ens	56	26	virus	virus	NN
cord-347472-n6811ens	56	27	an	an	DT
cord-347472-n6811ens	56	28	RNA	RNA	NNP
cord-347472-n6811ens	56	29	virus	virus	NN
cord-347472-n6811ens	56	30	(	(	-LRB-
cord-347472-n6811ens	56	31	Fig	Fig	NNP
cord-347472-n6811ens	56	32	.	.	.
cord-347472-n6811ens	56	33	2A	2a	CD
cord-347472-n6811ens	56	34	)	)	-RRB-
cord-347472-n6811ens	57	1	Single	single	JJ
cord-347472-n6811ens	57	2	-	-	HYPH
cord-347472-n6811ens	57	3	digit	digit	NN
cord-347472-n6811ens	57	4	copies	copy	NNS
cord-347472-n6811ens	57	5	of	of	IN
cord-347472-n6811ens	57	6	genomic	genomic	JJ
cord-347472-n6811ens	57	7	DNA	dna	NN
cord-347472-n6811ens	57	8	are	be	VBP
cord-347472-n6811ens	57	9	sufficient	sufficient	JJ
cord-347472-n6811ens	57	10	to	to	TO
cord-347472-n6811ens	57	11	generate	generate	VB
cord-347472-n6811ens	57	12	a	a	DT
cord-347472-n6811ens	57	13	positive	positive	JJ
cord-347472-n6811ens	57	14	control	control	NN
cord-347472-n6811ens	57	15	signal	signal	NN
cord-347472-n6811ens	57	16	using	use	VBG
cord-347472-n6811ens	57	17	the	the	DT
cord-347472-n6811ens	57	18	CDC	CDC	NNP
cord-347472-n6811ens	57	19	-	-	HYPH
cord-347472-n6811ens	57	20	designed	design	VBN
cord-347472-n6811ens	57	21	assay	assay	NN
cord-347472-n6811ens	57	22	.	.	.
cord-347472-n6811ens	58	1	Driven	drive	VBN
cord-347472-n6811ens	58	2	by	by	IN
cord-347472-n6811ens	58	3	concern	concern	NN
cord-347472-n6811ens	58	4	about	about	IN
cord-347472-n6811ens	58	5	the	the	DT
cord-347472-n6811ens	58	6	control	control	NN
cord-347472-n6811ens	58	7	probe	probe	NN
cord-347472-n6811ens	58	8	signals	signal	NNS
cord-347472-n6811ens	58	9	observed	observe	VBN
cord-347472-n6811ens	58	10	in	in	IN
cord-347472-n6811ens	58	11	PCR	PCR	NNP
cord-347472-n6811ens	58	12	-	-	HYPH
cord-347472-n6811ens	58	13	only	only	JJ
cord-347472-n6811ens	58	14	analysis	analysis	NN
cord-347472-n6811ens	58	15	,	,	,
cord-347472-n6811ens	58	16	I	-PRON-	PRP
cord-347472-n6811ens	58	17	determined	determine	VBD
cord-347472-n6811ens	58	18	the	the	DT
cord-347472-n6811ens	58	19	quantity	quantity	NN
cord-347472-n6811ens	58	20	of	of	IN
cord-347472-n6811ens	58	21	genomic	genomic	JJ
cord-347472-n6811ens	58	22	DNA	dna	NN
cord-347472-n6811ens	58	23	required	require	VBN
cord-347472-n6811ens	58	24	to	to	TO
cord-347472-n6811ens	58	25	generate	generate	VB
cord-347472-n6811ens	58	26	an	an	DT
cord-347472-n6811ens	58	27	assay	assay	NN
cord-347472-n6811ens	58	28	-	-	HYPH
cord-347472-n6811ens	58	29	positive	positive	JJ
cord-347472-n6811ens	58	30	control	control	NN
cord-347472-n6811ens	58	31	signal	signal	NN
cord-347472-n6811ens	58	32	.	.	.
cord-347472-n6811ens	59	1	Commercially	commercially	RB
cord-347472-n6811ens	59	2	supplied	supply	VBN
cord-347472-n6811ens	59	3	,	,	,
cord-347472-n6811ens	59	4	pooled	pool	VBN
cord-347472-n6811ens	59	5	human	human	JJ
cord-347472-n6811ens	59	6	DNA	dna	NN
cord-347472-n6811ens	59	7	was	be	VBD
cord-347472-n6811ens	59	8	serially	serially	RB
cord-347472-n6811ens	59	9	diluted	dilute	VBN
cord-347472-n6811ens	59	10	across	across	IN
cord-347472-n6811ens	59	11	several	several	JJ
cord-347472-n6811ens	59	12	orders	order	NNS
cord-347472-n6811ens	59	13	of	of	IN
cord-347472-n6811ens	59	14	magnitude	magnitude	NN
cord-347472-n6811ens	59	15	and	and	CC
cord-347472-n6811ens	59	16	analyzed	analyze	VBN
cord-347472-n6811ens	59	17	by	by	IN
cord-347472-n6811ens	59	18	the	the	DT
cord-347472-n6811ens	59	19	same	same	JJ
cord-347472-n6811ens	59	20	one	one	CD
cord-347472-n6811ens	59	21	-	-	HYPH
cord-347472-n6811ens	59	22	step	step	NN
cord-347472-n6811ens	59	23	RT	RT	NNP
cord-347472-n6811ens	59	24	-	-	HYPH
cord-347472-n6811ens	59	25	qPCR	qPCR	NNP
cord-347472-n6811ens	59	26	used	use	VBN
cord-347472-n6811ens	59	27	for	for	IN
cord-347472-n6811ens	59	28	clinical	clinical	JJ
cord-347472-n6811ens	59	29	samples	sample	NNS
cord-347472-n6811ens	59	30	(	(	-LRB-
cord-347472-n6811ens	59	31	Fig	Fig	NNP
cord-347472-n6811ens	59	32	.	.	.
cord-347472-n6811ens	59	33	 	 	_SP
cord-347472-n6811ens	59	34	2B	2b	CD
cord-347472-n6811ens	59	35	)	)	-RRB-
cord-347472-n6811ens	59	36	.	.	.
cord-347472-n6811ens	60	1	Extrapolating	extrapolate	VBG
cord-347472-n6811ens	60	2	from	from	IN
cord-347472-n6811ens	60	3	pure	pure	JJ
cord-347472-n6811ens	60	4	-	-	HYPH
cord-347472-n6811ens	60	5	DNA	dna	NN
cord-347472-n6811ens	60	6	samples	sample	NNS
cord-347472-n6811ens	60	7	,	,	,
cord-347472-n6811ens	60	8	a	a	DT
cord-347472-n6811ens	60	9	positive	positive	JJ
cord-347472-n6811ens	60	10	signal	signal	NN
cord-347472-n6811ens	60	11	from	from	IN
cord-347472-n6811ens	60	12	the	the	DT
cord-347472-n6811ens	60	13	CDC	CDC	NNP
cord-347472-n6811ens	60	14	control	control	NN
cord-347472-n6811ens	60	15	would	would	MD
cord-347472-n6811ens	60	16	be	be	VB
cord-347472-n6811ens	60	17	readily	readily	RB
cord-347472-n6811ens	60	18	achieved	achieve	VBN
cord-347472-n6811ens	60	19	in	in	IN
cord-347472-n6811ens	60	20	samples	sample	NNS
cord-347472-n6811ens	60	21	containing	contain	VBG
cord-347472-n6811ens	60	22	one	one	CD
cord-347472-n6811ens	60	23	copy	copy	NN
cord-347472-n6811ens	60	24	of	of	IN
cord-347472-n6811ens	60	25	the	the	DT
cord-347472-n6811ens	60	26	genome	genome	NN
cord-347472-n6811ens	60	27	copy	copy	NN
cord-347472-n6811ens	60	28	per	per	IN
cord-347472-n6811ens	60	29	reaction	reaction	NN
cord-347472-n6811ens	60	30	(	(	-LRB-
cord-347472-n6811ens	60	31	7	7	CD
cord-347472-n6811ens	60	32	)	)	-RRB-
cord-347472-n6811ens	60	33	.	.	.
cord-347472-n6811ens	61	1	Due	due	IN
cord-347472-n6811ens	61	2	to	to	IN
cord-347472-n6811ens	61	3	the	the	DT
cord-347472-n6811ens	61	4	presence	presence	NN
cord-347472-n6811ens	61	5	of	of	IN
cord-347472-n6811ens	61	6	co	co	JJ
cord-347472-n6811ens	61	7	-	-	JJ
cord-347472-n6811ens	61	8	purifying	purifying	JJ
cord-347472-n6811ens	61	9	genomic	genomic	JJ
cord-347472-n6811ens	61	10	DNA	dna	NN
cord-347472-n6811ens	61	11	in	in	IN
cord-347472-n6811ens	61	12	clinical	clinical	JJ
cord-347472-n6811ens	61	13	samples	sample	NNS
cord-347472-n6811ens	61	14	,	,	,
cord-347472-n6811ens	61	15	loss	loss	NN
cord-347472-n6811ens	61	16	of	of	IN
cord-347472-n6811ens	61	17	RNA	RNA	NNP
cord-347472-n6811ens	61	18	integrity	integrity	NN
cord-347472-n6811ens	61	19	leads	lead	VBZ
cord-347472-n6811ens	61	20	to	to	IN
cord-347472-n6811ens	61	21	false	false	JJ
cord-347472-n6811ens	61	22	-	-	HYPH
cord-347472-n6811ens	61	23	negative	negative	JJ
cord-347472-n6811ens	61	24	results	result	NNS
cord-347472-n6811ens	61	25	using	use	VBG
cord-347472-n6811ens	61	26	the	the	DT
cord-347472-n6811ens	61	27	CDC	CDC	NNP
cord-347472-n6811ens	61	28	-	-	HYPH
cord-347472-n6811ens	61	29	specified	specify	VBN
cord-347472-n6811ens	61	30	control	control	NN
cord-347472-n6811ens	61	31	.	.	.
cord-347472-n6811ens	62	1	RNA	RNA	NNP
cord-347472-n6811ens	62	2	,	,	,
cord-347472-n6811ens	62	3	including	include	VBG
cord-347472-n6811ens	62	4	the	the	DT
cord-347472-n6811ens	62	5	genome	genome	NN
cord-347472-n6811ens	62	6	of	of	IN
cord-347472-n6811ens	62	7	the	the	DT
cord-347472-n6811ens	62	8	SARS	SARS	NNP
cord-347472-n6811ens	62	9	-	-	HYPH
cord-347472-n6811ens	62	10	CoV-2	CoV-2	NNP
cord-347472-n6811ens	62	11	virus	virus	NN
cord-347472-n6811ens	62	12	,	,	,
cord-347472-n6811ens	62	13	is	be	VBZ
cord-347472-n6811ens	62	14	intrinsically	intrinsically	RB
cord-347472-n6811ens	62	15	more	more	RBR
cord-347472-n6811ens	62	16	labile	labile	JJ
cord-347472-n6811ens	62	17	than	than	IN
cord-347472-n6811ens	62	18	DNA	DNA	NNP
cord-347472-n6811ens	62	19	.	.	.
cord-347472-n6811ens	63	1	RNA	rna	NN
cord-347472-n6811ens	63	2	-	-	HYPH
cord-347472-n6811ens	63	3	degrading	degrade	VBG
cord-347472-n6811ens	63	4	enzymes	enzyme	NNS
cord-347472-n6811ens	63	5	(	(	-LRB-
cord-347472-n6811ens	63	6	RNases	RNases	NNP
cord-347472-n6811ens	63	7	)	)	-RRB-
cord-347472-n6811ens	63	8	rapidly	rapidly	RB
cord-347472-n6811ens	63	9	hydrolyze	hydrolyze	VBP
cord-347472-n6811ens	63	10	RNA	RNA	NNP
cord-347472-n6811ens	63	11	while	while	IN
cord-347472-n6811ens	63	12	leaving	leave	VBG
cord-347472-n6811ens	63	13	DNA	dna	NN
cord-347472-n6811ens	63	14	intact	intact	JJ
cord-347472-n6811ens	63	15	.	.	.
cord-347472-n6811ens	64	1	Laboratory	laboratory	NN
cord-347472-n6811ens	64	2	procedures	procedure	NNS
cord-347472-n6811ens	64	3	that	that	WDT
cord-347472-n6811ens	64	4	manipulate	manipulate	VBP
cord-347472-n6811ens	64	5	RNA	RNA	NNP
cord-347472-n6811ens	64	6	,	,	,
cord-347472-n6811ens	64	7	including	include	VBG
cord-347472-n6811ens	64	8	methods	method	NNS
cord-347472-n6811ens	64	9	specified	specify	VBN
cord-347472-n6811ens	64	10	in	in	IN
cord-347472-n6811ens	64	11	the	the	DT
cord-347472-n6811ens	64	12	CDC	CDC	NNP
cord-347472-n6811ens	64	13	EUA	EUA	NNP
cord-347472-n6811ens	64	14	,	,	,
cord-347472-n6811ens	64	15	require	require	VBP
cord-347472-n6811ens	64	16	careful	careful	JJ
cord-347472-n6811ens	64	17	sample	sample	NN
cord-347472-n6811ens	64	18	,	,	,
cord-347472-n6811ens	64	19	reagent	reagent	NN
cord-347472-n6811ens	64	20	,	,	,
cord-347472-n6811ens	64	21	and	and	CC
cord-347472-n6811ens	64	22	consumable	consumable	JJ
cord-347472-n6811ens	64	23	handling	handling	NN
cord-347472-n6811ens	64	24	to	to	TO
cord-347472-n6811ens	64	25	avoid	avoid	VB
cord-347472-n6811ens	64	26	introduction	introduction	NN
cord-347472-n6811ens	64	27	of	of	IN
cord-347472-n6811ens	64	28	ubiquitous	ubiquitous	JJ
cord-347472-n6811ens	64	29	environmental	environmental	JJ
cord-347472-n6811ens	64	30	or	or	CC
cord-347472-n6811ens	64	31	personnel	personnel	NNS
cord-347472-n6811ens	64	32	-	-	HYPH
cord-347472-n6811ens	64	33	derived	derive	VBN
cord-347472-n6811ens	64	34	RNases	rnase	NNS
cord-347472-n6811ens	64	35	.	.	.
cord-347472-n6811ens	65	1	Loss	loss	NN
cord-347472-n6811ens	65	2	of	of	IN
cord-347472-n6811ens	65	3	RNA	RNA	NNP
cord-347472-n6811ens	65	4	integrity	integrity	NN
cord-347472-n6811ens	65	5	,	,	,
cord-347472-n6811ens	65	6	accomplished	accomplish	VBN
cord-347472-n6811ens	65	7	here	here	RB
cord-347472-n6811ens	65	8	by	by	IN
cord-347472-n6811ens	65	9	intentional	intentional	JJ
cord-347472-n6811ens	65	10	exposure	exposure	NN
cord-347472-n6811ens	65	11	to	to	IN
cord-347472-n6811ens	65	12	RNase	rnase	NN
cord-347472-n6811ens	65	13	,	,	,
cord-347472-n6811ens	65	14	causes	cause	VBZ
cord-347472-n6811ens	65	15	virus	virus	NN
cord-347472-n6811ens	65	16	-	-	HYPH
cord-347472-n6811ens	65	17	positive	positive	JJ
cord-347472-n6811ens	65	18	clinical	clinical	JJ
cord-347472-n6811ens	65	19	samples	sample	NNS
cord-347472-n6811ens	65	20	to	to	TO
cord-347472-n6811ens	65	21	return	return	VB
cord-347472-n6811ens	65	22	a	a	DT
cord-347472-n6811ens	65	23	false	false	JJ
cord-347472-n6811ens	65	24	-	-	HYPH
cord-347472-n6811ens	65	25	negative	negative	JJ
cord-347472-n6811ens	65	26	"	"	``
cord-347472-n6811ens	65	27	extraction	extraction	NN
cord-347472-n6811ens	65	28	control	control	NN
cord-347472-n6811ens	65	29	positive	positive	JJ
cord-347472-n6811ens	65	30	,	,	,
cord-347472-n6811ens	65	31	virus	virus	NN
cord-347472-n6811ens	65	32	negative	negative	JJ
cord-347472-n6811ens	65	33	"	"	''
cord-347472-n6811ens	65	34	result	result	NN
cord-347472-n6811ens	65	35	using	use	VBG
cord-347472-n6811ens	65	36	the	the	DT
cord-347472-n6811ens	65	37	CDC	CDC	NNP
cord-347472-n6811ens	65	38	-	-	HYPH
cord-347472-n6811ens	65	39	design	design	NN
cord-347472-n6811ens	65	40	(	(	-LRB-
cord-347472-n6811ens	65	41	Fig	Fig	NNP
cord-347472-n6811ens	65	42	.	.	.
cord-347472-n6811ens	65	43	3	3	CD
cord-347472-n6811ens	65	44	)	)	-RRB-
cord-347472-n6811ens	65	45	.	.	.
cord-347472-n6811ens	66	1	Assays	assay	NNS
cord-347472-n6811ens	66	2	can	can	MD
cord-347472-n6811ens	66	3	be	be	VB
cord-347472-n6811ens	66	4	designed	design	VBN
cord-347472-n6811ens	66	5	to	to	TO
cord-347472-n6811ens	66	6	specifically	specifically	RB
cord-347472-n6811ens	66	7	detect	detect	VB
cord-347472-n6811ens	66	8	mature	mature	JJ
cord-347472-n6811ens	66	9	mRNA	mrna	NN
cord-347472-n6811ens	66	10	,	,	,
cord-347472-n6811ens	66	11	and	and	CC
cord-347472-n6811ens	66	12	ignore	ignore	VB
cord-347472-n6811ens	66	13	genomic	genomic	JJ
cord-347472-n6811ens	66	14	DNA	dna	NN
cord-347472-n6811ens	66	15	,	,	,
cord-347472-n6811ens	66	16	by	by	IN
cord-347472-n6811ens	66	17	generating	generate	VBG
cord-347472-n6811ens	66	18	amplicons	amplicon	NNS
cord-347472-n6811ens	66	19	that	that	WDT
cord-347472-n6811ens	66	20	are	be	VBP
cord-347472-n6811ens	66	21	of	of	IN
cord-347472-n6811ens	66	22	PCR	PCR	NNP
cord-347472-n6811ens	66	23	-	-	HYPH
cord-347472-n6811ens	66	24	amplifiable	amplifiable	JJ
cord-347472-n6811ens	66	25	size	size	NN
cord-347472-n6811ens	66	26	only	only	RB
cord-347472-n6811ens	66	27	after	after	IN
cord-347472-n6811ens	66	28	splicing	splicing	NN
cord-347472-n6811ens	66	29	or	or	CC
cord-347472-n6811ens	66	30	by	by	IN
cord-347472-n6811ens	66	31	targeting	target	VBG
cord-347472-n6811ens	66	32	sequences	sequence	NNS
cord-347472-n6811ens	66	33	created	create	VBN
cord-347472-n6811ens	66	34	at	at	IN
cord-347472-n6811ens	66	35	exon	exon	NN
cord-347472-n6811ens	66	36	-	-	HYPH
cord-347472-n6811ens	66	37	exon	exon	NN
cord-347472-n6811ens	66	38	junctions	junction	NNS
cord-347472-n6811ens	66	39	(	(	-LRB-
cord-347472-n6811ens	66	40	Figs	fig	NNS
cord-347472-n6811ens	66	41	.	.	.
cord-347472-n6811ens	67	1	3	3	LS
cord-347472-n6811ens	67	2	,	,	,
cord-347472-n6811ens	67	3	S1	S1	NNP
cord-347472-n6811ens	67	4	-	-	HYPH
cord-347472-n6811ens	67	5	3	3	CD
cord-347472-n6811ens	67	6	)	)	-RRB-
cord-347472-n6811ens	67	7	.	.	.
cord-347472-n6811ens	68	1	A	a	DT
cord-347472-n6811ens	68	2	transcript	transcript	NN
cord-347472-n6811ens	68	3	-	-	HYPH
cord-347472-n6811ens	68	4	specific	specific	JJ
cord-347472-n6811ens	68	5	control	control	NN
cord-347472-n6811ens	68	6	design	design	NN
cord-347472-n6811ens	68	7	incorporating	incorporate	VBG
cord-347472-n6811ens	68	8	both	both	DT
cord-347472-n6811ens	68	9	of	of	IN
cord-347472-n6811ens	68	10	these	these	DT
cord-347472-n6811ens	68	11	approaches	approach	NNS
cord-347472-n6811ens	68	12	correctly	correctly	RB
cord-347472-n6811ens	68	13	monitors	monitor	VBZ
cord-347472-n6811ens	68	14	human	human	JJ
cord-347472-n6811ens	68	15	specimen	specimen	NN
cord-347472-n6811ens	68	16	collection	collection	NN
cord-347472-n6811ens	68	17	,	,	,
cord-347472-n6811ens	68	18	RNA	RNA	NNP
cord-347472-n6811ens	68	19	integrity	integrity	NN
cord-347472-n6811ens	68	20	,	,	,
cord-347472-n6811ens	68	21	and	and	CC
cord-347472-n6811ens	68	22	successful	successful	JJ
cord-347472-n6811ens	68	23	reverse	reverse	NN
cord-347472-n6811ens	68	24	transcription	transcription	NN
cord-347472-n6811ens	68	25	,	,	,
cord-347472-n6811ens	68	26	thus	thus	RB
cord-347472-n6811ens	68	27	serving	serve	VBG
cord-347472-n6811ens	68	28	all	all	DT
cord-347472-n6811ens	68	29	intended	intend	VBN
cord-347472-n6811ens	68	30	roles	role	NNS
cord-347472-n6811ens	68	31	of	of	IN
cord-347472-n6811ens	68	32	the	the	DT
cord-347472-n6811ens	68	33	human	human	JJ
cord-347472-n6811ens	68	34	specimen	specimen	NN
cord-347472-n6811ens	68	35	/	/	SYM
cord-347472-n6811ens	68	36	extraction	extraction	NN
cord-347472-n6811ens	68	37	control	control	NN
cord-347472-n6811ens	68	38	(	(	-LRB-
cord-347472-n6811ens	68	39	Figs	fig	NNS
cord-347472-n6811ens	68	40	.	.	.
cord-347472-n6811ens	69	1	3	3	LS
cord-347472-n6811ens	69	2	,	,	,
cord-347472-n6811ens	69	3	S1	s1	NN
cord-347472-n6811ens	69	4	,	,	,
cord-347472-n6811ens	69	5	and	and	CC
cord-347472-n6811ens	69	6	S2	S2	NNP
cord-347472-n6811ens	69	7	and	and	CC
cord-347472-n6811ens	69	8	Supplementary	Supplementary	NNP
cord-347472-n6811ens	69	9	Text	Text	NNP
cord-347472-n6811ens	69	10	)	)	-RRB-
cord-347472-n6811ens	70	1	Discussion	discussion	NN
cord-347472-n6811ens	70	2	:	:	:
cord-347472-n6811ens	70	3	Properly	properly	RB
cord-347472-n6811ens	70	4	-	-	HYPH
cord-347472-n6811ens	70	5	designed	design	VBN
cord-347472-n6811ens	70	6	extraction	extraction	NN
cord-347472-n6811ens	70	7	and	and	CC
cord-347472-n6811ens	70	8	reverse	reverse	VB
cord-347472-n6811ens	70	9	transcription	transcription	NN
cord-347472-n6811ens	70	10	controls	control	NNS
cord-347472-n6811ens	70	11	are	be	VBP
cord-347472-n6811ens	70	12	essential	essential	JJ
cord-347472-n6811ens	70	13	,	,	,
cord-347472-n6811ens	70	14	not	not	RB
cord-347472-n6811ens	70	15	for	for	IN
cord-347472-n6811ens	70	16	detecting	detect	VBG
cord-347472-n6811ens	70	17	SARS	SARS	NNP
cord-347472-n6811ens	70	18	-	-	HYPH
cord-347472-n6811ens	70	19	CoV-2	CoV-2	NNP
cord-347472-n6811ens	70	20	,	,	,
cord-347472-n6811ens	70	21	but	but	CC
cord-347472-n6811ens	70	22	for	for	IN
cord-347472-n6811ens	70	23	determining	determine	VBG
cord-347472-n6811ens	70	24	when	when	WRB
cord-347472-n6811ens	70	25	a	a	DT
cord-347472-n6811ens	70	26	negative	negative	JJ
cord-347472-n6811ens	70	27	test	test	NN
cord-347472-n6811ens	70	28	result	result	NN
cord-347472-n6811ens	70	29	is	be	VBZ
cord-347472-n6811ens	70	30	invalid	invalid	JJ
cord-347472-n6811ens	70	31	.	.	.
cord-347472-n6811ens	71	1	The	the	DT
cord-347472-n6811ens	71	2	unprecedented	unprecedented	JJ
cord-347472-n6811ens	71	3	scale	scale	NN
cord-347472-n6811ens	71	4	of	of	IN
cord-347472-n6811ens	71	5	molecular	molecular	JJ
cord-347472-n6811ens	71	6	testing	testing	NN
cord-347472-n6811ens	71	7	undertaken	undertake	VBN
cord-347472-n6811ens	71	8	during	during	IN
cord-347472-n6811ens	71	9	the	the	DT
cord-347472-n6811ens	71	10	COVID-19	COVID-19	NNP
cord-347472-n6811ens	71	11	pandemic	pandemic	NN
cord-347472-n6811ens	71	12	has	have	VBZ
cord-347472-n6811ens	71	13	required	require	VBN
cord-347472-n6811ens	71	14	that	that	IN
cord-347472-n6811ens	71	15	labs	lab	NNS
cord-347472-n6811ens	71	16	rapidly	rapidly	RB
cord-347472-n6811ens	71	17	adopt	adopt	VBP
cord-347472-n6811ens	71	18	this	this	DT
cord-347472-n6811ens	71	19	CDC	CDC	NNP
cord-347472-n6811ens	71	20	-	-	HYPH
cord-347472-n6811ens	71	21	specified	specify	VBN
cord-347472-n6811ens	71	22	,	,	,
cord-347472-n6811ens	71	23	among	among	IN
cord-347472-n6811ens	71	24	many	many	JJ
cord-347472-n6811ens	71	25	other	other	JJ
cord-347472-n6811ens	71	26	,	,	,
cord-347472-n6811ens	71	27	new	new	JJ
cord-347472-n6811ens	71	28	assays	assay	NNS
cord-347472-n6811ens	71	29	.	.	.
cord-347472-n6811ens	72	1	Labs	lab	NNS
cord-347472-n6811ens	72	2	accustomed	accustom	VBD
cord-347472-n6811ens	72	3	to	to	IN
cord-347472-n6811ens	72	4	automated	automated	JJ
cord-347472-n6811ens	72	5	walk	walk	NN
cord-347472-n6811ens	72	6	-	-	HYPH
cord-347472-n6811ens	72	7	up	up	RP
cord-347472-n6811ens	72	8	systems	system	NNS
cord-347472-n6811ens	72	9	are	be	VBP
cord-347472-n6811ens	72	10	now	now	RB
cord-347472-n6811ens	72	11	performing	perform	VBG
cord-347472-n6811ens	72	12	manual	manual	JJ
cord-347472-n6811ens	72	13	sample	sample	NN
cord-347472-n6811ens	72	14	handling	handling	NN
cord-347472-n6811ens	72	15	and	and	CC
cord-347472-n6811ens	72	16	reaction	reaction	NN
cord-347472-n6811ens	72	17	setup	setup	NN
cord-347472-n6811ens	72	18	one	one	CD
cord-347472-n6811ens	72	19	pipet	pipet	NN
cord-347472-n6811ens	72	20	tip	tip	NN
cord-347472-n6811ens	72	21	at	at	IN
cord-347472-n6811ens	72	22	a	a	DT
cord-347472-n6811ens	72	23	time	time	NN
cord-347472-n6811ens	72	24	.	.	.
cord-347472-n6811ens	73	1	The	the	DT
cord-347472-n6811ens	73	2	CDC	CDC	NNP
cord-347472-n6811ens	73	3	design	design	NN
cord-347472-n6811ens	73	4	detects	detect	VBZ
cord-347472-n6811ens	73	5	SARS	SARS	NNP
cord-347472-n6811ens	73	6	-	-	HYPH
cord-347472-n6811ens	73	7	CoV-2	CoV-2	NNP
cord-347472-n6811ens	73	8	,	,	,
cord-347472-n6811ens	73	9	but	but	CC
cord-347472-n6811ens	73	10	in	in	IN
cord-347472-n6811ens	73	11	the	the	DT
cord-347472-n6811ens	73	12	face	face	NN
cord-347472-n6811ens	73	13	of	of	IN
cord-347472-n6811ens	73	14	real	real	JJ
cord-347472-n6811ens	73	15	-	-	HYPH
cord-347472-n6811ens	73	16	world	world	NN
cord-347472-n6811ens	73	17	assay	assay	NN
cord-347472-n6811ens	73	18	errors	error	NNS
cord-347472-n6811ens	73	19	,	,	,
cord-347472-n6811ens	73	20	its	-PRON-	PRP$
cord-347472-n6811ens	73	21	faulty	faulty	JJ
cord-347472-n6811ens	73	22	control	control	NN
cord-347472-n6811ens	73	23	design	design	NN
cord-347472-n6811ens	73	24	fails	fail	VBZ
cord-347472-n6811ens	73	25	-	-	HYPH
cord-347472-n6811ens	73	26	unsafe	unsafe	JJ
cord-347472-n6811ens	73	27	and	and	CC
cord-347472-n6811ens	73	28	calls	call	VBZ
cord-347472-n6811ens	73	29	into	into	IN
cord-347472-n6811ens	73	30	question	question	NN
cord-347472-n6811ens	73	31	virus	virus	NN
cord-347472-n6811ens	73	32	-	-	HYPH
cord-347472-n6811ens	73	33	negative	negative	JJ
cord-347472-n6811ens	73	34	results	result	NNS
cord-347472-n6811ens	73	35	.	.	.
cord-347472-n6811ens	74	1	The	the	DT
cord-347472-n6811ens	74	2	CDC	CDC	NNP
cord-347472-n6811ens	74	3	specifies	specify	VBZ
cord-347472-n6811ens	74	4	a	a	DT
cord-347472-n6811ens	74	5	once	once	RB
cord-347472-n6811ens	74	6	-	-	HYPH
cord-347472-n6811ens	74	7	per	per	IN
cord-347472-n6811ens	74	8	-	-	HYPH
cord-347472-n6811ens	74	9	plate	plate	NN
cord-347472-n6811ens	74	10	control	control	NN
cord-347472-n6811ens	74	11	using	use	VBG
cord-347472-n6811ens	74	12	in	in	IN
cord-347472-n6811ens	74	13	vitro	vitro	FW
cord-347472-n6811ens	74	14	transcribed	transcribe	VBN
cord-347472-n6811ens	74	15	RNA	RNA	NNP
cord-347472-n6811ens	74	16	,	,	,
cord-347472-n6811ens	74	17	purified	purify	VBN
cord-347472-n6811ens	74	18	virus	virus	NN
cord-347472-n6811ens	74	19	,	,	,
cord-347472-n6811ens	74	20	or	or	CC
cord-347472-n6811ens	74	21	pooled	pool	VBN
cord-347472-n6811ens	74	22	patient	patient	NN
cord-347472-n6811ens	74	23	samples	sample	NNS
cord-347472-n6811ens	74	24	.	.	.
cord-347472-n6811ens	75	1	This	this	DT
cord-347472-n6811ens	75	2	approach	approach	NN
cord-347472-n6811ens	75	3	does	do	VBZ
cord-347472-n6811ens	75	4	not	not	RB
cord-347472-n6811ens	75	5	inform	inform	VB
cord-347472-n6811ens	75	6	about	about	IN
cord-347472-n6811ens	75	7	the	the	DT
cord-347472-n6811ens	75	8	suitability	suitability	NN
cord-347472-n6811ens	75	9	of	of	IN
cord-347472-n6811ens	75	10	individual	individual	JJ
cord-347472-n6811ens	75	11	specimens	specimen	NNS
cord-347472-n6811ens	75	12	.	.	.
cord-347472-n6811ens	76	1	Degradation	degradation	NN
cord-347472-n6811ens	76	2	of	of	IN
cord-347472-n6811ens	76	3	sample	sample	NN
cord-347472-n6811ens	76	4	RNAs	rna	NNS
cord-347472-n6811ens	76	5	or	or	CC
cord-347472-n6811ens	76	6	failure	failure	NN
cord-347472-n6811ens	76	7	of	of	IN
cord-347472-n6811ens	76	8	reverse	reverse	JJ
cord-347472-n6811ens	76	9	transcription	transcription	NN
cord-347472-n6811ens	76	10	on	on	IN
cord-347472-n6811ens	76	11	a	a	DT
cord-347472-n6811ens	76	12	per	per	IN
cord-347472-n6811ens	76	13	-	-	HYPH
cord-347472-n6811ens	76	14	sample	sample	NN
cord-347472-n6811ens	76	15	basis	basis	NN
cord-347472-n6811ens	76	16	results	result	NNS
cord-347472-n6811ens	76	17	in	in	IN
cord-347472-n6811ens	76	18	the	the	DT
cord-347472-n6811ens	76	19	inability	inability	NN
cord-347472-n6811ens	76	20	to	to	TO
cord-347472-n6811ens	76	21	detect	detect	VB
cord-347472-n6811ens	76	22	viral	viral	JJ
cord-347472-n6811ens	76	23	RNAs	rna	NNS
cord-347472-n6811ens	76	24	while	while	IN
cord-347472-n6811ens	76	25	the	the	DT
cord-347472-n6811ens	76	26	DNA	dna	NN
cord-347472-n6811ens	76	27	-	-	HYPH
cord-347472-n6811ens	76	28	templated	templated	JJ
cord-347472-n6811ens	76	29	control	control	NN
cord-347472-n6811ens	76	30	signal	signal	NN
cord-347472-n6811ens	76	31	remains	remain	VBZ
cord-347472-n6811ens	76	32	.	.	.
cord-347472-n6811ens	77	1	This	this	DT
cord-347472-n6811ens	77	2	opens	open	VBZ
cord-347472-n6811ens	77	3	the	the	DT
cord-347472-n6811ens	77	4	door	door	NN
cord-347472-n6811ens	77	5	to	to	IN
cord-347472-n6811ens	77	6	false	false	JJ
cord-347472-n6811ens	77	7	-	-	HYPH
cord-347472-n6811ens	77	8	negative	negative	JJ
cord-347472-n6811ens	77	9	clinical	clinical	JJ
cord-347472-n6811ens	77	10	interpretation	interpretation	NN
cord-347472-n6811ens	77	11	of	of	IN
cord-347472-n6811ens	77	12	patients	patient	NNS
cord-347472-n6811ens	77	13	from	from	IN
cord-347472-n6811ens	77	14	whom	whom	WP
cord-347472-n6811ens	77	15	virus	virus	NN
cord-347472-n6811ens	77	16	was	be	VBD
cord-347472-n6811ens	77	17	successfully	successfully	RB
cord-347472-n6811ens	77	18	collected	collect	VBN
cord-347472-n6811ens	77	19	.	.	.
cord-347472-n6811ens	78	1	Controls	control	NNS
cord-347472-n6811ens	78	2	for	for	IN
cord-347472-n6811ens	78	3	specimen	specimen	JJ
cord-347472-n6811ens	78	4	integrity	integrity	NN
cord-347472-n6811ens	78	5	and	and	CC
cord-347472-n6811ens	78	6	success	success	NN
cord-347472-n6811ens	78	7	of	of	IN
cord-347472-n6811ens	78	8	all	all	DT
cord-347472-n6811ens	78	9	reaction	reaction	NN
cord-347472-n6811ens	78	10	steps	step	NNS
cord-347472-n6811ens	78	11	are	be	VBP
cord-347472-n6811ens	78	12	critical	critical	JJ
cord-347472-n6811ens	78	13	to	to	IN
cord-347472-n6811ens	78	14	robust	robust	JJ
cord-347472-n6811ens	78	15	assay	assay	NN
cord-347472-n6811ens	78	16	design	design	NN
cord-347472-n6811ens	78	17	.	.	.
cord-347472-n6811ens	79	1	The	the	DT
cord-347472-n6811ens	79	2	currently	currently	RB
cord-347472-n6811ens	79	3	-	-	HYPH
cord-347472-n6811ens	79	4	specified	specify	VBN
cord-347472-n6811ens	79	5	CDC	CDC	NNP
cord-347472-n6811ens	79	6	control	control	NN
cord-347472-n6811ens	79	7	achieves	achieve	VBZ
cord-347472-n6811ens	79	8	neither	neither	RB
cord-347472-n6811ens	79	9	.	.	.
cord-347472-n6811ens	80	1	A	a	DT
cord-347472-n6811ens	80	2	number	number	NN
cord-347472-n6811ens	80	3	of	of	IN
cord-347472-n6811ens	80	4	other	other	JJ
cord-347472-n6811ens	80	5	FDAapproved	FDAapproved	NNP
cord-347472-n6811ens	80	6	SARS	SARS	NNP
cord-347472-n6811ens	80	7	-	-	HYPH
cord-347472-n6811ens	80	8	CoV-2	CoV-2	NNP
cord-347472-n6811ens	80	9	molecular	molecular	JJ
cord-347472-n6811ens	80	10	assays	assay	NNS
cord-347472-n6811ens	80	11	use	use	VBP
cord-347472-n6811ens	80	12	synthetic	synthetic	JJ
cord-347472-n6811ens	80	13	RNA	RNA	NNP
cord-347472-n6811ens	80	14	spike	spike	NN
cord-347472-n6811ens	80	15	-	-	HYPH
cord-347472-n6811ens	80	16	ins	in	NNS
cord-347472-n6811ens	80	17	added	add	VBD
cord-347472-n6811ens	80	18	at	at	IN
cord-347472-n6811ens	80	19	the	the	DT
cord-347472-n6811ens	80	20	time	time	NN
cord-347472-n6811ens	80	21	of	of	IN
cord-347472-n6811ens	80	22	extraction	extraction	NN
cord-347472-n6811ens	80	23	.	.	.
cord-347472-n6811ens	81	1	This	this	DT
cord-347472-n6811ens	81	2	approach	approach	NN
cord-347472-n6811ens	81	3	controls	control	VBZ
cord-347472-n6811ens	81	4	for	for	IN
cord-347472-n6811ens	81	5	post	post	JJ
cord-347472-n6811ens	81	6	-	-	JJ
cord-347472-n6811ens	81	7	extraction	extraction	JJ
cord-347472-n6811ens	81	8	sample	sample	NN
cord-347472-n6811ens	81	9	integrity	integrity	NN
cord-347472-n6811ens	81	10	and	and	CC
cord-347472-n6811ens	81	11	reverse	reverse	VB
cord-347472-n6811ens	81	12	transcription	transcription	NN
cord-347472-n6811ens	81	13	,	,	,
cord-347472-n6811ens	81	14	but	but	CC
cord-347472-n6811ens	81	15	does	do	VBZ
cord-347472-n6811ens	81	16	not	not	RB
cord-347472-n6811ens	81	17	inform	inform	VB
cord-347472-n6811ens	81	18	about	about	IN
cord-347472-n6811ens	81	19	sufficiency	sufficiency	NN
cord-347472-n6811ens	81	20	of	of	IN
cord-347472-n6811ens	81	21	collected	collect	VBN
cord-347472-n6811ens	81	22	patient	patient	NN
cord-347472-n6811ens	81	23	samples	sample	NNS
cord-347472-n6811ens	81	24	(	(	-LRB-
cord-347472-n6811ens	81	25	9	9	CD
cord-347472-n6811ens	81	26	)	)	-RRB-
cord-347472-n6811ens	81	27	.	.	.
cord-347472-n6811ens	82	1	The	the	DT
cord-347472-n6811ens	82	2	intent	intent	NN
cord-347472-n6811ens	82	3	of	of	IN
cord-347472-n6811ens	82	4	the	the	DT
cord-347472-n6811ens	82	5	CDC	CDC	NNP
cord-347472-n6811ens	82	6	's	's	POS
cord-347472-n6811ens	82	7	extraction	extraction	NN
cord-347472-n6811ens	82	8	control	control	NN
cord-347472-n6811ens	82	9	is	be	VBZ
cord-347472-n6811ens	82	10	good	good	JJ
cord-347472-n6811ens	82	11	,	,	,
cord-347472-n6811ens	82	12	monitoring	monitoring	NN
cord-347472-n6811ens	82	13	(	(	-LRB-
cord-347472-n6811ens	82	14	1	1	CD
cord-347472-n6811ens	82	15	)	)	-RRB-
cord-347472-n6811ens	82	16	sufficient	sufficient	JJ
cord-347472-n6811ens	82	17	human	human	JJ
cord-347472-n6811ens	82	18	specimen	specimen	NN
cord-347472-n6811ens	82	19	collection	collection	NN
cord-347472-n6811ens	82	20	,	,	,
cord-347472-n6811ens	82	21	(	(	-LRB-
cord-347472-n6811ens	82	22	2	2	LS
cord-347472-n6811ens	82	23	)	)	-RRB-
cord-347472-n6811ens	82	24	maintenance	maintenance	NN
cord-347472-n6811ens	82	25	of	of	IN
cord-347472-n6811ens	82	26	sample	sample	NN
cord-347472-n6811ens	82	27	integrity	integrity	NN
cord-347472-n6811ens	82	28	during	during	IN
cord-347472-n6811ens	82	29	processing	processing	NN
cord-347472-n6811ens	82	30	,	,	,
cord-347472-n6811ens	82	31	and	and	CC
cord-347472-n6811ens	82	32	(	(	-LRB-
cord-347472-n6811ens	82	33	3	3	LS
cord-347472-n6811ens	82	34	)	)	-RRB-
cord-347472-n6811ens	82	35	successful	successful	JJ
cord-347472-n6811ens	82	36	reverse	reverse	NN
cord-347472-n6811ens	82	37	transcription	transcription	NN
cord-347472-n6811ens	82	38	and	and	CC
cord-347472-n6811ens	82	39	qPCR	qpcr	NN
cord-347472-n6811ens	82	40	.	.	.
cord-347472-n6811ens	83	1	These	these	DT
cord-347472-n6811ens	83	2	goals	goal	NNS
cord-347472-n6811ens	83	3	can	can	MD
cord-347472-n6811ens	83	4	be	be	VB
cord-347472-n6811ens	83	5	met	meet	VBN
cord-347472-n6811ens	83	6	by	by	IN
cord-347472-n6811ens	83	7	an	an	DT
cord-347472-n6811ens	83	8	extraction	extraction	NN
cord-347472-n6811ens	83	9	control	control	NN
cord-347472-n6811ens	83	10	that	that	WDT
cord-347472-n6811ens	83	11	specifically	specifically	RB
cord-347472-n6811ens	83	12	detects	detect	VBZ
cord-347472-n6811ens	83	13	human	human	JJ
cord-347472-n6811ens	83	14	RNA	RNA	NNP
cord-347472-n6811ens	83	15	and	and	CC
cord-347472-n6811ens	83	16	is	be	VBZ
cord-347472-n6811ens	83	17	insensitive	insensitive	JJ
cord-347472-n6811ens	83	18	to	to	TO
cord-347472-n6811ens	83	19	genomic	genomic	JJ
cord-347472-n6811ens	83	20	DNA	dna	NN
cord-347472-n6811ens	83	21	;	;	:
cord-347472-n6811ens	83	22	the	the	DT
cord-347472-n6811ens	83	23	CDC	CDC	NNP
cord-347472-n6811ens	83	24	's	's	POS
cord-347472-n6811ens	83	25	mis	mis	NN
cord-347472-n6811ens	83	26	-	-	HYPH
cord-347472-n6811ens	83	27	executed	execute	VBN
cord-347472-n6811ens	83	28	plan	plan	NN
cord-347472-n6811ens	83	29	can	can	MD
cord-347472-n6811ens	83	30	be	be	VB
cord-347472-n6811ens	83	31	put	put	VBN
cord-347472-n6811ens	83	32	into	into	IN
cord-347472-n6811ens	83	33	practice	practice	NN
cord-347472-n6811ens	83	34	by	by	IN
cord-347472-n6811ens	83	35	manufacturing	manufacture	VBG
cord-347472-n6811ens	83	36	and	and	CC
cord-347472-n6811ens	83	37	shipping	ship	VBG
cord-347472-n6811ens	83	38	an	an	DT
cord-347472-n6811ens	83	39	updated	update	VBN
cord-347472-n6811ens	83	40	control	control	NN
cord-347472-n6811ens	83	41	.	.	.
cord-347472-n6811ens	84	1	The	the	DT
cord-347472-n6811ens	84	2	DNA	dna	NN
cord-347472-n6811ens	84	3	cross	cross	JJ
cord-347472-n6811ens	84	4	-	-	JJ
cord-347472-n6811ens	84	5	reactive	reactive	JJ
cord-347472-n6811ens	84	6	control	control	NN
cord-347472-n6811ens	84	7	probe	probe	NN
cord-347472-n6811ens	84	8	is	be	VBZ
cord-347472-n6811ens	84	9	used	use	VBN
cord-347472-n6811ens	84	10	in	in	IN
cord-347472-n6811ens	84	11	over	over	IN
cord-347472-n6811ens	84	12	40	40	CD
cord-347472-n6811ens	84	13	%	%	NN
cord-347472-n6811ens	84	14	of	of	IN
cord-347472-n6811ens	84	15	the	the	DT
cord-347472-n6811ens	84	16	COVID-19	COVID-19	NNP
cord-347472-n6811ens	84	17	FDA	FDA	NNP
cord-347472-n6811ens	84	18	-	-	HYPH
cord-347472-n6811ens	84	19	authorized	authorize	VBN
cord-347472-n6811ens	84	20	protocols	protocol	NNS
cord-347472-n6811ens	84	21	listed	list	VBN
cord-347472-n6811ens	84	22	as	as	IN
cord-347472-n6811ens	84	23	of	of	IN
cord-347472-n6811ens	84	24	May	May	NNP
cord-347472-n6811ens	84	25	13	13	CD
cord-347472-n6811ens	84	26	,	,	,
cord-347472-n6811ens	84	27	2020	2020	CD
cord-347472-n6811ens	84	28	(	(	-LRB-
cord-347472-n6811ens	84	29	10	10	CD
cord-347472-n6811ens	84	30	)	)	-RRB-
cord-347472-n6811ens	84	31	.	.	.
cord-347472-n6811ens	85	1	31	31	CD
cord-347472-n6811ens	85	2	million	million	CD
cord-347472-n6811ens	85	3	assays	assay	NNS
cord-347472-n6811ens	85	4	worth	worth	JJ
cord-347472-n6811ens	85	5	of	of	IN
cord-347472-n6811ens	85	6	CDC	CDC	NNP
cord-347472-n6811ens	85	7	-	-	HYPH
cord-347472-n6811ens	85	8	design	design	NN
cord-347472-n6811ens	85	9	reagents	reagent	NNS
cord-347472-n6811ens	85	10	have	have	VBP
cord-347472-n6811ens	85	11	been	be	VBN
cord-347472-n6811ens	85	12	produced	produce	VBN
cord-347472-n6811ens	85	13	and	and	CC
cord-347472-n6811ens	85	14	shipped	ship	VBN
cord-347472-n6811ens	85	15	by	by	IN
cord-347472-n6811ens	85	16	a	a	DT
cord-347472-n6811ens	85	17	single	single	JJ
cord-347472-n6811ens	85	18	vendor	vendor	NN
cord-347472-n6811ens	85	19	as	as	IN
cord-347472-n6811ens	85	20	of	of	IN
cord-347472-n6811ens	85	21	April	April	NNP
cord-347472-n6811ens	85	22	22	22	CD
cord-347472-n6811ens	85	23	,	,	,
cord-347472-n6811ens	85	24	2020	2020	CD
cord-347472-n6811ens	85	25	(	(	-LRB-
cord-347472-n6811ens	85	26	11	11	CD
cord-347472-n6811ens	85	27	)	)	-RRB-
cord-347472-n6811ens	85	28	.	.	.
cord-347472-n6811ens	86	1	This	this	DT
cord-347472-n6811ens	86	2	CDC	CDC	NNP
cord-347472-n6811ens	86	3	-	-	HYPH
cord-347472-n6811ens	86	4	designed	design	VBN
cord-347472-n6811ens	86	5	SARS	SARS	NNP
cord-347472-n6811ens	86	6	-	-	HYPH
cord-347472-n6811ens	86	7	CoV-2	CoV-2	NNP
cord-347472-n6811ens	86	8	assay	assay	NN
cord-347472-n6811ens	86	9	is	be	VBZ
cord-347472-n6811ens	86	10	FDA	FDA	NNP
cord-347472-n6811ens	86	11	-	-	HYPH
cord-347472-n6811ens	86	12	approved	approve	VBN
cord-347472-n6811ens	86	13	and	and	CC
cord-347472-n6811ens	86	14	unquestionably	unquestionably	RB
cord-347472-n6811ens	86	15	popular	popular	JJ
cord-347472-n6811ens	86	16	,	,	,
cord-347472-n6811ens	86	17	but	but	CC
cord-347472-n6811ens	86	18	we	-PRON-	PRP
cord-347472-n6811ens	86	19	must	must	MD
cord-347472-n6811ens	86	20	not	not	RB
cord-347472-n6811ens	86	21	confuse	confuse	VB
cord-347472-n6811ens	86	22	popularity	popularity	NN
cord-347472-n6811ens	86	23	and	and	CC
cord-347472-n6811ens	86	24	official	official	JJ
cord-347472-n6811ens	86	25	approval	approval	NN
cord-347472-n6811ens	86	26	with	with	IN
cord-347472-n6811ens	86	27	being	be	VBG
cord-347472-n6811ens	86	28	correct	correct	JJ
cord-347472-n6811ens	86	29	.	.	.
cord-347472-n6811ens	87	1	The	the	DT
cord-347472-n6811ens	87	2	scope	scope	NN
cord-347472-n6811ens	87	3	of	of	IN
cord-347472-n6811ens	87	4	this	this	DT
cord-347472-n6811ens	87	5	problem	problem	NN
cord-347472-n6811ens	87	6	is	be	VBZ
cord-347472-n6811ens	87	7	large	large	JJ
cord-347472-n6811ens	87	8	,	,	,
cord-347472-n6811ens	87	9	though	though	IN
cord-347472-n6811ens	87	10	its	-PRON-	PRP$
cord-347472-n6811ens	87	11	extent	extent	NN
cord-347472-n6811ens	87	12	is	be	VBZ
cord-347472-n6811ens	87	13	unknowable	unknowable	JJ
cord-347472-n6811ens	87	14	:	:	:
cord-347472-n6811ens	87	15	the	the	DT
cord-347472-n6811ens	87	16	sequences	sequence	NNS
cord-347472-n6811ens	87	17	used	use	VBN
cord-347472-n6811ens	87	18	in	in	IN
cord-347472-n6811ens	87	19	many	many	JJ
cord-347472-n6811ens	87	20	test	test	NN
cord-347472-n6811ens	87	21	designs	design	NNS
cord-347472-n6811ens	87	22	,	,	,
cord-347472-n6811ens	87	23	commercial	commercial	JJ
cord-347472-n6811ens	87	24	and	and	CC
cord-347472-n6811ens	87	25	academic	academic	JJ
cord-347472-n6811ens	87	26	,	,	,
cord-347472-n6811ens	87	27	are	be	VBP
cord-347472-n6811ens	87	28	not	not	RB
cord-347472-n6811ens	87	29	disclosed	disclose	VBN
cord-347472-n6811ens	87	30	to	to	IN
cord-347472-n6811ens	87	31	the	the	DT
cord-347472-n6811ens	87	32	public	public	NN
cord-347472-n6811ens	87	33	.	.	.
cord-347472-n6811ens	88	1	Even	even	RB
cord-347472-n6811ens	88	2	fully	fully	RB
cord-347472-n6811ens	88	3	disclosed	disclose	VBN
cord-347472-n6811ens	88	4	sequences	sequence	NNS
cord-347472-n6811ens	88	5	do	do	VBP
cord-347472-n6811ens	88	6	not	not	RB
cord-347472-n6811ens	88	7	guarantee	guarantee	VB
cord-347472-n6811ens	88	8	rapid	rapid	JJ
cord-347472-n6811ens	88	9	discovery	discovery	NN
cord-347472-n6811ens	88	10	of	of	IN
cord-347472-n6811ens	88	11	errors	error	NNS
cord-347472-n6811ens	88	12	.	.	.
cord-347472-n6811ens	89	1	This	this	DT
cord-347472-n6811ens	89	2	poorly	poorly	RB
cord-347472-n6811ens	89	3	-	-	HYPH
cord-347472-n6811ens	89	4	designed	design	VBN
cord-347472-n6811ens	89	5	CDC	CDC	NNP
cord-347472-n6811ens	89	6	extraction	extraction	NN
cord-347472-n6811ens	89	7	control	control	NN
cord-347472-n6811ens	89	8	probe	probe	NN
cord-347472-n6811ens	89	9	has	have	VBZ
cord-347472-n6811ens	89	10	been	be	VBN
cord-347472-n6811ens	89	11	included	include	VBN
cord-347472-n6811ens	89	12	in	in	IN
cord-347472-n6811ens	89	13	clinical	clinical	JJ
cord-347472-n6811ens	89	14	RT	rt	NN
cord-347472-n6811ens	89	15	-	-	HYPH
cord-347472-n6811ens	89	16	qPCR	qpcr	NN
cord-347472-n6811ens	89	17	panels	panel	NNS
cord-347472-n6811ens	89	18	used	use	VBN
cord-347472-n6811ens	89	19	in	in	IN
cord-347472-n6811ens	89	20	testing	testing	NN
cord-347472-n6811ens	89	21	for	for	IN
cord-347472-n6811ens	89	22	RNA	RNA	NNP
cord-347472-n6811ens	89	23	viruses	virus	NNS
cord-347472-n6811ens	89	24	across	across	IN
cord-347472-n6811ens	89	25	more	more	JJR
cord-347472-n6811ens	89	26	than	than	IN
cord-347472-n6811ens	89	27	a	a	DT
cord-347472-n6811ens	89	28	decade	decade	NN
cord-347472-n6811ens	89	29	(	(	-LRB-
cord-347472-n6811ens	89	30	12	12	CD
cord-347472-n6811ens	89	31	)	)	-RRB-
cord-347472-n6811ens	89	32	.	.	.
cord-347472-n6811ens	90	1	Past	past	NN
cord-347472-n6811ens	90	2	errors	error	NNS
cord-347472-n6811ens	90	3	can	can	MD
cord-347472-n6811ens	90	4	not	not	RB
cord-347472-n6811ens	90	5	be	be	VB
cord-347472-n6811ens	90	6	unmade	unmade	JJ
cord-347472-n6811ens	90	7	,	,	,
cord-347472-n6811ens	90	8	but	but	CC
cord-347472-n6811ens	90	9	future	future	JJ
cord-347472-n6811ens	90	10	false	false	JJ
cord-347472-n6811ens	90	11	negatives	negative	NNS
cord-347472-n6811ens	90	12	that	that	WDT
cord-347472-n6811ens	90	13	would	would	MD
cord-347472-n6811ens	90	14	arise	arise	VB
cord-347472-n6811ens	90	15	from	from	IN
cord-347472-n6811ens	90	16	this	this	DT
cord-347472-n6811ens	90	17	design	design	NN
cord-347472-n6811ens	90	18	can	can	MD
cord-347472-n6811ens	90	19	be	be	VB
cord-347472-n6811ens	90	20	prevented	prevent	VBN
cord-347472-n6811ens	90	21	.	.	.
cord-347472-n6811ens	91	1	The	the	DT
cord-347472-n6811ens	91	2	current	current	JJ
cord-347472-n6811ens	91	3	CDC	CDC	NNP
cord-347472-n6811ens	91	4	algorithm	algorithm	NN
cord-347472-n6811ens	91	5	states	state	VBZ
cord-347472-n6811ens	91	6	that	that	IN
cord-347472-n6811ens	91	7	molecular	molecular	JJ
cord-347472-n6811ens	91	8	testing	testing	NN
cord-347472-n6811ens	91	9	"	"	''
cord-347472-n6811ens	91	10	…	…	NFP
cord-347472-n6811ens	91	11	must	must	MD
cord-347472-n6811ens	91	12	be	be	VB
cord-347472-n6811ens	91	13	combined	combine	VBN
cord-347472-n6811ens	91	14	with	with	IN
cord-347472-n6811ens	91	15	clinical	clinical	JJ
cord-347472-n6811ens	91	16	observations	observation	NNS
cord-347472-n6811ens	91	17	,	,	,
cord-347472-n6811ens	91	18	patient	patient	JJ
cord-347472-n6811ens	91	19	history	history	NN
cord-347472-n6811ens	91	20	,	,	,
cord-347472-n6811ens	91	21	and	and	CC
cord-347472-n6811ens	91	22	epidemiological	epidemiological	JJ
cord-347472-n6811ens	91	23	information	information	NN
cord-347472-n6811ens	91	24	"	"	''
cord-347472-n6811ens	91	25	(	(	-LRB-
cord-347472-n6811ens	91	26	2	2	CD
cord-347472-n6811ens	91	27	)	)	-RRB-
cord-347472-n6811ens	91	28	.	.	.
cord-347472-n6811ens	92	1	In	in	IN
cord-347472-n6811ens	92	2	the	the	DT
cord-347472-n6811ens	92	3	context	context	NN
cord-347472-n6811ens	92	4	of	of	IN
cord-347472-n6811ens	92	5	drive	drive	NN
cord-347472-n6811ens	92	6	-	-	HYPH
cord-347472-n6811ens	92	7	up	up	NN
cord-347472-n6811ens	92	8	testing	testing	NN
cord-347472-n6811ens	92	9	and	and	CC
cord-347472-n6811ens	92	10	potential	potential	JJ
cord-347472-n6811ens	92	11	nationwide	nationwide	JJ
cord-347472-n6811ens	92	12	return	return	NN
cord-347472-n6811ens	92	13	-	-	HYPH
cord-347472-n6811ens	92	14	to	to	IN
cord-347472-n6811ens	92	15	-	-	HYPH
cord-347472-n6811ens	92	16	work	work	NN
cord-347472-n6811ens	92	17	testing	testing	NN
cord-347472-n6811ens	92	18	,	,	,
cord-347472-n6811ens	92	19	clinical	clinical	JJ
cord-347472-n6811ens	92	20	observations	observation	NNS
cord-347472-n6811ens	92	21	will	will	MD
cord-347472-n6811ens	92	22	be	be	VB
cord-347472-n6811ens	92	23	necessarily	necessarily	RB
cord-347472-n6811ens	92	24	limited	limit	VBN
cord-347472-n6811ens	92	25	and	and	CC
cord-347472-n6811ens	92	26	often	often	RB
cord-347472-n6811ens	92	27	impersonal	impersonal	JJ
cord-347472-n6811ens	92	28	in	in	IN
cord-347472-n6811ens	92	29	scope	scope	NN
cord-347472-n6811ens	92	30	.	.	.
cord-347472-n6811ens	93	1	Absence	absence	NN
cord-347472-n6811ens	93	2	of	of	IN
cord-347472-n6811ens	93	3	data	datum	NNS
cord-347472-n6811ens	93	4	and	and	CC
cord-347472-n6811ens	93	5	data	datum	NNS
cord-347472-n6811ens	93	6	of	of	IN
cord-347472-n6811ens	93	7	absence	absence	NN
cord-347472-n6811ens	93	8	must	must	MD
cord-347472-n6811ens	93	9	be	be	VB
cord-347472-n6811ens	93	10	carefully	carefully	RB
cord-347472-n6811ens	93	11	distinguished	distinguish	VBN
cord-347472-n6811ens	93	12	.	.	.
cord-347472-n6811ens	94	1	A	a	DT
cord-347472-n6811ens	94	2	proper	proper	JJ
cord-347472-n6811ens	94	3	control	control	NN
cord-347472-n6811ens	94	4	does	do	VBZ
cord-347472-n6811ens	94	5	not	not	RB
cord-347472-n6811ens	94	6	provide	provide	VB
cord-347472-n6811ens	94	7	definitive	definitive	JJ
cord-347472-n6811ens	94	8	diagnostic	diagnostic	JJ
cord-347472-n6811ens	94	9	results	result	NNS
cord-347472-n6811ens	94	10	from	from	IN
cord-347472-n6811ens	94	11	a	a	DT
cord-347472-n6811ens	94	12	flawed	flawed	JJ
cord-347472-n6811ens	94	13	specimen	specimen	NN
cord-347472-n6811ens	94	14	.	.	.
cord-347472-n6811ens	95	1	It	-PRON-	PRP
cord-347472-n6811ens	95	2	will	will	MD
cord-347472-n6811ens	95	3	,	,	,
cord-347472-n6811ens	95	4	however	however	RB
cord-347472-n6811ens	95	5	,	,	,
cord-347472-n6811ens	95	6	fail	fail	VB
cord-347472-n6811ens	95	7	-	-	HYPH
cord-347472-n6811ens	95	8	safe	safe	JJ
cord-347472-n6811ens	95	9	,	,	,
cord-347472-n6811ens	95	10	flag	flag	VB
cord-347472-n6811ens	95	11	a	a	DT
cord-347472-n6811ens	95	12	result	result	NN
cord-347472-n6811ens	95	13	as	as	IN
cord-347472-n6811ens	95	14	invalid	invalid	JJ
cord-347472-n6811ens	95	15	,	,	,
cord-347472-n6811ens	95	16	and	and	CC
cord-347472-n6811ens	95	17	call	call	VB
cord-347472-n6811ens	95	18	attention	attention	NN
cord-347472-n6811ens	95	19	to	to	IN
cord-347472-n6811ens	95	20	problem	problem	NN
cord-347472-n6811ens	95	21	samples	sample	NNS
cord-347472-n6811ens	95	22	to	to	TO
cord-347472-n6811ens	95	23	enable	enable	VB
cord-347472-n6811ens	95	24	focused	focus	VBN
cord-347472-n6811ens	95	25	re	re	NN
cord-347472-n6811ens	95	26	-	-	NN
cord-347472-n6811ens	95	27	testing	testing	NN
cord-347472-n6811ens	95	28	or	or	CC
cord-347472-n6811ens	95	29	changes	change	NNS
cord-347472-n6811ens	95	30	in	in	IN
cord-347472-n6811ens	95	31	clinical	clinical	JJ
cord-347472-n6811ens	95	32	management	management	NN
cord-347472-n6811ens	95	33	.	.	.
cord-347472-n6811ens	96	1	In	in	IN
cord-347472-n6811ens	96	2	the	the	DT
cord-347472-n6811ens	96	3	interest	interest	NN
cord-347472-n6811ens	96	4	of	of	IN
cord-347472-n6811ens	96	5	public	public	JJ
cord-347472-n6811ens	96	6	health	health	NN
cord-347472-n6811ens	96	7	,	,	,
cord-347472-n6811ens	96	8	assays	assay	NNS
cord-347472-n6811ens	96	9	must	must	MD
cord-347472-n6811ens	96	10	generate	generate	VB
cord-347472-n6811ens	96	11	as	as	IN
cord-347472-n6811ens	96	12	many	many	JJ
cord-347472-n6811ens	96	13	valid	valid	JJ
cord-347472-n6811ens	96	14	results	result	NNS
cord-347472-n6811ens	96	15	as	as	RB
cord-347472-n6811ens	96	16	feasible	feasible	JJ
cord-347472-n6811ens	96	17	.	.	.
cord-347472-n6811ens	97	1	This	this	DT
cord-347472-n6811ens	97	2	requires	require	VBZ
cord-347472-n6811ens	97	3	simultaneously	simultaneously	RB
cord-347472-n6811ens	97	4	maximizing	maximize	VBG
cord-347472-n6811ens	97	5	identification	identification	NN
cord-347472-n6811ens	97	6	of	of	IN
cord-347472-n6811ens	97	7	true	true	JJ
cord-347472-n6811ens	97	8	positives	positive	NNS
cord-347472-n6811ens	97	9	and	and	CC
cord-347472-n6811ens	97	10	minimizing	minimize	VBG
cord-347472-n6811ens	97	11	false	false	JJ
cord-347472-n6811ens	97	12	negatives	negative	NNS
cord-347472-n6811ens	97	13	.	.	.
cord-347472-n6811ens	98	1	Virus	virus	NN
cord-347472-n6811ens	98	2	-	-	HYPH
cord-347472-n6811ens	98	3	shedding	shed	VBG
cord-347472-n6811ens	98	4	patients	patient	NNS
cord-347472-n6811ens	98	5	must	must	MD
cord-347472-n6811ens	98	6	not	not	RB
cord-347472-n6811ens	98	7	be	be	VB
cord-347472-n6811ens	98	8	given	give	VBN
cord-347472-n6811ens	98	9	an	an	DT
cord-347472-n6811ens	98	10	all	all	RB
cord-347472-n6811ens	98	11	-	-	HYPH
cord-347472-n6811ens	98	12	clear	clear	JJ
cord-347472-n6811ens	98	13	test	test	NN
cord-347472-n6811ens	98	14	result	result	NN
cord-347472-n6811ens	98	15	due	due	IN
cord-347472-n6811ens	98	16	to	to	IN
cord-347472-n6811ens	98	17	an	an	DT
cord-347472-n6811ens	98	18	improperly	improperly	RB
cord-347472-n6811ens	98	19	designed	design	VBN
cord-347472-n6811ens	98	20	that	that	WDT
cord-347472-n6811ens	98	21	can	can	MD
cord-347472-n6811ens	98	22	be	be	VB
cord-347472-n6811ens	98	23	readily	readily	RB
cord-347472-n6811ens	98	24	fixed	fix	VBN
cord-347472-n6811ens	98	25	.	.	.
cord-347472-n6811ens	99	1	While	while	IN
cord-347472-n6811ens	99	2	perfect	perfect	JJ
cord-347472-n6811ens	99	3	diagnostic	diagnostic	JJ
cord-347472-n6811ens	99	4	tests	test	NNS
cord-347472-n6811ens	99	5	are	be	VBP
cord-347472-n6811ens	99	6	unachievable	unachievable	JJ
cord-347472-n6811ens	99	7	,	,	,
cord-347472-n6811ens	99	8	assays	assay	NNS
cord-347472-n6811ens	99	9	should	should	MD
cord-347472-n6811ens	99	10	be	be	VB
cord-347472-n6811ens	99	11	designed	design	VBN
cord-347472-n6811ens	99	12	and	and	CC
cord-347472-n6811ens	99	13	updated	update	VBN
cord-347472-n6811ens	99	14	to	to	TO
cord-347472-n6811ens	99	15	strive	strive	VB
cord-347472-n6811ens	99	16	for	for	IN
cord-347472-n6811ens	99	17	maximum	maximum	JJ
cord-347472-n6811ens	99	18	specificity	specificity	NN
cord-347472-n6811ens	99	19	.	.	.
cord-347472-n6811ens	100	1	The	the	DT
cord-347472-n6811ens	100	2	cost	cost	NN
cord-347472-n6811ens	100	3	of	of	IN
cord-347472-n6811ens	100	4	incorrectly	incorrectly	RB
cord-347472-n6811ens	100	5	clearing	clear	VBG
cord-347472-n6811ens	100	6	SARS	SARS	NNP
cord-347472-n6811ens	100	7	-	-	HYPH
cord-347472-n6811ens	100	8	CoV-2-positive	cov-2-positive	JJ
cord-347472-n6811ens	100	9	patients	patient	NNS
cord-347472-n6811ens	100	10	is	be	VBZ
cord-347472-n6811ens	100	11	incalculably	incalculably	RB
cord-347472-n6811ens	100	12	large	large	JJ
cord-347472-n6811ens	100	13	.	.	.
cord-347472-n6811ens	101	1	Implementing	implement	VBG
cord-347472-n6811ens	101	2	a	a	DT
cord-347472-n6811ens	101	3	redesigned	redesign	VBN
cord-347472-n6811ens	101	4	control	control	NN
cord-347472-n6811ens	101	5	that	that	WDT
cord-347472-n6811ens	101	6	performs	perform	VBZ
cord-347472-n6811ens	101	7	as	as	IN
cord-347472-n6811ens	101	8	intended	intend	VBN
cord-347472-n6811ens	101	9	amounts	amount	NNS
cord-347472-n6811ens	101	10	to	to	IN
cord-347472-n6811ens	101	11	pennies	penny	NNS
cord-347472-n6811ens	101	12	per	per	IN
cord-347472-n6811ens	101	13	test	test	NN
cord-347472-n6811ens	101	14	.	.	.
cord-347472-n6811ens	102	1	A	a	DT
cord-347472-n6811ens	102	2	new	new	JJ
cord-347472-n6811ens	102	3	primer	primer	NN
cord-347472-n6811ens	102	4	/	/	SYM
cord-347472-n6811ens	102	5	probe	probe	NN
cord-347472-n6811ens	102	6	set	set	VBN
cord-347472-n6811ens	102	7	,	,	,
cord-347472-n6811ens	102	8	such	such	JJ
cord-347472-n6811ens	102	9	as	as	IN
cord-347472-n6811ens	102	10	the	the	DT
cord-347472-n6811ens	102	11	one	one	NN
cord-347472-n6811ens	102	12	described	describe	VBN
cord-347472-n6811ens	102	13	here	here	RB
cord-347472-n6811ens	102	14	,	,	,
cord-347472-n6811ens	102	15	is	be	VBZ
cord-347472-n6811ens	102	16	a	a	DT
cord-347472-n6811ens	102	17	drop	drop	NN
cord-347472-n6811ens	102	18	-	-	HYPH
cord-347472-n6811ens	102	19	in	in	RP
cord-347472-n6811ens	102	20	replacement	replacement	NN
cord-347472-n6811ens	102	21	that	that	WDT
cord-347472-n6811ens	102	22	can	can	MD
cord-347472-n6811ens	102	23	be	be	VB
cord-347472-n6811ens	102	24	manufactured	manufacture	VBN
cord-347472-n6811ens	102	25	rapidly	rapidly	RB
cord-347472-n6811ens	102	26	and	and	CC
cord-347472-n6811ens	102	27	at	at	IN
cord-347472-n6811ens	102	28	scale	scale	NN
cord-347472-n6811ens	102	29	.	.	.
cord-347472-n6811ens	103	1	It	-PRON-	PRP
cord-347472-n6811ens	103	2	is	be	VBZ
cord-347472-n6811ens	103	3	imperative	imperative	JJ
cord-347472-n6811ens	103	4	that	that	IN
cord-347472-n6811ens	103	5	the	the	DT
cord-347472-n6811ens	103	6	CDC	CDC	NNP
cord-347472-n6811ens	103	7	take	take	VB
cord-347472-n6811ens	103	8	action	action	NN
cord-347472-n6811ens	103	9	to	to	TO
cord-347472-n6811ens	103	10	update	update	VB
cord-347472-n6811ens	103	11	its	-PRON-	PRP$
cord-347472-n6811ens	103	12	clinical	clinical	JJ
cord-347472-n6811ens	103	13	algorithms	algorithm	NNS
cord-347472-n6811ens	103	14	and	and	CC
cord-347472-n6811ens	103	15	specify	specify	VB
cord-347472-n6811ens	103	16	a	a	DT
cord-347472-n6811ens	103	17	re	re	NN
cord-347472-n6811ens	103	18	-	-	JJ
cord-347472-n6811ens	103	19	designed	design	VBN
cord-347472-n6811ens	103	20	transcript	transcript	NN
cord-347472-n6811ens	103	21	-	-	HYPH
cord-347472-n6811ens	103	22	specific	specific	JJ
cord-347472-n6811ens	103	23	extraction	extraction	NN
cord-347472-n6811ens	103	24	control	control	NN
cord-347472-n6811ens	103	25	that	that	WDT
cord-347472-n6811ens	103	26	performs	perform	VBZ
cord-347472-n6811ens	103	27	all	all	DT
cord-347472-n6811ens	103	28	of	of	IN
cord-347472-n6811ens	103	29	its	-PRON-	PRP$
cord-347472-n6811ens	103	30	claimed	claim	VBN
cord-347472-n6811ens	103	31	,	,	,
cord-347472-n6811ens	103	32	and	and	CC
cord-347472-n6811ens	103	33	critically	critically	RB
cord-347472-n6811ens	103	34	important	important	JJ
cord-347472-n6811ens	103	35	,	,	,
cord-347472-n6811ens	103	36	functions	function	NNS
cord-347472-n6811ens	103	37	.	.	.
cord-347472-n6811ens	104	1	5	5	LS
cord-347472-n6811ens	104	2	.	.	.
cord-347472-n6811ens	105	1	The	the	DT
cord-347472-n6811ens	105	2	amount	amount	NN
cord-347472-n6811ens	105	3	of	of	IN
cord-347472-n6811ens	105	4	co	co	JJ
cord-347472-n6811ens	105	5	-	-	JJ
cord-347472-n6811ens	105	6	purified	purify	VBN
cord-347472-n6811ens	105	7	DNA	dna	NN
cord-347472-n6811ens	105	8	is	be	VBZ
cord-347472-n6811ens	105	9	lower	low	JJR
cord-347472-n6811ens	105	10	for	for	IN
cord-347472-n6811ens	105	11	Trizol	Trizol	NNP
cord-347472-n6811ens	105	12	-	-	HYPH
cord-347472-n6811ens	105	13	purified	purify	VBN
cord-347472-n6811ens	105	14	samples	sample	NNS
cord-347472-n6811ens	105	15	despite	despite	IN
cord-347472-n6811ens	105	16	use	use	NN
cord-347472-n6811ens	105	17	of	of	IN
cord-347472-n6811ens	105	18	a	a	DT
cord-347472-n6811ens	105	19	larger	large	JJR
cord-347472-n6811ens	105	20	fraction	fraction	NN
cord-347472-n6811ens	105	21	of	of	IN
cord-347472-n6811ens	105	22	the	the	DT
cord-347472-n6811ens	105	23	sample	sample	NN
cord-347472-n6811ens	105	24	.	.	.
cord-347472-n6811ens	106	1	This	this	DT
cord-347472-n6811ens	106	2	may	may	MD
cord-347472-n6811ens	106	3	reflect	reflect	VB
cord-347472-n6811ens	106	4	an	an	DT
cord-347472-n6811ens	106	5	even	even	RB
cord-347472-n6811ens	106	6	greater	great	JJR
cord-347472-n6811ens	106	7	RNA	RNA	NNP
cord-347472-n6811ens	106	8	:	:	:
cord-347472-n6811ens	106	9	DNA	dna	NN
cord-347472-n6811ens	106	10	co	co	NN
cord-347472-n6811ens	106	11	-	-	JJ
cord-347472-n6811ens	106	12	purification	purification	JJ
cord-347472-n6811ens	106	13	ratio	ratio	NN
cord-347472-n6811ens	106	14	but	but	CC
cord-347472-n6811ens	106	15	may	may	MD
cord-347472-n6811ens	106	16	also	also	RB
cord-347472-n6811ens	106	17	reflect	reflect	VB
cord-347472-n6811ens	106	18	evolving	evolve	VBG
cord-347472-n6811ens	106	19	clinical	clinical	JJ
cord-347472-n6811ens	106	20	skills	skill	NNS
cord-347472-n6811ens	106	21	in	in	IN
cord-347472-n6811ens	106	22	patient	patient	JJ
cord-347472-n6811ens	106	23	sample	sample	NN
cord-347472-n6811ens	106	24	collection	collection	NN
cord-347472-n6811ens	106	25	during	during	IN
cord-347472-n6811ens	106	26	COVID-19	covid-19	JJ
cord-347472-n6811ens	106	27	testing	testing	NN
cord-347472-n6811ens	106	28	.	.	.
cord-347472-n6811ens	107	1	6	6	LS
cord-347472-n6811ens	107	2	.	.	.
cord-347472-n6811ens	108	1	The	the	DT
cord-347472-n6811ens	108	2	CDC	CDC	NNP
cord-347472-n6811ens	108	3	EUA	EUA	NNP
cord-347472-n6811ens	108	4	states	state	VBZ
cord-347472-n6811ens	108	5	that	that	IN
cord-347472-n6811ens	108	6	the	the	DT
cord-347472-n6811ens	108	7	extraction	extraction	NN
cord-347472-n6811ens	108	8	control	control	NN
cord-347472-n6811ens	108	9	is	be	VBZ
cord-347472-n6811ens	108	10	considered	consider	VBN
cord-347472-n6811ens	108	11	positive	positive	JJ
cord-347472-n6811ens	108	12	/	/	,
cord-347472-n6811ens	108	13	indicative	indicative	JJ
cord-347472-n6811ens	108	14	of	of	IN
cord-347472-n6811ens	108	15	a	a	DT
cord-347472-n6811ens	108	16	successful	successful	JJ
cord-347472-n6811ens	108	17	extraction	extraction	NN
cord-347472-n6811ens	108	18	,	,	,
cord-347472-n6811ens	108	19	where	where	WRB
cord-347472-n6811ens	108	20	control	control	NN
cord-347472-n6811ens	108	21	probe	probe	VB
cord-347472-n6811ens	108	22	Ct	Ct	NNP
cord-347472-n6811ens	108	23	<	<	XX
cord-347472-n6811ens	108	24	40	40	CD
cord-347472-n6811ens	108	25	cycles	cycle	NNS
cord-347472-n6811ens	108	26	using	use	VBG
cord-347472-n6811ens	108	27	a	a	DT
cord-347472-n6811ens	108	28	7500FASTDx	7500fastdx	JJ
cord-347472-n6811ens	108	29	instrument	instrument	NN
cord-347472-n6811ens	108	30	(	(	-LRB-
cord-347472-n6811ens	108	31	see	see	VB
cord-347472-n6811ens	108	32	Supplementary	Supplementary	NNP
cord-347472-n6811ens	108	33	Text	Text	NNP
cord-347472-n6811ens	108	34	)	)	-RRB-
cord-347472-n6811ens	108	35	.	.	.
cord-347472-n6811ens	109	1	7	7	CD
cord-347472-n6811ens	109	2	.	.	.
cord-347472-n6811ens	110	1	Multiple	multiple	JJ
cord-347472-n6811ens	110	2	vendors	vendor	NNS
cord-347472-n6811ens	110	3	of	of	IN
cord-347472-n6811ens	110	4	purified	purify	VBN
cord-347472-n6811ens	110	5	"	"	``
cord-347472-n6811ens	110	6	reference	reference	NN
cord-347472-n6811ens	110	7	"	"	''
cord-347472-n6811ens	110	8	DNA	dna	NN
cord-347472-n6811ens	110	9	were	be	VBD
cord-347472-n6811ens	110	10	used	use	VBN
cord-347472-n6811ens	110	11	with	with	IN
cord-347472-n6811ens	110	12	similar	similar	JJ
cord-347472-n6811ens	110	13	results	result	NNS
cord-347472-n6811ens	110	14	.	.	.
cord-347472-n6811ens	111	1	Promega	Promega	NNP
cord-347472-n6811ens	111	2	Human	Human	NNP
cord-347472-n6811ens	111	3	Male	Male	NNP
cord-347472-n6811ens	111	4	control	control	NN
cord-347472-n6811ens	111	5	DNA	dna	NN
cord-347472-n6811ens	111	6	is	be	VBZ
cord-347472-n6811ens	111	7	available	available	JJ
cord-347472-n6811ens	111	8	in	in	IN
cord-347472-n6811ens	111	9	relatively	relatively	RB
cord-347472-n6811ens	111	10	large	large	JJ
cord-347472-n6811ens	111	11	lot	lot	NN
cord-347472-n6811ens	111	12	-	-	HYPH
cord-347472-n6811ens	111	13	controlled	control	VBN
cord-347472-n6811ens	111	14	batches	batch	NNS
cord-347472-n6811ens	111	15	,	,	,
cord-347472-n6811ens	111	16	validated	validate	VBN
cord-347472-n6811ens	111	17	,	,	,
cord-347472-n6811ens	111	18	is	be	VBZ
cord-347472-n6811ens	111	19	widely	widely	RB
cord-347472-n6811ens	111	20	cited	cite	VBN
cord-347472-n6811ens	111	21	,	,	,
cord-347472-n6811ens	111	22	and	and	CC
cord-347472-n6811ens	111	23	is	be	VBZ
cord-347472-n6811ens	111	24	used	use	VBN
cord-347472-n6811ens	111	25	in	in	IN
cord-347472-n6811ens	111	26	all	all	DT
cord-347472-n6811ens	111	27	figures	figure	NNS
cord-347472-n6811ens	111	28	shown	show	VBN
cord-347472-n6811ens	111	29	here	here	RB
cord-347472-n6811ens	111	30	.	.	.
cord-347472-n6811ens	112	1	8	8	CD
cord-347472-n6811ens	112	2	.	.	.
cord-347472-n6811ens	113	1	Nasopharyngeal	Nasopharyngeal	NNP
cord-347472-n6811ens	113	2	(	(	-LRB-
cord-347472-n6811ens	113	3	NP	NP	NNP
cord-347472-n6811ens	113	4	)	)	-RRB-
cord-347472-n6811ens	113	5	swabs	swab	NNS
cord-347472-n6811ens	113	6	in	in	IN
cord-347472-n6811ens	113	7	transport	transport	NN
cord-347472-n6811ens	113	8	media	medium	NNS
cord-347472-n6811ens	113	9	are	be	VBP
cord-347472-n6811ens	113	10	a	a	DT
cord-347472-n6811ens	113	11	particularly	particularly	RB
cord-347472-n6811ens	113	12	sanguineous	sanguineous	JJ
cord-347472-n6811ens	113	13	mixture	mixture	NN
cord-347472-n6811ens	113	14	that	that	WDT
cord-347472-n6811ens	113	15	are	be	VBP
cord-347472-n6811ens	113	16	frequently	frequently	RB
cord-347472-n6811ens	113	17	received	receive	VBN
cord-347472-n6811ens	113	18	with	with	IN
cord-347472-n6811ens	113	19	co	co	JJ
cord-347472-n6811ens	113	20	-	-	JJ
cord-347472-n6811ens	113	21	collected	collect	VBN
cord-347472-n6811ens	113	22	patient	patient	JJ
cord-347472-n6811ens	113	23	cells	cell	NNS
cord-347472-n6811ens	113	24	and	and	CC
cord-347472-n6811ens	113	25	tissue	tissue	NN
cord-347472-n6811ens	113	26	.	.	.
cord-347472-n6811ens	114	1	While	while	IN
cord-347472-n6811ens	114	2	there	there	EX
cord-347472-n6811ens	114	3	may	may	MD
cord-347472-n6811ens	114	4	be	be	VB
cord-347472-n6811ens	114	5	more	more	RBR
cord-347472-n6811ens	114	6	patient	patient	JJ
cord-347472-n6811ens	114	7	DNA	dna	NN
cord-347472-n6811ens	114	8	in	in	IN
cord-347472-n6811ens	114	9	NP	NP	NNP
cord-347472-n6811ens	114	10	-	-	HYPH
cord-347472-n6811ens	114	11	derived	derive	VBN
cord-347472-n6811ens	114	12	specimens	specimen	NNS
cord-347472-n6811ens	114	13	than	than	IN
cord-347472-n6811ens	114	14	other	other	JJ
cord-347472-n6811ens	114	15	collection	collection	NN
cord-347472-n6811ens	114	16	types	type	NNS
cord-347472-n6811ens	114	17	,	,	,
cord-347472-n6811ens	114	18	human	human	JJ
cord-347472-n6811ens	114	19	genomic	genomic	JJ
cord-347472-n6811ens	114	20	DNA	dna	NN
cord-347472-n6811ens	114	21	is	be	VBZ
cord-347472-n6811ens	114	22	routinely	routinely	RB
cord-347472-n6811ens	114	23	intentionally	intentionally	RB
cord-347472-n6811ens	114	24	collected	collect	VBN
cord-347472-n6811ens	114	25	from	from	IN
cord-347472-n6811ens	114	26	saliva	saliva	NN
cord-347472-n6811ens	114	27	,	,	,
cord-347472-n6811ens	114	28	sputum	sputum	NN
cord-347472-n6811ens	114	29	,	,	,
cord-347472-n6811ens	114	30	and	and	CC
cord-347472-n6811ens	114	31	other	other	JJ
cord-347472-n6811ens	114	32	COVID-19	COVID-19	NNP
cord-347472-n6811ens	114	33	relevant	relevant	JJ
cord-347472-n6811ens	114	34	anatomic	anatomic	JJ
cord-347472-n6811ens	114	35	sites	site	NNS
cord-347472-n6811ens	114	36	.	.	.
cord-347472-n6811ens	115	1	9	9	LS
cord-347472-n6811ens	115	2	.	.	.
cord-347472-n6811ens	116	1	A	a	DT
cord-347472-n6811ens	116	2	properly	properly	RB
cord-347472-n6811ens	116	3	-	-	HYPH
cord-347472-n6811ens	116	4	processed	process	VBN
cord-347472-n6811ens	116	5	blank	blank	JJ
cord-347472-n6811ens	116	6	swab	swab	NN
cord-347472-n6811ens	116	7	or	or	CC
cord-347472-n6811ens	116	8	empty	empty	JJ
cord-347472-n6811ens	116	9	vial	vial	NN
cord-347472-n6811ens	116	10	of	of	IN
cord-347472-n6811ens	116	11	VTM	VTM	NNP
cord-347472-n6811ens	116	12	will	will	MD
cord-347472-n6811ens	116	13	generate	generate	VB
cord-347472-n6811ens	116	14	,	,	,
cord-347472-n6811ens	116	15	as	as	IN
cord-347472-n6811ens	116	16	intended	intend	VBN
cord-347472-n6811ens	116	17	,	,	,
cord-347472-n6811ens	116	18	a	a	DT
cord-347472-n6811ens	116	19	spikein	spikein	NNP
cord-347472-n6811ens	116	20	positive	positive	JJ
cord-347472-n6811ens	116	21	signal	signal	NN
cord-347472-n6811ens	116	22	.	.	.
cord-347472-n6811ens	116	23	 	 	_SP
cord-347472-n6811ens	117	1	Figures	figure	NNS
cord-347472-n6811ens	118	1	S1-S3	S1-S3	NNP
cord-347472-n6811ens	119	1	Table	table	NN
cord-347472-n6811ens	119	2	S1	S1	NNP
cord-347472-n6811ens	119	3	Fig	Fig	NNP
cord-347472-n6811ens	119	4	.	.	.
cord-347472-n6811ens	120	1	1	1	LS
cord-347472-n6811ens	120	2	.	.	.
cord-347472-n6811ens	121	1	A.	A.	NNP
cord-347472-n6811ens	122	1	The	the	DT
cord-347472-n6811ens	122	2	CDC	CDC	NNP
cord-347472-n6811ens	122	3	-	-	HYPH
cord-347472-n6811ens	122	4	specified	specify	VBN
cord-347472-n6811ens	122	5	SARS	SARS	NNP
cord-347472-n6811ens	122	6	-	-	HYPH
cord-347472-n6811ens	122	7	CoV-2	CoV-2	NNP
cord-347472-n6811ens	122	8	qPCR	qpcr	NN
cord-347472-n6811ens	122	9	assay	assay	NN
cord-347472-n6811ens	122	10	and	and	CC
cord-347472-n6811ens	122	11	associated	associate	VBN
cord-347472-n6811ens	122	12	clinical	clinical	JJ
cord-347472-n6811ens	122	13	algorithm	algorithm	NN
cord-347472-n6811ens	122	14	are	be	VBP
cord-347472-n6811ens	122	15	intended	intend	VBN
cord-347472-n6811ens	122	16	to	to	TO
cord-347472-n6811ens	122	17	inform	inform	VB
cord-347472-n6811ens	122	18	for	for	IN
cord-347472-n6811ens	122	19	presence	presence	NN
cord-347472-n6811ens	122	20	of	of	IN
cord-347472-n6811ens	122	21	virus	virus	NN
cord-347472-n6811ens	122	22	,	,	,
cord-347472-n6811ens	122	23	or	or	CC
cord-347472-n6811ens	122	24	that	that	IN
cord-347472-n6811ens	122	25	the	the	DT
cord-347472-n6811ens	122	26	assay	assay	NN
cord-347472-n6811ens	122	27	worked	work	VBD
cord-347472-n6811ens	122	28	and	and	CC
cord-347472-n6811ens	122	29	could	could	MD
cord-347472-n6811ens	122	30	have	have	VB
cord-347472-n6811ens	122	31	found	find	VBN
cord-347472-n6811ens	122	32	virus	virus	NN
cord-347472-n6811ens	122	33	,	,	,
cord-347472-n6811ens	122	34	but	but	CC
cord-347472-n6811ens	122	35	that	that	IN
cord-347472-n6811ens	122	36	no	no	DT
cord-347472-n6811ens	122	37	virus	virus	NN
cord-347472-n6811ens	122	38	was	be	VBD
cord-347472-n6811ens	122	39	found	find	VBN
cord-347472-n6811ens	122	40	.	.	.
cord-347472-n6811ens	123	1	Signal	signal	NN
cord-347472-n6811ens	123	2	from	from	IN
cord-347472-n6811ens	123	3	virus	virus	NN
cord-347472-n6811ens	123	4	-	-	HYPH
cord-347472-n6811ens	123	5	targeting	target	VBG
cord-347472-n6811ens	123	6	RT-	RT-	NNS
cord-347472-n6811ens	124	1	qPCRs	qPCRs	NNP
cord-347472-n6811ens	124	2	is	be	VBZ
cord-347472-n6811ens	124	3	sufficient	sufficient	JJ
cord-347472-n6811ens	124	4	to	to	TO
cord-347472-n6811ens	124	5	report	report	VB
cord-347472-n6811ens	124	6	"	"	``
cord-347472-n6811ens	124	7	Positive	positive	JJ
cord-347472-n6811ens	124	8	2019-nCoV	2019-ncov	CD
cord-347472-n6811ens	124	9	"	"	''
cord-347472-n6811ens	124	10	.	.	.
cord-347472-n6811ens	125	1	An	an	DT
cord-347472-n6811ens	125	2	"	"	``
cord-347472-n6811ens	125	3	invalid	invalid	JJ
cord-347472-n6811ens	125	4	"	"	''
cord-347472-n6811ens	125	5	report	report	NN
cord-347472-n6811ens	125	6	is	be	VBZ
cord-347472-n6811ens	125	7	returned	return	VBN
cord-347472-n6811ens	125	8	where	where	WRB
cord-347472-n6811ens	125	9	all	all	DT
cord-347472-n6811ens	125	10	viral	viral	JJ
cord-347472-n6811ens	125	11	and	and	CC
cord-347472-n6811ens	125	12	control	control	NN
cord-347472-n6811ens	125	13	probes	probe	NNS
cord-347472-n6811ens	125	14	are	be	VBP
cord-347472-n6811ens	125	15	negative	negative	JJ
cord-347472-n6811ens	125	16	.	.	.
cord-347472-n6811ens	126	1	The	the	DT
cord-347472-n6811ens	126	2	clinical	clinical	JJ
cord-347472-n6811ens	126	3	interpretation	interpretation	NN
cord-347472-n6811ens	126	4	is	be	VBZ
cord-347472-n6811ens	126	5	"	"	``
cord-347472-n6811ens	126	6	2019-nCoV	2019-ncov	CD
cord-347472-n6811ens	126	7	not	not	RB
cord-347472-n6811ens	126	8	detected	detect	VBN
cord-347472-n6811ens	126	9	"	"	''
cord-347472-n6811ens	126	10	when	when	WRB
cord-347472-n6811ens	126	11	a	a	DT
cord-347472-n6811ens	126	12	human	human	JJ
cord-347472-n6811ens	126	13	control	control	NN
cord-347472-n6811ens	126	14	,	,	,
cord-347472-n6811ens	126	15	targeting	target	VBG
cord-347472-n6811ens	126	16	the	the	DT
cord-347472-n6811ens	126	17	RPP30	RPP30	NNP
cord-347472-n6811ens	126	18	gene	gene	NN
cord-347472-n6811ens	126	19	,	,	,
cord-347472-n6811ens	126	20	returns	return	VBZ
cord-347472-n6811ens	126	21	a	a	DT
cord-347472-n6811ens	126	22	positive	positive	JJ
cord-347472-n6811ens	126	23	signal	signal	NN
cord-347472-n6811ens	126	24	and	and	CC
cord-347472-n6811ens	126	25	when	when	WRB
cord-347472-n6811ens	126	26	viral	viral	JJ
cord-347472-n6811ens	126	27	probes	probe	NNS
cord-347472-n6811ens	126	28	are	be	VBP
cord-347472-n6811ens	126	29	negative	negative	JJ
cord-347472-n6811ens	126	30	.	.	.
cord-347472-n6811ens	127	1	Patients	patient	NNS
cord-347472-n6811ens	127	2	who	who	WP
cord-347472-n6811ens	127	3	are	be	VBP
cord-347472-n6811ens	127	4	truly	truly	RB
cord-347472-n6811ens	127	5	virus	virus	NN
cord-347472-n6811ens	127	6	negative	negative	JJ
cord-347472-n6811ens	127	7	will	will	MD
cord-347472-n6811ens	127	8	fall	fall	VB
cord-347472-n6811ens	127	9	into	into	IN
cord-347472-n6811ens	127	10	this	this	DT
cord-347472-n6811ens	127	11	category	category	NN
cord-347472-n6811ens	127	12	.	.	.
cord-347472-n6811ens	128	1	So	so	RB
cord-347472-n6811ens	128	2	will	will	MD
cord-347472-n6811ens	128	3	patients	patient	NNS
cord-347472-n6811ens	128	4	for	for	IN
cord-347472-n6811ens	128	5	whom	whom	WP
cord-347472-n6811ens	128	6	sample	sample	NN
cord-347472-n6811ens	128	7	collection	collection	NN
cord-347472-n6811ens	128	8	,	,	,
cord-347472-n6811ens	128	9	handling	handling	NN
cord-347472-n6811ens	128	10	,	,	,
cord-347472-n6811ens	128	11	or	or	CC
cord-347472-n6811ens	128	12	analysis	analysis	NN
cord-347472-n6811ens	128	13	problems	problem	NNS
cord-347472-n6811ens	128	14	have	have	VBP
cord-347472-n6811ens	128	15	occurred	occur	VBN
cord-347472-n6811ens	128	16	,	,	,
cord-347472-n6811ens	128	17	leading	lead	VBG
cord-347472-n6811ens	128	18	to	to	IN
cord-347472-n6811ens	128	19	false	false	JJ
cord-347472-n6811ens	128	20	negative	negative	JJ
cord-347472-n6811ens	128	21	results	result	NNS
cord-347472-n6811ens	128	22	.	.	.
cord-347472-n6811ens	129	1	B.	B.	NNP
cord-347472-n6811ens	130	1	The	the	DT
cord-347472-n6811ens	130	2	CDC	CDC	NNP
cord-347472-n6811ens	130	3	-	-	HYPH
cord-347472-n6811ens	130	4	specified	specify	VBN
cord-347472-n6811ens	130	5	extraction	extraction	NN
cord-347472-n6811ens	130	6	control	control	NN
cord-347472-n6811ens	130	7	primers	primer	NNS
cord-347472-n6811ens	130	8	and	and	CC
cord-347472-n6811ens	130	9	probe	probe	NN
cord-347472-n6811ens	130	10	recognize	recognize	VB
cord-347472-n6811ens	130	11	a	a	DT
cord-347472-n6811ens	130	12	sequence	sequence	NN
cord-347472-n6811ens	130	13	that	that	WDT
cord-347472-n6811ens	130	14	is	be	VBZ
cord-347472-n6811ens	130	15	identical	identical	JJ
cord-347472-n6811ens	130	16	in	in	IN
cord-347472-n6811ens	130	17	reverse	reverse	NN
cord-347472-n6811ens	130	18	transcribed	transcribe	VBN
cord-347472-n6811ens	130	19	RNA	RNA	NNP
cord-347472-n6811ens	130	20	and	and	CC
cord-347472-n6811ens	130	21	genomic	genomic	JJ
cord-347472-n6811ens	130	22	DNA	dna	NN
cord-347472-n6811ens	130	23	.	.	.
cord-347472-n6811ens	131	1	Megablast	megablast	JJ
cord-347472-n6811ens	131	2	search	search	NN
cord-347472-n6811ens	131	3	of	of	IN
cord-347472-n6811ens	131	4	the	the	DT
cord-347472-n6811ens	131	5	human	human	JJ
cord-347472-n6811ens	131	6	refseq_dna	refseq_dna	NNS
cord-347472-n6811ens	131	7	database	database	NN
cord-347472-n6811ens	131	8	identifies	identify	VBZ
cord-347472-n6811ens	131	9	perfect	perfect	JJ
cord-347472-n6811ens	131	10	matches	match	NNS
cord-347472-n6811ens	131	11	for	for	IN
cord-347472-n6811ens	131	12	the	the	DT
cord-347472-n6811ens	131	13	CDC	CDC	NNP
cord-347472-n6811ens	131	14	-	-	HYPH
cord-347472-n6811ens	131	15	control	control	NN
cord-347472-n6811ens	131	16	primers	primer	NNS
cord-347472-n6811ens	131	17	,	,	,
cord-347472-n6811ens	131	18	RP	RP	NNP
cord-347472-n6811ens	131	19	-	-	HYPH
cord-347472-n6811ens	131	20	F	F	NNP
cord-347472-n6811ens	131	21	and	and	CC
cord-347472-n6811ens	131	22	RP	RP	NNP
cord-347472-n6811ens	131	23	-	-	HYPH
cord-347472-n6811ens	131	24	R	R	NNP
cord-347472-n6811ens	131	25	,	,	,
cord-347472-n6811ens	131	26	and	and	CC
cord-347472-n6811ens	131	27	probe	probe	NN
cord-347472-n6811ens	131	28	,	,	,
cord-347472-n6811ens	131	29	RP	RP	NNP
cord-347472-n6811ens	132	1	-P.	-P.	NFP
cord-347472-n6811ens	132	2	This	this	DT
cord-347472-n6811ens	132	3	design	design	NN
cord-347472-n6811ens	132	4	amplifies	amplify	VBZ
cord-347472-n6811ens	132	5	and	and	CC
cord-347472-n6811ens	132	6	detects	detect	VBZ
cord-347472-n6811ens	132	7	a	a	DT
cord-347472-n6811ens	132	8	65	65	CD
cord-347472-n6811ens	132	9	base	base	NN
cord-347472-n6811ens	132	10	pair	pair	NN
cord-347472-n6811ens	132	11	amplicon	amplicon	NN
cord-347472-n6811ens	132	12	located	locate	VBN
cord-347472-n6811ens	132	13	entirely	entirely	RB
cord-347472-n6811ens	132	14	within	within	IN
cord-347472-n6811ens	132	15	exon	exon	NN
cord-347472-n6811ens	132	16	1	1	CD
cord-347472-n6811ens	132	17	of	of	IN
cord-347472-n6811ens	132	18	the	the	DT
cord-347472-n6811ens	132	19	RPP30	RPP30	NNP
cord-347472-n6811ens	132	20	gene	gene	NN
cord-347472-n6811ens	132	21	,	,	,
cord-347472-n6811ens	132	22	located	locate	VBN
cord-347472-n6811ens	132	23	on	on	IN
cord-347472-n6811ens	132	24	human	human	JJ
cord-347472-n6811ens	132	25	chromosome	chromosome	NN
cord-347472-n6811ens	132	26	10	10	CD
cord-347472-n6811ens	132	27	(	(	-LRB-
cord-347472-n6811ens	132	28	coordinates	coordinate	NNS
cord-347472-n6811ens	132	29	from	from	IN
cord-347472-n6811ens	132	30	hg38	hg38	NNP
cord-347472-n6811ens	132	31	human	human	JJ
cord-347472-n6811ens	132	32	genome	genome	NN
cord-347472-n6811ens	132	33	build	build	NN
cord-347472-n6811ens	132	34	)	)	-RRB-
cord-347472-n6811ens	132	35	.	.	.
cord-347472-n6811ens	133	1	Specific	specific	JJ
cord-347472-n6811ens	133	2	detection	detection	NN
cord-347472-n6811ens	133	3	of	of	IN
cord-347472-n6811ens	133	4	reverse	reverse	RB
cord-347472-n6811ens	133	5	-	-	HYPH
cord-347472-n6811ens	133	6	transcribed	transcribe	VBN
cord-347472-n6811ens	133	7	RNAs	rna	NNS
cord-347472-n6811ens	133	8	can	can	MD
cord-347472-n6811ens	133	9	be	be	VB
cord-347472-n6811ens	133	10	accomplished	accomplish	VBN
cord-347472-n6811ens	133	11	by	by	IN
cord-347472-n6811ens	133	12	design	design	NN
cord-347472-n6811ens	133	13	of	of	IN
cord-347472-n6811ens	133	14	an	an	DT
cord-347472-n6811ens	133	15	exon	exon	NN
cord-347472-n6811ens	133	16	-	-	HYPH
cord-347472-n6811ens	133	17	exon	exon	NN
cord-347472-n6811ens	133	18	junction	junction	NN
cord-347472-n6811ens	133	19	spanning	span	VBG
cord-347472-n6811ens	133	20	amplicon	amplicon	NN
cord-347472-n6811ens	133	21	and	and	CC
cord-347472-n6811ens	133	22	probe	probe	NN
cord-347472-n6811ens	133	23	(	(	-LRB-
cord-347472-n6811ens	133	24	Supplemental	Supplemental	NNP
cord-347472-n6811ens	133	25	Figure	Figure	NNP
cord-347472-n6811ens	133	26	3	3	CD
cord-347472-n6811ens	133	27	)	)	-RRB-
cord-347472-n6811ens	133	28	.	.	.
cord-347472-n6811ens	134	1	Genomic	genomic	JJ
cord-347472-n6811ens	134	2	DNA	dna	NN
cord-347472-n6811ens	134	3	is	be	VBZ
cord-347472-n6811ens	134	4	co	co	VBN
cord-347472-n6811ens	134	5	-	-	JJ
cord-347472-n6811ens	134	6	purified	purify	VBN
cord-347472-n6811ens	135	1	during	during	IN
cord-347472-n6811ens	135	2	RNA	RNA	NNP
cord-347472-n6811ens	135	3	extraction	extraction	NN
cord-347472-n6811ens	135	4	of	of	IN
cord-347472-n6811ens	135	5	clinical	clinical	JJ
cord-347472-n6811ens	135	6	COVID-19	covid-19	NN
cord-347472-n6811ens	135	7	samples	sample	NNS
cord-347472-n6811ens	135	8	and	and	CC
cord-347472-n6811ens	135	9	is	be	VBZ
cord-347472-n6811ens	135	10	sufficient	sufficient	JJ
cord-347472-n6811ens	135	11	to	to	TO
cord-347472-n6811ens	135	12	generate	generate	VB
cord-347472-n6811ens	135	13	a	a	DT
cord-347472-n6811ens	135	14	positive	positive	JJ
cord-347472-n6811ens	135	15	signal	signal	NN
cord-347472-n6811ens	135	16	using	use	VBG
cord-347472-n6811ens	135	17	the	the	DT
cord-347472-n6811ens	135	18	CDC	CDC	NNP
cord-347472-n6811ens	135	19	extraction	extraction	NN
cord-347472-n6811ens	135	20	control	control	NN
cord-347472-n6811ens	135	21	.	.	.
cord-347472-n6811ens	136	1	A.	A.	NNP
cord-347472-n6811ens	136	2	qPCR	qPCR	NNP
cord-347472-n6811ens	136	3	(	(	-LRB-
cord-347472-n6811ens	136	4	without	without	IN
cord-347472-n6811ens	136	5	reverse	reverse	JJ
cord-347472-n6811ens	136	6	transcription	transcription	NN
cord-347472-n6811ens	136	7	)	)	-RRB-
cord-347472-n6811ens	136	8	was	be	VBD
cord-347472-n6811ens	136	9	performed	perform	VBN
cord-347472-n6811ens	136	10	using	use	VBG
cord-347472-n6811ens	136	11	the	the	DT
cord-347472-n6811ens	136	12	CDC	CDC	NNP
cord-347472-n6811ens	136	13	extraction	extraction	NN
cord-347472-n6811ens	136	14	control	control	NN
cord-347472-n6811ens	136	15	(	(	-LRB-
cord-347472-n6811ens	136	16	RP	RP	NNP
cord-347472-n6811ens	136	17	)	)	-RRB-
cord-347472-n6811ens	136	18	probe	probe	NN
cord-347472-n6811ens	136	19	.	.	.
cord-347472-n6811ens	137	1	Raw	raw	JJ
cord-347472-n6811ens	137	2	Ct	Ct	NNP
cord-347472-n6811ens	137	3	values	value	NNS
cord-347472-n6811ens	137	4	are	be	VBP
cord-347472-n6811ens	137	5	shown	show	VBN
cord-347472-n6811ens	137	6	.	.	.
cord-347472-n6811ens	138	1	A	a	DT
cord-347472-n6811ens	138	2	total	total	NN
cord-347472-n6811ens	138	3	of	of	IN
cord-347472-n6811ens	138	4	28	28	CD
cord-347472-n6811ens	138	5	representative	representative	NN
cord-347472-n6811ens	138	6	COVID-19	covid-19	CD
cord-347472-n6811ens	138	7	positive	positive	JJ
cord-347472-n6811ens	138	8	clinical	clinical	JJ
cord-347472-n6811ens	138	9	samples	sample	NNS
cord-347472-n6811ens	138	10	were	be	VBD
cord-347472-n6811ens	138	11	tested	test	VBN
cord-347472-n6811ens	138	12	,	,	,
cord-347472-n6811ens	138	13	fourteen	fourteen	CD
cord-347472-n6811ens	138	14	purified	purify	VBN
cord-347472-n6811ens	138	15	by	by	IN
cord-347472-n6811ens	138	16	automated	automate	VBN
cord-347472-n6811ens	138	17	silica	silica	NN
cord-347472-n6811ens	138	18	spin	spin	NN
cord-347472-n6811ens	138	19	-	-	HYPH
cord-347472-n6811ens	138	20	column	column	NN
cord-347472-n6811ens	138	21	(	(	-LRB-
cord-347472-n6811ens	138	22	Qiagen	Qiagen	NNP
cord-347472-n6811ens	138	23	)	)	-RRB-
cord-347472-n6811ens	138	24	and	and	CC
cord-347472-n6811ens	138	25	fourteen	fourteen	CD
cord-347472-n6811ens	138	26	by	by	IN
cord-347472-n6811ens	138	27	manual	manual	JJ
cord-347472-n6811ens	138	28	organic	organic	JJ
cord-347472-n6811ens	138	29	extractions	extraction	NNS
cord-347472-n6811ens	138	30	(	(	-LRB-
cord-347472-n6811ens	138	31	Trizol	Trizol	NNP
cord-347472-n6811ens	138	32	,	,	,
cord-347472-n6811ens	138	33	Materials	Materials	NNPS
cord-347472-n6811ens	138	34	and	and	CC
cord-347472-n6811ens	138	35	Methods	Methods	NNPS
cord-347472-n6811ens	138	36	)	)	-RRB-
cord-347472-n6811ens	138	37	.	.	.
cord-347472-n6811ens	139	1	All	all	DT
cord-347472-n6811ens	139	2	samples	sample	NNS
cord-347472-n6811ens	139	3	contained	contain	VBD
cord-347472-n6811ens	139	4	genomic	genomic	JJ
cord-347472-n6811ens	139	5	DNA	dna	NN
cord-347472-n6811ens	139	6	and	and	CC
cord-347472-n6811ens	139	7	generated	generate	VBD
cord-347472-n6811ens	139	8	extraction	extraction	NN
cord-347472-n6811ens	139	9	control	control	NN
cord-347472-n6811ens	139	10	positive	positive	JJ
cord-347472-n6811ens	139	11	signals	signal	NNS
cord-347472-n6811ens	139	12	well	well	RB
cord-347472-n6811ens	139	13	above	above	IN
cord-347472-n6811ens	139	14	the	the	DT
cord-347472-n6811ens	139	15	CDC	CDC	NNP
cord-347472-n6811ens	139	16	assay	assay	NN
cord-347472-n6811ens	139	17	threshold	threshold	NN
cord-347472-n6811ens	139	18	(	(	-LRB-
cord-347472-n6811ens	139	19	40	40	CD
cord-347472-n6811ens	139	20	cycles	cycle	NNS
cord-347472-n6811ens	139	21	,	,	,
cord-347472-n6811ens	139	22	horizontal	horizontal	JJ
cord-347472-n6811ens	139	23	dashed	dash	VBN
cord-347472-n6811ens	139	24	line	line	NN
cord-347472-n6811ens	139	25	)	)	-RRB-
cord-347472-n6811ens	139	26	.	.	.
cord-347472-n6811ens	140	1	Significantly	significantly	RB
cord-347472-n6811ens	140	2	more	more	RBR
cord-347472-n6811ens	140	3	genomic	genomic	JJ
cord-347472-n6811ens	140	4	DNA	dna	NN
cord-347472-n6811ens	140	5	was	be	VBD
cord-347472-n6811ens	140	6	present	present	JJ
cord-347472-n6811ens	140	7	in	in	IN
cord-347472-n6811ens	140	8	samples	sample	NNS
cord-347472-n6811ens	140	9	purified	purify	VBN
cord-347472-n6811ens	140	10	by	by	IN
cord-347472-n6811ens	140	11	silica	silica	NN
cord-347472-n6811ens	140	12	spin	spin	NN
cord-347472-n6811ens	140	13	-	-	HYPH
cord-347472-n6811ens	140	14	columns	column	NNS
cord-347472-n6811ens	140	15	compared	compare	VBN
cord-347472-n6811ens	140	16	to	to	IN
cord-347472-n6811ens	140	17	organic	organic	JJ
cord-347472-n6811ens	140	18	extraction	extraction	NN
cord-347472-n6811ens	140	19	(	(	-LRB-
cord-347472-n6811ens	140	20	mean	mean	NNP
cord-347472-n6811ens	140	21	Ct	Ct	NNP
cord-347472-n6811ens	140	22	of	of	IN
cord-347472-n6811ens	140	23	29.6	29.6	CD
cord-347472-n6811ens	140	24	and	and	CC
cord-347472-n6811ens	140	25	34.3	34.3	CD
cord-347472-n6811ens	140	26	,	,	,
cord-347472-n6811ens	140	27	respectively	respectively	RB
cord-347472-n6811ens	140	28	,	,	,
cord-347472-n6811ens	140	29	p=	p=	NNP
cord-347472-n6811ens	140	30	1.21x10	1.21x10	CD
cord-347472-n6811ens	140	31	-6	-6	NNP
cord-347472-n6811ens	140	32	,	,	,
cord-347472-n6811ens	140	33	unpaired	unpaired	JJ
cord-347472-n6811ens	140	34	t	t	NN
cord-347472-n6811ens	140	35	)	)	-RRB-
cord-347472-n6811ens	140	36	.	.	.
cord-347472-n6811ens	141	1	Co	co	NN
cord-347472-n6811ens	141	2	-	-	NN
cord-347472-n6811ens	141	3	purification	purification	NN
cord-347472-n6811ens	141	4	of	of	IN
cord-347472-n6811ens	141	5	DNA	dna	NN
cord-347472-n6811ens	141	6	is	be	VBZ
cord-347472-n6811ens	141	7	to	to	TO
cord-347472-n6811ens	141	8	be	be	VB
cord-347472-n6811ens	141	9	expected	expect	VBN
cord-347472-n6811ens	141	10	based	base	VBN
cord-347472-n6811ens	141	11	on	on	IN
cord-347472-n6811ens	141	12	the	the	DT
cord-347472-n6811ens	141	13	mechanisms	mechanism	NNS
cord-347472-n6811ens	141	14	of	of	IN
cord-347472-n6811ens	141	15	purification	purification	NN
cord-347472-n6811ens	141	16	used	use	VBN
cord-347472-n6811ens	141	17	,	,	,
cord-347472-n6811ens	141	18	and	and	CC
cord-347472-n6811ens	141	19	is	be	VBZ
cord-347472-n6811ens	141	20	openly	openly	RB
cord-347472-n6811ens	141	21	caveated	caveate	VBN
cord-347472-n6811ens	141	22	in	in	IN
cord-347472-n6811ens	141	23	vendor	vendor	NN
cord-347472-n6811ens	141	24	literature	literature	NN
cord-347472-n6811ens	141	25	(	(	-LRB-
cord-347472-n6811ens	141	26	13	13	CD
cord-347472-n6811ens	141	27	)	)	-RRB-
cord-347472-n6811ens	141	28	.	.	.
cord-347472-n6811ens	142	1	Per	per	IN
cord-347472-n6811ens	142	2	-	-	HYPH
cord-347472-n6811ens	142	3	plate	plate	NN
cord-347472-n6811ens	142	4	process	process	NN
cord-347472-n6811ens	142	5	controls	control	NNS
cord-347472-n6811ens	142	6	,	,	,
cord-347472-n6811ens	142	7	shown	show	VBN
cord-347472-n6811ens	142	8	,	,	,
cord-347472-n6811ens	142	9	contained	contain	VBD
cord-347472-n6811ens	142	10	8.6	8.6	CD
cord-347472-n6811ens	142	11	ng	ng	NN
cord-347472-n6811ens	142	12	of	of	IN
cord-347472-n6811ens	142	13	genomic	genomic	JJ
cord-347472-n6811ens	142	14	DNA	dna	NN
cord-347472-n6811ens	142	15	,	,	,
cord-347472-n6811ens	142	16	equivalent	equivalent	JJ
cord-347472-n6811ens	142	17	to	to	IN
cord-347472-n6811ens	142	18	~1250	~1250	XX
cord-347472-n6811ens	142	19	diploid	diploid	JJ
cord-347472-n6811ens	142	20	cells	cell	NNS
cord-347472-n6811ens	142	21	.	.	.
cord-347472-n6811ens	143	1	B.	B.	NNP
cord-347472-n6811ens	143	2	Single	single	JJ
cord-347472-n6811ens	143	3	-	-	HYPH
cord-347472-n6811ens	143	4	cell	cell	NN
cord-347472-n6811ens	143	5	equivalents	equivalent	NNS
cord-347472-n6811ens	143	6	of	of	IN
cord-347472-n6811ens	143	7	purified	purify	VBN
cord-347472-n6811ens	143	8	genomic	genomic	JJ
cord-347472-n6811ens	143	9	DNA	dna	NN
cord-347472-n6811ens	143	10	,	,	,
cord-347472-n6811ens	143	11	absent	absent	JJ
cord-347472-n6811ens	143	12	RNA	RNA	NNP
cord-347472-n6811ens	143	13	,	,	,
cord-347472-n6811ens	143	14	are	be	VBP
cord-347472-n6811ens	143	15	sufficient	sufficient	JJ
cord-347472-n6811ens	143	16	to	to	TO
cord-347472-n6811ens	143	17	generate	generate	VB
cord-347472-n6811ens	143	18	an	an	DT
cord-347472-n6811ens	143	19	"	"	``
cord-347472-n6811ens	143	20	extraction	extraction	NN
cord-347472-n6811ens	143	21	control	control	NN
cord-347472-n6811ens	143	22	positive	positive	JJ
cord-347472-n6811ens	143	23	"	"	``
cord-347472-n6811ens	143	24	signal	signal	NN
cord-347472-n6811ens	143	25	using	use	VBG
cord-347472-n6811ens	143	26	the	the	DT
cord-347472-n6811ens	143	27	CDC	CDC	NNP
cord-347472-n6811ens	143	28	-	-	HYPH
cord-347472-n6811ens	143	29	RP	RP	NNP
cord-347472-n6811ens	143	30	probe	probe	NN
cord-347472-n6811ens	143	31	.	.	.
cord-347472-n6811ens	144	1	RT	RT	NNP
cord-347472-n6811ens	144	2	-	-	HYPH
cord-347472-n6811ens	144	3	qPCR	qPCR	NNP
cord-347472-n6811ens	144	4	was	be	VBD
cord-347472-n6811ens	144	5	performed	perform	VBN
cord-347472-n6811ens	144	6	using	use	VBG
cord-347472-n6811ens	144	7	Viral	Viral	NNP
cord-347472-n6811ens	144	8	One-	One-	NNP
cord-347472-n6811ens	144	9	Step	Step	NNP
cord-347472-n6811ens	144	10	Mastermix	Mastermix	NNP
cord-347472-n6811ens	144	11	(	(	-LRB-
cord-347472-n6811ens	144	12	Materials	material	NNS
cord-347472-n6811ens	144	13	and	and	CC
cord-347472-n6811ens	144	14	Methods	Methods	NNPS
cord-347472-n6811ens	144	15	)	)	-RRB-
cord-347472-n6811ens	144	16	on	on	IN
cord-347472-n6811ens	144	17	2-fold	2-fold	CD
cord-347472-n6811ens	144	18	serial	serial	JJ
cord-347472-n6811ens	144	19	dilutions	dilution	NNS
cord-347472-n6811ens	144	20	of	of	IN
cord-347472-n6811ens	144	21	a	a	DT
cord-347472-n6811ens	144	22	commercially	commercially	RB
cord-347472-n6811ens	144	23	-	-	HYPH
cord-347472-n6811ens	144	24	supplied	supply	VBN
cord-347472-n6811ens	144	25	reference	reference	NN
cord-347472-n6811ens	144	26	human	human	JJ
cord-347472-n6811ens	144	27	DNA	dna	NN
cord-347472-n6811ens	144	28	(	(	-LRB-
cord-347472-n6811ens	144	29	RNA	rna	NN
cord-347472-n6811ens	144	30	-	-	HYPH
cord-347472-n6811ens	144	31	free	free	JJ
cord-347472-n6811ens	144	32	)	)	-RRB-
cord-347472-n6811ens	144	33	.	.	.
cord-347472-n6811ens	145	1	Dilution	dilution	NN
cord-347472-n6811ens	145	2	equivalent	equivalent	JJ
cord-347472-n6811ens	145	3	to	to	IN
cord-347472-n6811ens	145	4	one	one	CD
cord-347472-n6811ens	145	5	diploid	diploid	JJ
cord-347472-n6811ens	145	6	copy	copy	NN
cord-347472-n6811ens	145	7	of	of	IN
cord-347472-n6811ens	145	8	gDNA	gdna	NN
cord-347472-n6811ens	145	9	per	per	IN
cord-347472-n6811ens	145	10	reaction	reaction	NN
cord-347472-n6811ens	145	11	(	(	-LRB-
cord-347472-n6811ens	145	12	6.125pg	6.125pg	CD
cord-347472-n6811ens	145	13	)	)	-RRB-
cord-347472-n6811ens	145	14	generates	generate	VBZ
cord-347472-n6811ens	145	15	signals	signal	NNS
cord-347472-n6811ens	145	16	well	well	RB
cord-347472-n6811ens	145	17	above	above	IN
cord-347472-n6811ens	145	18	the	the	DT
cord-347472-n6811ens	145	19	CDC	CDC	NNP
cord-347472-n6811ens	145	20	-	-	HYPH
cord-347472-n6811ens	145	21	specified	specify	VBN
cord-347472-n6811ens	145	22	threshold	threshold	NN
cord-347472-n6811ens	145	23	,	,	,
cord-347472-n6811ens	145	24	despite	despite	IN
cord-347472-n6811ens	145	25	increase	increase	NN
cord-347472-n6811ens	145	26	in	in	IN
cord-347472-n6811ens	145	27	Ct	Ct	NNP
cord-347472-n6811ens	145	28	variability	variability	NN
cord-347472-n6811ens	145	29	at	at	IN
cord-347472-n6811ens	145	30	extreme	extreme	JJ
cord-347472-n6811ens	145	31	dilutions	dilution	NNS
cord-347472-n6811ens	145	32	.	.	.
cord-347472-n6811ens	146	1	Fig	Fig	NNP
cord-347472-n6811ens	146	2	.	.	.
cord-347472-n6811ens	147	1	3	3	LS
cord-347472-n6811ens	147	2	.	.	.
cord-347472-n6811ens	148	1	CDC	CDC	NNP
cord-347472-n6811ens	148	2	-	-	HYPH
cord-347472-n6811ens	148	3	specified	specify	VBN
cord-347472-n6811ens	148	4	testing	testing	NN
cord-347472-n6811ens	148	5	of	of	IN
cord-347472-n6811ens	148	6	SARS	SARS	NNP
cord-347472-n6811ens	148	7	-	-	HYPH
cord-347472-n6811ens	148	8	CoV-2-positive	cov-2-positive	JJ
cord-347472-n6811ens	148	9	clinical	clinical	JJ
cord-347472-n6811ens	148	10	specimens	specimen	NNS
cord-347472-n6811ens	148	11	return	return	VBP
cord-347472-n6811ens	148	12	a	a	DT
cord-347472-n6811ens	148	13	false	false	JJ
cord-347472-n6811ens	148	14	negative	negative	JJ
cord-347472-n6811ens	148	15	following	following	JJ
cord-347472-n6811ens	148	16	loss	loss	NN
cord-347472-n6811ens	148	17	of	of	IN
cord-347472-n6811ens	148	18	RNA	RNA	NNP
cord-347472-n6811ens	148	19	integrity	integrity	NN
cord-347472-n6811ens	148	20	.	.	.
cord-347472-n6811ens	149	1	Reverse	reverse	JJ
cord-347472-n6811ens	149	2	-	-	HYPH
cord-347472-n6811ens	149	3	transcriptase	transcriptase	NN
cord-347472-n6811ens	149	4	-	-	HYPH
cord-347472-n6811ens	149	5	qPCR	qpcr	NN
cord-347472-n6811ens	149	6	data	datum	NNS
cord-347472-n6811ens	149	7	were	be	VBD
cord-347472-n6811ens	149	8	generated	generate	VBN
cord-347472-n6811ens	149	9	using	use	VBG
cord-347472-n6811ens	149	10	Viral	Viral	NNP
cord-347472-n6811ens	149	11	One	one	CD
cord-347472-n6811ens	149	12	-	-	HYPH
cord-347472-n6811ens	149	13	step	step	NN
cord-347472-n6811ens	149	14	RT	RT	NNP
cord-347472-n6811ens	149	15	-	-	HYPH
cord-347472-n6811ens	149	16	qPCR	qPCR	NNP
cord-347472-n6811ens	149	17	mastermix	mastermix	NNP
cord-347472-n6811ens	149	18	(	(	-LRB-
cord-347472-n6811ens	149	19	Materials	Materials	NNPS
cord-347472-n6811ens	149	20	and	and	CC
cord-347472-n6811ens	149	21	Methods	Methods	NNPS
cord-347472-n6811ens	149	22	)	)	-RRB-
cord-347472-n6811ens	149	23	.	.	.
cord-347472-n6811ens	150	1	Extracted	extract	VBN
cord-347472-n6811ens	150	2	nucleic	nucleic	JJ
cord-347472-n6811ens	150	3	acid	acid	NN
cord-347472-n6811ens	150	4	samples	sample	NNS
cord-347472-n6811ens	150	5	used	use	VBN
cord-347472-n6811ens	150	6	in	in	IN
cord-347472-n6811ens	150	7	Figure	figure	NN
cord-347472-n6811ens	150	8	2	2	CD
cord-347472-n6811ens	150	9	were	be	VBD
cord-347472-n6811ens	150	10	pooled	pool	VBN
cord-347472-n6811ens	150	11	,	,	,
cord-347472-n6811ens	150	12	divided	divide	VBN
cord-347472-n6811ens	150	13	into	into	IN
cord-347472-n6811ens	150	14	aliquots	aliquot	NNS
cord-347472-n6811ens	150	15	,	,	,
cord-347472-n6811ens	150	16	and	and	CC
cord-347472-n6811ens	150	17	treated	treat	VBN
cord-347472-n6811ens	150	18	with	with	IN
cord-347472-n6811ens	150	19	either	either	DT
cord-347472-n6811ens	150	20	nuclease	nuclease	NN
cord-347472-n6811ens	150	21	-	-	HYPH
cord-347472-n6811ens	150	22	free	free	JJ
cord-347472-n6811ens	150	23	water	water	NN
cord-347472-n6811ens	150	24	or	or	CC
cord-347472-n6811ens	150	25	Ribonuclease	Ribonuclease	NNP
cord-347472-n6811ens	150	26	A	A	NNP
cord-347472-n6811ens	150	27	(	(	-LRB-
cord-347472-n6811ens	150	28	RNase	RNase	NNP
cord-347472-n6811ens	150	29	A	A	NNP
cord-347472-n6811ens	150	30	)	)	-RRB-
cord-347472-n6811ens	150	31	.	.	.
cord-347472-n6811ens	151	1	Commercial	Commercial	NNP
cord-347472-n6811ens	151	2	RNA	RNA	NNP
cord-347472-n6811ens	151	3	,	,	,
cord-347472-n6811ens	151	4	DNA	DNA	NNP
cord-347472-n6811ens	151	5	,	,	,
cord-347472-n6811ens	151	6	and	and	CC
cord-347472-n6811ens	151	7	no	no	DT
cord-347472-n6811ens	151	8	-	-	HYPH
cord-347472-n6811ens	151	9	template	template	NN
cord-347472-n6811ens	151	10	controls	control	NNS
cord-347472-n6811ens	151	11	(	(	-LRB-
cord-347472-n6811ens	151	12	NTC	NTC	NNP
cord-347472-n6811ens	151	13	)	)	-RRB-
cord-347472-n6811ens	151	14	were	be	VBD
cord-347472-n6811ens	151	15	treated	treat	VBN
cord-347472-n6811ens	151	16	in	in	IN
cord-347472-n6811ens	151	17	parallel	parallel	NN
cord-347472-n6811ens	151	18	.	.	.
cord-347472-n6811ens	152	1	Samples	sample	NNS
cord-347472-n6811ens	152	2	were	be	VBD
cord-347472-n6811ens	152	3	analyzed	analyze	VBN
cord-347472-n6811ens	152	4	using	use	VBG
cord-347472-n6811ens	152	5	the	the	DT
cord-347472-n6811ens	152	6	Viral	Viral	NNP
cord-347472-n6811ens	152	7	N1	N1	NNP
cord-347472-n6811ens	152	8	,	,	,
cord-347472-n6811ens	152	9	Viral	Viral	NNP
cord-347472-n6811ens	152	10	N2	N2	NNP
cord-347472-n6811ens	152	11	,	,	,
cord-347472-n6811ens	152	12	and	and	CC
cord-347472-n6811ens	152	13	CDC	CDC	NNP
cord-347472-n6811ens	152	14	-	-	HYPH
cord-347472-n6811ens	152	15	designed	design	VBN
cord-347472-n6811ens	152	16	RP	RP	NNP
cord-347472-n6811ens	152	17	control	control	NN
cord-347472-n6811ens	152	18	primer	primer	NN
cord-347472-n6811ens	152	19	/	/	SYM
cord-347472-n6811ens	152	20	probe	probe	NN
cord-347472-n6811ens	152	21	sets	set	NNS
cord-347472-n6811ens	152	22	,	,	,
cord-347472-n6811ens	152	23	and	and	CC
cord-347472-n6811ens	152	24	a	a	DT
cord-347472-n6811ens	152	25	transcript	transcript	NN
cord-347472-n6811ens	152	26	-	-	HYPH
cord-347472-n6811ens	152	27	specific	specific	JJ
cord-347472-n6811ens	152	28	control	control	NN
cord-347472-n6811ens	152	29	targeting	target	VBG
cord-347472-n6811ens	152	30	exons	exon	NNS
cord-347472-n6811ens	152	31	1	1	CD
cord-347472-n6811ens	152	32	-	-	SYM
cord-347472-n6811ens	152	33	3	3	CD
cord-347472-n6811ens	152	34	of	of	IN
cord-347472-n6811ens	152	35	RPP30	RPP30	NNP
cord-347472-n6811ens	153	1	(	(	-LRB-
cord-347472-n6811ens	153	2	Table	table	NN
cord-347472-n6811ens	153	3	 	 	_SP
cord-347472-n6811ens	153	4	S1	S1	NNP
cord-347472-n6811ens	153	5	)	)	-RRB-
cord-347472-n6811ens	153	6	.	.	.
cord-347472-n6811ens	154	1	Correct	correct	JJ
cord-347472-n6811ens	154	2	positive	positive	JJ
cord-347472-n6811ens	154	3	viral	viral	JJ
cord-347472-n6811ens	154	4	and	and	CC
cord-347472-n6811ens	154	5	control	control	NN
cord-347472-n6811ens	154	6	signals	signal	NNS
cord-347472-n6811ens	154	7	are	be	VBP
cord-347472-n6811ens	154	8	generated	generate	VBN
cord-347472-n6811ens	154	9	from	from	IN
cord-347472-n6811ens	154	10	each	each	DT
cord-347472-n6811ens	154	11	infected	infect	VBN
cord-347472-n6811ens	154	12	-	-	HYPH
cord-347472-n6811ens	154	13	patient	patient	NN
cord-347472-n6811ens	154	14	pool	pool	NN
cord-347472-n6811ens	154	15	after	after	IN
cord-347472-n6811ens	154	16	mock	mock	JJ
cord-347472-n6811ens	154	17	treatment	treatment	NN
cord-347472-n6811ens	154	18	(	(	-LRB-
cord-347472-n6811ens	154	19	water	water	NN
cord-347472-n6811ens	154	20	,	,	,
cord-347472-n6811ens	154	21	left	leave	VBD
cord-347472-n6811ens	154	22	)	)	-RRB-
cord-347472-n6811ens	154	23	.	.	.
cord-347472-n6811ens	155	1	Loss	loss	NN
cord-347472-n6811ens	155	2	of	of	IN
cord-347472-n6811ens	155	3	RNA	RNA	NNP
cord-347472-n6811ens	155	4	integrity	integrity	NN
cord-347472-n6811ens	155	5	causes	cause	VBZ
cord-347472-n6811ens	155	6	clinical	clinical	JJ
cord-347472-n6811ens	155	7	samples	sample	NNS
cord-347472-n6811ens	155	8	to	to	TO
cord-347472-n6811ens	155	9	return	return	VB
cord-347472-n6811ens	155	10	negative	negative	JJ
cord-347472-n6811ens	155	11	signals	signal	NNS
cord-347472-n6811ens	155	12	from	from	IN
cord-347472-n6811ens	155	13	both	both	DT
cord-347472-n6811ens	155	14	viral	viral	JJ
cord-347472-n6811ens	155	15	probes	probe	NNS
cord-347472-n6811ens	155	16	,	,	,
cord-347472-n6811ens	155	17	but	but	CC
cord-347472-n6811ens	155	18	the	the	DT
cord-347472-n6811ens	155	19	CDC	CDC	NNP
cord-347472-n6811ens	155	20	-	-	HYPH
cord-347472-n6811ens	155	21	specified	specify	VBN
cord-347472-n6811ens	155	22	RP	RP	NNP
cord-347472-n6811ens	155	23	control	control	NN
cord-347472-n6811ens	155	24	due	due	JJ
cord-347472-n6811ens	155	25	to	to	IN
cord-347472-n6811ens	155	26	DNA	dna	NN
cord-347472-n6811ens	155	27	-	-	HYPH
cord-347472-n6811ens	155	28	templated	templated	JJ
cord-347472-n6811ens	155	29	amplification	amplification	NN
cord-347472-n6811ens	155	30	(	(	-LRB-
cord-347472-n6811ens	155	31	RNase	rnase	NN
cord-347472-n6811ens	155	32	,	,	,
cord-347472-n6811ens	155	33	right	right	JJ
cord-347472-n6811ens	155	34	)	)	-RRB-
cord-347472-n6811ens	155	35	.	.	.
cord-347472-n6811ens	156	1	A	a	DT
cord-347472-n6811ens	156	2	transcript	transcript	NN
cord-347472-n6811ens	156	3	-	-	HYPH
cord-347472-n6811ens	156	4	specific	specific	JJ
cord-347472-n6811ens	156	5	RP	RP	NNP
cord-347472-n6811ens	156	6	control	control	NN
cord-347472-n6811ens	156	7	accurately	accurately	RB
cord-347472-n6811ens	156	8	informs	inform	VBZ
cord-347472-n6811ens	156	9	as	as	IN
cord-347472-n6811ens	156	10	to	to	IN
cord-347472-n6811ens	156	11	loss	loss	NN
cord-347472-n6811ens	156	12	of	of	IN
cord-347472-n6811ens	156	13	sample	sample	NN
cord-347472-n6811ens	156	14	integrity	integrity	NN
cord-347472-n6811ens	156	15	.	.	.
cord-347472-n6811ens	157	1	As	as	IN
cord-347472-n6811ens	157	2	desired	desire	VBN
cord-347472-n6811ens	157	3	,	,	,
cord-347472-n6811ens	157	4	samples	sample	NNS
cord-347472-n6811ens	157	5	with	with	IN
cord-347472-n6811ens	157	6	degraded	degrade	VBN
cord-347472-n6811ens	157	7	RNA	RNA	NNP
cord-347472-n6811ens	157	8	do	do	VBP
cord-347472-n6811ens	157	9	not	not	RB
cord-347472-n6811ens	157	10	generate	generate	VB
cord-347472-n6811ens	157	11	an	an	DT
cord-347472-n6811ens	157	12	extraction	extraction	NN
cord-347472-n6811ens	157	13	control	control	NN
cord-347472-n6811ens	157	14	signal	signal	NN
cord-347472-n6811ens	157	15	from	from	IN
cord-347472-n6811ens	157	16	a	a	DT
cord-347472-n6811ens	157	17	transcript	transcript	NN
cord-347472-n6811ens	157	18	-	-	HYPH
cord-347472-n6811ens	157	19	specific	specific	JJ
cord-347472-n6811ens	157	20	design	design	NN
cord-347472-n6811ens	157	21	,	,	,
cord-347472-n6811ens	157	22	marking	mark	VBG
cord-347472-n6811ens	157	23	the	the	DT
cord-347472-n6811ens	157	24	result	result	NN
cord-347472-n6811ens	157	25	as	as	IN
cord-347472-n6811ens	157	26	invalid	invalid	JJ
cord-347472-n6811ens	157	27	,	,	,
cord-347472-n6811ens	157	28	rather	rather	RB
cord-347472-n6811ens	157	29	than	than	IN
cord-347472-n6811ens	157	30	"	"	``
cord-347472-n6811ens	157	31	virus	virus	NN
cord-347472-n6811ens	157	32	not	not	RB
cord-347472-n6811ens	157	33	detected	detect	VBN
cord-347472-n6811ens	157	34	"	"	''
cord-347472-n6811ens	157	35	.	.	.
cord-347472-n6811ens	158	1	Horizontal	Horizontal	NNP
cord-347472-n6811ens	158	2	dashed	dash	VBD
cord-347472-n6811ens	158	3	lines	line	NNS
cord-347472-n6811ens	158	4	reflect	reflect	VBP
cord-347472-n6811ens	158	5	software	software	NN
cord-347472-n6811ens	158	6	-	-	HYPH
cord-347472-n6811ens	158	7	generated	generate	VBN
cord-347472-n6811ens	158	8	Ct	Ct	NNP
cord-347472-n6811ens	158	9	thresholds	threshold	NNS
cord-347472-n6811ens	158	10	.	.	.
cord-347472-n6811ens	159	1	Sample	sample	NN
cord-347472-n6811ens	159	2	preparation	preparation	NN
cord-347472-n6811ens	159	3	Fully	fully	RB
cord-347472-n6811ens	159	4	de	de	JJ
cord-347472-n6811ens	159	5	-	-	VBN
cord-347472-n6811ens	159	6	identified	identify	VBN
cord-347472-n6811ens	159	7	clinical	clinical	JJ
cord-347472-n6811ens	159	8	"	"	``
cord-347472-n6811ens	159	9	RNA	RNA	NNP
cord-347472-n6811ens	159	10	"	"	''
cord-347472-n6811ens	159	11	(	(	-LRB-
cord-347472-n6811ens	159	12	more	more	RBR
cord-347472-n6811ens	159	13	properly	properly	RB
cord-347472-n6811ens	159	14	,	,	,
cord-347472-n6811ens	159	15	total	total	JJ
cord-347472-n6811ens	159	16	nucleic	nucleic	JJ
cord-347472-n6811ens	159	17	acid	acid	NN
cord-347472-n6811ens	159	18	)	)	-RRB-
cord-347472-n6811ens	159	19	specimens	specimen	NNS
cord-347472-n6811ens	159	20	,	,	,
cord-347472-n6811ens	159	21	generated	generate	VBN
cord-347472-n6811ens	159	22	in	in	IN
cord-347472-n6811ens	159	23	excess	excess	NN
cord-347472-n6811ens	159	24	during	during	IN
cord-347472-n6811ens	159	25	testing	testing	NN
cord-347472-n6811ens	159	26	of	of	IN
cord-347472-n6811ens	159	27	SARS	SARS	NNP
cord-347472-n6811ens	159	28	-	-	HYPH
cord-347472-n6811ens	159	29	CoV-2	CoV-2	NNP
cord-347472-n6811ens	159	30	RT	RT	NNP
cord-347472-n6811ens	159	31	-	-	HYPH
cord-347472-n6811ens	159	32	qPCR	qpcr	JJ
cord-347472-n6811ens	159	33	-	-	HYPH
cord-347472-n6811ens	159	34	positive	positive	JJ
cord-347472-n6811ens	159	35	patients	patient	NNS
cord-347472-n6811ens	159	36	,	,	,
cord-347472-n6811ens	159	37	were	be	VBD
cord-347472-n6811ens	159	38	provided	provide	VBN
cord-347472-n6811ens	159	39	by	by	IN
cord-347472-n6811ens	159	40	the	the	DT
cord-347472-n6811ens	159	41	Stony	Stony	NNP
cord-347472-n6811ens	159	42	Brook	Brook	NNP
cord-347472-n6811ens	159	43	Hospital	Hospital	NNP
cord-347472-n6811ens	159	44	Biobank	Biobank	NNP
cord-347472-n6811ens	159	45	.	.	.
cord-347472-n6811ens	160	1	Nucleic	nucleic	JJ
cord-347472-n6811ens	160	2	acids	acid	NNS
cord-347472-n6811ens	160	3	had	have	VBD
cord-347472-n6811ens	160	4	been	be	VBN
cord-347472-n6811ens	160	5	previously	previously	RB
cord-347472-n6811ens	160	6	purified	purify	VBN
cord-347472-n6811ens	160	7	from	from	IN
cord-347472-n6811ens	160	8	polyester	polyester	NN
cord-347472-n6811ens	160	9	-	-	HYPH
cord-347472-n6811ens	160	10	tipped	tip	VBN
cord-347472-n6811ens	160	11	nasopharyngeal	nasopharyngeal	JJ
cord-347472-n6811ens	160	12	swabs	swab	NNS
cord-347472-n6811ens	160	13	desorbed	desorb	VBN
cord-347472-n6811ens	160	14	in	in	IN
cord-347472-n6811ens	160	15	viral	viral	JJ
cord-347472-n6811ens	160	16	transport	transport	NN
cord-347472-n6811ens	160	17	media	medium	NNS
cord-347472-n6811ens	160	18	(	(	-LRB-
cord-347472-n6811ens	160	19	VTM	VTM	NNP
cord-347472-n6811ens	160	20	)	)	-RRB-
cord-347472-n6811ens	160	21	and	and	CC
cord-347472-n6811ens	160	22	processed	process	VBN
cord-347472-n6811ens	160	23	in	in	IN
cord-347472-n6811ens	160	24	a	a	DT
cord-347472-n6811ens	160	25	CLIA	CLIA	NNP
cord-347472-n6811ens	160	26	high	high	JJ
cord-347472-n6811ens	160	27	complexity	complexity	NN
cord-347472-n6811ens	160	28	pathology	pathology	NN
cord-347472-n6811ens	160	29	laboratory	laboratory	NN
cord-347472-n6811ens	160	30	.	.	.
cord-347472-n6811ens	161	1	Briefly	briefly	RB
cord-347472-n6811ens	161	2	,	,	,
cord-347472-n6811ens	161	3	nucleic	nucleic	JJ
cord-347472-n6811ens	161	4	acid	acid	NN
cord-347472-n6811ens	161	5	was	be	VBD
cord-347472-n6811ens	161	6	extracted	extract	VBN
cord-347472-n6811ens	161	7	from	from	IN
cord-347472-n6811ens	161	8	160µl	160µl	JJ
cord-347472-n6811ens	161	9	VTM	VTM	NNP
cord-347472-n6811ens	161	10	using	use	VBG
cord-347472-n6811ens	161	11	Qiagen	Qiagen	NNP
cord-347472-n6811ens	161	12	QiaAMP	QiaAMP	VBZ
cord-347472-n6811ens	161	13	DSP	DSP	NNP
cord-347472-n6811ens	161	14	Viral	Viral	NNP
cord-347472-n6811ens	161	15	Mini	Mini	NNP
cord-347472-n6811ens	161	16	spin	spin	NN
cord-347472-n6811ens	161	17	columns	column	NNS
cord-347472-n6811ens	161	18	processed	process	VBN
cord-347472-n6811ens	161	19	on	on	IN
cord-347472-n6811ens	161	20	a	a	DT
cord-347472-n6811ens	161	21	Qiacube	Qiacube	NNP
cord-347472-n6811ens	161	22	automation	automation	NN
cord-347472-n6811ens	161	23	system	system	NN
cord-347472-n6811ens	161	24	as	as	IN
cord-347472-n6811ens	161	25	per	per	IN
cord-347472-n6811ens	161	26	vendor	vendor	NN
cord-347472-n6811ens	161	27	instructions	instruction	NNS
cord-347472-n6811ens	161	28	(	(	-LRB-
cord-347472-n6811ens	161	29	Qiagen	Qiagen	NNP
cord-347472-n6811ens	161	30	,	,	,
cord-347472-n6811ens	161	31	BV	BV	NNP
cord-347472-n6811ens	161	32	)	)	-RRB-
cord-347472-n6811ens	161	33	or	or	CC
cord-347472-n6811ens	161	34	from	from	IN
cord-347472-n6811ens	161	35	200µl	200µl	NNP
cord-347472-n6811ens	161	36	VTM	VTM	NNP
cord-347472-n6811ens	161	37	using	use	VBG
cord-347472-n6811ens	161	38	manual	manual	JJ
cord-347472-n6811ens	161	39	Trizol	Trizol	NNP
cord-347472-n6811ens	161	40	-	-	HYPH
cord-347472-n6811ens	161	41	mediated	mediate	VBN
cord-347472-n6811ens	161	42	organic	organic	JJ
cord-347472-n6811ens	161	43	extraction	extraction	NN
cord-347472-n6811ens	161	44	(	(	-LRB-
cord-347472-n6811ens	161	45	Life	Life	NNP
cord-347472-n6811ens	161	46	Technologies	Technologies	NNPS
cord-347472-n6811ens	161	47	)	)	-RRB-
cord-347472-n6811ens	161	48	followed	follow	VBN
cord-347472-n6811ens	161	49	by	by	IN
cord-347472-n6811ens	161	50	alcohol	alcohol	NN
cord-347472-n6811ens	161	51	precipitation	precipitation	NN
cord-347472-n6811ens	161	52	(	(	-LRB-
cord-347472-n6811ens	161	53	5	5	CD
cord-347472-n6811ens	161	54	)	)	-RRB-
cord-347472-n6811ens	161	55	.	.	.
cord-347472-n6811ens	162	1	Samples	sample	NNS
cord-347472-n6811ens	162	2	were	be	VBD
cord-347472-n6811ens	162	3	eluted	elute	VBN
cord-347472-n6811ens	162	4	in	in	IN
cord-347472-n6811ens	162	5	50µl	50µl	NN
cord-347472-n6811ens	162	6	of	of	IN
cord-347472-n6811ens	162	7	RNase	rnase	NN
cord-347472-n6811ens	162	8	-	-	HYPH
cord-347472-n6811ens	162	9	free	free	JJ
cord-347472-n6811ens	162	10	water	water	NN
cord-347472-n6811ens	162	11	supplemented	supplement	VBN
cord-347472-n6811ens	162	12	with	with	IN
cord-347472-n6811ens	162	13	0.04	0.04	CD
cord-347472-n6811ens	162	14	%	%	NN
cord-347472-n6811ens	162	15	NaN3	NaN3	NNP
cord-347472-n6811ens	162	16	(	(	-LRB-
cord-347472-n6811ens	162	17	Qiagen	Qiagen	NNP
cord-347472-n6811ens	162	18	)	)	-RRB-
cord-347472-n6811ens	162	19	or	or	CC
cord-347472-n6811ens	162	20	resuspended	resuspend	VBD
cord-347472-n6811ens	162	21	in	in	IN
cord-347472-n6811ens	162	22	RNase	rnase	NN
cord-347472-n6811ens	162	23	-	-	HYPH
cord-347472-n6811ens	162	24	free	free	JJ
cord-347472-n6811ens	162	25	water	water	NN
cord-347472-n6811ens	162	26	(	(	-LRB-
cord-347472-n6811ens	162	27	Trizol	Trizol	NNP
cord-347472-n6811ens	162	28	)	)	-RRB-
cord-347472-n6811ens	162	29	and	and	CC
cord-347472-n6811ens	162	30	stored	store	VBN
cord-347472-n6811ens	162	31	at	at	IN
cord-347472-n6811ens	162	32	-80C	-80C	NNP
cord-347472-n6811ens	162	33	until	until	IN
cord-347472-n6811ens	162	34	use	use	NN
cord-347472-n6811ens	162	35	.	.	.
cord-347472-n6811ens	163	1	Commercial	Commercial	NNP
cord-347472-n6811ens	163	2	pooled	pool	VBN
cord-347472-n6811ens	163	3	RNA	RNA	NNP
cord-347472-n6811ens	163	4	(	(	-LRB-
cord-347472-n6811ens	163	5	Quantigene	Quantigene	NNP
cord-347472-n6811ens	163	6	Human	Human	NNP
cord-347472-n6811ens	163	7	Reference	Reference	NNP
cord-347472-n6811ens	163	8	,	,	,
cord-347472-n6811ens	163	9	QS0639	qs0639	JJ
cord-347472-n6811ens	163	10	,	,	,
cord-347472-n6811ens	163	11	Life	Life	NNP
cord-347472-n6811ens	163	12	Technologies	Technologies	NNPS
cord-347472-n6811ens	163	13	)	)	-RRB-
cord-347472-n6811ens	163	14	and	and	CC
cord-347472-n6811ens	163	15	pooled	pool	VBN
cord-347472-n6811ens	163	16	human	human	JJ
cord-347472-n6811ens	163	17	male	male	JJ
cord-347472-n6811ens	163	18	DNA	dna	NN
cord-347472-n6811ens	163	19	(	(	-LRB-
cord-347472-n6811ens	163	20	G3041	G3041	NNP
cord-347472-n6811ens	163	21	,	,	,
cord-347472-n6811ens	163	22	Promega	Promega	NNP
cord-347472-n6811ens	163	23	and	and	CC
cord-347472-n6811ens	163	24	4312660	4312660	CD
cord-347472-n6811ens	163	25	,	,	,
cord-347472-n6811ens	163	26	Life	Life	NNP
cord-347472-n6811ens	163	27	Technologies	Technologies	NNPS
cord-347472-n6811ens	163	28	)	)	-RRB-
cord-347472-n6811ens	163	29	were	be	VBD
cord-347472-n6811ens	163	30	used	use	VBN
cord-347472-n6811ens	163	31	to	to	TO
cord-347472-n6811ens	163	32	generate	generate	VB
cord-347472-n6811ens	163	33	calibration	calibration	NN
cord-347472-n6811ens	163	34	curves	curve	NNS
cord-347472-n6811ens	163	35	and	and	CC
cord-347472-n6811ens	163	36	serve	serve	VBP
cord-347472-n6811ens	163	37	as	as	IN
cord-347472-n6811ens	163	38	reaction	reaction	NN
cord-347472-n6811ens	163	39	controls	control	NNS
cord-347472-n6811ens	163	40	.	.	.
cord-347472-n6811ens	164	1	DNA	dna	NN
cord-347472-n6811ens	164	2	samples	sample	NNS
cord-347472-n6811ens	164	3	were	be	VBD
cord-347472-n6811ens	164	4	diluted	dilute	VBN
cord-347472-n6811ens	164	5	in	in	IN
cord-347472-n6811ens	164	6	nuclease	nuclease	DT
cord-347472-n6811ens	164	7	free	free	JJ
cord-347472-n6811ens	164	8	water	water	NN
cord-347472-n6811ens	164	9	supplemented	supplement	VBN
cord-347472-n6811ens	164	10	with	with	IN
cord-347472-n6811ens	164	11	0.01	0.01	CD
cord-347472-n6811ens	164	12	%	%	NN
cord-347472-n6811ens	165	1	Tween-20	Tween-20	NNP
cord-347472-n6811ens	165	2	,	,	,
cord-347472-n6811ens	165	3	RNA	RNA	NNP
cord-347472-n6811ens	165	4	samples	sample	NNS
cord-347472-n6811ens	165	5	were	be	VBD
cord-347472-n6811ens	165	6	diluted	dilute	VBN
cord-347472-n6811ens	165	7	in	in	IN
cord-347472-n6811ens	165	8	nuclease	nuclease	NN
cord-347472-n6811ens	165	9	-	-	HYPH
cord-347472-n6811ens	165	10	free	free	JJ
cord-347472-n6811ens	165	11	water	water	NN
cord-347472-n6811ens	165	12	.	.	.
cord-347472-n6811ens	166	1	Low	low	JJ
cord-347472-n6811ens	166	2	binding	bind	VBG
cord-347472-n6811ens	166	3	filter	filter	NN
cord-347472-n6811ens	166	4	tips	tip	NNS
cord-347472-n6811ens	166	5	were	be	VBD
cord-347472-n6811ens	166	6	used	use	VBN
cord-347472-n6811ens	166	7	for	for	IN
cord-347472-n6811ens	166	8	all	all	DT
cord-347472-n6811ens	166	9	liquid	liquid	JJ
cord-347472-n6811ens	166	10	handling	handling	NN
cord-347472-n6811ens	166	11	.	.	.
cord-347472-n6811ens	167	1	PCR	PCR	NNP
cord-347472-n6811ens	167	2	primer	primer	NN
cord-347472-n6811ens	167	3	and	and	CC
cord-347472-n6811ens	167	4	probe	probe	NN
cord-347472-n6811ens	167	5	design	design	NN
cord-347472-n6811ens	167	6	qPCR	qpcr	NN
cord-347472-n6811ens	167	7	primers	primer	NNS
cord-347472-n6811ens	167	8	and	and	CC
cord-347472-n6811ens	167	9	hydrolysis	hydrolysis	NN
cord-347472-n6811ens	167	10	(	(	-LRB-
cord-347472-n6811ens	167	11	"	"	``
cord-347472-n6811ens	167	12	TaqMan	TaqMan	NNP
cord-347472-n6811ens	167	13	"	"	''
cord-347472-n6811ens	167	14	)	)	-RRB-
cord-347472-n6811ens	167	15	probes	probe	NNS
cord-347472-n6811ens	167	16	were	be	VBD
cord-347472-n6811ens	167	17	used	use	VBN
cord-347472-n6811ens	167	18	for	for	IN
cord-347472-n6811ens	167	19	real	real	JJ
cord-347472-n6811ens	167	20	-	-	HYPH
cord-347472-n6811ens	167	21	time	time	NN
cord-347472-n6811ens	167	22	detection	detection	NN
cord-347472-n6811ens	167	23	.	.	.
cord-347472-n6811ens	168	1	CDC	CDC	NNP
cord-347472-n6811ens	168	2	-	-	HYPH
cord-347472-n6811ens	168	3	specified	specify	VBN
cord-347472-n6811ens	168	4	RnaseP	RnaseP	NNS
cord-347472-n6811ens	168	5	(	(	-LRB-
cord-347472-n6811ens	168	6	RP	RP	NNP
cord-347472-n6811ens	168	7	)	)	-RRB-
cord-347472-n6811ens	168	8	and	and	CC
cord-347472-n6811ens	168	9	SARS	SARS	NNP
cord-347472-n6811ens	168	10	-	-	HYPH
cord-347472-n6811ens	168	11	CoV-2	CoV-2	NNP
cord-347472-n6811ens	168	12	(	(	-LRB-
cord-347472-n6811ens	168	13	N1	N1	NNP
cord-347472-n6811ens	168	14	and	and	CC
cord-347472-n6811ens	168	15	N2	N2	NNP
cord-347472-n6811ens	168	16	)	)	-RRB-
cord-347472-n6811ens	168	17	probes	probe	NNS
cord-347472-n6811ens	168	18	were	be	VBD
cord-347472-n6811ens	168	19	purchased	purchase	VBN
cord-347472-n6811ens	168	20	from	from	IN
cord-347472-n6811ens	168	21	Integrated	Integrated	NNP
cord-347472-n6811ens	168	22	DNA	DNA	NNP
cord-347472-n6811ens	168	23	Technologies	Technologies	NNP
cord-347472-n6811ens	168	24	(	(	-LRB-
cord-347472-n6811ens	168	25	RUO	RUO	NNP
cord-347472-n6811ens	168	26	qualified	qualify	VBD
cord-347472-n6811ens	168	27	,	,	,
cord-347472-n6811ens	168	28	10006713	10006713	CD
cord-347472-n6811ens	168	29	;	;	:
cord-347472-n6811ens	168	30	to	to	TO
cord-347472-n6811ens	168	31	conserve	conserve	VB
cord-347472-n6811ens	168	32	clinically	clinically	RB
cord-347472-n6811ens	168	33	certified	certify	VBN
cord-347472-n6811ens	168	34	reagents	reagent	NNS
cord-347472-n6811ens	168	35	,	,	,
cord-347472-n6811ens	168	36	research	research	NN
cord-347472-n6811ens	168	37	grade	grade	NN
cord-347472-n6811ens	168	38	primers	primer	NNS
cord-347472-n6811ens	168	39	of	of	IN
cord-347472-n6811ens	168	40	identical	identical	JJ
cord-347472-n6811ens	168	41	composition	composition	NN
cord-347472-n6811ens	168	42	were	be	VBD
cord-347472-n6811ens	168	43	used	use	VBN
cord-347472-n6811ens	168	44	)	)	-RRB-
cord-347472-n6811ens	168	45	.	.	.
cord-347472-n6811ens	169	1	CDC	CDC	NNP
cord-347472-n6811ens	169	2	primers	primer	NNS
cord-347472-n6811ens	169	3	were	be	VBD
cord-347472-n6811ens	169	4	used	use	VBN
cord-347472-n6811ens	169	5	as	as	IN
cord-347472-n6811ens	169	6	specified	specify	VBN
cord-347472-n6811ens	169	7	in	in	IN
cord-347472-n6811ens	169	8	the	the	DT
cord-347472-n6811ens	169	9	EUA	EUA	NNP
cord-347472-n6811ens	169	10	A	A	NNP
cord-347472-n6811ens	169	11	total	total	NN
cord-347472-n6811ens	169	12	of	of	IN
cord-347472-n6811ens	169	13	five	five	CD
cord-347472-n6811ens	169	14	RPP30	rpp30	IN
cord-347472-n6811ens	169	15	probe	probe	NN
cord-347472-n6811ens	169	16	set	set	VBN
cord-347472-n6811ens	169	17	designs	design	NNS
cord-347472-n6811ens	169	18	from	from	IN
cord-347472-n6811ens	169	19	multiple	multiple	JJ
cord-347472-n6811ens	169	20	vendors	vendor	NNS
cord-347472-n6811ens	169	21	were	be	VBD
cord-347472-n6811ens	169	22	ordered	order	VBN
cord-347472-n6811ens	169	23	and	and	CC
cord-347472-n6811ens	169	24	empirically	empirically	RB
cord-347472-n6811ens	169	25	tested	test	VBN
cord-347472-n6811ens	169	26	(	(	-LRB-
cord-347472-n6811ens	169	27	Table	Table	NNP
cord-347472-n6811ens	169	28	S1	S1	NNP
cord-347472-n6811ens	169	29	)	)	-RRB-
cord-347472-n6811ens	169	30	.	.	.
cord-347472-n6811ens	170	1	These	these	DT
cord-347472-n6811ens	170	2	include	include	VBP
cord-347472-n6811ens	170	3	probes	probe	NNS
cord-347472-n6811ens	170	4	targeting	target	VBG
cord-347472-n6811ens	170	5	exon	exon	NN
cord-347472-n6811ens	170	6	-	-	HYPH
cord-347472-n6811ens	170	7	exon	exon	NN
cord-347472-n6811ens	170	8	junctions	junction	NNS
cord-347472-n6811ens	170	9	and	and	CC
cord-347472-n6811ens	170	10	a	a	DT
cord-347472-n6811ens	170	11	minor	minor	JJ
cord-347472-n6811ens	170	12	-	-	HYPH
cord-347472-n6811ens	170	13	groove	groove	NN
cord-347472-n6811ens	170	14	-	-	HYPH
cord-347472-n6811ens	170	15	binding	bind	VBG
cord-347472-n6811ens	170	16	TaqMan	TaqMan	NNP
cord-347472-n6811ens	170	17	probe	probe	NN
cord-347472-n6811ens	170	18	(	(	-LRB-
cord-347472-n6811ens	170	19	Life	Life	NNP
cord-347472-n6811ens	170	20	Technologies	Technologies	NNPS
cord-347472-n6811ens	170	21	)	)	-RRB-
cord-347472-n6811ens	170	22	.	.	.
cord-347472-n6811ens	171	1	The	the	DT
cord-347472-n6811ens	171	2	best	good	JJS
cord-347472-n6811ens	171	3	performing	perform	VBG
cord-347472-n6811ens	171	4	design	design	NN
cord-347472-n6811ens	171	5	,	,	,
cord-347472-n6811ens	171	6	generating	generate	VBG
cord-347472-n6811ens	171	7	an	an	DT
cord-347472-n6811ens	171	8	amplicon	amplicon	NN
cord-347472-n6811ens	171	9	across	across	IN
cord-347472-n6811ens	171	10	exons	exon	NNS
cord-347472-n6811ens	171	11	1	1	CD
cord-347472-n6811ens	171	12	-	-	SYM
cord-347472-n6811ens	171	13	3	3	CD
cord-347472-n6811ens	171	14	with	with	IN
cord-347472-n6811ens	171	15	an	an	DT
cord-347472-n6811ens	171	16	exon	exon	NN
cord-347472-n6811ens	171	17	-	-	HYPH
cord-347472-n6811ens	171	18	exon	exon	JJ
cord-347472-n6811ens	171	19	junctionspanning	junctionspanning	NN
cord-347472-n6811ens	171	20	probe	probe	NN
cord-347472-n6811ens	171	21	,	,	,
cord-347472-n6811ens	171	22	was	be	VBD
cord-347472-n6811ens	171	23	ordered	order	VBN
cord-347472-n6811ens	171	24	with	with	IN
cord-347472-n6811ens	171	25	a	a	DT
cord-347472-n6811ens	171	26	5	5	CD
cord-347472-n6811ens	171	27	'	'	CD
cord-347472-n6811ens	171	28	6-carboxyfluoroscein	6-carboxyfluoroscein	CD
cord-347472-n6811ens	171	29	,	,	,
cord-347472-n6811ens	171	30	3-BHQ	3-bhq	CD
cord-347472-n6811ens	171	31	and	and	CC
cord-347472-n6811ens	171	32	internally	internally	RB
cord-347472-n6811ens	171	33	-	-	HYPH
cord-347472-n6811ens	171	34	quenched	quench	VBN
cord-347472-n6811ens	171	35	probe	probe	NN
cord-347472-n6811ens	171	36	from	from	IN
cord-347472-n6811ens	171	37	IDT	IDT	NNP
cord-347472-n6811ens	171	38	as	as	IN
cord-347472-n6811ens	171	39	Assay	Assay	NNP
cord-347472-n6811ens	171	40	Hs	Hs	NNP
cord-347472-n6811ens	171	41	.	.	.
cord-347472-n6811ens	172	1	PT.58.2854947	PT.58.2854947	NNP
cord-347472-n6811ens	172	2	.	.	.
cord-347472-n6811ens	173	1	This	this	DT
cord-347472-n6811ens	173	2	design	design	NN
cord-347472-n6811ens	173	3	had	have	VBD
cord-347472-n6811ens	173	4	the	the	DT
cord-347472-n6811ens	173	5	highest	high	JJS
cord-347472-n6811ens	173	6	sensitivity	sensitivity	NN
cord-347472-n6811ens	173	7	(	(	-LRB-
cord-347472-n6811ens	173	8	as	as	IN
cord-347472-n6811ens	173	9	lowest	low	JJS
cord-347472-n6811ens	173	10	Ct	Ct	NNP
cord-347472-n6811ens	173	11	)	)	-RRB-
cord-347472-n6811ens	173	12	,	,	,
cord-347472-n6811ens	173	13	highest	high	JJS
cord-347472-n6811ens	173	14	efficiency	efficiency	NN
cord-347472-n6811ens	173	15	,	,	,
cord-347472-n6811ens	173	16	and	and	CC
cord-347472-n6811ens	173	17	best	well	RBS
cord-347472-n6811ens	173	18	consistent	consistent	JJ
cord-347472-n6811ens	173	19	terminal	terminal	JJ
cord-347472-n6811ens	173	20	signal	signal	NN
cord-347472-n6811ens	173	21	characteristics	characteristic	NNS
cord-347472-n6811ens	173	22	of	of	IN
cord-347472-n6811ens	173	23	all	all	DT
cord-347472-n6811ens	173	24	candidate	candidate	NN
cord-347472-n6811ens	173	25	probes	probe	NNS
cord-347472-n6811ens	173	26	tested	test	VBD
cord-347472-n6811ens	173	27	(	(	-LRB-
cord-347472-n6811ens	173	28	Supplementary	Supplementary	NNP
cord-347472-n6811ens	173	29	Figure	Figure	NNP
cord-347472-n6811ens	173	30	S1a	S1a	NNP
cord-347472-n6811ens	173	31	)	)	-RRB-
cord-347472-n6811ens	173	32	.	.	.
cord-347472-n6811ens	174	1	All	all	DT
cord-347472-n6811ens	174	2	non	non	JJ
cord-347472-n6811ens	174	3	-	-	JJ
cord-347472-n6811ens	174	4	CDC	CDC	NNP
cord-347472-n6811ens	174	5	primer	primer	NN
cord-347472-n6811ens	174	6	/	/	SYM
cord-347472-n6811ens	174	7	probes	probe	NNS
cord-347472-n6811ens	174	8	were	be	VBD
cord-347472-n6811ens	174	9	used	use	VBN
cord-347472-n6811ens	174	10	at	at	IN
cord-347472-n6811ens	174	11	10	10	CD
cord-347472-n6811ens	174	12	pmole	pmole	NNP
cord-347472-n6811ens	174	13	primer/5	primer/5	FW
cord-347472-n6811ens	174	14	pmole	pmole	NNP
cord-347472-n6811ens	174	15	probe	probe	NN
cord-347472-n6811ens	174	16	per	per	IN
cord-347472-n6811ens	174	17	20µl	20µl	JJ
cord-347472-n6811ens	174	18	reaction	reaction	NN
cord-347472-n6811ens	174	19	.	.	.
cord-347472-n6811ens	175	1	qPCR	qPCR	NNP
cord-347472-n6811ens	175	2	reaction	reaction	NN
cord-347472-n6811ens	175	3	conditions	condition	NNS
cord-347472-n6811ens	175	4	Reverse	reverse	VBP
cord-347472-n6811ens	175	5	transcription	transcription	NN
cord-347472-n6811ens	175	6	coupled	couple	VBN
cord-347472-n6811ens	175	7	qPCR	qPCR	NNP
cord-347472-n6811ens	175	8	was	be	VBD
cord-347472-n6811ens	175	9	carried	carry	VBN
cord-347472-n6811ens	175	10	out	out	RP
cord-347472-n6811ens	175	11	essentially	essentially	RB
cord-347472-n6811ens	175	12	as	as	IN
cord-347472-n6811ens	175	13	described	describe	VBN
cord-347472-n6811ens	175	14	in	in	IN
cord-347472-n6811ens	175	15	the	the	DT
cord-347472-n6811ens	175	16	CDC	CDC	NNP
cord-347472-n6811ens	175	17	EUA	EUA	NNP
cord-347472-n6811ens	175	18	for	for	IN
cord-347472-n6811ens	175	19	COVID-19	COVID-19	NNP
cord-347472-n6811ens	175	20	testing	testing	NN
cord-347472-n6811ens	175	21	(	(	-LRB-
cord-347472-n6811ens	175	22	2	2	LS
cord-347472-n6811ens	175	23	)	)	-RRB-
cord-347472-n6811ens	175	24	with	with	IN
cord-347472-n6811ens	175	25	the	the	DT
cord-347472-n6811ens	175	26	following	following	JJ
cord-347472-n6811ens	175	27	modifications	modification	NNS
cord-347472-n6811ens	175	28	.	.	.
cord-347472-n6811ens	176	1	To	to	TO
cord-347472-n6811ens	176	2	conserve	conserve	VB
cord-347472-n6811ens	176	3	supplies	supply	NNS
cord-347472-n6811ens	176	4	for	for	IN
cord-347472-n6811ens	176	5	clinical	clinical	JJ
cord-347472-n6811ens	176	6	use	use	NN
cord-347472-n6811ens	176	7	,	,	,
cord-347472-n6811ens	176	8	comparable	comparable	JJ
cord-347472-n6811ens	176	9	research	research	NN
cord-347472-n6811ens	176	10	-	-	HYPH
cord-347472-n6811ens	176	11	grade	grade	NN
cord-347472-n6811ens	176	12	reagents	reagent	NNS
cord-347472-n6811ens	176	13	were	be	VBD
cord-347472-n6811ens	176	14	used	use	VBN
cord-347472-n6811ens	176	15	where	where	WRB
cord-347472-n6811ens	176	16	possible	possible	JJ
cord-347472-n6811ens	176	17	.	.	.
cord-347472-n6811ens	177	1	TaqMan	TaqMan	NNP
cord-347472-n6811ens	178	1	™	™	``
cord-347472-n6811ens	178	2	Fast	fast	JJ
cord-347472-n6811ens	178	3	Virus	Virus	NNP
cord-347472-n6811ens	178	4	1-Step	1-step	CD
cord-347472-n6811ens	178	5	Master	Master	NNP
cord-347472-n6811ens	178	6	Mix	Mix	NNP
cord-347472-n6811ens	178	7	(	(	-LRB-
cord-347472-n6811ens	178	8	4444432	4444432	CD
cord-347472-n6811ens	178	9	,	,	,
cord-347472-n6811ens	178	10	Life	Life	NNP
cord-347472-n6811ens	178	11	Technologies	Technologies	NNPS
cord-347472-n6811ens	178	12	)	)	-RRB-
cord-347472-n6811ens	178	13	was	be	VBD
cord-347472-n6811ens	178	14	used	use	VBN
cord-347472-n6811ens	178	15	for	for	IN
cord-347472-n6811ens	178	16	one	one	CD
cord-347472-n6811ens	178	17	-	-	HYPH
cord-347472-n6811ens	178	18	step	step	NN
cord-347472-n6811ens	178	19	RT	rt	NN
cord-347472-n6811ens	178	20	-	-	HYPH
cord-347472-n6811ens	178	21	qPCR	qPCR	NNP
cord-347472-n6811ens	178	22	and	and	CC
cord-347472-n6811ens	178	23	TaqMan	TaqMan	NNP
cord-347472-n6811ens	178	24	Fast	Fast	NNP
cord-347472-n6811ens	178	25	Advanced	Advanced	NNP
cord-347472-n6811ens	178	26	Master	Master	NNP
cord-347472-n6811ens	178	27	Mix	Mix	NNP
cord-347472-n6811ens	178	28	(	(	-LRB-
cord-347472-n6811ens	178	29	4444556	4444556	CD
cord-347472-n6811ens	178	30	,	,	,
cord-347472-n6811ens	178	31	Life	Life	NNP
cord-347472-n6811ens	178	32	Technologies	Technologies	NNPS
cord-347472-n6811ens	178	33	)	)	-RRB-
cord-347472-n6811ens	178	34	was	be	VBD
cord-347472-n6811ens	178	35	used	use	VBN
cord-347472-n6811ens	178	36	for	for	IN
cord-347472-n6811ens	178	37	non	non	JJ
cord-347472-n6811ens	178	38	-	-	JJ
cord-347472-n6811ens	178	39	reverse	reverse	JJ
cord-347472-n6811ens	178	40	transcriptase	transcriptase	NN
cord-347472-n6811ens	178	41	reactions	reaction	NNS
cord-347472-n6811ens	178	42	.	.	.
cord-347472-n6811ens	179	1	Real	real	JJ
cord-347472-n6811ens	179	2	-	-	HYPH
cord-347472-n6811ens	179	3	time	time	NN
cord-347472-n6811ens	179	4	data	datum	NNS
cord-347472-n6811ens	179	5	were	be	VBD
cord-347472-n6811ens	179	6	collected	collect	VBN
cord-347472-n6811ens	179	7	on	on	IN
cord-347472-n6811ens	179	8	a	a	DT
cord-347472-n6811ens	179	9	validated	validate	VBN
cord-347472-n6811ens	179	10	ViiA7	ViiA7	NNP
cord-347472-n6811ens	179	11	qPCR	qpcr	NN
cord-347472-n6811ens	179	12	instrument	instrument	NN
cord-347472-n6811ens	179	13	using	use	VBG
cord-347472-n6811ens	179	14	a	a	DT
cord-347472-n6811ens	179	15	384w	384w	CD
cord-347472-n6811ens	179	16	block	block	NN
cord-347472-n6811ens	179	17	and	and	CC
cord-347472-n6811ens	179	18	vendor	vendor	NN
cord-347472-n6811ens	179	19	plates	plate	NNS
cord-347472-n6811ens	179	20	and	and	CC
cord-347472-n6811ens	179	21	seals	seal	NNS
cord-347472-n6811ens	179	22	.	.	.
cord-347472-n6811ens	180	1	All	all	DT
cord-347472-n6811ens	180	2	runs	run	NNS
cord-347472-n6811ens	180	3	were	be	VBD
cord-347472-n6811ens	180	4	performed	perform	VBN
cord-347472-n6811ens	180	5	using	use	VBG
cord-347472-n6811ens	180	6	CDC	CDC	NNP
cord-347472-n6811ens	180	7	-	-	HYPH
cord-347472-n6811ens	180	8	specified	specify	VBN
cord-347472-n6811ens	180	9	thermal	thermal	JJ
cord-347472-n6811ens	180	10	cycling	cycling	NN
cord-347472-n6811ens	180	11	parameters	parameter	NNS
cord-347472-n6811ens	180	12	as	as	IN
cord-347472-n6811ens	180	13	per	per	IN
cord-347472-n6811ens	180	14	the	the	DT
cord-347472-n6811ens	180	15	EUA	EUA	NNP
cord-347472-n6811ens	180	16	.	.	.
cord-347472-n6811ens	181	1	Automatic	automatic	JJ
cord-347472-n6811ens	181	2	baselining	baselining	NN
cord-347472-n6811ens	181	3	and	and	CC
cord-347472-n6811ens	181	4	thresholding	thresholding	NN
cord-347472-n6811ens	181	5	of	of	IN
cord-347472-n6811ens	181	6	raw	raw	JJ
cord-347472-n6811ens	181	7	qPCR	qPCR	NNP
cord-347472-n6811ens	181	8	data	datum	NNS
cord-347472-n6811ens	181	9	was	be	VBD
cord-347472-n6811ens	181	10	performed	perform	VBN
cord-347472-n6811ens	181	11	using	use	VBG
cord-347472-n6811ens	181	12	vendor	vendor	NN
cord-347472-n6811ens	181	13	software	software	NN
cord-347472-n6811ens	181	14	(	(	-LRB-
cord-347472-n6811ens	181	15	QuantStudio	QuantStudio	NNP
cord-347472-n6811ens	181	16	1.3	1.3	CD
cord-347472-n6811ens	181	17	,	,	,
cord-347472-n6811ens	181	18	Life	Life	NNP
cord-347472-n6811ens	181	19	Technologies	Technologies	NNPS
cord-347472-n6811ens	181	20	)	)	-RRB-
cord-347472-n6811ens	181	21	.	.	.
cord-347472-n6811ens	182	1	Efficiency	efficiency	NN
cord-347472-n6811ens	182	2	of	of	IN
cord-347472-n6811ens	182	3	all	all	DT
cord-347472-n6811ens	182	4	qPCR	qpcr	NN
cord-347472-n6811ens	182	5	probes	probe	NNS
cord-347472-n6811ens	182	6	was	be	VBD
cord-347472-n6811ens	182	7	tested	test	VBN
cord-347472-n6811ens	182	8	by	by	IN
cord-347472-n6811ens	182	9	using	use	VBG
cord-347472-n6811ens	182	10	a	a	DT
cord-347472-n6811ens	182	11	simplex	simplex	JJ
cord-347472-n6811ens	182	12	2-fold	2-fold	CD
cord-347472-n6811ens	182	13	serial	serial	JJ
cord-347472-n6811ens	182	14	dilution	dilution	NN
cord-347472-n6811ens	182	15	of	of	IN
cord-347472-n6811ens	182	16	Quantigene	Quantigene	NNP
cord-347472-n6811ens	182	17	commercial	commercial	JJ
cord-347472-n6811ens	182	18	reference	reference	NN
cord-347472-n6811ens	182	19	human	human	NN
cord-347472-n6811ens	182	20	RNA	RNA	NNP
cord-347472-n6811ens	182	21	(	(	-LRB-
cord-347472-n6811ens	182	22	Invitrogen	Invitrogen	NNP
cord-347472-n6811ens	182	23	)	)	-RRB-
cord-347472-n6811ens	182	24	or	or	CC
cord-347472-n6811ens	182	25	DNA	DNA	NNP
cord-347472-n6811ens	182	26	(	(	-LRB-
cord-347472-n6811ens	182	27	Promega	Promega	NNP
cord-347472-n6811ens	182	28	)	)	-RRB-
cord-347472-n6811ens	182	29	ranging	range	VBG
cord-347472-n6811ens	182	30	from	from	IN
cord-347472-n6811ens	182	31	64.2ng	64.2ng	NNP
cord-347472-n6811ens	182	32	to	to	IN
cord-347472-n6811ens	182	33	62.7pg	62.7pg	CD
cord-347472-n6811ens	182	34	per	per	IN
cord-347472-n6811ens	182	35	reaction	reaction	NN
cord-347472-n6811ens	182	36	using	use	VBG
cord-347472-n6811ens	182	37	Virus	Virus	NNP
cord-347472-n6811ens	182	38	Onestep	Onestep	NNP
cord-347472-n6811ens	182	39	Mastermix	Mastermix	NNP
cord-347472-n6811ens	182	40	and	and	CC
cord-347472-n6811ens	182	41	Taqman	Taqman	NNP
cord-347472-n6811ens	182	42	Fast	Fast	NNP
cord-347472-n6811ens	182	43	Mastermix	Mastermix	NNP
cord-347472-n6811ens	182	44	.	.	.
cord-347472-n6811ens	183	1	To	to	TO
cord-347472-n6811ens	183	2	robustly	robustly	RB
cord-347472-n6811ens	183	3	determine	determine	VB
cord-347472-n6811ens	183	4	CDC	CDC	NNP
cord-347472-n6811ens	183	5	-	-	HYPH
cord-347472-n6811ens	183	6	RP	RP	NNP
cord-347472-n6811ens	183	7	response	response	NN
cord-347472-n6811ens	183	8	to	to	IN
cord-347472-n6811ens	183	9	genomic	genomic	JJ
cord-347472-n6811ens	183	10	DNA	dna	NN
cord-347472-n6811ens	183	11	,	,	,
cord-347472-n6811ens	183	12	four	four	CD
cord-347472-n6811ens	183	13	completely	completely	RB
cord-347472-n6811ens	183	14	independent	independent	JJ
cord-347472-n6811ens	183	15	dilutions	dilution	NNS
cord-347472-n6811ens	183	16	of	of	IN
cord-347472-n6811ens	183	17	genomic	genomic	JJ
cord-347472-n6811ens	183	18	DNA	dna	NN
cord-347472-n6811ens	183	19	were	be	VBD
cord-347472-n6811ens	183	20	performed	perform	VBN
cord-347472-n6811ens	183	21	down	down	RP
cord-347472-n6811ens	183	22	to	to	IN
cord-347472-n6811ens	183	23	7.8pg	7.8pg	CD
cord-347472-n6811ens	183	24	/	/	SYM
cord-347472-n6811ens	183	25	reaction	reaction	NN
cord-347472-n6811ens	183	26	and	and	CC
cord-347472-n6811ens	183	27	analyzed	analyze	VBD
cord-347472-n6811ens	183	28	using	use	VBG
cord-347472-n6811ens	183	29	Virus	Virus	NNP
cord-347472-n6811ens	183	30	One	one	CD
cord-347472-n6811ens	183	31	-	-	HYPH
cord-347472-n6811ens	183	32	Step	Step	NNP
cord-347472-n6811ens	183	33	Mastermix	Mastermix	NNP
cord-347472-n6811ens	183	34	.	.	.
cord-347472-n6811ens	184	1	Sample	sample	JJ
cord-347472-n6811ens	184	2	pooling	pooling	NN
cord-347472-n6811ens	184	3	and	and	CC
cord-347472-n6811ens	184	4	RNAse	RNAse	NNP
cord-347472-n6811ens	184	5	treatment	treatment	NN
cord-347472-n6811ens	184	6	Limited	limited	JJ
cord-347472-n6811ens	184	7	-	-	HYPH
cord-347472-n6811ens	184	8	volume	volume	NN
cord-347472-n6811ens	184	9	clinical	clinical	JJ
cord-347472-n6811ens	184	10	samples	sample	NNS
cord-347472-n6811ens	184	11	were	be	VBD
cord-347472-n6811ens	184	12	pooled	pool	VBN
cord-347472-n6811ens	184	13	to	to	TO
cord-347472-n6811ens	184	14	generate	generate	VB
cord-347472-n6811ens	184	15	sufficient	sufficient	JJ
cord-347472-n6811ens	184	16	material	material	NN
cord-347472-n6811ens	184	17	for	for	IN
cord-347472-n6811ens	184	18	the	the	DT
cord-347472-n6811ens	184	19	RNase	rnase	NN
cord-347472-n6811ens	184	20	-	-	HYPH
cord-347472-n6811ens	184	21	exposure	exposure	NN
cord-347472-n6811ens	184	22	experiment	experiment	NN
cord-347472-n6811ens	184	23	.	.	.
cord-347472-n6811ens	185	1	A	a	DT
cord-347472-n6811ens	185	2	total	total	NN
cord-347472-n6811ens	185	3	of	of	IN
cord-347472-n6811ens	185	4	28	28	CD
cord-347472-n6811ens	185	5	clinical	clinical	JJ
cord-347472-n6811ens	185	6	RNA	RNA	NNP
cord-347472-n6811ens	185	7	samples	sample	NNS
cord-347472-n6811ens	185	8	were	be	VBD
cord-347472-n6811ens	185	9	pooled	pool	VBN
cord-347472-n6811ens	185	10	,	,	,
cord-347472-n6811ens	185	11	fourteen	fourteen	CD
cord-347472-n6811ens	185	12	each	each	DT
cord-347472-n6811ens	185	13	purified	purify	VBN
cord-347472-n6811ens	185	14	by	by	IN
cord-347472-n6811ens	185	15	Qiagen	Qiagen	NNP
cord-347472-n6811ens	185	16	and	and	CC
cord-347472-n6811ens	185	17	Trizol	Trizol	NNP
cord-347472-n6811ens	185	18	(	(	-LRB-
cord-347472-n6811ens	185	19	above	above	RB
cord-347472-n6811ens	185	20	,	,	,
cord-347472-n6811ens	185	21	Sample	sample	JJ
cord-347472-n6811ens	185	22	preparation	preparation	NN
cord-347472-n6811ens	185	23	)	)	-RRB-
cord-347472-n6811ens	185	24	.	.	.
cord-347472-n6811ens	186	1	Three	three	CD
cord-347472-n6811ens	186	2	pools	pool	NNS
cord-347472-n6811ens	186	3	were	be	VBD
cord-347472-n6811ens	186	4	generated	generate	VBN
cord-347472-n6811ens	186	5	for	for	IN
cord-347472-n6811ens	186	6	each	each	DT
cord-347472-n6811ens	186	7	technology	technology	NN
cord-347472-n6811ens	186	8	,	,	,
cord-347472-n6811ens	186	9	containing	contain	VBG
cord-347472-n6811ens	186	10	5	5	CD
cord-347472-n6811ens	186	11	,	,	,
cord-347472-n6811ens	186	12	5	5	CD
cord-347472-n6811ens	186	13	,	,	,
cord-347472-n6811ens	186	14	and	and	CC
cord-347472-n6811ens	186	15	4	4	CD
cord-347472-n6811ens	186	16	clinical	clinical	JJ
cord-347472-n6811ens	186	17	samples	sample	NNS
cord-347472-n6811ens	186	18	,	,	,
cord-347472-n6811ens	186	19	respectively	respectively	RB
cord-347472-n6811ens	186	20	.	.	.
cord-347472-n6811ens	187	1	Pooling	pooling	NN
cord-347472-n6811ens	187	2	was	be	VBD
cord-347472-n6811ens	187	3	performed	perform	VBN
cord-347472-n6811ens	187	4	based	base	VBN
cord-347472-n6811ens	187	5	on	on	IN
cord-347472-n6811ens	187	6	sequence	sequence	NN
cord-347472-n6811ens	187	7	of	of	IN
cord-347472-n6811ens	187	8	the	the	DT
cord-347472-n6811ens	187	9	de	de	NN
cord-347472-n6811ens	187	10	-	-	VBN
cord-347472-n6811ens	187	11	identified	identify	VBN
cord-347472-n6811ens	187	12	serialized	serialize	VBN
cord-347472-n6811ens	187	13	sample	sample	NN
cord-347472-n6811ens	187	14	names	name	NNS
cord-347472-n6811ens	187	15	(	(	-LRB-
cord-347472-n6811ens	187	16	Q1	Q1	NNP
cord-347472-n6811ens	187	17	,	,	,
cord-347472-n6811ens	187	18	Q2	Q2	NNP
cord-347472-n6811ens	187	19	…	…	NFP
cord-347472-n6811ens	187	20	Q14	Q14	NNP
cord-347472-n6811ens	187	21	)	)	-RRB-
cord-347472-n6811ens	187	22	provided	provide	VBN
cord-347472-n6811ens	187	23	by	by	IN
cord-347472-n6811ens	187	24	the	the	DT
cord-347472-n6811ens	187	25	Stony	Stony	NNP
cord-347472-n6811ens	187	26	Brook	Brook	NNP
cord-347472-n6811ens	187	27	Hospital	Hospital	NNP
cord-347472-n6811ens	187	28	Biobank	Biobank	NNP
cord-347472-n6811ens	187	29	.	.	.
cord-347472-n6811ens	188	1	Sample	sample	JJ
cord-347472-n6811ens	188	2	names	name	NNS
cord-347472-n6811ens	188	3	do	do	VBP
cord-347472-n6811ens	188	4	not	not	RB
cord-347472-n6811ens	188	5	reflect	reflect	VB
cord-347472-n6811ens	188	6	time	time	NN
cord-347472-n6811ens	188	7	of	of	IN
cord-347472-n6811ens	188	8	collection	collection	NN
cord-347472-n6811ens	188	9	nor	nor	CC
cord-347472-n6811ens	188	10	any	any	DT
cord-347472-n6811ens	188	11	other	other	JJ
cord-347472-n6811ens	188	12	patient	patient	NN
cord-347472-n6811ens	188	13	or	or	CC
cord-347472-n6811ens	188	14	sampleintrinsic	sampleintrinsic	JJ
cord-347472-n6811ens	188	15	information	information	NN
cord-347472-n6811ens	188	16	.	.	.
cord-347472-n6811ens	189	1	Pool	pool	NN
cord-347472-n6811ens	189	2	composition	composition	NN
cord-347472-n6811ens	189	3	,	,	,
cord-347472-n6811ens	189	4	beyond	beyond	IN
cord-347472-n6811ens	189	5	positive	positive	JJ
cord-347472-n6811ens	189	6	SARS	SARS	NNP
cord-347472-n6811ens	189	7	-	-	HYPH
cord-347472-n6811ens	189	8	CoV-2	CoV-2	NNP
cord-347472-n6811ens	189	9	status	status	NN
cord-347472-n6811ens	189	10	and	and	CC
cord-347472-n6811ens	189	11	by	by	IN
cord-347472-n6811ens	189	12	extraction	extraction	NN
cord-347472-n6811ens	189	13	technology	technology	NN
cord-347472-n6811ens	189	14	,	,	,
cord-347472-n6811ens	189	15	is	be	VBZ
cord-347472-n6811ens	189	16	effectively	effectively	RB
cord-347472-n6811ens	189	17	random	random	JJ
cord-347472-n6811ens	189	18	.	.	.
cord-347472-n6811ens	190	1	8µl	8µl	NN
cord-347472-n6811ens	190	2	of	of	IN
cord-347472-n6811ens	190	3	each	each	DT
cord-347472-n6811ens	190	4	clinical	clinical	JJ
cord-347472-n6811ens	190	5	purified	purify	VBN
cord-347472-n6811ens	190	6	nucleic	nucleic	JJ
cord-347472-n6811ens	190	7	acid	acid	NN
cord-347472-n6811ens	190	8	specimen	specimen	NN
cord-347472-n6811ens	190	9	was	be	VBD
cord-347472-n6811ens	190	10	pooled	pool	VBN
cord-347472-n6811ens	190	11	into	into	IN
cord-347472-n6811ens	190	12	a	a	DT
cord-347472-n6811ens	190	13	fresh	fresh	JJ
cord-347472-n6811ens	190	14	RNase	rnase	NN
cord-347472-n6811ens	190	15	-	-	HYPH
cord-347472-n6811ens	190	16	free	free	JJ
cord-347472-n6811ens	190	17	polypropylene	polypropylene	NN
cord-347472-n6811ens	190	18	tube	tube	NN
cord-347472-n6811ens	190	19	.	.	.
cord-347472-n6811ens	191	1	8µl	8µl	NNP
cord-347472-n6811ens	191	2	of	of	IN
cord-347472-n6811ens	191	3	nuclease	nuclease	NN
cord-347472-n6811ens	191	4	-	-	HYPH
cord-347472-n6811ens	191	5	free	free	JJ
cord-347472-n6811ens	191	6	water	water	NN
cord-347472-n6811ens	191	7	was	be	VBD
cord-347472-n6811ens	191	8	added	add	VBN
cord-347472-n6811ens	191	9	to	to	IN
cord-347472-n6811ens	191	10	each	each	DT
cord-347472-n6811ens	191	11	of	of	IN
cord-347472-n6811ens	191	12	the	the	DT
cord-347472-n6811ens	191	13	four	four	CD
cord-347472-n6811ens	191	14	-	-	HYPH
cord-347472-n6811ens	191	15	specimen	specimen	NN
cord-347472-n6811ens	191	16	containing	contain	VBG
cord-347472-n6811ens	191	17	pools	pool	NNS
cord-347472-n6811ens	191	18	to	to	TO
cord-347472-n6811ens	191	19	compensate	compensate	VB
cord-347472-n6811ens	191	20	for	for	IN
cord-347472-n6811ens	191	21	the	the	DT
cord-347472-n6811ens	191	22	lower	low	JJR
cord-347472-n6811ens	191	23	specimen	specimen	NN
cord-347472-n6811ens	191	24	count	count	NN
cord-347472-n6811ens	191	25	(	(	-LRB-
cord-347472-n6811ens	191	26	resulting	result	VBG
cord-347472-n6811ens	191	27	in	in	IN
cord-347472-n6811ens	191	28	a	a	DT
cord-347472-n6811ens	191	29	total	total	JJ
cord-347472-n6811ens	191	30	volume	volume	NN
cord-347472-n6811ens	191	31	of	of	IN
cord-347472-n6811ens	191	32	40µl	40µl	NN
cord-347472-n6811ens	191	33	per	per	IN
cord-347472-n6811ens	191	34	pool	pool	NN
cord-347472-n6811ens	191	35	)	)	-RRB-
cord-347472-n6811ens	191	36	.	.	.
cord-347472-n6811ens	192	1	20µl	20µl	NN
cord-347472-n6811ens	192	2	of	of	IN
cord-347472-n6811ens	192	3	each	each	DT
cord-347472-n6811ens	192	4	pool	pool	NN
cord-347472-n6811ens	192	5	was	be	VBD
cord-347472-n6811ens	192	6	aliquoted	aliquote	VBN
cord-347472-n6811ens	192	7	to	to	IN
cord-347472-n6811ens	192	8	a	a	DT
cord-347472-n6811ens	192	9	fresh	fresh	JJ
cord-347472-n6811ens	192	10	tube	tube	NN
cord-347472-n6811ens	192	11	.	.	.
cord-347472-n6811ens	193	1	20µl	20µl	JJ
cord-347472-n6811ens	193	2	aliquots	aliquot	NNS
cord-347472-n6811ens	193	3	of	of	IN
cord-347472-n6811ens	193	4	1.73ng/µl	1.73ng/µl	CD
cord-347472-n6811ens	193	5	Promega	Promega	NNP
cord-347472-n6811ens	193	6	control	control	NN
cord-347472-n6811ens	193	7	DNA	dna	NN
cord-347472-n6811ens	193	8	and	and	CC
cord-347472-n6811ens	193	9	Quantigene	Quantigene	NNP
cord-347472-n6811ens	193	10	RNA	RNA	NNP
cord-347472-n6811ens	193	11	were	be	VBD
cord-347472-n6811ens	193	12	generated	generate	VBN
cord-347472-n6811ens	193	13	,	,	,
cord-347472-n6811ens	193	14	as	as	IN
cord-347472-n6811ens	193	15	were	be	VBD
cord-347472-n6811ens	193	16	20µl	20µl	JJ
cord-347472-n6811ens	193	17	aliquots	aliquot	NNS
cord-347472-n6811ens	193	18	of	of	IN
cord-347472-n6811ens	193	19	nuclease	nuclease	NN
cord-347472-n6811ens	193	20	-	-	HYPH
cord-347472-n6811ens	193	21	free	free	JJ
cord-347472-n6811ens	193	22	water	water	NN
cord-347472-n6811ens	193	23	as	as	IN
cord-347472-n6811ens	193	24	a	a	DT
cord-347472-n6811ens	193	25	no	no	DT
cord-347472-n6811ens	193	26	-	-	HYPH
cord-347472-n6811ens	193	27	template	template	NN
cord-347472-n6811ens	193	28	control	control	NN
cord-347472-n6811ens	193	29	.	.	.
cord-347472-n6811ens	194	1	1µl	1µl	NNP
cord-347472-n6811ens	194	2	of	of	IN
cord-347472-n6811ens	194	3	nuclease	nuclease	DT
cord-347472-n6811ens	194	4	free	free	JJ
cord-347472-n6811ens	194	5	water	water	NN
cord-347472-n6811ens	194	6	was	be	VBD
cord-347472-n6811ens	194	7	added	add	VBN
cord-347472-n6811ens	194	8	to	to	IN
cord-347472-n6811ens	194	9	each	each	DT
cord-347472-n6811ens	194	10	of	of	IN
cord-347472-n6811ens	194	11	the	the	DT
cord-347472-n6811ens	194	12	"	"	``
cord-347472-n6811ens	194	13	sham	sham	JJ
cord-347472-n6811ens	194	14	"	"	''
cord-347472-n6811ens	194	15	samples	sample	NNS
cord-347472-n6811ens	194	16	(	(	-LRB-
cord-347472-n6811ens	194	17	six	six	CD
cord-347472-n6811ens	194	18	pools	pool	NNS
cord-347472-n6811ens	194	19	plus	plus	CC
cord-347472-n6811ens	194	20	three	three	CD
cord-347472-n6811ens	194	21	controls	control	NNS
cord-347472-n6811ens	194	22	)	)	-RRB-
cord-347472-n6811ens	194	23	.	.	.
cord-347472-n6811ens	195	1	1µl	1µl	NNP
cord-347472-n6811ens	195	2	(	(	-LRB-
cord-347472-n6811ens	195	3	0.7U/µl	0.7u/µl	CD
cord-347472-n6811ens	195	4	)	)	-RRB-
cord-347472-n6811ens	195	5	of	of	IN
cord-347472-n6811ens	195	6	recombinant	recombinant	JJ
cord-347472-n6811ens	195	7	RNaseA	RNaseA	NNP
cord-347472-n6811ens	195	8	was	be	VBD
cord-347472-n6811ens	195	9	added	add	VBN
cord-347472-n6811ens	195	10	to	to	IN
cord-347472-n6811ens	195	11	each	each	DT
cord-347472-n6811ens	195	12	"	"	``
cord-347472-n6811ens	195	13	RNase	RNase	NNP
cord-347472-n6811ens	195	14	"	"	''
cord-347472-n6811ens	195	15	sample	sample	NN
cord-347472-n6811ens	195	16	in	in	IN
cord-347472-n6811ens	195	17	a	a	DT
cord-347472-n6811ens	195	18	physically	physically	RB
cord-347472-n6811ens	195	19	separate	separate	JJ
cord-347472-n6811ens	195	20	laboratory	laboratory	NN
cord-347472-n6811ens	195	21	space	space	NN
cord-347472-n6811ens	195	22	.	.	.
cord-347472-n6811ens	196	1	Capped	cap	VBN
cord-347472-n6811ens	196	2	tubes	tube	NNS
cord-347472-n6811ens	196	3	were	be	VBD
cord-347472-n6811ens	196	4	mixed	mixed	JJ
cord-347472-n6811ens	196	5	,	,	,
cord-347472-n6811ens	196	6	vortexed	vortexe	VBN
cord-347472-n6811ens	196	7	,	,	,
cord-347472-n6811ens	196	8	centrifuged	centrifuge	VBN
cord-347472-n6811ens	196	9	briefly	briefly	RB
cord-347472-n6811ens	196	10	,	,	,
cord-347472-n6811ens	196	11	and	and	CC
cord-347472-n6811ens	196	12	incubated	incubate	VBN
cord-347472-n6811ens	196	13	at	at	IN
cord-347472-n6811ens	196	14	37˚	37˚	CD
cord-347472-n6811ens	196	15	for	for	IN
cord-347472-n6811ens	196	16	90	90	CD
cord-347472-n6811ens	196	17	minutes	minute	NNS
cord-347472-n6811ens	196	18	immediately	immediately	RB
cord-347472-n6811ens	196	19	prior	prior	RB
cord-347472-n6811ens	196	20	to	to	IN
cord-347472-n6811ens	196	21	RT	RT	NNP
cord-347472-n6811ens	196	22	-	-	HYPH
cord-347472-n6811ens	196	23	qPCR	qpcr	NN
cord-347472-n6811ens	196	24	setup	setup	NN
cord-347472-n6811ens	196	25	.	.	.
cord-347472-n6811ens	197	1	One	one	CD
cord-347472-n6811ens	197	2	-	-	HYPH
cord-347472-n6811ens	197	3	step	step	NN
cord-347472-n6811ens	197	4	RT	RT	NNP
cord-347472-n6811ens	197	5	-	-	HYPH
cord-347472-n6811ens	197	6	qPCR	qPCR	NNP
cord-347472-n6811ens	197	7	was	be	VBD
cord-347472-n6811ens	197	8	performed	perform	VBN
cord-347472-n6811ens	197	9	on	on	IN
cord-347472-n6811ens	197	10	treated	treated	JJ
cord-347472-n6811ens	197	11	and	and	CC
cord-347472-n6811ens	197	12	control	control	NN
cord-347472-n6811ens	197	13	samples	sample	NNS
cord-347472-n6811ens	197	14	using	use	VBG
cord-347472-n6811ens	197	15	CDC	CDC	NNP
cord-347472-n6811ens	197	16	-	-	HYPH
cord-347472-n6811ens	197	17	RP	RP	NNP
cord-347472-n6811ens	197	18	,	,	,
cord-347472-n6811ens	197	19	CDC	CDC	NNP
cord-347472-n6811ens	197	20	-	-	HYPH
cord-347472-n6811ens	197	21	N1	N1	NNP
cord-347472-n6811ens	197	22	,	,	,
cord-347472-n6811ens	197	23	CDC	CDC	NNP
cord-347472-n6811ens	197	24	-	-	HYPH
cord-347472-n6811ens	197	25	N2	N2	NNP
cord-347472-n6811ens	197	26	,	,	,
cord-347472-n6811ens	197	27	and	and	CC
cord-347472-n6811ens	197	28	Hs	Hs	NNP
cord-347472-n6811ens	197	29	.	.	.
cord-347472-n6811ens	198	1	PT.58.2854947	PT.58.2854947	NNP
cord-347472-n6811ens	198	2	assays	assays	NNP
cord-347472-n6811ens	198	3	.	.	.
cord-347472-n6811ens	199	1	Plotting	plotting	NN
cord-347472-n6811ens	199	2	of	of	IN
cord-347472-n6811ens	199	3	raw	raw	JJ
cord-347472-n6811ens	199	4	qPCR	qpcr	NN
cord-347472-n6811ens	199	5	data	datum	NNS
cord-347472-n6811ens	199	6	Raw	Raw	NNP
cord-347472-n6811ens	199	7	qPCR	qPCR	NNP
cord-347472-n6811ens	199	8	data	datum	NNS
cord-347472-n6811ens	199	9	were	be	VBD
cord-347472-n6811ens	199	10	exported	export	VBN
cord-347472-n6811ens	199	11	as	as	IN
cord-347472-n6811ens	199	12	deltaRN	deltaRN	NNP
cord-347472-n6811ens	199	13	(	(	-LRB-
cord-347472-n6811ens	199	14	ROX	rox	NN
cord-347472-n6811ens	199	15	-	-	HYPH
cord-347472-n6811ens	199	16	normalized	normalize	VBN
cord-347472-n6811ens	199	17	,	,	,
cord-347472-n6811ens	199	18	background	background	NN
cord-347472-n6811ens	199	19	subtracted	subtract	VBN
cord-347472-n6811ens	199	20	fluorescence	fluorescence	NN
cord-347472-n6811ens	199	21	)	)	-RRB-
cord-347472-n6811ens	199	22	per	per	IN
cord-347472-n6811ens	199	23	cycle	cycle	NN
cord-347472-n6811ens	199	24	using	use	VBG
cord-347472-n6811ens	199	25	QuantStudio	QuantStudio	NNP
cord-347472-n6811ens	199	26	.	.	.
cord-347472-n6811ens	199	27	"	"	''
cord-347472-n6811ens	200	1	AmplificationData	AmplificationData	NNP
cord-347472-n6811ens	200	2	"	"	``
cord-347472-n6811ens	200	3	were	be	VBD
cord-347472-n6811ens	200	4	imported	import	VBN
cord-347472-n6811ens	200	5	into	into	IN
cord-347472-n6811ens	200	6	R	r	NN
cord-347472-n6811ens	200	7	for	for	IN
cord-347472-n6811ens	200	8	plotting	plot	VBG
cord-347472-n6811ens	200	9	.	.	.
cord-347472-n6811ens	201	1	Optical	optical	JJ
cord-347472-n6811ens	201	2	and	and	CC
cord-347472-n6811ens	201	3	quantization	quantization	NN
cord-347472-n6811ens	201	4	noise	noise	NN
cord-347472-n6811ens	201	5	result	result	NN
cord-347472-n6811ens	201	6	in	in	IN
cord-347472-n6811ens	201	7	small	small	JJ
cord-347472-n6811ens	201	8	values	value	NNS
cord-347472-n6811ens	201	9	centered	center	VBN
cord-347472-n6811ens	201	10	around	around	RB
cord-347472-n6811ens	201	11	0	0	CD
cord-347472-n6811ens	202	1	(	(	-LRB-
cord-347472-n6811ens	202	2	i.e.	i.e.	FW
cord-347472-n6811ens	202	3	either	either	CC
cord-347472-n6811ens	202	4	+	+	SYM
cord-347472-n6811ens	202	5	or	or	CC
cord-347472-n6811ens	202	6	-1x10	-1x10	NN
cord-347472-n6811ens	202	7	-4	-4	ADD
cord-347472-n6811ens	202	8	)	)	-RRB-
cord-347472-n6811ens	203	1	during	during	IN
cord-347472-n6811ens	203	2	cycles	cycle	NNS
cord-347472-n6811ens	203	3	prior	prior	RB
cord-347472-n6811ens	203	4	to	to	IN
cord-347472-n6811ens	203	5	generation	generation	NN
cord-347472-n6811ens	203	6	of	of	IN
cord-347472-n6811ens	203	7	detectable	detectable	JJ
cord-347472-n6811ens	203	8	signal	signal	NN
cord-347472-n6811ens	203	9	.	.	.
cord-347472-n6811ens	204	1	To	to	TO
cord-347472-n6811ens	204	2	eliminate	eliminate	VB
cord-347472-n6811ens	204	3	undefined	undefined	JJ
cord-347472-n6811ens	204	4	log10	log10	NN
cord-347472-n6811ens	204	5	transformation	transformation	NN
cord-347472-n6811ens	204	6	errors	error	NNS
cord-347472-n6811ens	204	7	of	of	IN
cord-347472-n6811ens	204	8	negative	negative	JJ
cord-347472-n6811ens	204	9	values	value	NNS
cord-347472-n6811ens	204	10	,	,	,
cord-347472-n6811ens	204	11	a	a	DT
cord-347472-n6811ens	204	12	small	small	JJ
cord-347472-n6811ens	204	13	offset	offset	NN
cord-347472-n6811ens	204	14	value	value	NN
cord-347472-n6811ens	204	15	of	of	IN
cord-347472-n6811ens	204	16	0.004	0.004	CD
cord-347472-n6811ens	204	17	was	be	VBD
cord-347472-n6811ens	204	18	added	add	VBN
cord-347472-n6811ens	204	19	to	to	IN
cord-347472-n6811ens	204	20	each	each	DT
cord-347472-n6811ens	204	21	DeltaRN	deltarn	RB
cord-347472-n6811ens	204	22	signal	signal	NN
cord-347472-n6811ens	204	23	prior	prior	RB
cord-347472-n6811ens	204	24	to	to	IN
cord-347472-n6811ens	204	25	plotting	plot	VBG
cord-347472-n6811ens	204	26	on	on	IN
cord-347472-n6811ens	204	27	log	log	NN
cord-347472-n6811ens	204	28	-	-	HYPH
cord-347472-n6811ens	204	29	axis	axis	NN
cord-347472-n6811ens	204	30	scale	scale	NN
cord-347472-n6811ens	204	31	.	.	.
cord-347472-n6811ens	205	1	This	this	DT
cord-347472-n6811ens	205	2	is	be	VBZ
cord-347472-n6811ens	205	3	evident	evident	JJ
cord-347472-n6811ens	205	4	as	as	IN
cord-347472-n6811ens	205	5	a	a	DT
cord-347472-n6811ens	205	6	displayed	display	VBN
cord-347472-n6811ens	205	7	baseline	baseline	JJ
cord-347472-n6811ens	205	8	signal	signal	NN
cord-347472-n6811ens	205	9	centered	center	VBN
cord-347472-n6811ens	205	10	around	around	IN
cord-347472-n6811ens	205	11	0.004	0.004	CD
cord-347472-n6811ens	205	12	in	in	IN
cord-347472-n6811ens	205	13	Figure	figure	NN
cord-347472-n6811ens	205	14	4	4	CD
cord-347472-n6811ens	205	15	.	.	.
cord-347472-n6811ens	206	1	Unmodified	unmodified	JJ
cord-347472-n6811ens	206	2	data	datum	NNS
cord-347472-n6811ens	206	3	were	be	VBD
cord-347472-n6811ens	206	4	used	use	VBN
cord-347472-n6811ens	206	5	for	for	IN
cord-347472-n6811ens	206	6	all	all	DT
cord-347472-n6811ens	206	7	calculations	calculation	NNS
cord-347472-n6811ens	206	8	and	and	CC
cord-347472-n6811ens	206	9	are	be	VBP
cord-347472-n6811ens	206	10	included	include	VBN
cord-347472-n6811ens	206	11	in	in	IN
cord-347472-n6811ens	206	12	Supplementary	Supplementary	NNP
cord-347472-n6811ens	206	13	Data	Data	NNP
cord-347472-n6811ens	206	14	;	;	:
cord-347472-n6811ens	206	15	this	this	DT
cord-347472-n6811ens	206	16	transformation	transformation	NN
cord-347472-n6811ens	206	17	is	be	VBZ
cord-347472-n6811ens	206	18	for	for	IN
cord-347472-n6811ens	206	19	convenience	convenience	NN
cord-347472-n6811ens	206	20	to	to	TO
cord-347472-n6811ens	206	21	make	make	VB
cord-347472-n6811ens	206	22	≤0	≤0	NN
cord-347472-n6811ens	206	23	data	datum	NNS
cord-347472-n6811ens	206	24	plottable	plottable	JJ
cord-347472-n6811ens	206	25	on	on	IN
cord-347472-n6811ens	206	26	a	a	DT
cord-347472-n6811ens	206	27	logarithmic	logarithmic	JJ
cord-347472-n6811ens	206	28	axis	axis	NN
cord-347472-n6811ens	206	29	.	.	.
cord-347472-n6811ens	207	1	Controls	control	NNS
cord-347472-n6811ens	207	2	are	be	VBP
cord-347472-n6811ens	207	3	useful	useful	JJ
cord-347472-n6811ens	207	4	;	;	:
cord-347472-n6811ens	207	5	why	why	WRB
cord-347472-n6811ens	207	6	not	not	RB
cord-347472-n6811ens	207	7	add	add	VB
cord-347472-n6811ens	207	8	more	more	JJR
cord-347472-n6811ens	207	9	?	?	.
cord-347472-n6811ens	208	1	Assay	assay	NN
cord-347472-n6811ens	208	2	design	design	NN
cord-347472-n6811ens	208	3	should	should	MD
cord-347472-n6811ens	208	4	be	be	VB
cord-347472-n6811ens	208	5	fit	fit	JJ
cord-347472-n6811ens	208	6	to	to	IN
cord-347472-n6811ens	208	7	task	task	NN
cord-347472-n6811ens	208	8	and	and	CC
cord-347472-n6811ens	208	9	balance	balance	NN
cord-347472-n6811ens	208	10	throughput	throughput	NN
cord-347472-n6811ens	208	11	,	,	,
cord-347472-n6811ens	208	12	cost	cost	NN
cord-347472-n6811ens	208	13	,	,	,
cord-347472-n6811ens	208	14	and	and	CC
cord-347472-n6811ens	208	15	sensitivity	sensitivity	NN
cord-347472-n6811ens	208	16	/	/	SYM
cord-347472-n6811ens	208	17	specificity	specificity	NN
cord-347472-n6811ens	208	18	.	.	.
cord-347472-n6811ens	209	1	Controls	control	NNS
cord-347472-n6811ens	209	2	are	be	VBP
cord-347472-n6811ens	209	3	necessary	necessary	JJ
cord-347472-n6811ens	209	4	,	,	,
cord-347472-n6811ens	209	5	but	but	CC
cord-347472-n6811ens	209	6	add	add	VB
cord-347472-n6811ens	209	7	assay	assay	NN
cord-347472-n6811ens	209	8	complexity	complexity	NN
cord-347472-n6811ens	209	9	,	,	,
cord-347472-n6811ens	209	10	reagent	reagent	JJ
cord-347472-n6811ens	209	11	cost	cost	NN
cord-347472-n6811ens	209	12	,	,	,
cord-347472-n6811ens	209	13	and	and	CC
cord-347472-n6811ens	209	14	can	can	MD
cord-347472-n6811ens	209	15	compete	compete	VB
cord-347472-n6811ens	209	16	with	with	IN
cord-347472-n6811ens	209	17	throughput	throughput	NN
cord-347472-n6811ens	209	18	.	.	.
cord-347472-n6811ens	210	1	The	the	DT
cord-347472-n6811ens	210	2	current	current	JJ
cord-347472-n6811ens	210	3	CDC	CDC	NNP
cord-347472-n6811ens	210	4	-	-	HYPH
cord-347472-n6811ens	210	5	EUA	EUA	NNP
cord-347472-n6811ens	210	6	uses	use	VBZ
cord-347472-n6811ens	210	7	a	a	DT
cord-347472-n6811ens	210	8	single	single	JJ
cord-347472-n6811ens	210	9	detection	detection	NN
cord-347472-n6811ens	210	10	channel	channel	NN
cord-347472-n6811ens	210	11	,	,	,
cord-347472-n6811ens	210	12	6-carboxyfluoroscein	6-carboxyfluoroscein	CD
cord-347472-n6811ens	210	13	,	,	,
cord-347472-n6811ens	210	14	for	for	IN
cord-347472-n6811ens	210	15	each	each	DT
cord-347472-n6811ens	210	16	of	of	IN
cord-347472-n6811ens	210	17	three	three	CD
cord-347472-n6811ens	210	18	probes	probe	NNS
cord-347472-n6811ens	210	19	that	that	WDT
cord-347472-n6811ens	210	20	are	be	VBP
cord-347472-n6811ens	210	21	analyzed	analyze	VBN
cord-347472-n6811ens	210	22	separately	separately	RB
cord-347472-n6811ens	210	23	.	.	.
cord-347472-n6811ens	211	1	Three	three	CD
cord-347472-n6811ens	211	2	wells	well	NNS
cord-347472-n6811ens	211	3	are	be	VBP
cord-347472-n6811ens	211	4	required	require	VBN
cord-347472-n6811ens	211	5	per	per	IN
cord-347472-n6811ens	211	6	patient	patient	NN
cord-347472-n6811ens	211	7	or	or	CC
cord-347472-n6811ens	211	8	control	control	NN
cord-347472-n6811ens	211	9	specimen	specimen	NN
cord-347472-n6811ens	211	10	.	.	.
cord-347472-n6811ens	212	1	Adding	add	VBG
cord-347472-n6811ens	212	2	even	even	RB
cord-347472-n6811ens	212	3	one	one	CD
cord-347472-n6811ens	212	4	more	more	JJR
cord-347472-n6811ens	212	5	control	control	NN
cord-347472-n6811ens	212	6	decreases	decrease	VBZ
cord-347472-n6811ens	212	7	assay	assay	NN
cord-347472-n6811ens	212	8	capacity	capacity	NN
cord-347472-n6811ens	212	9	by	by	IN
cord-347472-n6811ens	212	10	25	25	CD
cord-347472-n6811ens	212	11	%	%	NN
cord-347472-n6811ens	212	12	while	while	IN
cord-347472-n6811ens	212	13	increasing	increase	VBG
cord-347472-n6811ens	212	14	the	the	DT
cord-347472-n6811ens	212	15	cost	cost	NN
cord-347472-n6811ens	212	16	of	of	IN
cord-347472-n6811ens	212	17	One	one	CD
cord-347472-n6811ens	212	18	-	-	HYPH
cord-347472-n6811ens	212	19	step	step	NN
cord-347472-n6811ens	212	20	mastermix	mastermix	NNS
cord-347472-n6811ens	212	21	per	per	IN
cord-347472-n6811ens	212	22	patient	patient	NN
cord-347472-n6811ens	212	23	by	by	IN
cord-347472-n6811ens	212	24	the	the	DT
cord-347472-n6811ens	212	25	same	same	JJ
cord-347472-n6811ens	212	26	amount	amount	NN
cord-347472-n6811ens	212	27	.	.	.
cord-347472-n6811ens	213	1	More	More	JJR
cord-347472-n6811ens	213	2	is	be	VBZ
cord-347472-n6811ens	213	3	not	not	RB
cord-347472-n6811ens	213	4	always	always	RB
cord-347472-n6811ens	213	5	necessary	necessary	JJ
cord-347472-n6811ens	213	6	.	.	.
cord-347472-n6811ens	214	1	A	a	DT
cord-347472-n6811ens	214	2	well	well	RB
cord-347472-n6811ens	214	3	designed	design	VBN
cord-347472-n6811ens	214	4	,	,	,
cord-347472-n6811ens	214	5	transcript	transcript	NN
cord-347472-n6811ens	214	6	-	-	HYPH
cord-347472-n6811ens	214	7	specific	specific	JJ
cord-347472-n6811ens	214	8	sample	sample	NN
cord-347472-n6811ens	214	9	collection	collection	NN
cord-347472-n6811ens	214	10	,	,	,
cord-347472-n6811ens	214	11	extraction	extraction	NN
cord-347472-n6811ens	214	12	,	,	,
cord-347472-n6811ens	214	13	and	and	CC
cord-347472-n6811ens	214	14	RT	RT	NNP
cord-347472-n6811ens	214	15	-	-	HYPH
cord-347472-n6811ens	214	16	qPCR	qPCR	NNP
cord-347472-n6811ens	214	17	probe	probe	NN
cord-347472-n6811ens	214	18	can	can	MD
cord-347472-n6811ens	214	19	achieve	achieve	VB
cord-347472-n6811ens	214	20	a	a	DT
cord-347472-n6811ens	214	21	composite	composite	JJ
cord-347472-n6811ens	214	22	control	control	NN
cord-347472-n6811ens	214	23	using	use	VBG
cord-347472-n6811ens	214	24	one	one	CD
cord-347472-n6811ens	214	25	channel	channel	NN
cord-347472-n6811ens	214	26	.	.	.
cord-347472-n6811ens	215	1	If	if	IN
cord-347472-n6811ens	215	2	the	the	DT
cord-347472-n6811ens	215	3	control	control	NN
cord-347472-n6811ens	215	4	does	do	VBZ
cord-347472-n6811ens	215	5	not	not	RB
cord-347472-n6811ens	215	6	turn	turn	VB
cord-347472-n6811ens	215	7	positive	positive	JJ
cord-347472-n6811ens	215	8	,	,	,
cord-347472-n6811ens	215	9	a	a	DT
cord-347472-n6811ens	215	10	virus	virus	NN
cord-347472-n6811ens	215	11	-	-	HYPH
cord-347472-n6811ens	215	12	negative	negative	JJ
cord-347472-n6811ens	215	13	reaction	reaction	NN
cord-347472-n6811ens	215	14	can	can	MD
cord-347472-n6811ens	215	15	not	not	RB
cord-347472-n6811ens	215	16	be	be	VB
cord-347472-n6811ens	215	17	trusted	trust	VBN
cord-347472-n6811ens	215	18	.	.	.
cord-347472-n6811ens	216	1	A	a	DT
cord-347472-n6811ens	216	2	composite	composite	JJ
cord-347472-n6811ens	216	3	control	control	NN
cord-347472-n6811ens	216	4	does	do	VBZ
cord-347472-n6811ens	216	5	not	not	RB
cord-347472-n6811ens	216	6	specify	specify	VB
cord-347472-n6811ens	216	7	where	where	WRB
cord-347472-n6811ens	216	8	a	a	DT
cord-347472-n6811ens	216	9	problem	problem	NN
cord-347472-n6811ens	216	10	occurred	occur	VBD
cord-347472-n6811ens	216	11	,	,	,
cord-347472-n6811ens	216	12	only	only	RB
cord-347472-n6811ens	216	13	that	that	DT
cord-347472-n6811ens	216	14	one	one	NN
cord-347472-n6811ens	216	15	has	have	VBZ
cord-347472-n6811ens	216	16	.	.	.
cord-347472-n6811ens	217	1	Modern	modern	JJ
cord-347472-n6811ens	217	2	qPCR	qpcr	XX
cord-347472-n6811ens	218	1	(	(	-LRB-
cord-347472-n6811ens	218	2	and	and	CC
cord-347472-n6811ens	218	3	by	by	IN
cord-347472-n6811ens	218	4	extension	extension	NN
cord-347472-n6811ens	218	5	,	,	,
cord-347472-n6811ens	218	6	RT	RT	NNP
cord-347472-n6811ens	218	7	-	-	HYPH
cord-347472-n6811ens	218	8	qPCR	qPCR	NNP
cord-347472-n6811ens	218	9	)	)	-RRB-
cord-347472-n6811ens	218	10	is	be	VBZ
cord-347472-n6811ens	218	11	not	not	RB
cord-347472-n6811ens	218	12	limited	limit	VBN
cord-347472-n6811ens	218	13	to	to	IN
cord-347472-n6811ens	218	14	a	a	DT
cord-347472-n6811ens	218	15	single	single	JJ
cord-347472-n6811ens	218	16	detection	detection	NN
cord-347472-n6811ens	218	17	channel	channel	NN
cord-347472-n6811ens	218	18	;	;	,
cord-347472-n6811ens	218	19	multiplex	multiplex	JJ
cord-347472-n6811ens	218	20	designs	design	NNS
cord-347472-n6811ens	218	21	can	can	MD
cord-347472-n6811ens	218	22	generate	generate	VB
cord-347472-n6811ens	218	23	data	datum	NNS
cord-347472-n6811ens	218	24	from	from	IN
cord-347472-n6811ens	218	25	two	two	CD
cord-347472-n6811ens	218	26	to	to	TO
cord-347472-n6811ens	218	27	five	five	CD
cord-347472-n6811ens	218	28	(	(	-LRB-
cord-347472-n6811ens	218	29	or	or	CC
cord-347472-n6811ens	218	30	more	more	JJR
cord-347472-n6811ens	218	31	)	)	-RRB-
cord-347472-n6811ens	218	32	targets	target	NNS
cord-347472-n6811ens	218	33	in	in	IN
cord-347472-n6811ens	218	34	a	a	DT
cord-347472-n6811ens	218	35	single	single	JJ
cord-347472-n6811ens	218	36	well	well	NN
cord-347472-n6811ens	218	37	.	.	.
cord-347472-n6811ens	219	1	Multiple	multiple	JJ
cord-347472-n6811ens	219	2	fluorescent	fluorescent	JJ
cord-347472-n6811ens	219	3	dyes	dye	NNS
cord-347472-n6811ens	219	4	on	on	IN
cord-347472-n6811ens	219	5	probes	probe	NNS
cord-347472-n6811ens	219	6	of	of	IN
cord-347472-n6811ens	219	7	different	different	JJ
cord-347472-n6811ens	219	8	sequence	sequence	NN
cord-347472-n6811ens	219	9	can	can	MD
cord-347472-n6811ens	219	10	be	be	VB
cord-347472-n6811ens	219	11	combined	combine	VBN
cord-347472-n6811ens	219	12	in	in	IN
cord-347472-n6811ens	219	13	carefully	carefully	RB
cord-347472-n6811ens	219	14	-	-	HYPH
cord-347472-n6811ens	219	15	designed	design	VBN
cord-347472-n6811ens	219	16	assays	assay	NNS
cord-347472-n6811ens	219	17	,	,	,
cord-347472-n6811ens	219	18	increasing	increase	VBG
cord-347472-n6811ens	219	19	the	the	DT
cord-347472-n6811ens	219	20	number	number	NN
cord-347472-n6811ens	219	21	of	of	IN
cord-347472-n6811ens	219	22	targets	target	NNS
cord-347472-n6811ens	219	23	that	that	WDT
cord-347472-n6811ens	219	24	can	can	MD
cord-347472-n6811ens	219	25	be	be	VB
cord-347472-n6811ens	219	26	interrogated	interrogate	VBN
cord-347472-n6811ens	219	27	in	in	IN
cord-347472-n6811ens	219	28	one	one	CD
cord-347472-n6811ens	219	29	tube	tube	NN
cord-347472-n6811ens	219	30	.	.	.
cord-347472-n6811ens	220	1	Limitations	limitation	NNS
cord-347472-n6811ens	220	2	of	of	IN
cord-347472-n6811ens	220	3	multiplex	multiplex	JJ
cord-347472-n6811ens	220	4	qPCR	qpcr	NN
cord-347472-n6811ens	220	5	hardware	hardware	NN
cord-347472-n6811ens	220	6	,	,	,
cord-347472-n6811ens	220	7	fluorescent	fluorescent	NN
cord-347472-n6811ens	220	8	dyes	dye	NNS
cord-347472-n6811ens	220	9	,	,	,
cord-347472-n6811ens	220	10	and	and	CC
cord-347472-n6811ens	220	11	the	the	DT
cord-347472-n6811ens	220	12	realities	reality	NNS
cord-347472-n6811ens	220	13	of	of	IN
cord-347472-n6811ens	220	14	molecular	molecular	JJ
cord-347472-n6811ens	220	15	biology	biology	NN
cord-347472-n6811ens	220	16	constrain	constrain	VBP
cord-347472-n6811ens	220	17	the	the	DT
cord-347472-n6811ens	220	18	degree	degree	NN
cord-347472-n6811ens	220	19	of	of	IN
cord-347472-n6811ens	220	20	multiplexing	multiplexing	NN
cord-347472-n6811ens	220	21	and	and	CC
cord-347472-n6811ens	220	22	drive	drive	VB
cord-347472-n6811ens	220	23	design	design	NN
cord-347472-n6811ens	220	24	concessions	concession	NNS
cord-347472-n6811ens	220	25	.	.	.
cord-347472-n6811ens	221	1	Multiplex	multiplex	JJ
cord-347472-n6811ens	221	2	designs	design	NNS
cord-347472-n6811ens	221	3	are	be	VBP
cord-347472-n6811ens	221	4	widely	widely	RB
cord-347472-n6811ens	221	5	used	use	VBN
cord-347472-n6811ens	221	6	in	in	IN
cord-347472-n6811ens	221	7	the	the	DT
cord-347472-n6811ens	221	8	clinic	clinic	NN
cord-347472-n6811ens	221	9	,	,	,
cord-347472-n6811ens	221	10	including	include	VBG
cord-347472-n6811ens	221	11	for	for	IN
cord-347472-n6811ens	221	12	SARS	SARS	NNP
cord-347472-n6811ens	221	13	-	-	HYPH
cord-347472-n6811ens	221	14	CoV-2	CoV-2	NNP
cord-347472-n6811ens	221	15	testing	testing	NN
cord-347472-n6811ens	221	16	.	.	.
cord-347472-n6811ens	222	1	The	the	DT
cord-347472-n6811ens	222	2	ability	ability	NN
cord-347472-n6811ens	222	3	to	to	TO
cord-347472-n6811ens	222	4	multiplex	multiplex	NN
cord-347472-n6811ens	222	5	does	do	VBZ
cord-347472-n6811ens	222	6	not	not	RB
cord-347472-n6811ens	222	7	obviate	obviate	VB
cord-347472-n6811ens	222	8	good	good	JJ
cord-347472-n6811ens	222	9	assay	assay	NN
cord-347472-n6811ens	222	10	design	design	NN
cord-347472-n6811ens	222	11	.	.	.
cord-347472-n6811ens	223	1	Several	several	JJ
cord-347472-n6811ens	223	2	current	current	JJ
cord-347472-n6811ens	223	3	panel	panel	NN
cord-347472-n6811ens	223	4	designs	design	NNS
cord-347472-n6811ens	223	5	use	use	VBP
cord-347472-n6811ens	223	6	a	a	DT
cord-347472-n6811ens	223	7	synthetic	synthetic	JJ
cord-347472-n6811ens	223	8	RNA	rna	NN
cord-347472-n6811ens	223	9	spike	spike	NN
cord-347472-n6811ens	223	10	-	-	HYPH
cord-347472-n6811ens	223	11	in	in	NN
cord-347472-n6811ens	223	12	to	to	TO
cord-347472-n6811ens	223	13	monitor	monitor	VB
cord-347472-n6811ens	223	14	extraction	extraction	NN
cord-347472-n6811ens	223	15	and	and	CC
cord-347472-n6811ens	223	16	reverse	reverse	VB
cord-347472-n6811ens	223	17	transcription	transcription	NN
cord-347472-n6811ens	223	18	/	/	SYM
cord-347472-n6811ens	223	19	PCR	pcr	NN
cord-347472-n6811ens	223	20	.	.	.
cord-347472-n6811ens	224	1	Patient	patient	JJ
cord-347472-n6811ens	224	2	sampling	sampling	NN
cord-347472-n6811ens	224	3	and	and	CC
cord-347472-n6811ens	224	4	extraction	extraction	NN
cord-347472-n6811ens	224	5	controls	control	NNS
cord-347472-n6811ens	224	6	are	be	VBP
cord-347472-n6811ens	224	7	critical	critical	JJ
cord-347472-n6811ens	224	8	to	to	IN
cord-347472-n6811ens	224	9	clinical	clinical	JJ
cord-347472-n6811ens	224	10	interpretation	interpretation	NN
cord-347472-n6811ens	224	11	and	and	CC
cord-347472-n6811ens	224	12	should	should	MD
cord-347472-n6811ens	224	13	be	be	VB
cord-347472-n6811ens	224	14	prioritized	prioritize	VBN
cord-347472-n6811ens	224	15	.	.	.
cord-347472-n6811ens	225	1	As	as	IN
cord-347472-n6811ens	225	2	of	of	IN
cord-347472-n6811ens	225	3	May	May	NNP
cord-347472-n6811ens	225	4	12	12	CD
cord-347472-n6811ens	225	5	,	,	,
cord-347472-n6811ens	225	6	2020	2020	CD
cord-347472-n6811ens	225	7	,	,	,
cord-347472-n6811ens	225	8	none	none	NN
cord-347472-n6811ens	225	9	of	of	IN
cord-347472-n6811ens	225	10	the	the	DT
cord-347472-n6811ens	225	11	FDA	FDA	NNP
cord-347472-n6811ens	225	12	EUA'd	EUA'd	NNP
cord-347472-n6811ens	225	13	SARS	SARS	NNP
cord-347472-n6811ens	225	14	-	-	HYPH
cord-347472-n6811ens	225	15	CoV-2	CoV-2	NNP
cord-347472-n6811ens	225	16	assay	assay	NN
cord-347472-n6811ens	225	17	designs	design	NNS
cord-347472-n6811ens	225	18	contain	contain	VBP
cord-347472-n6811ens	225	19	both	both	CC
cord-347472-n6811ens	225	20	a	a	DT
cord-347472-n6811ens	225	21	human	human	JJ
cord-347472-n6811ens	225	22	sample	sample	NN
cord-347472-n6811ens	225	23	collection	collection	NN
cord-347472-n6811ens	225	24	control	control	NN
cord-347472-n6811ens	225	25	and	and	CC
cord-347472-n6811ens	225	26	a	a	DT
cord-347472-n6811ens	225	27	separate	separate	JJ
cord-347472-n6811ens	225	28	extraction	extraction	NN
cord-347472-n6811ens	225	29	/	/	SYM
cord-347472-n6811ens	225	30	RT	RT	NNP
cord-347472-n6811ens	225	31	control	control	NN
cord-347472-n6811ens	225	32	.	.	.
cord-347472-n6811ens	226	1	Four	four	CD
cord-347472-n6811ens	226	2	-	-	HYPH
cord-347472-n6811ens	226	3	plex	plex	NNP
cord-347472-n6811ens	226	4	RT	RT	NNP
cord-347472-n6811ens	226	5	-	-	HYPH
cord-347472-n6811ens	226	6	qPCR	qPCR	NNP
cord-347472-n6811ens	226	7	,	,	,
cord-347472-n6811ens	226	8	used	use	VBN
cord-347472-n6811ens	226	9	by	by	IN
cord-347472-n6811ens	226	10	several	several	JJ
cord-347472-n6811ens	226	11	panels	panel	NNS
cord-347472-n6811ens	226	12	,	,	,
cord-347472-n6811ens	226	13	could	could	MD
cord-347472-n6811ens	226	14	be	be	VB
cord-347472-n6811ens	226	15	leveraged	leverage	VBN
cord-347472-n6811ens	226	16	as	as	IN
cord-347472-n6811ens	226	17	a	a	DT
cord-347472-n6811ens	226	18	pair	pair	NN
cord-347472-n6811ens	226	19	of	of	IN
cord-347472-n6811ens	226	20	viral	viral	JJ
cord-347472-n6811ens	226	21	probes	probe	NNS
cord-347472-n6811ens	226	22	,	,	,
cord-347472-n6811ens	226	23	an	an	DT
cord-347472-n6811ens	226	24	RNA	RNA	NNP
cord-347472-n6811ens	226	25	spike	spike	NN
cord-347472-n6811ens	226	26	to	to	TO
cord-347472-n6811ens	226	27	monitor	monitor	VB
cord-347472-n6811ens	226	28	extraction	extraction	NN
cord-347472-n6811ens	226	29	/	/	SYM
cord-347472-n6811ens	226	30	RT	RT	NNP
cord-347472-n6811ens	226	31	-	-	HYPH
cord-347472-n6811ens	226	32	qPCR	qPCR	NNP
cord-347472-n6811ens	226	33	,	,	,
cord-347472-n6811ens	226	34	and	and	CC
cord-347472-n6811ens	226	35	an	an	DT
cord-347472-n6811ens	226	36	RNA	rna	NN
cord-347472-n6811ens	226	37	-	-	HYPH
cord-347472-n6811ens	226	38	specific	specific	JJ
cord-347472-n6811ens	226	39	human	human	JJ
cord-347472-n6811ens	226	40	specimen	specimen	NN
cord-347472-n6811ens	226	41	control	control	NN
cord-347472-n6811ens	226	42	.	.	.
cord-347472-n6811ens	227	1	This	this	DT
cord-347472-n6811ens	227	2	approach	approach	NN
cord-347472-n6811ens	227	3	retains	retain	VBZ
cord-347472-n6811ens	227	4	control	control	NN
cord-347472-n6811ens	227	5	for	for	IN
cord-347472-n6811ens	227	6	presence	presence	NN
cord-347472-n6811ens	227	7	of	of	IN
cord-347472-n6811ens	227	8	a	a	DT
cord-347472-n6811ens	227	9	human	human	JJ
cord-347472-n6811ens	227	10	specimen	specimen	NN
cord-347472-n6811ens	227	11	while	while	IN
cord-347472-n6811ens	227	12	providing	provide	VBG
cord-347472-n6811ens	227	13	more	more	JJR
cord-347472-n6811ens	227	14	granularity	granularity	NN
cord-347472-n6811ens	227	15	than	than	IN
cord-347472-n6811ens	227	16	available	available	JJ
cord-347472-n6811ens	227	17	from	from	IN
cord-347472-n6811ens	227	18	a	a	DT
cord-347472-n6811ens	227	19	single	single	JJ
cord-347472-n6811ens	227	20	composite	composite	JJ
cord-347472-n6811ens	227	21	control	control	NN
cord-347472-n6811ens	227	22	,	,	,
cord-347472-n6811ens	227	23	such	such	JJ
cord-347472-n6811ens	227	24	as	as	IN
cord-347472-n6811ens	227	25	a	a	DT
cord-347472-n6811ens	227	26	transcript	transcript	NN
cord-347472-n6811ens	227	27	-	-	HYPH
cord-347472-n6811ens	227	28	specific	specific	JJ
cord-347472-n6811ens	227	29	RPP30	RPP30	NNP
cord-347472-n6811ens	227	30	probe	probe	NN
cord-347472-n6811ens	227	31	.	.	.
cord-347472-n6811ens	228	1	This	this	DT
cord-347472-n6811ens	228	2	two	two	CD
cord-347472-n6811ens	228	3	-	-	HYPH
cord-347472-n6811ens	228	4	control	control	NN
cord-347472-n6811ens	228	5	design	design	NN
cord-347472-n6811ens	228	6	would	would	MD
cord-347472-n6811ens	228	7	enable	enable	VB
cord-347472-n6811ens	228	8	more	more	RBR
cord-347472-n6811ens	228	9	efficient	efficient	JJ
cord-347472-n6811ens	228	10	handling	handling	NN
cord-347472-n6811ens	228	11	of	of	IN
cord-347472-n6811ens	228	12	specimens	specimen	NNS
cord-347472-n6811ens	228	13	where	where	WRB
cord-347472-n6811ens	228	14	no	no	DT
cord-347472-n6811ens	228	15	human	human	JJ
cord-347472-n6811ens	228	16	specimen	specimen	NN
cord-347472-n6811ens	228	17	control	control	NN
cord-347472-n6811ens	228	18	signal	signal	NN
cord-347472-n6811ens	228	19	is	be	VBZ
cord-347472-n6811ens	228	20	generated	generate	VBN
cord-347472-n6811ens	228	21	.	.	.
cord-347472-n6811ens	229	1	An	an	DT
cord-347472-n6811ens	229	2	intact	intact	JJ
cord-347472-n6811ens	229	3	spike	spike	NN
cord-347472-n6811ens	229	4	-	-	HYPH
cord-347472-n6811ens	229	5	in	in	RP
cord-347472-n6811ens	229	6	signal	signal	NN
cord-347472-n6811ens	229	7	would	would	MD
cord-347472-n6811ens	229	8	mean	mean	VB
cord-347472-n6811ens	229	9	that	that	IN
cord-347472-n6811ens	229	10	that	that	DT
cord-347472-n6811ens	229	11	extraction	extraction	NN
cord-347472-n6811ens	229	12	and	and	CC
cord-347472-n6811ens	229	13	downstream	downstream	JJ
cord-347472-n6811ens	229	14	handling	handling	NN
cord-347472-n6811ens	229	15	of	of	IN
cord-347472-n6811ens	229	16	RNA	RNA	NNP
cord-347472-n6811ens	229	17	through	through	IN
cord-347472-n6811ens	229	18	RT	RT	NNP
cord-347472-n6811ens	229	19	-	-	HYPH
cord-347472-n6811ens	229	20	qPCR	qPCR	NNP
cord-347472-n6811ens	229	21	were	be	VBD
cord-347472-n6811ens	229	22	successful	successful	JJ
cord-347472-n6811ens	229	23	,	,	,
cord-347472-n6811ens	229	24	despite	despite	IN
cord-347472-n6811ens	229	25	no	no	DT
cord-347472-n6811ens	229	26	patient	patient	NN
cord-347472-n6811ens	229	27	RNA	RNA	NNP
cord-347472-n6811ens	229	28	having	have	VBG
cord-347472-n6811ens	229	29	been	be	VBN
cord-347472-n6811ens	229	30	obtained	obtain	VBN
cord-347472-n6811ens	229	31	.	.	.
cord-347472-n6811ens	230	1	Patients	patient	NNS
cord-347472-n6811ens	230	2	should	should	MD
cord-347472-n6811ens	230	3	be	be	VB
cord-347472-n6811ens	230	4	called	call	VBN
cord-347472-n6811ens	230	5	back	back	RB
cord-347472-n6811ens	230	6	in	in	RB
cord-347472-n6811ens	230	7	,	,	,
cord-347472-n6811ens	230	8	re	re	VBN
cord-347472-n6811ens	230	9	-	-	VBN
cord-347472-n6811ens	230	10	sampled	sample	VBN
cord-347472-n6811ens	230	11	,	,	,
cord-347472-n6811ens	230	12	re	re	VBN
cord-347472-n6811ens	230	13	-	-	VBN
cord-347472-n6811ens	230	14	extracted	extract	VBN
cord-347472-n6811ens	230	15	,	,	,
cord-347472-n6811ens	230	16	and	and	CC
cord-347472-n6811ens	230	17	re	re	VBN
cord-347472-n6811ens	230	18	-	-	VBN
cord-347472-n6811ens	230	19	tested	test	VBN
cord-347472-n6811ens	230	20	.	.	.
cord-347472-n6811ens	231	1	In	in	IN
cord-347472-n6811ens	231	2	contrast	contrast	NN
cord-347472-n6811ens	231	3	,	,	,
cord-347472-n6811ens	231	4	failure	failure	NN
cord-347472-n6811ens	231	5	of	of	IN
cord-347472-n6811ens	231	6	the	the	DT
cord-347472-n6811ens	231	7	spike	spike	NN
cord-347472-n6811ens	231	8	-	-	HYPH
cord-347472-n6811ens	231	9	in	in	RP
cord-347472-n6811ens	231	10	control	control	NN
cord-347472-n6811ens	231	11	means	mean	VBZ
cord-347472-n6811ens	231	12	that	that	IN
cord-347472-n6811ens	231	13	something	something	NN
cord-347472-n6811ens	231	14	likely	likely	RB
cord-347472-n6811ens	231	15	went	go	VBD
cord-347472-n6811ens	231	16	amiss	amiss	JJ
cord-347472-n6811ens	231	17	during	during	IN
cord-347472-n6811ens	231	18	handling	handling	NN
cord-347472-n6811ens	231	19	.	.	.
cord-347472-n6811ens	232	1	Whether	whether	IN
cord-347472-n6811ens	232	2	caused	cause	VBN
cord-347472-n6811ens	232	3	by	by	IN
cord-347472-n6811ens	232	4	a	a	DT
cord-347472-n6811ens	232	5	bad	bad	JJ
cord-347472-n6811ens	232	6	pipet	pipet	NN
cord-347472-n6811ens	232	7	tip	tip	NN
cord-347472-n6811ens	232	8	,	,	,
cord-347472-n6811ens	232	9	a	a	DT
cord-347472-n6811ens	232	10	one	one	CD
cord-347472-n6811ens	232	11	-	-	HYPH
cord-347472-n6811ens	232	12	off	off	NN
cord-347472-n6811ens	232	13	problem	problem	NN
cord-347472-n6811ens	232	14	with	with	IN
cord-347472-n6811ens	232	15	extraction	extraction	NN
cord-347472-n6811ens	232	16	,	,	,
cord-347472-n6811ens	232	17	or	or	CC
cord-347472-n6811ens	232	18	shaky	shaky	JJ
cord-347472-n6811ens	232	19	hands	hand	NNS
cord-347472-n6811ens	232	20	at	at	IN
cord-347472-n6811ens	232	21	the	the	DT
cord-347472-n6811ens	232	22	end	end	NN
cord-347472-n6811ens	232	23	of	of	IN
cord-347472-n6811ens	232	24	a	a	DT
cord-347472-n6811ens	232	25	shift	shift	NN
cord-347472-n6811ens	232	26	,	,	,
cord-347472-n6811ens	232	27	excess	excess	JJ
cord-347472-n6811ens	232	28	viral	viral	JJ
cord-347472-n6811ens	232	29	transport	transport	NN
cord-347472-n6811ens	232	30	media	medium	NNS
cord-347472-n6811ens	232	31	could	could	MD
cord-347472-n6811ens	232	32	be	be	VB
cord-347472-n6811ens	232	33	re	re	VBN
cord-347472-n6811ens	232	34	-	-	VBN
cord-347472-n6811ens	232	35	extracted	extract	VBN
cord-347472-n6811ens	233	1	and	and	CC
cord-347472-n6811ens	233	2	re	re	NNS
cord-347472-n6811ens	233	3	-	-	VBN
cord-347472-n6811ens	233	4	tested	test	VBN
cord-347472-n6811ens	233	5	before	before	IN
cord-347472-n6811ens	233	6	recalling	recall	VBG
cord-347472-n6811ens	233	7	a	a	DT
cord-347472-n6811ens	233	8	patient	patient	NN
cord-347472-n6811ens	233	9	into	into	IN
cord-347472-n6811ens	233	10	the	the	DT
cord-347472-n6811ens	233	11	health	health	NN
cord-347472-n6811ens	233	12	care	care	NN
cord-347472-n6811ens	233	13	setting	set	VBG
cord-347472-n6811ens	233	14	for	for	IN
cord-347472-n6811ens	233	15	a	a	DT
cord-347472-n6811ens	233	16	re	re	NN
cord-347472-n6811ens	233	17	-	-	NN
cord-347472-n6811ens	233	18	swabbing	swabbing	NN
cord-347472-n6811ens	233	19	.	.	.
cord-347472-n6811ens	234	1	Why	why	WRB
cord-347472-n6811ens	234	2	change	change	VB
cord-347472-n6811ens	234	3	the	the	DT
cord-347472-n6811ens	234	4	assay	assay	NN
cord-347472-n6811ens	234	5	when	when	WRB
cord-347472-n6811ens	234	6	one	one	PRP
cord-347472-n6811ens	234	7	could	could	MD
cord-347472-n6811ens	234	8	fix	fix	VB
cord-347472-n6811ens	234	9	the	the	DT
cord-347472-n6811ens	234	10	input	input	NN
cord-347472-n6811ens	234	11	?	?	.
cord-347472-n6811ens	235	1	Although	although	IN
cord-347472-n6811ens	235	2	a	a	DT
cord-347472-n6811ens	235	3	re	re	NN
cord-347472-n6811ens	235	4	-	-	JJ
cord-347472-n6811ens	235	5	designed	design	VBN
cord-347472-n6811ens	235	6	transcript	transcript	NN
cord-347472-n6811ens	235	7	-	-	HYPH
cord-347472-n6811ens	235	8	specific	specific	JJ
cord-347472-n6811ens	235	9	control	control	NN
cord-347472-n6811ens	235	10	eliminates	eliminate	VBZ
cord-347472-n6811ens	235	11	the	the	DT
cord-347472-n6811ens	235	12	false	false	JJ
cord-347472-n6811ens	235	13	negative	negative	JJ
cord-347472-n6811ens	235	14	potential	potential	NN
cord-347472-n6811ens	235	15	described	describe	VBN
cord-347472-n6811ens	235	16	here	here	RB
cord-347472-n6811ens	235	17	,	,	,
cord-347472-n6811ens	235	18	approaches	approach	VBZ
cord-347472-n6811ens	235	19	to	to	TO
cord-347472-n6811ens	235	20	make	make	VB
cord-347472-n6811ens	235	21	best	good	JJS
cord-347472-n6811ens	235	22	use	use	NN
cord-347472-n6811ens	235	23	of	of	IN
cord-347472-n6811ens	235	24	the	the	DT
cord-347472-n6811ens	235	25	current	current	JJ
cord-347472-n6811ens	235	26	reagent	reagent	NN
cord-347472-n6811ens	235	27	will	will	MD
cord-347472-n6811ens	235	28	likely	likely	RB
cord-347472-n6811ens	235	29	be	be	VB
cord-347472-n6811ens	235	30	proposed	propose	VBN
cord-347472-n6811ens	235	31	.	.	.
cord-347472-n6811ens	236	1	One	one	CD
cord-347472-n6811ens	236	2	potential	potential	JJ
cord-347472-n6811ens	236	3	alternative	alternative	NN
cord-347472-n6811ens	236	4	to	to	TO
cord-347472-n6811ens	236	5	eliminate	eliminate	VB
cord-347472-n6811ens	236	6	confounding	confounding	JJ
cord-347472-n6811ens	236	7	genomic	genomic	JJ
cord-347472-n6811ens	236	8	DNA	dna	NN
cord-347472-n6811ens	236	9	is	be	VBZ
cord-347472-n6811ens	236	10	to	to	TO
cord-347472-n6811ens	236	11	digest	digest	VB
cord-347472-n6811ens	236	12	extracted	extract	VBN
cord-347472-n6811ens	236	13	samples	sample	NNS
cord-347472-n6811ens	236	14	with	with	IN
cord-347472-n6811ens	236	15	deoxyribonuclease	deoxyribonuclease	NN
cord-347472-n6811ens	236	16	(	(	-LRB-
cord-347472-n6811ens	236	17	DNase	DNase	NNP
cord-347472-n6811ens	236	18	)	)	-RRB-
cord-347472-n6811ens	236	19	.	.	.
cord-347472-n6811ens	237	1	This	this	DT
cord-347472-n6811ens	237	2	should	should	MD
cord-347472-n6811ens	237	3	be	be	VB
cord-347472-n6811ens	237	4	avoided	avoid	VBN
cord-347472-n6811ens	237	5	.	.	.
cord-347472-n6811ens	238	1	This	this	DT
cord-347472-n6811ens	238	2	approach	approach	NN
cord-347472-n6811ens	238	3	is	be	VBZ
cord-347472-n6811ens	238	4	expensive	expensive	JJ
cord-347472-n6811ens	238	5	(	(	-LRB-
cord-347472-n6811ens	238	6	far	far	RB
cord-347472-n6811ens	238	7	more	more	JJR
cord-347472-n6811ens	238	8	than	than	IN
cord-347472-n6811ens	238	9	a	a	DT
cord-347472-n6811ens	238	10	re	re	NN
cord-347472-n6811ens	238	11	-	-	JJ
cord-347472-n6811ens	238	12	designed	design	VBN
cord-347472-n6811ens	238	13	primer	primer	NN
cord-347472-n6811ens	238	14	)	)	-RRB-
cord-347472-n6811ens	238	15	,	,	,
cord-347472-n6811ens	238	16	requires	require	VBZ
cord-347472-n6811ens	238	17	addition	addition	NN
cord-347472-n6811ens	238	18	of	of	IN
cord-347472-n6811ens	238	19	divalent	divalent	JJ
cord-347472-n6811ens	238	20	cations	cation	NNS
cord-347472-n6811ens	238	21	that	that	WDT
cord-347472-n6811ens	238	22	can	can	MD
cord-347472-n6811ens	238	23	interfere	interfere	VB
cord-347472-n6811ens	238	24	with	with	IN
cord-347472-n6811ens	238	25	downstream	downstream	JJ
cord-347472-n6811ens	238	26	reverse	reverse	NN
cord-347472-n6811ens	238	27	transcription	transcription	NN
cord-347472-n6811ens	238	28	and	and	CC
cord-347472-n6811ens	238	29	PCR	PCR	NNP
cord-347472-n6811ens	238	30	,	,	,
cord-347472-n6811ens	238	31	and	and	CC
cord-347472-n6811ens	238	32	is	be	VBZ
cord-347472-n6811ens	238	33	imperfect	imperfect	JJ
cord-347472-n6811ens	238	34	:	:	:
cord-347472-n6811ens	238	35	DNA	DNA	NNP
cord-347472-n6811ens	238	36	has	have	VBZ
cord-347472-n6811ens	238	37	evolved	evolve	VBN
cord-347472-n6811ens	238	38	to	to	TO
cord-347472-n6811ens	238	39	be	be	VB
cord-347472-n6811ens	238	40	more	more	RBR
cord-347472-n6811ens	238	41	physically	physically	RB
cord-347472-n6811ens	238	42	robust	robust	JJ
cord-347472-n6811ens	238	43	than	than	IN
cord-347472-n6811ens	238	44	RNA	RNA	NNP
cord-347472-n6811ens	238	45	.	.	.
cord-347472-n6811ens	239	1	As	as	IN
cord-347472-n6811ens	239	2	shown	show	VBN
cord-347472-n6811ens	239	3	above	above	RB
cord-347472-n6811ens	239	4	and	and	CC
cord-347472-n6811ens	239	5	in	in	IN
cord-347472-n6811ens	239	6	many	many	JJ
cord-347472-n6811ens	239	7	other	other	JJ
cord-347472-n6811ens	239	8	contexts	contexts	NN
cord-347472-n6811ens	239	9	,	,	,
cord-347472-n6811ens	239	10	exponential	exponential	JJ
cord-347472-n6811ens	239	11	amplification	amplification	NN
cord-347472-n6811ens	239	12	during	during	IN
cord-347472-n6811ens	239	13	PCR	PCR	NNP
cord-347472-n6811ens	239	14	generates	generate	VBZ
cord-347472-n6811ens	239	15	detectable	detectable	JJ
cord-347472-n6811ens	239	16	signals	signal	NNS
cord-347472-n6811ens	239	17	from	from	IN
cord-347472-n6811ens	239	18	single	single	JJ
cord-347472-n6811ens	239	19	-	-	HYPH
cord-347472-n6811ens	239	20	digit	digit	NN
cord-347472-n6811ens	239	21	copies	copy	NNS
cord-347472-n6811ens	239	22	of	of	IN
cord-347472-n6811ens	239	23	template	template	NN
cord-347472-n6811ens	239	24	.	.	.
cord-347472-n6811ens	240	1	Even	even	RB
cord-347472-n6811ens	240	2	commercial	commercial	JJ
cord-347472-n6811ens	240	3	offerings	offering	NNS
cord-347472-n6811ens	240	4	"	"	''
cord-347472-n6811ens	240	5	reference	reference	NN
cord-347472-n6811ens	240	6	RNA	rna	NN
cord-347472-n6811ens	240	7	"	"	''
cord-347472-n6811ens	240	8	can	can	MD
cord-347472-n6811ens	240	9	be	be	VB
cord-347472-n6811ens	240	10	contaminated	contaminate	VBN
cord-347472-n6811ens	240	11	with	with	IN
cord-347472-n6811ens	240	12	low	low	JJ
cord-347472-n6811ens	240	13	,	,	,
cord-347472-n6811ens	240	14	but	but	CC
cord-347472-n6811ens	240	15	interfering	interfering	JJ
cord-347472-n6811ens	240	16	,	,	,
cord-347472-n6811ens	240	17	levels	level	NNS
cord-347472-n6811ens	240	18	of	of	IN
cord-347472-n6811ens	240	19	human	human	JJ
cord-347472-n6811ens	240	20	DNA	dna	NN
cord-347472-n6811ens	240	21	.	.	.
cord-347472-n6811ens	241	1	Quantigene	Quantigene	NNP
cord-347472-n6811ens	241	2	human	human	JJ
cord-347472-n6811ens	241	3	reference	reference	NN
cord-347472-n6811ens	241	4	RNA	RNA	NNP
cord-347472-n6811ens	241	5	(	(	-LRB-
cord-347472-n6811ens	241	6	Life	Life	NNP
cord-347472-n6811ens	241	7	Technologies	Technologies	NNPS
cord-347472-n6811ens	241	8	)	)	-RRB-
cord-347472-n6811ens	241	9	is	be	VBZ
cord-347472-n6811ens	241	10	specified	specify	VBN
cord-347472-n6811ens	241	11	as	as	IN
cord-347472-n6811ens	241	12	a	a	DT
cord-347472-n6811ens	241	13	mixture	mixture	NN
cord-347472-n6811ens	241	14	of	of	IN
cord-347472-n6811ens	241	15	DNasetreated	dnasetreated	JJ
cord-347472-n6811ens	241	16	RNAs	rna	NNS
cord-347472-n6811ens	241	17	from	from	IN
cord-347472-n6811ens	241	18	multiple	multiple	JJ
cord-347472-n6811ens	241	19	cell	cell	NN
cord-347472-n6811ens	241	20	lines	line	NNS
cord-347472-n6811ens	241	21	.	.	.
cord-347472-n6811ens	242	1	In	in	IN
cord-347472-n6811ens	242	2	practice	practice	NN
cord-347472-n6811ens	242	3	,	,	,
cord-347472-n6811ens	242	4	this	this	DT
cord-347472-n6811ens	242	5	material	material	NN
cord-347472-n6811ens	242	6	continues	continue	VBZ
cord-347472-n6811ens	242	7	to	to	TO
cord-347472-n6811ens	242	8	generate	generate	VB
cord-347472-n6811ens	242	9	qPCR	qpcr	NN
cord-347472-n6811ens	242	10	signals	signal	NNS
cord-347472-n6811ens	242	11	both	both	DT
cord-347472-n6811ens	242	12	using	use	VBG
cord-347472-n6811ens	242	13	a	a	DT
cord-347472-n6811ens	242	14	Taq	taq	NN
cord-347472-n6811ens	242	15	-	-	HYPH
cord-347472-n6811ens	242	16	only	only	RB
cord-347472-n6811ens	242	17	,	,	,
cord-347472-n6811ens	242	18	non	non	JJ
cord-347472-n6811ens	242	19	-	-	JJ
cord-347472-n6811ens	242	20	reverse	reverse	JJ
cord-347472-n6811ens	242	21	transcribing	transcribing	NN
cord-347472-n6811ens	242	22	,	,	,
cord-347472-n6811ens	242	23	mastermix	mastermix	NNP
cord-347472-n6811ens	242	24	and	and	CC
cord-347472-n6811ens	242	25	a	a	DT
cord-347472-n6811ens	242	26	one	one	CD
cord-347472-n6811ens	242	27	-	-	HYPH
cord-347472-n6811ens	242	28	step	step	NN
cord-347472-n6811ens	242	29	RT	RT	NNP
cord-347472-n6811ens	242	30	-	-	HYPH
cord-347472-n6811ens	242	31	PCR	PCR	NNP
cord-347472-n6811ens	242	32	mix	mix	NN
cord-347472-n6811ens	242	33	following	follow	VBG
cord-347472-n6811ens	242	34	RNase	RNase	NNP
cord-347472-n6811ens	242	35	A	a	DT
cord-347472-n6811ens	242	36	treatment	treatment	NN
cord-347472-n6811ens	242	37	(	(	-LRB-
cord-347472-n6811ens	242	38	Figs	fig	NNS
cord-347472-n6811ens	242	39	.	.	.
cord-347472-n6811ens	242	40	3	3	CD
cord-347472-n6811ens	242	41	and	and	CC
cord-347472-n6811ens	242	42	S2	S2	NNP
cord-347472-n6811ens	242	43	)	)	-RRB-
cord-347472-n6811ens	242	44	.	.	.
cord-347472-n6811ens	243	1	Ct	Ct	NNP
cord-347472-n6811ens	243	2	is	be	VBZ
cord-347472-n6811ens	243	3	a	a	DT
cord-347472-n6811ens	243	4	unitless	unitless	JJ
cord-347472-n6811ens	243	5	measurement	measurement	NN
cord-347472-n6811ens	243	6	and	and	CC
cord-347472-n6811ens	243	7	should	should	MD
cord-347472-n6811ens	243	8	be	be	VB
cord-347472-n6811ens	243	9	used	use	VBN
cord-347472-n6811ens	243	10	with	with	IN
cord-347472-n6811ens	243	11	care	care	NN
cord-347472-n6811ens	243	12	when	when	WRB
cord-347472-n6811ens	243	13	discussing	discuss	VBG
cord-347472-n6811ens	243	14	quantity	quantity	NN
cord-347472-n6811ens	243	15	.	.	.
cord-347472-n6811ens	243	16	.	.	.
cord-347472-n6811ens	244	1	qPCR	qPCR	NNP
cord-347472-n6811ens	244	2	is	be	VBZ
cord-347472-n6811ens	244	3	able	able	JJ
cord-347472-n6811ens	244	4	to	to	TO
cord-347472-n6811ens	244	5	operate	operate	VB
cord-347472-n6811ens	244	6	in	in	IN
cord-347472-n6811ens	244	7	qualitative	qualitative	JJ
cord-347472-n6811ens	244	8	,	,	,
cord-347472-n6811ens	244	9	semi	semi	JJ
cord-347472-n6811ens	244	10	-	-	JJ
cord-347472-n6811ens	244	11	quantitative	quantitative	JJ
cord-347472-n6811ens	244	12	,	,	,
cord-347472-n6811ens	244	13	or	or	CC
cord-347472-n6811ens	244	14	fully	fully	RB
cord-347472-n6811ens	244	15	quantitative	quantitative	JJ
cord-347472-n6811ens	244	16	modes	mode	NNS
cord-347472-n6811ens	244	17	,	,	,
cord-347472-n6811ens	244	18	depending	depend	VBG
cord-347472-n6811ens	244	19	on	on	IN
cord-347472-n6811ens	244	20	assay	assay	NN
cord-347472-n6811ens	244	21	design	design	NN
cord-347472-n6811ens	244	22	.	.	.
cord-347472-n6811ens	245	1	Currently	currently	RB
cord-347472-n6811ens	245	2	deployed	deploy	VBN
cord-347472-n6811ens	245	3	assays	assay	NNS
cord-347472-n6811ens	245	4	are	be	VBP
cord-347472-n6811ens	245	5	effectively	effectively	RB
cord-347472-n6811ens	245	6	qualitative	qualitative	JJ
cord-347472-n6811ens	246	1	(	(	-LRB-
cord-347472-n6811ens	246	2	Yes	yes	UH
cord-347472-n6811ens	246	3	/	/	,
cord-347472-n6811ens	246	4	no	no	UH
cord-347472-n6811ens	246	5	.	.	.
cord-347472-n6811ens	247	1	Above	above	IN
cord-347472-n6811ens	247	2	or	or	CC
cord-347472-n6811ens	247	3	below	below	IN
cord-347472-n6811ens	247	4	acceptable	acceptable	JJ
cord-347472-n6811ens	247	5	Ct	Ct	NNP
cord-347472-n6811ens	247	6	)	)	-RRB-
cord-347472-n6811ens	247	7	.	.	.
cord-347472-n6811ens	248	1	Throughout	throughout	IN
cord-347472-n6811ens	248	2	this	this	DT
cord-347472-n6811ens	248	3	manuscript	manuscript	NN
cord-347472-n6811ens	248	4	,	,	,
cord-347472-n6811ens	248	5	absolute	absolute	JJ
cord-347472-n6811ens	248	6	Ct	ct	JJ
cord-347472-n6811ens	248	7	values	value	NNS
cord-347472-n6811ens	248	8	and	and	CC
cord-347472-n6811ens	248	9	difference	difference	NN
cord-347472-n6811ens	248	10	in	in	IN
cord-347472-n6811ens	248	11	Ct	Ct	NNP
cord-347472-n6811ens	248	12	between	between	IN
cord-347472-n6811ens	248	13	samples	sample	NNS
cord-347472-n6811ens	248	14	are	be	VBP
cord-347472-n6811ens	248	15	used	use	VBN
cord-347472-n6811ens	248	16	as	as	IN
cord-347472-n6811ens	248	17	a	a	DT
cord-347472-n6811ens	248	18	proxy	proxy	NN
cord-347472-n6811ens	248	19	of	of	IN
cord-347472-n6811ens	248	20	inverse(log2	inverse(log2	NNP
cord-347472-n6811ens	248	21	)	)	-RRB-
cord-347472-n6811ens	248	22	amount	amount	NN
cord-347472-n6811ens	248	23	of	of	IN
cord-347472-n6811ens	248	24	input	input	NN
cord-347472-n6811ens	248	25	material	material	NN
cord-347472-n6811ens	248	26	or	or	CC
cord-347472-n6811ens	248	27	differences	difference	NNS
cord-347472-n6811ens	248	28	thereof	thereof	RB
cord-347472-n6811ens	248	29	between	between	IN
cord-347472-n6811ens	248	30	samples	sample	NNS
cord-347472-n6811ens	248	31	.	.	.
cord-347472-n6811ens	249	1	Comparisons	comparison	NNS
cord-347472-n6811ens	249	2	are	be	VBP
cord-347472-n6811ens	249	3	restricted	restrict	VBN
cord-347472-n6811ens	249	4	to	to	IN
cord-347472-n6811ens	249	5	same	same	JJ
cord-347472-n6811ens	249	6	-	-	HYPH
cord-347472-n6811ens	249	7	probe	probe	NN
cord-347472-n6811ens	249	8	,	,	,
cord-347472-n6811ens	249	9	same	same	JJ
cord-347472-n6811ens	249	10	-	-	HYPH
cord-347472-n6811ens	249	11	instrument	instrument	NN
cord-347472-n6811ens	249	12	,	,	,
cord-347472-n6811ens	249	13	same	same	JJ
cord-347472-n6811ens	249	14	(	(	-LRB-
cord-347472-n6811ens	249	15	or	or	CC
cord-347472-n6811ens	249	16	similar	similar	JJ
cord-347472-n6811ens	249	17	)	)	-RRB-
cord-347472-n6811ens	249	18	reagent	reagent	NN
cord-347472-n6811ens	249	19	.	.	.
cord-347472-n6811ens	250	1	Calibration	calibration	NN
cord-347472-n6811ens	250	2	of	of	IN
cord-347472-n6811ens	250	3	Ct:[nucleic	Ct:[nucleic	NNP
cord-347472-n6811ens	250	4	acid	acid	NN
cord-347472-n6811ens	250	5	]	]	-RRB-
cord-347472-n6811ens	250	6	was	be	VBD
cord-347472-n6811ens	250	7	performed	perform	VBN
cord-347472-n6811ens	250	8	using	use	VBG
cord-347472-n6811ens	250	9	dilutions	dilution	NNS
cord-347472-n6811ens	250	10	of	of	IN
cord-347472-n6811ens	250	11	commercial	commercial	JJ
cord-347472-n6811ens	250	12	standards	standard	NNS
cord-347472-n6811ens	250	13	.	.	.
cord-347472-n6811ens	251	1	An	an	DT
cord-347472-n6811ens	251	2	exception	exception	NN
cord-347472-n6811ens	251	3	to	to	IN
cord-347472-n6811ens	251	4	this	this	DT
cord-347472-n6811ens	251	5	conservative	conservative	JJ
cord-347472-n6811ens	251	6	approach	approach	NN
cord-347472-n6811ens	251	7	is	be	VBZ
cord-347472-n6811ens	251	8	the	the	DT
cord-347472-n6811ens	251	9	use	use	NN
cord-347472-n6811ens	251	10	of	of	IN
cord-347472-n6811ens	251	11	the	the	DT
cord-347472-n6811ens	251	12	"	"	``
cord-347472-n6811ens	251	13	40	40	CD
cord-347472-n6811ens	251	14	cycle	cycle	NN
cord-347472-n6811ens	251	15	"	"	''
cord-347472-n6811ens	251	16	threshold	threshold	NN
cord-347472-n6811ens	251	17	specified	specify	VBN
cord-347472-n6811ens	251	18	in	in	IN
cord-347472-n6811ens	251	19	the	the	DT
cord-347472-n6811ens	251	20	CDC	CDC	NNP
cord-347472-n6811ens	251	21	EUA	EUA	NNP
cord-347472-n6811ens	251	22	.	.	.
cord-347472-n6811ens	252	1	Our	-PRON-	PRP$
cord-347472-n6811ens	252	2	results	result	NNS
cord-347472-n6811ens	252	3	err	err	VBP
cord-347472-n6811ens	252	4	on	on	IN
cord-347472-n6811ens	252	5	the	the	DT
cord-347472-n6811ens	252	6	side	side	NN
cord-347472-n6811ens	252	7	of	of	IN
cord-347472-n6811ens	252	8	underestimating	underestimate	VBG
cord-347472-n6811ens	252	9	the	the	DT
cord-347472-n6811ens	252	10	extreme	extreme	JJ
cord-347472-n6811ens	252	11	sensitivity	sensitivity	NN
cord-347472-n6811ens	252	12	of	of	IN
cord-347472-n6811ens	252	13	the	the	DT
cord-347472-n6811ens	252	14	primary	primary	JJ
cord-347472-n6811ens	252	15	CDC	CDC	NNP
cord-347472-n6811ens	252	16	design	design	NN
cord-347472-n6811ens	252	17	.	.	.
cord-347472-n6811ens	253	1	The	the	DT
cord-347472-n6811ens	253	2	Applied	Applied	NNP
cord-347472-n6811ens	253	3	Biosystems	Biosystems	NNP
cord-347472-n6811ens	253	4	Viia7	Viia7	NNP
cord-347472-n6811ens	253	5	384w	384w	CD
cord-347472-n6811ens	253	6	instrument	instrument	NN
cord-347472-n6811ens	253	7	used	use	VBN
cord-347472-n6811ens	253	8	here	here	RB
cord-347472-n6811ens	253	9	is	be	VBZ
cord-347472-n6811ens	253	10	moderately	moderately	RB
cord-347472-n6811ens	253	11	less	less	RBR
cord-347472-n6811ens	253	12	sensitive	sensitive	JJ
cord-347472-n6811ens	253	13	(	(	-LRB-
cord-347472-n6811ens	253	14	higher	high	JJR
cord-347472-n6811ens	253	15	Ct	Ct	NNP
cord-347472-n6811ens	253	16	)	)	-RRB-
cord-347472-n6811ens	253	17	for	for	IN
cord-347472-n6811ens	253	18	a	a	DT
cord-347472-n6811ens	253	19	given	give	VBN
cord-347472-n6811ens	253	20	reaction	reaction	NN
cord-347472-n6811ens	253	21	composition	composition	NN
cord-347472-n6811ens	253	22	and	and	CC
cord-347472-n6811ens	253	23	volume	volume	NN
cord-347472-n6811ens	253	24	than	than	IN
cord-347472-n6811ens	253	25	a	a	DT
cord-347472-n6811ens	253	26	96w	96w	CD
cord-347472-n6811ens	253	27	FAST	fast	JJ
cord-347472-n6811ens	253	28	reaction	reaction	NN
cord-347472-n6811ens	253	29	block	block	NN
cord-347472-n6811ens	253	30	in	in	IN
cord-347472-n6811ens	253	31	the	the	DT
cord-347472-n6811ens	253	32	same	same	JJ
cord-347472-n6811ens	253	33	instrument	instrument	NN
cord-347472-n6811ens	253	34	(	(	-LRB-
cord-347472-n6811ens	253	35	information	information	NN
cord-347472-n6811ens	253	36	obtained	obtain	VBN
cord-347472-n6811ens	253	37	from	from	IN
cord-347472-n6811ens	253	38	Life	Life	NNP
cord-347472-n6811ens	253	39	Technologies	Technologies	NNP
cord-347472-n6811ens	253	40	technical	technical	JJ
cord-347472-n6811ens	253	41	support	support	NN
cord-347472-n6811ens	253	42	,	,	,
cord-347472-n6811ens	253	43	2020/05	2020/05	CD
cord-347472-n6811ens	253	44	)	)	-RRB-
cord-347472-n6811ens	253	45	.	.	.
cord-347472-n6811ens	254	1	For	for	IN
cord-347472-n6811ens	254	2	a	a	DT
cord-347472-n6811ens	254	3	representative	representative	JJ
cord-347472-n6811ens	254	4	instrument	instrument	NN
cord-347472-n6811ens	254	5	,	,	,
cord-347472-n6811ens	254	6	the	the	DT
cord-347472-n6811ens	254	7	96w	96w	CD
cord-347472-n6811ens	254	8	FAST	FAST	NNP
cord-347472-n6811ens	254	9	block	block	NN
cord-347472-n6811ens	254	10	Ct	Ct	NNP
cord-347472-n6811ens	254	11	was	be	VBD
cord-347472-n6811ens	254	12	approximately	approximately	RB
cord-347472-n6811ens	254	13	0.6	0.6	CD
cord-347472-n6811ens	254	14	cycles	cycle	NNS
cord-347472-n6811ens	254	15	lower	lower	RBR
cord-347472-n6811ens	254	16	across	across	IN
cord-347472-n6811ens	254	17	the	the	DT
cord-347472-n6811ens	254	18	dilution	dilution	NN
cord-347472-n6811ens	254	19	range	range	NN
cord-347472-n6811ens	254	20	.	.	.
cord-347472-n6811ens	255	1	Assays	assay	NNS
cord-347472-n6811ens	255	2	should	should	MD
cord-347472-n6811ens	255	3	be	be	VB
cord-347472-n6811ens	255	4	improved	improve	VBN
cord-347472-n6811ens	255	5	when	when	WRB
cord-347472-n6811ens	255	6	feasible	feasible	JJ
cord-347472-n6811ens	255	7	,	,	,
cord-347472-n6811ens	255	8	not	not	RB
cord-347472-n6811ens	255	9	rubber	rubber	NN
cord-347472-n6811ens	255	10	-	-	HYPH
cord-347472-n6811ens	255	11	stamped	stamp	VBN
cord-347472-n6811ens	255	12	and	and	CC
cord-347472-n6811ens	255	13	set	set	VBN
cord-347472-n6811ens	255	14	in	in	IN
cord-347472-n6811ens	255	15	stone	stone	NN
cord-347472-n6811ens	255	16	.	.	.
cord-347472-n6811ens	256	1	I	-PRON-	PRP
cord-347472-n6811ens	256	2	alerted	alert	VBD
cord-347472-n6811ens	256	3	CDC	CDC	NNP
cord-347472-n6811ens	256	4	that	that	IN
cord-347472-n6811ens	256	5	their	-PRON-	PRP$
cord-347472-n6811ens	256	6	EUA	EUA	NNP
cord-347472-n6811ens	256	7	-	-	HYPH
cord-347472-n6811ens	256	8	specified	specify	VBN
cord-347472-n6811ens	256	9	suffered	suffer	VBD
cord-347472-n6811ens	256	10	from	from	IN
cord-347472-n6811ens	256	11	a	a	DT
cord-347472-n6811ens	256	12	potential	potential	JJ
cord-347472-n6811ens	256	13	false	false	JJ
cord-347472-n6811ens	256	14	-	-	HYPH
cord-347472-n6811ens	256	15	negative	negative	JJ
cord-347472-n6811ens	256	16	on	on	IN
cord-347472-n6811ens	256	17	March	March	NNP
cord-347472-n6811ens	256	18	20	20	CD
cord-347472-n6811ens	256	19	,	,	,
cord-347472-n6811ens	256	20	2020	2020	CD
cord-347472-n6811ens	256	21	(	(	-LRB-
cord-347472-n6811ens	256	22	attached	attach	VBN
cord-347472-n6811ens	256	23	below	below	RB
cord-347472-n6811ens	256	24	)	)	-RRB-
cord-347472-n6811ens	256	25	.	.	.
cord-347472-n6811ens	257	1	Their	-PRON-	PRP$
cord-347472-n6811ens	257	2	response	response	NN
cord-347472-n6811ens	257	3	,	,	,
cord-347472-n6811ens	257	4	sent	send	VBD
cord-347472-n6811ens	257	5	thirty	thirty	CD
cord-347472-n6811ens	257	6	-	-	HYPH
cord-347472-n6811ens	257	7	six	six	CD
cord-347472-n6811ens	257	8	days	day	NNS
cord-347472-n6811ens	257	9	later	later	RB
cord-347472-n6811ens	257	10	(	(	-LRB-
cord-347472-n6811ens	257	11	also	also	RB
cord-347472-n6811ens	257	12	attached	attach	VBN
cord-347472-n6811ens	257	13	)	)	-RRB-
cord-347472-n6811ens	257	14	,	,	,
cord-347472-n6811ens	257	15	dismissed	dismiss	VBD
cord-347472-n6811ens	257	16	my	-PRON-	PRP$
cord-347472-n6811ens	257	17	concerns	concern	NNS
cord-347472-n6811ens	257	18	at	at	IN
cord-347472-n6811ens	257	19	multiple	multiple	JJ
cord-347472-n6811ens	257	20	levels	level	NNS
cord-347472-n6811ens	257	21	.	.	.
cord-347472-n6811ens	258	1	One	one	CD
cord-347472-n6811ens	258	2	:	:	:
cord-347472-n6811ens	258	3	there	there	EX
cord-347472-n6811ens	258	4	had	have	VBD
cord-347472-n6811ens	258	5	not	not	RB
cord-347472-n6811ens	258	6	been	be	VBN
cord-347472-n6811ens	258	7	widespread	widespread	JJ
cord-347472-n6811ens	258	8	reporting	reporting	NN
cord-347472-n6811ens	258	9	of	of	IN
cord-347472-n6811ens	258	10	false	false	JJ
cord-347472-n6811ens	258	11	negatives	negative	NNS
cord-347472-n6811ens	258	12	from	from	IN
cord-347472-n6811ens	258	13	this	this	DT
cord-347472-n6811ens	258	14	test	test	NN
cord-347472-n6811ens	258	15	.	.	.
cord-347472-n6811ens	259	1	Two	two	CD
cord-347472-n6811ens	259	2	:	:	:
cord-347472-n6811ens	259	3	CDC	CDC	NNP
cord-347472-n6811ens	259	4	's	's	POS
cord-347472-n6811ens	259	5	clinical	clinical	JJ
cord-347472-n6811ens	259	6	algorithms	algorithm	NNS
cord-347472-n6811ens	259	7	state	state	NN
cord-347472-n6811ens	259	8	that	that	IN
cord-347472-n6811ens	259	9	negative	negative	JJ
cord-347472-n6811ens	259	10	-	-	HYPH
cord-347472-n6811ens	259	11	testing	testing	NN
cord-347472-n6811ens	259	12	patients	patient	NNS
cord-347472-n6811ens	259	13	should	should	MD
cord-347472-n6811ens	259	14	be	be	VB
cord-347472-n6811ens	259	15	retested	reteste	VBN
cord-347472-n6811ens	259	16	and	and	CC
cord-347472-n6811ens	259	17	that	that	IN
cord-347472-n6811ens	259	18	clinical	clinical	JJ
cord-347472-n6811ens	259	19	management	management	NN
cord-347472-n6811ens	259	20	should	should	MD
cord-347472-n6811ens	259	21	not	not	RB
cord-347472-n6811ens	259	22	rely	rely	VB
cord-347472-n6811ens	259	23	solely	solely	RB
cord-347472-n6811ens	259	24	on	on	IN
cord-347472-n6811ens	259	25	this	this	DT
cord-347472-n6811ens	259	26	test	test	NN
cord-347472-n6811ens	259	27	.	.	.
cord-347472-n6811ens	260	1	Three	three	CD
cord-347472-n6811ens	260	2	:	:	:
cord-347472-n6811ens	260	3	the	the	DT
cord-347472-n6811ens	260	4	FDA	FDA	NNP
cord-347472-n6811ens	260	5	had	have	VBD
cord-347472-n6811ens	260	6	approved	approve	VBN
cord-347472-n6811ens	260	7	the	the	DT
cord-347472-n6811ens	260	8	test	test	NN
cord-347472-n6811ens	260	9	,	,	,
cord-347472-n6811ens	260	10	making	make	VBG
cord-347472-n6811ens	260	11	it	-PRON-	PRP
cord-347472-n6811ens	260	12	,	,	,
cord-347472-n6811ens	260	13	de	de	FW
cord-347472-n6811ens	260	14	jure	jure	NNP
cord-347472-n6811ens	260	15	,	,	,
cord-347472-n6811ens	260	16	acceptable	acceptable	JJ
cord-347472-n6811ens	260	17	.	.	.
cord-347472-n6811ens	261	1	Each	each	DT
cord-347472-n6811ens	261	2	of	of	IN
cord-347472-n6811ens	261	3	the	the	DT
cord-347472-n6811ens	261	4	CDC	CDC	NNP
cord-347472-n6811ens	261	5	's	's	POS
cord-347472-n6811ens	261	6	arguments	argument	NNS
cord-347472-n6811ens	261	7	is	be	VBZ
cord-347472-n6811ens	261	8	fallacious	fallacious	JJ
cord-347472-n6811ens	261	9	.	.	.
cord-347472-n6811ens	262	1	False	false	JJ
cord-347472-n6811ens	262	2	negatives	negative	NNS
cord-347472-n6811ens	262	3	are	be	VBP
cord-347472-n6811ens	262	4	inherently	inherently	RB
cord-347472-n6811ens	262	5	difficult	difficult	JJ
cord-347472-n6811ens	262	6	to	to	TO
cord-347472-n6811ens	262	7	catch	catch	VB
cord-347472-n6811ens	262	8	,	,	,
cord-347472-n6811ens	262	9	and	and	CC
cord-347472-n6811ens	262	10	identification	identification	NN
cord-347472-n6811ens	262	11	of	of	IN
cord-347472-n6811ens	262	12	true	true	JJ
cord-347472-n6811ens	262	13	positives	positive	NNS
cord-347472-n6811ens	262	14	is	be	VBZ
cord-347472-n6811ens	262	15	a	a	DT
cord-347472-n6811ens	262	16	challenging	challenge	VBG
cord-347472-n6811ens	262	17	bootstrap	bootstrap	NN
cord-347472-n6811ens	262	18	problem	problem	NN
cord-347472-n6811ens	262	19	.	.	.
cord-347472-n6811ens	263	1	The	the	DT
cord-347472-n6811ens	263	2	idea	idea	NN
cord-347472-n6811ens	263	3	of	of	IN
cord-347472-n6811ens	263	4	"	"	``
cord-347472-n6811ens	263	5	re	re	NN
cord-347472-n6811ens	263	6	-	-	NN
cord-347472-n6811ens	263	7	test	test	NN
cord-347472-n6811ens	263	8	if	if	IN
cord-347472-n6811ens	263	9	you	-PRON-	PRP
cord-347472-n6811ens	263	10	're	be	VBP
cord-347472-n6811ens	263	11	uncertain	uncertain	JJ
cord-347472-n6811ens	263	12	"	"	''
cord-347472-n6811ens	263	13	is	be	VBZ
cord-347472-n6811ens	263	14	tone	tone	NN
cord-347472-n6811ens	263	15	deaf	deaf	JJ
cord-347472-n6811ens	263	16	in	in	IN
cord-347472-n6811ens	263	17	the	the	DT
cord-347472-n6811ens	263	18	context	context	NN
cord-347472-n6811ens	263	19	of	of	IN
cord-347472-n6811ens	263	20	widespread	widespread	JJ
cord-347472-n6811ens	263	21	shortages	shortage	NNS
cord-347472-n6811ens	263	22	of	of	IN
cord-347472-n6811ens	263	23	testing	test	VBG
cord-347472-n6811ens	263	24	reagents	reagent	NNS
cord-347472-n6811ens	263	25	and	and	CC
cord-347472-n6811ens	263	26	long	long	JJ
cord-347472-n6811ens	263	27	backlogs	backlog	NNS
cord-347472-n6811ens	263	28	for	for	IN
cord-347472-n6811ens	263	29	sample	sample	NN
cord-347472-n6811ens	263	30	processing	processing	NN
cord-347472-n6811ens	263	31	,	,	,
cord-347472-n6811ens	263	32	not	not	RB
cord-347472-n6811ens	263	33	to	to	TO
cord-347472-n6811ens	263	34	mention	mention	VB
cord-347472-n6811ens	263	35	the	the	DT
cord-347472-n6811ens	263	36	moderately	moderately	RB
cord-347472-n6811ens	263	37	invasive	invasive	JJ
cord-347472-n6811ens	263	38	nature	nature	NN
cord-347472-n6811ens	263	39	of	of	IN
cord-347472-n6811ens	263	40	nasopharyngeal	nasopharyngeal	JJ
cord-347472-n6811ens	263	41	sampling	sampling	NN
cord-347472-n6811ens	263	42	.	.	.
cord-347472-n6811ens	264	1	Appealing	appeal	VBG
cord-347472-n6811ens	264	2	to	to	IN
cord-347472-n6811ens	264	3	FDA	FDA	NNP
cord-347472-n6811ens	264	4	's	's	POS
cord-347472-n6811ens	264	5	authority	authority	NN
cord-347472-n6811ens	264	6	may	may	MD
cord-347472-n6811ens	264	7	be	be	VB
cord-347472-n6811ens	264	8	legally	legally	RB
cord-347472-n6811ens	264	9	satisfying	satisfying	JJ
cord-347472-n6811ens	264	10	,	,	,
cord-347472-n6811ens	264	11	but	but	CC
cord-347472-n6811ens	264	12	does	do	VBZ
cord-347472-n6811ens	264	13	nothing	nothing	NN
cord-347472-n6811ens	264	14	to	to	TO
cord-347472-n6811ens	264	15	actually	actually	RB
cord-347472-n6811ens	264	16	address	address	VB
cord-347472-n6811ens	264	17	issues	issue	NNS
cord-347472-n6811ens	264	18	of	of	IN
cord-347472-n6811ens	264	19	scientific	scientific	JJ
cord-347472-n6811ens	264	20	merit	merit	NN
cord-347472-n6811ens	264	21	.	.	.
cord-347472-n6811ens	265	1	We	-PRON-	PRP
cord-347472-n6811ens	265	2	will	will	MD
cord-347472-n6811ens	265	3	never	never	RB
cord-347472-n6811ens	265	4	know	know	VB
cord-347472-n6811ens	265	5	how	how	WRB
cord-347472-n6811ens	265	6	many	many	JJ
cord-347472-n6811ens	265	7	virus	virus	NN
cord-347472-n6811ens	265	8	-	-	HYPH
cord-347472-n6811ens	265	9	positive	positive	JJ
cord-347472-n6811ens	265	10	patients	patient	NNS
cord-347472-n6811ens	265	11	have	have	VBP
cord-347472-n6811ens	265	12	been	be	VBN
cord-347472-n6811ens	265	13	given	give	VBN
cord-347472-n6811ens	265	14	what	what	WP
cord-347472-n6811ens	265	15	amounts	amount	VBZ
cord-347472-n6811ens	265	16	to	to	IN
cord-347472-n6811ens	265	17	an	an	DT
cord-347472-n6811ens	265	18	"	"	``
cord-347472-n6811ens	265	19	all	all	RB
cord-347472-n6811ens	265	20	clear	clear	JJ
cord-347472-n6811ens	265	21	"	"	''
cord-347472-n6811ens	265	22	due	due	JJ
cord-347472-n6811ens	265	23	to	to	IN
cord-347472-n6811ens	265	24	false	false	JJ
cord-347472-n6811ens	265	25	-	-	HYPH
cord-347472-n6811ens	265	26	negatives	negative	NNS
cord-347472-n6811ens	265	27	generated	generate	VBN
cord-347472-n6811ens	265	28	by	by	IN
cord-347472-n6811ens	265	29	this	this	DT
cord-347472-n6811ens	265	30	flawed	flawed	JJ
cord-347472-n6811ens	265	31	control	control	NN
cord-347472-n6811ens	265	32	design	design	NN
cord-347472-n6811ens	265	33	.	.	.
cord-347472-n6811ens	266	1	The	the	DT
cord-347472-n6811ens	266	2	potential	potential	NN
cord-347472-n6811ens	266	3	for	for	IN
cord-347472-n6811ens	266	4	misdiagnosis	misdiagnosis	NN
cord-347472-n6811ens	266	5	is	be	VBZ
cord-347472-n6811ens	266	6	real	real	JJ
cord-347472-n6811ens	266	7	,	,	,
cord-347472-n6811ens	266	8	and	and	CC
cord-347472-n6811ens	266	9	there	there	EX
cord-347472-n6811ens	266	10	is	be	VBZ
cord-347472-n6811ens	266	11	a	a	DT
cord-347472-n6811ens	266	12	window	window	NN
cord-347472-n6811ens	266	13	to	to	TO
cord-347472-n6811ens	266	14	make	make	VB
cord-347472-n6811ens	266	15	an	an	DT
cord-347472-n6811ens	266	16	important	important	JJ
cord-347472-n6811ens	266	17	assay	assay	NN
cord-347472-n6811ens	266	18	work	work	NN
cord-347472-n6811ens	266	19	as	as	IN
cord-347472-n6811ens	266	20	it	-PRON-	PRP
cord-347472-n6811ens	266	21	was	be	VBD
cord-347472-n6811ens	266	22	intended	intend	VBN
cord-347472-n6811ens	266	23	.	.	.
cord-347472-n6811ens	267	1	The	the	DT
cord-347472-n6811ens	267	2	alternative	alternative	NN
cord-347472-n6811ens	267	3	would	would	MD
cord-347472-n6811ens	267	4	be	be	VB
cord-347472-n6811ens	267	5	for	for	IN
cord-347472-n6811ens	267	6	CDC	CDC	NNP
cord-347472-n6811ens	267	7	to	to	TO
cord-347472-n6811ens	267	8	say	say	VB
cord-347472-n6811ens	267	9	"	"	``
cord-347472-n6811ens	267	10	our	-PRON-	PRP$
cord-347472-n6811ens	267	11	test	test	NN
cord-347472-n6811ens	267	12	does	do	VBZ
cord-347472-n6811ens	267	13	n't	not	RB
cord-347472-n6811ens	267	14	work	work	VB
cord-347472-n6811ens	267	15	as	as	IN
cord-347472-n6811ens	267	16	we	-PRON-	PRP
cord-347472-n6811ens	267	17	intended	intend	VBD
cord-347472-n6811ens	267	18	;	;	:
cord-347472-n6811ens	267	19	if	if	IN
cord-347472-n6811ens	267	20	you	-PRON-	PRP
cord-347472-n6811ens	267	21	are	be	VBP
cord-347472-n6811ens	267	22	worried	worried	JJ
cord-347472-n6811ens	267	23	that	that	IN
cord-347472-n6811ens	267	24	you	-PRON-	PRP
cord-347472-n6811ens	267	25	're	be	VBP
cord-347472-n6811ens	267	26	sick	sick	JJ
cord-347472-n6811ens	267	27	and	and	CC
cord-347472-n6811ens	267	28	your	-PRON-	PRP$
cord-347472-n6811ens	267	29	test	test	NN
cord-347472-n6811ens	267	30	has	have	VBZ
cord-347472-n6811ens	267	31	come	come	VBN
cord-347472-n6811ens	267	32	back	back	RB
cord-347472-n6811ens	267	33	negative	negative	JJ
cord-347472-n6811ens	267	34	,	,	,
cord-347472-n6811ens	267	35	come	come	VB
cord-347472-n6811ens	267	36	back	back	RB
cord-347472-n6811ens	267	37	and	and	CC
cord-347472-n6811ens	267	38	be	be	VB
cord-347472-n6811ens	267	39	tested	test	VBN
cord-347472-n6811ens	267	40	again	again	RB
cord-347472-n6811ens	267	41	until	until	IN
cord-347472-n6811ens	267	42	your	-PRON-	PRP$
cord-347472-n6811ens	267	43	fears	fear	NNS
cord-347472-n6811ens	267	44	are	be	VBP
cord-347472-n6811ens	267	45	allayed	allay	VBN
cord-347472-n6811ens	267	46	,	,	,
cord-347472-n6811ens	267	47	or	or	CC
cord-347472-n6811ens	267	48	you	-PRON-	PRP
cord-347472-n6811ens	267	49	test	test	VBP
cord-347472-n6811ens	267	50	positive	positive	JJ
cord-347472-n6811ens	267	51	.	.	.
cord-347472-n6811ens	268	1	"	"	``
cord-347472-n6811ens	268	2	Fixing	fix	VBG
cord-347472-n6811ens	268	3	some	some	DT
cord-347472-n6811ens	268	4	problems	problem	NNS
cord-347472-n6811ens	268	5	is	be	VBZ
cord-347472-n6811ens	268	6	difficult	difficult	JJ
cord-347472-n6811ens	268	7	;	;	:
cord-347472-n6811ens	268	8	others	other	NNS
cord-347472-n6811ens	268	9	are	be	VBP
cord-347472-n6811ens	268	10	difficult	difficult	JJ
cord-347472-n6811ens	268	11	to	to	TO
cord-347472-n6811ens	268	12	identify	identify	VB
cord-347472-n6811ens	268	13	but	but	CC
cord-347472-n6811ens	268	14	easy	easy	JJ
cord-347472-n6811ens	268	15	to	to	TO
cord-347472-n6811ens	268	16	remedy	remedy	VB
cord-347472-n6811ens	268	17	once	once	RB
cord-347472-n6811ens	268	18	found	find	VBN
cord-347472-n6811ens	268	19	.	.	.
cord-347472-n6811ens	269	1	As	as	IN
cord-347472-n6811ens	269	2	shown	show	VBN
cord-347472-n6811ens	269	3	here	here	RB
cord-347472-n6811ens	269	4	,	,	,
cord-347472-n6811ens	269	5	this	this	DT
cord-347472-n6811ens	269	6	important	important	JJ
cord-347472-n6811ens	269	7	assay	assay	NN
cord-347472-n6811ens	269	8	can	can	MD
cord-347472-n6811ens	269	9	be	be	VB
cord-347472-n6811ens	269	10	made	make	VBN
cord-347472-n6811ens	269	11	to	to	TO
cord-347472-n6811ens	269	12	operate	operate	VB
cord-347472-n6811ens	269	13	as	as	IN
cord-347472-n6811ens	269	14	intended	intend	VBN
cord-347472-n6811ens	269	15	by	by	IN
cord-347472-n6811ens	269	16	a	a	DT
cord-347472-n6811ens	269	17	re	re	NN
cord-347472-n6811ens	269	18	-	-	NN
cord-347472-n6811ens	269	19	manufacture	manufacture	NN
cord-347472-n6811ens	269	20	of	of	IN
cord-347472-n6811ens	269	21	a	a	DT
cord-347472-n6811ens	269	22	single	single	JJ
cord-347472-n6811ens	269	23	reagent	reagent	NN
cord-347472-n6811ens	269	24	.	.	.
cord-347472-n6811ens	270	1	A	a	DT
cord-347472-n6811ens	270	2	B	B	NNP
cord-347472-n6811ens	270	3	RNA	RNA	NNP
cord-347472-n6811ens	270	4	,	,	,
cord-347472-n6811ens	270	5	but	but	CC
cord-347472-n6811ens	270	6	not	not	RB
cord-347472-n6811ens	270	7	genomic	genomic	JJ
cord-347472-n6811ens	270	8	DNA	dna	NN
cord-347472-n6811ens	270	9	,	,	,
cord-347472-n6811ens	270	10	is	be	VBZ
cord-347472-n6811ens	270	11	a	a	DT
cord-347472-n6811ens	270	12	productive	productive	JJ
cord-347472-n6811ens	270	13	template	template	NN
cord-347472-n6811ens	270	14	for	for	IN
cord-347472-n6811ens	270	15	reverse	reverse	JJ
cord-347472-n6811ens	270	16	-	-	HYPH
cord-347472-n6811ens	270	17	transcription	transcription	NN
cord-347472-n6811ens	270	18	-	-	HYPH
cord-347472-n6811ens	270	19	qPCR	qpcr	NN
cord-347472-n6811ens	270	20	amplification	amplification	NN
cord-347472-n6811ens	270	21	using	use	VBG
cord-347472-n6811ens	270	22	any	any	DT
cord-347472-n6811ens	270	23	of	of	IN
cord-347472-n6811ens	270	24	the	the	DT
cord-347472-n6811ens	270	25	five	five	CD
cord-347472-n6811ens	270	26	tested	test	VBN
cord-347472-n6811ens	270	27	transcript	transcript	NN
cord-347472-n6811ens	270	28	-	-	HYPH
cord-347472-n6811ens	270	29	specific	specific	JJ
cord-347472-n6811ens	270	30	RPP30	RPP30	NNP
cord-347472-n6811ens	270	31	controls	control	NNS
cord-347472-n6811ens	270	32	.	.	.
cord-347472-n6811ens	271	1	One	one	CD
cord-347472-n6811ens	271	2	-	-	HYPH
cord-347472-n6811ens	271	3	step	step	NN
cord-347472-n6811ens	271	4	RT	RT	NNP
cord-347472-n6811ens	271	5	-	-	HYPH
cord-347472-n6811ens	271	6	qPCR	qPCR	NNP
cord-347472-n6811ens	271	7	was	be	VBD
cord-347472-n6811ens	271	8	performed	perform	VBN
cord-347472-n6811ens	271	9	with	with	IN
cord-347472-n6811ens	271	10	the	the	DT
cord-347472-n6811ens	271	11	same	same	JJ
cord-347472-n6811ens	271	12	mastermix	mastermix	NN
cord-347472-n6811ens	271	13	used	use	VBN
cord-347472-n6811ens	271	14	for	for	IN
cord-347472-n6811ens	271	15	clinical	clinical	JJ
cord-347472-n6811ens	271	16	samples	sample	NNS
cord-347472-n6811ens	271	17	across	across	IN
cord-347472-n6811ens	271	18	a	a	DT
cord-347472-n6811ens	271	19	2-fold	2-fold	CD
cord-347472-n6811ens	271	20	dilution	dilution	NN
cord-347472-n6811ens	271	21	series	series	NN
cord-347472-n6811ens	271	22	(	(	-LRB-
cord-347472-n6811ens	271	23	Materials	Materials	NNPS
cord-347472-n6811ens	271	24	and	and	CC
cord-347472-n6811ens	271	25	Methods	Methods	NNPS
cord-347472-n6811ens	271	26	)	)	-RRB-
cord-347472-n6811ens	271	27	.	.	.
cord-347472-n6811ens	272	1	As	as	IN
cord-347472-n6811ens	272	2	expected	expect	VBN
cord-347472-n6811ens	272	3	,	,	,
cord-347472-n6811ens	272	4	pre	pre	JJ
cord-347472-n6811ens	272	5	-	-	JJ
cord-347472-n6811ens	272	6	pandemic	pandemic	JJ
cord-347472-n6811ens	272	7	commercial	commercial	JJ
cord-347472-n6811ens	272	8	reference	reference	NN
cord-347472-n6811ens	272	9	RNA	RNA	NNP
cord-347472-n6811ens	272	10	is	be	VBZ
cord-347472-n6811ens	272	11	nonreactive	nonreactive	JJ
cord-347472-n6811ens	272	12	to	to	IN
cord-347472-n6811ens	272	13	the	the	DT
cord-347472-n6811ens	272	14	SARS	SARS	NNP
cord-347472-n6811ens	272	15	-	-	HYPH
cord-347472-n6811ens	272	16	CoV-2	CoV-2	NNP
cord-347472-n6811ens	272	17	primer	primer	NN
cord-347472-n6811ens	272	18	/	/	SYM
cord-347472-n6811ens	272	19	probe	probe	NN
cord-347472-n6811ens	272	20	sets	set	NNS
cord-347472-n6811ens	272	21	.	.	.
cord-347472-n6811ens	273	1	All	all	DT
cord-347472-n6811ens	273	2	six	six	CD
cord-347472-n6811ens	273	3	primer	primer	NN
cord-347472-n6811ens	273	4	/	/	SYM
cord-347472-n6811ens	273	5	probe	probe	NN
cord-347472-n6811ens	273	6	sets	set	NNS
cord-347472-n6811ens	273	7	targeting	target	VBG
cord-347472-n6811ens	273	8	RPP30	rpp30	TO
cord-347472-n6811ens	273	9	show	show	VB
cord-347472-n6811ens	273	10	a	a	DT
cord-347472-n6811ens	273	11	strong	strong	JJ
cord-347472-n6811ens	273	12	and	and	CC
cord-347472-n6811ens	273	13	relatively	relatively	RB
cord-347472-n6811ens	273	14	linear	linear	JJ
cord-347472-n6811ens	273	15	signal	signal	NN
cord-347472-n6811ens	273	16	across	across	IN
cord-347472-n6811ens	273	17	a	a	DT
cord-347472-n6811ens	273	18	range	range	NN
cord-347472-n6811ens	273	19	of	of	IN
cord-347472-n6811ens	273	20	RNA	RNA	NNP
cord-347472-n6811ens	273	21	input	input	NN
cord-347472-n6811ens	273	22	(	(	-LRB-
cord-347472-n6811ens	273	23	A	a	NN
cord-347472-n6811ens	273	24	)	)	-RRB-
cord-347472-n6811ens	273	25	(	(	-LRB-
cord-347472-n6811ens	273	26	S1a	s1a	NN
cord-347472-n6811ens	273	27	)	)	-RRB-
cord-347472-n6811ens	273	28	.	.	.
cord-347472-n6811ens	274	1	Using	use	VBG
cord-347472-n6811ens	274	2	the	the	DT
cord-347472-n6811ens	274	3	same	same	JJ
cord-347472-n6811ens	274	4	reverse	reverse	NN
cord-347472-n6811ens	274	5	-	-	HYPH
cord-347472-n6811ens	274	6	transcriptase	transcriptase	NN
cord-347472-n6811ens	274	7	containing	contain	VBG
cord-347472-n6811ens	274	8	mastermix	mastermix	NNP
cord-347472-n6811ens	274	9	with	with	IN
cord-347472-n6811ens	274	10	mass	mass	JJ
cord-347472-n6811ens	274	11	-	-	HYPH
cord-347472-n6811ens	274	12	equivalent	equivalent	JJ
cord-347472-n6811ens	274	13	dilutions	dilution	NNS
cord-347472-n6811ens	274	14	of	of	IN
cord-347472-n6811ens	274	15	RNA	rna	NN
cord-347472-n6811ens	274	16	-	-	HYPH
cord-347472-n6811ens	274	17	free	free	JJ
cord-347472-n6811ens	274	18	genomic	genomic	JJ
cord-347472-n6811ens	274	19	DNA	dna	NN
cord-347472-n6811ens	274	20	input	input	NN
cord-347472-n6811ens	274	21	,	,	,
cord-347472-n6811ens	274	22	none	none	NN
cord-347472-n6811ens	274	23	of	of	IN
cord-347472-n6811ens	274	24	the	the	DT
cord-347472-n6811ens	274	25	transcript	transcript	NN
cord-347472-n6811ens	274	26	-	-	HYPH
cord-347472-n6811ens	274	27	specific	specific	JJ
cord-347472-n6811ens	274	28	probes	probe	NNS
cord-347472-n6811ens	274	29	generated	generate	VBD
cord-347472-n6811ens	274	30	a	a	DT
cord-347472-n6811ens	274	31	qPCR	qpcr	NN
cord-347472-n6811ens	274	32	signal	signal	NN
cord-347472-n6811ens	274	33	,	,	,
cord-347472-n6811ens	274	34	within	within	IN
cord-347472-n6811ens	274	35	50	50	CD
cord-347472-n6811ens	274	36	cycles	cycle	NNS
cord-347472-n6811ens	274	37	,	,	,
cord-347472-n6811ens	274	38	across	across	IN
cord-347472-n6811ens	274	39	a	a	DT
cord-347472-n6811ens	274	40	titration	titration	NN
cord-347472-n6811ens	274	41	of	of	IN
cord-347472-n6811ens	274	42	purified	purify	VBN
cord-347472-n6811ens	274	43	DNA	dna	NN
cord-347472-n6811ens	274	44	ranging	range	VBG
cord-347472-n6811ens	274	45	from	from	IN
cord-347472-n6811ens	274	46	20,000	20,000	CD
cord-347472-n6811ens	274	47	copies	copy	NNS
cord-347472-n6811ens	274	48	to	to	IN
cord-347472-n6811ens	274	49	19.5	19.5	CD
cord-347472-n6811ens	274	50	copies	copy	NNS
cord-347472-n6811ens	274	51	/	/	SYM
cord-347472-n6811ens	274	52	reaction	reaction	NN
cord-347472-n6811ens	274	53	.	.	.
cord-347472-n6811ens	275	1	(	(	-LRB-
cord-347472-n6811ens	275	2	B	b	NN
cord-347472-n6811ens	275	3	)	)	-RRB-
cord-347472-n6811ens	276	1	The	the	DT
cord-347472-n6811ens	276	2	CDC	CDC	NNP
cord-347472-n6811ens	276	3	-	-	HYPH
cord-347472-n6811ens	276	4	specified	specify	VBN
cord-347472-n6811ens	276	5	RP	RP	NNP
cord-347472-n6811ens	276	6	control	control	NN
cord-347472-n6811ens	276	7	generates	generate	VBZ
cord-347472-n6811ens	276	8	a	a	DT
cord-347472-n6811ens	276	9	strong	strong	JJ
cord-347472-n6811ens	276	10	signal	signal	NN
cord-347472-n6811ens	276	11	from	from	IN
cord-347472-n6811ens	276	12	genomic	genomic	JJ
cord-347472-n6811ens	276	13	DNA	dna	NN
cord-347472-n6811ens	276	14	.	.	.
cord-347472-n6811ens	277	1	Reactions	reaction	NNS
cord-347472-n6811ens	277	2	containing	contain	VBG
cord-347472-n6811ens	277	3	64ng	64ng	NN
cord-347472-n6811ens	277	4	of	of	IN
cord-347472-n6811ens	277	5	either	either	DT
cord-347472-n6811ens	277	6	total	total	JJ
cord-347472-n6811ens	277	7	RNA	RNA	NNP
cord-347472-n6811ens	277	8	or	or	CC
cord-347472-n6811ens	277	9	genomic	genomic	JJ
cord-347472-n6811ens	277	10	DNA	dna	NN
cord-347472-n6811ens	277	11	generated	generate	VBD
cord-347472-n6811ens	277	12	a	a	DT
cord-347472-n6811ens	277	13	~3	~3	JJ
cord-347472-n6811ens	277	14	cycle	cycle	NN
cord-347472-n6811ens	277	15	difference	difference	NN
cord-347472-n6811ens	277	16	for	for	IN
cord-347472-n6811ens	277	17	threshold	threshold	NN
cord-347472-n6811ens	277	18	crossing	crossing	NN
cord-347472-n6811ens	277	19	(	(	-LRB-
cord-347472-n6811ens	277	20	Supplemental	Supplemental	NNP
cord-347472-n6811ens	277	21	Text	Text	NNP
cord-347472-n6811ens	277	22	)	)	-RRB-
cord-347472-n6811ens	277	23	.	.	.
cord-347472-n6811ens	278	1	Modest	modest	JJ
cord-347472-n6811ens	278	2	baseline	baseline	NN
cord-347472-n6811ens	278	3	and	and	CC
cord-347472-n6811ens	278	4	NTC	NTC	NNP
cord-347472-n6811ens	278	5	signal	signal	NN
cord-347472-n6811ens	278	6	is	be	VBZ
cord-347472-n6811ens	278	7	due	due	JJ
cord-347472-n6811ens	278	8	to	to	IN
cord-347472-n6811ens	278	9	offsetting	offset	VBG
cord-347472-n6811ens	278	10	of	of	IN
cord-347472-n6811ens	278	11	raw	raw	JJ
cord-347472-n6811ens	278	12	qPCR	qpcr	NN
cord-347472-n6811ens	278	13	signals	signal	NNS
cord-347472-n6811ens	278	14	before	before	IN
cord-347472-n6811ens	278	15	plotting	plot	VBG
cord-347472-n6811ens	278	16	,	,	,
cord-347472-n6811ens	278	17	as	as	IN
cord-347472-n6811ens	278	18	described	describe	VBN
cord-347472-n6811ens	278	19	in	in	IN
cord-347472-n6811ens	278	20	Materials	material	NNS
cord-347472-n6811ens	278	21	and	and	CC
cord-347472-n6811ens	278	22	Methods	method	NNS
cord-347472-n6811ens	278	23	.	.	.
cord-347472-n6811ens	279	1	Positive	positive	JJ
cord-347472-n6811ens	279	2	signal	signal	NN
cord-347472-n6811ens	279	3	from	from	IN
cord-347472-n6811ens	279	4	a	a	DT
cord-347472-n6811ens	279	5	transcript	transcript	NN
cord-347472-n6811ens	279	6	-	-	HYPH
cord-347472-n6811ens	279	7	specific	specific	JJ
cord-347472-n6811ens	279	8	RPP30	RPP30	NNP
cord-347472-n6811ens	279	9	probe	probe	NN
cord-347472-n6811ens	279	10	requires	require	VBZ
cord-347472-n6811ens	279	11	both	both	CC
cord-347472-n6811ens	279	12	RNA	RNA	NNP
cord-347472-n6811ens	279	13	and	and	CC
cord-347472-n6811ens	279	14	reverse	reverse	VB
cord-347472-n6811ens	279	15	transcriptase	transcriptase	NN
cord-347472-n6811ens	279	16	(	(	-LRB-
cord-347472-n6811ens	279	17	RT	RT	NNP
cord-347472-n6811ens	279	18	)	)	-RRB-
cord-347472-n6811ens	279	19	.	.	.
cord-347472-n6811ens	280	1	The	the	DT
cord-347472-n6811ens	280	2	CDC	CDC	NNP
cord-347472-n6811ens	280	3	-	-	HYPH
cord-347472-n6811ens	280	4	specified	specify	VBN
cord-347472-n6811ens	280	5	RP	rp	NN
cord-347472-n6811ens	280	6	control	control	NN
cord-347472-n6811ens	280	7	requires	require	VBZ
cord-347472-n6811ens	280	8	neither	neither	CC
cord-347472-n6811ens	280	9	RNA	RNA	NNP
cord-347472-n6811ens	280	10	nor	nor	CC
cord-347472-n6811ens	280	11	RT	RT	NNP
cord-347472-n6811ens	280	12	when	when	WRB
cord-347472-n6811ens	280	13	genomic	genomic	NN
cord-347472-n6811ens	280	14	is	be	VBZ
cord-347472-n6811ens	280	15	present	present	JJ
cord-347472-n6811ens	280	16	.	.	.
cord-347472-n6811ens	281	1	Dilutions	dilution	NNS
cord-347472-n6811ens	281	2	of	of	IN
cord-347472-n6811ens	281	3	either	either	DT
cord-347472-n6811ens	281	4	commercial	commercial	JJ
cord-347472-n6811ens	281	5	RNA	RNA	NNP
cord-347472-n6811ens	281	6	or	or	CC
cord-347472-n6811ens	281	7	DNA	DNA	NNP
cord-347472-n6811ens	281	8	were	be	VBD
cord-347472-n6811ens	281	9	used	use	VBN
cord-347472-n6811ens	281	10	as	as	IN
cord-347472-n6811ens	281	11	input	input	NN
cord-347472-n6811ens	281	12	for	for	IN
cord-347472-n6811ens	281	13	reactions	reaction	NNS
cord-347472-n6811ens	281	14	containing	contain	VBG
cord-347472-n6811ens	281	15	either	either	CC
cord-347472-n6811ens	281	16	Viral	Viral	NNP
cord-347472-n6811ens	281	17	One	one	CD
cord-347472-n6811ens	281	18	-	-	HYPH
cord-347472-n6811ens	281	19	step	step	NN
cord-347472-n6811ens	281	20	RT	RT	NNP
cord-347472-n6811ens	281	21	-	-	HYPH
cord-347472-n6811ens	281	22	qPCR	qPCR	NNP
cord-347472-n6811ens	281	23	mastermix	mastermix	NNP
cord-347472-n6811ens	281	24	(	(	-LRB-
cord-347472-n6811ens	281	25	containing	contain	VBG
cord-347472-n6811ens	281	26	reverse	reverse	JJ
cord-347472-n6811ens	281	27	transcriptase	transcriptase	NN
cord-347472-n6811ens	281	28	)	)	-RRB-
cord-347472-n6811ens	281	29	or	or	CC
cord-347472-n6811ens	281	30	TaqMan	TaqMan	NNP
cord-347472-n6811ens	281	31	Fast	Fast	NNP
cord-347472-n6811ens	281	32	qPCR	qPCR	NNP
cord-347472-n6811ens	281	33	mastermix	mastermix	FW
cord-347472-n6811ens	281	34	(	(	-LRB-
cord-347472-n6811ens	281	35	lacking	lack	VBG
cord-347472-n6811ens	281	36	reverse	reverse	JJ
cord-347472-n6811ens	281	37	transcriptase	transcriptase	NN
cord-347472-n6811ens	281	38	)	)	-RRB-
cord-347472-n6811ens	281	39	using	use	VBG
cord-347472-n6811ens	281	40	either	either	CC
cord-347472-n6811ens	281	41	the	the	DT
cord-347472-n6811ens	281	42	CDC	CDC	NNP
cord-347472-n6811ens	281	43	-	-	HYPH
cord-347472-n6811ens	281	44	specified	specify	VBN
cord-347472-n6811ens	281	45	or	or	CC
cord-347472-n6811ens	281	46	transcript	transcript	NN
cord-347472-n6811ens	281	47	-	-	HYPH
cord-347472-n6811ens	281	48	specific	specific	JJ
cord-347472-n6811ens	281	49	RPP30	RPP30	NNP
cord-347472-n6811ens	281	50	probe	probe	NN
cord-347472-n6811ens	281	51	.	.	.
cord-347472-n6811ens	282	1	The	the	DT
cord-347472-n6811ens	282	2	transcript	transcript	NN
cord-347472-n6811ens	282	3	-	-	HYPH
cord-347472-n6811ens	282	4	specific	specific	JJ
cord-347472-n6811ens	282	5	probe	probe	NN
cord-347472-n6811ens	282	6	generates	generate	VBZ
cord-347472-n6811ens	282	7	signal	signal	NN
cord-347472-n6811ens	282	8	only	only	RB
cord-347472-n6811ens	282	9	in	in	IN
cord-347472-n6811ens	282	10	reactions	reaction	NNS
cord-347472-n6811ens	282	11	with	with	IN
cord-347472-n6811ens	282	12	an	an	DT
cord-347472-n6811ens	282	13	RNA	RNA	NNP
cord-347472-n6811ens	282	14	input	input	NN
cord-347472-n6811ens	282	15	where	where	WRB
cord-347472-n6811ens	282	16	reverse	reverse	NN
cord-347472-n6811ens	282	17	-	-	HYPH
cord-347472-n6811ens	282	18	transcriptase	transcriptase	NN
cord-347472-n6811ens	282	19	is	be	VBZ
cord-347472-n6811ens	282	20	present	present	JJ
cord-347472-n6811ens	282	21	(	(	-LRB-
cord-347472-n6811ens	282	22	as	as	IN
cord-347472-n6811ens	282	23	a	a	DT
cord-347472-n6811ens	282	24	one	one	CD
cord-347472-n6811ens	282	25	-	-	HYPH
cord-347472-n6811ens	282	26	step	step	NN
cord-347472-n6811ens	282	27	mastermix	mastermix	NN
cord-347472-n6811ens	282	28	)	)	-RRB-
cord-347472-n6811ens	282	29	.	.	.
cord-347472-n6811ens	283	1	Genomic	genomic	JJ
cord-347472-n6811ens	283	2	DNA	dna	NN
cord-347472-n6811ens	283	3	does	do	VBZ
cord-347472-n6811ens	283	4	not	not	RB
cord-347472-n6811ens	283	5	generate	generate	VB
cord-347472-n6811ens	283	6	a	a	DT
cord-347472-n6811ens	283	7	signal	signal	NN
cord-347472-n6811ens	283	8	above	above	IN
cord-347472-n6811ens	283	9	baseline	baseline	NN
cord-347472-n6811ens	283	10	even	even	RB
cord-347472-n6811ens	283	11	in	in	IN
cord-347472-n6811ens	283	12	an	an	DT
cord-347472-n6811ens	283	13	extended	extended	JJ
cord-347472-n6811ens	283	14	50-cycle	50-cycle	CD
cord-347472-n6811ens	283	15	amplification	amplification	NN
cord-347472-n6811ens	283	16	,	,	,
cord-347472-n6811ens	283	17	nor	nor	CC
cord-347472-n6811ens	283	18	does	do	VBZ
cord-347472-n6811ens	283	19	RNA	RNA	NNP
cord-347472-n6811ens	283	20	generate	generate	VB
cord-347472-n6811ens	283	21	a	a	DT
cord-347472-n6811ens	283	22	signal	signal	NN
cord-347472-n6811ens	283	23	in	in	IN
cord-347472-n6811ens	283	24	a	a	DT
cord-347472-n6811ens	283	25	qPCR	qpcr	RB
cord-347472-n6811ens	283	26	-	-	HYPH
cord-347472-n6811ens	283	27	only	only	RB
cord-347472-n6811ens	283	28	(	(	-LRB-
cord-347472-n6811ens	283	29	no	no	DT
cord-347472-n6811ens	283	30	reverse	reverse	JJ
cord-347472-n6811ens	283	31	transcriptase	transcriptase	NN
cord-347472-n6811ens	283	32	)	)	-RRB-
cord-347472-n6811ens	283	33	reaction	reaction	NN
cord-347472-n6811ens	283	34	for	for	IN
cord-347472-n6811ens	283	35	a	a	DT
cord-347472-n6811ens	283	36	transcriptspecific	transcriptspecific	NNP
cord-347472-n6811ens	283	37	probe	probe	NN
cord-347472-n6811ens	283	38	.	.	.
cord-347472-n6811ens	284	1	Signal	signal	NN
cord-347472-n6811ens	284	2	generated	generate	VBN
cord-347472-n6811ens	284	3	for	for	IN
cord-347472-n6811ens	284	4	CDC	CDC	NNP
cord-347472-n6811ens	284	5	-	-	HYPH
cord-347472-n6811ens	284	6	RP	RP	NNP
cord-347472-n6811ens	284	7	probe	probe	NN
cord-347472-n6811ens	284	8	in	in	IN
cord-347472-n6811ens	284	9	PCR	PCR	NNP
cord-347472-n6811ens	284	10	-	-	HYPH
cord-347472-n6811ens	284	11	only	only	RB
cord-347472-n6811ens	284	12	analysis	analysis	NN
cord-347472-n6811ens	284	13	of	of	IN
cord-347472-n6811ens	284	14	"	"	``
cord-347472-n6811ens	284	15	RNA	RNA	NNP
cord-347472-n6811ens	284	16	"	"	''
cord-347472-n6811ens	284	17	is	be	VBZ
cord-347472-n6811ens	284	18	due	due	JJ
cord-347472-n6811ens	284	19	to	to	IN
cord-347472-n6811ens	284	20	low	low	JJ
cord-347472-n6811ens	284	21	-	-	HYPH
cord-347472-n6811ens	284	22	concentration	concentration	NN
cord-347472-n6811ens	284	23	genomic	genomic	JJ
cord-347472-n6811ens	284	24	DNA	dna	NN
cord-347472-n6811ens	284	25	present	present	NN
cord-347472-n6811ens	284	26	in	in	IN
cord-347472-n6811ens	284	27	Quantigene	Quantigene	NNP
cord-347472-n6811ens	284	28	commercial	commercial	NN
cord-347472-n6811ens	284	29	RNA	RNA	NNP
cord-347472-n6811ens	284	30	(	(	-LRB-
cord-347472-n6811ens	284	31	supplemental	supplemental	JJ
cord-347472-n6811ens	284	32	discussion	discussion	NN
cord-347472-n6811ens	284	33	)	)	-RRB-
cord-347472-n6811ens	284	34	,	,	,
cord-347472-n6811ens	284	35	further	further	RB
cord-347472-n6811ens	284	36	underscoring	underscore	VBG
cord-347472-n6811ens	284	37	the	the	DT
cord-347472-n6811ens	284	38	need	need	NN
cord-347472-n6811ens	284	39	for	for	IN
cord-347472-n6811ens	284	40	transcript	transcript	NN
cord-347472-n6811ens	284	41	-	-	HYPH
cord-347472-n6811ens	284	42	specific	specific	JJ
cord-347472-n6811ens	284	43	primer	primer	NN
cord-347472-n6811ens	284	44	design	design	NN
cord-347472-n6811ens	284	45	.	.	.
cord-347472-n6811ens	285	1	The	the	DT
cord-347472-n6811ens	285	2	cDNA	cdna	NN
cord-347472-n6811ens	285	3	-	-	HYPH
cord-347472-n6811ens	285	4	specific	specific	JJ
cord-347472-n6811ens	285	5	RPP30	rpp30	IN
cord-347472-n6811ens	285	6	assay	assay	NN
cord-347472-n6811ens	285	7	used	use	VBN
cord-347472-n6811ens	285	8	here	here	RB
cord-347472-n6811ens	285	9	is	be	VBZ
cord-347472-n6811ens	285	10	doubly	doubly	RB
cord-347472-n6811ens	285	11	-	-	HYPH
cord-347472-n6811ens	285	12	unable	unable	JJ
cord-347472-n6811ens	285	13	to	to	TO
cord-347472-n6811ens	285	14	generate	generate	VB
cord-347472-n6811ens	285	15	signal	signal	NN
cord-347472-n6811ens	285	16	qPCR	qpcr	NN
cord-347472-n6811ens	285	17	signals	signal	NNS
cord-347472-n6811ens	285	18	from	from	IN
cord-347472-n6811ens	285	19	genomic	genomic	NN
cord-347472-n6811ens	285	20	-	-	HYPH
cord-347472-n6811ens	285	21	DNA	dna	NN
cord-347472-n6811ens	285	22	.	.	.
cord-347472-n6811ens	286	1	Reverse	reverse	VB
cord-347472-n6811ens	286	2	-	-	HYPH
cord-347472-n6811ens	286	3	transcribed	transcribe	VBN
cord-347472-n6811ens	286	4	RPP30	RPP30	NNP
cord-347472-n6811ens	286	5	RNA	RNA	NNP
cord-347472-n6811ens	286	6	can	can	MD
cord-347472-n6811ens	286	7	serve	serve	VB
cord-347472-n6811ens	286	8	as	as	IN
cord-347472-n6811ens	286	9	a	a	DT
cord-347472-n6811ens	286	10	template	template	NN
cord-347472-n6811ens	286	11	for	for	IN
cord-347472-n6811ens	286	12	the	the	DT
cord-347472-n6811ens	286	13	Hs	Hs	NNP
cord-347472-n6811ens	286	14	.	.	.
cord-347472-n6811ens	287	1	PT.58.2854947	PT.58.2854947	NNP
cord-347472-n6811ens	287	2	qPCR	qPCR	NNP
cord-347472-n6811ens	287	3	assay	assay	NNP
cord-347472-n6811ens	287	4	generating	generate	VBG
cord-347472-n6811ens	287	5	a	a	DT
cord-347472-n6811ens	287	6	relatively	relatively	RB
cord-347472-n6811ens	287	7	short	short	JJ
cord-347472-n6811ens	287	8	amplicon	amplicon	NN
cord-347472-n6811ens	287	9	(	(	-LRB-
cord-347472-n6811ens	287	10	S3a	s3a	NN
cord-347472-n6811ens	287	11	)	)	-RRB-
cord-347472-n6811ens	287	12	.	.	.
cord-347472-n6811ens	288	1	The	the	DT
cord-347472-n6811ens	288	2	forward	forward	NN
cord-347472-n6811ens	288	3	and	and	CC
cord-347472-n6811ens	288	4	reverse	reverse	VB
cord-347472-n6811ens	288	5	primers	primer	NNS
cord-347472-n6811ens	288	6	bind	bind	VBP
cord-347472-n6811ens	288	7	in	in	IN
cord-347472-n6811ens	288	8	the	the	DT
cord-347472-n6811ens	288	9	bodies	body	NNS
cord-347472-n6811ens	288	10	of	of	IN
cord-347472-n6811ens	288	11	exon	exon	NN
cord-347472-n6811ens	288	12	1	1	CD
cord-347472-n6811ens	288	13	and	and	CC
cord-347472-n6811ens	288	14	3	3	CD
cord-347472-n6811ens	288	15	,	,	,
cord-347472-n6811ens	288	16	respectively	respectively	RB
cord-347472-n6811ens	288	17	.	.	.
cord-347472-n6811ens	289	1	The	the	DT
cord-347472-n6811ens	289	2	hydrolysis	hydrolysis	NN
cord-347472-n6811ens	289	3	probe	probe	NN
cord-347472-n6811ens	289	4	binds	bind	VBZ
cord-347472-n6811ens	289	5	at	at	IN
cord-347472-n6811ens	289	6	the	the	DT
cord-347472-n6811ens	289	7	exon	exon	NN
cord-347472-n6811ens	289	8	1	1	CD
cord-347472-n6811ens	289	9	-exon	-exon	SYM
cord-347472-n6811ens	289	10	3	3	CD
cord-347472-n6811ens	289	11	junction	junction	NN
cord-347472-n6811ens	289	12	that	that	WDT
cord-347472-n6811ens	289	13	is	be	VBZ
cord-347472-n6811ens	289	14	formed	form	VBN
cord-347472-n6811ens	289	15	during	during	IN
cord-347472-n6811ens	289	16	mRNA	mRNA	NNP
cord-347472-n6811ens	289	17	splicing	splicing	NN
cord-347472-n6811ens	289	18	.	.	.
cord-347472-n6811ens	290	1	This	this	DT
cord-347472-n6811ens	290	2	primer	primer	NN
cord-347472-n6811ens	290	3	/	/	SYM
cord-347472-n6811ens	290	4	probe	probe	NN
cord-347472-n6811ens	290	5	set	set	NN
cord-347472-n6811ens	290	6	does	do	VBZ
cord-347472-n6811ens	290	7	not	not	RB
cord-347472-n6811ens	290	8	generate	generate	VB
cord-347472-n6811ens	290	9	a	a	DT
cord-347472-n6811ens	290	10	signal	signal	NN
cord-347472-n6811ens	290	11	from	from	IN
cord-347472-n6811ens	290	12	a	a	DT
cord-347472-n6811ens	290	13	genomic	genomic	JJ
cord-347472-n6811ens	290	14	DNA	dna	NN
cord-347472-n6811ens	290	15	template	template	NN
cord-347472-n6811ens	290	16	(	(	-LRB-
cord-347472-n6811ens	290	17	shown	show	VBN
cord-347472-n6811ens	290	18	above	above	RB
cord-347472-n6811ens	290	19	)	)	-RRB-
cord-347472-n6811ens	290	20	,	,	,
cord-347472-n6811ens	290	21	due	due	IN
cord-347472-n6811ens	290	22	to	to	IN
cord-347472-n6811ens	290	23	the	the	DT
cord-347472-n6811ens	290	24	~3.5	~3.5	CD
cord-347472-n6811ens	290	25	kb	kb	NNP
cord-347472-n6811ens	290	26	spacing	space	VBG
cord-347472-n6811ens	290	27	between	between	IN
cord-347472-n6811ens	290	28	the	the	DT
cord-347472-n6811ens	290	29	forward	forward	NN
cord-347472-n6811ens	290	30	and	and	CC
cord-347472-n6811ens	290	31	reverse	reverse	VB
cord-347472-n6811ens	290	32	primers	primer	NNS
cord-347472-n6811ens	290	33	that	that	WDT
cord-347472-n6811ens	290	34	is	be	VBZ
cord-347472-n6811ens	290	35	un	un	NNP
cord-347472-n6811ens	290	36	-	-	JJ
cord-347472-n6811ens	290	37	amplifiable	amplifiable	JJ
cord-347472-n6811ens	290	38	using	use	VBG
cord-347472-n6811ens	290	39	the	the	DT
cord-347472-n6811ens	290	40	short	short	JJ
cord-347472-n6811ens	290	41	annealing	annealing	NN
cord-347472-n6811ens	290	42	/	/	SYM
cord-347472-n6811ens	290	43	extension	extension	NN
cord-347472-n6811ens	290	44	cycles	cycle	NNS
cord-347472-n6811ens	290	45	of	of	IN
cord-347472-n6811ens	290	46	the	the	DT
cord-347472-n6811ens	290	47	CDC	CDC	NNP
cord-347472-n6811ens	290	48	ramping	ramp	VBG
cord-347472-n6811ens	290	49	program	program	NN
cord-347472-n6811ens	290	50	(	(	-LRB-
cord-347472-n6811ens	290	51	S3b	s3b	NN
cord-347472-n6811ens	290	52	)	)	-RRB-
cord-347472-n6811ens	290	53	.	.	.
cord-347472-n6811ens	291	1	Even	even	RB
cord-347472-n6811ens	291	2	if	if	IN
cord-347472-n6811ens	291	3	PCR	PCR	NNP
cord-347472-n6811ens	291	4	product	product	NN
cord-347472-n6811ens	291	5	were	be	VBD
cord-347472-n6811ens	291	6	generated	generate	VBN
cord-347472-n6811ens	291	7	,	,	,
cord-347472-n6811ens	291	8	the	the	DT
cord-347472-n6811ens	291	9	hydrolysis	hydrolysis	NN
cord-347472-n6811ens	291	10	probe	probe	NN
cord-347472-n6811ens	291	11	binding	bind	VBG
cord-347472-n6811ens	291	12	sequence	sequence	NN
cord-347472-n6811ens	291	13	is	be	VBZ
cord-347472-n6811ens	291	14	split	split	VBN
cord-347472-n6811ens	291	15	in	in	IN
cord-347472-n6811ens	291	16	half	half	NN
cord-347472-n6811ens	291	17	,	,	,
cord-347472-n6811ens	291	18	with	with	IN
cord-347472-n6811ens	291	19	each	each	DT
cord-347472-n6811ens	291	20	side	side	NN
cord-347472-n6811ens	291	21	~3	~3	-RRB-
cord-347472-n6811ens	291	22	kb	kb	XX
cord-347472-n6811ens	291	23	apart	apart	RB
cord-347472-n6811ens	291	24	in	in	IN
cord-347472-n6811ens	291	25	a	a	DT
cord-347472-n6811ens	291	26	genome	genome	NN
cord-347472-n6811ens	291	27	-	-	HYPH
cord-347472-n6811ens	291	28	templated	templated	JJ
cord-347472-n6811ens	291	29	amplicon	amplicon	NN
cord-347472-n6811ens	291	30	.	.	.
cord-347472-n6811ens	292	1	Colleagues	colleague	NNS
cord-347472-n6811ens	292	2	at	at	IN
cord-347472-n6811ens	292	3	the	the	DT
cord-347472-n6811ens	292	4	CDC	CDC	NNP
cord-347472-n6811ens	292	5	,	,	,
cord-347472-n6811ens	292	6	I	-PRON-	PRP
cord-347472-n6811ens	292	7	am	be	VBP
cord-347472-n6811ens	292	8	writing	write	VBG
cord-347472-n6811ens	292	9	to	to	TO
cord-347472-n6811ens	292	10	express	express	VB
cord-347472-n6811ens	292	11	my	-PRON-	PRP$
cord-347472-n6811ens	292	12	concern	concern	NN
cord-347472-n6811ens	292	13	that	that	IN
cord-347472-n6811ens	292	14	the	the	DT
cord-347472-n6811ens	292	15	RNase	RNase	NNP
cord-347472-n6811ens	292	16	P	p	NN
cord-347472-n6811ens	292	17	probe	probe	NN
cord-347472-n6811ens	292	18	sequence	sequence	NN
cord-347472-n6811ens	292	19	specified	specify	VBN
cord-347472-n6811ens	292	20	in	in	IN
cord-347472-n6811ens	292	21	the	the	DT
cord-347472-n6811ens	292	22	2019-nCoVEUA-01	2019-ncoveua-01	CD
cord-347472-n6811ens	292	23	Revision	revision	NN
cord-347472-n6811ens	292	24	2	2	CD
cord-347472-n6811ens	292	25	assay	assay	NN
cord-347472-n6811ens	292	26	does	do	VBZ
cord-347472-n6811ens	292	27	not	not	RB
cord-347472-n6811ens	292	28	serve	serve	VB
cord-347472-n6811ens	292	29	its	-PRON-	PRP$
cord-347472-n6811ens	292	30	desired	desire	VBN
cord-347472-n6811ens	292	31	function	function	NN
cord-347472-n6811ens	292	32	as	as	IN
cord-347472-n6811ens	292	33	a	a	DT
cord-347472-n6811ens	292	34	control	control	NN
cord-347472-n6811ens	292	35	of	of	IN
cord-347472-n6811ens	292	36	sample	sample	NN
cord-347472-n6811ens	292	37	collection	collection	NN
cord-347472-n6811ens	292	38	and	and	CC
cord-347472-n6811ens	292	39	reverse	reverse	VB
cord-347472-n6811ens	292	40	transcription	transcription	NN
cord-347472-n6811ens	292	41	.	.	.
cord-347472-n6811ens	293	1	The	the	DT
cord-347472-n6811ens	293	2	goal	goal	NN
cord-347472-n6811ens	293	3	of	of	IN
cord-347472-n6811ens	293	4	the	the	DT
cord-347472-n6811ens	293	5	RNaseP	RNaseP	NNP
cord-347472-n6811ens	293	6	probe	probe	NN
cord-347472-n6811ens	293	7	is	be	VBZ
cord-347472-n6811ens	293	8	to	to	TO
cord-347472-n6811ens	293	9	provide	provide	VB
cord-347472-n6811ens	293	10	a	a	DT
cord-347472-n6811ens	293	11	composite	composite	JJ
cord-347472-n6811ens	293	12	control	control	NN
cord-347472-n6811ens	293	13	that	that	WDT
cord-347472-n6811ens	293	14	is	be	VBZ
cord-347472-n6811ens	293	15	positive	positive	JJ
cord-347472-n6811ens	293	16	if	if	IN
cord-347472-n6811ens	293	17	sufficient	sufficient	JJ
cord-347472-n6811ens	293	18	specimen	specimen	NN
cord-347472-n6811ens	293	19	,	,	,
cord-347472-n6811ens	293	20	including	include	VBG
cord-347472-n6811ens	293	21	intact	intact	JJ
cord-347472-n6811ens	293	22	RNA	RNA	NNP
cord-347472-n6811ens	293	23	,	,	,
cord-347472-n6811ens	293	24	has	have	VBZ
cord-347472-n6811ens	293	25	been	be	VBN
cord-347472-n6811ens	293	26	collected	collect	VBN
cord-347472-n6811ens	293	27	AND	and	CC
cord-347472-n6811ens	293	28	a	a	DT
cord-347472-n6811ens	293	29	successful	successful	JJ
cord-347472-n6811ens	293	30	reverse	reverse	NN
cord-347472-n6811ens	293	31	-	-	HYPH
cord-347472-n6811ens	293	32	transcription	transcription	NN
cord-347472-n6811ens	293	33	qPCR	qpcr	NN
cord-347472-n6811ens	293	34	has	have	VBZ
cord-347472-n6811ens	293	35	occurred	occur	VBN
cord-347472-n6811ens	293	36	.	.	.
cord-347472-n6811ens	294	1	As	as	IN
cord-347472-n6811ens	294	2	per	per	IN
cord-347472-n6811ens	294	3	the	the	DT
cord-347472-n6811ens	294	4	EUA	EUA	NNP
cord-347472-n6811ens	294	5	,	,	,
cord-347472-n6811ens	294	6	"	"	``
cord-347472-n6811ens	294	7	Failure	failure	NN
cord-347472-n6811ens	294	8	to	to	TO
cord-347472-n6811ens	294	9	detect	detect	VB
cord-347472-n6811ens	294	10	RNase	RNase	NNP
cord-347472-n6811ens	294	11	P	p	NN
cord-347472-n6811ens	294	12	in	in	IN
cord-347472-n6811ens	294	13	any	any	DT
cord-347472-n6811ens	294	14	clinical	clinical	JJ
cord-347472-n6811ens	294	15	specimens	specimen	NNS
cord-347472-n6811ens	294	16	may	may	MD
cord-347472-n6811ens	294	17	indicate	indicate	VB
cord-347472-n6811ens	294	18	:	:	:
cord-347472-n6811ens	294	19	−	−	NNS
cord-347472-n6811ens	294	20	Improper	improper	JJ
cord-347472-n6811ens	294	21	extraction	extraction	NN
cord-347472-n6811ens	294	22	of	of	IN
cord-347472-n6811ens	294	23	nucleic	nucleic	JJ
cord-347472-n6811ens	294	24	acid	acid	NN
cord-347472-n6811ens	294	25	from	from	IN
cord-347472-n6811ens	294	26	clinical	clinical	JJ
cord-347472-n6811ens	294	27	materials	material	NNS
cord-347472-n6811ens	294	28	resulting	result	VBG
cord-347472-n6811ens	294	29	in	in	IN
cord-347472-n6811ens	294	30	loss	loss	NN
cord-347472-n6811ens	294	31	of	of	IN
cord-347472-n6811ens	294	32	RNA	RNA	NNP
cord-347472-n6811ens	294	33	and/or	and/or	CC
cord-347472-n6811ens	294	34	RNA	RNA	NNP
cord-347472-n6811ens	294	35	degradation	degradation	NN
cord-347472-n6811ens	294	36	.	.	.
cord-347472-n6811ens	295	1	"	"	''
cord-347472-n6811ens	296	1	The	the	DT
cord-347472-n6811ens	296	2	specified	specified	JJ
cord-347472-n6811ens	296	3	RNaseP	rnasep	NN
cord-347472-n6811ens	296	4	probe	probe	NN
cord-347472-n6811ens	296	5	is	be	VBZ
cord-347472-n6811ens	296	6	not	not	RB
cord-347472-n6811ens	296	7	selective	selective	JJ
cord-347472-n6811ens	296	8	for	for	IN
cord-347472-n6811ens	296	9	the	the	DT
cord-347472-n6811ens	296	10	RNaseP	RNaseP	NNP
cord-347472-n6811ens	296	11	transcript	transcript	NN
cord-347472-n6811ens	296	12	.	.	.
cord-347472-n6811ens	297	1	The	the	DT
cord-347472-n6811ens	297	2	amplicon	amplicon	NN
cord-347472-n6811ens	297	3	and	and	CC
cord-347472-n6811ens	297	4	probe	probe	NN
cord-347472-n6811ens	297	5	sequence	sequence	NN
cord-347472-n6811ens	297	6	are	be	VBP
cord-347472-n6811ens	297	7	contained	contain	VBN
cord-347472-n6811ens	297	8	within	within	IN
cord-347472-n6811ens	297	9	a	a	DT
cord-347472-n6811ens	297	10	single	single	JJ
cord-347472-n6811ens	297	11	exon	exon	NN
cord-347472-n6811ens	297	12	,	,	,
cord-347472-n6811ens	297	13	do	do	VBP
cord-347472-n6811ens	297	14	not	not	RB
cord-347472-n6811ens	297	15	span	span	VB
cord-347472-n6811ens	297	16	an	an	DT
cord-347472-n6811ens	297	17	exon	exon	NN
cord-347472-n6811ens	297	18	-	-	HYPH
cord-347472-n6811ens	297	19	exon	exon	JJ
cord-347472-n6811ens	297	20	junction	junction	NN
cord-347472-n6811ens	297	21	,	,	,
cord-347472-n6811ens	297	22	and	and	CC
cord-347472-n6811ens	297	23	therefore	therefore	RB
cord-347472-n6811ens	297	24	detect	detect	VBP
cord-347472-n6811ens	297	25	both	both	DT
cord-347472-n6811ens	297	26	RNA	RNA	NNP
cord-347472-n6811ens	297	27	and	and	CC
cord-347472-n6811ens	297	28	DNA	dna	NN
cord-347472-n6811ens	297	29	templates	template	NNS
cord-347472-n6811ens	297	30	(	(	-LRB-
cord-347472-n6811ens	297	31	see	see	VB
cord-347472-n6811ens	297	32	illustration	illustration	NN
cord-347472-n6811ens	297	33	on	on	IN
cord-347472-n6811ens	297	34	following	follow	VBG
cord-347472-n6811ens	297	35	page	page	NN
cord-347472-n6811ens	297	36	)	)	-RRB-
cord-347472-n6811ens	297	37	.	.	.
cord-347472-n6811ens	298	1	This	this	DT
cord-347472-n6811ens	298	2	fundamental	fundamental	JJ
cord-347472-n6811ens	298	3	problem	problem	NN
cord-347472-n6811ens	298	4	undermines	undermine	VBZ
cord-347472-n6811ens	298	5	the	the	DT
cord-347472-n6811ens	298	6	control	control	NN
cord-347472-n6811ens	298	7	and	and	CC
cord-347472-n6811ens	298	8	makes	make	VBZ
cord-347472-n6811ens	298	9	"	"	``
cord-347472-n6811ens	298	10	RNaseP	rnasep	JJ
cord-347472-n6811ens	298	11	positive	positive	JJ
cord-347472-n6811ens	298	12	,	,	,
cord-347472-n6811ens	298	13	viral	viral	JJ
cord-347472-n6811ens	298	14	transcript	transcript	NN
cord-347472-n6811ens	298	15	negative	negative	JJ
cord-347472-n6811ens	298	16	"	"	``
cord-347472-n6811ens	298	17	samples	sample	NNS
cord-347472-n6811ens	298	18	ambiguous	ambiguous	JJ
cord-347472-n6811ens	298	19	.	.	.
cord-347472-n6811ens	299	1	Samples	sample	NNS
cord-347472-n6811ens	299	2	with	with	IN
cord-347472-n6811ens	299	3	degraded	degrade	VBN
cord-347472-n6811ens	299	4	RNA	RNA	NNP
cord-347472-n6811ens	299	5	(	(	-LRB-
cord-347472-n6811ens	299	6	but	but	CC
cord-347472-n6811ens	299	7	intact	intact	JJ
cord-347472-n6811ens	299	8	human	human	JJ
cord-347472-n6811ens	299	9	DNA	dna	NN
cord-347472-n6811ens	299	10	)	)	-RRB-
cord-347472-n6811ens	299	11	will	will	MD
cord-347472-n6811ens	299	12	generate	generate	VB
cord-347472-n6811ens	299	13	a	a	DT
cord-347472-n6811ens	299	14	qPCR	qpcr	JJ
cord-347472-n6811ens	299	15	signal	signal	NN
cord-347472-n6811ens	299	16	for	for	IN
cord-347472-n6811ens	299	17	the	the	DT
cord-347472-n6811ens	299	18	RNaseP	RNaseP	NNP
cord-347472-n6811ens	299	19	control	control	NN
cord-347472-n6811ens	299	20	due	due	JJ
cord-347472-n6811ens	299	21	to	to	IN
cord-347472-n6811ens	299	22	contaminating	contaminate	VBG
cord-347472-n6811ens	299	23	DNA	dna	NN
cord-347472-n6811ens	299	24	,	,	,
cord-347472-n6811ens	299	25	but	but	CC
cord-347472-n6811ens	299	26	return	return	VB
cord-347472-n6811ens	299	27	a	a	DT
cord-347472-n6811ens	299	28	false	false	JJ
cord-347472-n6811ens	299	29	negative	negative	NN
cord-347472-n6811ens	299	30	for	for	IN
cord-347472-n6811ens	299	31	N1	N1	NNP
cord-347472-n6811ens	299	32	and	and	CC
cord-347472-n6811ens	299	33	N2	N2	NNP
cord-347472-n6811ens	299	34	(	(	-LRB-
cord-347472-n6811ens	299	35	viral	viral	JJ
cord-347472-n6811ens	299	36	)	)	-RRB-
cord-347472-n6811ens	299	37	amplicons	amplicon	NNS
cord-347472-n6811ens	299	38	due	due	JJ
cord-347472-n6811ens	299	39	to	to	IN
cord-347472-n6811ens	299	40	the	the	DT
cord-347472-n6811ens	299	41	absence	absence	NN
cord-347472-n6811ens	299	42	of	of	IN
cord-347472-n6811ens	299	43	intact	intact	JJ
cord-347472-n6811ens	299	44	RNA	RNA	NNP
cord-347472-n6811ens	299	45	.	.	.
cord-347472-n6811ens	300	1	Similarly	similarly	RB
cord-347472-n6811ens	300	2	,	,	,
cord-347472-n6811ens	300	3	failure	failure	NN
cord-347472-n6811ens	300	4	of	of	IN
cord-347472-n6811ens	300	5	the	the	DT
cord-347472-n6811ens	300	6	reverse	reverse	JJ
cord-347472-n6811ens	300	7	transcription	transcription	NN
cord-347472-n6811ens	300	8	step	step	NN
cord-347472-n6811ens	300	9	precludes	preclude	VBZ
cord-347472-n6811ens	300	10	viral	viral	JJ
cord-347472-n6811ens	300	11	cDNA	cdna	NN
cord-347472-n6811ens	300	12	synthesis	synthesis	NN
cord-347472-n6811ens	300	13	,	,	,
cord-347472-n6811ens	300	14	but	but	CC
cord-347472-n6811ens	300	15	any	any	DT
cord-347472-n6811ens	300	16	human	human	JJ
cord-347472-n6811ens	300	17	genomic	genomic	JJ
cord-347472-n6811ens	300	18	DNA	dna	NN
cord-347472-n6811ens	300	19	will	will	MD
cord-347472-n6811ens	300	20	generate	generate	VB
cord-347472-n6811ens	300	21	a	a	DT
cord-347472-n6811ens	300	22	positive	positive	JJ
cord-347472-n6811ens	300	23	RP	rp	NN
cord-347472-n6811ens	300	24	control	control	NN
cord-347472-n6811ens	300	25	signal	signal	NN
cord-347472-n6811ens	300	26	.	.	.
cord-347472-n6811ens	301	1	Although	although	IN
cord-347472-n6811ens	301	2	the	the	DT
cord-347472-n6811ens	301	3	RP	rp	NN
cord-347472-n6811ens	301	4	sequences	sequence	NNS
cord-347472-n6811ens	301	5	are	be	VBP
cord-347472-n6811ens	301	6	inherent	inherent	JJ
cord-347472-n6811ens	301	7	non	non	JJ
cord-347472-n6811ens	301	8	-	-	JJ
cord-347472-n6811ens	301	9	selective	selective	JJ
cord-347472-n6811ens	301	10	for	for	IN
cord-347472-n6811ens	301	11	RNaseP	RNaseP	NNP
cord-347472-n6811ens	301	12	RNA	RNA	NNP
cord-347472-n6811ens	301	13	,	,	,
cord-347472-n6811ens	301	14	it	-PRON-	PRP
cord-347472-n6811ens	301	15	is	be	VBZ
cord-347472-n6811ens	301	16	conceivable	conceivable	JJ
cord-347472-n6811ens	301	17	that	that	IN
cord-347472-n6811ens	301	18	this	this	DT
cord-347472-n6811ens	301	19	primer	primer	NN
cord-347472-n6811ens	301	20	was	be	VBD
cord-347472-n6811ens	301	21	functionally	functionally	RB
cord-347472-n6811ens	301	22	tested	test	VBN
cord-347472-n6811ens	301	23	in	in	IN
cord-347472-n6811ens	301	24	ideal	ideal	JJ
cord-347472-n6811ens	301	25	conditions	condition	NNS
cord-347472-n6811ens	301	26	where	where	WRB
cord-347472-n6811ens	301	27	sample	sample	NN
cord-347472-n6811ens	301	28	extraction	extraction	NN
cord-347472-n6811ens	301	29	generated	generate	VBD
cord-347472-n6811ens	301	30	pure	pure	NNP
cord-347472-n6811ens	301	31	RNA	RNA	NNP
cord-347472-n6811ens	301	32	.	.	.
cord-347472-n6811ens	302	1	As	as	IN
cord-347472-n6811ens	302	2	the	the	DT
cord-347472-n6811ens	302	3	range	range	NN
cord-347472-n6811ens	302	4	of	of	IN
cord-347472-n6811ens	302	5	sample	sample	NN
cord-347472-n6811ens	302	6	preparation	preparation	NN
cord-347472-n6811ens	302	7	protocols	protocol	NNS
cord-347472-n6811ens	302	8	used	use	VBN
cord-347472-n6811ens	302	9	expands	expand	VBZ
cord-347472-n6811ens	302	10	(	(	-LRB-
cord-347472-n6811ens	302	11	either	either	CC
cord-347472-n6811ens	302	12	as	as	IN
cord-347472-n6811ens	302	13	specified	specify	VBN
cord-347472-n6811ens	302	14	in	in	IN
cord-347472-n6811ens	302	15	the	the	DT
cord-347472-n6811ens	302	16	EUA	EUA	NNP
cord-347472-n6811ens	302	17	or	or	CC
cord-347472-n6811ens	302	18	off	off	IN
cord-347472-n6811ens	302	19	-	-	HYPH
cord-347472-n6811ens	302	20	label	label	NN
cord-347472-n6811ens	302	21	)	)	-RRB-
cord-347472-n6811ens	302	22	,	,	,
cord-347472-n6811ens	302	23	DNA	dna	NN
cord-347472-n6811ens	302	24	purification	purification	NN
cord-347472-n6811ens	302	25	alongside	alongside	IN
cord-347472-n6811ens	302	26	RNA	RNA	NNP
cord-347472-n6811ens	302	27	is	be	VBZ
cord-347472-n6811ens	302	28	inevitable	inevitable	JJ
cord-347472-n6811ens	302	29	.	.	.
cord-347472-n6811ens	303	1	As	as	IN
cord-347472-n6811ens	303	2	presently	presently	RB
cord-347472-n6811ens	303	3	designed	design	VBN
cord-347472-n6811ens	303	4	,	,	,
cord-347472-n6811ens	303	5	the	the	DT
cord-347472-n6811ens	303	6	RNaseP	RNaseP	NNP
cord-347472-n6811ens	303	7	probe	probe	NN
cord-347472-n6811ens	303	8	does	do	VBZ
cord-347472-n6811ens	303	9	not	not	RB
cord-347472-n6811ens	303	10	inform	inform	VB
cord-347472-n6811ens	303	11	about	about	IN
cord-347472-n6811ens	303	12	the	the	DT
cord-347472-n6811ens	303	13	presence	presence	NN
cord-347472-n6811ens	303	14	of	of	IN
cord-347472-n6811ens	303	15	intact	intact	JJ
cord-347472-n6811ens	303	16	specimen	specimen	NN
cord-347472-n6811ens	303	17	RNA	RNA	NNP
cord-347472-n6811ens	303	18	nor	nor	CC
cord-347472-n6811ens	303	19	does	do	VBZ
cord-347472-n6811ens	303	20	it	-PRON-	PRP
cord-347472-n6811ens	303	21	control	control	VB
cord-347472-n6811ens	303	22	for	for	IN
cord-347472-n6811ens	303	23	successful	successful	JJ
cord-347472-n6811ens	303	24	reverse	reverse	NN
cord-347472-n6811ens	303	25	transcription	transcription	NN
cord-347472-n6811ens	303	26	.	.	.
cord-347472-n6811ens	304	1	This	this	DT
cord-347472-n6811ens	304	2	has	have	VBZ
cord-347472-n6811ens	304	3	the	the	DT
cord-347472-n6811ens	304	4	potential	potential	NN
cord-347472-n6811ens	304	5	to	to	TO
cord-347472-n6811ens	304	6	adversely	adversely	RB
cord-347472-n6811ens	304	7	affect	affect	VB
cord-347472-n6811ens	304	8	clinical	clinical	JJ
cord-347472-n6811ens	304	9	decision	decision	NN
cord-347472-n6811ens	304	10	making	making	NN
cord-347472-n6811ens	304	11	.	.	.
cord-347472-n6811ens	305	1	The	the	DT
cord-347472-n6811ens	305	2	2019-nCoVEUA-01	2019-ncoveua-01	CD
cord-347472-n6811ens	305	3	Rev2	Rev2	NNP
cord-347472-n6811ens	305	4	assay	assay	NN
cord-347472-n6811ens	305	5	certainly	certainly	RB
cord-347472-n6811ens	305	6	detects	detect	VBZ
cord-347472-n6811ens	305	7	viral	viral	JJ
cord-347472-n6811ens	305	8	load	load	NN
cord-347472-n6811ens	305	9	from	from	IN
cord-347472-n6811ens	305	10	controls	control	NNS
cord-347472-n6811ens	305	11	and	and	CC
cord-347472-n6811ens	305	12	patient	patient	NN
cord-347472-n6811ens	305	13	samples	sample	NNS
cord-347472-n6811ens	305	14	(	(	-LRB-
cord-347472-n6811ens	305	15	our	-PRON-	PRP$
cord-347472-n6811ens	305	16	facility	facility	NN
cord-347472-n6811ens	305	17	has	have	VBZ
cord-347472-n6811ens	305	18	recently	recently	RB
cord-347472-n6811ens	305	19	completely	completely	RB
cord-347472-n6811ens	305	20	proficiency	proficiency	NN
cord-347472-n6811ens	305	21	testing	testing	NN
cord-347472-n6811ens	305	22	to	to	IN
cord-347472-n6811ens	305	23	that	that	DT
cord-347472-n6811ens	305	24	end	end	NN
cord-347472-n6811ens	305	25	)	)	-RRB-
cord-347472-n6811ens	305	26	,	,	,
cord-347472-n6811ens	305	27	but	but	CC
cord-347472-n6811ens	305	28	this	this	DT
cord-347472-n6811ens	305	29	assay	assay	NN
cord-347472-n6811ens	305	30	has	have	VBZ
cord-347472-n6811ens	305	31	the	the	DT
cord-347472-n6811ens	305	32	potential	potential	NN
cord-347472-n6811ens	305	33	to	to	TO
cord-347472-n6811ens	305	34	return	return	VB
cord-347472-n6811ens	305	35	false	false	JJ
cord-347472-n6811ens	305	36	negative	negative	JJ
cord-347472-n6811ens	305	37	results	result	NNS
cord-347472-n6811ens	305	38	.	.	.
cord-347472-n6811ens	306	1	I	-PRON-	PRP
cord-347472-n6811ens	306	2	suggest	suggest	VBP
cord-347472-n6811ens	306	3	that	that	IN
cord-347472-n6811ens	306	4	the	the	DT
cord-347472-n6811ens	306	5	"	"	``
cord-347472-n6811ens	306	6	2019-nCoV	2019-ncov	CD
cord-347472-n6811ens	306	7	rRT	rRT	NNP
cord-347472-n6811ens	306	8	-	-	HYPH
cord-347472-n6811ens	306	9	PCR	PCR	NNP
cord-347472-n6811ens	306	10	Diagnostic	Diagnostic	NNP
cord-347472-n6811ens	306	11	Panel	Panel	NNP
cord-347472-n6811ens	306	12	Results	Results	NNP
cord-347472-n6811ens	306	13	Interpretation	Interpretation	NNP
cord-347472-n6811ens	306	14	Guide	Guide	NNP
cord-347472-n6811ens	306	15	"	"	''
cord-347472-n6811ens	306	16	be	be	VB
cord-347472-n6811ens	306	17	updated	update	VBN
cord-347472-n6811ens	306	18	to	to	TO
cord-347472-n6811ens	306	19	reflect	reflect	VB
cord-347472-n6811ens	306	20	this	this	DT
cord-347472-n6811ens	306	21	fact	fact	NN
cord-347472-n6811ens	306	22	,	,	,
cord-347472-n6811ens	306	23	and	and	CC
cord-347472-n6811ens	306	24	that	that	IN
cord-347472-n6811ens	306	25	a	a	DT
cord-347472-n6811ens	306	26	re	re	NN
cord-347472-n6811ens	306	27	-	-	NN
cord-347472-n6811ens	306	28	design	design	NN
cord-347472-n6811ens	306	29	of	of	IN
cord-347472-n6811ens	306	30	the	the	DT
cord-347472-n6811ens	306	31	RNaseP	RNaseP	NNP
cord-347472-n6811ens	306	32	probe	probe	NN
cord-347472-n6811ens	306	33	(	(	-LRB-
cord-347472-n6811ens	306	34	to	to	TO
cord-347472-n6811ens	306	35	generate	generate	VB
cord-347472-n6811ens	306	36	a	a	DT
cord-347472-n6811ens	306	37	transcript	transcript	NN
cord-347472-n6811ens	306	38	-	-	HYPH
cord-347472-n6811ens	306	39	specific	specific	JJ
cord-347472-n6811ens	306	40	amplicon	amplicon	NN
cord-347472-n6811ens	306	41	that	that	WDT
cord-347472-n6811ens	306	42	will	will	MD
cord-347472-n6811ens	306	43	be	be	VB
cord-347472-n6811ens	306	44	insensitive	insensitive	JJ
cord-347472-n6811ens	306	45	to	to	IN
cord-347472-n6811ens	306	46	DNA	DNA	NNP
cord-347472-n6811ens	306	47	)	)	-RRB-
cord-347472-n6811ens	306	48	be	be	VB
cord-347472-n6811ens	306	49	undertaken	undertake	VBN
cord-347472-n6811ens	306	50	post	post	JJ
cord-347472-n6811ens	306	51	haste	haste	NN
cord-347472-n6811ens	306	52	.	.	.
cord-347472-n6811ens	307	1	I	-PRON-	PRP
cord-347472-n6811ens	307	2	am	be	VBP
cord-347472-n6811ens	307	3	including	include	VBG
cord-347472-n6811ens	307	4	annotations	annotation	NNS
cord-347472-n6811ens	307	5	of	of	IN
cord-347472-n6811ens	307	6	Genbank	Genbank	NNP
cord-347472-n6811ens	307	7	files	file	NNS
cord-347472-n6811ens	307	8	for	for	IN
cord-347472-n6811ens	307	9	both	both	CC
cord-347472-n6811ens	307	10	RNA	RNA	NNP
cord-347472-n6811ens	307	11	(	(	-LRB-
cord-347472-n6811ens	307	12	CDS	CDS	NNP
cord-347472-n6811ens	307	13	)	)	-RRB-
cord-347472-n6811ens	307	14	and	and	CC
cord-347472-n6811ens	307	15	genomic	genomic	JJ
cord-347472-n6811ens	307	16	sequences	sequence	NNS
cord-347472-n6811ens	307	17	on	on	IN
cord-347472-n6811ens	307	18	the	the	DT
cord-347472-n6811ens	307	19	following	following	JJ
cord-347472-n6811ens	307	20	page	page	NN
cord-347472-n6811ens	307	21	.	.	.
cord-347472-n6811ens	308	1	Thank	thank	VBP
cord-347472-n6811ens	308	2	you	-PRON-	PRP
cord-347472-n6811ens	308	3	for	for	IN
cord-347472-n6811ens	308	4	your	-PRON-	PRP$
cord-347472-n6811ens	308	5	work	work	NN
cord-347472-n6811ens	308	6	on	on	IN
cord-347472-n6811ens	308	7	the	the	DT
cord-347472-n6811ens	308	8	rapid	rapid	JJ
cord-347472-n6811ens	308	9	development	development	NN
cord-347472-n6811ens	308	10	of	of	IN
cord-347472-n6811ens	308	11	this	this	DT
cord-347472-n6811ens	308	12	assay	assay	NN
cord-347472-n6811ens	308	13	;	;	:
cord-347472-n6811ens	308	14	my	-PRON-	PRP$
cord-347472-n6811ens	308	15	goal	goal	NN
cord-347472-n6811ens	308	16	in	in	IN
cord-347472-n6811ens	308	17	providing	provide	VBG
cord-347472-n6811ens	308	18	this	this	DT
cord-347472-n6811ens	308	19	feedback	feedback	NN
cord-347472-n6811ens	308	20	is	be	VBZ
cord-347472-n6811ens	308	21	to	to	TO
cord-347472-n6811ens	308	22	help	help	VB
cord-347472-n6811ens	308	23	improve	improve	VB
cord-347472-n6811ens	308	24	upon	upon	IN
cord-347472-n6811ens	308	25	your	-PRON-	PRP$
cord-347472-n6811ens	308	26	significant	significant	JJ
cord-347472-n6811ens	308	27	efforts	effort	NNS
cord-347472-n6811ens	308	28	and	and	CC
cord-347472-n6811ens	308	29	ensure	ensure	VB
cord-347472-n6811ens	308	30	that	that	IN
cord-347472-n6811ens	308	31	reported	report	VBD
cord-347472-n6811ens	308	32	results	result	NNS
cord-347472-n6811ens	308	33	reflect	reflect	VBP
cord-347472-n6811ens	308	34	patient	patient	JJ
cord-347472-n6811ens	308	35	disease	disease	NN
cord-347472-n6811ens	308	36	state	state	NN
cord-347472-n6811ens	308	37	.	.	.
cord-347472-n6811ens	309	1	Adam	Adam	NNP
cord-347472-n6811ens	309	2	Rosebrock	Rosebrock	NNP
cord-347472-n6811ens	309	3	,	,	,
cord-347472-n6811ens	309	4	PhD	phd	NN
cord-347472-n6811ens	309	5	CDC	CDC	NNP
cord-347472-n6811ens	309	6	's	's	POS
cord-347472-n6811ens	309	7	response	response	NN
cord-347472-n6811ens	309	8	,	,	,
cord-347472-n6811ens	309	9	received	receive	VBD
cord-347472-n6811ens	309	10	April	April	NNP
cord-347472-n6811ens	309	11	25	25	CD
cord-347472-n6811ens	309	12	,	,	,
cord-347472-n6811ens	309	13	2020	2020	CD
cord-347472-n6811ens	309	14	.	.	.
cord-347472-n6811ens	310	1	To	to	TO
cord-347472-n6811ens	310	2	protect	protect	VB
cord-347472-n6811ens	310	3	the	the	DT
cord-347472-n6811ens	310	4	email	email	NN
cord-347472-n6811ens	310	5	addresses	address	NNS
cord-347472-n6811ens	310	6	of	of	IN
cord-347472-n6811ens	310	7	those	those	DT
cord-347472-n6811ens	310	8	carbon	carbon	NN
cord-347472-n6811ens	310	9	copied	copy	VBN
cord-347472-n6811ens	310	10	,	,	,
cord-347472-n6811ens	310	11	CDC	CDC	NNP
cord-347472-n6811ens	310	12	-	-	HYPH
cord-347472-n6811ens	310	13	internal	internal	JJ
cord-347472-n6811ens	310	14	email	email	NN
cord-347472-n6811ens	310	15	addresses	address	NNS
cord-347472-n6811ens	310	16	have	have	VBP
cord-347472-n6811ens	310	17	been	be	VBN
cord-347472-n6811ens	310	18	redacted	redact	VBN
cord-347472-n6811ens	310	19	.	.	.
cord-347472-n6811ens	311	1	This	this	DT
cord-347472-n6811ens	311	2	email	email	NN
cord-347472-n6811ens	311	3	is	be	VBZ
cord-347472-n6811ens	311	4	being	be	VBG
cord-347472-n6811ens	311	5	presented	present	VBN
cord-347472-n6811ens	311	6	in	in	IN
cord-347472-n6811ens	311	7	full	full	JJ
cord-347472-n6811ens	311	8	and	and	CC
cord-347472-n6811ens	311	9	unedited	unedited	JJ
cord-347472-n6811ens	311	10	.	.	.
cord-347472-n6811ens	312	1	It	-PRON-	PRP
cord-347472-n6811ens	312	2	is	be	VBZ
cord-347472-n6811ens	312	3	internally	internally	RB
cord-347472-n6811ens	312	4	ambiguous	ambiguous	JJ
cord-347472-n6811ens	312	5	.	.	.
cord-347472-n6811ens	313	1	The	the	DT
cord-347472-n6811ens	313	2	first	first	JJ
cord-347472-n6811ens	313	3	paragraph	paragraph	NN
cord-347472-n6811ens	313	4	correctly	correctly	RB
cord-347472-n6811ens	313	5	notes	note	VBZ
cord-347472-n6811ens	313	6	that	that	IN
cord-347472-n6811ens	313	7	my	-PRON-	PRP$
cord-347472-n6811ens	313	8	email	email	NN
cord-347472-n6811ens	313	9	5	5	CD
cord-347472-n6811ens	313	10	reflected	reflect	VBN
cord-347472-n6811ens	313	11	concerns	concern	NNS
cord-347472-n6811ens	313	12	about	about	IN
cord-347472-n6811ens	313	13	false	false	JJ
cord-347472-n6811ens	313	14	negative	negative	JJ
cord-347472-n6811ens	313	15	potential	potential	NN
cord-347472-n6811ens	313	16	.	.	.
cord-347472-n6811ens	314	1	This	this	DT
cord-347472-n6811ens	314	2	is	be	VBZ
cord-347472-n6811ens	314	3	contradicted	contradict	VBN
cord-347472-n6811ens	314	4	in	in	IN
cord-347472-n6811ens	314	5	the	the	DT
cord-347472-n6811ens	314	6	second	second	JJ
cord-347472-n6811ens	314	7	paragraph	paragraph	NN
cord-347472-n6811ens	314	8	as	as	IN
cord-347472-n6811ens	314	9	"	"	``
cord-347472-n6811ens	314	10	false	false	JJ
cord-347472-n6811ens	314	11	-	-	HYPH
cord-347472-n6811ens	314	12	positive	positive	JJ
cord-347472-n6811ens	314	13	results	result	NNS
cord-347472-n6811ens	314	14	"	"	''
cord-347472-n6811ens	314	15	,	,	,
cord-347472-n6811ens	314	16	which	which	WDT
cord-347472-n6811ens	314	17	I	-PRON-	PRP
cord-347472-n6811ens	314	18	hope	hope	VBP
cord-347472-n6811ens	314	19	to	to	TO
cord-347472-n6811ens	314	20	be	be	VB
cord-347472-n6811ens	314	21	a	a	DT
cord-347472-n6811ens	314	22	typo	typo	NN
cord-347472-n6811ens	314	23	by	by	IN
cord-347472-n6811ens	314	24	the	the	DT
cord-347472-n6811ens	314	25	CDC	CDC	NNP
cord-347472-n6811ens	314	26	.	.	.
cord-347472-n6811ens	315	1	Given	give	VBN
cord-347472-n6811ens	315	2	the	the	DT
cord-347472-n6811ens	315	3	importance	importance	NN
cord-347472-n6811ens	315	4	of	of	IN
cord-347472-n6811ens	315	5	this	this	DT
cord-347472-n6811ens	315	6	communication	communication	NN
cord-347472-n6811ens	315	7	in	in	IN
cord-347472-n6811ens	315	8	the	the	DT
cord-347472-n6811ens	315	9	context	context	NN
cord-347472-n6811ens	315	10	of	of	IN
cord-347472-n6811ens	315	11	CDC	CDC	NNP
cord-347472-n6811ens	315	12	's	's	POS
cord-347472-n6811ens	315	13	response	response	NN
cord-347472-n6811ens	315	14	,	,	,
cord-347472-n6811ens	315	15	I	-PRON-	PRP
cord-347472-n6811ens	315	16	am	be	VBP
cord-347472-n6811ens	315	17	including	include	VBG
cord-347472-n6811ens	315	18	a	a	DT
cord-347472-n6811ens	315	19	contact	contact	NN
cord-347472-n6811ens	315	20	-	-	HYPH
cord-347472-n6811ens	315	21	info	info	NN
cord-347472-n6811ens	315	22	redacted	redact	VBN
cord-347472-n6811ens	315	23	version	version	NN
cord-347472-n6811ens	315	24	of	of	IN
cord-347472-n6811ens	315	25	this	this	DT
cord-347472-n6811ens	315	26	email	email	NN
cord-347472-n6811ens	315	27	in	in	IN
cord-347472-n6811ens	315	28	spite	spite	NN
cord-347472-n6811ens	315	29	of	of	IN
cord-347472-n6811ens	315	30	the	the	DT
cord-347472-n6811ens	315	31	"	"	``
cord-347472-n6811ens	315	32	do	do	VBP
cord-347472-n6811ens	315	33	not	not	RB
cord-347472-n6811ens	315	34	distribute	distribute	VB
cord-347472-n6811ens	315	35	"	"	``
cord-347472-n6811ens	315	36	message	message	NN
cord-347472-n6811ens	315	37	.	.	.
cord-347472-n6811ens	316	1	No	no	DT
cord-347472-n6811ens	316	2	patient	patient	JJ
cord-347472-n6811ens	316	3	identifying	identify	VBG
cord-347472-n6811ens	316	4	information	information	NN
cord-347472-n6811ens	316	5	is	be	VBZ
cord-347472-n6811ens	316	6	present	present	JJ
cord-347472-n6811ens	316	7	,	,	,
cord-347472-n6811ens	316	8	and	and	CC
cord-347472-n6811ens	316	9	this	this	DT
cord-347472-n6811ens	316	10	represents	represent	VBZ
cord-347472-n6811ens	316	11	US	US	NNP
cord-347472-n6811ens	316	12	government	government	NN
cord-347472-n6811ens	316	13	work	work	NN
cord-347472-n6811ens	316	14	product	product	NN
cord-347472-n6811ens	316	15	(	(	-LRB-
cord-347472-n6811ens	316	16	from	from	IN
cord-347472-n6811ens	316	17	the	the	DT
cord-347472-n6811ens	316	18	CDC	CDC	NNP
cord-347472-n6811ens	316	19	)	)	-RRB-
cord-347472-n6811ens	316	20	and	and	CC
cord-347472-n6811ens	316	21	that	that	DT
cord-347472-n6811ens	316	22	of	of	IN
cord-347472-n6811ens	316	23	a	a	DT
cord-347472-n6811ens	316	24	NY	NY	NNP
cord-347472-n6811ens	316	25	state	state	NN
cord-347472-n6811ens	316	26	,	,	,
cord-347472-n6811ens	316	27	federally	federally	RB
cord-347472-n6811ens	316	28	grant	grant	VB
cord-347472-n6811ens	316	29	funded	fund	VBN
cord-347472-n6811ens	316	30	employee	employee	NN
cord-347472-n6811ens	316	31	(	(	-LRB-
cord-347472-n6811ens	316	32	the	the	DT
cord-347472-n6811ens	316	33	recipient	recipient	NN
cord-347472-n6811ens	316	34	)	)	-RRB-
cord-347472-n6811ens	316	35	.	.	.
cord-347472-n6811ens	317	1	Dear	dear	JJ
cord-347472-n6811ens	317	2	Adam	Adam	NNP
cord-347472-n6811ens	317	3	Rosebrock	Rosebrock	NNP
cord-347472-n6811ens	317	4	,	,	,
cord-347472-n6811ens	317	5	Our	-PRON-	PRP$
cord-347472-n6811ens	317	6	apologies	apology	NNS
cord-347472-n6811ens	317	7	for	for	IN
cord-347472-n6811ens	317	8	the	the	DT
cord-347472-n6811ens	317	9	delayed	delay	VBN
cord-347472-n6811ens	317	10	response	response	NN
cord-347472-n6811ens	317	11	to	to	IN
cord-347472-n6811ens	317	12	your	-PRON-	PRP$
cord-347472-n6811ens	317	13	email	email	NN
cord-347472-n6811ens	317	14	.	.	.
cord-347472-n6811ens	318	1	As	as	IN
cord-347472-n6811ens	318	2	you	-PRON-	PRP
cord-347472-n6811ens	318	3	can	can	MD
cord-347472-n6811ens	318	4	imagine	imagine	VB
cord-347472-n6811ens	318	5	we	-PRON-	PRP
cord-347472-n6811ens	318	6	are	be	VBP
cord-347472-n6811ens	318	7	currently	currently	RB
cord-347472-n6811ens	318	8	experiencing	experience	VBG
cord-347472-n6811ens	318	9	a	a	DT
cord-347472-n6811ens	318	10	high	high	JJ
cord-347472-n6811ens	318	11	volume	volume	NN
cord-347472-n6811ens	318	12	of	of	IN
cord-347472-n6811ens	318	13	inquiries	inquiry	NNS
cord-347472-n6811ens	318	14	,	,	,
cord-347472-n6811ens	318	15	but	but	CC
cord-347472-n6811ens	318	16	patient	patient	JJ
cord-347472-n6811ens	318	17	care	care	NN
cord-347472-n6811ens	318	18	is	be	VBZ
cord-347472-n6811ens	318	19	a	a	DT
cord-347472-n6811ens	318	20	top	top	JJ
cord-347472-n6811ens	318	21	priority	priority	NN
cord-347472-n6811ens	318	22	and	and	CC
cord-347472-n6811ens	318	23	we	-PRON-	PRP
cord-347472-n6811ens	318	24	want	want	VBP
cord-347472-n6811ens	318	25	to	to	TO
cord-347472-n6811ens	318	26	thank	thank	VB
cord-347472-n6811ens	318	27	you	-PRON-	PRP
cord-347472-n6811ens	318	28	for	for	IN
cord-347472-n6811ens	318	29	reaching	reach	VBG
cord-347472-n6811ens	318	30	out	out	RP
cord-347472-n6811ens	318	31	to	to	IN
cord-347472-n6811ens	318	32	us	-PRON-	PRP
cord-347472-n6811ens	318	33	about	about	IN
cord-347472-n6811ens	318	34	your	-PRON-	PRP$
cord-347472-n6811ens	318	35	concerns	concern	NNS
cord-347472-n6811ens	318	36	for	for	IN
cord-347472-n6811ens	318	37	the	the	DT
cord-347472-n6811ens	318	38	2019-nCov	2019-ncov	CD
cord-347472-n6811ens	318	39	assay	assay	NN
cord-347472-n6811ens	318	40	and	and	CC
cord-347472-n6811ens	318	41	the	the	DT
cord-347472-n6811ens	318	42	potential	potential	NN
cord-347472-n6811ens	318	43	for	for	IN
cord-347472-n6811ens	318	44	false	false	JJ
cord-347472-n6811ens	318	45	negatives	negative	NNS
cord-347472-n6811ens	318	46	.	.	.
cord-347472-n6811ens	319	1	The	the	DT
cord-347472-n6811ens	319	2	assay	assay	NN
cord-347472-n6811ens	319	3	RNaseP	RNaseP	NNP
cord-347472-n6811ens	319	4	control	control	NN
cord-347472-n6811ens	319	5	serves	serve	VBZ
cord-347472-n6811ens	319	6	as	as	IN
cord-347472-n6811ens	319	7	an	an	DT
cord-347472-n6811ens	319	8	extraction	extraction	NN
cord-347472-n6811ens	319	9	(	(	-LRB-
cord-347472-n6811ens	319	10	total	total	JJ
cord-347472-n6811ens	319	11	nucleic	nucleic	JJ
cord-347472-n6811ens	319	12	acid	acid	NN
cord-347472-n6811ens	319	13	)	)	-RRB-
cord-347472-n6811ens	319	14	and	and	CC
cord-347472-n6811ens	319	15	amplification	amplification	NN
cord-347472-n6811ens	319	16	control	control	NN
cord-347472-n6811ens	319	17	.	.	.
cord-347472-n6811ens	320	1	The	the	DT
cord-347472-n6811ens	320	2	assay	assay	NN
cord-347472-n6811ens	320	3	positive	positive	JJ
cord-347472-n6811ens	320	4	control	control	NN
cord-347472-n6811ens	320	5	serves	serve	VBZ
cord-347472-n6811ens	320	6	as	as	IN
cord-347472-n6811ens	320	7	a	a	DT
cord-347472-n6811ens	320	8	control	control	NN
cord-347472-n6811ens	320	9	for	for	IN
cord-347472-n6811ens	320	10	Reverse	Reverse	NNP
cord-347472-n6811ens	320	11	Transcriptase	Transcriptase	NNP
cord-347472-n6811ens	320	12	function	function	NN
cord-347472-n6811ens	320	13	.	.	.
cord-347472-n6811ens	321	1	Although	although	IN
cord-347472-n6811ens	321	2	there	there	EX
cord-347472-n6811ens	321	3	is	be	VBZ
cord-347472-n6811ens	321	4	a	a	DT
cord-347472-n6811ens	321	5	theoretical	theoretical	JJ
cord-347472-n6811ens	321	6	possibility	possibility	NN
cord-347472-n6811ens	321	7	that	that	WDT
cord-347472-n6811ens	321	8	extracted	extract	VBD
cord-347472-n6811ens	321	9	RNA	RNA	NNP
cord-347472-n6811ens	321	10	might	may	MD
cord-347472-n6811ens	321	11	be	be	VB
cord-347472-n6811ens	321	12	degraded	degrade	VBN
cord-347472-n6811ens	321	13	in	in	IN
cord-347472-n6811ens	321	14	individual	individual	JJ
cord-347472-n6811ens	321	15	samples	sample	NNS
cord-347472-n6811ens	321	16	,	,	,
cord-347472-n6811ens	321	17	this	this	DT
cord-347472-n6811ens	321	18	assay	assay	NN
cord-347472-n6811ens	321	19	design	design	NN
cord-347472-n6811ens	321	20	has	have	VBZ
cord-347472-n6811ens	321	21	been	be	VBN
cord-347472-n6811ens	321	22	authorized	authorize	VBN
cord-347472-n6811ens	321	23	by	by	IN
cord-347472-n6811ens	321	24	FDA	FDA	NNP
cord-347472-n6811ens	321	25	.	.	.
cord-347472-n6811ens	322	1	In	in	IN
cord-347472-n6811ens	322	2	addition	addition	NN
cord-347472-n6811ens	322	3	,	,	,
cord-347472-n6811ens	322	4	CDC	CDC	NNP
cord-347472-n6811ens	322	5	has	have	VBZ
cord-347472-n6811ens	322	6	not	not	RB
cord-347472-n6811ens	322	7	received	receive	VBN
cord-347472-n6811ens	322	8	feedback	feedback	NN
cord-347472-n6811ens	322	9	that	that	IN
cord-347472-n6811ens	322	10	the	the	DT
cord-347472-n6811ens	323	1	CDC	CDC	NNP
cord-347472-n6811ens	323	2	EUA	EUA	NNP
cord-347472-n6811ens	323	3	COVID-19	COVID-19	NNP
cord-347472-n6811ens	323	4	assay	assay	NN
cord-347472-n6811ens	323	5	produces	produce	VBZ
cord-347472-n6811ens	323	6	false	false	JJ
cord-347472-n6811ens	323	7	-	-	HYPH
cord-347472-n6811ens	323	8	positive	positive	JJ
cord-347472-n6811ens	323	9	results	result	NNS
cord-347472-n6811ens	323	10	when	when	WRB
cord-347472-n6811ens	323	11	used	use	VBN
cord-347472-n6811ens	323	12	according	accord	VBG
cord-347472-n6811ens	323	13	to	to	IN
cord-347472-n6811ens	323	14	the	the	DT
cord-347472-n6811ens	323	15	IFU	IFU	NNP
cord-347472-n6811ens	323	16	.	.	.
cord-347472-n6811ens	324	1	The	the	DT
cord-347472-n6811ens	324	2	CDC	CDC	NNP
cord-347472-n6811ens	324	3	notes	note	NNS
cord-347472-n6811ens	324	4	on	on	IN
cord-347472-n6811ens	324	5	its	-PRON-	PRP$
cord-347472-n6811ens	324	6	website	website	NN
cord-347472-n6811ens	324	7	and	and	CC
cord-347472-n6811ens	324	8	in	in	IN
cord-347472-n6811ens	324	9	its	-PRON-	PRP$
cord-347472-n6811ens	324	10	advice	advice	NN
cord-347472-n6811ens	324	11	to	to	IN
cord-347472-n6811ens	324	12	both	both	DT
cord-347472-n6811ens	324	13	physicians	physician	NNS
cord-347472-n6811ens	324	14	and	and	CC
cord-347472-n6811ens	324	15	patients	patient	NNS
cord-347472-n6811ens	324	16	that	that	IN
cord-347472-n6811ens	324	17	the	the	DT
cord-347472-n6811ens	324	18	RT	RT	NNP
cord-347472-n6811ens	324	19	-	-	HYPH
cord-347472-n6811ens	324	20	PCR	PCR	NNP
cord-347472-n6811ens	324	21	may	may	MD
cord-347472-n6811ens	324	22	come	come	VB
cord-347472-n6811ens	324	23	back	back	RB
cord-347472-n6811ens	324	24	negative	negative	JJ
cord-347472-n6811ens	324	25	if	if	IN
cord-347472-n6811ens	324	26	viral	viral	JJ
cord-347472-n6811ens	324	27	titers	titer	NNS
cord-347472-n6811ens	324	28	have	have	VBP
cord-347472-n6811ens	324	29	n't	not	RB
cord-347472-n6811ens	324	30	yet	yet	RB
cord-347472-n6811ens	324	31	reached	reach	VBN
cord-347472-n6811ens	324	32	the	the	DT
cord-347472-n6811ens	324	33	levels	level	NNS
cord-347472-n6811ens	324	34	of	of	IN
cord-347472-n6811ens	324	35	detection	detection	NN
cord-347472-n6811ens	324	36	,	,	,
cord-347472-n6811ens	324	37	or	or	CC
cord-347472-n6811ens	324	38	potentially	potentially	RB
cord-347472-n6811ens	324	39	if	if	IN
cord-347472-n6811ens	324	40	the	the	DT
cord-347472-n6811ens	324	41	RNA	RNA	NNP
cord-347472-n6811ens	324	42	has	have	VBZ
cord-347472-n6811ens	324	43	degraded	degrade	VBN
cord-347472-n6811ens	324	44	as	as	IN
cord-347472-n6811ens	324	45	is	be	VBZ
cord-347472-n6811ens	324	46	your	-PRON-	PRP$
cord-347472-n6811ens	324	47	concern	concern	NN
cord-347472-n6811ens	324	48	,	,	,
cord-347472-n6811ens	324	49	and	and	CC
cord-347472-n6811ens	324	50	to	to	TO
cord-347472-n6811ens	324	51	always	always	RB
cord-347472-n6811ens	324	52	factor	factor	VB
cord-347472-n6811ens	324	53	in	in	IN
cord-347472-n6811ens	324	54	the	the	DT
cord-347472-n6811ens	324	55	patient	patient	NN
cord-347472-n6811ens	324	56	's	's	POS
cord-347472-n6811ens	324	57	symptoms	symptom	NNS
cord-347472-n6811ens	324	58	when	when	WRB
cord-347472-n6811ens	324	59	making	make	VBG
cord-347472-n6811ens	324	60	a	a	DT
cord-347472-n6811ens	324	61	diagnosis	diagnosis	NN
cord-347472-n6811ens	324	62	.	.	.
cord-347472-n6811ens	325	1	I	-PRON-	PRP
cord-347472-n6811ens	325	2	believe	believe	VBP
cord-347472-n6811ens	325	3	we	-PRON-	PRP
cord-347472-n6811ens	325	4	can	can	MD
cord-347472-n6811ens	325	5	all	all	RB
cord-347472-n6811ens	325	6	agree	agree	VB
cord-347472-n6811ens	325	7	that	that	IN
cord-347472-n6811ens	325	8	in	in	IN
cord-347472-n6811ens	325	9	this	this	DT
cord-347472-n6811ens	325	10	pandemic	pandemic	NN
cord-347472-n6811ens	325	11	it	-PRON-	PRP
cord-347472-n6811ens	325	12	is	be	VBZ
cord-347472-n6811ens	325	13	better	well	JJR
cord-347472-n6811ens	325	14	to	to	TO
cord-347472-n6811ens	325	15	err	err	VB
cord-347472-n6811ens	325	16	on	on	IN
cord-347472-n6811ens	325	17	the	the	DT
cord-347472-n6811ens	325	18	side	side	NN
cord-347472-n6811ens	325	19	of	of	IN
cord-347472-n6811ens	325	20	caution	caution	NN
cord-347472-n6811ens	325	21	.	.	.
cord-347472-n6811ens	326	1	I	-PRON-	PRP
cord-347472-n6811ens	326	2	hope	hope	VBP
cord-347472-n6811ens	326	3	this	this	DT
cord-347472-n6811ens	326	4	response	response	NN
cord-347472-n6811ens	326	5	answers	answer	VBZ
cord-347472-n6811ens	326	6	some	some	DT
cord-347472-n6811ens	326	7	of	of	IN
cord-347472-n6811ens	326	8	your	-PRON-	PRP$
cord-347472-n6811ens	326	9	concerns	concern	NNS
cord-347472-n6811ens	326	10	,	,	,
cord-347472-n6811ens	326	11	and	and	CC
cord-347472-n6811ens	326	12	have	have	VBP
cord-347472-n6811ens	326	13	a	a	DT
cord-347472-n6811ens	326	14	wonderful	wonderful	JJ
cord-347472-n6811ens	326	15	day	day	NN
cord-347472-n6811ens	326	16	!	!	.
cord-347472-n6811ens	327	1	This	this	DT
cord-347472-n6811ens	327	2	e	e	NN
cord-347472-n6811ens	327	3	-	-	NN
cord-347472-n6811ens	327	4	mail	mail	NN
cord-347472-n6811ens	327	5	message	message	NN
cord-347472-n6811ens	327	6	,	,	,
cord-347472-n6811ens	327	7	including	include	VBG
cord-347472-n6811ens	327	8	any	any	DT
cord-347472-n6811ens	327	9	attachments	attachment	NNS
cord-347472-n6811ens	327	10	,	,	,
cord-347472-n6811ens	327	11	is	be	VBZ
cord-347472-n6811ens	327	12	for	for	IN
cord-347472-n6811ens	327	13	the	the	DT
cord-347472-n6811ens	327	14	sole	sole	JJ
cord-347472-n6811ens	327	15	use	use	NN
cord-347472-n6811ens	327	16	of	of	IN
cord-347472-n6811ens	327	17	the	the	DT
cord-347472-n6811ens	327	18	intended	intended	JJ
cord-347472-n6811ens	327	19	recipient(s	recipient(s	NNP
cord-347472-n6811ens	327	20	)	)	-RRB-
cord-347472-n6811ens	327	21	and	and	CC
cord-347472-n6811ens	327	22	may	may	MD
cord-347472-n6811ens	327	23	contain	contain	VB
cord-347472-n6811ens	327	24	confidential	confidential	JJ
cord-347472-n6811ens	327	25	and	and	CC
cord-347472-n6811ens	327	26	privileged	privileged	JJ
cord-347472-n6811ens	327	27	information	information	NN
cord-347472-n6811ens	327	28	.	.	.
cord-347472-n6811ens	328	1	Any	any	DT
cord-347472-n6811ens	328	2	unauthorized	unauthorized	JJ
cord-347472-n6811ens	328	3	review	review	NN
cord-347472-n6811ens	328	4	,	,	,
cord-347472-n6811ens	328	5	use	use	NN
cord-347472-n6811ens	328	6	,	,	,
cord-347472-n6811ens	328	7	disclosure	disclosure	NN
cord-347472-n6811ens	328	8	or	or	CC
cord-347472-n6811ens	328	9	distribution	distribution	NN
cord-347472-n6811ens	328	10	is	be	VBZ
cord-347472-n6811ens	328	11	prohibited	prohibit	VBN
cord-347472-n6811ens	328	12	.	.	.
cord-347472-n6811ens	329	1	If	if	IN
cord-347472-n6811ens	329	2	you	-PRON-	PRP
cord-347472-n6811ens	329	3	are	be	VBP
cord-347472-n6811ens	329	4	not	not	RB
cord-347472-n6811ens	329	5	the	the	DT
cord-347472-n6811ens	329	6	intended	intend	VBN
cord-347472-n6811ens	329	7	recipient	recipient	NN
cord-347472-n6811ens	329	8	,	,	,
cord-347472-n6811ens	329	9	please	please	UH
cord-347472-n6811ens	329	10	contact	contact	VB
cord-347472-n6811ens	329	11	the	the	DT
cord-347472-n6811ens	329	12	sender	sender	NN
cord-347472-n6811ens	329	13	by	by	IN
cord-347472-n6811ens	329	14	e	e	NN
cord-347472-n6811ens	329	15	-	-	NN
cord-347472-n6811ens	329	16	mail	mail	NN
cord-347472-n6811ens	329	17	and	and	CC
cord-347472-n6811ens	329	18	destroy	destroy	VB
cord-347472-n6811ens	329	19	all	all	DT
cord-347472-n6811ens	329	20	copies	copy	NNS
cord-347472-n6811ens	329	21	of	of	IN
cord-347472-n6811ens	329	22	the	the	DT
cord-347472-n6811ens	329	23	original	original	JJ
cord-347472-n6811ens	329	24	.	.	.
cord-347472-n6811ens	330	1	Virological	virological	JJ
cord-347472-n6811ens	330	2	assessment	assessment	NN
cord-347472-n6811ens	330	3	of	of	IN
cord-347472-n6811ens	330	4	hospitalized	hospitalize	VBN
cord-347472-n6811ens	330	5	patients	patient	NNS
cord-347472-n6811ens	330	6	with	with	IN
cord-347472-n6811ens	330	7	COVID-2019	COVID-2019	NNP
cord-347472-n6811ens	330	8	Revision	Revision	NNP
cord-347472-n6811ens	330	9	:	:	:
cord-347472-n6811ens	330	10	03	03	CD
cord-347472-n6811ens	330	11	,	,	,
cord-347472-n6811ens	330	12	Centers	Centers	NNPS
cord-347472-n6811ens	330	13	for	for	IN
cord-347472-n6811ens	330	14	Disease	Disease	NNP
cord-347472-n6811ens	330	15	Control	Control	NNP
cord-347472-n6811ens	330	16	and	and	CC
cord-347472-n6811ens	330	17	Prevention	Prevention	NNP
cord-347472-n6811ens	330	18	:	:	:
cord-347472-n6811ens	330	19	Division	division	NN
cord-347472-n6811ens	330	20	of	of	IN
cord-347472-n6811ens	330	21	Viral	Viral	NNP
cord-347472-n6811ens	330	22	Diseases	Diseases	NNPS
cord-347472-n6811ens	330	23	Acknowledgments	acknowledgment	NNS
cord-347472-n6811ens	331	1	:	:	:
cord-347472-n6811ens	331	2	APR	APR	NNP
cord-347472-n6811ens	331	3	would	would	MD
cord-347472-n6811ens	331	4	like	like	VB
cord-347472-n6811ens	331	5	to	to	TO
cord-347472-n6811ens	331	6	thank	thank	VB
cord-347472-n6811ens	331	7	Amy	Amy	NNP
cord-347472-n6811ens	331	8	A.	A.	NNP
cord-347472-n6811ens	331	9	Caudy	Caudy	NNP
cord-347472-n6811ens	331	10	for	for	IN
cord-347472-n6811ens	331	11	technical	technical	JJ
cord-347472-n6811ens	331	12	discussion	discussion	NN
cord-347472-n6811ens	331	13	and	and	CC
cord-347472-n6811ens	331	14	critical	critical	JJ
cord-347472-n6811ens	331	15	reading	reading	NN
cord-347472-n6811ens	331	16	of	of	IN
cord-347472-n6811ens	331	17	the	the	DT
cord-347472-n6811ens	331	18	manuscript	manuscript	NN
cord-347472-n6811ens	331	19	,	,	,
cord-347472-n6811ens	331	20	Bruce	Bruce	NNP
cord-347472-n6811ens	331	21	Futcher	Futcher	NNP
cord-347472-n6811ens	331	22	for	for	IN
cord-347472-n6811ens	331	23	discussion	discussion	NN
cord-347472-n6811ens	331	24	,	,	,
cord-347472-n6811ens	331	25	and	and	CC
cord-347472-n6811ens	331	26	Richard	Richard	NNP
cord-347472-n6811ens	331	27	Kew	Kew	NNP
cord-347472-n6811ens	331	28	and	and	CC
cord-347472-n6811ens	331	29	Karen	Karen	NNP
cord-347472-n6811ens	331	30	Bai	Bai	NNP