id	sid	tid	token	lemma	pos
cord-328460-thx9zh11	1	1	key	key	NN
cord-328460-thx9zh11	1	2	:	:	:
cord-328460-thx9zh11	2	1	cord-328460-thx9zh11	cord-328460-thx9zh11	NNP
cord-328460-thx9zh11	3	1	authors	author	NNS
cord-328460-thx9zh11	3	2	:	:	:
cord-328460-thx9zh11	3	3	Zanoli	Zanoli	NNP
cord-328460-thx9zh11	3	4	,	,	,
cord-328460-thx9zh11	3	5	Laura	Laura	NNP
cord-328460-thx9zh11	3	6	Maria	Maria	NNP
cord-328460-thx9zh11	3	7	;	;	:
cord-328460-thx9zh11	3	8	Spoto	Spoto	NNP
cord-328460-thx9zh11	3	9	,	,	,
cord-328460-thx9zh11	3	10	Giuseppe	Giuseppe	NNP
cord-328460-thx9zh11	3	11	title	title	NN
cord-328460-thx9zh11	3	12	:	:	:
cord-328460-thx9zh11	3	13	Isothermal	Isothermal	NNP
cord-328460-thx9zh11	3	14	Amplification	Amplification	NNP
cord-328460-thx9zh11	3	15	Methods	Methods	NNPS
cord-328460-thx9zh11	3	16	for	for	IN
cord-328460-thx9zh11	3	17	the	the	DT
cord-328460-thx9zh11	3	18	Detection	detection	NN
cord-328460-thx9zh11	3	19	of	of	IN
cord-328460-thx9zh11	3	20	Nucleic	nucleic	JJ
cord-328460-thx9zh11	3	21	Acids	acid	NNS
cord-328460-thx9zh11	3	22	in	in	IN
cord-328460-thx9zh11	3	23	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	3	24	Devices	Devices	NNPS
cord-328460-thx9zh11	3	25	date	date	NN
cord-328460-thx9zh11	3	26	:	:	:
cord-328460-thx9zh11	3	27	2012	2012	CD
cord-328460-thx9zh11	3	28	-	-	SYM
cord-328460-thx9zh11	3	29	12	12	CD
cord-328460-thx9zh11	3	30	-	-	SYM
cord-328460-thx9zh11	3	31	27	27	CD
cord-328460-thx9zh11	3	32	journal	journal	NN
cord-328460-thx9zh11	3	33	:	:	:
cord-328460-thx9zh11	3	34	Biosensors	Biosensors	NNP
cord-328460-thx9zh11	3	35	(	(	-LRB-
cord-328460-thx9zh11	3	36	Basel	Basel	NNP
cord-328460-thx9zh11	3	37	)	)	-RRB-
cord-328460-thx9zh11	3	38	DOI	DOI	NNP
cord-328460-thx9zh11	3	39	:	:	:
cord-328460-thx9zh11	4	1	10.3390	10.3390	CD
cord-328460-thx9zh11	4	2	/	/	SYM
cord-328460-thx9zh11	4	3	bios3010018	bios3010018	NNP
cord-328460-thx9zh11	4	4	sha	sha	NNP
cord-328460-thx9zh11	4	5	:	:	:
cord-328460-thx9zh11	5	1	1624c6455f4fb4a8e871403b0e27a217bcdc83b2	1624c6455f4fb4a8e871403b0e27a217bcdc83b2	LS
cord-328460-thx9zh11	5	2	doc_id	doc_id	CD
cord-328460-thx9zh11	5	3	:	:	:
cord-328460-thx9zh11	5	4	328460	328460	CD
cord-328460-thx9zh11	5	5	cord_uid	cord_uid	NNS
cord-328460-thx9zh11	5	6	:	:	:
cord-328460-thx9zh11	5	7	thx9zh11	thx9zh11	NNP
cord-328460-thx9zh11	5	8	Diagnostic	Diagnostic	NNP
cord-328460-thx9zh11	5	9	tools	tool	NNS
cord-328460-thx9zh11	5	10	for	for	IN
cord-328460-thx9zh11	5	11	biomolecular	biomolecular	JJ
cord-328460-thx9zh11	5	12	detection	detection	NN
cord-328460-thx9zh11	5	13	need	need	VBP
cord-328460-thx9zh11	5	14	to	to	TO
cord-328460-thx9zh11	5	15	fulfill	fulfill	VB
cord-328460-thx9zh11	5	16	specific	specific	JJ
cord-328460-thx9zh11	5	17	requirements	requirement	NNS
cord-328460-thx9zh11	5	18	in	in	IN
cord-328460-thx9zh11	5	19	terms	term	NNS
cord-328460-thx9zh11	5	20	of	of	IN
cord-328460-thx9zh11	5	21	sensitivity	sensitivity	NN
cord-328460-thx9zh11	5	22	,	,	,
cord-328460-thx9zh11	5	23	selectivity	selectivity	NN
cord-328460-thx9zh11	5	24	and	and	CC
cord-328460-thx9zh11	5	25	high	high	JJ
cord-328460-thx9zh11	5	26	-	-	HYPH
cord-328460-thx9zh11	5	27	throughput	throughput	NN
cord-328460-thx9zh11	5	28	in	in	IN
cord-328460-thx9zh11	5	29	order	order	NN
cord-328460-thx9zh11	5	30	to	to	TO
cord-328460-thx9zh11	5	31	widen	widen	VB
cord-328460-thx9zh11	5	32	their	-PRON-	PRP$
cord-328460-thx9zh11	5	33	applicability	applicability	NN
cord-328460-thx9zh11	5	34	and	and	CC
cord-328460-thx9zh11	5	35	to	to	TO
cord-328460-thx9zh11	5	36	minimize	minimize	VB
cord-328460-thx9zh11	5	37	the	the	DT
cord-328460-thx9zh11	5	38	cost	cost	NN
cord-328460-thx9zh11	5	39	of	of	IN
cord-328460-thx9zh11	5	40	the	the	DT
cord-328460-thx9zh11	5	41	assay	assay	NN
cord-328460-thx9zh11	5	42	.	.	.
cord-328460-thx9zh11	6	1	The	the	DT
cord-328460-thx9zh11	6	2	nucleic	nucleic	JJ
cord-328460-thx9zh11	6	3	acid	acid	NN
cord-328460-thx9zh11	6	4	amplification	amplification	NN
cord-328460-thx9zh11	6	5	is	be	VBZ
cord-328460-thx9zh11	6	6	a	a	DT
cord-328460-thx9zh11	6	7	key	key	JJ
cord-328460-thx9zh11	6	8	step	step	NN
cord-328460-thx9zh11	6	9	in	in	IN
cord-328460-thx9zh11	6	10	DNA	dna	NN
cord-328460-thx9zh11	6	11	detection	detection	NN
cord-328460-thx9zh11	6	12	assays	assay	NNS
cord-328460-thx9zh11	6	13	.	.	.
cord-328460-thx9zh11	7	1	It	-PRON-	PRP
cord-328460-thx9zh11	7	2	contributes	contribute	VBZ
cord-328460-thx9zh11	7	3	to	to	IN
cord-328460-thx9zh11	7	4	improving	improve	VBG
cord-328460-thx9zh11	7	5	the	the	DT
cord-328460-thx9zh11	7	6	assay	assay	NN
cord-328460-thx9zh11	7	7	sensitivity	sensitivity	NN
cord-328460-thx9zh11	7	8	by	by	IN
cord-328460-thx9zh11	7	9	enabling	enable	VBG
cord-328460-thx9zh11	7	10	the	the	DT
cord-328460-thx9zh11	7	11	detection	detection	NN
cord-328460-thx9zh11	7	12	of	of	IN
cord-328460-thx9zh11	7	13	a	a	DT
cord-328460-thx9zh11	7	14	limited	limited	JJ
cord-328460-thx9zh11	7	15	number	number	NN
cord-328460-thx9zh11	7	16	of	of	IN
cord-328460-thx9zh11	7	17	target	target	NN
cord-328460-thx9zh11	7	18	molecules	molecule	NNS
cord-328460-thx9zh11	7	19	.	.	.
cord-328460-thx9zh11	8	1	The	the	DT
cord-328460-thx9zh11	8	2	use	use	NN
cord-328460-thx9zh11	8	3	of	of	IN
cord-328460-thx9zh11	8	4	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	8	5	devices	device	NNS
cord-328460-thx9zh11	8	6	to	to	TO
cord-328460-thx9zh11	8	7	miniaturize	miniaturize	VB
cord-328460-thx9zh11	8	8	amplification	amplification	NN
cord-328460-thx9zh11	8	9	protocols	protocol	NNS
cord-328460-thx9zh11	8	10	reduces	reduce	VBZ
cord-328460-thx9zh11	8	11	the	the	DT
cord-328460-thx9zh11	8	12	required	require	VBN
cord-328460-thx9zh11	8	13	sample	sample	NN
cord-328460-thx9zh11	8	14	volume	volume	NN
cord-328460-thx9zh11	8	15	and	and	CC
cord-328460-thx9zh11	8	16	the	the	DT
cord-328460-thx9zh11	8	17	analysis	analysis	NN
cord-328460-thx9zh11	8	18	times	time	NNS
cord-328460-thx9zh11	8	19	and	and	CC
cord-328460-thx9zh11	8	20	offers	offer	VBZ
cord-328460-thx9zh11	8	21	new	new	JJ
cord-328460-thx9zh11	8	22	possibilities	possibility	NNS
cord-328460-thx9zh11	8	23	for	for	IN
cord-328460-thx9zh11	8	24	the	the	DT
cord-328460-thx9zh11	8	25	process	process	NN
cord-328460-thx9zh11	8	26	automation	automation	NN
cord-328460-thx9zh11	8	27	and	and	CC
cord-328460-thx9zh11	8	28	integration	integration	NN
cord-328460-thx9zh11	8	29	in	in	IN
cord-328460-thx9zh11	8	30	one	one	CD
cord-328460-thx9zh11	8	31	single	single	JJ
cord-328460-thx9zh11	8	32	device	device	NN
cord-328460-thx9zh11	8	33	.	.	.
cord-328460-thx9zh11	9	1	The	the	DT
cord-328460-thx9zh11	9	2	vast	vast	JJ
cord-328460-thx9zh11	9	3	majority	majority	NN
cord-328460-thx9zh11	9	4	of	of	IN
cord-328460-thx9zh11	9	5	miniaturized	miniaturized	JJ
cord-328460-thx9zh11	9	6	systems	system	NNS
cord-328460-thx9zh11	9	7	for	for	IN
cord-328460-thx9zh11	9	8	nucleic	nucleic	JJ
cord-328460-thx9zh11	9	9	acid	acid	NN
cord-328460-thx9zh11	9	10	analysis	analysis	NN
cord-328460-thx9zh11	9	11	exploit	exploit	VBP
cord-328460-thx9zh11	9	12	the	the	DT
cord-328460-thx9zh11	9	13	polymerase	polymerase	NN
cord-328460-thx9zh11	9	14	chain	chain	NN
cord-328460-thx9zh11	9	15	reaction	reaction	NN
cord-328460-thx9zh11	9	16	(	(	-LRB-
cord-328460-thx9zh11	9	17	PCR	PCR	NNP
cord-328460-thx9zh11	9	18	)	)	-RRB-
cord-328460-thx9zh11	9	19	amplification	amplification	NN
cord-328460-thx9zh11	9	20	method	method	NN
cord-328460-thx9zh11	9	21	,	,	,
cord-328460-thx9zh11	9	22	which	which	WDT
cord-328460-thx9zh11	9	23	requires	require	VBZ
cord-328460-thx9zh11	9	24	repeated	repeat	VBN
cord-328460-thx9zh11	9	25	cycles	cycle	NNS
cord-328460-thx9zh11	9	26	of	of	IN
cord-328460-thx9zh11	9	27	three	three	CD
cord-328460-thx9zh11	9	28	or	or	CC
cord-328460-thx9zh11	9	29	two	two	CD
cord-328460-thx9zh11	9	30	temperature	temperature	NN
cord-328460-thx9zh11	9	31	-	-	HYPH
cord-328460-thx9zh11	9	32	dependent	dependent	JJ
cord-328460-thx9zh11	9	33	steps	step	NNS
cord-328460-thx9zh11	9	34	during	during	IN
cord-328460-thx9zh11	9	35	the	the	DT
cord-328460-thx9zh11	9	36	amplification	amplification	NN
cord-328460-thx9zh11	9	37	of	of	IN
cord-328460-thx9zh11	9	38	the	the	DT
cord-328460-thx9zh11	9	39	nucleic	nucleic	JJ
cord-328460-thx9zh11	9	40	acid	acid	NN
cord-328460-thx9zh11	9	41	target	target	NN
cord-328460-thx9zh11	9	42	sequence	sequence	NN
cord-328460-thx9zh11	9	43	.	.	.
cord-328460-thx9zh11	10	1	In	in	IN
cord-328460-thx9zh11	10	2	contrast	contrast	NN
cord-328460-thx9zh11	10	3	,	,	,
cord-328460-thx9zh11	10	4	low	low	JJ
cord-328460-thx9zh11	10	5	temperature	temperature	NN
cord-328460-thx9zh11	10	6	isothermal	isothermal	JJ
cord-328460-thx9zh11	10	7	amplification	amplification	NN
cord-328460-thx9zh11	10	8	methods	method	NNS
cord-328460-thx9zh11	10	9	have	have	VBP
cord-328460-thx9zh11	10	10	no	no	DT
cord-328460-thx9zh11	10	11	need	need	NN
cord-328460-thx9zh11	10	12	for	for	IN
cord-328460-thx9zh11	10	13	thermal	thermal	JJ
cord-328460-thx9zh11	10	14	cycling	cycling	NN
cord-328460-thx9zh11	10	15	thus	thus	RB
cord-328460-thx9zh11	10	16	requiring	require	VBG
cord-328460-thx9zh11	10	17	simplified	simplify	VBN
cord-328460-thx9zh11	10	18	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	10	19	device	device	NN
cord-328460-thx9zh11	10	20	features	feature	NNS
cord-328460-thx9zh11	10	21	.	.	.
cord-328460-thx9zh11	11	1	Here	here	RB
cord-328460-thx9zh11	11	2	,	,	,
cord-328460-thx9zh11	11	3	the	the	DT
cord-328460-thx9zh11	11	4	use	use	NN
cord-328460-thx9zh11	11	5	of	of	IN
cord-328460-thx9zh11	11	6	miniaturized	miniaturized	JJ
cord-328460-thx9zh11	11	7	analysis	analysis	NN
cord-328460-thx9zh11	11	8	systems	system	NNS
cord-328460-thx9zh11	11	9	using	use	VBG
cord-328460-thx9zh11	11	10	isothermal	isothermal	JJ
cord-328460-thx9zh11	11	11	amplification	amplification	NN
cord-328460-thx9zh11	11	12	reactions	reaction	NNS
cord-328460-thx9zh11	11	13	for	for	IN
cord-328460-thx9zh11	11	14	the	the	DT
cord-328460-thx9zh11	11	15	nucleic	nucleic	JJ
cord-328460-thx9zh11	11	16	acid	acid	NN
cord-328460-thx9zh11	11	17	amplification	amplification	NN
cord-328460-thx9zh11	11	18	will	will	MD
cord-328460-thx9zh11	11	19	be	be	VB
cord-328460-thx9zh11	11	20	discussed	discuss	VBN
cord-328460-thx9zh11	11	21	.	.	.
cord-328460-thx9zh11	12	1	A	a	DT
cord-328460-thx9zh11	12	2	great	great	JJ
cord-328460-thx9zh11	12	3	effort	effort	NN
cord-328460-thx9zh11	12	4	has	have	VBZ
cord-328460-thx9zh11	12	5	been	be	VBN
cord-328460-thx9zh11	12	6	devoted	devote	VBN
cord-328460-thx9zh11	12	7	,	,	,
cord-328460-thx9zh11	12	8	during	during	IN
cord-328460-thx9zh11	12	9	the	the	DT
cord-328460-thx9zh11	12	10	past	past	JJ
cord-328460-thx9zh11	12	11	decade	decade	NN
cord-328460-thx9zh11	12	12	,	,	,
cord-328460-thx9zh11	12	13	to	to	IN
cord-328460-thx9zh11	12	14	the	the	DT
cord-328460-thx9zh11	12	15	development	development	NN
cord-328460-thx9zh11	12	16	of	of	IN
cord-328460-thx9zh11	12	17	new	new	JJ
cord-328460-thx9zh11	12	18	devices	device	NNS
cord-328460-thx9zh11	12	19	for	for	IN
cord-328460-thx9zh11	12	20	specific	specific	JJ
cord-328460-thx9zh11	12	21	DNA	dna	NN
cord-328460-thx9zh11	12	22	sequences	sequence	NNS
cord-328460-thx9zh11	12	23	detection	detection	NN
cord-328460-thx9zh11	12	24	,	,	,
cord-328460-thx9zh11	12	25	due	due	IN
cord-328460-thx9zh11	12	26	to	to	IN
cord-328460-thx9zh11	12	27	the	the	DT
cord-328460-thx9zh11	12	28	relevance	relevance	NN
cord-328460-thx9zh11	12	29	of	of	IN
cord-328460-thx9zh11	12	30	nucleic	nucleic	JJ
cord-328460-thx9zh11	12	31	acid	acid	NN
cord-328460-thx9zh11	12	32	analysis	analysis	NN
cord-328460-thx9zh11	12	33	in	in	IN
cord-328460-thx9zh11	12	34	many	many	JJ
cord-328460-thx9zh11	12	35	areas	area	NNS
cord-328460-thx9zh11	12	36	,	,	,
cord-328460-thx9zh11	12	37	including	include	VBG
cord-328460-thx9zh11	12	38	clinical	clinical	JJ
cord-328460-thx9zh11	12	39	diagnostics	diagnostic	NNS
cord-328460-thx9zh11	12	40	[	[	-LRB-
cord-328460-thx9zh11	12	41	1	1	CD
cord-328460-thx9zh11	12	42	]	]	-RRB-
cord-328460-thx9zh11	12	43	,	,	,
cord-328460-thx9zh11	12	44	environmental	environmental	JJ
cord-328460-thx9zh11	12	45	monitoring	monitoring	NN
cord-328460-thx9zh11	12	46	and	and	CC
cord-328460-thx9zh11	12	47	food	food	NN
cord-328460-thx9zh11	12	48	-	-	HYPH
cord-328460-thx9zh11	12	49	quality	quality	NN
cord-328460-thx9zh11	12	50	control	control	NN
cord-328460-thx9zh11	12	51	[	[	-LRB-
cord-328460-thx9zh11	12	52	2	2	CD
cord-328460-thx9zh11	12	53	,	,	,
cord-328460-thx9zh11	12	54	3	3	CD
cord-328460-thx9zh11	12	55	]	]	-RRB-
cord-328460-thx9zh11	12	56	.	.	.
cord-328460-thx9zh11	13	1	However	however	RB
cord-328460-thx9zh11	13	2	,	,	,
cord-328460-thx9zh11	13	3	diagnostic	diagnostic	JJ
cord-328460-thx9zh11	13	4	tools	tool	NNS
cord-328460-thx9zh11	13	5	used	use	VBN
cord-328460-thx9zh11	13	6	to	to	TO
cord-328460-thx9zh11	13	7	detect	detect	VB
cord-328460-thx9zh11	13	8	nucleic	nucleic	JJ
cord-328460-thx9zh11	13	9	acids	acid	NNS
cord-328460-thx9zh11	13	10	need	need	VBP
cord-328460-thx9zh11	13	11	to	to	TO
cord-328460-thx9zh11	13	12	meet	meet	VB
cord-328460-thx9zh11	13	13	specific	specific	JJ
cord-328460-thx9zh11	13	14	requirements	requirement	NNS
cord-328460-thx9zh11	13	15	in	in	IN
cord-328460-thx9zh11	13	16	terms	term	NNS
cord-328460-thx9zh11	13	17	of	of	IN
cord-328460-thx9zh11	13	18	sensitivity	sensitivity	NN
cord-328460-thx9zh11	13	19	,	,	,
cord-328460-thx9zh11	13	20	selectivity	selectivity	NN
cord-328460-thx9zh11	13	21	and	and	CC
cord-328460-thx9zh11	13	22	high	high	JJ
cord-328460-thx9zh11	13	23	-	-	HYPH
cord-328460-thx9zh11	13	24	throughput	throughput	NN
cord-328460-thx9zh11	13	25	in	in	IN
cord-328460-thx9zh11	13	26	order	order	NN
cord-328460-thx9zh11	13	27	to	to	TO
cord-328460-thx9zh11	13	28	widen	widen	VB
cord-328460-thx9zh11	13	29	their	-PRON-	PRP$
cord-328460-thx9zh11	13	30	applicability	applicability	NN
cord-328460-thx9zh11	13	31	and	and	CC
cord-328460-thx9zh11	13	32	to	to	TO
cord-328460-thx9zh11	13	33	minimize	minimize	VB
cord-328460-thx9zh11	13	34	the	the	DT
cord-328460-thx9zh11	13	35	cost	cost	NN
cord-328460-thx9zh11	13	36	of	of	IN
cord-328460-thx9zh11	13	37	the	the	DT
cord-328460-thx9zh11	13	38	whole	whole	JJ
cord-328460-thx9zh11	13	39	detection	detection	NN
cord-328460-thx9zh11	13	40	assay	assay	NN
cord-328460-thx9zh11	13	41	[	[	-LRB-
cord-328460-thx9zh11	13	42	4	4	CD
cord-328460-thx9zh11	13	43	]	]	-RRB-
cord-328460-thx9zh11	13	44	.	.	.
cord-328460-thx9zh11	14	1	Often	often	RB
cord-328460-thx9zh11	14	2	,	,	,
cord-328460-thx9zh11	14	3	a	a	DT
cord-328460-thx9zh11	14	4	limited	limited	JJ
cord-328460-thx9zh11	14	5	amount	amount	NN
cord-328460-thx9zh11	14	6	of	of	IN
cord-328460-thx9zh11	14	7	DNA	dna	NN
cord-328460-thx9zh11	14	8	is	be	VBZ
cord-328460-thx9zh11	14	9	available	available	JJ
cord-328460-thx9zh11	14	10	,	,	,
cord-328460-thx9zh11	14	11	particularly	particularly	RB
cord-328460-thx9zh11	14	12	when	when	WRB
cord-328460-thx9zh11	14	13	biological	biological	JJ
cord-328460-thx9zh11	14	14	material	material	NN
cord-328460-thx9zh11	14	15	from	from	IN
cord-328460-thx9zh11	14	16	human	human	JJ
cord-328460-thx9zh11	14	17	samples	sample	NNS
cord-328460-thx9zh11	14	18	are	be	VBP
cord-328460-thx9zh11	14	19	going	go	VBG
cord-328460-thx9zh11	14	20	to	to	TO
cord-328460-thx9zh11	14	21	be	be	VB
cord-328460-thx9zh11	14	22	analyzed	analyze	VBN
cord-328460-thx9zh11	14	23	.	.	.
cord-328460-thx9zh11	15	1	This	this	DT
cord-328460-thx9zh11	15	2	is	be	VBZ
cord-328460-thx9zh11	15	3	true	true	JJ
cord-328460-thx9zh11	15	4	when	when	WRB
cord-328460-thx9zh11	15	5	,	,	,
cord-328460-thx9zh11	15	6	for	for	IN
cord-328460-thx9zh11	15	7	instance	instance	NN
cord-328460-thx9zh11	15	8	,	,	,
cord-328460-thx9zh11	15	9	circulating	circulate	VBG
cord-328460-thx9zh11	15	10	cell	cell	NN
cord-328460-thx9zh11	15	11	-	-	HYPH
cord-328460-thx9zh11	15	12	free	free	JJ
cord-328460-thx9zh11	15	13	fetal	fetal	JJ
cord-328460-thx9zh11	15	14	DNA	dna	NN
cord-328460-thx9zh11	15	15	in	in	IN
cord-328460-thx9zh11	15	16	maternal	maternal	JJ
cord-328460-thx9zh11	15	17	blood	blood	NN
cord-328460-thx9zh11	15	18	or	or	CC
cord-328460-thx9zh11	15	19	mutated	mutate	VBN
cord-328460-thx9zh11	15	20	DNA	dna	NN
cord-328460-thx9zh11	15	21	in	in	IN
cord-328460-thx9zh11	15	22	rare	rare	JJ
cord-328460-thx9zh11	15	23	circulating	circulate	VBG
cord-328460-thx9zh11	15	24	tumor	tumor	NN
cord-328460-thx9zh11	15	25	cells	cell	NNS
cord-328460-thx9zh11	15	26	are	be	VBP
cord-328460-thx9zh11	15	27	going	go	VBG
cord-328460-thx9zh11	15	28	to	to	TO
cord-328460-thx9zh11	15	29	be	be	VB
cord-328460-thx9zh11	15	30	considered	consider	VBN
cord-328460-thx9zh11	15	31	.	.	.
cord-328460-thx9zh11	16	1	Similar	similar	JJ
cord-328460-thx9zh11	16	2	limitations	limitation	NNS
cord-328460-thx9zh11	16	3	are	be	VBP
cord-328460-thx9zh11	16	4	also	also	RB
cord-328460-thx9zh11	16	5	found	find	VBN
cord-328460-thx9zh11	16	6	in	in	IN
cord-328460-thx9zh11	16	7	forensic	forensic	JJ
cord-328460-thx9zh11	16	8	applications	application	NNS
cord-328460-thx9zh11	16	9	due	due	JJ
cord-328460-thx9zh11	16	10	to	to	IN
cord-328460-thx9zh11	16	11	the	the	DT
cord-328460-thx9zh11	16	12	small	small	JJ
cord-328460-thx9zh11	16	13	amount	amount	NN
cord-328460-thx9zh11	16	14	of	of	IN
cord-328460-thx9zh11	16	15	biological	biological	JJ
cord-328460-thx9zh11	16	16	material	material	NN
cord-328460-thx9zh11	16	17	that	that	WDT
cord-328460-thx9zh11	16	18	may	may	MD
cord-328460-thx9zh11	16	19	be	be	VB
cord-328460-thx9zh11	16	20	recovered	recover	VBN
cord-328460-thx9zh11	16	21	from	from	IN
cord-328460-thx9zh11	16	22	some	some	DT
cord-328460-thx9zh11	16	23	crime	crime	NN
cord-328460-thx9zh11	16	24	scene	scene	NN
cord-328460-thx9zh11	16	25	samples	sample	NNS
cord-328460-thx9zh11	16	26	.	.	.
cord-328460-thx9zh11	17	1	In	in	IN
cord-328460-thx9zh11	17	2	this	this	DT
cord-328460-thx9zh11	17	3	perspective	perspective	NN
cord-328460-thx9zh11	17	4	,	,	,
cord-328460-thx9zh11	17	5	efforts	effort	NNS
cord-328460-thx9zh11	17	6	have	have	VBP
cord-328460-thx9zh11	17	7	been	be	VBN
cord-328460-thx9zh11	17	8	paid	pay	VBN
cord-328460-thx9zh11	17	9	in	in	IN
cord-328460-thx9zh11	17	10	developing	develop	VBG
cord-328460-thx9zh11	17	11	even	even	RB
cord-328460-thx9zh11	17	12	more	more	RBR
cord-328460-thx9zh11	17	13	sensitive	sensitive	JJ
cord-328460-thx9zh11	17	14	methods	method	NNS
cord-328460-thx9zh11	17	15	for	for	IN
cord-328460-thx9zh11	17	16	nucleic	nucleic	JJ
cord-328460-thx9zh11	17	17	acid	acid	NN
cord-328460-thx9zh11	17	18	detection	detection	NN
cord-328460-thx9zh11	17	19	,	,	,
cord-328460-thx9zh11	17	20	capable	capable	JJ
cord-328460-thx9zh11	17	21	of	of	IN
cord-328460-thx9zh11	17	22	directly	directly	RB
cord-328460-thx9zh11	17	23	transducing	transduce	VBG
cord-328460-thx9zh11	17	24	the	the	DT
cord-328460-thx9zh11	17	25	hybridization	hybridization	NN
cord-328460-thx9zh11	17	26	events	event	NNS
cord-328460-thx9zh11	17	27	from	from	IN
cord-328460-thx9zh11	17	28	limited	limited	JJ
cord-328460-thx9zh11	17	29	biological	biological	JJ
cord-328460-thx9zh11	17	30	samples	sample	NNS
cord-328460-thx9zh11	17	31	[	[	-LRB-
cord-328460-thx9zh11	17	32	4	4	CD
cord-328460-thx9zh11	17	33	]	]	-RRB-
cord-328460-thx9zh11	17	34	[	[	-LRB-
cord-328460-thx9zh11	17	35	5	5	CD
cord-328460-thx9zh11	17	36	]	]	-RRB-
cord-328460-thx9zh11	17	37	[	[	-LRB-
cord-328460-thx9zh11	17	38	6	6	CD
cord-328460-thx9zh11	17	39	]	]	-RRB-
cord-328460-thx9zh11	17	40	.	.	.
cord-328460-thx9zh11	18	1	It	-PRON-	PRP
cord-328460-thx9zh11	18	2	is	be	VBZ
cord-328460-thx9zh11	18	3	for	for	IN
cord-328460-thx9zh11	18	4	this	this	DT
cord-328460-thx9zh11	18	5	reason	reason	NN
cord-328460-thx9zh11	18	6	that	that	IN
cord-328460-thx9zh11	18	7	a	a	DT
cord-328460-thx9zh11	18	8	key	key	JJ
cord-328460-thx9zh11	18	9	step	step	NN
cord-328460-thx9zh11	18	10	in	in	IN
cord-328460-thx9zh11	18	11	nucleic	nucleic	JJ
cord-328460-thx9zh11	18	12	acid	acid	NN
cord-328460-thx9zh11	18	13	detection	detection	NN
cord-328460-thx9zh11	18	14	protocols	protocol	NNS
cord-328460-thx9zh11	18	15	is	be	VBZ
cord-328460-thx9zh11	18	16	the	the	DT
cord-328460-thx9zh11	18	17	amplification	amplification	NN
cord-328460-thx9zh11	18	18	of	of	IN
cord-328460-thx9zh11	18	19	the	the	DT
cord-328460-thx9zh11	18	20	target	target	NN
cord-328460-thx9zh11	18	21	sequence	sequence	NN
cord-328460-thx9zh11	18	22	.	.	.
cord-328460-thx9zh11	19	1	The	the	DT
cord-328460-thx9zh11	19	2	amplification	amplification	NN
cord-328460-thx9zh11	19	3	generates	generate	VBZ
cord-328460-thx9zh11	19	4	a	a	DT
cord-328460-thx9zh11	19	5	large	large	JJ
cord-328460-thx9zh11	19	6	number	number	NN
cord-328460-thx9zh11	19	7	of	of	IN
cord-328460-thx9zh11	19	8	target	target	NN
cord-328460-thx9zh11	19	9	copies	copy	NNS
cord-328460-thx9zh11	19	10	,	,	,
cord-328460-thx9zh11	19	11	greatly	greatly	RB
cord-328460-thx9zh11	19	12	enhancing	enhance	VBG
cord-328460-thx9zh11	19	13	the	the	DT
cord-328460-thx9zh11	19	14	assay	assay	NN
cord-328460-thx9zh11	19	15	sensitivity	sensitivity	NN
cord-328460-thx9zh11	19	16	[	[	-LRB-
cord-328460-thx9zh11	19	17	7	7	CD
cord-328460-thx9zh11	19	18	]	]	-RRB-
cord-328460-thx9zh11	19	19	.	.	.
cord-328460-thx9zh11	20	1	The	the	DT
cord-328460-thx9zh11	20	2	most	most	RBS
cord-328460-thx9zh11	20	3	widely	widely	RB
cord-328460-thx9zh11	20	4	adopted	adopt	VBN
cord-328460-thx9zh11	20	5	amplification	amplification	NN
cord-328460-thx9zh11	20	6	procedures	procedure	NNS
cord-328460-thx9zh11	20	7	exploit	exploit	VBP
cord-328460-thx9zh11	20	8	the	the	DT
cord-328460-thx9zh11	20	9	polymerase	polymerase	NN
cord-328460-thx9zh11	20	10	chain	chain	NN
cord-328460-thx9zh11	20	11	reaction	reaction	NN
cord-328460-thx9zh11	20	12	(	(	-LRB-
cord-328460-thx9zh11	20	13	PCR	PCR	NNP
cord-328460-thx9zh11	20	14	)	)	-RRB-
cord-328460-thx9zh11	20	15	based	base	VBN
cord-328460-thx9zh11	20	16	on	on	IN
cord-328460-thx9zh11	20	17	a	a	DT
cord-328460-thx9zh11	20	18	polymerase	polymerase	NN
cord-328460-thx9zh11	20	19	activity	activity	NN
cord-328460-thx9zh11	20	20	for	for	IN
cord-328460-thx9zh11	20	21	primer	primer	NN
cord-328460-thx9zh11	20	22	-	-	HYPH
cord-328460-thx9zh11	20	23	directed	direct	VBN
cord-328460-thx9zh11	20	24	target	target	NN
cord-328460-thx9zh11	20	25	amplification	amplification	NN
cord-328460-thx9zh11	20	26	.	.	.
cord-328460-thx9zh11	21	1	The	the	DT
cord-328460-thx9zh11	21	2	success	success	NN
cord-328460-thx9zh11	21	3	of	of	IN
cord-328460-thx9zh11	21	4	PCR	PCR	NNP
cord-328460-thx9zh11	21	5	amplification	amplification	NN
cord-328460-thx9zh11	21	6	is	be	VBZ
cord-328460-thx9zh11	21	7	related	relate	VBN
cord-328460-thx9zh11	21	8	to	to	IN
cord-328460-thx9zh11	21	9	its	-PRON-	PRP$
cord-328460-thx9zh11	21	10	simplicity	simplicity	NN
cord-328460-thx9zh11	21	11	and	and	CC
cord-328460-thx9zh11	21	12	its	-PRON-	PRP$
cord-328460-thx9zh11	21	13	cost	cost	NN
cord-328460-thx9zh11	21	14	-	-	HYPH
cord-328460-thx9zh11	21	15	effectiveness	effectiveness	NN
cord-328460-thx9zh11	21	16	.	.	.
cord-328460-thx9zh11	22	1	Moreover	moreover	RB
cord-328460-thx9zh11	22	2	,	,	,
cord-328460-thx9zh11	22	3	it	-PRON-	PRP
cord-328460-thx9zh11	22	4	has	have	VBZ
cord-328460-thx9zh11	22	5	the	the	DT
cord-328460-thx9zh11	22	6	potential	potential	NN
cord-328460-thx9zh11	22	7	for	for	IN
cord-328460-thx9zh11	22	8	a	a	DT
cord-328460-thx9zh11	22	9	single	single	JJ
cord-328460-thx9zh11	22	10	DNA	dna	NN
cord-328460-thx9zh11	22	11	molecule	molecule	NN
cord-328460-thx9zh11	22	12	amplification	amplification	NN
cord-328460-thx9zh11	22	13	(	(	-LRB-
cord-328460-thx9zh11	22	14	i.e.	i.e.	FW
cord-328460-thx9zh11	22	15	,	,	,
cord-328460-thx9zh11	22	16	single	single	JJ
cord-328460-thx9zh11	22	17	-	-	HYPH
cord-328460-thx9zh11	22	18	molecule	molecule	NN
cord-328460-thx9zh11	22	19	PCR	PCR	NNP
cord-328460-thx9zh11	22	20	)	)	-RRB-
cord-328460-thx9zh11	22	21	[	[	-LRB-
cord-328460-thx9zh11	22	22	8	8	CD
cord-328460-thx9zh11	22	23	,	,	,
cord-328460-thx9zh11	22	24	9	9	CD
cord-328460-thx9zh11	22	25	]	]	-RRB-
cord-328460-thx9zh11	22	26	due	due	IN
cord-328460-thx9zh11	22	27	to	to	IN
cord-328460-thx9zh11	22	28	its	-PRON-	PRP$
cord-328460-thx9zh11	22	29	highly	highly	RB
cord-328460-thx9zh11	22	30	efficient	efficient	JJ
cord-328460-thx9zh11	22	31	exponential	exponential	JJ
cord-328460-thx9zh11	22	32	process	process	NN
cord-328460-thx9zh11	22	33	.	.	.
cord-328460-thx9zh11	23	1	However	however	RB
cord-328460-thx9zh11	23	2	,	,	,
cord-328460-thx9zh11	23	3	despite	despite	IN
cord-328460-thx9zh11	23	4	its	-PRON-	PRP$
cord-328460-thx9zh11	23	5	many	many	JJ
cord-328460-thx9zh11	23	6	advantages	advantage	NNS
cord-328460-thx9zh11	23	7	,	,	,
cord-328460-thx9zh11	23	8	PCR	PCR	NNP
cord-328460-thx9zh11	23	9	suffers	suffer	VBZ
cord-328460-thx9zh11	23	10	from	from	IN
cord-328460-thx9zh11	23	11	drawbacks	drawback	NNS
cord-328460-thx9zh11	23	12	which	which	WDT
cord-328460-thx9zh11	23	13	have	have	VBP
cord-328460-thx9zh11	23	14	led	lead	VBN
cord-328460-thx9zh11	23	15	to	to	IN
cord-328460-thx9zh11	23	16	the	the	DT
cord-328460-thx9zh11	23	17	development	development	NN
cord-328460-thx9zh11	23	18	of	of	IN
cord-328460-thx9zh11	23	19	alternative	alternative	JJ
cord-328460-thx9zh11	23	20	amplification	amplification	NN
cord-328460-thx9zh11	23	21	methods	method	NNS
cord-328460-thx9zh11	23	22	[	[	-LRB-
cord-328460-thx9zh11	23	23	7	7	CD
cord-328460-thx9zh11	23	24	,	,	,
cord-328460-thx9zh11	23	25	10	10	CD
cord-328460-thx9zh11	23	26	]	]	-RRB-
cord-328460-thx9zh11	23	27	.	.	.
cord-328460-thx9zh11	24	1	Among	among	IN
cord-328460-thx9zh11	24	2	them	-PRON-	PRP
cord-328460-thx9zh11	24	3	,	,	,
cord-328460-thx9zh11	24	4	the	the	DT
cord-328460-thx9zh11	24	5	time	time	NN
cord-328460-thx9zh11	24	6	consuming	consume	VBG
cord-328460-thx9zh11	24	7	thermal	thermal	JJ
cord-328460-thx9zh11	24	8	cycling	cycling	NN
cord-328460-thx9zh11	24	9	between	between	IN
cord-328460-thx9zh11	24	10	different	different	JJ
cord-328460-thx9zh11	24	11	temperatures	temperature	NNS
cord-328460-thx9zh11	24	12	needed	need	VBN
cord-328460-thx9zh11	24	13	to	to	TO
cord-328460-thx9zh11	24	14	obtain	obtain	VB
cord-328460-thx9zh11	24	15	the	the	DT
cord-328460-thx9zh11	24	16	target	target	NN
cord-328460-thx9zh11	24	17	sequence	sequence	NN
cord-328460-thx9zh11	24	18	amplification	amplification	NN
cord-328460-thx9zh11	24	19	.	.	.
cord-328460-thx9zh11	25	1	When	when	WRB
cord-328460-thx9zh11	25	2	compared	compare	VBN
cord-328460-thx9zh11	25	3	to	to	IN
cord-328460-thx9zh11	25	4	PCR	PCR	NNP
cord-328460-thx9zh11	25	5	methods	method	NNS
cord-328460-thx9zh11	25	6	,	,	,
cord-328460-thx9zh11	25	7	isothermal	isothermal	JJ
cord-328460-thx9zh11	25	8	amplification	amplification	NN
cord-328460-thx9zh11	25	9	methods	method	NNS
cord-328460-thx9zh11	25	10	,	,	,
cord-328460-thx9zh11	25	11	not	not	RB
cord-328460-thx9zh11	25	12	requiring	require	VBG
cord-328460-thx9zh11	25	13	any	any	DT
cord-328460-thx9zh11	25	14	thermal	thermal	JJ
cord-328460-thx9zh11	25	15	cycling	cycling	NN
cord-328460-thx9zh11	25	16	,	,	,
cord-328460-thx9zh11	25	17	are	be	VBP
cord-328460-thx9zh11	25	18	easier	easy	JJR
cord-328460-thx9zh11	25	19	to	to	TO
cord-328460-thx9zh11	25	20	operate	operate	VB
cord-328460-thx9zh11	25	21	and	and	CC
cord-328460-thx9zh11	25	22	require	require	VB
cord-328460-thx9zh11	25	23	less	less	JJR
cord-328460-thx9zh11	25	24	energy	energy	NN
cord-328460-thx9zh11	25	25	than	than	IN
cord-328460-thx9zh11	25	26	PCR	PCR	NNP
cord-328460-thx9zh11	25	27	methods	method	NNS
cord-328460-thx9zh11	25	28	that	that	WDT
cord-328460-thx9zh11	25	29	instead	instead	RB
cord-328460-thx9zh11	25	30	require	require	VBP
cord-328460-thx9zh11	25	31	rapid	rapid	JJ
cord-328460-thx9zh11	25	32	heating	heating	NN
cord-328460-thx9zh11	25	33	and	and	CC
cord-328460-thx9zh11	25	34	cooling	cooling	NN
cord-328460-thx9zh11	25	35	steps	step	NNS
cord-328460-thx9zh11	25	36	.	.	.
cord-328460-thx9zh11	26	1	These	these	DT
cord-328460-thx9zh11	26	2	features	feature	NNS
cord-328460-thx9zh11	26	3	greatly	greatly	RB
cord-328460-thx9zh11	26	4	simplify	simplify	VBP
cord-328460-thx9zh11	26	5	the	the	DT
cord-328460-thx9zh11	26	6	isothermal	isothermal	JJ
cord-328460-thx9zh11	26	7	amplification	amplification	NN
cord-328460-thx9zh11	26	8	implementation	implementation	NN
cord-328460-thx9zh11	26	9	in	in	IN
cord-328460-thx9zh11	26	10	point	point	NN
cord-328460-thx9zh11	26	11	-	-	HYPH
cord-328460-thx9zh11	26	12	of	of	IN
cord-328460-thx9zh11	26	13	-	-	HYPH
cord-328460-thx9zh11	26	14	care	care	NN
cord-328460-thx9zh11	26	15	diagnostic	diagnostic	JJ
cord-328460-thx9zh11	26	16	devices	device	NNS
cord-328460-thx9zh11	26	17	.	.	.
cord-328460-thx9zh11	27	1	In	in	IN
cord-328460-thx9zh11	27	2	addition	addition	NN
cord-328460-thx9zh11	27	3	,	,	,
cord-328460-thx9zh11	27	4	the	the	DT
cord-328460-thx9zh11	27	5	use	use	NN
cord-328460-thx9zh11	27	6	of	of	IN
cord-328460-thx9zh11	27	7	fully	fully	RB
cord-328460-thx9zh11	27	8	enclosed	enclose	VBN
cord-328460-thx9zh11	27	9	microstructured	microstructured	JJ
cord-328460-thx9zh11	27	10	devices	device	NNS
cord-328460-thx9zh11	27	11	into	into	IN
cord-328460-thx9zh11	27	12	which	which	WDT
cord-328460-thx9zh11	27	13	performing	perform	VBG
cord-328460-thx9zh11	27	14	the	the	DT
cord-328460-thx9zh11	27	15	isothermal	isothermal	JJ
cord-328460-thx9zh11	27	16	amplification	amplification	NN
cord-328460-thx9zh11	27	17	reduces	reduce	VBZ
cord-328460-thx9zh11	27	18	the	the	DT
cord-328460-thx9zh11	27	19	risk	risk	NN
cord-328460-thx9zh11	27	20	of	of	IN
cord-328460-thx9zh11	27	21	sample	sample	NN
cord-328460-thx9zh11	27	22	contamination	contamination	NN
cord-328460-thx9zh11	27	23	and	and	CC
cord-328460-thx9zh11	27	24	implies	imply	VBZ
cord-328460-thx9zh11	27	25	low	low	JJ
cord-328460-thx9zh11	27	26	sample	sample	NN
cord-328460-thx9zh11	27	27	consumption	consumption	NN
cord-328460-thx9zh11	27	28	,	,	,
cord-328460-thx9zh11	27	29	multiplex	multiplex	JJ
cord-328460-thx9zh11	27	30	DNA	dna	NN
cord-328460-thx9zh11	27	31	analysis	analysis	NN
cord-328460-thx9zh11	27	32	,	,	,
cord-328460-thx9zh11	27	33	integration	integration	NN
cord-328460-thx9zh11	27	34	and	and	CC
cord-328460-thx9zh11	27	35	portable	portable	JJ
cord-328460-thx9zh11	27	36	devices	device	NNS
cord-328460-thx9zh11	27	37	realization	realization	NN
cord-328460-thx9zh11	27	38	.	.	.
cord-328460-thx9zh11	28	1	This	this	DT
cord-328460-thx9zh11	28	2	review	review	NN
cord-328460-thx9zh11	28	3	will	will	MD
cord-328460-thx9zh11	28	4	first	first	RB
cord-328460-thx9zh11	28	5	discuss	discuss	VB
cord-328460-thx9zh11	28	6	the	the	DT
cord-328460-thx9zh11	28	7	use	use	NN
cord-328460-thx9zh11	28	8	of	of	IN
cord-328460-thx9zh11	28	9	microfluidic	microfluidic	NN
cord-328460-thx9zh11	28	10	-	-	HYPH
cord-328460-thx9zh11	28	11	based	base	VBN
cord-328460-thx9zh11	28	12	systems	system	NNS
cord-328460-thx9zh11	28	13	for	for	IN
cord-328460-thx9zh11	28	14	nucleic	nucleic	JJ
cord-328460-thx9zh11	28	15	acid	acid	NN
cord-328460-thx9zh11	28	16	amplification	amplification	NN
cord-328460-thx9zh11	28	17	by	by	IN
cord-328460-thx9zh11	28	18	underlining	underline	VBG
cord-328460-thx9zh11	28	19	advantages	advantage	NNS
cord-328460-thx9zh11	28	20	coming	come	VBG
cord-328460-thx9zh11	28	21	from	from	IN
cord-328460-thx9zh11	28	22	the	the	DT
cord-328460-thx9zh11	28	23	rapidity	rapidity	NN
cord-328460-thx9zh11	28	24	of	of	IN
cord-328460-thx9zh11	28	25	the	the	DT
cord-328460-thx9zh11	28	26	process	process	NN
cord-328460-thx9zh11	28	27	and	and	CC
cord-328460-thx9zh11	28	28	the	the	DT
cord-328460-thx9zh11	28	29	low	low	JJ
cord-328460-thx9zh11	28	30	sample	sample	NN
cord-328460-thx9zh11	28	31	consumption	consumption	NN
cord-328460-thx9zh11	28	32	and	and	CC
cord-328460-thx9zh11	28	33	discussing	discuss	VBG
cord-328460-thx9zh11	28	34	drawbacks	drawback	NNS
cord-328460-thx9zh11	28	35	caused	cause	VBN
cord-328460-thx9zh11	28	36	by	by	IN
cord-328460-thx9zh11	28	37	surface	surface	NN
cord-328460-thx9zh11	28	38	-	-	HYPH
cord-328460-thx9zh11	28	39	dependent	dependent	JJ
cord-328460-thx9zh11	28	40	processes	process	NNS
cord-328460-thx9zh11	28	41	.	.	.
cord-328460-thx9zh11	29	1	Miniaturized	miniaturized	JJ
cord-328460-thx9zh11	29	2	isothermal	isothermal	JJ
cord-328460-thx9zh11	29	3	amplification	amplification	NN
cord-328460-thx9zh11	29	4	systems	system	NNS
cord-328460-thx9zh11	29	5	will	will	MD
cord-328460-thx9zh11	29	6	be	be	VB
cord-328460-thx9zh11	29	7	then	then	RB
cord-328460-thx9zh11	29	8	illustrated	illustrate	VBN
cord-328460-thx9zh11	29	9	including	include	VBG
cord-328460-thx9zh11	29	10	nucleic	nucleic	JJ
cord-328460-thx9zh11	29	11	acid	acid	NN
cord-328460-thx9zh11	29	12	sequence	sequence	NN
cord-328460-thx9zh11	29	13	-	-	HYPH
cord-328460-thx9zh11	29	14	based	base	VBN
cord-328460-thx9zh11	29	15	amplification	amplification	NN
cord-328460-thx9zh11	29	16	(	(	-LRB-
cord-328460-thx9zh11	29	17	NASBA	NASBA	NNP
cord-328460-thx9zh11	29	18	)	)	-RRB-
cord-328460-thx9zh11	29	19	,	,	,
cord-328460-thx9zh11	29	20	loop	loop	NN
cord-328460-thx9zh11	29	21	-	-	HYPH
cord-328460-thx9zh11	29	22	mediated	mediate	VBN
cord-328460-thx9zh11	29	23	isothermal	isothermal	JJ
cord-328460-thx9zh11	29	24	amplification	amplification	NN
cord-328460-thx9zh11	29	25	(	(	-LRB-
cord-328460-thx9zh11	29	26	LAMP	LAMP	NNP
cord-328460-thx9zh11	29	27	)	)	-RRB-
cord-328460-thx9zh11	29	28	,	,	,
cord-328460-thx9zh11	29	29	helicase	helicase	NN
cord-328460-thx9zh11	29	30	-	-	HYPH
cord-328460-thx9zh11	29	31	dependent	dependent	JJ
cord-328460-thx9zh11	29	32	amplification	amplification	NN
cord-328460-thx9zh11	29	33	(	(	-LRB-
cord-328460-thx9zh11	29	34	HDA	HDA	NNP
cord-328460-thx9zh11	29	35	)	)	-RRB-
cord-328460-thx9zh11	29	36	,	,	,
cord-328460-thx9zh11	29	37	rolling	roll	VBG
cord-328460-thx9zh11	29	38	circle	circle	NN
cord-328460-thx9zh11	29	39	amplification	amplification	NN
cord-328460-thx9zh11	29	40	(	(	-LRB-
cord-328460-thx9zh11	29	41	RCA	RCA	NNP
cord-328460-thx9zh11	29	42	)	)	-RRB-
cord-328460-thx9zh11	29	43	,	,	,
cord-328460-thx9zh11	29	44	multiple	multiple	JJ
cord-328460-thx9zh11	29	45	displacement	displacement	NN
cord-328460-thx9zh11	29	46	amplification	amplification	NN
cord-328460-thx9zh11	29	47	(	(	-LRB-
cord-328460-thx9zh11	29	48	MDA	MDA	NNP
cord-328460-thx9zh11	29	49	)	)	-RRB-
cord-328460-thx9zh11	29	50	and	and	CC
cord-328460-thx9zh11	29	51	recombinase	recombinase	NN
cord-328460-thx9zh11	29	52	polymerase	polymerase	NN
cord-328460-thx9zh11	29	53	amplification	amplification	NN
cord-328460-thx9zh11	29	54	(	(	-LRB-
cord-328460-thx9zh11	29	55	RPA	RPA	NNP
cord-328460-thx9zh11	29	56	)	)	-RRB-
cord-328460-thx9zh11	29	57	.	.	.
cord-328460-thx9zh11	30	1	Since	since	IN
cord-328460-thx9zh11	30	2	its	-PRON-	PRP$
cord-328460-thx9zh11	30	3	advent	advent	NN
cord-328460-thx9zh11	30	4	,	,	,
cord-328460-thx9zh11	30	5	microfluidic	microfluidic	NNP
cord-328460-thx9zh11	30	6	has	have	VBZ
cord-328460-thx9zh11	30	7	drawn	draw	VBN
cord-328460-thx9zh11	30	8	great	great	JJ
cord-328460-thx9zh11	30	9	attention	attention	NN
cord-328460-thx9zh11	30	10	for	for	IN
cord-328460-thx9zh11	30	11	the	the	DT
cord-328460-thx9zh11	30	12	capability	capability	NN
cord-328460-thx9zh11	30	13	of	of	IN
cord-328460-thx9zh11	30	14	miniaturizing	miniaturize	VBG
cord-328460-thx9zh11	30	15	typical	typical	JJ
cord-328460-thx9zh11	30	16	laboratory	laboratory	NN
cord-328460-thx9zh11	30	17	operations	operation	NNS
cord-328460-thx9zh11	30	18	by	by	IN
cord-328460-thx9zh11	30	19	using	use	VBG
cord-328460-thx9zh11	30	20	a	a	DT
cord-328460-thx9zh11	30	21	fraction	fraction	NN
cord-328460-thx9zh11	30	22	of	of	IN
cord-328460-thx9zh11	30	23	the	the	DT
cord-328460-thx9zh11	30	24	volume	volume	NN
cord-328460-thx9zh11	30	25	of	of	IN
cord-328460-thx9zh11	30	26	reagents	reagent	NNS
cord-328460-thx9zh11	30	27	in	in	IN
cord-328460-thx9zh11	30	28	significantly	significantly	RB
cord-328460-thx9zh11	30	29	less	less	JJR
cord-328460-thx9zh11	30	30	time	time	NN
cord-328460-thx9zh11	30	31	[	[	-LRB-
cord-328460-thx9zh11	30	32	11	11	CD
cord-328460-thx9zh11	30	33	]	]	-RRB-
cord-328460-thx9zh11	30	34	.	.	.
cord-328460-thx9zh11	31	1	Compared	compare	VBN
cord-328460-thx9zh11	31	2	with	with	IN
cord-328460-thx9zh11	31	3	the	the	DT
cord-328460-thx9zh11	31	4	macroscale	macroscale	NN
cord-328460-thx9zh11	31	5	,	,	,
cord-328460-thx9zh11	31	6	the	the	DT
cord-328460-thx9zh11	31	7	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	31	8	environment	environment	NN
cord-328460-thx9zh11	31	9	offers	offer	VBZ
cord-328460-thx9zh11	31	10	important	important	JJ
cord-328460-thx9zh11	31	11	advantages	advantage	NNS
cord-328460-thx9zh11	31	12	in	in	IN
cord-328460-thx9zh11	31	13	biochemical	biochemical	JJ
cord-328460-thx9zh11	31	14	detection	detection	NN
cord-328460-thx9zh11	31	15	,	,	,
cord-328460-thx9zh11	31	16	such	such	JJ
cord-328460-thx9zh11	31	17	as	as	IN
cord-328460-thx9zh11	31	18	shorter	short	JJR
cord-328460-thx9zh11	31	19	times	time	NNS
cord-328460-thx9zh11	31	20	for	for	IN
cord-328460-thx9zh11	31	21	the	the	DT
cord-328460-thx9zh11	31	22	analysis	analysis	NN
cord-328460-thx9zh11	31	23	,	,	,
cord-328460-thx9zh11	31	24	faster	fast	JJR
cord-328460-thx9zh11	31	25	mass	mass	NN
cord-328460-thx9zh11	31	26	and	and	CC
cord-328460-thx9zh11	31	27	thermal	thermal	JJ
cord-328460-thx9zh11	31	28	transfer	transfer	NN
cord-328460-thx9zh11	31	29	,	,	,
cord-328460-thx9zh11	31	30	reduced	reduce	VBN
cord-328460-thx9zh11	31	31	sample	sample	NN
cord-328460-thx9zh11	31	32	volumes	volume	NNS
cord-328460-thx9zh11	31	33	,	,	,
cord-328460-thx9zh11	31	34	and	and	CC
cord-328460-thx9zh11	31	35	potential	potential	NN
cord-328460-thx9zh11	31	36	for	for	IN
cord-328460-thx9zh11	31	37	automation	automation	NN
cord-328460-thx9zh11	31	38	and	and	CC
cord-328460-thx9zh11	31	39	integration	integration	NN
cord-328460-thx9zh11	31	40	.	.	.
cord-328460-thx9zh11	32	1	Moreover	moreover	RB
cord-328460-thx9zh11	32	2	,	,	,
cord-328460-thx9zh11	32	3	the	the	DT
cord-328460-thx9zh11	32	4	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	32	5	environment	environment	NN
cord-328460-thx9zh11	32	6	offers	offer	VBZ
cord-328460-thx9zh11	32	7	fundamentally	fundamentally	RB
cord-328460-thx9zh11	32	8	new	new	JJ
cord-328460-thx9zh11	32	9	capabilities	capability	NNS
cord-328460-thx9zh11	32	10	for	for	IN
cord-328460-thx9zh11	32	11	fluids	fluid	NNS
cord-328460-thx9zh11	32	12	control	control	NN
cord-328460-thx9zh11	32	13	.	.	.
cord-328460-thx9zh11	33	1	A	a	DT
cord-328460-thx9zh11	33	2	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	33	3	device	device	NN
cord-328460-thx9zh11	33	4	may	may	MD
cord-328460-thx9zh11	33	5	include	include	VB
cord-328460-thx9zh11	33	6	several	several	JJ
cord-328460-thx9zh11	33	7	components	component	NNS
cord-328460-thx9zh11	33	8	such	such	JJ
cord-328460-thx9zh11	33	9	as	as	IN
cord-328460-thx9zh11	33	10	valves	valve	NNS
cord-328460-thx9zh11	33	11	,	,	,
cord-328460-thx9zh11	33	12	mixers	mixer	NNS
cord-328460-thx9zh11	33	13	and	and	CC
cord-328460-thx9zh11	33	14	pumps	pump	NNS
cord-328460-thx9zh11	33	15	capable	capable	JJ
cord-328460-thx9zh11	33	16	of	of	IN
cord-328460-thx9zh11	33	17	controlling	control	VBG
cord-328460-thx9zh11	33	18	the	the	DT
cord-328460-thx9zh11	33	19	fluid	fluid	JJ
cord-328460-thx9zh11	33	20	flow	flow	NN
cord-328460-thx9zh11	33	21	within	within	IN
cord-328460-thx9zh11	33	22	the	the	DT
cord-328460-thx9zh11	33	23	device	device	NN
cord-328460-thx9zh11	33	24	and	and	CC
cord-328460-thx9zh11	33	25	other	other	JJ
cord-328460-thx9zh11	33	26	components	component	NNS
cord-328460-thx9zh11	33	27	useful	useful	JJ
cord-328460-thx9zh11	33	28	for	for	IN
cord-328460-thx9zh11	33	29	sample	sample	NN
cord-328460-thx9zh11	33	30	pre	pre	NNS
cord-328460-thx9zh11	33	31	-	-	NNS
cord-328460-thx9zh11	33	32	treatments	treatment	NNS
cord-328460-thx9zh11	33	33	or	or	CC
cord-328460-thx9zh11	33	34	detection	detection	NN
cord-328460-thx9zh11	33	35	.	.	.
cord-328460-thx9zh11	34	1	Liquids	liquid	NNS
cord-328460-thx9zh11	34	2	confined	confine	VBN
cord-328460-thx9zh11	34	3	into	into	IN
cord-328460-thx9zh11	34	4	the	the	DT
cord-328460-thx9zh11	34	5	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	34	6	environment	environment	NN
cord-328460-thx9zh11	34	7	experience	experience	NN
cord-328460-thx9zh11	34	8	an	an	DT
cord-328460-thx9zh11	34	9	interplay	interplay	NN
cord-328460-thx9zh11	34	10	of	of	IN
cord-328460-thx9zh11	34	11	multiple	multiple	JJ
cord-328460-thx9zh11	34	12	physical	physical	JJ
cord-328460-thx9zh11	34	13	effects	effect	NNS
cord-328460-thx9zh11	34	14	that	that	WDT
cord-328460-thx9zh11	34	15	lead	lead	VBP
cord-328460-thx9zh11	34	16	to	to	IN
cord-328460-thx9zh11	34	17	variants	variant	NNS
cord-328460-thx9zh11	34	18	of	of	IN
cord-328460-thx9zh11	34	19	physical	physical	JJ
cord-328460-thx9zh11	34	20	properties	property	NNS
cord-328460-thx9zh11	34	21	of	of	IN
cord-328460-thx9zh11	34	22	fluids	fluid	NNS
cord-328460-thx9zh11	34	23	moving	move	VBG
cord-328460-thx9zh11	34	24	in	in	IN
cord-328460-thx9zh11	34	25	large	large	JJ
cord-328460-thx9zh11	34	26	channels	channel	NNS
cord-328460-thx9zh11	34	27	.	.	.
cord-328460-thx9zh11	35	1	Microfluidics	Microfluidics	NNP
cord-328460-thx9zh11	35	2	has	have	VBZ
cord-328460-thx9zh11	35	3	been	be	VBN
cord-328460-thx9zh11	35	4	able	able	JJ
cord-328460-thx9zh11	35	5	to	to	TO
cord-328460-thx9zh11	35	6	exploit	exploit	VB
cord-328460-thx9zh11	35	7	differences	difference	NNS
cord-328460-thx9zh11	35	8	existing	exist	VBG
cord-328460-thx9zh11	35	9	between	between	IN
cord-328460-thx9zh11	35	10	fluids	fluid	NNS
cord-328460-thx9zh11	35	11	flowing	flow	VBG
cord-328460-thx9zh11	35	12	at	at	IN
cord-328460-thx9zh11	35	13	the	the	DT
cord-328460-thx9zh11	35	14	macroscale	macroscale	NN
cord-328460-thx9zh11	35	15	or	or	CC
cord-328460-thx9zh11	35	16	travelling	travel	VBG
cord-328460-thx9zh11	35	17	through	through	IN
cord-328460-thx9zh11	35	18	microchannels	microchannel	NNS
cord-328460-thx9zh11	35	19	,	,	,
cord-328460-thx9zh11	35	20	allowing	allow	VBG
cord-328460-thx9zh11	35	21	one	one	CD
cord-328460-thx9zh11	35	22	to	to	TO
cord-328460-thx9zh11	35	23	perform	perform	VB
cord-328460-thx9zh11	35	24	techniques	technique	NNS
cord-328460-thx9zh11	35	25	and	and	CC
cord-328460-thx9zh11	35	26	experiments	experiment	NNS
cord-328460-thx9zh11	35	27	which	which	WDT
cord-328460-thx9zh11	35	28	are	be	VBP
cord-328460-thx9zh11	35	29	impossible	impossible	JJ
cord-328460-thx9zh11	35	30	at	at	IN
cord-328460-thx9zh11	35	31	the	the	DT
cord-328460-thx9zh11	35	32	macroscale	macroscale	NN
cord-328460-thx9zh11	35	33	[	[	-LRB-
cord-328460-thx9zh11	35	34	12	12	CD
cord-328460-thx9zh11	35	35	]	]	-RRB-
cord-328460-thx9zh11	35	36	.	.	.
cord-328460-thx9zh11	36	1	In	in	IN
cord-328460-thx9zh11	36	2	the	the	DT
cord-328460-thx9zh11	36	3	typical	typical	JJ
cord-328460-thx9zh11	36	4	low	low	JJ
cord-328460-thx9zh11	36	5	Reynolds	Reynolds	NNP
cord-328460-thx9zh11	36	6	number	number	NN
cord-328460-thx9zh11	36	7	regime	regime	NN
cord-328460-thx9zh11	36	8	of	of	IN
cord-328460-thx9zh11	36	9	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	36	10	,	,	,
cord-328460-thx9zh11	36	11	viscous	viscous	JJ
cord-328460-thx9zh11	36	12	forces	force	NNS
cord-328460-thx9zh11	36	13	overwhelm	overwhelm	VBP
cord-328460-thx9zh11	36	14	inertial	inertial	JJ
cord-328460-thx9zh11	36	15	forces	force	NNS
cord-328460-thx9zh11	36	16	and	and	CC
cord-328460-thx9zh11	36	17	fluids	fluid	NNS
cord-328460-thx9zh11	36	18	adopt	adopt	VB
cord-328460-thx9zh11	36	19	a	a	DT
cord-328460-thx9zh11	36	20	laminar	laminar	JJ
cord-328460-thx9zh11	36	21	flow	flow	NN
cord-328460-thx9zh11	36	22	[	[	-LRB-
cord-328460-thx9zh11	36	23	13	13	CD
cord-328460-thx9zh11	36	24	,	,	,
cord-328460-thx9zh11	36	25	14	14	CD
cord-328460-thx9zh11	36	26	]	]	-RRB-
cord-328460-thx9zh11	36	27	.	.	.
cord-328460-thx9zh11	37	1	This	this	DT
cord-328460-thx9zh11	37	2	fact	fact	NN
cord-328460-thx9zh11	37	3	has	have	VBZ
cord-328460-thx9zh11	37	4	important	important	JJ
cord-328460-thx9zh11	37	5	implications	implication	NNS
cord-328460-thx9zh11	37	6	and	and	CC
cord-328460-thx9zh11	37	7	constitutes	constitute	VBZ
cord-328460-thx9zh11	37	8	the	the	DT
cord-328460-thx9zh11	37	9	basis	basis	NN
cord-328460-thx9zh11	37	10	of	of	IN
cord-328460-thx9zh11	37	11	many	many	JJ
cord-328460-thx9zh11	37	12	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	37	13	-	-	HYPH
cord-328460-thx9zh11	37	14	based	base	VBN
cord-328460-thx9zh11	37	15	technologies	technology	NNS
cord-328460-thx9zh11	37	16	.	.	.
cord-328460-thx9zh11	38	1	Fluid	fluid	JJ
cord-328460-thx9zh11	38	2	mixing	mixing	NN
cord-328460-thx9zh11	38	3	in	in	IN
cord-328460-thx9zh11	38	4	the	the	DT
cord-328460-thx9zh11	38	5	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	38	6	regime	regime	NN
cord-328460-thx9zh11	38	7	occurs	occur	VBZ
cord-328460-thx9zh11	38	8	through	through	IN
cord-328460-thx9zh11	38	9	the	the	DT
cord-328460-thx9zh11	38	10	diffusion	diffusion	NN
cord-328460-thx9zh11	38	11	,	,	,
cord-328460-thx9zh11	38	12	thus	thus	RB
cord-328460-thx9zh11	38	13	resulting	result	VBG
cord-328460-thx9zh11	38	14	in	in	IN
cord-328460-thx9zh11	38	15	prolonged	prolong	VBN
cord-328460-thx9zh11	38	16	mixing	mix	VBG
cord-328460-thx9zh11	38	17	times	time	NNS
cord-328460-thx9zh11	38	18	.	.	.
cord-328460-thx9zh11	39	1	Purely	purely	RB
cord-328460-thx9zh11	39	2	diffusive	diffusive	JJ
cord-328460-thx9zh11	39	3	mixing	mixing	NN
cord-328460-thx9zh11	39	4	can	can	MD
cord-328460-thx9zh11	39	5	be	be	VB
cord-328460-thx9zh11	39	6	desirable	desirable	JJ
cord-328460-thx9zh11	39	7	for	for	IN
cord-328460-thx9zh11	39	8	some	some	DT
cord-328460-thx9zh11	39	9	applications	application	NNS
cord-328460-thx9zh11	39	10	while	while	IN
cord-328460-thx9zh11	39	11	it	-PRON-	PRP
cord-328460-thx9zh11	39	12	is	be	VBZ
cord-328460-thx9zh11	39	13	to	to	TO
cord-328460-thx9zh11	39	14	be	be	VB
cord-328460-thx9zh11	39	15	avoided	avoid	VBN
cord-328460-thx9zh11	39	16	in	in	IN
cord-328460-thx9zh11	39	17	others	other	NNS
cord-328460-thx9zh11	39	18	.	.	.
cord-328460-thx9zh11	40	1	Thus	thus	RB
cord-328460-thx9zh11	40	2	,	,	,
cord-328460-thx9zh11	40	3	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	40	4	chemical	chemical	NN
cord-328460-thx9zh11	40	5	reactors	reactor	NNS
cord-328460-thx9zh11	40	6	require	require	VBP
cord-328460-thx9zh11	40	7	different	different	JJ
cord-328460-thx9zh11	40	8	approaches	approach	NNS
cord-328460-thx9zh11	40	9	in	in	IN
cord-328460-thx9zh11	40	10	order	order	NN
cord-328460-thx9zh11	40	11	to	to	TO
cord-328460-thx9zh11	40	12	bring	bring	VB
cord-328460-thx9zh11	40	13	fluids	fluid	NNS
cord-328460-thx9zh11	40	14	together	together	RB
cord-328460-thx9zh11	40	15	and	and	CC
cord-328460-thx9zh11	40	16	to	to	TO
cord-328460-thx9zh11	40	17	mix	mix	VB
cord-328460-thx9zh11	40	18	them	-PRON-	PRP
cord-328460-thx9zh11	40	19	rapidly	rapidly	RB
cord-328460-thx9zh11	40	20	.	.	.
cord-328460-thx9zh11	41	1	The	the	DT
cord-328460-thx9zh11	41	2	diffusion	diffusion	NN
cord-328460-thx9zh11	41	3	length	length	NN
cord-328460-thx9zh11	41	4	,	,	,
cord-328460-thx9zh11	41	5	and	and	CC
cord-328460-thx9zh11	41	6	hence	hence	RB
cord-328460-thx9zh11	41	7	,	,	,
cord-328460-thx9zh11	41	8	the	the	DT
cord-328460-thx9zh11	41	9	mixing	mix	VBG
cord-328460-thx9zh11	41	10	time	time	NN
cord-328460-thx9zh11	41	11	can	can	MD
cord-328460-thx9zh11	41	12	be	be	VB
cord-328460-thx9zh11	41	13	drastically	drastically	RB
cord-328460-thx9zh11	41	14	shortened	shorten	VBN
cord-328460-thx9zh11	41	15	by	by	IN
cord-328460-thx9zh11	41	16	applying	apply	VBG
cord-328460-thx9zh11	41	17	multiple	multiple	JJ
cord-328460-thx9zh11	41	18	laminar	laminar	JJ
cord-328460-thx9zh11	41	19	flow	flow	NN
cord-328460-thx9zh11	41	20	sheets	sheet	NNS
cord-328460-thx9zh11	41	21	so	so	IN
cord-328460-thx9zh11	41	22	that	that	IN
cord-328460-thx9zh11	41	23	millisecond	millisecond	NN
cord-328460-thx9zh11	41	24	time	time	NN
cord-328460-thx9zh11	41	25	resolutions	resolution	NNS
cord-328460-thx9zh11	41	26	can	can	MD
cord-328460-thx9zh11	41	27	be	be	VB
cord-328460-thx9zh11	41	28	reached	reach	VBN
cord-328460-thx9zh11	41	29	[	[	-LRB-
cord-328460-thx9zh11	41	30	15	15	CD
cord-328460-thx9zh11	41	31	,	,	,
cord-328460-thx9zh11	41	32	16	16	CD
cord-328460-thx9zh11	41	33	]	]	-RRB-
cord-328460-thx9zh11	41	34	.	.	.
cord-328460-thx9zh11	42	1	Passive	passive	JJ
cord-328460-thx9zh11	42	2	mixing	mix	VBG
cord-328460-thx9zh11	42	3	principles	principle	NNS
cord-328460-thx9zh11	42	4	are	be	VBP
cord-328460-thx9zh11	42	5	very	very	RB
cord-328460-thx9zh11	42	6	attractive	attractive	JJ
cord-328460-thx9zh11	42	7	because	because	IN
cord-328460-thx9zh11	42	8	they	-PRON-	PRP
cord-328460-thx9zh11	42	9	rely	rely	VBP
cord-328460-thx9zh11	42	10	on	on	IN
cord-328460-thx9zh11	42	11	diffusion	diffusion	NN
cord-328460-thx9zh11	42	12	or	or	CC
cord-328460-thx9zh11	42	13	chaotic	chaotic	JJ
cord-328460-thx9zh11	42	14	advection	advection	NN
cord-328460-thx9zh11	42	15	through	through	IN
cord-328460-thx9zh11	42	16	specially	specially	RB
cord-328460-thx9zh11	42	17	-	-	HYPH
cord-328460-thx9zh11	42	18	designed	design	VBN
cord-328460-thx9zh11	42	19	microchannels	microchannel	NNS
cord-328460-thx9zh11	42	20	.	.	.
cord-328460-thx9zh11	43	1	They	-PRON-	PRP
cord-328460-thx9zh11	43	2	can	can	MD
cord-328460-thx9zh11	43	3	also	also	RB
cord-328460-thx9zh11	43	4	operated	operate	VBN
cord-328460-thx9zh11	43	5	by	by	IN
cord-328460-thx9zh11	43	6	avoiding	avoid	VBG
cord-328460-thx9zh11	43	7	the	the	DT
cord-328460-thx9zh11	43	8	need	need	NN
cord-328460-thx9zh11	43	9	of	of	IN
cord-328460-thx9zh11	43	10	any	any	DT
cord-328460-thx9zh11	43	11	external	external	JJ
cord-328460-thx9zh11	43	12	energy	energy	NN
cord-328460-thx9zh11	43	13	source	source	NN
cord-328460-thx9zh11	43	14	.	.	.
cord-328460-thx9zh11	44	1	Active	active	JJ
cord-328460-thx9zh11	44	2	mixing	mixing	NN
cord-328460-thx9zh11	44	3	schemes	scheme	NNS
cord-328460-thx9zh11	44	4	,	,	,
cord-328460-thx9zh11	44	5	on	on	IN
cord-328460-thx9zh11	44	6	the	the	DT
cord-328460-thx9zh11	44	7	other	other	JJ
cord-328460-thx9zh11	44	8	hand	hand	NN
cord-328460-thx9zh11	44	9	,	,	,
cord-328460-thx9zh11	44	10	improve	improve	VB
cord-328460-thx9zh11	44	11	the	the	DT
cord-328460-thx9zh11	44	12	mixing	mix	VBG
cord-328460-thx9zh11	44	13	performance	performance	NN
cord-328460-thx9zh11	44	14	by	by	IN
cord-328460-thx9zh11	44	15	applying	apply	VBG
cord-328460-thx9zh11	44	16	external	external	JJ
cord-328460-thx9zh11	44	17	forces	force	NNS
cord-328460-thx9zh11	44	18	to	to	TO
cord-328460-thx9zh11	44	19	perturb	perturb	VB
cord-328460-thx9zh11	44	20	sample	sample	NN
cord-328460-thx9zh11	44	21	flows	flow	NNS
cord-328460-thx9zh11	44	22	and	and	CC
cord-328460-thx9zh11	44	23	consequently	consequently	RB
cord-328460-thx9zh11	44	24	to	to	TO
cord-328460-thx9zh11	44	25	accelerate	accelerate	VB
cord-328460-thx9zh11	44	26	the	the	DT
cord-328460-thx9zh11	44	27	diffusion	diffusion	NN
cord-328460-thx9zh11	44	28	processes	process	NNS
cord-328460-thx9zh11	44	29	.	.	.
cord-328460-thx9zh11	45	1	The	the	DT
cord-328460-thx9zh11	45	2	latter	latter	JJ
cord-328460-thx9zh11	45	3	configuration	configuration	NN
cord-328460-thx9zh11	45	4	often	often	RB
cord-328460-thx9zh11	45	5	leads	lead	VBZ
cord-328460-thx9zh11	45	6	to	to	IN
cord-328460-thx9zh11	45	7	a	a	DT
cord-328460-thx9zh11	45	8	complex	complex	JJ
cord-328460-thx9zh11	45	9	device	device	NN
cord-328460-thx9zh11	45	10	design	design	NN
cord-328460-thx9zh11	45	11	[	[	-LRB-
cord-328460-thx9zh11	45	12	17	17	CD
cord-328460-thx9zh11	45	13	]	]	-RRB-
cord-328460-thx9zh11	45	14	.	.	.
cord-328460-thx9zh11	46	1	Enhanced	enhanced	JJ
cord-328460-thx9zh11	46	2	sensitivity	sensitivity	NN
cord-328460-thx9zh11	46	3	can	can	MD
cord-328460-thx9zh11	46	4	sometimes	sometimes	RB
cord-328460-thx9zh11	46	5	be	be	VB
cord-328460-thx9zh11	46	6	achieved	achieve	VBN
cord-328460-thx9zh11	46	7	by	by	IN
cord-328460-thx9zh11	46	8	increasing	increase	VBG
cord-328460-thx9zh11	46	9	the	the	DT
cord-328460-thx9zh11	46	10	reaction	reaction	NN
cord-328460-thx9zh11	46	11	time	time	NN
cord-328460-thx9zh11	46	12	before	before	IN
cord-328460-thx9zh11	46	13	the	the	DT
cord-328460-thx9zh11	46	14	detection	detection	NN
cord-328460-thx9zh11	46	15	as	as	IN
cord-328460-thx9zh11	46	16	concerned	concern	VBN
cord-328460-thx9zh11	46	17	in	in	IN
cord-328460-thx9zh11	46	18	the	the	DT
cord-328460-thx9zh11	46	19	stopped	stop	VBN
cord-328460-thx9zh11	46	20	-	-	HYPH
cord-328460-thx9zh11	46	21	flow	flow	NN
cord-328460-thx9zh11	46	22	technology	technology	NN
cord-328460-thx9zh11	46	23	[	[	-LRB-
cord-328460-thx9zh11	46	24	18	18	CD
cord-328460-thx9zh11	46	25	]	]	-RRB-
cord-328460-thx9zh11	46	26	.	.	.
cord-328460-thx9zh11	47	1	Using	use	VBG
cord-328460-thx9zh11	47	2	a	a	DT
cord-328460-thx9zh11	47	3	high	high	JJ
cord-328460-thx9zh11	47	4	-	-	HYPH
cord-328460-thx9zh11	47	5	precision	precision	NN
cord-328460-thx9zh11	47	6	valve	valve	NN
cord-328460-thx9zh11	47	7	system	system	NN
cord-328460-thx9zh11	47	8	,	,	,
cord-328460-thx9zh11	47	9	the	the	DT
cord-328460-thx9zh11	47	10	stopped	stop	VBN
cord-328460-thx9zh11	47	11	-	-	HYPH
cord-328460-thx9zh11	47	12	flow	flow	NN
cord-328460-thx9zh11	47	13	format	format	NN
cord-328460-thx9zh11	47	14	enables	enable	VBZ
cord-328460-thx9zh11	47	15	the	the	DT
cord-328460-thx9zh11	47	16	monitoring	monitoring	NN
cord-328460-thx9zh11	47	17	of	of	IN
cord-328460-thx9zh11	47	18	dynamic	dynamic	JJ
cord-328460-thx9zh11	47	19	interactions	interaction	NNS
cord-328460-thx9zh11	47	20	of	of	IN
cord-328460-thx9zh11	47	21	biomolecules	biomolecule	NNS
cord-328460-thx9zh11	47	22	or	or	CC
cord-328460-thx9zh11	47	23	other	other	JJ
cord-328460-thx9zh11	47	24	macromolecules	macromolecule	NNS
cord-328460-thx9zh11	47	25	with	with	IN
cord-328460-thx9zh11	47	26	high	high	JJ
cord-328460-thx9zh11	47	27	temporal	temporal	JJ
cord-328460-thx9zh11	47	28	resolution	resolution	NN
cord-328460-thx9zh11	47	29	[	[	-LRB-
cord-328460-thx9zh11	47	30	19	19	CD
cord-328460-thx9zh11	47	31	]	]	-RRB-
cord-328460-thx9zh11	47	32	.	.	.
cord-328460-thx9zh11	48	1	Different	different	JJ
cord-328460-thx9zh11	48	2	microfluidic	microfluidic	NN
cord-328460-thx9zh11	48	3	-	-	HYPH
cord-328460-thx9zh11	48	4	coupled	couple	VBN
cord-328460-thx9zh11	48	5	PCR	pcr	NN
cord-328460-thx9zh11	48	6	amplification	amplification	NN
cord-328460-thx9zh11	48	7	approaches	approach	NNS
cord-328460-thx9zh11	48	8	have	have	VBP
cord-328460-thx9zh11	48	9	been	be	VBN
cord-328460-thx9zh11	48	10	developed	develop	VBN
cord-328460-thx9zh11	48	11	over	over	IN
cord-328460-thx9zh11	48	12	the	the	DT
cord-328460-thx9zh11	48	13	last	last	JJ
cord-328460-thx9zh11	48	14	few	few	JJ
cord-328460-thx9zh11	48	15	years	year	NNS
cord-328460-thx9zh11	48	16	[	[	-LRB-
cord-328460-thx9zh11	48	17	20	20	CD
cord-328460-thx9zh11	48	18	,	,	,
cord-328460-thx9zh11	48	19	21	21	CD
cord-328460-thx9zh11	48	20	]	]	-RRB-
cord-328460-thx9zh11	48	21	.	.	.
cord-328460-thx9zh11	49	1	These	these	DT
cord-328460-thx9zh11	49	2	also	also	RB
cord-328460-thx9zh11	49	3	include	include	VBP
cord-328460-thx9zh11	49	4	reverse	reverse	JJ
cord-328460-thx9zh11	49	5	transcription	transcription	NN
cord-328460-thx9zh11	49	6	polymerase	polymerase	NN
cord-328460-thx9zh11	49	7	chain	chain	NN
cord-328460-thx9zh11	49	8	reaction	reaction	NN
cord-328460-thx9zh11	49	9	(	(	-LRB-
cord-328460-thx9zh11	49	10	RT	RT	NNP
cord-328460-thx9zh11	49	11	-	-	HYPH
cord-328460-thx9zh11	49	12	PCR	PCR	NNP
cord-328460-thx9zh11	49	13	)	)	-RRB-
cord-328460-thx9zh11	49	14	for	for	IN
cord-328460-thx9zh11	49	15	RNA	rna	NN
cord-328460-thx9zh11	49	16	amplification	amplification	NN
cord-328460-thx9zh11	49	17	[	[	-LRB-
cord-328460-thx9zh11	49	18	22	22	CD
cord-328460-thx9zh11	49	19	,	,	,
cord-328460-thx9zh11	49	20	23	23	CD
cord-328460-thx9zh11	49	21	]	]	-RRB-
cord-328460-thx9zh11	49	22	.	.	.
cord-328460-thx9zh11	50	1	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	50	2	PCR	PCR	NNP
cord-328460-thx9zh11	50	3	enables	enable	VBZ
cord-328460-thx9zh11	50	4	sample	sample	NN
cord-328460-thx9zh11	50	5	manipulation	manipulation	NN
cord-328460-thx9zh11	50	6	and	and	CC
cord-328460-thx9zh11	50	7	detection	detection	NN
cord-328460-thx9zh11	50	8	to	to	TO
cord-328460-thx9zh11	50	9	be	be	VB
cord-328460-thx9zh11	50	10	integrated	integrate	VBN
cord-328460-thx9zh11	50	11	into	into	IN
cord-328460-thx9zh11	50	12	a	a	DT
cord-328460-thx9zh11	50	13	single	single	JJ
cord-328460-thx9zh11	50	14	device	device	NN
cord-328460-thx9zh11	50	15	and	and	CC
cord-328460-thx9zh11	50	16	is	be	VBZ
cord-328460-thx9zh11	50	17	associated	associate	VBN
cord-328460-thx9zh11	50	18	with	with	IN
cord-328460-thx9zh11	50	19	a	a	DT
cord-328460-thx9zh11	50	20	rapid	rapid	JJ
cord-328460-thx9zh11	50	21	heat	heat	NN
cord-328460-thx9zh11	50	22	transfer	transfer	NN
cord-328460-thx9zh11	50	23	,	,	,
cord-328460-thx9zh11	50	24	due	due	IN
cord-328460-thx9zh11	50	25	to	to	IN
cord-328460-thx9zh11	50	26	the	the	DT
cord-328460-thx9zh11	50	27	large	large	JJ
cord-328460-thx9zh11	50	28	surface	surface	NN
cord-328460-thx9zh11	50	29	-	-	HYPH
cord-328460-thx9zh11	50	30	to	to	IN
cord-328460-thx9zh11	50	31	-	-	HYPH
cord-328460-thx9zh11	50	32	volume	volume	NN
cord-328460-thx9zh11	50	33	ratio	ratio	NN
cord-328460-thx9zh11	50	34	obtained	obtain	VBN
cord-328460-thx9zh11	50	35	in	in	IN
cord-328460-thx9zh11	50	36	the	the	DT
cord-328460-thx9zh11	50	37	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	50	38	device	device	NN
cord-328460-thx9zh11	50	39	.	.	.
cord-328460-thx9zh11	51	1	The	the	DT
cord-328460-thx9zh11	51	2	latter	latter	JJ
cord-328460-thx9zh11	51	3	property	property	NN
cord-328460-thx9zh11	51	4	helps	help	VBZ
cord-328460-thx9zh11	51	5	in	in	IN
cord-328460-thx9zh11	51	6	significantly	significantly	RB
cord-328460-thx9zh11	51	7	reducing	reduce	VBG
cord-328460-thx9zh11	51	8	the	the	DT
cord-328460-thx9zh11	51	9	thermal	thermal	JJ
cord-328460-thx9zh11	51	10	-	-	HYPH
cord-328460-thx9zh11	51	11	cycling	cycling	NN
cord-328460-thx9zh11	51	12	time	time	NN
cord-328460-thx9zh11	51	13	while	while	IN
cord-328460-thx9zh11	51	14	increasing	increase	VBG
cord-328460-thx9zh11	51	15	the	the	DT
cord-328460-thx9zh11	51	16	amplification	amplification	NN
cord-328460-thx9zh11	51	17	reaction	reaction	NN
cord-328460-thx9zh11	51	18	yield	yield	NN
cord-328460-thx9zh11	51	19	as	as	IN
cord-328460-thx9zh11	51	20	a	a	DT
cord-328460-thx9zh11	51	21	consequence	consequence	NN
cord-328460-thx9zh11	51	22	of	of	IN
cord-328460-thx9zh11	51	23	the	the	DT
cord-328460-thx9zh11	51	24	more	more	RBR
cord-328460-thx9zh11	51	25	uniform	uniform	JJ
cord-328460-thx9zh11	51	26	heat	heat	NN
cord-328460-thx9zh11	51	27	transfer	transfer	NN
cord-328460-thx9zh11	51	28	to	to	IN
cord-328460-thx9zh11	51	29	the	the	DT
cord-328460-thx9zh11	51	30	PCR	PCR	NNP
cord-328460-thx9zh11	51	31	solution	solution	NN
cord-328460-thx9zh11	51	32	during	during	IN
cord-328460-thx9zh11	51	33	the	the	DT
cord-328460-thx9zh11	51	34	amplification	amplification	NN
cord-328460-thx9zh11	51	35	process	process	NN
cord-328460-thx9zh11	51	36	.	.	.
cord-328460-thx9zh11	52	1	On	on	IN
cord-328460-thx9zh11	52	2	the	the	DT
cord-328460-thx9zh11	52	3	other	other	JJ
cord-328460-thx9zh11	52	4	hand	hand	NN
cord-328460-thx9zh11	52	5	,	,	,
cord-328460-thx9zh11	52	6	the	the	DT
cord-328460-thx9zh11	52	7	non	non	JJ
cord-328460-thx9zh11	52	8	-	-	JJ
cord-328460-thx9zh11	52	9	specific	specific	JJ
cord-328460-thx9zh11	52	10	adsorption	adsorption	NN
cord-328460-thx9zh11	52	11	of	of	IN
cord-328460-thx9zh11	52	12	PCR	PCR	NNP
cord-328460-thx9zh11	52	13	mixture	mixture	NN
cord-328460-thx9zh11	52	14	components	component	NNS
cord-328460-thx9zh11	52	15	on	on	IN
cord-328460-thx9zh11	52	16	the	the	DT
cord-328460-thx9zh11	52	17	surface	surface	NN
cord-328460-thx9zh11	52	18	of	of	IN
cord-328460-thx9zh11	52	19	the	the	DT
cord-328460-thx9zh11	52	20	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	52	21	channels	channel	NNS
cord-328460-thx9zh11	52	22	becomes	become	VBZ
cord-328460-thx9zh11	52	23	an	an	DT
cord-328460-thx9zh11	52	24	important	important	JJ
cord-328460-thx9zh11	52	25	limitation	limitation	NN
cord-328460-thx9zh11	52	26	[	[	-LRB-
cord-328460-thx9zh11	52	27	24	24	CD
cord-328460-thx9zh11	52	28	]	]	-RRB-
cord-328460-thx9zh11	52	29	.	.	.
cord-328460-thx9zh11	53	1	In	in	IN
cord-328460-thx9zh11	53	2	fact	fact	NN
cord-328460-thx9zh11	53	3	,	,	,
cord-328460-thx9zh11	53	4	PCR	PCR	NNP
cord-328460-thx9zh11	53	5	sample	sample	NN
cord-328460-thx9zh11	53	6	interaction	interaction	NN
cord-328460-thx9zh11	53	7	with	with	IN
cord-328460-thx9zh11	53	8	the	the	DT
cord-328460-thx9zh11	53	9	surface	surface	NN
cord-328460-thx9zh11	53	10	can	can	MD
cord-328460-thx9zh11	53	11	produce	produce	VB
cord-328460-thx9zh11	53	12	a	a	DT
cord-328460-thx9zh11	53	13	partial	partial	JJ
cord-328460-thx9zh11	53	14	inhibition	inhibition	NN
cord-328460-thx9zh11	53	15	of	of	IN
cord-328460-thx9zh11	53	16	the	the	DT
cord-328460-thx9zh11	53	17	PCR	PCR	NNP
cord-328460-thx9zh11	53	18	enzyme	enzyme	NN
cord-328460-thx9zh11	53	19	and	and	CC
cord-328460-thx9zh11	53	20	can	can	MD
cord-328460-thx9zh11	53	21	carryover	carryover	VB
cord-328460-thx9zh11	53	22	contamination	contamination	NN
cord-328460-thx9zh11	53	23	problems	problem	NNS
cord-328460-thx9zh11	53	24	[	[	-LRB-
cord-328460-thx9zh11	53	25	20	20	CD
cord-328460-thx9zh11	53	26	]	]	-RRB-
cord-328460-thx9zh11	53	27	.	.	.
cord-328460-thx9zh11	54	1	Silicon	silicon	NN
cord-328460-thx9zh11	54	2	/	/	SYM
cord-328460-thx9zh11	54	3	glass	glass	NN
cord-328460-thx9zh11	54	4	-	-	HYPH
cord-328460-thx9zh11	54	5	based	base	VBN
cord-328460-thx9zh11	54	6	PCR	PCR	NNP
cord-328460-thx9zh11	54	7	microchips	microchip	NNS
cord-328460-thx9zh11	54	8	have	have	VBP
cord-328460-thx9zh11	54	9	shown	show	VBN
cord-328460-thx9zh11	54	10	partial	partial	JJ
cord-328460-thx9zh11	54	11	or	or	CC
cord-328460-thx9zh11	54	12	total	total	JJ
cord-328460-thx9zh11	54	13	inhibition	inhibition	NN
cord-328460-thx9zh11	54	14	of	of	IN
cord-328460-thx9zh11	54	15	the	the	DT
cord-328460-thx9zh11	54	16	PCR	PCR	NNP
cord-328460-thx9zh11	54	17	amplification	amplification	NN
cord-328460-thx9zh11	54	18	caused	cause	VBN
cord-328460-thx9zh11	54	19	by	by	IN
cord-328460-thx9zh11	54	20	the	the	DT
cord-328460-thx9zh11	54	21	interaction	interaction	NN
cord-328460-thx9zh11	54	22	between	between	IN
cord-328460-thx9zh11	54	23	the	the	DT
cord-328460-thx9zh11	54	24	chip	chip	NN
cord-328460-thx9zh11	54	25	surface	surface	NN
cord-328460-thx9zh11	54	26	and	and	CC
cord-328460-thx9zh11	54	27	PCR	PCR	NNP
cord-328460-thx9zh11	54	28	components	component	NNS
cord-328460-thx9zh11	54	29	[	[	-LRB-
cord-328460-thx9zh11	54	30	25	25	CD
cord-328460-thx9zh11	54	31	]	]	-RRB-
cord-328460-thx9zh11	54	32	.	.	.
cord-328460-thx9zh11	55	1	Surface	surface	NN
cord-328460-thx9zh11	55	2	adsorption	adsorption	NN
cord-328460-thx9zh11	55	3	has	have	VBZ
cord-328460-thx9zh11	55	4	been	be	VBN
cord-328460-thx9zh11	55	5	proposed	propose	VBN
cord-328460-thx9zh11	55	6	as	as	IN
cord-328460-thx9zh11	55	7	the	the	DT
cord-328460-thx9zh11	55	8	phenomenon	phenomenon	NN
cord-328460-thx9zh11	55	9	responsible	responsible	JJ
cord-328460-thx9zh11	55	10	for	for	IN
cord-328460-thx9zh11	55	11	the	the	DT
cord-328460-thx9zh11	55	12	inhibition	inhibition	NN
cord-328460-thx9zh11	55	13	.	.	.
cord-328460-thx9zh11	56	1	In	in	IN
cord-328460-thx9zh11	56	2	particular	particular	JJ
cord-328460-thx9zh11	56	3	,	,	,
cord-328460-thx9zh11	56	4	it	-PRON-	PRP
cord-328460-thx9zh11	56	5	has	have	VBZ
cord-328460-thx9zh11	56	6	been	be	VBN
cord-328460-thx9zh11	56	7	shown	show	VBN
cord-328460-thx9zh11	56	8	that	that	IN
cord-328460-thx9zh11	56	9	the	the	DT
cord-328460-thx9zh11	56	10	PCR	PCR	NNP
cord-328460-thx9zh11	56	11	inhibition	inhibition	NN
cord-328460-thx9zh11	56	12	effect	effect	NN
cord-328460-thx9zh11	56	13	is	be	VBZ
cord-328460-thx9zh11	56	14	due	due	JJ
cord-328460-thx9zh11	56	15	to	to	IN
cord-328460-thx9zh11	56	16	a	a	DT
cord-328460-thx9zh11	56	17	non	non	JJ
cord-328460-thx9zh11	56	18	-	-	JJ
cord-328460-thx9zh11	56	19	specific	specific	JJ
cord-328460-thx9zh11	56	20	sequestering	sequestering	NN
cord-328460-thx9zh11	56	21	of	of	IN
cord-328460-thx9zh11	56	22	Taq	taq	NN
cord-328460-thx9zh11	56	23	polymerase	polymerase	NN
cord-328460-thx9zh11	56	24	which	which	WDT
cord-328460-thx9zh11	56	25	prevents	prevent	VBZ
cord-328460-thx9zh11	56	26	the	the	DT
cord-328460-thx9zh11	56	27	enzyme	enzyme	NN
cord-328460-thx9zh11	56	28	activity	activity	NN
cord-328460-thx9zh11	56	29	and	and	CC
cord-328460-thx9zh11	56	30	thereby	thereby	RB
cord-328460-thx9zh11	56	31	the	the	DT
cord-328460-thx9zh11	56	32	DNA	dna	NN
cord-328460-thx9zh11	56	33	amplification	amplification	NN
cord-328460-thx9zh11	56	34	.	.	.
cord-328460-thx9zh11	57	1	At	at	IN
cord-328460-thx9zh11	57	2	the	the	DT
cord-328460-thx9zh11	57	3	same	same	JJ
cord-328460-thx9zh11	57	4	time	time	NN
cord-328460-thx9zh11	57	5	,	,	,
cord-328460-thx9zh11	57	6	DNA	DNA	NNP
cord-328460-thx9zh11	57	7	is	be	VBZ
cord-328460-thx9zh11	57	8	not	not	RB
cord-328460-thx9zh11	57	9	significantly	significantly	RB
cord-328460-thx9zh11	57	10	adsorbed	adsorb	VBN
cord-328460-thx9zh11	57	11	on	on	IN
cord-328460-thx9zh11	57	12	the	the	DT
cord-328460-thx9zh11	57	13	surface	surface	NN
cord-328460-thx9zh11	57	14	[	[	-LRB-
cord-328460-thx9zh11	57	15	26	26	CD
cord-328460-thx9zh11	57	16	,	,	,
cord-328460-thx9zh11	57	17	27	27	CD
cord-328460-thx9zh11	57	18	]	]	-RRB-
cord-328460-thx9zh11	57	19	.	.	.
cord-328460-thx9zh11	58	1	Improvement	improvement	NN
cord-328460-thx9zh11	58	2	of	of	IN
cord-328460-thx9zh11	58	3	the	the	DT
cord-328460-thx9zh11	58	4	PCR	PCR	NNP
cord-328460-thx9zh11	58	5	efficiencies	efficiency	NNS
cord-328460-thx9zh11	58	6	can	can	MD
cord-328460-thx9zh11	58	7	be	be	VB
cord-328460-thx9zh11	58	8	accomplished	accomplish	VBN
cord-328460-thx9zh11	58	9	through	through	IN
cord-328460-thx9zh11	58	10	passivation	passivation	NN
cord-328460-thx9zh11	58	11	procedures	procedure	NNS
cord-328460-thx9zh11	58	12	[	[	-LRB-
cord-328460-thx9zh11	58	13	28	28	CD
cord-328460-thx9zh11	58	14	,	,	,
cord-328460-thx9zh11	58	15	29	29	CD
cord-328460-thx9zh11	58	16	]	]	-RRB-
cord-328460-thx9zh11	58	17	,	,	,
cord-328460-thx9zh11	58	18	either	either	CC
cord-328460-thx9zh11	58	19	by	by	IN
cord-328460-thx9zh11	58	20	static	static	JJ
cord-328460-thx9zh11	58	21	coating	coating	NN
cord-328460-thx9zh11	58	22	,	,	,
cord-328460-thx9zh11	58	23	where	where	WRB
cord-328460-thx9zh11	58	24	the	the	DT
cord-328460-thx9zh11	58	25	inner	inner	JJ
cord-328460-thx9zh11	58	26	surface	surface	NN
cord-328460-thx9zh11	58	27	of	of	IN
cord-328460-thx9zh11	58	28	microchips	microchip	NNS
cord-328460-thx9zh11	58	29	is	be	VBZ
cord-328460-thx9zh11	58	30	coated	coat	VBN
cord-328460-thx9zh11	58	31	with	with	IN
cord-328460-thx9zh11	58	32	a	a	DT
cord-328460-thx9zh11	58	33	"	"	``
cord-328460-thx9zh11	58	34	PCR	PCR	NNP
cord-328460-thx9zh11	58	35	friendly	friendly	JJ
cord-328460-thx9zh11	58	36	"	"	''
cord-328460-thx9zh11	58	37	material	material	NN
cord-328460-thx9zh11	58	38	(	(	-LRB-
cord-328460-thx9zh11	58	39	e.g.	e.g.	RB
cord-328460-thx9zh11	58	40	,	,	,
cord-328460-thx9zh11	58	41	silanization	silanization	NN
cord-328460-thx9zh11	58	42	[	[	-LRB-
cord-328460-thx9zh11	58	43	30	30	CD
cord-328460-thx9zh11	58	44	]	]	-RRB-
cord-328460-thx9zh11	58	45	or	or	CC
cord-328460-thx9zh11	58	46	deposition	deposition	NN
cord-328460-thx9zh11	58	47	of	of	IN
cord-328460-thx9zh11	58	48	oxide	oxide	NN
cord-328460-thx9zh11	58	49	layers	layer	NNS
cord-328460-thx9zh11	58	50	[	[	-LRB-
cord-328460-thx9zh11	58	51	26	26	CD
cord-328460-thx9zh11	58	52	,	,	,
cord-328460-thx9zh11	58	53	27	27	CD
cord-328460-thx9zh11	58	54	]	]	-RRB-
cord-328460-thx9zh11	58	55	)	)	-RRB-
cord-328460-thx9zh11	58	56	,	,	,
cord-328460-thx9zh11	58	57	or	or	CC
cord-328460-thx9zh11	58	58	dynamic	dynamic	JJ
cord-328460-thx9zh11	58	59	coating	coating	NN
cord-328460-thx9zh11	58	60	,	,	,
cord-328460-thx9zh11	58	61	in	in	IN
cord-328460-thx9zh11	58	62	which	which	WDT
cord-328460-thx9zh11	58	63	specific	specific	JJ
cord-328460-thx9zh11	58	64	components	component	NNS
cord-328460-thx9zh11	58	65	are	be	VBP
cord-328460-thx9zh11	58	66	added	add	VBN
cord-328460-thx9zh11	58	67	to	to	IN
cord-328460-thx9zh11	58	68	the	the	DT
cord-328460-thx9zh11	58	69	reaction	reaction	NN
cord-328460-thx9zh11	58	70	mixture	mixture	NN
cord-328460-thx9zh11	58	71	(	(	-LRB-
cord-328460-thx9zh11	58	72	e.g.	e.g.	RB
cord-328460-thx9zh11	58	73	,	,	,
cord-328460-thx9zh11	58	74	polyethylene	polyethylene	NN
cord-328460-thx9zh11	58	75	glycol	glycol	NN
cord-328460-thx9zh11	58	76	[	[	-LRB-
cord-328460-thx9zh11	58	77	31	31	CD
cord-328460-thx9zh11	58	78	]	]	-RRB-
cord-328460-thx9zh11	58	79	,	,	,
cord-328460-thx9zh11	58	80	polyvinylpyrrolidone	polyvinylpyrrolidone	NN
cord-328460-thx9zh11	58	81	,	,	,
cord-328460-thx9zh11	58	82	bovine	bovine	JJ
cord-328460-thx9zh11	58	83	serum	serum	NN
cord-328460-thx9zh11	58	84	albumin	albumin	NN
cord-328460-thx9zh11	58	85	[	[	-LRB-
cord-328460-thx9zh11	58	86	26	26	CD
cord-328460-thx9zh11	58	87	,	,	,
cord-328460-thx9zh11	58	88	27	27	CD
cord-328460-thx9zh11	58	89	]	]	-RRB-
cord-328460-thx9zh11	58	90	)	)	-RRB-
cord-328460-thx9zh11	58	91	.	.	.
cord-328460-thx9zh11	59	1	The	the	DT
cord-328460-thx9zh11	59	2	introduction	introduction	NN
cord-328460-thx9zh11	59	3	of	of	IN
cord-328460-thx9zh11	59	4	a	a	DT
cord-328460-thx9zh11	59	5	droplet	droplet	NN
cord-328460-thx9zh11	59	6	-	-	HYPH
cord-328460-thx9zh11	59	7	based	base	VBN
cord-328460-thx9zh11	59	8	technology	technology	NN
cord-328460-thx9zh11	59	9	provides	provide	VBZ
cord-328460-thx9zh11	59	10	a	a	DT
cord-328460-thx9zh11	59	11	convenient	convenient	JJ
cord-328460-thx9zh11	59	12	way	way	NN
cord-328460-thx9zh11	59	13	to	to	TO
cord-328460-thx9zh11	59	14	prevent	prevent	VB
cord-328460-thx9zh11	59	15	most	most	JJS
cord-328460-thx9zh11	59	16	of	of	IN
cord-328460-thx9zh11	59	17	the	the	DT
cord-328460-thx9zh11	59	18	above	above	RB
cord-328460-thx9zh11	59	19	-	-	HYPH
cord-328460-thx9zh11	59	20	mentioned	mention	VBN
cord-328460-thx9zh11	59	21	issues	issue	NNS
cord-328460-thx9zh11	59	22	since	since	IN
cord-328460-thx9zh11	59	23	amplification	amplification	NN
cord-328460-thx9zh11	59	24	reactions	reaction	NNS
cord-328460-thx9zh11	59	25	occur	occur	VBP
cord-328460-thx9zh11	59	26	within	within	IN
cord-328460-thx9zh11	59	27	droplets	droplet	NNS
cord-328460-thx9zh11	59	28	.	.	.
cord-328460-thx9zh11	60	1	Any	any	DT
cord-328460-thx9zh11	60	2	interaction	interaction	NN
cord-328460-thx9zh11	60	3	between	between	IN
cord-328460-thx9zh11	60	4	the	the	DT
cord-328460-thx9zh11	60	5	PCR	PCR	NNP
cord-328460-thx9zh11	60	6	sample	sample	NN
cord-328460-thx9zh11	60	7	and	and	CC
cord-328460-thx9zh11	60	8	the	the	DT
cord-328460-thx9zh11	60	9	surface	surface	NN
cord-328460-thx9zh11	60	10	of	of	IN
cord-328460-thx9zh11	60	11	the	the	DT
cord-328460-thx9zh11	60	12	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	60	13	device	device	NN
cord-328460-thx9zh11	60	14	is	be	VBZ
cord-328460-thx9zh11	60	15	eliminated	eliminate	VBN
cord-328460-thx9zh11	60	16	.	.	.
cord-328460-thx9zh11	61	1	Droplet	droplet	NN
cord-328460-thx9zh11	61	2	-	-	HYPH
cord-328460-thx9zh11	61	3	based	base	VBN
cord-328460-thx9zh11	61	4	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	61	5	,	,	,
cord-328460-thx9zh11	61	6	unlike	unlike	IN
cord-328460-thx9zh11	61	7	continuous	continuous	JJ
cord-328460-thx9zh11	61	8	flow	flow	NN
cord-328460-thx9zh11	61	9	systems	system	NNS
cord-328460-thx9zh11	61	10	,	,	,
cord-328460-thx9zh11	61	11	creates	create	VBZ
cord-328460-thx9zh11	61	12	discrete	discrete	JJ
cord-328460-thx9zh11	61	13	fraction	fraction	NN
cord-328460-thx9zh11	61	14	of	of	IN
cord-328460-thx9zh11	61	15	water	water	NN
cord-328460-thx9zh11	61	16	solutions	solution	NNS
cord-328460-thx9zh11	61	17	by	by	IN
cord-328460-thx9zh11	61	18	using	use	VBG
cord-328460-thx9zh11	61	19	immiscible	immiscible	JJ
cord-328460-thx9zh11	61	20	fluids	fluid	NNS
cord-328460-thx9zh11	61	21	that	that	WDT
cord-328460-thx9zh11	61	22	are	be	VBP
cord-328460-thx9zh11	61	23	driven	drive	VBN
cord-328460-thx9zh11	61	24	into	into	IN
cord-328460-thx9zh11	61	25	separate	separate	JJ
cord-328460-thx9zh11	61	26	microchannels	microchannel	NNS
cord-328460-thx9zh11	61	27	via	via	IN
cord-328460-thx9zh11	61	28	an	an	DT
cord-328460-thx9zh11	61	29	independently	independently	RB
cord-328460-thx9zh11	61	30	controlled	control	VBN
cord-328460-thx9zh11	61	31	flow	flow	NN
cord-328460-thx9zh11	61	32	[	[	-LRB-
cord-328460-thx9zh11	61	33	32	32	CD
cord-328460-thx9zh11	61	34	]	]	-RRB-
cord-328460-thx9zh11	61	35	.	.	.
cord-328460-thx9zh11	62	1	Most	Most	JJS
cord-328460-thx9zh11	62	2	of	of	IN
cord-328460-thx9zh11	62	3	the	the	DT
cord-328460-thx9zh11	62	4	adopted	adopt	VBN
cord-328460-thx9zh11	62	5	methods	method	NNS
cord-328460-thx9zh11	62	6	produce	produce	VBP
cord-328460-thx9zh11	62	7	droplets	droplet	NNS
cord-328460-thx9zh11	62	8	with	with	IN
cord-328460-thx9zh11	62	9	diameters	diameter	NNS
cord-328460-thx9zh11	62	10	ranging	range	VBG
cord-328460-thx9zh11	62	11	from	from	IN
cord-328460-thx9zh11	62	12	a	a	DT
cord-328460-thx9zh11	62	13	few	few	JJ
cord-328460-thx9zh11	62	14	micrometers	micrometer	NNS
cord-328460-thx9zh11	62	15	to	to	IN
cord-328460-thx9zh11	62	16	hundreds	hundred	NNS
cord-328460-thx9zh11	62	17	of	of	IN
cord-328460-thx9zh11	62	18	micrometers	micrometer	NNS
cord-328460-thx9zh11	62	19	.	.	.
cord-328460-thx9zh11	63	1	Polydispersity	Polydispersity	NNP
cord-328460-thx9zh11	63	2	,	,	,
cord-328460-thx9zh11	63	3	defined	define	VBN
cord-328460-thx9zh11	63	4	as	as	IN
cord-328460-thx9zh11	63	5	the	the	DT
cord-328460-thx9zh11	63	6	standard	standard	JJ
cord-328460-thx9zh11	63	7	deviation	deviation	NN
cord-328460-thx9zh11	63	8	of	of	IN
cord-328460-thx9zh11	63	9	the	the	DT
cord-328460-thx9zh11	63	10	size	size	NN
cord-328460-thx9zh11	63	11	distribution	distribution	NN
cord-328460-thx9zh11	63	12	divided	divide	VBN
cord-328460-thx9zh11	63	13	by	by	IN
cord-328460-thx9zh11	63	14	the	the	DT
cord-328460-thx9zh11	63	15	mean	mean	JJ
cord-328460-thx9zh11	63	16	droplet	droplet	NN
cord-328460-thx9zh11	63	17	size	size	NN
cord-328460-thx9zh11	63	18	,	,	,
cord-328460-thx9zh11	63	19	can	can	MD
cord-328460-thx9zh11	63	20	be	be	VB
cord-328460-thx9zh11	63	21	1	1	CD
cord-328460-thx9zh11	63	22	-	-	SYM
cord-328460-thx9zh11	63	23	3	3	CD
cord-328460-thx9zh11	63	24	%	%	NN
cord-328460-thx9zh11	63	25	while	while	IN
cord-328460-thx9zh11	63	26	droplet	droplet	NN
cord-328460-thx9zh11	63	27	volumes	volume	NNS
cord-328460-thx9zh11	63	28	range	range	VBP
cord-328460-thx9zh11	63	29	from	from	IN
cord-328460-thx9zh11	63	30	femtoliters	femtoliter	NNS
cord-328460-thx9zh11	63	31	to	to	IN
cord-328460-thx9zh11	63	32	nanoliters	nanoliter	NNS
cord-328460-thx9zh11	63	33	[	[	-LRB-
cord-328460-thx9zh11	63	34	33	33	CD
cord-328460-thx9zh11	63	35	]	]	-RRB-
cord-328460-thx9zh11	63	36	.	.	.
cord-328460-thx9zh11	64	1	Factors	factor	NNS
cord-328460-thx9zh11	64	2	such	such	JJ
cord-328460-thx9zh11	64	3	as	as	IN
cord-328460-thx9zh11	64	4	the	the	DT
cord-328460-thx9zh11	64	5	use	use	NN
cord-328460-thx9zh11	64	6	of	of	IN
cord-328460-thx9zh11	64	7	surfactants	surfactant	NNS
cord-328460-thx9zh11	64	8	,	,	,
cord-328460-thx9zh11	64	9	the	the	DT
cord-328460-thx9zh11	64	10	viscosity	viscosity	NN
cord-328460-thx9zh11	64	11	of	of	IN
cord-328460-thx9zh11	64	12	the	the	DT
cord-328460-thx9zh11	64	13	immiscible	immiscible	JJ
cord-328460-thx9zh11	64	14	phase	phase	NN
cord-328460-thx9zh11	64	15	,	,	,
cord-328460-thx9zh11	64	16	and	and	CC
cord-328460-thx9zh11	64	17	the	the	DT
cord-328460-thx9zh11	64	18	wettability	wettability	NN
cord-328460-thx9zh11	64	19	of	of	IN
cord-328460-thx9zh11	64	20	the	the	DT
cord-328460-thx9zh11	64	21	channel	channel	NN
cord-328460-thx9zh11	64	22	walls	wall	NNS
cord-328460-thx9zh11	64	23	can	can	MD
cord-328460-thx9zh11	64	24	be	be	VB
cord-328460-thx9zh11	64	25	used	use	VBN
cord-328460-thx9zh11	64	26	to	to	TO
cord-328460-thx9zh11	64	27	manage	manage	VB
cord-328460-thx9zh11	64	28	the	the	DT
cord-328460-thx9zh11	64	29	size	size	NN
cord-328460-thx9zh11	64	30	of	of	IN
cord-328460-thx9zh11	64	31	the	the	DT
cord-328460-thx9zh11	64	32	formed	form	VBN
cord-328460-thx9zh11	64	33	droplets	droplet	NNS
cord-328460-thx9zh11	64	34	.	.	.
cord-328460-thx9zh11	65	1	The	the	DT
cord-328460-thx9zh11	65	2	hydrophilicity	hydrophilicity	NN
cord-328460-thx9zh11	65	3	or	or	CC
cord-328460-thx9zh11	65	4	hydrophobicity	hydrophobicity	NN
cord-328460-thx9zh11	65	5	of	of	IN
cord-328460-thx9zh11	65	6	the	the	DT
cord-328460-thx9zh11	65	7	microchannel	microchannel	NN
cord-328460-thx9zh11	65	8	surface	surface	NN
cord-328460-thx9zh11	65	9	determines	determine	VBZ
cord-328460-thx9zh11	65	10	which	which	WDT
cord-328460-thx9zh11	65	11	liquid	liquid	NN
cord-328460-thx9zh11	65	12	phase	phase	NN
cord-328460-thx9zh11	65	13	is	be	VBZ
cord-328460-thx9zh11	65	14	dispersed	disperse	VBN
cord-328460-thx9zh11	65	15	.	.	.
cord-328460-thx9zh11	66	1	The	the	DT
cord-328460-thx9zh11	66	2	ability	ability	NN
cord-328460-thx9zh11	66	3	to	to	TO
cord-328460-thx9zh11	66	4	rapidly	rapidly	RB
cord-328460-thx9zh11	66	5	create	create	VB
cord-328460-thx9zh11	66	6	highly	highly	RB
cord-328460-thx9zh11	66	7	uniform	uniform	JJ
cord-328460-thx9zh11	66	8	aqueous	aqueous	JJ
cord-328460-thx9zh11	66	9	droplets	droplet	NNS
cord-328460-thx9zh11	66	10	with	with	IN
cord-328460-thx9zh11	66	11	controlled	control	VBN
cord-328460-thx9zh11	66	12	contents	content	NNS
cord-328460-thx9zh11	66	13	enables	enable	VBZ
cord-328460-thx9zh11	66	14	the	the	DT
cord-328460-thx9zh11	66	15	rapid	rapid	JJ
cord-328460-thx9zh11	66	16	analysis	analysis	NN
cord-328460-thx9zh11	66	17	of	of	IN
cord-328460-thx9zh11	66	18	very	very	RB
cord-328460-thx9zh11	66	19	small	small	JJ
cord-328460-thx9zh11	66	20	quantities	quantity	NNS
cord-328460-thx9zh11	66	21	of	of	IN
cord-328460-thx9zh11	66	22	reagents	reagent	NNS
cord-328460-thx9zh11	66	23	in	in	IN
cord-328460-thx9zh11	66	24	a	a	DT
cord-328460-thx9zh11	66	25	portable	portable	JJ
cord-328460-thx9zh11	66	26	,	,	,
cord-328460-thx9zh11	66	27	automated	automated	JJ
cord-328460-thx9zh11	66	28	and	and	CC
cord-328460-thx9zh11	66	29	inexpensive	inexpensive	JJ
cord-328460-thx9zh11	66	30	format	format	NN
cord-328460-thx9zh11	66	31	.	.	.
cord-328460-thx9zh11	67	1	Reagents	reagent	NNS
cord-328460-thx9zh11	67	2	are	be	VBP
cord-328460-thx9zh11	67	3	confined	confine	VBN
cord-328460-thx9zh11	67	4	in	in	IN
cord-328460-thx9zh11	67	5	droplets	droplet	NNS
cord-328460-thx9zh11	67	6	and	and	CC
cord-328460-thx9zh11	67	7	each	each	DT
cord-328460-thx9zh11	67	8	droplet	droplet	NN
cord-328460-thx9zh11	67	9	is	be	VBZ
cord-328460-thx9zh11	67	10	isolated	isolate	VBN
cord-328460-thx9zh11	67	11	from	from	IN
cord-328460-thx9zh11	67	12	channel	channel	NN
cord-328460-thx9zh11	67	13	walls	wall	NNS
cord-328460-thx9zh11	67	14	by	by	IN
cord-328460-thx9zh11	67	15	the	the	DT
cord-328460-thx9zh11	67	16	immiscible	immiscible	JJ
cord-328460-thx9zh11	67	17	liquid	liquid	NN
cord-328460-thx9zh11	67	18	.	.	.
cord-328460-thx9zh11	68	1	A	a	DT
cord-328460-thx9zh11	68	2	similar	similar	JJ
cord-328460-thx9zh11	68	3	configuration	configuration	NN
cord-328460-thx9zh11	68	4	greatly	greatly	RB
cord-328460-thx9zh11	68	5	reduce	reduce	VBP
cord-328460-thx9zh11	68	6	the	the	DT
cord-328460-thx9zh11	68	7	reagent	reagent	JJ
cord-328460-thx9zh11	68	8	dispersion	dispersion	NN
cord-328460-thx9zh11	68	9	and	and	CC
cord-328460-thx9zh11	68	10	sample	sample	NN
cord-328460-thx9zh11	68	11	contaminations	contamination	NNS
cord-328460-thx9zh11	68	12	thus	thus	RB
cord-328460-thx9zh11	68	13	offering	offer	VBG
cord-328460-thx9zh11	68	14	fundamental	fundamental	JJ
cord-328460-thx9zh11	68	15	advantages	advantage	NNS
cord-328460-thx9zh11	68	16	for	for	IN
cord-328460-thx9zh11	68	17	successful	successful	JJ
cord-328460-thx9zh11	68	18	single	single	JJ
cord-328460-thx9zh11	68	19	-	-	HYPH
cord-328460-thx9zh11	68	20	molecule	molecule	NN
cord-328460-thx9zh11	68	21	DNA	dna	NN
cord-328460-thx9zh11	68	22	amplification	amplification	NN
cord-328460-thx9zh11	68	23	.	.	.
cord-328460-thx9zh11	69	1	Moreover	moreover	RB
cord-328460-thx9zh11	69	2	,	,	,
cord-328460-thx9zh11	69	3	each	each	DT
cord-328460-thx9zh11	69	4	droplet	droplet	NN
cord-328460-thx9zh11	69	5	can	can	MD
cord-328460-thx9zh11	69	6	be	be	VB
cord-328460-thx9zh11	69	7	considered	consider	VBN
cord-328460-thx9zh11	69	8	as	as	IN
cord-328460-thx9zh11	69	9	an	an	DT
cord-328460-thx9zh11	69	10	isolated	isolate	VBN
cord-328460-thx9zh11	69	11	microreactor	microreactor	NN
cord-328460-thx9zh11	69	12	and	and	CC
cord-328460-thx9zh11	69	13	each	each	DT
cord-328460-thx9zh11	69	14	microreactor	microreactor	NN
cord-328460-thx9zh11	69	15	can	can	MD
cord-328460-thx9zh11	69	16	be	be	VB
cord-328460-thx9zh11	69	17	potentially	potentially	RB
cord-328460-thx9zh11	69	18	individually	individually	RB
cord-328460-thx9zh11	69	19	controlled	control	VBN
cord-328460-thx9zh11	69	20	and	and	CC
cord-328460-thx9zh11	69	21	analyzed	analyze	VBD
cord-328460-thx9zh11	69	22	[	[	-LRB-
cord-328460-thx9zh11	69	23	34	34	CD
cord-328460-thx9zh11	69	24	,	,	,
cord-328460-thx9zh11	69	25	35	35	CD
cord-328460-thx9zh11	69	26	]	]	-RRB-
cord-328460-thx9zh11	69	27	by	by	IN
cord-328460-thx9zh11	69	28	allowing	allow	VBG
cord-328460-thx9zh11	69	29	an	an	DT
cord-328460-thx9zh11	69	30	easier	easy	JJR
cord-328460-thx9zh11	69	31	parallel	parallel	JJ
cord-328460-thx9zh11	69	32	processing	processing	NN
cord-328460-thx9zh11	69	33	[	[	-LRB-
cord-328460-thx9zh11	69	34	36	36	CD
cord-328460-thx9zh11	69	35	]	]	-RRB-
cord-328460-thx9zh11	69	36	,	,	,
cord-328460-thx9zh11	69	37	higher	high	JJR
cord-328460-thx9zh11	69	38	throughput	throughput	NN
cord-328460-thx9zh11	69	39	[	[	-LRB-
cord-328460-thx9zh11	69	40	37	37	CD
cord-328460-thx9zh11	69	41	]	]	-RRB-
cord-328460-thx9zh11	69	42	and	and	CC
cord-328460-thx9zh11	69	43	reduced	reduce	VBN
cord-328460-thx9zh11	69	44	sample	sample	NN
cord-328460-thx9zh11	69	45	consumption	consumption	NN
cord-328460-thx9zh11	69	46	with	with	IN
cord-328460-thx9zh11	69	47	respect	respect	NN
cord-328460-thx9zh11	69	48	to	to	IN
cord-328460-thx9zh11	69	49	the	the	DT
cord-328460-thx9zh11	69	50	continuous	continuous	JJ
cord-328460-thx9zh11	69	51	flow	flow	NN
cord-328460-thx9zh11	69	52	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	69	53	control	control	NN
cord-328460-thx9zh11	69	54	.	.	.
cord-328460-thx9zh11	70	1	It	-PRON-	PRP
cord-328460-thx9zh11	70	2	is	be	VBZ
cord-328460-thx9zh11	70	3	also	also	RB
cord-328460-thx9zh11	70	4	possible	possible	JJ
cord-328460-thx9zh11	70	5	to	to	TO
cord-328460-thx9zh11	70	6	split	split	VB
cord-328460-thx9zh11	70	7	the	the	DT
cord-328460-thx9zh11	70	8	single	single	JJ
cord-328460-thx9zh11	70	9	droplet	droplet	NN
cord-328460-thx9zh11	70	10	into	into	IN
cord-328460-thx9zh11	70	11	two	two	CD
cord-328460-thx9zh11	70	12	or	or	CC
cord-328460-thx9zh11	70	13	more	more	JJR
cord-328460-thx9zh11	70	14	droplets	droplet	NNS
cord-328460-thx9zh11	70	15	or	or	CC
cord-328460-thx9zh11	70	16	keeping	keep	VBG
cord-328460-thx9zh11	70	17	reagents	reagent	NNS
cord-328460-thx9zh11	70	18	separate	separate	JJ
cord-328460-thx9zh11	70	19	until	until	IN
cord-328460-thx9zh11	70	20	the	the	DT
cord-328460-thx9zh11	70	21	proper	proper	JJ
cord-328460-thx9zh11	70	22	conditions	condition	NNS
cord-328460-thx9zh11	70	23	are	be	VBP
cord-328460-thx9zh11	70	24	available	available	JJ
cord-328460-thx9zh11	70	25	and	and	CC
cord-328460-thx9zh11	70	26	then	then	RB
cord-328460-thx9zh11	70	27	performing	perform	VBG
cord-328460-thx9zh11	70	28	a	a	DT
cord-328460-thx9zh11	70	29	controlled	control	VBN
cord-328460-thx9zh11	70	30	coalescence	coalescence	NN
cord-328460-thx9zh11	70	31	of	of	IN
cord-328460-thx9zh11	70	32	droplets	droplet	NNS
cord-328460-thx9zh11	70	33	.	.	.
cord-328460-thx9zh11	71	1	Both	both	DT
cord-328460-thx9zh11	71	2	droplet	droplet	NN
cord-328460-thx9zh11	71	3	fission	fission	NN
cord-328460-thx9zh11	71	4	and	and	CC
cord-328460-thx9zh11	71	5	fusion	fusion	NN
cord-328460-thx9zh11	71	6	enhance	enhance	VB
cord-328460-thx9zh11	71	7	the	the	DT
cord-328460-thx9zh11	71	8	experimental	experimental	JJ
cord-328460-thx9zh11	71	9	capabilities	capability	NNS
cord-328460-thx9zh11	71	10	of	of	IN
cord-328460-thx9zh11	71	11	the	the	DT
cord-328460-thx9zh11	71	12	droplet	droplet	NN
cord-328460-thx9zh11	71	13	-	-	HYPH
cord-328460-thx9zh11	71	14	based	base	VBN
cord-328460-thx9zh11	71	15	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	71	16	approach	approach	NN
cord-328460-thx9zh11	71	17	.	.	.
cord-328460-thx9zh11	72	1	Droplets	droplet	NNS
cord-328460-thx9zh11	72	2	help	help	VBP
cord-328460-thx9zh11	72	3	in	in	IN
cord-328460-thx9zh11	72	4	achieving	achieve	VBG
cord-328460-thx9zh11	72	5	a	a	DT
cord-328460-thx9zh11	72	6	rapid	rapid	JJ
cord-328460-thx9zh11	72	7	mixing	mixing	NN
cord-328460-thx9zh11	72	8	of	of	IN
cord-328460-thx9zh11	72	9	reagents	reagent	NNS
cord-328460-thx9zh11	72	10	:	:	:
cord-328460-thx9zh11	72	11	the	the	DT
cord-328460-thx9zh11	72	12	rate	rate	NN
cord-328460-thx9zh11	72	13	of	of	IN
cord-328460-thx9zh11	72	14	DNA	dna	NN
cord-328460-thx9zh11	72	15	hybridization	hybridization	NN
cord-328460-thx9zh11	72	16	in	in	IN
cord-328460-thx9zh11	72	17	a	a	DT
cord-328460-thx9zh11	72	18	homogeneous	homogeneous	JJ
cord-328460-thx9zh11	72	19	solution	solution	NN
cord-328460-thx9zh11	72	20	is	be	VBZ
cord-328460-thx9zh11	72	21	about	about	RB
cord-328460-thx9zh11	72	22	40-fold	40-fold	CD
cord-328460-thx9zh11	72	23	faster	fast	RBR
cord-328460-thx9zh11	72	24	than	than	IN
cord-328460-thx9zh11	72	25	the	the	DT
cord-328460-thx9zh11	72	26	hybridization	hybridization	NN
cord-328460-thx9zh11	72	27	rate	rate	NN
cord-328460-thx9zh11	72	28	in	in	IN
cord-328460-thx9zh11	72	29	solid	solid	JJ
cord-328460-thx9zh11	72	30	-	-	HYPH
cord-328460-thx9zh11	72	31	liquid	liquid	JJ
cord-328460-thx9zh11	72	32	interfaces	interface	NNS
cord-328460-thx9zh11	72	33	such	such	JJ
cord-328460-thx9zh11	72	34	as	as	IN
cord-328460-thx9zh11	72	35	those	those	DT
cord-328460-thx9zh11	72	36	of	of	IN
cord-328460-thx9zh11	72	37	microarrays	microarray	NNS
cord-328460-thx9zh11	72	38	.	.	.
cord-328460-thx9zh11	73	1	The	the	DT
cord-328460-thx9zh11	73	2	typical	typical	JJ
cord-328460-thx9zh11	73	3	experimental	experimental	JJ
cord-328460-thx9zh11	73	4	configuration	configuration	NN
cord-328460-thx9zh11	73	5	of	of	IN
cord-328460-thx9zh11	73	6	devices	device	NNS
cord-328460-thx9zh11	73	7	performing	perform	VBG
cord-328460-thx9zh11	73	8	droplet	droplet	NN
cord-328460-thx9zh11	73	9	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	73	10	PCR	PCR	NNP
cord-328460-thx9zh11	73	11	amplification	amplification	NN
cord-328460-thx9zh11	73	12	includes	include	VBZ
cord-328460-thx9zh11	73	13	an	an	DT
cord-328460-thx9zh11	73	14	oil	oil	NN
cord-328460-thx9zh11	73	15	stream	stream	NN
cord-328460-thx9zh11	73	16	that	that	WDT
cord-328460-thx9zh11	73	17	cyclically	cyclically	RB
cord-328460-thx9zh11	73	18	drives	drive	VBZ
cord-328460-thx9zh11	73	19	droplets	droplet	NNS
cord-328460-thx9zh11	73	20	through	through	IN
cord-328460-thx9zh11	73	21	alternating	alternate	VBG
cord-328460-thx9zh11	73	22	temperature	temperature	NN
cord-328460-thx9zh11	73	23	zones	zone	NNS
cord-328460-thx9zh11	73	24	for	for	IN
cord-328460-thx9zh11	73	25	denaturation	denaturation	NN
cord-328460-thx9zh11	73	26	and	and	CC
cord-328460-thx9zh11	73	27	annealing	annealing	NN
cord-328460-thx9zh11	73	28	thus	thus	RB
cord-328460-thx9zh11	73	29	obtaining	obtain	VBG
cord-328460-thx9zh11	73	30	a	a	DT
cord-328460-thx9zh11	73	31	rapid	rapid	JJ
cord-328460-thx9zh11	73	32	and	and	CC
cord-328460-thx9zh11	73	33	efficient	efficient	JJ
cord-328460-thx9zh11	73	34	PCR	PCR	NNP
cord-328460-thx9zh11	73	35	thermocycling	thermocycle	VBG
cord-328460-thx9zh11	73	36	[	[	-LRB-
cord-328460-thx9zh11	73	37	38	38	CD
cord-328460-thx9zh11	73	38	]	]	-RRB-
cord-328460-thx9zh11	73	39	.	.	.
cord-328460-thx9zh11	74	1	The	the	DT
cord-328460-thx9zh11	74	2	implementation	implementation	NN
cord-328460-thx9zh11	74	3	of	of	IN
cord-328460-thx9zh11	74	4	PCR	PCR	NNP
cord-328460-thx9zh11	74	5	amplification	amplification	NN
cord-328460-thx9zh11	74	6	in	in	IN
cord-328460-thx9zh11	74	7	droplet	droplet	NN
cord-328460-thx9zh11	74	8	-	-	HYPH
cord-328460-thx9zh11	74	9	based	base	VBN
cord-328460-thx9zh11	74	10	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	74	11	systems	system	NNS
cord-328460-thx9zh11	74	12	[	[	-LRB-
cord-328460-thx9zh11	74	13	39	39	CD
cord-328460-thx9zh11	74	14	]	]	-RRB-
cord-328460-thx9zh11	74	15	benefits	benefit	NNS
cord-328460-thx9zh11	74	16	from	from	IN
cord-328460-thx9zh11	74	17	the	the	DT
cord-328460-thx9zh11	74	18	reduced	reduce	VBN
cord-328460-thx9zh11	74	19	reaction	reaction	NN
cord-328460-thx9zh11	74	20	volumes	volume	NNS
cord-328460-thx9zh11	74	21	that	that	WDT
cord-328460-thx9zh11	74	22	result	result	VBP
cord-328460-thx9zh11	74	23	in	in	IN
cord-328460-thx9zh11	74	24	a	a	DT
cord-328460-thx9zh11	74	25	decreased	decreased	JJ
cord-328460-thx9zh11	74	26	reagent	reagent	NN
cord-328460-thx9zh11	74	27	and	and	CC
cord-328460-thx9zh11	74	28	sample	sample	NN
cord-328460-thx9zh11	74	29	consumption	consumption	NN
cord-328460-thx9zh11	74	30	.	.	.
cord-328460-thx9zh11	75	1	The	the	DT
cord-328460-thx9zh11	75	2	possibility	possibility	NN
cord-328460-thx9zh11	75	3	of	of	IN
cord-328460-thx9zh11	75	4	lowering	lower	VBG
cord-328460-thx9zh11	75	5	the	the	DT
cord-328460-thx9zh11	75	6	sample	sample	NN
cord-328460-thx9zh11	75	7	requirement	requirement	NN
cord-328460-thx9zh11	75	8	is	be	VBZ
cord-328460-thx9zh11	75	9	particularly	particularly	RB
cord-328460-thx9zh11	75	10	important	important	JJ
cord-328460-thx9zh11	75	11	when	when	WRB
cord-328460-thx9zh11	75	12	limited	limited	JJ
cord-328460-thx9zh11	75	13	template	template	NN
cord-328460-thx9zh11	75	14	material	material	NN
cord-328460-thx9zh11	75	15	is	be	VBZ
cord-328460-thx9zh11	75	16	available	available	JJ
cord-328460-thx9zh11	75	17	for	for	IN
cord-328460-thx9zh11	75	18	the	the	DT
cord-328460-thx9zh11	75	19	analysis	analysis	NN
cord-328460-thx9zh11	75	20	[	[	-LRB-
cord-328460-thx9zh11	75	21	37	37	CD
cord-328460-thx9zh11	75	22	]	]	-RRB-
cord-328460-thx9zh11	75	23	.	.	.
cord-328460-thx9zh11	76	1	In	in	IN
cord-328460-thx9zh11	76	2	addition	addition	NN
cord-328460-thx9zh11	76	3	,	,	,
cord-328460-thx9zh11	76	4	the	the	DT
cord-328460-thx9zh11	76	5	use	use	NN
cord-328460-thx9zh11	76	6	of	of	IN
cord-328460-thx9zh11	76	7	droplet	droplet	NN
cord-328460-thx9zh11	76	8	-	-	HYPH
cord-328460-thx9zh11	76	9	based	base	VBN
cord-328460-thx9zh11	76	10	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	76	11	systems	system	NNS
cord-328460-thx9zh11	76	12	for	for	IN
cord-328460-thx9zh11	76	13	PCR	PCR	NNP
cord-328460-thx9zh11	76	14	enables	enable	VBZ
cord-328460-thx9zh11	76	15	the	the	DT
cord-328460-thx9zh11	76	16	amplification	amplification	NN
cord-328460-thx9zh11	76	17	and	and	CC
cord-328460-thx9zh11	76	18	the	the	DT
cord-328460-thx9zh11	76	19	analysis	analysis	NN
cord-328460-thx9zh11	76	20	of	of	IN
cord-328460-thx9zh11	76	21	individual	individual	JJ
cord-328460-thx9zh11	76	22	target	target	NN
cord-328460-thx9zh11	76	23	sequences	sequence	NNS
cord-328460-thx9zh11	76	24	to	to	TO
cord-328460-thx9zh11	76	25	be	be	VB
cord-328460-thx9zh11	76	26	performed	perform	VBN
cord-328460-thx9zh11	76	27	in	in	IN
cord-328460-thx9zh11	76	28	a	a	DT
cord-328460-thx9zh11	76	29	significantly	significantly	RB
cord-328460-thx9zh11	76	30	lower	low	JJR
cord-328460-thx9zh11	76	31	time	time	NN
cord-328460-thx9zh11	76	32	as	as	IN
cord-328460-thx9zh11	76	33	a	a	DT
cord-328460-thx9zh11	76	34	consequence	consequence	NN
cord-328460-thx9zh11	76	35	of	of	IN
cord-328460-thx9zh11	76	36	a	a	DT
cord-328460-thx9zh11	76	37	higher	high	JJR
cord-328460-thx9zh11	76	38	thermal	thermal	JJ
cord-328460-thx9zh11	76	39	cycling	cycling	NN
cord-328460-thx9zh11	76	40	speed	speed	NN
cord-328460-thx9zh11	76	41	.	.	.
cord-328460-thx9zh11	77	1	A	a	DT
cord-328460-thx9zh11	77	2	number	number	NN
cord-328460-thx9zh11	77	3	of	of	IN
cord-328460-thx9zh11	77	4	different	different	JJ
cord-328460-thx9zh11	77	5	isothermal	isothermal	JJ
cord-328460-thx9zh11	77	6	amplification	amplification	NN
cord-328460-thx9zh11	77	7	procedures	procedure	NNS
cord-328460-thx9zh11	77	8	have	have	VBP
cord-328460-thx9zh11	77	9	been	be	VBN
cord-328460-thx9zh11	77	10	introduced	introduce	VBN
cord-328460-thx9zh11	77	11	over	over	IN
cord-328460-thx9zh11	77	12	the	the	DT
cord-328460-thx9zh11	77	13	last	last	JJ
cord-328460-thx9zh11	77	14	10	10	CD
cord-328460-thx9zh11	77	15	years	year	NNS
cord-328460-thx9zh11	77	16	.	.	.
cord-328460-thx9zh11	78	1	These	these	DT
cord-328460-thx9zh11	78	2	include	include	VBP
cord-328460-thx9zh11	78	3	nucleic	nucleic	JJ
cord-328460-thx9zh11	78	4	acid	acid	NN
cord-328460-thx9zh11	78	5	sequence	sequence	NN
cord-328460-thx9zh11	78	6	-	-	HYPH
cord-328460-thx9zh11	78	7	based	base	VBN
cord-328460-thx9zh11	78	8	amplification	amplification	NN
cord-328460-thx9zh11	78	9	(	(	-LRB-
cord-328460-thx9zh11	78	10	NASBA	NASBA	NNP
cord-328460-thx9zh11	78	11	)	)	-RRB-
cord-328460-thx9zh11	78	12	,	,	,
cord-328460-thx9zh11	78	13	loop	loop	NN
cord-328460-thx9zh11	78	14	-	-	HYPH
cord-328460-thx9zh11	78	15	mediated	mediate	VBN
cord-328460-thx9zh11	78	16	amplification	amplification	NN
cord-328460-thx9zh11	78	17	(	(	-LRB-
cord-328460-thx9zh11	78	18	LAMP	LAMP	NNP
cord-328460-thx9zh11	78	19	)	)	-RRB-
cord-328460-thx9zh11	78	20	,	,	,
cord-328460-thx9zh11	78	21	helicase	helicase	NN
cord-328460-thx9zh11	78	22	-	-	HYPH
cord-328460-thx9zh11	78	23	dependent	dependent	JJ
cord-328460-thx9zh11	78	24	amplification	amplification	NN
cord-328460-thx9zh11	78	25	(	(	-LRB-
cord-328460-thx9zh11	78	26	HDA	HDA	NNP
cord-328460-thx9zh11	78	27	)	)	-RRB-
cord-328460-thx9zh11	78	28	,	,	,
cord-328460-thx9zh11	78	29	rolling	roll	VBG
cord-328460-thx9zh11	78	30	circle	circle	NN
cord-328460-thx9zh11	78	31	amplification	amplification	NN
cord-328460-thx9zh11	78	32	(	(	-LRB-
cord-328460-thx9zh11	78	33	RCA	RCA	NNP
cord-328460-thx9zh11	78	34	)	)	-RRB-
cord-328460-thx9zh11	78	35	,	,	,
cord-328460-thx9zh11	78	36	recombinase	recombinase	NNP
cord-328460-thx9zh11	78	37	polymerase	polymerase	NN
cord-328460-thx9zh11	78	38	amplification	amplification	NN
cord-328460-thx9zh11	78	39	(	(	-LRB-
cord-328460-thx9zh11	78	40	RPA	RPA	NNP
cord-328460-thx9zh11	78	41	)	)	-RRB-
cord-328460-thx9zh11	78	42	and	and	CC
cord-328460-thx9zh11	78	43	multiple	multiple	JJ
cord-328460-thx9zh11	78	44	displacement	displacement	NN
cord-328460-thx9zh11	78	45	amplification	amplification	NN
cord-328460-thx9zh11	78	46	(	(	-LRB-
cord-328460-thx9zh11	78	47	MDA	MDA	NNP
cord-328460-thx9zh11	78	48	)	)	-RRB-
cord-328460-thx9zh11	78	49	[	[	-LRB-
cord-328460-thx9zh11	78	50	7	7	CD
cord-328460-thx9zh11	78	51	]	]	-RRB-
cord-328460-thx9zh11	78	52	.	.	.
cord-328460-thx9zh11	79	1	Isothermal	isothermal	JJ
cord-328460-thx9zh11	79	2	methods	method	NNS
cord-328460-thx9zh11	79	3	differ	differ	VBP
cord-328460-thx9zh11	79	4	from	from	IN
cord-328460-thx9zh11	79	5	PCR	PCR	NNP
cord-328460-thx9zh11	79	6	in	in	IN
cord-328460-thx9zh11	79	7	that	that	IN
cord-328460-thx9zh11	79	8	there	there	EX
cord-328460-thx9zh11	79	9	is	be	VBZ
cord-328460-thx9zh11	79	10	no	no	DT
cord-328460-thx9zh11	79	11	need	need	NN
cord-328460-thx9zh11	79	12	for	for	IN
cord-328460-thx9zh11	79	13	temperature	temperature	NN
cord-328460-thx9zh11	79	14	cycling	cycling	NN
cord-328460-thx9zh11	79	15	or	or	CC
cord-328460-thx9zh11	79	16	rapid	rapid	JJ
cord-328460-thx9zh11	79	17	heating	heating	NN
cord-328460-thx9zh11	79	18	and	and	CC
cord-328460-thx9zh11	79	19	cooling	cooling	NN
cord-328460-thx9zh11	79	20	mechanisms	mechanism	NNS
cord-328460-thx9zh11	79	21	as	as	IN
cord-328460-thx9zh11	79	22	in	in	IN
cord-328460-thx9zh11	79	23	miniaturized	miniaturize	VBN
cord-328460-thx9zh11	79	24	PCR	PCR	NNP
cord-328460-thx9zh11	79	25	systems	system	NNS
cord-328460-thx9zh11	79	26	.	.	.
cord-328460-thx9zh11	80	1	Many	many	JJ
cord-328460-thx9zh11	80	2	miniaturized	miniaturize	VBD
cord-328460-thx9zh11	80	3	isothermal	isothermal	JJ
cord-328460-thx9zh11	80	4	amplification	amplification	NN
cord-328460-thx9zh11	80	5	systems	system	NNS
cord-328460-thx9zh11	80	6	exploit	exploit	VBP
cord-328460-thx9zh11	80	7	the	the	DT
cord-328460-thx9zh11	80	8	strand	strand	NN
cord-328460-thx9zh11	80	9	-	-	HYPH
cord-328460-thx9zh11	80	10	displacement	displacement	NN
cord-328460-thx9zh11	80	11	activity	activity	NN
cord-328460-thx9zh11	80	12	of	of	IN
cord-328460-thx9zh11	80	13	a	a	DT
cord-328460-thx9zh11	80	14	DNA	dna	NN
cord-328460-thx9zh11	80	15	polymerase	polymerase	NN
cord-328460-thx9zh11	80	16	to	to	TO
cord-328460-thx9zh11	80	17	cyclically	cyclically	RB
cord-328460-thx9zh11	80	18	amplify	amplify	VB
cord-328460-thx9zh11	80	19	a	a	DT
cord-328460-thx9zh11	80	20	target	target	NN
cord-328460-thx9zh11	80	21	in	in	IN
cord-328460-thx9zh11	80	22	less	less	JJR
cord-328460-thx9zh11	80	23	than	than	IN
cord-328460-thx9zh11	80	24	an	an	DT
cord-328460-thx9zh11	80	25	hour	hour	NN
cord-328460-thx9zh11	80	26	.	.	.
cord-328460-thx9zh11	81	1	Reactions	reaction	NNS
cord-328460-thx9zh11	81	2	take	take	VBP
cord-328460-thx9zh11	81	3	place	place	NN
cord-328460-thx9zh11	81	4	in	in	IN
cord-328460-thx9zh11	81	5	a	a	DT
cord-328460-thx9zh11	81	6	microchamber	microchamber	NN
cord-328460-thx9zh11	81	7	or	or	CC
cord-328460-thx9zh11	81	8	in	in	IN
cord-328460-thx9zh11	81	9	a	a	DT
cord-328460-thx9zh11	81	10	microchannel	microchannel	NN
cord-328460-thx9zh11	81	11	;	;	:
cord-328460-thx9zh11	81	12	reservoirs	reservoir	NNS
cord-328460-thx9zh11	81	13	can	can	MD
cord-328460-thx9zh11	81	14	be	be	VB
cord-328460-thx9zh11	81	15	included	include	VBN
cord-328460-thx9zh11	81	16	to	to	TO
cord-328460-thx9zh11	81	17	inject	inject	VB
cord-328460-thx9zh11	81	18	reagents	reagent	NNS
cord-328460-thx9zh11	81	19	necessary	necessary	JJ
cord-328460-thx9zh11	81	20	for	for	IN
cord-328460-thx9zh11	81	21	reactions	reaction	NNS
cord-328460-thx9zh11	81	22	and	and	CC
cord-328460-thx9zh11	81	23	to	to	TO
cord-328460-thx9zh11	81	24	mix	mix	VB
cord-328460-thx9zh11	81	25	them	-PRON-	PRP
cord-328460-thx9zh11	81	26	with	with	IN
cord-328460-thx9zh11	81	27	the	the	DT
cord-328460-thx9zh11	81	28	sample	sample	NN
cord-328460-thx9zh11	81	29	.	.	.
cord-328460-thx9zh11	82	1	Primers	primer	NNS
cord-328460-thx9zh11	82	2	,	,	,
cord-328460-thx9zh11	82	3	enzymes	enzyme	NNS
cord-328460-thx9zh11	82	4	and	and	CC
cord-328460-thx9zh11	82	5	reagents	reagent	NNS
cord-328460-thx9zh11	82	6	can	can	MD
cord-328460-thx9zh11	82	7	also	also	RB
cord-328460-thx9zh11	82	8	be	be	VB
cord-328460-thx9zh11	82	9	stored	store	VBN
cord-328460-thx9zh11	82	10	in	in	IN
cord-328460-thx9zh11	82	11	the	the	DT
cord-328460-thx9zh11	82	12	chip	chip	NN
cord-328460-thx9zh11	82	13	and	and	CC
cord-328460-thx9zh11	82	14	,	,	,
cord-328460-thx9zh11	82	15	in	in	IN
cord-328460-thx9zh11	82	16	order	order	NN
cord-328460-thx9zh11	82	17	to	to	TO
cord-328460-thx9zh11	82	18	obtain	obtain	VB
cord-328460-thx9zh11	82	19	multiplex	multiplex	JJ
cord-328460-thx9zh11	82	20	analyses	analysis	NNS
cord-328460-thx9zh11	82	21	,	,	,
cord-328460-thx9zh11	82	22	different	different	JJ
cord-328460-thx9zh11	82	23	primers	primer	NNS
cord-328460-thx9zh11	82	24	for	for	IN
cord-328460-thx9zh11	82	25	several	several	JJ
cord-328460-thx9zh11	82	26	targets	target	NNS
cord-328460-thx9zh11	82	27	can	can	MD
cord-328460-thx9zh11	82	28	be	be	VB
cord-328460-thx9zh11	82	29	employed	employ	VBN
cord-328460-thx9zh11	82	30	.	.	.
cord-328460-thx9zh11	83	1	Loop	loop	NN
cord-328460-thx9zh11	83	2	-	-	HYPH
cord-328460-thx9zh11	83	3	mediated	mediate	VBN
cord-328460-thx9zh11	83	4	isothermal	isothermal	JJ
cord-328460-thx9zh11	83	5	amplification	amplification	NN
cord-328460-thx9zh11	83	6	(	(	-LRB-
cord-328460-thx9zh11	83	7	LAMP	LAMP	NNP
cord-328460-thx9zh11	83	8	)	)	-RRB-
cord-328460-thx9zh11	83	9	,	,	,
cord-328460-thx9zh11	83	10	described	describe	VBN
cord-328460-thx9zh11	83	11	for	for	IN
cord-328460-thx9zh11	83	12	the	the	DT
cord-328460-thx9zh11	83	13	first	first	JJ
cord-328460-thx9zh11	83	14	time	time	NN
cord-328460-thx9zh11	83	15	by	by	IN
cord-328460-thx9zh11	83	16	Notomi	Notomi	NNP
cord-328460-thx9zh11	83	17	et	et	NNP
cord-328460-thx9zh11	83	18	al	al	NNP
cord-328460-thx9zh11	83	19	.	.	.
cord-328460-thx9zh11	84	1	in	in	IN
cord-328460-thx9zh11	84	2	2000	2000	CD
cord-328460-thx9zh11	84	3	[	[	-LRB-
cord-328460-thx9zh11	84	4	40	40	CD
cord-328460-thx9zh11	84	5	]	]	-RRB-
cord-328460-thx9zh11	84	6	,	,	,
cord-328460-thx9zh11	84	7	utilizes	utilize	VBZ
cord-328460-thx9zh11	84	8	two	two	CD
cord-328460-thx9zh11	84	9	sets	set	NNS
cord-328460-thx9zh11	84	10	of	of	IN
cord-328460-thx9zh11	84	11	specially	specially	RB
cord-328460-thx9zh11	84	12	designed	design	VBN
cord-328460-thx9zh11	84	13	primers	primer	NNS
cord-328460-thx9zh11	84	14	,	,	,
cord-328460-thx9zh11	84	15	termed	term	VBN
cord-328460-thx9zh11	84	16	inner	inner	JJ
cord-328460-thx9zh11	84	17	and	and	CC
cord-328460-thx9zh11	84	18	outer	outer	JJ
cord-328460-thx9zh11	84	19	primers	primer	NNS
cord-328460-thx9zh11	84	20	,	,	,
cord-328460-thx9zh11	84	21	and	and	CC
cord-328460-thx9zh11	84	22	a	a	DT
cord-328460-thx9zh11	84	23	DNA	dna	NN
cord-328460-thx9zh11	84	24	polymerase	polymerase	NN
cord-328460-thx9zh11	84	25	with	with	IN
cord-328460-thx9zh11	84	26	strand	strand	NN
cord-328460-thx9zh11	84	27	displacement	displacement	NN
cord-328460-thx9zh11	84	28	activity	activity	NN
cord-328460-thx9zh11	84	29	.	.	.
cord-328460-thx9zh11	85	1	LAMP	LAMP	NNP
cord-328460-thx9zh11	85	2	recognizes	recognize	VBZ
cord-328460-thx9zh11	85	3	the	the	DT
cord-328460-thx9zh11	85	4	target	target	NN
cord-328460-thx9zh11	85	5	sequence	sequence	NN
cord-328460-thx9zh11	85	6	and	and	CC
cord-328460-thx9zh11	85	7	generates	generate	VBZ
cord-328460-thx9zh11	85	8	a	a	DT
cord-328460-thx9zh11	85	9	large	large	JJ
cord-328460-thx9zh11	85	10	amount	amount	NN
cord-328460-thx9zh11	85	11	of	of	IN
cord-328460-thx9zh11	85	12	amplified	amplified	JJ
cord-328460-thx9zh11	85	13	product	product	NN
cord-328460-thx9zh11	85	14	within	within	IN
cord-328460-thx9zh11	85	15	1	1	CD
cord-328460-thx9zh11	85	16	h.	h.	NN
cord-328460-thx9zh11	86	1	It	-PRON-	PRP
cord-328460-thx9zh11	86	2	has	have	VBZ
cord-328460-thx9zh11	86	3	been	be	VBN
cord-328460-thx9zh11	86	4	widely	widely	RB
cord-328460-thx9zh11	86	5	applied	apply	VBN
cord-328460-thx9zh11	86	6	for	for	IN
cord-328460-thx9zh11	86	7	the	the	DT
cord-328460-thx9zh11	86	8	detection	detection	NN
cord-328460-thx9zh11	86	9	of	of	IN
cord-328460-thx9zh11	86	10	pathogens	pathogen	NNS
cord-328460-thx9zh11	86	11	such	such	JJ
cord-328460-thx9zh11	86	12	as	as	IN
cord-328460-thx9zh11	86	13	human	human	JJ
cord-328460-thx9zh11	86	14	immunodeficiency	immunodeficiency	NN
cord-328460-thx9zh11	86	15	virus	virus	NN
cord-328460-thx9zh11	86	16	(	(	-LRB-
cord-328460-thx9zh11	86	17	HIV	HIV	NNP
cord-328460-thx9zh11	86	18	)	)	-RRB-
cord-328460-thx9zh11	86	19	[	[	-LRB-
cord-328460-thx9zh11	86	20	41	41	CD
cord-328460-thx9zh11	86	21	]	]	-RRB-
cord-328460-thx9zh11	86	22	,	,	,
cord-328460-thx9zh11	86	23	severe	severe	JJ
cord-328460-thx9zh11	86	24	acute	acute	JJ
cord-328460-thx9zh11	86	25	respiratory	respiratory	JJ
cord-328460-thx9zh11	86	26	syndrome	syndrome	NN
cord-328460-thx9zh11	86	27	coronavirus	coronavirus	NN
cord-328460-thx9zh11	86	28	(	(	-LRB-
cord-328460-thx9zh11	86	29	SARS	SARS	NNP
cord-328460-thx9zh11	86	30	-	-	HYPH
cord-328460-thx9zh11	86	31	CoV	CoV	NNP
cord-328460-thx9zh11	86	32	)	)	-RRB-
cord-328460-thx9zh11	86	33	[	[	-LRB-
cord-328460-thx9zh11	86	34	42	42	CD
cord-328460-thx9zh11	86	35	]	]	-RRB-
cord-328460-thx9zh11	86	36	,	,	,
cord-328460-thx9zh11	86	37	Staphylococcus	Staphylococcus	NNP
cord-328460-thx9zh11	86	38	aureus	aureus	NN
cord-328460-thx9zh11	86	39	[	[	-LRB-
cord-328460-thx9zh11	86	40	43	43	CD
cord-328460-thx9zh11	86	41	]	]	-RRB-
cord-328460-thx9zh11	86	42	,	,	,
cord-328460-thx9zh11	86	43	Salmonella	Salmonella	NNP
cord-328460-thx9zh11	86	44	enterica	enterica	NN
cord-328460-thx9zh11	86	45	[	[	-LRB-
cord-328460-thx9zh11	86	46	44	44	CD
cord-328460-thx9zh11	86	47	]	]	-RRB-
cord-328460-thx9zh11	86	48	.	.	.
cord-328460-thx9zh11	87	1	LAMP	lamp	NN
cord-328460-thx9zh11	87	2	reaction	reaction	NN
cord-328460-thx9zh11	87	3	is	be	VBZ
cord-328460-thx9zh11	87	4	initiated	initiate	VBN
cord-328460-thx9zh11	87	5	by	by	IN
cord-328460-thx9zh11	87	6	a	a	DT
cord-328460-thx9zh11	87	7	forward	forward	JJ
cord-328460-thx9zh11	87	8	inner	inner	JJ
cord-328460-thx9zh11	87	9	primer	primer	NN
cord-328460-thx9zh11	87	10	(	(	-LRB-
cord-328460-thx9zh11	87	11	FIP	FIP	NNP
cord-328460-thx9zh11	87	12	)	)	-RRB-
cord-328460-thx9zh11	87	13	,	,	,
cord-328460-thx9zh11	87	14	containing	contain	VBG
cord-328460-thx9zh11	87	15	sequences	sequence	NNS
cord-328460-thx9zh11	87	16	of	of	IN
cord-328460-thx9zh11	87	17	the	the	DT
cord-328460-thx9zh11	87	18	sense	sense	NN
cord-328460-thx9zh11	87	19	strand	strand	NN
cord-328460-thx9zh11	87	20	of	of	IN
cord-328460-thx9zh11	87	21	the	the	DT
cord-328460-thx9zh11	87	22	target	target	NN
cord-328460-thx9zh11	87	23	DNA	dna	NN
cord-328460-thx9zh11	87	24	,	,	,
cord-328460-thx9zh11	87	25	which	which	WDT
cord-328460-thx9zh11	87	26	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	87	27	to	to	IN
cord-328460-thx9zh11	87	28	F2c	f2c	NN
cord-328460-thx9zh11	87	29	in	in	IN
cord-328460-thx9zh11	87	30	the	the	DT
cord-328460-thx9zh11	87	31	target	target	NN
cord-328460-thx9zh11	87	32	and	and	CC
cord-328460-thx9zh11	87	33	initiates	initiate	VBZ
cord-328460-thx9zh11	87	34	complementary	complementary	JJ
cord-328460-thx9zh11	87	35	strand	strand	NN
cord-328460-thx9zh11	87	36	synthesis	synthesis	NN
cord-328460-thx9zh11	87	37	(	(	-LRB-
cord-328460-thx9zh11	87	38	Figure	figure	NN
cord-328460-thx9zh11	87	39	1	1	CD
cord-328460-thx9zh11	87	40	)	)	-RRB-
cord-328460-thx9zh11	87	41	.	.	.
cord-328460-thx9zh11	88	1	Then	then	RB
cord-328460-thx9zh11	88	2	,	,	,
cord-328460-thx9zh11	88	3	the	the	DT
cord-328460-thx9zh11	88	4	outer	outer	JJ
cord-328460-thx9zh11	88	5	primer	primer	NN
cord-328460-thx9zh11	88	6	F3	F3	NNP
cord-328460-thx9zh11	88	7	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	88	8	to	to	IN
cord-328460-thx9zh11	88	9	F3c	F3c	NNP
cord-328460-thx9zh11	88	10	portion	portion	NN
cord-328460-thx9zh11	88	11	in	in	IN
cord-328460-thx9zh11	88	12	the	the	DT
cord-328460-thx9zh11	88	13	target	target	NN
cord-328460-thx9zh11	88	14	sequence	sequence	NN
cord-328460-thx9zh11	88	15	leading	lead	VBG
cord-328460-thx9zh11	88	16	to	to	IN
cord-328460-thx9zh11	88	17	the	the	DT
cord-328460-thx9zh11	88	18	displacement	displacement	NN
cord-328460-thx9zh11	88	19	of	of	IN
cord-328460-thx9zh11	88	20	the	the	DT
cord-328460-thx9zh11	88	21	just	just	RB
cord-328460-thx9zh11	88	22	synthesized	synthesize	VBN
cord-328460-thx9zh11	88	23	strand	strand	NN
cord-328460-thx9zh11	88	24	that	that	WDT
cord-328460-thx9zh11	88	25	is	be	VBZ
cord-328460-thx9zh11	88	26	release	release	NN
cord-328460-thx9zh11	88	27	as	as	IN
cord-328460-thx9zh11	88	28	a	a	DT
cord-328460-thx9zh11	88	29	single	single	RB
cord-328460-thx9zh11	88	30	-	-	HYPH
cord-328460-thx9zh11	88	31	stranded	strand	VBN
cord-328460-thx9zh11	88	32	DNA	dna	NN
cord-328460-thx9zh11	88	33	with	with	IN
cord-328460-thx9zh11	88	34	a	a	DT
cord-328460-thx9zh11	88	35	loop	loop	NN
cord-328460-thx9zh11	88	36	out	out	RP
cord-328460-thx9zh11	88	37	structure	structure	NN
cord-328460-thx9zh11	88	38	at	at	IN
cord-328460-thx9zh11	88	39	one	one	CD
cord-328460-thx9zh11	88	40	end	end	NN
cord-328460-thx9zh11	88	41	.	.	.
cord-328460-thx9zh11	89	1	The	the	DT
cord-328460-thx9zh11	89	2	FIP	FIP	NNP
cord-328460-thx9zh11	89	3	-	-	HYPH
cord-328460-thx9zh11	89	4	linked	link	VBN
cord-328460-thx9zh11	89	5	complementary	complementary	JJ
cord-328460-thx9zh11	89	6	strand	strand	NN
cord-328460-thx9zh11	89	7	acts	act	NNS
cord-328460-thx9zh11	89	8	as	as	IN
cord-328460-thx9zh11	89	9	the	the	DT
cord-328460-thx9zh11	89	10	template	template	NN
cord-328460-thx9zh11	89	11	for	for	IN
cord-328460-thx9zh11	89	12	a	a	DT
cord-328460-thx9zh11	89	13	new	new	JJ
cord-328460-thx9zh11	89	14	DNA	dna	NN
cord-328460-thx9zh11	89	15	synthesis	synthesis	NN
cord-328460-thx9zh11	89	16	primed	prime	VBN
cord-328460-thx9zh11	89	17	by	by	IN
cord-328460-thx9zh11	89	18	backward	backward	JJ
cord-328460-thx9zh11	89	19	inner	inner	NN
cord-328460-thx9zh11	89	20	(	(	-LRB-
cord-328460-thx9zh11	89	21	BIP	BIP	NNP
cord-328460-thx9zh11	89	22	)	)	-RRB-
cord-328460-thx9zh11	89	23	and	and	CC
cord-328460-thx9zh11	89	24	outer	outer	JJ
cord-328460-thx9zh11	89	25	(	(	-LRB-
cord-328460-thx9zh11	89	26	B3	b3	NN
cord-328460-thx9zh11	89	27	)	)	-RRB-
cord-328460-thx9zh11	89	28	primers	primer	NNS
cord-328460-thx9zh11	89	29	that	that	WDT
cord-328460-thx9zh11	89	30	hybridize	hybridize	VBP
cord-328460-thx9zh11	89	31	to	to	IN
cord-328460-thx9zh11	89	32	the	the	DT
cord-328460-thx9zh11	89	33	other	other	JJ
cord-328460-thx9zh11	89	34	end	end	NN
cord-328460-thx9zh11	89	35	of	of	IN
cord-328460-thx9zh11	89	36	the	the	DT
cord-328460-thx9zh11	89	37	target	target	NN
cord-328460-thx9zh11	89	38	,	,	,
cord-328460-thx9zh11	89	39	leading	lead	VBG
cord-328460-thx9zh11	89	40	to	to	IN
cord-328460-thx9zh11	89	41	the	the	DT
cord-328460-thx9zh11	89	42	production	production	NN
cord-328460-thx9zh11	89	43	of	of	IN
cord-328460-thx9zh11	89	44	a	a	DT
cord-328460-thx9zh11	89	45	dumb	dumb	JJ
cord-328460-thx9zh11	89	46	-	-	HYPH
cord-328460-thx9zh11	89	47	bell	bell	JJ
cord-328460-thx9zh11	89	48	form	form	NN
cord-328460-thx9zh11	89	49	DNA	DNA	NNP
cord-328460-thx9zh11	89	50	which	which	WDT
cord-328460-thx9zh11	89	51	produces	produce	VBZ
cord-328460-thx9zh11	89	52	a	a	DT
cord-328460-thx9zh11	89	53	stem	stem	NN
cord-328460-thx9zh11	89	54	-	-	HYPH
cord-328460-thx9zh11	89	55	loop	loop	NN
cord-328460-thx9zh11	89	56	DNA	dna	NN
cord-328460-thx9zh11	89	57	structure	structure	NN
cord-328460-thx9zh11	89	58	as	as	IN
cord-328460-thx9zh11	89	59	a	a	DT
cord-328460-thx9zh11	89	60	consequence	consequence	NN
cord-328460-thx9zh11	89	61	of	of	IN
cord-328460-thx9zh11	89	62	self	self	NN
cord-328460-thx9zh11	89	63	-	-	HYPH
cord-328460-thx9zh11	89	64	primed	prime	VBN
cord-328460-thx9zh11	89	65	DNA	dna	NN
cord-328460-thx9zh11	89	66	synthesis	synthesis	NN
cord-328460-thx9zh11	89	67	.	.	.
cord-328460-thx9zh11	90	1	The	the	DT
cord-328460-thx9zh11	90	2	subsequent	subsequent	JJ
cord-328460-thx9zh11	90	3	cycles	cycle	NNS
cord-328460-thx9zh11	90	4	,	,	,
cord-328460-thx9zh11	90	5	comprising	comprise	VBG
cord-328460-thx9zh11	90	6	elongation	elongation	NN
cord-328460-thx9zh11	90	7	and	and	CC
cord-328460-thx9zh11	90	8	recycling	recycling	NN
cord-328460-thx9zh11	90	9	steps	step	NNS
cord-328460-thx9zh11	90	10	,	,	,
cord-328460-thx9zh11	90	11	lead	lead	VB
cord-328460-thx9zh11	90	12	to	to	IN
cord-328460-thx9zh11	90	13	final	final	JJ
cord-328460-thx9zh11	90	14	products	product	NNS
cord-328460-thx9zh11	90	15	constituted	constitute	VBN
cord-328460-thx9zh11	90	16	by	by	IN
cord-328460-thx9zh11	90	17	a	a	DT
cord-328460-thx9zh11	90	18	mixture	mixture	NN
cord-328460-thx9zh11	90	19	of	of	IN
cord-328460-thx9zh11	90	20	stem	stem	NN
cord-328460-thx9zh11	90	21	-	-	HYPH
cord-328460-thx9zh11	90	22	loop	loop	NN
cord-328460-thx9zh11	90	23	DNAs	dna	NNS
cord-328460-thx9zh11	90	24	having	have	VBG
cord-328460-thx9zh11	90	25	various	various	JJ
cord-328460-thx9zh11	90	26	stem	stem	NN
cord-328460-thx9zh11	90	27	lengths	length	NNS
cord-328460-thx9zh11	90	28	and	and	CC
cord-328460-thx9zh11	90	29	cauliflower	cauliflower	NN
cord-328460-thx9zh11	90	30	-	-	HYPH
cord-328460-thx9zh11	90	31	like	like	JJ
cord-328460-thx9zh11	90	32	structures	structure	NNS
cord-328460-thx9zh11	90	33	with	with	IN
cord-328460-thx9zh11	90	34	multiple	multiple	JJ
cord-328460-thx9zh11	90	35	loops	loop	NNS
cord-328460-thx9zh11	90	36	formed	form	VBN
cord-328460-thx9zh11	90	37	after	after	IN
cord-328460-thx9zh11	90	38	the	the	DT
cord-328460-thx9zh11	90	39	annealing	annealing	NN
cord-328460-thx9zh11	90	40	between	between	IN
cord-328460-thx9zh11	90	41	alternately	alternately	RB
cord-328460-thx9zh11	90	42	inverted	invert	VBN
cord-328460-thx9zh11	90	43	repeats	repeat	NNS
cord-328460-thx9zh11	90	44	of	of	IN
cord-328460-thx9zh11	90	45	the	the	DT
cord-328460-thx9zh11	90	46	target	target	NN
cord-328460-thx9zh11	90	47	sequence	sequence	NN
cord-328460-thx9zh11	90	48	in	in	IN
cord-328460-thx9zh11	90	49	the	the	DT
cord-328460-thx9zh11	90	50	same	same	JJ
cord-328460-thx9zh11	90	51	strand	strand	NN
cord-328460-thx9zh11	90	52	.	.	.
cord-328460-thx9zh11	91	1	It	-PRON-	PRP
cord-328460-thx9zh11	91	2	is	be	VBZ
cord-328460-thx9zh11	91	3	to	to	TO
cord-328460-thx9zh11	91	4	underline	underline	VB
cord-328460-thx9zh11	91	5	that	that	IN
cord-328460-thx9zh11	91	6	only	only	JJ
cord-328460-thx9zh11	91	7	inner	inner	JJ
cord-328460-thx9zh11	91	8	primers	primer	NNS
cord-328460-thx9zh11	91	9	are	be	VBP
cord-328460-thx9zh11	91	10	used	use	VBN
cord-328460-thx9zh11	91	11	for	for	IN
cord-328460-thx9zh11	91	12	strand	strand	NN
cord-328460-thx9zh11	91	13	displacement	displacement	NN
cord-328460-thx9zh11	91	14	DNA	dna	NN
cord-328460-thx9zh11	91	15	synthesis	synthesis	NN
cord-328460-thx9zh11	91	16	during	during	IN
cord-328460-thx9zh11	91	17	cycling	cycling	NN
cord-328460-thx9zh11	91	18	reactions	reaction	NNS
cord-328460-thx9zh11	91	19	while	while	IN
cord-328460-thx9zh11	91	20	all	all	DT
cord-328460-thx9zh11	91	21	four	four	CD
cord-328460-thx9zh11	91	22	primers	primer	NNS
cord-328460-thx9zh11	91	23	are	be	VBP
cord-328460-thx9zh11	91	24	used	use	VBN
cord-328460-thx9zh11	91	25	during	during	IN
cord-328460-thx9zh11	91	26	initial	initial	JJ
cord-328460-thx9zh11	91	27	steps	step	NNS
cord-328460-thx9zh11	91	28	of	of	IN
cord-328460-thx9zh11	91	29	the	the	DT
cord-328460-thx9zh11	91	30	LAMP	LAMP	NNP
cord-328460-thx9zh11	91	31	reaction	reaction	NN
cord-328460-thx9zh11	91	32	.	.	.
cord-328460-thx9zh11	92	1	The	the	DT
cord-328460-thx9zh11	92	2	initial	initial	JJ
cord-328460-thx9zh11	92	3	use	use	NN
cord-328460-thx9zh11	92	4	of	of	IN
cord-328460-thx9zh11	92	5	four	four	CD
cord-328460-thx9zh11	92	6	primers	primer	NNS
cord-328460-thx9zh11	92	7	that	that	WDT
cord-328460-thx9zh11	92	8	enables	enable	VBZ
cord-328460-thx9zh11	92	9	the	the	DT
cord-328460-thx9zh11	92	10	recognition	recognition	NN
cord-328460-thx9zh11	92	11	of	of	IN
cord-328460-thx9zh11	92	12	six	six	CD
cord-328460-thx9zh11	92	13	distinct	distinct	JJ
cord-328460-thx9zh11	92	14	sequences	sequence	NNS
cord-328460-thx9zh11	92	15	,	,	,
cord-328460-thx9zh11	92	16	followed	follow	VBN
cord-328460-thx9zh11	92	17	by	by	IN
cord-328460-thx9zh11	92	18	the	the	DT
cord-328460-thx9zh11	92	19	use	use	NN
cord-328460-thx9zh11	92	20	of	of	IN
cord-328460-thx9zh11	92	21	two	two	CD
cord-328460-thx9zh11	92	22	primers	primer	NNS
cord-328460-thx9zh11	92	23	,	,	,
cord-328460-thx9zh11	92	24	ensures	ensure	VBZ
cord-328460-thx9zh11	92	25	the	the	DT
cord-328460-thx9zh11	92	26	high	high	JJ
cord-328460-thx9zh11	92	27	selectivity	selectivity	NN
cord-328460-thx9zh11	92	28	for	for	IN
cord-328460-thx9zh11	92	29	target	target	NN
cord-328460-thx9zh11	92	30	amplification	amplification	NN
cord-328460-thx9zh11	92	31	.	.	.
cord-328460-thx9zh11	93	1	The	the	DT
cord-328460-thx9zh11	93	2	LAMP	lamp	NN
cord-328460-thx9zh11	93	3	method	method	NN
cord-328460-thx9zh11	93	4	does	do	VBZ
cord-328460-thx9zh11	93	5	not	not	RB
cord-328460-thx9zh11	93	6	require	require	VB
cord-328460-thx9zh11	93	7	thermocycling	thermocycle	VBG
cord-328460-thx9zh11	93	8	since	since	IN
cord-328460-thx9zh11	93	9	the	the	DT
cord-328460-thx9zh11	93	10	amplification	amplification	NN
cord-328460-thx9zh11	93	11	is	be	VBZ
cord-328460-thx9zh11	93	12	performed	perform	VBN
cord-328460-thx9zh11	93	13	at	at	IN
cord-328460-thx9zh11	93	14	a	a	DT
cord-328460-thx9zh11	93	15	constant	constant	JJ
cord-328460-thx9zh11	93	16	temperature	temperature	NN
cord-328460-thx9zh11	93	17	between	between	IN
cord-328460-thx9zh11	93	18	60	60	CD
cord-328460-thx9zh11	93	19	°	°	NN
cord-328460-thx9zh11	93	20	C	c	NN
cord-328460-thx9zh11	93	21	and	and	CC
cord-328460-thx9zh11	93	22	65	65	CD
cord-328460-thx9zh11	93	23	°	°	NN
cord-328460-thx9zh11	93	24	C	c	NN
cord-328460-thx9zh11	93	25	.	.	.
cord-328460-thx9zh11	94	1	Another	another	DT
cord-328460-thx9zh11	94	2	important	important	JJ
cord-328460-thx9zh11	94	3	advantage	advantage	NN
cord-328460-thx9zh11	94	4	of	of	IN
cord-328460-thx9zh11	94	5	the	the	DT
cord-328460-thx9zh11	94	6	LAMP	LAMP	NNP
cord-328460-thx9zh11	94	7	method	method	NN
cord-328460-thx9zh11	94	8	is	be	VBZ
cord-328460-thx9zh11	94	9	its	-PRON-	PRP$
cord-328460-thx9zh11	94	10	simplicity	simplicity	NN
cord-328460-thx9zh11	94	11	for	for	IN
cord-328460-thx9zh11	94	12	detection	detection	NN
cord-328460-thx9zh11	94	13	of	of	IN
cord-328460-thx9zh11	94	14	the	the	DT
cord-328460-thx9zh11	94	15	amplification	amplification	NN
cord-328460-thx9zh11	94	16	reaction	reaction	NN
cord-328460-thx9zh11	94	17	.	.	.
cord-328460-thx9zh11	95	1	In	in	IN
cord-328460-thx9zh11	95	2	fact	fact	NN
cord-328460-thx9zh11	95	3	,	,	,
cord-328460-thx9zh11	95	4	pyrophosphate	pyrophosphate	NN
cord-328460-thx9zh11	95	5	byproducts	byproduct	NNS
cord-328460-thx9zh11	95	6	are	be	VBP
cord-328460-thx9zh11	95	7	produced	produce	VBN
cord-328460-thx9zh11	95	8	during	during	IN
cord-328460-thx9zh11	95	9	the	the	DT
cord-328460-thx9zh11	95	10	reaction	reaction	NN
cord-328460-thx9zh11	95	11	.	.	.
cord-328460-thx9zh11	96	1	These	these	DT
cord-328460-thx9zh11	96	2	byproducts	byproduct	NNS
cord-328460-thx9zh11	96	3	form	form	VBP
cord-328460-thx9zh11	96	4	a	a	DT
cord-328460-thx9zh11	96	5	white	white	JJ
cord-328460-thx9zh11	96	6	precipitate	precipitate	NN
cord-328460-thx9zh11	96	7	that	that	WDT
cord-328460-thx9zh11	96	8	increases	increase	VBZ
cord-328460-thx9zh11	96	9	the	the	DT
cord-328460-thx9zh11	96	10	turbidity	turbidity	NN
cord-328460-thx9zh11	96	11	of	of	IN
cord-328460-thx9zh11	96	12	the	the	DT
cord-328460-thx9zh11	96	13	solution	solution	NN
cord-328460-thx9zh11	96	14	.	.	.
cord-328460-thx9zh11	97	1	The	the	DT
cord-328460-thx9zh11	97	2	detection	detection	NN
cord-328460-thx9zh11	97	3	of	of	IN
cord-328460-thx9zh11	97	4	amplification	amplification	NN
cord-328460-thx9zh11	97	5	products	product	NNS
cord-328460-thx9zh11	97	6	can	can	MD
cord-328460-thx9zh11	97	7	be	be	VB
cord-328460-thx9zh11	97	8	obtained	obtain	VBN
cord-328460-thx9zh11	97	9	by	by	IN
cord-328460-thx9zh11	97	10	using	use	VBG
cord-328460-thx9zh11	97	11	a	a	DT
cord-328460-thx9zh11	97	12	variety	variety	NN
cord-328460-thx9zh11	97	13	of	of	IN
cord-328460-thx9zh11	97	14	methods	method	NNS
cord-328460-thx9zh11	97	15	Although	although	IN
cord-328460-thx9zh11	97	16	most	most	JJS
cord-328460-thx9zh11	97	17	analytical	analytical	JJ
cord-328460-thx9zh11	97	18	tools	tool	NNS
cord-328460-thx9zh11	97	19	rely	rely	VBP
cord-328460-thx9zh11	97	20	on	on	IN
cord-328460-thx9zh11	97	21	bulk	bulk	JJ
cord-328460-thx9zh11	97	22	measurements	measurement	NNS
cord-328460-thx9zh11	97	23	of	of	IN
cord-328460-thx9zh11	97	24	LAMP	lamp	NN
cord-328460-thx9zh11	97	25	amplification	amplification	NN
cord-328460-thx9zh11	97	26	,	,	,
cord-328460-thx9zh11	97	27	LAMP	LAMP	NNP
cord-328460-thx9zh11	97	28	has	have	VBZ
cord-328460-thx9zh11	97	29	also	also	RB
cord-328460-thx9zh11	97	30	been	be	VBN
cord-328460-thx9zh11	97	31	down	down	RB
cord-328460-thx9zh11	97	32	-	-	HYPH
cord-328460-thx9zh11	97	33	scaled	scale	VBN
cord-328460-thx9zh11	97	34	by	by	IN
cord-328460-thx9zh11	97	35	integrating	integrate	VBG
cord-328460-thx9zh11	97	36	it	-PRON-	PRP
cord-328460-thx9zh11	97	37	in	in	IN
cord-328460-thx9zh11	97	38	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	97	39	devices	device	NNS
cord-328460-thx9zh11	97	40	.	.	.
cord-328460-thx9zh11	98	1	Lee	Lee	NNP
cord-328460-thx9zh11	98	2	et	et	NNP
cord-328460-thx9zh11	98	3	al	al	NNP
cord-328460-thx9zh11	98	4	.	.	NNP
cord-328460-thx9zh11	98	5	have	have	VBP
cord-328460-thx9zh11	98	6	developed	develop	VBN
cord-328460-thx9zh11	98	7	an	an	DT
cord-328460-thx9zh11	98	8	integrated	integrated	JJ
cord-328460-thx9zh11	98	9	isothermal	isothermal	JJ
cord-328460-thx9zh11	98	10	device	device	NN
cord-328460-thx9zh11	98	11	for	for	IN
cord-328460-thx9zh11	98	12	both	both	CC
cord-328460-thx9zh11	98	13	amplification	amplification	NN
cord-328460-thx9zh11	98	14	and	and	CC
cord-328460-thx9zh11	98	15	real	real	JJ
cord-328460-thx9zh11	98	16	-	-	HYPH
cord-328460-thx9zh11	98	17	time	time	NN
cord-328460-thx9zh11	98	18	detection	detection	NN
cord-328460-thx9zh11	98	19	of	of	IN
cord-328460-thx9zh11	98	20	hepatitis	hepatitis	NN
cord-328460-thx9zh11	98	21	B	B	NNP
cord-328460-thx9zh11	98	22	virus	virus	NN
cord-328460-thx9zh11	98	23	(	(	-LRB-
cord-328460-thx9zh11	98	24	HBV	HBV	NNP
cord-328460-thx9zh11	98	25	)	)	-RRB-
cord-328460-thx9zh11	98	26	DNA	DNA	NNP
cord-328460-thx9zh11	98	27	via	via	IN
cord-328460-thx9zh11	98	28	the	the	DT
cord-328460-thx9zh11	98	29	LAMP	lamp	NN
cord-328460-thx9zh11	98	30	amplification	amplification	NN
cord-328460-thx9zh11	98	31	method	method	NN
cord-328460-thx9zh11	98	32	[	[	-LRB-
cord-328460-thx9zh11	98	33	50	50	CD
cord-328460-thx9zh11	98	34	]	]	-RRB-
cord-328460-thx9zh11	98	35	.	.	.
cord-328460-thx9zh11	99	1	The	the	DT
cord-328460-thx9zh11	99	2	device	device	NN
cord-328460-thx9zh11	99	3	was	be	VBD
cord-328460-thx9zh11	99	4	composed	compose	VBN
cord-328460-thx9zh11	99	5	of	of	IN
cord-328460-thx9zh11	99	6	a	a	DT
cord-328460-thx9zh11	99	7	disposable	disposable	JJ
cord-328460-thx9zh11	99	8	polymethyl	polymethyl	JJ
cord-328460-thx9zh11	99	9	methacrylate	methacrylate	NN
cord-328460-thx9zh11	99	10	(	(	-LRB-
cord-328460-thx9zh11	99	11	PMMA	PMMA	NNP
cord-328460-thx9zh11	99	12	)	)	-RRB-
cord-328460-thx9zh11	99	13	micro	micro	JJ
cord-328460-thx9zh11	99	14	-	-	NN
cord-328460-thx9zh11	99	15	reactor	reactor	NN
cord-328460-thx9zh11	99	16	and	and	CC
cord-328460-thx9zh11	99	17	a	a	DT
cord-328460-thx9zh11	99	18	temperature	temperature	NN
cord-328460-thx9zh11	99	19	-	-	HYPH
cord-328460-thx9zh11	99	20	regulated	regulate	VBN
cord-328460-thx9zh11	99	21	optical	optical	JJ
cord-328460-thx9zh11	99	22	detection	detection	NN
cord-328460-thx9zh11	99	23	unit	unit	NN
cord-328460-thx9zh11	99	24	for	for	IN
cord-328460-thx9zh11	99	25	the	the	DT
cord-328460-thx9zh11	99	26	real	real	JJ
cord-328460-thx9zh11	99	27	-	-	HYPH
cord-328460-thx9zh11	99	28	time	time	NN
cord-328460-thx9zh11	99	29	monitoring	monitoring	NN
cord-328460-thx9zh11	99	30	of	of	IN
cord-328460-thx9zh11	99	31	the	the	DT
cord-328460-thx9zh11	99	32	turbidity	turbidity	NN
cord-328460-thx9zh11	99	33	changes	change	NNS
cord-328460-thx9zh11	99	34	due	due	JJ
cord-328460-thx9zh11	99	35	to	to	IN
cord-328460-thx9zh11	99	36	magnesium	magnesium	NN
cord-328460-thx9zh11	99	37	pyrophosphate	pyrophosphate	NN
cord-328460-thx9zh11	99	38	precipitation	precipitation	NN
cord-328460-thx9zh11	99	39	.	.	.
cord-328460-thx9zh11	100	1	An	an	DT
cord-328460-thx9zh11	100	2	important	important	JJ
cord-328460-thx9zh11	100	3	drawback	drawback	NN
cord-328460-thx9zh11	100	4	of	of	IN
cord-328460-thx9zh11	100	5	this	this	DT
cord-328460-thx9zh11	100	6	specific	specific	JJ
cord-328460-thx9zh11	100	7	detection	detection	NN
cord-328460-thx9zh11	100	8	scheme	scheme	NN
cord-328460-thx9zh11	100	9	is	be	VBZ
cord-328460-thx9zh11	100	10	represented	represent	VBN
cord-328460-thx9zh11	100	11	by	by	IN
cord-328460-thx9zh11	100	12	the	the	DT
cord-328460-thx9zh11	100	13	volume	volume	NN
cord-328460-thx9zh11	100	14	it	-PRON-	PRP
cord-328460-thx9zh11	100	15	requires	require	VBZ
cord-328460-thx9zh11	100	16	(	(	-LRB-
cord-328460-thx9zh11	100	17	LAMP	LAMP	NNP
cord-328460-thx9zh11	100	18	reaction	reaction	NN
cord-328460-thx9zh11	100	19	volume	volume	NN
cord-328460-thx9zh11	100	20	25	25	CD
cord-328460-thx9zh11	100	21	µL	µl	CD
cord-328460-thx9zh11	100	22	)	)	-RRB-
cord-328460-thx9zh11	101	1	[	[	-LRB-
cord-328460-thx9zh11	101	2	50	50	CD
cord-328460-thx9zh11	101	3	,	,	,
cord-328460-thx9zh11	101	4	51	51	CD
cord-328460-thx9zh11	101	5	]	]	-RRB-
cord-328460-thx9zh11	101	6	.	.	.
cord-328460-thx9zh11	102	1	It	-PRON-	PRP
cord-328460-thx9zh11	102	2	should	should	MD
cord-328460-thx9zh11	102	3	be	be	VB
cord-328460-thx9zh11	102	4	taken	take	VBN
cord-328460-thx9zh11	102	5	into	into	IN
cord-328460-thx9zh11	102	6	account	account	NN
cord-328460-thx9zh11	102	7	that	that	IN
cord-328460-thx9zh11	102	8	visual	visual	JJ
cord-328460-thx9zh11	102	9	detection	detection	NN
cord-328460-thx9zh11	102	10	from	from	IN
cord-328460-thx9zh11	102	11	microchannels	microchannel	NNS
cord-328460-thx9zh11	102	12	suffers	suffer	VBZ
cord-328460-thx9zh11	102	13	from	from	IN
cord-328460-thx9zh11	102	14	low	low	JJ
cord-328460-thx9zh11	102	15	sensitivity	sensitivity	NN
cord-328460-thx9zh11	102	16	as	as	IN
cord-328460-thx9zh11	102	17	a	a	DT
cord-328460-thx9zh11	102	18	consequence	consequence	NN
cord-328460-thx9zh11	102	19	of	of	IN
cord-328460-thx9zh11	102	20	the	the	DT
cord-328460-thx9zh11	102	21	short	short	JJ
cord-328460-thx9zh11	102	22	optical	optical	JJ
cord-328460-thx9zh11	102	23	length	length	NN
cord-328460-thx9zh11	102	24	.	.	.
cord-328460-thx9zh11	103	1	Zhang	Zhang	NNP
cord-328460-thx9zh11	103	2	et	et	NNP
cord-328460-thx9zh11	103	3	al	al	NNP
cord-328460-thx9zh11	103	4	.	.	.
cord-328460-thx9zh11	104	1	presented	present	VBD
cord-328460-thx9zh11	104	2	an	an	DT
cord-328460-thx9zh11	104	3	on	on	IN
cord-328460-thx9zh11	104	4	-	-	HYPH
cord-328460-thx9zh11	104	5	chip	chip	NN
cord-328460-thx9zh11	104	6	LAMP	lamp	NN
cord-328460-thx9zh11	104	7	carried	carry	VBD
cord-328460-thx9zh11	104	8	out	out	RP
cord-328460-thx9zh11	104	9	with	with	IN
cord-328460-thx9zh11	104	10	a	a	DT
cord-328460-thx9zh11	104	11	total	total	JJ
cord-328460-thx9zh11	104	12	reaction	reaction	NN
cord-328460-thx9zh11	104	13	volume	volume	NN
cord-328460-thx9zh11	104	14	of	of	IN
cord-328460-thx9zh11	104	15	10	10	CD
cord-328460-thx9zh11	104	16	μL	μl	NN
cord-328460-thx9zh11	104	17	for	for	IN
cord-328460-thx9zh11	104	18	visual	visual	JJ
cord-328460-thx9zh11	104	19	determination	determination	NN
cord-328460-thx9zh11	104	20	by	by	IN
cord-328460-thx9zh11	104	21	naked	naked	JJ
cord-328460-thx9zh11	104	22	eye	eye	NN
cord-328460-thx9zh11	104	23	with	with	IN
cord-328460-thx9zh11	105	1	SYBR	SYBR	NNP
cord-328460-thx9zh11	105	2	Green	Green	NNP
cord-328460-thx9zh11	105	3	I	I	NNP
cord-328460-thx9zh11	105	4	[	[	-LRB-
cord-328460-thx9zh11	105	5	52	52	CD
cord-328460-thx9zh11	105	6	]	]	-RRB-
cord-328460-thx9zh11	105	7	.	.	.
cord-328460-thx9zh11	106	1	The	the	DT
cord-328460-thx9zh11	106	2	reaction	reaction	NN
cord-328460-thx9zh11	106	3	volume	volume	NN
cord-328460-thx9zh11	106	4	has	have	VBZ
cord-328460-thx9zh11	106	5	been	be	VBN
cord-328460-thx9zh11	106	6	further	further	RB
cord-328460-thx9zh11	106	7	reduced	reduce	VBN
cord-328460-thx9zh11	106	8	to	to	IN
cord-328460-thx9zh11	106	9	5	5	CD
cord-328460-thx9zh11	106	10	μL	μL	NNS
cord-328460-thx9zh11	106	11	by	by	IN
cord-328460-thx9zh11	106	12	Fang	Fang	NNP
cord-328460-thx9zh11	106	13	et	et	FW
cord-328460-thx9zh11	106	14	al	al	NNP
cord-328460-thx9zh11	106	15	.	.	NNP
cord-328460-thx9zh11	106	16	,	,	,
cord-328460-thx9zh11	106	17	who	who	WP
cord-328460-thx9zh11	106	18	integrated	integrate	VBD
cord-328460-thx9zh11	106	19	the	the	DT
cord-328460-thx9zh11	106	20	LAMP	LAMP	NNP
cord-328460-thx9zh11	106	21	method	method	NN
cord-328460-thx9zh11	106	22	in	in	IN
cord-328460-thx9zh11	106	23	a	a	DT
cord-328460-thx9zh11	106	24	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	106	25	chip	chip	NN
cord-328460-thx9zh11	106	26	called	call	VBN
cord-328460-thx9zh11	106	27	microLAMP	microLAMP	NNP
cord-328460-thx9zh11	106	28	(	(	-LRB-
cord-328460-thx9zh11	106	29	μLAMP	μLAMP	NNP
cord-328460-thx9zh11	106	30	)	)	-RRB-
cord-328460-thx9zh11	106	31	to	to	TO
cord-328460-thx9zh11	106	32	quantitatively	quantitatively	RB
cord-328460-thx9zh11	106	33	detect	detect	VB
cord-328460-thx9zh11	106	34	target	target	NN
cord-328460-thx9zh11	106	35	nucleic	nucleic	JJ
cord-328460-thx9zh11	106	36	acids	acid	NNS
cord-328460-thx9zh11	106	37	[	[	-LRB-
cord-328460-thx9zh11	106	38	53	53	CD
cord-328460-thx9zh11	106	39	]	]	-RRB-
cord-328460-thx9zh11	106	40	.	.	.
cord-328460-thx9zh11	107	1	In	in	IN
cord-328460-thx9zh11	107	2	particular	particular	JJ
cord-328460-thx9zh11	107	3	,	,	,
cord-328460-thx9zh11	107	4	the	the	DT
cord-328460-thx9zh11	107	5	detection	detection	NN
cord-328460-thx9zh11	107	6	of	of	IN
cord-328460-thx9zh11	107	7	μLAMP	μLAMP	NNP
cord-328460-thx9zh11	107	8	was	be	VBD
cord-328460-thx9zh11	107	9	performed	perform	VBN
cord-328460-thx9zh11	107	10	in	in	IN
cord-328460-thx9zh11	107	11	an	an	DT
cord-328460-thx9zh11	107	12	eight	eight	CD
cord-328460-thx9zh11	107	13	-	-	HYPH
cord-328460-thx9zh11	107	14	channel	channel	NN
cord-328460-thx9zh11	107	15	chip	chip	NN
cord-328460-thx9zh11	107	16	for	for	IN
cord-328460-thx9zh11	107	17	readout	readout	NN
cord-328460-thx9zh11	107	18	either	either	CC
cord-328460-thx9zh11	107	19	by	by	IN
cord-328460-thx9zh11	107	20	the	the	DT
cord-328460-thx9zh11	107	21	naked	naked	JJ
cord-328460-thx9zh11	107	22	eye	eye	NN
cord-328460-thx9zh11	107	23	or	or	CC
cord-328460-thx9zh11	107	24	via	via	IN
cord-328460-thx9zh11	107	25	absorbance	absorbance	NN
cord-328460-thx9zh11	107	26	measured	measure	VBN
cord-328460-thx9zh11	107	27	by	by	IN
cord-328460-thx9zh11	107	28	an	an	DT
cord-328460-thx9zh11	107	29	optic	optic	JJ
cord-328460-thx9zh11	107	30	sensor	sensor	NN
cord-328460-thx9zh11	107	31	.	.	.
cord-328460-thx9zh11	108	1	Potential	potential	NN
cord-328460-thx9zh11	108	2	for	for	IN
cord-328460-thx9zh11	108	3	further	further	JJ
cord-328460-thx9zh11	108	4	miniaturization	miniaturization	NN
cord-328460-thx9zh11	108	5	has	have	VBZ
cord-328460-thx9zh11	108	6	been	be	VBN
cord-328460-thx9zh11	108	7	demonstrated	demonstrate	VBN
cord-328460-thx9zh11	108	8	by	by	IN
cord-328460-thx9zh11	108	9	the	the	DT
cord-328460-thx9zh11	108	10	successful	successful	JJ
cord-328460-thx9zh11	108	11	analysis	analysis	NN
cord-328460-thx9zh11	108	12	of	of	IN
cord-328460-thx9zh11	108	13	single	single	JJ
cord-328460-thx9zh11	108	14	DNA	dna	NN
cord-328460-thx9zh11	108	15	templates	template	NNS
cord-328460-thx9zh11	108	16	encapsulated	encapsulate	VBN
cord-328460-thx9zh11	108	17	in	in	IN
cord-328460-thx9zh11	108	18	a	a	DT
cord-328460-thx9zh11	108	19	polyacrylamide	polyacrylamide	NN
cord-328460-thx9zh11	109	1	(	(	-LRB-
cord-328460-thx9zh11	109	2	PAA	PAA	NNP
cord-328460-thx9zh11	109	3	)	)	-RRB-
cord-328460-thx9zh11	109	4	gel	gel	NN
cord-328460-thx9zh11	109	5	based	base	VBN
cord-328460-thx9zh11	109	6	microchamber	microchamber	NNP
cord-328460-thx9zh11	109	7	[	[	-LRB-
cord-328460-thx9zh11	109	8	54	54	CD
cord-328460-thx9zh11	109	9	]	]	-RRB-
cord-328460-thx9zh11	109	10	.	.	.
cord-328460-thx9zh11	110	1	A	a	DT
cord-328460-thx9zh11	110	2	microheater	microheater	NN
cord-328460-thx9zh11	110	3	,	,	,
cord-328460-thx9zh11	110	4	regulated	regulate	VBN
cord-328460-thx9zh11	110	5	by	by	IN
cord-328460-thx9zh11	110	6	an	an	DT
cord-328460-thx9zh11	110	7	automatic	automatic	JJ
cord-328460-thx9zh11	110	8	feedback	feedback	NN
cord-328460-thx9zh11	110	9	system	system	NN
cord-328460-thx9zh11	110	10	was	be	VBD
cord-328460-thx9zh11	110	11	used	use	VBN
cord-328460-thx9zh11	110	12	to	to	TO
cord-328460-thx9zh11	110	13	activate	activate	VB
cord-328460-thx9zh11	110	14	the	the	DT
cord-328460-thx9zh11	110	15	amplification	amplification	NN
cord-328460-thx9zh11	110	16	reaction	reaction	NN
cord-328460-thx9zh11	110	17	in	in	IN
cord-328460-thx9zh11	110	18	order	order	NN
cord-328460-thx9zh11	110	19	to	to	TO
cord-328460-thx9zh11	110	20	allow	allow	VB
cord-328460-thx9zh11	110	21	fluorescent	fluorescent	JJ
cord-328460-thx9zh11	110	22	imaging	imaging	NN
cord-328460-thx9zh11	110	23	of	of	IN
cord-328460-thx9zh11	110	24	LAMP	LAMP	NNP
cord-328460-thx9zh11	110	25	at	at	IN
cord-328460-thx9zh11	110	26	a	a	DT
cord-328460-thx9zh11	110	27	single	single	JJ
cord-328460-thx9zh11	110	28	molecule	molecule	NN
cord-328460-thx9zh11	110	29	level	level	NN
cord-328460-thx9zh11	110	30	.	.	.
cord-328460-thx9zh11	111	1	The	the	DT
cord-328460-thx9zh11	111	2	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	111	3	detection	detection	NN
cord-328460-thx9zh11	111	4	of	of	IN
cord-328460-thx9zh11	111	5	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	111	6	integrated	integrate	VBN
cord-328460-thx9zh11	111	7	LAMP	LAMP	NNP
cord-328460-thx9zh11	111	8	amplicons	amplicon	NNS
cord-328460-thx9zh11	111	9	has	have	VBZ
cord-328460-thx9zh11	111	10	been	be	VBN
cord-328460-thx9zh11	111	11	applied	apply	VBN
cord-328460-thx9zh11	111	12	for	for	IN
cord-328460-thx9zh11	111	13	the	the	DT
cord-328460-thx9zh11	111	14	detection	detection	NN
cord-328460-thx9zh11	111	15	and	and	CC
cord-328460-thx9zh11	111	16	the	the	DT
cord-328460-thx9zh11	111	17	quantification	quantification	NN
cord-328460-thx9zh11	111	18	of	of	IN
cord-328460-thx9zh11	111	19	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	111	20	coli	coli	NNS
cord-328460-thx9zh11	111	21	[	[	-LRB-
cord-328460-thx9zh11	111	22	55	55	CD
cord-328460-thx9zh11	111	23	]	]	-RRB-
cord-328460-thx9zh11	111	24	.	.	.
cord-328460-thx9zh11	112	1	Among	among	IN
cord-328460-thx9zh11	112	2	many	many	JJ
cord-328460-thx9zh11	112	3	sensing	sensing	NN
cord-328460-thx9zh11	112	4	techniques	technique	NNS
cord-328460-thx9zh11	112	5	developed	develop	VBN
cord-328460-thx9zh11	112	6	to	to	TO
cord-328460-thx9zh11	112	7	monitor	monitor	VB
cord-328460-thx9zh11	112	8	biorecognition	biorecognition	NN
cord-328460-thx9zh11	112	9	,	,	,
cord-328460-thx9zh11	112	10	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	112	11	methods	method	NNS
cord-328460-thx9zh11	112	12	provide	provide	VBP
cord-328460-thx9zh11	112	13	sensitivity	sensitivity	NN
cord-328460-thx9zh11	112	14	,	,	,
cord-328460-thx9zh11	112	15	selectivity	selectivity	NN
cord-328460-thx9zh11	112	16	,	,	,
cord-328460-thx9zh11	112	17	and	and	CC
cord-328460-thx9zh11	112	18	low	low	JJ
cord-328460-thx9zh11	112	19	cost	cost	NN
cord-328460-thx9zh11	112	20	detection	detection	NN
cord-328460-thx9zh11	112	21	of	of	IN
cord-328460-thx9zh11	112	22	the	the	DT
cord-328460-thx9zh11	112	23	amplified	amplify	VBN
cord-328460-thx9zh11	112	24	DNA	dna	NN
cord-328460-thx9zh11	112	25	sequences	sequence	NNS
cord-328460-thx9zh11	112	26	.	.	.
cord-328460-thx9zh11	113	1	If	if	IN
cord-328460-thx9zh11	113	2	compared	compare	VBN
cord-328460-thx9zh11	113	3	with	with	IN
cord-328460-thx9zh11	113	4	optical	optical	JJ
cord-328460-thx9zh11	113	5	detection	detection	NN
cord-328460-thx9zh11	113	6	,	,	,
cord-328460-thx9zh11	113	7	the	the	DT
cord-328460-thx9zh11	113	8	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	113	9	detection	detection	NN
cord-328460-thx9zh11	113	10	shows	show	VBZ
cord-328460-thx9zh11	113	11	significant	significant	JJ
cord-328460-thx9zh11	113	12	advantages	advantage	NNS
cord-328460-thx9zh11	113	13	such	such	JJ
cord-328460-thx9zh11	113	14	as	as	IN
cord-328460-thx9zh11	113	15	independence	independence	NN
cord-328460-thx9zh11	113	16	from	from	IN
cord-328460-thx9zh11	113	17	sample	sample	NN
cord-328460-thx9zh11	113	18	turbidity	turbidity	NN
cord-328460-thx9zh11	113	19	and	and	CC
cord-328460-thx9zh11	113	20	extremely	extremely	RB
cord-328460-thx9zh11	113	21	low	low	JJ
cord-328460-thx9zh11	113	22	-	-	HYPH
cord-328460-thx9zh11	113	23	cost	cost	NN
cord-328460-thx9zh11	113	24	/	/	SYM
cord-328460-thx9zh11	113	25	low	low	JJ
cord-328460-thx9zh11	113	26	-	-	HYPH
cord-328460-thx9zh11	113	27	power	power	NN
cord-328460-thx9zh11	113	28	requirements	requirement	NNS
cord-328460-thx9zh11	113	29	,	,	,
cord-328460-thx9zh11	113	30	in	in	IN
cord-328460-thx9zh11	113	31	addition	addition	NN
cord-328460-thx9zh11	113	32	to	to	IN
cord-328460-thx9zh11	113	33	its	-PRON-	PRP$
cord-328460-thx9zh11	113	34	compatibility	compatibility	NN
cord-328460-thx9zh11	113	35	with	with	IN
cord-328460-thx9zh11	113	36	miniaturization	miniaturization	NN
cord-328460-thx9zh11	113	37	processes	process	NNS
cord-328460-thx9zh11	113	38	.	.	.
cord-328460-thx9zh11	114	1	Unlike	unlike	IN
cord-328460-thx9zh11	114	2	other	other	JJ
cord-328460-thx9zh11	114	3	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	114	4	techniques	technique	NNS
cord-328460-thx9zh11	114	5	[	[	-LRB-
cord-328460-thx9zh11	114	6	56	56	CD
cord-328460-thx9zh11	114	7	]	]	-RRB-
cord-328460-thx9zh11	114	8	,	,	,
cord-328460-thx9zh11	114	9	based	base	VBN
cord-328460-thx9zh11	114	10	on	on	IN
cord-328460-thx9zh11	114	11	the	the	DT
cord-328460-thx9zh11	114	12	immobilization	immobilization	NN
cord-328460-thx9zh11	114	13	of	of	IN
cord-328460-thx9zh11	114	14	an	an	DT
cord-328460-thx9zh11	114	15	oligonucleotide	oligonucleotide	JJ
cord-328460-thx9zh11	114	16	probe	probe	NN
cord-328460-thx9zh11	114	17	onto	onto	IN
cord-328460-thx9zh11	114	18	an	an	DT
cord-328460-thx9zh11	114	19	electrode	electrode	NN
cord-328460-thx9zh11	114	20	,	,	,
cord-328460-thx9zh11	114	21	hybridization	hybridization	NN
cord-328460-thx9zh11	114	22	of	of	IN
cord-328460-thx9zh11	114	23	a	a	DT
cord-328460-thx9zh11	114	24	complementary	complementary	JJ
cord-328460-thx9zh11	114	25	target	target	NN
cord-328460-thx9zh11	114	26	sequence	sequence	NN
cord-328460-thx9zh11	114	27	,	,	,
cord-328460-thx9zh11	114	28	and	and	CC
cord-328460-thx9zh11	114	29	transduction	transduction	NN
cord-328460-thx9zh11	114	30	of	of	IN
cord-328460-thx9zh11	114	31	the	the	DT
cord-328460-thx9zh11	114	32	hybridization	hybridization	NN
cord-328460-thx9zh11	114	33	event	event	NN
cord-328460-thx9zh11	114	34	[	[	-LRB-
cord-328460-thx9zh11	114	35	57	57	CD
cord-328460-thx9zh11	114	36	]	]	-RRB-
cord-328460-thx9zh11	114	37	,	,	,
cord-328460-thx9zh11	114	38	the	the	DT
cord-328460-thx9zh11	114	39	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	114	40	detection	detection	NN
cord-328460-thx9zh11	114	41	of	of	IN
cord-328460-thx9zh11	114	42	LAMP	LAMP	NNP
cord-328460-thx9zh11	114	43	amplicons	amplicon	VBZ
cord-328460-thx9zh11	114	44	[	[	-LRB-
cord-328460-thx9zh11	114	45	55	55	CD
cord-328460-thx9zh11	114	46	]	]	-RRB-
cord-328460-thx9zh11	114	47	does	do	VBZ
cord-328460-thx9zh11	114	48	not	not	RB
cord-328460-thx9zh11	114	49	require	require	VB
cord-328460-thx9zh11	114	50	probe	probe	NN
cord-328460-thx9zh11	114	51	immobilization	immobilization	NN
cord-328460-thx9zh11	114	52	and	and	CC
cord-328460-thx9zh11	114	53	bacteria	bacteria	NNS
cord-328460-thx9zh11	114	54	detection	detection	NN
cord-328460-thx9zh11	114	55	can	can	MD
cord-328460-thx9zh11	114	56	be	be	VB
cord-328460-thx9zh11	114	57	accomplished	accomplish	VBN
cord-328460-thx9zh11	114	58	in	in	IN
cord-328460-thx9zh11	114	59	a	a	DT
cord-328460-thx9zh11	114	60	single	single	JJ
cord-328460-thx9zh11	114	61	chamber	chamber	NN
cord-328460-thx9zh11	114	62	without	without	IN
cord-328460-thx9zh11	114	63	DNA	dna	NN
cord-328460-thx9zh11	114	64	extraction	extraction	NN
cord-328460-thx9zh11	114	65	and	and	CC
cord-328460-thx9zh11	114	66	purification	purification	NN
cord-328460-thx9zh11	114	67	steps	step	NNS
cord-328460-thx9zh11	114	68	,	,	,
cord-328460-thx9zh11	114	69	since	since	IN
cord-328460-thx9zh11	114	70	the	the	DT
cord-328460-thx9zh11	114	71	used	use	VBN
cord-328460-thx9zh11	114	72	isothermal	isothermal	JJ
cord-328460-thx9zh11	114	73	temperature	temperature	NN
cord-328460-thx9zh11	114	74	(	(	-LRB-
cord-328460-thx9zh11	114	75	66	66	CD
cord-328460-thx9zh11	114	76	°	°	NN
cord-328460-thx9zh11	114	77	C	c	NN
cord-328460-thx9zh11	114	78	)	)	-RRB-
cord-328460-thx9zh11	114	79	provides	provide	VBZ
cord-328460-thx9zh11	114	80	enough	enough	JJ
cord-328460-thx9zh11	114	81	thermal	thermal	JJ
cord-328460-thx9zh11	114	82	shock	shock	NN
cord-328460-thx9zh11	114	83	to	to	TO
cord-328460-thx9zh11	114	84	lyse	lyse	VB
cord-328460-thx9zh11	114	85	the	the	DT
cord-328460-thx9zh11	114	86	E.	E.	NNP
cord-328460-thx9zh11	114	87	coli	coli	NNS
cord-328460-thx9zh11	114	88	bacterial	bacterial	JJ
cord-328460-thx9zh11	114	89	target	target	NN
cord-328460-thx9zh11	114	90	without	without	IN
cord-328460-thx9zh11	114	91	any	any	DT
cord-328460-thx9zh11	114	92	pre	pre	JJ
cord-328460-thx9zh11	114	93	-	-	NN
cord-328460-thx9zh11	114	94	treatment	treatment	NN
cord-328460-thx9zh11	114	95	.	.	.
cord-328460-thx9zh11	115	1	Progresses	progress	NNS
cord-328460-thx9zh11	115	2	in	in	IN
cord-328460-thx9zh11	115	3	understanding	understand	VBG
cord-328460-thx9zh11	115	4	properties	property	NNS
cord-328460-thx9zh11	115	5	of	of	IN
cord-328460-thx9zh11	115	6	helicases	helicase	NNS
cord-328460-thx9zh11	115	7	,	,	,
cord-328460-thx9zh11	115	8	discovered	discover	VBN
cord-328460-thx9zh11	115	9	in	in	IN
cord-328460-thx9zh11	115	10	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	115	11	coli	coli	NNS
cord-328460-thx9zh11	115	12	in	in	IN
cord-328460-thx9zh11	115	13	1976	1976	CD
cord-328460-thx9zh11	115	14	[	[	-LRB-
cord-328460-thx9zh11	115	15	58	58	CD
cord-328460-thx9zh11	115	16	,	,	,
cord-328460-thx9zh11	115	17	59	59	CD
cord-328460-thx9zh11	115	18	]	]	-RRB-
cord-328460-thx9zh11	115	19	,	,	,
cord-328460-thx9zh11	115	20	have	have	VBP
cord-328460-thx9zh11	115	21	enabled	enable	VBN
cord-328460-thx9zh11	115	22	researchers	researcher	NNS
cord-328460-thx9zh11	115	23	to	to	TO
cord-328460-thx9zh11	115	24	combine	combine	VB
cord-328460-thx9zh11	115	25	helicases	helicase	NNS
cord-328460-thx9zh11	115	26	with	with	IN
cord-328460-thx9zh11	115	27	polymerases	polymerase	NNS
cord-328460-thx9zh11	115	28	and	and	CC
cord-328460-thx9zh11	115	29	other	other	JJ
cord-328460-thx9zh11	115	30	accessory	accessory	NN
cord-328460-thx9zh11	115	31	proteins	protein	NNS
cord-328460-thx9zh11	115	32	for	for	IN
cord-328460-thx9zh11	115	33	applications	application	NNS
cord-328460-thx9zh11	115	34	in	in	IN
cord-328460-thx9zh11	115	35	nucleic	nucleic	JJ
cord-328460-thx9zh11	115	36	acid	acid	NN
cord-328460-thx9zh11	115	37	amplification	amplification	NN
cord-328460-thx9zh11	115	38	.	.	.
cord-328460-thx9zh11	116	1	Helicase	helicase	JJ
cord-328460-thx9zh11	116	2	-	-	HYPH
cord-328460-thx9zh11	116	3	dependent	dependent	JJ
cord-328460-thx9zh11	116	4	amplification	amplification	NN
cord-328460-thx9zh11	116	5	(	(	-LRB-
cord-328460-thx9zh11	116	6	HDA	HDA	NNP
cord-328460-thx9zh11	116	7	)	)	-RRB-
cord-328460-thx9zh11	116	8	exploits	exploit	VBZ
cord-328460-thx9zh11	116	9	the	the	DT
cord-328460-thx9zh11	116	10	activity	activity	NN
cord-328460-thx9zh11	116	11	of	of	IN
cord-328460-thx9zh11	116	12	a	a	DT
cord-328460-thx9zh11	116	13	DNA	dna	NN
cord-328460-thx9zh11	116	14	helicase	helicase	NN
cord-328460-thx9zh11	116	15	to	to	TO
cord-328460-thx9zh11	116	16	separate	separate	VB
cord-328460-thx9zh11	116	17	complementary	complementary	JJ
cord-328460-thx9zh11	116	18	strands	strand	NNS
cord-328460-thx9zh11	116	19	of	of	IN
cord-328460-thx9zh11	116	20	double	double	JJ
cord-328460-thx9zh11	116	21	strand	strand	NN
cord-328460-thx9zh11	116	22	(	(	-LRB-
cord-328460-thx9zh11	116	23	ds	ds	NNP
cord-328460-thx9zh11	116	24	)	)	-RRB-
cord-328460-thx9zh11	116	25	DNAs	DNAs	NNP
cord-328460-thx9zh11	116	26	thus	thus	RB
cord-328460-thx9zh11	116	27	avoiding	avoid	VBG
cord-328460-thx9zh11	116	28	the	the	DT
cord-328460-thx9zh11	116	29	temperature	temperature	NN
cord-328460-thx9zh11	116	30	cycling	cycling	NN
cord-328460-thx9zh11	116	31	to	to	TO
cord-328460-thx9zh11	116	32	produce	produce	VB
cord-328460-thx9zh11	116	33	single	single	JJ
cord-328460-thx9zh11	116	34	-	-	HYPH
cord-328460-thx9zh11	116	35	stranded	strand	VBN
cord-328460-thx9zh11	116	36	templates	template	NNS
cord-328460-thx9zh11	116	37	for	for	IN
cord-328460-thx9zh11	116	38	primer	primer	NN
cord-328460-thx9zh11	116	39	hybridization	hybridization	NN
cord-328460-thx9zh11	116	40	and	and	CC
cord-328460-thx9zh11	116	41	subsequent	subsequent	JJ
cord-328460-thx9zh11	116	42	primer	primer	NN
cord-328460-thx9zh11	116	43	extension	extension	NN
cord-328460-thx9zh11	116	44	by	by	IN
cord-328460-thx9zh11	116	45	a	a	DT
cord-328460-thx9zh11	116	46	DNA	dna	NN
cord-328460-thx9zh11	116	47	polymerase	polymerase	NN
cord-328460-thx9zh11	116	48	[	[	-LRB-
cord-328460-thx9zh11	116	49	60	60	CD
cord-328460-thx9zh11	116	50	]	]	-RRB-
cord-328460-thx9zh11	116	51	.	.	.
cord-328460-thx9zh11	117	1	It	-PRON-	PRP
cord-328460-thx9zh11	117	2	mimics	mimic	VBZ
cord-328460-thx9zh11	117	3	the	the	DT
cord-328460-thx9zh11	117	4	denaturation	denaturation	NN
cord-328460-thx9zh11	117	5	mechanism	mechanism	NN
cord-328460-thx9zh11	117	6	in	in	IN
cord-328460-thx9zh11	117	7	living	live	VBG
cord-328460-thx9zh11	117	8	organisms	organism	NNS
cord-328460-thx9zh11	117	9	where	where	WRB
cord-328460-thx9zh11	117	10	DNA	DNA	NNP
cord-328460-thx9zh11	117	11	is	be	VBZ
cord-328460-thx9zh11	117	12	replicated	replicate	VBN
cord-328460-thx9zh11	117	13	by	by	IN
cord-328460-thx9zh11	117	14	DNA	dna	NN
cord-328460-thx9zh11	117	15	polymerases	polymerase	NNS
cord-328460-thx9zh11	117	16	with	with	IN
cord-328460-thx9zh11	117	17	the	the	DT
cord-328460-thx9zh11	117	18	aid	aid	NN
cord-328460-thx9zh11	117	19	of	of	IN
cord-328460-thx9zh11	117	20	DNA	dna	NN
cord-328460-thx9zh11	117	21	helicase	helicase	NN
cord-328460-thx9zh11	117	22	to	to	TO
cord-328460-thx9zh11	117	23	separate	separate	VB
cord-328460-thx9zh11	117	24	complementary	complementary	JJ
cord-328460-thx9zh11	117	25	DNA	dna	NN
cord-328460-thx9zh11	117	26	strands	strand	NNS
cord-328460-thx9zh11	117	27	.	.	.
cord-328460-thx9zh11	118	1	A	a	DT
cord-328460-thx9zh11	118	2	scheme	scheme	NN
cord-328460-thx9zh11	118	3	of	of	IN
cord-328460-thx9zh11	118	4	HDA	hda	NN
cord-328460-thx9zh11	118	5	mechanism	mechanism	NN
cord-328460-thx9zh11	118	6	is	be	VBZ
cord-328460-thx9zh11	118	7	shown	show	VBN
cord-328460-thx9zh11	118	8	in	in	IN
cord-328460-thx9zh11	118	9	Figure	figure	NN
cord-328460-thx9zh11	118	10	2	2	CD
cord-328460-thx9zh11	118	11	.	.	.
cord-328460-thx9zh11	119	1	It	-PRON-	PRP
cord-328460-thx9zh11	119	2	mimics	mimic	VBZ
cord-328460-thx9zh11	119	3	the	the	DT
cord-328460-thx9zh11	119	4	replication	replication	NN
cord-328460-thx9zh11	119	5	fork	fork	NN
cord-328460-thx9zh11	119	6	and	and	CC
cord-328460-thx9zh11	119	7	enables	enable	VBZ
cord-328460-thx9zh11	119	8	DNA	dna	NN
cord-328460-thx9zh11	119	9	synthesis	synthesis	NN
cord-328460-thx9zh11	119	10	to	to	TO
cord-328460-thx9zh11	119	11	occur	occur	VB
cord-328460-thx9zh11	119	12	by	by	IN
cord-328460-thx9zh11	119	13	using	use	VBG
cord-328460-thx9zh11	119	14	chemical	chemical	JJ
cord-328460-thx9zh11	119	15	energy	energy	NN
cord-328460-thx9zh11	119	16	[	[	-LRB-
cord-328460-thx9zh11	119	17	61	61	CD
cord-328460-thx9zh11	119	18	]	]	-RRB-
cord-328460-thx9zh11	119	19	.	.	.
cord-328460-thx9zh11	120	1	The	the	DT
cord-328460-thx9zh11	120	2	helicase	helicase	NN
cord-328460-thx9zh11	120	3	enzyme	enzyme	NN
cord-328460-thx9zh11	120	4	in	in	IN
cord-328460-thx9zh11	120	5	the	the	DT
cord-328460-thx9zh11	120	6	presence	presence	NN
cord-328460-thx9zh11	120	7	of	of	IN
cord-328460-thx9zh11	120	8	ATP	ATP	NNP
cord-328460-thx9zh11	120	9	loads	load	NNS
cord-328460-thx9zh11	120	10	on	on	RP
cord-328460-thx9zh11	120	11	to	to	IN
cord-328460-thx9zh11	120	12	the	the	DT
cord-328460-thx9zh11	120	13	dsDNA	dsdna	JJ
cord-328460-thx9zh11	120	14	template	template	NN
cord-328460-thx9zh11	120	15	and	and	CC
cord-328460-thx9zh11	120	16	traverses	traverse	VBZ
cord-328460-thx9zh11	120	17	along	along	IN
cord-328460-thx9zh11	120	18	the	the	DT
cord-328460-thx9zh11	120	19	target	target	NN
cord-328460-thx9zh11	120	20	DNA	dna	NN
cord-328460-thx9zh11	120	21	,	,	,
cord-328460-thx9zh11	120	22	disrupting	disrupt	VBG
cord-328460-thx9zh11	120	23	the	the	DT
cord-328460-thx9zh11	120	24	hydrogen	hydrogen	NN
cord-328460-thx9zh11	120	25	bonds	bond	NNS
cord-328460-thx9zh11	120	26	linking	link	VBG
cord-328460-thx9zh11	120	27	the	the	DT
cord-328460-thx9zh11	120	28	two	two	CD
cord-328460-thx9zh11	120	29	strands	strand	NNS
cord-328460-thx9zh11	120	30	.	.	.
cord-328460-thx9zh11	121	1	The	the	DT
cord-328460-thx9zh11	121	2	formed	form	VBN
cord-328460-thx9zh11	121	3	single	single	JJ
cord-328460-thx9zh11	121	4	strand	strand	NN
cord-328460-thx9zh11	121	5	(	(	-LRB-
cord-328460-thx9zh11	121	6	ss	ss	NNP
cord-328460-thx9zh11	121	7	)	)	-RRB-
cord-328460-thx9zh11	121	8	DNAs	dna	NNS
cord-328460-thx9zh11	121	9	are	be	VBP
cord-328460-thx9zh11	121	10	then	then	RB
cord-328460-thx9zh11	121	11	coated	coat	VBN
cord-328460-thx9zh11	121	12	by	by	IN
cord-328460-thx9zh11	121	13	single	single	RB
cord-328460-thx9zh11	121	14	-	-	HYPH
cord-328460-thx9zh11	121	15	stranded	strand	VBN
cord-328460-thx9zh11	121	16	binding	binding	NN
cord-328460-thx9zh11	121	17	proteins	protein	NNS
cord-328460-thx9zh11	121	18	(	(	-LRB-
cord-328460-thx9zh11	121	19	SSBs	ssb	NNS
cord-328460-thx9zh11	121	20	;	;	:
cord-328460-thx9zh11	121	21	Figure	figure	NN
cord-328460-thx9zh11	121	22	2	2	CD
cord-328460-thx9zh11	121	23	,	,	,
cord-328460-thx9zh11	121	24	step	step	NN
cord-328460-thx9zh11	121	25	1	1	CD
cord-328460-thx9zh11	121	26	)	)	-RRB-
cord-328460-thx9zh11	121	27	.	.	.
cord-328460-thx9zh11	122	1	Two	two	CD
cord-328460-thx9zh11	122	2	sequence	sequence	NN
cord-328460-thx9zh11	122	3	-	-	HYPH
cord-328460-thx9zh11	122	4	specific	specific	JJ
cord-328460-thx9zh11	122	5	primers	primer	NNS
cord-328460-thx9zh11	122	6	hybridize	hybridize	VBP
cord-328460-thx9zh11	122	7	to	to	IN
cord-328460-thx9zh11	122	8	the	the	DT
cord-328460-thx9zh11	122	9	3'-end	3'-end	CD
cord-328460-thx9zh11	122	10	of	of	IN
cord-328460-thx9zh11	122	11	each	each	DT
cord-328460-thx9zh11	122	12	ssDNA	ssdna	JJ
cord-328460-thx9zh11	122	13	template	template	NN
cord-328460-thx9zh11	122	14	(	(	-LRB-
cord-328460-thx9zh11	122	15	Figure	figure	NN
cord-328460-thx9zh11	122	16	2	2	CD
cord-328460-thx9zh11	122	17	,	,	,
cord-328460-thx9zh11	122	18	step	step	NN
cord-328460-thx9zh11	122	19	2	2	CD
cord-328460-thx9zh11	122	20	)	)	-RRB-
cord-328460-thx9zh11	122	21	.	.	.
cord-328460-thx9zh11	123	1	DNA	DNA	NNP
cord-328460-thx9zh11	123	2	polymerases	polymerase	NNS
cord-328460-thx9zh11	123	3	extend	extend	VBP
cord-328460-thx9zh11	123	4	primers	primer	NNS
cord-328460-thx9zh11	123	5	annealed	anneal	VBN
cord-328460-thx9zh11	123	6	to	to	IN
cord-328460-thx9zh11	123	7	the	the	DT
cord-328460-thx9zh11	123	8	target	target	NN
cord-328460-thx9zh11	123	9	by	by	IN
cord-328460-thx9zh11	123	10	producing	produce	VBG
cord-328460-thx9zh11	123	11	dsDNA	dsdna	RB
cord-328460-thx9zh11	124	1	(	(	-LRB-
cord-328460-thx9zh11	124	2	Figure	figure	NN
cord-328460-thx9zh11	124	3	2	2	CD
cord-328460-thx9zh11	124	4	,	,	,
cord-328460-thx9zh11	124	5	step	step	NN
cord-328460-thx9zh11	124	6	3	3	CD
cord-328460-thx9zh11	124	7	)	)	-RRB-
cord-328460-thx9zh11	124	8	.	.	.
cord-328460-thx9zh11	125	1	The	the	DT
cord-328460-thx9zh11	125	2	two	two	CD
cord-328460-thx9zh11	125	3	newly	newly	RB
cord-328460-thx9zh11	125	4	synthesized	synthesize	VBN
cord-328460-thx9zh11	125	5	dsDNA	dsDNA	NNP
cord-328460-thx9zh11	125	6	products	product	NNS
cord-328460-thx9zh11	125	7	act	act	VBP
cord-328460-thx9zh11	125	8	then	then	RB
cord-328460-thx9zh11	125	9	as	as	IN
cord-328460-thx9zh11	125	10	substrates	substrate	NNS
cord-328460-thx9zh11	125	11	for	for	IN
cord-328460-thx9zh11	125	12	DNA	dna	NN
cord-328460-thx9zh11	125	13	helicases	helicase	NNS
cord-328460-thx9zh11	125	14	in	in	IN
cord-328460-thx9zh11	125	15	the	the	DT
cord-328460-thx9zh11	125	16	next	next	JJ
cord-328460-thx9zh11	125	17	round	round	NN
cord-328460-thx9zh11	125	18	of	of	IN
cord-328460-thx9zh11	125	19	the	the	DT
cord-328460-thx9zh11	125	20	reaction	reaction	NN
cord-328460-thx9zh11	125	21	(	(	-LRB-
cord-328460-thx9zh11	125	22	Figure	figure	NN
cord-328460-thx9zh11	125	23	2	2	CD
cord-328460-thx9zh11	125	24	,	,	,
cord-328460-thx9zh11	125	25	step	step	NN
cord-328460-thx9zh11	125	26	4	4	CD
cord-328460-thx9zh11	125	27	)	)	-RRB-
cord-328460-thx9zh11	125	28	,	,	,
cord-328460-thx9zh11	125	29	resulting	result	VBG
cord-328460-thx9zh11	125	30	in	in	IN
cord-328460-thx9zh11	125	31	an	an	DT
cord-328460-thx9zh11	125	32	exponential	exponential	JJ
cord-328460-thx9zh11	125	33	amplification	amplification	NN
cord-328460-thx9zh11	125	34	of	of	IN
cord-328460-thx9zh11	125	35	the	the	DT
cord-328460-thx9zh11	125	36	selected	select	VBN
cord-328460-thx9zh11	125	37	target	target	NN
cord-328460-thx9zh11	125	38	sequence	sequence	NN
cord-328460-thx9zh11	125	39	.	.	.
cord-328460-thx9zh11	126	1	Helicases	Helicases	NNP
cord-328460-thx9zh11	126	2	are	be	VBP
cord-328460-thx9zh11	126	3	motor	motor	NN
cord-328460-thx9zh11	126	4	proteins	protein	NNS
cord-328460-thx9zh11	126	5	that	that	WDT
cord-328460-thx9zh11	126	6	bind	bind	VBP
cord-328460-thx9zh11	126	7	nucleic	nucleic	JJ
cord-328460-thx9zh11	126	8	acids	acid	NNS
cord-328460-thx9zh11	126	9	and	and	CC
cord-328460-thx9zh11	126	10	move	move	VBP
cord-328460-thx9zh11	126	11	directionally	directionally	RB
cord-328460-thx9zh11	126	12	along	along	IN
cord-328460-thx9zh11	126	13	them	-PRON-	PRP
cord-328460-thx9zh11	126	14	.	.	.
cord-328460-thx9zh11	127	1	They	-PRON-	PRP
cord-328460-thx9zh11	127	2	separate	separate	VBP
cord-328460-thx9zh11	127	3	dsDNA	dsdna	RB
cord-328460-thx9zh11	127	4	by	by	IN
cord-328460-thx9zh11	127	5	using	use	VBG
cord-328460-thx9zh11	127	6	the	the	DT
cord-328460-thx9zh11	127	7	energy	energy	NN
cord-328460-thx9zh11	127	8	generated	generate	VBN
cord-328460-thx9zh11	127	9	by	by	IN
cord-328460-thx9zh11	127	10	the	the	DT
cord-328460-thx9zh11	127	11	nucleoside	nucleoside	NN
cord-328460-thx9zh11	128	1	5'-triphosphate	5'-triphosphate	JJ
cord-328460-thx9zh11	128	2	(	(	-LRB-
cord-328460-thx9zh11	128	3	NTP	NTP	NNP
cord-328460-thx9zh11	128	4	)	)	-RRB-
cord-328460-thx9zh11	128	5	hydrolysis	hydrolysis	NN
cord-328460-thx9zh11	128	6	.	.	.
cord-328460-thx9zh11	129	1	Helicases	Helicases	NNP
cord-328460-thx9zh11	129	2	can	can	MD
cord-328460-thx9zh11	129	3	be	be	VB
cord-328460-thx9zh11	129	4	classified	classify	VBN
cord-328460-thx9zh11	129	5	according	accord	VBG
cord-328460-thx9zh11	129	6	to	to	IN
cord-328460-thx9zh11	129	7	their	-PRON-	PRP$
cord-328460-thx9zh11	129	8	directionality	directionality	NN
cord-328460-thx9zh11	129	9	(	(	-LRB-
cord-328460-thx9zh11	129	10	e.g.	e.g.	RB
cord-328460-thx9zh11	129	11	,	,	,
cord-328460-thx9zh11	129	12	5'-3	5'-3	CD
cord-328460-thx9zh11	129	13	'	'	''
cord-328460-thx9zh11	129	14	or	or	CC
cord-328460-thx9zh11	129	15	3'-5	3'-5	NNP
cord-328460-thx9zh11	129	16	'	'	''
cord-328460-thx9zh11	129	17	)	)	-RRB-
cord-328460-thx9zh11	129	18	,	,	,
cord-328460-thx9zh11	129	19	their	-PRON-	PRP$
cord-328460-thx9zh11	129	20	source	source	NN
cord-328460-thx9zh11	129	21	(	(	-LRB-
cord-328460-thx9zh11	129	22	e.g.	e.g.	RB
cord-328460-thx9zh11	129	23	,	,	,
cord-328460-thx9zh11	129	24	prokaryotic	prokaryotic	JJ
cord-328460-thx9zh11	129	25	or	or	CC
cord-328460-thx9zh11	129	26	eukaryotic	eukaryotic	JJ
cord-328460-thx9zh11	129	27	helicases	helicase	NNS
cord-328460-thx9zh11	129	28	)	)	-RRB-
cord-328460-thx9zh11	129	29	,	,	,
cord-328460-thx9zh11	129	30	their	-PRON-	PRP$
cord-328460-thx9zh11	129	31	oligomeric	oligomeric	JJ
cord-328460-thx9zh11	129	32	or	or	CC
cord-328460-thx9zh11	129	33	monomeric	monomeric	JJ
cord-328460-thx9zh11	129	34	nature	nature	NN
cord-328460-thx9zh11	129	35	and	and	CC
cord-328460-thx9zh11	129	36	their	-PRON-	PRP$
cord-328460-thx9zh11	129	37	substrate	substrate	NN
cord-328460-thx9zh11	129	38	specificity	specificity	NN
cord-328460-thx9zh11	129	39	(	(	-LRB-
cord-328460-thx9zh11	129	40	e.g.	e.g.	RB
cord-328460-thx9zh11	129	41	,	,	,
cord-328460-thx9zh11	129	42	RNA	RNA	NNP
cord-328460-thx9zh11	129	43	vs.	vs.	IN
cord-328460-thx9zh11	129	44	DNA	DNA	NNP
cord-328460-thx9zh11	129	45	)	)	-RRB-
cord-328460-thx9zh11	129	46	[	[	-LRB-
cord-328460-thx9zh11	129	47	62	62	CD
cord-328460-thx9zh11	129	48	]	]	-RRB-
cord-328460-thx9zh11	129	49	[	[	-LRB-
cord-328460-thx9zh11	129	50	63	63	CD
cord-328460-thx9zh11	129	51	]	]	-RRB-
cord-328460-thx9zh11	129	52	[	[	-LRB-
cord-328460-thx9zh11	129	53	64	64	CD
cord-328460-thx9zh11	129	54	]	]	-RRB-
cord-328460-thx9zh11	129	55	.	.	.
cord-328460-thx9zh11	130	1	SF1	SF1	NNP
cord-328460-thx9zh11	130	2	and	and	CC
cord-328460-thx9zh11	130	3	SF2	SF2	NNP
cord-328460-thx9zh11	130	4	helicase	helicase	NN
cord-328460-thx9zh11	130	5	structures	structure	NNS
cord-328460-thx9zh11	130	6	have	have	VBP
cord-328460-thx9zh11	130	7	shown	show	VBN
cord-328460-thx9zh11	130	8	that	that	IN
cord-328460-thx9zh11	130	9	the	the	DT
cord-328460-thx9zh11	130	10	DNA	dna	NN
cord-328460-thx9zh11	130	11	helicase	helicase	NN
cord-328460-thx9zh11	130	12	motifs	motif	NNS
cord-328460-thx9zh11	130	13	are	be	VBP
cord-328460-thx9zh11	130	14	clustered	cluster	VBN
cord-328460-thx9zh11	130	15	together	together	RB
cord-328460-thx9zh11	130	16	in	in	IN
cord-328460-thx9zh11	130	17	the	the	DT
cord-328460-thx9zh11	130	18	tertiary	tertiary	JJ
cord-328460-thx9zh11	130	19	structure	structure	NN
cord-328460-thx9zh11	130	20	,	,	,
cord-328460-thx9zh11	130	21	forming	form	VBG
cord-328460-thx9zh11	130	22	a	a	DT
cord-328460-thx9zh11	130	23	nucleic	nucleic	JJ
cord-328460-thx9zh11	130	24	acid	acid	NN
cord-328460-thx9zh11	130	25	-	-	HYPH
cord-328460-thx9zh11	130	26	binding	bind	VBG
cord-328460-thx9zh11	130	27	site	site	NN
cord-328460-thx9zh11	130	28	and	and	CC
cord-328460-thx9zh11	130	29	an	an	DT
cord-328460-thx9zh11	130	30	NTP	NTP	NNP
cord-328460-thx9zh11	130	31	-	-	HYPH
cord-328460-thx9zh11	130	32	binding	bind	VBG
cord-328460-thx9zh11	130	33	pocket	pocket	NN
cord-328460-thx9zh11	130	34	[	[	-LRB-
cord-328460-thx9zh11	130	35	62	62	CD
cord-328460-thx9zh11	130	36	]	]	-RRB-
cord-328460-thx9zh11	130	37	.	.	.
cord-328460-thx9zh11	131	1	Conserved	conserved	JJ
cord-328460-thx9zh11	131	2	motifs	motif	NNS
cord-328460-thx9zh11	131	3	can	can	MD
cord-328460-thx9zh11	131	4	be	be	VB
cord-328460-thx9zh11	131	5	envisioned	envision	VBN
cord-328460-thx9zh11	131	6	as	as	IN
cord-328460-thx9zh11	131	7	an	an	DT
cord-328460-thx9zh11	131	8	engine	engine	NN
cord-328460-thx9zh11	131	9	that	that	WDT
cord-328460-thx9zh11	131	10	powers	power	VBZ
cord-328460-thx9zh11	131	11	the	the	DT
cord-328460-thx9zh11	131	12	unwinding	unwinding	NN
cord-328460-thx9zh11	131	13	of	of	IN
cord-328460-thx9zh11	131	14	duplex	duplex	JJ
cord-328460-thx9zh11	131	15	nucleic	nucleic	JJ
cord-328460-thx9zh11	131	16	acids	acid	NNS
cord-328460-thx9zh11	131	17	,	,	,
cord-328460-thx9zh11	131	18	using	use	VBG
cord-328460-thx9zh11	131	19	both	both	CC
cord-328460-thx9zh11	131	20	the	the	DT
cord-328460-thx9zh11	131	21	energy	energy	NN
cord-328460-thx9zh11	131	22	derived	derive	VBN
cord-328460-thx9zh11	131	23	from	from	IN
cord-328460-thx9zh11	131	24	the	the	DT
cord-328460-thx9zh11	131	25	nucleotide	nucleotide	JJ
cord-328460-thx9zh11	131	26	hydrolysis	hydrolysis	NN
cord-328460-thx9zh11	131	27	as	as	RB
cord-328460-thx9zh11	131	28	well	well	RB
cord-328460-thx9zh11	131	29	as	as	IN
cord-328460-thx9zh11	131	30	conformational	conformational	JJ
cord-328460-thx9zh11	131	31	changes	change	NNS
cord-328460-thx9zh11	131	32	that	that	WDT
cord-328460-thx9zh11	131	33	allow	allow	VBP
cord-328460-thx9zh11	131	34	the	the	DT
cord-328460-thx9zh11	131	35	transduction	transduction	NN
cord-328460-thx9zh11	131	36	of	of	IN
cord-328460-thx9zh11	131	37	energy	energy	NN
cord-328460-thx9zh11	131	38	.	.	.
cord-328460-thx9zh11	132	1	Non	non	JJ
cord-328460-thx9zh11	132	2	-	-	JJ
cord-328460-thx9zh11	132	3	conserved	conserved	JJ
cord-328460-thx9zh11	132	4	portions	portion	NNS
cord-328460-thx9zh11	132	5	of	of	IN
cord-328460-thx9zh11	132	6	helicase	helicase	NN
cord-328460-thx9zh11	132	7	structure	structure	NN
cord-328460-thx9zh11	132	8	,	,	,
cord-328460-thx9zh11	132	9	instead	instead	RB
cord-328460-thx9zh11	132	10	,	,	,
cord-328460-thx9zh11	132	11	are	be	VBP
cord-328460-thx9zh11	132	12	likely	likely	JJ
cord-328460-thx9zh11	132	13	to	to	TO
cord-328460-thx9zh11	132	14	encompass	encompass	VB
cord-328460-thx9zh11	132	15	protein	protein	NN
cord-328460-thx9zh11	132	16	-	-	HYPH
cord-328460-thx9zh11	132	17	protein	protein	NN
cord-328460-thx9zh11	132	18	interaction	interaction	NN
cord-328460-thx9zh11	132	19	domains	domain	NNS
cord-328460-thx9zh11	132	20	,	,	,
cord-328460-thx9zh11	132	21	cellular	cellular	JJ
cord-328460-thx9zh11	132	22	localization	localization	NN
cord-328460-thx9zh11	132	23	signals	signal	NNS
cord-328460-thx9zh11	132	24	,	,	,
cord-328460-thx9zh11	132	25	site	site	NN
cord-328460-thx9zh11	132	26	-	-	HYPH
cord-328460-thx9zh11	132	27	specific	specific	JJ
cord-328460-thx9zh11	132	28	DNA	dna	NN
cord-328460-thx9zh11	132	29	recognition	recognition	NN
cord-328460-thx9zh11	132	30	domains	domain	NNS
cord-328460-thx9zh11	132	31	and	and	CC
cord-328460-thx9zh11	132	32	oligomerization	oligomerization	NN
cord-328460-thx9zh11	132	33	interfaces	interface	NNS
cord-328460-thx9zh11	132	34	,	,	,
cord-328460-thx9zh11	132	35	which	which	WDT
cord-328460-thx9zh11	132	36	constitute	constitute	VBP
cord-328460-thx9zh11	132	37	properties	property	NNS
cord-328460-thx9zh11	132	38	that	that	WDT
cord-328460-thx9zh11	132	39	are	be	VBP
cord-328460-thx9zh11	132	40	unique	unique	JJ
cord-328460-thx9zh11	132	41	for	for	IN
cord-328460-thx9zh11	132	42	each	each	DT
cord-328460-thx9zh11	132	43	helicase	helicase	NN
cord-328460-thx9zh11	132	44	[	[	-LRB-
cord-328460-thx9zh11	132	45	65	65	CD
cord-328460-thx9zh11	132	46	]	]	-RRB-
cord-328460-thx9zh11	132	47	.	.	.
cord-328460-thx9zh11	133	1	The	the	DT
cord-328460-thx9zh11	133	2	first	first	JJ
cord-328460-thx9zh11	133	3	HDA	hda	NN
cord-328460-thx9zh11	133	4	system	system	NN
cord-328460-thx9zh11	133	5	was	be	VBD
cord-328460-thx9zh11	133	6	developed	develop	VBN
cord-328460-thx9zh11	133	7	by	by	IN
cord-328460-thx9zh11	133	8	using	use	VBG
cord-328460-thx9zh11	133	9	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	133	10	coli	coli	NNS
cord-328460-thx9zh11	133	11	UvrD	UvrD	NNP
cord-328460-thx9zh11	133	12	helicase	helicase	NNP
cord-328460-thx9zh11	133	13	(	(	-LRB-
cord-328460-thx9zh11	133	14	~82	~82	NNP
cord-328460-thx9zh11	133	15	kDa	kda	XX
cord-328460-thx9zh11	133	16	)	)	-RRB-
cord-328460-thx9zh11	134	1	[	[	-LRB-
cord-328460-thx9zh11	134	2	60	60	CD
cord-328460-thx9zh11	134	3	]	]	-RRB-
cord-328460-thx9zh11	134	4	that	that	WDT
cord-328460-thx9zh11	134	5	can	can	MD
cord-328460-thx9zh11	134	6	operate	operate	VB
cord-328460-thx9zh11	134	7	on	on	IN
cord-328460-thx9zh11	134	8	blunt	blunt	RB
cord-328460-thx9zh11	134	9	-	-	HYPH
cord-328460-thx9zh11	134	10	ended	end	VBN
cord-328460-thx9zh11	134	11	DNA	dna	NN
cord-328460-thx9zh11	134	12	fragments	fragment	NNS
cord-328460-thx9zh11	134	13	as	as	RB
cord-328460-thx9zh11	134	14	well	well	RB
cord-328460-thx9zh11	134	15	as	as	IN
cord-328460-thx9zh11	134	16	nicked	nick	VBN
cord-328460-thx9zh11	134	17	circular	circular	JJ
cord-328460-thx9zh11	134	18	DNA	dna	NN
cord-328460-thx9zh11	134	19	[	[	-LRB-
cord-328460-thx9zh11	134	20	66	66	CD
cord-328460-thx9zh11	134	21	]	]	-RRB-
cord-328460-thx9zh11	134	22	.	.	.
cord-328460-thx9zh11	135	1	UvrD	UvrD	NNP
cord-328460-thx9zh11	135	2	belongs	belong	VBZ
cord-328460-thx9zh11	135	3	to	to	IN
cord-328460-thx9zh11	135	4	the	the	DT
cord-328460-thx9zh11	135	5	superfamily	superfamily	NN
cord-328460-thx9zh11	135	6	1	1	CD
cord-328460-thx9zh11	135	7	(	(	-LRB-
cord-328460-thx9zh11	135	8	SF1	SF1	NNP
cord-328460-thx9zh11	135	9	)	)	-RRB-
cord-328460-thx9zh11	135	10	and	and	CC
cord-328460-thx9zh11	135	11	shows	show	VBZ
cord-328460-thx9zh11	135	12	unidirectionality	unidirectionality	NN
cord-328460-thx9zh11	135	13	in	in	IN
cord-328460-thx9zh11	135	14	dsDNA	dsdna	RB
cord-328460-thx9zh11	135	15	unwinding	unwind	VBG
cord-328460-thx9zh11	135	16	(	(	-LRB-
cord-328460-thx9zh11	135	17	3'-5	3'-5	NNP
cord-328460-thx9zh11	135	18	'	'	''
cord-328460-thx9zh11	135	19	)	)	-RRB-
cord-328460-thx9zh11	135	20	.	.	.
cord-328460-thx9zh11	136	1	In	in	IN
cord-328460-thx9zh11	136	2	the	the	DT
cord-328460-thx9zh11	136	3	absence	absence	NN
cord-328460-thx9zh11	136	4	of	of	IN
cord-328460-thx9zh11	136	5	accessory	accessory	NN
cord-328460-thx9zh11	136	6	proteins	protein	NNS
cord-328460-thx9zh11	136	7	(	(	-LRB-
cord-328460-thx9zh11	136	8	SSB	SSB	NNP
cord-328460-thx9zh11	136	9	.	.	.
cord-328460-thx9zh11	137	1	In	in	IN
cord-328460-thx9zh11	137	2	,	,	,
cord-328460-thx9zh11	137	3	MutL	MutL	NNP
cord-328460-thx9zh11	137	4	and	and	CC
cord-328460-thx9zh11	137	5	T4	T4	NNP
cord-328460-thx9zh11	137	6	gene	gene	NN
cord-328460-thx9zh11	137	7	32	32	CD
cord-328460-thx9zh11	137	8	protein	protein	NN
cord-328460-thx9zh11	137	9	)	)	-RRB-
cord-328460-thx9zh11	137	10	,	,	,
cord-328460-thx9zh11	137	11	no	no	DT
cord-328460-thx9zh11	137	12	amplification	amplification	NN
cord-328460-thx9zh11	137	13	products	product	NNS
cord-328460-thx9zh11	137	14	were	be	VBD
cord-328460-thx9zh11	137	15	produced	produce	VBN
cord-328460-thx9zh11	137	16	.	.	.
cord-328460-thx9zh11	138	1	SSBs	ssb	NNS
cord-328460-thx9zh11	138	2	specifically	specifically	RB
cord-328460-thx9zh11	138	3	bind	bind	VBP
cord-328460-thx9zh11	138	4	to	to	IN
cord-328460-thx9zh11	138	5	the	the	DT
cord-328460-thx9zh11	138	6	single	single	RB
cord-328460-thx9zh11	138	7	-	-	HYPH
cord-328460-thx9zh11	138	8	stranded	strand	VBN
cord-328460-thx9zh11	138	9	part	part	NN
cord-328460-thx9zh11	138	10	of	of	IN
cord-328460-thx9zh11	138	11	DNA	dna	NN
cord-328460-thx9zh11	138	12	in	in	IN
cord-328460-thx9zh11	138	13	a	a	DT
cord-328460-thx9zh11	138	14	sequence	sequence	NN
cord-328460-thx9zh11	138	15	-	-	HYPH
cord-328460-thx9zh11	138	16	independent	independent	JJ
cord-328460-thx9zh11	138	17	manner	manner	NN
cord-328460-thx9zh11	138	18	.	.	.
cord-328460-thx9zh11	139	1	They	-PRON-	PRP
cord-328460-thx9zh11	139	2	enhance	enhance	VBP
cord-328460-thx9zh11	139	3	polymerase	polymerase	NN
cord-328460-thx9zh11	139	4	activity	activity	NN
cord-328460-thx9zh11	139	5	by	by	IN
cord-328460-thx9zh11	139	6	protecting	protect	VBG
cord-328460-thx9zh11	139	7	unwound	unwind	VBN
cord-328460-thx9zh11	139	8	ssDNAs	ssDNAs	NNP
cord-328460-thx9zh11	139	9	from	from	IN
cord-328460-thx9zh11	139	10	degradation	degradation	NN
cord-328460-thx9zh11	139	11	and	and	CC
cord-328460-thx9zh11	139	12	by	by	IN
cord-328460-thx9zh11	139	13	destabilizing	destabilize	VBG
cord-328460-thx9zh11	139	14	DNA	dna	NN
cord-328460-thx9zh11	139	15	secondary	secondary	JJ
cord-328460-thx9zh11	139	16	structure	structure	NN
cord-328460-thx9zh11	139	17	[	[	-LRB-
cord-328460-thx9zh11	139	18	67	67	CD
cord-328460-thx9zh11	139	19	,	,	,
cord-328460-thx9zh11	139	20	68	68	CD
cord-328460-thx9zh11	139	21	]	]	-RRB-
cord-328460-thx9zh11	139	22	.	.	.
cord-328460-thx9zh11	140	1	The	the	DT
cord-328460-thx9zh11	140	2	limited	limited	JJ
cord-328460-thx9zh11	140	3	capability	capability	NN
cord-328460-thx9zh11	140	4	of	of	IN
cord-328460-thx9zh11	140	5	UvrD	UvrD	NNP
cord-328460-thx9zh11	140	6	HDA	hda	NN
cord-328460-thx9zh11	140	7	system	system	NN
cord-328460-thx9zh11	140	8	in	in	IN
cord-328460-thx9zh11	140	9	efficiently	efficiently	RB
cord-328460-thx9zh11	140	10	amplifying	amplify	VBG
cord-328460-thx9zh11	140	11	long	long	JJ
cord-328460-thx9zh11	140	12	target	target	NN
cord-328460-thx9zh11	140	13	sequences	sequence	NNS
cord-328460-thx9zh11	140	14	[	[	-LRB-
cord-328460-thx9zh11	140	15	69	69	CD
cord-328460-thx9zh11	140	16	,	,	,
cord-328460-thx9zh11	140	17	70	70	CD
cord-328460-thx9zh11	140	18	]	]	-RRB-
cord-328460-thx9zh11	140	19	was	be	VBD
cord-328460-thx9zh11	140	20	improved	improve	VBN
cord-328460-thx9zh11	140	21	by	by	IN
cord-328460-thx9zh11	140	22	using	use	VBG
cord-328460-thx9zh11	140	23	a	a	DT
cord-328460-thx9zh11	140	24	thermostable	thermostable	JJ
cord-328460-thx9zh11	140	25	UvrD	UvrD	NNP
cord-328460-thx9zh11	140	26	helicase	helicase	NN
cord-328460-thx9zh11	140	27	(	(	-LRB-
cord-328460-thx9zh11	140	28	Tte	Tte	NNP
cord-328460-thx9zh11	140	29	-	-	HYPH
cord-328460-thx9zh11	140	30	UvrD	UvrD	NNP
cord-328460-thx9zh11	140	31	)	)	-RRB-
cord-328460-thx9zh11	140	32	purified	purify	VBN
cord-328460-thx9zh11	140	33	from	from	IN
cord-328460-thx9zh11	140	34	thermophilic	thermophilic	JJ
cord-328460-thx9zh11	140	35	bacteria	bacteria	NNS
cord-328460-thx9zh11	140	36	and	and	CC
cord-328460-thx9zh11	140	37	the	the	DT
cord-328460-thx9zh11	140	38	Bacillus	bacillus	NN
cord-328460-thx9zh11	140	39	stearothermophilus	stearothermophilus	NN
cord-328460-thx9zh11	140	40	polymerase	polymerase	NN
cord-328460-thx9zh11	141	1	I	-PRON-	PRP
cord-328460-thx9zh11	141	2	at	at	IN
cord-328460-thx9zh11	141	3	60	60	CD
cord-328460-thx9zh11	141	4	-	-	SYM
cord-328460-thx9zh11	141	5	65	65	CD
cord-328460-thx9zh11	141	6	°	°	NN
cord-328460-thx9zh11	141	7	C	c	NN
cord-328460-thx9zh11	141	8	[	[	-LRB-
cord-328460-thx9zh11	141	9	71	71	CD
cord-328460-thx9zh11	141	10	]	]	-RRB-
cord-328460-thx9zh11	141	11	.	.	.
cord-328460-thx9zh11	142	1	Multiple	multiple	JJ
cord-328460-thx9zh11	142	2	reports	report	NNS
cord-328460-thx9zh11	142	3	on	on	IN
cord-328460-thx9zh11	142	4	the	the	DT
cord-328460-thx9zh11	142	5	use	use	NN
cord-328460-thx9zh11	142	6	of	of	IN
cord-328460-thx9zh11	142	7	HDA	hda	NN
cord-328460-thx9zh11	142	8	for	for	IN
cord-328460-thx9zh11	142	9	the	the	DT
cord-328460-thx9zh11	142	10	detection	detection	NN
cord-328460-thx9zh11	142	11	of	of	IN
cord-328460-thx9zh11	142	12	bacterial	bacterial	JJ
cord-328460-thx9zh11	142	13	and	and	CC
cord-328460-thx9zh11	142	14	viral	viral	JJ
cord-328460-thx9zh11	142	15	target	target	NN
cord-328460-thx9zh11	142	16	DNA	DNA	NNP
cord-328460-thx9zh11	142	17	and	and	CC
cord-328460-thx9zh11	142	18	RNA	RNA	NNP
cord-328460-thx9zh11	142	19	in	in	IN
cord-328460-thx9zh11	142	20	standard	standard	JJ
cord-328460-thx9zh11	142	21	microtube	microtube	NN
cord-328460-thx9zh11	142	22	protocols	protocol	NNS
cord-328460-thx9zh11	142	23	have	have	VBP
cord-328460-thx9zh11	142	24	been	be	VBN
cord-328460-thx9zh11	142	25	described	describe	VBN
cord-328460-thx9zh11	142	26	[	[	-LRB-
cord-328460-thx9zh11	142	27	72	72	CD
cord-328460-thx9zh11	142	28	,	,	,
cord-328460-thx9zh11	142	29	73	73	CD
cord-328460-thx9zh11	142	30	]	]	-RRB-
cord-328460-thx9zh11	142	31	.	.	.
cord-328460-thx9zh11	143	1	Recently	recently	RB
cord-328460-thx9zh11	143	2	,	,	,
cord-328460-thx9zh11	143	3	the	the	DT
cord-328460-thx9zh11	143	4	use	use	NN
cord-328460-thx9zh11	143	5	of	of	IN
cord-328460-thx9zh11	143	6	HDA	hda	NN
cord-328460-thx9zh11	143	7	system	system	NN
cord-328460-thx9zh11	143	8	for	for	IN
cord-328460-thx9zh11	143	9	the	the	DT
cord-328460-thx9zh11	143	10	detection	detection	NN
cord-328460-thx9zh11	143	11	of	of	IN
cord-328460-thx9zh11	143	12	antibiotic	antibiotic	JJ
cord-328460-thx9zh11	143	13	-	-	HYPH
cord-328460-thx9zh11	143	14	resistant	resistant	JJ
cord-328460-thx9zh11	143	15	pathogens	pathogen	NNS
cord-328460-thx9zh11	143	16	N.	N.	NNP
cord-328460-thx9zh11	143	17	gonorrhoeae	gonorrhoeae	NNP
cord-328460-thx9zh11	143	18	and	and	CC
cord-328460-thx9zh11	143	19	S.	S.	NNP
cord-328460-thx9zh11	143	20	aureus	aureu	NNS
cord-328460-thx9zh11	143	21	directly	directly	RB
cord-328460-thx9zh11	143	22	on	on	IN
cord-328460-thx9zh11	143	23	surface	surface	NN
cord-328460-thx9zh11	143	24	bound	bind	VBN
cord-328460-thx9zh11	143	25	primers	primer	NNS
cord-328460-thx9zh11	143	26	has	have	VBZ
cord-328460-thx9zh11	143	27	been	be	VBN
cord-328460-thx9zh11	143	28	reported	report	VBN
cord-328460-thx9zh11	143	29	[	[	-LRB-
cord-328460-thx9zh11	143	30	74	74	CD
cord-328460-thx9zh11	143	31	]	]	-RRB-
cord-328460-thx9zh11	143	32	.	.	.
cord-328460-thx9zh11	144	1	It	-PRON-	PRP
cord-328460-thx9zh11	144	2	combines	combine	VBZ
cord-328460-thx9zh11	144	3	HDA	hda	NN
cord-328460-thx9zh11	144	4	with	with	IN
cord-328460-thx9zh11	144	5	a	a	DT
cord-328460-thx9zh11	144	6	microarray	microarray	NN
cord-328460-thx9zh11	144	7	-	-	HYPH
cord-328460-thx9zh11	144	8	based	base	VBN
cord-328460-thx9zh11	144	9	detection	detection	NN
cord-328460-thx9zh11	144	10	,	,	,
cord-328460-thx9zh11	144	11	making	make	VBG
cord-328460-thx9zh11	144	12	it	-PRON-	PRP
cord-328460-thx9zh11	144	13	suitable	suitable	JJ
cord-328460-thx9zh11	144	14	for	for	IN
cord-328460-thx9zh11	144	15	multiplex	multiplex	JJ
cord-328460-thx9zh11	144	16	detection	detection	NN
cord-328460-thx9zh11	144	17	.	.	.
cord-328460-thx9zh11	145	1	The	the	DT
cord-328460-thx9zh11	145	2	helicase	helicase	NN
cord-328460-thx9zh11	145	3	dependent	dependent	JJ
cord-328460-thx9zh11	145	4	OnChip	onchip	NN
cord-328460-thx9zh11	145	5	amplification	amplification	NN
cord-328460-thx9zh11	145	6	(	(	-LRB-
cord-328460-thx9zh11	145	7	OnChip	onchip	NN
cord-328460-thx9zh11	145	8	-	-	HYPH
cord-328460-thx9zh11	145	9	HDA	HDA	NNP
cord-328460-thx9zh11	145	10	)	)	-RRB-
cord-328460-thx9zh11	145	11	provides	provide	VBZ
cord-328460-thx9zh11	145	12	a	a	DT
cord-328460-thx9zh11	145	13	way	way	NN
cord-328460-thx9zh11	145	14	for	for	IN
cord-328460-thx9zh11	145	15	HDA	hda	NN
cord-328460-thx9zh11	145	16	to	to	TO
cord-328460-thx9zh11	145	17	be	be	VB
cord-328460-thx9zh11	145	18	developed	develop	VBN
cord-328460-thx9zh11	145	19	into	into	IN
cord-328460-thx9zh11	145	20	a	a	DT
cord-328460-thx9zh11	145	21	miniaturized	miniaturize	VBN
cord-328460-thx9zh11	145	22	detection	detection	NN
cord-328460-thx9zh11	145	23	system	system	NN
cord-328460-thx9zh11	145	24	for	for	IN
cord-328460-thx9zh11	145	25	point	point	NN
cord-328460-thx9zh11	145	26	-	-	HYPH
cord-328460-thx9zh11	145	27	of	of	IN
cord-328460-thx9zh11	145	28	-	-	HYPH
cord-328460-thx9zh11	145	29	care	care	NN
cord-328460-thx9zh11	145	30	diagnosis	diagnosis	NN
cord-328460-thx9zh11	145	31	in	in	IN
cord-328460-thx9zh11	145	32	conjunction	conjunction	NN
cord-328460-thx9zh11	145	33	with	with	IN
cord-328460-thx9zh11	145	34	commercially	commercially	RB
cord-328460-thx9zh11	145	35	available	available	JJ
cord-328460-thx9zh11	145	36	thermostable	thermostable	JJ
cord-328460-thx9zh11	145	37	HDA	hda	NN
cord-328460-thx9zh11	145	38	kits	kit	NNS
cord-328460-thx9zh11	145	39	[	[	-LRB-
cord-328460-thx9zh11	145	40	75	75	CD
cord-328460-thx9zh11	145	41	]	]	-RRB-
cord-328460-thx9zh11	145	42	.	.	.
cord-328460-thx9zh11	146	1	Ramalingam	Ramalingam	NNP
cord-328460-thx9zh11	146	2	et	et	NNP
cord-328460-thx9zh11	146	3	al	al	NNP
cord-328460-thx9zh11	146	4	.	.	.
cord-328460-thx9zh11	147	1	[	[	-LRB-
cord-328460-thx9zh11	147	2	76	76	CD
cord-328460-thx9zh11	147	3	]	]	-RRB-
cord-328460-thx9zh11	147	4	described	describe	VBD
cord-328460-thx9zh11	147	5	a	a	DT
cord-328460-thx9zh11	147	6	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	147	7	devices	device	NNS
cord-328460-thx9zh11	147	8	with	with	IN
cord-328460-thx9zh11	147	9	an	an	DT
cord-328460-thx9zh11	147	10	array	array	NN
cord-328460-thx9zh11	147	11	of	of	IN
cord-328460-thx9zh11	147	12	open	open	JJ
cord-328460-thx9zh11	147	13	(	(	-LRB-
cord-328460-thx9zh11	147	14	unsealed	unsealed	JJ
cord-328460-thx9zh11	147	15	)	)	-RRB-
cord-328460-thx9zh11	147	16	reactors	reactor	NNS
cord-328460-thx9zh11	147	17	preloaded	preloade	VBN
cord-328460-thx9zh11	147	18	with	with	IN
cord-328460-thx9zh11	147	19	primers	primer	NNS
cord-328460-thx9zh11	147	20	in	in	IN
cord-328460-thx9zh11	147	21	conjunction	conjunction	NN
cord-328460-thx9zh11	147	22	with	with	IN
cord-328460-thx9zh11	147	23	a	a	DT
cord-328460-thx9zh11	147	24	single	single	JJ
cord-328460-thx9zh11	147	25	-	-	HYPH
cord-328460-thx9zh11	147	26	step	step	NN
cord-328460-thx9zh11	147	27	capillary	capillary	NN
cord-328460-thx9zh11	147	28	-	-	HYPH
cord-328460-thx9zh11	147	29	based	base	VBN
cord-328460-thx9zh11	147	30	flow	flow	NN
cord-328460-thx9zh11	147	31	scheme	scheme	NN
cord-328460-thx9zh11	147	32	for	for	IN
cord-328460-thx9zh11	147	33	sequential	sequential	JJ
cord-328460-thx9zh11	147	34	distribution	distribution	NN
cord-328460-thx9zh11	147	35	of	of	IN
cord-328460-thx9zh11	147	36	the	the	DT
cord-328460-thx9zh11	147	37	amplification	amplification	NN
cord-328460-thx9zh11	147	38	mixture	mixture	NN
cord-328460-thx9zh11	147	39	and	and	CC
cord-328460-thx9zh11	147	40	HDA	hda	NN
cord-328460-thx9zh11	147	41	isothermal	isothermal	JJ
cord-328460-thx9zh11	147	42	amplification	amplification	NN
cord-328460-thx9zh11	147	43	of	of	IN
cord-328460-thx9zh11	147	44	nucleic	nucleic	NNS
cord-328460-thx9zh11	147	45	-	-	HYPH
cord-328460-thx9zh11	147	46	acid	acid	NN
cord-328460-thx9zh11	147	47	.	.	.
cord-328460-thx9zh11	148	1	In	in	IN
cord-328460-thx9zh11	148	2	this	this	DT
cord-328460-thx9zh11	148	3	case	case	NN
cord-328460-thx9zh11	148	4	,	,	,
cord-328460-thx9zh11	148	5	the	the	DT
cord-328460-thx9zh11	148	6	BNI-1	BNI-1	NNP
cord-328460-thx9zh11	148	7	fragment	fragment	NN
cord-328460-thx9zh11	148	8	of	of	IN
cord-328460-thx9zh11	148	9	SARS	SARS	NNP
cord-328460-thx9zh11	148	10	cDNA	cdna	NN
cord-328460-thx9zh11	148	11	was	be	VBD
cord-328460-thx9zh11	148	12	successfully	successfully	RB
cord-328460-thx9zh11	148	13	amplified	amplify	VBN
cord-328460-thx9zh11	148	14	in	in	IN
cord-328460-thx9zh11	148	15	a	a	DT
cord-328460-thx9zh11	148	16	real	real	JJ
cord-328460-thx9zh11	148	17	-	-	HYPH
cord-328460-thx9zh11	148	18	time	time	NN
cord-328460-thx9zh11	148	19	format	format	NN
cord-328460-thx9zh11	148	20	at	at	IN
cord-328460-thx9zh11	148	21	62	62	CD
cord-328460-thx9zh11	148	22	°	°	NN
cord-328460-thx9zh11	148	23	C	c	NN
cord-328460-thx9zh11	148	24	by	by	IN
cord-328460-thx9zh11	148	25	using	use	VBG
cord-328460-thx9zh11	148	26	the	the	DT
cord-328460-thx9zh11	148	27	IsoAmp	IsoAmp	NNP
cord-328460-thx9zh11	148	28	tHDA	thda	NN
cord-328460-thx9zh11	148	29	kit	kit	NN
cord-328460-thx9zh11	148	30	(	(	-LRB-
cord-328460-thx9zh11	148	31	New	New	NNP
cord-328460-thx9zh11	148	32	England	England	NNP
cord-328460-thx9zh11	148	33	Biolabs	Biolabs	NNP
cord-328460-thx9zh11	148	34	,	,	,
cord-328460-thx9zh11	148	35	Beverly	Beverly	NNP
cord-328460-thx9zh11	148	36	,	,	,
cord-328460-thx9zh11	148	37	MA	MA	NNP
cord-328460-thx9zh11	148	38	,	,	,
cord-328460-thx9zh11	148	39	USA	USA	NNP
cord-328460-thx9zh11	148	40	)	)	-RRB-
cord-328460-thx9zh11	148	41	containing	contain	VBG
cord-328460-thx9zh11	148	42	thermostable	thermostable	JJ
cord-328460-thx9zh11	148	43	UvrD	UvrD	NNP
cord-328460-thx9zh11	148	44	helicase	helicase	NN
cord-328460-thx9zh11	148	45	(	(	-LRB-
cord-328460-thx9zh11	148	46	Tte	Tte	NNP
cord-328460-thx9zh11	148	47	-	-	HYPH
cord-328460-thx9zh11	148	48	UvrD	UvrD	NNP
cord-328460-thx9zh11	148	49	)	)	-RRB-
cord-328460-thx9zh11	148	50	and	and	CC
cord-328460-thx9zh11	148	51	thermostable	thermostable	JJ
cord-328460-thx9zh11	148	52	DNA	dna	NN
cord-328460-thx9zh11	148	53	polymerase	polymerase	NN
cord-328460-thx9zh11	148	54	.	.	.
cord-328460-thx9zh11	149	1	As	as	IN
cord-328460-thx9zh11	149	2	a	a	DT
cord-328460-thx9zh11	149	3	consequence	consequence	NN
cord-328460-thx9zh11	149	4	of	of	IN
cord-328460-thx9zh11	149	5	the	the	DT
cord-328460-thx9zh11	149	6	limited	limited	JJ
cord-328460-thx9zh11	149	7	speed	speed	NN
cord-328460-thx9zh11	149	8	and	and	CC
cord-328460-thx9zh11	149	9	processivity	processivity	NN
cord-328460-thx9zh11	149	10	of	of	IN
cord-328460-thx9zh11	149	11	the	the	DT
cord-328460-thx9zh11	149	12	UvrD	UvrD	NNP
cord-328460-thx9zh11	149	13	helicase	helicase	NN
cord-328460-thx9zh11	149	14	,	,	,
cord-328460-thx9zh11	149	15	the	the	DT
cord-328460-thx9zh11	149	16	system	system	NN
cord-328460-thx9zh11	149	17	was	be	VBD
cord-328460-thx9zh11	149	18	able	able	JJ
cord-328460-thx9zh11	149	19	to	to	TO
cord-328460-thx9zh11	149	20	amplify	amplify	VB
cord-328460-thx9zh11	149	21	only	only	JJ
cord-328460-thx9zh11	149	22	short	short	JJ
cord-328460-thx9zh11	149	23	DNA	dna	NN
cord-328460-thx9zh11	149	24	sequence	sequence	NN
cord-328460-thx9zh11	149	25	(	(	-LRB-
cord-328460-thx9zh11	149	26	from	from	IN
cord-328460-thx9zh11	149	27	70	70	CD
cord-328460-thx9zh11	149	28	to	to	TO
cord-328460-thx9zh11	149	29	120	120	CD
cord-328460-thx9zh11	149	30	bp	bp	NNS
cord-328460-thx9zh11	149	31	)	)	-RRB-
cord-328460-thx9zh11	149	32	[	[	-LRB-
cord-328460-thx9zh11	149	33	71	71	CD
cord-328460-thx9zh11	149	34	]	]	-RRB-
cord-328460-thx9zh11	149	35	.	.	.
cord-328460-thx9zh11	150	1	It	-PRON-	PRP
cord-328460-thx9zh11	150	2	has	have	VBZ
cord-328460-thx9zh11	150	3	been	be	VBN
cord-328460-thx9zh11	150	4	reported	report	VBN
cord-328460-thx9zh11	150	5	that	that	IN
cord-328460-thx9zh11	150	6	the	the	DT
cord-328460-thx9zh11	150	7	speed	speed	NN
cord-328460-thx9zh11	150	8	and	and	CC
cord-328460-thx9zh11	150	9	the	the	DT
cord-328460-thx9zh11	150	10	robustness	robustness	NN
cord-328460-thx9zh11	150	11	of	of	IN
cord-328460-thx9zh11	150	12	HDA	HDA	NNP
cord-328460-thx9zh11	150	13	are	be	VBP
cord-328460-thx9zh11	150	14	highly	highly	RB
cord-328460-thx9zh11	150	15	dependent	dependent	JJ
cord-328460-thx9zh11	150	16	on	on	IN
cord-328460-thx9zh11	150	17	the	the	DT
cord-328460-thx9zh11	150	18	synchronization	synchronization	NN
cord-328460-thx9zh11	150	19	of	of	IN
cord-328460-thx9zh11	150	20	helicase	helicase	NN
cord-328460-thx9zh11	150	21	,	,	,
cord-328460-thx9zh11	150	22	single	single	RB
cord-328460-thx9zh11	150	23	-	-	HYPH
cord-328460-thx9zh11	150	24	stranded	strand	VBN
cord-328460-thx9zh11	150	25	DNA	dna	NN
cord-328460-thx9zh11	150	26	binding	bind	VBG
cord-328460-thx9zh11	150	27	protein	protein	NN
cord-328460-thx9zh11	150	28	and	and	CC
cord-328460-thx9zh11	150	29	large	large	JJ
cord-328460-thx9zh11	150	30	fragment	fragment	NN
cord-328460-thx9zh11	150	31	of	of	IN
cord-328460-thx9zh11	150	32	Bst	Bst	NNP
cord-328460-thx9zh11	150	33	DNA	dna	NN
cord-328460-thx9zh11	150	34	polymerase	polymerase	NN
cord-328460-thx9zh11	150	35	[	[	-LRB-
cord-328460-thx9zh11	150	36	77	77	CD
cord-328460-thx9zh11	150	37	]	]	-RRB-
cord-328460-thx9zh11	150	38	.	.	.
cord-328460-thx9zh11	151	1	By	by	IN
cord-328460-thx9zh11	151	2	increasing	increase	VBG
cord-328460-thx9zh11	151	3	the	the	DT
cord-328460-thx9zh11	151	4	concentration	concentration	NN
cord-328460-thx9zh11	151	5	of	of	IN
cord-328460-thx9zh11	151	6	the	the	DT
cord-328460-thx9zh11	151	7	constituent	constituent	NN
cord-328460-thx9zh11	151	8	enzymes	enzyme	NNS
cord-328460-thx9zh11	151	9	,	,	,
cord-328460-thx9zh11	151	10	while	while	IN
cord-328460-thx9zh11	151	11	maintaining	maintain	VBG
cord-328460-thx9zh11	151	12	the	the	DT
cord-328460-thx9zh11	151	13	relative	relative	JJ
cord-328460-thx9zh11	151	14	proportions	proportion	NNS
cord-328460-thx9zh11	151	15	of	of	IN
cord-328460-thx9zh11	151	16	each	each	DT
cord-328460-thx9zh11	151	17	type	type	NN
cord-328460-thx9zh11	151	18	of	of	IN
cord-328460-thx9zh11	151	19	enzyme	enzyme	NN
cord-328460-thx9zh11	151	20	constant	constant	NN
cord-328460-thx9zh11	151	21	,	,	,
cord-328460-thx9zh11	151	22	the	the	DT
cord-328460-thx9zh11	151	23	overall	overall	JJ
cord-328460-thx9zh11	151	24	reaction	reaction	NN
cord-328460-thx9zh11	151	25	speed	speed	NN
cord-328460-thx9zh11	151	26	has	have	VBZ
cord-328460-thx9zh11	151	27	been	be	VBN
cord-328460-thx9zh11	151	28	increased	increase	VBN
cord-328460-thx9zh11	151	29	.	.	.
cord-328460-thx9zh11	152	1	The	the	DT
cord-328460-thx9zh11	152	2	increasing	increase	VBG
cord-328460-thx9zh11	152	3	of	of	IN
cord-328460-thx9zh11	152	4	the	the	DT
cord-328460-thx9zh11	152	5	primer	primer	NN
cord-328460-thx9zh11	152	6	concentration	concentration	NN
cord-328460-thx9zh11	152	7	also	also	RB
cord-328460-thx9zh11	152	8	improved	improve	VBD
cord-328460-thx9zh11	152	9	the	the	DT
cord-328460-thx9zh11	152	10	reaction	reaction	NN
cord-328460-thx9zh11	152	11	speed	speed	NN
cord-328460-thx9zh11	152	12	but	but	CC
cord-328460-thx9zh11	152	13	also	also	RB
cord-328460-thx9zh11	152	14	produced	produce	VBD
cord-328460-thx9zh11	152	15	a	a	DT
cord-328460-thx9zh11	152	16	partial	partial	JJ
cord-328460-thx9zh11	152	17	loss	loss	NN
cord-328460-thx9zh11	152	18	in	in	IN
cord-328460-thx9zh11	152	19	amplification	amplification	NN
cord-328460-thx9zh11	152	20	accuracy	accuracy	NN
cord-328460-thx9zh11	152	21	.	.	.
cord-328460-thx9zh11	153	1	The	the	DT
cord-328460-thx9zh11	153	2	integration	integration	NN
cord-328460-thx9zh11	153	3	in	in	IN
cord-328460-thx9zh11	153	4	a	a	DT
cord-328460-thx9zh11	153	5	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	153	6	chip	chip	NN
cord-328460-thx9zh11	153	7	of	of	IN
cord-328460-thx9zh11	153	8	isothermal	isothermal	JJ
cord-328460-thx9zh11	153	9	amplification	amplification	NN
cord-328460-thx9zh11	153	10	by	by	IN
cord-328460-thx9zh11	153	11	HDA	HDA	NNP
cord-328460-thx9zh11	153	12	and	and	CC
cord-328460-thx9zh11	153	13	micro	micro	JJ
cord-328460-thx9zh11	153	14	solid	solid	JJ
cord-328460-thx9zh11	153	15	phase	phase	NN
cord-328460-thx9zh11	153	16	extraction	extraction	NN
cord-328460-thx9zh11	153	17	(	(	-LRB-
cord-328460-thx9zh11	153	18	μSPE	μspe	NN
cord-328460-thx9zh11	153	19	)	)	-RRB-
cord-328460-thx9zh11	153	20	column	column	NN
cord-328460-thx9zh11	153	21	for	for	IN
cord-328460-thx9zh11	153	22	DNA	dna	NN
cord-328460-thx9zh11	153	23	isolation	isolation	NN
cord-328460-thx9zh11	153	24	has	have	VBZ
cord-328460-thx9zh11	153	25	been	be	VBN
cord-328460-thx9zh11	153	26	also	also	RB
cord-328460-thx9zh11	153	27	described	describe	VBN
cord-328460-thx9zh11	153	28	[	[	-LRB-
cord-328460-thx9zh11	153	29	78	78	CD
cord-328460-thx9zh11	153	30	]	]	-RRB-
cord-328460-thx9zh11	153	31	.	.	.
cord-328460-thx9zh11	154	1	In	in	IN
cord-328460-thx9zh11	154	2	this	this	DT
cord-328460-thx9zh11	154	3	case	case	NN
cord-328460-thx9zh11	154	4	,	,	,
cord-328460-thx9zh11	154	5	the	the	DT
cord-328460-thx9zh11	154	6	bacteria	bacteria	NNS
cord-328460-thx9zh11	154	7	lysis	lysis	NN
cord-328460-thx9zh11	154	8	,	,	,
cord-328460-thx9zh11	154	9	the	the	DT
cord-328460-thx9zh11	154	10	nucleic	nucleic	JJ
cord-328460-thx9zh11	154	11	acid	acid	NN
cord-328460-thx9zh11	154	12	extraction	extraction	NN
cord-328460-thx9zh11	154	13	and	and	CC
cord-328460-thx9zh11	154	14	DNA	dna	NN
cord-328460-thx9zh11	154	15	amplification	amplification	NN
cord-328460-thx9zh11	154	16	with	with	IN
cord-328460-thx9zh11	154	17	a	a	DT
cord-328460-thx9zh11	154	18	fluorescent	fluorescent	JJ
cord-328460-thx9zh11	154	19	reporter	reporter	NN
cord-328460-thx9zh11	154	20	were	be	VBD
cord-328460-thx9zh11	154	21	incorporated	incorporate	VBN
cord-328460-thx9zh11	154	22	into	into	IN
cord-328460-thx9zh11	154	23	a	a	DT
cord-328460-thx9zh11	154	24	disposable	disposable	JJ
cord-328460-thx9zh11	154	25	polymer	polymer	NN
cord-328460-thx9zh11	154	26	cartridge	cartridge	NN
cord-328460-thx9zh11	154	27	to	to	TO
cord-328460-thx9zh11	154	28	detect	detect	VB
cord-328460-thx9zh11	154	29	the	the	DT
cord-328460-thx9zh11	154	30	presence	presence	NN
cord-328460-thx9zh11	154	31	of	of	IN
cord-328460-thx9zh11	154	32	whole	whole	JJ
cord-328460-thx9zh11	154	33	bacterial	bacterial	JJ
cord-328460-thx9zh11	154	34	cells	cell	NNS
cord-328460-thx9zh11	154	35	in	in	IN
cord-328460-thx9zh11	154	36	a	a	DT
cord-328460-thx9zh11	154	37	liquid	liquid	JJ
cord-328460-thx9zh11	154	38	samples	sample	NNS
cord-328460-thx9zh11	154	39	.	.	.
cord-328460-thx9zh11	155	1	The	the	DT
cord-328460-thx9zh11	155	2	rolling	rolling	JJ
cord-328460-thx9zh11	155	3	circle	circle	NN
cord-328460-thx9zh11	155	4	amplification	amplification	NN
cord-328460-thx9zh11	155	5	(	(	-LRB-
cord-328460-thx9zh11	155	6	RCA	RCA	NNP
cord-328460-thx9zh11	155	7	)	)	-RRB-
cord-328460-thx9zh11	155	8	method	method	NN
cord-328460-thx9zh11	155	9	exploits	exploit	VBZ
cord-328460-thx9zh11	155	10	the	the	DT
cord-328460-thx9zh11	155	11	continuous	continuous	JJ
cord-328460-thx9zh11	155	12	amplification	amplification	NN
cord-328460-thx9zh11	155	13	of	of	IN
cord-328460-thx9zh11	155	14	a	a	DT
cord-328460-thx9zh11	155	15	circular	circular	JJ
cord-328460-thx9zh11	155	16	DNA	dna	NN
cord-328460-thx9zh11	155	17	template	template	NN
cord-328460-thx9zh11	155	18	by	by	IN
cord-328460-thx9zh11	155	19	a	a	DT
cord-328460-thx9zh11	155	20	strand	strand	NN
cord-328460-thx9zh11	155	21	displacing	displace	VBG
cord-328460-thx9zh11	155	22	DNA	dna	NN
cord-328460-thx9zh11	155	23	polymerase	polymerase	NN
cord-328460-thx9zh11	155	24	.	.	.
cord-328460-thx9zh11	156	1	The	the	DT
cord-328460-thx9zh11	156	2	DNA	dna	NN
cord-328460-thx9zh11	156	3	polymerase	polymerase	NN
cord-328460-thx9zh11	156	4	displaces	displace	VBZ
cord-328460-thx9zh11	156	5	the	the	DT
cord-328460-thx9zh11	156	6	synthesized	synthesize	VBN
cord-328460-thx9zh11	156	7	strand	strand	NN
cord-328460-thx9zh11	156	8	and	and	CC
cord-328460-thx9zh11	156	9	'	'	``
cord-328460-thx9zh11	156	10	rolls	roll	NNS
cord-328460-thx9zh11	156	11	'	'	''
cord-328460-thx9zh11	156	12	on	on	RB
cord-328460-thx9zh11	156	13	with	with	IN
cord-328460-thx9zh11	156	14	DNA	dna	NN
cord-328460-thx9zh11	156	15	synthesis	synthesis	NN
cord-328460-thx9zh11	156	16	.	.	.
cord-328460-thx9zh11	157	1	This	this	DT
cord-328460-thx9zh11	157	2	method	method	NN
cord-328460-thx9zh11	157	3	operates	operate	VBZ
cord-328460-thx9zh11	157	4	at	at	IN
cord-328460-thx9zh11	157	5	a	a	DT
cord-328460-thx9zh11	157	6	constant	constant	JJ
cord-328460-thx9zh11	157	7	temperature	temperature	NN
cord-328460-thx9zh11	157	8	and	and	CC
cord-328460-thx9zh11	157	9	produces	produce	VBZ
cord-328460-thx9zh11	157	10	a	a	DT
cord-328460-thx9zh11	157	11	long	long	JJ
cord-328460-thx9zh11	157	12	single	single	JJ
cord-328460-thx9zh11	157	13	-	-	HYPH
cord-328460-thx9zh11	157	14	stranded	strand	VBN
cord-328460-thx9zh11	157	15	DNA	dna	NN
cord-328460-thx9zh11	157	16	molecule	molecule	NN
cord-328460-thx9zh11	157	17	with	with	IN
cord-328460-thx9zh11	157	18	tandem	tandem	NN
cord-328460-thx9zh11	157	19	repeats	repeat	NNS
cord-328460-thx9zh11	157	20	of	of	IN
cord-328460-thx9zh11	157	21	the	the	DT
cord-328460-thx9zh11	157	22	circular	circular	JJ
cord-328460-thx9zh11	157	23	template	template	NN
cord-328460-thx9zh11	157	24	[	[	-LRB-
cord-328460-thx9zh11	157	25	79	79	CD
cord-328460-thx9zh11	157	26	]	]	-RRB-
cord-328460-thx9zh11	157	27	.	.	.
cord-328460-thx9zh11	158	1	Both	both	DT
cord-328460-thx9zh11	158	2	linear	linear	JJ
cord-328460-thx9zh11	158	3	and	and	CC
cord-328460-thx9zh11	158	4	exponential	exponential	JJ
cord-328460-thx9zh11	158	5	RCAs	rca	NNS
cord-328460-thx9zh11	158	6	have	have	VBP
cord-328460-thx9zh11	158	7	been	be	VBN
cord-328460-thx9zh11	158	8	developed	develop	VBN
cord-328460-thx9zh11	158	9	.	.	.
cord-328460-thx9zh11	159	1	In	in	IN
cord-328460-thx9zh11	159	2	linear	linear	JJ
cord-328460-thx9zh11	159	3	RCA	RCA	NNP
cord-328460-thx9zh11	159	4	,	,	,
cord-328460-thx9zh11	159	5	a	a	DT
cord-328460-thx9zh11	159	6	small	small	JJ
cord-328460-thx9zh11	159	7	circle	circle	NN
cord-328460-thx9zh11	159	8	sequence	sequence	NN
cord-328460-thx9zh11	159	9	is	be	VBZ
cord-328460-thx9zh11	159	10	amplified	amplify	VBN
cord-328460-thx9zh11	159	11	by	by	IN
cord-328460-thx9zh11	159	12	polymerase	polymerase	NN
cord-328460-thx9zh11	159	13	extension	extension	NN
cord-328460-thx9zh11	159	14	of	of	IN
cord-328460-thx9zh11	159	15	a	a	DT
cord-328460-thx9zh11	159	16	complementary	complementary	JJ
cord-328460-thx9zh11	159	17	primer	primer	NN
cord-328460-thx9zh11	159	18	,	,	,
cord-328460-thx9zh11	159	19	whereas	whereas	IN
cord-328460-thx9zh11	159	20	in	in	IN
cord-328460-thx9zh11	159	21	exponential	exponential	JJ
cord-328460-thx9zh11	159	22	RCA	RCA	NNP
cord-328460-thx9zh11	159	23	two	two	CD
cord-328460-thx9zh11	159	24	primers	primer	NNS
cord-328460-thx9zh11	159	25	are	be	VBP
cord-328460-thx9zh11	159	26	used	use	VBN
cord-328460-thx9zh11	159	27	:	:	:
cord-328460-thx9zh11	159	28	the	the	DT
cord-328460-thx9zh11	159	29	second	second	JJ
cord-328460-thx9zh11	159	30	primer	primer	NN
cord-328460-thx9zh11	159	31	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	159	32	with	with	IN
cord-328460-thx9zh11	159	33	the	the	DT
cord-328460-thx9zh11	159	34	single	single	RB
cord-328460-thx9zh11	159	35	-	-	HYPH
cord-328460-thx9zh11	159	36	stranded	strand	VBN
cord-328460-thx9zh11	159	37	DNA	dna	NN
cord-328460-thx9zh11	159	38	product	product	NN
cord-328460-thx9zh11	159	39	of	of	IN
cord-328460-thx9zh11	159	40	the	the	DT
cord-328460-thx9zh11	159	41	first	first	JJ
cord-328460-thx9zh11	159	42	primer	primer	NN
cord-328460-thx9zh11	159	43	and	and	CC
cord-328460-thx9zh11	159	44	initiates	initiate	VBZ
cord-328460-thx9zh11	159	45	hyper	hyper	RB
cord-328460-thx9zh11	159	46	-	-	NN
cord-328460-thx9zh11	159	47	branching	branch	VBG
cord-328460-thx9zh11	159	48	in	in	IN
cord-328460-thx9zh11	159	49	the	the	DT
cord-328460-thx9zh11	159	50	DNA	dna	NN
cord-328460-thx9zh11	159	51	replication	replication	NN
cord-328460-thx9zh11	159	52	[	[	-LRB-
cord-328460-thx9zh11	159	53	80	80	CD
cord-328460-thx9zh11	159	54	]	]	-RRB-
cord-328460-thx9zh11	159	55	.	.	.
cord-328460-thx9zh11	160	1	A	a	DT
cord-328460-thx9zh11	160	2	schematic	schematic	JJ
cord-328460-thx9zh11	160	3	illustration	illustration	NN
cord-328460-thx9zh11	160	4	of	of	IN
cord-328460-thx9zh11	160	5	the	the	DT
cord-328460-thx9zh11	160	6	principle	principle	NN
cord-328460-thx9zh11	160	7	of	of	IN
cord-328460-thx9zh11	160	8	rolling	rolling	JJ
cord-328460-thx9zh11	160	9	-	-	HYPH
cord-328460-thx9zh11	160	10	circle	circle	NN
cord-328460-thx9zh11	160	11	amplification	amplification	NN
cord-328460-thx9zh11	160	12	is	be	VBZ
cord-328460-thx9zh11	160	13	shown	show	VBN
cord-328460-thx9zh11	160	14	in	in	IN
cord-328460-thx9zh11	160	15	Figure	Figure	NNP
cord-328460-thx9zh11	160	16	3	3	CD
cord-328460-thx9zh11	160	17	.	.	.
cord-328460-thx9zh11	161	1	Efficient	efficient	JJ
cord-328460-thx9zh11	161	2	amplification	amplification	NN
cord-328460-thx9zh11	161	3	by	by	IN
cord-328460-thx9zh11	161	4	RCA	RCA	NNP
cord-328460-thx9zh11	161	5	requires	require	VBZ
cord-328460-thx9zh11	161	6	small	small	JJ
cord-328460-thx9zh11	161	7	and	and	CC
cord-328460-thx9zh11	161	8	circular	circular	JJ
cord-328460-thx9zh11	161	9	single	single	RB
cord-328460-thx9zh11	161	10	-	-	HYPH
cord-328460-thx9zh11	161	11	stranded	strand	VBN
cord-328460-thx9zh11	161	12	DNAs	dna	NNS
cord-328460-thx9zh11	161	13	acting	act	VBG
cord-328460-thx9zh11	161	14	as	as	IN
cord-328460-thx9zh11	161	15	the	the	DT
cord-328460-thx9zh11	161	16	template	template	NN
cord-328460-thx9zh11	161	17	.	.	.
cord-328460-thx9zh11	162	1	Unfortunately	unfortunately	RB
cord-328460-thx9zh11	162	2	,	,	,
cord-328460-thx9zh11	162	3	a	a	DT
cord-328460-thx9zh11	162	4	large	large	JJ
cord-328460-thx9zh11	162	5	number	number	NN
cord-328460-thx9zh11	162	6	of	of	IN
cord-328460-thx9zh11	162	7	relevant	relevant	JJ
cord-328460-thx9zh11	162	8	diagnostic	diagnostic	JJ
cord-328460-thx9zh11	162	9	DNA	dna	NN
cord-328460-thx9zh11	162	10	targets	target	NNS
cord-328460-thx9zh11	162	11	are	be	VBP
cord-328460-thx9zh11	162	12	composed	compose	VBN
cord-328460-thx9zh11	162	13	of	of	IN
cord-328460-thx9zh11	162	14	double	double	JJ
cord-328460-thx9zh11	162	15	stranded	strand	VBN
cord-328460-thx9zh11	162	16	linear	linear	JJ
cord-328460-thx9zh11	162	17	DNA	dna	NN
cord-328460-thx9zh11	162	18	molecules	molecule	NNS
cord-328460-thx9zh11	162	19	thus	thus	RB
cord-328460-thx9zh11	162	20	making	make	VBG
cord-328460-thx9zh11	162	21	it	-PRON-	PRP
cord-328460-thx9zh11	162	22	difficult	difficult	JJ
cord-328460-thx9zh11	162	23	the	the	DT
cord-328460-thx9zh11	162	24	amplification	amplification	NN
cord-328460-thx9zh11	162	25	process	process	NN
cord-328460-thx9zh11	162	26	to	to	TO
cord-328460-thx9zh11	162	27	be	be	VB
cord-328460-thx9zh11	162	28	established	establish	VBN
cord-328460-thx9zh11	162	29	.	.	.
cord-328460-thx9zh11	163	1	For	for	IN
cord-328460-thx9zh11	163	2	this	this	DT
cord-328460-thx9zh11	163	3	reason	reason	NN
cord-328460-thx9zh11	163	4	,	,	,
cord-328460-thx9zh11	163	5	oligonucleotides	oligonucleotide	NNS
cord-328460-thx9zh11	163	6	called	call	VBD
cord-328460-thx9zh11	163	7	padlock	padlock	NN
cord-328460-thx9zh11	163	8	probes	probe	NNS
cord-328460-thx9zh11	163	9	have	have	VBP
cord-328460-thx9zh11	163	10	been	be	VBN
cord-328460-thx9zh11	163	11	designed	design	VBN
cord-328460-thx9zh11	163	12	[	[	-LRB-
cord-328460-thx9zh11	163	13	81	81	CD
cord-328460-thx9zh11	163	14	]	]	-RRB-
cord-328460-thx9zh11	163	15	.	.	.
cord-328460-thx9zh11	164	1	Padlock	padlock	NN
cord-328460-thx9zh11	164	2	probes	probe	NNS
cord-328460-thx9zh11	164	3	are	be	VBP
cord-328460-thx9zh11	164	4	oligonucleotides	oligonucleotide	NNS
cord-328460-thx9zh11	164	5	with	with	IN
cord-328460-thx9zh11	164	6	the	the	DT
cord-328460-thx9zh11	164	7	two	two	CD
cord-328460-thx9zh11	164	8	lateral	lateral	JJ
cord-328460-thx9zh11	164	9	sequences	sequence	NNS
cord-328460-thx9zh11	164	10	complementary	complementary	JJ
cord-328460-thx9zh11	164	11	to	to	IN
cord-328460-thx9zh11	164	12	two	two	CD
cord-328460-thx9zh11	164	13	target	target	NN
cord-328460-thx9zh11	164	14	sequences	sequence	NNS
cord-328460-thx9zh11	164	15	that	that	WDT
cord-328460-thx9zh11	164	16	are	be	VBP
cord-328460-thx9zh11	164	17	connected	connect	VBN
cord-328460-thx9zh11	164	18	by	by	IN
cord-328460-thx9zh11	164	19	a	a	DT
cord-328460-thx9zh11	164	20	linker	linker	NN
cord-328460-thx9zh11	164	21	sequence	sequence	NN
cord-328460-thx9zh11	164	22	.	.	.
cord-328460-thx9zh11	165	1	When	when	WRB
cord-328460-thx9zh11	165	2	the	the	DT
cord-328460-thx9zh11	165	3	padlock	padlock	NN
cord-328460-thx9zh11	165	4	probe	probe	NN
cord-328460-thx9zh11	165	5	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	165	6	the	the	DT
cord-328460-thx9zh11	165	7	target	target	NN
cord-328460-thx9zh11	165	8	sequences	sequence	NNS
cord-328460-thx9zh11	165	9	it	-PRON-	PRP
cord-328460-thx9zh11	165	10	circularizes	circularize	VBZ
cord-328460-thx9zh11	165	11	.	.	.
cord-328460-thx9zh11	166	1	Upon	upon	IN
cord-328460-thx9zh11	166	2	recognition	recognition	NN
cord-328460-thx9zh11	166	3	of	of	IN
cord-328460-thx9zh11	166	4	the	the	DT
cord-328460-thx9zh11	166	5	target	target	NN
cord-328460-thx9zh11	166	6	,	,	,
cord-328460-thx9zh11	166	7	the	the	DT
cord-328460-thx9zh11	166	8	padlock	padlock	NN
cord-328460-thx9zh11	166	9	segments	segment	NNS
cord-328460-thx9zh11	166	10	are	be	VBP
cord-328460-thx9zh11	166	11	sealed	seal	VBN
cord-328460-thx9zh11	166	12	through	through	IN
cord-328460-thx9zh11	166	13	the	the	DT
cord-328460-thx9zh11	166	14	action	action	NN
cord-328460-thx9zh11	166	15	of	of	IN
cord-328460-thx9zh11	166	16	a	a	DT
cord-328460-thx9zh11	166	17	DNA	dna	NN
cord-328460-thx9zh11	166	18	ligase	ligase	NN
cord-328460-thx9zh11	166	19	alone	alone	RB
cord-328460-thx9zh11	166	20	or	or	CC
cord-328460-thx9zh11	166	21	in	in	IN
cord-328460-thx9zh11	166	22	combination	combination	NN
cord-328460-thx9zh11	166	23	with	with	IN
cord-328460-thx9zh11	166	24	a	a	DT
cord-328460-thx9zh11	166	25	DNA	dna	NN
cord-328460-thx9zh11	166	26	polymerase	polymerase	NN
cord-328460-thx9zh11	166	27	[	[	-LRB-
cord-328460-thx9zh11	166	28	82	82	CD
cord-328460-thx9zh11	166	29	]	]	-RRB-
cord-328460-thx9zh11	166	30	.	.	.
cord-328460-thx9zh11	167	1	As	as	IN
cord-328460-thx9zh11	167	2	the	the	DT
cord-328460-thx9zh11	167	3	circularizing	circularize	VBG
cord-328460-thx9zh11	167	4	step	step	NN
cord-328460-thx9zh11	167	5	of	of	IN
cord-328460-thx9zh11	167	6	a	a	DT
cord-328460-thx9zh11	167	7	padlock	padlock	NN
cord-328460-thx9zh11	167	8	probe	probe	NN
cord-328460-thx9zh11	167	9	is	be	VBZ
cord-328460-thx9zh11	167	10	strictly	strictly	RB
cord-328460-thx9zh11	167	11	target	target	NN
cord-328460-thx9zh11	167	12	-	-	HYPH
cord-328460-thx9zh11	167	13	dependent	dependent	JJ
cord-328460-thx9zh11	167	14	,	,	,
cord-328460-thx9zh11	167	15	high	high	JJ
cord-328460-thx9zh11	167	16	enough	enough	JJ
cord-328460-thx9zh11	167	17	specificity	specificity	NN
cord-328460-thx9zh11	167	18	is	be	VBZ
cord-328460-thx9zh11	167	19	ensured	ensure	VBN
cord-328460-thx9zh11	167	20	to	to	TO
cord-328460-thx9zh11	167	21	allow	allow	VB
cord-328460-thx9zh11	167	22	SNP	SNP	NNP
cord-328460-thx9zh11	167	23	analysis	analysis	NN
cord-328460-thx9zh11	167	24	.	.	.
cord-328460-thx9zh11	168	1	Sample	sample	JJ
cord-328460-thx9zh11	168	2	pre	pre	NNS
cord-328460-thx9zh11	168	3	-	-	NNS
cord-328460-thx9zh11	168	4	treatments	treatment	NNS
cord-328460-thx9zh11	168	5	are	be	VBP
cord-328460-thx9zh11	168	6	required	require	VBN
cord-328460-thx9zh11	168	7	before	before	IN
cord-328460-thx9zh11	168	8	the	the	DT
cord-328460-thx9zh11	168	9	padlock	padlock	NN
cord-328460-thx9zh11	168	10	probe	probe	NN
cord-328460-thx9zh11	168	11	circularization	circularization	VBP
cord-328460-thx9zh11	168	12	in	in	IN
cord-328460-thx9zh11	168	13	order	order	NN
cord-328460-thx9zh11	168	14	to	to	TO
cord-328460-thx9zh11	168	15	allow	allow	VB
cord-328460-thx9zh11	168	16	the	the	DT
cord-328460-thx9zh11	168	17	transformation	transformation	NN
cord-328460-thx9zh11	168	18	of	of	IN
cord-328460-thx9zh11	168	19	duplex	duplex	JJ
cord-328460-thx9zh11	168	20	genomic	genomic	JJ
cord-328460-thx9zh11	168	21	DNA	dna	NN
cord-328460-thx9zh11	168	22	into	into	IN
cord-328460-thx9zh11	168	23	accessible	accessible	JJ
cord-328460-thx9zh11	168	24	single	single	RB
cord-328460-thx9zh11	168	25	-	-	HYPH
cord-328460-thx9zh11	168	26	stranded	strand	VBN
cord-328460-thx9zh11	168	27	DNA	dna	NN
cord-328460-thx9zh11	168	28	targets	target	NNS
cord-328460-thx9zh11	168	29	.	.	.
cord-328460-thx9zh11	169	1	For	for	IN
cord-328460-thx9zh11	169	2	this	this	DT
cord-328460-thx9zh11	169	3	reason	reason	NN
cord-328460-thx9zh11	169	4	genomic	genomic	JJ
cord-328460-thx9zh11	169	5	DNA	dna	NN
cord-328460-thx9zh11	169	6	is	be	VBZ
cord-328460-thx9zh11	169	7	first	first	RB
cord-328460-thx9zh11	169	8	digested	digest	VBN
cord-328460-thx9zh11	169	9	with	with	IN
cord-328460-thx9zh11	169	10	restriction	restriction	NN
cord-328460-thx9zh11	169	11	enzymes	enzyme	NNS
cord-328460-thx9zh11	169	12	that	that	WDT
cord-328460-thx9zh11	169	13	produce	produce	VBP
cord-328460-thx9zh11	169	14	small	small	JJ
cord-328460-thx9zh11	169	15	sized	sized	JJ
cord-328460-thx9zh11	169	16	dsDNA	dsdna	RB
cord-328460-thx9zh11	169	17	,	,	,
cord-328460-thx9zh11	169	18	then	then	RB
cord-328460-thx9zh11	169	19	subjected	subject	VBN
cord-328460-thx9zh11	169	20	to	to	IN
cord-328460-thx9zh11	169	21	degradation	degradation	NN
cord-328460-thx9zh11	169	22	by	by	IN
cord-328460-thx9zh11	169	23	5'-3	5'-3	CD
cord-328460-thx9zh11	169	24	'	'	''
cord-328460-thx9zh11	169	25	exonucleolysis	exonucleolysis	NN
cord-328460-thx9zh11	169	26	.	.	.
cord-328460-thx9zh11	170	1	Even	even	RB
cord-328460-thx9zh11	170	2	mRNA	mrna	NN
cord-328460-thx9zh11	170	3	can	can	MD
cord-328460-thx9zh11	170	4	be	be	VB
cord-328460-thx9zh11	170	5	amplified	amplify	VBN
cord-328460-thx9zh11	170	6	through	through	IN
cord-328460-thx9zh11	170	7	the	the	DT
cord-328460-thx9zh11	170	8	RCA	rca	NN
cord-328460-thx9zh11	170	9	approach	approach	NN
cord-328460-thx9zh11	170	10	but	but	CC
cord-328460-thx9zh11	170	11	,	,	,
cord-328460-thx9zh11	170	12	in	in	IN
cord-328460-thx9zh11	170	13	this	this	DT
cord-328460-thx9zh11	170	14	case	case	NN
cord-328460-thx9zh11	170	15	,	,	,
cord-328460-thx9zh11	170	16	an	an	DT
cord-328460-thx9zh11	170	17	mRNA	mrna	NN
cord-328460-thx9zh11	170	18	pre	pre	JJ
cord-328460-thx9zh11	170	19	-	-	JJ
cord-328460-thx9zh11	170	20	treatment	treatment	NN
cord-328460-thx9zh11	170	21	is	be	VBZ
cord-328460-thx9zh11	170	22	performed	perform	VBN
cord-328460-thx9zh11	170	23	which	which	WDT
cord-328460-thx9zh11	170	24	consists	consist	VBZ
cord-328460-thx9zh11	170	25	of	of	IN
cord-328460-thx9zh11	170	26	cDNA	cdna	NN
cord-328460-thx9zh11	170	27	synthesis	synthesis	NN
cord-328460-thx9zh11	170	28	by	by	IN
cord-328460-thx9zh11	170	29	a	a	DT
cord-328460-thx9zh11	170	30	reverse	reverse	JJ
cord-328460-thx9zh11	170	31	-	-	HYPH
cord-328460-thx9zh11	170	32	transcription	transcription	NN
cord-328460-thx9zh11	170	33	followed	follow	VBN
cord-328460-thx9zh11	170	34	by	by	IN
cord-328460-thx9zh11	170	35	mRNA	mrna	NN
cord-328460-thx9zh11	170	36	degradation	degradation	NN
cord-328460-thx9zh11	170	37	by	by	IN
cord-328460-thx9zh11	170	38	RNase	rnase	NN
cord-328460-thx9zh11	170	39	H	h	NN
cord-328460-thx9zh11	170	40	digestion	digestion	NN
cord-328460-thx9zh11	170	41	[	[	-LRB-
cord-328460-thx9zh11	170	42	10	10	CD
cord-328460-thx9zh11	170	43	]	]	-RRB-
cord-328460-thx9zh11	170	44	.	.	.
cord-328460-thx9zh11	171	1	In	in	IN
cord-328460-thx9zh11	171	2	both	both	DT
cord-328460-thx9zh11	171	3	cases	case	NNS
cord-328460-thx9zh11	171	4	,	,	,
cord-328460-thx9zh11	171	5	RCA	RCA	NNP
cord-328460-thx9zh11	171	6	of	of	IN
cord-328460-thx9zh11	171	7	padlock	padlock	NN
cord-328460-thx9zh11	171	8	probes	probe	NNS
cord-328460-thx9zh11	171	9	produces	produce	VBZ
cord-328460-thx9zh11	171	10	a	a	DT
cord-328460-thx9zh11	171	11	large	large	JJ
cord-328460-thx9zh11	171	12	DNA	dna	NN
cord-328460-thx9zh11	171	13	product	product	NN
cord-328460-thx9zh11	171	14	that	that	WDT
cord-328460-thx9zh11	171	15	collapses	collapse	VBZ
cord-328460-thx9zh11	171	16	into	into	IN
cord-328460-thx9zh11	171	17	a	a	DT
cord-328460-thx9zh11	171	18	submicrometer	submicrometer	NN
cord-328460-thx9zh11	171	19	-	-	HYPH
cord-328460-thx9zh11	171	20	sized	sized	JJ
cord-328460-thx9zh11	171	21	concatamer	concatamer	NN
cord-328460-thx9zh11	171	22	that	that	WDT
cord-328460-thx9zh11	171	23	can	can	MD
cord-328460-thx9zh11	171	24	be	be	VB
cord-328460-thx9zh11	171	25	tagged	tag	VBN
cord-328460-thx9zh11	171	26	and	and	CC
cord-328460-thx9zh11	171	27	analyzed	analyze	VBN
cord-328460-thx9zh11	171	28	for	for	IN
cord-328460-thx9zh11	171	29	single	single	JJ
cord-328460-thx9zh11	171	30	molecule	molecule	NN
cord-328460-thx9zh11	171	31	detection	detection	NN
cord-328460-thx9zh11	171	32	[	[	-LRB-
cord-328460-thx9zh11	171	33	83	83	CD
cord-328460-thx9zh11	171	34	]	]	-RRB-
cord-328460-thx9zh11	171	35	.	.	.
cord-328460-thx9zh11	172	1	The	the	DT
cord-328460-thx9zh11	172	2	creation	creation	NN
cord-328460-thx9zh11	172	3	of	of	IN
cord-328460-thx9zh11	172	4	one	one	CD
cord-328460-thx9zh11	172	5	rolling	roll	VBG
cord-328460-thx9zh11	172	6	circle	circle	NN
cord-328460-thx9zh11	172	7	product	product	NN
cord-328460-thx9zh11	172	8	(	(	-LRB-
cord-328460-thx9zh11	172	9	RCP	RCP	NNP
cord-328460-thx9zh11	172	10	)	)	-RRB-
cord-328460-thx9zh11	172	11	for	for	IN
cord-328460-thx9zh11	172	12	each	each	DT
cord-328460-thx9zh11	172	13	recognized	recognize	VBN
cord-328460-thx9zh11	172	14	target	target	NN
cord-328460-thx9zh11	172	15	makes	make	VBZ
cord-328460-thx9zh11	172	16	optical	optical	JJ
cord-328460-thx9zh11	172	17	detection	detection	NN
cord-328460-thx9zh11	172	18	and	and	CC
cord-328460-thx9zh11	172	19	quantification	quantification	NN
cord-328460-thx9zh11	172	20	possible	possible	JJ
cord-328460-thx9zh11	172	21	by	by	IN
cord-328460-thx9zh11	172	22	using	use	VBG
cord-328460-thx9zh11	172	23	fluorescence	fluorescence	NN
cord-328460-thx9zh11	172	24	optical	optical	JJ
cord-328460-thx9zh11	172	25	microscopy	microscopy	NNS
cord-328460-thx9zh11	172	26	[	[	-LRB-
cord-328460-thx9zh11	172	27	84	84	CD
cord-328460-thx9zh11	172	28	]	]	-RRB-
cord-328460-thx9zh11	172	29	.	.	.
cord-328460-thx9zh11	173	1	Although	although	IN
cord-328460-thx9zh11	173	2	small	small	JJ
cord-328460-thx9zh11	173	3	circular	circular	JJ
cord-328460-thx9zh11	173	4	DNA	dna	NN
cord-328460-thx9zh11	173	5	molecules	molecule	NNS
cord-328460-thx9zh11	173	6	are	be	VBP
cord-328460-thx9zh11	173	7	uncommon	uncommon	JJ
cord-328460-thx9zh11	173	8	in	in	IN
cord-328460-thx9zh11	173	9	higher	high	JJR
cord-328460-thx9zh11	173	10	organisms	organism	NNS
cord-328460-thx9zh11	173	11	,	,	,
cord-328460-thx9zh11	173	12	they	-PRON-	PRP
cord-328460-thx9zh11	173	13	are	be	VBP
cord-328460-thx9zh11	173	14	widespread	widespread	JJ
cord-328460-thx9zh11	173	15	in	in	IN
cord-328460-thx9zh11	173	16	several	several	JJ
cord-328460-thx9zh11	173	17	microorganisms	microorganism	NNS
cord-328460-thx9zh11	173	18	such	such	JJ
cord-328460-thx9zh11	173	19	as	as	IN
cord-328460-thx9zh11	173	20	viruses	virus	NNS
cord-328460-thx9zh11	173	21	,	,	,
cord-328460-thx9zh11	173	22	bacteriophages	bacteriophage	NNS
cord-328460-thx9zh11	173	23	and	and	CC
cord-328460-thx9zh11	173	24	bacteria	bacteria	NNS
cord-328460-thx9zh11	173	25	.	.	.
cord-328460-thx9zh11	174	1	RCA	RCA	NNP
cord-328460-thx9zh11	174	2	has	have	VBZ
cord-328460-thx9zh11	174	3	also	also	RB
cord-328460-thx9zh11	174	4	been	be	VBN
cord-328460-thx9zh11	174	5	used	use	VBN
cord-328460-thx9zh11	174	6	to	to	TO
cord-328460-thx9zh11	174	7	amplify	amplify	VB
cord-328460-thx9zh11	174	8	circular	circular	JJ
cord-328460-thx9zh11	174	9	genomes	genome	NNS
cord-328460-thx9zh11	174	10	of	of	IN
cord-328460-thx9zh11	174	11	DNA	dna	NN
cord-328460-thx9zh11	174	12	viruses	virus	NNS
cord-328460-thx9zh11	174	13	,	,	,
cord-328460-thx9zh11	174	14	to	to	TO
cord-328460-thx9zh11	174	15	identify	identify	VB
cord-328460-thx9zh11	174	16	phage	phage	NN
cord-328460-thx9zh11	174	17	genomes	genome	NNS
cord-328460-thx9zh11	174	18	carrying	carry	VBG
cord-328460-thx9zh11	174	19	lethal	lethal	JJ
cord-328460-thx9zh11	174	20	mutations	mutation	NNS
cord-328460-thx9zh11	174	21	and	and	CC
cord-328460-thx9zh11	174	22	bacteria	bacteria	NNS
cord-328460-thx9zh11	174	23	by	by	IN
cord-328460-thx9zh11	174	24	using	use	VBG
cord-328460-thx9zh11	174	25	primers	primer	NNS
cord-328460-thx9zh11	174	26	that	that	WDT
cord-328460-thx9zh11	174	27	include	include	VBP
cord-328460-thx9zh11	174	28	the	the	DT
cord-328460-thx9zh11	174	29	sequence	sequence	NN
cord-328460-thx9zh11	174	30	of	of	IN
cord-328460-thx9zh11	174	31	the	the	DT
cord-328460-thx9zh11	174	32	target	target	NN
cord-328460-thx9zh11	174	33	DNA	dna	NN
cord-328460-thx9zh11	174	34	[	[	-LRB-
cord-328460-thx9zh11	174	35	85	85	CD
cord-328460-thx9zh11	174	36	,	,	,
cord-328460-thx9zh11	174	37	86	86	CD
cord-328460-thx9zh11	174	38	]	]	-RRB-
cord-328460-thx9zh11	174	39	.	.	.
cord-328460-thx9zh11	175	1	The	the	DT
cord-328460-thx9zh11	175	2	method	method	NN
cord-328460-thx9zh11	175	3	is	be	VBZ
cord-328460-thx9zh11	175	4	called	call	VBN
cord-328460-thx9zh11	175	5	multiply	multiply	RB
cord-328460-thx9zh11	175	6	primed	prime	VBN
cord-328460-thx9zh11	175	7	RCA	RCA	NNP
cord-328460-thx9zh11	175	8	and	and	CC
cord-328460-thx9zh11	175	9	uses	use	VBZ
cord-328460-thx9zh11	175	10	φ29	φ29	NN
cord-328460-thx9zh11	175	11	DNA	dna	NN
cord-328460-thx9zh11	175	12	polymerase	polymerase	NN
cord-328460-thx9zh11	175	13	and	and	CC
cord-328460-thx9zh11	175	14	an	an	DT
cord-328460-thx9zh11	175	15	exonuclease	exonuclease	NN
cord-328460-thx9zh11	175	16	-	-	HYPH
cord-328460-thx9zh11	175	17	resistant	resistant	JJ
cord-328460-thx9zh11	175	18	random	random	JJ
cord-328460-thx9zh11	175	19	primer	primer	NN
cord-328460-thx9zh11	175	20	.	.	.
cord-328460-thx9zh11	176	1	Mahmoudian	Mahmoudian	NNP
cord-328460-thx9zh11	176	2	et	et	NNP
cord-328460-thx9zh11	176	3	al	al	NNP
cord-328460-thx9zh11	176	4	.	.	NNP
cord-328460-thx9zh11	176	5	introduced	introduce	VBD
cord-328460-thx9zh11	176	6	an	an	DT
cord-328460-thx9zh11	176	7	integrated	integrate	VBN
cord-328460-thx9zh11	176	8	platform	platform	NN
cord-328460-thx9zh11	176	9	for	for	IN
cord-328460-thx9zh11	176	10	RCA	RCA	NNP
cord-328460-thx9zh11	176	11	and	and	CC
cord-328460-thx9zh11	176	12	circle	circle	NN
cord-328460-thx9zh11	176	13	-	-	HYPH
cord-328460-thx9zh11	176	14	to	to	IN
cord-328460-thx9zh11	176	15	-	-	HYPH
cord-328460-thx9zh11	176	16	circle	circle	NN
cord-328460-thx9zh11	176	17	amplification	amplification	NN
cord-328460-thx9zh11	176	18	(	(	-LRB-
cord-328460-thx9zh11	176	19	C2CA	C2CA	NNP
cord-328460-thx9zh11	176	20	)	)	-RRB-
cord-328460-thx9zh11	176	21	of	of	IN
cord-328460-thx9zh11	176	22	padlock	padlock	NN
cord-328460-thx9zh11	176	23	probes	probe	NNS
cord-328460-thx9zh11	176	24	on	on	IN
cord-328460-thx9zh11	176	25	a	a	DT
cord-328460-thx9zh11	176	26	poly(methyl	poly(methyl	JJ
cord-328460-thx9zh11	176	27	methacrylate	methacrylate	NN
cord-328460-thx9zh11	176	28	)	)	-RRB-
cord-328460-thx9zh11	177	1	(	(	-LRB-
cord-328460-thx9zh11	177	2	PMMA	PMMA	NNP
cord-328460-thx9zh11	177	3	)	)	-RRB-
cord-328460-thx9zh11	177	4	microchip	microchip	NN
cord-328460-thx9zh11	177	5	[	[	-LRB-
cord-328460-thx9zh11	177	6	87	87	CD
cord-328460-thx9zh11	177	7	]	]	-RRB-
cord-328460-thx9zh11	177	8	.	.	.
cord-328460-thx9zh11	178	1	C2CA	C2CA	NNP
cord-328460-thx9zh11	178	2	is	be	VBZ
cord-328460-thx9zh11	178	3	an	an	DT
cord-328460-thx9zh11	178	4	RCA	RCA	NNP
cord-328460-thx9zh11	178	5	-	-	HYPH
cord-328460-thx9zh11	178	6	based	base	VBN
cord-328460-thx9zh11	178	7	amplification	amplification	NN
cord-328460-thx9zh11	178	8	method	method	NN
cord-328460-thx9zh11	178	9	for	for	IN
cord-328460-thx9zh11	178	10	small	small	JJ
cord-328460-thx9zh11	178	11	DNA	dna	NN
cord-328460-thx9zh11	178	12	circles	circle	NNS
cord-328460-thx9zh11	178	13	that	that	WDT
cord-328460-thx9zh11	178	14	produces	produce	VBZ
cord-328460-thx9zh11	178	15	single	single	JJ
cord-328460-thx9zh11	178	16	stable	stable	JJ
cord-328460-thx9zh11	178	17	dsDNA	dsdna	JJ
cord-328460-thx9zh11	178	18	fragments	fragment	NNS
cord-328460-thx9zh11	178	19	trough	trough	IN
cord-328460-thx9zh11	178	20	the	the	DT
cord-328460-thx9zh11	178	21	repetition	repetition	NN
cord-328460-thx9zh11	178	22	of	of	IN
cord-328460-thx9zh11	178	23	a	a	DT
cord-328460-thx9zh11	178	24	few	few	JJ
cord-328460-thx9zh11	178	25	(	(	-LRB-
cord-328460-thx9zh11	178	26	up	up	IN
cord-328460-thx9zh11	178	27	to	to	TO
cord-328460-thx9zh11	178	28	three	three	CD
cord-328460-thx9zh11	178	29	)	)	-RRB-
cord-328460-thx9zh11	178	30	cycles	cycle	NNS
cord-328460-thx9zh11	178	31	of	of	IN
cord-328460-thx9zh11	178	32	replication	replication	NN
cord-328460-thx9zh11	178	33	,	,	,
cord-328460-thx9zh11	178	34	monomerization	monomerization	NN
cord-328460-thx9zh11	178	35	,	,	,
cord-328460-thx9zh11	178	36	and	and	CC
cord-328460-thx9zh11	178	37	ligation	ligation	NN
cord-328460-thx9zh11	178	38	steps	step	NNS
cord-328460-thx9zh11	178	39	[	[	-LRB-
cord-328460-thx9zh11	178	40	88	88	CD
cord-328460-thx9zh11	178	41	]	]	-RRB-
cord-328460-thx9zh11	178	42	.	.	.
cord-328460-thx9zh11	179	1	After	after	IN
cord-328460-thx9zh11	179	2	each	each	DT
cord-328460-thx9zh11	179	3	cycle	cycle	NN
cord-328460-thx9zh11	179	4	of	of	IN
cord-328460-thx9zh11	179	5	C2CA	C2CA	NNP
cord-328460-thx9zh11	179	6	,	,	,
cord-328460-thx9zh11	179	7	RCA	RCA	NNP
cord-328460-thx9zh11	179	8	products	product	NNS
cord-328460-thx9zh11	179	9	generated	generate	VBN
cord-328460-thx9zh11	179	10	in	in	IN
cord-328460-thx9zh11	179	11	the	the	DT
cord-328460-thx9zh11	179	12	replication	replication	NN
cord-328460-thx9zh11	179	13	step	step	NN
cord-328460-thx9zh11	179	14	are	be	VBP
cord-328460-thx9zh11	179	15	subjected	subject	VBN
cord-328460-thx9zh11	179	16	to	to	IN
cord-328460-thx9zh11	179	17	restriction	restriction	NN
cord-328460-thx9zh11	179	18	digestion	digestion	NN
cord-328460-thx9zh11	179	19	to	to	TO
cord-328460-thx9zh11	179	20	be	be	VB
cord-328460-thx9zh11	179	21	cut	cut	VBN
cord-328460-thx9zh11	179	22	into	into	IN
cord-328460-thx9zh11	179	23	linear	linear	JJ
cord-328460-thx9zh11	179	24	monomer	monomer	NN
cord-328460-thx9zh11	179	25	and	and	CC
cord-328460-thx9zh11	179	26	then	then	RB
cord-328460-thx9zh11	179	27	subjected	subject	VBN
cord-328460-thx9zh11	179	28	to	to	IN
cord-328460-thx9zh11	179	29	ligation	ligation	NN
cord-328460-thx9zh11	179	30	that	that	WDT
cord-328460-thx9zh11	179	31	produces	produce	VBZ
cord-328460-thx9zh11	179	32	circular	circular	JJ
cord-328460-thx9zh11	179	33	ssDNA	ssdna	JJ
cord-328460-thx9zh11	179	34	monomers	monomer	NNS
cord-328460-thx9zh11	179	35	.	.	.
cord-328460-thx9zh11	180	1	The	the	DT
cord-328460-thx9zh11	180	2	introduction	introduction	NN
cord-328460-thx9zh11	180	3	of	of	IN
cord-328460-thx9zh11	180	4	an	an	DT
cord-328460-thx9zh11	180	5	alternative	alternative	JJ
cord-328460-thx9zh11	180	6	step	step	NN
cord-328460-thx9zh11	180	7	for	for	IN
cord-328460-thx9zh11	180	8	ligation	ligation	NN
cord-328460-thx9zh11	180	9	,	,	,
cord-328460-thx9zh11	180	10	termed	term	VBN
cord-328460-thx9zh11	180	11	"	"	``
cord-328460-thx9zh11	180	12	duplication	duplication	NN
cord-328460-thx9zh11	180	13	"	"	''
cord-328460-thx9zh11	180	14	,	,	,
cord-328460-thx9zh11	180	15	enables	enable	VBZ
cord-328460-thx9zh11	180	16	the	the	DT
cord-328460-thx9zh11	180	17	conversion	conversion	NN
cord-328460-thx9zh11	180	18	of	of	IN
cord-328460-thx9zh11	180	19	ssDNA	ssdna	NN
cord-328460-thx9zh11	180	20	to	to	IN
cord-328460-thx9zh11	180	21	dsDNA	dsdna	RB
cord-328460-thx9zh11	180	22	product	product	VB
cord-328460-thx9zh11	180	23	[	[	-LRB-
cord-328460-thx9zh11	180	24	89	89	CD
cord-328460-thx9zh11	180	25	]	]	-RRB-
cord-328460-thx9zh11	180	26	.	.	.
cord-328460-thx9zh11	181	1	In	in	IN
cord-328460-thx9zh11	181	2	the	the	DT
cord-328460-thx9zh11	181	3	latter	latter	JJ
cord-328460-thx9zh11	181	4	case	case	NN
cord-328460-thx9zh11	181	5	,	,	,
cord-328460-thx9zh11	181	6	RCA	RCA	NNP
cord-328460-thx9zh11	181	7	and	and	CC
cord-328460-thx9zh11	181	8	C2CA	C2CA	NNP
cord-328460-thx9zh11	181	9	were	be	VBD
cord-328460-thx9zh11	181	10	successfully	successfully	RB
cord-328460-thx9zh11	181	11	carried	carry	VBN
cord-328460-thx9zh11	181	12	out	out	RP
cord-328460-thx9zh11	181	13	in	in	IN
cord-328460-thx9zh11	181	14	a	a	DT
cord-328460-thx9zh11	181	15	microchip	microchip	NN
cord-328460-thx9zh11	181	16	and	and	CC
cord-328460-thx9zh11	181	17	the	the	DT
cord-328460-thx9zh11	181	18	products	product	NNS
cord-328460-thx9zh11	181	19	were	be	VBD
cord-328460-thx9zh11	181	20	subsequently	subsequently	RB
cord-328460-thx9zh11	181	21	detected	detect	VBN
cord-328460-thx9zh11	181	22	on	on	IN
cord-328460-thx9zh11	181	23	the	the	DT
cord-328460-thx9zh11	181	24	same	same	JJ
cord-328460-thx9zh11	181	25	channel	channel	NN
cord-328460-thx9zh11	181	26	of	of	IN
cord-328460-thx9zh11	181	27	the	the	DT
cord-328460-thx9zh11	181	28	microchip	microchip	NN
cord-328460-thx9zh11	181	29	by	by	IN
cord-328460-thx9zh11	181	30	micro	micro	JJ
cord-328460-thx9zh11	181	31	-	-	NN
cord-328460-thx9zh11	181	32	electrophoresis	electrophoresis	JJ
cord-328460-thx9zh11	181	33	(	(	-LRB-
cord-328460-thx9zh11	181	34	µ-CE	µ-CE	NNP
cord-328460-thx9zh11	181	35	)	)	-RRB-
cord-328460-thx9zh11	181	36	.	.	.
cord-328460-thx9zh11	182	1	One	one	CD
cord-328460-thx9zh11	182	2	of	of	IN
cord-328460-thx9zh11	182	3	the	the	DT
cord-328460-thx9zh11	182	4	major	major	JJ
cord-328460-thx9zh11	182	5	advantages	advantage	NNS
cord-328460-thx9zh11	182	6	of	of	IN
cord-328460-thx9zh11	182	7	this	this	DT
cord-328460-thx9zh11	182	8	integrated	integrated	JJ
cord-328460-thx9zh11	182	9	platform	platform	NN
cord-328460-thx9zh11	182	10	is	be	VBZ
cord-328460-thx9zh11	182	11	that	that	IN
cord-328460-thx9zh11	182	12	it	-PRON-	PRP
cord-328460-thx9zh11	182	13	combines	combine	VBZ
cord-328460-thx9zh11	182	14	the	the	DT
cord-328460-thx9zh11	182	15	simple	simple	JJ
cord-328460-thx9zh11	182	16	RCA	RCA	NNP
cord-328460-thx9zh11	182	17	temperature	temperature	NN
cord-328460-thx9zh11	182	18	control	control	NN
cord-328460-thx9zh11	182	19	(	(	-LRB-
cord-328460-thx9zh11	182	20	stable	stable	JJ
cord-328460-thx9zh11	182	21	temperature	temperature	NN
cord-328460-thx9zh11	182	22	at	at	IN
cord-328460-thx9zh11	182	23	37	37	CD
cord-328460-thx9zh11	182	24	°	°	NN
cord-328460-thx9zh11	182	25	C	c	NN
cord-328460-thx9zh11	182	26	)	)	-RRB-
cord-328460-thx9zh11	182	27	with	with	IN
cord-328460-thx9zh11	182	28	versatility	versatility	NN
cord-328460-thx9zh11	182	29	,	,	,
cord-328460-thx9zh11	182	30	high	high	JJ
cord-328460-thx9zh11	182	31	speed	speed	NN
cord-328460-thx9zh11	182	32	,	,	,
cord-328460-thx9zh11	182	33	easy	easy	JJ
cord-328460-thx9zh11	182	34	automation	automation	NN
cord-328460-thx9zh11	182	35	,	,	,
cord-328460-thx9zh11	182	36	low	low	JJ
cord-328460-thx9zh11	182	37	cost	cost	NN
cord-328460-thx9zh11	182	38	and	and	CC
cord-328460-thx9zh11	182	39	simplicity	simplicity	NN
cord-328460-thx9zh11	182	40	of	of	IN
cord-328460-thx9zh11	182	41	µ-CE	µ-CE	NNP
cord-328460-thx9zh11	182	42	,	,	,
cord-328460-thx9zh11	182	43	providing	provide	VBG
cord-328460-thx9zh11	182	44	a	a	DT
cord-328460-thx9zh11	182	45	reliable	reliable	JJ
cord-328460-thx9zh11	182	46	and	and	CC
cord-328460-thx9zh11	182	47	fast	fast	JJ
cord-328460-thx9zh11	182	48	technique	technique	NN
cord-328460-thx9zh11	182	49	for	for	IN
cord-328460-thx9zh11	182	50	SNPs	SNPs	NNP
cord-328460-thx9zh11	182	51	analyses	analysis	NNS
cord-328460-thx9zh11	182	52	.	.	.
cord-328460-thx9zh11	183	1	A	a	DT
cord-328460-thx9zh11	183	2	droplet	droplet	NN
cord-328460-thx9zh11	183	3	of	of	IN
cord-328460-thx9zh11	183	4	microfluidics	microfluidics	NN
cord-328460-thx9zh11	183	5	-	-	HYPH
cord-328460-thx9zh11	183	6	based	base	VBN
cord-328460-thx9zh11	183	7	hyperbranched	hyperbranched	NNP
cord-328460-thx9zh11	183	8	rolling	roll	VBG
cord-328460-thx9zh11	183	9	circle	circle	NN
cord-328460-thx9zh11	183	10	amplification	amplification	NN
cord-328460-thx9zh11	183	11	(	(	-LRB-
cord-328460-thx9zh11	183	12	HRCA	HRCA	NNP
cord-328460-thx9zh11	183	13	)	)	-RRB-
cord-328460-thx9zh11	183	14	for	for	IN
cord-328460-thx9zh11	183	15	single	single	JJ
cord-328460-thx9zh11	183	16	-	-	HYPH
cord-328460-thx9zh11	183	17	molecule	molecule	NN
cord-328460-thx9zh11	183	18	DNA	dna	NN
cord-328460-thx9zh11	183	19	amplification	amplification	NN
cord-328460-thx9zh11	183	20	and	and	CC
cord-328460-thx9zh11	183	21	analysis	analysis	NN
cord-328460-thx9zh11	183	22	has	have	VBZ
cord-328460-thx9zh11	183	23	been	be	VBN
cord-328460-thx9zh11	183	24	also	also	RB
cord-328460-thx9zh11	183	25	described	describe	VBN
cord-328460-thx9zh11	183	26	[	[	-LRB-
cord-328460-thx9zh11	183	27	90	90	CD
cord-328460-thx9zh11	183	28	]	]	-RRB-
cord-328460-thx9zh11	183	29	.	.	.
cord-328460-thx9zh11	184	1	The	the	DT
cord-328460-thx9zh11	184	2	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	184	3	devices	device	NNS
cord-328460-thx9zh11	184	4	included	include	VBD
cord-328460-thx9zh11	184	5	separate	separate	JJ
cord-328460-thx9zh11	184	6	devices	device	NNS
cord-328460-thx9zh11	184	7	for	for	IN
cord-328460-thx9zh11	184	8	droplet	droplet	NN
cord-328460-thx9zh11	184	9	generation	generation	NN
cord-328460-thx9zh11	184	10	,	,	,
cord-328460-thx9zh11	184	11	fusion	fusion	NN
cord-328460-thx9zh11	184	12	and	and	CC
cord-328460-thx9zh11	184	13	detection	detection	NN
cord-328460-thx9zh11	184	14	and	and	CC
cord-328460-thx9zh11	184	15	allowed	allow	VBD
cord-328460-thx9zh11	184	16	the	the	DT
cord-328460-thx9zh11	184	17	amplified	amplify	VBN
cord-328460-thx9zh11	184	18	DNA	dna	NN
cord-328460-thx9zh11	184	19	in	in	IN
cord-328460-thx9zh11	184	20	each	each	DT
cord-328460-thx9zh11	184	21	of	of	IN
cord-328460-thx9zh11	184	22	the	the	DT
cord-328460-thx9zh11	184	23	2	2	CD
cord-328460-thx9zh11	184	24	pL	pl	NN
cord-328460-thx9zh11	184	25	droplets	droplet	NNS
cord-328460-thx9zh11	184	26	to	to	TO
cord-328460-thx9zh11	184	27	be	be	VB
cord-328460-thx9zh11	184	28	analyzed	analyze	VBN
cord-328460-thx9zh11	184	29	by	by	IN
cord-328460-thx9zh11	184	30	measuring	measure	VBG
cord-328460-thx9zh11	184	31	the	the	DT
cord-328460-thx9zh11	184	32	activity	activity	NN
cord-328460-thx9zh11	184	33	of	of	IN
cord-328460-thx9zh11	184	34	the	the	DT
cord-328460-thx9zh11	184	35	encoded	encode	VBN
cord-328460-thx9zh11	184	36	enzyme	enzyme	NN
cord-328460-thx9zh11	184	37	(	(	-LRB-
cord-328460-thx9zh11	184	38	β	β	NN
cord-328460-thx9zh11	184	39	-	-	HYPH
cord-328460-thx9zh11	184	40	galactosidase	galactosidase	NN
cord-328460-thx9zh11	184	41	)	)	-RRB-
cord-328460-thx9zh11	184	42	after	after	IN
cord-328460-thx9zh11	184	43	the	the	DT
cord-328460-thx9zh11	184	44	fusion	fusion	NN
cord-328460-thx9zh11	184	45	of	of	IN
cord-328460-thx9zh11	184	46	the	the	DT
cord-328460-thx9zh11	184	47	2	2	CD
cord-328460-thx9zh11	184	48	pL	pl	NN
cord-328460-thx9zh11	184	49	droplet	droplet	NN
cord-328460-thx9zh11	184	50	with	with	IN
cord-328460-thx9zh11	184	51	a	a	DT
cord-328460-thx9zh11	184	52	15	15	CD
cord-328460-thx9zh11	184	53	pL	pl	NN
cord-328460-thx9zh11	184	54	droplet	droplet	NN
cord-328460-thx9zh11	184	55	containing	contain	VBG
cord-328460-thx9zh11	184	56	an	an	DT
cord-328460-thx9zh11	184	57	in	in	IN
cord-328460-thx9zh11	184	58	vitro	vitro	FW
cord-328460-thx9zh11	184	59	translation	translation	NN
cord-328460-thx9zh11	184	60	(	(	-LRB-
cord-328460-thx9zh11	184	61	IVT	IVT	NNP
cord-328460-thx9zh11	184	62	)	)	-RRB-
cord-328460-thx9zh11	184	63	system	system	NN
cord-328460-thx9zh11	184	64	supplemented	supplement	VBN
cord-328460-thx9zh11	184	65	with	with	IN
cord-328460-thx9zh11	184	66	fluorescein	fluorescein	NN
cord-328460-thx9zh11	184	67	-	-	HYPH
cord-328460-thx9zh11	184	68	di	di	NNP
cord-328460-thx9zh11	184	69	-	-	HYPH
cord-328460-thx9zh11	184	70	β	β	NN
cord-328460-thx9zh11	184	71	-	-	HYPH
cord-328460-thx9zh11	184	72	D	D	NNP
cord-328460-thx9zh11	184	73	-	-	HYPH
cord-328460-thx9zh11	184	74	galactopyranoside	galactopyranoside	NNP
cord-328460-thx9zh11	184	75	(	(	-LRB-
cord-328460-thx9zh11	184	76	FDG	FDG	NNP
cord-328460-thx9zh11	184	77	)	)	-RRB-
cord-328460-thx9zh11	184	78	that	that	WDT
cord-328460-thx9zh11	184	79	is	be	VBZ
cord-328460-thx9zh11	184	80	transformed	transform	VBN
cord-328460-thx9zh11	184	81	into	into	IN
cord-328460-thx9zh11	184	82	fluorescein	fluorescein	NN
cord-328460-thx9zh11	184	83	by	by	IN
cord-328460-thx9zh11	184	84	β	β	NN
cord-328460-thx9zh11	184	85	-	-	HYPH
cord-328460-thx9zh11	184	86	galactosidase	galactosidase	NN
cord-328460-thx9zh11	184	87	.	.	.
cord-328460-thx9zh11	185	1	Another	another	DT
cord-328460-thx9zh11	185	2	RCA	rca	NN
cord-328460-thx9zh11	185	3	-	-	HYPH
cord-328460-thx9zh11	185	4	based	base	VBN
cord-328460-thx9zh11	185	5	system	system	NN
cord-328460-thx9zh11	185	6	that	that	WDT
cord-328460-thx9zh11	185	7	benefits	benefit	VBZ
cord-328460-thx9zh11	185	8	from	from	IN
cord-328460-thx9zh11	185	9	the	the	DT
cord-328460-thx9zh11	185	10	typical	typical	JJ
cord-328460-thx9zh11	185	11	low	low	JJ
cord-328460-thx9zh11	185	12	sample	sample	NN
cord-328460-thx9zh11	185	13	consumption	consumption	NN
cord-328460-thx9zh11	185	14	and	and	CC
cord-328460-thx9zh11	185	15	short	short	JJ
cord-328460-thx9zh11	185	16	processing	processing	NN
cord-328460-thx9zh11	185	17	time	time	NN
cord-328460-thx9zh11	185	18	advantages	advantage	NNS
cord-328460-thx9zh11	185	19	of	of	IN
cord-328460-thx9zh11	185	20	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	185	21	is	be	VBZ
cord-328460-thx9zh11	185	22	the	the	DT
cord-328460-thx9zh11	185	23	rolling	rolling	JJ
cord-328460-thx9zh11	185	24	-	-	HYPH
cord-328460-thx9zh11	185	25	circle	circle	NN
cord-328460-thx9zh11	185	26	enhanced	enhanced	JJ
cord-328460-thx9zh11	185	27	enzyme	enzyme	NN
cord-328460-thx9zh11	185	28	activity	activity	NN
cord-328460-thx9zh11	185	29	detection	detection	NN
cord-328460-thx9zh11	185	30	(	(	-LRB-
cord-328460-thx9zh11	185	31	REEAD	REEAD	NNP
cord-328460-thx9zh11	185	32	)	)	-RRB-
cord-328460-thx9zh11	185	33	.	.	.
cord-328460-thx9zh11	186	1	REEAD	REEAD	NNP
cord-328460-thx9zh11	186	2	was	be	VBD
cord-328460-thx9zh11	186	3	combined	combine	VBN
cord-328460-thx9zh11	186	4	with	with	IN
cord-328460-thx9zh11	186	5	a	a	DT
cord-328460-thx9zh11	186	6	droplet	droplet	NN
cord-328460-thx9zh11	186	7	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	186	8	lab	lab	NN
cord-328460-thx9zh11	186	9	-	-	HYPH
cord-328460-thx9zh11	186	10	on	on	IN
cord-328460-thx9zh11	186	11	-	-	HYPH
cord-328460-thx9zh11	186	12	a	a	DT
cord-328460-thx9zh11	186	13	-	-	HYPH
cord-328460-thx9zh11	186	14	chip	chip	NN
cord-328460-thx9zh11	186	15	platform	platform	NN
cord-328460-thx9zh11	186	16	by	by	IN
cord-328460-thx9zh11	186	17	Juul	Juul	NNP
cord-328460-thx9zh11	186	18	et	et	NNP
cord-328460-thx9zh11	186	19	al	al	NNP
cord-328460-thx9zh11	186	20	.	.	.
cord-328460-thx9zh11	187	1	[	[	-LRB-
cord-328460-thx9zh11	187	2	91	91	CD
cord-328460-thx9zh11	187	3	]	]	-RRB-
cord-328460-thx9zh11	187	4	.	.	.
cord-328460-thx9zh11	188	1	The	the	DT
cord-328460-thx9zh11	188	2	principle	principle	NN
cord-328460-thx9zh11	188	3	of	of	IN
cord-328460-thx9zh11	188	4	REEAD	REEAD	NNP
cord-328460-thx9zh11	188	5	approach	approach	NN
cord-328460-thx9zh11	188	6	is	be	VBZ
cord-328460-thx9zh11	188	7	the	the	DT
cord-328460-thx9zh11	188	8	circularization	circularization	NN
cord-328460-thx9zh11	188	9	of	of	IN
cord-328460-thx9zh11	188	10	RCA	rca	NN
cord-328460-thx9zh11	188	11	substrate	substrate	NN
cord-328460-thx9zh11	188	12	by	by	IN
cord-328460-thx9zh11	188	13	the	the	DT
cord-328460-thx9zh11	188	14	Plasmodium	Plasmodium	NNP
cord-328460-thx9zh11	188	15	enzyme	enzyme	NN
cord-328460-thx9zh11	189	1	topoisomerase	topoisomerase	NN
cord-328460-thx9zh11	189	2	I	-PRON-	PRP
cord-328460-thx9zh11	189	3	(	(	-LRB-
cord-328460-thx9zh11	189	4	pTopI	ptopi	NN
cord-328460-thx9zh11	189	5	)	)	-RRB-
cord-328460-thx9zh11	189	6	together	together	RB
cord-328460-thx9zh11	189	7	with	with	IN
cord-328460-thx9zh11	189	8	the	the	DT
cord-328460-thx9zh11	189	9	conversion	conversion	NN
cord-328460-thx9zh11	189	10	of	of	IN
cord-328460-thx9zh11	189	11	single	single	JJ
cord-328460-thx9zh11	189	12	DNA	dna	NN
cord-328460-thx9zh11	189	13	cleavage	cleavage	NN
cord-328460-thx9zh11	189	14	-	-	HYPH
cord-328460-thx9zh11	189	15	ligation	ligation	NN
cord-328460-thx9zh11	189	16	events	event	NNS
cord-328460-thx9zh11	189	17	to	to	IN
cord-328460-thx9zh11	189	18	micrometer	micrometer	NN
cord-328460-thx9zh11	189	19	-	-	HYPH
cord-328460-thx9zh11	189	20	sized	sized	JJ
cord-328460-thx9zh11	189	21	products	product	NNS
cord-328460-thx9zh11	189	22	detectable	detectable	JJ
cord-328460-thx9zh11	189	23	at	at	IN
cord-328460-thx9zh11	189	24	the	the	DT
cord-328460-thx9zh11	189	25	single	single	JJ
cord-328460-thx9zh11	189	26	-	-	HYPH
cord-328460-thx9zh11	189	27	molecule	molecule	NN
cord-328460-thx9zh11	189	28	level	level	NN
cord-328460-thx9zh11	189	29	.	.	.
cord-328460-thx9zh11	190	1	The	the	DT
cord-328460-thx9zh11	190	2	REEAD	REEAD	NNP
cord-328460-thx9zh11	190	3	-	-	HYPH
cord-328460-thx9zh11	190	4	on	on	IN
cord-328460-thx9zh11	190	5	-	-	HYPH
cord-328460-thx9zh11	190	6	a	a	DT
cord-328460-thx9zh11	190	7	-	-	HYPH
cord-328460-thx9zh11	190	8	chip	chip	NN
cord-328460-thx9zh11	190	9	procedure	procedure	NN
cord-328460-thx9zh11	190	10	appears	appear	VBZ
cord-328460-thx9zh11	190	11	attractive	attractive	JJ
cord-328460-thx9zh11	190	12	since	since	IN
cord-328460-thx9zh11	190	13	it	-PRON-	PRP
cord-328460-thx9zh11	190	14	allows	allow	VBZ
cord-328460-thx9zh11	190	15	the	the	DT
cord-328460-thx9zh11	190	16	specific	specific	JJ
cord-328460-thx9zh11	190	17	,	,	,
cord-328460-thx9zh11	190	18	quantitative	quantitative	JJ
cord-328460-thx9zh11	190	19	and	and	CC
cord-328460-thx9zh11	190	20	highly	highly	RB
cord-328460-thx9zh11	190	21	sensitive	sensitive	JJ
cord-328460-thx9zh11	190	22	detection	detection	NN
cord-328460-thx9zh11	190	23	of	of	IN
cord-328460-thx9zh11	190	24	the	the	DT
cord-328460-thx9zh11	190	25	human	human	JJ
cord-328460-thx9zh11	190	26	-	-	HYPH
cord-328460-thx9zh11	190	27	malaria	malaria	NN
cord-328460-thx9zh11	190	28	-	-	HYPH
cord-328460-thx9zh11	190	29	causing	cause	VBG
cord-328460-thx9zh11	190	30	Plasmodium	plasmodium	NN
cord-328460-thx9zh11	190	31	parasites	parasite	NNS
cord-328460-thx9zh11	190	32	in	in	IN
cord-328460-thx9zh11	190	33	small	small	JJ
cord-328460-thx9zh11	190	34	volumes	volume	NNS
cord-328460-thx9zh11	190	35	(	(	-LRB-
cord-328460-thx9zh11	190	36	pL	pL	NNP
cord-328460-thx9zh11	190	37	droplets	droplet	NNS
cord-328460-thx9zh11	190	38	)	)	-RRB-
cord-328460-thx9zh11	190	39	of	of	IN
cord-328460-thx9zh11	190	40	unprocessed	unprocessed	JJ
cord-328460-thx9zh11	190	41	and	and	CC
cord-328460-thx9zh11	190	42	crude	crude	JJ
cord-328460-thx9zh11	190	43	clinical	clinical	JJ
cord-328460-thx9zh11	190	44	samples	sample	NNS
cord-328460-thx9zh11	190	45	with	with	IN
cord-328460-thx9zh11	190	46	a	a	DT
cord-328460-thx9zh11	190	47	detection	detection	NN
cord-328460-thx9zh11	190	48	limit	limit	NN
cord-328460-thx9zh11	190	49	of	of	IN
cord-328460-thx9zh11	190	50	less	less	JJR
cord-328460-thx9zh11	190	51	than	than	IN
cord-328460-thx9zh11	190	52	one	one	CD
cord-328460-thx9zh11	190	53	parasite/	parasite/	NN
cord-328460-thx9zh11	191	1	μL.	μL.	NNP
cord-328460-thx9zh11	191	2	MDA	MDA	NNP
cord-328460-thx9zh11	191	3	is	be	VBZ
cord-328460-thx9zh11	191	4	an	an	DT
cord-328460-thx9zh11	191	5	isothermal	isothermal	JJ
cord-328460-thx9zh11	191	6	method	method	NN
cord-328460-thx9zh11	191	7	for	for	IN
cord-328460-thx9zh11	191	8	whole	whole	JJ
cord-328460-thx9zh11	191	9	genome	genome	NN
cord-328460-thx9zh11	191	10	amplification	amplification	NN
cord-328460-thx9zh11	191	11	(	(	-LRB-
cord-328460-thx9zh11	191	12	WGA	WGA	NNP
cord-328460-thx9zh11	191	13	)	)	-RRB-
cord-328460-thx9zh11	191	14	capable	capable	JJ
cord-328460-thx9zh11	191	15	of	of	IN
cord-328460-thx9zh11	191	16	producing	produce	VBG
cord-328460-thx9zh11	191	17	a	a	DT
cord-328460-thx9zh11	191	18	large	large	JJ
cord-328460-thx9zh11	191	19	number	number	NN
cord-328460-thx9zh11	191	20	of	of	IN
cord-328460-thx9zh11	191	21	amplification	amplification	NN
cord-328460-thx9zh11	191	22	products	product	NNS
cord-328460-thx9zh11	191	23	from	from	IN
cord-328460-thx9zh11	191	24	a	a	DT
cord-328460-thx9zh11	191	25	few	few	JJ
cord-328460-thx9zh11	191	26	DNA	dna	NN
cord-328460-thx9zh11	191	27	molecules	molecule	NNS
cord-328460-thx9zh11	191	28	[	[	-LRB-
cord-328460-thx9zh11	191	29	92	92	CD
cord-328460-thx9zh11	191	30	]	]	-RRB-
cord-328460-thx9zh11	191	31	.	.	.
cord-328460-thx9zh11	192	1	MDA	MDA	NNP
cord-328460-thx9zh11	192	2	exploits	exploit	VBZ
cord-328460-thx9zh11	192	3	both	both	DT
cord-328460-thx9zh11	192	4	random	random	JJ
cord-328460-thx9zh11	192	5	exonuclease	exonuclease	NN
cord-328460-thx9zh11	192	6	-	-	HYPH
cord-328460-thx9zh11	192	7	resistant	resistant	JJ
cord-328460-thx9zh11	192	8	primers	primer	NNS
cord-328460-thx9zh11	192	9	as	as	RB
cord-328460-thx9zh11	192	10	well	well	RB
cord-328460-thx9zh11	192	11	as	as	IN
cord-328460-thx9zh11	192	12	the	the	DT
cord-328460-thx9zh11	192	13	strand	strand	NN
cord-328460-thx9zh11	192	14	displacement	displacement	NN
cord-328460-thx9zh11	192	15	activity	activity	NN
cord-328460-thx9zh11	192	16	of	of	IN
cord-328460-thx9zh11	192	17	φ29	φ29	NN
cord-328460-thx9zh11	192	18	DNA	dna	NN
cord-328460-thx9zh11	192	19	polymerase	polymerase	NN
cord-328460-thx9zh11	192	20	to	to	TO
cord-328460-thx9zh11	192	21	produce	produce	VB
cord-328460-thx9zh11	192	22	DNA	dna	NN
cord-328460-thx9zh11	192	23	strands	strand	NNS
cord-328460-thx9zh11	192	24	70	70	CD
cord-328460-thx9zh11	192	25	kb	kb	NNS
cord-328460-thx9zh11	192	26	in	in	IN
cord-328460-thx9zh11	192	27	length	length	NN
cord-328460-thx9zh11	192	28	[	[	-LRB-
cord-328460-thx9zh11	192	29	93	93	CD
cord-328460-thx9zh11	192	30	]	]	-RRB-
cord-328460-thx9zh11	192	31	with	with	IN
cord-328460-thx9zh11	192	32	no	no	DT
cord-328460-thx9zh11	192	33	need	need	NN
cord-328460-thx9zh11	192	34	for	for	IN
cord-328460-thx9zh11	192	35	thermal	thermal	JJ
cord-328460-thx9zh11	192	36	cycling	cycling	NN
cord-328460-thx9zh11	192	37	.	.	.
cord-328460-thx9zh11	193	1	Random	random	JJ
cord-328460-thx9zh11	193	2	primers	primer	NNS
cord-328460-thx9zh11	193	3	and	and	CC
cord-328460-thx9zh11	193	4	φ29	φ29	NN
cord-328460-thx9zh11	193	5	DNA	dna	NN
cord-328460-thx9zh11	193	6	polymerase	polymerase	NN
cord-328460-thx9zh11	193	7	are	be	VBP
cord-328460-thx9zh11	193	8	already	already	RB
cord-328460-thx9zh11	193	9	used	use	VBN
cord-328460-thx9zh11	193	10	for	for	IN
cord-328460-thx9zh11	193	11	circular	circular	JJ
cord-328460-thx9zh11	193	12	DNA	dna	NN
cord-328460-thx9zh11	193	13	amplification	amplification	NN
cord-328460-thx9zh11	193	14	in	in	IN
cord-328460-thx9zh11	193	15	RCA	RCA	NNP
cord-328460-thx9zh11	193	16	[	[	-LRB-
cord-328460-thx9zh11	193	17	85	85	CD
cord-328460-thx9zh11	193	18	,	,	,
cord-328460-thx9zh11	193	19	86	86	CD
cord-328460-thx9zh11	193	20	]	]	-RRB-
cord-328460-thx9zh11	193	21	.	.	.
cord-328460-thx9zh11	194	1	In	in	IN
cord-328460-thx9zh11	194	2	MDA	MDA	NNP
cord-328460-thx9zh11	194	3	the	the	DT
cord-328460-thx9zh11	194	4	above	above	RB
cord-328460-thx9zh11	194	5	-	-	HYPH
cord-328460-thx9zh11	194	6	mentioned	mention	VBN
cord-328460-thx9zh11	194	7	components	component	NNS
cord-328460-thx9zh11	194	8	are	be	VBP
cord-328460-thx9zh11	194	9	employed	employ	VBN
cord-328460-thx9zh11	194	10	to	to	TO
cord-328460-thx9zh11	194	11	amplify	amplify	VB
cord-328460-thx9zh11	194	12	linear	linear	JJ
cord-328460-thx9zh11	194	13	genomic	genomic	JJ
cord-328460-thx9zh11	194	14	DNA	dna	NN
cord-328460-thx9zh11	194	15	.	.	.
cord-328460-thx9zh11	195	1	The	the	DT
cord-328460-thx9zh11	195	2	use	use	NN
cord-328460-thx9zh11	195	3	of	of	IN
cord-328460-thx9zh11	195	4	a	a	DT
cord-328460-thx9zh11	195	5	high	high	JJ
cord-328460-thx9zh11	195	6	-	-	HYPH
cord-328460-thx9zh11	195	7	fidelity	fidelity	NN
cord-328460-thx9zh11	195	8	proofreading	proofreading	NN
cord-328460-thx9zh11	195	9	DNA	dna	NN
cord-328460-thx9zh11	195	10	polymerase	polymerase	NN
cord-328460-thx9zh11	195	11	ensures	ensure	VBZ
cord-328460-thx9zh11	195	12	a	a	DT
cord-328460-thx9zh11	195	13	significantly	significantly	RB
cord-328460-thx9zh11	195	14	lower	low	JJR
cord-328460-thx9zh11	195	15	error	error	NN
cord-328460-thx9zh11	195	16	rate	rate	NN
cord-328460-thx9zh11	195	17	during	during	IN
cord-328460-thx9zh11	195	18	the	the	DT
cord-328460-thx9zh11	195	19	amplification	amplification	NN
cord-328460-thx9zh11	195	20	reaction	reaction	NN
cord-328460-thx9zh11	195	21	(	(	-LRB-
cord-328460-thx9zh11	195	22	3	3	CD
cord-328460-thx9zh11	195	23	×	×	NN
cord-328460-thx9zh11	195	24	10	10	CD
cord-328460-thx9zh11	195	25	−6	−6	NNP
cord-328460-thx9zh11	195	26	mutations	mutation	NNS
cord-328460-thx9zh11	195	27	/	/	SYM
cord-328460-thx9zh11	195	28	nucleotide	nucleotide	NN
cord-328460-thx9zh11	195	29	)	)	-RRB-
cord-328460-thx9zh11	195	30	[	[	-LRB-
cord-328460-thx9zh11	195	31	94	94	CD
cord-328460-thx9zh11	195	32	]	]	-RRB-
cord-328460-thx9zh11	195	33	in	in	IN
cord-328460-thx9zh11	195	34	comparison	comparison	NN
cord-328460-thx9zh11	195	35	with	with	IN
cord-328460-thx9zh11	195	36	Taq	Taq	NNP
cord-328460-thx9zh11	195	37	DNA	dna	NN
cord-328460-thx9zh11	195	38	polymerase	polymerase	NN
cord-328460-thx9zh11	195	39	used	use	VBN
cord-328460-thx9zh11	195	40	for	for	IN
cord-328460-thx9zh11	195	41	PCR	PCR	NNP
cord-328460-thx9zh11	195	42	amplification	amplification	NN
cord-328460-thx9zh11	195	43	(	(	-LRB-
cord-328460-thx9zh11	195	44	~2	~2	NNP
cord-328460-thx9zh11	195	45	×	×	NN
cord-328460-thx9zh11	195	46	10	10	CD
cord-328460-thx9zh11	196	1	−4	−4	CD
cord-328460-thx9zh11	197	1	mutations	mutation	NNS
cord-328460-thx9zh11	197	2	/	/	SYM
cord-328460-thx9zh11	197	3	nucleotide	nucleotide	NN
cord-328460-thx9zh11	197	4	)	)	-RRB-
cord-328460-thx9zh11	198	1	[	[	-LRB-
cord-328460-thx9zh11	198	2	95	95	CD
cord-328460-thx9zh11	198	3	,	,	,
cord-328460-thx9zh11	198	4	96	96	CD
cord-328460-thx9zh11	198	5	]	]	-RRB-
cord-328460-thx9zh11	198	6	.	.	.
cord-328460-thx9zh11	199	1	The	the	DT
cord-328460-thx9zh11	199	2	high	high	JJ
cord-328460-thx9zh11	199	3	-	-	HYPH
cord-328460-thx9zh11	199	4	fidelity	fidelity	NN
cord-328460-thx9zh11	199	5	amplification	amplification	NN
cord-328460-thx9zh11	199	6	by	by	IN
cord-328460-thx9zh11	199	7	MDA	MDA	NNP
cord-328460-thx9zh11	199	8	preserves	preserve	VBZ
cord-328460-thx9zh11	199	9	DNA	dna	NN
cord-328460-thx9zh11	199	10	sequence	sequence	NN
cord-328460-thx9zh11	199	11	information	information	NN
cord-328460-thx9zh11	199	12	,	,	,
cord-328460-thx9zh11	199	13	assuring	assure	VBG
cord-328460-thx9zh11	199	14	accurate	accurate	JJ
cord-328460-thx9zh11	199	15	genotyping	genotyping	NN
cord-328460-thx9zh11	199	16	in	in	IN
cord-328460-thx9zh11	199	17	downstream	downstream	JJ
cord-328460-thx9zh11	199	18	applications	application	NNS
cord-328460-thx9zh11	199	19	regardless	regardless	RB
cord-328460-thx9zh11	199	20	of	of	IN
cord-328460-thx9zh11	199	21	the	the	DT
cord-328460-thx9zh11	199	22	type	type	NN
cord-328460-thx9zh11	199	23	of	of	IN
cord-328460-thx9zh11	199	24	clinical	clinical	JJ
cord-328460-thx9zh11	199	25	sample	sample	NN
cord-328460-thx9zh11	199	26	used	use	VBN
cord-328460-thx9zh11	199	27	including	include	VBG
cord-328460-thx9zh11	199	28	buccal	buccal	JJ
cord-328460-thx9zh11	199	29	swabs	swab	NNS
cord-328460-thx9zh11	199	30	,	,	,
cord-328460-thx9zh11	199	31	whole	whole	JJ
cord-328460-thx9zh11	199	32	blood	blood	NN
cord-328460-thx9zh11	199	33	,	,	,
cord-328460-thx9zh11	199	34	finger	finger	NN
cord-328460-thx9zh11	199	35	stick	stick	VB
cord-328460-thx9zh11	199	36	blood	blood	NN
cord-328460-thx9zh11	199	37	and	and	CC
cord-328460-thx9zh11	199	38	Guthrie	Guthrie	NNP
cord-328460-thx9zh11	199	39	cards	card	NNS
cord-328460-thx9zh11	199	40	[	[	-LRB-
cord-328460-thx9zh11	199	41	97	97	CD
cord-328460-thx9zh11	199	42	]	]	-RRB-
cord-328460-thx9zh11	199	43	.	.	.
cord-328460-thx9zh11	200	1	MDA	MDA	NNP
cord-328460-thx9zh11	200	2	does	do	VBZ
cord-328460-thx9zh11	200	3	not	not	RB
cord-328460-thx9zh11	200	4	require	require	VB
cord-328460-thx9zh11	200	5	the	the	DT
cord-328460-thx9zh11	200	6	temperature	temperature	NN
cord-328460-thx9zh11	200	7	-	-	HYPH
cord-328460-thx9zh11	200	8	modulated	modulate	VBN
cord-328460-thx9zh11	200	9	denaturation	denaturation	NN
cord-328460-thx9zh11	200	10	of	of	IN
cord-328460-thx9zh11	200	11	the	the	DT
cord-328460-thx9zh11	200	12	genomic	genomic	JJ
cord-328460-thx9zh11	200	13	DNA	dna	NN
cord-328460-thx9zh11	200	14	to	to	TO
cord-328460-thx9zh11	200	15	facilitate	facilitate	VB
cord-328460-thx9zh11	200	16	the	the	DT
cord-328460-thx9zh11	200	17	annealing	annealing	NN
cord-328460-thx9zh11	200	18	of	of	IN
cord-328460-thx9zh11	200	19	random	random	JJ
cord-328460-thx9zh11	200	20	primers	primer	NNS
cord-328460-thx9zh11	200	21	.	.	.
cord-328460-thx9zh11	201	1	In	in	IN
cord-328460-thx9zh11	201	2	fact	fact	NN
cord-328460-thx9zh11	201	3	,	,	,
cord-328460-thx9zh11	201	4	the	the	DT
cord-328460-thx9zh11	201	5	random	random	JJ
cord-328460-thx9zh11	201	6	priming	priming	NN
cord-328460-thx9zh11	201	7	on	on	IN
cord-328460-thx9zh11	201	8	the	the	DT
cord-328460-thx9zh11	201	9	double	double	RB
cord-328460-thx9zh11	201	10	-	-	HYPH
cord-328460-thx9zh11	201	11	stranded	strand	VBN
cord-328460-thx9zh11	201	12	DNA	dna	NN
cord-328460-thx9zh11	201	13	target	target	NN
cord-328460-thx9zh11	201	14	is	be	VBZ
cord-328460-thx9zh11	201	15	efficient	efficient	JJ
cord-328460-thx9zh11	201	16	enough	enough	RB
cord-328460-thx9zh11	201	17	to	to	TO
cord-328460-thx9zh11	201	18	obtain	obtain	VB
cord-328460-thx9zh11	201	19	an	an	DT
cord-328460-thx9zh11	201	20	initial	initial	JJ
cord-328460-thx9zh11	201	21	priming	priming	NN
cord-328460-thx9zh11	201	22	step	step	NN
cord-328460-thx9zh11	201	23	that	that	WDT
cord-328460-thx9zh11	201	24	is	be	VBZ
cord-328460-thx9zh11	201	25	followed	follow	VBN
cord-328460-thx9zh11	201	26	by	by	IN
cord-328460-thx9zh11	201	27	the	the	DT
cord-328460-thx9zh11	201	28	strand	strand	NN
cord-328460-thx9zh11	201	29	-	-	HYPH
cord-328460-thx9zh11	201	30	displacement	displacement	NN
cord-328460-thx9zh11	201	31	process	process	NN
cord-328460-thx9zh11	201	32	that	that	WDT
cord-328460-thx9zh11	201	33	produces	produce	VBZ
cord-328460-thx9zh11	201	34	the	the	DT
cord-328460-thx9zh11	201	35	amplification	amplification	NN
cord-328460-thx9zh11	201	36	.	.	.
cord-328460-thx9zh11	202	1	It	-PRON-	PRP
cord-328460-thx9zh11	202	2	has	have	VBZ
cord-328460-thx9zh11	202	3	been	be	VBN
cord-328460-thx9zh11	202	4	also	also	RB
cord-328460-thx9zh11	202	5	demonstrated	demonstrate	VBN
cord-328460-thx9zh11	202	6	that	that	IN
cord-328460-thx9zh11	202	7	the	the	DT
cord-328460-thx9zh11	202	8	omission	omission	NN
cord-328460-thx9zh11	202	9	of	of	IN
cord-328460-thx9zh11	202	10	the	the	DT
cord-328460-thx9zh11	202	11	denaturation	denaturation	NN
cord-328460-thx9zh11	202	12	step	step	NN
cord-328460-thx9zh11	202	13	results	result	NNS
cord-328460-thx9zh11	202	14	in	in	IN
cord-328460-thx9zh11	202	15	a	a	DT
cord-328460-thx9zh11	202	16	reduced	reduce	VBN
cord-328460-thx9zh11	202	17	template	template	NN
cord-328460-thx9zh11	202	18	degradation	degradation	NN
cord-328460-thx9zh11	202	19	and	and	CC
cord-328460-thx9zh11	202	20	in	in	IN
cord-328460-thx9zh11	202	21	an	an	DT
cord-328460-thx9zh11	202	22	improved	improved	JJ
cord-328460-thx9zh11	202	23	specificity	specificity	NN
cord-328460-thx9zh11	202	24	of	of	IN
cord-328460-thx9zh11	202	25	the	the	DT
cord-328460-thx9zh11	202	26	amplification	amplification	NN
cord-328460-thx9zh11	202	27	[	[	-LRB-
cord-328460-thx9zh11	202	28	92	92	CD
cord-328460-thx9zh11	202	29	]	]	-RRB-
cord-328460-thx9zh11	202	30	.	.	.
cord-328460-thx9zh11	203	1	MDA	MDA	NNP
cord-328460-thx9zh11	203	2	has	have	VBZ
cord-328460-thx9zh11	203	3	been	be	VBN
cord-328460-thx9zh11	203	4	directly	directly	RB
cord-328460-thx9zh11	203	5	used	use	VBN
cord-328460-thx9zh11	203	6	on	on	IN
cord-328460-thx9zh11	203	7	lysed	lyse	VBN
cord-328460-thx9zh11	203	8	cells	cell	NNS
cord-328460-thx9zh11	203	9	thus	thus	RB
cord-328460-thx9zh11	203	10	avoids	avoid	VBZ
cord-328460-thx9zh11	203	11	the	the	DT
cord-328460-thx9zh11	203	12	DNA	dna	NN
cord-328460-thx9zh11	203	13	template	template	NN
cord-328460-thx9zh11	203	14	loss	loss	NN
cord-328460-thx9zh11	203	15	and	and	CC
cord-328460-thx9zh11	203	16	damage	damage	NN
cord-328460-thx9zh11	203	17	and	and	CC
cord-328460-thx9zh11	203	18	the	the	DT
cord-328460-thx9zh11	203	19	risk	risk	NN
cord-328460-thx9zh11	203	20	of	of	IN
cord-328460-thx9zh11	203	21	contamination	contamination	NN
cord-328460-thx9zh11	203	22	that	that	WDT
cord-328460-thx9zh11	203	23	can	can	MD
cord-328460-thx9zh11	203	24	occur	occur	VB
cord-328460-thx9zh11	203	25	in	in	IN
cord-328460-thx9zh11	203	26	DNA	dna	NN
cord-328460-thx9zh11	203	27	purification	purification	NN
cord-328460-thx9zh11	203	28	processes	process	NNS
cord-328460-thx9zh11	203	29	[	[	-LRB-
cord-328460-thx9zh11	203	30	97	97	CD
cord-328460-thx9zh11	203	31	]	]	-RRB-
cord-328460-thx9zh11	203	32	.	.	.
cord-328460-thx9zh11	204	1	The	the	DT
cord-328460-thx9zh11	204	2	possibility	possibility	NN
cord-328460-thx9zh11	204	3	to	to	TO
cord-328460-thx9zh11	204	4	directly	directly	RB
cord-328460-thx9zh11	204	5	apply	apply	VB
cord-328460-thx9zh11	204	6	MDA	MDA	NNP
cord-328460-thx9zh11	204	7	to	to	TO
cord-328460-thx9zh11	204	8	genomic	genomic	JJ
cord-328460-thx9zh11	204	9	material	material	NN
cord-328460-thx9zh11	204	10	obtained	obtain	VBN
cord-328460-thx9zh11	204	11	from	from	IN
cord-328460-thx9zh11	204	12	single	single	JJ
cord-328460-thx9zh11	204	13	cells	cell	NNS
cord-328460-thx9zh11	204	14	helps	help	VBZ
cord-328460-thx9zh11	204	15	in	in	IN
cord-328460-thx9zh11	204	16	eliminating	eliminate	VBG
cord-328460-thx9zh11	204	17	the	the	DT
cord-328460-thx9zh11	204	18	need	need	NN
cord-328460-thx9zh11	204	19	to	to	TO
cord-328460-thx9zh11	204	20	develop	develop	VB
cord-328460-thx9zh11	204	21	culture	culture	NN
cord-328460-thx9zh11	204	22	methods	method	NNS
cord-328460-thx9zh11	204	23	by	by	IN
cord-328460-thx9zh11	204	24	also	also	RB
cord-328460-thx9zh11	204	25	allowing	allow	VBG
cord-328460-thx9zh11	204	26	the	the	DT
cord-328460-thx9zh11	204	27	direct	direct	JJ
cord-328460-thx9zh11	204	28	analysis	analysis	NN
cord-328460-thx9zh11	204	29	of	of	IN
cord-328460-thx9zh11	204	30	genomic	genomic	JJ
cord-328460-thx9zh11	204	31	DNA	dna	NN
cord-328460-thx9zh11	204	32	from	from	IN
cord-328460-thx9zh11	204	33	nonculturable	nonculturable	JJ
cord-328460-thx9zh11	204	34	bacteria	bacteria	NNS
cord-328460-thx9zh11	204	35	.	.	.
cord-328460-thx9zh11	205	1	MDA	MDA	NNP
cord-328460-thx9zh11	205	2	has	have	VBZ
cord-328460-thx9zh11	205	3	been	be	VBN
cord-328460-thx9zh11	205	4	shown	show	VBN
cord-328460-thx9zh11	205	5	to	to	TO
cord-328460-thx9zh11	205	6	produce	produce	VB
cord-328460-thx9zh11	205	7	several	several	JJ
cord-328460-thx9zh11	205	8	-	-	HYPH
cord-328460-thx9zh11	205	9	billion	billion	CD
cord-328460-thx9zh11	205	10	-	-	HYPH
cord-328460-thx9zh11	205	11	fold	fold	JJ
cord-328460-thx9zh11	205	12	amplification	amplification	NN
cord-328460-thx9zh11	205	13	of	of	IN
cord-328460-thx9zh11	205	14	genomic	genomic	JJ
cord-328460-thx9zh11	205	15	DNA	dna	NN
cord-328460-thx9zh11	205	16	from	from	IN
cord-328460-thx9zh11	205	17	single	single	JJ
cord-328460-thx9zh11	205	18	,	,	,
cord-328460-thx9zh11	205	19	flow	flow	NN
cord-328460-thx9zh11	205	20	-	-	HYPH
cord-328460-thx9zh11	205	21	sorted	sort	VBN
cord-328460-thx9zh11	205	22	bacterial	bacterial	JJ
cord-328460-thx9zh11	205	23	cells	cell	NNS
cord-328460-thx9zh11	205	24	[	[	-LRB-
cord-328460-thx9zh11	205	25	98	98	CD
cord-328460-thx9zh11	205	26	]	]	-RRB-
cord-328460-thx9zh11	205	27	.	.	.
cord-328460-thx9zh11	206	1	Unfortunately	unfortunately	RB
cord-328460-thx9zh11	206	2	,	,	,
cord-328460-thx9zh11	206	3	only	only	RB
cord-328460-thx9zh11	206	4	about	about	RB
cord-328460-thx9zh11	206	5	30	30	CD
cord-328460-thx9zh11	206	6	%	%	NN
cord-328460-thx9zh11	206	7	of	of	IN
cord-328460-thx9zh11	206	8	the	the	DT
cord-328460-thx9zh11	206	9	DNA	dna	NN
cord-328460-thx9zh11	206	10	amplification	amplification	NN
cord-328460-thx9zh11	206	11	was	be	VBD
cord-328460-thx9zh11	206	12	specific	specific	JJ
cord-328460-thx9zh11	206	13	because	because	IN
cord-328460-thx9zh11	206	14	MDA	MDA	NNP
cord-328460-thx9zh11	206	15	tends	tend	VBZ
cord-328460-thx9zh11	206	16	to	to	TO
cord-328460-thx9zh11	206	17	amplify	amplify	VB
cord-328460-thx9zh11	206	18	all	all	DT
cord-328460-thx9zh11	206	19	of	of	IN
cord-328460-thx9zh11	206	20	the	the	DT
cord-328460-thx9zh11	206	21	DNA	dna	NN
cord-328460-thx9zh11	206	22	present	present	NN
cord-328460-thx9zh11	206	23	in	in	IN
cord-328460-thx9zh11	206	24	a	a	DT
cord-328460-thx9zh11	206	25	sample	sample	NN
cord-328460-thx9zh11	206	26	,	,	,
cord-328460-thx9zh11	206	27	including	include	VBG
cord-328460-thx9zh11	206	28	contaminating	contaminate	VBG
cord-328460-thx9zh11	206	29	DNAs	dna	NNS
cord-328460-thx9zh11	206	30	.	.	.
cord-328460-thx9zh11	207	1	By	by	IN
cord-328460-thx9zh11	207	2	using	use	VBG
cord-328460-thx9zh11	207	3	a	a	DT
cord-328460-thx9zh11	207	4	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	207	5	device	device	NN
cord-328460-thx9zh11	207	6	comprising	comprise	VBG
cord-328460-thx9zh11	207	7	60	60	CD
cord-328460-thx9zh11	207	8	nl	nl	NNP
cord-328460-thx9zh11	207	9	reactors	reactor	NNS
cord-328460-thx9zh11	207	10	and	and	CC
cord-328460-thx9zh11	207	11	an	an	DT
cord-328460-thx9zh11	207	12	integrated	integrated	JJ
cord-328460-thx9zh11	207	13	cell	cell	NN
cord-328460-thx9zh11	207	14	sorter	sorter	NN
cord-328460-thx9zh11	207	15	to	to	TO
cord-328460-thx9zh11	207	16	isolate	isolate	VB
cord-328460-thx9zh11	207	17	selected	select	VBN
cord-328460-thx9zh11	207	18	individual	individual	JJ
cord-328460-thx9zh11	207	19	cells	cell	NNS
cord-328460-thx9zh11	207	20	,	,	,
cord-328460-thx9zh11	207	21	it	-PRON-	PRP
cord-328460-thx9zh11	207	22	has	have	VBZ
cord-328460-thx9zh11	207	23	been	be	VBN
cord-328460-thx9zh11	207	24	shown	show	VBN
cord-328460-thx9zh11	207	25	that	that	IN
cord-328460-thx9zh11	207	26	the	the	DT
cord-328460-thx9zh11	207	27	amplification	amplification	NN
cord-328460-thx9zh11	207	28	bias	bias	NN
cord-328460-thx9zh11	207	29	can	can	MD
cord-328460-thx9zh11	207	30	be	be	VB
cord-328460-thx9zh11	207	31	reduced	reduce	VBN
cord-328460-thx9zh11	207	32	and	and	CC
cord-328460-thx9zh11	207	33	the	the	DT
cord-328460-thx9zh11	207	34	specificity	specificity	NN
cord-328460-thx9zh11	207	35	of	of	IN
cord-328460-thx9zh11	207	36	the	the	DT
cord-328460-thx9zh11	207	37	amplification	amplification	NN
cord-328460-thx9zh11	207	38	process	process	NN
cord-328460-thx9zh11	207	39	can	can	MD
cord-328460-thx9zh11	207	40	be	be	VB
cord-328460-thx9zh11	207	41	increased	increase	VBN
cord-328460-thx9zh11	207	42	as	as	IN
cord-328460-thx9zh11	207	43	the	the	DT
cord-328460-thx9zh11	207	44	reaction	reaction	NN
cord-328460-thx9zh11	207	45	volume	volume	NN
cord-328460-thx9zh11	207	46	shrinks	shrink	VBZ
cord-328460-thx9zh11	207	47	from	from	IN
cord-328460-thx9zh11	207	48	microliters	microliter	NNS
cord-328460-thx9zh11	207	49	to	to	IN
cord-328460-thx9zh11	207	50	nanoliters	nanoliter	NNS
cord-328460-thx9zh11	207	51	[	[	-LRB-
cord-328460-thx9zh11	207	52	99	99	CD
cord-328460-thx9zh11	207	53	]	]	-RRB-
cord-328460-thx9zh11	207	54	.	.	.
cord-328460-thx9zh11	208	1	Reduced	reduce	VBN
cord-328460-thx9zh11	208	2	MDA	MDA	NNP
cord-328460-thx9zh11	208	3	reaction	reaction	NN
cord-328460-thx9zh11	208	4	volumes	volume	NNS
cord-328460-thx9zh11	208	5	lower	lower	VBP
cord-328460-thx9zh11	208	6	nonspecific	nonspecific	JJ
cord-328460-thx9zh11	208	7	amplification	amplification	NN
cord-328460-thx9zh11	208	8	that	that	WDT
cord-328460-thx9zh11	208	9	can	can	MD
cord-328460-thx9zh11	208	10	result	result	VB
cord-328460-thx9zh11	208	11	from	from	IN
cord-328460-thx9zh11	208	12	contaminant	contaminant	NN
cord-328460-thx9zh11	208	13	DNA	dna	NN
cord-328460-thx9zh11	208	14	templates	template	NNS
cord-328460-thx9zh11	208	15	or	or	CC
cord-328460-thx9zh11	208	16	unfavorable	unfavorable	JJ
cord-328460-thx9zh11	208	17	interactions	interaction	NNS
cord-328460-thx9zh11	208	18	between	between	IN
cord-328460-thx9zh11	208	19	primers	primer	NNS
cord-328460-thx9zh11	208	20	[	[	-LRB-
cord-328460-thx9zh11	208	21	100	100	CD
cord-328460-thx9zh11	208	22	]	]	-RRB-
cord-328460-thx9zh11	208	23	.	.	.
cord-328460-thx9zh11	209	1	It	-PRON-	PRP
cord-328460-thx9zh11	209	2	is	be	VBZ
cord-328460-thx9zh11	209	3	important	important	JJ
cord-328460-thx9zh11	209	4	to	to	TO
cord-328460-thx9zh11	209	5	recognize	recognize	VB
cord-328460-thx9zh11	209	6	that	that	IN
cord-328460-thx9zh11	209	7	MDA	MDA	NNP
cord-328460-thx9zh11	209	8	tends	tend	VBZ
cord-328460-thx9zh11	209	9	to	to	TO
cord-328460-thx9zh11	209	10	be	be	VB
cord-328460-thx9zh11	209	11	a	a	DT
cord-328460-thx9zh11	209	12	self	self	NN
cord-328460-thx9zh11	209	13	-	-	HYPH
cord-328460-thx9zh11	209	14	limiting	limit	VBG
cord-328460-thx9zh11	209	15	reaction	reaction	NN
cord-328460-thx9zh11	209	16	.	.	.
cord-328460-thx9zh11	210	1	The	the	DT
cord-328460-thx9zh11	210	2	amplification	amplification	NN
cord-328460-thx9zh11	210	3	yield	yield	NN
cord-328460-thx9zh11	210	4	is	be	VBZ
cord-328460-thx9zh11	210	5	independent	independent	JJ
cord-328460-thx9zh11	210	6	of	of	IN
cord-328460-thx9zh11	210	7	the	the	DT
cord-328460-thx9zh11	210	8	input	input	NN
cord-328460-thx9zh11	210	9	amount	amount	NN
cord-328460-thx9zh11	210	10	of	of	IN
cord-328460-thx9zh11	210	11	DNA	dna	NN
cord-328460-thx9zh11	210	12	template	template	NN
cord-328460-thx9zh11	210	13	and	and	CC
cord-328460-thx9zh11	210	14	reaches	reach	VBZ
cord-328460-thx9zh11	210	15	a	a	DT
cord-328460-thx9zh11	210	16	plateau	plateau	NN
cord-328460-thx9zh11	210	17	at	at	IN
cord-328460-thx9zh11	210	18	a	a	DT
cord-328460-thx9zh11	210	19	0.7	0.7	CD
cord-328460-thx9zh11	210	20	-	-	SYM
cord-328460-thx9zh11	210	21	1.0	1.0	CD
cord-328460-thx9zh11	210	22	µg/µL	µg/µL	NNP
cord-328460-thx9zh11	210	23	concentration	concentration	NN
cord-328460-thx9zh11	210	24	of	of	IN
cord-328460-thx9zh11	210	25	the	the	DT
cord-328460-thx9zh11	210	26	amplified	amplify	VBN
cord-328460-thx9zh11	210	27	DNA	DNA	NNP
cord-328460-thx9zh11	210	28	[	[	-LRB-
cord-328460-thx9zh11	210	29	97	97	CD
cord-328460-thx9zh11	210	30	]	]	-RRB-
cord-328460-thx9zh11	210	31	.	.	.
cord-328460-thx9zh11	211	1	RPA	RPA	NNP
cord-328460-thx9zh11	211	2	is	be	VBZ
cord-328460-thx9zh11	211	3	a	a	DT
cord-328460-thx9zh11	211	4	low	low	JJ
cord-328460-thx9zh11	211	5	temperature	temperature	NN
cord-328460-thx9zh11	211	6	(	(	-LRB-
cord-328460-thx9zh11	211	7	about	about	RB
cord-328460-thx9zh11	211	8	37	37	CD
cord-328460-thx9zh11	211	9	°	°	NN
cord-328460-thx9zh11	211	10	C	c	NN
cord-328460-thx9zh11	211	11	)	)	-RRB-
cord-328460-thx9zh11	211	12	isothermal	isothermal	JJ
cord-328460-thx9zh11	211	13	amplification	amplification	NN
cord-328460-thx9zh11	211	14	method	method	NN
cord-328460-thx9zh11	211	15	that	that	WDT
cord-328460-thx9zh11	211	16	couples	couple	VBZ
cord-328460-thx9zh11	211	17	the	the	DT
cord-328460-thx9zh11	211	18	isothermal	isothermal	JJ
cord-328460-thx9zh11	211	19	recombinase	recombinase	NN
cord-328460-thx9zh11	211	20	-	-	HYPH
cord-328460-thx9zh11	211	21	driven	drive	VBN
cord-328460-thx9zh11	211	22	primer	primer	NN
cord-328460-thx9zh11	211	23	targeting	targeting	NN
cord-328460-thx9zh11	211	24	of	of	IN
cord-328460-thx9zh11	211	25	the	the	DT
cord-328460-thx9zh11	211	26	template	template	NN
cord-328460-thx9zh11	211	27	material	material	NN
cord-328460-thx9zh11	211	28	with	with	IN
cord-328460-thx9zh11	211	29	the	the	DT
cord-328460-thx9zh11	211	30	strand	strand	NN
cord-328460-thx9zh11	211	31	-	-	HYPH
cord-328460-thx9zh11	211	32	displacement	displacement	NN
cord-328460-thx9zh11	211	33	DNA	dna	NN
cord-328460-thx9zh11	211	34	synthesis	synthesis	NN
cord-328460-thx9zh11	211	35	.	.	.
cord-328460-thx9zh11	212	1	The	the	DT
cord-328460-thx9zh11	212	2	method	method	NN
cord-328460-thx9zh11	212	3	,	,	,
cord-328460-thx9zh11	212	4	introduced	introduce	VBN
cord-328460-thx9zh11	212	5	by	by	IN
cord-328460-thx9zh11	212	6	Piepenburg	Piepenburg	NNP
cord-328460-thx9zh11	212	7	et	et	NNP
cord-328460-thx9zh11	212	8	al	al	NNP
cord-328460-thx9zh11	212	9	.	.	.
cord-328460-thx9zh11	213	1	in	in	IN
cord-328460-thx9zh11	213	2	2006	2006	CD
cord-328460-thx9zh11	213	3	in	in	IN
cord-328460-thx9zh11	213	4	combination	combination	NN
cord-328460-thx9zh11	213	5	with	with	IN
cord-328460-thx9zh11	213	6	a	a	DT
cord-328460-thx9zh11	213	7	novel	novel	JJ
cord-328460-thx9zh11	213	8	probe	probe	NN
cord-328460-thx9zh11	213	9	-	-	HYPH
cord-328460-thx9zh11	213	10	based	base	VBN
cord-328460-thx9zh11	213	11	detection	detection	NN
cord-328460-thx9zh11	213	12	approach	approach	NN
cord-328460-thx9zh11	213	13	[	[	-LRB-
cord-328460-thx9zh11	213	14	101	101	CD
cord-328460-thx9zh11	213	15	]	]	-RRB-
cord-328460-thx9zh11	213	16	,	,	,
cord-328460-thx9zh11	213	17	amplifies	amplify	VBZ
cord-328460-thx9zh11	213	18	DNA	dna	NN
cord-328460-thx9zh11	213	19	sequences	sequence	NNS
cord-328460-thx9zh11	213	20	by	by	IN
cord-328460-thx9zh11	213	21	using	use	VBG
cord-328460-thx9zh11	213	22	a	a	DT
cord-328460-thx9zh11	213	23	recombinase	recombinase	NN
cord-328460-thx9zh11	213	24	,	,	,
cord-328460-thx9zh11	213	25	DNA	dna	NN
cord-328460-thx9zh11	213	26	polymerase	polymerase	NN
cord-328460-thx9zh11	213	27	and	and	CC
cord-328460-thx9zh11	213	28	DNA	dna	NN
cord-328460-thx9zh11	213	29	-	-	HYPH
cord-328460-thx9zh11	213	30	binding	bind	VBG
cord-328460-thx9zh11	213	31	proteins	protein	NNS
cord-328460-thx9zh11	213	32	.	.	.
cord-328460-thx9zh11	214	1	RPA	RPA	NNP
cord-328460-thx9zh11	214	2	uses	use	VBZ
cord-328460-thx9zh11	214	3	nucleoprotein	nucleoprotein	NNP
cord-328460-thx9zh11	214	4	complexes	complex	NNS
cord-328460-thx9zh11	214	5	constituted	constitute	VBN
cord-328460-thx9zh11	214	6	by	by	IN
cord-328460-thx9zh11	214	7	oligonucleotide	oligonucleotide	JJ
cord-328460-thx9zh11	214	8	primers	primer	NNS
cord-328460-thx9zh11	214	9	and	and	CC
cord-328460-thx9zh11	214	10	recombinase	recombinase	NN
cord-328460-thx9zh11	214	11	proteins	protein	NNS
cord-328460-thx9zh11	214	12	to	to	TO
cord-328460-thx9zh11	214	13	facilitate	facilitate	VB
cord-328460-thx9zh11	214	14	the	the	DT
cord-328460-thx9zh11	214	15	primer	primer	NN
cord-328460-thx9zh11	214	16	binding	bind	VBG
cord-328460-thx9zh11	214	17	to	to	IN
cord-328460-thx9zh11	214	18	the	the	DT
cord-328460-thx9zh11	214	19	template	template	NN
cord-328460-thx9zh11	214	20	DNA	dna	NN
cord-328460-thx9zh11	214	21	.	.	.
cord-328460-thx9zh11	215	1	In	in	IN
cord-328460-thx9zh11	215	2	particular	particular	JJ
cord-328460-thx9zh11	215	3	,	,	,
cord-328460-thx9zh11	215	4	recombinase	recombinase	NN
cord-328460-thx9zh11	215	5	-	-	HYPH
cord-328460-thx9zh11	215	6	primer	primer	NN
cord-328460-thx9zh11	215	7	complexes	complex	NNS
cord-328460-thx9zh11	215	8	scan	scan	VBP
cord-328460-thx9zh11	215	9	the	the	DT
cord-328460-thx9zh11	215	10	double	double	RB
cord-328460-thx9zh11	215	11	-	-	HYPH
cord-328460-thx9zh11	215	12	stranded	strand	VBN
cord-328460-thx9zh11	215	13	DNA	dna	NN
cord-328460-thx9zh11	215	14	by	by	IN
cord-328460-thx9zh11	215	15	promoting	promote	VBG
cord-328460-thx9zh11	215	16	the	the	DT
cord-328460-thx9zh11	215	17	primer	primer	NN
cord-328460-thx9zh11	215	18	binding	bind	VBG
cord-328460-thx9zh11	215	19	at	at	IN
cord-328460-thx9zh11	215	20	the	the	DT
cord-328460-thx9zh11	215	21	target	target	NN
cord-328460-thx9zh11	215	22	sequence	sequence	NN
cord-328460-thx9zh11	215	23	of	of	IN
cord-328460-thx9zh11	215	24	dsDNA	dsdna	NN
cord-328460-thx9zh11	215	25	and	and	CC
cord-328460-thx9zh11	215	26	the	the	DT
cord-328460-thx9zh11	215	27	displacement	displacement	NN
cord-328460-thx9zh11	215	28	of	of	IN
cord-328460-thx9zh11	215	29	the	the	DT
cord-328460-thx9zh11	215	30	non	non	JJ
cord-328460-thx9zh11	215	31	-	-	JJ
cord-328460-thx9zh11	215	32	template	template	JJ
cord-328460-thx9zh11	215	33	strand	strand	NN
cord-328460-thx9zh11	215	34	.	.	.
cord-328460-thx9zh11	216	1	The	the	DT
cord-328460-thx9zh11	216	2	displaced	displace	VBN
cord-328460-thx9zh11	216	3	strand	strand	NN
cord-328460-thx9zh11	216	4	is	be	VBZ
cord-328460-thx9zh11	216	5	stabilized	stabilize	VBN
cord-328460-thx9zh11	216	6	by	by	IN
cord-328460-thx9zh11	216	7	ssDNA	ssdna	NN
cord-328460-thx9zh11	216	8	binding	bind	VBG
cord-328460-thx9zh11	216	9	proteins	protein	NNS
cord-328460-thx9zh11	216	10	while	while	IN
cord-328460-thx9zh11	216	11	the	the	DT
cord-328460-thx9zh11	216	12	recombinase	recombinase	NNP
cord-328460-thx9zh11	216	13	disassembly	disassembly	NN
cord-328460-thx9zh11	216	14	leaves	leave	VBZ
cord-328460-thx9zh11	216	15	3'-end	3'-end	CD
cord-328460-thx9zh11	216	16	of	of	IN
cord-328460-thx9zh11	216	17	the	the	DT
cord-328460-thx9zh11	216	18	primer	primer	NN
cord-328460-thx9zh11	216	19	accessible	accessible	JJ
cord-328460-thx9zh11	216	20	to	to	IN
cord-328460-thx9zh11	216	21	DNA	dna	NN
cord-328460-thx9zh11	216	22	polymerase	polymerase	NN
cord-328460-thx9zh11	216	23	.	.	.
cord-328460-thx9zh11	217	1	An	an	DT
cord-328460-thx9zh11	217	2	exponential	exponential	JJ
cord-328460-thx9zh11	217	3	amplification	amplification	NN
cord-328460-thx9zh11	217	4	of	of	IN
cord-328460-thx9zh11	217	5	the	the	DT
cord-328460-thx9zh11	217	6	target	target	NN
cord-328460-thx9zh11	217	7	sequence	sequence	NN
cord-328460-thx9zh11	217	8	is	be	VBZ
cord-328460-thx9zh11	217	9	obtained	obtain	VBN
cord-328460-thx9zh11	217	10	after	after	IN
cord-328460-thx9zh11	217	11	the	the	DT
cord-328460-thx9zh11	217	12	cyclic	cyclic	JJ
cord-328460-thx9zh11	217	13	replication	replication	NN
cord-328460-thx9zh11	217	14	of	of	IN
cord-328460-thx9zh11	217	15	the	the	DT
cord-328460-thx9zh11	217	16	process	process	NN
cord-328460-thx9zh11	217	17	.	.	.
cord-328460-thx9zh11	218	1	RPA	RPA	NNP
cord-328460-thx9zh11	218	2	isothermal	isothermal	JJ
cord-328460-thx9zh11	218	3	amplification	amplification	NN
cord-328460-thx9zh11	218	4	has	have	VBZ
cord-328460-thx9zh11	218	5	been	be	VBN
cord-328460-thx9zh11	218	6	integrated	integrate	VBN
cord-328460-thx9zh11	218	7	in	in	IN
cord-328460-thx9zh11	218	8	a	a	DT
cord-328460-thx9zh11	218	9	centrifugal	centrifugal	JJ
cord-328460-thx9zh11	218	10	lab	lab	NN
cord-328460-thx9zh11	218	11	-	-	HYPH
cord-328460-thx9zh11	218	12	on	on	IN
cord-328460-thx9zh11	218	13	-	-	HYPH
cord-328460-thx9zh11	218	14	a	a	DT
cord-328460-thx9zh11	218	15	-	-	HYPH
cord-328460-thx9zh11	218	16	chip	chip	NN
cord-328460-thx9zh11	218	17	device	device	NN
cord-328460-thx9zh11	218	18	[	[	-LRB-
cord-328460-thx9zh11	218	19	102	102	CD
cord-328460-thx9zh11	218	20	]	]	-RRB-
cord-328460-thx9zh11	218	21	.	.	.
cord-328460-thx9zh11	219	1	The	the	DT
cord-328460-thx9zh11	219	2	fluidic	fluidic	JJ
cord-328460-thx9zh11	219	3	cartridge	cartridge	NN
cord-328460-thx9zh11	219	4	of	of	IN
cord-328460-thx9zh11	219	5	the	the	DT
cord-328460-thx9zh11	219	6	device	device	NN
cord-328460-thx9zh11	219	7	was	be	VBD
cord-328460-thx9zh11	219	8	fabricated	fabricate	VBN
cord-328460-thx9zh11	219	9	with	with	IN
cord-328460-thx9zh11	219	10	a	a	DT
cord-328460-thx9zh11	219	11	thermoforming	thermoforming	NN
cord-328460-thx9zh11	219	12	process	process	NN
cord-328460-thx9zh11	219	13	and	and	CC
cord-328460-thx9zh11	219	14	included	include	VBD
cord-328460-thx9zh11	219	15	fluidic	fluidic	JJ
cord-328460-thx9zh11	219	16	cavities	cavity	NNS
cord-328460-thx9zh11	219	17	for	for	IN
cord-328460-thx9zh11	219	18	the	the	DT
cord-328460-thx9zh11	219	19	processing	processing	NN
cord-328460-thx9zh11	219	20	of	of	IN
cord-328460-thx9zh11	219	21	up	up	IN
cord-328460-thx9zh11	219	22	to	to	TO
cord-328460-thx9zh11	219	23	30	30	CD
cord-328460-thx9zh11	219	24	samples	sample	NNS
cord-328460-thx9zh11	219	25	in	in	IN
cord-328460-thx9zh11	219	26	parallel	parallel	NN
cord-328460-thx9zh11	219	27	in	in	IN
cord-328460-thx9zh11	219	28	separate	separate	JJ
cord-328460-thx9zh11	219	29	10	10	CD
cord-328460-thx9zh11	219	30	µL	µl	IN
cord-328460-thx9zh11	219	31	microchambers	microchamber	NNS
cord-328460-thx9zh11	219	32	.	.	.
cord-328460-thx9zh11	220	1	Liquid	liquid	JJ
cord-328460-thx9zh11	220	2	and	and	CC
cord-328460-thx9zh11	220	3	dry	dry	JJ
cord-328460-thx9zh11	220	4	reagents	reagent	NNS
cord-328460-thx9zh11	220	5	for	for	IN
cord-328460-thx9zh11	220	6	the	the	DT
cord-328460-thx9zh11	220	7	amplification	amplification	NN
cord-328460-thx9zh11	220	8	were	be	VBD
cord-328460-thx9zh11	220	9	pre	pre	VBN
cord-328460-thx9zh11	220	10	-	-	VBN
cord-328460-thx9zh11	220	11	stored	store	VBN
cord-328460-thx9zh11	220	12	on	on	IN
cord-328460-thx9zh11	220	13	the	the	DT
cord-328460-thx9zh11	220	14	chip	chip	NN
cord-328460-thx9zh11	220	15	while	while	IN
cord-328460-thx9zh11	220	16	the	the	DT
cord-328460-thx9zh11	220	17	DNA	dna	NN
cord-328460-thx9zh11	220	18	sample	sample	NN
cord-328460-thx9zh11	220	19	was	be	VBD
cord-328460-thx9zh11	220	20	directly	directly	RB
cord-328460-thx9zh11	220	21	added	add	VBN
cord-328460-thx9zh11	220	22	to	to	IN
cord-328460-thx9zh11	220	23	each	each	DT
cord-328460-thx9zh11	220	24	microchamber	microchamber	NN
cord-328460-thx9zh11	220	25	in	in	IN
cord-328460-thx9zh11	220	26	order	order	NN
cord-328460-thx9zh11	220	27	to	to	TO
cord-328460-thx9zh11	220	28	minimize	minimize	VB
cord-328460-thx9zh11	220	29	the	the	DT
cord-328460-thx9zh11	220	30	influence	influence	NN
cord-328460-thx9zh11	220	31	of	of	IN
cord-328460-thx9zh11	220	32	surface	surface	NN
cord-328460-thx9zh11	220	33	adsorption	adsorption	NN
cord-328460-thx9zh11	220	34	effects	effect	NNS
cord-328460-thx9zh11	220	35	.	.	.
cord-328460-thx9zh11	221	1	Spinning	spin	VBG
cord-328460-thx9zh11	221	2	was	be	VBD
cord-328460-thx9zh11	221	3	used	use	VBN
cord-328460-thx9zh11	221	4	to	to	TO
cord-328460-thx9zh11	221	5	transfer	transfer	VB
cord-328460-thx9zh11	221	6	the	the	DT
cord-328460-thx9zh11	221	7	liquid	liquid	NN
cord-328460-thx9zh11	221	8	to	to	IN
cord-328460-thx9zh11	221	9	the	the	DT
cord-328460-thx9zh11	221	10	chamber	chamber	NN
cord-328460-thx9zh11	221	11	containing	contain	VBG
cord-328460-thx9zh11	221	12	the	the	DT
cord-328460-thx9zh11	221	13	lyophilized	lyophilized	JJ
cord-328460-thx9zh11	221	14	RPA	RPA	NNP
cord-328460-thx9zh11	221	15	mixture	mixture	NN
cord-328460-thx9zh11	221	16	and	and	CC
cord-328460-thx9zh11	221	17	to	to	TO
cord-328460-thx9zh11	221	18	move	move	VB
cord-328460-thx9zh11	221	19	fluids	fluid	NNS
cord-328460-thx9zh11	221	20	between	between	IN
cord-328460-thx9zh11	221	21	chambers	chamber	NNS
cord-328460-thx9zh11	221	22	.	.	.
cord-328460-thx9zh11	222	1	Hakenberg	Hakenberg	NNP
cord-328460-thx9zh11	222	2	et	et	NNP
cord-328460-thx9zh11	222	3	al	al	NNP
cord-328460-thx9zh11	222	4	.	.	NNP
cord-328460-thx9zh11	222	5	developed	develop	VBD
cord-328460-thx9zh11	222	6	a	a	DT
cord-328460-thx9zh11	222	7	passive	passive	JJ
cord-328460-thx9zh11	222	8	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	222	9	batch	batch	NN
cord-328460-thx9zh11	222	10	mixing	mix	VBG
cord-328460-thx9zh11	222	11	chip	chip	NN
cord-328460-thx9zh11	222	12	for	for	IN
cord-328460-thx9zh11	222	13	RPA	RPA	NNP
cord-328460-thx9zh11	222	14	fabricated	fabricate	VBN
cord-328460-thx9zh11	222	15	through	through	IN
cord-328460-thx9zh11	222	16	a	a	DT
cord-328460-thx9zh11	222	17	simple	simple	JJ
cord-328460-thx9zh11	222	18	and	and	CC
cord-328460-thx9zh11	222	19	low	low	JJ
cord-328460-thx9zh11	222	20	-	-	HYPH
cord-328460-thx9zh11	222	21	cost	cost	NN
cord-328460-thx9zh11	222	22	method	method	NN
cord-328460-thx9zh11	222	23	which	which	WDT
cord-328460-thx9zh11	222	24	combines	combine	VBZ
cord-328460-thx9zh11	222	25	dry	dry	JJ
cord-328460-thx9zh11	222	26	film	film	NN
cord-328460-thx9zh11	222	27	resist	resist	VB
cord-328460-thx9zh11	222	28	technology	technology	NN
cord-328460-thx9zh11	222	29	and	and	CC
cord-328460-thx9zh11	222	30	direct	direct	JJ
cord-328460-thx9zh11	222	31	wafer	wafer	NN
cord-328460-thx9zh11	222	32	bonding	bonding	NN
cord-328460-thx9zh11	222	33	[	[	-LRB-
cord-328460-thx9zh11	222	34	103	103	CD
cord-328460-thx9zh11	222	35	]	]	-RRB-
cord-328460-thx9zh11	222	36	.	.	.
cord-328460-thx9zh11	223	1	In	in	IN
cord-328460-thx9zh11	223	2	this	this	DT
cord-328460-thx9zh11	223	3	detection	detection	NN
cord-328460-thx9zh11	223	4	assay	assay	NN
cord-328460-thx9zh11	223	5	,	,	,
cord-328460-thx9zh11	223	6	based	base	VBN
cord-328460-thx9zh11	223	7	on	on	IN
cord-328460-thx9zh11	223	8	the	the	DT
cord-328460-thx9zh11	223	9	phaseguided	phaseguided	JJ
cord-328460-thx9zh11	223	10	fluid	fluid	NN
cord-328460-thx9zh11	223	11	handling	handling	NN
cord-328460-thx9zh11	223	12	[	[	-LRB-
cord-328460-thx9zh11	223	13	104	104	CD
cord-328460-thx9zh11	223	14	]	]	-RRB-
cord-328460-thx9zh11	223	15	,	,	,
cord-328460-thx9zh11	223	16	fluorescence	fluorescence	NN
cord-328460-thx9zh11	223	17	results	result	NNS
cord-328460-thx9zh11	223	18	are	be	VBP
cord-328460-thx9zh11	223	19	measured	measure	VBN
cord-328460-thx9zh11	223	20	directly	directly	RB
cord-328460-thx9zh11	223	21	from	from	IN
cord-328460-thx9zh11	223	22	the	the	DT
cord-328460-thx9zh11	223	23	chip	chip	NN
cord-328460-thx9zh11	223	24	after	after	IN
cord-328460-thx9zh11	223	25	a	a	DT
cord-328460-thx9zh11	223	26	one	one	CD
cord-328460-thx9zh11	223	27	minute	minute	NN
cord-328460-thx9zh11	223	28	mixing	mix	VBG
cord-328460-thx9zh11	223	29	sequence	sequence	NN
cord-328460-thx9zh11	223	30	.	.	.
cord-328460-thx9zh11	224	1	Digital	Digital	NNP
cord-328460-thx9zh11	224	2	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	224	3	isothermal	isothermal	JJ
cord-328460-thx9zh11	224	4	amplification	amplification	NN
cord-328460-thx9zh11	224	5	methods	method	NNS
cord-328460-thx9zh11	224	6	that	that	WDT
cord-328460-thx9zh11	224	7	take	take	VBP
cord-328460-thx9zh11	224	8	place	place	NN
cord-328460-thx9zh11	224	9	at	at	IN
cord-328460-thx9zh11	224	10	temperatures	temperature	NNS
cord-328460-thx9zh11	224	11	close	close	RB
cord-328460-thx9zh11	224	12	to	to	IN
cord-328460-thx9zh11	224	13	the	the	DT
cord-328460-thx9zh11	224	14	room	room	NN
cord-328460-thx9zh11	224	15	temperature	temperature	NN
cord-328460-thx9zh11	224	16	are	be	VBP
cord-328460-thx9zh11	224	17	convenient	convenient	JJ
cord-328460-thx9zh11	224	18	because	because	IN
cord-328460-thx9zh11	224	19	the	the	DT
cord-328460-thx9zh11	224	20	polymerization	polymerization	NN
cord-328460-thx9zh11	224	21	reaction	reaction	NN
cord-328460-thx9zh11	224	22	is	be	VBZ
cord-328460-thx9zh11	224	23	chemistry	chemistry	NN
cord-328460-thx9zh11	224	24	rather	rather	RB
cord-328460-thx9zh11	224	25	that	that	IN
cord-328460-thx9zh11	224	26	temperature	temperature	NN
cord-328460-thx9zh11	224	27	actuated	actuate	VBN
cord-328460-thx9zh11	224	28	.	.	.
cord-328460-thx9zh11	225	1	However	however	RB
cord-328460-thx9zh11	225	2	,	,	,
cord-328460-thx9zh11	225	3	these	these	DT
cord-328460-thx9zh11	225	4	methods	method	NNS
cord-328460-thx9zh11	225	5	suffer	suffer	VBP
cord-328460-thx9zh11	225	6	from	from	IN
cord-328460-thx9zh11	225	7	the	the	DT
cord-328460-thx9zh11	225	8	unwanted	unwanted	JJ
cord-328460-thx9zh11	225	9	characteristic	characteristic	NN
cord-328460-thx9zh11	225	10	that	that	IN
cord-328460-thx9zh11	225	11	the	the	DT
cord-328460-thx9zh11	225	12	amplification	amplification	NN
cord-328460-thx9zh11	225	13	reaction	reaction	NN
cord-328460-thx9zh11	225	14	is	be	VBZ
cord-328460-thx9zh11	225	15	expected	expect	VBN
cord-328460-thx9zh11	225	16	to	to	TO
cord-328460-thx9zh11	225	17	proceed	proceed	VB
cord-328460-thx9zh11	225	18	at	at	IN
cord-328460-thx9zh11	225	19	room	room	NN
cord-328460-thx9zh11	225	20	temperature	temperature	NN
cord-328460-thx9zh11	225	21	if	if	IN
cord-328460-thx9zh11	225	22	the	the	DT
cord-328460-thx9zh11	225	23	nucleic	nucleic	JJ
cord-328460-thx9zh11	225	24	acid	acid	NN
cord-328460-thx9zh11	225	25	sample	sample	NN
cord-328460-thx9zh11	225	26	is	be	VBZ
cord-328460-thx9zh11	225	27	premixed	premixe	VBN
cord-328460-thx9zh11	225	28	with	with	IN
cord-328460-thx9zh11	225	29	initiation	initiation	NN
cord-328460-thx9zh11	225	30	reagents	reagent	NNS
cord-328460-thx9zh11	225	31	prior	prior	RB
cord-328460-thx9zh11	225	32	to	to	IN
cord-328460-thx9zh11	225	33	compartmentalization	compartmentalization	NN
cord-328460-thx9zh11	225	34	.	.	.
cord-328460-thx9zh11	226	1	Conversely	conversely	RB
cord-328460-thx9zh11	226	2	,	,	,
cord-328460-thx9zh11	226	3	in	in	IN
cord-328460-thx9zh11	226	4	digital	digital	JJ
cord-328460-thx9zh11	226	5	PCR	PCR	NNP
cord-328460-thx9zh11	226	6	the	the	DT
cord-328460-thx9zh11	226	7	amplification	amplification	NN
cord-328460-thx9zh11	226	8	reaction	reaction	NN
cord-328460-thx9zh11	226	9	is	be	VBZ
cord-328460-thx9zh11	226	10	controlled	control	VBN
cord-328460-thx9zh11	226	11	by	by	IN
cord-328460-thx9zh11	226	12	temperature	temperature	NN
cord-328460-thx9zh11	226	13	and	and	CC
cord-328460-thx9zh11	226	14	any	any	DT
cord-328460-thx9zh11	226	15	low	low	JJ
cord-328460-thx9zh11	226	16	-	-	HYPH
cord-328460-thx9zh11	226	17	temperature	temperature	NN
cord-328460-thx9zh11	226	18	non	non	JJ
cord-328460-thx9zh11	226	19	-	-	JJ
cord-328460-thx9zh11	226	20	specific	specific	JJ
cord-328460-thx9zh11	226	21	pre	pre	JJ
cord-328460-thx9zh11	226	22	-	-	JJ
cord-328460-thx9zh11	226	23	amplification	amplification	JJ
cord-328460-thx9zh11	226	24	process	process	NN
cord-328460-thx9zh11	226	25	can	can	MD
cord-328460-thx9zh11	226	26	be	be	VB
cord-328460-thx9zh11	226	27	eliminated	eliminate	VBN
cord-328460-thx9zh11	226	28	by	by	IN
cord-328460-thx9zh11	226	29	using	use	VBG
cord-328460-thx9zh11	226	30	specific	specific	JJ
cord-328460-thx9zh11	226	31	procedures	procedure	NNS
cord-328460-thx9zh11	226	32	[	[	-LRB-
cord-328460-thx9zh11	226	33	105	105	CD
cord-328460-thx9zh11	226	34	]	]	-RRB-
cord-328460-thx9zh11	226	35	.	.	.
cord-328460-thx9zh11	227	1	As	as	IN
cord-328460-thx9zh11	227	2	a	a	DT
cord-328460-thx9zh11	227	3	consequence	consequence	NN
cord-328460-thx9zh11	227	4	of	of	IN
cord-328460-thx9zh11	227	5	that	that	DT
cord-328460-thx9zh11	227	6	,	,	,
cord-328460-thx9zh11	227	7	the	the	DT
cord-328460-thx9zh11	227	8	mixture	mixture	NN
cord-328460-thx9zh11	227	9	can	can	MD
cord-328460-thx9zh11	227	10	be	be	VB
cord-328460-thx9zh11	227	11	compartmentalized	compartmentalize	VBN
cord-328460-thx9zh11	227	12	prior	prior	RB
cord-328460-thx9zh11	227	13	to	to	IN
cord-328460-thx9zh11	227	14	initiation	initiation	NN
cord-328460-thx9zh11	227	15	with	with	IN
cord-328460-thx9zh11	227	16	minimal	minimal	JJ
cord-328460-thx9zh11	227	17	risk	risk	NN
cord-328460-thx9zh11	227	18	of	of	IN
cord-328460-thx9zh11	227	19	false	false	JJ
cord-328460-thx9zh11	227	20	positives	positive	NNS
cord-328460-thx9zh11	227	21	if	if	IN
cord-328460-thx9zh11	227	22	the	the	DT
cord-328460-thx9zh11	227	23	infrastructure	infrastructure	NN
cord-328460-thx9zh11	227	24	for	for	IN
cord-328460-thx9zh11	227	25	an	an	DT
cord-328460-thx9zh11	227	26	accurate	accurate	JJ
cord-328460-thx9zh11	227	27	temperature	temperature	NN
cord-328460-thx9zh11	227	28	control	control	NN
cord-328460-thx9zh11	227	29	is	be	VBZ
cord-328460-thx9zh11	227	30	readily	readily	RB
cord-328460-thx9zh11	227	31	available	available	JJ
cord-328460-thx9zh11	227	32	.	.	.
cord-328460-thx9zh11	228	1	In	in	IN
cord-328460-thx9zh11	228	2	point	point	NN
cord-328460-thx9zh11	228	3	-	-	HYPH
cord-328460-thx9zh11	228	4	of	of	IN
cord-328460-thx9zh11	228	5	-	-	HYPH
cord-328460-thx9zh11	228	6	care	care	NN
cord-328460-thx9zh11	228	7	settings	setting	NNS
cord-328460-thx9zh11	228	8	,	,	,
cord-328460-thx9zh11	228	9	a	a	DT
cord-328460-thx9zh11	228	10	digital	digital	JJ
cord-328460-thx9zh11	228	11	isothermal	isothermal	JJ
cord-328460-thx9zh11	228	12	nucleic	nucleic	JJ
cord-328460-thx9zh11	228	13	acid	acid	NN
cord-328460-thx9zh11	228	14	amplification	amplification	NN
cord-328460-thx9zh11	228	15	approach	approach	NN
cord-328460-thx9zh11	228	16	like	like	IN
cord-328460-thx9zh11	228	17	RPA	RPA	NNP
cord-328460-thx9zh11	228	18	represents	represent	VBZ
cord-328460-thx9zh11	228	19	an	an	DT
cord-328460-thx9zh11	228	20	attractive	attractive	JJ
cord-328460-thx9zh11	228	21	option	option	NN
cord-328460-thx9zh11	228	22	for	for	IN
cord-328460-thx9zh11	228	23	nucleic	nucleic	JJ
cord-328460-thx9zh11	228	24	acid	acid	NN
cord-328460-thx9zh11	228	25	quantification	quantification	NN
cord-328460-thx9zh11	228	26	but	but	CC
cord-328460-thx9zh11	228	27	,	,	,
cord-328460-thx9zh11	228	28	in	in	IN
cord-328460-thx9zh11	228	29	order	order	NN
cord-328460-thx9zh11	228	30	to	to	TO
cord-328460-thx9zh11	228	31	achieve	achieve	VB
cord-328460-thx9zh11	228	32	digital	digital	JJ
cord-328460-thx9zh11	228	33	RPA	RPA	NNP
cord-328460-thx9zh11	228	34	without	without	IN
cord-328460-thx9zh11	228	35	false	false	JJ
cord-328460-thx9zh11	228	36	-	-	HYPH
cord-328460-thx9zh11	228	37	positive	positive	JJ
cord-328460-thx9zh11	228	38	errors	error	NNS
cord-328460-thx9zh11	228	39	,	,	,
cord-328460-thx9zh11	228	40	the	the	DT
cord-328460-thx9zh11	228	41	nucleic	nucleic	JJ
cord-328460-thx9zh11	228	42	acid	acid	NN
cord-328460-thx9zh11	228	43	template	template	NN
cord-328460-thx9zh11	228	44	must	must	MD
cord-328460-thx9zh11	228	45	be	be	VB
cord-328460-thx9zh11	228	46	compartmentalized	compartmentalize	VBN
cord-328460-thx9zh11	228	47	prior	prior	RB
cord-328460-thx9zh11	228	48	to	to	IN
cord-328460-thx9zh11	228	49	adding	add	VBG
cord-328460-thx9zh11	228	50	initiation	initiation	NN
cord-328460-thx9zh11	228	51	reagents	reagent	NNS
cord-328460-thx9zh11	228	52	.	.	.
cord-328460-thx9zh11	229	1	RPA	RPA	NNP
cord-328460-thx9zh11	229	2	has	have	VBZ
cord-328460-thx9zh11	229	3	been	be	VBN
cord-328460-thx9zh11	229	4	implemented	implement	VBN
cord-328460-thx9zh11	229	5	in	in	IN
cord-328460-thx9zh11	229	6	a	a	DT
cord-328460-thx9zh11	229	7	droplet	droplet	NN
cord-328460-thx9zh11	229	8	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	229	9	chip	chip	NN
cord-328460-thx9zh11	229	10	for	for	IN
cord-328460-thx9zh11	229	11	the	the	DT
cord-328460-thx9zh11	229	12	simultaneous	simultaneous	JJ
cord-328460-thx9zh11	229	13	chemical	chemical	NN
cord-328460-thx9zh11	229	14	initiation	initiation	NN
cord-328460-thx9zh11	229	15	of	of	IN
cord-328460-thx9zh11	229	16	over	over	IN
cord-328460-thx9zh11	229	17	one	one	CD
cord-328460-thx9zh11	229	18	thousand	thousand	CD
cord-328460-thx9zh11	229	19	sequence	sequence	NN
cord-328460-thx9zh11	229	20	-	-	HYPH
cord-328460-thx9zh11	229	21	specific	specific	JJ
cord-328460-thx9zh11	229	22	RPA	RPA	NNP
cord-328460-thx9zh11	229	23	in	in	IN
cord-328460-thx9zh11	229	24	parallel	parallel	NN
cord-328460-thx9zh11	229	25	[	[	-LRB-
cord-328460-thx9zh11	229	26	106	106	CD
cord-328460-thx9zh11	229	27	]	]	-RRB-
cord-328460-thx9zh11	229	28	.	.	.
cord-328460-thx9zh11	230	1	The	the	DT
cord-328460-thx9zh11	230	2	amplification	amplification	NN
cord-328460-thx9zh11	230	3	process	process	NN
cord-328460-thx9zh11	230	4	was	be	VBD
cord-328460-thx9zh11	230	5	obtained	obtain	VBN
cord-328460-thx9zh11	230	6	by	by	IN
cord-328460-thx9zh11	230	7	adding	add	VBG
cord-328460-thx9zh11	230	8	a	a	DT
cord-328460-thx9zh11	230	9	chemical	chemical	NN
cord-328460-thx9zh11	230	10	initiator	initiator	NN
cord-328460-thx9zh11	230	11	to	to	IN
cord-328460-thx9zh11	230	12	each	each	DT
cord-328460-thx9zh11	230	13	reaction	reaction	NN
cord-328460-thx9zh11	230	14	compartment	compartment	NN
cord-328460-thx9zh11	230	15	with	with	IN
cord-328460-thx9zh11	230	16	a	a	DT
cord-328460-thx9zh11	230	17	simple	simple	JJ
cord-328460-thx9zh11	230	18	slipping	slip	VBG
cord-328460-thx9zh11	230	19	step	step	NN
cord-328460-thx9zh11	230	20	after	after	IN
cord-328460-thx9zh11	230	21	instrument	instrument	NN
cord-328460-thx9zh11	230	22	-	-	HYPH
cord-328460-thx9zh11	230	23	free	free	JJ
cord-328460-thx9zh11	230	24	pipet	pipet	NN
cord-328460-thx9zh11	230	25	loading	loading	NN
cord-328460-thx9zh11	230	26	.	.	.
cord-328460-thx9zh11	231	1	The	the	DT
cord-328460-thx9zh11	231	2	digital	digital	JJ
cord-328460-thx9zh11	231	3	RPA	RPA	NNP
cord-328460-thx9zh11	231	4	did	do	VBD
cord-328460-thx9zh11	231	5	not	not	RB
cord-328460-thx9zh11	231	6	require	require	VB
cord-328460-thx9zh11	231	7	precise	precise	JJ
cord-328460-thx9zh11	231	8	temperature	temperature	NN
cord-328460-thx9zh11	231	9	control	control	NN
cord-328460-thx9zh11	231	10	since	since	IN
cord-328460-thx9zh11	231	11	it	-PRON-	PRP
cord-328460-thx9zh11	231	12	is	be	VBZ
cord-328460-thx9zh11	231	13	tolerant	tolerant	JJ
cord-328460-thx9zh11	231	14	to	to	IN
cord-328460-thx9zh11	231	15	fluctuations	fluctuation	NNS
cord-328460-thx9zh11	231	16	of	of	IN
cord-328460-thx9zh11	231	17	the	the	DT
cord-328460-thx9zh11	231	18	incubation	incubation	NN
cord-328460-thx9zh11	231	19	temperature	temperature	NN
cord-328460-thx9zh11	231	20	ranging	range	VBG
cord-328460-thx9zh11	231	21	from	from	IN
cord-328460-thx9zh11	231	22	37	37	CD
cord-328460-thx9zh11	231	23	°	°	NN
cord-328460-thx9zh11	231	24	C	c	NN
cord-328460-thx9zh11	231	25	to	to	IN
cord-328460-thx9zh11	231	26	42	42	CD
cord-328460-thx9zh11	231	27	°	°	NN
cord-328460-thx9zh11	231	28	C	c	NN
cord-328460-thx9zh11	231	29	.	.	.
cord-328460-thx9zh11	232	1	The	the	DT
cord-328460-thx9zh11	232	2	generation	generation	NN
cord-328460-thx9zh11	232	3	of	of	IN
cord-328460-thx9zh11	232	4	amplified	amplify	VBN
cord-328460-thx9zh11	232	5	target	target	NN
cord-328460-thx9zh11	232	6	material	material	NN
cord-328460-thx9zh11	232	7	was	be	VBD
cord-328460-thx9zh11	232	8	monitored	monitor	VBN
cord-328460-thx9zh11	232	9	by	by	IN
cord-328460-thx9zh11	232	10	fluorescence	fluorescence	NN
cord-328460-thx9zh11	232	11	by	by	IN
cord-328460-thx9zh11	232	12	using	use	VBG
cord-328460-thx9zh11	232	13	a	a	DT
cord-328460-thx9zh11	232	14	system	system	NN
cord-328460-thx9zh11	232	15	in	in	IN
cord-328460-thx9zh11	232	16	which	which	WDT
cord-328460-thx9zh11	232	17	a	a	DT
cord-328460-thx9zh11	232	18	fluorophore	fluorophore	NN
cord-328460-thx9zh11	232	19	/	/	SYM
cord-328460-thx9zh11	232	20	quencher	quencher	NN
cord-328460-thx9zh11	232	21	bearing	bearing	NN
cord-328460-thx9zh11	232	22	probe	probe	NN
cord-328460-thx9zh11	232	23	was	be	VBD
cord-328460-thx9zh11	232	24	nucleolytically	nucleolytically	RB
cord-328460-thx9zh11	232	25	cut	cut	VBN
cord-328460-thx9zh11	232	26	in	in	IN
cord-328460-thx9zh11	232	27	response	response	NN
cord-328460-thx9zh11	232	28	to	to	IN
cord-328460-thx9zh11	232	29	sequence	sequence	NN
cord-328460-thx9zh11	232	30	-	-	HYPH
cord-328460-thx9zh11	232	31	specific	specific	JJ
cord-328460-thx9zh11	232	32	binding	binding	NN
cord-328460-thx9zh11	232	33	to	to	IN
cord-328460-thx9zh11	232	34	amplified	amplify	VBN
cord-328460-thx9zh11	232	35	DNA	DNA	NNP
cord-328460-thx9zh11	232	36	leading	lead	VBG
cord-328460-thx9zh11	232	37	to	to	IN
cord-328460-thx9zh11	232	38	an	an	DT
cord-328460-thx9zh11	232	39	increase	increase	NN
cord-328460-thx9zh11	232	40	in	in	IN
cord-328460-thx9zh11	232	41	observable	observable	JJ
cord-328460-thx9zh11	232	42	fluorescence	fluorescence	NN
cord-328460-thx9zh11	232	43	as	as	IN
cord-328460-thx9zh11	232	44	a	a	DT
cord-328460-thx9zh11	232	45	result	result	NN
cord-328460-thx9zh11	232	46	of	of	IN
cord-328460-thx9zh11	232	47	separation	separation	NN
cord-328460-thx9zh11	232	48	of	of	IN
cord-328460-thx9zh11	232	49	the	the	DT
cord-328460-thx9zh11	232	50	fluorophore	fluorophore	NN
cord-328460-thx9zh11	232	51	and	and	CC
cord-328460-thx9zh11	232	52	quencher	quencher	NN
cord-328460-thx9zh11	232	53	groups	group	NNS
cord-328460-thx9zh11	232	54	.	.	.
cord-328460-thx9zh11	233	1	NASBA	NASBA	NNP
cord-328460-thx9zh11	233	2	is	be	VBZ
cord-328460-thx9zh11	233	3	an	an	DT
cord-328460-thx9zh11	233	4	isothermal	isothermal	JJ
cord-328460-thx9zh11	233	5	,	,	,
cord-328460-thx9zh11	233	6	transcription	transcription	NN
cord-328460-thx9zh11	233	7	-	-	HYPH
cord-328460-thx9zh11	233	8	based	base	VBN
cord-328460-thx9zh11	233	9	amplification	amplification	NN
cord-328460-thx9zh11	233	10	method	method	NN
cord-328460-thx9zh11	233	11	specifically	specifically	RB
cord-328460-thx9zh11	233	12	designed	design	VBN
cord-328460-thx9zh11	233	13	for	for	IN
cord-328460-thx9zh11	233	14	the	the	DT
cord-328460-thx9zh11	233	15	amplification	amplification	NN
cord-328460-thx9zh11	233	16	of	of	IN
cord-328460-thx9zh11	233	17	single	single	RB
cord-328460-thx9zh11	233	18	-	-	HYPH
cord-328460-thx9zh11	233	19	stranded	strand	VBN
cord-328460-thx9zh11	233	20	RNA	RNA	NNP
cord-328460-thx9zh11	233	21	or	or	CC
cord-328460-thx9zh11	233	22	DNA	dna	NN
cord-328460-thx9zh11	233	23	sequence	sequence	NN
cord-328460-thx9zh11	233	24	.	.	.
cord-328460-thx9zh11	234	1	It	-PRON-	PRP
cord-328460-thx9zh11	234	2	was	be	VBD
cord-328460-thx9zh11	234	3	first	first	RB
cord-328460-thx9zh11	234	4	introduced	introduce	VBN
cord-328460-thx9zh11	234	5	by	by	IN
cord-328460-thx9zh11	234	6	Compton	Compton	NNP
cord-328460-thx9zh11	234	7	in	in	IN
cord-328460-thx9zh11	234	8	1991	1991	CD
cord-328460-thx9zh11	234	9	[	[	-LRB-
cord-328460-thx9zh11	234	10	107	107	CD
cord-328460-thx9zh11	234	11	]	]	-RRB-
cord-328460-thx9zh11	234	12	and	and	CC
cord-328460-thx9zh11	234	13	is	be	VBZ
cord-328460-thx9zh11	234	14	performed	perform	VBN
cord-328460-thx9zh11	234	15	at	at	IN
cord-328460-thx9zh11	234	16	41	41	CD
cord-328460-thx9zh11	234	17	°	°	NN
cord-328460-thx9zh11	234	18	C	c	NN
cord-328460-thx9zh11	234	19	.	.	.
cord-328460-thx9zh11	235	1	Thanks	thank	NNS
cord-328460-thx9zh11	235	2	to	to	IN
cord-328460-thx9zh11	235	3	the	the	DT
cord-328460-thx9zh11	235	4	integration	integration	NN
cord-328460-thx9zh11	235	5	of	of	IN
cord-328460-thx9zh11	235	6	the	the	DT
cord-328460-thx9zh11	235	7	reverse	reverse	JJ
cord-328460-thx9zh11	235	8	transcription	transcription	NN
cord-328460-thx9zh11	235	9	activity	activity	NN
cord-328460-thx9zh11	235	10	into	into	IN
cord-328460-thx9zh11	235	11	the	the	DT
cord-328460-thx9zh11	235	12	amplification	amplification	NN
cord-328460-thx9zh11	235	13	process	process	NN
cord-328460-thx9zh11	235	14	,	,	,
cord-328460-thx9zh11	235	15	the	the	DT
cord-328460-thx9zh11	235	16	method	method	NN
cord-328460-thx9zh11	235	17	is	be	VBZ
cord-328460-thx9zh11	235	18	especially	especially	RB
cord-328460-thx9zh11	235	19	suited	suit	VBN
cord-328460-thx9zh11	235	20	for	for	IN
cord-328460-thx9zh11	235	21	RNAs	rna	NNS
cord-328460-thx9zh11	235	22	such	such	JJ
cord-328460-thx9zh11	235	23	as	as	IN
cord-328460-thx9zh11	235	24	mRNA	mRNA	NNP
cord-328460-thx9zh11	235	25	,	,	,
cord-328460-thx9zh11	235	26	rRNA	rRNA	NNP
cord-328460-thx9zh11	235	27	,	,	,
cord-328460-thx9zh11	235	28	tmRNA	tmrna	NN
cord-328460-thx9zh11	235	29	or	or	CC
cord-328460-thx9zh11	235	30	genomic	genomic	JJ
cord-328460-thx9zh11	235	31	RNA	RNA	NNP
cord-328460-thx9zh11	235	32	[	[	-LRB-
cord-328460-thx9zh11	235	33	108	108	CD
cord-328460-thx9zh11	235	34	]	]	-RRB-
cord-328460-thx9zh11	235	35	.	.	.
cord-328460-thx9zh11	236	1	However	however	RB
cord-328460-thx9zh11	236	2	,	,	,
cord-328460-thx9zh11	236	3	NASBA	NASBA	NNP
cord-328460-thx9zh11	236	4	can	can	MD
cord-328460-thx9zh11	236	5	not	not	RB
cord-328460-thx9zh11	236	6	amplify	amplify	VB
cord-328460-thx9zh11	236	7	double	double	RB
cord-328460-thx9zh11	236	8	-	-	HYPH
cord-328460-thx9zh11	236	9	stranded	strand	VBN
cord-328460-thx9zh11	236	10	DNAs	dna	NNS
cord-328460-thx9zh11	236	11	not	not	RB
cord-328460-thx9zh11	236	12	subjected	subject	VBN
cord-328460-thx9zh11	236	13	to	to	IN
cord-328460-thx9zh11	236	14	a	a	DT
cord-328460-thx9zh11	236	15	denaturation	denaturation	NN
cord-328460-thx9zh11	236	16	step	step	NN
cord-328460-thx9zh11	236	17	[	[	-LRB-
cord-328460-thx9zh11	236	18	109	109	CD
cord-328460-thx9zh11	236	19	]	]	-RRB-
cord-328460-thx9zh11	236	20	.	.	.
cord-328460-thx9zh11	237	1	NASBA	NASBA	NNP
cord-328460-thx9zh11	237	2	utilizes	utilize	VBZ
cord-328460-thx9zh11	237	3	two	two	CD
cord-328460-thx9zh11	237	4	RNA	RNA	NNP
cord-328460-thx9zh11	237	5	target	target	NN
cord-328460-thx9zh11	237	6	-	-	HYPH
cord-328460-thx9zh11	237	7	specific	specific	JJ
cord-328460-thx9zh11	237	8	primers	primer	NNS
cord-328460-thx9zh11	237	9	and	and	CC
cord-328460-thx9zh11	237	10	three	three	CD
cord-328460-thx9zh11	237	11	enzymes	enzyme	NNS
cord-328460-thx9zh11	237	12	(	(	-LRB-
cord-328460-thx9zh11	237	13	i.e.	i.e.	FW
cord-328460-thx9zh11	237	14	,	,	,
cord-328460-thx9zh11	237	15	avian	avian	JJ
cord-328460-thx9zh11	237	16	myeloblastosis	myeloblastosis	NN
cord-328460-thx9zh11	237	17	virus	virus	NN
cord-328460-thx9zh11	237	18	reverse	reverse	NN
cord-328460-thx9zh11	237	19	transcriptase	transcriptase	NN
cord-328460-thx9zh11	237	20	,	,	,
cord-328460-thx9zh11	237	21	T7	T7	NNP
cord-328460-thx9zh11	237	22	DNA	dna	NN
cord-328460-thx9zh11	237	23	-	-	HYPH
cord-328460-thx9zh11	237	24	dependent	dependent	JJ
cord-328460-thx9zh11	237	25	RNA	RNA	NNP
cord-328460-thx9zh11	237	26	polymerase	polymerase	NN
cord-328460-thx9zh11	237	27	(	(	-LRB-
cord-328460-thx9zh11	237	28	DdRp	DdRp	NNP
cord-328460-thx9zh11	237	29	)	)	-RRB-
cord-328460-thx9zh11	237	30	and	and	CC
cord-328460-thx9zh11	237	31	RNase	RNase	NNP
cord-328460-thx9zh11	237	32	H	H	NNP
cord-328460-thx9zh11	237	33	)	)	-RRB-
cord-328460-thx9zh11	237	34	.	.	.
cord-328460-thx9zh11	238	1	The	the	DT
cord-328460-thx9zh11	238	2	standard	standard	JJ
cord-328460-thx9zh11	238	3	NASBA	NASBA	NNP
cord-328460-thx9zh11	238	4	protocol	protocol	NN
cord-328460-thx9zh11	238	5	for	for	IN
cord-328460-thx9zh11	238	6	RNA	rna	NN
cord-328460-thx9zh11	238	7	amplification	amplification	NN
cord-328460-thx9zh11	238	8	(	(	-LRB-
cord-328460-thx9zh11	238	9	Figure	figure	NN
cord-328460-thx9zh11	238	10	4	4	CD
cord-328460-thx9zh11	238	11	)	)	-RRB-
cord-328460-thx9zh11	238	12	requires	require	VBZ
cord-328460-thx9zh11	238	13	a	a	DT
cord-328460-thx9zh11	238	14	65	65	CD
cord-328460-thx9zh11	238	15	°	°	NN
cord-328460-thx9zh11	238	16	C	C	NNP
cord-328460-thx9zh11	238	17	RNA	RNA	NNP
cord-328460-thx9zh11	238	18	incubation	incubation	NN
cord-328460-thx9zh11	238	19	step	step	NN
cord-328460-thx9zh11	238	20	to	to	TO
cord-328460-thx9zh11	238	21	denature	denature	VB
cord-328460-thx9zh11	238	22	the	the	DT
cord-328460-thx9zh11	238	23	target	target	NN
cord-328460-thx9zh11	238	24	prior	prior	RB
cord-328460-thx9zh11	238	25	to	to	IN
cord-328460-thx9zh11	238	26	the	the	DT
cord-328460-thx9zh11	238	27	addition	addition	NN
cord-328460-thx9zh11	238	28	of	of	IN
cord-328460-thx9zh11	238	29	enzymes	enzyme	NNS
cord-328460-thx9zh11	238	30	.	.	.
cord-328460-thx9zh11	239	1	In	in	IN
cord-328460-thx9zh11	239	2	the	the	DT
cord-328460-thx9zh11	239	3	initiation	initiation	NN
cord-328460-thx9zh11	239	4	phase	phase	NN
cord-328460-thx9zh11	239	5	,	,	,
cord-328460-thx9zh11	239	6	a	a	DT
cord-328460-thx9zh11	239	7	specific	specific	JJ
cord-328460-thx9zh11	239	8	forward	forward	JJ
cord-328460-thx9zh11	239	9	primer	primer	NN
cord-328460-thx9zh11	239	10	(	(	-LRB-
cord-328460-thx9zh11	239	11	P1	P1	NNP
cord-328460-thx9zh11	239	12	)	)	-RRB-
cord-328460-thx9zh11	239	13	,	,	,
cord-328460-thx9zh11	239	14	that	that	WDT
cord-328460-thx9zh11	239	15	possesses	possess	VBZ
cord-328460-thx9zh11	239	16	a	a	DT
cord-328460-thx9zh11	239	17	5	5	CD
cord-328460-thx9zh11	239	18	'	'	''
cord-328460-thx9zh11	239	19	sequence	sequence	NN
cord-328460-thx9zh11	239	20	corresponding	correspond	VBG
cord-328460-thx9zh11	239	21	to	to	IN
cord-328460-thx9zh11	239	22	the	the	DT
cord-328460-thx9zh11	239	23	promoter	promoter	NN
cord-328460-thx9zh11	239	24	of	of	IN
cord-328460-thx9zh11	239	25	the	the	DT
cord-328460-thx9zh11	239	26	T7	T7	NNP
cord-328460-thx9zh11	239	27	DdRp	DdRp	NNP
cord-328460-thx9zh11	239	28	,	,	,
cord-328460-thx9zh11	239	29	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	239	30	to	to	IN
cord-328460-thx9zh11	239	31	any	any	DT
cord-328460-thx9zh11	239	32	target	target	NN
cord-328460-thx9zh11	239	33	RNA	RNA	NNP
cord-328460-thx9zh11	239	34	present	present	NN
cord-328460-thx9zh11	239	35	in	in	IN
cord-328460-thx9zh11	239	36	the	the	DT
cord-328460-thx9zh11	239	37	sample	sample	NN
cord-328460-thx9zh11	239	38	and	and	CC
cord-328460-thx9zh11	239	39	is	be	VBZ
cord-328460-thx9zh11	239	40	extended	extend	VBN
cord-328460-thx9zh11	239	41	by	by	IN
cord-328460-thx9zh11	239	42	the	the	DT
cord-328460-thx9zh11	239	43	reverse	reverse	JJ
cord-328460-thx9zh11	239	44	transcriptase	transcriptase	NN
cord-328460-thx9zh11	239	45	.	.	.
cord-328460-thx9zh11	240	1	Subsequently	subsequently	RB
cord-328460-thx9zh11	240	2	,	,	,
cord-328460-thx9zh11	240	3	the	the	DT
cord-328460-thx9zh11	240	4	RNA	RNA	NNP
cord-328460-thx9zh11	240	5	portion	portion	NN
cord-328460-thx9zh11	240	6	of	of	IN
cord-328460-thx9zh11	240	7	the	the	DT
cord-328460-thx9zh11	240	8	resulting	result	VBG
cord-328460-thx9zh11	240	9	RNA	RNA	NNP
cord-328460-thx9zh11	240	10	:	:	:
cord-328460-thx9zh11	240	11	DNA	DNA	NNP
cord-328460-thx9zh11	240	12	heteroduplex	heteroduplex	NNP
cord-328460-thx9zh11	240	13	is	be	VBZ
cord-328460-thx9zh11	240	14	degraded	degrade	VBN
cord-328460-thx9zh11	240	15	by	by	IN
cord-328460-thx9zh11	240	16	RNase	RNase	NNP
cord-328460-thx9zh11	240	17	H	H	NNP
cord-328460-thx9zh11	240	18	,	,	,
cord-328460-thx9zh11	240	19	while	while	IN
cord-328460-thx9zh11	240	20	a	a	DT
cord-328460-thx9zh11	240	21	specific	specific	JJ
cord-328460-thx9zh11	240	22	reverse	reverse	NN
cord-328460-thx9zh11	240	23	primer	primer	NN
cord-328460-thx9zh11	240	24	(	(	-LRB-
cord-328460-thx9zh11	240	25	P2	p2	NN
cord-328460-thx9zh11	240	26	)	)	-RRB-
cord-328460-thx9zh11	240	27	hybridizes	hybridize	VBZ
cord-328460-thx9zh11	240	28	to	to	IN
cord-328460-thx9zh11	240	29	the	the	DT
cord-328460-thx9zh11	240	30	complementary	complementary	JJ
cord-328460-thx9zh11	240	31	sequence	sequence	NN
cord-328460-thx9zh11	240	32	and	and	CC
cord-328460-thx9zh11	240	33	is	be	VBZ
cord-328460-thx9zh11	240	34	extended	extend	VBN
cord-328460-thx9zh11	240	35	by	by	IN
cord-328460-thx9zh11	240	36	the	the	DT
cord-328460-thx9zh11	240	37	reverse	reverse	JJ
cord-328460-thx9zh11	240	38	transcriptase	transcriptase	NN
cord-328460-thx9zh11	240	39	,	,	,
cord-328460-thx9zh11	240	40	leading	lead	VBG
cord-328460-thx9zh11	240	41	to	to	IN
cord-328460-thx9zh11	240	42	the	the	DT
cord-328460-thx9zh11	240	43	formation	formation	NN
cord-328460-thx9zh11	240	44	of	of	IN
cord-328460-thx9zh11	240	45	a	a	DT
cord-328460-thx9zh11	240	46	dsDNA	dsdna	NN
cord-328460-thx9zh11	240	47	with	with	IN
cord-328460-thx9zh11	240	48	the	the	DT
cord-328460-thx9zh11	240	49	target	target	NN
cord-328460-thx9zh11	240	50	sequence	sequence	NN
cord-328460-thx9zh11	240	51	and	and	CC
cord-328460-thx9zh11	240	52	a	a	DT
cord-328460-thx9zh11	240	53	T7	t7	NN
cord-328460-thx9zh11	240	54	promoter	promoter	NN
cord-328460-thx9zh11	240	55	.	.	.
cord-328460-thx9zh11	241	1	Then	then	RB
cord-328460-thx9zh11	241	2	,	,	,
cord-328460-thx9zh11	241	3	the	the	DT
cord-328460-thx9zh11	241	4	T7	T7	NNP
cord-328460-thx9zh11	241	5	DdRp	DdRp	NNP
cord-328460-thx9zh11	241	6	produces	produce	VBZ
cord-328460-thx9zh11	241	7	many	many	JJ
cord-328460-thx9zh11	241	8	RNA	RNA	NNP
cord-328460-thx9zh11	241	9	molecules	molecule	NNS
cord-328460-thx9zh11	241	10	that	that	WDT
cord-328460-thx9zh11	241	11	are	be	VBP
cord-328460-thx9zh11	241	12	complementary	complementary	JJ
cord-328460-thx9zh11	241	13	to	to	IN
cord-328460-thx9zh11	241	14	the	the	DT
cord-328460-thx9zh11	241	15	original	original	JJ
cord-328460-thx9zh11	241	16	target	target	NN
cord-328460-thx9zh11	241	17	RNA	RNA	NNP
cord-328460-thx9zh11	241	18	.	.	.
cord-328460-thx9zh11	242	1	In	in	IN
cord-328460-thx9zh11	242	2	the	the	DT
cord-328460-thx9zh11	242	3	amplification	amplification	NN
cord-328460-thx9zh11	242	4	phase	phase	NN
cord-328460-thx9zh11	242	5	,	,	,
cord-328460-thx9zh11	242	6	each	each	DT
cord-328460-thx9zh11	242	7	newly	newly	RB
cord-328460-thx9zh11	242	8	synthesized	synthesize	VBN
cord-328460-thx9zh11	242	9	RNA	RNA	NNP
cord-328460-thx9zh11	242	10	can	can	MD
cord-328460-thx9zh11	242	11	be	be	VB
cord-328460-thx9zh11	242	12	copied	copy	VBN
cord-328460-thx9zh11	242	13	,	,	,
cord-328460-thx9zh11	242	14	resulting	result	VBG
cord-328460-thx9zh11	242	15	in	in	IN
cord-328460-thx9zh11	242	16	an	an	DT
cord-328460-thx9zh11	242	17	exponential	exponential	JJ
cord-328460-thx9zh11	242	18	amplification	amplification	NN
cord-328460-thx9zh11	242	19	of	of	IN
cord-328460-thx9zh11	242	20	RNA	RNA	NNP
cord-328460-thx9zh11	242	21	complimentary	complimentary	JJ
cord-328460-thx9zh11	242	22	to	to	IN
cord-328460-thx9zh11	242	23	the	the	DT
cord-328460-thx9zh11	242	24	target	target	NN
cord-328460-thx9zh11	242	25	.	.	.
cord-328460-thx9zh11	243	1	Single	single	JJ
cord-328460-thx9zh11	243	2	-	-	HYPH
cord-328460-thx9zh11	243	3	stranded	strand	VBN
cord-328460-thx9zh11	243	4	RNA	RNA	NNP
cord-328460-thx9zh11	243	5	products	product	NNS
cord-328460-thx9zh11	243	6	are	be	VBP
cord-328460-thx9zh11	243	7	detected	detect	VBN
cord-328460-thx9zh11	243	8	by	by	IN
cord-328460-thx9zh11	243	9	using	use	VBG
cord-328460-thx9zh11	243	10	hybridization	hybridization	NN
cord-328460-thx9zh11	243	11	-	-	HYPH
cord-328460-thx9zh11	243	12	based	base	VBN
cord-328460-thx9zh11	243	13	detection	detection	NN
cord-328460-thx9zh11	243	14	protocols	protocol	NNS
cord-328460-thx9zh11	243	15	with	with	IN
cord-328460-thx9zh11	243	16	sequence	sequence	NN
cord-328460-thx9zh11	243	17	-	-	HYPH
cord-328460-thx9zh11	243	18	specific	specific	JJ
cord-328460-thx9zh11	243	19	probes	probe	NNS
cord-328460-thx9zh11	243	20	.	.	.
cord-328460-thx9zh11	244	1	These	these	DT
cord-328460-thx9zh11	244	2	methods	method	NNS
cord-328460-thx9zh11	244	3	include	include	VBP
cord-328460-thx9zh11	244	4	the	the	DT
cord-328460-thx9zh11	244	5	electrochemiluminescence	electrochemiluminescence	NN
cord-328460-thx9zh11	244	6	[	[	-LRB-
cord-328460-thx9zh11	244	7	110	110	CD
cord-328460-thx9zh11	244	8	,	,	,
cord-328460-thx9zh11	244	9	111	111	CD
cord-328460-thx9zh11	244	10	]	]	-RRB-
cord-328460-thx9zh11	244	11	,	,	,
cord-328460-thx9zh11	244	12	the	the	DT
cord-328460-thx9zh11	244	13	lateral	lateral	JJ
cord-328460-thx9zh11	244	14	flow	flow	NN
cord-328460-thx9zh11	244	15	[	[	-LRB-
cord-328460-thx9zh11	244	16	112	112	CD
cord-328460-thx9zh11	244	17	]	]	-RRB-
cord-328460-thx9zh11	244	18	and	and	CC
cord-328460-thx9zh11	244	19	the	the	DT
cord-328460-thx9zh11	244	20	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	244	21	detection	detection	NN
cord-328460-thx9zh11	244	22	[	[	-LRB-
cord-328460-thx9zh11	244	23	113	113	CD
cord-328460-thx9zh11	244	24	]	]	-RRB-
cord-328460-thx9zh11	244	25	.	.	.
cord-328460-thx9zh11	245	1	An	an	DT
cord-328460-thx9zh11	245	2	attractive	attractive	JJ
cord-328460-thx9zh11	245	3	method	method	NN
cord-328460-thx9zh11	245	4	for	for	IN
cord-328460-thx9zh11	245	5	the	the	DT
cord-328460-thx9zh11	245	6	detection	detection	NN
cord-328460-thx9zh11	245	7	of	of	IN
cord-328460-thx9zh11	245	8	NASBA	NASBA	NNP
cord-328460-thx9zh11	245	9	products	product	NNS
cord-328460-thx9zh11	245	10	exploits	exploit	VBZ
cord-328460-thx9zh11	245	11	molecular	molecular	JJ
cord-328460-thx9zh11	245	12	beacons	beacon	NNS
cord-328460-thx9zh11	245	13	(	(	-LRB-
cord-328460-thx9zh11	245	14	MBs	MBs	NNP
cord-328460-thx9zh11	245	15	)	)	-RRB-
cord-328460-thx9zh11	245	16	[	[	-LRB-
cord-328460-thx9zh11	245	17	109	109	CD
cord-328460-thx9zh11	245	18	]	]	-RRB-
cord-328460-thx9zh11	245	19	which	which	WDT
cord-328460-thx9zh11	245	20	are	be	VBP
cord-328460-thx9zh11	245	21	single	single	RB
cord-328460-thx9zh11	245	22	-	-	HYPH
cord-328460-thx9zh11	245	23	stranded	strand	VBN
cord-328460-thx9zh11	245	24	nucleic	nucleic	JJ
cord-328460-thx9zh11	245	25	acid	acid	NN
cord-328460-thx9zh11	245	26	molecules	molecule	NNS
cord-328460-thx9zh11	245	27	that	that	WDT
cord-328460-thx9zh11	245	28	possess	possess	VBP
cord-328460-thx9zh11	245	29	an	an	DT
cord-328460-thx9zh11	245	30	hairpin	hairpin	JJ
cord-328460-thx9zh11	245	31	structure	structure	NN
cord-328460-thx9zh11	245	32	[	[	-LRB-
cord-328460-thx9zh11	245	33	114	114	CD
cord-328460-thx9zh11	245	34	]	]	-RRB-
cord-328460-thx9zh11	245	35	.	.	.
cord-328460-thx9zh11	246	1	A	a	DT
cord-328460-thx9zh11	246	2	fluorophore	fluorophore	NN
cord-328460-thx9zh11	246	3	and	and	CC
cord-328460-thx9zh11	246	4	a	a	DT
cord-328460-thx9zh11	246	5	quencher	quencher	NN
cord-328460-thx9zh11	246	6	moieties	moiety	NNS
cord-328460-thx9zh11	246	7	are	be	VBP
cord-328460-thx9zh11	246	8	kept	keep	VBN
cord-328460-thx9zh11	246	9	in	in	IN
cord-328460-thx9zh11	246	10	close	close	JJ
cord-328460-thx9zh11	246	11	proximity	proximity	NN
cord-328460-thx9zh11	246	12	to	to	IN
cord-328460-thx9zh11	246	13	each	each	DT
cord-328460-thx9zh11	246	14	other	other	JJ
cord-328460-thx9zh11	246	15	due	due	IN
cord-328460-thx9zh11	246	16	to	to	IN
cord-328460-thx9zh11	246	17	the	the	DT
cord-328460-thx9zh11	246	18	stem	stem	NN
cord-328460-thx9zh11	246	19	-	-	HYPH
cord-328460-thx9zh11	246	20	and	and	CC
cord-328460-thx9zh11	246	21	-	-	HYPH
cord-328460-thx9zh11	246	22	loop	loop	NN
cord-328460-thx9zh11	246	23	structure	structure	NN
cord-328460-thx9zh11	246	24	,	,	,
cord-328460-thx9zh11	246	25	so	so	IN
cord-328460-thx9zh11	246	26	that	that	IN
cord-328460-thx9zh11	246	27	the	the	DT
cord-328460-thx9zh11	246	28	fluorescence	fluorescence	NN
cord-328460-thx9zh11	246	29	of	of	IN
cord-328460-thx9zh11	246	30	the	the	DT
cord-328460-thx9zh11	246	31	reporter	reporter	NN
cord-328460-thx9zh11	246	32	is	be	VBZ
cord-328460-thx9zh11	246	33	quenched	quench	VBN
cord-328460-thx9zh11	246	34	by	by	IN
cord-328460-thx9zh11	246	35	fluorescence	fluorescence	NN
cord-328460-thx9zh11	246	36	resonance	resonance	NN
cord-328460-thx9zh11	246	37	energy	energy	NN
cord-328460-thx9zh11	246	38	transfer	transfer	NN
cord-328460-thx9zh11	246	39	(	(	-LRB-
cord-328460-thx9zh11	246	40	FRET	fret	NN
cord-328460-thx9zh11	246	41	)	)	-RRB-
cord-328460-thx9zh11	246	42	to	to	IN
cord-328460-thx9zh11	246	43	the	the	DT
cord-328460-thx9zh11	246	44	quencher	quencher	NN
cord-328460-thx9zh11	246	45	[	[	-LRB-
cord-328460-thx9zh11	246	46	115	115	CD
cord-328460-thx9zh11	246	47	]	]	-RRB-
cord-328460-thx9zh11	246	48	.	.	.
cord-328460-thx9zh11	247	1	Upon	upon	IN
cord-328460-thx9zh11	247	2	probe	probe	NN
cord-328460-thx9zh11	247	3	-	-	HYPH
cord-328460-thx9zh11	247	4	target	target	NN
cord-328460-thx9zh11	247	5	interaction	interaction	NN
cord-328460-thx9zh11	247	6	,	,	,
cord-328460-thx9zh11	247	7	a	a	DT
cord-328460-thx9zh11	247	8	probe	probe	NN
cord-328460-thx9zh11	247	9	conformational	conformational	JJ
cord-328460-thx9zh11	247	10	change	change	NN
cord-328460-thx9zh11	247	11	occurs	occur	VBZ
cord-328460-thx9zh11	247	12	and	and	CC
cord-328460-thx9zh11	247	13	the	the	DT
cord-328460-thx9zh11	247	14	reporter	reporter	NN
cord-328460-thx9zh11	247	15	is	be	VBZ
cord-328460-thx9zh11	247	16	no	no	RB
cord-328460-thx9zh11	247	17	longer	long	RBR
cord-328460-thx9zh11	247	18	in	in	IN
cord-328460-thx9zh11	247	19	close	close	JJ
cord-328460-thx9zh11	247	20	proximity	proximity	NN
cord-328460-thx9zh11	247	21	to	to	IN
cord-328460-thx9zh11	247	22	the	the	DT
cord-328460-thx9zh11	247	23	quencher	quencher	NN
cord-328460-thx9zh11	247	24	,	,	,
cord-328460-thx9zh11	247	25	thus	thus	RB
cord-328460-thx9zh11	247	26	emitting	emit	VBG
cord-328460-thx9zh11	247	27	a	a	DT
cord-328460-thx9zh11	247	28	fluorescent	fluorescent	JJ
cord-328460-thx9zh11	247	29	signal	signal	NN
cord-328460-thx9zh11	247	30	.	.	.
cord-328460-thx9zh11	248	1	Molecular	molecular	JJ
cord-328460-thx9zh11	248	2	beacon	beacon	NN
cord-328460-thx9zh11	248	3	NASBA	NASBA	NNP
cord-328460-thx9zh11	248	4	fluorescence	fluorescence	NN
cord-328460-thx9zh11	248	5	signal	signal	NN
cord-328460-thx9zh11	248	6	can	can	MD
cord-328460-thx9zh11	248	7	be	be	VB
cord-328460-thx9zh11	248	8	detected	detect	VBN
cord-328460-thx9zh11	248	9	in	in	IN
cord-328460-thx9zh11	248	10	real	real	JJ
cord-328460-thx9zh11	248	11	-	-	HYPH
cord-328460-thx9zh11	248	12	time	time	NN
cord-328460-thx9zh11	248	13	and	and	CC
cord-328460-thx9zh11	248	14	is	be	VBZ
cord-328460-thx9zh11	248	15	proportional	proportional	JJ
cord-328460-thx9zh11	248	16	to	to	IN
cord-328460-thx9zh11	248	17	the	the	DT
cord-328460-thx9zh11	248	18	amount	amount	NN
cord-328460-thx9zh11	248	19	of	of	IN
cord-328460-thx9zh11	248	20	target	target	NN
cord-328460-thx9zh11	248	21	RNA	rna	NN
cord-328460-thx9zh11	248	22	sequence	sequence	NN
cord-328460-thx9zh11	248	23	[	[	-LRB-
cord-328460-thx9zh11	248	24	116	116	CD
cord-328460-thx9zh11	248	25	,	,	,
cord-328460-thx9zh11	248	26	117	117	CD
cord-328460-thx9zh11	248	27	]	]	-RRB-
cord-328460-thx9zh11	248	28	.	.	.
cord-328460-thx9zh11	249	1	Real	real	JJ
cord-328460-thx9zh11	249	2	-	-	HYPH
cord-328460-thx9zh11	249	3	time	time	NN
cord-328460-thx9zh11	249	4	NASBA	NASBA	NNP
cord-328460-thx9zh11	249	5	technology	technology	NN
cord-328460-thx9zh11	249	6	has	have	VBZ
cord-328460-thx9zh11	249	7	been	be	VBN
cord-328460-thx9zh11	249	8	integrated	integrate	VBN
cord-328460-thx9zh11	249	9	in	in	IN
cord-328460-thx9zh11	249	10	a	a	DT
cord-328460-thx9zh11	249	11	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	249	12	system	system	NN
cord-328460-thx9zh11	249	13	by	by	IN
cord-328460-thx9zh11	249	14	Gulliksen	Gulliksen	NNP
cord-328460-thx9zh11	249	15	et	et	NNP
cord-328460-thx9zh11	249	16	al	al	NNP
cord-328460-thx9zh11	249	17	.	.	.
cord-328460-thx9zh11	250	1	[	[	-LRB-
cord-328460-thx9zh11	250	2	118	118	CD
cord-328460-thx9zh11	250	3	]	]	-RRB-
cord-328460-thx9zh11	250	4	.	.	.
cord-328460-thx9zh11	251	1	In	in	IN
cord-328460-thx9zh11	251	2	this	this	DT
cord-328460-thx9zh11	251	3	case	case	NN
cord-328460-thx9zh11	251	4	,	,	,
cord-328460-thx9zh11	251	5	the	the	DT
cord-328460-thx9zh11	251	6	amplification	amplification	NN
cord-328460-thx9zh11	251	7	of	of	IN
cord-328460-thx9zh11	251	8	1.0	1.0	CD
cord-328460-thx9zh11	251	9	and	and	CC
cord-328460-thx9zh11	251	10	0.1	0.1	CD
cord-328460-thx9zh11	251	11	ìM	ìm	CD
cord-328460-thx9zh11	251	12	oligonucleotides	oligonucleotide	NNS
cord-328460-thx9zh11	251	13	(	(	-LRB-
cord-328460-thx9zh11	251	14	human	human	JJ
cord-328460-thx9zh11	251	15	papillomavirus	papillomavirus	NN
cord-328460-thx9zh11	251	16	(	(	-LRB-
cord-328460-thx9zh11	251	17	HPV	HPV	NNP
cord-328460-thx9zh11	251	18	)	)	-RRB-
cord-328460-thx9zh11	251	19	16	16	CD
cord-328460-thx9zh11	251	20	and	and	CC
cord-328460-thx9zh11	251	21	artificial	artificial	JJ
cord-328460-thx9zh11	251	22	118-bp	118-bp	CD
cord-328460-thx9zh11	251	23	single	single	RB
cord-328460-thx9zh11	251	24	-	-	HYPH
cord-328460-thx9zh11	251	25	stranded	strand	VBN
cord-328460-thx9zh11	251	26	DNA	dna	NN
cord-328460-thx9zh11	251	27	)	)	-RRB-
cord-328460-thx9zh11	251	28	was	be	VBD
cord-328460-thx9zh11	251	29	performed	perform	VBN
cord-328460-thx9zh11	251	30	in	in	IN
cord-328460-thx9zh11	251	31	a	a	DT
cord-328460-thx9zh11	251	32	silicon	silicon	NN
cord-328460-thx9zh11	251	33	-	-	HYPH
cord-328460-thx9zh11	251	34	glass	glass	NN
cord-328460-thx9zh11	251	35	microchip	microchip	NN
cord-328460-thx9zh11	251	36	device	device	NN
cord-328460-thx9zh11	251	37	comprising	comprise	VBG
cord-328460-thx9zh11	251	38	heated	heat	VBD
cord-328460-thx9zh11	251	39	10	10	CD
cord-328460-thx9zh11	251	40	nL	nL	NNP
cord-328460-thx9zh11	251	41	and	and	CC
cord-328460-thx9zh11	251	42	50	50	CD
cord-328460-thx9zh11	251	43	nL	nL	NNP
cord-328460-thx9zh11	251	44	reaction	reaction	NN
cord-328460-thx9zh11	251	45	chambers	chamber	NNS
cord-328460-thx9zh11	251	46	.	.	.
cord-328460-thx9zh11	252	1	A	a	DT
cord-328460-thx9zh11	252	2	surface	surface	NN
cord-328460-thx9zh11	252	3	treatment	treatment	NN
cord-328460-thx9zh11	252	4	was	be	VBD
cord-328460-thx9zh11	252	5	required	require	VBN
cord-328460-thx9zh11	252	6	in	in	IN
cord-328460-thx9zh11	252	7	order	order	NN
cord-328460-thx9zh11	252	8	to	to	TO
cord-328460-thx9zh11	252	9	prevent	prevent	VB
cord-328460-thx9zh11	252	10	the	the	DT
cord-328460-thx9zh11	252	11	adsorption	adsorption	NN
cord-328460-thx9zh11	252	12	of	of	IN
cord-328460-thx9zh11	252	13	reaction	reaction	NN
cord-328460-thx9zh11	252	14	mixture	mixture	NN
cord-328460-thx9zh11	252	15	components	component	NNS
cord-328460-thx9zh11	252	16	on	on	IN
cord-328460-thx9zh11	252	17	the	the	DT
cord-328460-thx9zh11	252	18	surface	surface	NN
cord-328460-thx9zh11	252	19	of	of	IN
cord-328460-thx9zh11	252	20	the	the	DT
cord-328460-thx9zh11	252	21	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	252	22	device	device	NN
cord-328460-thx9zh11	252	23	.	.	.
cord-328460-thx9zh11	253	1	An	an	DT
cord-328460-thx9zh11	253	2	improvement	improvement	NN
cord-328460-thx9zh11	253	3	in	in	IN
cord-328460-thx9zh11	253	4	the	the	DT
cord-328460-thx9zh11	253	5	real	real	JJ
cord-328460-thx9zh11	253	6	-	-	HYPH
cord-328460-thx9zh11	253	7	time	time	NN
cord-328460-thx9zh11	253	8	NASBA	NASBA	NNP
cord-328460-thx9zh11	253	9	integration	integration	NN
cord-328460-thx9zh11	253	10	in	in	IN
cord-328460-thx9zh11	253	11	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	253	12	environment	environment	NN
cord-328460-thx9zh11	253	13	was	be	VBD
cord-328460-thx9zh11	253	14	introduced	introduce	VBN
cord-328460-thx9zh11	253	15	by	by	IN
cord-328460-thx9zh11	253	16	detecting	detect	VBG
cord-328460-thx9zh11	253	17	artificial	artificial	JJ
cord-328460-thx9zh11	253	18	human	human	JJ
cord-328460-thx9zh11	253	19	papilloma	papilloma	NN
cord-328460-thx9zh11	253	20	virus	virus	NN
cord-328460-thx9zh11	253	21	(	(	-LRB-
cord-328460-thx9zh11	253	22	HPV	hpv	NN
cord-328460-thx9zh11	253	23	)	)	-RRB-
cord-328460-thx9zh11	254	1	16	16	CD
cord-328460-thx9zh11	254	2	sequences	sequence	NNS
cord-328460-thx9zh11	254	3	and	and	CC
cord-328460-thx9zh11	254	4	SiHa	SiHa	NNP
cord-328460-thx9zh11	254	5	cell	cell	NN
cord-328460-thx9zh11	254	6	line	line	NN
cord-328460-thx9zh11	254	7	samples	sample	NNS
cord-328460-thx9zh11	254	8	in	in	IN
cord-328460-thx9zh11	254	9	cyclic	cyclic	JJ
cord-328460-thx9zh11	254	10	olefin	olefin	NN
cord-328460-thx9zh11	254	11	copolymer	copolymer	NN
cord-328460-thx9zh11	254	12	(	(	-LRB-
cord-328460-thx9zh11	254	13	COC	COC	NNP
cord-328460-thx9zh11	254	14	)	)	-RRB-
cord-328460-thx9zh11	254	15	microchips	microchip	NNS
cord-328460-thx9zh11	254	16	[	[	-LRB-
cord-328460-thx9zh11	254	17	119	119	CD
cord-328460-thx9zh11	254	18	]	]	-RRB-
cord-328460-thx9zh11	254	19	.	.	.
cord-328460-thx9zh11	255	1	In	in	IN
cord-328460-thx9zh11	255	2	this	this	DT
cord-328460-thx9zh11	255	3	multipurpose	multipurpose	JJ
cord-328460-thx9zh11	255	4	microchip	microchip	NN
cord-328460-thx9zh11	255	5	platform	platform	NN
cord-328460-thx9zh11	255	6	,	,	,
cord-328460-thx9zh11	255	7	the	the	DT
cord-328460-thx9zh11	255	8	sample	sample	NN
cord-328460-thx9zh11	255	9	was	be	VBD
cord-328460-thx9zh11	255	10	automatically	automatically	RB
cord-328460-thx9zh11	255	11	distributed	distribute	VBN
cord-328460-thx9zh11	255	12	into	into	IN
cord-328460-thx9zh11	255	13	10	10	CD
cord-328460-thx9zh11	255	14	parallel	parallel	JJ
cord-328460-thx9zh11	255	15	reaction	reaction	NN
cord-328460-thx9zh11	255	16	channels	channel	NNS
cord-328460-thx9zh11	255	17	for	for	IN
cord-328460-thx9zh11	255	18	simultaneous	simultaneous	JJ
cord-328460-thx9zh11	255	19	detection	detection	NN
cord-328460-thx9zh11	255	20	in	in	IN
cord-328460-thx9zh11	255	21	80	80	CD
cord-328460-thx9zh11	255	22	nL	nL	NNP
cord-328460-thx9zh11	255	23	volumes	volume	NNS
cord-328460-thx9zh11	255	24	,	,	,
cord-328460-thx9zh11	255	25	making	make	VBG
cord-328460-thx9zh11	255	26	it	-PRON-	PRP
cord-328460-thx9zh11	255	27	possible	possible	JJ
cord-328460-thx9zh11	255	28	to	to	TO
cord-328460-thx9zh11	255	29	specifically	specifically	RB
cord-328460-thx9zh11	255	30	amplify	amplify	VB
cord-328460-thx9zh11	255	31	and	and	CC
cord-328460-thx9zh11	255	32	detect	detect	VB
cord-328460-thx9zh11	255	33	several	several	JJ
cord-328460-thx9zh11	255	34	different	different	JJ
cord-328460-thx9zh11	255	35	targets	target	NNS
cord-328460-thx9zh11	255	36	with	with	IN
cord-328460-thx9zh11	255	37	high	high	JJ
cord-328460-thx9zh11	255	38	sensitivity	sensitivity	NN
cord-328460-thx9zh11	255	39	by	by	IN
cord-328460-thx9zh11	255	40	only	only	RB
cord-328460-thx9zh11	255	41	using	use	VBG
cord-328460-thx9zh11	255	42	one	one	CD
cord-328460-thx9zh11	255	43	single	single	JJ
cord-328460-thx9zh11	255	44	sample	sample	NN
cord-328460-thx9zh11	255	45	.	.	.
cord-328460-thx9zh11	256	1	More	more	RBR
cord-328460-thx9zh11	256	2	recently	recently	RB
cord-328460-thx9zh11	256	3	Gulliksen	Gulliksen	NNP
cord-328460-thx9zh11	256	4	et	et	NNP
cord-328460-thx9zh11	256	5	al	al	NNP
cord-328460-thx9zh11	256	6	.	.	.
cord-328460-thx9zh11	257	1	presented	present	VBD
cord-328460-thx9zh11	257	2	the	the	DT
cord-328460-thx9zh11	257	3	development	development	NN
cord-328460-thx9zh11	257	4	of	of	IN
cord-328460-thx9zh11	257	5	a	a	DT
cord-328460-thx9zh11	257	6	"	"	``
cord-328460-thx9zh11	257	7	proof	proof	NN
cord-328460-thx9zh11	257	8	of	of	IN
cord-328460-thx9zh11	257	9	principle	principle	NN
cord-328460-thx9zh11	257	10	"	"	``
cord-328460-thx9zh11	257	11	self	self	NN
cord-328460-thx9zh11	257	12	-	-	HYPH
cord-328460-thx9zh11	257	13	contained	contain	VBN
cord-328460-thx9zh11	257	14	and	and	CC
cord-328460-thx9zh11	257	15	hands	hand	NNS
cord-328460-thx9zh11	257	16	-	-	HYPH
cord-328460-thx9zh11	257	17	free	free	JJ
cord-328460-thx9zh11	257	18	diagnostic	diagnostic	JJ
cord-328460-thx9zh11	257	19	platform	platform	NN
cord-328460-thx9zh11	257	20	for	for	IN
cord-328460-thx9zh11	257	21	NASBA	NASBA	NNP
cord-328460-thx9zh11	257	22	which	which	WDT
cord-328460-thx9zh11	257	23	performs	perform	VBZ
cord-328460-thx9zh11	257	24	sample	sample	NN
cord-328460-thx9zh11	257	25	preconcentration	preconcentration	NN
cord-328460-thx9zh11	257	26	,	,	,
cord-328460-thx9zh11	257	27	nucleic	nucleic	JJ
cord-328460-thx9zh11	257	28	acid	acid	NN
cord-328460-thx9zh11	257	29	extraction	extraction	NN
cord-328460-thx9zh11	257	30	,	,	,
cord-328460-thx9zh11	257	31	amplification	amplification	NN
cord-328460-thx9zh11	257	32	and	and	CC
cord-328460-thx9zh11	257	33	real	real	JJ
cord-328460-thx9zh11	257	34	-	-	HYPH
cord-328460-thx9zh11	257	35	time	time	NN
cord-328460-thx9zh11	257	36	fluorescent	fluorescent	NN
cord-328460-thx9zh11	257	37	detection	detection	NN
cord-328460-thx9zh11	257	38	with	with	IN
cord-328460-thx9zh11	257	39	minimal	minimal	JJ
cord-328460-thx9zh11	257	40	user	user	NN
cord-328460-thx9zh11	257	41	interfacing	interface	VBG
cord-328460-thx9zh11	257	42	[	[	-LRB-
cord-328460-thx9zh11	257	43	120	120	CD
cord-328460-thx9zh11	257	44	]	]	-RRB-
cord-328460-thx9zh11	257	45	.	.	.
cord-328460-thx9zh11	258	1	The	the	DT
cord-328460-thx9zh11	258	2	automated	automated	JJ
cord-328460-thx9zh11	258	3	platform	platform	NN
cord-328460-thx9zh11	258	4	comprises	comprise	VBZ
cord-328460-thx9zh11	258	5	two	two	CD
cord-328460-thx9zh11	258	6	distinct	distinct	JJ
cord-328460-thx9zh11	258	7	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	258	8	chips	chip	NNS
cord-328460-thx9zh11	258	9	,	,	,
cord-328460-thx9zh11	258	10	one	one	CD
cord-328460-thx9zh11	258	11	for	for	IN
cord-328460-thx9zh11	258	12	sample	sample	NN
cord-328460-thx9zh11	258	13	preparation	preparation	NN
cord-328460-thx9zh11	258	14	and	and	CC
cord-328460-thx9zh11	258	15	the	the	DT
cord-328460-thx9zh11	258	16	other	other	JJ
cord-328460-thx9zh11	258	17	for	for	IN
cord-328460-thx9zh11	258	18	nucleic	nucleic	JJ
cord-328460-thx9zh11	258	19	acid	acid	NN
cord-328460-thx9zh11	258	20	amplification	amplification	NN
cord-328460-thx9zh11	258	21	,	,	,
cord-328460-thx9zh11	258	22	and	and	CC
cord-328460-thx9zh11	258	23	was	be	VBD
cord-328460-thx9zh11	258	24	applied	apply	VBN
cord-328460-thx9zh11	258	25	to	to	IN
cord-328460-thx9zh11	258	26	the	the	DT
cord-328460-thx9zh11	258	27	detection	detection	NN
cord-328460-thx9zh11	258	28	of	of	IN
cord-328460-thx9zh11	258	29	HPV	HPV	NNP
cord-328460-thx9zh11	258	30	mRNA	mrna	NN
cord-328460-thx9zh11	258	31	in	in	IN
cord-328460-thx9zh11	258	32	clinical	clinical	JJ
cord-328460-thx9zh11	258	33	specimens	specimen	NNS
cord-328460-thx9zh11	258	34	.	.	.
cord-328460-thx9zh11	259	1	A	a	DT
cord-328460-thx9zh11	259	2	more	more	RBR
cord-328460-thx9zh11	259	3	complex	complex	JJ
cord-328460-thx9zh11	259	4	device	device	NN
cord-328460-thx9zh11	259	5	was	be	VBD
cord-328460-thx9zh11	259	6	obtained	obtain	VBN
cord-328460-thx9zh11	259	7	after	after	IN
cord-328460-thx9zh11	259	8	the	the	DT
cord-328460-thx9zh11	259	9	monolithic	monolithic	JJ
cord-328460-thx9zh11	259	10	integration	integration	NN
cord-328460-thx9zh11	259	11	of	of	IN
cord-328460-thx9zh11	259	12	a	a	DT
cord-328460-thx9zh11	259	13	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	259	14	sample	sample	NN
cord-328460-thx9zh11	259	15	-	-	HYPH
cord-328460-thx9zh11	259	16	to	to	IN
cord-328460-thx9zh11	259	17	-	-	HYPH
cord-328460-thx9zh11	259	18	answer	answer	NN
cord-328460-thx9zh11	259	19	transfer	transfer	NN
cord-328460-thx9zh11	259	20	-	-	HYPH
cord-328460-thx9zh11	259	21	mRNA	mrna	NN
cord-328460-thx9zh11	259	22	(	(	-LRB-
cord-328460-thx9zh11	259	23	tmRNA	tmrna	NN
cord-328460-thx9zh11	259	24	)	)	-RRB-
cord-328460-thx9zh11	259	25	analysis	analysis	NN
cord-328460-thx9zh11	259	26	system	system	NN
cord-328460-thx9zh11	259	27	that	that	WDT
cord-328460-thx9zh11	259	28	included	include	VBD
cord-328460-thx9zh11	259	29	RNA	RNA	NNP
cord-328460-thx9zh11	259	30	capture	capture	NN
cord-328460-thx9zh11	259	31	and	and	CC
cord-328460-thx9zh11	259	32	purification	purification	NN
cord-328460-thx9zh11	259	33	,	,	,
cord-328460-thx9zh11	259	34	NASBA	NASBA	NNP
cord-328460-thx9zh11	259	35	amplification	amplification	NN
cord-328460-thx9zh11	259	36	,	,	,
cord-328460-thx9zh11	259	37	and	and	CC
cord-328460-thx9zh11	259	38	real	real	JJ
cord-328460-thx9zh11	259	39	-	-	HYPH
cord-328460-thx9zh11	259	40	time	time	NN
cord-328460-thx9zh11	259	41	detection	detection	NN
cord-328460-thx9zh11	259	42	[	[	-LRB-
cord-328460-thx9zh11	259	43	121	121	CD
cord-328460-thx9zh11	259	44	]	]	-RRB-
cord-328460-thx9zh11	259	45	.	.	.
cord-328460-thx9zh11	260	1	The	the	DT
cord-328460-thx9zh11	260	2	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	260	3	NASBA	NASBA	NNP
cord-328460-thx9zh11	260	4	system	system	NN
cord-328460-thx9zh11	260	5	was	be	VBD
cord-328460-thx9zh11	260	6	based	base	VBN
cord-328460-thx9zh11	260	7	on	on	IN
cord-328460-thx9zh11	260	8	microchannels	microchannel	NNS
cord-328460-thx9zh11	260	9	and	and	CC
cord-328460-thx9zh11	260	10	microchambers	microchamber	NNS
cord-328460-thx9zh11	260	11	,	,	,
cord-328460-thx9zh11	260	12	with	with	IN
cord-328460-thx9zh11	260	13	distinct	distinct	JJ
cord-328460-thx9zh11	260	14	functional	functional	JJ
cord-328460-thx9zh11	260	15	domains	domain	NNS
cord-328460-thx9zh11	260	16	:	:	:
cord-328460-thx9zh11	260	17	a	a	DT
cord-328460-thx9zh11	260	18	silica	silica	NN
cord-328460-thx9zh11	260	19	bead	bead	NN
cord-328460-thx9zh11	260	20	-	-	HYPH
cord-328460-thx9zh11	260	21	bed	bed	NN
cord-328460-thx9zh11	260	22	RNA	RNA	NNP
cord-328460-thx9zh11	260	23	purification	purification	NN
cord-328460-thx9zh11	260	24	chamber	chamber	NN
cord-328460-thx9zh11	260	25	(	(	-LRB-
cord-328460-thx9zh11	260	26	volume	volume	NN
cord-328460-thx9zh11	260	27	0.25	0.25	CD
cord-328460-thx9zh11	260	28	µL	µL	NNP
cord-328460-thx9zh11	260	29	)	)	-RRB-
cord-328460-thx9zh11	260	30	,	,	,
cord-328460-thx9zh11	260	31	that	that	WDT
cord-328460-thx9zh11	260	32	enabled	enable	VBD
cord-328460-thx9zh11	260	33	the	the	DT
cord-328460-thx9zh11	260	34	capture	capture	NN
cord-328460-thx9zh11	260	35	of	of	IN
cord-328460-thx9zh11	260	36	RNA	RNA	NNP
cord-328460-thx9zh11	260	37	by	by	IN
cord-328460-thx9zh11	260	38	its	-PRON-	PRP$
cord-328460-thx9zh11	260	39	selective	selective	JJ
cord-328460-thx9zh11	260	40	adsorption	adsorption	NN
cord-328460-thx9zh11	260	41	on	on	IN
cord-328460-thx9zh11	260	42	silica	silica	NN
cord-328460-thx9zh11	260	43	,	,	,
cord-328460-thx9zh11	260	44	and	and	CC
cord-328460-thx9zh11	260	45	a	a	DT
cord-328460-thx9zh11	260	46	NASBA	NASBA	NNP
cord-328460-thx9zh11	260	47	chamber	chamber	NN
cord-328460-thx9zh11	260	48	(	(	-LRB-
cord-328460-thx9zh11	260	49	volume	volume	NN
cord-328460-thx9zh11	260	50	2	2	CD
cord-328460-thx9zh11	260	51	µL	µl	CD
cord-328460-thx9zh11	260	52	)	)	-RRB-
cord-328460-thx9zh11	260	53	,	,	,
cord-328460-thx9zh11	260	54	where	where	WRB
cord-328460-thx9zh11	260	55	the	the	DT
cord-328460-thx9zh11	260	56	amplified	amplify	VBN
cord-328460-thx9zh11	260	57	tmRNA	tmRNA	VBG
cord-328460-thx9zh11	260	58	target	target	NN
cord-328460-thx9zh11	260	59	was	be	VBD
cord-328460-thx9zh11	260	60	detected	detect	VBN
cord-328460-thx9zh11	260	61	in	in	IN
cord-328460-thx9zh11	260	62	real	real	JJ
cord-328460-thx9zh11	260	63	-	-	HYPH
cord-328460-thx9zh11	260	64	time	time	NN
cord-328460-thx9zh11	260	65	via	via	IN
cord-328460-thx9zh11	260	66	molecular	molecular	JJ
cord-328460-thx9zh11	260	67	beacons	beacon	NNS
cord-328460-thx9zh11	260	68	fluorescence	fluorescence	NN
cord-328460-thx9zh11	260	69	detection	detection	NN
cord-328460-thx9zh11	260	70	.	.	.
cord-328460-thx9zh11	261	1	The	the	DT
cord-328460-thx9zh11	261	2	integration	integration	NN
cord-328460-thx9zh11	261	3	of	of	IN
cord-328460-thx9zh11	261	4	the	the	DT
cord-328460-thx9zh11	261	5	sample	sample	NN
cord-328460-thx9zh11	261	6	preparation	preparation	NN
cord-328460-thx9zh11	261	7	,	,	,
cord-328460-thx9zh11	261	8	amplification	amplification	NN
cord-328460-thx9zh11	261	9	and	and	CC
cord-328460-thx9zh11	261	10	detection	detection	NN
cord-328460-thx9zh11	261	11	compartments	compartment	NNS
cord-328460-thx9zh11	261	12	in	in	IN
cord-328460-thx9zh11	261	13	a	a	DT
cord-328460-thx9zh11	261	14	single	single	JJ
cord-328460-thx9zh11	261	15	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	261	16	device	device	NN
cord-328460-thx9zh11	261	17	reduces	reduce	VBZ
cord-328460-thx9zh11	261	18	the	the	DT
cord-328460-thx9zh11	261	19	risk	risk	NN
cord-328460-thx9zh11	261	20	of	of	IN
cord-328460-thx9zh11	261	21	sample	sample	NN
cord-328460-thx9zh11	261	22	degradation	degradation	NN
cord-328460-thx9zh11	261	23	,	,	,
cord-328460-thx9zh11	261	24	which	which	WDT
cord-328460-thx9zh11	261	25	can	can	MD
cord-328460-thx9zh11	261	26	compromise	compromise	VB
cord-328460-thx9zh11	261	27	assay	assay	NN
cord-328460-thx9zh11	261	28	accuracy	accuracy	NN
cord-328460-thx9zh11	261	29	,	,	,
cord-328460-thx9zh11	261	30	reliability	reliability	NN
cord-328460-thx9zh11	261	31	and	and	CC
cord-328460-thx9zh11	261	32	reproducibility	reproducibility	NN
cord-328460-thx9zh11	261	33	.	.	.
cord-328460-thx9zh11	262	1	A	a	DT
cord-328460-thx9zh11	262	2	lab	lab	NN
cord-328460-thx9zh11	262	3	-	-	HYPH
cord-328460-thx9zh11	262	4	on	on	IN
cord-328460-thx9zh11	262	5	-	-	HYPH
cord-328460-thx9zh11	262	6	a	a	DT
cord-328460-thx9zh11	262	7	-	-	HYPH
cord-328460-thx9zh11	262	8	chip	chip	NN
cord-328460-thx9zh11	262	9	(	(	-LRB-
cord-328460-thx9zh11	262	10	LOC	LOC	NNP
cord-328460-thx9zh11	262	11	)	)	-RRB-
cord-328460-thx9zh11	262	12	device	device	NN
cord-328460-thx9zh11	262	13	based	base	VBN
cord-328460-thx9zh11	262	14	on	on	IN
cord-328460-thx9zh11	262	15	the	the	DT
cord-328460-thx9zh11	262	16	real	real	JJ
cord-328460-thx9zh11	262	17	-	-	HYPH
cord-328460-thx9zh11	262	18	time	time	NN
cord-328460-thx9zh11	262	19	immuno	immuno	JJ
cord-328460-thx9zh11	262	20	nucleic	nucleic	JJ
cord-328460-thx9zh11	262	21	acid	acid	NN
cord-328460-thx9zh11	262	22	sequence	sequence	NN
cord-328460-thx9zh11	262	23	-	-	HYPH
cord-328460-thx9zh11	262	24	based	base	VBN
cord-328460-thx9zh11	262	25	amplification	amplification	NN
cord-328460-thx9zh11	262	26	(	(	-LRB-
cord-328460-thx9zh11	262	27	immuno	immuno	JJ
cord-328460-thx9zh11	262	28	-	-	HYPH
cord-328460-thx9zh11	262	29	NASBA	nasba	NN
cord-328460-thx9zh11	262	30	)	)	-RRB-
cord-328460-thx9zh11	262	31	for	for	IN
cord-328460-thx9zh11	262	32	quantitative	quantitative	JJ
cord-328460-thx9zh11	262	33	detection	detection	NN
cord-328460-thx9zh11	262	34	of	of	IN
cord-328460-thx9zh11	262	35	waterborne	waterborne	JJ
cord-328460-thx9zh11	262	36	pathogens	pathogen	NNS
cord-328460-thx9zh11	262	37	has	have	VBZ
cord-328460-thx9zh11	262	38	been	be	VBN
cord-328460-thx9zh11	262	39	recently	recently	RB
cord-328460-thx9zh11	262	40	designed	design	VBN
cord-328460-thx9zh11	262	41	by	by	IN
cord-328460-thx9zh11	262	42	Zhao	Zhao	NNP
cord-328460-thx9zh11	262	43	et	et	NNP
cord-328460-thx9zh11	262	44	al	al	NNP
cord-328460-thx9zh11	262	45	.	.	.
cord-328460-thx9zh11	263	1	[	[	-LRB-
cord-328460-thx9zh11	263	2	122	122	CD
cord-328460-thx9zh11	263	3	]	]	-RRB-
cord-328460-thx9zh11	263	4	.	.	.
cord-328460-thx9zh11	264	1	The	the	DT
cord-328460-thx9zh11	264	2	immuno	immuno	JJ
cord-328460-thx9zh11	264	3	-	-	HYPH
cord-328460-thx9zh11	264	4	NASBA	nasba	NN
cord-328460-thx9zh11	264	5	assays	assay	NNS
cord-328460-thx9zh11	264	6	relies	rely	VBZ
cord-328460-thx9zh11	264	7	on	on	IN
cord-328460-thx9zh11	264	8	the	the	DT
cord-328460-thx9zh11	264	9	use	use	NN
cord-328460-thx9zh11	264	10	of	of	IN
cord-328460-thx9zh11	264	11	different	different	JJ
cord-328460-thx9zh11	264	12	antibodies	antibody	NNS
cord-328460-thx9zh11	264	13	to	to	TO
cord-328460-thx9zh11	264	14	recognize	recognize	VB
cord-328460-thx9zh11	264	15	different	different	JJ
cord-328460-thx9zh11	264	16	bio	bio	NN
cord-328460-thx9zh11	264	17	-	-	HYPH
cord-328460-thx9zh11	264	18	targets	target	NNS
cord-328460-thx9zh11	264	19	and	and	CC
cord-328460-thx9zh11	264	20	on	on	IN
cord-328460-thx9zh11	264	21	signal	signal	JJ
cord-328460-thx9zh11	264	22	amplification	amplification	NN
cord-328460-thx9zh11	264	23	by	by	IN
cord-328460-thx9zh11	264	24	NASBA	NASBA	NNP
cord-328460-thx9zh11	264	25	reaction	reaction	NN
cord-328460-thx9zh11	264	26	before	before	IN
cord-328460-thx9zh11	264	27	the	the	DT
cord-328460-thx9zh11	264	28	detection	detection	NN
cord-328460-thx9zh11	264	29	.	.	.
cord-328460-thx9zh11	265	1	This	this	DT
cord-328460-thx9zh11	265	2	approach	approach	NN
cord-328460-thx9zh11	265	3	enables	enable	VBZ
cord-328460-thx9zh11	265	4	the	the	DT
cord-328460-thx9zh11	265	5	quantification	quantification	NN
cord-328460-thx9zh11	265	6	of	of	IN
cord-328460-thx9zh11	265	7	traces	trace	NNS
cord-328460-thx9zh11	265	8	of	of	IN
cord-328460-thx9zh11	265	9	waterborne	waterborne	JJ
cord-328460-thx9zh11	265	10	pathogens	pathogen	NNS
cord-328460-thx9zh11	265	11	at	at	IN
cord-328460-thx9zh11	265	12	femtomolar	femtomolar	JJ
cord-328460-thx9zh11	265	13	levels	level	NNS
cord-328460-thx9zh11	265	14	with	with	IN
cord-328460-thx9zh11	265	15	high	high	JJ
cord-328460-thx9zh11	265	16	specificity	specificity	NN
cord-328460-thx9zh11	265	17	.	.	.
cord-328460-thx9zh11	266	1	One	one	CD
cord-328460-thx9zh11	266	2	of	of	IN
cord-328460-thx9zh11	266	3	the	the	DT
cord-328460-thx9zh11	266	4	main	main	JJ
cord-328460-thx9zh11	266	5	advantages	advantage	NNS
cord-328460-thx9zh11	266	6	of	of	IN
cord-328460-thx9zh11	266	7	the	the	DT
cord-328460-thx9zh11	266	8	developed	develop	VBN
cord-328460-thx9zh11	266	9	system	system	NN
cord-328460-thx9zh11	266	10	is	be	VBZ
cord-328460-thx9zh11	266	11	that	that	IN
cord-328460-thx9zh11	266	12	the	the	DT
cord-328460-thx9zh11	266	13	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	266	14	device	device	NN
cord-328460-thx9zh11	266	15	is	be	VBZ
cord-328460-thx9zh11	266	16	modelled	model	VBN
cord-328460-thx9zh11	266	17	on	on	IN
cord-328460-thx9zh11	266	18	a	a	DT
cord-328460-thx9zh11	266	19	96-well	96-well	CD
cord-328460-thx9zh11	266	20	ELISA	ELISA	NNP
cord-328460-thx9zh11	266	21	microplate	microplate	NN
cord-328460-thx9zh11	266	22	,	,	,
cord-328460-thx9zh11	266	23	so	so	CC
cord-328460-thx9zh11	266	24	it	-PRON-	PRP
cord-328460-thx9zh11	266	25	is	be	VBZ
cord-328460-thx9zh11	266	26	compatible	compatible	JJ
cord-328460-thx9zh11	266	27	with	with	IN
cord-328460-thx9zh11	266	28	the	the	DT
cord-328460-thx9zh11	266	29	conventional	conventional	JJ
cord-328460-thx9zh11	266	30	equipment	equipment	NN
cord-328460-thx9zh11	266	31	in	in	IN
cord-328460-thx9zh11	266	32	a	a	DT
cord-328460-thx9zh11	266	33	biological	biological	JJ
cord-328460-thx9zh11	266	34	laboratory	laboratory	NN
cord-328460-thx9zh11	266	35	.	.	.
cord-328460-thx9zh11	267	1	On	on	IN
cord-328460-thx9zh11	267	2	the	the	DT
cord-328460-thx9zh11	267	3	other	other	JJ
cord-328460-thx9zh11	267	4	hand	hand	NN
cord-328460-thx9zh11	267	5	,	,	,
cord-328460-thx9zh11	267	6	it	-PRON-	PRP
cord-328460-thx9zh11	267	7	is	be	VBZ
cord-328460-thx9zh11	267	8	not	not	RB
cord-328460-thx9zh11	267	9	free	free	JJ
cord-328460-thx9zh11	267	10	from	from	IN
cord-328460-thx9zh11	267	11	drawbacks	drawback	NNS
cord-328460-thx9zh11	267	12	,	,	,
cord-328460-thx9zh11	267	13	since	since	IN
cord-328460-thx9zh11	267	14	it	-PRON-	PRP
cord-328460-thx9zh11	267	15	is	be	VBZ
cord-328460-thx9zh11	267	16	prone	prone	JJ
cord-328460-thx9zh11	267	17	to	to	IN
cord-328460-thx9zh11	267	18	ribonuclease	ribonuclease	NN
cord-328460-thx9zh11	267	19	(	(	-LRB-
cord-328460-thx9zh11	267	20	RNase	rnase	NN
cord-328460-thx9zh11	267	21	)	)	-RRB-
cord-328460-thx9zh11	267	22	contamination	contamination	NN
cord-328460-thx9zh11	267	23	and	and	CC
cord-328460-thx9zh11	267	24	non	non	JJ
cord-328460-thx9zh11	267	25	-	-	JJ
cord-328460-thx9zh11	267	26	specific	specific	JJ
cord-328460-thx9zh11	267	27	adsorption	adsorption	NN
cord-328460-thx9zh11	267	28	that	that	WDT
cord-328460-thx9zh11	267	29	can	can	MD
cord-328460-thx9zh11	267	30	affect	affect	VB
cord-328460-thx9zh11	267	31	the	the	DT
cord-328460-thx9zh11	267	32	amplification	amplification	NN
cord-328460-thx9zh11	267	33	signal	signal	NN
cord-328460-thx9zh11	267	34	and	and	CC
cord-328460-thx9zh11	267	35	the	the	DT
cord-328460-thx9zh11	267	36	signal	signal	NN
cord-328460-thx9zh11	267	37	curve	curve	NN
cord-328460-thx9zh11	267	38	of	of	IN
cord-328460-thx9zh11	267	39	NASBA	NASBA	NNP
cord-328460-thx9zh11	267	40	assay	assay	NN
cord-328460-thx9zh11	267	41	.	.	.
cord-328460-thx9zh11	268	1	Isothermal	Isothermal	NNP
cord-328460-thx9zh11	268	2	DNA	dna	NN
cord-328460-thx9zh11	268	3	amplification	amplification	NN
cord-328460-thx9zh11	268	4	is	be	VBZ
cord-328460-thx9zh11	268	5	rapid	rapid	JJ
cord-328460-thx9zh11	268	6	,	,	,
cord-328460-thx9zh11	268	7	cost	cost	VBD
cord-328460-thx9zh11	268	8	effective	effective	JJ
cord-328460-thx9zh11	268	9	,	,	,
cord-328460-thx9zh11	268	10	easy	easy	JJ
cord-328460-thx9zh11	268	11	-	-	HYPH
cord-328460-thx9zh11	268	12	to	to	IN
cord-328460-thx9zh11	268	13	-	-	HYPH
cord-328460-thx9zh11	268	14	use	use	NN
cord-328460-thx9zh11	268	15	and	and	CC
cord-328460-thx9zh11	268	16	more	more	RBR
cord-328460-thx9zh11	268	17	tolerant	tolerant	JJ
cord-328460-thx9zh11	268	18	to	to	IN
cord-328460-thx9zh11	268	19	inhibitory	inhibitory	JJ
cord-328460-thx9zh11	268	20	components	component	NNS
cord-328460-thx9zh11	268	21	from	from	IN
cord-328460-thx9zh11	268	22	a	a	DT
cord-328460-thx9zh11	268	23	crude	crude	JJ
cord-328460-thx9zh11	268	24	sample	sample	NN
cord-328460-thx9zh11	268	25	compared	compare	VBN
cord-328460-thx9zh11	268	26	with	with	IN
cord-328460-thx9zh11	268	27	PCR	PCR	NNP
cord-328460-thx9zh11	268	28	,	,	,
cord-328460-thx9zh11	268	29	which	which	WDT
cord-328460-thx9zh11	268	30	greatly	greatly	RB
cord-328460-thx9zh11	268	31	simplifies	simplify	VBZ
cord-328460-thx9zh11	268	32	point	point	NN
cord-328460-thx9zh11	268	33	-	-	HYPH
cord-328460-thx9zh11	268	34	of	of	IN
cord-328460-thx9zh11	268	35	-	-	HYPH
cord-328460-thx9zh11	268	36	care	care	NN
cord-328460-thx9zh11	268	37	diagnosis	diagnosis	NN
cord-328460-thx9zh11	268	38	,	,	,
cord-328460-thx9zh11	268	39	but	but	CC
cord-328460-thx9zh11	268	40	at	at	IN
cord-328460-thx9zh11	268	41	the	the	DT
cord-328460-thx9zh11	268	42	same	same	JJ
cord-328460-thx9zh11	268	43	time	time	NN
cord-328460-thx9zh11	268	44	showing	show	VBG
cord-328460-thx9zh11	268	45	equivalent	equivalent	JJ
cord-328460-thx9zh11	268	46	or	or	CC
cord-328460-thx9zh11	268	47	higher	high	JJR
cord-328460-thx9zh11	268	48	sensitivity	sensitivity	NN
cord-328460-thx9zh11	268	49	and	and	CC
cord-328460-thx9zh11	268	50	reliability	reliability	NN
cord-328460-thx9zh11	268	51	in	in	IN
cord-328460-thx9zh11	268	52	clinical	clinical	JJ
cord-328460-thx9zh11	268	53	diagnosis	diagnosis	NN
cord-328460-thx9zh11	268	54	.	.	.
cord-328460-thx9zh11	269	1	The	the	DT
cord-328460-thx9zh11	269	2	use	use	NN
cord-328460-thx9zh11	269	3	of	of	IN
cord-328460-thx9zh11	269	4	fully	fully	RB
cord-328460-thx9zh11	269	5	enclosed	enclose	VBN
cord-328460-thx9zh11	269	6	microstructured	microstructured	JJ
cord-328460-thx9zh11	269	7	devices	device	NNS
cord-328460-thx9zh11	269	8	into	into	IN
cord-328460-thx9zh11	269	9	which	which	WDT
cord-328460-thx9zh11	269	10	performing	perform	VBG
cord-328460-thx9zh11	269	11	the	the	DT
cord-328460-thx9zh11	269	12	isothermal	isothermal	JJ
cord-328460-thx9zh11	269	13	amplification	amplification	NN
cord-328460-thx9zh11	269	14	reduces	reduce	VBZ
cord-328460-thx9zh11	269	15	the	the	DT
cord-328460-thx9zh11	269	16	risk	risk	NN
cord-328460-thx9zh11	269	17	of	of	IN
cord-328460-thx9zh11	269	18	sample	sample	NN
cord-328460-thx9zh11	269	19	contamination	contamination	NN
cord-328460-thx9zh11	269	20	and	and	CC
cord-328460-thx9zh11	269	21	allows	allow	VBZ
cord-328460-thx9zh11	269	22	integration	integration	NN
cord-328460-thx9zh11	269	23	and	and	CC
cord-328460-thx9zh11	269	24	portable	portable	JJ
cord-328460-thx9zh11	269	25	device	device	NN
cord-328460-thx9zh11	269	26	realization	realization	NN
cord-328460-thx9zh11	269	27	.	.	.
cord-328460-thx9zh11	270	1	Furthermore	furthermore	RB
cord-328460-thx9zh11	270	2	,	,	,
cord-328460-thx9zh11	270	3	the	the	DT
cord-328460-thx9zh11	270	4	implementation	implementation	NN
cord-328460-thx9zh11	270	5	of	of	IN
cord-328460-thx9zh11	270	6	isothermal	isothermal	JJ
cord-328460-thx9zh11	270	7	DNA	dna	NN
cord-328460-thx9zh11	270	8	amplification	amplification	NN
cord-328460-thx9zh11	270	9	in	in	IN
cord-328460-thx9zh11	270	10	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	270	11	systems	system	NNS
cord-328460-thx9zh11	270	12	has	have	VBZ
cord-328460-thx9zh11	270	13	the	the	DT
cord-328460-thx9zh11	270	14	added	add	VBN
cord-328460-thx9zh11	270	15	benefit	benefit	NN
cord-328460-thx9zh11	270	16	of	of	IN
cord-328460-thx9zh11	270	17	reduced	reduced	JJ
cord-328460-thx9zh11	270	18	reaction	reaction	NN
cord-328460-thx9zh11	270	19	volumes	volume	NNS
cord-328460-thx9zh11	270	20	that	that	WDT
cord-328460-thx9zh11	270	21	result	result	VBP
cord-328460-thx9zh11	270	22	in	in	IN
cord-328460-thx9zh11	270	23	a	a	DT
cord-328460-thx9zh11	270	24	decreased	decreased	JJ
cord-328460-thx9zh11	270	25	reagent	reagent	NN
cord-328460-thx9zh11	270	26	and	and	CC
cord-328460-thx9zh11	270	27	sample	sample	NN
cord-328460-thx9zh11	270	28	consumption	consumption	NN
cord-328460-thx9zh11	270	29	.	.	.
cord-328460-thx9zh11	271	1	The	the	DT
cord-328460-thx9zh11	271	2	possibility	possibility	NN
cord-328460-thx9zh11	271	3	of	of	IN
cord-328460-thx9zh11	271	4	lowering	lower	VBG
cord-328460-thx9zh11	271	5	sample	sample	NN
cord-328460-thx9zh11	271	6	requirement	requirement	NN
cord-328460-thx9zh11	271	7	constitutes	constitute	VBZ
cord-328460-thx9zh11	271	8	an	an	DT
cord-328460-thx9zh11	271	9	important	important	JJ
cord-328460-thx9zh11	271	10	usefulness	usefulness	NN
cord-328460-thx9zh11	271	11	,	,	,
cord-328460-thx9zh11	271	12	especially	especially	RB
cord-328460-thx9zh11	271	13	when	when	WRB
cord-328460-thx9zh11	271	14	limited	limited	JJ
cord-328460-thx9zh11	271	15	template	template	NN
cord-328460-thx9zh11	271	16	material	material	NN
cord-328460-thx9zh11	271	17	is	be	VBZ
cord-328460-thx9zh11	271	18	available	available	JJ
cord-328460-thx9zh11	271	19	.	.	.
cord-328460-thx9zh11	272	1	Reactions	reaction	NNS
cord-328460-thx9zh11	272	2	take	take	VBP
cord-328460-thx9zh11	272	3	place	place	NN
cord-328460-thx9zh11	272	4	in	in	IN
cord-328460-thx9zh11	272	5	a	a	DT
cord-328460-thx9zh11	272	6	microchamber	microchamber	NN
cord-328460-thx9zh11	272	7	or	or	CC
cord-328460-thx9zh11	272	8	in	in	IN
cord-328460-thx9zh11	272	9	a	a	DT
cord-328460-thx9zh11	272	10	microchannel	microchannel	NN
cord-328460-thx9zh11	272	11	;	;	:
cord-328460-thx9zh11	272	12	reservoirs	reservoir	NNS
cord-328460-thx9zh11	272	13	can	can	MD
cord-328460-thx9zh11	272	14	be	be	VB
cord-328460-thx9zh11	272	15	included	include	VBN
cord-328460-thx9zh11	272	16	to	to	TO
cord-328460-thx9zh11	272	17	inject	inject	VB
cord-328460-thx9zh11	272	18	reagents	reagent	NNS
cord-328460-thx9zh11	272	19	necessary	necessary	JJ
cord-328460-thx9zh11	272	20	for	for	IN
cord-328460-thx9zh11	272	21	reactions	reaction	NNS
cord-328460-thx9zh11	272	22	and	and	CC
cord-328460-thx9zh11	272	23	to	to	TO
cord-328460-thx9zh11	272	24	mix	mix	VB
cord-328460-thx9zh11	272	25	them	-PRON-	PRP
cord-328460-thx9zh11	272	26	with	with	IN
cord-328460-thx9zh11	272	27	the	the	DT
cord-328460-thx9zh11	272	28	sample	sample	NN
cord-328460-thx9zh11	272	29	.	.	.
cord-328460-thx9zh11	273	1	Primers	primer	NNS
cord-328460-thx9zh11	273	2	,	,	,
cord-328460-thx9zh11	273	3	enzymes	enzyme	NNS
cord-328460-thx9zh11	273	4	and	and	CC
cord-328460-thx9zh11	273	5	reagents	reagent	NNS
cord-328460-thx9zh11	273	6	can	can	MD
cord-328460-thx9zh11	273	7	also	also	RB
cord-328460-thx9zh11	273	8	be	be	VB
cord-328460-thx9zh11	273	9	stored	store	VBN
cord-328460-thx9zh11	273	10	in	in	IN
cord-328460-thx9zh11	273	11	the	the	DT
cord-328460-thx9zh11	273	12	chip	chip	NN
cord-328460-thx9zh11	273	13	and	and	CC
cord-328460-thx9zh11	273	14	,	,	,
cord-328460-thx9zh11	273	15	in	in	IN
cord-328460-thx9zh11	273	16	order	order	NN
cord-328460-thx9zh11	273	17	to	to	TO
cord-328460-thx9zh11	273	18	obtain	obtain	VB
cord-328460-thx9zh11	273	19	multiplex	multiplex	JJ
cord-328460-thx9zh11	273	20	DNA	dna	NN
cord-328460-thx9zh11	273	21	analyses	analysis	NNS
cord-328460-thx9zh11	273	22	,	,	,
cord-328460-thx9zh11	273	23	different	different	JJ
cord-328460-thx9zh11	273	24	primers	primer	NNS
cord-328460-thx9zh11	273	25	for	for	IN
cord-328460-thx9zh11	273	26	several	several	JJ
cord-328460-thx9zh11	273	27	targets	target	NNS
cord-328460-thx9zh11	273	28	can	can	MD
cord-328460-thx9zh11	273	29	be	be	VB
cord-328460-thx9zh11	273	30	employed	employ	VBN
cord-328460-thx9zh11	273	31	.	.	.
cord-328460-thx9zh11	274	1	The	the	DT
cord-328460-thx9zh11	274	2	described	describe	VBN
cord-328460-thx9zh11	274	3	approaches	approach	NNS
cord-328460-thx9zh11	274	4	differ	differ	VBP
cord-328460-thx9zh11	274	5	from	from	IN
cord-328460-thx9zh11	274	6	each	each	DT
cord-328460-thx9zh11	274	7	other	other	JJ
cord-328460-thx9zh11	274	8	in	in	IN
cord-328460-thx9zh11	274	9	terms	term	NNS
cord-328460-thx9zh11	274	10	of	of	IN
cord-328460-thx9zh11	274	11	operating	operating	NN
cord-328460-thx9zh11	274	12	temperature	temperature	NN
cord-328460-thx9zh11	274	13	,	,	,
cord-328460-thx9zh11	274	14	diagnostic	diagnostic	JJ
cord-328460-thx9zh11	274	15	application	application	NN
cord-328460-thx9zh11	274	16	,	,	,
cord-328460-thx9zh11	274	17	mechanism	mechanism	NN
cord-328460-thx9zh11	274	18	,	,	,
cord-328460-thx9zh11	274	19	strengths	strength	NNS
cord-328460-thx9zh11	274	20	and	and	CC
cord-328460-thx9zh11	274	21	weaknesses	weakness	NNS
cord-328460-thx9zh11	274	22	.	.	.
cord-328460-thx9zh11	275	1	From	from	IN
cord-328460-thx9zh11	275	2	this	this	DT
cord-328460-thx9zh11	275	3	perspective	perspective	NN
cord-328460-thx9zh11	275	4	,	,	,
cord-328460-thx9zh11	275	5	it	-PRON-	PRP
cord-328460-thx9zh11	275	6	is	be	VBZ
cord-328460-thx9zh11	275	7	worthy	worthy	JJ
cord-328460-thx9zh11	275	8	of	of	IN
cord-328460-thx9zh11	275	9	noting	note	VBG
cord-328460-thx9zh11	275	10	that	that	IN
cord-328460-thx9zh11	275	11	NASBA	NASBA	NNP
cord-328460-thx9zh11	275	12	,	,	,
cord-328460-thx9zh11	275	13	RCA	RCA	NNP
cord-328460-thx9zh11	275	14	,	,	,
cord-328460-thx9zh11	275	15	and	and	CC
cord-328460-thx9zh11	275	16	RPA	RPA	NNP
cord-328460-thx9zh11	275	17	reactions	reaction	NNS
cord-328460-thx9zh11	275	18	allow	allow	VBP
cord-328460-thx9zh11	275	19	nucleic	nucleic	JJ
cord-328460-thx9zh11	275	20	acid	acid	NN
cord-328460-thx9zh11	275	21	amplification	amplification	NN
cord-328460-thx9zh11	275	22	at	at	IN
cord-328460-thx9zh11	275	23	low	low	JJ
cord-328460-thx9zh11	275	24	temperature	temperature	NN
cord-328460-thx9zh11	275	25	,	,	,
cord-328460-thx9zh11	275	26	thus	thus	RB
cord-328460-thx9zh11	275	27	requiring	require	VBG
cord-328460-thx9zh11	275	28	less	less	JJR
cord-328460-thx9zh11	275	29	energy	energy	NN
cord-328460-thx9zh11	275	30	to	to	TO
cord-328460-thx9zh11	275	31	operate	operate	VB
cord-328460-thx9zh11	275	32	than	than	IN
cord-328460-thx9zh11	275	33	higher	high	JJR
cord-328460-thx9zh11	275	34	temperature	temperature	NN
cord-328460-thx9zh11	275	35	isothermal	isothermal	JJ
cord-328460-thx9zh11	275	36	amplification	amplification	NN
cord-328460-thx9zh11	275	37	(	(	-LRB-
cord-328460-thx9zh11	275	38	i.e.	i.e.	FW
cord-328460-thx9zh11	275	39	,	,	,
cord-328460-thx9zh11	275	40	LAMP	lamp	NN
cord-328460-thx9zh11	275	41	and	and	CC
cord-328460-thx9zh11	275	42	HDA	HDA	NNP
cord-328460-thx9zh11	275	43	)	)	-RRB-
cord-328460-thx9zh11	275	44	.	.	.
cord-328460-thx9zh11	276	1	Many	many	JJ
cord-328460-thx9zh11	276	2	miniaturized	miniaturize	VBD
cord-328460-thx9zh11	276	3	isothermal	isothermal	JJ
cord-328460-thx9zh11	276	4	systems	system	NNS
cord-328460-thx9zh11	276	5	exploit	exploit	VBP
cord-328460-thx9zh11	276	6	the	the	DT
cord-328460-thx9zh11	276	7	strand	strand	NN
cord-328460-thx9zh11	276	8	-	-	HYPH
cord-328460-thx9zh11	276	9	displacement	displacement	NN
cord-328460-thx9zh11	276	10	activity	activity	NN
cord-328460-thx9zh11	276	11	of	of	IN
cord-328460-thx9zh11	276	12	a	a	DT
cord-328460-thx9zh11	276	13	DNA	dna	NN
cord-328460-thx9zh11	276	14	polymerase	polymerase	NN
cord-328460-thx9zh11	276	15	(	(	-LRB-
cord-328460-thx9zh11	276	16	LAMP	LAMP	NNP
cord-328460-thx9zh11	276	17	,	,	,
cord-328460-thx9zh11	276	18	RCA	RCA	NNP
cord-328460-thx9zh11	276	19	,	,	,
cord-328460-thx9zh11	276	20	RPA	RPA	NNP
cord-328460-thx9zh11	276	21	,	,	,
cord-328460-thx9zh11	276	22	MDA	MDA	NNP
cord-328460-thx9zh11	276	23	)	)	-RRB-
cord-328460-thx9zh11	276	24	to	to	TO
cord-328460-thx9zh11	276	25	cyclically	cyclically	RB
cord-328460-thx9zh11	276	26	amplify	amplify	VB
cord-328460-thx9zh11	276	27	a	a	DT
cord-328460-thx9zh11	276	28	target	target	NN
cord-328460-thx9zh11	276	29	in	in	IN
cord-328460-thx9zh11	276	30	less	less	JJR
cord-328460-thx9zh11	276	31	than	than	IN
cord-328460-thx9zh11	276	32	an	an	DT
cord-328460-thx9zh11	276	33	hour	hour	NN
cord-328460-thx9zh11	276	34	.	.	.
cord-328460-thx9zh11	277	1	Target	target	NN
cord-328460-thx9zh11	277	2	application	application	NN
cord-328460-thx9zh11	277	3	can	can	MD
cord-328460-thx9zh11	277	4	vary	vary	VB
cord-328460-thx9zh11	277	5	from	from	IN
cord-328460-thx9zh11	277	6	single	single	JJ
cord-328460-thx9zh11	277	7	stranded	strand	VBN
cord-328460-thx9zh11	277	8	DNA	DNA	NNP
cord-328460-thx9zh11	277	9	or	or	CC
cord-328460-thx9zh11	277	10	RNA	RNA	NNP
cord-328460-thx9zh11	277	11	molecules	molecule	NNS
cord-328460-thx9zh11	277	12	to	to	TO
cord-328460-thx9zh11	277	13	double	double	VB
cord-328460-thx9zh11	277	14	stranded	strand	VBN
cord-328460-thx9zh11	277	15	DNA	dna	NN
cord-328460-thx9zh11	277	16	samples	sample	NNS
cord-328460-thx9zh11	277	17	.	.	.
cord-328460-thx9zh11	278	1	DNA	DNA	NNP
cord-328460-thx9zh11	278	2	is	be	VBZ
cord-328460-thx9zh11	278	3	often	often	RB
cord-328460-thx9zh11	278	4	preferred	prefer	VBN
cord-328460-thx9zh11	278	5	,	,	,
cord-328460-thx9zh11	278	6	where	where	WRB
cord-328460-thx9zh11	278	7	appropriate	appropriate	JJ
cord-328460-thx9zh11	278	8	,	,	,
cord-328460-thx9zh11	278	9	because	because	IN
cord-328460-thx9zh11	278	10	it	-PRON-	PRP
cord-328460-thx9zh11	278	11	is	be	VBZ
cord-328460-thx9zh11	278	12	more	more	RBR
cord-328460-thx9zh11	278	13	stable	stable	JJ
cord-328460-thx9zh11	278	14	than	than	IN
cord-328460-thx9zh11	278	15	RNA	RNA	NNP
cord-328460-thx9zh11	278	16	and	and	CC
cord-328460-thx9zh11	278	17	will	will	MD
cord-328460-thx9zh11	278	18	be	be	VB
cord-328460-thx9zh11	278	19	likely	likely	RB
cord-328460-thx9zh11	278	20	associated	associate	VBN
cord-328460-thx9zh11	278	21	with	with	IN
cord-328460-thx9zh11	278	22	a	a	DT
cord-328460-thx9zh11	278	23	higher	high	JJR
cord-328460-thx9zh11	278	24	sensitivity	sensitivity	NN
cord-328460-thx9zh11	278	25	in	in	IN
cord-328460-thx9zh11	278	26	samples	sample	NNS
cord-328460-thx9zh11	278	27	stored	store	VBN
cord-328460-thx9zh11	278	28	or	or	CC
cord-328460-thx9zh11	278	29	transported	transport	VBN
cord-328460-thx9zh11	278	30	in	in	IN
cord-328460-thx9zh11	278	31	suboptimal	suboptimal	JJ
cord-328460-thx9zh11	278	32	conditions	condition	NNS
cord-328460-thx9zh11	278	33	.	.	.
cord-328460-thx9zh11	279	1	A	a	DT
cord-328460-thx9zh11	279	2	summary	summary	NN
cord-328460-thx9zh11	279	3	of	of	IN
cord-328460-thx9zh11	279	4	isothermal	isothermal	JJ
cord-328460-thx9zh11	279	5	nucleic	nucleic	JJ
cord-328460-thx9zh11	279	6	acid	acid	NN
cord-328460-thx9zh11	279	7	amplification	amplification	NN
cord-328460-thx9zh11	279	8	methods	method	NNS
cord-328460-thx9zh11	279	9	here	here	RB
cord-328460-thx9zh11	279	10	described	describe	VBN
cord-328460-thx9zh11	279	11	is	be	VBZ
cord-328460-thx9zh11	279	12	provided	provide	VBN
cord-328460-thx9zh11	279	13	in	in	IN
cord-328460-thx9zh11	279	14	Table	Table	NNP
cord-328460-thx9zh11	279	15	1	1	CD
cord-328460-thx9zh11	279	16	.	.	.
cord-328460-thx9zh11	280	1	It	-PRON-	PRP
cord-328460-thx9zh11	280	2	should	should	MD
cord-328460-thx9zh11	280	3	be	be	VB
cord-328460-thx9zh11	280	4	noted	note	VBN
cord-328460-thx9zh11	280	5	that	that	IN
cord-328460-thx9zh11	280	6	the	the	DT
cord-328460-thx9zh11	280	7	choice	choice	NN
cord-328460-thx9zh11	280	8	of	of	IN
cord-328460-thx9zh11	280	9	the	the	DT
cord-328460-thx9zh11	280	10	method	method	NN
cord-328460-thx9zh11	280	11	is	be	VBZ
cord-328460-thx9zh11	280	12	strictly	strictly	RB
cord-328460-thx9zh11	280	13	related	related	JJ
cord-328460-thx9zh11	280	14	to	to	IN
cord-328460-thx9zh11	280	15	the	the	DT
cord-328460-thx9zh11	280	16	target	target	NN
cord-328460-thx9zh11	280	17	.	.	.
cord-328460-thx9zh11	281	1	NASBA	NASBA	NNP
cord-328460-thx9zh11	282	1	for	for	IN
cord-328460-thx9zh11	282	2	example	example	NN
cord-328460-thx9zh11	282	3	is	be	VBZ
cord-328460-thx9zh11	282	4	especially	especially	RB
cord-328460-thx9zh11	282	5	suited	suit	VBN
cord-328460-thx9zh11	282	6	for	for	IN
cord-328460-thx9zh11	282	7	RNAs	rna	NNS
cord-328460-thx9zh11	282	8	such	such	JJ
cord-328460-thx9zh11	282	9	as	as	IN
cord-328460-thx9zh11	282	10	mRNA	mRNA	NNP
cord-328460-thx9zh11	282	11	,	,	,
cord-328460-thx9zh11	282	12	rRNA	rRNA	NNP
cord-328460-thx9zh11	282	13	,	,	,
cord-328460-thx9zh11	282	14	tmRNA	tmrna	NN
cord-328460-thx9zh11	282	15	or	or	CC
cord-328460-thx9zh11	282	16	genomic	genomic	JJ
cord-328460-thx9zh11	282	17	RNA	RNA	NNP
cord-328460-thx9zh11	282	18	,	,	,
cord-328460-thx9zh11	282	19	while	while	IN
cord-328460-thx9zh11	282	20	it	-PRON-	PRP
cord-328460-thx9zh11	282	21	can	can	MD
cord-328460-thx9zh11	282	22	not	not	RB
cord-328460-thx9zh11	282	23	amplify	amplify	VB
cord-328460-thx9zh11	282	24	double	double	RB
cord-328460-thx9zh11	282	25	-	-	HYPH
cord-328460-thx9zh11	282	26	stranded	strand	VBN
cord-328460-thx9zh11	282	27	DNAs	dna	NNS
cord-328460-thx9zh11	282	28	unless	unless	IN
cord-328460-thx9zh11	282	29	subjected	subject	VBN
cord-328460-thx9zh11	282	30	to	to	IN
cord-328460-thx9zh11	282	31	a	a	DT
cord-328460-thx9zh11	282	32	denaturation	denaturation	NN
cord-328460-thx9zh11	282	33	step	step	NN
cord-328460-thx9zh11	282	34	.	.	.
cord-328460-thx9zh11	283	1	On	on	IN
cord-328460-thx9zh11	283	2	the	the	DT
cord-328460-thx9zh11	283	3	other	other	JJ
cord-328460-thx9zh11	283	4	hand	hand	NN
cord-328460-thx9zh11	283	5	,	,	,
cord-328460-thx9zh11	283	6	NASBA	NASBA	NNP
cord-328460-thx9zh11	283	7	is	be	VBZ
cord-328460-thx9zh11	283	8	not	not	RB
cord-328460-thx9zh11	283	9	free	free	JJ
cord-328460-thx9zh11	283	10	from	from	IN
cord-328460-thx9zh11	283	11	drawbacks	drawback	NNS
cord-328460-thx9zh11	283	12	,	,	,
cord-328460-thx9zh11	283	13	since	since	IN
cord-328460-thx9zh11	283	14	it	-PRON-	PRP
cord-328460-thx9zh11	283	15	is	be	VBZ
cord-328460-thx9zh11	283	16	prone	prone	JJ
cord-328460-thx9zh11	283	17	to	to	IN
cord-328460-thx9zh11	283	18	ribonuclease	ribonuclease	NN
cord-328460-thx9zh11	283	19	(	(	-LRB-
cord-328460-thx9zh11	283	20	RNase	rnase	NN
cord-328460-thx9zh11	283	21	)	)	-RRB-
cord-328460-thx9zh11	283	22	contamination	contamination	NN
cord-328460-thx9zh11	283	23	and	and	CC
cord-328460-thx9zh11	283	24	non	non	JJ
cord-328460-thx9zh11	283	25	-	-	JJ
cord-328460-thx9zh11	283	26	specific	specific	JJ
cord-328460-thx9zh11	283	27	adsorption	adsorption	NN
cord-328460-thx9zh11	283	28	that	that	WDT
cord-328460-thx9zh11	283	29	can	can	MD
cord-328460-thx9zh11	283	30	affect	affect	VB
cord-328460-thx9zh11	283	31	the	the	DT
cord-328460-thx9zh11	283	32	amplification	amplification	NN
cord-328460-thx9zh11	283	33	signal	signal	NN
cord-328460-thx9zh11	283	34	and	and	CC
cord-328460-thx9zh11	283	35	the	the	DT
cord-328460-thx9zh11	283	36	signal	signal	NN
cord-328460-thx9zh11	283	37	curve	curve	NN
cord-328460-thx9zh11	283	38	of	of	IN
cord-328460-thx9zh11	283	39	NASBA	NASBA	NNP
cord-328460-thx9zh11	283	40	assay	assay	NN
cord-328460-thx9zh11	283	41	.	.	.
cord-328460-thx9zh11	284	1	Contrary	contrary	JJ
cord-328460-thx9zh11	284	2	to	to	IN
cord-328460-thx9zh11	284	3	NASBA	NASBA	NNP
cord-328460-thx9zh11	284	4	,	,	,
cord-328460-thx9zh11	284	5	LAMP	LAMP	NNP
cord-328460-thx9zh11	284	6	enables	enable	VBZ
cord-328460-thx9zh11	284	7	duplex	duplex	JJ
cord-328460-thx9zh11	284	8	DNA	dna	NN
cord-328460-thx9zh11	284	9	amplification	amplification	NN
cord-328460-thx9zh11	284	10	because	because	IN
cord-328460-thx9zh11	284	11	,	,	,
cord-328460-thx9zh11	284	12	in	in	IN
cord-328460-thx9zh11	284	13	the	the	DT
cord-328460-thx9zh11	284	14	condition	condition	NN
cord-328460-thx9zh11	284	15	of	of	IN
cord-328460-thx9zh11	284	16	dynamic	dynamic	JJ
cord-328460-thx9zh11	284	17	equilibrium	equilibrium	NN
cord-328460-thx9zh11	284	18	at	at	IN
cord-328460-thx9zh11	284	19	65	65	CD
cord-328460-thx9zh11	284	20	°	°	NN
cord-328460-thx9zh11	284	21	C	c	NN
cord-328460-thx9zh11	284	22	,	,	,
cord-328460-thx9zh11	284	23	one	one	CD
cord-328460-thx9zh11	284	24	of	of	IN
cord-328460-thx9zh11	284	25	the	the	DT
cord-328460-thx9zh11	284	26	LAMP	LAMP	NNP
cord-328460-thx9zh11	284	27	primers	primer	NNS
cord-328460-thx9zh11	284	28	can	can	MD
cord-328460-thx9zh11	284	29	anneal	anneal	VB
cord-328460-thx9zh11	284	30	to	to	IN
cord-328460-thx9zh11	284	31	the	the	DT
cord-328460-thx9zh11	284	32	complimentary	complimentary	JJ
cord-328460-thx9zh11	284	33	sequence	sequence	NN
cord-328460-thx9zh11	284	34	of	of	IN
cord-328460-thx9zh11	284	35	double	double	RB
cord-328460-thx9zh11	284	36	-	-	HYPH
cord-328460-thx9zh11	284	37	stranded	strand	VBN
cord-328460-thx9zh11	284	38	DNA	dna	NN
cord-328460-thx9zh11	284	39	without	without	IN
cord-328460-thx9zh11	284	40	any	any	DT
cord-328460-thx9zh11	284	41	initial	initial	JJ
cord-328460-thx9zh11	284	42	denaturation	denaturation	NN
cord-328460-thx9zh11	284	43	step	step	NN
cord-328460-thx9zh11	284	44	.	.	.
cord-328460-thx9zh11	285	1	LAMP	LAMP	NNP
cord-328460-thx9zh11	285	2	can	can	MD
cord-328460-thx9zh11	285	3	also	also	RB
cord-328460-thx9zh11	285	4	be	be	VB
cord-328460-thx9zh11	285	5	applied	apply	VBN
cord-328460-thx9zh11	285	6	to	to	IN
cord-328460-thx9zh11	285	7	the	the	DT
cord-328460-thx9zh11	285	8	detection	detection	NN
cord-328460-thx9zh11	285	9	of	of	IN
cord-328460-thx9zh11	285	10	RNA	RNA	NNP
cord-328460-thx9zh11	285	11	with	with	IN
cord-328460-thx9zh11	285	12	the	the	DT
cord-328460-thx9zh11	285	13	addition	addition	NN
cord-328460-thx9zh11	285	14	of	of	IN
cord-328460-thx9zh11	285	15	reverse	reverse	JJ
cord-328460-thx9zh11	285	16	transcriptase	transcriptase	NN
cord-328460-thx9zh11	285	17	.	.	.
cord-328460-thx9zh11	286	1	Another	another	DT
cord-328460-thx9zh11	286	2	important	important	JJ
cord-328460-thx9zh11	286	3	advantage	advantage	NN
cord-328460-thx9zh11	286	4	of	of	IN
cord-328460-thx9zh11	286	5	the	the	DT
cord-328460-thx9zh11	286	6	LAMP	LAMP	NNP
cord-328460-thx9zh11	286	7	method	method	NN
cord-328460-thx9zh11	286	8	is	be	VBZ
cord-328460-thx9zh11	286	9	its	-PRON-	PRP$
cord-328460-thx9zh11	286	10	simplicity	simplicity	NN
cord-328460-thx9zh11	286	11	for	for	IN
cord-328460-thx9zh11	286	12	detection	detection	NN
cord-328460-thx9zh11	286	13	of	of	IN
cord-328460-thx9zh11	286	14	the	the	DT
cord-328460-thx9zh11	286	15	amplification	amplification	NN
cord-328460-thx9zh11	286	16	reaction	reaction	NN
cord-328460-thx9zh11	286	17	,	,	,
cord-328460-thx9zh11	286	18	due	due	IN
cord-328460-thx9zh11	286	19	to	to	IN
cord-328460-thx9zh11	286	20	the	the	DT
cord-328460-thx9zh11	286	21	insoluble	insoluble	JJ
cord-328460-thx9zh11	286	22	pyrophosphate	pyrophosphate	NN
cord-328460-thx9zh11	286	23	byproduct	byproduct	NN
cord-328460-thx9zh11	286	24	that	that	WDT
cord-328460-thx9zh11	286	25	can	can	MD
cord-328460-thx9zh11	286	26	be	be	VB
cord-328460-thx9zh11	286	27	easily	easily	RB
cord-328460-thx9zh11	286	28	quantified	quantify	VBN
cord-328460-thx9zh11	286	29	turbimetrically	turbimetrically	RB
cord-328460-thx9zh11	286	30	.	.	.
cord-328460-thx9zh11	287	1	In	in	IN
cord-328460-thx9zh11	287	2	a	a	DT
cord-328460-thx9zh11	287	3	different	different	JJ
cord-328460-thx9zh11	287	4	manner	manner	NN
cord-328460-thx9zh11	287	5	,	,	,
cord-328460-thx9zh11	287	6	the	the	DT
cord-328460-thx9zh11	287	7	HDA	hda	NN
cord-328460-thx9zh11	287	8	reaction	reaction	NN
cord-328460-thx9zh11	287	9	exploits	exploit	VBZ
cord-328460-thx9zh11	287	10	the	the	DT
cord-328460-thx9zh11	287	11	activity	activity	NN
cord-328460-thx9zh11	287	12	of	of	IN
cord-328460-thx9zh11	287	13	a	a	DT
cord-328460-thx9zh11	287	14	DNA	dna	NN
cord-328460-thx9zh11	287	15	helicase	helicase	NN
cord-328460-thx9zh11	287	16	in	in	IN
cord-328460-thx9zh11	287	17	combination	combination	NN
cord-328460-thx9zh11	287	18	with	with	IN
cord-328460-thx9zh11	287	19	other	other	JJ
cord-328460-thx9zh11	287	20	accessory	accessory	NN
cord-328460-thx9zh11	287	21	proteins	protein	NNS
cord-328460-thx9zh11	287	22	to	to	TO
cord-328460-thx9zh11	287	23	separate	separate	VB
cord-328460-thx9zh11	287	24	complementary	complementary	JJ
cord-328460-thx9zh11	287	25	strands	strand	NNS
cord-328460-thx9zh11	287	26	of	of	IN
cord-328460-thx9zh11	287	27	ds	ds	NNP
cord-328460-thx9zh11	287	28	DNAs	DNAs	NNP
cord-328460-thx9zh11	287	29	,	,	,
cord-328460-thx9zh11	287	30	thus	thus	RB
cord-328460-thx9zh11	287	31	mimicking	mimic	VBG
cord-328460-thx9zh11	287	32	the	the	DT
cord-328460-thx9zh11	287	33	denaturation	denaturation	NN
cord-328460-thx9zh11	287	34	mechanism	mechanism	NN
cord-328460-thx9zh11	287	35	in	in	IN
cord-328460-thx9zh11	287	36	living	live	VBG
cord-328460-thx9zh11	287	37	organisms	organism	NNS
cord-328460-thx9zh11	287	38	.	.	.
cord-328460-thx9zh11	288	1	However	however	RB
cord-328460-thx9zh11	288	2	HDA	HDA	NNP
cord-328460-thx9zh11	288	3	suffers	suffer	VBZ
cord-328460-thx9zh11	288	4	from	from	IN
cord-328460-thx9zh11	288	5	the	the	DT
cord-328460-thx9zh11	288	6	limited	limited	JJ
cord-328460-thx9zh11	288	7	speed	speed	NN
cord-328460-thx9zh11	288	8	and	and	CC
cord-328460-thx9zh11	288	9	processivity	processivity	NN
cord-328460-thx9zh11	288	10	of	of	IN
cord-328460-thx9zh11	288	11	UvrD	UvrD	NNP
cord-328460-thx9zh11	288	12	helicase	helicase	NN
cord-328460-thx9zh11	288	13	that	that	WDT
cord-328460-thx9zh11	288	14	is	be	VBZ
cord-328460-thx9zh11	288	15	able	able	JJ
cord-328460-thx9zh11	288	16	to	to	TO
cord-328460-thx9zh11	288	17	amplify	amplify	VB
cord-328460-thx9zh11	288	18	only	only	JJ
cord-328460-thx9zh11	288	19	short	short	JJ
cord-328460-thx9zh11	288	20	DNA	dna	NN
cord-328460-thx9zh11	288	21	sequences	sequence	NNS
cord-328460-thx9zh11	288	22	(	(	-LRB-
cord-328460-thx9zh11	288	23	from	from	IN
cord-328460-thx9zh11	288	24	70	70	CD
cord-328460-thx9zh11	288	25	to	to	TO
cord-328460-thx9zh11	288	26	120	120	CD
cord-328460-thx9zh11	288	27	bp	bp	NNS
cord-328460-thx9zh11	288	28	)	)	-RRB-
cord-328460-thx9zh11	288	29	.	.	.
cord-328460-thx9zh11	289	1	As	as	IN
cord-328460-thx9zh11	289	2	an	an	DT
cord-328460-thx9zh11	289	3	attempt	attempt	NN
cord-328460-thx9zh11	289	4	to	to	TO
cord-328460-thx9zh11	289	5	circumvent	circumvent	VB
cord-328460-thx9zh11	289	6	this	this	DT
cord-328460-thx9zh11	289	7	drawback	drawback	NN
cord-328460-thx9zh11	289	8	,	,	,
cord-328460-thx9zh11	289	9	the	the	DT
cord-328460-thx9zh11	289	10	limited	limited	JJ
cord-328460-thx9zh11	289	11	capability	capability	NN
cord-328460-thx9zh11	289	12	of	of	IN
cord-328460-thx9zh11	289	13	UvrD	UvrD	NNP
cord-328460-thx9zh11	289	14	HDA	hda	NN
cord-328460-thx9zh11	289	15	system	system	NN
cord-328460-thx9zh11	289	16	in	in	IN
cord-328460-thx9zh11	289	17	efficiently	efficiently	RB
cord-328460-thx9zh11	289	18	amplifying	amplify	VBG
cord-328460-thx9zh11	289	19	long	long	JJ
cord-328460-thx9zh11	289	20	target	target	NN
cord-328460-thx9zh11	289	21	sequences	sequence	NNS
cord-328460-thx9zh11	289	22	was	be	VBD
cord-328460-thx9zh11	289	23	improved	improve	VBN
cord-328460-thx9zh11	289	24	by	by	IN
cord-328460-thx9zh11	289	25	using	use	VBG
cord-328460-thx9zh11	289	26	a	a	DT
cord-328460-thx9zh11	289	27	thermostable	thermostable	JJ
cord-328460-thx9zh11	289	28	UvrD	UvrD	NNP
cord-328460-thx9zh11	289	29	helicase	helicase	NN
cord-328460-thx9zh11	289	30	(	(	-LRB-
cord-328460-thx9zh11	289	31	Tte	Tte	NNP
cord-328460-thx9zh11	289	32	-	-	HYPH
cord-328460-thx9zh11	289	33	UvrD	UvrD	NNP
cord-328460-thx9zh11	289	34	)	)	-RRB-
cord-328460-thx9zh11	289	35	.	.	.
cord-328460-thx9zh11	290	1	The	the	DT
cord-328460-thx9zh11	290	2	target	target	NN
cord-328460-thx9zh11	290	3	applications	application	NNS
cord-328460-thx9zh11	290	4	of	of	IN
cord-328460-thx9zh11	290	5	the	the	DT
cord-328460-thx9zh11	290	6	RCA	RCA	NNP
cord-328460-thx9zh11	290	7	method	method	NN
cord-328460-thx9zh11	290	8	are	be	VBP
cord-328460-thx9zh11	290	9	wide	wide	JJ
cord-328460-thx9zh11	290	10	,	,	,
cord-328460-thx9zh11	290	11	ranging	range	VBG
cord-328460-thx9zh11	290	12	from	from	IN
cord-328460-thx9zh11	290	13	small	small	JJ
cord-328460-thx9zh11	290	14	and	and	CC
cord-328460-thx9zh11	290	15	circular	circular	JJ
cord-328460-thx9zh11	290	16	single	single	RB
cord-328460-thx9zh11	290	17	-	-	HYPH
cord-328460-thx9zh11	290	18	stranded	strand	VBN
cord-328460-thx9zh11	290	19	DNAs	dna	NNS
cord-328460-thx9zh11	290	20	to	to	TO
cord-328460-thx9zh11	290	21	double	double	VB
cord-328460-thx9zh11	290	22	stranded	strand	VBN
cord-328460-thx9zh11	290	23	linear	linear	JJ
cord-328460-thx9zh11	290	24	DNA	dna	NN
cord-328460-thx9zh11	290	25	molecules	molecule	NNS
cord-328460-thx9zh11	290	26	and	and	CC
cord-328460-thx9zh11	290	27	even	even	RB
cord-328460-thx9zh11	290	28	mRNA	mRNA	NNP
cord-328460-thx9zh11	290	29	.	.	.
cord-328460-thx9zh11	291	1	Sample	sample	JJ
cord-328460-thx9zh11	291	2	pre	pre	NNS
cord-328460-thx9zh11	291	3	-	-	NNS
cord-328460-thx9zh11	291	4	treatments	treatment	NNS
cord-328460-thx9zh11	291	5	are	be	VBP
cord-328460-thx9zh11	291	6	required	require	VBN
cord-328460-thx9zh11	291	7	before	before	IN
cord-328460-thx9zh11	291	8	padlock	padlock	NN
cord-328460-thx9zh11	291	9	probe	probe	NN
cord-328460-thx9zh11	291	10	circularization	circularization	VBP
cord-328460-thx9zh11	291	11	in	in	IN
cord-328460-thx9zh11	291	12	order	order	NN
cord-328460-thx9zh11	291	13	to	to	TO
cord-328460-thx9zh11	291	14	allow	allow	VB
cord-328460-thx9zh11	291	15	the	the	DT
cord-328460-thx9zh11	291	16	transformation	transformation	NN
cord-328460-thx9zh11	291	17	of	of	IN
cord-328460-thx9zh11	291	18	duplex	duplex	JJ
cord-328460-thx9zh11	291	19	genomic	genomic	JJ
cord-328460-thx9zh11	291	20	DNA	dna	NN
cord-328460-thx9zh11	291	21	into	into	IN
cord-328460-thx9zh11	291	22	accessible	accessible	JJ
cord-328460-thx9zh11	291	23	single	single	RB
cord-328460-thx9zh11	291	24	-	-	HYPH
cord-328460-thx9zh11	291	25	stranded	strand	VBN
cord-328460-thx9zh11	291	26	DNA	dna	NN
cord-328460-thx9zh11	291	27	targets	target	NNS
cord-328460-thx9zh11	291	28	;	;	:
cord-328460-thx9zh11	291	29	this	this	DT
cord-328460-thx9zh11	291	30	additional	additional	JJ
cord-328460-thx9zh11	291	31	heating	heating	NN
cord-328460-thx9zh11	291	32	step	step	NN
cord-328460-thx9zh11	291	33	is	be	VBZ
cord-328460-thx9zh11	291	34	responsible	responsible	JJ
cord-328460-thx9zh11	291	35	for	for	IN
cord-328460-thx9zh11	291	36	increased	increase	VBN
cord-328460-thx9zh11	291	37	power	power	NN
cord-328460-thx9zh11	291	38	demand	demand	NN
cord-328460-thx9zh11	291	39	and	and	CC
cord-328460-thx9zh11	291	40	more	more	RBR
cord-328460-thx9zh11	291	41	complicated	complicated	JJ
cord-328460-thx9zh11	291	42	control	control	NN
cord-328460-thx9zh11	291	43	mechanisms	mechanism	NNS
cord-328460-thx9zh11	291	44	.	.	.
cord-328460-thx9zh11	292	1	In	in	IN
cord-328460-thx9zh11	292	2	mRNA	mRNA	NNP
cord-328460-thx9zh11	292	3	detection	detection	NN
cord-328460-thx9zh11	292	4	,	,	,
cord-328460-thx9zh11	292	5	instead	instead	RB
cord-328460-thx9zh11	292	6	,	,	,
cord-328460-thx9zh11	292	7	the	the	DT
cord-328460-thx9zh11	292	8	pre	pre	JJ
cord-328460-thx9zh11	292	9	-	-	JJ
cord-328460-thx9zh11	292	10	treatment	treatment	JJ
cord-328460-thx9zh11	292	11	step	step	NN
cord-328460-thx9zh11	292	12	consists	consist	VBZ
cord-328460-thx9zh11	292	13	of	of	IN
cord-328460-thx9zh11	292	14	cDNA	cdna	NN
cord-328460-thx9zh11	292	15	synthesis	synthesis	NN
cord-328460-thx9zh11	292	16	by	by	IN
cord-328460-thx9zh11	292	17	a	a	DT
cord-328460-thx9zh11	292	18	reverse	reverse	JJ
cord-328460-thx9zh11	292	19	-	-	HYPH
cord-328460-thx9zh11	292	20	transcription	transcription	NN
cord-328460-thx9zh11	292	21	followed	follow	VBN
cord-328460-thx9zh11	292	22	by	by	IN
cord-328460-thx9zh11	292	23	mRNA	mrna	NN
cord-328460-thx9zh11	292	24	degradation	degradation	NN
cord-328460-thx9zh11	292	25	by	by	IN
cord-328460-thx9zh11	292	26	RNase	rnase	NN
cord-328460-thx9zh11	292	27	H	h	NN
cord-328460-thx9zh11	292	28	digestion	digestion	NN
cord-328460-thx9zh11	292	29	.	.	.
cord-328460-thx9zh11	293	1	On	on	IN
cord-328460-thx9zh11	293	2	the	the	DT
cord-328460-thx9zh11	293	3	other	other	JJ
cord-328460-thx9zh11	293	4	hand	hand	NN
cord-328460-thx9zh11	293	5	,	,	,
cord-328460-thx9zh11	293	6	ss	ss	NNP
cord-328460-thx9zh11	293	7	the	the	DT
cord-328460-thx9zh11	293	8	circularizing	circularize	VBG
cord-328460-thx9zh11	293	9	step	step	NN
cord-328460-thx9zh11	293	10	of	of	IN
cord-328460-thx9zh11	293	11	a	a	DT
cord-328460-thx9zh11	293	12	padlock	padlock	NN
cord-328460-thx9zh11	293	13	probe	probe	NN
cord-328460-thx9zh11	293	14	is	be	VBZ
cord-328460-thx9zh11	293	15	strictly	strictly	RB
cord-328460-thx9zh11	293	16	target	target	NN
cord-328460-thx9zh11	293	17	-	-	HYPH
cord-328460-thx9zh11	293	18	dependent	dependent	JJ
cord-328460-thx9zh11	293	19	,	,	,
cord-328460-thx9zh11	293	20	high	high	JJ
cord-328460-thx9zh11	293	21	enough	enough	JJ
cord-328460-thx9zh11	293	22	specificity	specificity	NN
cord-328460-thx9zh11	293	23	is	be	VBZ
cord-328460-thx9zh11	293	24	ensured	ensure	VBN
cord-328460-thx9zh11	293	25	to	to	TO
cord-328460-thx9zh11	293	26	allow	allow	VB
cord-328460-thx9zh11	293	27	SNP	SNP	NNP
cord-328460-thx9zh11	293	28	analysis	analysis	NN
cord-328460-thx9zh11	293	29	.	.	.
cord-328460-thx9zh11	294	1	Another	another	DT
cord-328460-thx9zh11	294	2	relevant	relevant	JJ
cord-328460-thx9zh11	294	3	advantage	advantage	NN
cord-328460-thx9zh11	294	4	of	of	IN
cord-328460-thx9zh11	294	5	RCA	RCA	NNP
cord-328460-thx9zh11	294	6	is	be	VBZ
cord-328460-thx9zh11	294	7	its	-PRON-	PRP$
cord-328460-thx9zh11	294	8	capability	capability	NN
cord-328460-thx9zh11	294	9	to	to	TO
cord-328460-thx9zh11	294	10	detect	detect	VB
cord-328460-thx9zh11	294	11	miRNA	miRNA	NNP
cord-328460-thx9zh11	294	12	samples	sample	NNS
cord-328460-thx9zh11	294	13	,	,	,
cord-328460-thx9zh11	294	14	which	which	WDT
cord-328460-thx9zh11	294	15	arises	arise	VBZ
cord-328460-thx9zh11	294	16	from	from	IN
cord-328460-thx9zh11	294	17	its	-PRON-	PRP$
cord-328460-thx9zh11	294	18	inherent	inherent	JJ
cord-328460-thx9zh11	294	19	nature	nature	NN
cord-328460-thx9zh11	294	20	for	for	IN
cord-328460-thx9zh11	294	21	specific	specific	JJ
cord-328460-thx9zh11	294	22	detection	detection	NN
cord-328460-thx9zh11	294	23	of	of	IN
cord-328460-thx9zh11	294	24	short	short	JJ
cord-328460-thx9zh11	294	25	sequences	sequence	NNS
cord-328460-thx9zh11	294	26	.	.	.
cord-328460-thx9zh11	295	1	In	in	IN
cord-328460-thx9zh11	295	2	RPA	RPA	NNP
cord-328460-thx9zh11	295	3	,	,	,
cord-328460-thx9zh11	295	4	an	an	DT
cord-328460-thx9zh11	295	5	initial	initial	JJ
cord-328460-thx9zh11	295	6	denaturation	denaturation	NN
cord-328460-thx9zh11	295	7	step	step	NN
cord-328460-thx9zh11	295	8	is	be	VBZ
cord-328460-thx9zh11	295	9	not	not	RB
cord-328460-thx9zh11	295	10	required	require	VBN
cord-328460-thx9zh11	295	11	thanks	thank	NNS
cord-328460-thx9zh11	295	12	to	to	IN
cord-328460-thx9zh11	295	13	the	the	DT
cord-328460-thx9zh11	295	14	activity	activity	NN
cord-328460-thx9zh11	295	15	of	of	IN
cord-328460-thx9zh11	295	16	recombinase	recombinase	NN
cord-328460-thx9zh11	295	17	-	-	HYPH
cord-328460-thx9zh11	295	18	primer	primer	NN
cord-328460-thx9zh11	295	19	complexes	complex	NNS
cord-328460-thx9zh11	295	20	that	that	WDT
cord-328460-thx9zh11	295	21	scan	scan	VBP
cord-328460-thx9zh11	295	22	the	the	DT
cord-328460-thx9zh11	295	23	double	double	RB
cord-328460-thx9zh11	295	24	-	-	HYPH
cord-328460-thx9zh11	295	25	stranded	strand	VBN
cord-328460-thx9zh11	295	26	DNA	dna	NN
cord-328460-thx9zh11	295	27	by	by	IN
cord-328460-thx9zh11	295	28	promoting	promote	VBG
cord-328460-thx9zh11	295	29	the	the	DT
cord-328460-thx9zh11	295	30	primer	primer	NN
cord-328460-thx9zh11	295	31	binding	bind	VBG
cord-328460-thx9zh11	295	32	at	at	IN
cord-328460-thx9zh11	295	33	the	the	DT
cord-328460-thx9zh11	295	34	target	target	NN
cord-328460-thx9zh11	295	35	sequence	sequence	NN
cord-328460-thx9zh11	295	36	and	and	CC
cord-328460-thx9zh11	295	37	the	the	DT
cord-328460-thx9zh11	295	38	displacement	displacement	NN
cord-328460-thx9zh11	295	39	of	of	IN
cord-328460-thx9zh11	295	40	the	the	DT
cord-328460-thx9zh11	295	41	non	non	JJ
cord-328460-thx9zh11	295	42	-	-	JJ
cord-328460-thx9zh11	295	43	template	template	JJ
cord-328460-thx9zh11	295	44	strand	strand	NN
cord-328460-thx9zh11	295	45	.	.	.
cord-328460-thx9zh11	296	1	The	the	DT
cord-328460-thx9zh11	296	2	RPA	RPA	NNP
cord-328460-thx9zh11	296	3	reaction	reaction	NN
cord-328460-thx9zh11	296	4	has	have	VBZ
cord-328460-thx9zh11	296	5	the	the	DT
cord-328460-thx9zh11	296	6	ability	ability	NN
cord-328460-thx9zh11	296	7	to	to	TO
cord-328460-thx9zh11	296	8	proceed	proceed	VB
cord-328460-thx9zh11	296	9	at	at	IN
cord-328460-thx9zh11	296	10	a	a	DT
cord-328460-thx9zh11	296	11	variety	variety	NN
cord-328460-thx9zh11	296	12	of	of	IN
cord-328460-thx9zh11	296	13	temperatures	temperature	NNS
cord-328460-thx9zh11	296	14	since	since	IN
cord-328460-thx9zh11	296	15	it	-PRON-	PRP
cord-328460-thx9zh11	296	16	is	be	VBZ
cord-328460-thx9zh11	296	17	tolerant	tolerant	JJ
cord-328460-thx9zh11	296	18	to	to	IN
cord-328460-thx9zh11	296	19	fluctuations	fluctuation	NNS
cord-328460-thx9zh11	296	20	of	of	IN
cord-328460-thx9zh11	296	21	the	the	DT
cord-328460-thx9zh11	296	22	incubation	incubation	NN
cord-328460-thx9zh11	296	23	temperature	temperature	NN
cord-328460-thx9zh11	296	24	ranging	range	VBG
cord-328460-thx9zh11	296	25	from	from	IN
cord-328460-thx9zh11	296	26	37	37	CD
cord-328460-thx9zh11	296	27	°	°	NN
cord-328460-thx9zh11	296	28	C	c	NN
cord-328460-thx9zh11	296	29	to	to	IN
cord-328460-thx9zh11	296	30	42	42	CD
cord-328460-thx9zh11	296	31	°	°	NN
cord-328460-thx9zh11	296	32	C	c	NN
cord-328460-thx9zh11	296	33	.	.	.
cord-328460-thx9zh11	297	1	This	this	DT
cord-328460-thx9zh11	297	2	remarkable	remarkable	JJ
cord-328460-thx9zh11	297	3	property	property	NN
cord-328460-thx9zh11	297	4	of	of	IN
cord-328460-thx9zh11	297	5	RPA	RPA	NNP
cord-328460-thx9zh11	297	6	is	be	VBZ
cord-328460-thx9zh11	297	7	of	of	IN
cord-328460-thx9zh11	297	8	great	great	JJ
cord-328460-thx9zh11	297	9	appeal	appeal	NN
cord-328460-thx9zh11	297	10	for	for	IN
cord-328460-thx9zh11	297	11	field	field	NN
cord-328460-thx9zh11	297	12	applications	application	NNS
cord-328460-thx9zh11	297	13	where	where	WRB
cord-328460-thx9zh11	297	14	precise	precise	JJ
cord-328460-thx9zh11	297	15	temperature	temperature	NN
cord-328460-thx9zh11	297	16	control	control	NN
cord-328460-thx9zh11	297	17	is	be	VBZ
cord-328460-thx9zh11	297	18	often	often	RB
cord-328460-thx9zh11	297	19	technically	technically	RB
cord-328460-thx9zh11	297	20	challenging	challenging	JJ
cord-328460-thx9zh11	297	21	.	.	.
cord-328460-thx9zh11	298	1	MDA	MDA	NNP
cord-328460-thx9zh11	298	2	exploits	exploit	VBZ
cord-328460-thx9zh11	298	3	random	random	JJ
cord-328460-thx9zh11	298	4	exonuclease	exonuclease	NN
cord-328460-thx9zh11	298	5	-	-	HYPH
cord-328460-thx9zh11	298	6	resistant	resistant	JJ
cord-328460-thx9zh11	298	7	primers	primer	NNS
cord-328460-thx9zh11	298	8	and	and	CC
cord-328460-thx9zh11	298	9	the	the	DT
cord-328460-thx9zh11	298	10	activity	activity	NN
cord-328460-thx9zh11	298	11	of	of	IN
cord-328460-thx9zh11	298	12	φ29	φ29	NN
cord-328460-thx9zh11	298	13	DNA	dna	NN
cord-328460-thx9zh11	298	14	polymerase	polymerase	NN
cord-328460-thx9zh11	298	15	,	,	,
cord-328460-thx9zh11	298	16	already	already	RB
cord-328460-thx9zh11	298	17	used	use	VBN
cord-328460-thx9zh11	298	18	in	in	IN
cord-328460-thx9zh11	298	19	RCA	RCA	NNP
cord-328460-thx9zh11	298	20	,	,	,
cord-328460-thx9zh11	298	21	but	but	CC
cord-328460-thx9zh11	298	22	to	to	TO
cord-328460-thx9zh11	298	23	amplify	amplify	VB
cord-328460-thx9zh11	298	24	linear	linear	JJ
cord-328460-thx9zh11	298	25	genomic	genomic	JJ
cord-328460-thx9zh11	298	26	DNA	dna	NN
cord-328460-thx9zh11	298	27	rather	rather	RB
cord-328460-thx9zh11	298	28	than	than	IN
cord-328460-thx9zh11	298	29	for	for	IN
cord-328460-thx9zh11	298	30	circular	circular	JJ
cord-328460-thx9zh11	298	31	DNA	dna	NN
cord-328460-thx9zh11	298	32	amplification	amplification	NN
cord-328460-thx9zh11	298	33	.	.	.
cord-328460-thx9zh11	299	1	The	the	DT
cord-328460-thx9zh11	299	2	random	random	JJ
cord-328460-thx9zh11	299	3	priming	priming	NN
cord-328460-thx9zh11	299	4	on	on	IN
cord-328460-thx9zh11	299	5	the	the	DT
cord-328460-thx9zh11	299	6	double	double	RB
cord-328460-thx9zh11	299	7	-	-	HYPH
cord-328460-thx9zh11	299	8	stranded	strand	VBN
cord-328460-thx9zh11	299	9	DNA	dna	NN
cord-328460-thx9zh11	299	10	target	target	NN
cord-328460-thx9zh11	299	11	is	be	VBZ
cord-328460-thx9zh11	299	12	efficient	efficient	JJ
cord-328460-thx9zh11	299	13	enough	enough	RB
cord-328460-thx9zh11	299	14	to	to	TO
cord-328460-thx9zh11	299	15	obtain	obtain	VB
cord-328460-thx9zh11	299	16	an	an	DT
cord-328460-thx9zh11	299	17	initial	initial	JJ
cord-328460-thx9zh11	299	18	priming	priming	NN
cord-328460-thx9zh11	299	19	step	step	NN
cord-328460-thx9zh11	299	20	that	that	WDT
cord-328460-thx9zh11	299	21	is	be	VBZ
cord-328460-thx9zh11	299	22	followed	follow	VBN
cord-328460-thx9zh11	299	23	by	by	IN
cord-328460-thx9zh11	299	24	the	the	DT
cord-328460-thx9zh11	299	25	strand	strand	NN
cord-328460-thx9zh11	299	26	-	-	HYPH
cord-328460-thx9zh11	299	27	displacement	displacement	NN
cord-328460-thx9zh11	299	28	process	process	NN
cord-328460-thx9zh11	299	29	.	.	.
cord-328460-thx9zh11	300	1	The	the	DT
cord-328460-thx9zh11	300	2	MDA	MDA	NNP
cord-328460-thx9zh11	300	3	method	method	NN
cord-328460-thx9zh11	300	4	is	be	VBZ
cord-328460-thx9zh11	300	5	capable	capable	JJ
cord-328460-thx9zh11	300	6	of	of	IN
cord-328460-thx9zh11	300	7	producing	produce	VBG
cord-328460-thx9zh11	300	8	a	a	DT
cord-328460-thx9zh11	300	9	large	large	JJ
cord-328460-thx9zh11	300	10	number	number	NN
cord-328460-thx9zh11	300	11	of	of	IN
cord-328460-thx9zh11	300	12	amplification	amplification	NN
cord-328460-thx9zh11	300	13	products	product	NNS
cord-328460-thx9zh11	300	14	from	from	IN
cord-328460-thx9zh11	300	15	a	a	DT
cord-328460-thx9zh11	300	16	few	few	JJ
cord-328460-thx9zh11	300	17	DNA	dna	NN
cord-328460-thx9zh11	300	18	molecules	molecule	NNS
cord-328460-thx9zh11	300	19	regardless	regardless	RB
cord-328460-thx9zh11	300	20	of	of	IN
cord-328460-thx9zh11	300	21	the	the	DT
cord-328460-thx9zh11	300	22	type	type	NN
cord-328460-thx9zh11	300	23	of	of	IN
cord-328460-thx9zh11	300	24	clinical	clinical	JJ
cord-328460-thx9zh11	300	25	sample	sample	NN
cord-328460-thx9zh11	300	26	used	use	VBN
cord-328460-thx9zh11	300	27	including	include	VBG
cord-328460-thx9zh11	300	28	buccal	buccal	JJ
cord-328460-thx9zh11	300	29	swabs	swab	NNS
cord-328460-thx9zh11	300	30	,	,	,
cord-328460-thx9zh11	300	31	whole	whole	JJ
cord-328460-thx9zh11	300	32	blood	blood	NN
cord-328460-thx9zh11	300	33	,	,	,
cord-328460-thx9zh11	300	34	finger	finger	NN
cord-328460-thx9zh11	300	35	stick	stick	VB
cord-328460-thx9zh11	300	36	blood	blood	NN
cord-328460-thx9zh11	300	37	and	and	CC
cord-328460-thx9zh11	300	38	Guthrie	Guthrie	NNP
cord-328460-thx9zh11	300	39	cards	card	NNS
cord-328460-thx9zh11	300	40	.	.	.
cord-328460-thx9zh11	301	1	In	in	IN
cord-328460-thx9zh11	301	2	addition	addition	NN
cord-328460-thx9zh11	301	3	,	,	,
cord-328460-thx9zh11	301	4	the	the	DT
cord-328460-thx9zh11	301	5	use	use	NN
cord-328460-thx9zh11	301	6	of	of	IN
cord-328460-thx9zh11	301	7	a	a	DT
cord-328460-thx9zh11	301	8	high	high	JJ
cord-328460-thx9zh11	301	9	-	-	HYPH
cord-328460-thx9zh11	301	10	fidelity	fidelity	NN
cord-328460-thx9zh11	301	11	proofreading	proofreading	NN
cord-328460-thx9zh11	301	12	DNA	dna	NN
cord-328460-thx9zh11	301	13	polymerase	polymerase	NN
cord-328460-thx9zh11	301	14	ensures	ensure	VBZ
cord-328460-thx9zh11	301	15	a	a	DT
cord-328460-thx9zh11	301	16	significantly	significantly	RB
cord-328460-thx9zh11	301	17	lower	low	JJR
cord-328460-thx9zh11	301	18	error	error	NN
cord-328460-thx9zh11	301	19	rate	rate	NN
cord-328460-thx9zh11	301	20	during	during	IN
cord-328460-thx9zh11	301	21	the	the	DT
cord-328460-thx9zh11	301	22	amplification	amplification	NN
cord-328460-thx9zh11	301	23	reaction	reaction	NN
cord-328460-thx9zh11	301	24	and	and	CC
cord-328460-thx9zh11	301	25	preserves	preserve	VBZ
cord-328460-thx9zh11	301	26	DNA	dna	NN
cord-328460-thx9zh11	301	27	sequence	sequence	NN
cord-328460-thx9zh11	301	28	information	information	NN
cord-328460-thx9zh11	301	29	,	,	,
cord-328460-thx9zh11	301	30	an	an	DT
cord-328460-thx9zh11	301	31	advantage	advantage	NN
cord-328460-thx9zh11	301	32	to	to	TO
cord-328460-thx9zh11	301	33	consider	consider	VB
cord-328460-thx9zh11	301	34	when	when	WRB
cord-328460-thx9zh11	301	35	an	an	DT
cord-328460-thx9zh11	301	36	accurate	accurate	JJ
cord-328460-thx9zh11	301	37	genotyping	genotyping	NN
cord-328460-thx9zh11	301	38	is	be	VBZ
cord-328460-thx9zh11	301	39	required	require	VBN
cord-328460-thx9zh11	301	40	as	as	IN
cord-328460-thx9zh11	301	41	in	in	IN
cord-328460-thx9zh11	301	42	whole	whole	JJ
cord-328460-thx9zh11	301	43	genome	genome	NN
cord-328460-thx9zh11	301	44	amplification	amplification	NN
cord-328460-thx9zh11	301	45	(	(	-LRB-
cord-328460-thx9zh11	301	46	WGA	WGA	NNP
cord-328460-thx9zh11	301	47	)	)	-RRB-
cord-328460-thx9zh11	301	48	.	.	.
cord-328460-thx9zh11	302	1	The	the	DT
cord-328460-thx9zh11	302	2	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	302	3	lab	lab	NN
cord-328460-thx9zh11	302	4	-	-	HYPH
cord-328460-thx9zh11	302	5	on	on	IN
cord-328460-thx9zh11	302	6	-	-	HYPH
cord-328460-thx9zh11	302	7	a	a	DT
cord-328460-thx9zh11	302	8	-	-	HYPH
cord-328460-thx9zh11	302	9	chip	chip	NN
cord-328460-thx9zh11	302	10	technology	technology	NN
cord-328460-thx9zh11	302	11	for	for	IN
cord-328460-thx9zh11	302	12	the	the	DT
cord-328460-thx9zh11	302	13	amplification	amplification	NN
cord-328460-thx9zh11	302	14	of	of	IN
cord-328460-thx9zh11	302	15	nucleic	nucleic	JJ
cord-328460-thx9zh11	302	16	acids	acid	NNS
cord-328460-thx9zh11	302	17	offers	offer	VBZ
cord-328460-thx9zh11	302	18	great	great	JJ
cord-328460-thx9zh11	302	19	potential	potential	NN
cord-328460-thx9zh11	302	20	for	for	IN
cord-328460-thx9zh11	302	21	lower	low	JJR
cord-328460-thx9zh11	302	22	cost	cost	NN
cord-328460-thx9zh11	302	23	,	,	,
cord-328460-thx9zh11	302	24	higher	high	JJR
cord-328460-thx9zh11	302	25	speed	speed	NN
cord-328460-thx9zh11	302	26	,	,	,
cord-328460-thx9zh11	302	27	smaller	small	JJR
cord-328460-thx9zh11	302	28	sample	sample	NN
cord-328460-thx9zh11	302	29	consumption	consumption	NN
cord-328460-thx9zh11	302	30	and	and	CC
cord-328460-thx9zh11	302	31	automation	automation	NN
cord-328460-thx9zh11	302	32	of	of	IN
cord-328460-thx9zh11	302	33	all	all	DT
cord-328460-thx9zh11	302	34	processes	process	NNS
cord-328460-thx9zh11	302	35	from	from	IN
cord-328460-thx9zh11	302	36	sample	sample	NN
cord-328460-thx9zh11	302	37	preparation	preparation	NN
cord-328460-thx9zh11	302	38	to	to	TO
cord-328460-thx9zh11	302	39	signal	signal	VB
cord-328460-thx9zh11	302	40	detection	detection	NN
cord-328460-thx9zh11	302	41	.	.	.
cord-328460-thx9zh11	303	1	The	the	DT
cord-328460-thx9zh11	303	2	integration	integration	NN
cord-328460-thx9zh11	303	3	of	of	IN
cord-328460-thx9zh11	303	4	multiple	multiple	JJ
cord-328460-thx9zh11	303	5	functional	functional	JJ
cord-328460-thx9zh11	303	6	fluidic	fluidic	JJ
cord-328460-thx9zh11	303	7	modules	module	NNS
cord-328460-thx9zh11	303	8	enables	enable	VBZ
cord-328460-thx9zh11	303	9	the	the	DT
cord-328460-thx9zh11	303	10	analysis	analysis	NN
cord-328460-thx9zh11	303	11	of	of	IN
cord-328460-thx9zh11	303	12	smaller	small	JJR
cord-328460-thx9zh11	303	13	sample	sample	NN
cord-328460-thx9zh11	303	14	volumes	volume	NNS
cord-328460-thx9zh11	303	15	with	with	IN
cord-328460-thx9zh11	303	16	increased	increase	VBN
cord-328460-thx9zh11	303	17	reproducibility	reproducibility	NN
cord-328460-thx9zh11	303	18	,	,	,
cord-328460-thx9zh11	303	19	faster	fast	JJR
cord-328460-thx9zh11	303	20	analysis	analysis	NN
cord-328460-thx9zh11	303	21	and	and	CC
cord-328460-thx9zh11	303	22	more	more	RBR
cord-328460-thx9zh11	303	23	accurate	accurate	JJ
cord-328460-thx9zh11	303	24	quantification	quantification	NN
cord-328460-thx9zh11	303	25	.	.	.
cord-328460-thx9zh11	304	1	In	in	IN
cord-328460-thx9zh11	304	2	this	this	DT
cord-328460-thx9zh11	304	3	review	review	NN
cord-328460-thx9zh11	304	4	,	,	,
cord-328460-thx9zh11	304	5	the	the	DT
cord-328460-thx9zh11	304	6	use	use	NN
cord-328460-thx9zh11	304	7	of	of	IN
cord-328460-thx9zh11	304	8	microfluidic	microfluidic	NN
cord-328460-thx9zh11	304	9	-	-	HYPH
cord-328460-thx9zh11	304	10	based	base	VBN
cord-328460-thx9zh11	304	11	methods	method	NNS
cord-328460-thx9zh11	304	12	for	for	IN
cord-328460-thx9zh11	304	13	isothermal	isothermal	JJ
cord-328460-thx9zh11	304	14	nucleic	nucleic	JJ
cord-328460-thx9zh11	304	15	acid	acid	NN
cord-328460-thx9zh11	304	16	amplification	amplification	NN
cord-328460-thx9zh11	304	17	has	have	VBZ
cord-328460-thx9zh11	304	18	been	be	VBN
cord-328460-thx9zh11	304	19	described	describe	VBN
cord-328460-thx9zh11	304	20	.	.	.
cord-328460-thx9zh11	305	1	In	in	IN
cord-328460-thx9zh11	305	2	particular	particular	JJ
cord-328460-thx9zh11	305	3	,	,	,
cord-328460-thx9zh11	305	4	the	the	DT
cord-328460-thx9zh11	305	5	integration	integration	NN
cord-328460-thx9zh11	305	6	in	in	IN
cord-328460-thx9zh11	305	7	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	305	8	device	device	NN
cord-328460-thx9zh11	305	9	of	of	IN
cord-328460-thx9zh11	305	10	NASBA	NASBA	NNP
cord-328460-thx9zh11	305	11	,	,	,
cord-328460-thx9zh11	305	12	LAMP	LAMP	NNP
cord-328460-thx9zh11	305	13	,	,	,
cord-328460-thx9zh11	305	14	HDA	HDA	NNP
cord-328460-thx9zh11	305	15	,	,	,
cord-328460-thx9zh11	305	16	RCA	RCA	NNP
cord-328460-thx9zh11	305	17	,	,	,
cord-328460-thx9zh11	305	18	MDA	MDA	NNP
cord-328460-thx9zh11	305	19	and	and	CC
cord-328460-thx9zh11	305	20	RPA	RPA	NNP
cord-328460-thx9zh11	305	21	isothermal	isothermal	JJ
cord-328460-thx9zh11	305	22	amplification	amplification	NN
cord-328460-thx9zh11	305	23	methods	method	NNS
cord-328460-thx9zh11	305	24	have	have	VBP
cord-328460-thx9zh11	305	25	been	be	VBN
cord-328460-thx9zh11	305	26	discussed	discuss	VBN
cord-328460-thx9zh11	305	27	.	.	.
cord-328460-thx9zh11	306	1	Isothermal	isothermal	JJ
cord-328460-thx9zh11	306	2	reactions	reaction	NNS
cord-328460-thx9zh11	306	3	allow	allow	VBP
cord-328460-thx9zh11	306	4	nucleic	nucleic	JJ
cord-328460-thx9zh11	306	5	acid	acid	NN
cord-328460-thx9zh11	306	6	amplification	amplification	NN
cord-328460-thx9zh11	306	7	at	at	IN
cord-328460-thx9zh11	306	8	both	both	CC
cord-328460-thx9zh11	306	9	constant	constant	JJ
cord-328460-thx9zh11	306	10	and	and	CC
cord-328460-thx9zh11	306	11	low	low	JJ
cord-328460-thx9zh11	306	12	temperature	temperature	NN
cord-328460-thx9zh11	306	13	.	.	.
cord-328460-thx9zh11	307	1	Although	although	IN
cord-328460-thx9zh11	307	2	PCR	PCR	NNP
cord-328460-thx9zh11	307	3	is	be	VBZ
cord-328460-thx9zh11	307	4	the	the	DT
cord-328460-thx9zh11	307	5	most	most	RBS
cord-328460-thx9zh11	307	6	widespread	widespread	JJ
cord-328460-thx9zh11	307	7	technology	technology	NN
cord-328460-thx9zh11	307	8	for	for	IN
cord-328460-thx9zh11	307	9	DNA	dna	NN
cord-328460-thx9zh11	307	10	amplification	amplification	NN
cord-328460-thx9zh11	307	11	,	,	,
cord-328460-thx9zh11	307	12	the	the	DT
cord-328460-thx9zh11	307	13	need	need	NN
cord-328460-thx9zh11	307	14	for	for	IN
cord-328460-thx9zh11	307	15	an	an	DT
cord-328460-thx9zh11	307	16	electrically	electrically	RB
cord-328460-thx9zh11	307	17	powered	powered	JJ
cord-328460-thx9zh11	307	18	thermal	thermal	JJ
cord-328460-thx9zh11	307	19	cycler	cycler	NN
cord-328460-thx9zh11	307	20	with	with	IN
cord-328460-thx9zh11	307	21	a	a	DT
cord-328460-thx9zh11	307	22	precise	precise	JJ
cord-328460-thx9zh11	307	23	temperature	temperature	NN
cord-328460-thx9zh11	307	24	control	control	NN
cord-328460-thx9zh11	307	25	and	and	CC
cord-328460-thx9zh11	307	26	for	for	IN
cord-328460-thx9zh11	307	27	an	an	DT
cord-328460-thx9zh11	307	28	optimized	optimize	VBN
cord-328460-thx9zh11	307	29	experimental	experimental	JJ
cord-328460-thx9zh11	307	30	setup	setup	NN
cord-328460-thx9zh11	307	31	makes	make	VBZ
cord-328460-thx9zh11	307	32	the	the	DT
cord-328460-thx9zh11	307	33	use	use	NN
cord-328460-thx9zh11	307	34	of	of	IN
cord-328460-thx9zh11	307	35	PCR	PCR	NNP
cord-328460-thx9zh11	307	36	at	at	IN
cord-328460-thx9zh11	307	37	point	point	NN
cord-328460-thx9zh11	307	38	-	-	HYPH
cord-328460-thx9zh11	307	39	of	of	IN
cord-328460-thx9zh11	307	40	-	-	HYPH
cord-328460-thx9zh11	307	41	care	care	NN
cord-328460-thx9zh11	307	42	settings	setting	NNS
cord-328460-thx9zh11	307	43	more	more	RBR
cord-328460-thx9zh11	307	44	complex	complex	JJ
cord-328460-thx9zh11	307	45	and	and	CC
cord-328460-thx9zh11	307	46	increases	increase	VBZ
cord-328460-thx9zh11	307	47	the	the	DT
cord-328460-thx9zh11	307	48	cost	cost	NN
cord-328460-thx9zh11	307	49	of	of	IN
cord-328460-thx9zh11	307	50	PCR	PCR	NNP
cord-328460-thx9zh11	307	51	-	-	HYPH
cord-328460-thx9zh11	307	52	based	base	VBN
cord-328460-thx9zh11	307	53	devices	device	NNS
cord-328460-thx9zh11	307	54	.	.	.
cord-328460-thx9zh11	308	1	Conversely	conversely	RB
cord-328460-thx9zh11	308	2	,	,	,
cord-328460-thx9zh11	308	3	isothermal	isothermal	JJ
cord-328460-thx9zh11	308	4	amplification	amplification	NN
cord-328460-thx9zh11	308	5	processes	process	NNS
cord-328460-thx9zh11	308	6	offer	offer	VBP
cord-328460-thx9zh11	308	7	multiple	multiple	JJ
cord-328460-thx9zh11	308	8	advantages	advantage	NNS
cord-328460-thx9zh11	308	9	if	if	IN
cord-328460-thx9zh11	308	10	compared	compare	VBN
cord-328460-thx9zh11	308	11	with	with	IN
cord-328460-thx9zh11	308	12	thermal	thermal	JJ
cord-328460-thx9zh11	308	13	cycling	cycling	NN
cord-328460-thx9zh11	308	14	requiring	require	VBG
cord-328460-thx9zh11	308	15	methods	method	NNS
cord-328460-thx9zh11	308	16	,	,	,
cord-328460-thx9zh11	308	17	such	such	JJ
cord-328460-thx9zh11	308	18	as	as	IN
cord-328460-thx9zh11	308	19	reduced	reduced	JJ
cord-328460-thx9zh11	308	20	needs	need	NNS
cord-328460-thx9zh11	308	21	for	for	IN
cord-328460-thx9zh11	308	22	instrumental	instrumental	JJ
cord-328460-thx9zh11	308	23	complexity	complexity	NN
cord-328460-thx9zh11	308	24	,	,	,
cord-328460-thx9zh11	308	25	thermal	thermal	JJ
cord-328460-thx9zh11	308	26	control	control	NN
cord-328460-thx9zh11	308	27	and	and	CC
cord-328460-thx9zh11	308	28	power	power	NN
cord-328460-thx9zh11	308	29	consumption	consumption	NN
cord-328460-thx9zh11	308	30	,	,	,
cord-328460-thx9zh11	308	31	thus	thus	RB
cord-328460-thx9zh11	308	32	rendering	render	VBG
cord-328460-thx9zh11	308	33	their	-PRON-	PRP$
cord-328460-thx9zh11	308	34	integration	integration	NN
cord-328460-thx9zh11	308	35	in	in	IN
cord-328460-thx9zh11	308	36	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	308	37	platforms	platform	NNS
cord-328460-thx9zh11	308	38	significantly	significantly	RB
cord-328460-thx9zh11	308	39	easier	easy	JJR
cord-328460-thx9zh11	308	40	.	.	.
cord-328460-thx9zh11	309	1	In	in	IN
cord-328460-thx9zh11	309	2	addition	addition	NN
cord-328460-thx9zh11	309	3	,	,	,
cord-328460-thx9zh11	309	4	the	the	DT
cord-328460-thx9zh11	309	5	use	use	NN
cord-328460-thx9zh11	309	6	of	of	IN
cord-328460-thx9zh11	309	7	isothermal	isothermal	JJ
cord-328460-thx9zh11	309	8	amplification	amplification	NN
cord-328460-thx9zh11	309	9	techniques	technique	NNS
cord-328460-thx9zh11	309	10	coupled	couple	VBN
cord-328460-thx9zh11	309	11	with	with	IN
cord-328460-thx9zh11	309	12	miniaturized	miniaturized	JJ
cord-328460-thx9zh11	309	13	devices	device	NNS
cord-328460-thx9zh11	309	14	for	for	IN
cord-328460-thx9zh11	309	15	analysis	analysis	NN
cord-328460-thx9zh11	309	16	ensure	ensure	VBP
cord-328460-thx9zh11	309	17	a	a	DT
cord-328460-thx9zh11	309	18	higher	high	JJR
cord-328460-thx9zh11	309	19	percentage	percentage	NN
cord-328460-thx9zh11	309	20	of	of	IN
cord-328460-thx9zh11	309	21	specific	specific	JJ
cord-328460-thx9zh11	309	22	amplification	amplification	NN
cord-328460-thx9zh11	309	23	product	product	NN
cord-328460-thx9zh11	309	24	obtained	obtain	VBN
cord-328460-thx9zh11	309	25	from	from	IN
cord-328460-thx9zh11	309	26	the	the	DT
cord-328460-thx9zh11	309	27	targeted	target	VBN
cord-328460-thx9zh11	309	28	DNA	dna	NN
cord-328460-thx9zh11	309	29	template	template	NN
cord-328460-thx9zh11	309	30	and	and	CC
cord-328460-thx9zh11	309	31	reduced	reduced	JJ
cord-328460-thx9zh11	309	32	amplification	amplification	NN
cord-328460-thx9zh11	309	33	bias	bias	NN
cord-328460-thx9zh11	309	34	,	,	,
cord-328460-thx9zh11	309	35	enabling	enable	VBG
cord-328460-thx9zh11	309	36	also	also	RB
cord-328460-thx9zh11	309	37	accurate	accurate	JJ
cord-328460-thx9zh11	309	38	and	and	CC
cord-328460-thx9zh11	309	39	high	high	JJ
cord-328460-thx9zh11	309	40	fidelity	fidelity	NN
cord-328460-thx9zh11	309	41	single	single	JJ
cord-328460-thx9zh11	309	42	-	-	HYPH
cord-328460-thx9zh11	309	43	cell	cell	NN
cord-328460-thx9zh11	309	44	genome	genome	NN
cord-328460-thx9zh11	309	45	amplification	amplification	NN
cord-328460-thx9zh11	309	46	in	in	IN
cord-328460-thx9zh11	309	47	nanoliter	nanoliter	JJ
cord-328460-thx9zh11	309	48	volumes	volume	NNS
cord-328460-thx9zh11	309	49	.	.	.
cord-328460-thx9zh11	309	50	 	 	_SP
cord-328460-thx9zh11	310	1	Direct	direct	JJ
cord-328460-thx9zh11	310	2	detection	detection	NN
cord-328460-thx9zh11	310	3	of	of	IN
cord-328460-thx9zh11	310	4	point	point	NN
cord-328460-thx9zh11	310	5	mutations	mutation	NNS
cord-328460-thx9zh11	310	6	in	in	IN
cord-328460-thx9zh11	310	7	nonamplified	nonamplified	JJ
cord-328460-thx9zh11	310	8	human	human	JJ
cord-328460-thx9zh11	310	9	genomic	genomic	JJ
cord-328460-thx9zh11	310	10	DNA	dna	NN
cord-328460-thx9zh11	310	11	DNA	dna	NN
cord-328460-thx9zh11	310	12	analyses	analysis	NNS
cord-328460-thx9zh11	310	13	in	in	IN
cord-328460-thx9zh11	310	14	food	food	NN
cord-328460-thx9zh11	310	15	safety	safety	NN
cord-328460-thx9zh11	310	16	and	and	CC
cord-328460-thx9zh11	310	17	quality	quality	NN
cord-328460-thx9zh11	310	18	:	:	:
cord-328460-thx9zh11	310	19	Current	current	JJ
cord-328460-thx9zh11	310	20	status	status	NN
cord-328460-thx9zh11	310	21	and	and	CC
cord-328460-thx9zh11	310	22	expectations	expectation	NNS
cord-328460-thx9zh11	310	23	Ultrasensitive	ultrasensitive	JJ
cord-328460-thx9zh11	310	24	detection	detection	NN
cord-328460-thx9zh11	310	25	of	of	IN
cord-328460-thx9zh11	310	26	non	non	JJ
cord-328460-thx9zh11	310	27	-	-	JJ
cord-328460-thx9zh11	310	28	amplified	amplified	JJ
cord-328460-thx9zh11	310	29	genomic	genomic	JJ
cord-328460-thx9zh11	310	30	DNA	dna	NN
cord-328460-thx9zh11	310	31	by	by	IN
cord-328460-thx9zh11	310	32	nanoparticle	nanoparticle	NN
cord-328460-thx9zh11	310	33	-	-	HYPH
cord-328460-thx9zh11	310	34	enhanced	enhance	VBN
cord-328460-thx9zh11	310	35	Surface	Surface	NNP
cord-328460-thx9zh11	310	36	-	-	HYPH
cord-328460-thx9zh11	310	37	Plasmon	Plasmon	NNP
cord-328460-thx9zh11	310	38	Resonance	Resonance	NNP
cord-328460-thx9zh11	310	39	Imaging	Imaging	NNP
cord-328460-thx9zh11	310	40	Reproducible	reproducible	JJ
cord-328460-thx9zh11	310	41	and	and	CC
cord-328460-thx9zh11	310	42	reliable	reliable	JJ
cord-328460-thx9zh11	310	43	microarray	microarray	NN
cord-328460-thx9zh11	310	44	results	result	NNS
cord-328460-thx9zh11	310	45	through	through	IN
cord-328460-thx9zh11	310	46	quality	quality	NN
cord-328460-thx9zh11	310	47	control	control	NN
cord-328460-thx9zh11	310	48	:	:	:
cord-328460-thx9zh11	310	49	Good	good	JJ
cord-328460-thx9zh11	310	50	laboratory	laboratory	NN
cord-328460-thx9zh11	310	51	proficiency	proficiency	NN
cord-328460-thx9zh11	310	52	and	and	CC
cord-328460-thx9zh11	310	53	appropriate	appropriate	JJ
cord-328460-thx9zh11	310	54	data	data	NN
cord-328460-thx9zh11	310	55	analysis	analysis	NN
cord-328460-thx9zh11	310	56	practices	practice	NNS
cord-328460-thx9zh11	310	57	are	be	VBP
cord-328460-thx9zh11	310	58	essential	essential	JJ
cord-328460-thx9zh11	310	59	Surface	Surface	NNP
cord-328460-thx9zh11	310	60	Plasmon	Plasmon	NNP
cord-328460-thx9zh11	310	61	Resonance	Resonance	NNP
cord-328460-thx9zh11	310	62	imaging	imaging	NN
cord-328460-thx9zh11	310	63	:	:	:
cord-328460-thx9zh11	311	1	What	what	WP
cord-328460-thx9zh11	311	2	's	be	VBZ
cord-328460-thx9zh11	311	3	next	next	JJ
cord-328460-thx9zh11	311	4	?	?	.
cord-328460-thx9zh11	312	1	Functionalized	functionalized	JJ
cord-328460-thx9zh11	312	2	gold	gold	NN
cord-328460-thx9zh11	312	3	nanoparticles	nanoparticle	NNS
cord-328460-thx9zh11	312	4	for	for	IN
cord-328460-thx9zh11	312	5	the	the	DT
cord-328460-thx9zh11	312	6	ultrasensitive	ultrasensitive	JJ
cord-328460-thx9zh11	312	7	DNA	dna	NN
cord-328460-thx9zh11	312	8	detection	detection	NN
cord-328460-thx9zh11	312	9	Miniaturized	miniaturize	VBD
cord-328460-thx9zh11	312	10	isothermal	isothermal	JJ
cord-328460-thx9zh11	312	11	nucleic	nucleic	JJ
cord-328460-thx9zh11	312	12	acid	acid	NN
cord-328460-thx9zh11	312	13	amplification	amplification	NN
cord-328460-thx9zh11	312	14	,	,	,
cord-328460-thx9zh11	312	15	a	a	DT
cord-328460-thx9zh11	312	16	review	review	NN
cord-328460-thx9zh11	312	17	Agarose	Agarose	NNP
cord-328460-thx9zh11	312	18	droplet	droplet	NN
cord-328460-thx9zh11	312	19	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	312	20	for	for	IN
cord-328460-thx9zh11	312	21	highly	highly	RB
cord-328460-thx9zh11	312	22	parallel	parallel	JJ
cord-328460-thx9zh11	312	23	and	and	CC
cord-328460-thx9zh11	312	24	efficient	efficient	JJ
cord-328460-thx9zh11	312	25	single	single	JJ
cord-328460-thx9zh11	312	26	molecule	molecule	NN
cord-328460-thx9zh11	312	27	emulsion	emulsion	NN
cord-328460-thx9zh11	312	28	PCR	PCR	NNP
cord-328460-thx9zh11	312	29	PCR	PCR	NNP
cord-328460-thx9zh11	312	30	amplification	amplification	NN
cord-328460-thx9zh11	312	31	from	from	IN
cord-328460-thx9zh11	312	32	single	single	JJ
cord-328460-thx9zh11	312	33	DNA	dna	NN
cord-328460-thx9zh11	312	34	molecules	molecule	NNS
cord-328460-thx9zh11	312	35	on	on	IN
cord-328460-thx9zh11	312	36	magnetic	magnetic	JJ
cord-328460-thx9zh11	312	37	beads	bead	NNS
cord-328460-thx9zh11	312	38	in	in	IN
cord-328460-thx9zh11	312	39	emulsion	emulsion	NN
cord-328460-thx9zh11	312	40	:	:	:
cord-328460-thx9zh11	312	41	application	application	NN
cord-328460-thx9zh11	312	42	for	for	IN
cord-328460-thx9zh11	312	43	high	high	JJ
cord-328460-thx9zh11	312	44	-	-	HYPH
cord-328460-thx9zh11	312	45	throughput	throughput	NN
cord-328460-thx9zh11	312	46	screening	screening	NN
cord-328460-thx9zh11	312	47	of	of	IN
cord-328460-thx9zh11	312	48	transcription	transcription	NN
cord-328460-thx9zh11	312	49	factor	factor	NN
cord-328460-thx9zh11	312	50	targets	target	VBZ
cord-328460-thx9zh11	312	51	Isothermal	Isothermal	NNP
cord-328460-thx9zh11	312	52	DNA	dna	NN
cord-328460-thx9zh11	312	53	amplification	amplification	NN
cord-328460-thx9zh11	312	54	in	in	IN
cord-328460-thx9zh11	312	55	bioanalysis	bioanalysis	NN
cord-328460-thx9zh11	312	56	:	:	:
cord-328460-thx9zh11	312	57	Strategies	strategy	NNS
cord-328460-thx9zh11	312	58	and	and	CC
cord-328460-thx9zh11	312	59	applications	application	NNS
cord-328460-thx9zh11	313	1	The	the	DT
cord-328460-thx9zh11	313	2	origins	origin	NNS
cord-328460-thx9zh11	313	3	and	and	CC
cord-328460-thx9zh11	313	4	future	future	NN
cord-328460-thx9zh11	313	5	of	of	IN
cord-328460-thx9zh11	313	6	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	313	7	Physics	Physics	NNP
cord-328460-thx9zh11	313	8	and	and	CC
cord-328460-thx9zh11	313	9	applications	application	NNS
cord-328460-thx9zh11	313	10	of	of	IN
cord-328460-thx9zh11	313	11	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	313	12	in	in	IN
cord-328460-thx9zh11	313	13	biology	biology	NN
cord-328460-thx9zh11	313	14	Engineering	engineering	NN
cord-328460-thx9zh11	313	15	flows	flow	NNS
cord-328460-thx9zh11	313	16	in	in	IN
cord-328460-thx9zh11	313	17	small	small	JJ
cord-328460-thx9zh11	313	18	devices	device	NNS
cord-328460-thx9zh11	313	19	:	:	:
cord-328460-thx9zh11	314	1	Microfluidics	microfluidic	NNS
cord-328460-thx9zh11	314	2	toward	toward	IN
cord-328460-thx9zh11	314	3	a	a	DT
cord-328460-thx9zh11	314	4	lab	lab	NN
cord-328460-thx9zh11	314	5	-	-	HYPH
cord-328460-thx9zh11	314	6	on	on	IN
cord-328460-thx9zh11	314	7	-	-	HYPH
cord-328460-thx9zh11	314	8	a	a	DT
cord-328460-thx9zh11	314	9	-	-	HYPH
cord-328460-thx9zh11	314	10	chip	chip	NN
cord-328460-thx9zh11	314	11	Microfluidics	microfluidic	NNS
cord-328460-thx9zh11	314	12	:	:	:
cord-328460-thx9zh11	315	1	Fluid	fluid	JJ
cord-328460-thx9zh11	315	2	physics	physics	NN
cord-328460-thx9zh11	315	3	at	at	IN
cord-328460-thx9zh11	315	4	the	the	DT
cord-328460-thx9zh11	315	5	nanoliter	nanoliter	NNP
cord-328460-thx9zh11	315	6	scale	scale	NN
cord-328460-thx9zh11	316	1	Studying	study	VBG
cord-328460-thx9zh11	316	2	enzymatic	enzymatic	JJ
cord-328460-thx9zh11	316	3	bioreactions	bioreaction	NNS
cord-328460-thx9zh11	316	4	in	in	IN
cord-328460-thx9zh11	316	5	a	a	DT
cord-328460-thx9zh11	316	6	millisecond	millisecond	NN
cord-328460-thx9zh11	316	7	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	316	8	flow	flow	NN
cord-328460-thx9zh11	316	9	mixer	mixer	NN
cord-328460-thx9zh11	317	1	A	a	DT
cord-328460-thx9zh11	317	2	highly	highly	RB
cord-328460-thx9zh11	317	3	uniform	uniform	JJ
cord-328460-thx9zh11	317	4	lamination	lamination	NN
cord-328460-thx9zh11	317	5	micromixer	micromixer	NN
cord-328460-thx9zh11	317	6	with	with	IN
cord-328460-thx9zh11	317	7	wedge	wedge	NN
cord-328460-thx9zh11	317	8	shaped	shape	VBN
cord-328460-thx9zh11	317	9	inlet	inlet	NN
cord-328460-thx9zh11	317	10	channels	channel	NNS
cord-328460-thx9zh11	317	11	for	for	IN
cord-328460-thx9zh11	317	12	time	time	NN
cord-328460-thx9zh11	317	13	resolved	resolve	VBD
cord-328460-thx9zh11	317	14	infrared	infrared	JJ
cord-328460-thx9zh11	317	15	spectroscopy	spectroscopy	NN
cord-328460-thx9zh11	317	16	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	317	17	mixing	mix	VBG
cord-328460-thx9zh11	317	18	:	:	:
cord-328460-thx9zh11	317	19	A	a	DT
cord-328460-thx9zh11	317	20	review	review	NN
cord-328460-thx9zh11	317	21	Flow	flow	NN
cord-328460-thx9zh11	317	22	-	-	HYPH
cord-328460-thx9zh11	317	23	based	base	VBN
cord-328460-thx9zh11	317	24	systems	system	NNS
cord-328460-thx9zh11	317	25	for	for	IN
cord-328460-thx9zh11	317	26	rapid	rapid	JJ
cord-328460-thx9zh11	317	27	and	and	CC
cord-328460-thx9zh11	317	28	high	high	JJ
cord-328460-thx9zh11	317	29	-	-	HYPH
cord-328460-thx9zh11	317	30	precision	precision	NN
cord-328460-thx9zh11	317	31	enzyme	enzyme	NN
cord-328460-thx9zh11	317	32	kinetics	kinetics	NN
cord-328460-thx9zh11	317	33	studies	study	NNS
cord-328460-thx9zh11	317	34	cost	cost	NN
cord-328460-thx9zh11	317	35	-	-	HYPH
cord-328460-thx9zh11	317	36	efficient	efficient	JJ
cord-328460-thx9zh11	317	37	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	317	38	-	-	HYPH
cord-328460-thx9zh11	317	39	based	base	VBN
cord-328460-thx9zh11	317	40	stopped	stop	VBN
cord-328460-thx9zh11	317	41	-	-	HYPH
cord-328460-thx9zh11	317	42	flow	flow	NN
cord-328460-thx9zh11	317	43	device	device	NN
cord-328460-thx9zh11	317	44	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	317	45	DNA	DNA	NNP
cord-328460-thx9zh11	317	46	amplification-	amplification-	NN
cord-328460-thx9zh11	318	1	A	a	DT
cord-328460-thx9zh11	318	2	review	review	NN
cord-328460-thx9zh11	318	3	Rapid	Rapid	NNP
cord-328460-thx9zh11	318	4	PCR	PCR	NNP
cord-328460-thx9zh11	318	5	amplification	amplification	NN
cord-328460-thx9zh11	318	6	using	use	VBG
cord-328460-thx9zh11	318	7	a	a	DT
cord-328460-thx9zh11	318	8	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	318	9	device	device	NN
cord-328460-thx9zh11	318	10	with	with	IN
cord-328460-thx9zh11	318	11	integrated	integrate	VBN
cord-328460-thx9zh11	318	12	microwave	microwave	NN
cord-328460-thx9zh11	318	13	heating	heating	NN
cord-328460-thx9zh11	318	14	and	and	CC
cord-328460-thx9zh11	318	15	air	air	NN
cord-328460-thx9zh11	318	16	impingement	impingement	NN
cord-328460-thx9zh11	318	17	cooling	cool	VBG
cord-328460-thx9zh11	318	18	Integrated	Integrated	NNP
cord-328460-thx9zh11	318	19	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	318	20	reverse	reverse	JJ
cord-328460-thx9zh11	318	21	transcription	transcription	NN
cord-328460-thx9zh11	318	22	-	-	HYPH
cord-328460-thx9zh11	318	23	polymerase	polymerase	NN
cord-328460-thx9zh11	318	24	chain	chain	NN
cord-328460-thx9zh11	318	25	reaction	reaction	NN
cord-328460-thx9zh11	318	26	for	for	IN
cord-328460-thx9zh11	318	27	rapid	rapid	JJ
cord-328460-thx9zh11	318	28	detection	detection	NN
cord-328460-thx9zh11	318	29	of	of	IN
cord-328460-thx9zh11	318	30	food	food	NN
cord-328460-thx9zh11	318	31	-	-	HYPH
cord-328460-thx9zh11	318	32	or	or	CC
cord-328460-thx9zh11	318	33	waterborne	waterborne	JJ
cord-328460-thx9zh11	318	34	pathogenic	pathogenic	JJ
cord-328460-thx9zh11	318	35	rotavirus	rotavirus	NN
cord-328460-thx9zh11	318	36	Massively	massively	RB
cord-328460-thx9zh11	318	37	parallel	parallel	JJ
cord-328460-thx9zh11	318	38	single	single	JJ
cord-328460-thx9zh11	318	39	-	-	HYPH
cord-328460-thx9zh11	318	40	molecule	molecule	NN
cord-328460-thx9zh11	318	41	and	and	CC
cord-328460-thx9zh11	318	42	single	single	JJ
cord-328460-thx9zh11	318	43	-	-	HYPH
cord-328460-thx9zh11	318	44	cell	cell	NN
cord-328460-thx9zh11	318	45	emulsion	emulsion	NN
cord-328460-thx9zh11	318	46	reverse	reverse	NN
cord-328460-thx9zh11	318	47	transcription	transcription	NN
cord-328460-thx9zh11	318	48	polymerase	polymerase	NN
cord-328460-thx9zh11	318	49	chain	chain	NN
cord-328460-thx9zh11	318	50	reaction	reaction	NN
cord-328460-thx9zh11	318	51	using	use	VBG
cord-328460-thx9zh11	318	52	Agarose	Agarose	NNP
cord-328460-thx9zh11	318	53	droplet	droplet	NN
cord-328460-thx9zh11	318	54	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	318	55	Adsorption	Adsorption	NNP
cord-328460-thx9zh11	318	56	kinetics	kinetic	NNS
cord-328460-thx9zh11	318	57	of	of	IN
cord-328460-thx9zh11	318	58	proteins	protein	NNS
cord-328460-thx9zh11	318	59	in	in	IN
cord-328460-thx9zh11	318	60	plastic	plastic	NN
cord-328460-thx9zh11	318	61	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	318	62	channels	channel	NNS
cord-328460-thx9zh11	318	63	:	:	:
cord-328460-thx9zh11	318	64	Real	real	JJ
cord-328460-thx9zh11	318	65	-	-	HYPH
cord-328460-thx9zh11	318	66	time	time	NN
cord-328460-thx9zh11	318	67	monitoring	monitoring	NN
cord-328460-thx9zh11	318	68	of	of	IN
cord-328460-thx9zh11	318	69	lysozyme	lysozyme	NNS
cord-328460-thx9zh11	318	70	adsorption	adsorption	NN
cord-328460-thx9zh11	318	71	by	by	IN
cord-328460-thx9zh11	318	72	pulsed	pulse	VBN
cord-328460-thx9zh11	318	73	streaming	streaming	NN
cord-328460-thx9zh11	318	74	potentials	potential	NNS
cord-328460-thx9zh11	318	75	PCR	PCR	NNP
cord-328460-thx9zh11	318	76	biocompatibility	biocompatibility	NN
cord-328460-thx9zh11	318	77	of	of	IN
cord-328460-thx9zh11	318	78	lab	lab	NN
cord-328460-thx9zh11	318	79	-	-	HYPH
cord-328460-thx9zh11	318	80	on	on	IN
cord-328460-thx9zh11	318	81	-	-	HYPH
cord-328460-thx9zh11	318	82	a	a	DT
cord-328460-thx9zh11	318	83	-	-	HYPH
cord-328460-thx9zh11	318	84	chip	chip	NN
cord-328460-thx9zh11	318	85	and	and	CC
cord-328460-thx9zh11	318	86	MEMS	MEMS	NNP
cord-328460-thx9zh11	318	87	materials	material	NNS
cord-328460-thx9zh11	318	88	Biochemical	biochemical	JJ
cord-328460-thx9zh11	318	89	analysis	analysis	NN
cord-328460-thx9zh11	318	90	and	and	CC
cord-328460-thx9zh11	318	91	optimization	optimization	NN
cord-328460-thx9zh11	318	92	of	of	IN
cord-328460-thx9zh11	318	93	inhibition	inhibition	NN
cord-328460-thx9zh11	318	94	and	and	CC
cord-328460-thx9zh11	318	95	adsorption	adsorption	NN
cord-328460-thx9zh11	318	96	phenomena	phenomenon	NNS
cord-328460-thx9zh11	318	97	in	in	IN
cord-328460-thx9zh11	318	98	glass	glass	NN
cord-328460-thx9zh11	318	99	-	-	HYPH
cord-328460-thx9zh11	318	100	silicon	silicon	NN
cord-328460-thx9zh11	318	101	PCR	PCR	NNP
cord-328460-thx9zh11	318	102	-	-	HYPH
cord-328460-thx9zh11	318	103	chips	chip	NNS
cord-328460-thx9zh11	318	104	Development	Development	NNP
cord-328460-thx9zh11	318	105	of	of	IN
cord-328460-thx9zh11	318	106	a	a	DT
cord-328460-thx9zh11	318	107	CMOS	CMOS	NNP
cord-328460-thx9zh11	318	108	-	-	HYPH
cord-328460-thx9zh11	318	109	compatible	compatible	JJ
cord-328460-thx9zh11	318	110	PCR	PCR	NNP
cord-328460-thx9zh11	318	111	chip	chip	NN
cord-328460-thx9zh11	318	112	:	:	:
cord-328460-thx9zh11	319	1	Comparison	comparison	NN
cord-328460-thx9zh11	319	2	of	of	IN
cord-328460-thx9zh11	319	3	design	design	NN
cord-328460-thx9zh11	319	4	and	and	CC
cord-328460-thx9zh11	319	5	system	system	NN
cord-328460-thx9zh11	319	6	strategies	strategy	NNS
cord-328460-thx9zh11	319	7	Miniaturized	miniaturize	VBD
cord-328460-thx9zh11	319	8	PCR	PCR	NNP
cord-328460-thx9zh11	319	9	chips	chip	NNS
cord-328460-thx9zh11	319	10	for	for	IN
cord-328460-thx9zh11	319	11	nucleic	nucleic	JJ
cord-328460-thx9zh11	319	12	acid	acid	NN
cord-328460-thx9zh11	319	13	amplification	amplification	NN
cord-328460-thx9zh11	319	14	and	and	CC
cord-328460-thx9zh11	319	15	analysis	analysis	NN
cord-328460-thx9zh11	319	16	:	:	:
cord-328460-thx9zh11	319	17	Latest	late	JJS
cord-328460-thx9zh11	319	18	advances	advance	NNS
cord-328460-thx9zh11	319	19	and	and	CC
cord-328460-thx9zh11	319	20	future	future	JJ
cord-328460-thx9zh11	319	21	trends	trend	NNS
cord-328460-thx9zh11	319	22	Investigations	investigation	NNS
cord-328460-thx9zh11	319	23	on	on	IN
cord-328460-thx9zh11	319	24	the	the	DT
cord-328460-thx9zh11	319	25	compatibility	compatibility	NN
cord-328460-thx9zh11	319	26	of	of	IN
cord-328460-thx9zh11	319	27	chemically	chemically	RB
cord-328460-thx9zh11	319	28	oxidized	oxidize	VBN
cord-328460-thx9zh11	319	29	silicon	silicon	NN
cord-328460-thx9zh11	319	30	(	(	-LRB-
cord-328460-thx9zh11	319	31	SiOx)-surfaces	siox)-surface	NNS
cord-328460-thx9zh11	319	32	for	for	IN
cord-328460-thx9zh11	319	33	applications	application	NNS
cord-328460-thx9zh11	319	34	towards	towards	IN
cord-328460-thx9zh11	319	35	chip	chip	NN
cord-328460-thx9zh11	319	36	-	-	HYPH
cord-328460-thx9zh11	319	37	based	base	VBN
cord-328460-thx9zh11	319	38	polymerase	polymerase	NN
cord-328460-thx9zh11	319	39	chain	chain	NN
cord-328460-thx9zh11	319	40	reaction	reaction	NN
cord-328460-thx9zh11	319	41	Surface	surface	NN
cord-328460-thx9zh11	319	42	effects	effect	NNS
cord-328460-thx9zh11	319	43	on	on	IN
cord-328460-thx9zh11	319	44	PCR	PCR	NNP
cord-328460-thx9zh11	319	45	reactions	reaction	NNS
cord-328460-thx9zh11	319	46	in	in	IN
cord-328460-thx9zh11	319	47	multichip	multichip	NN
cord-328460-thx9zh11	319	48	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	319	49	platforms	platform	NNS
cord-328460-thx9zh11	319	50	Peptide	peptide	NN
cord-328460-thx9zh11	319	51	nucleic	nucleic	JJ
cord-328460-thx9zh11	319	52	acid	acid	NN
cord-328460-thx9zh11	319	53	molecular	molecular	JJ
cord-328460-thx9zh11	319	54	beacons	beacon	NNS
cord-328460-thx9zh11	319	55	for	for	IN
cord-328460-thx9zh11	319	56	the	the	DT
cord-328460-thx9zh11	319	57	detection	detection	NN
cord-328460-thx9zh11	319	58	of	of	IN
cord-328460-thx9zh11	319	59	PCR	PCR	NNP
cord-328460-thx9zh11	319	60	amplicons	amplicon	NNS
cord-328460-thx9zh11	319	61	in	in	IN
cord-328460-thx9zh11	319	62	droplet	droplet	NN
cord-328460-thx9zh11	319	63	-	-	HYPH
cord-328460-thx9zh11	319	64	based	base	VBN
cord-328460-thx9zh11	319	65	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	319	66	devices	device	NNS
cord-328460-thx9zh11	319	67	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	319	68	methods	method	NNS
cord-328460-thx9zh11	319	69	for	for	IN
cord-328460-thx9zh11	319	70	generating	generate	VBG
cord-328460-thx9zh11	319	71	continuous	continuous	JJ
cord-328460-thx9zh11	319	72	droplet	droplet	NN
cord-328460-thx9zh11	319	73	streams	stream	NNS
cord-328460-thx9zh11	320	1	Miniaturising	miniaturise	VBG
cord-328460-thx9zh11	320	2	the	the	DT
cord-328460-thx9zh11	320	3	laboratory	laboratory	NN
cord-328460-thx9zh11	320	4	in	in	IN
cord-328460-thx9zh11	320	5	emulsion	emulsion	NN
cord-328460-thx9zh11	320	6	droplets	droplet	NNS
cord-328460-thx9zh11	320	7	Digital	Digital	NNP
cord-328460-thx9zh11	320	8	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	321	1	:	:	:
cord-328460-thx9zh11	321	2	Is	be	VBZ
cord-328460-thx9zh11	321	3	a	a	DT
cord-328460-thx9zh11	321	4	true	true	JJ
cord-328460-thx9zh11	321	5	lab	lab	NN
cord-328460-thx9zh11	321	6	-	-	HYPH
cord-328460-thx9zh11	321	7	on	on	IN
cord-328460-thx9zh11	321	8	-	-	HYPH
cord-328460-thx9zh11	321	9	a	a	DT
cord-328460-thx9zh11	321	10	-	-	HYPH
cord-328460-thx9zh11	321	11	chip	chip	NN
cord-328460-thx9zh11	321	12	possible	possible	JJ
cord-328460-thx9zh11	321	13	?	?	.
cord-328460-thx9zh11	322	1	Microfluid	Microfluid	NNP
cord-328460-thx9zh11	322	2	.	.	.
cord-328460-thx9zh11	323	1	Nanofluid	Nanofluid	NNP
cord-328460-thx9zh11	323	2	High	high	JJ
cord-328460-thx9zh11	323	3	-	-	HYPH
cord-328460-thx9zh11	323	4	performance	performance	NN
cord-328460-thx9zh11	323	5	single	single	JJ
cord-328460-thx9zh11	323	6	cell	cell	NN
cord-328460-thx9zh11	323	7	genetic	genetic	JJ
cord-328460-thx9zh11	323	8	analysis	analysis	NN
cord-328460-thx9zh11	323	9	using	use	VBG
cord-328460-thx9zh11	323	10	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	323	11	emulsion	emulsion	NN
cord-328460-thx9zh11	323	12	generator	generator	NN
cord-328460-thx9zh11	323	13	arrays	array	NNS
cord-328460-thx9zh11	323	14	High	high	JJ
cord-328460-thx9zh11	323	15	-	-	HYPH
cord-328460-thx9zh11	323	16	throughput	throughput	NN
cord-328460-thx9zh11	323	17	quantitative	quantitative	JJ
cord-328460-thx9zh11	323	18	polymerase	polymerase	NN
cord-328460-thx9zh11	323	19	chain	chain	NN
cord-328460-thx9zh11	323	20	reaction	reaction	NN
cord-328460-thx9zh11	323	21	in	in	IN
cord-328460-thx9zh11	323	22	picoliter	picoliter	NN
cord-328460-thx9zh11	323	23	droplets	droplet	NNS
cord-328460-thx9zh11	323	24	Multiplexed	Multiplexed	NNP
cord-328460-thx9zh11	323	25	real	real	JJ
cord-328460-thx9zh11	323	26	-	-	HYPH
cord-328460-thx9zh11	323	27	time	time	NN
cord-328460-thx9zh11	323	28	polymerase	polymerase	NN
cord-328460-thx9zh11	323	29	chain	chain	NN
cord-328460-thx9zh11	323	30	reaction	reaction	NN
cord-328460-thx9zh11	323	31	on	on	IN
cord-328460-thx9zh11	323	32	a	a	DT
cord-328460-thx9zh11	323	33	digital	digital	JJ
cord-328460-thx9zh11	323	34	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	323	35	platform	platform	NN
cord-328460-thx9zh11	323	36	Single	single	JJ
cord-328460-thx9zh11	323	37	-	-	HYPH
cord-328460-thx9zh11	323	38	molecule	molecule	NN
cord-328460-thx9zh11	323	39	emulsion	emulsion	NN
cord-328460-thx9zh11	323	40	PCR	PCR	NNP
cord-328460-thx9zh11	323	41	in	in	IN
cord-328460-thx9zh11	323	42	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	323	43	droplets	droplet	NNS
cord-328460-thx9zh11	323	44	Loop	loop	NN
cord-328460-thx9zh11	323	45	-	-	HYPH
cord-328460-thx9zh11	323	46	mediated	mediate	VBN
cord-328460-thx9zh11	323	47	isothermal	isothermal	JJ
cord-328460-thx9zh11	323	48	amplification	amplification	NN
cord-328460-thx9zh11	323	49	of	of	IN
cord-328460-thx9zh11	323	50	DNA	DNA	NNP
cord-328460-thx9zh11	323	51	Rapid	Rapid	NNP
cord-328460-thx9zh11	323	52	detection	detection	NN
cord-328460-thx9zh11	323	53	of	of	IN
cord-328460-thx9zh11	323	54	HIV-1	HIV-1	NNP
cord-328460-thx9zh11	323	55	by	by	IN
cord-328460-thx9zh11	323	56	reverse	reverse	NN
cord-328460-thx9zh11	323	57	-	-	HYPH
cord-328460-thx9zh11	323	58	transcription	transcription	NN
cord-328460-thx9zh11	323	59	,	,	,
cord-328460-thx9zh11	323	60	loop	loop	NN
cord-328460-thx9zh11	323	61	-	-	HYPH
cord-328460-thx9zh11	323	62	mediated	mediate	VBN
cord-328460-thx9zh11	323	63	isothermal	isothermal	JJ
cord-328460-thx9zh11	323	64	amplification	amplification	NN
cord-328460-thx9zh11	323	65	(	(	-LRB-
cord-328460-thx9zh11	323	66	RT	RT	NNP
cord-328460-thx9zh11	323	67	-	-	HYPH
cord-328460-thx9zh11	323	68	LAMP	LAMP	NNP
cord-328460-thx9zh11	323	69	)	)	-RRB-
cord-328460-thx9zh11	323	70	Development	development	NN
cord-328460-thx9zh11	323	71	and	and	CC
cord-328460-thx9zh11	323	72	evaluation	evaluation	NN
cord-328460-thx9zh11	323	73	of	of	IN
cord-328460-thx9zh11	323	74	a	a	DT
cord-328460-thx9zh11	323	75	novel	novel	JJ
cord-328460-thx9zh11	323	76	loop	loop	NN
cord-328460-thx9zh11	323	77	-	-	HYPH
cord-328460-thx9zh11	323	78	mediated	mediate	VBN
cord-328460-thx9zh11	323	79	isothermal	isothermal	JJ
cord-328460-thx9zh11	323	80	amplification	amplification	NN
cord-328460-thx9zh11	323	81	method	method	NN
cord-328460-thx9zh11	323	82	for	for	IN
cord-328460-thx9zh11	323	83	rapid	rapid	JJ
cord-328460-thx9zh11	323	84	detection	detection	NN
cord-328460-thx9zh11	323	85	of	of	IN
cord-328460-thx9zh11	323	86	severe	severe	JJ
cord-328460-thx9zh11	323	87	acute	acute	JJ
cord-328460-thx9zh11	323	88	respiratory	respiratory	JJ
cord-328460-thx9zh11	323	89	syndrome	syndrome	NN
cord-328460-thx9zh11	323	90	coronavirus	coronavirus	NN
cord-328460-thx9zh11	323	91	Application	Application	NNP
cord-328460-thx9zh11	323	92	of	of	IN
cord-328460-thx9zh11	323	93	loop	loop	NN
cord-328460-thx9zh11	323	94	-	-	HYPH
cord-328460-thx9zh11	323	95	mediated	mediate	VBN
cord-328460-thx9zh11	323	96	isothermal	isothermal	JJ
cord-328460-thx9zh11	323	97	amplifi	amplifi	NNP
cord-328460-thx9zh11	323	98	cation	cation	NN
cord-328460-thx9zh11	323	99	technique	technique	NN
cord-328460-thx9zh11	323	100	to	to	IN
cord-328460-thx9zh11	323	101	rapid	rapid	JJ
cord-328460-thx9zh11	323	102	and	and	CC
cord-328460-thx9zh11	323	103	direct	direct	JJ
cord-328460-thx9zh11	323	104	detection	detection	NN
cord-328460-thx9zh11	323	105	of	of	IN
cord-328460-thx9zh11	323	106	methicillin	methicillin	NN
cord-328460-thx9zh11	323	107	-	-	HYPH
cord-328460-thx9zh11	323	108	resistant	resistant	JJ
cord-328460-thx9zh11	323	109	Staphylococcus	Staphylococcus	NNP
cord-328460-thx9zh11	323	110	aureus	aureus	NN
cord-328460-thx9zh11	323	111	(	(	-LRB-
cord-328460-thx9zh11	323	112	MRSA	MRSA	NNP
cord-328460-thx9zh11	323	113	)	)	-RRB-
cord-328460-thx9zh11	323	114	in	in	IN
cord-328460-thx9zh11	323	115	blood	blood	NN
cord-328460-thx9zh11	323	116	cultures	culture	NNS
cord-328460-thx9zh11	323	117	Enzymic	Enzymic	NNP
cord-328460-thx9zh11	323	118	unwinding	unwinding	NN
cord-328460-thx9zh11	323	119	of	of	IN
cord-328460-thx9zh11	323	120	DNA	dna	NN
cord-328460-thx9zh11	323	121	.	.	.
cord-328460-thx9zh11	324	1	1	1	LS
cord-328460-thx9zh11	324	2	:	:	:
cord-328460-thx9zh11	324	3	Purification	purification	NN
cord-328460-thx9zh11	324	4	and	and	CC
cord-328460-thx9zh11	324	5	characterization	characterization	NN
cord-328460-thx9zh11	324	6	of	of	IN
cord-328460-thx9zh11	324	7	a	a	DT
cord-328460-thx9zh11	324	8	DNA	dna	NN
cord-328460-thx9zh11	324	9	-	-	HYPH
cord-328460-thx9zh11	324	10	dependent	dependent	JJ
cord-328460-thx9zh11	324	11	ATPase	ATPase	NNP
cord-328460-thx9zh11	324	12	from	from	IN
cord-328460-thx9zh11	324	13	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	324	14	coli	coli	NNS
cord-328460-thx9zh11	324	15	Enzymic	Enzymic	NNP
cord-328460-thx9zh11	324	16	unwinding	unwinding	NN
cord-328460-thx9zh11	324	17	of	of	IN
cord-328460-thx9zh11	324	18	DNA	dna	NN
cord-328460-thx9zh11	324	19	.	.	.
cord-328460-thx9zh11	325	1	2	2	LS
cord-328460-thx9zh11	325	2	.	.	.
cord-328460-thx9zh11	326	1	Chain	chain	NN
cord-328460-thx9zh11	326	2	separation	separation	NN
cord-328460-thx9zh11	326	3	by	by	IN
cord-328460-thx9zh11	326	4	an	an	DT
cord-328460-thx9zh11	326	5	ATP	ATP	NNP
cord-328460-thx9zh11	326	6	-	-	HYPH
cord-328460-thx9zh11	326	7	dependent	dependent	JJ
cord-328460-thx9zh11	326	8	DNA	dna	NN
cord-328460-thx9zh11	326	9	unwinding	unwinding	NN
cord-328460-thx9zh11	326	10	enzyme	enzyme	NN
cord-328460-thx9zh11	326	11	Helicase	Helicase	NNP
cord-328460-thx9zh11	326	12	-	-	HYPH
cord-328460-thx9zh11	326	13	dependent	dependent	JJ
cord-328460-thx9zh11	326	14	isothermal	isothermal	JJ
cord-328460-thx9zh11	326	15	DNA	dna	NN
cord-328460-thx9zh11	326	16	amplification	amplification	NN
cord-328460-thx9zh11	326	17	Isothermal	Isothermal	NNP
cord-328460-thx9zh11	326	18	DNA	dna	NN
cord-328460-thx9zh11	326	19	amplification	amplification	NN
cord-328460-thx9zh11	326	20	in	in	IN
cord-328460-thx9zh11	326	21	vitro	vitro	FW
cord-328460-thx9zh11	326	22	:	:	:
cord-328460-thx9zh11	327	1	The	the	DT
cord-328460-thx9zh11	327	2	helicase	helicase	NN
cord-328460-thx9zh11	327	3	-	-	HYPH
cord-328460-thx9zh11	327	4	dependent	dependent	JJ
cord-328460-thx9zh11	327	5	amplification	amplification	NN
cord-328460-thx9zh11	327	6	system	system	NN
cord-328460-thx9zh11	327	7	Unraveling	unravel	VBG
cord-328460-thx9zh11	327	8	DNA	dna	NN
cord-328460-thx9zh11	327	9	helicases	helicase	NNS
cord-328460-thx9zh11	327	10	.	.	.
cord-328460-thx9zh11	328	1	Motif	motif	NN
cord-328460-thx9zh11	328	2	,	,	,
cord-328460-thx9zh11	328	3	structure	structure	NN
cord-328460-thx9zh11	328	4	,	,	,
cord-328460-thx9zh11	328	5	mechanism	mechanism	NN
cord-328460-thx9zh11	328	6	and	and	CC
cord-328460-thx9zh11	328	7	function	function	NN
cord-328460-thx9zh11	328	8	RNA	RNA	NNP
cord-328460-thx9zh11	328	9	helicases	helicase	NNS
cord-328460-thx9zh11	328	10	at	at	IN
cord-328460-thx9zh11	328	11	work	work	NN
cord-328460-thx9zh11	328	12	:	:	:
cord-328460-thx9zh11	329	1	Binding	bind	VBG
cord-328460-thx9zh11	329	2	and	and	CC
cord-328460-thx9zh11	329	3	rearranging	rearrange	VBG
cord-328460-thx9zh11	329	4	Translocation	translocation	NN
cord-328460-thx9zh11	329	5	and	and	CC
cord-328460-thx9zh11	329	6	unwinding	unwinding	NN
cord-328460-thx9zh11	329	7	mechanisms	mechanism	NNS
cord-328460-thx9zh11	329	8	of	of	IN
cord-328460-thx9zh11	329	9	RNA	RNA	NNP
cord-328460-thx9zh11	329	10	and	and	CC
cord-328460-thx9zh11	329	11	DNA	DNA	NNP
cord-328460-thx9zh11	329	12	helicases	helicase	NNS
cord-328460-thx9zh11	329	13	Helicase	Helicase	NNP
cord-328460-thx9zh11	329	14	motifs	motif	NNS
cord-328460-thx9zh11	329	15	:	:	:
cord-328460-thx9zh11	330	1	The	the	DT
cord-328460-thx9zh11	330	2	engine	engine	NN
cord-328460-thx9zh11	330	3	that	that	WDT
cord-328460-thx9zh11	330	4	powers	power	VBZ
cord-328460-thx9zh11	330	5	DNA	DNA	NNP
cord-328460-thx9zh11	330	6	unwinding	unwind	VBG
cord-328460-thx9zh11	330	7	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	330	8	coli	coli	NNS
cord-328460-thx9zh11	330	9	helicase	helicase	NNP
cord-328460-thx9zh11	330	10	II	II	NNP
cord-328460-thx9zh11	330	11	(	(	-LRB-
cord-328460-thx9zh11	330	12	uvrD	uvrD	NNP
cord-328460-thx9zh11	330	13	)	)	-RRB-
cord-328460-thx9zh11	330	14	protein	protein	NN
cord-328460-thx9zh11	330	15	can	can	MD
cord-328460-thx9zh11	330	16	completely	completely	RB
cord-328460-thx9zh11	330	17	unwind	unwind	VB
cord-328460-thx9zh11	330	18	fully	fully	RB
cord-328460-thx9zh11	330	19	duplex	duplex	JJ
cord-328460-thx9zh11	330	20	linear	linear	NN
cord-328460-thx9zh11	330	21	and	and	CC
cord-328460-thx9zh11	330	22	nicked	nick	VBD
cord-328460-thx9zh11	330	23	circular	circular	JJ
cord-328460-thx9zh11	330	24	DNA	DNA	NNP
cord-328460-thx9zh11	330	25	Interaction	Interaction	NNP
cord-328460-thx9zh11	330	26	between	between	IN
cord-328460-thx9zh11	330	27	Escherichia	Escherichia	NNP
cord-328460-thx9zh11	330	28	coli	coli	NNS
cord-328460-thx9zh11	330	29	DNA	dna	NN
cord-328460-thx9zh11	330	30	polymerase	polymerase	NN
cord-328460-thx9zh11	330	31	IV	iv	NN
cord-328460-thx9zh11	330	32	and	and	CC
cord-328460-thx9zh11	330	33	single	single	RB
cord-328460-thx9zh11	330	34	-	-	HYPH
cord-328460-thx9zh11	330	35	stranded	strand	VBN
cord-328460-thx9zh11	330	36	DNA	dna	NN
cord-328460-thx9zh11	330	37	-	-	HYPH
cord-328460-thx9zh11	330	38	binding	bind	VBG
cord-328460-thx9zh11	330	39	protein	protein	NN
cord-328460-thx9zh11	330	40	is	be	VBZ
cord-328460-thx9zh11	330	41	required	require	VBN
cord-328460-thx9zh11	330	42	for	for	IN
cord-328460-thx9zh11	330	43	DNA	dna	NN
cord-328460-thx9zh11	330	44	synthesis	synthesis	NN
cord-328460-thx9zh11	330	45	on	on	IN
cord-328460-thx9zh11	330	46	SSB	SSB	NNP
cord-328460-thx9zh11	330	47	-	-	HYPH
cord-328460-thx9zh11	330	48	coated	coated	JJ
cord-328460-thx9zh11	330	49	DNA	DNA	NNP
cord-328460-thx9zh11	330	50	SSB	SSB	NNP
cord-328460-thx9zh11	330	51	as	as	IN
cord-328460-thx9zh11	330	52	an	an	DT
cord-328460-thx9zh11	330	53	organizer	organizer	NN
cord-328460-thx9zh11	330	54	/	/	SYM
cord-328460-thx9zh11	330	55	mobilizer	mobilizer	NN
cord-328460-thx9zh11	330	56	of	of	IN
cord-328460-thx9zh11	330	57	genome	genome	NN
cord-328460-thx9zh11	330	58	maintenance	maintenance	NN
cord-328460-thx9zh11	330	59	complexes	complex	NNS
cord-328460-thx9zh11	330	60	Escherichia	escherichia	NN
cord-328460-thx9zh11	330	61	coli	coli	NNS
cord-328460-thx9zh11	331	1	MutL	MutL	NNP
cord-328460-thx9zh11	331	2	loads	load	NNS
cord-328460-thx9zh11	331	3	DNA	DNA	NNP
cord-328460-thx9zh11	331	4	helicase	helicase	NN
cord-328460-thx9zh11	331	5	II	II	NNP
cord-328460-thx9zh11	331	6	onto	onto	IN
cord-328460-thx9zh11	331	7	DNA	dna	NN
cord-328460-thx9zh11	332	1	The	the	DT
cord-328460-thx9zh11	332	2	UvrD	UvrD	NNP
cord-328460-thx9zh11	332	3	helicase	helicase	NN
cord-328460-thx9zh11	332	4	and	and	CC
cord-328460-thx9zh11	332	5	its	-PRON-	PRP$
cord-328460-thx9zh11	332	6	modulation	modulation	NN
cord-328460-thx9zh11	332	7	by	by	IN
cord-328460-thx9zh11	332	8	the	the	DT
cord-328460-thx9zh11	332	9	mismatch	mismatch	NN
cord-328460-thx9zh11	332	10	repair	repair	NN
cord-328460-thx9zh11	332	11	protein	protein	NN
cord-328460-thx9zh11	332	12	MutL	MutL	NNP
cord-328460-thx9zh11	332	13	Characterization	characterization	NN
cord-328460-thx9zh11	332	14	of	of	IN
cord-328460-thx9zh11	332	15	a	a	DT
cord-328460-thx9zh11	332	16	thermostable	thermostable	JJ
cord-328460-thx9zh11	332	17	UvrD	UvrD	NNP
cord-328460-thx9zh11	332	18	helicase	helicase	NN
cord-328460-thx9zh11	332	19	and	and	CC
cord-328460-thx9zh11	332	20	its	-PRON-	PRP$
cord-328460-thx9zh11	332	21	participation	participation	NN
cord-328460-thx9zh11	332	22	in	in	IN
cord-328460-thx9zh11	332	23	helicase	helicase	NN
cord-328460-thx9zh11	332	24	-	-	HYPH
cord-328460-thx9zh11	332	25	dependent	dependent	JJ
cord-328460-thx9zh11	332	26	amplification	amplification	NN
cord-328460-thx9zh11	332	27	Development	Development	NNP
cord-328460-thx9zh11	332	28	of	of	IN
cord-328460-thx9zh11	332	29	a	a	DT
cord-328460-thx9zh11	332	30	novel	novel	JJ
cord-328460-thx9zh11	332	31	one	one	CD
cord-328460-thx9zh11	332	32	-	-	HYPH
cord-328460-thx9zh11	332	33	tube	tube	NN
cord-328460-thx9zh11	332	34	isothermal	isothermal	JJ
cord-328460-thx9zh11	332	35	reverse	reverse	NN
cord-328460-thx9zh11	332	36	transcription	transcription	NN
cord-328460-thx9zh11	332	37	thermophilic	thermophilic	JJ
cord-328460-thx9zh11	332	38	helicase	helicase	NN
cord-328460-thx9zh11	332	39	-	-	HYPH
cord-328460-thx9zh11	332	40	dependent	dependent	JJ
cord-328460-thx9zh11	332	41	amplification	amplification	NN
cord-328460-thx9zh11	332	42	platform	platform	NN
cord-328460-thx9zh11	332	43	for	for	IN
cord-328460-thx9zh11	332	44	rapid	rapid	JJ
cord-328460-thx9zh11	332	45	RNA	RNA	NNP
cord-328460-thx9zh11	332	46	detection	detection	NN
cord-328460-thx9zh11	332	47	Development	Development	NNP
cord-328460-thx9zh11	332	48	of	of	IN
cord-328460-thx9zh11	332	49	isothermal	isothermal	JJ
cord-328460-thx9zh11	332	50	TaqMan	TaqMan	NNP
cord-328460-thx9zh11	332	51	assays	assay	NNS
cord-328460-thx9zh11	332	52	for	for	IN
cord-328460-thx9zh11	332	53	detection	detection	NN
cord-328460-thx9zh11	332	54	of	of	IN
cord-328460-thx9zh11	332	55	biothreat	biothreat	NN
cord-328460-thx9zh11	332	56	organisms	organism	NNS
cord-328460-thx9zh11	332	57	Helicase	Helicase	NNP
cord-328460-thx9zh11	332	58	dependent	dependent	JJ
cord-328460-thx9zh11	332	59	on	on	IN
cord-328460-thx9zh11	332	60	chip	chip	NN
cord-328460-thx9zh11	332	61	-	-	HYPH
cord-328460-thx9zh11	332	62	amplification	amplification	NN
cord-328460-thx9zh11	332	63	and	and	CC
cord-328460-thx9zh11	332	64	its	-PRON-	PRP$
cord-328460-thx9zh11	332	65	use	use	NN
cord-328460-thx9zh11	332	66	in	in	IN
cord-328460-thx9zh11	332	67	multiplex	multiplex	JJ
cord-328460-thx9zh11	332	68	pathogen	pathogen	NN
cord-328460-thx9zh11	332	69	detection	detection	NN
cord-328460-thx9zh11	332	70	Microfluidic	Microfluidic	NNP
cord-328460-thx9zh11	332	71	devices	device	NNS
cord-328460-thx9zh11	332	72	harboring	harbor	VBG
cord-328460-thx9zh11	332	73	unsealed	unsealed	JJ
cord-328460-thx9zh11	332	74	reactors	reactor	NNS
cord-328460-thx9zh11	332	75	for	for	IN
cord-328460-thx9zh11	332	76	real	real	JJ
cord-328460-thx9zh11	332	77	-	-	HYPH
cord-328460-thx9zh11	332	78	time	time	NN
cord-328460-thx9zh11	332	79	isothermal	isothermal	JJ
cord-328460-thx9zh11	332	80	helicase	helicase	NN
cord-328460-thx9zh11	332	81	-	-	HYPH
cord-328460-thx9zh11	332	82	dependent	dependent	JJ
cord-328460-thx9zh11	332	83	amplification	amplification	NN
cord-328460-thx9zh11	333	1	Multiple	multiple	JJ
cord-328460-thx9zh11	333	2	strategies	strategy	NNS
cord-328460-thx9zh11	333	3	to	to	TO
cord-328460-thx9zh11	333	4	improve	improve	VB
cord-328460-thx9zh11	333	5	sensitivity	sensitivity	NN
cord-328460-thx9zh11	333	6	,	,	,
cord-328460-thx9zh11	333	7	speed	speed	NN
cord-328460-thx9zh11	333	8	and	and	CC
cord-328460-thx9zh11	333	9	robustness	robustness	NN
cord-328460-thx9zh11	333	10	of	of	IN
cord-328460-thx9zh11	333	11	isothermal	isothermal	JJ
cord-328460-thx9zh11	333	12	nucleic	nucleic	JJ
cord-328460-thx9zh11	333	13	acid	acid	NN
cord-328460-thx9zh11	333	14	amplification	amplification	NN
cord-328460-thx9zh11	333	15	for	for	IN
cord-328460-thx9zh11	333	16	rapid	rapid	JJ
cord-328460-thx9zh11	333	17	pathogen	pathogen	NN
cord-328460-thx9zh11	333	18	detection	detection	NN
cord-328460-thx9zh11	333	19	An	an	DT
cord-328460-thx9zh11	333	20	integrated	integrated	JJ
cord-328460-thx9zh11	333	21	disposable	disposable	JJ
cord-328460-thx9zh11	333	22	device	device	NN
cord-328460-thx9zh11	333	23	for	for	IN
cord-328460-thx9zh11	333	24	DNA	dna	NN
cord-328460-thx9zh11	333	25	extraction	extraction	NN
cord-328460-thx9zh11	333	26	and	and	CC
cord-328460-thx9zh11	333	27	helicase	helicase	NN
cord-328460-thx9zh11	333	28	dependent	dependent	JJ
cord-328460-thx9zh11	333	29	amplification	amplification	NN
cord-328460-thx9zh11	333	30	Signal	Signal	NNP
cord-328460-thx9zh11	333	31	amplification	amplification	NN
cord-328460-thx9zh11	333	32	of	of	IN
cord-328460-thx9zh11	333	33	padlock	padlock	NN
cord-328460-thx9zh11	333	34	probes	probe	NNS
cord-328460-thx9zh11	333	35	by	by	IN
cord-328460-thx9zh11	333	36	rolling	roll	VBG
cord-328460-thx9zh11	333	37	circle	circle	NN
cord-328460-thx9zh11	333	38	replication	replication	NN
cord-328460-thx9zh11	333	39	Nucleic	Nucleic	NNP
cord-328460-thx9zh11	333	40	acid	acid	NN
cord-328460-thx9zh11	333	41	-	-	HYPH
cord-328460-thx9zh11	333	42	based	base	VBN
cord-328460-thx9zh11	333	43	methods	method	NNS
cord-328460-thx9zh11	333	44	for	for	IN
cord-328460-thx9zh11	333	45	the	the	DT
cord-328460-thx9zh11	333	46	detection	detection	NN
cord-328460-thx9zh11	333	47	of	of	IN
cord-328460-thx9zh11	333	48	bacterial	bacterial	JJ
cord-328460-thx9zh11	333	49	pathogens	pathogen	NNS
cord-328460-thx9zh11	333	50	:	:	:
cord-328460-thx9zh11	334	1	Present	present	JJ
cord-328460-thx9zh11	334	2	and	and	CC
cord-328460-thx9zh11	334	3	future	future	JJ
cord-328460-thx9zh11	334	4	considerations	consideration	NNS
cord-328460-thx9zh11	334	5	for	for	IN
cord-328460-thx9zh11	334	6	the	the	DT
cord-328460-thx9zh11	334	7	clinical	clinical	JJ
cord-328460-thx9zh11	334	8	laboratory	laboratory	NN
cord-328460-thx9zh11	334	9	Padlock	Padlock	NNP
cord-328460-thx9zh11	334	10	probes	probe	NNS
cord-328460-thx9zh11	334	11	:	:	:
cord-328460-thx9zh11	334	12	Circularizing	circularize	VBG
cord-328460-thx9zh11	334	13	oligonucleotides	oligonucleotide	NNS
cord-328460-thx9zh11	334	14	for	for	IN
cord-328460-thx9zh11	334	15	localized	localized	JJ
cord-328460-thx9zh11	334	16	DNA	dna	NN
cord-328460-thx9zh11	334	17	detection	detection	NN
cord-328460-thx9zh11	334	18	Mutation	mutation	NN
cord-328460-thx9zh11	334	19	detection	detection	NN
cord-328460-thx9zh11	334	20	and	and	CC
cord-328460-thx9zh11	334	21	single	single	JJ
cord-328460-thx9zh11	334	22	-	-	HYPH
cord-328460-thx9zh11	334	23	molecule	molecule	NN
cord-328460-thx9zh11	334	24	counting	counting	NN
cord-328460-thx9zh11	334	25	using	use	VBG
cord-328460-thx9zh11	334	26	isothermal	isothermal	JJ
cord-328460-thx9zh11	334	27	rolling	rolling	JJ
cord-328460-thx9zh11	334	28	-	-	HYPH
cord-328460-thx9zh11	334	29	circle	circle	NN
cord-328460-thx9zh11	334	30	amplification	amplification	NN
cord-328460-thx9zh11	334	31	Digital	Digital	NNP
cord-328460-thx9zh11	334	32	quantification	quantification	NN
cord-328460-thx9zh11	334	33	using	use	VBG
cord-328460-thx9zh11	334	34	amplified	amplify	VBN
cord-328460-thx9zh11	334	35	single	single	JJ
cord-328460-thx9zh11	334	36	-	-	HYPH
cord-328460-thx9zh11	334	37	molecule	molecule	NN
cord-328460-thx9zh11	334	38	detection	detection	NN
cord-328460-thx9zh11	334	39	Homogeneous	Homogeneous	NNP
cord-328460-thx9zh11	334	40	amplified	amplify	VBD
cord-328460-thx9zh11	334	41	single	single	JJ
cord-328460-thx9zh11	334	42	-	-	HYPH
cord-328460-thx9zh11	334	43	molecule	molecule	NN
cord-328460-thx9zh11	334	44	detection	detection	NN
cord-328460-thx9zh11	334	45	:	:	:
cord-328460-thx9zh11	334	46	Characterization	characterization	NN
cord-328460-thx9zh11	334	47	of	of	IN
cord-328460-thx9zh11	334	48	key	key	JJ
cord-328460-thx9zh11	334	49	parameters	parameter	NNS
cord-328460-thx9zh11	334	50	Rolling	rolling	NN
cord-328460-thx9zh11	334	51	-	-	HYPH
cord-328460-thx9zh11	334	52	circle	circle	NN
cord-328460-thx9zh11	334	53	amplification	amplification	NN
cord-328460-thx9zh11	334	54	of	of	IN
cord-328460-thx9zh11	334	55	viral	viral	JJ
cord-328460-thx9zh11	334	56	DNA	dna	NN
cord-328460-thx9zh11	334	57	genomes	genome	NNS
cord-328460-thx9zh11	334	58	using	use	VBG
cord-328460-thx9zh11	334	59	phi29	phi29	ADD
cord-328460-thx9zh11	334	60	polymerase	polymerase	NN
cord-328460-thx9zh11	334	61	Cell	cell	NN
cord-328460-thx9zh11	334	62	-	-	HYPH
cord-328460-thx9zh11	334	63	free	free	JJ
cord-328460-thx9zh11	334	64	cloning	cloning	NN
cord-328460-thx9zh11	334	65	using	use	VBG
cord-328460-thx9zh11	334	66	phi29	phi29	ADD
cord-328460-thx9zh11	334	67	DNA	dna	NN
cord-328460-thx9zh11	334	68	polymerase	polymerase	NN
cord-328460-thx9zh11	334	69	Rolling	rolling	NN
cord-328460-thx9zh11	334	70	circle	circle	NN
cord-328460-thx9zh11	334	71	amplification	amplification	NN
cord-328460-thx9zh11	334	72	and	and	CC
cord-328460-thx9zh11	334	73	circle	circle	NN
cord-328460-thx9zh11	334	74	-	-	HYPH
cord-328460-thx9zh11	334	75	to	to	IN
cord-328460-thx9zh11	334	76	-	-	HYPH
cord-328460-thx9zh11	334	77	circle	circle	NN
cord-328460-thx9zh11	334	78	amplification	amplification	NN
cord-328460-thx9zh11	334	79	of	of	IN
cord-328460-thx9zh11	334	80	a	a	DT
cord-328460-thx9zh11	334	81	specific	specific	JJ
cord-328460-thx9zh11	334	82	gene	gene	NN
cord-328460-thx9zh11	334	83	integrated	integrate	VBN
cord-328460-thx9zh11	334	84	with	with	IN
cord-328460-thx9zh11	334	85	electrophoretic	electrophoretic	JJ
cord-328460-thx9zh11	334	86	analysis	analysis	NN
cord-328460-thx9zh11	334	87	on	on	IN
cord-328460-thx9zh11	334	88	a	a	DT
cord-328460-thx9zh11	334	89	single	single	JJ
cord-328460-thx9zh11	334	90	chip	chip	NN
cord-328460-thx9zh11	334	91	Circle	circle	NN
cord-328460-thx9zh11	334	92	-	-	HYPH
cord-328460-thx9zh11	334	93	to	to	IN
cord-328460-thx9zh11	334	94	-	-	HYPH
cord-328460-thx9zh11	334	95	circle	circle	NN
cord-328460-thx9zh11	334	96	amplification	amplification	NN
cord-328460-thx9zh11	334	97	for	for	IN
cord-328460-thx9zh11	334	98	precise	precise	JJ
cord-328460-thx9zh11	334	99	and	and	CC
cord-328460-thx9zh11	334	100	sensitive	sensitive	JJ
cord-328460-thx9zh11	334	101	DNA	dna	NN
cord-328460-thx9zh11	334	102	analysis	analysis	NN
cord-328460-thx9zh11	334	103	Microchip	microchip	NN
cord-328460-thx9zh11	334	104	electrophoresis	electrophoresis	NN
cord-328460-thx9zh11	334	105	for	for	IN
cord-328460-thx9zh11	334	106	specific	specific	JJ
cord-328460-thx9zh11	334	107	gene	gene	NN
cord-328460-thx9zh11	334	108	detection	detection	NN
cord-328460-thx9zh11	334	109	of	of	IN
cord-328460-thx9zh11	334	110	the	the	DT
cord-328460-thx9zh11	334	111	pathogenic	pathogenic	JJ
cord-328460-thx9zh11	334	112	bacteria	bacteria	NNS
cord-328460-thx9zh11	334	113	V.	V.	NNP
cord-328460-thx9zh11	334	114	cholerae	cholerae	NN
cord-328460-thx9zh11	334	115	by	by	IN
cord-328460-thx9zh11	334	116	circle	circle	NN
cord-328460-thx9zh11	334	117	-	-	HYPH
cord-328460-thx9zh11	334	118	to	to	IN
cord-328460-thx9zh11	334	119	-	-	HYPH
cord-328460-thx9zh11	334	120	circle	circle	NN
cord-328460-thx9zh11	334	121	amplification	amplification	NN
cord-328460-thx9zh11	334	122	Droplet	droplet	NN
cord-328460-thx9zh11	334	123	-	-	HYPH
cord-328460-thx9zh11	334	124	based	base	VBN
cord-328460-thx9zh11	334	125	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	334	126	systems	system	NNS
cord-328460-thx9zh11	334	127	for	for	IN
cord-328460-thx9zh11	334	128	high	high	JJ
cord-328460-thx9zh11	334	129	-	-	HYPH
cord-328460-thx9zh11	334	130	throughput	throughput	NN
cord-328460-thx9zh11	334	131	single	single	JJ
cord-328460-thx9zh11	334	132	dna	dna	NN
cord-328460-thx9zh11	334	133	molecule	molecule	NN
cord-328460-thx9zh11	334	134	isothermal	isothermal	JJ
cord-328460-thx9zh11	334	135	amplification	amplification	NN
cord-328460-thx9zh11	334	136	and	and	CC
cord-328460-thx9zh11	334	137	analysis	analysis	NN
cord-328460-thx9zh11	334	138	Droplet	Droplet	NNP
cord-328460-thx9zh11	334	139	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	334	140	platform	platform	NN
cord-328460-thx9zh11	334	141	for	for	IN
cord-328460-thx9zh11	334	142	highly	highly	RB
cord-328460-thx9zh11	334	143	sensitive	sensitive	JJ
cord-328460-thx9zh11	334	144	and	and	CC
cord-328460-thx9zh11	334	145	quantitative	quantitative	JJ
cord-328460-thx9zh11	334	146	detection	detection	NN
cord-328460-thx9zh11	334	147	of	of	IN
cord-328460-thx9zh11	334	148	malaria	malaria	NN
cord-328460-thx9zh11	334	149	-	-	HYPH
cord-328460-thx9zh11	334	150	causing	cause	VBG
cord-328460-thx9zh11	334	151	plasmodium	plasmodium	NN
cord-328460-thx9zh11	334	152	parasites	parasite	NNS
cord-328460-thx9zh11	334	153	based	base	VBN
cord-328460-thx9zh11	334	154	on	on	IN
cord-328460-thx9zh11	334	155	enzyme	enzyme	NN
cord-328460-thx9zh11	334	156	activity	activity	NN
cord-328460-thx9zh11	334	157	measurement	measurement	NN
cord-328460-thx9zh11	334	158	Comprehensive	Comprehensive	NNP
cord-328460-thx9zh11	334	159	human	human	JJ
cord-328460-thx9zh11	334	160	genome	genome	NN
cord-328460-thx9zh11	334	161	amplification	amplification	NN
cord-328460-thx9zh11	334	162	using	use	VBG
cord-328460-thx9zh11	334	163	multiple	multiple	JJ
cord-328460-thx9zh11	334	164	displacement	displacement	NN
cord-328460-thx9zh11	334	165	amplification	amplification	NN
cord-328460-thx9zh11	334	166	Highly	highly	RB
cord-328460-thx9zh11	334	167	efficient	efficient	JJ
cord-328460-thx9zh11	334	168	DNA	dna	NN
cord-328460-thx9zh11	334	169	synthesis	synthesis	NN
cord-328460-thx9zh11	334	170	by	by	IN
cord-328460-thx9zh11	334	171	the	the	DT
cord-328460-thx9zh11	334	172	phage	phage	NN
cord-328460-thx9zh11	334	173	phi	phi	NNP
cord-328460-thx9zh11	334	174	29	29	CD
cord-328460-thx9zh11	334	175	DNA	dna	NN
cord-328460-thx9zh11	334	176	polymerase	polymerase	NN
cord-328460-thx9zh11	334	177	.	.	.
cord-328460-thx9zh11	335	1	Symmetrical	symmetrical	JJ
cord-328460-thx9zh11	335	2	mode	mode	NN
cord-328460-thx9zh11	335	3	of	of	IN
cord-328460-thx9zh11	335	4	DNA	dna	NN
cord-328460-thx9zh11	335	5	replication	replication	NN
cord-328460-thx9zh11	335	6	TempliPhi	TempliPhi	NNP
cord-328460-thx9zh11	335	7	,	,	,
cord-328460-thx9zh11	335	8	phi29	phi29	NN
cord-328460-thx9zh11	335	9	DNA	dna	NN
cord-328460-thx9zh11	335	10	polymerase	polymerase	NN
cord-328460-thx9zh11	335	11	based	base	VBN
cord-328460-thx9zh11	335	12	rolling	roll	VBG
cord-328460-thx9zh11	335	13	circle	circle	NN
cord-328460-thx9zh11	335	14	amplification	amplification	NN
cord-328460-thx9zh11	335	15	of	of	IN
cord-328460-thx9zh11	335	16	templates	template	NNS
cord-328460-thx9zh11	335	17	for	for	IN
cord-328460-thx9zh11	335	18	DNA	dna	NN
cord-328460-thx9zh11	335	19	sequencing	sequencing	NN
cord-328460-thx9zh11	335	20	Optimization	optimization	NN
cord-328460-thx9zh11	335	21	of	of	IN
cord-328460-thx9zh11	335	22	the	the	DT
cord-328460-thx9zh11	335	23	polymerase	polymerase	NN
cord-328460-thx9zh11	335	24	chain	chain	NN
cord-328460-thx9zh11	335	25	reaction	reaction	NN
cord-328460-thx9zh11	335	26	with	with	IN
cord-328460-thx9zh11	335	27	regard	regard	NN
cord-328460-thx9zh11	335	28	to	to	IN
cord-328460-thx9zh11	335	29	fidelity	fidelity	NN
cord-328460-thx9zh11	335	30	:	:	:
cord-328460-thx9zh11	335	31	Modified	modify	VBN
cord-328460-thx9zh11	335	32	T7	T7	NNP
cord-328460-thx9zh11	335	33	,	,	,
cord-328460-thx9zh11	335	34	Taq	Taq	NNP
cord-328460-thx9zh11	335	35	,	,	,
cord-328460-thx9zh11	335	36	and	and	CC
cord-328460-thx9zh11	335	37	vent	vent	NN
cord-328460-thx9zh11	335	38	DNA	dna	NN
cord-328460-thx9zh11	335	39	polymerases	polymerase	NNS
cord-328460-thx9zh11	335	40	Fidelity	Fidelity	NNP
cord-328460-thx9zh11	335	41	of	of	IN
cord-328460-thx9zh11	335	42	DNA	DNA	NNP
cord-328460-thx9zh11	335	43	polymerases	polymerase	NNS
cord-328460-thx9zh11	335	44	in	in	IN
cord-328460-thx9zh11	335	45	DNA	dna	NN
cord-328460-thx9zh11	335	46	amplification	amplification	NN
cord-328460-thx9zh11	335	47	Unbiased	unbiased	JJ
cord-328460-thx9zh11	335	48	whole	whole	JJ
cord-328460-thx9zh11	335	49	genome	genome	NN
cord-328460-thx9zh11	335	50	amplification	amplification	NN
cord-328460-thx9zh11	335	51	directly	directly	RB
cord-328460-thx9zh11	335	52	from	from	IN
cord-328460-thx9zh11	335	53	clinical	clinical	JJ
cord-328460-thx9zh11	335	54	samples	sample	NNS
cord-328460-thx9zh11	335	55	Genomic	genomic	JJ
cord-328460-thx9zh11	335	56	DNA	dna	NN
cord-328460-thx9zh11	335	57	amplification	amplification	NN
cord-328460-thx9zh11	335	58	from	from	IN
cord-328460-thx9zh11	335	59	a	a	DT
cord-328460-thx9zh11	335	60	single	single	JJ
cord-328460-thx9zh11	335	61	bacterium	bacterium	NN
cord-328460-thx9zh11	336	1	Nanoliter	Nanoliter	NNP
cord-328460-thx9zh11	336	2	reactors	reactor	NNS
cord-328460-thx9zh11	336	3	improve	improve	VBP
cord-328460-thx9zh11	336	4	multiple	multiple	JJ
cord-328460-thx9zh11	336	5	displacement	displacement	NN
cord-328460-thx9zh11	336	6	amplification	amplification	NN
cord-328460-thx9zh11	336	7	of	of	IN
cord-328460-thx9zh11	336	8	genomes	genome	NNS
cord-328460-thx9zh11	336	9	from	from	IN
cord-328460-thx9zh11	336	10	single	single	JJ
cord-328460-thx9zh11	336	11	cells	cell	NNS
cord-328460-thx9zh11	336	12	Single	Single	NNP
cord-328460-thx9zh11	336	13	-	-	HYPH
cord-328460-thx9zh11	336	14	Molecule	molecule	NN
cord-328460-thx9zh11	336	15	DNA	dna	NN
cord-328460-thx9zh11	336	16	Amplification	amplification	NN
cord-328460-thx9zh11	336	17	and	and	CC
cord-328460-thx9zh11	336	18	analysis	analysis	NN
cord-328460-thx9zh11	336	19	using	use	VBG
cord-328460-thx9zh11	336	20	microfluidics	microfluidic	NNS
cord-328460-thx9zh11	336	21	DNA	dna	NN
cord-328460-thx9zh11	336	22	detection	detection	NN
cord-328460-thx9zh11	336	23	using	use	VBG
cord-328460-thx9zh11	336	24	recombination	recombination	NN
cord-328460-thx9zh11	336	25	proteins	protein	NNS
cord-328460-thx9zh11	337	1	A	a	DT
cord-328460-thx9zh11	337	2	phaseguided	phaseguided	JJ
cord-328460-thx9zh11	337	3	passive	passive	JJ
cord-328460-thx9zh11	337	4	batch	batch	NN
cord-328460-thx9zh11	337	5	microfluidic	microfluidic	NNP
cord-328460-thx9zh11	337	6	mixing	mix	VBG
cord-328460-thx9zh11	337	7	chamber	chamber	NN
cord-328460-thx9zh11	337	8	for	for	IN
cord-328460-thx9zh11	337	9	isothermal	isothermal	JJ
cord-328460-thx9zh11	337	10	amplification	amplification	NN
cord-328460-thx9zh11	337	11	Phaseguides	Phaseguides	NNP
cord-328460-thx9zh11	337	12	:	:	:
cord-328460-thx9zh11	338	1	A	a	DT
cord-328460-thx9zh11	338	2	paradigm	paradigm	NN
cord-328460-thx9zh11	338	3	shift	shift	NN
cord-328460-thx9zh11	338	4	in	in	IN
cord-328460-thx9zh11	338	5	microfluidic	microfluidic	JJ
cord-328460-thx9zh11	338	6	priming	priming	NN
cord-328460-thx9zh11	338	7	and	and	CC
cord-328460-thx9zh11	338	8	emptying	emptying	NN
cord-328460-thx9zh11	339	1	Hot	hot	JJ
cord-328460-thx9zh11	339	2	start	start	NN
cord-328460-thx9zh11	340	1	PCR	PCR	NNP
cord-328460-thx9zh11	340	2	Digital	Digital	NNP
cord-328460-thx9zh11	340	3	isothermal	isothermal	JJ
cord-328460-thx9zh11	340	4	quantification	quantification	NN
cord-328460-thx9zh11	340	5	of	of	IN
cord-328460-thx9zh11	340	6	nucleic	nucleic	JJ
cord-328460-thx9zh11	340	7	acids	acid	NNS
cord-328460-thx9zh11	340	8	via	via	IN
cord-328460-thx9zh11	340	9	simultaneous	simultaneous	JJ
cord-328460-thx9zh11	340	10	chemical	chemical	NN
cord-328460-thx9zh11	340	11	initiation	initiation	NN
cord-328460-thx9zh11	340	12	of	of	IN
cord-328460-thx9zh11	340	13	recombinase	recombinase	NNP
cord-328460-thx9zh11	340	14	polymerase	polymerase	NN
cord-328460-thx9zh11	340	15	amplification	amplification	NN
cord-328460-thx9zh11	340	16	reactions	reaction	NNS
cord-328460-thx9zh11	340	17	on	on	IN
cord-328460-thx9zh11	340	18	SlipChip	SlipChip	NNP
cord-328460-thx9zh11	340	19	Nucleic	Nucleic	NNP
cord-328460-thx9zh11	340	20	acid	acid	NN
cord-328460-thx9zh11	340	21	sequence	sequence	NN
cord-328460-thx9zh11	340	22	-	-	HYPH
cord-328460-thx9zh11	340	23	based	base	VBN
cord-328460-thx9zh11	340	24	amplification	amplification	NN
cord-328460-thx9zh11	340	25	Characteristics	characteristic	NNS
cord-328460-thx9zh11	340	26	and	and	CC
cord-328460-thx9zh11	340	27	applications	application	NNS
cord-328460-thx9zh11	340	28	of	of	IN
cord-328460-thx9zh11	340	29	nucleic	nucleic	JJ
cord-328460-thx9zh11	340	30	acid	acid	NN
cord-328460-thx9zh11	340	31	sequence	sequence	NN
cord-328460-thx9zh11	340	32	-	-	HYPH
cord-328460-thx9zh11	340	33	based	base	VBN
cord-328460-thx9zh11	340	34	amplification	amplification	NN
cord-328460-thx9zh11	340	35	(	(	-LRB-
cord-328460-thx9zh11	340	36	NASBA	nasba	NN
cord-328460-thx9zh11	340	37	)	)	-RRB-
cord-328460-thx9zh11	341	1	Quantitative	quantitative	JJ
cord-328460-thx9zh11	341	2	detection	detection	NN
cord-328460-thx9zh11	341	3	of	of	IN
cord-328460-thx9zh11	341	4	hepatitis	hepatitis	NN
cord-328460-thx9zh11	341	5	B	B	NNP
cord-328460-thx9zh11	341	6	virus	virus	NN
cord-328460-thx9zh11	341	7	DNA	dna	NN
cord-328460-thx9zh11	341	8	by	by	IN
cord-328460-thx9zh11	341	9	real	real	JJ
cord-328460-thx9zh11	341	10	-	-	HYPH
cord-328460-thx9zh11	341	11	time	time	NN
cord-328460-thx9zh11	341	12	nucleic	nucleic	JJ
cord-328460-thx9zh11	341	13	acid	acid	NN
cord-328460-thx9zh11	341	14	sequence	sequence	NN
cord-328460-thx9zh11	341	15	-	-	HYPH
cord-328460-thx9zh11	341	16	based	base	VBN
cord-328460-thx9zh11	341	17	amplification	amplification	NN
cord-328460-thx9zh11	341	18	with	with	IN
cord-328460-thx9zh11	341	19	molecular	molecular	JJ
cord-328460-thx9zh11	341	20	beacon	beacon	NN
cord-328460-thx9zh11	341	21	detection	detection	NN
cord-328460-thx9zh11	342	1	A	a	DT
cord-328460-thx9zh11	342	2	one	one	CD
cord-328460-thx9zh11	342	3	-	-	HYPH
cord-328460-thx9zh11	342	4	tube	tube	NN
cord-328460-thx9zh11	342	5	quantitative	quantitative	JJ
cord-328460-thx9zh11	342	6	HIV-1	HIV-1	NNP
cord-328460-thx9zh11	342	7	RNA	RNA	NNP
cord-328460-thx9zh11	342	8	NASBA	NASBA	NNP
cord-328460-thx9zh11	342	9	nucleic	nucleic	JJ
cord-328460-thx9zh11	342	10	acid	acid	NN
cord-328460-thx9zh11	342	11	amplification	amplification	NN
cord-328460-thx9zh11	342	12	assay	assay	NN
cord-328460-thx9zh11	342	13	using	use	VBG
cord-328460-thx9zh11	342	14	electrochemiluminiscent	electrochemiluminiscent	NN
cord-328460-thx9zh11	342	15	(	(	-LRB-
cord-328460-thx9zh11	342	16	ECL	ECL	NNP
cord-328460-thx9zh11	342	17	)	)	-RRB-
cord-328460-thx9zh11	342	18	labelled	label	VBN
cord-328460-thx9zh11	342	19	probes	probe	NNS
cord-328460-thx9zh11	342	20	Comparison	comparison	NN
cord-328460-thx9zh11	342	21	of	of	IN
cord-328460-thx9zh11	342	22	nucleic	nucleic	JJ
cord-328460-thx9zh11	342	23	acid	acid	NN
cord-328460-thx9zh11	342	24	-	-	HYPH
cord-328460-thx9zh11	342	25	based	base	VBN
cord-328460-thx9zh11	342	26	detection	detection	NN
cord-328460-thx9zh11	342	27	of	of	IN
cord-328460-thx9zh11	342	28	avian	avian	JJ
cord-328460-thx9zh11	342	29	influenza	influenza	NN
cord-328460-thx9zh11	342	30	H5N1	H5N1	NNP
cord-328460-thx9zh11	342	31	with	with	IN
cord-328460-thx9zh11	342	32	virus	virus	NN
cord-328460-thx9zh11	342	33	isolation	isolation	NN
cord-328460-thx9zh11	342	34	Human	human	JJ
cord-328460-thx9zh11	342	35	pathogenic	pathogenic	JJ
cord-328460-thx9zh11	342	36	Cryptosporidium	Cryptosporidium	NNP
cord-328460-thx9zh11	342	37	species	specie	NNS
cord-328460-thx9zh11	342	38	bioanalytical	bioanalytical	JJ
cord-328460-thx9zh11	342	39	detection	detection	NN
cord-328460-thx9zh11	342	40	method	method	NN
cord-328460-thx9zh11	342	41	with	with	IN
cord-328460-thx9zh11	342	42	single	single	JJ
cord-328460-thx9zh11	342	43	oocyst	oocyst	JJ
cord-328460-thx9zh11	342	44	detection	detection	NN
cord-328460-thx9zh11	342	45	capability	capability	NN
cord-328460-thx9zh11	342	46	PMMA	PMMA	NNP
cord-328460-thx9zh11	342	47	biosensor	biosensor	NN
cord-328460-thx9zh11	342	48	for	for	IN
cord-328460-thx9zh11	342	49	nucleic	nucleic	JJ
cord-328460-thx9zh11	342	50	acids	acid	NNS
cord-328460-thx9zh11	342	51	with	with	IN
cord-328460-thx9zh11	342	52	integrated	integrated	JJ
cord-328460-thx9zh11	342	53	mixer	mixer	NN
cord-328460-thx9zh11	342	54	and	and	CC
cord-328460-thx9zh11	342	55	electrochemical	electrochemical	JJ
cord-328460-thx9zh11	342	56	detection	detection	NN
cord-328460-thx9zh11	342	57	Strategy	Strategy	NNP
cord-328460-thx9zh11	342	58	for	for	IN
cord-328460-thx9zh11	342	59	molecular	molecular	JJ
cord-328460-thx9zh11	342	60	beacon	beacon	NN
cord-328460-thx9zh11	342	61	binding	binding	NN
cord-328460-thx9zh11	342	62	readout	readout	NN
cord-328460-thx9zh11	342	63	:	:	:
cord-328460-thx9zh11	343	1	Separating	separate	VBG
cord-328460-thx9zh11	343	2	molecular	molecular	JJ
cord-328460-thx9zh11	343	3	recognition	recognition	NN
cord-328460-thx9zh11	343	4	element	element	NN
cord-328460-thx9zh11	343	5	and	and	CC
cord-328460-thx9zh11	343	6	signal	signal	NN
cord-328460-thx9zh11	343	7	reporter	reporter	NN
cord-328460-thx9zh11	343	8	Molecular	Molecular	NNP
cord-328460-thx9zh11	343	9	engineering	engineering	NN
cord-328460-thx9zh11	343	10	of	of	IN
cord-328460-thx9zh11	343	11	DNA	dna	NN
cord-328460-thx9zh11	343	12	:	:	:
cord-328460-thx9zh11	344	1	Molecular	molecular	JJ
cord-328460-thx9zh11	344	2	beacons	beacon	NNS
cord-328460-thx9zh11	344	3	Real	real	JJ
cord-328460-thx9zh11	344	4	-	-	HYPH
cord-328460-thx9zh11	344	5	time	time	NN
cord-328460-thx9zh11	344	6	molecular	molecular	JJ
cord-328460-thx9zh11	344	7	beacon	beacon	NN
cord-328460-thx9zh11	344	8	NASBA	NASBA	NNP
cord-328460-thx9zh11	344	9	reveals	reveal	VBZ
cord-328460-thx9zh11	344	10	hblc	hblc	JJ
cord-328460-thx9zh11	344	11	expression	expression	NN
cord-328460-thx9zh11	344	12	from	from	IN
cord-328460-thx9zh11	344	13	Bacillus	Bacillus	NNP
cord-328460-thx9zh11	344	14	spp	spp	NNS
cord-328460-thx9zh11	344	15	.	.	.
cord-328460-thx9zh11	345	1	in	in	IN
cord-328460-thx9zh11	345	2	milk	milk	NN
cord-328460-thx9zh11	345	3	A	a	DT
cord-328460-thx9zh11	345	4	molecular	molecular	JJ
cord-328460-thx9zh11	345	5	beacon	beacon	NN
cord-328460-thx9zh11	345	6	-	-	HYPH
cord-328460-thx9zh11	345	7	based	base	VBN
cord-328460-thx9zh11	345	8	real	real	JJ
cord-328460-thx9zh11	345	9	time	time	NN
cord-328460-thx9zh11	345	10	NASBA	NASBA	NNP
cord-328460-thx9zh11	345	11	assay	assay	NN
cord-328460-thx9zh11	345	12	for	for	IN
cord-328460-thx9zh11	345	13	detection	detection	NN
cord-328460-thx9zh11	345	14	of	of	IN
cord-328460-thx9zh11	345	15	Listeria	Listeria	NNP
cord-328460-thx9zh11	345	16	monocytogenes	monocytogene	NNS
cord-328460-thx9zh11	345	17	in	in	IN
cord-328460-thx9zh11	345	18	food	food	NN
cord-328460-thx9zh11	345	19	products	product	NNS
cord-328460-thx9zh11	345	20	:	:	:
cord-328460-thx9zh11	345	21	Role	role	NN
cord-328460-thx9zh11	345	22	of	of	IN
cord-328460-thx9zh11	345	23	target	target	NN
cord-328460-thx9zh11	345	24	mRNA	mrna	NN
cord-328460-thx9zh11	345	25	secondary	secondary	JJ
cord-328460-thx9zh11	345	26	structure	structure	NN
cord-328460-thx9zh11	345	27	on	on	IN
cord-328460-thx9zh11	345	28	NASBA	NASBA	NNP
cord-328460-thx9zh11	345	29	design	design	NN
cord-328460-thx9zh11	345	30	Real	real	JJ
cord-328460-thx9zh11	345	31	-	-	HYPH
cord-328460-thx9zh11	345	32	time	time	NN
cord-328460-thx9zh11	345	33	nucleic	nucleic	JJ
cord-328460-thx9zh11	345	34	acid	acid	NN
cord-328460-thx9zh11	345	35	sequence	sequence	NN
cord-328460-thx9zh11	345	36	-	-	HYPH
cord-328460-thx9zh11	345	37	based	base	VBN
cord-328460-thx9zh11	345	38	amplification	amplification	NN
cord-328460-thx9zh11	345	39	in	in	IN
cord-328460-thx9zh11	345	40	nanoliter	nanoliter	JJ
cord-328460-thx9zh11	345	41	volumes	volume	NNS
cord-328460-thx9zh11	345	42	Sirevåg	Sirevåg	NNP
cord-328460-thx9zh11	345	43	,	,	,
cord-328460-thx9zh11	346	1	R.	R.	NNP
cord-328460-thx9zh11	346	2	Parallel	Parallel	NNP
cord-328460-thx9zh11	346	3	nanoliter	nanoliter	JJ
cord-328460-thx9zh11	346	4	detection	detection	NN
cord-328460-thx9zh11	346	5	of	of	IN
cord-328460-thx9zh11	346	6	cancer	cancer	NN
cord-328460-thx9zh11	346	7	markers	marker	NNS
cord-328460-thx9zh11	346	8	using	use	VBG
cord-328460-thx9zh11	346	9	polymer	polymer	NN
cord-328460-thx9zh11	346	10	microchips	microchip	NNS
cord-328460-thx9zh11	346	11	Towards	towards	IN
cord-328460-thx9zh11	346	12	a	a	DT
cord-328460-thx9zh11	346	13	"	"	``
cord-328460-thx9zh11	346	14	sample	sample	NN
cord-328460-thx9zh11	346	15	-	-	HYPH
cord-328460-thx9zh11	346	16	in	in	NN
cord-328460-thx9zh11	346	17	,	,	,
cord-328460-thx9zh11	346	18	answer	answer	VB
cord-328460-thx9zh11	346	19	-	-	HYPH
cord-328460-thx9zh11	346	20	out	out	RP
cord-328460-thx9zh11	346	21	"	"	''
cord-328460-thx9zh11	346	22	point	point	NN
cord-328460-thx9zh11	346	23	-	-	HYPH
cord-328460-thx9zh11	346	24	of	of	IN
cord-328460-thx9zh11	346	25	-	-	HYPH
cord-328460-thx9zh11	346	26	care	care	NN
cord-328460-thx9zh11	346	27	platformfor	platformfor	NN
cord-328460-thx9zh11	346	28	nucleic	nucleic	JJ
cord-328460-thx9zh11	346	29	acid	acid	NN
cord-328460-thx9zh11	346	30	extraction	extraction	NN
cord-328460-thx9zh11	346	31	and	and	CC
cord-328460-thx9zh11	346	32	amplification	amplification	NN
cord-328460-thx9zh11	346	33	:	:	:
cord-328460-thx9zh11	347	1	Using	use	VBG
cord-328460-thx9zh11	347	2	an	an	DT
cord-328460-thx9zh11	347	3	HPV	HPV	NNP
cord-328460-thx9zh11	347	4	E6	E6	NNP
cord-328460-thx9zh11	347	5	/	/	SYM
cord-328460-thx9zh11	347	6	E7mRNAModel	E7mRNAModel	NNP
cord-328460-thx9zh11	347	7	System	System	NNP
cord-328460-thx9zh11	347	8	Integrated	Integrated	NNP
cord-328460-thx9zh11	347	9	microfluidic	microfluidic	NNP
cord-328460-thx9zh11	347	10	tmRNA	tmrna	NN
cord-328460-thx9zh11	347	11	purification	purification	NN
cord-328460-thx9zh11	347	12	and	and	CC
cord-328460-thx9zh11	347	13	real	real	JJ
cord-328460-thx9zh11	347	14	-	-	HYPH
cord-328460-thx9zh11	347	15	time	time	NN
cord-328460-thx9zh11	347	16	NASBA	nasba	NN
cord-328460-thx9zh11	347	17	device	device	NN
cord-328460-thx9zh11	347	18	for	for	IN
cord-328460-thx9zh11	347	19	molecular	molecular	JJ
cord-328460-thx9zh11	347	20	diagnostics	diagnostic	NNS
cord-328460-thx9zh11	347	21	Compatible	compatible	JJ
cord-328460-thx9zh11	347	22	immuno	immuno	JJ
cord-328460-thx9zh11	347	23	-	-	HYPH
cord-328460-thx9zh11	347	24	NASBA	nasba	JJ
cord-328460-thx9zh11	347	25	LOC	loc	NN
cord-328460-thx9zh11	347	26	device	device	NN
cord-328460-thx9zh11	347	27	for	for	IN
cord-328460-thx9zh11	347	28	quantitative	quantitative	JJ
cord-328460-thx9zh11	347	29	detection	detection	NN
cord-328460-thx9zh11	347	30	of	of	IN
cord-328460-thx9zh11	347	31	waterborne	waterborne	JJ
cord-328460-thx9zh11	347	32	pathogens	pathogen	NNS
cord-328460-thx9zh11	347	33	:	:	:
cord-328460-thx9zh11	347	34	Design	design	NN
cord-328460-thx9zh11	347	35	and	and	CC
cord-328460-thx9zh11	347	36	validation	validation	NN
cord-328460-thx9zh11	347	37	This	this	DT
cord-328460-thx9zh11	347	38	article	article	NN
cord-328460-thx9zh11	347	39	is	be	VBZ
cord-328460-thx9zh11	347	40	an	an	DT
cord-328460-thx9zh11	347	41	open	open	JJ
cord-328460-thx9zh11	347	42	access	access	NN
cord-328460-thx9zh11	347	43	article	article	NN
cord-328460-thx9zh11	347	44	distributed	distribute	VBN
cord-328460-thx9zh11	347	45	under	under	IN
cord-328460-thx9zh11	347	46	the	the	DT
cord-328460-thx9zh11	347	47	terms	term	NNS
cord-328460-thx9zh11	347	48	and	and	CC
cord-328460-thx9zh11	347	49	conditions	condition	NNS
cord-328460-thx9zh11	347	50	of	of	IN
cord-328460-thx9zh11	347	51	the	the	DT
cord-328460-thx9zh11	347	52	Creative	Creative	NNP
cord-328460-thx9zh11	347	53	Commons	Commons	NNP
cord-328460-thx9zh11	347	54	Attribution	Attribution	NNP
cord-328460-thx9zh11	347	55	license	license	NN
cord-328460-thx9zh11	347	56	We	-PRON-	PRP
cord-328460-thx9zh11	347	57	acknowledge	acknowledge	VBP
cord-328460-thx9zh11	347	58	support	support	NN
cord-328460-thx9zh11	347	59	from	from	IN
cord-328460-thx9zh11	347	60	MIUR	MIUR	NNP
cord-328460-thx9zh11	347	61	(	(	-LRB-
cord-328460-thx9zh11	347	62	PRIN	PRIN	NNP
cord-328460-thx9zh11	347	63	20093N774P	20093N774P	NNP
cord-328460-thx9zh11	347	64	,	,	,
cord-328460-thx9zh11	347	65	FIRB	FIRB	NNP
cord-328460-thx9zh11	347	66	RBPR05JH2P	RBPR05JH2P	HYPH
cord-328460-thx9zh11	347	67	ITALNANONET	ITALNANONET	NNP
cord-328460-thx9zh11	347	68	RINAME	RINAME	NNP
cord-328460-thx9zh11	347	69	RBAP114AMK	RBAP114AMK	NNP
cord-328460-thx9zh11	347	70	)	)	-RRB-
cord-328460-thx9zh11	347	71	.	.	.