id sid tid token lemma pos cord-304685-s0bfhwtn 1 1 key key NN cord-304685-s0bfhwtn 1 2 : : : cord-304685-s0bfhwtn 1 3 cord-304685-s0bfhwtn cord-304685-s0bfhwtn JJ cord-304685-s0bfhwtn 1 4 authors author NNS cord-304685-s0bfhwtn 1 5 : : : cord-304685-s0bfhwtn 2 1 nan nan NNP cord-304685-s0bfhwtn 2 2 title title NN cord-304685-s0bfhwtn 2 3 : : : cord-304685-s0bfhwtn 2 4 Susceptibility susceptibility NN cord-304685-s0bfhwtn 2 5 to to IN cord-304685-s0bfhwtn 2 6 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 2 7 T t NN cord-304685-s0bfhwtn 2 8 lymphocyte lymphocyte NN cord-304685-s0bfhwtn 2 9 - - HYPH cord-304685-s0bfhwtn 2 10 induced induce VBN cord-304685-s0bfhwtn 2 11 apoptosis apoptosis NN cord-304685-s0bfhwtn 2 12 is be VBZ cord-304685-s0bfhwtn 2 13 a a DT cord-304685-s0bfhwtn 2 14 function function NN cord-304685-s0bfhwtn 2 15 of of IN cord-304685-s0bfhwtn 2 16 the the DT cord-304685-s0bfhwtn 2 17 proliferative proliferative JJ cord-304685-s0bfhwtn 2 18 status status NN cord-304685-s0bfhwtn 2 19 of of IN cord-304685-s0bfhwtn 2 20 the the DT cord-304685-s0bfhwtn 2 21 target target NN cord-304685-s0bfhwtn 2 22 date date NN cord-304685-s0bfhwtn 2 23 : : : cord-304685-s0bfhwtn 2 24 1994 1994 CD cord-304685-s0bfhwtn 2 25 - - SYM cord-304685-s0bfhwtn 2 26 02 02 CD cord-304685-s0bfhwtn 2 27 - - HYPH cord-304685-s0bfhwtn 2 28 01 01 CD cord-304685-s0bfhwtn 2 29 journal journal NN cord-304685-s0bfhwtn 2 30 : : : cord-304685-s0bfhwtn 3 1 J J NNP cord-304685-s0bfhwtn 3 2 Exp Exp NNP cord-304685-s0bfhwtn 3 3 Med Med NNP cord-304685-s0bfhwtn 3 4 DOI DOI NNP cord-304685-s0bfhwtn 4 1 : : : cord-304685-s0bfhwtn 4 2 nan nan NNP cord-304685-s0bfhwtn 4 3 sha sha NNP cord-304685-s0bfhwtn 4 4 : : : cord-304685-s0bfhwtn 4 5 8c4233100da83790ea6544851b543977da15a4ca 8c4233100da83790ea6544851b543977da15a4ca CD cord-304685-s0bfhwtn 4 6 doc_id doc_id CD cord-304685-s0bfhwtn 4 7 : : : cord-304685-s0bfhwtn 5 1 304685 304685 CD cord-304685-s0bfhwtn 5 2 cord_uid cord_uid NN cord-304685-s0bfhwtn 5 3 : : : cord-304685-s0bfhwtn 6 1 s0bfhwtn s0bfhwtn NNP cord-304685-s0bfhwtn 6 2 Cytotoxic Cytotoxic NNP cord-304685-s0bfhwtn 6 3 T t NN cord-304685-s0bfhwtn 6 4 lymphocytes lymphocyte NNS cord-304685-s0bfhwtn 6 5 ( ( -LRB- cord-304685-s0bfhwtn 6 6 CTL CTL NNP cord-304685-s0bfhwtn 6 7 ) ) -RRB- cord-304685-s0bfhwtn 6 8 kill kill VBP cord-304685-s0bfhwtn 6 9 cells cell NNS cord-304685-s0bfhwtn 6 10 by by IN cord-304685-s0bfhwtn 6 11 perturbing perturb VBG cord-304685-s0bfhwtn 6 12 the the DT cord-304685-s0bfhwtn 6 13 target target NN cord-304685-s0bfhwtn 6 14 's 's POS cord-304685-s0bfhwtn 6 15 plasma plasma NN cord-304685-s0bfhwtn 6 16 membrane membrane NN cord-304685-s0bfhwtn 6 17 and and CC cord-304685-s0bfhwtn 6 18 by by IN cord-304685-s0bfhwtn 6 19 inducing induce VBG cord-304685-s0bfhwtn 6 20 the the DT cord-304685-s0bfhwtn 6 21 disintegration disintegration NN cord-304685-s0bfhwtn 6 22 of of IN cord-304685-s0bfhwtn 6 23 the the DT cord-304685-s0bfhwtn 6 24 target target NN cord-304685-s0bfhwtn 6 25 cell cell NN cord-304685-s0bfhwtn 6 26 's 's POS cord-304685-s0bfhwtn 6 27 DNA dna NN cord-304685-s0bfhwtn 6 28 into into IN cord-304685-s0bfhwtn 6 29 oligonucleosomal oligonucleosomal JJ cord-304685-s0bfhwtn 6 30 fragments fragment NNS cord-304685-s0bfhwtn 6 31 , , , cord-304685-s0bfhwtn 6 32 a a DT cord-304685-s0bfhwtn 6 33 process process NN cord-304685-s0bfhwtn 6 34 characteristic characteristic NN cord-304685-s0bfhwtn 6 35 of of IN cord-304685-s0bfhwtn 6 36 apoptosis apoptosis NN cord-304685-s0bfhwtn 6 37 . . . cord-304685-s0bfhwtn 7 1 We -PRON- PRP cord-304685-s0bfhwtn 7 2 show show VBP cord-304685-s0bfhwtn 7 3 that that IN cord-304685-s0bfhwtn 7 4 the the DT cord-304685-s0bfhwtn 7 5 DNA dna NN cord-304685-s0bfhwtn 7 6 fragmentation fragmentation NN cord-304685-s0bfhwtn 7 7 event event NN cord-304685-s0bfhwtn 7 8 is be VBZ cord-304685-s0bfhwtn 7 9 distinct distinct JJ cord-304685-s0bfhwtn 7 10 from from IN cord-304685-s0bfhwtn 7 11 the the DT cord-304685-s0bfhwtn 7 12 membrane membrane NN cord-304685-s0bfhwtn 7 13 lysis lysis NN cord-304685-s0bfhwtn 7 14 event event NN cord-304685-s0bfhwtn 7 15 and and CC cord-304685-s0bfhwtn 7 16 is be VBZ cord-304685-s0bfhwtn 7 17 dependent dependent JJ cord-304685-s0bfhwtn 7 18 on on IN cord-304685-s0bfhwtn 7 19 the the DT cord-304685-s0bfhwtn 7 20 state state NN cord-304685-s0bfhwtn 7 21 of of IN cord-304685-s0bfhwtn 7 22 target target NN cord-304685-s0bfhwtn 7 23 cell cell NN cord-304685-s0bfhwtn 7 24 activation activation NN cord-304685-s0bfhwtn 7 25 or or CC cord-304685-s0bfhwtn 7 26 commitment commitment NN cord-304685-s0bfhwtn 7 27 into into IN cord-304685-s0bfhwtn 7 28 the the DT cord-304685-s0bfhwtn 7 29 mitotic mitotic JJ cord-304685-s0bfhwtn 7 30 cycle cycle NN cord-304685-s0bfhwtn 7 31 . . . cord-304685-s0bfhwtn 8 1 Quiescent quiescent JJ cord-304685-s0bfhwtn 8 2 cells cell NNS cord-304685-s0bfhwtn 8 3 were be VBD cord-304685-s0bfhwtn 8 4 refractory refractory JJ cord-304685-s0bfhwtn 8 5 to to IN cord-304685-s0bfhwtn 8 6 DNA dna NN cord-304685-s0bfhwtn 8 7 fragmentation fragmentation NN cord-304685-s0bfhwtn 8 8 , , , cord-304685-s0bfhwtn 8 9 but but CC cord-304685-s0bfhwtn 8 10 not not RB cord-304685-s0bfhwtn 8 11 to to TO cord-304685-s0bfhwtn 8 12 membrane membrane NN cord-304685-s0bfhwtn 8 13 lysis lysis NN cord-304685-s0bfhwtn 8 14 . . . cord-304685-s0bfhwtn 9 1 Log log JJ cord-304685-s0bfhwtn 9 2 phase phase NN cord-304685-s0bfhwtn 9 3 growth growth NN cord-304685-s0bfhwtn 9 4 , , , cord-304685-s0bfhwtn 9 5 transformation transformation NN cord-304685-s0bfhwtn 9 6 with with IN cord-304685-s0bfhwtn 9 7 c c NNP cord-304685-s0bfhwtn 9 8 - - HYPH cord-304685-s0bfhwtn 9 9 myc myc NNP cord-304685-s0bfhwtn 9 10 , , , cord-304685-s0bfhwtn 9 11 or or CC cord-304685-s0bfhwtn 9 12 infection infection NN cord-304685-s0bfhwtn 9 13 of of IN cord-304685-s0bfhwtn 9 14 quiescent quiescent JJ cord-304685-s0bfhwtn 9 15 G0 g0 NN cord-304685-s0bfhwtn 9 16 targets target NNS cord-304685-s0bfhwtn 9 17 with with IN cord-304685-s0bfhwtn 9 18 herpes herpes NN cord-304685-s0bfhwtn 9 19 simplex simplex NNP cord-304685-s0bfhwtn 9 20 virus-1 virus-1 NN cord-304685-s0bfhwtn 9 21 , , , cord-304685-s0bfhwtn 9 22 which which WDT cord-304685-s0bfhwtn 9 23 induces induce VBZ cord-304685-s0bfhwtn 9 24 a a DT cord-304685-s0bfhwtn 9 25 competent competent JJ cord-304685-s0bfhwtn 9 26 state state NN cord-304685-s0bfhwtn 9 27 for for IN cord-304685-s0bfhwtn 9 28 DNA dna NN cord-304685-s0bfhwtn 9 29 synthesis synthesis NN cord-304685-s0bfhwtn 9 30 , , , cord-304685-s0bfhwtn 9 31 all all DT cord-304685-s0bfhwtn 9 32 enhanced enhance VBN cord-304685-s0bfhwtn 9 33 target target NN cord-304685-s0bfhwtn 9 34 cell cell NN cord-304685-s0bfhwtn 9 35 susceptibility susceptibility NN cord-304685-s0bfhwtn 9 36 to to IN cord-304685-s0bfhwtn 9 37 CTL CTL NNP cord-304685-s0bfhwtn 9 38 - - HYPH cord-304685-s0bfhwtn 9 39 induced induce VBN cord-304685-s0bfhwtn 9 40 DNA dna NN cord-304685-s0bfhwtn 9 41 fragmentation fragmentation NN cord-304685-s0bfhwtn 9 42 without without IN cord-304685-s0bfhwtn 9 43 altering alter VBG cord-304685-s0bfhwtn 9 44 the the DT cord-304685-s0bfhwtn 9 45 membrane membrane NN cord-304685-s0bfhwtn 9 46 lysis lysis NN cord-304685-s0bfhwtn 9 47 . . . cord-304685-s0bfhwtn 10 1 These these DT cord-304685-s0bfhwtn 10 2 results result NNS cord-304685-s0bfhwtn 10 3 suggest suggest VBP cord-304685-s0bfhwtn 10 4 that that IN cord-304685-s0bfhwtn 10 5 G0 g0 NN cord-304685-s0bfhwtn 10 6 cells cell NNS cord-304685-s0bfhwtn 10 7 are be VBP cord-304685-s0bfhwtn 10 8 resistant resistant JJ cord-304685-s0bfhwtn 10 9 to to IN cord-304685-s0bfhwtn 10 10 CTL CTL NNP cord-304685-s0bfhwtn 10 11 - - HYPH cord-304685-s0bfhwtn 10 12 induced induce VBN cord-304685-s0bfhwtn 10 13 apoptosis apoptosis NN cord-304685-s0bfhwtn 10 14 , , , cord-304685-s0bfhwtn 10 15 but but CC cord-304685-s0bfhwtn 10 16 that that DT cord-304685-s0bfhwtn 10 17 entry entry NN cord-304685-s0bfhwtn 10 18 into into IN cord-304685-s0bfhwtn 10 19 G1 G1 NNP cord-304685-s0bfhwtn 10 20 or or CC cord-304685-s0bfhwtn 10 21 a a DT cord-304685-s0bfhwtn 10 22 G1-like g1-like CD cord-304685-s0bfhwtn 10 23 state state NN cord-304685-s0bfhwtn 10 24 by by IN cord-304685-s0bfhwtn 10 25 growth growth NN cord-304685-s0bfhwtn 10 26 factors factor NNS cord-304685-s0bfhwtn 10 27 , , , cord-304685-s0bfhwtn 10 28 cellular cellular JJ cord-304685-s0bfhwtn 10 29 transformation transformation NN cord-304685-s0bfhwtn 10 30 , , , cord-304685-s0bfhwtn 10 31 or or CC cord-304685-s0bfhwtn 10 32 DNA dna NN cord-304685-s0bfhwtn 10 33 virus virus NN cord-304685-s0bfhwtn 10 34 infection infection NN cord-304685-s0bfhwtn 10 35 renders render VBZ cord-304685-s0bfhwtn 10 36 them -PRON- PRP cord-304685-s0bfhwtn 10 37 competent competent JJ cord-304685-s0bfhwtn 10 38 to to TO cord-304685-s0bfhwtn 10 39 enter enter VB cord-304685-s0bfhwtn 10 40 the the DT cord-304685-s0bfhwtn 10 41 apoptotic apoptotic JJ cord-304685-s0bfhwtn 10 42 pathway(s pathway(s NN cord-304685-s0bfhwtn 10 43 ) ) -RRB- cord-304685-s0bfhwtn 10 44 . . . cord-304685-s0bfhwtn 11 1 C C NNP cord-304685-s0bfhwtn 11 2 TLs TLs NNP cord-304685-s0bfhwtn 11 3 , , , cord-304685-s0bfhwtn 11 4 which which WDT cord-304685-s0bfhwtn 11 5 are be VBP cord-304685-s0bfhwtn 11 6 principal principal JJ cord-304685-s0bfhwtn 11 7 mediators mediator NNS cord-304685-s0bfhwtn 11 8 in in IN cord-304685-s0bfhwtn 11 9 the the DT cord-304685-s0bfhwtn 11 10 elimination elimination NN cord-304685-s0bfhwtn 11 11 of of IN cord-304685-s0bfhwtn 11 12 transformed transform VBN cord-304685-s0bfhwtn 11 13 and and CC cord-304685-s0bfhwtn 11 14 virus virus NN cord-304685-s0bfhwtn 11 15 - - HYPH cord-304685-s0bfhwtn 11 16 infected infect VBN cord-304685-s0bfhwtn 11 17 cells cell NNS cord-304685-s0bfhwtn 11 18 from from IN cord-304685-s0bfhwtn 11 19 the the DT cord-304685-s0bfhwtn 11 20 host host NN cord-304685-s0bfhwtn 11 21 , , , cord-304685-s0bfhwtn 11 22 kill kill VB cord-304685-s0bfhwtn 11 23 their -PRON- PRP$ cord-304685-s0bfhwtn 11 24 targets target NNS cord-304685-s0bfhwtn 11 25 by by IN cord-304685-s0bfhwtn 11 26 the the DT cord-304685-s0bfhwtn 11 27 direct direct JJ cord-304685-s0bfhwtn 11 28 disruption disruption NN cord-304685-s0bfhwtn 11 29 of of IN cord-304685-s0bfhwtn 11 30 the the DT cord-304685-s0bfhwtn 11 31 plasma plasma NN cord-304685-s0bfhwtn 11 32 membrane membrane NN cord-304685-s0bfhwtn 11 33 and and CC cord-304685-s0bfhwtn 11 34 by by IN cord-304685-s0bfhwtn 11 35 the the DT cord-304685-s0bfhwtn 11 36 induction induction NN cord-304685-s0bfhwtn 11 37 of of IN cord-304685-s0bfhwtn 11 38 the the DT cord-304685-s0bfhwtn 11 39 suicide suicide NN cord-304685-s0bfhwtn 11 40 program program NN cord-304685-s0bfhwtn 11 41 known know VBN cord-304685-s0bfhwtn 11 42 as as IN cord-304685-s0bfhwtn 11 43 apoptosis apoptosis NN cord-304685-s0bfhwtn 11 44 ( ( -LRB- cord-304685-s0bfhwtn 11 45 1 1 CD cord-304685-s0bfhwtn 11 46 ) ) -RRB- cord-304685-s0bfhwtn 11 47 ( ( -LRB- cord-304685-s0bfhwtn 11 48 2 2 LS cord-304685-s0bfhwtn 11 49 ) ) -RRB- cord-304685-s0bfhwtn 11 50 ( ( -LRB- cord-304685-s0bfhwtn 11 51 3 3 LS cord-304685-s0bfhwtn 11 52 ) ) -RRB- cord-304685-s0bfhwtn 11 53 ( ( -LRB- cord-304685-s0bfhwtn 11 54 4 4 LS cord-304685-s0bfhwtn 11 55 ) ) -RRB- cord-304685-s0bfhwtn 11 56 . . . cord-304685-s0bfhwtn 12 1 The the DT cord-304685-s0bfhwtn 12 2 CTEs cte NNS cord-304685-s0bfhwtn 12 3 arsenal arsenal NN cord-304685-s0bfhwtn 12 4 includes include VBZ cord-304685-s0bfhwtn 12 5 the the DT cord-304685-s0bfhwtn 12 6 pore pore NN cord-304685-s0bfhwtn 12 7 - - HYPH cord-304685-s0bfhwtn 12 8 forming form VBG cord-304685-s0bfhwtn 12 9 protein protein NN cord-304685-s0bfhwtn 12 10 perforin perforin NNP cord-304685-s0bfhwtn 12 11 , , , cord-304685-s0bfhwtn 12 12 a a DT cord-304685-s0bfhwtn 12 13 family family NN cord-304685-s0bfhwtn 12 14 ofproteases ofproteases NNP cord-304685-s0bfhwtn 12 15 called call VBN cord-304685-s0bfhwtn 12 16 granzymes granzymes NNP cord-304685-s0bfhwtn 12 17 , , , cord-304685-s0bfhwtn 12 18 and and CC cord-304685-s0bfhwtn 12 19 tumor tumor NN cord-304685-s0bfhwtn 12 20 necrosis necrosis NN cord-304685-s0bfhwtn 12 21 factorlike factorlike NN cord-304685-s0bfhwtn 12 22 molecules molecule NNS cord-304685-s0bfhwtn 12 23 packaged package VBN cord-304685-s0bfhwtn 12 24 together together RB cord-304685-s0bfhwtn 12 25 in in IN cord-304685-s0bfhwtn 12 26 cytoplasmic cytoplasmic JJ cord-304685-s0bfhwtn 12 27 granules granule NNS cord-304685-s0bfhwtn 12 28 ( ( -LRB- cord-304685-s0bfhwtn 12 29 3 3 CD cord-304685-s0bfhwtn 12 30 , , , cord-304685-s0bfhwtn 12 31 4 4 CD cord-304685-s0bfhwtn 12 32 ) ) -RRB- cord-304685-s0bfhwtn 12 33 . . . cord-304685-s0bfhwtn 13 1 The the DT cord-304685-s0bfhwtn 13 2 granule granule NN cord-304685-s0bfhwtn 13 3 exocytosis exocytosis NN cord-304685-s0bfhwtn 13 4 model model NN cord-304685-s0bfhwtn 13 5 of of IN cord-304685-s0bfhwtn 13 6 CTL CTL NNP cord-304685-s0bfhwtn 13 7 killing killing NN cord-304685-s0bfhwtn 13 8 proposes propose VBZ cord-304685-s0bfhwtn 13 9 that that IN cord-304685-s0bfhwtn 13 10 , , , cord-304685-s0bfhwtn 13 11 upon upon IN cord-304685-s0bfhwtn 13 12 recognition recognition NN cord-304685-s0bfhwtn 13 13 of of IN cord-304685-s0bfhwtn 13 14 viral viral JJ cord-304685-s0bfhwtn 13 15 - - HYPH cord-304685-s0bfhwtn 13 16 or or CC cord-304685-s0bfhwtn 13 17 tumor tumor NN cord-304685-s0bfhwtn 13 18 - - HYPH cord-304685-s0bfhwtn 13 19 associated associate VBN cord-304685-s0bfhwtn 13 20 antigen(s antigen(s NNP cord-304685-s0bfhwtn 13 21 ) ) -RRB- cord-304685-s0bfhwtn 13 22 presented present VBD cord-304685-s0bfhwtn 13 23 via via IN cord-304685-s0bfhwtn 13 24 the the DT cord-304685-s0bfhwtn 13 25 MHC MHC NNP cord-304685-s0bfhwtn 13 26 class class NN cord-304685-s0bfhwtn 13 27 I -PRON- PRP cord-304685-s0bfhwtn 13 28 mdecule mdecule VBP cord-304685-s0bfhwtn 13 29 , , , cord-304685-s0bfhwtn 13 30 the the DT cord-304685-s0bfhwtn 13 31 CTL CTL NNP cord-304685-s0bfhwtn 13 32 is be VBZ cord-304685-s0bfhwtn 13 33 triggered trigger VBN cord-304685-s0bfhwtn 13 34 to to TO cord-304685-s0bfhwtn 13 35 release release VB cord-304685-s0bfhwtn 13 36 its -PRON- PRP$ cord-304685-s0bfhwtn 13 37 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 13 38 granule granule NN cord-304685-s0bfhwtn 13 39 components component NNS cord-304685-s0bfhwtn 13 40 onto onto IN cord-304685-s0bfhwtn 13 41 the the DT cord-304685-s0bfhwtn 13 42 target target NN cord-304685-s0bfhwtn 13 43 cell cell NN cord-304685-s0bfhwtn 13 44 's 's POS cord-304685-s0bfhwtn 13 45 plasma plasma NN cord-304685-s0bfhwtn 13 46 membrane membrane NN cord-304685-s0bfhwtn 13 47 . . . cord-304685-s0bfhwtn 14 1 Perforin Perforin NNP cord-304685-s0bfhwtn 14 2 inserts insert NNS cord-304685-s0bfhwtn 14 3 into into IN cord-304685-s0bfhwtn 14 4 the the DT cord-304685-s0bfhwtn 14 5 target target NN cord-304685-s0bfhwtn 14 6 's 's POS cord-304685-s0bfhwtn 14 7 plasma plasma NN cord-304685-s0bfhwtn 14 8 membrane membrane NN cord-304685-s0bfhwtn 14 9 and and CC cord-304685-s0bfhwtn 14 10 forms form VBZ cord-304685-s0bfhwtn 14 11 transmembrane transmembrane NN cord-304685-s0bfhwtn 14 12 channds channd NNS cord-304685-s0bfhwtn 14 13 to to TO cord-304685-s0bfhwtn 14 14 cause cause VB cord-304685-s0bfhwtn 14 15 the the DT cord-304685-s0bfhwtn 14 16 osmotic osmotic JJ cord-304685-s0bfhwtn 14 17 lysis lysis NN cord-304685-s0bfhwtn 14 18 of of IN cord-304685-s0bfhwtn 14 19 the the DT cord-304685-s0bfhwtn 14 20 target target NN cord-304685-s0bfhwtn 14 21 ( ( -LRB- cord-304685-s0bfhwtn 14 22 3 3 CD cord-304685-s0bfhwtn 14 23 ) ) -RRB- cord-304685-s0bfhwtn 14 24 . . . cord-304685-s0bfhwtn 15 1 The the DT cord-304685-s0bfhwtn 15 2 remaining remain VBG cord-304685-s0bfhwtn 15 3 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 15 4 granule granule NN cord-304685-s0bfhwtn 15 5 components component NNS cord-304685-s0bfhwtn 15 6 interact interact VBP cord-304685-s0bfhwtn 15 7 with with IN cord-304685-s0bfhwtn 15 8 as as RB cord-304685-s0bfhwtn 15 9 yet yet RB cord-304685-s0bfhwtn 15 10 undefined undefined JJ cord-304685-s0bfhwtn 15 11 target target NN cord-304685-s0bfhwtn 15 12 cell cell NN cord-304685-s0bfhwtn 15 13 dements dement NNS cord-304685-s0bfhwtn 15 14 to to TO cord-304685-s0bfhwtn 15 15 trigger trigger VB cord-304685-s0bfhwtn 15 16 the the DT cord-304685-s0bfhwtn 15 17 apoptotic apoptotic JJ cord-304685-s0bfhwtn 15 18 death death NN cord-304685-s0bfhwtn 15 19 of of IN cord-304685-s0bfhwtn 15 20 the the DT cord-304685-s0bfhwtn 15 21 target target NN cord-304685-s0bfhwtn 15 22 , , , cord-304685-s0bfhwtn 15 23 characterized characterize VBN cord-304685-s0bfhwtn 15 24 by by IN cord-304685-s0bfhwtn 15 25 cellular cellular JJ cord-304685-s0bfhwtn 15 26 disintegration disintegration NN cord-304685-s0bfhwtn 15 27 and and CC cord-304685-s0bfhwtn 15 28 fragmentation fragmentation NN cord-304685-s0bfhwtn 15 29 of of IN cord-304685-s0bfhwtn 15 30 the the DT cord-304685-s0bfhwtn 15 31 target target NN cord-304685-s0bfhwtn 15 32 's 's POS cord-304685-s0bfhwtn 15 33 DNA dna NN cord-304685-s0bfhwtn 15 34 into into IN cord-304685-s0bfhwtn 15 35 nudeosome nudeosome NN cord-304685-s0bfhwtn 15 36 particles particle NNS cord-304685-s0bfhwtn 15 37 ( ( -LRB- cord-304685-s0bfhwtn 15 38 2 2 CD cord-304685-s0bfhwtn 15 39 ) ) -RRB- cord-304685-s0bfhwtn 15 40 ( ( -LRB- cord-304685-s0bfhwtn 15 41 3 3 LS cord-304685-s0bfhwtn 15 42 ) ) -RRB- cord-304685-s0bfhwtn 15 43 ( ( -LRB- cord-304685-s0bfhwtn 15 44 4 4 LS cord-304685-s0bfhwtn 15 45 ) ) -RRB- cord-304685-s0bfhwtn 15 46 . . . cord-304685-s0bfhwtn 16 1 In in IN cord-304685-s0bfhwtn 16 2 a a DT cord-304685-s0bfhwtn 16 3 previous previous JJ cord-304685-s0bfhwtn 16 4 investigation investigation NN cord-304685-s0bfhwtn 16 5 ( ( -LRB- cord-304685-s0bfhwtn 16 6 5 5 LS cord-304685-s0bfhwtn 16 7 ) ) -RRB- cord-304685-s0bfhwtn 16 8 , , , cord-304685-s0bfhwtn 16 9 we -PRON- PRP cord-304685-s0bfhwtn 16 10 demonstrated demonstrate VBD cord-304685-s0bfhwtn 16 11 that that IN cord-304685-s0bfhwtn 16 12 specific specific JJ cord-304685-s0bfhwtn 16 13 inhibitors inhibitor NNS cord-304685-s0bfhwtn 16 14 for for IN cord-304685-s0bfhwtn 16 15 DNA dna NN cord-304685-s0bfhwtn 16 16 topoisomerases topoisomerase NNS cord-304685-s0bfhwtn 16 17 I -PRON- PRP cord-304685-s0bfhwtn 16 18 ( ( -LRB- cord-304685-s0bfhwtn 16 19 camptothecin camptothecin NNP cord-304685-s0bfhwtn 16 20 ) ) -RRB- cord-304685-s0bfhwtn 16 21 and and CC cord-304685-s0bfhwtn 16 22 II II NNP cord-304685-s0bfhwtn 16 23 ( ( -LRB- cord-304685-s0bfhwtn 16 24 mAMSA mAMSA NNP cord-304685-s0bfhwtn 16 25 and and CC cord-304685-s0bfhwtn 16 26 VM-26 VM-26 NNP cord-304685-s0bfhwtn 16 27 ) ) -RRB- cord-304685-s0bfhwtn 16 28 prevent prevent VB cord-304685-s0bfhwtn 16 29 the the DT cord-304685-s0bfhwtn 16 30 CTL CTL NNP cord-304685-s0bfhwtn 16 31 - - HYPH cord-304685-s0bfhwtn 16 32 induced induce VBN cord-304685-s0bfhwtn 16 33 DNA dna NN cord-304685-s0bfhwtn 16 34 fragmentation fragmentation NN cord-304685-s0bfhwtn 16 35 of of IN cord-304685-s0bfhwtn 16 36 the the DT cord-304685-s0bfhwtn 16 37 target target NN cord-304685-s0bfhwtn 16 38 cell cell NN cord-304685-s0bfhwtn 16 39 's 's POS cord-304685-s0bfhwtn 16 40 DNA dna NN cord-304685-s0bfhwtn 16 41 without without IN cord-304685-s0bfhwtn 16 42 impairing impair VBG cord-304685-s0bfhwtn 16 43 the the DT cord-304685-s0bfhwtn 16 44 disruption disruption NN cord-304685-s0bfhwtn 16 45 of of IN cord-304685-s0bfhwtn 16 46 the the DT cord-304685-s0bfhwtn 16 47 target target NN cord-304685-s0bfhwtn 16 48 cell cell NN cord-304685-s0bfhwtn 16 49 's 's POS cord-304685-s0bfhwtn 16 50 plasma plasma NN cord-304685-s0bfhwtn 16 51 membrane membrane NN cord-304685-s0bfhwtn 16 52 . . . cord-304685-s0bfhwtn 17 1 DNA DNA NNP cord-304685-s0bfhwtn 17 2 topoisomerases topoisomerase NNS cord-304685-s0bfhwtn 17 3 are be VBP cord-304685-s0bfhwtn 17 4 cdl cdl NNP cord-304685-s0bfhwtn 17 5 cycle cycle NN cord-304685-s0bfhwtn 17 6 - - HYPH cord-304685-s0bfhwtn 17 7 regulated regulate VBN cord-304685-s0bfhwtn 17 8 enzymes enzyme NNS cord-304685-s0bfhwtn 17 9 with with IN cord-304685-s0bfhwtn 17 10 low low JJ cord-304685-s0bfhwtn 17 11 to to IN cord-304685-s0bfhwtn 17 12 undetectable undetectable JJ cord-304685-s0bfhwtn 17 13 activity activity NN cord-304685-s0bfhwtn 17 14 in in IN cord-304685-s0bfhwtn 17 15 early early JJ cord-304685-s0bfhwtn 17 16 G1 g1 NN cord-304685-s0bfhwtn 17 17 and and CC cord-304685-s0bfhwtn 17 18 peak peak NN cord-304685-s0bfhwtn 17 19 activity activity NN cord-304685-s0bfhwtn 17 20 in in IN cord-304685-s0bfhwtn 17 21 the the DT cord-304685-s0bfhwtn 17 22 S S NNP cord-304685-s0bfhwtn 17 23 and and CC cord-304685-s0bfhwtn 17 24 GO GO NNP cord-304685-s0bfhwtn 17 25 / / SYM cord-304685-s0bfhwtn 17 26 M M NNP cord-304685-s0bfhwtn 17 27 phases phase NNS cord-304685-s0bfhwtn 17 28 of of IN cord-304685-s0bfhwtn 17 29 the the DT cord-304685-s0bfhwtn 17 30 cell cell NN cord-304685-s0bfhwtn 17 31 cycle cycle NN cord-304685-s0bfhwtn 17 32 ( ( -LRB- cord-304685-s0bfhwtn 17 33 6 6 CD cord-304685-s0bfhwtn 17 34 ) ) -RRB- cord-304685-s0bfhwtn 17 35 . . . cord-304685-s0bfhwtn 18 1 We -PRON- PRP cord-304685-s0bfhwtn 18 2 therefore therefore RB cord-304685-s0bfhwtn 18 3 , , , cord-304685-s0bfhwtn 18 4 sought seek VBD cord-304685-s0bfhwtn 18 5 to to TO cord-304685-s0bfhwtn 18 6 determine determine VB cord-304685-s0bfhwtn 18 7 whether whether IN cord-304685-s0bfhwtn 18 8 the the DT cord-304685-s0bfhwtn 18 9 susceptibility susceptibility NN cord-304685-s0bfhwtn 18 10 of of IN cord-304685-s0bfhwtn 18 11 target target NN cord-304685-s0bfhwtn 18 12 cells cell NNS cord-304685-s0bfhwtn 18 13 to to IN cord-304685-s0bfhwtn 18 14 CTLinduced ctlinduce VBN cord-304685-s0bfhwtn 18 15 apoptosis apoptosis NN cord-304685-s0bfhwtn 18 16 was be VBD cord-304685-s0bfhwtn 18 17 cdl cdl NNP cord-304685-s0bfhwtn 18 18 cycle cycle NN cord-304685-s0bfhwtn 18 19 - - HYPH cord-304685-s0bfhwtn 18 20 related relate VBN cord-304685-s0bfhwtn 18 21 and and CC cord-304685-s0bfhwtn 18 22 show show VBP cord-304685-s0bfhwtn 18 23 here here RB cord-304685-s0bfhwtn 18 24 that that IN cord-304685-s0bfhwtn 18 25 it -PRON- PRP cord-304685-s0bfhwtn 18 26 is be VBZ cord-304685-s0bfhwtn 18 27 . . . cord-304685-s0bfhwtn 19 1 Effector Effector NNP cord-304685-s0bfhwtn 19 2 Cells cell NNS cord-304685-s0bfhwtn 19 3 . . . cord-304685-s0bfhwtn 20 1 In in IN cord-304685-s0bfhwtn 20 2 this this DT cord-304685-s0bfhwtn 20 3 investigation investigation NN cord-304685-s0bfhwtn 20 4 we -PRON- PRP cord-304685-s0bfhwtn 20 5 used use VBD cord-304685-s0bfhwtn 20 6 purified purify VBN cord-304685-s0bfhwtn 20 7 primary primary JJ cord-304685-s0bfhwtn 20 8 CD8 CD8 NNP cord-304685-s0bfhwtn 20 9 + + CC cord-304685-s0bfhwtn 20 10 spleen spleen NNP cord-304685-s0bfhwtn 20 11 CTLs ctl NNS cord-304685-s0bfhwtn 20 12 , , , cord-304685-s0bfhwtn 20 13 induced induce VBN cord-304685-s0bfhwtn 20 14 by by IN cord-304685-s0bfhwtn 20 15 acute acute JJ cord-304685-s0bfhwtn 20 16 infection infection NN cord-304685-s0bfhwtn 20 17 of of IN cord-304685-s0bfhwtn 20 18 adult adult NN cord-304685-s0bfhwtn 20 19 C3H/ C3H/ NNP cord-304685-s0bfhwtn 20 20 HeSnJ HeSnJ NNP cord-304685-s0bfhwtn 20 21 x x NNP cord-304685-s0bfhwtn 20 22 C57BL/6J)F1 c57bl/6j)f1 NN cord-304685-s0bfhwtn 20 23 mice mouse NNS cord-304685-s0bfhwtn 20 24 for for IN cord-304685-s0bfhwtn 20 25 8 8 CD cord-304685-s0bfhwtn 20 26 d d NN cord-304685-s0bfhwtn 20 27 with with IN cord-304685-s0bfhwtn 20 28 lymphocytic lymphocytic JJ cord-304685-s0bfhwtn 20 29 choriomeningitis choriomeningitis NNP cord-304685-s0bfhwtn 20 30 virus virus NN cord-304685-s0bfhwtn 20 31 ( ( -LRB- cord-304685-s0bfhwtn 20 32 LCMV LCMV NNP cord-304685-s0bfhwtn 20 33 ) ) -RRB- cord-304685-s0bfhwtn 20 34 , , , cord-304685-s0bfhwtn 20 35 strain strain VBP cord-304685-s0bfhwtn 20 36 Armstrong Armstrong NNP cord-304685-s0bfhwtn 20 37 ( ( -LRB- cord-304685-s0bfhwtn 20 38 5 5 CD cord-304685-s0bfhwtn 20 39 ) ) -RRB- cord-304685-s0bfhwtn 20 40 . . . cord-304685-s0bfhwtn 21 1 The the DT cord-304685-s0bfhwtn 21 2 use use NN cord-304685-s0bfhwtn 21 3 of of IN cord-304685-s0bfhwtn 21 4 these these DT cord-304685-s0bfhwtn 21 5 primary primary JJ cord-304685-s0bfhwtn 21 6 CTL CTL NNP cord-304685-s0bfhwtn 21 7 ( ( -LRB- cord-304685-s0bfhwtn 21 8 LCMV LCMV NNP cord-304685-s0bfhwtn 21 9 - - HYPH cord-304685-s0bfhwtn 21 10 CTL CTL NNP cord-304685-s0bfhwtn 21 11 ) ) -RRB- cord-304685-s0bfhwtn 21 12 precludes preclude VBZ cord-304685-s0bfhwtn 21 13 artifactual artifactual JJ cord-304685-s0bfhwtn 21 14 problems problem NNS cord-304685-s0bfhwtn 21 15 associated associate VBN cord-304685-s0bfhwtn 21 16 with with IN cord-304685-s0bfhwtn 21 17 CTL CTL NNP cord-304685-s0bfhwtn 21 18 lines line NNS cord-304685-s0bfhwtn 21 19 . . . cord-304685-s0bfhwtn 22 1 These these DT cord-304685-s0bfhwtn 22 2 LCMV LCMV NNP cord-304685-s0bfhwtn 22 3 - - HYPH cord-304685-s0bfhwtn 22 4 CTL CTL NNP cord-304685-s0bfhwtn 22 5 contain contain VBP cord-304685-s0bfhwtn 22 6 azurophilic azurophilic JJ cord-304685-s0bfhwtn 22 7 granules granule NNS cord-304685-s0bfhwtn 22 8 and and CC cord-304685-s0bfhwtn 22 9 specifically specifically RB cord-304685-s0bfhwtn 22 10 induce induce VB cord-304685-s0bfhwtn 22 11 the the DT cord-304685-s0bfhwtn 22 12 lysis lysis NN cord-304685-s0bfhwtn 22 13 and and CC cord-304685-s0bfhwtn 22 14 DNA dna NN cord-304685-s0bfhwtn 22 15 fragraentation fragraentation NN cord-304685-s0bfhwtn 22 16 of of IN cord-304685-s0bfhwtn 22 17 LCMV LCMV NNP cord-304685-s0bfhwtn 22 18 - - HYPH cord-304685-s0bfhwtn 22 19 infected infect VBN cord-304685-s0bfhwtn 22 20 targets target NNS cord-304685-s0bfhwtn 22 21 in in IN cord-304685-s0bfhwtn 22 22 an an DT cord-304685-s0bfhwtn 22 23 MHC MHC NNP cord-304685-s0bfhwtn 22 24 class class NN cord-304685-s0bfhwtn 22 25 I i NN cord-304685-s0bfhwtn 22 26 - - HYPH cord-304685-s0bfhwtn 22 27 restricted restrict VBN cord-304685-s0bfhwtn 22 28 fashion fashion NN cord-304685-s0bfhwtn 22 29 ( ( -LRB- cord-304685-s0bfhwtn 22 30 7 7 CD cord-304685-s0bfhwtn 22 31 ) ) -RRB- cord-304685-s0bfhwtn 22 32 . . . cord-304685-s0bfhwtn 23 1 A a DT cord-304685-s0bfhwtn 23 2 Con Con NNP cord-304685-s0bfhwtn 23 3 A a DT cord-304685-s0bfhwtn 23 4 lectin lectin NN cord-304685-s0bfhwtn 23 5 - - HYPH cord-304685-s0bfhwtn 23 6 dependent dependent JJ cord-304685-s0bfhwtn 23 7 killing killing NN cord-304685-s0bfhwtn 23 8 assay assay NN cord-304685-s0bfhwtn 23 9 was be VBD cord-304685-s0bfhwtn 23 10 used use VBN cord-304685-s0bfhwtn 23 11 to to TO cord-304685-s0bfhwtn 23 12 bypass bypass VB cord-304685-s0bfhwtn 23 13 the the DT cord-304685-s0bfhwtn 23 14 requirements requirement NNS cord-304685-s0bfhwtn 23 15 for for IN cord-304685-s0bfhwtn 23 16 specific specific JJ cord-304685-s0bfhwtn 23 17 antigen antigen NN cord-304685-s0bfhwtn 23 18 , , , cord-304685-s0bfhwtn 23 19 in in IN cord-304685-s0bfhwtn 23 20 order order NN cord-304685-s0bfhwtn 23 21 to to TO cord-304685-s0bfhwtn 23 22 compare compare VB cord-304685-s0bfhwtn 23 23 the the DT cord-304685-s0bfhwtn 23 24 susceptibility susceptibility NN cord-304685-s0bfhwtn 23 25 of of IN cord-304685-s0bfhwtn 23 26 various various JJ cord-304685-s0bfhwtn 23 27 target target NN cord-304685-s0bfhwtn 23 28 cells cell NNS cord-304685-s0bfhwtn 23 29 to to IN cord-304685-s0bfhwtn 23 30 CTL CTL NNP cord-304685-s0bfhwtn 23 31 - - HYPH cord-304685-s0bfhwtn 23 32 induced induce VBN cord-304685-s0bfhwtn 23 33 apoptosis apoptosis NN cord-304685-s0bfhwtn 23 34 ( ( -LRB- cord-304685-s0bfhwtn 23 35 5 5 CD cord-304685-s0bfhwtn 23 36 ) ) -RRB- cord-304685-s0bfhwtn 23 37 . . . cord-304685-s0bfhwtn 24 1 CTL CTL NNP cord-304685-s0bfhwtn 24 2 Assa?s assa?s RB cord-304685-s0bfhwtn 24 3 . . . cord-304685-s0bfhwtn 25 1 Mouse Mouse NNP cord-304685-s0bfhwtn 25 2 target target NN cord-304685-s0bfhwtn 25 3 ceils ceil NNS cord-304685-s0bfhwtn 25 4 ( ( -LRB- cord-304685-s0bfhwtn 25 5 L-929 l-929 NN cord-304685-s0bfhwtn 25 6 , , . cord-304685-s0bfhwtn 26 1 BALB BALB NNP cord-304685-s0bfhwtn 26 2 / / SYM cord-304685-s0bfhwtn 26 3 c c NN cord-304685-s0bfhwtn 26 4 NIH NIH NNP cord-304685-s0bfhwtn 26 5 3T3 3t3 CD cord-304685-s0bfhwtn 26 6 , , , cord-304685-s0bfhwtn 26 7 P815 P815 '' cord-304685-s0bfhwtn 26 8 , , , cord-304685-s0bfhwtn 26 9 YAC-1 YAC-1 NNP cord-304685-s0bfhwtn 26 10 , , , cord-304685-s0bfhwtn 26 11 and and CC cord-304685-s0bfhwtn 26 12 A20 A20 NNP cord-304685-s0bfhwtn 26 13 ) ) -RRB- cord-304685-s0bfhwtn 26 14 were be VBD cord-304685-s0bfhwtn 26 15 labded labde VBN cord-304685-s0bfhwtn 26 16 either either CC cord-304685-s0bfhwtn 26 17 with with IN cord-304685-s0bfhwtn 26 18 sodium sodium NN cord-304685-s0bfhwtn 26 19 Slchromate Slchromate NNP cord-304685-s0bfhwtn 26 20 for for IN cord-304685-s0bfhwtn 26 21 a a DT cord-304685-s0bfhwtn 26 22 cytoplasmic cytoplasmic JJ cord-304685-s0bfhwtn 26 23 label label NN cord-304685-s0bfhwtn 26 24 or or CC cord-304685-s0bfhwtn 26 25 with with IN cord-304685-s0bfhwtn 26 26 [ [ -LRB- cord-304685-s0bfhwtn 26 27 SH]thymidine SH]thymidine NNP cord-304685-s0bfhwtn 26 28 or or CC cord-304685-s0bfhwtn 26 29 [ [ -LRB- cord-304685-s0bfhwtn 26 30 mI]iododeoxyuridine mI]iododeoxyuridine NFP cord-304685-s0bfhwtn 26 31 ( ( -LRB- cord-304685-s0bfhwtn 26 32 12sI 12si CD cord-304685-s0bfhwtn 26 33 - - HYPH cord-304685-s0bfhwtn 26 34 IUDR IUDR NNP cord-304685-s0bfhwtn 26 35 . . . cord-304685-s0bfhwtn 26 36 ) ) -RRB- cord-304685-s0bfhwtn 26 37 for for IN cord-304685-s0bfhwtn 26 38 a a DT cord-304685-s0bfhwtn 26 39 DNA dna NN cord-304685-s0bfhwtn 26 40 label label NN cord-304685-s0bfhwtn 26 41 and and CC cord-304685-s0bfhwtn 26 42 examined examine VBD cord-304685-s0bfhwtn 26 43 for for IN cord-304685-s0bfhwtn 26 44 the the DT cord-304685-s0bfhwtn 26 45 release release NN cord-304685-s0bfhwtn 26 46 of of IN cord-304685-s0bfhwtn 26 47 labels label NNS cord-304685-s0bfhwtn 26 48 as as IN cord-304685-s0bfhwtn 26 49 specified specify VBN cord-304685-s0bfhwtn 26 50 in in IN cord-304685-s0bfhwtn 26 51 the the DT cord-304685-s0bfhwtn 26 52 figure figure NN cord-304685-s0bfhwtn 26 53 legends legend NNS cord-304685-s0bfhwtn 26 54 . . . cord-304685-s0bfhwtn 27 1 Where where WRB cord-304685-s0bfhwtn 27 2 indicated indicate VBN cord-304685-s0bfhwtn 27 3 , , , cord-304685-s0bfhwtn 27 4 DNA DNA NNP cord-304685-s0bfhwtn 27 5 was be VBD cord-304685-s0bfhwtn 27 6 purified purify VBN cord-304685-s0bfhwtn 27 7 by by IN cord-304685-s0bfhwtn 27 8 guanidine guanidine NNP cord-304685-s0bfhwtn 27 9 HC1 HC1 NNP cord-304685-s0bfhwtn 27 10 denaturation denaturation NN cord-304685-s0bfhwtn 27 11 in in IN cord-304685-s0bfhwtn 27 12 the the DT cord-304685-s0bfhwtn 27 13 absence absence NN cord-304685-s0bfhwtn 27 14 of of IN cord-304685-s0bfhwtn 27 15 detergent detergent NN cord-304685-s0bfhwtn 27 16 and and CC cord-304685-s0bfhwtn 27 17 dectrophoresed dectrophorese VBD cord-304685-s0bfhwtn 27 18 in in IN cord-304685-s0bfhwtn 27 19 1.5 1.5 CD cord-304685-s0bfhwtn 27 20 % % NN cord-304685-s0bfhwtn 27 21 agarose agarose JJ cord-304685-s0bfhwtn 27 22 gels gel NNS cord-304685-s0bfhwtn 27 23 , , , cord-304685-s0bfhwtn 27 24 as as IN cord-304685-s0bfhwtn 27 25 described describe VBN cord-304685-s0bfhwtn 27 26 ( ( -LRB- cord-304685-s0bfhwtn 27 27 5 5 CD cord-304685-s0bfhwtn 27 28 ) ) -RRB- cord-304685-s0bfhwtn 27 29 . . . cord-304685-s0bfhwtn 28 1 When when WRB cord-304685-s0bfhwtn 28 2 fibroblasts fibroblast NNS cord-304685-s0bfhwtn 28 3 are be VBP cord-304685-s0bfhwtn 28 4 cultured culture VBN cord-304685-s0bfhwtn 28 5 in in IN cord-304685-s0bfhwtn 28 6 decreasing decrease VBG cord-304685-s0bfhwtn 28 7 concentrations concentration NNS cord-304685-s0bfhwtn 28 8 of of IN cord-304685-s0bfhwtn 28 9 serum serum NN cord-304685-s0bfhwtn 28 10 , , , cord-304685-s0bfhwtn 28 11 there there EX cord-304685-s0bfhwtn 28 12 is be VBZ cord-304685-s0bfhwtn 28 13 a a DT cord-304685-s0bfhwtn 28 14 concomitant concomitant JJ cord-304685-s0bfhwtn 28 15 decrease decrease NN cord-304685-s0bfhwtn 28 16 in in IN cord-304685-s0bfhwtn 28 17 the the DT cord-304685-s0bfhwtn 28 18 percentage percentage NN cord-304685-s0bfhwtn 28 19 of of IN cord-304685-s0bfhwtn 28 20 cells cell NNS cord-304685-s0bfhwtn 28 21 in in IN cord-304685-s0bfhwtn 28 22 log log NN cord-304685-s0bfhwtn 28 23 phase phase NN cord-304685-s0bfhwtn 28 24 growth growth NN cord-304685-s0bfhwtn 28 25 ( ( -LRB- cord-304685-s0bfhwtn 28 26 8) 8) NN cord-304685-s0bfhwtn 28 27 . . . cord-304685-s0bfhwtn 29 1 Culture culture NN cord-304685-s0bfhwtn 29 2 of of IN cord-304685-s0bfhwtn 29 3 L929 l929 JJ cord-304685-s0bfhwtn 29 4 fibroblasts fibroblast NNS cord-304685-s0bfhwtn 29 5 in in IN cord-304685-s0bfhwtn 29 6 2 2 CD cord-304685-s0bfhwtn 29 7 , , , cord-304685-s0bfhwtn 29 8 5 5 CD cord-304685-s0bfhwtn 29 9 , , , cord-304685-s0bfhwtn 29 10 10 10 CD cord-304685-s0bfhwtn 29 11 , , , cord-304685-s0bfhwtn 29 12 or or CC cord-304685-s0bfhwtn 29 13 20 20 CD cord-304685-s0bfhwtn 29 14 % % NN cord-304685-s0bfhwtn 29 15 FBS FBS NNP cord-304685-s0bfhwtn 29 16 led lead VBD cord-304685-s0bfhwtn 29 17 to to IN cord-304685-s0bfhwtn 29 18 greater great JJR cord-304685-s0bfhwtn 29 19 cell cell NN cord-304685-s0bfhwtn 29 20 proliferation proliferation NN cord-304685-s0bfhwtn 29 21 with with IN cord-304685-s0bfhwtn 29 22 increasing increase VBG cord-304685-s0bfhwtn 29 23 FBS FBS NNP cord-304685-s0bfhwtn 29 24 concentration concentration NN cord-304685-s0bfhwtn 29 25 . . . cord-304685-s0bfhwtn 30 1 After after IN cord-304685-s0bfhwtn 30 2 2 2 CD cord-304685-s0bfhwtn 30 3 d d NN cord-304685-s0bfhwtn 30 4 of of IN cord-304685-s0bfhwtn 30 5 growth growth NN cord-304685-s0bfhwtn 30 6 under under IN cord-304685-s0bfhwtn 30 7 these these DT cord-304685-s0bfhwtn 30 8 conditions condition NNS cord-304685-s0bfhwtn 30 9 , , , cord-304685-s0bfhwtn 30 10 [ [ -LRB- cord-304685-s0bfhwtn 30 11 ~H]thymidine ~h]thymidine JJ cord-304685-s0bfhwtn 30 12 uptake uptake NN cord-304685-s0bfhwtn 30 13 was be VBD cord-304685-s0bfhwtn 30 14 12 12 CD cord-304685-s0bfhwtn 30 15 , , , cord-304685-s0bfhwtn 30 16 29 29 CD cord-304685-s0bfhwtn 30 17 , , , cord-304685-s0bfhwtn 30 18 45 45 CD cord-304685-s0bfhwtn 30 19 , , , cord-304685-s0bfhwtn 30 20 and and CC cord-304685-s0bfhwtn 31 1 87 87 CD cord-304685-s0bfhwtn 31 2 x x SYM cord-304685-s0bfhwtn 31 3 103 103 CD cord-304685-s0bfhwtn 31 4 cpm cpm NN cord-304685-s0bfhwtn 31 5 , , , cord-304685-s0bfhwtn 31 6 respectively respectively RB cord-304685-s0bfhwtn 31 7 , , , cord-304685-s0bfhwtn 31 8 after after IN cord-304685-s0bfhwtn 31 9 a a DT cord-304685-s0bfhwtn 31 10 1-h 1-h CD cord-304685-s0bfhwtn 31 11 pulse pulse NN cord-304685-s0bfhwtn 31 12 . . . cord-304685-s0bfhwtn 32 1 When when WRB cord-304685-s0bfhwtn 32 2 assayed assay VBN cord-304685-s0bfhwtn 32 3 as as IN cord-304685-s0bfhwtn 32 4 CTL CTL NNP cord-304685-s0bfhwtn 32 5 targets target NNS cord-304685-s0bfhwtn 32 6 in in IN cord-304685-s0bfhwtn 32 7 their -PRON- PRP$ cord-304685-s0bfhwtn 32 8 respective respective JJ cord-304685-s0bfhwtn 32 9 culture culture NN cord-304685-s0bfhwtn 32 10 conditions condition NNS cord-304685-s0bfhwtn 32 11 , , , cord-304685-s0bfhwtn 32 12 there there EX cord-304685-s0bfhwtn 32 13 was be VBD cord-304685-s0bfhwtn 32 14 no no DT cord-304685-s0bfhwtn 32 15 significant significant JJ cord-304685-s0bfhwtn 32 16 difference difference NN cord-304685-s0bfhwtn 32 17 in in IN cord-304685-s0bfhwtn 32 18 the the DT cord-304685-s0bfhwtn 32 19 membrane membrane NN cord-304685-s0bfhwtn 32 20 lysis lysis NN cord-304685-s0bfhwtn 32 21 of of IN cord-304685-s0bfhwtn 32 22 these these DT cord-304685-s0bfhwtn 32 23 cells cell NNS cord-304685-s0bfhwtn 32 24 as as IN cord-304685-s0bfhwtn 32 25 assessed assess VBN cord-304685-s0bfhwtn 32 26 by by IN cord-304685-s0bfhwtn 32 27 release release NN cord-304685-s0bfhwtn 32 28 of of IN cord-304685-s0bfhwtn 32 29 the the DT cord-304685-s0bfhwtn 32 30 cytoplasmic cytoplasmic JJ cord-304685-s0bfhwtn 32 31 radiolabel radiolabel NN cord-304685-s0bfhwtn 32 32 , , , cord-304685-s0bfhwtn 32 33 5tCr 5tcr CD cord-304685-s0bfhwtn 32 34 ( ( -LRB- cord-304685-s0bfhwtn 32 35 Fig Fig NNP cord-304685-s0bfhwtn 32 36 . . . cord-304685-s0bfhwtn 32 37 _SP cord-304685-s0bfhwtn 33 1 1 1 LS cord-304685-s0bfhwtn 33 2 A a CD cord-304685-s0bfhwtn 33 3 ) ) -RRB- cord-304685-s0bfhwtn 33 4 . . . cord-304685-s0bfhwtn 34 1 However however RB cord-304685-s0bfhwtn 34 2 , , , cord-304685-s0bfhwtn 34 3 the the DT cord-304685-s0bfhwtn 34 4 fragmentation fragmentation NN cord-304685-s0bfhwtn 34 5 of of IN cord-304685-s0bfhwtn 34 6 the the DT cord-304685-s0bfhwtn 34 7 target target NN cord-304685-s0bfhwtn 34 8 DNA dna NN cord-304685-s0bfhwtn 34 9 , , , cord-304685-s0bfhwtn 34 10 as as IN cord-304685-s0bfhwtn 34 11 assessed assess VBN cord-304685-s0bfhwtn 34 12 by by IN cord-304685-s0bfhwtn 34 13 liberation liberation NN cord-304685-s0bfhwtn 34 14 of of IN cord-304685-s0bfhwtn 35 1 a a DT cord-304685-s0bfhwtn 35 2 125I 125I NNP cord-304685-s0bfhwtn 35 3 - - HYPH cord-304685-s0bfhwtn 35 4 IUDR IUDR NNP cord-304685-s0bfhwtn 35 5 . . . cord-304685-s0bfhwtn 35 6 label label NNP cord-304685-s0bfhwtn 35 7 , , , cord-304685-s0bfhwtn 35 8 increased increase VBD cord-304685-s0bfhwtn 35 9 dramatically dramatically RB cord-304685-s0bfhwtn 35 10 in in IN cord-304685-s0bfhwtn 35 11 cells cell NNS cord-304685-s0bfhwtn 35 12 grown grow VBN cord-304685-s0bfhwtn 35 13 in in IN cord-304685-s0bfhwtn 35 14 increasing increase VBG cord-304685-s0bfhwtn 35 15 serum serum NN cord-304685-s0bfhwtn 35 16 concentrations concentration NNS cord-304685-s0bfhwtn 35 17 ( ( -LRB- cord-304685-s0bfhwtn 35 18 Fig fig NN cord-304685-s0bfhwtn 35 19 . . . cord-304685-s0bfhwtn 35 20 1 1 CD cord-304685-s0bfhwtn 35 21 B b NN cord-304685-s0bfhwtn 35 22 ) ) -RRB- cord-304685-s0bfhwtn 35 23 . . . cord-304685-s0bfhwtn 36 1 These these DT cord-304685-s0bfhwtn 36 2 results result NNS cord-304685-s0bfhwtn 36 3 suggested suggest VBD cord-304685-s0bfhwtn 36 4 that that IN cord-304685-s0bfhwtn 36 5 the the DT cord-304685-s0bfhwtn 36 6 degree degree NN cord-304685-s0bfhwtn 36 7 of of IN cord-304685-s0bfhwtn 36 8 DNA dna NN cord-304685-s0bfhwtn 36 9 fragmentation fragmentation NN cord-304685-s0bfhwtn 36 10 was be VBD cord-304685-s0bfhwtn 36 11 related relate VBN cord-304685-s0bfhwtn 36 12 to to IN cord-304685-s0bfhwtn 36 13 the the DT cord-304685-s0bfhwtn 36 14 proliferative proliferative JJ cord-304685-s0bfhwtn 36 15 status status NN cord-304685-s0bfhwtn 36 16 of of IN cord-304685-s0bfhwtn 36 17 the the DT cord-304685-s0bfhwtn 36 18 target target NN cord-304685-s0bfhwtn 36 19 cell cell NN cord-304685-s0bfhwtn 36 20 . . . cord-304685-s0bfhwtn 37 1 More more RBR cord-304685-s0bfhwtn 37 2 importantly importantly RB cord-304685-s0bfhwtn 37 3 , , , cord-304685-s0bfhwtn 37 4 however however RB cord-304685-s0bfhwtn 37 5 , , , cord-304685-s0bfhwtn 37 6 this this DT cord-304685-s0bfhwtn 37 7 experiment experiment NN cord-304685-s0bfhwtn 37 8 established establish VBD cord-304685-s0bfhwtn 37 9 , , , cord-304685-s0bfhwtn 37 10 without without IN cord-304685-s0bfhwtn 37 11 any any DT cord-304685-s0bfhwtn 37 12 drug drug NN cord-304685-s0bfhwtn 37 13 - - HYPH cord-304685-s0bfhwtn 37 14 induced induce VBN cord-304685-s0bfhwtn 37 15 modulation modulation NN cord-304685-s0bfhwtn 37 16 of of IN cord-304685-s0bfhwtn 37 17 the the DT cord-304685-s0bfhwtn 37 18 target target NN cord-304685-s0bfhwtn 37 19 cell cell NN cord-304685-s0bfhwtn 37 20 , , , cord-304685-s0bfhwtn 37 21 that that IN cord-304685-s0bfhwtn 37 22 the the DT cord-304685-s0bfhwtn 37 23 process process NN cord-304685-s0bfhwtn 37 24 of of IN cord-304685-s0bfhwtn 37 25 target target NN cord-304685-s0bfhwtn 37 26 cell cell NN cord-304685-s0bfhwtn 37 27 membrane membrane NN cord-304685-s0bfhwtn 37 28 lysis lysis NN cord-304685-s0bfhwtn 37 29 induced induce VBN cord-304685-s0bfhwtn 37 30 by by IN cord-304685-s0bfhwtn 37 31 the the DT cord-304685-s0bfhwtn 37 32 CTL CTL NNP cord-304685-s0bfhwtn 37 33 lethal lethal NN cord-304685-s0bfhwtn 37 34 hit hit NN cord-304685-s0bfhwtn 37 35 is be VBZ cord-304685-s0bfhwtn 37 36 distinct distinct JJ cord-304685-s0bfhwtn 37 37 from from IN cord-304685-s0bfhwtn 37 38 the the DT cord-304685-s0bfhwtn 37 39 process process NN cord-304685-s0bfhwtn 37 40 of of IN cord-304685-s0bfhwtn 37 41 CTLinduced ctlinduce VBN cord-304685-s0bfhwtn 37 42 DNA dna NN cord-304685-s0bfhwtn 37 43 fragmentation fragmentation NN cord-304685-s0bfhwtn 37 44 . . . cord-304685-s0bfhwtn 38 1 We -PRON- PRP cord-304685-s0bfhwtn 38 2 next next RB cord-304685-s0bfhwtn 38 3 sought seek VBD cord-304685-s0bfhwtn 38 4 to to TO cord-304685-s0bfhwtn 38 5 determine determine VB cord-304685-s0bfhwtn 38 6 the the DT cord-304685-s0bfhwtn 38 7 susceptibility susceptibility NN cord-304685-s0bfhwtn 38 8 of of IN cord-304685-s0bfhwtn 38 9 serumstarved serumstarved JJ cord-304685-s0bfhwtn 38 10 quiescent quiescent JJ cord-304685-s0bfhwtn 38 11 calls call NNS cord-304685-s0bfhwtn 38 12 to to IN cord-304685-s0bfhwtn 38 13 CTL CTL NNP cord-304685-s0bfhwtn 38 14 - - HYPH cord-304685-s0bfhwtn 38 15 induced induce VBN cord-304685-s0bfhwtn 38 16 DNA dna NN cord-304685-s0bfhwtn 38 17 fragmentation fragmentation NN cord-304685-s0bfhwtn 38 18 . . . cord-304685-s0bfhwtn 39 1 These these DT cord-304685-s0bfhwtn 39 2 experiments experiment NNS cord-304685-s0bfhwtn 39 3 required require VBD cord-304685-s0bfhwtn 39 4 that that IN cord-304685-s0bfhwtn 39 5 the the DT cord-304685-s0bfhwtn 39 6 target target NN cord-304685-s0bfhwtn 39 7 cells cell NNS cord-304685-s0bfhwtn 39 8 be be VBP cord-304685-s0bfhwtn 39 9 able able JJ cord-304685-s0bfhwtn 39 10 to to TO cord-304685-s0bfhwtn 39 11 reach reach VB cord-304685-s0bfhwtn 39 12 a a DT cord-304685-s0bfhwtn 39 13 state state NN cord-304685-s0bfhwtn 39 14 of of IN cord-304685-s0bfhwtn 39 15 quiescence quiescence NN cord-304685-s0bfhwtn 39 16 upon upon IN cord-304685-s0bfhwtn 39 17 serum serum NN cord-304685-s0bfhwtn 39 18 deprivation deprivation NN cord-304685-s0bfhwtn 39 19 or or CC cord-304685-s0bfhwtn 39 20 growth growth NN cord-304685-s0bfhwtn 39 21 factor factor NN cord-304685-s0bfhwtn 39 22 removal removal NN cord-304685-s0bfhwtn 39 23 . . . cord-304685-s0bfhwtn 40 1 Initial initial JJ cord-304685-s0bfhwtn 40 2 experiments experiment NNS cord-304685-s0bfhwtn 40 3 with with IN cord-304685-s0bfhwtn 40 4 prototypic prototypic JJ cord-304685-s0bfhwtn 40 5 apoptotic apoptotic JJ cord-304685-s0bfhwtn 40 6 CTL CTL NNP cord-304685-s0bfhwtn 40 7 targets target NNS cord-304685-s0bfhwtn 40 8 , , , cord-304685-s0bfhwtn 40 9 i.e. i.e. FW cord-304685-s0bfhwtn 40 10 , , , cord-304685-s0bfhwtn 40 11 the the DT cord-304685-s0bfhwtn 40 12 P815 P815 NNP cord-304685-s0bfhwtn 40 13 mastocytoma mastocytoma NNP cord-304685-s0bfhwtn 40 14 , , , cord-304685-s0bfhwtn 40 15 the the DT cord-304685-s0bfhwtn 40 16 A20 A20 NNP cord-304685-s0bfhwtn 40 17 B B NNP cord-304685-s0bfhwtn 40 18 cell cell NN cord-304685-s0bfhwtn 40 19 lymphoma lymphoma NN cord-304685-s0bfhwtn 40 20 , , , cord-304685-s0bfhwtn 40 21 and and CC cord-304685-s0bfhwtn 40 22 the the DT cord-304685-s0bfhwtn 40 23 T T NNP cord-304685-s0bfhwtn 40 24 cell cell NN cord-304685-s0bfhwtn 40 25 lymphomas lymphoma NNS cord-304685-s0bfhwtn 40 26 YAC-1 YAC-1 NNP cord-304685-s0bfhwtn 40 27 and and CC cord-304685-s0bfhwtn 40 28 EL-4 EL-4 NNP cord-304685-s0bfhwtn 40 29 ( ( -LRB- cord-304685-s0bfhwtn 40 30 9 9 CD cord-304685-s0bfhwtn 40 31 ) ) -RRB- cord-304685-s0bfhwtn 40 32 proved prove VBN cord-304685-s0bfhwtn 40 33 unsuitable unsuitable JJ cord-304685-s0bfhwtn 40 34 , , , cord-304685-s0bfhwtn 40 35 as as IN cord-304685-s0bfhwtn 40 36 these these DT cord-304685-s0bfhwtn 40 37 cells cell NNS cord-304685-s0bfhwtn 40 38 underwent undergo VBD cord-304685-s0bfhwtn 40 39 serum serum NN cord-304685-s0bfhwtn 40 40 deprivation deprivation NN cord-304685-s0bfhwtn 40 41 - - HYPH cord-304685-s0bfhwtn 40 42 induced induce VBN cord-304685-s0bfhwtn 40 43 apoptosis apoptosis NN cord-304685-s0bfhwtn 40 44 during during IN cord-304685-s0bfhwtn 40 45 our -PRON- PRP$ cord-304685-s0bfhwtn 40 46 attempts attempt NNS cord-304685-s0bfhwtn 40 47 to to TO cord-304685-s0bfhwtn 40 48 render render VB cord-304685-s0bfhwtn 40 49 them -PRON- PRP cord-304685-s0bfhwtn 40 50 quiescent quiescent JJ cord-304685-s0bfhwtn 40 51 . . . cord-304685-s0bfhwtn 41 1 L929 L929 NNP cord-304685-s0bfhwtn 41 2 cells cell NNS cord-304685-s0bfhwtn 41 3 have have VBP cord-304685-s0bfhwtn 41 4 been be VBN cord-304685-s0bfhwtn 41 5 reported report VBN cord-304685-s0bfhwtn 41 6 to to TO cord-304685-s0bfhwtn 41 7 enter enter VB cord-304685-s0bfhwtn 41 8 a a DT cord-304685-s0bfhwtn 41 9 G0 g0 NN cord-304685-s0bfhwtn 41 10 - - HYPH cord-304685-s0bfhwtn 41 11 1ike 1ike CD cord-304685-s0bfhwtn 41 12 state state NN cord-304685-s0bfhwtn 41 13 after after IN cord-304685-s0bfhwtn 41 14 prolonged prolonged JJ cord-304685-s0bfhwtn 41 15 serum serum NN cord-304685-s0bfhwtn 41 16 deprivation deprivation NN cord-304685-s0bfhwtn 41 17 ( ( -LRB- cord-304685-s0bfhwtn 41 18 10 10 CD cord-304685-s0bfhwtn 41 19 ) ) -RRB- cord-304685-s0bfhwtn 41 20 , , , cord-304685-s0bfhwtn 41 21 but but CC cord-304685-s0bfhwtn 41 22 in in IN cord-304685-s0bfhwtn 41 23 our -PRON- PRP$ cord-304685-s0bfhwtn 41 24 hands hand NNS cord-304685-s0bfhwtn 41 25 the the DT cord-304685-s0bfhwtn 41 26 L929 l929 CD cord-304685-s0bfhwtn 41 27 cells cell NNS cord-304685-s0bfhwtn 41 28 displayed display VBD cord-304685-s0bfhwtn 41 29 significant significant JJ cord-304685-s0bfhwtn 41 30 levels level NNS cord-304685-s0bfhwtn 41 31 of of IN cord-304685-s0bfhwtn 41 32 DNA dna NN cord-304685-s0bfhwtn 41 33 synthesis synthesis NN cord-304685-s0bfhwtn 41 34 even even RB cord-304685-s0bfhwtn 41 35 after after IN cord-304685-s0bfhwtn 41 36 a a DT cord-304685-s0bfhwtn 41 37 3-d 3-d JJ cord-304685-s0bfhwtn 41 38 serum serum NN cord-304685-s0bfhwtn 41 39 starvation starvation NN cord-304685-s0bfhwtn 41 40 protocol protocol NN cord-304685-s0bfhwtn 41 41 . . . cord-304685-s0bfhwtn 42 1 We -PRON- PRP cord-304685-s0bfhwtn 42 2 , , , cord-304685-s0bfhwtn 42 3 therefore therefore RB cord-304685-s0bfhwtn 42 4 , , , cord-304685-s0bfhwtn 42 5 selected select VBN cord-304685-s0bfhwtn 42 6 for for IN cord-304685-s0bfhwtn 42 7 these these DT cord-304685-s0bfhwtn 42 8 studies study NNS cord-304685-s0bfhwtn 42 9 the the DT cord-304685-s0bfhwtn 42 10 NIH NIH NNP cord-304685-s0bfhwtn 42 11 BALB BALB NNP cord-304685-s0bfhwtn 42 12 / / SYM cord-304685-s0bfhwtn 42 13 c c NN cord-304685-s0bfhwtn 42 14 3T3 3t3 CD cord-304685-s0bfhwtn 42 15 cell cell NN cord-304685-s0bfhwtn 42 16 , , , cord-304685-s0bfhwtn 42 17 clone clone NN cord-304685-s0bfhwtn 42 18 A31 A31 NNP cord-304685-s0bfhwtn 42 19 ( ( -LRB- cord-304685-s0bfhwtn 42 20 3T3-A31 3T3-A31 NNP cord-304685-s0bfhwtn 42 21 ) ) -RRB- cord-304685-s0bfhwtn 42 22 , , , cord-304685-s0bfhwtn 42 23 which which WDT cord-304685-s0bfhwtn 42 24 would would MD cord-304685-s0bfhwtn 42 25 undergo undergo VB cord-304685-s0bfhwtn 42 26 quiescence quiescence NN cord-304685-s0bfhwtn 42 27 upon upon IN cord-304685-s0bfhwtn 42 28 serum serum NN cord-304685-s0bfhwtn 42 29 deprivation deprivation NN cord-304685-s0bfhwtn 42 30 ( ( -LRB- cord-304685-s0bfhwtn 42 31 11 11 CD cord-304685-s0bfhwtn 42 32 ) ) -RRB- cord-304685-s0bfhwtn 42 33 . . . cord-304685-s0bfhwtn 43 1 3T3 3t3 CD cord-304685-s0bfhwtn 43 2 cells cell NNS cord-304685-s0bfhwtn 43 3 are be VBP cord-304685-s0bfhwtn 43 4 reported report VBN cord-304685-s0bfhwtn 43 5 not not RB cord-304685-s0bfhwtn 43 6 to to TO cord-304685-s0bfhwtn 43 7 undergo undergo VB cord-304685-s0bfhwtn 43 8 high high JJ cord-304685-s0bfhwtn 43 9 levels level NNS cord-304685-s0bfhwtn 43 10 of of IN cord-304685-s0bfhwtn 43 11 DNA dna NN cord-304685-s0bfhwtn 43 12 fragmentation fragmentation NN cord-304685-s0bfhwtn 43 13 when when WRB cord-304685-s0bfhwtn 43 14 lethally lethally RB cord-304685-s0bfhwtn 43 15 hit hit VBN cord-304685-s0bfhwtn 43 16 by by IN cord-304685-s0bfhwtn 43 17 CTLs ctl NNS cord-304685-s0bfhwtn 43 18 ( ( -LRB- cord-304685-s0bfhwtn 43 19 9 9 CD cord-304685-s0bfhwtn 43 20 ) ) -RRB- cord-304685-s0bfhwtn 43 21 . . . cord-304685-s0bfhwtn 44 1 We -PRON- PRP cord-304685-s0bfhwtn 44 2 found find VBD cord-304685-s0bfhwtn 44 3 , , , cord-304685-s0bfhwtn 44 4 however however RB cord-304685-s0bfhwtn 44 5 , , , cord-304685-s0bfhwtn 44 6 that that IN cord-304685-s0bfhwtn 44 7 , , , cord-304685-s0bfhwtn 44 8 in in IN cord-304685-s0bfhwtn 44 9 9 9 CD cord-304685-s0bfhwtn 44 10 - - SYM cord-304685-s0bfhwtn 44 11 12-h 12-h CD cord-304685-s0bfhwtn 44 12 assays assay NNS cord-304685-s0bfhwtn 44 13 , , , cord-304685-s0bfhwtn 44 14 lethally lethally RB cord-304685-s0bfhwtn 44 15 hit hit VBD cord-304685-s0bfhwtn 44 16 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 44 17 cells cell NNS cord-304685-s0bfhwtn 44 18 did do VBD cord-304685-s0bfhwtn 44 19 undergo undergo VB cord-304685-s0bfhwtn 44 20 apoptosis apoptosis NN cord-304685-s0bfhwtn 44 21 , , , cord-304685-s0bfhwtn 44 22 which which WDT cord-304685-s0bfhwtn 44 23 allowed allow VBD cord-304685-s0bfhwtn 44 24 for for IN cord-304685-s0bfhwtn 44 25 tenable tenable JJ cord-304685-s0bfhwtn 44 26 DNA dna NN cord-304685-s0bfhwtn 44 27 fragmentation fragmentation NN cord-304685-s0bfhwtn 44 28 comparisons comparison NNS cord-304685-s0bfhwtn 44 29 . . . cord-304685-s0bfhwtn 45 1 When when WRB cord-304685-s0bfhwtn 45 2 quiescent quiescent JJ cord-304685-s0bfhwtn 45 3 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 45 4 cells cell NNS cord-304685-s0bfhwtn 45 5 were be VBD cord-304685-s0bfhwtn 45 6 exposed expose VBN cord-304685-s0bfhwtn 45 7 to to IN cord-304685-s0bfhwtn 45 8 LCMV LCMV NNP cord-304685-s0bfhwtn 45 9 - - HYPH cord-304685-s0bfhwtn 45 10 CTLs CTLs NNP cord-304685-s0bfhwtn 45 11 , , , cord-304685-s0bfhwtn 45 12 the the DT cord-304685-s0bfhwtn 45 13 level level NN cord-304685-s0bfhwtn 45 14 of of IN cord-304685-s0bfhwtn 45 15 membrane membrane NN cord-304685-s0bfhwtn 45 16 lysis lysis NN cord-304685-s0bfhwtn 45 17 was be VBD cord-304685-s0bfhwtn 45 18 similar similar JJ cord-304685-s0bfhwtn 45 19 to to IN cord-304685-s0bfhwtn 45 20 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 45 21 cells cell NNS cord-304685-s0bfhwtn 45 22 in in IN cord-304685-s0bfhwtn 45 23 log log NN cord-304685-s0bfhwtn 45 24 phase phase NN cord-304685-s0bfhwtn 45 25 growth growth NN cord-304685-s0bfhwtn 45 26 ( ( -LRB- cord-304685-s0bfhwtn 45 27 Fig fig NN cord-304685-s0bfhwtn 45 28 . . NNP cord-304685-s0bfhwtn 45 29 2 2 CD cord-304685-s0bfhwtn 45 30 _SP cord-304685-s0bfhwtn 45 31 A A NNP cord-304685-s0bfhwtn 45 32 ) ) -RRB- cord-304685-s0bfhwtn 45 33 , , , cord-304685-s0bfhwtn 45 34 but but CC cord-304685-s0bfhwtn 45 35 the the DT cord-304685-s0bfhwtn 45 36 level level NN cord-304685-s0bfhwtn 45 37 of of IN cord-304685-s0bfhwtn 45 38 DNA dna NN cord-304685-s0bfhwtn 45 39 fragmentation fragmentation NN cord-304685-s0bfhwtn 45 40 was be VBD cord-304685-s0bfhwtn 45 41 dramatically dramatically RB cord-304685-s0bfhwtn 45 42 lower low JJR cord-304685-s0bfhwtn 46 1 ( ( -LRB- cord-304685-s0bfhwtn 46 2 Fig Fig NNP cord-304685-s0bfhwtn 46 3 . . NNP cord-304685-s0bfhwtn 46 4 2 2 CD cord-304685-s0bfhwtn 46 5 B b NN cord-304685-s0bfhwtn 46 6 ) ) -RRB- cord-304685-s0bfhwtn 46 7 . . . cord-304685-s0bfhwtn 47 1 When when WRB cord-304685-s0bfhwtn 47 2 quiescent quiescent JJ cord-304685-s0bfhwtn 47 3 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 47 4 cells cell NNS cord-304685-s0bfhwtn 47 5 were be VBD cord-304685-s0bfhwtn 47 6 assayed assay VBN cord-304685-s0bfhwtn 47 7 in in IN cord-304685-s0bfhwtn 47 8 the the DT cord-304685-s0bfhwtn 47 9 presence presence NN cord-304685-s0bfhwtn 47 10 of of IN cord-304685-s0bfhwtn 47 11 10 10 CD cord-304685-s0bfhwtn 47 12 % % NN cord-304685-s0bfhwtn 47 13 FBS FBS NNP cord-304685-s0bfhwtn 47 14 , , , cord-304685-s0bfhwtn 47 15 added add VBD cord-304685-s0bfhwtn 47 16 at at IN cord-304685-s0bfhwtn 47 17 the the DT cord-304685-s0bfhwtn 47 18 beginning beginning NN cord-304685-s0bfhwtn 47 19 of of IN cord-304685-s0bfhwtn 47 20 the the DT cord-304685-s0bfhwtn 47 21 cytotoxicity cytotoxicity NN cord-304685-s0bfhwtn 47 22 assay assay NN cord-304685-s0bfhwtn 47 23 , , . cord-304685-s0bfhwtn 48 1 the the DT cord-304685-s0bfhwtn 48 2 level level NN cord-304685-s0bfhwtn 48 3 of of IN cord-304685-s0bfhwtn 48 4 lysis lysis NN cord-304685-s0bfhwtn 48 5 ( ( -LRB- cord-304685-s0bfhwtn 48 6 Fig fig NN cord-304685-s0bfhwtn 48 7 . . . cord-304685-s0bfhwtn 48 8 2 2 CD cord-304685-s0bfhwtn 48 9 A a CD cord-304685-s0bfhwtn 48 10 ) ) -RRB- cord-304685-s0bfhwtn 48 11 and and CC cord-304685-s0bfhwtn 48 12 the the DT cord-304685-s0bfhwtn 48 13 level level NN cord-304685-s0bfhwtn 48 14 of of IN cord-304685-s0bfhwtn 48 15 DNA dna NN cord-304685-s0bfhwtn 48 16 fragmentation fragmentation NN cord-304685-s0bfhwtn 48 17 increased increase VBD cord-304685-s0bfhwtn 48 18 only only RB cord-304685-s0bfhwtn 48 19 slightly slightly RB cord-304685-s0bfhwtn 48 20 ( ( -LRB- cord-304685-s0bfhwtn 48 21 Fig fig NN cord-304685-s0bfhwtn 48 22 . . . cord-304685-s0bfhwtn 49 1 2 2 CD cord-304685-s0bfhwtn 49 2 _SP cord-304685-s0bfhwtn 49 3 B B NNP cord-304685-s0bfhwtn 49 4 ) ) -RRB- cord-304685-s0bfhwtn 49 5 . . . cord-304685-s0bfhwtn 50 1 These these DT cord-304685-s0bfhwtn 50 2 results result NNS cord-304685-s0bfhwtn 50 3 support support VBP cord-304685-s0bfhwtn 50 4 the the DT cord-304685-s0bfhwtn 50 5 concept concept NN cord-304685-s0bfhwtn 50 6 that that IN cord-304685-s0bfhwtn 50 7 the the DT cord-304685-s0bfhwtn 50 8 DNA dna NN cord-304685-s0bfhwtn 50 9 fragmentation fragmentation NN cord-304685-s0bfhwtn 50 10 of of IN cord-304685-s0bfhwtn 50 11 the the DT cord-304685-s0bfhwtn 50 12 target target NN cord-304685-s0bfhwtn 50 13 cell cell NN cord-304685-s0bfhwtn 50 14 may may MD cord-304685-s0bfhwtn 50 15 be be VB cord-304685-s0bfhwtn 50 16 mediated mediate VBN cord-304685-s0bfhwtn 50 17 by by IN cord-304685-s0bfhwtn 50 18 a a DT cord-304685-s0bfhwtn 50 19 mechanism(s mechanism(s NNP cord-304685-s0bfhwtn 50 20 ) ) -RRB- cord-304685-s0bfhwtn 50 21 regulated regulate VBN cord-304685-s0bfhwtn 50 22 by by IN cord-304685-s0bfhwtn 50 23 cell cell NN cord-304685-s0bfhwtn 50 24 cycle cycle NN cord-304685-s0bfhwtn 50 25 controls control NNS cord-304685-s0bfhwtn 50 26 . . . cord-304685-s0bfhwtn 51 1 c c NNP cord-304685-s0bfhwtn 51 2 - - HYPH cord-304685-s0bfhwtn 51 3 myc myc NNP cord-304685-s0bfhwtn 51 4 is be VBZ cord-304685-s0bfhwtn 51 5 an an DT cord-304685-s0bfhwtn 51 6 immediate immediate JJ cord-304685-s0bfhwtn 51 7 early early JJ cord-304685-s0bfhwtn 51 8 gene gene NN cord-304685-s0bfhwtn 51 9 that that WDT cord-304685-s0bfhwtn 51 10 is be VBZ cord-304685-s0bfhwtn 51 11 expressed express VBN cord-304685-s0bfhwtn 51 12 as as IN cord-304685-s0bfhwtn 51 13 cells cell NNS cord-304685-s0bfhwtn 51 14 gain gain VBP cord-304685-s0bfhwtn 51 15 competence competence NN cord-304685-s0bfhwtn 51 16 to to TO cord-304685-s0bfhwtn 51 17 exit exit NN cord-304685-s0bfhwtn 51 18 Go go VB cord-304685-s0bfhwtn 51 19 and and CC cord-304685-s0bfhwtn 51 20 progress progress VB cord-304685-s0bfhwtn 51 21 into into IN cord-304685-s0bfhwtn 51 22 Gt Gt NNP cord-304685-s0bfhwtn 51 23 ( ( -LRB- cord-304685-s0bfhwtn 51 24 12 12 CD cord-304685-s0bfhwtn 51 25 ) ) -RRB- cord-304685-s0bfhwtn 51 26 ( ( -LRB- cord-304685-s0bfhwtn 51 27 13 13 CD cord-304685-s0bfhwtn 51 28 ) ) -RRB- cord-304685-s0bfhwtn 51 29 ( ( -LRB- cord-304685-s0bfhwtn 51 30 14 14 CD cord-304685-s0bfhwtn 51 31 ) ) -RRB- cord-304685-s0bfhwtn 51 32 . . . cord-304685-s0bfhwtn 52 1 Cells cell NNS cord-304685-s0bfhwtn 52 2 expressing express VBG cord-304685-s0bfhwtn 52 3 high high JJ cord-304685-s0bfhwtn 52 4 levels level NNS cord-304685-s0bfhwtn 52 5 of of IN cord-304685-s0bfhwtn 52 6 myc myc NNP cord-304685-s0bfhwtn 52 7 proliferate proliferate VBP cord-304685-s0bfhwtn 52 8 at at IN cord-304685-s0bfhwtn 52 9 a a DT cord-304685-s0bfhwtn 52 10 faster fast JJR cord-304685-s0bfhwtn 52 11 rate rate NN cord-304685-s0bfhwtn 52 12 than than IN cord-304685-s0bfhwtn 52 13 their -PRON- PRP$ cord-304685-s0bfhwtn 52 14 normal normal JJ cord-304685-s0bfhwtn 52 15 untransformed untransformed JJ cord-304685-s0bfhwtn 52 16 counterparts counterpart NNS cord-304685-s0bfhwtn 52 17 and and CC cord-304685-s0bfhwtn 52 18 bypass bypass NN cord-304685-s0bfhwtn 52 19 Go go VB cord-304685-s0bfhwtn 52 20 by by IN cord-304685-s0bfhwtn 52 21 entering enter VBG cord-304685-s0bfhwtn 52 22 G1 G1 NNP cord-304685-s0bfhwtn 52 23 from from IN cord-304685-s0bfhwtn 52 24 mitosis mitosis NN cord-304685-s0bfhwtn 52 25 ( ( -LRB- cord-304685-s0bfhwtn 52 26 12 12 CD cord-304685-s0bfhwtn 52 27 ) ) -RRB- cord-304685-s0bfhwtn 52 28 ( ( -LRB- cord-304685-s0bfhwtn 52 29 13 13 CD cord-304685-s0bfhwtn 52 30 ) ) -RRB- cord-304685-s0bfhwtn 52 31 ( ( -LRB- cord-304685-s0bfhwtn 52 32 14 14 CD cord-304685-s0bfhwtn 52 33 ) ) -RRB- cord-304685-s0bfhwtn 52 34 . . . cord-304685-s0bfhwtn 53 1 If if IN cord-304685-s0bfhwtn 53 2 the the DT cord-304685-s0bfhwtn 53 3 Go Go NNP cord-304685-s0bfhwtn 53 4 state state NN cord-304685-s0bfhwtn 53 5 protects protect VBZ cord-304685-s0bfhwtn 53 6 cells cell NNS cord-304685-s0bfhwtn 53 7 from from IN cord-304685-s0bfhwtn 53 8 CTL CTL NNP cord-304685-s0bfhwtn 53 9 - - HYPH cord-304685-s0bfhwtn 53 10 induced induce VBN cord-304685-s0bfhwtn 53 11 apoptosis apoptosis NN cord-304685-s0bfhwtn 53 12 , , , cord-304685-s0bfhwtn 53 13 as as IN cord-304685-s0bfhwtn 53 14 it -PRON- PRP cord-304685-s0bfhwtn 53 15 does do VBZ cord-304685-s0bfhwtn 53 16 in in IN cord-304685-s0bfhwtn 53 17 growth growth NN cord-304685-s0bfhwtn 53 18 factor factor NN cord-304685-s0bfhwtn 53 19 deprivation deprivation NN cord-304685-s0bfhwtn 53 20 - - HYPH cord-304685-s0bfhwtn 53 21 and and CC cord-304685-s0bfhwtn 53 22 p53-induced p53-induced JJ cord-304685-s0bfhwtn 53 23 apoptosis apoptosis NN cord-304685-s0bfhwtn 53 24 ( ( -LRB- cord-304685-s0bfhwtn 53 25 15 15 CD cord-304685-s0bfhwtn 53 26 , , , cord-304685-s0bfhwtn 53 27 16 16 CD cord-304685-s0bfhwtn 53 28 ) ) -RRB- cord-304685-s0bfhwtn 53 29 , , , cord-304685-s0bfhwtn 53 30 one one PRP cord-304685-s0bfhwtn 53 31 would would MD cord-304685-s0bfhwtn 53 32 predict predict VB cord-304685-s0bfhwtn 53 33 that that IN cord-304685-s0bfhwtn 53 34 c c NN cord-304685-s0bfhwtn 53 35 - - HYPH cord-304685-s0bfhwtn 53 36 myc myc NNP cord-304685-s0bfhwtn 53 37 - - HYPH cord-304685-s0bfhwtn 53 38 transformed transform VBN cord-304685-s0bfhwtn 53 39 cells cell NNS cord-304685-s0bfhwtn 53 40 would would MD cord-304685-s0bfhwtn 53 41 be be VB cord-304685-s0bfhwtn 53 42 more more RBR cord-304685-s0bfhwtn 53 43 susceptible susceptible JJ cord-304685-s0bfhwtn 53 44 to to IN cord-304685-s0bfhwtn 53 45 apoptosis apoptosis NN cord-304685-s0bfhwtn 53 46 because because IN cord-304685-s0bfhwtn 53 47 they -PRON- PRP cord-304685-s0bfhwtn 53 48 do do VBP cord-304685-s0bfhwtn 53 49 not not RB cord-304685-s0bfhwtn 53 50 enter enter VB cord-304685-s0bfhwtn 53 51 Go go VB cord-304685-s0bfhwtn 53 52 . . . cord-304685-s0bfhwtn 54 1 We -PRON- PRP cord-304685-s0bfhwtn 54 2 transinfected transinfecte VBD cord-304685-s0bfhwtn 54 3 the the DT cord-304685-s0bfhwtn 54 4 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 54 5 cells cell NNS cord-304685-s0bfhwtn 54 6 with with IN cord-304685-s0bfhwtn 54 7 a a DT cord-304685-s0bfhwtn 54 8 c c NNP cord-304685-s0bfhwtn 54 9 - - HYPH cord-304685-s0bfhwtn 54 10 myc myc NNP cord-304685-s0bfhwtn 54 11 containing contain VBG cord-304685-s0bfhwtn 54 12 retrovirus retrovirus NN cord-304685-s0bfhwtn 54 13 ( ( -LRB- cord-304685-s0bfhwtn 54 14 17 17 CD cord-304685-s0bfhwtn 54 15 ) ) -RRB- cord-304685-s0bfhwtn 54 16 and and CC cord-304685-s0bfhwtn 54 17 selected select VBD cord-304685-s0bfhwtn 54 18 a a DT cord-304685-s0bfhwtn 54 19 transformant transformant NN cord-304685-s0bfhwtn 54 20 in in IN cord-304685-s0bfhwtn 54 21 soft soft JJ cord-304685-s0bfhwtn 54 22 agar agar NN cord-304685-s0bfhwtn 54 23 . . . cord-304685-s0bfhwtn 55 1 In in IN cord-304685-s0bfhwtn 55 2 the the DT cord-304685-s0bfhwtn 55 3 presence presence NN cord-304685-s0bfhwtn 55 4 of of IN cord-304685-s0bfhwtn 55 5 10 10 CD cord-304685-s0bfhwtn 55 6 % % NN cord-304685-s0bfhwtn 55 7 FBS FBS NNP cord-304685-s0bfhwtn 55 8 , , , cord-304685-s0bfhwtn 55 9 the the DT cord-304685-s0bfhwtn 55 10 population population NN cord-304685-s0bfhwtn 55 11 doubling double VBG cord-304685-s0bfhwtn 55 12 time time NN cord-304685-s0bfhwtn 55 13 of of IN cord-304685-s0bfhwtn 55 14 these these DT cord-304685-s0bfhwtn 55 15 cells cell NNS cord-304685-s0bfhwtn 55 16 ( ( -LRB- cord-304685-s0bfhwtn 55 17 ta ta NNP cord-304685-s0bfhwtn 55 18 ) ) -RRB- cord-304685-s0bfhwtn 55 19 was be VBD cord-304685-s0bfhwtn 56 1 12 12 CD cord-304685-s0bfhwtn 56 2 _ _ NN cord-304685-s0bfhwtn 57 1 + + CC cord-304685-s0bfhwtn 58 1 1 1 LS cord-304685-s0bfhwtn 58 2 h h NN cord-304685-s0bfhwtn 58 3 vs. vs. NN cord-304685-s0bfhwtn 58 4 20 20 CD cord-304685-s0bfhwtn 58 5 _ _ NN cord-304685-s0bfhwtn 59 1 + + CC cord-304685-s0bfhwtn 60 1 2 2 LS cord-304685-s0bfhwtn 60 2 h h NN cord-304685-s0bfhwtn 60 3 for for IN cord-304685-s0bfhwtn 60 4 the the DT cord-304685-s0bfhwtn 60 5 parental parental JJ cord-304685-s0bfhwtn 60 6 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 60 7 cells cell NNS cord-304685-s0bfhwtn 60 8 . . . cord-304685-s0bfhwtn 61 1 The the DT cord-304685-s0bfhwtn 61 2 enforced enforce VBN cord-304685-s0bfhwtn 61 3 expression expression NN cord-304685-s0bfhwtn 61 4 ofc ofc NNP cord-304685-s0bfhwtn 61 5 - - HYPH cord-304685-s0bfhwtn 61 6 myc myc NNP cord-304685-s0bfhwtn 61 7 did do VBD cord-304685-s0bfhwtn 61 8 not not RB cord-304685-s0bfhwtn 61 9 alter alter VB cord-304685-s0bfhwtn 61 10 the the DT cord-304685-s0bfhwtn 61 11 membrane membrane NN cord-304685-s0bfhwtn 61 12 lysis lysis NN cord-304685-s0bfhwtn 61 13 between between IN cord-304685-s0bfhwtn 61 14 these these DT cord-304685-s0bfhwtn 61 15 targets target NNS cord-304685-s0bfhwtn 61 16 ( ( -LRB- cord-304685-s0bfhwtn 61 17 Fig Fig NNP cord-304685-s0bfhwtn 61 18 . . . cord-304685-s0bfhwtn 61 19 3 3 CD cord-304685-s0bfhwtn 61 20 A a NN cord-304685-s0bfhwtn 61 21 ) ) -RRB- cord-304685-s0bfhwtn 61 22 but but CC cord-304685-s0bfhwtn 61 23 markedly markedly RB cord-304685-s0bfhwtn 61 24 enhanced enhance VBD cord-304685-s0bfhwtn 61 25 their -PRON- PRP$ cord-304685-s0bfhwtn 61 26 susceptibility susceptibility NN cord-304685-s0bfhwtn 61 27 to to IN cord-304685-s0bfhwtn 61 28 CTL CTL NNP cord-304685-s0bfhwtn 61 29 - - HYPH cord-304685-s0bfhwtn 61 30 induced induce VBN cord-304685-s0bfhwtn 61 31 DNA dna NN cord-304685-s0bfhwtn 61 32 fragmentation fragmentation NN cord-304685-s0bfhwtn 61 33 ( ( -LRB- cord-304685-s0bfhwtn 61 34 Fig fig NN cord-304685-s0bfhwtn 61 35 . . . cord-304685-s0bfhwtn 61 36 _SP cord-304685-s0bfhwtn 62 1 3 3 LS cord-304685-s0bfhwtn 62 2 ( ( -LRB- cord-304685-s0bfhwtn 62 3 3 3 CD cord-304685-s0bfhwtn 62 4 ) ) -RRB- cord-304685-s0bfhwtn 62 5 and and CC cord-304685-s0bfhwtn 62 6 used use VBD cord-304685-s0bfhwtn 62 7 as as IN cord-304685-s0bfhwtn 62 8 targets target NNS cord-304685-s0bfhwtn 62 9 ~ ~ NFP cord-304685-s0bfhwtn 62 10 on on IN cord-304685-s0bfhwtn 62 11 day day NN cord-304685-s0bfhwtn 62 12 0 0 CD cord-304685-s0bfhwtn 62 13 . . . cord-304685-s0bfhwtn 63 1 Just just RB cord-304685-s0bfhwtn 63 2 before before IN cord-304685-s0bfhwtn 63 3 use use NN cord-304685-s0bfhwtn 63 4 , , , cord-304685-s0bfhwtn 63 5 purified purify VBN cord-304685-s0bfhwtn 63 6 I.CMV i.cmv NN cord-304685-s0bfhwtn 63 7 - - HYPH cord-304685-s0bfhwtn 63 8 CTL CTL NNP cord-304685-s0bfhwtn 63 9 were be VBD cord-304685-s0bfhwtn 63 10 resuspended resuspend VBN cord-304685-s0bfhwtn 63 11 in in IN cord-304685-s0bfhwtn 63 12 medium medium NN cord-304685-s0bfhwtn 63 13 containing contain VBG cord-304685-s0bfhwtn 63 14 the the DT cord-304685-s0bfhwtn 63 15 desired desire VBN cord-304685-s0bfhwtn 63 16 concentration concentration NN cord-304685-s0bfhwtn 63 17 of of IN cord-304685-s0bfhwtn 63 18 FBS FBS NNP cord-304685-s0bfhwtn 63 19 . . . cord-304685-s0bfhwtn 64 1 Cytotoxicity cytotoxicity NN cord-304685-s0bfhwtn 64 2 assays assay NNS cord-304685-s0bfhwtn 64 3 were be VBD cord-304685-s0bfhwtn 64 4 performed perform VBN cord-304685-s0bfhwtn 64 5 as as IN cord-304685-s0bfhwtn 64 6 described describe VBN cord-304685-s0bfhwtn 64 7 ( ( -LRB- cord-304685-s0bfhwtn 64 8 5 5 LS cord-304685-s0bfhwtn 64 9 ) ) -RRB- cord-304685-s0bfhwtn 64 10 with with IN cord-304685-s0bfhwtn 64 11 Con Con NNP cord-304685-s0bfhwtn 64 12 A A NNP cord-304685-s0bfhwtn 64 13 ( ( -LRB- cord-304685-s0bfhwtn 64 14 Sigma Sigma NNP cord-304685-s0bfhwtn 64 15 Chemical Chemical NNP cord-304685-s0bfhwtn 64 16 Co. Co. NNP cord-304685-s0bfhwtn 64 17 ) ) -RRB- cord-304685-s0bfhwtn 64 18 at at IN cord-304685-s0bfhwtn 64 19 5/~g 5/~g CD cord-304685-s0bfhwtn 64 20 / / SYM cord-304685-s0bfhwtn 64 21 ml ml NN cord-304685-s0bfhwtn 64 22 . . . cord-304685-s0bfhwtn 65 1 L929 L929 NNP cord-304685-s0bfhwtn 65 2 cells cell NNS cord-304685-s0bfhwtn 65 3 were be VBD cord-304685-s0bfhwtn 65 4 cultured culture VBN cord-304685-s0bfhwtn 65 5 identically identically RB cord-304685-s0bfhwtn 65 6 for for IN cord-304685-s0bfhwtn 65 7 both both CC cord-304685-s0bfhwtn 65 8 the the DT cord-304685-s0bfhwtn 65 9 slCr slCr NNP cord-304685-s0bfhwtn 65 10 - - : cord-304685-s0bfhwtn 65 11 and and CC cord-304685-s0bfhwtn 65 12 125I 125I NNP cord-304685-s0bfhwtn 65 13 - - HYPH cord-304685-s0bfhwtn 65 14 IUDRrelease iudrrelease NN cord-304685-s0bfhwtn 65 15 portion portion NN cord-304685-s0bfhwtn 65 16 of of IN cord-304685-s0bfhwtn 65 17 the the DT cord-304685-s0bfhwtn 65 18 assay assay NN cord-304685-s0bfhwtn 65 19 . . . cord-304685-s0bfhwtn 66 1 Assay assay NN cord-304685-s0bfhwtn 66 2 time time NN cord-304685-s0bfhwtn 66 3 for for IN cord-304685-s0bfhwtn 66 4 A a NN cord-304685-s0bfhwtn 66 5 and and CC cord-304685-s0bfhwtn 66 6 B b NN cord-304685-s0bfhwtn 66 7 , , , cord-304685-s0bfhwtn 66 8 performed perform VBN cord-304685-s0bfhwtn 66 9 in in IN cord-304685-s0bfhwtn 66 10 parallel parallel NN cord-304685-s0bfhwtn 66 11 , , , cord-304685-s0bfhwtn 66 12 was be VBD cord-304685-s0bfhwtn 66 13 6 6 CD cord-304685-s0bfhwtn 66 14 h. h. NN cord-304685-s0bfhwtn 66 15 Standard Standard NNP cord-304685-s0bfhwtn 66 16 error error NN cord-304685-s0bfhwtn 66 17 of of IN cord-304685-s0bfhwtn 66 18 mean mean JJ cord-304685-s0bfhwtn 66 19 ( ( -LRB- cord-304685-s0bfhwtn 66 20 SEM SEM NNP cord-304685-s0bfhwtn 66 21 ) ) -RRB- cord-304685-s0bfhwtn 67 1 for for IN cord-304685-s0bfhwtn 67 2 slCr slcr CD cord-304685-s0bfhwtn 67 3 release release NN cord-304685-s0bfhwtn 67 4 was be VBD cord-304685-s0bfhwtn 67 5 < < XX cord-304685-s0bfhwtn 67 6 5 5 CD cord-304685-s0bfhwtn 67 7 % % NN cord-304685-s0bfhwtn 67 8 . . . cord-304685-s0bfhwtn 68 1 ( ( -LRB- cord-304685-s0bfhwtn 68 2 B b NN cord-304685-s0bfhwtn 68 3 ) ) -RRB- cord-304685-s0bfhwtn 68 4 Infuence infuence NN cord-304685-s0bfhwtn 68 5 of of IN cord-304685-s0bfhwtn 68 6 serum serum NN cord-304685-s0bfhwtn 68 7 on on IN cord-304685-s0bfhwtn 68 8 target target NN cord-304685-s0bfhwtn 68 9 cell cell NN cord-304685-s0bfhwtn 68 10 's 's POS cord-304685-s0bfhwtn 68 11 susceptibility susceptibility NN cord-304685-s0bfhwtn 68 12 to to IN cord-304685-s0bfhwtn 68 13 CTMnduced CTMnduced NNP cord-304685-s0bfhwtn 68 14 DNA dna NN cord-304685-s0bfhwtn 68 15 fragmentation fragmentation NN cord-304685-s0bfhwtn 68 16 . . . cord-304685-s0bfhwtn 69 1 L929 l929 JJ cord-304685-s0bfhwtn 69 2 target target NN cord-304685-s0bfhwtn 69 3 cells cell NNS cord-304685-s0bfhwtn 69 4 were be VBD cord-304685-s0bfhwtn 69 5 cultured culture VBN cord-304685-s0bfhwtn 69 6 as as IN cord-304685-s0bfhwtn 69 7 above above RB cord-304685-s0bfhwtn 69 8 and and CC cord-304685-s0bfhwtn 69 9 labeled label VBN cord-304685-s0bfhwtn 69 10 with with IN cord-304685-s0bfhwtn 69 11 [ [ -LRB- cord-304685-s0bfhwtn 69 12 125I]iododeoxyuridine 125I]iododeoxyuridine NNP cord-304685-s0bfhwtn 69 13 ( ( -LRB- cord-304685-s0bfhwtn 69 14 12sI 12si CD cord-304685-s0bfhwtn 69 15 - - HYPH cord-304685-s0bfhwtn 69 16 IUDR IUDR NNP cord-304685-s0bfhwtn 69 17 . . . cord-304685-s0bfhwtn 69 18 ) ) -RRB- cord-304685-s0bfhwtn 70 1 at at IN cord-304685-s0bfhwtn 70 2 1 1 CD cord-304685-s0bfhwtn 70 3 # # NN cord-304685-s0bfhwtn 70 4 Ci ci NN cord-304685-s0bfhwtn 70 5 / / SYM cord-304685-s0bfhwtn 70 6 ml ml NN cord-304685-s0bfhwtn 70 7 on on IN cord-304685-s0bfhwtn 70 8 day day NN cord-304685-s0bfhwtn 70 9 -3 -3 NNP cord-304685-s0bfhwtn 70 10 for for IN cord-304685-s0bfhwtn 70 11 18 18 CD cord-304685-s0bfhwtn 70 12 h h NN cord-304685-s0bfhwtn 70 13 as as IN cord-304685-s0bfhwtn 70 14 described describe VBN cord-304685-s0bfhwtn 70 15 ( ( -LRB- cord-304685-s0bfhwtn 70 16 3 3 CD cord-304685-s0bfhwtn 70 17 ) ) -RRB- cord-304685-s0bfhwtn 70 18 . . . cord-304685-s0bfhwtn 71 1 SEM SEM NNP cord-304685-s0bfhwtn 71 2 : : : cord-304685-s0bfhwtn 72 1 12SI 12si CD cord-304685-s0bfhwtn 72 2 - - HYPH cord-304685-s0bfhwtn 72 3 DNA dna NN cord-304685-s0bfhwtn 72 4 release release NN cord-304685-s0bfhwtn 72 5 < < XX cord-304685-s0bfhwtn 72 6 10 10 CD cord-304685-s0bfhwtn 72 7 % % NN cord-304685-s0bfhwtn 72 8 . . . cord-304685-s0bfhwtn 73 1 quiescent quiescent JJ cord-304685-s0bfhwtn 73 2 targets target NNS cord-304685-s0bfhwtn 73 3 with with IN cord-304685-s0bfhwtn 73 4 10 10 CD cord-304685-s0bfhwtn 73 5 % % NN cord-304685-s0bfhwtn 73 6 FBS FBS NNP cord-304685-s0bfhwtn 73 7 added add VBD cord-304685-s0bfhwtn 73 8 during during IN cord-304685-s0bfhwtn 73 9 the the DT cord-304685-s0bfhwtn 73 10 assay assay NN cord-304685-s0bfhwtn 73 11 . . . cord-304685-s0bfhwtn 74 1 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 74 2 target target NN cord-304685-s0bfhwtn 74 3 cells cell NNS cord-304685-s0bfhwtn 74 4 were be VBD cord-304685-s0bfhwtn 74 5 cultured culture VBN cord-304685-s0bfhwtn 74 6 in in IN cord-304685-s0bfhwtn 74 7 DMEM+10 DMEM+10 NNP cord-304685-s0bfhwtn 74 8 % % NN cord-304685-s0bfhwtn 74 9 calf calf NN cord-304685-s0bfhwtn 74 10 serum serum NN cord-304685-s0bfhwtn 74 11 ( ( -LRB- cord-304685-s0bfhwtn 74 12 Hyclone Hyclone NNP cord-304685-s0bfhwtn 74 13 Labs Labs NNP cord-304685-s0bfhwtn 74 14 . . NNP cord-304685-s0bfhwtn 74 15 , , , cord-304685-s0bfhwtn 74 16 Logan Logan NNP cord-304685-s0bfhwtn 74 17 , , , cord-304685-s0bfhwtn 74 18 UT UT NNP cord-304685-s0bfhwtn 74 19 ) ) -RRB- cord-304685-s0bfhwtn 74 20 . . . cord-304685-s0bfhwtn 75 1 5 5 LS cord-304685-s0bfhwtn 75 2 d d NN cord-304685-s0bfhwtn 75 3 before before IN cord-304685-s0bfhwtn 75 4 use use NN cord-304685-s0bfhwtn 75 5 ( ( -LRB- cord-304685-s0bfhwtn 75 6 day day NN cord-304685-s0bfhwtn 75 7 -5 -5 CD cord-304685-s0bfhwtn 75 8 ) ) -RRB- cord-304685-s0bfhwtn 76 1 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 76 2 cells cell NNS cord-304685-s0bfhwtn 76 3 6:1 6:1 CD cord-304685-s0bfhwtn 76 4 12:1 12:1 CD cord-304685-s0bfhwtn 76 5 25:1 25:1 CD cord-304685-s0bfhwtn 77 1 50:1 50:1 CD cord-304685-s0bfhwtn 77 2 were be VBD cord-304685-s0bfhwtn 77 3 subcultured subculture VBN cord-304685-s0bfhwtn 77 4 into into IN cord-304685-s0bfhwtn 77 5 T150 t150 NN cord-304685-s0bfhwtn 77 6 cm cm NNP cord-304685-s0bfhwtn 77 7 2 2 CD cord-304685-s0bfhwtn 77 8 Effector Effector NNP cord-304685-s0bfhwtn 77 9 to to TO cord-304685-s0bfhwtn 77 10 Target target VB cord-304685-s0bfhwtn 77 11 Ratio ratio NN cord-304685-s0bfhwtn 77 12 flasks flask NNS cord-304685-s0bfhwtn 77 13 to to TO cord-304685-s0bfhwtn 77 14 obtain obtain VB cord-304685-s0bfhwtn 77 15 , , , cord-304685-s0bfhwtn 77 16 ' ' '' cord-304685-s0bfhwtn 77 17 o75 o75 CD cord-304685-s0bfhwtn 77 18 % % NN cord-304685-s0bfhwtn 77 19 confluency confluency NN cord-304685-s0bfhwtn 77 20 , , . cord-304685-s0bfhwtn 78 1 On on IN cord-304685-s0bfhwtn 78 2 day day NN cord-304685-s0bfhwtn 78 3 -4 -4 NNP cord-304685-s0bfhwtn 78 4 , , , cord-304685-s0bfhwtn 78 5 they -PRON- PRP cord-304685-s0bfhwtn 78 6 were be VBD cord-304685-s0bfhwtn 78 7 labeled label VBN cord-304685-s0bfhwtn 78 8 with with IN cord-304685-s0bfhwtn 78 9 125I 125I NNP cord-304685-s0bfhwtn 78 10 - - HYPH cord-304685-s0bfhwtn 78 11 IUDR IUDR NNP cord-304685-s0bfhwtn 78 12 at at IN cord-304685-s0bfhwtn 78 13 1/~Ci 1/~ci CD cord-304685-s0bfhwtn 78 14 / / SYM cord-304685-s0bfhwtn 78 15 ml ml NN cord-304685-s0bfhwtn 78 16 in in IN cord-304685-s0bfhwtn 78 17 5 5 CD cord-304685-s0bfhwtn 78 18 ml ml NNS cord-304685-s0bfhwtn 78 19 for for IN cord-304685-s0bfhwtn 78 20 18 18 CD cord-304685-s0bfhwtn 78 21 h. h. NN cord-304685-s0bfhwtn 79 1 After after IN cord-304685-s0bfhwtn 79 2 labeling labeling NN cord-304685-s0bfhwtn 79 3 , , , cord-304685-s0bfhwtn 79 4 the the DT cord-304685-s0bfhwtn 79 5 medium medium NN cord-304685-s0bfhwtn 79 6 was be VBD cord-304685-s0bfhwtn 79 7 removed remove VBN cord-304685-s0bfhwtn 79 8 , , , cord-304685-s0bfhwtn 79 9 and and CC cord-304685-s0bfhwtn 79 10 fresh fresh JJ cord-304685-s0bfhwtn 79 11 RPMI RPMI NNP cord-304685-s0bfhwtn 80 1 1640 1640 CD cord-304685-s0bfhwtn 80 2 + + SYM cord-304685-s0bfhwtn 80 3 5 5 CD cord-304685-s0bfhwtn 80 4 % % NN cord-304685-s0bfhwtn 80 5 CPSR-2 cpsr-2 NN cord-304685-s0bfhwtn 80 6 ( ( -LRB- cord-304685-s0bfhwtn 80 7 Sigma Sigma NNP cord-304685-s0bfhwtn 80 8 Chemical Chemical NNP cord-304685-s0bfhwtn 80 9 Co. Co. NNP cord-304685-s0bfhwtn 80 10 ) ) -RRB- cord-304685-s0bfhwtn 80 11 was be VBD cord-304685-s0bfhwtn 80 12 added add VBN cord-304685-s0bfhwtn 80 13 , , , cord-304685-s0bfhwtn 80 14 and and CC cord-304685-s0bfhwtn 80 15 the the DT cord-304685-s0bfhwtn 80 16 cells cell NNS cord-304685-s0bfhwtn 80 17 were be VBD cord-304685-s0bfhwtn 80 18 allowed allow VBN cord-304685-s0bfhwtn 80 19 to to TO cord-304685-s0bfhwtn 80 20 quiesce quiesce VB cord-304685-s0bfhwtn 80 21 for for IN cord-304685-s0bfhwtn 80 22 a a DT cord-304685-s0bfhwtn 80 23 minimum minimum NN cord-304685-s0bfhwtn 80 24 of of IN cord-304685-s0bfhwtn 80 25 72 72 CD cord-304685-s0bfhwtn 80 26 h. h. NN cord-304685-s0bfhwtn 80 27 CPSR.-2 CPSR.-2 NNP cord-304685-s0bfhwtn 80 28 is be VBZ cord-304685-s0bfhwtn 80 29 a a DT cord-304685-s0bfhwtn 80 30 nonmitogenic nonmitogenic JJ cord-304685-s0bfhwtn 80 31 serum serum NN cord-304685-s0bfhwtn 80 32 substitute substitute NN cord-304685-s0bfhwtn 80 33 . . . cord-304685-s0bfhwtn 81 1 For for IN cord-304685-s0bfhwtn 81 2 cells cell NNS cord-304685-s0bfhwtn 81 3 in in IN cord-304685-s0bfhwtn 81 4 log log NN cord-304685-s0bfhwtn 81 5 phase phase NN cord-304685-s0bfhwtn 81 6 growth growth NN cord-304685-s0bfhwtn 81 7 , , , cord-304685-s0bfhwtn 81 8 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 81 9 cells cell NNS cord-304685-s0bfhwtn 81 10 were be VBD cord-304685-s0bfhwtn 81 11 subcultured subculture VBN cord-304685-s0bfhwtn 81 12 to to IN cord-304685-s0bfhwtn 81 13 , , , cord-304685-s0bfhwtn 81 14 ' ' '' cord-304685-s0bfhwtn 81 15 050 050 CD cord-304685-s0bfhwtn 81 16 % % NN cord-304685-s0bfhwtn 81 17 confluency confluency NNP cord-304685-s0bfhwtn 82 1 2 2 LS cord-304685-s0bfhwtn 82 2 d d NN cord-304685-s0bfhwtn 82 3 before before IN cord-304685-s0bfhwtn 82 4 use use NN cord-304685-s0bfhwtn 82 5 . . . cord-304685-s0bfhwtn 83 1 The the DT cord-304685-s0bfhwtn 83 2 cells cell NNS cord-304685-s0bfhwtn 83 3 were be VBD cord-304685-s0bfhwtn 83 4 labeled label VBN cord-304685-s0bfhwtn 83 5 as as IN cord-304685-s0bfhwtn 83 6 above above RB cord-304685-s0bfhwtn 83 7 on on IN cord-304685-s0bfhwtn 83 8 day day NN cord-304685-s0bfhwtn 83 9 -1 -1 CD cord-304685-s0bfhwtn 83 10 . . . cord-304685-s0bfhwtn 84 1 Cytotoxicity cytotoxicity NN cord-304685-s0bfhwtn 84 2 assays assay NNS cord-304685-s0bfhwtn 84 3 were be VBD cord-304685-s0bfhwtn 84 4 performed perform VBN cord-304685-s0bfhwtn 84 5 with with IN cord-304685-s0bfhwtn 84 6 I.CMV i.cmv NN cord-304685-s0bfhwtn 84 7 - - HYPH cord-304685-s0bfhwtn 84 8 CTL ctl NN cord-304685-s0bfhwtn 84 9 in in IN cord-304685-s0bfhwtn 84 10 lectin lectin NN cord-304685-s0bfhwtn 84 11 - - HYPH cord-304685-s0bfhwtn 84 12 dependent dependent JJ cord-304685-s0bfhwtn 84 13 assays assay NNS cord-304685-s0bfhwtn 84 14 in in IN cord-304685-s0bfhwtn 84 15 AIM aim NN cord-304685-s0bfhwtn 84 16 - - HYPH cord-304685-s0bfhwtn 84 17 V v NN cord-304685-s0bfhwtn 84 18 media medium NNS cord-304685-s0bfhwtn 84 19 without without IN cord-304685-s0bfhwtn 84 20 or or CC cord-304685-s0bfhwtn 84 21 with with IN cord-304685-s0bfhwtn 84 22 10 10 CD cord-304685-s0bfhwtn 84 23 % % NN cord-304685-s0bfhwtn 84 24 FBS FBS NNP cord-304685-s0bfhwtn 84 25 . . . cord-304685-s0bfhwtn 85 1 Assay assay NN cord-304685-s0bfhwtn 85 2 time time NN cord-304685-s0bfhwtn 85 3 for for IN cord-304685-s0bfhwtn 85 4 parts part NNS cord-304685-s0bfhwtn 85 5 A A NNP cord-304685-s0bfhwtn 85 6 and and CC cord-304685-s0bfhwtn 85 7 B B NNP cord-304685-s0bfhwtn 85 8 performed perform VBN cord-304685-s0bfhwtn 85 9 in in IN cord-304685-s0bfhwtn 85 10 parallel parallel NN cord-304685-s0bfhwtn 85 11 , , , cord-304685-s0bfhwtn 85 12 was be VBD cord-304685-s0bfhwtn 85 13 10 10 CD cord-304685-s0bfhwtn 85 14 h. h. NN cord-304685-s0bfhwtn 85 15 SEM SEM NNP cord-304685-s0bfhwtn 85 16 , , , cord-304685-s0bfhwtn 85 17 12SI 12si CD cord-304685-s0bfhwtn 85 18 - - HYPH cord-304685-s0bfhwtn 85 19 DNA dna NN cord-304685-s0bfhwtn 85 20 release release NN cord-304685-s0bfhwtn 85 21 < < XX cord-304685-s0bfhwtn 85 22 15 15 CD cord-304685-s0bfhwtn 85 23 % % NN cord-304685-s0bfhwtn 85 24 . . . cord-304685-s0bfhwtn 86 1 growth growth NN cord-304685-s0bfhwtn 86 2 rate rate NN cord-304685-s0bfhwtn 86 3 under under IN cord-304685-s0bfhwtn 86 4 these these DT cord-304685-s0bfhwtn 86 5 conditions condition NNS cord-304685-s0bfhwtn 86 6 , , , cord-304685-s0bfhwtn 86 7 had have VBD cord-304685-s0bfhwtn 86 8 an an DT cord-304685-s0bfhwtn 86 9 intermediate intermediate JJ cord-304685-s0bfhwtn 86 10 level level NN cord-304685-s0bfhwtn 86 11 of of IN cord-304685-s0bfhwtn 86 12 DNA dna NN cord-304685-s0bfhwtn 86 13 fragmentation fragmentation NN cord-304685-s0bfhwtn 86 14 even even RB cord-304685-s0bfhwtn 86 15 though though IN cord-304685-s0bfhwtn 86 16 they -PRON- PRP cord-304685-s0bfhwtn 86 17 showed show VBD cord-304685-s0bfhwtn 86 18 a a DT cord-304685-s0bfhwtn 86 19 lower low JJR cord-304685-s0bfhwtn 86 20 levd levd NN cord-304685-s0bfhwtn 86 21 of of IN cord-304685-s0bfhwtn 86 22 membrane membrane NN cord-304685-s0bfhwtn 86 23 lysis lysis NN cord-304685-s0bfhwtn 86 24 than than IN cord-304685-s0bfhwtn 86 25 the the DT cord-304685-s0bfhwtn 86 26 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 86 27 cells cell NNS cord-304685-s0bfhwtn 86 28 ( ( -LRB- cord-304685-s0bfhwtn 86 29 Fig Fig NNP cord-304685-s0bfhwtn 86 30 . . . cord-304685-s0bfhwtn 86 31 3 3 CD cord-304685-s0bfhwtn 86 32 , , , cord-304685-s0bfhwtn 86 33 A a DT cord-304685-s0bfhwtn 86 34 _SP cord-304685-s0bfhwtn 86 35 and and CC cord-304685-s0bfhwtn 86 36 B B NNP cord-304685-s0bfhwtn 86 37 ) ) -RRB- cord-304685-s0bfhwtn 86 38 . . . cord-304685-s0bfhwtn 87 1 To to TO cord-304685-s0bfhwtn 87 2 further further RB cord-304685-s0bfhwtn 87 3 dissect dissect VB cord-304685-s0bfhwtn 87 4 the the DT cord-304685-s0bfhwtn 87 5 relationship relationship NN cord-304685-s0bfhwtn 87 6 between between IN cord-304685-s0bfhwtn 87 7 the the DT cord-304685-s0bfhwtn 87 8 proliferative proliferative JJ cord-304685-s0bfhwtn 87 9 status status NN cord-304685-s0bfhwtn 87 10 of of IN cord-304685-s0bfhwtn 87 11 the the DT cord-304685-s0bfhwtn 87 12 target target NN cord-304685-s0bfhwtn 87 13 cell cell NN cord-304685-s0bfhwtn 87 14 and and CC cord-304685-s0bfhwtn 87 15 CTL CTL NNP cord-304685-s0bfhwtn 87 16 - - HYPH cord-304685-s0bfhwtn 87 17 induced induce VBN cord-304685-s0bfhwtn 87 18 DNA dna NN cord-304685-s0bfhwtn 87 19 fragmentation fragmentation NN cord-304685-s0bfhwtn 87 20 , , , cord-304685-s0bfhwtn 87 21 we -PRON- PRP cord-304685-s0bfhwtn 87 22 analyzed analyze VBD cord-304685-s0bfhwtn 87 23 CTL CTL NNP cord-304685-s0bfhwtn 87 24 - - HYPH cord-304685-s0bfhwtn 87 25 induced induce VBN cord-304685-s0bfhwtn 87 26 apoptosis apoptosis NN cord-304685-s0bfhwtn 87 27 in in IN cord-304685-s0bfhwtn 87 28 the the DT cord-304685-s0bfhwtn 87 29 prototypic prototypic JJ cord-304685-s0bfhwtn 87 30 targets target NNS cord-304685-s0bfhwtn 87 31 for for IN cord-304685-s0bfhwtn 87 32 apoptosis apoptosis NN cord-304685-s0bfhwtn 87 33 , , , cord-304685-s0bfhwtn 87 34 P815 P815 '' cord-304685-s0bfhwtn 87 35 ( ( -LRB- cord-304685-s0bfhwtn 87 36 ta ta XX cord-304685-s0bfhwtn 88 1 = = SYM cord-304685-s0bfhwtn 88 2 11 11 CD cord-304685-s0bfhwtn 88 3 _ _ NN cord-304685-s0bfhwtn 88 4 + + SYM cord-304685-s0bfhwtn 88 5 2 2 CD cord-304685-s0bfhwtn 88 6 h h NN cord-304685-s0bfhwtn 88 7 ) ) -RRB- cord-304685-s0bfhwtn 88 8 and and CC cord-304685-s0bfhwtn 89 1 A20 A20 NNP cord-304685-s0bfhwtn 90 1 ( ( -LRB- cord-304685-s0bfhwtn 90 2 td td NNP cord-304685-s0bfhwtn 90 3 = = SYM cord-304685-s0bfhwtn 90 4 14 14 CD cord-304685-s0bfhwtn 90 5 _ _ NN cord-304685-s0bfhwtn 90 6 + + SYM cord-304685-s0bfhwtn 90 7 1 1 CD cord-304685-s0bfhwtn 90 8 h h NN cord-304685-s0bfhwtn 90 9 ) ) -RRB- cord-304685-s0bfhwtn 90 10 cells cell NNS cord-304685-s0bfhwtn 90 11 . . . cord-304685-s0bfhwtn 91 1 These these DT cord-304685-s0bfhwtn 91 2 suspension suspension NN cord-304685-s0bfhwtn 91 3 - - HYPH cord-304685-s0bfhwtn 91 4 grown grow VBN cord-304685-s0bfhwtn 91 5 cells cell NNS cord-304685-s0bfhwtn 91 6 were be VBD cord-304685-s0bfhwtn 91 7 extremely extremely RB cord-304685-s0bfhwtn 91 8 sensitive sensitive JJ cord-304685-s0bfhwtn 91 9 to to IN cord-304685-s0bfhwtn 91 10 apoptosis apoptosis NN cord-304685-s0bfhwtn 91 11 and and CC cord-304685-s0bfhwtn 91 12 consequently consequently RB cord-304685-s0bfhwtn 91 13 were be VBD cord-304685-s0bfhwtn 91 14 analyzed analyze VBN cord-304685-s0bfhwtn 91 15 in in IN cord-304685-s0bfhwtn 91 16 4 4 CD cord-304685-s0bfhwtn 91 17 - - SYM cord-304685-s0bfhwtn 91 18 6-h 6-h CD cord-304685-s0bfhwtn 91 19 cytotoxidty cytotoxidty NNP cord-304685-s0bfhwtn 91 20 assays assay NNS cord-304685-s0bfhwtn 91 21 . . . cord-304685-s0bfhwtn 92 1 Although although IN cord-304685-s0bfhwtn 92 2 the the DT cord-304685-s0bfhwtn 92 3 levd levd NN cord-304685-s0bfhwtn 92 4 of of IN cord-304685-s0bfhwtn 92 5 membrane membrane NN cord-304685-s0bfhwtn 92 6 lysis lysis NN cord-304685-s0bfhwtn 92 7 was be VBD cord-304685-s0bfhwtn 92 8 slightly slightly RB cord-304685-s0bfhwtn 92 9 greater great JJR cord-304685-s0bfhwtn 92 10 in in IN cord-304685-s0bfhwtn 92 11 A20 A20 NNP cord-304685-s0bfhwtn 92 12 cells cell NNS cord-304685-s0bfhwtn 92 13 than than IN cord-304685-s0bfhwtn 92 14 in in IN cord-304685-s0bfhwtn 92 15 P815 p815 PRP$ cord-304685-s0bfhwtn 92 16 ceils ceil NNS cord-304685-s0bfhwtn 93 1 ( ( -LRB- cord-304685-s0bfhwtn 93 2 Fig Fig NNP cord-304685-s0bfhwtn 93 3 . . . cord-304685-s0bfhwtn 93 4 3 3 CD cord-304685-s0bfhwtn 93 5 C C NNP cord-304685-s0bfhwtn 93 6 ) ) -RRB- cord-304685-s0bfhwtn 93 7 , , , cord-304685-s0bfhwtn 93 8 the the DT cord-304685-s0bfhwtn 93 9 level level NN cord-304685-s0bfhwtn 93 10 of of IN cord-304685-s0bfhwtn 93 11 DNA dna NN cord-304685-s0bfhwtn 93 12 fragmentation fragmentation NN cord-304685-s0bfhwtn 93 13 was be VBD cord-304685-s0bfhwtn 93 14 greater great JJR cord-304685-s0bfhwtn 93 15 in in IN cord-304685-s0bfhwtn 93 16 the the DT cord-304685-s0bfhwtn 93 17 faster fast RBR cord-304685-s0bfhwtn 93 18 growing grow VBG cord-304685-s0bfhwtn 93 19 P815 p815 NN cord-304685-s0bfhwtn 93 20 cells cell NNS cord-304685-s0bfhwtn 93 21 ( ( -LRB- cord-304685-s0bfhwtn 93 22 Fig fig NN cord-304685-s0bfhwtn 93 23 . . . cord-304685-s0bfhwtn 93 24 3/9 3/9 CD cord-304685-s0bfhwtn 93 25 ) ) -RRB- cord-304685-s0bfhwtn 93 26 . . . cord-304685-s0bfhwtn 94 1 Thus thus RB cord-304685-s0bfhwtn 94 2 , , , cord-304685-s0bfhwtn 94 3 P815 P815 '' cord-304685-s0bfhwtn 94 4 and and CC cord-304685-s0bfhwtn 94 5 A20 A20 NNP cord-304685-s0bfhwtn 94 6 cells cell NNS cord-304685-s0bfhwtn 94 7 underwent undergo VBD cord-304685-s0bfhwtn 94 8 apoptosis apoptosis NN cord-304685-s0bfhwtn 94 9 more more RBR cord-304685-s0bfhwtn 94 10 rapidly rapidly RB cord-304685-s0bfhwtn 94 11 than than IN cord-304685-s0bfhwtn 94 12 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 94 13 , , , cord-304685-s0bfhwtn 94 14 A31 A31 NNP cord-304685-s0bfhwtn 94 15 m m NNP cord-304685-s0bfhwtn 94 16 F F NNP cord-304685-s0bfhwtn 94 17 , , , cord-304685-s0bfhwtn 94 18 or or CC cord-304685-s0bfhwtn 94 19 L929 l929 CD cord-304685-s0bfhwtn 94 20 cells cell NNS cord-304685-s0bfhwtn 94 21 , , , cord-304685-s0bfhwtn 94 22 but but CC cord-304685-s0bfhwtn 94 23 in in IN cord-304685-s0bfhwtn 94 24 each each DT cord-304685-s0bfhwtn 94 25 case case NN cord-304685-s0bfhwtn 94 26 the the DT cord-304685-s0bfhwtn 94 27 faster fast RBR cord-304685-s0bfhwtn 94 28 growing grow VBG cord-304685-s0bfhwtn 94 29 cell cell NN cord-304685-s0bfhwtn 94 30 underwent undergo VBD cord-304685-s0bfhwtn 94 31 apoptosis apoptosis NN cord-304685-s0bfhwtn 94 32 more more RBR cord-304685-s0bfhwtn 94 33 readily readily RB cord-304685-s0bfhwtn 94 34 ( ( -LRB- cord-304685-s0bfhwtn 94 35 P815 p815 VB cord-304685-s0bfhwtn 94 36 > > XX cord-304685-s0bfhwtn 95 1 A20 A20 NNP cord-304685-s0bfhwtn 96 1 ; ; : cord-304685-s0bfhwtn 96 2 A31-myc A31-myc NNP cord-304685-s0bfhwtn 96 3 > > XX cord-304685-s0bfhwtn 96 4 L-929>3T3-A31 l-929>3t3-a31 NN cord-304685-s0bfhwtn 96 5 ) ) -RRB- cord-304685-s0bfhwtn 96 6 . . . cord-304685-s0bfhwtn 97 1 Demonstration demonstration NN cord-304685-s0bfhwtn 97 2 that that IN cord-304685-s0bfhwtn 97 3 each each DT cord-304685-s0bfhwtn 97 4 cell cell NN cord-304685-s0bfhwtn 97 5 line line NN cord-304685-s0bfhwtn 97 6 underwent undergo VBD cord-304685-s0bfhwtn 97 7 apoptosis apoptosis NN cord-304685-s0bfhwtn 97 8 associated associate VBN cord-304685-s0bfhwtn 97 9 with with IN cord-304685-s0bfhwtn 97 10 distinct distinct JJ cord-304685-s0bfhwtn 97 11 oligonucleosomal oligonucleosomal JJ cord-304685-s0bfhwtn 97 12 ladders ladder NNS cord-304685-s0bfhwtn 97 13 is be VBZ cord-304685-s0bfhwtn 97 14 presented present VBN cord-304685-s0bfhwtn 97 15 in in IN cord-304685-s0bfhwtn 97 16 Fig Fig NNP cord-304685-s0bfhwtn 97 17 . . NNP cord-304685-s0bfhwtn 97 18 4 4 CD cord-304685-s0bfhwtn 97 19 , , , cord-304685-s0bfhwtn 97 20 which which WDT cord-304685-s0bfhwtn 97 21 shows show VBZ cord-304685-s0bfhwtn 97 22 that that IN cord-304685-s0bfhwtn 97 23 the the DT cord-304685-s0bfhwtn 97 24 level level NN cord-304685-s0bfhwtn 97 25 of of IN cord-304685-s0bfhwtn 97 26 DNA dna NN cord-304685-s0bfhwtn 97 27 fragmentation fragmentation NN cord-304685-s0bfhwtn 97 28 in in IN cord-304685-s0bfhwtn 97 29 cells cell NNS cord-304685-s0bfhwtn 97 30 not not RB cord-304685-s0bfhwtn 97 31 exposed expose VBN cord-304685-s0bfhwtn 97 32 to to IN cord-304685-s0bfhwtn 97 33 CTL CTL NNP cord-304685-s0bfhwtn 97 34 ( ( -LRB- cord-304685-s0bfhwtn 97 35 C C NNP cord-304685-s0bfhwtn 97 36 ) ) -RRB- cord-304685-s0bfhwtn 97 37 , , , cord-304685-s0bfhwtn 97 38 and and CC cord-304685-s0bfhwtn 97 39 in in IN cord-304685-s0bfhwtn 97 40 the the DT cord-304685-s0bfhwtn 97 41 cell cell NN cord-304685-s0bfhwtn 97 42 pellets pellet NNS cord-304685-s0bfhwtn 97 43 ( ( -LRB- cord-304685-s0bfhwtn 97 44 P p NN cord-304685-s0bfhwtn 97 45 ) ) -RRB- cord-304685-s0bfhwtn 97 46 and and CC cord-304685-s0bfhwtn 97 47 culture culture NN cord-304685-s0bfhwtn 97 48 medium medium NN cord-304685-s0bfhwtn 97 49 ( ( -LRB- cord-304685-s0bfhwtn 97 50 M M NNP cord-304685-s0bfhwtn 97 51 ) ) -RRB- cord-304685-s0bfhwtn 97 52 from from IN cord-304685-s0bfhwtn 97 53 cells cell NNS cord-304685-s0bfhwtn 97 54 exposed expose VBN cord-304685-s0bfhwtn 97 55 to to IN cord-304685-s0bfhwtn 97 56 CTL CTL NNP cord-304685-s0bfhwtn 97 57 in in IN cord-304685-s0bfhwtn 97 58 6-(P815 6-(p815 CD cord-304685-s0bfhwtn 97 59 , , , cord-304685-s0bfhwtn 97 60 A20 A20 NNP cord-304685-s0bfhwtn 97 61 ) ) -RRB- cord-304685-s0bfhwtn 97 62 or or CC cord-304685-s0bfhwtn 97 63 10-(L929 10-(l929 CD cord-304685-s0bfhwtn 97 64 , , , cord-304685-s0bfhwtn 97 65 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 97 66 , , , cord-304685-s0bfhwtn 97 67 A31-mF A31-mF NNP cord-304685-s0bfhwtn 97 68 ) ) -RRB- cord-304685-s0bfhwtn 97 69 h h NN cord-304685-s0bfhwtn 97 70 assays assay NNS cord-304685-s0bfhwtn 97 71 . . . cord-304685-s0bfhwtn 98 1 Note note VB cord-304685-s0bfhwtn 98 2 both both CC cord-304685-s0bfhwtn 98 3 the the DT cord-304685-s0bfhwtn 98 4 presence presence NN cord-304685-s0bfhwtn 98 5 of of IN cord-304685-s0bfhwtn 98 6 the the DT cord-304685-s0bfhwtn 98 7 ladders ladder NNS cord-304685-s0bfhwtn 98 8 and and CC cord-304685-s0bfhwtn 98 9 the the DT cord-304685-s0bfhwtn 98 10 disappearance disappearance NN cord-304685-s0bfhwtn 98 11 of of IN cord-304685-s0bfhwtn 98 12 high high JJ cord-304685-s0bfhwtn 98 13 molecular molecular JJ cord-304685-s0bfhwtn 98 14 weight weight NN cord-304685-s0bfhwtn 98 15 DNA dna NN cord-304685-s0bfhwtn 98 16 , , , cord-304685-s0bfhwtn 98 17 the the DT cord-304685-s0bfhwtn 98 18 latter latter JJ cord-304685-s0bfhwtn 98 19 being be VBG cord-304685-s0bfhwtn 98 20 most most RBS cord-304685-s0bfhwtn 98 21 noticeable noticeable JJ cord-304685-s0bfhwtn 98 22 with with IN cord-304685-s0bfhwtn 98 23 the the DT cord-304685-s0bfhwtn 98 24 P815 P815 NNP cord-304685-s0bfhwtn 98 25 cells cell NNS cord-304685-s0bfhwtn 98 26 . . . cord-304685-s0bfhwtn 99 1 Pig pig NN cord-304685-s0bfhwtn 99 2 . . . cord-304685-s0bfhwtn 100 1 4 4 LS cord-304685-s0bfhwtn 100 2 also also RB cord-304685-s0bfhwtn 100 3 shows show VBZ cord-304685-s0bfhwtn 100 4 that that IN cord-304685-s0bfhwtn 100 5 the the DT cord-304685-s0bfhwtn 100 6 DNA dna NN cord-304685-s0bfhwtn 100 7 fragmentation fragmentation NN cord-304685-s0bfhwtn 100 8 in in IN cord-304685-s0bfhwtn 100 9 A31-myc A31-myc NNP cord-304685-s0bfhwtn 100 10 ceils ceils NNP cord-304685-s0bfhwtn 100 11 is be VBZ cord-304685-s0bfhwtn 100 12 far far RB cord-304685-s0bfhwtn 100 13 more more RBR cord-304685-s0bfhwtn 100 14 extensive extensive JJ cord-304685-s0bfhwtn 100 15 than than IN cord-304685-s0bfhwtn 100 16 in in IN cord-304685-s0bfhwtn 100 17 the the DT cord-304685-s0bfhwtn 100 18 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 100 19 cells cell NNS cord-304685-s0bfhwtn 100 20 . . . cord-304685-s0bfhwtn 101 1 Figttre Figttre NNP cord-304685-s0bfhwtn 101 2 4 4 CD cord-304685-s0bfhwtn 101 3 . . . cord-304685-s0bfhwtn 102 1 Target target VB cord-304685-s0bfhwtn 102 2 call call NN cord-304685-s0bfhwtn 102 3 DNA dna NN cord-304685-s0bfhwtn 102 4 is be VBZ cord-304685-s0bfhwtn 102 5 fragmented fragment VBN cord-304685-s0bfhwtn 102 6 into into IN cord-304685-s0bfhwtn 102 7 the the DT cord-304685-s0bfhwtn 102 8 nucleosomal nucleosomal JJ cord-304685-s0bfhwtn 102 9 ladder ladder NN cord-304685-s0bfhwtn 102 10 pattern pattern NN cord-304685-s0bfhwtn 102 11 typical typical JJ cord-304685-s0bfhwtn 102 12 of of IN cord-304685-s0bfhwtn 102 13 apoptosis apoptosis NN cord-304685-s0bfhwtn 102 14 . . . cord-304685-s0bfhwtn 103 1 Antoradiogram Antoradiogram NNP cord-304685-s0bfhwtn 103 2 of of IN cord-304685-s0bfhwtn 103 3 target target NN cord-304685-s0bfhwtn 103 4 cell cell NN cord-304685-s0bfhwtn 103 5 DNA dna NN cord-304685-s0bfhwtn 103 6 purified purify VBN cord-304685-s0bfhwtn 103 7 as as IN cord-304685-s0bfhwtn 103 8 described describe VBN cord-304685-s0bfhwtn 103 9 from from IN cord-304685-s0bfhwtn 103 10 either either CC cord-304685-s0bfhwtn 103 11 the the DT cord-304685-s0bfhwtn 103 12 assay assay NN cord-304685-s0bfhwtn 103 13 medium medium NN cord-304685-s0bfhwtn 103 14 ( ( -LRB- cord-304685-s0bfhwtn 103 15 214 214 CD cord-304685-s0bfhwtn 103 16 ) ) -RRB- cord-304685-s0bfhwtn 103 17 or or CC cord-304685-s0bfhwtn 103 18 from from IN cord-304685-s0bfhwtn 103 19 the the DT cord-304685-s0bfhwtn 103 20 cell cell NN cord-304685-s0bfhwtn 103 21 pellet pellet NN cord-304685-s0bfhwtn 103 22 ( ( -LRB- cord-304685-s0bfhwtn 103 23 P p NN cord-304685-s0bfhwtn 103 24 ) ) -RRB- cord-304685-s0bfhwtn 103 25 after after IN cord-304685-s0bfhwtn 103 26 the the DT cord-304685-s0bfhwtn 103 27 medium medium NN cord-304685-s0bfhwtn 103 28 had have VBD cord-304685-s0bfhwtn 103 29 been be VBN cord-304685-s0bfhwtn 103 30 removed remove VBN cord-304685-s0bfhwtn 103 31 or or CC cord-304685-s0bfhwtn 103 32 from from IN cord-304685-s0bfhwtn 103 33 target target NN cord-304685-s0bfhwtn 103 34 ceils ceil NNS cord-304685-s0bfhwtn 103 35 , , , cord-304685-s0bfhwtn 103 36 only only RB cord-304685-s0bfhwtn 103 37 , , , cord-304685-s0bfhwtn 103 38 without without IN cord-304685-s0bfhwtn 103 39 CTL CTL NNP cord-304685-s0bfhwtn 103 40 added add VBD cord-304685-s0bfhwtn 103 41 ( ( -LRB- cord-304685-s0bfhwtn 103 42 C C NNP cord-304685-s0bfhwtn 103 43 ) ) -RRB- cord-304685-s0bfhwtn 103 44 ( ( -LRB- cord-304685-s0bfhwtn 103 45 5 5 CD cord-304685-s0bfhwtn 103 46 ) ) -RRB- cord-304685-s0bfhwtn 103 47 . . . cord-304685-s0bfhwtn 104 1 Targets target NNS cord-304685-s0bfhwtn 104 2 were be VBD cord-304685-s0bfhwtn 104 3 exposed expose VBN cord-304685-s0bfhwtn 104 4 to to IN cord-304685-s0bfhwtn 104 5 CTL CTL NNP cord-304685-s0bfhwtn 104 6 for for IN cord-304685-s0bfhwtn 104 7 either either DT cord-304685-s0bfhwtn 104 8 6 6 CD cord-304685-s0bfhwtn 104 9 ( ( -LRB- cord-304685-s0bfhwtn 104 10 P815 P815 '' cord-304685-s0bfhwtn 104 11 , , , cord-304685-s0bfhwtn 104 12 A20 A20 NNP cord-304685-s0bfhwtn 104 13 ) ) -RRB- cord-304685-s0bfhwtn 104 14 or or CC cord-304685-s0bfhwtn 104 15 10 10 CD cord-304685-s0bfhwtn 104 16 h h NN cord-304685-s0bfhwtn 104 17 ( ( -LRB- cord-304685-s0bfhwtn 104 18 b929 b929 NNP cord-304685-s0bfhwtn 104 19 , , , cord-304685-s0bfhwtn 104 20 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 104 21 , , , cord-304685-s0bfhwtn 104 22 A31-ngr A31-ngr NNP cord-304685-s0bfhwtn 104 23 ) ) -RRB- cord-304685-s0bfhwtn 104 24 assays assay NNS cord-304685-s0bfhwtn 104 25 . . . cord-304685-s0bfhwtn 105 1 DNA dna NN cord-304685-s0bfhwtn 105 2 fragmentation fragmentation NN cord-304685-s0bfhwtn 105 3 was be VBD cord-304685-s0bfhwtn 105 4 assessed assess VBN cord-304685-s0bfhwtn 105 5 from from IN cord-304685-s0bfhwtn 105 6 samples sample NNS cord-304685-s0bfhwtn 105 7 at at IN cord-304685-s0bfhwtn 105 8 an an DT cord-304685-s0bfhwtn 105 9 E e NN cord-304685-s0bfhwtn 105 10 / / SYM cord-304685-s0bfhwtn 105 11 T t NN cord-304685-s0bfhwtn 105 12 ratio ratio NN cord-304685-s0bfhwtn 105 13 of of IN cord-304685-s0bfhwtn 105 14 50:1 50:1 CD cord-304685-s0bfhwtn 105 15 . . . cord-304685-s0bfhwtn 106 1 Control control NN cord-304685-s0bfhwtn 106 2 samples sample NNS cord-304685-s0bfhwtn 106 3 were be VBD cord-304685-s0bfhwtn 106 4 from from IN cord-304685-s0bfhwtn 106 5 targets target NNS cord-304685-s0bfhwtn 106 6 without without IN cord-304685-s0bfhwtn 106 7 director director NN cord-304685-s0bfhwtn 106 8 cells cell NNS cord-304685-s0bfhwtn 106 9 . . . cord-304685-s0bfhwtn 107 1 30,000 30,000 CD cord-304685-s0bfhwtn 107 2 cpm cpm NNS cord-304685-s0bfhwtn 107 3 + + SYM cord-304685-s0bfhwtn 107 4 10 10 CD cord-304685-s0bfhwtn 107 5 % % NN cord-304685-s0bfhwtn 107 6 of of IN cord-304685-s0bfhwtn 107 7 each each DT cord-304685-s0bfhwtn 107 8 sample sample NN cord-304685-s0bfhwtn 107 9 component component NN cord-304685-s0bfhwtn 107 10 was be VBD cord-304685-s0bfhwtn 107 11 loaded load VBN cord-304685-s0bfhwtn 107 12 onto onto IN cord-304685-s0bfhwtn 107 13 1.5 1.5 CD cord-304685-s0bfhwtn 107 14 % % NN cord-304685-s0bfhwtn 107 15 agarose agarose JJ cord-304685-s0bfhwtn 107 16 gels gel NNS cord-304685-s0bfhwtn 107 17 . . . cord-304685-s0bfhwtn 108 1 Gels gel NNS cord-304685-s0bfhwtn 108 2 were be VBD cord-304685-s0bfhwtn 108 3 dried dry VBN cord-304685-s0bfhwtn 108 4 and and CC cord-304685-s0bfhwtn 108 5 exposed expose VBN cord-304685-s0bfhwtn 108 6 at at IN cord-304685-s0bfhwtn 108 7 -80oc -80oc NN cord-304685-s0bfhwtn 108 8 with with IN cord-304685-s0bfhwtn 108 9 two two CD cord-304685-s0bfhwtn 108 10 screens screen NNS cord-304685-s0bfhwtn 108 11 ( ( -LRB- cord-304685-s0bfhwtn 108 12 5 5 CD cord-304685-s0bfhwtn 108 13 ) ) -RRB- cord-304685-s0bfhwtn 108 14 . . . cord-304685-s0bfhwtn 109 1 Many many JJ cord-304685-s0bfhwtn 109 2 DNA dna NN cord-304685-s0bfhwtn 109 3 viruses virus NNS cord-304685-s0bfhwtn 109 4 induce induce VBP cord-304685-s0bfhwtn 109 5 quiescent quiescent JJ cord-304685-s0bfhwtn 109 6 cells cell NNS cord-304685-s0bfhwtn 109 7 to to TO cord-304685-s0bfhwtn 109 8 progress progress VB cord-304685-s0bfhwtn 109 9 into into IN cord-304685-s0bfhwtn 109 10 a a DT cord-304685-s0bfhwtn 109 11 late late JJ cord-304685-s0bfhwtn 109 12 G1 G1 NNP cord-304685-s0bfhwtn 109 13 / / SYM cord-304685-s0bfhwtn 109 14 S S NNP cord-304685-s0bfhwtn 109 15 - - HYPH cord-304685-s0bfhwtn 109 16 like like JJ cord-304685-s0bfhwtn 109 17 phase phase NN cord-304685-s0bfhwtn 109 18 to to TO cord-304685-s0bfhwtn 109 19 obtain obtain VB cord-304685-s0bfhwtn 109 20 cell cell NN cord-304685-s0bfhwtn 109 21 - - HYPH cord-304685-s0bfhwtn 109 22 regulated regulate VBN cord-304685-s0bfhwtn 109 23 components component NNS cord-304685-s0bfhwtn 109 24 of of IN cord-304685-s0bfhwtn 109 25 the the DT cord-304685-s0bfhwtn 109 26 cell cell NN cord-304685-s0bfhwtn 109 27 's 's POS cord-304685-s0bfhwtn 109 28 DNA dna NN cord-304685-s0bfhwtn 109 29 synthetic synthetic JJ cord-304685-s0bfhwtn 109 30 machinery machinery NN cord-304685-s0bfhwtn 109 31 ( ( -LRB- cord-304685-s0bfhwtn 109 32 18 18 CD cord-304685-s0bfhwtn 109 33 ) ) -RRB- cord-304685-s0bfhwtn 109 34 ( ( -LRB- cord-304685-s0bfhwtn 109 35 19 19 CD cord-304685-s0bfhwtn 109 36 ) ) -RRB- cord-304685-s0bfhwtn 110 1 ( ( -LRB- cord-304685-s0bfhwtn 110 2 20 20 CD cord-304685-s0bfhwtn 110 3 ) ) -RRB- cord-304685-s0bfhwtn 110 4 ( ( -LRB- cord-304685-s0bfhwtn 110 5 21 21 CD cord-304685-s0bfhwtn 110 6 ) ) -RRB- cord-304685-s0bfhwtn 110 7 . . . cord-304685-s0bfhwtn 111 1 The the DT cord-304685-s0bfhwtn 111 2 mechanism mechanism NN cord-304685-s0bfhwtn 111 3 of of IN cord-304685-s0bfhwtn 111 4 induction induction NN cord-304685-s0bfhwtn 111 5 into into IN cord-304685-s0bfhwtn 111 6 the the DT cord-304685-s0bfhwtn 111 7 DNA dna NN cord-304685-s0bfhwtn 111 8 synthetic synthetic JJ cord-304685-s0bfhwtn 111 9 state state NN cord-304685-s0bfhwtn 111 10 by by IN cord-304685-s0bfhwtn 111 11 DNA dna NN cord-304685-s0bfhwtn 111 12 viruses virus NNS cord-304685-s0bfhwtn 111 13 differ differ VBP cord-304685-s0bfhwtn 111 14 , , , cord-304685-s0bfhwtn 111 15 but but CC cord-304685-s0bfhwtn 111 16 the the DT cord-304685-s0bfhwtn 111 17 effects effect NNS cord-304685-s0bfhwtn 111 18 are be VBP cord-304685-s0bfhwtn 111 19 the the DT cord-304685-s0bfhwtn 111 20 same same JJ cord-304685-s0bfhwtn 111 21 , , , cord-304685-s0bfhwtn 111 22 i.e. i.e. FW cord-304685-s0bfhwtn 111 23 , , , cord-304685-s0bfhwtn 111 24 activation activation NN cord-304685-s0bfhwtn 111 25 of of IN cord-304685-s0bfhwtn 111 26 the the DT cord-304685-s0bfhwtn 111 27 competence competence NN cord-304685-s0bfhwtn 111 28 mechanism mechanism NN cord-304685-s0bfhwtn 111 29 for for IN cord-304685-s0bfhwtn 111 30 cell cell NN cord-304685-s0bfhwtn 111 31 cycle cycle NN cord-304685-s0bfhwtn 111 32 progression progression NN cord-304685-s0bfhwtn 111 33 and and CC cord-304685-s0bfhwtn 111 34 the the DT cord-304685-s0bfhwtn 111 35 production production NN cord-304685-s0bfhwtn 111 36 of of IN cord-304685-s0bfhwtn 111 37 enzymes enzyme NNS cord-304685-s0bfhwtn 111 38 needed need VBN cord-304685-s0bfhwtn 111 39 for for IN cord-304685-s0bfhwtn 111 40 DNA dna NN cord-304685-s0bfhwtn 111 41 synthesis synthesis NN cord-304685-s0bfhwtn 111 42 even even RB cord-304685-s0bfhwtn 111 43 if if IN cord-304685-s0bfhwtn 111 44 the the DT cord-304685-s0bfhwtn 111 45 DNA dna NN cord-304685-s0bfhwtn 111 46 synthesized synthesize VBN cord-304685-s0bfhwtn 111 47 is be VBZ cord-304685-s0bfhwtn 111 48 preferentially preferentially RB cord-304685-s0bfhwtn 111 49 viral viral JJ cord-304685-s0bfhwtn 111 50 ( ( -LRB- cord-304685-s0bfhwtn 111 51 18 18 CD cord-304685-s0bfhwtn 111 52 ) ) -RRB- cord-304685-s0bfhwtn 111 53 ( ( -LRB- cord-304685-s0bfhwtn 111 54 19 19 CD cord-304685-s0bfhwtn 111 55 ) ) -RRB- cord-304685-s0bfhwtn 111 56 ( ( -LRB- cord-304685-s0bfhwtn 111 57 20 20 CD cord-304685-s0bfhwtn 111 58 ) ) -RRB- cord-304685-s0bfhwtn 111 59 ( ( -LRB- cord-304685-s0bfhwtn 111 60 21 21 CD cord-304685-s0bfhwtn 111 61 ) ) -RRB- cord-304685-s0bfhwtn 111 62 . . . cord-304685-s0bfhwtn 112 1 Numerous numerous JJ cord-304685-s0bfhwtn 112 2 herpes herpes NN cord-304685-s0bfhwtn 112 3 viruses virus NNS cord-304685-s0bfhwtn 112 4 induce induce VBP cord-304685-s0bfhwtn 112 5 the the DT cord-304685-s0bfhwtn 112 6 activation activation NN cord-304685-s0bfhwtn 112 7 of of IN cord-304685-s0bfhwtn 112 8 quiescent quiescent JJ cord-304685-s0bfhwtn 112 9 cells cell NNS cord-304685-s0bfhwtn 112 10 into into IN cord-304685-s0bfhwtn 112 11 the the DT cord-304685-s0bfhwtn 112 12 DNA dna NN cord-304685-s0bfhwtn 112 13 synthetic synthetic NN cord-304685-s0bfhwtn 112 14 phase phase NN cord-304685-s0bfhwtn 112 15 and and CC cord-304685-s0bfhwtn 112 16 upregulate upregulate VB cord-304685-s0bfhwtn 112 17 the the DT cord-304685-s0bfhwtn 112 18 activity activity NN cord-304685-s0bfhwtn 112 19 of of IN cord-304685-s0bfhwtn 112 20 DNA dna NN cord-304685-s0bfhwtn 112 21 topoisomerases topoisomerase NNS cord-304685-s0bfhwtn 112 22 I -PRON- PRP cord-304685-s0bfhwtn 112 23 and/or and/or CC cord-304685-s0bfhwtn 112 24 II II NNP cord-304685-s0bfhwtn 112 25 , , , cord-304685-s0bfhwtn 112 26 which which WDT cord-304685-s0bfhwtn 112 27 the the DT cord-304685-s0bfhwtn 112 28 viruses virus NNS cord-304685-s0bfhwtn 112 29 require require VBP cord-304685-s0bfhwtn 112 30 for for IN cord-304685-s0bfhwtn 112 31 replication replication NN cord-304685-s0bfhwtn 112 32 ( ( -LRB- cord-304685-s0bfhwtn 112 33 20 20 CD cord-304685-s0bfhwtn 112 34 , , , cord-304685-s0bfhwtn 112 35 21 21 CD cord-304685-s0bfhwtn 112 36 ) ) -RRB- cord-304685-s0bfhwtn 112 37 . . . cord-304685-s0bfhwtn 113 1 When when WRB cord-304685-s0bfhwtn 113 2 quiescent quiescent JJ cord-304685-s0bfhwtn 113 3 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 113 4 cells cell NNS cord-304685-s0bfhwtn 113 5 were be VBD cord-304685-s0bfhwtn 113 6 infected infect VBN cord-304685-s0bfhwtn 113 7 with with IN cord-304685-s0bfhwtn 113 8 HSV-1 HSV-1 NNS cord-304685-s0bfhwtn 113 9 , , , cord-304685-s0bfhwtn 113 10 these these DT cord-304685-s0bfhwtn 113 11 cells cell NNS cord-304685-s0bfhwtn 113 12 became become VBD cord-304685-s0bfhwtn 113 13 more more RBR cord-304685-s0bfhwtn 113 14 susceptible susceptible JJ cord-304685-s0bfhwtn 113 15 to to IN cord-304685-s0bfhwtn 113 16 CTLinduced ctlinduce VBN cord-304685-s0bfhwtn 113 17 DNA dna NN cord-304685-s0bfhwtn 113 18 fragmentation fragmentation NN cord-304685-s0bfhwtn 113 19 than than IN cord-304685-s0bfhwtn 113 20 3T3-A31 3t3-a31 JJ cord-304685-s0bfhwtn 113 21 ceils ceil NNS cord-304685-s0bfhwtn 113 22 in in IN cord-304685-s0bfhwtn 113 23 log log NN cord-304685-s0bfhwtn 113 24 phase phase NN cord-304685-s0bfhwtn 113 25 growth growth NN cord-304685-s0bfhwtn 113 26 and and CC cord-304685-s0bfhwtn 113 27 markedly markedly RB cord-304685-s0bfhwtn 113 28 more more RBR cord-304685-s0bfhwtn 113 29 susceptible susceptible JJ cord-304685-s0bfhwtn 113 30 than than IN cord-304685-s0bfhwtn 113 31 their -PRON- PRP$ cord-304685-s0bfhwtn 113 32 quiescent quiescent JJ cord-304685-s0bfhwtn 113 33 counterparts counterpart NNS cord-304685-s0bfhwtn 113 34 ( ( -LRB- cord-304685-s0bfhwtn 113 35 Fig fig NN cord-304685-s0bfhwtn 113 36 . . . cord-304685-s0bfhwtn 114 1 5 5 LS cord-304685-s0bfhwtn 114 2 , , , cord-304685-s0bfhwtn 114 3 A a NN cord-304685-s0bfhwtn 114 4 and and CC cord-304685-s0bfhwtn 114 5 B b NN cord-304685-s0bfhwtn 114 6 ) ) -RRB- cord-304685-s0bfhwtn 114 7 . . . cord-304685-s0bfhwtn 115 1 The the DT cord-304685-s0bfhwtn 115 2 level level NN cord-304685-s0bfhwtn 115 3 of of IN cord-304685-s0bfhwtn 115 4 CTL ctl NN cord-304685-s0bfhwtn 115 5 - - HYPH cord-304685-s0bfhwtn 115 6 induced induce VBN cord-304685-s0bfhwtn 115 7 membrane membrane NN cord-304685-s0bfhwtn 115 8 lysis lysis NN cord-304685-s0bfhwtn 115 9 was be VBD cord-304685-s0bfhwtn 115 10 not not RB cord-304685-s0bfhwtn 115 11 significantly significantly RB cord-304685-s0bfhwtn 115 12 different different JJ cord-304685-s0bfhwtn 115 13 between between IN cord-304685-s0bfhwtn 115 14 the the DT cord-304685-s0bfhwtn 115 15 three three CD cord-304685-s0bfhwtn 115 16 groups group NNS cord-304685-s0bfhwtn 115 17 ( ( -LRB- cord-304685-s0bfhwtn 115 18 Fig Fig NNP cord-304685-s0bfhwtn 115 19 . . . cord-304685-s0bfhwtn 115 20 5 5 CD cord-304685-s0bfhwtn 115 21 A a NN cord-304685-s0bfhwtn 115 22 ) ) -RRB- cord-304685-s0bfhwtn 115 23 . . . cord-304685-s0bfhwtn 116 1 A a DT cord-304685-s0bfhwtn 116 2 previous previous JJ cord-304685-s0bfhwtn 116 3 study study NN cord-304685-s0bfhwtn 116 4 ( ( -LRB- cord-304685-s0bfhwtn 116 5 22 22 CD cord-304685-s0bfhwtn 116 6 ) ) -RRB- cord-304685-s0bfhwtn 116 7 suggested suggest VBD cord-304685-s0bfhwtn 116 8 that that IN cord-304685-s0bfhwtn 116 9 HSV HSV NNP cord-304685-s0bfhwtn 116 10 - - HYPH cord-304685-s0bfhwtn 116 11 l l NN cord-304685-s0bfhwtn 116 12 - - HYPH cord-304685-s0bfhwtn 116 13 infected infect VBN cord-304685-s0bfhwtn 116 14 cells cell NNS cord-304685-s0bfhwtn 116 15 were be VBD cord-304685-s0bfhwtn 116 16 highly highly RB cord-304685-s0bfhwtn 116 17 sensitive sensitive JJ cord-304685-s0bfhwtn 116 18 to to IN cord-304685-s0bfhwtn 116 19 killing kill VBG cord-304685-s0bfhwtn 116 20 by by IN cord-304685-s0bfhwtn 116 21 TNF TNF NNP cord-304685-s0bfhwtn 116 22 - - HYPH cord-304685-s0bfhwtn 116 23 ct ct NNP cord-304685-s0bfhwtn 116 24 , , , cord-304685-s0bfhwtn 116 25 but but CC cord-304685-s0bfhwtn 116 26 inclusion inclusion NN cord-304685-s0bfhwtn 116 27 of of IN cord-304685-s0bfhwtn 116 28 anti anti JJ cord-304685-s0bfhwtn 116 29 - - JJ cord-304685-s0bfhwtn 116 30 TNF TNF NNP cord-304685-s0bfhwtn 116 31 antisera antisera NN cord-304685-s0bfhwtn 116 32 in in IN cord-304685-s0bfhwtn 116 33 our -PRON- PRP$ cord-304685-s0bfhwtn 116 34 assays assay NNS cord-304685-s0bfhwtn 116 35 did do VBD cord-304685-s0bfhwtn 116 36 not not RB cord-304685-s0bfhwtn 116 37 block block VB cord-304685-s0bfhwtn 116 38 the the DT cord-304685-s0bfhwtn 116 39 DNA dna NN cord-304685-s0bfhwtn 116 40 fragmentation fragmentation NN cord-304685-s0bfhwtn 116 41 nor nor CC cord-304685-s0bfhwtn 116 42 the the DT cord-304685-s0bfhwtn 116 43 membrane membrane NN cord-304685-s0bfhwtn 116 44 lysis lysis NN cord-304685-s0bfhwtn 116 45 of of IN cord-304685-s0bfhwtn 116 46 the the DT cord-304685-s0bfhwtn 116 47 target target NN cord-304685-s0bfhwtn 116 48 cells cell NNS cord-304685-s0bfhwtn 116 49 . . . cord-304685-s0bfhwtn 117 1 Pulse pulse NN cord-304685-s0bfhwtn 117 2 - - HYPH cord-304685-s0bfhwtn 117 3 labeling labeling NN cord-304685-s0bfhwtn 117 4 studies study NNS cord-304685-s0bfhwtn 117 5 of of IN cord-304685-s0bfhwtn 117 6 the the DT cord-304685-s0bfhwtn 117 7 HSV HSV NNP cord-304685-s0bfhwtn 117 8 - - HYPH cord-304685-s0bfhwtn 117 9 infected infect VBN cord-304685-s0bfhwtn 117 10 quiescent quiescent JJ cord-304685-s0bfhwtn 117 11 vs. vs. FW cord-304685-s0bfhwtn 117 12 uninfected uninfected JJ cord-304685-s0bfhwtn 117 13 quiescent quiescent JJ cord-304685-s0bfhwtn 117 14 targets target NNS cord-304685-s0bfhwtn 117 15 showed show VBD cord-304685-s0bfhwtn 117 16 , , , cord-304685-s0bfhwtn 117 17 as as IN cord-304685-s0bfhwtn 117 18 expected expect VBN cord-304685-s0bfhwtn 117 19 , , , cord-304685-s0bfhwtn 117 20 that that DT cord-304685-s0bfhwtn 117 21 DNA dna NN cord-304685-s0bfhwtn 117 22 synthesis synthesis NN cord-304685-s0bfhwtn 117 23 was be VBD cord-304685-s0bfhwtn 117 24 initiated initiate VBN cord-304685-s0bfhwtn 117 25 in in IN cord-304685-s0bfhwtn 117 26 the the DT cord-304685-s0bfhwtn 117 27 HSV HSV NNP cord-304685-s0bfhwtn 117 28 - - HYPH cord-304685-s0bfhwtn 117 29 infected infect VBN cord-304685-s0bfhwtn 117 30 quiescent quiescent JJ cord-304685-s0bfhwtn 117 31 cells cell NNS cord-304685-s0bfhwtn 117 32 and and CC cord-304685-s0bfhwtn 117 33 remained remain VBD cord-304685-s0bfhwtn 117 34 high high JJ cord-304685-s0bfhwtn 117 35 throughout throughout IN cord-304685-s0bfhwtn 117 36 the the DT cord-304685-s0bfhwtn 117 37 time time NN cord-304685-s0bfhwtn 117 38 of of IN cord-304685-s0bfhwtn 117 39 assay assay NN cord-304685-s0bfhwtn 117 40 ( ( -LRB- cord-304685-s0bfhwtn 117 41 data datum NNS cord-304685-s0bfhwtn 117 42 not not RB cord-304685-s0bfhwtn 117 43 shown show VBN cord-304685-s0bfhwtn 117 44 ) ) -RRB- cord-304685-s0bfhwtn 117 45 . . . cord-304685-s0bfhwtn 118 1 This this DT cord-304685-s0bfhwtn 118 2 DNA dna NN cord-304685-s0bfhwtn 118 3 synthesis synthesis NN cord-304685-s0bfhwtn 118 4 was be VBD cord-304685-s0bfhwtn 118 5 probably probably RB cord-304685-s0bfhwtn 118 6 viral viral JJ cord-304685-s0bfhwtn 118 7 , , , cord-304685-s0bfhwtn 118 8 as as IN cord-304685-s0bfhwtn 118 9 HSV-1 hsv-1 NN cord-304685-s0bfhwtn 118 10 infection infection NN cord-304685-s0bfhwtn 118 11 inhibits inhibit VBZ cord-304685-s0bfhwtn 118 12 cellular cellular JJ cord-304685-s0bfhwtn 118 13 DNA dna NN cord-304685-s0bfhwtn 118 14 synthesis synthesis NN cord-304685-s0bfhwtn 118 15 while while IN cord-304685-s0bfhwtn 118 16 stimulating stimulate VBG cord-304685-s0bfhwtn 118 17 cellular cellular JJ cord-304685-s0bfhwtn 118 18 DNA dna NN cord-304685-s0bfhwtn 118 19 synthetic synthetic NN cord-304685-s0bfhwtn 118 20 enzymes enzyme NNS cord-304685-s0bfhwtn 118 21 ( ( -LRB- cord-304685-s0bfhwtn 118 22 23 23 CD cord-304685-s0bfhwtn 118 23 ) ) -RRB- cord-304685-s0bfhwtn 118 24 . . . cord-304685-s0bfhwtn 119 1 Thus thus RB cord-304685-s0bfhwtn 119 2 , , , cord-304685-s0bfhwtn 119 3 HSV-1 hsv-1 NN cord-304685-s0bfhwtn 119 4 infection infection NN cord-304685-s0bfhwtn 119 5 of of IN cord-304685-s0bfhwtn 119 6 the the DT cord-304685-s0bfhwtn 119 7 target target NN cord-304685-s0bfhwtn 119 8 cell cell NN cord-304685-s0bfhwtn 119 9 induced induce VBD cord-304685-s0bfhwtn 119 10 a a DT cord-304685-s0bfhwtn 119 11 state state NN cord-304685-s0bfhwtn 119 12 of of IN cord-304685-s0bfhwtn 119 13 competence competence NN cord-304685-s0bfhwtn 119 14 for for IN cord-304685-s0bfhwtn 119 15 extreme extreme JJ cord-304685-s0bfhwtn 119 16 susceptibility susceptibility NN cord-304685-s0bfhwtn 119 17 to to IN cord-304685-s0bfhwtn 119 18 CTL CTL NNP cord-304685-s0bfhwtn 119 19 - - HYPH cord-304685-s0bfhwtn 119 20 induced induce VBN cord-304685-s0bfhwtn 119 21 apoptosis apoptosis NN cord-304685-s0bfhwtn 119 22 . . . cord-304685-s0bfhwtn 120 1 This this DT cord-304685-s0bfhwtn 120 2 virus virus NN cord-304685-s0bfhwtn 120 3 - - HYPH cord-304685-s0bfhwtn 120 4 induced induce VBN cord-304685-s0bfhwtn 120 5 competence competence NN cord-304685-s0bfhwtn 120 6 was be VBD cord-304685-s0bfhwtn 120 7 not not RB cord-304685-s0bfhwtn 120 8 due due JJ cord-304685-s0bfhwtn 120 9 to to IN cord-304685-s0bfhwtn 120 10 serum serum NN cord-304685-s0bfhwtn 120 11 con- con- NN cord-304685-s0bfhwtn 120 12 Figure figure NN cord-304685-s0bfhwtn 120 13 5 5 CD cord-304685-s0bfhwtn 120 14 . . . cord-304685-s0bfhwtn 121 1 Infection infection NN cord-304685-s0bfhwtn 121 2 of of IN cord-304685-s0bfhwtn 121 3 quiescent quiescent JJ cord-304685-s0bfhwtn 121 4 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 121 5 cells cell NNS cord-304685-s0bfhwtn 121 6 with with IN cord-304685-s0bfhwtn 121 7 HSV-1 HSV-1 NNS cord-304685-s0bfhwtn 121 8 renders render VBZ cord-304685-s0bfhwtn 121 9 these these DT cord-304685-s0bfhwtn 121 10 targets target NNS cord-304685-s0bfhwtn 121 11 , , , cord-304685-s0bfhwtn 121 12 otherwise otherwise RB cord-304685-s0bfhwtn 121 13 refractory refractory JJ cord-304685-s0bfhwtn 121 14 to to IN cord-304685-s0bfhwtn 121 15 apoptosis apoptosis NN cord-304685-s0bfhwtn 121 16 , , , cord-304685-s0bfhwtn 121 17 highly highly RB cord-304685-s0bfhwtn 121 18 susceptible susceptible JJ cord-304685-s0bfhwtn 121 19 to to IN cord-304685-s0bfhwtn 121 20 CTbinduced ctbinduced JJ cord-304685-s0bfhwtn 121 21 apoptosis apoptosis NN cord-304685-s0bfhwtn 121 22 . . . cord-304685-s0bfhwtn 122 1 ( ( -LRB- cord-304685-s0bfhwtn 122 2 A a NN cord-304685-s0bfhwtn 122 3 ) ) -RRB- cord-304685-s0bfhwtn 122 4 Lysis lysis NN cord-304685-s0bfhwtn 122 5 of of IN cord-304685-s0bfhwtn 122 6 targets target NNS cord-304685-s0bfhwtn 122 7 either either CC cord-304685-s0bfhwtn 122 8 in in IN cord-304685-s0bfhwtn 122 9 log log NN cord-304685-s0bfhwtn 122 10 phase phase NN cord-304685-s0bfhwtn 122 11 growth growth NN cord-304685-s0bfhwtn 122 12 , , , cord-304685-s0bfhwtn 122 13 quiescent quiescent JJ cord-304685-s0bfhwtn 122 14 , , , cord-304685-s0bfhwtn 122 15 or or CC cord-304685-s0bfhwtn 122 16 quiescent+HSVol quiescent+hsvol ADD cord-304685-s0bfhwtn 122 17 infection infection NN cord-304685-s0bfhwtn 122 18 . . . cord-304685-s0bfhwtn 123 1 ( ( -LRB- cord-304685-s0bfhwtn 123 2 B b NN cord-304685-s0bfhwtn 123 3 ) ) -RRB- cord-304685-s0bfhwtn 123 4 Sohbilized Sohbilized NNP cord-304685-s0bfhwtn 123 5 lZq lzq NN cord-304685-s0bfhwtn 123 6 - - HYPH cord-304685-s0bfhwtn 123 7 DNA dna NN cord-304685-s0bfhwtn 123 8 rdease rdease NN cord-304685-s0bfhwtn 123 9 from from IN cord-304685-s0bfhwtn 123 10 targets target NNS cord-304685-s0bfhwtn 123 11 as as IN cord-304685-s0bfhwtn 123 12 in in IN cord-304685-s0bfhwtn 123 13 A. a. NN cord-304685-s0bfhwtn 123 14 Quiescent quiescent JJ cord-304685-s0bfhwtn 123 15 targets target NNS cord-304685-s0bfhwtn 123 16 and and CC cord-304685-s0bfhwtn 123 17 targets target NNS cord-304685-s0bfhwtn 123 18 in in IN cord-304685-s0bfhwtn 123 19 log log NN cord-304685-s0bfhwtn 123 20 phase phase NN cord-304685-s0bfhwtn 123 21 growth growth NN cord-304685-s0bfhwtn 123 22 were be VBD cord-304685-s0bfhwtn 123 23 prepared prepare VBN cord-304685-s0bfhwtn 123 24 as as IN cord-304685-s0bfhwtn 123 25 in in IN cord-304685-s0bfhwtn 123 26 Hg Hg NNP cord-304685-s0bfhwtn 123 27 . . . cord-304685-s0bfhwtn 124 1 2.5 2.5 CD cord-304685-s0bfhwtn 124 2 d d NNS cord-304685-s0bfhwtn 124 3 before before IN cord-304685-s0bfhwtn 124 4 use use NN cord-304685-s0bfhwtn 124 5 ( ( -LRB- cord-304685-s0bfhwtn 124 6 day day NN cord-304685-s0bfhwtn 124 7 -5 -5 NNP cord-304685-s0bfhwtn 124 8 ) ) -RRB- cord-304685-s0bfhwtn 124 9 , , , cord-304685-s0bfhwtn 124 10 3T3-A31 3t3-a31 CD cord-304685-s0bfhwtn 124 11 cells cell NNS cord-304685-s0bfhwtn 124 12 were be VBD cord-304685-s0bfhwtn 124 13 subcultured subculture VBN cord-304685-s0bfhwtn 124 14 into into IN cord-304685-s0bfhwtn 124 15 T150 t150 NN cord-304685-s0bfhwtn 124 16 era era NN cord-304685-s0bfhwtn 124 17 2 2 CD cord-304685-s0bfhwtn 124 18 flasks flask NNS cord-304685-s0bfhwtn 124 19 to to TO cord-304685-s0bfhwtn 124 20 obtain obtain VB cord-304685-s0bfhwtn 124 21 ~75 ~75 NNP cord-304685-s0bfhwtn 124 22 % % NN cord-304685-s0bfhwtn 124 23 confluency confluency NN cord-304685-s0bfhwtn 124 24 . . . cord-304685-s0bfhwtn 125 1 On on IN cord-304685-s0bfhwtn 125 2 day day NN cord-304685-s0bfhwtn 125 3 -4 -4 NNP cord-304685-s0bfhwtn 125 4 , , , cord-304685-s0bfhwtn 125 5 they -PRON- PRP cord-304685-s0bfhwtn 125 6 were be VBD cord-304685-s0bfhwtn 125 7 labeled label VBN cord-304685-s0bfhwtn 125 8 with with IN cord-304685-s0bfhwtn 125 9 1zsI 1zsi CD cord-304685-s0bfhwtn 125 10 - - HYPH cord-304685-s0bfhwtn 125 11 IUDR IUDR NNP cord-304685-s0bfhwtn 125 12 at at IN cord-304685-s0bfhwtn 125 13 1/~Ci 1/~ci CD cord-304685-s0bfhwtn 125 14 / / SYM cord-304685-s0bfhwtn 125 15 ml ml NN cord-304685-s0bfhwtn 125 16 in in IN cord-304685-s0bfhwtn 125 17 a a DT cord-304685-s0bfhwtn 125 18 total total JJ cord-304685-s0bfhwtn 125 19 volume volume NN cord-304685-s0bfhwtn 125 20 of of IN cord-304685-s0bfhwtn 125 21 10 10 CD cord-304685-s0bfhwtn 125 22 ml ml NNS cord-304685-s0bfhwtn 125 23 for for IN cord-304685-s0bfhwtn 125 24 18 18 CD cord-304685-s0bfhwtn 125 25 h. h. NN cord-304685-s0bfhwtn 126 1 On on IN cord-304685-s0bfhwtn 126 2 day day NN cord-304685-s0bfhwtn 126 3 -3 -3 NNP cord-304685-s0bfhwtn 126 4 the the DT cord-304685-s0bfhwtn 126 5 medium medium NN cord-304685-s0bfhwtn 126 6 was be VBD cord-304685-s0bfhwtn 126 7 removed remove VBN cord-304685-s0bfhwtn 126 8 , , , cord-304685-s0bfhwtn 126 9 fresh fresh JJ cord-304685-s0bfhwtn 126 10 RPMI RPMI NNP cord-304685-s0bfhwtn 126 11 + + CC cord-304685-s0bfhwtn 127 1 5 5 CD cord-304685-s0bfhwtn 127 2 % % NN cord-304685-s0bfhwtn 127 3 CPSK-2 CPSK-2 NNP cord-304685-s0bfhwtn 127 4 was be VBD cord-304685-s0bfhwtn 127 5 added add VBN cord-304685-s0bfhwtn 127 6 , , , cord-304685-s0bfhwtn 127 7 and and CC cord-304685-s0bfhwtn 127 8 the the DT cord-304685-s0bfhwtn 127 9 cells cell NNS cord-304685-s0bfhwtn 127 10 were be VBD cord-304685-s0bfhwtn 127 11 allowed allow VBN cord-304685-s0bfhwtn 127 12 to to TO cord-304685-s0bfhwtn 127 13 quiesce quiesce VB cord-304685-s0bfhwtn 127 14 for for IN cord-304685-s0bfhwtn 127 15 72 72 CD cord-304685-s0bfhwtn 127 16 h. h. NN cord-304685-s0bfhwtn 128 1 For for IN cord-304685-s0bfhwtn 128 2 HSV-1 hsv-1 NN cord-304685-s0bfhwtn 128 3 inf inf NNP cord-304685-s0bfhwtn 128 4 ~ ~ NNP cord-304685-s0bfhwtn 128 5 ion ion NN cord-304685-s0bfhwtn 128 6 , , , cord-304685-s0bfhwtn 128 7 quiescent quiescent JJ cord-304685-s0bfhwtn 128 8 cells cell NNS cord-304685-s0bfhwtn 128 9 were be VBD cord-304685-s0bfhwtn 128 10 infected infect VBN cord-304685-s0bfhwtn 128 11 with with IN cord-304685-s0bfhwtn 128 12 5 5 CD cord-304685-s0bfhwtn 128 13 ml ml NNS cord-304685-s0bfhwtn 128 14 of of IN cord-304685-s0bfhwtn 128 15 HSV-1 hsv-1 NN cord-304685-s0bfhwtn 128 16 with with IN cord-304685-s0bfhwtn 128 17 a a DT cord-304685-s0bfhwtn 128 18 titer titer NN cord-304685-s0bfhwtn 128 19 of of IN cord-304685-s0bfhwtn 128 20 107 107 CD cord-304685-s0bfhwtn 128 21 PFU pfu NN cord-304685-s0bfhwtn 128 22 / / SYM cord-304685-s0bfhwtn 128 23 ml ml NN cord-304685-s0bfhwtn 128 24 for for IN cord-304685-s0bfhwtn 128 25 2 2 CD cord-304685-s0bfhwtn 128 26 h h NN cord-304685-s0bfhwtn 128 27 at at IN cord-304685-s0bfhwtn 128 28 37~ 37~ CD cord-304685-s0bfhwtn 128 29 with with IN cord-304685-s0bfhwtn 128 30 rocking rock VBG cord-304685-s0bfhwtn 128 31 every every DT cord-304685-s0bfhwtn 128 32 15 15 CD cord-304685-s0bfhwtn 128 33 rain rain NN cord-304685-s0bfhwtn 128 34 . . . cord-304685-s0bfhwtn 129 1 Multiplicity multiplicity NN cord-304685-s0bfhwtn 129 2 of of IN cord-304685-s0bfhwtn 129 3 infection infection NN cord-304685-s0bfhwtn 129 4 , , , cord-304685-s0bfhwtn 129 5 determined determine VBN cord-304685-s0bfhwtn 129 6 after after IN cord-304685-s0bfhwtn 129 7 infection infection NN cord-304685-s0bfhwtn 129 8 by by IN cord-304685-s0bfhwtn 129 9 counting count VBG cord-304685-s0bfhwtn 129 10 the the DT cord-304685-s0bfhwtn 129 11 number number NN cord-304685-s0bfhwtn 129 12 of of IN cord-304685-s0bfhwtn 129 13 cells cell NNS cord-304685-s0bfhwtn 129 14 in in IN cord-304685-s0bfhwtn 129 15 the the DT cord-304685-s0bfhwtn 129 16 infected infected JJ cord-304685-s0bfhwtn 129 17 flask flask NN cord-304685-s0bfhwtn 129 18 , , , cord-304685-s0bfhwtn 129 19 was be VBD cord-304685-s0bfhwtn 129 20 20 20 CD cord-304685-s0bfhwtn 129 21 . . . cord-304685-s0bfhwtn 130 1 Target target NN cord-304685-s0bfhwtn 130 2 cells cell NNS cord-304685-s0bfhwtn 130 3 were be VBD cord-304685-s0bfhwtn 130 4 processed process VBN cord-304685-s0bfhwtn 130 5 and and CC cord-304685-s0bfhwtn 130 6 assayed assay VBN cord-304685-s0bfhwtn 130 7 as as IN cord-304685-s0bfhwtn 130 8 in in IN cord-304685-s0bfhwtn 130 9 Fig Fig NNP cord-304685-s0bfhwtn 130 10 . . . cord-304685-s0bfhwtn 131 1 1 1 CD cord-304685-s0bfhwtn 131 2 in in IN cord-304685-s0bfhwtn 131 3 AIM aim NN cord-304685-s0bfhwtn 131 4 - - HYPH cord-304685-s0bfhwtn 131 5 V v NN cord-304685-s0bfhwtn 131 6 media medium NNS cord-304685-s0bfhwtn 131 7 without without IN cord-304685-s0bfhwtn 131 8 or or CC cord-304685-s0bfhwtn 131 9 with with IN cord-304685-s0bfhwtn 131 10 10 10 CD cord-304685-s0bfhwtn 131 11 % % NN cord-304685-s0bfhwtn 131 12 FBS FBS NNP cord-304685-s0bfhwtn 131 13 as as IN cord-304685-s0bfhwtn 131 14 indicated indicate VBN cord-304685-s0bfhwtn 131 15 . . . cord-304685-s0bfhwtn 132 1 Cytotoxicity cytotoxicity NN cord-304685-s0bfhwtn 132 2 assays assay NNS cord-304685-s0bfhwtn 132 3 were be VBD cord-304685-s0bfhwtn 132 4 performed perform VBN cord-304685-s0bfhwtn 132 5 as as IN cord-304685-s0bfhwtn 132 6 described describe VBN cord-304685-s0bfhwtn 132 7 ( ( -LRB- cord-304685-s0bfhwtn 132 8 3 3 CD cord-304685-s0bfhwtn 132 9 ) ) -RRB- cord-304685-s0bfhwtn 132 10 . . . cord-304685-s0bfhwtn 133 1 Assay assay NN cord-304685-s0bfhwtn 133 2 time time NN cord-304685-s0bfhwtn 133 3 for for IN cord-304685-s0bfhwtn 133 4 the the DT cord-304685-s0bfhwtn 133 5 data datum NNS cord-304685-s0bfhwtn 133 6 presented present VBN cord-304685-s0bfhwtn 133 7 was be VBD cord-304685-s0bfhwtn 133 8 14 14 CD cord-304685-s0bfhwtn 133 9 h. h. NN cord-304685-s0bfhwtn 133 10 Time time NN cord-304685-s0bfhwtn 133 11 = = SYM cord-304685-s0bfhwtn 133 12 0 0 NFP cord-304685-s0bfhwtn 133 13 when when WRB cord-304685-s0bfhwtn 133 14 the the DT cord-304685-s0bfhwtn 133 15 LCMV LCMV NNP cord-304685-s0bfhwtn 133 16 - - HYPH cord-304685-s0bfhwtn 133 17 CTLs CTLs NNP cord-304685-s0bfhwtn 133 18 were be VBD cord-304685-s0bfhwtn 133 19 added add VBN cord-304685-s0bfhwtn 133 20 to to IN cord-304685-s0bfhwtn 133 21 the the DT cord-304685-s0bfhwtn 133 22 target target NN cord-304685-s0bfhwtn 133 23 cells cell NNS cord-304685-s0bfhwtn 133 24 . . . cord-304685-s0bfhwtn 134 1 Con Con NNP cord-304685-s0bfhwtn 134 2 A A NNP cord-304685-s0bfhwtn 134 3 was be VBD cord-304685-s0bfhwtn 134 4 at at IN cord-304685-s0bfhwtn 134 5 a a DT cord-304685-s0bfhwtn 134 6 final final JJ cord-304685-s0bfhwtn 134 7 concentration concentration NN cord-304685-s0bfhwtn 134 8 of of IN cord-304685-s0bfhwtn 134 9 5/~g 5/~g CD cord-304685-s0bfhwtn 134 10 / / SYM cord-304685-s0bfhwtn 134 11 rrd rrd NN cord-304685-s0bfhwtn 134 12 . . . cord-304685-s0bfhwtn 135 1 Total total JJ cord-304685-s0bfhwtn 135 2 time time NN cord-304685-s0bfhwtn 135 3 from from IN cord-304685-s0bfhwtn 135 4 the the DT cord-304685-s0bfhwtn 135 5 HSV-1 HSV-1 NNP cord-304685-s0bfhwtn 135 6 infection infection NN cord-304685-s0bfhwtn 135 7 of of IN cord-304685-s0bfhwtn 135 8 quiescent quiescent JJ cord-304685-s0bfhwtn 135 9 targets target NNS cord-304685-s0bfhwtn 135 10 to to IN cord-304685-s0bfhwtn 135 11 harvest harvest VB cord-304685-s0bfhwtn 135 12 of of IN cord-304685-s0bfhwtn 135 13 the the DT cord-304685-s0bfhwtn 135 14 assay assay NN cord-304685-s0bfhwtn 135 15 was be VBD cord-304685-s0bfhwtn 135 16 20 20 CD cord-304685-s0bfhwtn 135 17 h. h. NN cord-304685-s0bfhwtn 135 18 tamination tamination NN cord-304685-s0bfhwtn 135 19 of of IN cord-304685-s0bfhwtn 135 20 the the DT cord-304685-s0bfhwtn 135 21 virus virus NN cord-304685-s0bfhwtn 135 22 inoculum inoculum NN cord-304685-s0bfhwtn 135 23 , , , cord-304685-s0bfhwtn 135 24 because because IN cord-304685-s0bfhwtn 135 25 FBS FBS NNP cord-304685-s0bfhwtn 135 26 added add VBD cord-304685-s0bfhwtn 135 27 back back RB cord-304685-s0bfhwtn 135 28 to to IN cord-304685-s0bfhwtn 135 29 quiescent quiescent JJ cord-304685-s0bfhwtn 135 30 targets target NNS cord-304685-s0bfhwtn 135 31 or or CC cord-304685-s0bfhwtn 135 32 to to IN cord-304685-s0bfhwtn 135 33 HSV HSV NNP cord-304685-s0bfhwtn 135 34 - - HYPH cord-304685-s0bfhwtn 135 35 l l NN cord-304685-s0bfhwtn 135 36 - - HYPH cord-304685-s0bfhwtn 135 37 infected infected JJ cord-304685-s0bfhwtn 135 38 quiescent quiescent JJ cord-304685-s0bfhwtn 135 39 targets target NNS cord-304685-s0bfhwtn 135 40 , , , cord-304685-s0bfhwtn 135 41 to to IN cord-304685-s0bfhwtn 135 42 a a DT cord-304685-s0bfhwtn 135 43 final final JJ cord-304685-s0bfhwtn 135 44 concentration concentration NN cord-304685-s0bfhwtn 135 45 of of IN cord-304685-s0bfhwtn 135 46 10 10 CD cord-304685-s0bfhwtn 135 47 % % NN cord-304685-s0bfhwtn 135 48 , , , cord-304685-s0bfhwtn 135 49 did do VBD cord-304685-s0bfhwtn 135 50 not not RB cord-304685-s0bfhwtn 135 51 significantly significantly RB cord-304685-s0bfhwtn 135 52 increase increase VB cord-304685-s0bfhwtn 135 53 their -PRON- PRP$ cord-304685-s0bfhwtn 135 54 susceptibility susceptibility NN cord-304685-s0bfhwtn 135 55 to to IN cord-304685-s0bfhwtn 135 56 CTL CTL NNP cord-304685-s0bfhwtn 135 57 - - HYPH cord-304685-s0bfhwtn 135 58 induced induce VBN cord-304685-s0bfhwtn 135 59 apoptosis apoptosis NN cord-304685-s0bfhwtn 136 1 ( ( -LRB- cord-304685-s0bfhwtn 136 2 Fig Fig NNP cord-304685-s0bfhwtn 136 3 . . NNP cord-304685-s0bfhwtn 136 4 2 2 CD cord-304685-s0bfhwtn 136 5 , , , cord-304685-s0bfhwtn 136 6 A a NN cord-304685-s0bfhwtn 136 7 and and CC cord-304685-s0bfhwtn 136 8 _SP cord-304685-s0bfhwtn 136 9 B B NNP cord-304685-s0bfhwtn 136 10 ) ) -RRB- cord-304685-s0bfhwtn 136 11 . . . cord-304685-s0bfhwtn 137 1 The the DT cord-304685-s0bfhwtn 137 2 possibility possibility NN cord-304685-s0bfhwtn 137 3 that that IN cord-304685-s0bfhwtn 137 4 HSV-1 HSV-1 NNP cord-304685-s0bfhwtn 137 5 directly directly RB cord-304685-s0bfhwtn 137 6 induced induce VBN cord-304685-s0bfhwtn 137 7 fragmentation fragmentation NN cord-304685-s0bfhwtn 137 8 of of IN cord-304685-s0bfhwtn 137 9 the the DT cord-304685-s0bfhwtn 137 10 target target NN cord-304685-s0bfhwtn 137 11 cell cell NN cord-304685-s0bfhwtn 137 12 DNA dna NN cord-304685-s0bfhwtn 137 13 is be VBZ cord-304685-s0bfhwtn 137 14 unlikely unlikely JJ cord-304685-s0bfhwtn 137 15 , , , cord-304685-s0bfhwtn 137 16 as as IN cord-304685-s0bfhwtn 137 17 the the DT cord-304685-s0bfhwtn 137 18 control control NN cord-304685-s0bfhwtn 137 19 virusinfected virusinfecte VBD cord-304685-s0bfhwtn 137 20 samples sample NNS cord-304685-s0bfhwtn 137 21 did do VBD cord-304685-s0bfhwtn 137 22 not not RB cord-304685-s0bfhwtn 137 23 show show VB cord-304685-s0bfhwtn 137 24 oligonudeosomal oligonudeosomal NN cord-304685-s0bfhwtn 137 25 ladder ladder NN cord-304685-s0bfhwtn 137 26 proteins protein NNS cord-304685-s0bfhwtn 137 27 , , , cord-304685-s0bfhwtn 137 28 nor nor CC cord-304685-s0bfhwtn 137 29 was be VBD cord-304685-s0bfhwtn 137 30 the the DT cord-304685-s0bfhwtn 137 31 level level NN cord-304685-s0bfhwtn 137 32 of of IN cord-304685-s0bfhwtn 137 33 spontaneous spontaneous JJ cord-304685-s0bfhwtn 137 34 DNA dna NN cord-304685-s0bfhwtn 137 35 release release NN cord-304685-s0bfhwtn 137 36 different different JJ cord-304685-s0bfhwtn 137 37 from from IN cord-304685-s0bfhwtn 137 38 uninfected uninfected JJ cord-304685-s0bfhwtn 137 39 target target NN cord-304685-s0bfhwtn 137 40 cells cell NNS cord-304685-s0bfhwtn 137 41 . . . cord-304685-s0bfhwtn 138 1 Evaluation evaluation NN cord-304685-s0bfhwtn 138 2 of of IN cord-304685-s0bfhwtn 138 3 whether whether IN cord-304685-s0bfhwtn 138 4 HSV-1 HSV-1 NNP cord-304685-s0bfhwtn 138 5 infection infection NN cord-304685-s0bfhwtn 138 6 could could MD cord-304685-s0bfhwtn 138 7 directly directly RB cord-304685-s0bfhwtn 138 8 induce induce VB cord-304685-s0bfhwtn 138 9 apoptosis apoptosis NN cord-304685-s0bfhwtn 138 10 revealed reveal VBD cord-304685-s0bfhwtn 138 11 that that IN cord-304685-s0bfhwtn 138 12 the the DT cord-304685-s0bfhwtn 138 13 HSV-1 HSV-1 NNP cord-304685-s0bfhwtn 138 14 cytopathic cytopathic JJ cord-304685-s0bfhwtn 138 15 effect effect NN cord-304685-s0bfhwtn 138 16 kills kill VBZ cord-304685-s0bfhwtn 138 17 cells cell NNS cord-304685-s0bfhwtn 138 18 via via IN cord-304685-s0bfhwtn 138 19 necrosis necrosis NN cord-304685-s0bfhwtn 138 20 ( ( -LRB- cord-304685-s0bfhwtn 138 21 data datum NNS cord-304685-s0bfhwtn 138 22 not not RB cord-304685-s0bfhwtn 138 23 shown show VBN cord-304685-s0bfhwtn 138 24 ) ) -RRB- cord-304685-s0bfhwtn 138 25 , , , cord-304685-s0bfhwtn 138 26 consistent consistent JJ cord-304685-s0bfhwtn 138 27 with with IN cord-304685-s0bfhwtn 138 28 a a DT cord-304685-s0bfhwtn 138 29 recent recent JJ cord-304685-s0bfhwtn 138 30 report report NN cord-304685-s0bfhwtn 138 31 ( ( -LRB- cord-304685-s0bfhwtn 138 32 24 24 CD cord-304685-s0bfhwtn 138 33 ) ) -RRB- cord-304685-s0bfhwtn 138 34 . . . cord-304685-s0bfhwtn 139 1 In in IN cord-304685-s0bfhwtn 139 2 preliminary preliminary JJ cord-304685-s0bfhwtn 139 3 studies study NNS cord-304685-s0bfhwtn 139 4 , , , cord-304685-s0bfhwtn 139 5 various various JJ cord-304685-s0bfhwtn 139 6 RNA RNA NNP cord-304685-s0bfhwtn 139 7 viruses virus NNS cord-304685-s0bfhwtn 139 8 , , , cord-304685-s0bfhwtn 139 9 i.e. i.e. FW cord-304685-s0bfhwtn 139 10 , , , cord-304685-s0bfhwtn 139 11 the the DT cord-304685-s0bfhwtn 139 12 coronavirus coronavirus NN cord-304685-s0bfhwtn 139 13 mouse mouse NN cord-304685-s0bfhwtn 139 14 hepatitis hepatitis NN cord-304685-s0bfhwtn 139 15 virus virus NN cord-304685-s0bfhwtn 139 16 , , , cord-304685-s0bfhwtn 139 17 strain strain VB cord-304685-s0bfhwtn 139 18 A-59 A-59 NNP cord-304685-s0bfhwtn 139 19 , , , cord-304685-s0bfhwtn 139 20 the the DT cord-304685-s0bfhwtn 139 21 picornavirus picornavirus NNP cord-304685-s0bfhwtn 139 22 encephalomyocarditis encephalomyocarditis NNP cord-304685-s0bfhwtn 139 23 virus virus NN cord-304685-s0bfhwtn 139 24 , , , cord-304685-s0bfhwtn 139 25 and and CC cord-304685-s0bfhwtn 139 26 LCMV LCMV NNP cord-304685-s0bfhwtn 139 27 , , , cord-304685-s0bfhwtn 139 28 were be VBD cord-304685-s0bfhwtn 139 29 unable unable JJ cord-304685-s0bfhwtn 139 30 to to TO cord-304685-s0bfhwtn 139 31 render render VB cord-304685-s0bfhwtn 139 32 the the DT cord-304685-s0bfhwtn 139 33 quiescent quiescent JJ cord-304685-s0bfhwtn 139 34 targets target NNS cord-304685-s0bfhwtn 139 35 more more RBR cord-304685-s0bfhwtn 139 36 susceptible susceptible JJ cord-304685-s0bfhwtn 139 37 to to IN cord-304685-s0bfhwtn 139 38 CTL CTL NNP cord-304685-s0bfhwtn 139 39 - - HYPH cord-304685-s0bfhwtn 139 40 induced induce VBN cord-304685-s0bfhwtn 139 41 apoptosis apoptosis NN cord-304685-s0bfhwtn 139 42 , , , cord-304685-s0bfhwtn 139 43 nor nor CC cord-304685-s0bfhwtn 139 44 did do VBD cord-304685-s0bfhwtn 139 45 they -PRON- PRP cord-304685-s0bfhwtn 139 46 stimulate stimulate VB cord-304685-s0bfhwtn 139 47 DNA dna NN cord-304685-s0bfhwtn 139 48 synthesis synthesis NN cord-304685-s0bfhwtn 139 49 in in IN cord-304685-s0bfhwtn 139 50 these these DT cord-304685-s0bfhwtn 139 51 cells cell NNS cord-304685-s0bfhwtn 139 52 . . . cord-304685-s0bfhwtn 140 1 However however RB cord-304685-s0bfhwtn 140 2 , , , cord-304685-s0bfhwtn 140 3 vaccinia vaccinia NN cord-304685-s0bfhwtn 140 4 virus virus NN cord-304685-s0bfhwtn 140 5 , , , cord-304685-s0bfhwtn 140 6 a a DT cord-304685-s0bfhwtn 140 7 DNA dna NN cord-304685-s0bfhwtn 140 8 virus virus NN cord-304685-s0bfhwtn 140 9 that that WDT cord-304685-s0bfhwtn 140 10 encodes encode VBZ cord-304685-s0bfhwtn 140 11 its -PRON- PRP$ cord-304685-s0bfhwtn 140 12 own own JJ cord-304685-s0bfhwtn 140 13 DNA dna NN cord-304685-s0bfhwtn 141 1 topoisomerase topoisomerase NNP cord-304685-s0bfhwtn 142 1 I -PRON- PRP cord-304685-s0bfhwtn 142 2 and and CC cord-304685-s0bfhwtn 142 3 other other JJ cord-304685-s0bfhwtn 142 4 proteins protein NNS cord-304685-s0bfhwtn 142 5 that that WDT cord-304685-s0bfhwtn 142 6 interact interact VBP cord-304685-s0bfhwtn 142 7 with with IN cord-304685-s0bfhwtn 142 8 cell cell NN cord-304685-s0bfhwtn 142 9 cycle cycle NN cord-304685-s0bfhwtn 142 10 progression progression NN cord-304685-s0bfhwtn 142 11 factors factor NNS cord-304685-s0bfhwtn 142 12 ( ( -LRB- cord-304685-s0bfhwtn 142 13 25 25 CD cord-304685-s0bfhwtn 142 14 ) ) -RRB- cord-304685-s0bfhwtn 142 15 , , , cord-304685-s0bfhwtn 142 16 increased increase VBD cord-304685-s0bfhwtn 142 17 the the DT cord-304685-s0bfhwtn 142 18 susceptibility susceptibility NN cord-304685-s0bfhwtn 142 19 of of IN cord-304685-s0bfhwtn 142 20 quiescent quiescent JJ cord-304685-s0bfhwtn 142 21 targets target NNS cord-304685-s0bfhwtn 142 22 to to IN cord-304685-s0bfhwtn 142 23 CTL CTL NNP cord-304685-s0bfhwtn 142 24 - - HYPH cord-304685-s0bfhwtn 142 25 induced induce VBN cord-304685-s0bfhwtn 142 26 apoptosis apoptosis NN cord-304685-s0bfhwtn 142 27 . . . cord-304685-s0bfhwtn 143 1 The the DT cord-304685-s0bfhwtn 143 2 data datum NNS cord-304685-s0bfhwtn 143 3 presented present VBD cord-304685-s0bfhwtn 143 4 suggest suggest VBP cord-304685-s0bfhwtn 143 5 that that IN cord-304685-s0bfhwtn 143 6 cells cell NNS cord-304685-s0bfhwtn 143 7 must must MD cord-304685-s0bfhwtn 143 8 be be VB cord-304685-s0bfhwtn 143 9 in in IN cord-304685-s0bfhwtn 143 10 cycle cycle NN cord-304685-s0bfhwtn 143 11 or or CC cord-304685-s0bfhwtn 143 12 in in IN cord-304685-s0bfhwtn 143 13 a a DT cord-304685-s0bfhwtn 143 14 state state NN cord-304685-s0bfhwtn 143 15 of of IN cord-304685-s0bfhwtn 143 16 competence competence NN cord-304685-s0bfhwtn 143 17 to to TO cord-304685-s0bfhwtn 143 18 be be VB cord-304685-s0bfhwtn 143 19 susceptible susceptible JJ cord-304685-s0bfhwtn 143 20 to to IN cord-304685-s0bfhwtn 143 21 CTL CTL NNP cord-304685-s0bfhwtn 143 22 - - HYPH cord-304685-s0bfhwtn 143 23 induced induce VBN cord-304685-s0bfhwtn 143 24 apoptosis apoptosis NN cord-304685-s0bfhwtn 143 25 . . . cord-304685-s0bfhwtn 144 1 Transformation transformation NN cord-304685-s0bfhwtn 144 2 of of IN cord-304685-s0bfhwtn 144 3 cells cell NNS cord-304685-s0bfhwtn 144 4 with with IN cord-304685-s0bfhwtn 144 5 c c NNP cord-304685-s0bfhwtn 144 6 - - HYPH cord-304685-s0bfhwtn 144 7 myc myc NNP cord-304685-s0bfhwtn 144 8 enhanced enhance VBD cord-304685-s0bfhwtn 144 9 their -PRON- PRP$ cord-304685-s0bfhwtn 144 10 growth growth NN cord-304685-s0bfhwtn 144 11 rate rate NN cord-304685-s0bfhwtn 144 12 and and CC cord-304685-s0bfhwtn 144 13 rendered render VBD cord-304685-s0bfhwtn 144 14 them -PRON- PRP cord-304685-s0bfhwtn 144 15 very very RB cord-304685-s0bfhwtn 144 16 susceptible susceptible JJ cord-304685-s0bfhwtn 144 17 to to IN cord-304685-s0bfhwtn 144 18 CTLinduced ctlinduce VBN cord-304685-s0bfhwtn 144 19 apoptosis apoptosis NN cord-304685-s0bfhwtn 144 20 , , , cord-304685-s0bfhwtn 144 21 but but CC cord-304685-s0bfhwtn 144 22 the the DT cord-304685-s0bfhwtn 144 23 specific specific JJ cord-304685-s0bfhwtn 144 24 role role NN cord-304685-s0bfhwtn 144 25 of of IN cord-304685-s0bfhwtn 144 26 c c NNP cord-304685-s0bfhwtn 144 27 - - HYPH cord-304685-s0bfhwtn 144 28 myc myc NNP cord-304685-s0bfhwtn 144 29 in in IN cord-304685-s0bfhwtn 144 30 apoptosis apoptosis NN cord-304685-s0bfhwtn 144 31 is be VBZ cord-304685-s0bfhwtn 144 32 unclear unclear JJ cord-304685-s0bfhwtn 144 33 . . . cord-304685-s0bfhwtn 145 1 Our -PRON- PRP$ cord-304685-s0bfhwtn 145 2 analyses analysis NNS cord-304685-s0bfhwtn 145 3 by by IN cord-304685-s0bfhwtn 145 4 immunofluorescence immunofluorescence NN cord-304685-s0bfhwtn 145 5 of of IN cord-304685-s0bfhwtn 145 6 c c NN cord-304685-s0bfhwtn 145 7 - - HYPH cord-304685-s0bfhwtn 145 8 rayc rayc NN cord-304685-s0bfhwtn 145 9 protein protein NN cord-304685-s0bfhwtn 145 10 levels level NNS cord-304685-s0bfhwtn 145 11 between between IN cord-304685-s0bfhwtn 145 12 the the DT cord-304685-s0bfhwtn 145 13 targets target NNS cord-304685-s0bfhwtn 145 14 shown show VBN cord-304685-s0bfhwtn 145 15 in in IN cord-304685-s0bfhwtn 145 16 Figs Figs NNPS cord-304685-s0bfhwtn 145 17 . . . cord-304685-s0bfhwtn 146 1 3 3 CD cord-304685-s0bfhwtn 146 2 and and CC cord-304685-s0bfhwtn 146 3 4 4 CD cord-304685-s0bfhwtn 146 4 did do VBD cord-304685-s0bfhwtn 146 5 not not RB cord-304685-s0bfhwtn 146 6 reveal reveal VB cord-304685-s0bfhwtn 146 7 a a DT cord-304685-s0bfhwtn 146 8 corresponding corresponding JJ cord-304685-s0bfhwtn 146 9 relationship relationship NN cord-304685-s0bfhwtn 146 10 with with IN cord-304685-s0bfhwtn 146 11 their -PRON- PRP$ cord-304685-s0bfhwtn 146 12 levels level NNS cord-304685-s0bfhwtn 146 13 of of IN cord-304685-s0bfhwtn 146 14 DNA dna NN cord-304685-s0bfhwtn 146 15 fragmentation fragmentation NN cord-304685-s0bfhwtn 146 16 . . . cord-304685-s0bfhwtn 147 1 Since since IN cord-304685-s0bfhwtn 147 2 others other NNS cord-304685-s0bfhwtn 147 3 have have VBP cord-304685-s0bfhwtn 147 4 shown show VBN cord-304685-s0bfhwtn 147 5 that that DT cord-304685-s0bfhwtn 147 6 antisense antisense NN cord-304685-s0bfhwtn 147 7 to to IN cord-304685-s0bfhwtn 147 8 c c NNP cord-304685-s0bfhwtn 147 9 - - HYPH cord-304685-s0bfhwtn 147 10 myc myc NNP cord-304685-s0bfhwtn 147 11 can can MD cord-304685-s0bfhwtn 147 12 block block VB cord-304685-s0bfhwtn 147 13 antigen antigen NN cord-304685-s0bfhwtn 147 14 receptor receptor NN cord-304685-s0bfhwtn 147 15 - - HYPH cord-304685-s0bfhwtn 147 16 induced induce VBN cord-304685-s0bfhwtn 147 17 apoptosis apoptosis NN cord-304685-s0bfhwtn 147 18 in in IN cord-304685-s0bfhwtn 147 19 T t NN cord-304685-s0bfhwtn 147 20 cells cell NNS cord-304685-s0bfhwtn 147 21 ( ( -LRB- cord-304685-s0bfhwtn 147 22 26 26 CD cord-304685-s0bfhwtn 147 23 ) ) -RRB- cord-304685-s0bfhwtn 147 24 , , , cord-304685-s0bfhwtn 147 25 we -PRON- PRP cord-304685-s0bfhwtn 147 26 asked ask VBD cord-304685-s0bfhwtn 147 27 whether whether IN cord-304685-s0bfhwtn 147 28 similar similar JJ cord-304685-s0bfhwtn 147 29 treatment treatment NN cord-304685-s0bfhwtn 147 30 of of IN cord-304685-s0bfhwtn 147 31 target target NN cord-304685-s0bfhwtn 147 32 cells cell NNS cord-304685-s0bfhwtn 147 33 would would MD cord-304685-s0bfhwtn 147 34 block block VB cord-304685-s0bfhwtn 147 35 CTL CTL NNP cord-304685-s0bfhwtn 147 36 - - HYPH cord-304685-s0bfhwtn 147 37 induced induce VBN cord-304685-s0bfhwtn 147 38 apoptosis apoptosis NN cord-304685-s0bfhwtn 147 39 . . . cord-304685-s0bfhwtn 148 1 Although although IN cord-304685-s0bfhwtn 148 2 a a DT cord-304685-s0bfhwtn 148 3 4 4 CD cord-304685-s0bfhwtn 148 4 - - SYM cord-304685-s0bfhwtn 148 5 18-h 18-h CD cord-304685-s0bfhwtn 148 6 treatment treatment NN cord-304685-s0bfhwtn 148 7 of of IN cord-304685-s0bfhwtn 148 8 P815 p815 PRP cord-304685-s0bfhwtn 148 9 and and CC cord-304685-s0bfhwtn 148 10 L929 l929 CD cord-304685-s0bfhwtn 148 11 cells cell NNS cord-304685-s0bfhwtn 148 12 with with IN cord-304685-s0bfhwtn 148 13 antisense antisense NN cord-304685-s0bfhwtn 148 14 to to IN cord-304685-s0bfhwtn 148 15 c c NNP cord-304685-s0bfhwtn 148 16 - - HYPH cord-304685-s0bfhwtn 148 17 myc myc NNP cord-304685-s0bfhwtn 148 18 ( ( -LRB- cord-304685-s0bfhwtn 148 19 5'-CACGTGAGGGGCAT-3 5'-cacgtgaggggcat-3 CD cord-304685-s0bfhwtn 148 20 ' ' '' cord-304685-s0bfhwtn 148 21 ) ) -RRB- cord-304685-s0bfhwtn 148 22 significantly significantly RB cord-304685-s0bfhwtn 148 23 reduced reduce VBN cord-304685-s0bfhwtn 148 24 myc myc NNP cord-304685-s0bfhwtn 148 25 protein protein NN cord-304685-s0bfhwtn 148 26 levels level NNS cord-304685-s0bfhwtn 148 27 and and CC cord-304685-s0bfhwtn 148 28 arrested arrest VBD cord-304685-s0bfhwtn 148 29 the the DT cord-304685-s0bfhwtn 148 30 cells cell NNS cord-304685-s0bfhwtn 148 31 in in IN cord-304685-s0bfhwtn 148 32 the the DT cord-304685-s0bfhwtn 148 33 Gt Gt NNP cord-304685-s0bfhwtn 148 34 ( ( -LRB- cord-304685-s0bfhwtn 148 35 but but CC cord-304685-s0bfhwtn 148 36 not not RB cord-304685-s0bfhwtn 148 37 Go go VB cord-304685-s0bfhwtn 148 38 ) ) -RRB- cord-304685-s0bfhwtn 148 39 phase phase NN cord-304685-s0bfhwtn 148 40 of of IN cord-304685-s0bfhwtn 148 41 the the DT cord-304685-s0bfhwtn 148 42 cell cell NN cord-304685-s0bfhwtn 148 43 cycle cycle NN cord-304685-s0bfhwtn 148 44 , , , cord-304685-s0bfhwtn 148 45 as as IN cord-304685-s0bfhwtn 148 46 shown show VBN cord-304685-s0bfhwtn 148 47 previously previously RB cord-304685-s0bfhwtn 148 48 ( ( -LRB- cord-304685-s0bfhwtn 148 49 27 27 CD cord-304685-s0bfhwtn 148 50 ) ) -RRB- cord-304685-s0bfhwtn 148 51 , , , cord-304685-s0bfhwtn 148 52 it -PRON- PRP cord-304685-s0bfhwtn 148 53 did do VBD cord-304685-s0bfhwtn 148 54 not not RB cord-304685-s0bfhwtn 148 55 impair impair VB cord-304685-s0bfhwtn 148 56 the the DT cord-304685-s0bfhwtn 148 57 level level NN cord-304685-s0bfhwtn 148 58 of of IN cord-304685-s0bfhwtn 148 59 CTL CTL NNP cord-304685-s0bfhwtn 148 60 - - HYPH cord-304685-s0bfhwtn 148 61 induced induce VBN cord-304685-s0bfhwtn 148 62 DNA dna NN cord-304685-s0bfhwtn 148 63 fragmentation fragmentation NN cord-304685-s0bfhwtn 148 64 versus versus IN cord-304685-s0bfhwtn 148 65 untreated untreated JJ cord-304685-s0bfhwtn 148 66 targets target NNS cord-304685-s0bfhwtn 148 67 ( ( -LRB- cord-304685-s0bfhwtn 148 68 data datum NNS cord-304685-s0bfhwtn 148 69 not not RB cord-304685-s0bfhwtn 148 70 shown show VBN cord-304685-s0bfhwtn 148 71 ) ) -RRB- cord-304685-s0bfhwtn 148 72 . . . cord-304685-s0bfhwtn 149 1 This this DT cord-304685-s0bfhwtn 149 2 suggests suggest VBZ cord-304685-s0bfhwtn 149 3 that that IN cord-304685-s0bfhwtn 149 4 c c NNP cord-304685-s0bfhwtn 149 5 - - HYPH cord-304685-s0bfhwtn 149 6 myr myr NNP cord-304685-s0bfhwtn 149 7 , , , cord-304685-s0bfhwtn 149 8 per per FW cord-304685-s0bfhwtn 149 9 se se FW cord-304685-s0bfhwtn 149 10 , , , cord-304685-s0bfhwtn 149 11 is be VBZ cord-304685-s0bfhwtn 149 12 only only RB cord-304685-s0bfhwtn 149 13 indirectly indirectly RB cord-304685-s0bfhwtn 149 14 involved involve VBN cord-304685-s0bfhwtn 149 15 in in IN cord-304685-s0bfhwtn 149 16 rendering render VBG cord-304685-s0bfhwtn 149 17 targets target NNS cord-304685-s0bfhwtn 149 18 sensitive sensitive JJ cord-304685-s0bfhwtn 149 19 to to IN cord-304685-s0bfhwtn 149 20 CTL CTL NNP cord-304685-s0bfhwtn 149 21 - - HYPH cord-304685-s0bfhwtn 149 22 induced induce VBN cord-304685-s0bfhwtn 149 23 apoptosis apoptosis NN cord-304685-s0bfhwtn 149 24 . . . cord-304685-s0bfhwtn 150 1 HSV-1 HSV-1 NNS cord-304685-s0bfhwtn 150 2 can can MD cord-304685-s0bfhwtn 150 3 induce induce VB cord-304685-s0bfhwtn 150 4 the the DT cord-304685-s0bfhwtn 150 5 up up JJ cord-304685-s0bfhwtn 150 6 - - HYPH cord-304685-s0bfhwtn 150 7 regulation regulation NN cord-304685-s0bfhwtn 150 8 of of IN cord-304685-s0bfhwtn 150 9 the the DT cord-304685-s0bfhwtn 150 10 immediate immediate JJ cord-304685-s0bfhwtn 150 11 early early JJ cord-304685-s0bfhwtn 150 12 ( ( -LRB- cord-304685-s0bfhwtn 150 13 IE IE NNP cord-304685-s0bfhwtn 150 14 ) ) -RRB- cord-304685-s0bfhwtn 150 15 transcription transcription NN cord-304685-s0bfhwtn 150 16 factor factor NN cord-304685-s0bfhwtn 150 17 c c NNP cord-304685-s0bfhwtn 150 18 - - : cord-304685-s0bfhwtn 150 19 jun jun NNP cord-304685-s0bfhwtn 150 20 / / SYM cord-304685-s0bfhwtn 150 21 AP-1 AP-1 NNP cord-304685-s0bfhwtn 150 22 ( ( -LRB- cord-304685-s0bfhwtn 150 23 c c NNP cord-304685-s0bfhwtn 150 24 - - HYPH cord-304685-s0bfhwtn 150 25 jun jun NNP cord-304685-s0bfhwtn 150 26 ) ) -RRB- cord-304685-s0bfhwtn 150 27 ( ( -LRB- cord-304685-s0bfhwtn 150 28 28 28 CD cord-304685-s0bfhwtn 150 29 ) ) -RRB- cord-304685-s0bfhwtn 150 30 . . . cord-304685-s0bfhwtn 151 1 c c NNP cord-304685-s0bfhwtn 151 2 - - : cord-304685-s0bfhwtn 151 3 jun jun NNP cord-304685-s0bfhwtn 151 4 and and CC cord-304685-s0bfhwtn 151 5 c c NNP cord-304685-s0bfhwtn 151 6 - - HYPH cord-304685-s0bfhwtn 151 7 fos fos NNP cord-304685-s0bfhwtn 151 8 are be VBP cord-304685-s0bfhwtn 151 9 required require VBN cord-304685-s0bfhwtn 151 10 for for IN cord-304685-s0bfhwtn 151 11 the the DT cord-304685-s0bfhwtn 151 12 serum serum NN cord-304685-s0bfhwtn 151 13 - - HYPH cord-304685-s0bfhwtn 151 14 stimulated stimulate VBN cord-304685-s0bfhwtn 151 15 transition transition NN cord-304685-s0bfhwtn 151 16 out out IN cord-304685-s0bfhwtn 151 17 of of IN cord-304685-s0bfhwtn 151 18 Go go VB cord-304685-s0bfhwtn 151 19 into into IN cord-304685-s0bfhwtn 151 20 G1 G1 NNP cord-304685-s0bfhwtn 151 21 ( ( -LRB- cord-304685-s0bfhwtn 151 22 29 29 CD cord-304685-s0bfhwtn 151 23 ) ) -RRB- cord-304685-s0bfhwtn 151 24 . . . cord-304685-s0bfhwtn 152 1 The the DT cord-304685-s0bfhwtn 152 2 c c NNP cord-304685-s0bfhwtn 152 3 - - HYPH cord-304685-s0bfhwtn 152 4 jun jun NNP cord-304685-s0bfhwtn 152 5 - - HYPH cord-304685-s0bfhwtn 152 6 inducing induce VBG cord-304685-s0bfhwtn 152 7 activity activity NN cord-304685-s0bfhwtn 152 8 was be VBD cord-304685-s0bfhwtn 152 9 localized localize VBN cord-304685-s0bfhwtn 152 10 to to IN cord-304685-s0bfhwtn 152 11 the the DT cord-304685-s0bfhwtn 152 12 HSV-1 HSV-1 NNP cord-304685-s0bfhwtn 152 13 IE IE NNP cord-304685-s0bfhwtn 152 14 protein protein NN cord-304685-s0bfhwtn 152 15 ICP0 ICP0 NNP cord-304685-s0bfhwtn 152 16 , , , cord-304685-s0bfhwtn 152 17 which which WDT cord-304685-s0bfhwtn 152 18 is be VBZ cord-304685-s0bfhwtn 152 19 rcqnired rcqnire VBN cord-304685-s0bfhwtn 152 20 for for IN cord-304685-s0bfhwtn 152 21 the the DT cord-304685-s0bfhwtn 152 22 expression expression NN cord-304685-s0bfhwtn 152 23 of of IN cord-304685-s0bfhwtn 152 24 IE IE NNP cord-304685-s0bfhwtn 152 25 proteins protein NNS cord-304685-s0bfhwtn 152 26 ICP4 ICP4 NNP cord-304685-s0bfhwtn 152 27 and and CC cord-304685-s0bfhwtn 152 28 ICP27 ICP27 NNP cord-304685-s0bfhwtn 152 29 ( ( -LRB- cord-304685-s0bfhwtn 152 30 30 30 CD cord-304685-s0bfhwtn 152 31 ) ) -RRB- cord-304685-s0bfhwtn 152 32 ( ( -LRB- cord-304685-s0bfhwtn 152 33 31 31 CD cord-304685-s0bfhwtn 152 34 ) ) -RRB- cord-304685-s0bfhwtn 152 35 ( ( -LRB- cord-304685-s0bfhwtn 152 36 32 32 CD cord-304685-s0bfhwtn 152 37 ) ) -RRB- cord-304685-s0bfhwtn 152 38 . . . cord-304685-s0bfhwtn 153 1 ICP4 ICP4 NNP cord-304685-s0bfhwtn 153 2 and and CC cord-304685-s0bfhwtn 153 3 ICP27 ICP27 NNP cord-304685-s0bfhwtn 153 4 have have VBP cord-304685-s0bfhwtn 153 5 been be VBN cord-304685-s0bfhwtn 153 6 defined define VBN cord-304685-s0bfhwtn 153 7 as as IN cord-304685-s0bfhwtn 153 8 containing contain VBG cord-304685-s0bfhwtn 153 9 immunodominant immunodominant JJ cord-304685-s0bfhwtn 153 10 CTL CTL NNP cord-304685-s0bfhwtn 153 11 epitopes epitope NNS cord-304685-s0bfhwtn 153 12 in in IN cord-304685-s0bfhwtn 153 13 BALB BALB NNP cord-304685-s0bfhwtn 153 14 / / SYM cord-304685-s0bfhwtn 153 15 c c NN cord-304685-s0bfhwtn 153 16 mice mouse NNS cord-304685-s0bfhwtn 153 17 ( ( -LRB- cord-304685-s0bfhwtn 153 18 33 33 CD cord-304685-s0bfhwtn 153 19 , , , cord-304685-s0bfhwtn 153 20 34 34 CD cord-304685-s0bfhwtn 153 21 ) ) -RRB- cord-304685-s0bfhwtn 153 22 . . . cord-304685-s0bfhwtn 154 1 It -PRON- PRP cord-304685-s0bfhwtn 154 2 is be VBZ cord-304685-s0bfhwtn 154 3 interesting interesting JJ cord-304685-s0bfhwtn 154 4 to to TO cord-304685-s0bfhwtn 154 5 note note VB cord-304685-s0bfhwtn 154 6 that that IN cord-304685-s0bfhwtn 154 7 immunodominant immunodominant NN cord-304685-s0bfhwtn 154 8 MHC MHC NNP cord-304685-s0bfhwtn 154 9 class class NN cord-304685-s0bfhwtn 154 10 I i NN cord-304685-s0bfhwtn 154 11 - - HYPH cord-304685-s0bfhwtn 154 12 restricted restrict VBN cord-304685-s0bfhwtn 154 13 CTL CTL NNP cord-304685-s0bfhwtn 154 14 epitopes epitope NNS cord-304685-s0bfhwtn 154 15 have have VBP cord-304685-s0bfhwtn 154 16 also also RB cord-304685-s0bfhwtn 154 17 been be VBN cord-304685-s0bfhwtn 154 18 identified identify VBN cord-304685-s0bfhwtn 154 19 in in IN cord-304685-s0bfhwtn 154 20 the the DT cord-304685-s0bfhwtn 154 21 IE IE NNP cord-304685-s0bfhwtn 154 22 gene gene NN cord-304685-s0bfhwtn 154 23 , , , cord-304685-s0bfhwtn 154 24 pp89 pp89 NNP cord-304685-s0bfhwtn 154 25 , , , cord-304685-s0bfhwtn 154 26 of of IN cord-304685-s0bfhwtn 154 27 murine murine JJ cord-304685-s0bfhwtn 154 28 cytomegalovirus cytomegalovirus NN cord-304685-s0bfhwtn 154 29 , , , cord-304685-s0bfhwtn 154 30 which which WDT cord-304685-s0bfhwtn 154 31 induces induce VBZ cord-304685-s0bfhwtn 154 32 c c NN cord-304685-s0bfhwtn 154 33 - - HYPH cord-304685-s0bfhwtn 154 34 fos fos NNP cord-304685-s0bfhwtn 154 35 expression expression NN cord-304685-s0bfhwtn 154 36 and and CC cord-304685-s0bfhwtn 154 37 cdlular cdlular JJ cord-304685-s0bfhwtn 154 38 DNA dna NN cord-304685-s0bfhwtn 154 39 synthesis synthesis NN cord-304685-s0bfhwtn 154 40 in in IN cord-304685-s0bfhwtn 154 41 host host NN cord-304685-s0bfhwtn 154 42 cells cell NNS cord-304685-s0bfhwtn 154 43 ( ( -LRB- cord-304685-s0bfhwtn 154 44 35 35 CD cord-304685-s0bfhwtn 154 45 ) ) -RRB- cord-304685-s0bfhwtn 154 46 . . . cord-304685-s0bfhwtn 155 1 Thus thus RB cord-304685-s0bfhwtn 155 2 , , , cord-304685-s0bfhwtn 155 3 DNA dna NN cord-304685-s0bfhwtn 155 4 viruses virus NNS cord-304685-s0bfhwtn 155 5 induce induce VBP cord-304685-s0bfhwtn 155 6 cdlular cdlular JJ cord-304685-s0bfhwtn 155 7 activation activation NN cord-304685-s0bfhwtn 155 8 and and CC cord-304685-s0bfhwtn 155 9 replication replication NN cord-304685-s0bfhwtn 155 10 competence competence NN cord-304685-s0bfhwtn 155 11 through through IN cord-304685-s0bfhwtn 155 12 protooncogene protooncogene JJ cord-304685-s0bfhwtn 155 13 expression expression NN cord-304685-s0bfhwtn 155 14 , , , cord-304685-s0bfhwtn 155 15 and and CC cord-304685-s0bfhwtn 155 16 DNA dna NN cord-304685-s0bfhwtn 155 17 viral viral JJ cord-304685-s0bfhwtn 155 18 proteins protein NNS cord-304685-s0bfhwtn 155 19 synthesized synthesize VBN cord-304685-s0bfhwtn 156 1 early early RB cord-304685-s0bfhwtn 156 2 after after IN cord-304685-s0bfhwtn 156 3 infection infection NN cord-304685-s0bfhwtn 156 4 may may MD cord-304685-s0bfhwtn 156 5 both both DT cord-304685-s0bfhwtn 156 6 induce induce VB cord-304685-s0bfhwtn 156 7 cellular cellular JJ cord-304685-s0bfhwtn 156 8 activation activation NN cord-304685-s0bfhwtn 156 9 through through IN cord-304685-s0bfhwtn 156 10 protooncogene protooncogene JJ cord-304685-s0bfhwtn 156 11 expression expression NN cord-304685-s0bfhwtn 156 12 and and CC cord-304685-s0bfhwtn 156 13 provide provide VB cord-304685-s0bfhwtn 156 14 CTL CTL NNP cord-304685-s0bfhwtn 156 15 epitopes epitope NNS cord-304685-s0bfhwtn 156 16 to to TO cord-304685-s0bfhwtn 156 17 render render VB cord-304685-s0bfhwtn 156 18 these these DT cord-304685-s0bfhwtn 156 19 unwanted unwanted JJ cord-304685-s0bfhwtn 156 20 cells cell NNS cord-304685-s0bfhwtn 156 21 susceptible susceptible JJ cord-304685-s0bfhwtn 156 22 to to IN cord-304685-s0bfhwtn 156 23 the the DT cord-304685-s0bfhwtn 156 24 full full JJ cord-304685-s0bfhwtn 156 25 CTL CTL NNP cord-304685-s0bfhwtn 156 26 armament armament NN cord-304685-s0bfhwtn 156 27 . . . cord-304685-s0bfhwtn 157 1 The the DT cord-304685-s0bfhwtn 157 2 earty earty NNP cord-304685-s0bfhwtn 157 3 recognition recognition NN cord-304685-s0bfhwtn 157 4 of of IN cord-304685-s0bfhwtn 157 5 the the DT cord-304685-s0bfhwtn 157 6 virus virus NN cord-304685-s0bfhwtn 157 7 - - HYPH cord-304685-s0bfhwtn 157 8 infected infect VBN cord-304685-s0bfhwtn 157 9 cell cell NN cord-304685-s0bfhwtn 157 10 by by IN cord-304685-s0bfhwtn 157 11 specific specific JJ cord-304685-s0bfhwtn 157 12 CTL CTL NNP cord-304685-s0bfhwtn 157 13 could could MD cord-304685-s0bfhwtn 157 14 also also RB cord-304685-s0bfhwtn 157 15 allow allow VB cord-304685-s0bfhwtn 157 16 for for IN cord-304685-s0bfhwtn 157 17 the the DT cord-304685-s0bfhwtn 157 18 degradation degradation NN cord-304685-s0bfhwtn 157 19 of of IN cord-304685-s0bfhwtn 157 20 viral viral JJ cord-304685-s0bfhwtn 157 21 DNA dna NN cord-304685-s0bfhwtn 157 22 before before IN cord-304685-s0bfhwtn 157 23 viral viral JJ cord-304685-s0bfhwtn 157 24 packaging packaging NN cord-304685-s0bfhwtn 157 25 and and CC cord-304685-s0bfhwtn 157 26 release release NN cord-304685-s0bfhwtn 157 27 . . . cord-304685-s0bfhwtn 158 1 Cell cell NN cord-304685-s0bfhwtn 158 2 death death NN cord-304685-s0bfhwtn 158 3 by by IN cord-304685-s0bfhwtn 158 4 apoptosis apoptosis NN cord-304685-s0bfhwtn 158 5 is be VBZ cord-304685-s0bfhwtn 158 6 irreversible irreversible JJ cord-304685-s0bfhwtn 158 7 , , , cord-304685-s0bfhwtn 158 8 but but CC cord-304685-s0bfhwtn 158 9 purified purify VBN cord-304685-s0bfhwtn 158 10 perforin perforin NNP cord-304685-s0bfhwtn 158 11 has have VBZ cord-304685-s0bfhwtn 158 12 been be VBN cord-304685-s0bfhwtn 158 13 shown show VBN cord-304685-s0bfhwtn 158 14 to to TO cord-304685-s0bfhwtn 158 15 induce induce VB cord-304685-s0bfhwtn 158 16 a a DT cord-304685-s0bfhwtn 158 17 transient transient JJ cord-304685-s0bfhwtn 158 18 membrane membrane NN cord-304685-s0bfhwtn 158 19 permeability permeability NN cord-304685-s0bfhwtn 158 20 that that WDT cord-304685-s0bfhwtn 158 21 can can MD cord-304685-s0bfhwtn 158 22 be be VB cord-304685-s0bfhwtn 158 23 repaired repair VBN cord-304685-s0bfhwtn 158 24 ( ( -LRB- cord-304685-s0bfhwtn 158 25 36 36 CD cord-304685-s0bfhwtn 158 26 ) ) -RRB- cord-304685-s0bfhwtn 158 27 . . . cord-304685-s0bfhwtn 159 1 Thus thus RB cord-304685-s0bfhwtn 159 2 , , , cord-304685-s0bfhwtn 159 3 the the DT cord-304685-s0bfhwtn 159 4 membrane membrane NN cord-304685-s0bfhwtn 159 5 lysis lysis NN cord-304685-s0bfhwtn 159 6 mechanism mechanism NN cord-304685-s0bfhwtn 159 7 of of IN cord-304685-s0bfhwtn 159 8 CTL CTL NNP cord-304685-s0bfhwtn 159 9 may may MD cord-304685-s0bfhwtn 159 10 be be VB cord-304685-s0bfhwtn 159 11 less less RBR cord-304685-s0bfhwtn 159 12 efficient efficient JJ cord-304685-s0bfhwtn 159 13 than than IN cord-304685-s0bfhwtn 159 14 the the DT cord-304685-s0bfhwtn 159 15 apoptosis apoptosis NN cord-304685-s0bfhwtn 159 16 mechanism mechanism NN cord-304685-s0bfhwtn 159 17 for for IN cord-304685-s0bfhwtn 159 18 irreversible irreversible JJ cord-304685-s0bfhwtn 159 19 killing killing NN cord-304685-s0bfhwtn 159 20 of of IN cord-304685-s0bfhwtn 159 21 a a DT cord-304685-s0bfhwtn 159 22 target target NN cord-304685-s0bfhwtn 159 23 , , , cord-304685-s0bfhwtn 159 24 and and CC cord-304685-s0bfhwtn 159 25 the the DT cord-304685-s0bfhwtn 159 26 induction induction NN cord-304685-s0bfhwtn 159 27 of of IN cord-304685-s0bfhwtn 159 28 competence competence NN cord-304685-s0bfhwtn 159 29 for for IN cord-304685-s0bfhwtn 159 30 CTLinduced ctlinduce VBN cord-304685-s0bfhwtn 159 31 apoptosis apoptosis NN cord-304685-s0bfhwtn 159 32 may may MD cord-304685-s0bfhwtn 159 33 therefore therefore RB cord-304685-s0bfhwtn 159 34 be be VB cord-304685-s0bfhwtn 159 35 of of IN cord-304685-s0bfhwtn 159 36 pathological pathological JJ cord-304685-s0bfhwtn 159 37 significance significance NN cord-304685-s0bfhwtn 159 38 . . . cord-304685-s0bfhwtn 160 1 MHC MHC NNP cord-304685-s0bfhwtn 160 2 - - HYPH cord-304685-s0bfhwtn 160 3 restricted restrict VBN cord-304685-s0bfhwtn 160 4 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 160 5 T t NN cord-304685-s0bfhwtn 160 6 cells cell NNS cord-304685-s0bfhwtn 160 7 : : : cord-304685-s0bfhwtn 161 1 studies study NNS cord-304685-s0bfhwtn 161 2 on on IN cord-304685-s0bfhwtn 161 3 the the DT cord-304685-s0bfhwtn 161 4 biological biological JJ cord-304685-s0bfhwtn 161 5 role role NN cord-304685-s0bfhwtn 161 6 of of IN cord-304685-s0bfhwtn 161 7 polymorphic polymorphic JJ cord-304685-s0bfhwtn 161 8 major major JJ cord-304685-s0bfhwtn 161 9 transplantation transplantation NN cord-304685-s0bfhwtn 161 10 antigens antigen NNS cord-304685-s0bfhwtn 161 11 determine determine VBP cord-304685-s0bfhwtn 161 12 T t NN cord-304685-s0bfhwtn 161 13 - - HYPH cord-304685-s0bfhwtn 161 14 cell cell NN cord-304685-s0bfhwtn 161 15 restrictionspecificity restrictionspecificity NN cord-304685-s0bfhwtn 161 16 , , , cord-304685-s0bfhwtn 161 17 function function NN cord-304685-s0bfhwtn 161 18 , , , cord-304685-s0bfhwtn 161 19 and and CC cord-304685-s0bfhwtn 161 20 responsiveness responsiveness NN cord-304685-s0bfhwtn 161 21 Ceil Ceil NNP cord-304685-s0bfhwtn 161 22 - - HYPH cord-304685-s0bfhwtn 161 23 mediated mediate VBN cord-304685-s0bfhwtn 161 24 killing killing NN cord-304685-s0bfhwtn 161 25 : : : cord-304685-s0bfhwtn 161 26 effector effector NN cord-304685-s0bfhwtn 161 27 mechanisms mechanism NNS cord-304685-s0bfhwtn 161 28 and and CC cord-304685-s0bfhwtn 161 29 mediators mediator NNS cord-304685-s0bfhwtn 162 1 Purified purify VBN cord-304685-s0bfhwtn 162 2 perforin perforin NNP cord-304685-s0bfhwtn 162 3 induces induce VBZ cord-304685-s0bfhwtn 162 4 target target NN cord-304685-s0bfhwtn 162 5 cell cell NN cord-304685-s0bfhwtn 162 6 lysis lysis NN cord-304685-s0bfhwtn 162 7 but but CC cord-304685-s0bfhwtn 162 8 not not RB cord-304685-s0bfhwtn 162 9 DNA dna NN cord-304685-s0bfhwtn 162 10 fragmentation fragmentation NN cord-304685-s0bfhwtn 162 11 , , , cord-304685-s0bfhwtn 162 12 f f NNP cord-304685-s0bfhwtn 162 13 Ex Ex NNP cord-304685-s0bfhwtn 162 14 F F NNP cord-304685-s0bfhwtn 162 15 Med Med NNP cord-304685-s0bfhwtn 162 16 Cytotoxicity Cytotoxicity NNP cord-304685-s0bfhwtn 162 17 with with IN cord-304685-s0bfhwtn 162 18 target target NN cord-304685-s0bfhwtn 162 19 DNA dna NN cord-304685-s0bfhwtn 162 20 breakdown breakdown NN cord-304685-s0bfhwtn 162 21 by by IN cord-304685-s0bfhwtn 162 22 rat rat NNP cord-304685-s0bfhwtn 162 23 basophilic basophilic NNP cord-304685-s0bfhwtn 162 24 leukemia leukemia NNP cord-304685-s0bfhwtn 162 25 ceils ceils NNP cord-304685-s0bfhwtn 162 26 expressing express VBG cord-304685-s0bfhwtn 162 27 both both DT cord-304685-s0bfhwtn 162 28 cytolysin cytolysin NNS cord-304685-s0bfhwtn 162 29 and and CC cord-304685-s0bfhwtn 162 30 granzyme granzyme NNS cord-304685-s0bfhwtn 163 1 A a DT cord-304685-s0bfhwtn 163 2 Inhibition inhibition NN cord-304685-s0bfhwtn 163 3 of of IN cord-304685-s0bfhwtn 163 4 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 163 5 T t NN cord-304685-s0bfhwtn 163 6 lymphocyte lymphocyte NN cord-304685-s0bfhwtn 163 7 - - HYPH cord-304685-s0bfhwtn 163 8 induced induce VBN cord-304685-s0bfhwtn 163 9 target target NN cord-304685-s0bfhwtn 163 10 ceil ceil NN cord-304685-s0bfhwtn 163 11 DNA dna NN cord-304685-s0bfhwtn 163 12 fragmentation fragmentation NN cord-304685-s0bfhwtn 163 13 , , , cord-304685-s0bfhwtn 163 14 but but CC cord-304685-s0bfhwtn 163 15 not not RB cord-304685-s0bfhwtn 163 16 lysis lysis NN cord-304685-s0bfhwtn 163 17 , , , cord-304685-s0bfhwtn 163 18 by by IN cord-304685-s0bfhwtn 163 19 inhibitors inhibitor NNS cord-304685-s0bfhwtn 163 20 of of IN cord-304685-s0bfhwtn 163 21 DNA dna NN cord-304685-s0bfhwtn 163 22 topoisomerases topoisomerase NNS cord-304685-s0bfhwtn 163 23 I -PRON- PRP cord-304685-s0bfhwtn 163 24 and and CC cord-304685-s0bfhwtn 163 25 II II NNP cord-304685-s0bfhwtn 163 26 . . . cord-304685-s0bfhwtn 164 1 f f NNP cord-304685-s0bfhwtn 164 2 Ex Ex NNP cord-304685-s0bfhwtn 164 3 F F NNP cord-304685-s0bfhwtn 164 4 Med Med NNP cord-304685-s0bfhwtn 164 5 Differential Differential NNP cord-304685-s0bfhwtn 164 6 expression expression NN cord-304685-s0bfhwtn 164 7 of of IN cord-304685-s0bfhwtn 164 8 DNA dna NN cord-304685-s0bfhwtn 164 9 topoisomerases topoisomerase NNS cord-304685-s0bfhwtn 164 10 I -PRON- PRP cord-304685-s0bfhwtn 164 11 and and CC cord-304685-s0bfhwtn 164 12 II II NNP cord-304685-s0bfhwtn 164 13 during during IN cord-304685-s0bfhwtn 164 14 the the DT cord-304685-s0bfhwtn 164 15 eukaryotic eukaryotic JJ cord-304685-s0bfhwtn 164 16 cell cell NN cord-304685-s0bfhwtn 164 17 cycle cycle NN cord-304685-s0bfhwtn 164 18 Mechanism mechanism NN cord-304685-s0bfhwtn 164 19 of of IN cord-304685-s0bfhwtn 164 20 killing kill VBG cord-304685-s0bfhwtn 164 21 by by IN cord-304685-s0bfhwtn 164 22 virus virus NN cord-304685-s0bfhwtn 164 23 - - HYPH cord-304685-s0bfhwtn 164 24 induced induce VBN cord-304685-s0bfhwtn 164 25 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 164 26 T t NN cord-304685-s0bfhwtn 164 27 lymphocytes lymphocyte NNS cord-304685-s0bfhwtn 164 28 elicited elicit VBN cord-304685-s0bfhwtn 164 29 in in IN cord-304685-s0bfhwtn 164 30 vivo vivo NN cord-304685-s0bfhwtn 164 31 . . . cord-304685-s0bfhwtn 165 1 f f NNP cord-304685-s0bfhwtn 166 1 Virol Virol NNP cord-304685-s0bfhwtn 166 2 Quantitative quantitative JJ cord-304685-s0bfhwtn 166 3 studies study NNS cord-304685-s0bfhwtn 166 4 of of IN cord-304685-s0bfhwtn 166 5 amino amino NN cord-304685-s0bfhwtn 166 6 acid acid NN cord-304685-s0bfhwtn 166 7 and and CC cord-304685-s0bfhwtn 166 8 growth growth NN cord-304685-s0bfhwtn 166 9 factor factor NN cord-304685-s0bfhwtn 166 10 requirements requirement NNS cord-304685-s0bfhwtn 166 11 of of IN cord-304685-s0bfhwtn 166 12 transformed transform VBN cord-304685-s0bfhwtn 166 13 and and CC cord-304685-s0bfhwtn 166 14 nontransformed nontransforme VBN cord-304685-s0bfhwtn 166 15 cells cell NNS cord-304685-s0bfhwtn 166 16 in in IN cord-304685-s0bfhwtn 166 17 high high JJ cord-304685-s0bfhwtn 166 18 concentrations concentration NNS cord-304685-s0bfhwtn 166 19 of of IN cord-304685-s0bfhwtn 166 20 serum serum NN cord-304685-s0bfhwtn 166 21 or or CC cord-304685-s0bfhwtn 166 22 lymph lymph NN cord-304685-s0bfhwtn 166 23 CTL CTL NNP cord-304685-s0bfhwtn 166 24 : : : cord-304685-s0bfhwtn 166 25 virus virus NN cord-304685-s0bfhwtn 166 26 control control NNP cord-304685-s0bfhwtn 166 27 ceils ceils NNP cord-304685-s0bfhwtn 166 28 first first RB cord-304685-s0bfhwtn 166 29 and and CC cord-304685-s0bfhwtn 166 30 cytolytic cytolytic JJ cord-304685-s0bfhwtn 166 31 cells cell NNS cord-304685-s0bfhwtn 166 32 second second RB cord-304685-s0bfhwtn 166 33 ? ? . cord-304685-s0bfhwtn 167 1 DNA dna NN cord-304685-s0bfhwtn 167 2 fragmentation fragmentation NN cord-304685-s0bfhwtn 167 3 , , , cord-304685-s0bfhwtn 167 4 apoptosis apoptosis NN cord-304685-s0bfhwtn 167 5 and and CC cord-304685-s0bfhwtn 167 6 the the DT cord-304685-s0bfhwtn 167 7 prelytic prelytic JJ cord-304685-s0bfhwtn 167 8 halt halt NN cord-304685-s0bfhwtn 167 9 hypothesis hypothesis NN cord-304685-s0bfhwtn 168 1 Mutations mutation NNS cord-304685-s0bfhwtn 168 2 in in IN cord-304685-s0bfhwtn 168 3 the the DT cord-304685-s0bfhwtn 168 4 thymidine thymidine NN cord-304685-s0bfhwtn 168 5 kinase kinase NN cord-304685-s0bfhwtn 168 6 gene gene NN cord-304685-s0bfhwtn 168 7 that that WDT cord-304685-s0bfhwtn 168 8 allow allow VBP cord-304685-s0bfhwtn 168 9 expression expression NN cord-304685-s0bfhwtn 168 10 of of IN cord-304685-s0bfhwtn 168 11 the the DT cord-304685-s0bfhwtn 168 12 enzyme enzyme NN cord-304685-s0bfhwtn 168 13 in in IN cord-304685-s0bfhwtn 168 14 quiescent quiescent JJ cord-304685-s0bfhwtn 168 15 ( ( -LRB- cord-304685-s0bfhwtn 168 16 GO GO NNP cord-304685-s0bfhwtn 168 17 ) ) -RRB- cord-304685-s0bfhwtn 168 18 cells cell NNS cord-304685-s0bfhwtn 168 19 The the DT cord-304685-s0bfhwtn 168 20 3T3 3t3 CD cord-304685-s0bfhwtn 168 21 cell cell NN cord-304685-s0bfhwtn 168 22 cycle cycle NN cord-304685-s0bfhwtn 168 23 at at IN cord-304685-s0bfhwtn 168 24 low low JJ cord-304685-s0bfhwtn 168 25 proliferation proliferation NN cord-304685-s0bfhwtn 168 26 rates rate NNS cord-304685-s0bfhwtn 168 27 Oncogenes oncogene NNS cord-304685-s0bfhwtn 168 28 , , , cord-304685-s0bfhwtn 168 29 growth growth NN cord-304685-s0bfhwtn 168 30 , , , cord-304685-s0bfhwtn 168 31 and and CC cord-304685-s0bfhwtn 168 32 the the DT cord-304685-s0bfhwtn 168 33 cell cell NN cord-304685-s0bfhwtn 168 34 cycle cycle NN cord-304685-s0bfhwtn 168 35 : : : cord-304685-s0bfhwtn 169 1 an an DT cord-304685-s0bfhwtn 169 2 overview overview NN cord-304685-s0bfhwtn 169 3 Quail Quail NNP cord-304685-s0bfhwtn 169 4 embryo embryo NN cord-304685-s0bfhwtn 169 5 fibroblasts fibroblast NNS cord-304685-s0bfhwtn 169 6 transformed transform VBN cord-304685-s0bfhwtn 169 7 by by IN cord-304685-s0bfhwtn 169 8 four four CD cord-304685-s0bfhwtn 169 9 v v NN cord-304685-s0bfhwtn 169 10 - - HYPH cord-304685-s0bfhwtn 169 11 myc myc NN cord-304685-s0bfhwtn 169 12 - - HYPH cord-304685-s0bfhwtn 169 13 containing contain VBG cord-304685-s0bfhwtn 170 1 virus virus NNP cord-304685-s0bfhwtn 170 2 isolates isolate NNS cord-304685-s0bfhwtn 170 3 show show VBP cord-304685-s0bfhwtn 170 4 enhanced enhanced JJ cord-304685-s0bfhwtn 170 5 proliferation proliferation NN cord-304685-s0bfhwtn 170 6 but but CC cord-304685-s0bfhwtn 170 7 are be VBP cord-304685-s0bfhwtn 170 8 nontumorigenic nontumorigenic JJ cord-304685-s0bfhwtn 170 9 G1 G1 NNP cord-304685-s0bfhwtn 170 10 events event NNS cord-304685-s0bfhwtn 170 11 and and CC cord-304685-s0bfhwtn 170 12 regulation regulation NN cord-304685-s0bfhwtn 170 13 of of IN cord-304685-s0bfhwtn 170 14 cell cell NN cord-304685-s0bfhwtn 170 15 proliferation proliferation NN cord-304685-s0bfhwtn 171 1 Bcl-2 Bcl-2 NNP cord-304685-s0bfhwtn 171 2 is be VBZ cord-304685-s0bfhwtn 171 3 an an DT cord-304685-s0bfhwtn 171 4 inner inner JJ cord-304685-s0bfhwtn 171 5 mitochondrial mitochondrial NN cord-304685-s0bfhwtn 171 6 membrane membrane NN cord-304685-s0bfhwtn 171 7 protein protein NN cord-304685-s0bfhwtn 171 8 that that WDT cord-304685-s0bfhwtn 171 9 blocks block VBZ cord-304685-s0bfhwtn 171 10 programmed program VBN cord-304685-s0bfhwtn 171 11 cell cell NN cord-304685-s0bfhwtn 171 12 death death NN cord-304685-s0bfhwtn 171 13 Ceil Ceil NNP cord-304685-s0bfhwtn 171 14 cycle cycle NN cord-304685-s0bfhwtn 171 15 analysis analysis NN cord-304685-s0bfhwtn 171 16 of of IN cord-304685-s0bfhwtn 171 17 p53-induced p53-induced JJ cord-304685-s0bfhwtn 171 18 ceil ceil NN cord-304685-s0bfhwtn 171 19 death death NN cord-304685-s0bfhwtn 171 20 in in IN cord-304685-s0bfhwtn 171 21 routine routine JJ cord-304685-s0bfhwtn 171 22 erythroleukemia erythroleukemia NN cord-304685-s0bfhwtn 171 23 cells cell NNS cord-304685-s0bfhwtn 172 1 A a DT cord-304685-s0bfhwtn 172 2 mouse mouse NN cord-304685-s0bfhwtn 172 3 c c NNP cord-304685-s0bfhwtn 172 4 - - HYPH cord-304685-s0bfhwtn 172 5 myc myc NNP cord-304685-s0bfhwtn 172 6 retrovirus retrovirus NN cord-304685-s0bfhwtn 172 7 transforms transform VBZ cord-304685-s0bfhwtn 172 8 established establish VBN cord-304685-s0bfhwtn 172 9 fibroblast fibroblast NN cord-304685-s0bfhwtn 172 10 lines line NNS cord-304685-s0bfhwtn 172 11 in in IN cord-304685-s0bfhwtn 172 12 vitro vitro FW cord-304685-s0bfhwtn 172 13 and and CC cord-304685-s0bfhwtn 172 14 induces induce VBZ cord-304685-s0bfhwtn 172 15 monocyte monocyte JJ cord-304685-s0bfhwtn 172 16 - - HYPH cord-304685-s0bfhwtn 172 17 macrophage macrophage NN cord-304685-s0bfhwtn 172 18 tumors tumor NNS cord-304685-s0bfhwtn 172 19 in in IN cord-304685-s0bfhwtn 172 20 vivo vivo NNP cord-304685-s0bfhwtn 172 21 Herpes Herpes NNP cord-304685-s0bfhwtn 172 22 simplex simplex NN cord-304685-s0bfhwtn 172 23 virus virus NN cord-304685-s0bfhwtn 172 24 type type NN cord-304685-s0bfhwtn 172 25 2 2 CD cord-304685-s0bfhwtn 172 26 functions function NNS cord-304685-s0bfhwtn 172 27 expressed express VBN cord-304685-s0bfhwtn 172 28 during during IN cord-304685-s0bfhwtn 172 29 stimulation stimulation NN cord-304685-s0bfhwtn 172 30 of of IN cord-304685-s0bfhwtn 172 31 human human JJ cord-304685-s0bfhwtn 172 32 cell cell NN cord-304685-s0bfhwtn 172 33 DNA dna NN cord-304685-s0bfhwtn 172 34 synthesis synthesis NN cord-304685-s0bfhwtn 172 35 Human Human NNP cord-304685-s0bfhwtn 172 36 cytomegalovirus cytomegalovirus NN cord-304685-s0bfhwtn 172 37 induces induce VBZ cord-304685-s0bfhwtn 172 38 expression expression NN cord-304685-s0bfhwtn 172 39 of of IN cord-304685-s0bfhwtn 172 40 cellular cellular JJ cord-304685-s0bfhwtn 172 41 topoisomerase topoisomerase NN cord-304685-s0bfhwtn 172 42 II II NNP cord-304685-s0bfhwtn 172 43 Topoisomerase Topoisomerase NNP cord-304685-s0bfhwtn 172 44 II II NNP cord-304685-s0bfhwtn 172 45 cleavage cleavage NN cord-304685-s0bfhwtn 172 46 of of IN cord-304685-s0bfhwtn 172 47 herpes herpes NN cord-304685-s0bfhwtn 172 48 simplex simplex NNP cord-304685-s0bfhwtn 172 49 virus virus NN cord-304685-s0bfhwtn 172 50 type type NN cord-304685-s0bfhwtn 172 51 1 1 CD cord-304685-s0bfhwtn 172 52 DNA dna NN cord-304685-s0bfhwtn 172 53 in in IN cord-304685-s0bfhwtn 172 54 vivo vivo NN cord-304685-s0bfhwtn 172 55 is be VBZ cord-304685-s0bfhwtn 172 56 replication replication NN cord-304685-s0bfhwtn 172 57 dependent dependent JJ cord-304685-s0bfhwtn 172 58 Role role NN cord-304685-s0bfhwtn 173 1 ofDNA ofdna RB cord-304685-s0bfhwtn 174 1 topoisomerase topoisomerase NNP cord-304685-s0bfhwtn 175 1 I -PRON- PRP cord-304685-s0bfhwtn 175 2 in in IN cord-304685-s0bfhwtn 175 3 the the DT cord-304685-s0bfhwtn 175 4 replication replication NN cord-304685-s0bfhwtn 175 5 of of IN cord-304685-s0bfhwtn 175 6 herpes herpes NNP cord-304685-s0bfhwtn 175 7 simplex simplex NNP cord-304685-s0bfhwtn 175 8 virus virus NN cord-304685-s0bfhwtn 175 9 type type NN cord-304685-s0bfhwtn 175 10 2 2 CD cord-304685-s0bfhwtn 175 11 Human human JJ cord-304685-s0bfhwtn 175 12 tumor tumor NN cord-304685-s0bfhwtn 175 13 necrosis necrosis NN cord-304685-s0bfhwtn 175 14 factoralpha factoralpha NNP cord-304685-s0bfhwtn 175 15 kills kill VBZ cord-304685-s0bfhwtn 175 16 herpesvirus herpesvirus NN cord-304685-s0bfhwtn 175 17 - - HYPH cord-304685-s0bfhwtn 175 18 infected infect VBN cord-304685-s0bfhwtn 175 19 but but CC cord-304685-s0bfhwtn 175 20 not not RB cord-304685-s0bfhwtn 175 21 normal normal JJ cord-304685-s0bfhwtn 175 22 cells cell NNS cord-304685-s0bfhwtn 175 23 . . . cord-304685-s0bfhwtn 176 1 L),mphoki~ L),mphoki~ NNP cord-304685-s0bfhwtn 176 2 Res Res NNP cord-304685-s0bfhwtn 176 3 Anatomy Anatomy NNP cord-304685-s0bfhwtn 176 4 of of IN cord-304685-s0bfhwtn 176 5 herpes herpes NNP cord-304685-s0bfhwtn 176 6 simplex simplex NNP cord-304685-s0bfhwtn 176 7 virus virus NN cord-304685-s0bfhwtn 176 8 DNA DNA NNP cord-304685-s0bfhwtn 176 9 . . . cord-304685-s0bfhwtn 177 1 XI XI NNP cord-304685-s0bfhwtn 177 2 . . . cord-304685-s0bfhwtn 178 1 Apparent apparent JJ cord-304685-s0bfhwtn 178 2 clustering clustering NN cord-304685-s0bfhwtn 178 3 of of IN cord-304685-s0bfhwtn 178 4 functions function NNS cord-304685-s0bfhwtn 178 5 effecting effect VBG cord-304685-s0bfhwtn 178 6 rapid rapid JJ cord-304685-s0bfhwtn 178 7 inhibition inhibition NN cord-304685-s0bfhwtn 178 8 of of IN cord-304685-s0bfhwtn 178 9 host host NN cord-304685-s0bfhwtn 178 10 DNA dna NN cord-304685-s0bfhwtn 178 11 and and CC cord-304685-s0bfhwtn 178 12 protein protein NN cord-304685-s0bfhwtn 178 13 synthesis synthesis NN cord-304685-s0bfhwtn 178 14 Cytotoxicity Cytotoxicity NNP cord-304685-s0bfhwtn 178 15 of of IN cord-304685-s0bfhwtn 178 16 a a DT cord-304685-s0bfhwtn 178 17 replication replication NN cord-304685-s0bfhwtn 178 18 - - HYPH cord-304685-s0bfhwtn 178 19 defective defective JJ cord-304685-s0bfhwtn 178 20 mutant mutant NN cord-304685-s0bfhwtn 178 21 of of IN cord-304685-s0bfhwtn 178 22 herpes herpes NNP cord-304685-s0bfhwtn 178 23 simplex simplex NNP cord-304685-s0bfhwtn 178 24 virus virus NN cord-304685-s0bfhwtn 178 25 type type NN cord-304685-s0bfhwtn 178 26 1 1 CD cord-304685-s0bfhwtn 178 27 Vaccinia Vaccinia NNP cord-304685-s0bfhwtn 178 28 virus virus NN cord-304685-s0bfhwtn 178 29 encapsulates encapsulate VBZ cord-304685-s0bfhwtn 178 30 a a DT cord-304685-s0bfhwtn 178 31 novel novel JJ cord-304685-s0bfhwtn 178 32 topoisomerase topoisomerase NN cord-304685-s0bfhwtn 178 33 with with IN cord-304685-s0bfhwtn 178 34 the the DT cord-304685-s0bfhwtn 178 35 properties property NNS cord-304685-s0bfhwtn 178 36 of of IN cord-304685-s0bfhwtn 178 37 a a DT cord-304685-s0bfhwtn 178 38 eucaryotic eucaryotic JJ cord-304685-s0bfhwtn 178 39 type type NN cord-304685-s0bfhwtn 178 40 I -PRON- PRP cord-304685-s0bfhwtn 178 41 , , , cord-304685-s0bfhwtn 178 42 enzyme enzyme NN cord-304685-s0bfhwtn 178 43 Role role NN cord-304685-s0bfhwtn 178 44 for for IN cord-304685-s0bfhwtn 178 45 c c NNP cord-304685-s0bfhwtn 178 46 - - HYPH cord-304685-s0bfhwtn 178 47 myc myc NNP cord-304685-s0bfhwtn 178 48 in in IN cord-304685-s0bfhwtn 178 49 activationinduced activationinduced JJ cord-304685-s0bfhwtn 178 50 apoptotic apoptotic JJ cord-304685-s0bfhwtn 178 51 cell cell NN cord-304685-s0bfhwtn 178 52 death death NN cord-304685-s0bfhwtn 178 53 in in IN cord-304685-s0bfhwtn 178 54 T T NNP cord-304685-s0bfhwtn 178 55 cell cell NN cord-304685-s0bfhwtn 178 56 hybridomas hybridomas NN cord-304685-s0bfhwtn 179 1 A a DT cord-304685-s0bfhwtn 179 2 c c NNP cord-304685-s0bfhwtn 179 3 - - HYPH cord-304685-s0bfhwtn 179 4 myc myc NNP cord-304685-s0bfhwtn 179 5 antisense antisense NNP cord-304685-s0bfhwtn 179 6 oligodeoxynucleotide oligodeoxynucleotide NNP cord-304685-s0bfhwtn 179 7 inhibits inhibit VBZ cord-304685-s0bfhwtn 179 8 entry entry NN cord-304685-s0bfhwtn 179 9 into into IN cord-304685-s0bfhwtn 179 10 S s NN cord-304685-s0bfhwtn 179 11 phase phase NN cord-304685-s0bfhwtn 179 12 but but CC cord-304685-s0bfhwtn 179 13 not not RB cord-304685-s0bfhwtn 179 14 progress progress VB cord-304685-s0bfhwtn 179 15 from from IN cord-304685-s0bfhwtn 179 16 GO GO NNP cord-304685-s0bfhwtn 179 17 to to IN cord-304685-s0bfhwtn 179 18 G1 G1 NNP cord-304685-s0bfhwtn 179 19 Activation Activation NNP cord-304685-s0bfhwtn 179 20 of of IN cord-304685-s0bfhwtn 179 21 the the DT cord-304685-s0bfhwtn 179 22 cellular cellular JJ cord-304685-s0bfhwtn 179 23 transcription transcription NN cord-304685-s0bfhwtn 179 24 factor factor NN cord-304685-s0bfhwtn 180 1 AP-1 AP-1 NNP cord-304685-s0bfhwtn 181 1 in in IN cord-304685-s0bfhwtn 182 1 herpes herpes NNP cord-304685-s0bfhwtn 182 2 simplex simplex NNP cord-304685-s0bfhwtn 182 3 virus virus NN cord-304685-s0bfhwtn 182 4 infected infect VBD cord-304685-s0bfhwtn 182 5 ceils ceil NNS cord-304685-s0bfhwtn 182 6 is be VBZ cord-304685-s0bfhwtn 182 7 dependent dependent JJ cord-304685-s0bfhwtn 182 8 of of IN cord-304685-s0bfhwtn 182 9 the the DT cord-304685-s0bfhwtn 182 10 viral viral JJ cord-304685-s0bfhwtn 182 11 immediate immediate JJ cord-304685-s0bfhwtn 182 12 - - HYPH cord-304685-s0bfhwtn 182 13 early early JJ cord-304685-s0bfhwtn 182 14 protein protein NN cord-304685-s0bfhwtn 182 15 ICP0 ICP0 NNP cord-304685-s0bfhwtn 182 16 Existence Existence NNP cord-304685-s0bfhwtn 182 17 of of IN cord-304685-s0bfhwtn 182 18 different different JJ cord-304685-s0bfhwtn 182 19 Fos Fos NNP cord-304685-s0bfhwtn 182 20 / / SYM cord-304685-s0bfhwtn 182 21 Jun Jun NNP cord-304685-s0bfhwtn 182 22 complexes complex NNS cord-304685-s0bfhwtn 182 23 during during IN cord-304685-s0bfhwtn 182 24 the the DT cord-304685-s0bfhwtn 182 25 GO go NN cord-304685-s0bfhwtn 182 26 - - HYPH cord-304685-s0bfhwtn 182 27 to to IN cord-304685-s0bfhwtn 182 28 - - HYPH cord-304685-s0bfhwtn 182 29 G1 g1 NN cord-304685-s0bfhwtn 182 30 transition transition NN cord-304685-s0bfhwtn 182 31 and and CC cord-304685-s0bfhwtn 182 32 during during IN cord-304685-s0bfhwtn 182 33 exponential exponential JJ cord-304685-s0bfhwtn 182 34 growth growth NN cord-304685-s0bfhwtn 182 35 in in IN cord-304685-s0bfhwtn 182 36 mouse mouse NN cord-304685-s0bfhwtn 182 37 fibroblasts fibroblast NNS cord-304685-s0bfhwtn 182 38 : : : cord-304685-s0bfhwtn 182 39 differential differential JJ cord-304685-s0bfhwtn 182 40 role role NN cord-304685-s0bfhwtn 182 41 of of IN cord-304685-s0bfhwtn 182 42 Fos Fos NNP cord-304685-s0bfhwtn 182 43 proteins protein NNS cord-304685-s0bfhwtn 182 44 Herpes Herpes NNP cord-304685-s0bfhwtn 182 45 simplex simplex NN cord-304685-s0bfhwtn 182 46 virus virus NN cord-304685-s0bfhwtn 182 47 type type NN cord-304685-s0bfhwtn 182 48 1 1 CD cord-304685-s0bfhwtn 182 49 ICP0 ICP0 NNP cord-304685-s0bfhwtn 182 50 regulates regulate VBZ cord-304685-s0bfhwtn 182 51 expression expression NN cord-304685-s0bfhwtn 182 52 of of IN cord-304685-s0bfhwtn 182 53 immediate immediate JJ cord-304685-s0bfhwtn 182 54 - - HYPH cord-304685-s0bfhwtn 182 55 early early JJ cord-304685-s0bfhwtn 182 56 , , , cord-304685-s0bfhwtn 182 57 early early RB cord-304685-s0bfhwtn 182 58 , , , cord-304685-s0bfhwtn 182 59 and and CC cord-304685-s0bfhwtn 182 60 late late JJ cord-304685-s0bfhwtn 182 61 genes gene NNS cord-304685-s0bfhwtn 182 62 in in IN cord-304685-s0bfhwtn 182 63 productively productively RB cord-304685-s0bfhwtn 182 64 infected infect VBN cord-304685-s0bfhwtn 182 65 cells cell NNS cord-304685-s0bfhwtn 182 66 Association Association NNP cord-304685-s0bfhwtn 182 67 of of IN cord-304685-s0bfhwtn 182 68 ICP0 ICP0 NNP cord-304685-s0bfhwtn 183 1 but but CC cord-304685-s0bfhwtn 183 2 not not RB cord-304685-s0bfhwtn 183 3 ICP27 icp27 RB cord-304685-s0bfhwtn 183 4 with with IN cord-304685-s0bfhwtn 183 5 purified purify VBN cord-304685-s0bfhwtn 183 6 virions virion NNS cord-304685-s0bfhwtn 183 7 of of IN cord-304685-s0bfhwtn 183 8 herpes herpes NN cord-304685-s0bfhwtn 183 9 simplex simplex NNP cord-304685-s0bfhwtn 183 10 virus virus NN cord-304685-s0bfhwtn 183 11 type type NN cord-304685-s0bfhwtn 183 12 1 1 CD cord-304685-s0bfhwtn 183 13 . . . cord-304685-s0bfhwtn 184 1 f f LS cord-304685-s0bfhwtn 184 2 Virol Virol NNP cord-304685-s0bfhwtn 184 3 Herpes Herpes NNP cord-304685-s0bfhwtn 184 4 simplex simplex NN cord-304685-s0bfhwtn 184 5 viruses virus NNS cord-304685-s0bfhwtn 184 6 with with IN cord-304685-s0bfhwtn 184 7 mutations mutation NNS cord-304685-s0bfhwtn 184 8 in in IN cord-304685-s0bfhwtn 184 9 the the DT cord-304685-s0bfhwtn 184 10 gene gene NN cord-304685-s0bfhwtn 184 11 encoding encode VBG cord-304685-s0bfhwtn 184 12 ICP0 ICP0 NNP cord-304685-s0bfhwtn 184 13 are be VBP cord-304685-s0bfhwtn 184 14 defective defective JJ cord-304685-s0bfhwtn 184 15 in in IN cord-304685-s0bfhwtn 184 16 gene gene NN cord-304685-s0bfhwtn 184 17 expression expression NN cord-304685-s0bfhwtn 184 18 Recognition Recognition NNP cord-304685-s0bfhwtn 184 19 by by IN cord-304685-s0bfhwtn 184 20 an an DT cord-304685-s0bfhwtn 184 21 in in FW cord-304685-s0bfhwtn 184 22 vitro vitro FW cord-304685-s0bfhwtn 184 23 induction induction NN cord-304685-s0bfhwtn 184 24 of of IN cord-304685-s0bfhwtn 184 25 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 184 26 T t NN cord-304685-s0bfhwtn 184 27 lymphocytes lymphocyte NNS cord-304685-s0bfhwtn 184 28 against against IN cord-304685-s0bfhwtn 184 29 predicted predict VBN cord-304685-s0bfhwtn 184 30 epitopes epitope NNS cord-304685-s0bfhwtn 184 31 of of IN cord-304685-s0bfhwtn 184 32 the the DT cord-304685-s0bfhwtn 184 33 immediate immediate JJ cord-304685-s0bfhwtn 184 34 - - HYPH cord-304685-s0bfhwtn 184 35 early early JJ cord-304685-s0bfhwtn 184 36 protein protein NN cord-304685-s0bfhwtn 184 37 ICP27 ICP27 NNP cord-304685-s0bfhwtn 184 38 of of IN cord-304685-s0bfhwtn 184 39 herpes herpes NNP cord-304685-s0bfhwtn 184 40 simplex simplex NNP cord-304685-s0bfhwtn 184 41 virus virus NN cord-304685-s0bfhwtn 185 1 Murine murine JJ cord-304685-s0bfhwtn 185 2 cytotoxic cytotoxic JJ cord-304685-s0bfhwtn 185 3 T t NN cord-304685-s0bfhwtn 185 4 lymphocytes lymphocyte NNS cord-304685-s0bfhwtn 185 5 specific specific JJ cord-304685-s0bfhwtn 185 6 for for IN cord-304685-s0bfhwtn 185 7 herpes herpes NNP cord-304685-s0bfhwtn 185 8 simplex simplex NNP cord-304685-s0bfhwtn 185 9 virus virus NN cord-304685-s0bfhwtn 185 10 type type NN cord-304685-s0bfhwtn 185 11 1 1 CD cord-304685-s0bfhwtn 185 12 recognize recognize VBP cord-304685-s0bfhwtn 185 13 the the DT cord-304685-s0bfhwtn 185 14 immediate immediate JJ cord-304685-s0bfhwtn 185 15 early early JJ cord-304685-s0bfhwtn 185 16 protein protein NN cord-304685-s0bfhwtn 185 17 ICP4 ICP4 NNP cord-304685-s0bfhwtn 185 18 but but CC cord-304685-s0bfhwtn 185 19 not not RB cord-304685-s0bfhwtn 185 20 ICp0 icp0 NN cord-304685-s0bfhwtn 185 21 Cytolytic Cytolytic NNP cord-304685-s0bfhwtn 185 22 T T NNP cord-304685-s0bfhwtn 185 23 lymphocyte lymphocyte NN cord-304685-s0bfhwtn 185 24 recognition recognition NN cord-304685-s0bfhwtn 185 25 of of IN cord-304685-s0bfhwtn 185 26 the the DT cord-304685-s0bfhwtn 185 27 routine routine JJ cord-304685-s0bfhwtn 185 28 cytomegalovirus cytomegalovirus JJ cord-304685-s0bfhwtn 185 29 nonstructural nonstructural JJ cord-304685-s0bfhwtn 185 30 immediate immediate JJ cord-304685-s0bfhwtn 185 31 - - HYPH cord-304685-s0bfhwtn 185 32 early early JJ cord-304685-s0bfhwtn 185 33 prorein prorein NNS cord-304685-s0bfhwtn 185 34 pp89 pp89 NNP cord-304685-s0bfhwtn 185 35 expressed express VBN cord-304685-s0bfhwtn 185 36 by by IN cord-304685-s0bfhwtn 185 37 recombinant recombinant JJ cord-304685-s0bfhwtn 185 38 vaccinia vaccinia NN cord-304685-s0bfhwtn 185 39 virus virus NN cord-304685-s0bfhwtn 185 40 Reversible reversible JJ cord-304685-s0bfhwtn 185 41 cell cell NN cord-304685-s0bfhwtn 185 42 damage damage NN cord-304685-s0bfhwtn 185 43 by by IN cord-304685-s0bfhwtn 185 44 T t NN cord-304685-s0bfhwtn 185 45 - - HYPH cord-304685-s0bfhwtn 185 46 cell cell NN cord-304685-s0bfhwtn 185 47 perforins perforin NNS cord-304685-s0bfhwtn 185 48 . . . cord-304685-s0bfhwtn 186 1 Caldum Caldum NNP cord-304685-s0bfhwtn 186 2 influx influx NN cord-304685-s0bfhwtn 186 3 and and CC cord-304685-s0bfhwtn 186 4 propidium propidium JJ cord-304685-s0bfhwtn 186 5 iodide iodide NN cord-304685-s0bfhwtn 186 6 uptake uptake NN cord-304685-s0bfhwtn 186 7 into into IN cord-304685-s0bfhwtn 186 8 K562 k562 CD cord-304685-s0bfhwtn 186 9 cells cell NNS cord-304685-s0bfhwtn 186 10 in in IN cord-304685-s0bfhwtn 186 11 the the DT cord-304685-s0bfhwtn 186 12 absence absence NN cord-304685-s0bfhwtn 186 13 of of IN cord-304685-s0bfhwtn 186 14 lysis lysis NN