id	sid	tid	token	lemma	pos
cord-259738-yuqc6dk0	1	1	key	key	JJ
cord-259738-yuqc6dk0	1	2	:	:	:
cord-259738-yuqc6dk0	1	3	cord-259738-yuqc6dk0	cord-259738-yuqc6dk0	CD
cord-259738-yuqc6dk0	1	4	authors	author	NNS
cord-259738-yuqc6dk0	1	5	:	:	:
cord-259738-yuqc6dk0	1	6	Tang	Tang	NNP
cord-259738-yuqc6dk0	1	7	,	,	,
cord-259738-yuqc6dk0	1	8	Mengjun	Mengjun	NNP
cord-259738-yuqc6dk0	1	9	;	;	:
cord-259738-yuqc6dk0	1	10	Wang	Wang	NNP
cord-259738-yuqc6dk0	1	11	,	,	,
cord-259738-yuqc6dk0	1	12	Hongning	Hongning	NNP
cord-259738-yuqc6dk0	1	13	;	;	:
cord-259738-yuqc6dk0	1	14	Zhou	Zhou	NNP
cord-259738-yuqc6dk0	1	15	,	,	,
cord-259738-yuqc6dk0	1	16	Sheng	Sheng	NNP
cord-259738-yuqc6dk0	1	17	;	;	:
cord-259738-yuqc6dk0	1	18	Tian	Tian	NNP
cord-259738-yuqc6dk0	1	19	,	,	,
cord-259738-yuqc6dk0	1	20	Guobao	Guobao	NNP
cord-259738-yuqc6dk0	1	21	title	title	NN
cord-259738-yuqc6dk0	1	22	:	:	:
cord-259738-yuqc6dk0	1	23	Enhancement	enhancement	NN
cord-259738-yuqc6dk0	1	24	of	of	IN
cord-259738-yuqc6dk0	1	25	the	the	DT
cord-259738-yuqc6dk0	1	26	immunogenicity	immunogenicity	NN
cord-259738-yuqc6dk0	1	27	of	of	IN
cord-259738-yuqc6dk0	1	28	an	an	DT
cord-259738-yuqc6dk0	1	29	infectious	infectious	JJ
cord-259738-yuqc6dk0	1	30	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	1	31	virus	virus	NN
cord-259738-yuqc6dk0	1	32	DNA	dna	NN
cord-259738-yuqc6dk0	1	33	vaccine	vaccine	NN
cord-259738-yuqc6dk0	1	34	by	by	IN
cord-259738-yuqc6dk0	1	35	a	a	DT
cord-259738-yuqc6dk0	1	36	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	1	37	plasmid	plasmid	NN
cord-259738-yuqc6dk0	1	38	encoding	encoding	NN
cord-259738-yuqc6dk0	1	39	nucleocapsid	nucleocapsid	NNP
cord-259738-yuqc6dk0	1	40	protein	protein	NN
cord-259738-yuqc6dk0	1	41	and	and	CC
cord-259738-yuqc6dk0	1	42	interleukin-2	interleukin-2	IN
cord-259738-yuqc6dk0	1	43	date	date	NN
cord-259738-yuqc6dk0	1	44	:	:	:
cord-259738-yuqc6dk0	1	45	2008	2008	CD
cord-259738-yuqc6dk0	1	46	-	-	SYM
cord-259738-yuqc6dk0	1	47	03	03	CD
cord-259738-yuqc6dk0	1	48	-	-	HYPH
cord-259738-yuqc6dk0	1	49	07	07	CD
cord-259738-yuqc6dk0	1	50	journal	journal	NN
cord-259738-yuqc6dk0	1	51	:	:	:
cord-259738-yuqc6dk0	2	1	J	J	NNP
cord-259738-yuqc6dk0	2	2	Virol	Virol	NNP
cord-259738-yuqc6dk0	2	3	Methods	Methods	NNPS
cord-259738-yuqc6dk0	3	1	DOI	DOI	NNP
cord-259738-yuqc6dk0	3	2	:	:	:
cord-259738-yuqc6dk0	4	1	10.1016	10.1016	CD
cord-259738-yuqc6dk0	4	2	/	/	SYM
cord-259738-yuqc6dk0	4	3	j.jviromet.2008.01.017	j.jviromet.2008.01.017	.
cord-259738-yuqc6dk0	5	1	sha	sha	NNP
cord-259738-yuqc6dk0	5	2	:	:	:
cord-259738-yuqc6dk0	5	3	2feee102eda67b8b2839d523ecb483ad42b53bd7	2feee102eda67b8b2839d523ecb483ad42b53bd7	NNP
cord-259738-yuqc6dk0	5	4	doc_id	doc_id	CD
cord-259738-yuqc6dk0	5	5	:	:	:
cord-259738-yuqc6dk0	5	6	259738	259738	CD
cord-259738-yuqc6dk0	5	7	cord_uid	cord_uid	NNS
cord-259738-yuqc6dk0	5	8	:	:	:
cord-259738-yuqc6dk0	5	9	yuqc6dk0	yuqc6dk0	ADD
cord-259738-yuqc6dk0	6	1	A	a	DT
cord-259738-yuqc6dk0	6	2	DNA	dna	NN
cord-259738-yuqc6dk0	6	3	vaccine	vaccine	NN
cord-259738-yuqc6dk0	6	4	against	against	IN
cord-259738-yuqc6dk0	6	5	infectious	infectious	JJ
cord-259738-yuqc6dk0	6	6	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	6	7	virus	virus	NN
cord-259738-yuqc6dk0	6	8	(	(	-LRB-
cord-259738-yuqc6dk0	6	9	IBV	IBV	NNP
cord-259738-yuqc6dk0	6	10	)	)	-RRB-
cord-259738-yuqc6dk0	6	11	can	can	MD
cord-259738-yuqc6dk0	6	12	induce	induce	VB
cord-259738-yuqc6dk0	6	13	specific	specific	JJ
cord-259738-yuqc6dk0	6	14	humoral	humoral	JJ
cord-259738-yuqc6dk0	6	15	and	and	CC
cord-259738-yuqc6dk0	6	16	cell	cell	NN
cord-259738-yuqc6dk0	6	17	-	-	HYPH
cord-259738-yuqc6dk0	6	18	mediated	mediate	VBN
cord-259738-yuqc6dk0	6	19	immunity	immunity	NN
cord-259738-yuqc6dk0	6	20	.	.	.
cord-259738-yuqc6dk0	7	1	However	however	RB
cord-259738-yuqc6dk0	7	2	,	,	,
cord-259738-yuqc6dk0	7	3	compared	compare	VBN
cord-259738-yuqc6dk0	7	4	to	to	IN
cord-259738-yuqc6dk0	7	5	conventional	conventional	JJ
cord-259738-yuqc6dk0	7	6	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	7	7	,	,	,
cord-259738-yuqc6dk0	7	8	DNA	dna	NN
cord-259738-yuqc6dk0	7	9	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	7	10	usually	usually	RB
cord-259738-yuqc6dk0	7	11	induce	induce	VBP
cord-259738-yuqc6dk0	7	12	poor	poor	JJ
cord-259738-yuqc6dk0	7	13	antibody	antibody	NN
cord-259738-yuqc6dk0	7	14	responses	response	NNS
cord-259738-yuqc6dk0	7	15	.	.	.
cord-259738-yuqc6dk0	8	1	To	to	TO
cord-259738-yuqc6dk0	8	2	develop	develop	VB
cord-259738-yuqc6dk0	8	3	a	a	DT
cord-259738-yuqc6dk0	8	4	more	more	RBR
cord-259738-yuqc6dk0	8	5	potent	potent	JJ
cord-259738-yuqc6dk0	8	6	IBV	IBV	NNP
cord-259738-yuqc6dk0	8	7	DNA	dna	NN
cord-259738-yuqc6dk0	8	8	vaccine	vaccine	NN
cord-259738-yuqc6dk0	8	9	formulations	formulation	NNS
cord-259738-yuqc6dk0	8	10	,	,	,
cord-259738-yuqc6dk0	8	11	a	a	DT
cord-259738-yuqc6dk0	8	12	monocistronic	monocistronic	JJ
cord-259738-yuqc6dk0	8	13	vector	vector	NN
cord-259738-yuqc6dk0	8	14	encoding	encode	VBG
cord-259738-yuqc6dk0	8	15	the	the	DT
cord-259738-yuqc6dk0	8	16	nucleocapsid	nucleocapsid	NNP
cord-259738-yuqc6dk0	8	17	protein	protein	NN
cord-259738-yuqc6dk0	8	18	of	of	IN
cord-259738-yuqc6dk0	8	19	IBV	IBV	NNP
cord-259738-yuqc6dk0	8	20	and	and	CC
cord-259738-yuqc6dk0	8	21	a	a	DT
cord-259738-yuqc6dk0	8	22	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	8	23	vector	vector	NN
cord-259738-yuqc6dk0	8	24	separately	separately	RB
cord-259738-yuqc6dk0	8	25	encoding	encode	VBG
cord-259738-yuqc6dk0	8	26	the	the	DT
cord-259738-yuqc6dk0	8	27	nucleocapsid	nucleocapsid	NNP
cord-259738-yuqc6dk0	8	28	protein	protein	NN
cord-259738-yuqc6dk0	8	29	and	and	CC
cord-259738-yuqc6dk0	8	30	immune	immune	NN
cord-259738-yuqc6dk0	8	31	-	-	HYPH
cord-259738-yuqc6dk0	8	32	stimulatory	stimulatory	JJ
cord-259738-yuqc6dk0	8	33	interleukin-2	interleukin-2	NN
cord-259738-yuqc6dk0	8	34	were	be	VBD
cord-259738-yuqc6dk0	8	35	constructed	construct	VBN
cord-259738-yuqc6dk0	8	36	.	.	.
cord-259738-yuqc6dk0	9	1	When	when	WRB
cord-259738-yuqc6dk0	9	2	the	the	DT
cord-259738-yuqc6dk0	9	3	DNA	dna	NN
cord-259738-yuqc6dk0	9	4	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	9	5	were	be	VBD
cord-259738-yuqc6dk0	9	6	administered	administer	VBN
cord-259738-yuqc6dk0	9	7	to	to	IN
cord-259738-yuqc6dk0	9	8	the	the	DT
cord-259738-yuqc6dk0	9	9	quadriceps	quadriceps	JJ
cord-259738-yuqc6dk0	9	10	muscle	muscle	NN
cord-259738-yuqc6dk0	9	11	of	of	IN
cord-259738-yuqc6dk0	9	12	chickens	chicken	NNS
cord-259738-yuqc6dk0	9	13	,	,	,
cord-259738-yuqc6dk0	9	14	the	the	DT
cord-259738-yuqc6dk0	9	15	induced	induce	VBN
cord-259738-yuqc6dk0	9	16	humoral	humoral	JJ
cord-259738-yuqc6dk0	9	17	and	and	CC
cord-259738-yuqc6dk0	9	18	cellular	cellular	JJ
cord-259738-yuqc6dk0	9	19	responses	response	NNS
cord-259738-yuqc6dk0	9	20	were	be	VBD
cord-259738-yuqc6dk0	9	21	evaluated	evaluate	VBN
cord-259738-yuqc6dk0	9	22	.	.	.
cord-259738-yuqc6dk0	10	1	There	there	EX
cord-259738-yuqc6dk0	10	2	was	be	VBD
cord-259738-yuqc6dk0	10	3	a	a	DT
cord-259738-yuqc6dk0	10	4	significant	significant	JJ
cord-259738-yuqc6dk0	10	5	difference	difference	NN
cord-259738-yuqc6dk0	10	6	in	in	IN
cord-259738-yuqc6dk0	10	7	ELISA	ELISA	NNP
cord-259738-yuqc6dk0	10	8	antibody	antibody	NN
cord-259738-yuqc6dk0	10	9	levels	level	NNS
cord-259738-yuqc6dk0	10	10	elicited	elicit	VBN
cord-259738-yuqc6dk0	10	11	by	by	IN
cord-259738-yuqc6dk0	10	12	either	either	DT
cord-259738-yuqc6dk0	10	13	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	10	14	or	or	CC
cord-259738-yuqc6dk0	10	15	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	10	16	DNA	DNA	NNP
cord-259738-yuqc6dk0	10	17	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	10	18	.	.	.
cord-259738-yuqc6dk0	11	1	The	the	DT
cord-259738-yuqc6dk0	11	2	percentage	percentage	NN
cord-259738-yuqc6dk0	11	3	of	of	IN
cord-259738-yuqc6dk0	11	4	CD3(+	cd3(+	NN
cord-259738-yuqc6dk0	11	5	)	)	-RRB-
cord-259738-yuqc6dk0	11	6	,	,	,
cord-259738-yuqc6dk0	11	7	CD3(+)CD8(+	CD3(+)CD8(+	NNPS
cord-259738-yuqc6dk0	11	8	)	)	-RRB-
cord-259738-yuqc6dk0	11	9	and	and	CC
cord-259738-yuqc6dk0	11	10	CD3(+)CD4(+	CD3(+)CD4(+	NNP
cord-259738-yuqc6dk0	11	11	)	)	-RRB-
cord-259738-yuqc6dk0	11	12	subgroups	subgroup	NNS
cord-259738-yuqc6dk0	11	13	of	of	IN
cord-259738-yuqc6dk0	11	14	peripheral	peripheral	JJ
cord-259738-yuqc6dk0	11	15	blood	blood	NN
cord-259738-yuqc6dk0	11	16	T	t	NN
cord-259738-yuqc6dk0	11	17	-	-	HYPH
cord-259738-yuqc6dk0	11	18	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	11	19	in	in	IN
cord-259738-yuqc6dk0	11	20	chickens	chicken	NNS
cord-259738-yuqc6dk0	11	21	immunized	immunize	VBN
cord-259738-yuqc6dk0	11	22	with	with	IN
cord-259738-yuqc6dk0	11	23	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	11	24	DNA	DNA	NNP
cord-259738-yuqc6dk0	11	25	vaccine	vaccine	NN
cord-259738-yuqc6dk0	11	26	were	be	VBD
cord-259738-yuqc6dk0	11	27	higher	high	JJR
cord-259738-yuqc6dk0	11	28	than	than	IN
cord-259738-yuqc6dk0	11	29	those	those	DT
cord-259738-yuqc6dk0	11	30	in	in	IN
cord-259738-yuqc6dk0	11	31	chickens	chicken	NNS
cord-259738-yuqc6dk0	11	32	immunized	immunize	VBN
cord-259738-yuqc6dk0	11	33	with	with	IN
cord-259738-yuqc6dk0	11	34	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	11	35	DNA	DNA	NNP
cord-259738-yuqc6dk0	11	36	vaccine	vaccine	NN
cord-259738-yuqc6dk0	11	37	.	.	.
cord-259738-yuqc6dk0	12	1	When	when	WRB
cord-259738-yuqc6dk0	12	2	chickens	chicken	NNS
cord-259738-yuqc6dk0	12	3	were	be	VBD
cord-259738-yuqc6dk0	12	4	challenged	challenge	VBN
cord-259738-yuqc6dk0	12	5	with	with	IN
cord-259738-yuqc6dk0	12	6	a	a	DT
cord-259738-yuqc6dk0	12	7	virulent	virulent	JJ
cord-259738-yuqc6dk0	12	8	strain	strain	NN
cord-259738-yuqc6dk0	12	9	of	of	IN
cord-259738-yuqc6dk0	12	10	IBV	IBV	NNP
cord-259738-yuqc6dk0	12	11	,	,	,
cord-259738-yuqc6dk0	12	12	the	the	DT
cord-259738-yuqc6dk0	12	13	protective	protective	JJ
cord-259738-yuqc6dk0	12	14	efficacy	efficacy	NN
cord-259738-yuqc6dk0	12	15	could	could	MD
cord-259738-yuqc6dk0	12	16	be	be	VB
cord-259738-yuqc6dk0	12	17	enhanced	enhance	VBN
cord-259738-yuqc6dk0	12	18	significantly	significantly	RB
cord-259738-yuqc6dk0	12	19	after	after	IN
cord-259738-yuqc6dk0	12	20	immunization	immunization	NN
cord-259738-yuqc6dk0	12	21	with	with	IN
cord-259738-yuqc6dk0	12	22	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	12	23	DNA	DNA	NNP
cord-259738-yuqc6dk0	12	24	vaccine	vaccine	NN
cord-259738-yuqc6dk0	12	25	.	.	.
cord-259738-yuqc6dk0	13	1	These	these	DT
cord-259738-yuqc6dk0	13	2	results	result	NNS
cord-259738-yuqc6dk0	13	3	demonstrated	demonstrate	VBD
cord-259738-yuqc6dk0	13	4	that	that	IN
cord-259738-yuqc6dk0	13	5	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	13	6	DNA	dna	NN
cord-259738-yuqc6dk0	13	7	vaccine	vaccine	NN
cord-259738-yuqc6dk0	13	8	is	be	VBZ
cord-259738-yuqc6dk0	13	9	an	an	DT
cord-259738-yuqc6dk0	13	10	effective	effective	JJ
cord-259738-yuqc6dk0	13	11	approach	approach	NN
cord-259738-yuqc6dk0	13	12	to	to	TO
cord-259738-yuqc6dk0	13	13	increase	increase	VB
cord-259738-yuqc6dk0	13	14	IBV	IBV	NNP
cord-259738-yuqc6dk0	13	15	DNA	DNA	NNP
cord-259738-yuqc6dk0	13	16	vaccine	vaccine	NN
cord-259738-yuqc6dk0	13	17	immunogenicity	immunogenicity	NN
cord-259738-yuqc6dk0	13	18	.	.	.
cord-259738-yuqc6dk0	14	1	Infectious	infectious	JJ
cord-259738-yuqc6dk0	14	2	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	14	3	(	(	-LRB-
cord-259738-yuqc6dk0	14	4	IB	IB	NNP
cord-259738-yuqc6dk0	14	5	)	)	-RRB-
cord-259738-yuqc6dk0	14	6	is	be	VBZ
cord-259738-yuqc6dk0	14	7	an	an	DT
cord-259738-yuqc6dk0	14	8	acute	acute	JJ
cord-259738-yuqc6dk0	14	9	and	and	CC
cord-259738-yuqc6dk0	14	10	highly	highly	RB
cord-259738-yuqc6dk0	14	11	contagious	contagious	JJ
cord-259738-yuqc6dk0	14	12	respiratory	respiratory	JJ
cord-259738-yuqc6dk0	14	13	disease	disease	NN
cord-259738-yuqc6dk0	14	14	of	of	IN
cord-259738-yuqc6dk0	14	15	chickens	chicken	NNS
cord-259738-yuqc6dk0	14	16	.	.	.
cord-259738-yuqc6dk0	15	1	It	-PRON-	PRP
cord-259738-yuqc6dk0	15	2	is	be	VBZ
cord-259738-yuqc6dk0	15	3	still	still	RB
cord-259738-yuqc6dk0	15	4	a	a	DT
cord-259738-yuqc6dk0	15	5	major	major	JJ
cord-259738-yuqc6dk0	15	6	health	health	NN
cord-259738-yuqc6dk0	15	7	problem	problem	NN
cord-259738-yuqc6dk0	15	8	in	in	IN
cord-259738-yuqc6dk0	15	9	the	the	DT
cord-259738-yuqc6dk0	15	10	chicken	chicken	NN
cord-259738-yuqc6dk0	15	11	industry	industry	NN
cord-259738-yuqc6dk0	15	12	in	in	IN
cord-259738-yuqc6dk0	15	13	the	the	DT
cord-259738-yuqc6dk0	15	14	world	world	NN
cord-259738-yuqc6dk0	15	15	.	.	.
cord-259738-yuqc6dk0	16	1	Vaccination	vaccination	NN
cord-259738-yuqc6dk0	16	2	to	to	TO
cord-259738-yuqc6dk0	16	3	control	control	VB
cord-259738-yuqc6dk0	16	4	IB	IB	NNP
cord-259738-yuqc6dk0	16	5	has	have	VBZ
cord-259738-yuqc6dk0	16	6	been	be	VBN
cord-259738-yuqc6dk0	16	7	practiced	practice	VBN
cord-259738-yuqc6dk0	16	8	for	for	IN
cord-259738-yuqc6dk0	16	9	over	over	IN
cord-259738-yuqc6dk0	16	10	half	half	PDT
cord-259738-yuqc6dk0	16	11	a	a	DT
cord-259738-yuqc6dk0	16	12	century	century	NN
cord-259738-yuqc6dk0	16	13	(	(	-LRB-
cord-259738-yuqc6dk0	16	14	Bijlenga	Bijlenga	NNP
cord-259738-yuqc6dk0	16	15	et	et	NNP
cord-259738-yuqc6dk0	16	16	al	al	NNP
cord-259738-yuqc6dk0	16	17	.	.	NNP
cord-259738-yuqc6dk0	16	18	,	,	,
cord-259738-yuqc6dk0	16	19	2004	2004	CD
cord-259738-yuqc6dk0	16	20	;	;	:
cord-259738-yuqc6dk0	16	21	Cavanagh	Cavanagh	NNP
cord-259738-yuqc6dk0	16	22	and	and	CC
cord-259738-yuqc6dk0	16	23	Naqi	Naqi	NNP
cord-259738-yuqc6dk0	16	24	,	,	,
cord-259738-yuqc6dk0	16	25	2003	2003	CD
cord-259738-yuqc6dk0	16	26	;	;	:
cord-259738-yuqc6dk0	16	27	Cavanagh	Cavanagh	NNP
cord-259738-yuqc6dk0	16	28	,	,	,
cord-259738-yuqc6dk0	16	29	2003	2003	CD
cord-259738-yuqc6dk0	16	30	)	)	-RRB-
cord-259738-yuqc6dk0	16	31	.	.	.
cord-259738-yuqc6dk0	17	1	Such	such	JJ
cord-259738-yuqc6dk0	17	2	conventional	conventional	JJ
cord-259738-yuqc6dk0	17	3	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	17	4	,	,	,
cord-259738-yuqc6dk0	17	5	although	although	IN
cord-259738-yuqc6dk0	17	6	generally	generally	RB
cord-259738-yuqc6dk0	17	7	effective	effective	JJ
cord-259738-yuqc6dk0	17	8	,	,	,
cord-259738-yuqc6dk0	17	9	do	do	VBP
cord-259738-yuqc6dk0	17	10	have	have	VB
cord-259738-yuqc6dk0	17	11	some	some	DT
cord-259738-yuqc6dk0	17	12	disadvantages	disadvantage	NNS
cord-259738-yuqc6dk0	17	13	.	.	.
cord-259738-yuqc6dk0	18	1	Attenuated	attenuated	JJ
cord-259738-yuqc6dk0	18	2	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	18	3	,	,	,
cord-259738-yuqc6dk0	18	4	which	which	WDT
cord-259738-yuqc6dk0	18	5	generally	generally	RB
cord-259738-yuqc6dk0	18	6	induce	induce	VBP
cord-259738-yuqc6dk0	18	7	long	long	RB
cord-259738-yuqc6dk0	18	8	-	-	HYPH
cord-259738-yuqc6dk0	18	9	lasting	last	VBG
cord-259738-yuqc6dk0	18	10	immunity	immunity	NN
cord-259738-yuqc6dk0	18	11	,	,	,
cord-259738-yuqc6dk0	18	12	have	have	VB
cord-259738-yuqc6dk0	18	13	a	a	DT
cord-259738-yuqc6dk0	18	14	risk	risk	NN
cord-259738-yuqc6dk0	18	15	of	of	IN
cord-259738-yuqc6dk0	18	16	insufficient	insufficient	JJ
cord-259738-yuqc6dk0	18	17	attenuation	attenuation	NN
cord-259738-yuqc6dk0	18	18	and/or	and/or	CC
cord-259738-yuqc6dk0	18	19	genetic	genetic	JJ
cord-259738-yuqc6dk0	18	20	instability	instability	NN
cord-259738-yuqc6dk0	19	1	(	(	-LRB-
cord-259738-yuqc6dk0	19	2	Cook	Cook	NNP
cord-259738-yuqc6dk0	19	3	et	et	NNP
cord-259738-yuqc6dk0	19	4	al	al	NNP
cord-259738-yuqc6dk0	19	5	.	.	NNP
cord-259738-yuqc6dk0	19	6	,	,	,
cord-259738-yuqc6dk0	19	7	1986	1986	CD
cord-259738-yuqc6dk0	19	8	)	)	-RRB-
cord-259738-yuqc6dk0	19	9	.	.	.
cord-259738-yuqc6dk0	20	1	The	the	DT
cord-259738-yuqc6dk0	20	2	limitations	limitation	NNS
cord-259738-yuqc6dk0	20	3	of	of	IN
cord-259738-yuqc6dk0	20	4	inactivated	inactivate	VBN
cord-259738-yuqc6dk0	20	5	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	20	6	include	include	VBP
cord-259738-yuqc6dk0	20	7	high	high	JJ
cord-259738-yuqc6dk0	20	8	cost	cost	NN
cord-259738-yuqc6dk0	20	9	for	for	IN
cord-259738-yuqc6dk0	20	10	manufacturing	manufacturing	NN
cord-259738-yuqc6dk0	20	11	and	and	CC
cord-259738-yuqc6dk0	20	12	lack	lack	NN
cord-259738-yuqc6dk0	20	13	of	of	IN
cord-259738-yuqc6dk0	20	14	long	long	JJ
cord-259738-yuqc6dk0	20	15	-	-	HYPH
cord-259738-yuqc6dk0	20	16	term	term	NN
cord-259738-yuqc6dk0	20	17	immunity	immunity	NN
cord-259738-yuqc6dk0	20	18	.	.	.
cord-259738-yuqc6dk0	21	1	Therefore	therefore	RB
cord-259738-yuqc6dk0	21	2	a	a	DT
cord-259738-yuqc6dk0	21	3	new	new	JJ
cord-259738-yuqc6dk0	21	4	generation	generation	NN
cord-259738-yuqc6dk0	21	5	of	of	IN
cord-259738-yuqc6dk0	21	6	IB	IB	NNP
cord-259738-yuqc6dk0	21	7	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	21	8	is	be	VBZ
cord-259738-yuqc6dk0	21	9	called	call	VBN
cord-259738-yuqc6dk0	21	10	for	for	IN
cord-259738-yuqc6dk0	21	11	.	.	.
cord-259738-yuqc6dk0	22	1	Infectious	infectious	JJ
cord-259738-yuqc6dk0	22	2	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	22	3	virus	virus	NN
cord-259738-yuqc6dk0	22	4	(	(	-LRB-
cord-259738-yuqc6dk0	22	5	IBV	IBV	NNP
cord-259738-yuqc6dk0	22	6	)	)	-RRB-
cord-259738-yuqc6dk0	22	7	is	be	VBZ
cord-259738-yuqc6dk0	22	8	an	an	DT
cord-259738-yuqc6dk0	22	9	enveloped	envelop	VBN
cord-259738-yuqc6dk0	22	10	coronavirus	coronavirus	NN
cord-259738-yuqc6dk0	22	11	that	that	WDT
cord-259738-yuqc6dk0	22	12	contains	contain	VBZ
cord-259738-yuqc6dk0	22	13	an	an	DT
cord-259738-yuqc6dk0	22	14	unsegmented	unsegmented	JJ
cord-259738-yuqc6dk0	22	15	,	,	,
cord-259738-yuqc6dk0	22	16	single	single	RB
cord-259738-yuqc6dk0	22	17	-	-	HYPH
cord-259738-yuqc6dk0	22	18	stranded	stranded	JJ
cord-259738-yuqc6dk0	22	19	,	,	,
cord-259738-yuqc6dk0	22	20	positive	positive	JJ
cord-259738-yuqc6dk0	22	21	-	-	HYPH
cord-259738-yuqc6dk0	22	22	sense	sense	NN
cord-259738-yuqc6dk0	22	23	RNA	RNA	NNP
cord-259738-yuqc6dk0	22	24	genome	genome	NN
cord-259738-yuqc6dk0	22	25	.	.	.
cord-259738-yuqc6dk0	23	1	In	in	IN
cord-259738-yuqc6dk0	23	2	addition	addition	NN
cord-259738-yuqc6dk0	23	3	to	to	IN
cord-259738-yuqc6dk0	23	4	the	the	DT
cord-259738-yuqc6dk0	23	5	internally	internally	RB
cord-259738-yuqc6dk0	23	6	localized	localize	VBN
cord-259738-yuqc6dk0	23	7	nucleocapsid	nucleocapsid	NN
cord-259738-yuqc6dk0	23	8	(	(	-LRB-
cord-259738-yuqc6dk0	23	9	N	N	NNP
cord-259738-yuqc6dk0	23	10	)	)	-RRB-
cord-259738-yuqc6dk0	23	11	protein	protein	NN
cord-259738-yuqc6dk0	23	12	,	,	,
cord-259738-yuqc6dk0	23	13	the	the	DT
cord-259738-yuqc6dk0	23	14	virion	virion	NN
cord-259738-yuqc6dk0	23	15	is	be	VBZ
cord-259738-yuqc6dk0	23	16	composed	compose	VBN
cord-259738-yuqc6dk0	23	17	of	of	IN
cord-259738-yuqc6dk0	23	18	spike	spike	NN
cord-259738-yuqc6dk0	23	19	protein	protein	NN
cord-259738-yuqc6dk0	23	20	,	,	,
cord-259738-yuqc6dk0	23	21	membrane	membrane	NN
cord-259738-yuqc6dk0	23	22	protein	protein	NN
cord-259738-yuqc6dk0	23	23	and	and	CC
cord-259738-yuqc6dk0	23	24	a	a	DT
cord-259738-yuqc6dk0	23	25	small	small	JJ
cord-259738-yuqc6dk0	23	26	envelope	envelope	NN
cord-259738-yuqc6dk0	23	27	protein	protein	NN
cord-259738-yuqc6dk0	23	28	.	.	.
cord-259738-yuqc6dk0	24	1	N	N	NNP
cord-259738-yuqc6dk0	24	2	protein	protein	NN
cord-259738-yuqc6dk0	24	3	carries	carry	VBZ
cord-259738-yuqc6dk0	24	4	epitopes	epitope	NNS
cord-259738-yuqc6dk0	24	5	inducing	induce	VBG
cord-259738-yuqc6dk0	24	6	cross	cross	JJ
cord-259738-yuqc6dk0	24	7	-	-	JJ
cord-259738-yuqc6dk0	24	8	reactive	reactive	JJ
cord-259738-yuqc6dk0	24	9	antibodies	antibody	NNS
cord-259738-yuqc6dk0	24	10	and	and	CC
cord-259738-yuqc6dk0	24	11	is	be	VBZ
cord-259738-yuqc6dk0	24	12	the	the	DT
cord-259738-yuqc6dk0	24	13	most	most	RBS
cord-259738-yuqc6dk0	24	14	abundant	abundant	JJ
cord-259738-yuqc6dk0	24	15	virus	virus	NN
cord-259738-yuqc6dk0	24	16	-	-	HYPH
cord-259738-yuqc6dk0	24	17	derived	derive	VBN
cord-259738-yuqc6dk0	24	18	protein	protein	NN
cord-259738-yuqc6dk0	24	19	produced	produce	VBN
cord-259738-yuqc6dk0	24	20	throughout	throughout	IN
cord-259738-yuqc6dk0	24	21	infection	infection	NN
cord-259738-yuqc6dk0	24	22	(	(	-LRB-
cord-259738-yuqc6dk0	24	23	Seah	Seah	NNP
cord-259738-yuqc6dk0	24	24	et	et	FW
cord-259738-yuqc6dk0	24	25	al	al	NNP
cord-259738-yuqc6dk0	24	26	.	.	NNP
cord-259738-yuqc6dk0	24	27	,	,	,
cord-259738-yuqc6dk0	24	28	2000	2000	CD
cord-259738-yuqc6dk0	24	29	;	;	:
cord-259738-yuqc6dk0	24	30	Boots	Boots	NNP
cord-259738-yuqc6dk0	24	31	et	et	NNP
cord-259738-yuqc6dk0	24	32	al	al	NNP
cord-259738-yuqc6dk0	24	33	.	.	NNP
cord-259738-yuqc6dk0	24	34	,	,	,
cord-259738-yuqc6dk0	24	35	1992	1992	CD
cord-259738-yuqc6dk0	24	36	;	;	:
cord-259738-yuqc6dk0	24	37	Yu	Yu	NNP
cord-259738-yuqc6dk0	24	38	et	et	NNP
cord-259738-yuqc6dk0	24	39	al	al	NNP
cord-259738-yuqc6dk0	24	40	.	.	NNP
cord-259738-yuqc6dk0	24	41	,	,	,
cord-259738-yuqc6dk0	24	42	2001	2001	CD
cord-259738-yuqc6dk0	24	43	)	)	-RRB-
cord-259738-yuqc6dk0	24	44	.	.	.
cord-259738-yuqc6dk0	25	1	The	the	DT
cord-259738-yuqc6dk0	25	2	carboxyl	carboxyl	NN
cord-259738-yuqc6dk0	25	3	end	end	NN
cord-259738-yuqc6dk0	25	4	of	of	IN
cord-259738-yuqc6dk0	25	5	N	n	CD
cord-259738-yuqc6dk0	25	6	protein	protein	NN
cord-259738-yuqc6dk0	25	7	has	have	VBZ
cord-259738-yuqc6dk0	25	8	CTL	CTL	NNP
cord-259738-yuqc6dk0	25	9	epitopes	epitope	NNS
cord-259738-yuqc6dk0	25	10	for	for	IN
cord-259738-yuqc6dk0	25	11	MHC	MHC	NNP
cord-259738-yuqc6dk0	25	12	compatible	compatible	JJ
cord-259738-yuqc6dk0	25	13	chickens	chicken	NNS
cord-259738-yuqc6dk0	25	14	.	.	.
cord-259738-yuqc6dk0	26	1	Chickens	chicken	NNS
cord-259738-yuqc6dk0	26	2	inoculated	inoculate	VBN
cord-259738-yuqc6dk0	26	3	with	with	IN
cord-259738-yuqc6dk0	26	4	DNA	dna	NN
cord-259738-yuqc6dk0	26	5	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	26	6	expressing	express	VBG
cord-259738-yuqc6dk0	26	7	the	the	DT
cord-259738-yuqc6dk0	26	8	carboxyl	carboxyl	NN
cord-259738-yuqc6dk0	26	9	end	end	NN
cord-259738-yuqc6dk0	26	10	of	of	IN
cord-259738-yuqc6dk0	26	11	N	n	CD
cord-259738-yuqc6dk0	26	12	were	be	VBD
cord-259738-yuqc6dk0	26	13	protected	protect	VBN
cord-259738-yuqc6dk0	26	14	from	from	IN
cord-259738-yuqc6dk0	26	15	IBV	IBV	NNP
cord-259738-yuqc6dk0	26	16	infection	infection	NN
cord-259738-yuqc6dk0	26	17	.	.	.
cord-259738-yuqc6dk0	27	1	In	in	IN
cord-259738-yuqc6dk0	27	2	recent	recent	JJ
cord-259738-yuqc6dk0	27	3	years	year	NNS
cord-259738-yuqc6dk0	27	4	,	,	,
cord-259738-yuqc6dk0	27	5	the	the	DT
cord-259738-yuqc6dk0	27	6	development	development	NN
cord-259738-yuqc6dk0	27	7	of	of	IN
cord-259738-yuqc6dk0	27	8	DNA	dna	NN
cord-259738-yuqc6dk0	27	9	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	27	10	as	as	IN
cord-259738-yuqc6dk0	27	11	a	a	DT
cord-259738-yuqc6dk0	27	12	potentially	potentially	RB
cord-259738-yuqc6dk0	27	13	safe	safe	JJ
cord-259738-yuqc6dk0	27	14	alternative	alternative	NN
cord-259738-yuqc6dk0	27	15	has	have	VBZ
cord-259738-yuqc6dk0	27	16	been	be	VBN
cord-259738-yuqc6dk0	27	17	explored	explore	VBN
cord-259738-yuqc6dk0	27	18	.	.	.
cord-259738-yuqc6dk0	28	1	DNA	dna	NN
cord-259738-yuqc6dk0	28	2	immunization	immunization	NN
cord-259738-yuqc6dk0	28	3	is	be	VBZ
cord-259738-yuqc6dk0	28	4	an	an	DT
cord-259738-yuqc6dk0	28	5	important	important	JJ
cord-259738-yuqc6dk0	28	6	vaccination	vaccination	NN
cord-259738-yuqc6dk0	28	7	strategy	strategy	NN
cord-259738-yuqc6dk0	28	8	that	that	WDT
cord-259738-yuqc6dk0	28	9	has	have	VBZ
cord-259738-yuqc6dk0	28	10	many	many	JJ
cord-259738-yuqc6dk0	28	11	characters	character	NNS
cord-259738-yuqc6dk0	28	12	desirable	desirable	JJ
cord-259738-yuqc6dk0	28	13	for	for	IN
cord-259738-yuqc6dk0	28	14	an	an	DT
cord-259738-yuqc6dk0	28	15	ideal	ideal	JJ
cord-259738-yuqc6dk0	28	16	vaccine	vaccine	NN
cord-259738-yuqc6dk0	28	17	,	,	,
cord-259738-yuqc6dk0	28	18	including	include	VBG
cord-259738-yuqc6dk0	28	19	induction	induction	NN
cord-259738-yuqc6dk0	28	20	of	of	IN
cord-259738-yuqc6dk0	28	21	broad	broad	JJ
cord-259738-yuqc6dk0	28	22	immune	immune	JJ
cord-259738-yuqc6dk0	28	23	responses	response	NNS
cord-259738-yuqc6dk0	28	24	,	,	,
cord-259738-yuqc6dk0	28	25	long	long	RB
cord-259738-yuqc6dk0	28	26	-	-	HYPH
cord-259738-yuqc6dk0	28	27	lasting	last	VBG
cord-259738-yuqc6dk0	28	28	immunity	immunity	NN
cord-259738-yuqc6dk0	28	29	and	and	CC
cord-259738-yuqc6dk0	28	30	simple	simple	JJ
cord-259738-yuqc6dk0	28	31	and	and	CC
cord-259738-yuqc6dk0	28	32	cheap	cheap	JJ
cord-259738-yuqc6dk0	28	33	production	production	NN
cord-259738-yuqc6dk0	28	34	.	.	.
cord-259738-yuqc6dk0	29	1	Experimental	experimental	JJ
cord-259738-yuqc6dk0	29	2	DNA	dna	NN
cord-259738-yuqc6dk0	29	3	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	29	4	against	against	IN
cord-259738-yuqc6dk0	29	5	viral	viral	JJ
cord-259738-yuqc6dk0	29	6	,	,	,
cord-259738-yuqc6dk0	29	7	bacterial	bacterial	JJ
cord-259738-yuqc6dk0	29	8	,	,	,
cord-259738-yuqc6dk0	29	9	and	and	CC
cord-259738-yuqc6dk0	29	10	parasitic	parasitic	JJ
cord-259738-yuqc6dk0	29	11	disease	disease	NN
cord-259738-yuqc6dk0	29	12	have	have	VBP
cord-259738-yuqc6dk0	29	13	been	be	VBN
cord-259738-yuqc6dk0	29	14	described	describe	VBN
cord-259738-yuqc6dk0	29	15	(	(	-LRB-
cord-259738-yuqc6dk0	30	1	Tacket	Tacket	NNP
cord-259738-yuqc6dk0	30	2	et	et	NNP
cord-259738-yuqc6dk0	30	3	al	al	NNP
cord-259738-yuqc6dk0	30	4	.	.	.
cord-259738-yuqc6dk0	31	1	,	,	,
cord-259738-yuqc6dk0	31	2	1999	1999	CD
cord-259738-yuqc6dk0	31	3	;	;	:
cord-259738-yuqc6dk0	31	4	Strugnell	Strugnell	NNP
cord-259738-yuqc6dk0	31	5	et	et	FW
cord-259738-yuqc6dk0	31	6	al	al	NNP
cord-259738-yuqc6dk0	31	7	.	.	NNP
cord-259738-yuqc6dk0	31	8	,	,	,
cord-259738-yuqc6dk0	31	9	1997	1997	CD
cord-259738-yuqc6dk0	31	10	;	;	:
cord-259738-yuqc6dk0	31	11	Kalinna	Kalinna	NNP
cord-259738-yuqc6dk0	31	12	,	,	,
cord-259738-yuqc6dk0	31	13	1997	1997	CD
cord-259738-yuqc6dk0	31	14	)	)	-RRB-
cord-259738-yuqc6dk0	31	15	and	and	CC
cord-259738-yuqc6dk0	31	16	DNA	dna	NN
cord-259738-yuqc6dk0	31	17	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	31	18	have	have	VBP
cord-259738-yuqc6dk0	31	19	been	be	VBN
cord-259738-yuqc6dk0	31	20	licensed	license	VBN
cord-259738-yuqc6dk0	31	21	for	for	IN
cord-259738-yuqc6dk0	31	22	two	two	CD
cord-259738-yuqc6dk0	31	23	nonhuman	nonhuman	NN
cord-259738-yuqc6dk0	31	24	applications	application	NNS
cord-259738-yuqc6dk0	31	25	:	:	:
cord-259738-yuqc6dk0	31	26	one	one	CD
cord-259738-yuqc6dk0	31	27	for	for	IN
cord-259738-yuqc6dk0	31	28	West	West	NNP
cord-259738-yuqc6dk0	31	29	Nile	Nile	NNP
cord-259738-yuqc6dk0	31	30	virus	virus	NN
cord-259738-yuqc6dk0	31	31	for	for	IN
cord-259738-yuqc6dk0	31	32	horses	horse	NNS
cord-259738-yuqc6dk0	31	33	(	(	-LRB-
cord-259738-yuqc6dk0	31	34	Powell	Powell	NNP
cord-259738-yuqc6dk0	31	35	,	,	,
cord-259738-yuqc6dk0	31	36	2004	2004	CD
cord-259738-yuqc6dk0	31	37	)	)	-RRB-
cord-259738-yuqc6dk0	31	38	and	and	CC
cord-259738-yuqc6dk0	31	39	the	the	DT
cord-259738-yuqc6dk0	31	40	other	other	JJ
cord-259738-yuqc6dk0	31	41	for	for	IN
cord-259738-yuqc6dk0	31	42	infectious	infectious	JJ
cord-259738-yuqc6dk0	31	43	hematopoietic	hematopoietic	JJ
cord-259738-yuqc6dk0	31	44	necrosis	necrosis	NN
cord-259738-yuqc6dk0	31	45	virus	virus	NN
cord-259738-yuqc6dk0	31	46	for	for	IN
cord-259738-yuqc6dk0	31	47	salmon	salmon	NN
cord-259738-yuqc6dk0	31	48	(	(	-LRB-
cord-259738-yuqc6dk0	31	49	Lorenzen	Lorenzen	NNP
cord-259738-yuqc6dk0	31	50	and	and	CC
cord-259738-yuqc6dk0	31	51	Lapatra	Lapatra	NNP
cord-259738-yuqc6dk0	31	52	,	,	,
cord-259738-yuqc6dk0	31	53	2005	2005	CD
cord-259738-yuqc6dk0	31	54	)	)	-RRB-
cord-259738-yuqc6dk0	31	55	.	.	.
cord-259738-yuqc6dk0	32	1	Hence	hence	RB
cord-259738-yuqc6dk0	32	2	DNA	dna	NN
cord-259738-yuqc6dk0	32	3	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	32	4	appear	appear	VBP
cord-259738-yuqc6dk0	32	5	to	to	TO
cord-259738-yuqc6dk0	32	6	be	be	VB
cord-259738-yuqc6dk0	32	7	a	a	DT
cord-259738-yuqc6dk0	32	8	useful	useful	JJ
cord-259738-yuqc6dk0	32	9	technology	technology	NN
cord-259738-yuqc6dk0	32	10	.	.	.
cord-259738-yuqc6dk0	33	1	It	-PRON-	PRP
cord-259738-yuqc6dk0	33	2	is	be	VBZ
cord-259738-yuqc6dk0	33	3	believed	believe	VBN
cord-259738-yuqc6dk0	33	4	that	that	IN
cord-259738-yuqc6dk0	33	5	delivery	delivery	NN
cord-259738-yuqc6dk0	33	6	of	of	IN
cord-259738-yuqc6dk0	33	7	only	only	RB
cord-259738-yuqc6dk0	33	8	a	a	DT
cord-259738-yuqc6dk0	33	9	single	single	JJ
cord-259738-yuqc6dk0	33	10	DNA	dna	NN
cord-259738-yuqc6dk0	33	11	plasmid	plasmid	NN
cord-259738-yuqc6dk0	33	12	of	of	IN
cord-259738-yuqc6dk0	33	13	antigen	antigen	NN
cord-259738-yuqc6dk0	33	14	is	be	VBZ
cord-259738-yuqc6dk0	33	15	not	not	RB
cord-259738-yuqc6dk0	33	16	optimal	optimal	JJ
cord-259738-yuqc6dk0	33	17	to	to	TO
cord-259738-yuqc6dk0	33	18	protect	protect	VB
cord-259738-yuqc6dk0	33	19	against	against	IN
cord-259738-yuqc6dk0	33	20	infection	infection	NN
cord-259738-yuqc6dk0	33	21	.	.	.
cord-259738-yuqc6dk0	34	1	Adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	34	2	are	be	VBP
cord-259738-yuqc6dk0	34	3	used	use	VBN
cord-259738-yuqc6dk0	34	4	widely	widely	RB
cord-259738-yuqc6dk0	34	5	in	in	IN
cord-259738-yuqc6dk0	34	6	various	various	JJ
cord-259738-yuqc6dk0	34	7	vaccine	vaccine	NN
cord-259738-yuqc6dk0	34	8	formulations	formulation	NNS
cord-259738-yuqc6dk0	34	9	for	for	IN
cord-259738-yuqc6dk0	34	10	the	the	DT
cord-259738-yuqc6dk0	34	11	enhancement	enhancement	NN
cord-259738-yuqc6dk0	34	12	of	of	IN
cord-259738-yuqc6dk0	34	13	immune	immune	JJ
cord-259738-yuqc6dk0	34	14	responses	response	NNS
cord-259738-yuqc6dk0	34	15	.	.	.
cord-259738-yuqc6dk0	35	1	Among	among	IN
cord-259738-yuqc6dk0	35	2	the	the	DT
cord-259738-yuqc6dk0	35	3	adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	35	4	,	,	,
cord-259738-yuqc6dk0	35	5	cytokines	cytokine	NNS
cord-259738-yuqc6dk0	35	6	have	have	VBP
cord-259738-yuqc6dk0	35	7	been	be	VBN
cord-259738-yuqc6dk0	35	8	explored	explore	VBN
cord-259738-yuqc6dk0	35	9	extensively	extensively	RB
cord-259738-yuqc6dk0	35	10	to	to	TO
cord-259738-yuqc6dk0	35	11	augment	augment	VB
cord-259738-yuqc6dk0	35	12	the	the	DT
cord-259738-yuqc6dk0	35	13	potency	potency	NN
cord-259738-yuqc6dk0	35	14	of	of	IN
cord-259738-yuqc6dk0	35	15	DNA	dna	NN
cord-259738-yuqc6dk0	35	16	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	35	17	(	(	-LRB-
cord-259738-yuqc6dk0	35	18	Gurunathan	Gurunathan	NNP
cord-259738-yuqc6dk0	35	19	et	et	NNP
cord-259738-yuqc6dk0	35	20	al	al	NNP
cord-259738-yuqc6dk0	35	21	.	.	NNP
cord-259738-yuqc6dk0	35	22	,	,	,
cord-259738-yuqc6dk0	35	23	2000	2000	CD
cord-259738-yuqc6dk0	35	24	;	;	:
cord-259738-yuqc6dk0	35	25	Reyes	Reyes	NNP
cord-259738-yuqc6dk0	35	26	-	-	HYPH
cord-259738-yuqc6dk0	35	27	Sandoval	Sandoval	NNP
cord-259738-yuqc6dk0	35	28	and	and	CC
cord-259738-yuqc6dk0	35	29	Ertl	Ertl	NNP
cord-259738-yuqc6dk0	35	30	,	,	,
cord-259738-yuqc6dk0	35	31	2001	2001	CD
cord-259738-yuqc6dk0	35	32	;	;	:
cord-259738-yuqc6dk0	35	33	Scheerlinck	Scheerlinck	NNP
cord-259738-yuqc6dk0	35	34	,	,	,
cord-259738-yuqc6dk0	35	35	2001	2001	CD
cord-259738-yuqc6dk0	35	36	;	;	:
cord-259738-yuqc6dk0	35	37	Barouch	Barouch	NNP
cord-259738-yuqc6dk0	35	38	et	et	FW
cord-259738-yuqc6dk0	35	39	al	al	NNP
cord-259738-yuqc6dk0	35	40	.	.	NNP
cord-259738-yuqc6dk0	35	41	,	,	,
cord-259738-yuqc6dk0	35	42	2004	2004	CD
cord-259738-yuqc6dk0	35	43	;	;	:
cord-259738-yuqc6dk0	35	44	Calarota	Calarota	NNP
cord-259738-yuqc6dk0	35	45	and	and	CC
cord-259738-yuqc6dk0	35	46	Weiner	Weiner	NNP
cord-259738-yuqc6dk0	35	47	,	,	,
cord-259738-yuqc6dk0	35	48	2004	2004	CD
cord-259738-yuqc6dk0	35	49	;	;	:
cord-259738-yuqc6dk0	35	50	Stevenson	Stevenson	NNP
cord-259738-yuqc6dk0	35	51	,	,	,
cord-259738-yuqc6dk0	35	52	2004	2004	CD
cord-259738-yuqc6dk0	35	53	;	;	:
cord-259738-yuqc6dk0	35	54	Barouch	Barouch	NNP
cord-259738-yuqc6dk0	35	55	,	,	,
cord-259738-yuqc6dk0	35	56	2006	2006	CD
cord-259738-yuqc6dk0	35	57	)	)	-RRB-
cord-259738-yuqc6dk0	35	58	.	.	.
cord-259738-yuqc6dk0	36	1	Interleukin-2	interleukin-2	XX
cord-259738-yuqc6dk0	37	1	(	(	-LRB-
cord-259738-yuqc6dk0	37	2	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	37	3	)	)	-RRB-
cord-259738-yuqc6dk0	37	4	,	,	,
cord-259738-yuqc6dk0	37	5	initially	initially	RB
cord-259738-yuqc6dk0	37	6	known	know	VBN
cord-259738-yuqc6dk0	37	7	as	as	IN
cord-259738-yuqc6dk0	37	8	T	t	NN
cord-259738-yuqc6dk0	37	9	-	-	HYPH
cord-259738-yuqc6dk0	37	10	cell	cell	NN
cord-259738-yuqc6dk0	37	11	growth	growth	NN
cord-259738-yuqc6dk0	37	12	factor	factor	NN
cord-259738-yuqc6dk0	37	13	,	,	,
cord-259738-yuqc6dk0	37	14	is	be	VBZ
cord-259738-yuqc6dk0	37	15	a	a	DT
cord-259738-yuqc6dk0	37	16	powerful	powerful	JJ
cord-259738-yuqc6dk0	37	17	immunoregulatory	immunoregulatory	JJ
cord-259738-yuqc6dk0	37	18	lymphokine	lymphokine	NN
cord-259738-yuqc6dk0	37	19	which	which	WDT
cord-259738-yuqc6dk0	37	20	produced	produce	VBN
cord-259738-yuqc6dk0	37	21	by	by	IN
cord-259738-yuqc6dk0	37	22	lectin	lectin	NN
cord-259738-yuqc6dk0	37	23	-	-	HYPH
cord-259738-yuqc6dk0	37	24	or	or	CC
cord-259738-yuqc6dk0	37	25	antigen	antigen	NN
cord-259738-yuqc6dk0	37	26	-	-	HYPH
cord-259738-yuqc6dk0	37	27	activated	activate	VBN
cord-259738-yuqc6dk0	37	28	T	t	NN
cord-259738-yuqc6dk0	37	29	-	-	HYPH
cord-259738-yuqc6dk0	37	30	cells	cell	NNS
cord-259738-yuqc6dk0	37	31	(	(	-LRB-
cord-259738-yuqc6dk0	37	32	Morgan	Morgan	NNP
cord-259738-yuqc6dk0	37	33	et	et	NNP
cord-259738-yuqc6dk0	37	34	al	al	NNP
cord-259738-yuqc6dk0	37	35	.	.	NNP
cord-259738-yuqc6dk0	37	36	,	,	,
cord-259738-yuqc6dk0	37	37	1976	1976	CD
cord-259738-yuqc6dk0	37	38	)	)	-RRB-
cord-259738-yuqc6dk0	37	39	.	.	.
cord-259738-yuqc6dk0	38	1	It	-PRON-	PRP
cord-259738-yuqc6dk0	38	2	is	be	VBZ
cord-259738-yuqc6dk0	38	3	secreted	secrete	VBN
cord-259738-yuqc6dk0	38	4	by	by	IN
cord-259738-yuqc6dk0	38	5	mature	mature	JJ
cord-259738-yuqc6dk0	38	6	T	t	NN
cord-259738-yuqc6dk0	38	7	-	-	HYPH
cord-259738-yuqc6dk0	38	8	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	38	9	upon	upon	IN
cord-259738-yuqc6dk0	38	10	stimulation	stimulation	NN
cord-259738-yuqc6dk0	38	11	and	and	CC
cord-259738-yuqc6dk0	38	12	certain	certain	JJ
cord-259738-yuqc6dk0	38	13	T	t	NN
cord-259738-yuqc6dk0	38	14	-	-	HYPH
cord-259738-yuqc6dk0	38	15	cell	cell	NN
cord-259738-yuqc6dk0	38	16	lymphoma	lymphoma	NN
cord-259738-yuqc6dk0	38	17	cell	cell	NN
cord-259738-yuqc6dk0	38	18	lines	line	NNS
cord-259738-yuqc6dk0	38	19	constitutively	constitutively	RB
cord-259738-yuqc6dk0	38	20	.	.	.
cord-259738-yuqc6dk0	39	1	Plasmid	Plasmid	NNP
cord-259738-yuqc6dk0	39	2	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	39	3	has	have	VBZ
cord-259738-yuqc6dk0	39	4	been	be	VBN
cord-259738-yuqc6dk0	39	5	investigated	investigate	VBN
cord-259738-yuqc6dk0	39	6	as	as	IN
cord-259738-yuqc6dk0	39	7	a	a	DT
cord-259738-yuqc6dk0	39	8	potential	potential	JJ
cord-259738-yuqc6dk0	39	9	vaccine	vaccine	NN
cord-259738-yuqc6dk0	39	10	adjuvant	adjuvant	NN
cord-259738-yuqc6dk0	39	11	in	in	IN
cord-259738-yuqc6dk0	39	12	several	several	JJ
cord-259738-yuqc6dk0	39	13	studies	study	NNS
cord-259738-yuqc6dk0	39	14	and	and	CC
cord-259738-yuqc6dk0	39	15	has	have	VBZ
cord-259738-yuqc6dk0	39	16	been	be	VBN
cord-259738-yuqc6dk0	39	17	shown	show	VBN
cord-259738-yuqc6dk0	39	18	to	to	TO
cord-259738-yuqc6dk0	39	19	increase	increase	VB
cord-259738-yuqc6dk0	39	20	DNA	dna	NN
cord-259738-yuqc6dk0	39	21	vaccine	vaccine	NN
cord-259738-yuqc6dk0	39	22	protective	protective	JJ
cord-259738-yuqc6dk0	39	23	immunity	immunity	NN
cord-259738-yuqc6dk0	39	24	against	against	IN
cord-259738-yuqc6dk0	39	25	pathogens	pathogen	NNS
cord-259738-yuqc6dk0	39	26	(	(	-LRB-
cord-259738-yuqc6dk0	39	27	Diane	Diane	NNP
cord-259738-yuqc6dk0	39	28	and	and	CC
cord-259738-yuqc6dk0	39	29	Carlos	Carlos	NNP
cord-259738-yuqc6dk0	39	30	,	,	,
cord-259738-yuqc6dk0	39	31	2004	2004	CD
cord-259738-yuqc6dk0	39	32	;	;	:
cord-259738-yuqc6dk0	39	33	Rompato	Rompato	NNP
cord-259738-yuqc6dk0	39	34	et	et	NNP
cord-259738-yuqc6dk0	39	35	al	al	NNP
cord-259738-yuqc6dk0	39	36	.	.	NNP
cord-259738-yuqc6dk0	39	37	,	,	,
cord-259738-yuqc6dk0	39	38	2006	2006	CD
cord-259738-yuqc6dk0	39	39	;	;	.
cord-259738-yuqc6dk0	40	1	Bu	Bu	NNP
cord-259738-yuqc6dk0	40	2	et	et	FW
cord-259738-yuqc6dk0	40	3	al	al	NNP
cord-259738-yuqc6dk0	40	4	.	.	NNP
cord-259738-yuqc6dk0	40	5	,	,	,
cord-259738-yuqc6dk0	40	6	2003	2003	CD
cord-259738-yuqc6dk0	40	7	)	)	-RRB-
cord-259738-yuqc6dk0	40	8	.	.	.
cord-259738-yuqc6dk0	41	1	In	in	IN
cord-259738-yuqc6dk0	41	2	the	the	DT
cord-259738-yuqc6dk0	41	3	present	present	JJ
cord-259738-yuqc6dk0	41	4	study	study	NN
cord-259738-yuqc6dk0	41	5	,	,	,
cord-259738-yuqc6dk0	41	6	a	a	DT
cord-259738-yuqc6dk0	41	7	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	41	8	plasmid	plasmid	NN
cord-259738-yuqc6dk0	41	9	encoding	encode	VBG
cord-259738-yuqc6dk0	41	10	the	the	DT
cord-259738-yuqc6dk0	41	11	nucleocapsid	nucleocapsid	NNP
cord-259738-yuqc6dk0	41	12	protein	protein	NN
cord-259738-yuqc6dk0	41	13	and	and	CC
cord-259738-yuqc6dk0	41	14	immune	immune	NN
cord-259738-yuqc6dk0	41	15	-	-	HYPH
cord-259738-yuqc6dk0	41	16	stimulatory	stimulatory	JJ
cord-259738-yuqc6dk0	41	17	interleukin-2	interleukin-2	NN
cord-259738-yuqc6dk0	41	18	has	have	VBZ
cord-259738-yuqc6dk0	41	19	been	be	VBN
cord-259738-yuqc6dk0	41	20	constructed	construct	VBN
cord-259738-yuqc6dk0	41	21	and	and	CC
cord-259738-yuqc6dk0	41	22	its	-PRON-	PRP$
cord-259738-yuqc6dk0	41	23	immunogenicity	immunogenicity	NN
cord-259738-yuqc6dk0	41	24	and	and	CC
cord-259738-yuqc6dk0	41	25	protective	protective	JJ
cord-259738-yuqc6dk0	41	26	effect	effect	NN
cord-259738-yuqc6dk0	41	27	in	in	IN
cord-259738-yuqc6dk0	41	28	chickens	chicken	NNS
cord-259738-yuqc6dk0	41	29	has	have	VBZ
cord-259738-yuqc6dk0	41	30	been	be	VBN
cord-259738-yuqc6dk0	41	31	evaluated	evaluate	VBN
cord-259738-yuqc6dk0	41	32	.	.	.
cord-259738-yuqc6dk0	42	1	It	-PRON-	PRP
cord-259738-yuqc6dk0	42	2	has	have	VBZ
cord-259738-yuqc6dk0	42	3	been	be	VBN
cord-259738-yuqc6dk0	42	4	shown	show	VBN
cord-259738-yuqc6dk0	42	5	that	that	IN
cord-259738-yuqc6dk0	42	6	the	the	DT
cord-259738-yuqc6dk0	42	7	delivery	delivery	NN
cord-259738-yuqc6dk0	42	8	of	of	IN
cord-259738-yuqc6dk0	42	9	a	a	DT
cord-259738-yuqc6dk0	42	10	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	42	11	plasmid	plasmid	NN
cord-259738-yuqc6dk0	42	12	containing	contain	VBG
cord-259738-yuqc6dk0	42	13	N	n	CD
cord-259738-yuqc6dk0	42	14	gene	gene	NN
cord-259738-yuqc6dk0	42	15	and	and	CC
cord-259738-yuqc6dk0	42	16	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	42	17	can	can	MD
cord-259738-yuqc6dk0	42	18	accelerate	accelerate	VB
cord-259738-yuqc6dk0	42	19	specific	specific	JJ
cord-259738-yuqc6dk0	42	20	antibody	antibody	NN
cord-259738-yuqc6dk0	42	21	induction	induction	NN
cord-259738-yuqc6dk0	42	22	with	with	IN
cord-259738-yuqc6dk0	42	23	an	an	DT
cord-259738-yuqc6dk0	42	24	increase	increase	NN
cord-259738-yuqc6dk0	42	25	T	t	NN
cord-259738-yuqc6dk0	42	26	-	-	HYPH
cord-259738-yuqc6dk0	42	27	cell	cell	NN
cord-259738-yuqc6dk0	42	28	responses	response	NNS
cord-259738-yuqc6dk0	42	29	.	.	.
cord-259738-yuqc6dk0	43	1	The	the	DT
cord-259738-yuqc6dk0	43	2	use	use	NN
cord-259738-yuqc6dk0	43	3	of	of	IN
cord-259738-yuqc6dk0	43	4	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	43	5	vector	vector	NN
cord-259738-yuqc6dk0	43	6	may	may	MD
cord-259738-yuqc6dk0	43	7	enable	enable	VB
cord-259738-yuqc6dk0	43	8	more	more	RBR
cord-259738-yuqc6dk0	43	9	efficient	efficient	JJ
cord-259738-yuqc6dk0	43	10	delivery	delivery	NN
cord-259738-yuqc6dk0	43	11	of	of	IN
cord-259738-yuqc6dk0	43	12	both	both	DT
cord-259738-yuqc6dk0	43	13	antigen	antigen	NN
cord-259738-yuqc6dk0	43	14	and	and	CC
cord-259738-yuqc6dk0	43	15	cytokine	cytokine	NN
cord-259738-yuqc6dk0	43	16	in	in	IN
cord-259738-yuqc6dk0	43	17	DNA	dna	NN
cord-259738-yuqc6dk0	43	18	vaccination	vaccination	NN
cord-259738-yuqc6dk0	43	19	and	and	CC
cord-259738-yuqc6dk0	43	20	promote	promote	VB
cord-259738-yuqc6dk0	43	21	synergistic	synergistic	JJ
cord-259738-yuqc6dk0	43	22	responses	response	NNS
cord-259738-yuqc6dk0	43	23	.	.	.
cord-259738-yuqc6dk0	44	1	The	the	DT
cord-259738-yuqc6dk0	44	2	specific	specific	JJ
cord-259738-yuqc6dk0	44	3	-	-	HYPH
cord-259738-yuqc6dk0	44	4	pathogen	pathogen	NN
cord-259738-yuqc6dk0	44	5	-	-	HYPH
cord-259738-yuqc6dk0	44	6	free	free	JJ
cord-259738-yuqc6dk0	44	7	(	(	-LRB-
cord-259738-yuqc6dk0	44	8	SPF	SPF	NNP
cord-259738-yuqc6dk0	44	9	)	)	-RRB-
cord-259738-yuqc6dk0	44	10	chicken	chicken	NN
cord-259738-yuqc6dk0	44	11	embryos	embryo	NNS
cord-259738-yuqc6dk0	44	12	were	be	VBD
cord-259738-yuqc6dk0	44	13	purchased	purchase	VBN
cord-259738-yuqc6dk0	44	14	from	from	IN
cord-259738-yuqc6dk0	44	15	Shangdong	Shangdong	NNP
cord-259738-yuqc6dk0	44	16	Institute	Institute	NNP
cord-259738-yuqc6dk0	44	17	of	of	IN
cord-259738-yuqc6dk0	44	18	Poultry	Poultry	NNP
cord-259738-yuqc6dk0	44	19	Science	Science	NNP
cord-259738-yuqc6dk0	44	20	,	,	,
cord-259738-yuqc6dk0	44	21	Shandong	Shandong	NNP
cord-259738-yuqc6dk0	44	22	,	,	,
cord-259738-yuqc6dk0	44	23	PR	PR	NNP
cord-259738-yuqc6dk0	44	24	China	China	NNP
cord-259738-yuqc6dk0	44	25	.	.	.
cord-259738-yuqc6dk0	45	1	Chickens	chicken	NNS
cord-259738-yuqc6dk0	45	2	were	be	VBD
cord-259738-yuqc6dk0	45	3	hatched	hatch	VBN
cord-259738-yuqc6dk0	45	4	and	and	CC
cord-259738-yuqc6dk0	45	5	housed	house	VBN
cord-259738-yuqc6dk0	45	6	in	in	IN
cord-259738-yuqc6dk0	45	7	a	a	DT
cord-259738-yuqc6dk0	45	8	specificpathogen	specificpathogen	NN
cord-259738-yuqc6dk0	45	9	-	-	HYPH
cord-259738-yuqc6dk0	45	10	free	free	JJ
cord-259738-yuqc6dk0	45	11	environment	environment	NN
cord-259738-yuqc6dk0	45	12	at	at	IN
cord-259738-yuqc6dk0	45	13	the	the	DT
cord-259738-yuqc6dk0	45	14	Laboratory	Laboratory	NNP
cord-259738-yuqc6dk0	45	15	Animal	Animal	NNP
cord-259738-yuqc6dk0	45	16	and	and	CC
cord-259738-yuqc6dk0	45	17	Resources	Resources	NNPS
cord-259738-yuqc6dk0	45	18	Facility	Facility	NNP
cord-259738-yuqc6dk0	45	19	,	,	,
cord-259738-yuqc6dk0	45	20	Sichuan	Sichuan	NNP
cord-259738-yuqc6dk0	45	21	University	University	NNP
cord-259738-yuqc6dk0	45	22	.	.	.
cord-259738-yuqc6dk0	46	1	The	the	DT
cord-259738-yuqc6dk0	46	2	nephropathogenic	nephropathogenic	JJ
cord-259738-yuqc6dk0	46	3	strain	strain	NN
cord-259738-yuqc6dk0	46	4	of	of	IN
cord-259738-yuqc6dk0	46	5	IBV	IBV	NNP
cord-259738-yuqc6dk0	46	6	,	,	,
cord-259738-yuqc6dk0	46	7	SAIBk	SAIBk	NNP
cord-259738-yuqc6dk0	46	8	strain	strain	NN
cord-259738-yuqc6dk0	46	9	was	be	VBD
cord-259738-yuqc6dk0	46	10	propagated	propagate	VBN
cord-259738-yuqc6dk0	46	11	in	in	IN
cord-259738-yuqc6dk0	46	12	the	the	DT
cord-259738-yuqc6dk0	46	13	allantoic	allantoic	NNP
cord-259738-yuqc6dk0	46	14	cavities	cavity	NNS
cord-259738-yuqc6dk0	46	15	of	of	IN
cord-259738-yuqc6dk0	46	16	10-day	10-day	CD
cord-259738-yuqc6dk0	46	17	-	-	HYPH
cord-259738-yuqc6dk0	46	18	old	old	JJ
cord-259738-yuqc6dk0	46	19	SPF	SPF	NNP
cord-259738-yuqc6dk0	46	20	embryonated	embryonate	VBN
cord-259738-yuqc6dk0	46	21	chicken	chicken	NN
cord-259738-yuqc6dk0	46	22	eggs	egg	NNS
cord-259738-yuqc6dk0	46	23	,	,	,
cord-259738-yuqc6dk0	46	24	and	and	CC
cord-259738-yuqc6dk0	46	25	then	then	RB
cord-259738-yuqc6dk0	46	26	harvested	harvest	VBD
cord-259738-yuqc6dk0	46	27	allantoic	allantoic	NNP
cord-259738-yuqc6dk0	46	28	fluid	fluid	NNP
cord-259738-yuqc6dk0	46	29	36	36	CD
cord-259738-yuqc6dk0	46	30	h	h	NN
cord-259738-yuqc6dk0	46	31	post	post	NN
cord-259738-yuqc6dk0	46	32	-	-	NN
cord-259738-yuqc6dk0	46	33	inoculation	inoculation	NN
cord-259738-yuqc6dk0	46	34	.	.	.
cord-259738-yuqc6dk0	47	1	The	the	DT
cord-259738-yuqc6dk0	47	2	50	50	CD
cord-259738-yuqc6dk0	47	3	%	%	NN
cord-259738-yuqc6dk0	47	4	chicken	chicken	NN
cord-259738-yuqc6dk0	47	5	infection	infection	NN
cord-259738-yuqc6dk0	47	6	dose	dose	NN
cord-259738-yuqc6dk0	47	7	(	(	-LRB-
cord-259738-yuqc6dk0	47	8	EID	EID	NNP
cord-259738-yuqc6dk0	47	9	50	50	CD
cord-259738-yuqc6dk0	47	10	)	)	-RRB-
cord-259738-yuqc6dk0	47	11	was	be	VBD
cord-259738-yuqc6dk0	47	12	determined	determine	VBN
cord-259738-yuqc6dk0	47	13	by	by	IN
cord-259738-yuqc6dk0	47	14	inoculating	inoculate	VBG
cord-259738-yuqc6dk0	47	15	serial	serial	JJ
cord-259738-yuqc6dk0	47	16	10fold	10fold	CD
cord-259738-yuqc6dk0	47	17	dilutions	dilution	NNS
cord-259738-yuqc6dk0	47	18	of	of	IN
cord-259738-yuqc6dk0	47	19	virus	virus	NN
cord-259738-yuqc6dk0	47	20	into	into	IN
cord-259738-yuqc6dk0	47	21	10-day	10-day	CD
cord-259738-yuqc6dk0	47	22	-	-	HYPH
cord-259738-yuqc6dk0	47	23	old	old	JJ
cord-259738-yuqc6dk0	47	24	SPF	SPF	NNP
cord-259738-yuqc6dk0	47	25	embryonated	embryonate	VBN
cord-259738-yuqc6dk0	47	26	chicken	chicken	NN
cord-259738-yuqc6dk0	47	27	eggs	egg	NNS
cord-259738-yuqc6dk0	47	28	.	.	.
cord-259738-yuqc6dk0	48	1	Vero	Vero	NNP
cord-259738-yuqc6dk0	48	2	cells	cell	NNS
cord-259738-yuqc6dk0	48	3	were	be	VBD
cord-259738-yuqc6dk0	48	4	cultured	cultured	JJ
cord-259738-yuqc6dk0	48	5	in	in	IN
cord-259738-yuqc6dk0	48	6	Dulbecco	Dulbecco	NNP
cord-259738-yuqc6dk0	48	7	's	's	POS
cord-259738-yuqc6dk0	48	8	modified	modify	VBN
cord-259738-yuqc6dk0	48	9	Eagle	Eagle	NNP
cord-259738-yuqc6dk0	48	10	's	's	POS
cord-259738-yuqc6dk0	48	11	medium	medium	NN
cord-259738-yuqc6dk0	48	12	(	(	-LRB-
cord-259738-yuqc6dk0	48	13	Gibco	Gibco	NNP
cord-259738-yuqc6dk0	48	14	)	)	-RRB-
cord-259738-yuqc6dk0	48	15	supplemented	supplement	VBD
cord-259738-yuqc6dk0	48	16	with	with	IN
cord-259738-yuqc6dk0	48	17	10	10	CD
cord-259738-yuqc6dk0	48	18	%	%	NN
cord-259738-yuqc6dk0	48	19	FBS	FBS	NNP
cord-259738-yuqc6dk0	48	20	,	,	,
cord-259738-yuqc6dk0	48	21	100	100	CD
cord-259738-yuqc6dk0	48	22	U	u	NN
cord-259738-yuqc6dk0	48	23	/	/	SYM
cord-259738-yuqc6dk0	48	24	ml	ml	NN
cord-259738-yuqc6dk0	48	25	penicillin	penicillin	NN
cord-259738-yuqc6dk0	48	26	and	and	CC
cord-259738-yuqc6dk0	48	27	100	100	CD
cord-259738-yuqc6dk0	48	28	g	g	NN
cord-259738-yuqc6dk0	48	29	/	/	SYM
cord-259738-yuqc6dk0	48	30	ml	ml	NN
cord-259738-yuqc6dk0	48	31	streptomycin	streptomycin	NNS
cord-259738-yuqc6dk0	48	32	at	at	IN
cord-259738-yuqc6dk0	48	33	pH	pH	NNP
cord-259738-yuqc6dk0	48	34	7.2	7.2	CD
cord-259738-yuqc6dk0	48	35	and	and	CC
cord-259738-yuqc6dk0	48	36	were	be	VBD
cord-259738-yuqc6dk0	48	37	kept	keep	VBN
cord-259738-yuqc6dk0	48	38	at	at	IN
cord-259738-yuqc6dk0	48	39	37	37	CD
cord-259738-yuqc6dk0	48	40	•	•	NN
cord-259738-yuqc6dk0	48	41	C	c	NN
cord-259738-yuqc6dk0	48	42	with	with	IN
cord-259738-yuqc6dk0	48	43	5	5	CD
cord-259738-yuqc6dk0	48	44	%	%	NN
cord-259738-yuqc6dk0	48	45	carbon	carbon	NN
cord-259738-yuqc6dk0	48	46	dioxide	dioxide	NN
cord-259738-yuqc6dk0	48	47	.	.	.
cord-259738-yuqc6dk0	49	1	The	the	DT
cord-259738-yuqc6dk0	49	2	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	49	3	-	-	HYPH
cord-259738-yuqc6dk0	49	4	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	49	5	/	/	SYM
cord-259738-yuqc6dk0	49	6	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	49	7	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	49	8	plasmid	plasmid	NN
cord-259738-yuqc6dk0	49	9	which	which	WDT
cord-259738-yuqc6dk0	49	10	was	be	VBD
cord-259738-yuqc6dk0	49	11	constructed	construct	VBN
cord-259738-yuqc6dk0	49	12	and	and	CC
cord-259738-yuqc6dk0	49	13	identified	identify	VBN
cord-259738-yuqc6dk0	49	14	previously	previously	RB
cord-259738-yuqc6dk0	49	15	,	,	,
cord-259738-yuqc6dk0	49	16	was	be	VBD
cord-259738-yuqc6dk0	49	17	used	use	VBN
cord-259738-yuqc6dk0	49	18	to	to	TO
cord-259738-yuqc6dk0	49	19	construct	construct	VB
cord-259738-yuqc6dk0	49	20	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	49	21	and	and	CC
cord-259738-yuqc6dk0	49	22	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	49	23	DNA	DNA	NNP
cord-259738-yuqc6dk0	49	24	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	49	25	.	.	.
cord-259738-yuqc6dk0	50	1	This	this	DT
cord-259738-yuqc6dk0	50	2	plasmid	plasmid	NN
cord-259738-yuqc6dk0	50	3	contained	contain	VBD
cord-259738-yuqc6dk0	50	4	two	two	CD
cord-259738-yuqc6dk0	50	5	fluorescent	fluorescent	JJ
cord-259738-yuqc6dk0	50	6	protein	protein	NN
cord-259738-yuqc6dk0	50	7	genes	gene	NNS
cord-259738-yuqc6dk0	50	8	which	which	WDT
cord-259738-yuqc6dk0	50	9	were	be	VBD
cord-259738-yuqc6dk0	50	10	inserted	insert	VBN
cord-259738-yuqc6dk0	50	11	into	into	IN
cord-259738-yuqc6dk0	50	12	the	the	DT
cord-259738-yuqc6dk0	50	13	multiple	multiple	JJ
cord-259738-yuqc6dk0	50	14	cloning	cloning	NN
cord-259738-yuqc6dk0	50	15	sites	site	NNS
cord-259738-yuqc6dk0	50	16	(	(	-LRB-
cord-259738-yuqc6dk0	50	17	MCS	MCS	NNP
cord-259738-yuqc6dk0	50	18	)	)	-RRB-
cord-259738-yuqc6dk0	50	19	located	locate	VBN
cord-259738-yuqc6dk0	50	20	on	on	IN
cord-259738-yuqc6dk0	50	21	either	either	DT
cord-259738-yuqc6dk0	50	22	side	side	NN
cord-259738-yuqc6dk0	50	23	of	of	IN
cord-259738-yuqc6dk0	50	24	the	the	DT
cord-259738-yuqc6dk0	50	25	internal	internal	JJ
cord-259738-yuqc6dk0	50	26	ribosome	ribosome	NN
cord-259738-yuqc6dk0	50	27	entry	entry	NN
cord-259738-yuqc6dk0	50	28	site	site	NN
cord-259738-yuqc6dk0	50	29	from	from	IN
cord-259738-yuqc6dk0	50	30	the	the	DT
cord-259738-yuqc6dk0	50	31	encephalomyocarditis	encephalomyocarditis	NNP
cord-259738-yuqc6dk0	50	32	virus	virus	NN
cord-259738-yuqc6dk0	50	33	(	(	-LRB-
cord-259738-yuqc6dk0	50	34	ECMV	ECMV	NNP
cord-259738-yuqc6dk0	50	35	)	)	-RRB-
cord-259738-yuqc6dk0	50	36	.	.	.
cord-259738-yuqc6dk0	51	1	The	the	DT
cord-259738-yuqc6dk0	51	2	IBV	IBV	NNP
cord-259738-yuqc6dk0	51	3	N	N	NNP
cord-259738-yuqc6dk0	51	4	gene	gene	NN
cord-259738-yuqc6dk0	51	5	was	be	VBD
cord-259738-yuqc6dk0	51	6	subcloned	subclone	VBN
cord-259738-yuqc6dk0	51	7	from	from	IN
cord-259738-yuqc6dk0	51	8	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	51	9	plasmid	plasmid	NN
cord-259738-yuqc6dk0	51	10	pIBVN	pibvn	NN
cord-259738-yuqc6dk0	51	11	into	into	IN
cord-259738-yuqc6dk0	51	12	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	51	13	of	of	IN
cord-259738-yuqc6dk0	51	14	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	51	15	-	-	HYPH
cord-259738-yuqc6dk0	51	16	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	51	17	/	/	SYM
cord-259738-yuqc6dk0	51	18	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	51	19	between	between	IN
cord-259738-yuqc6dk0	51	20	NheI	NheI	NNP
cord-259738-yuqc6dk0	51	21	and	and	CC
cord-259738-yuqc6dk0	51	22	XhoI	XhoI	NNP
cord-259738-yuqc6dk0	51	23	restriction	restriction	NN
cord-259738-yuqc6dk0	51	24	sites	site	NNS
cord-259738-yuqc6dk0	51	25	,	,	,
cord-259738-yuqc6dk0	51	26	and	and	CC
cord-259738-yuqc6dk0	51	27	generated	generate	VBD
cord-259738-yuqc6dk0	51	28	the	the	DT
cord-259738-yuqc6dk0	51	29	plasmid	plasmid	NN
cord-259738-yuqc6dk0	51	30	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	51	31	-	-	HYPH
cord-259738-yuqc6dk0	51	32	N	N	NNP
cord-259738-yuqc6dk0	51	33	/	/	SYM
cord-259738-yuqc6dk0	51	34	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	51	35	.	.	.
cord-259738-yuqc6dk0	52	1	The	the	DT
cord-259738-yuqc6dk0	52	2	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	52	3	gene	gene	NN
cord-259738-yuqc6dk0	52	4	was	be	VBD
cord-259738-yuqc6dk0	52	5	amplified	amplify	VBN
cord-259738-yuqc6dk0	52	6	by	by	IN
cord-259738-yuqc6dk0	52	7	PCR	PCR	NNP
cord-259738-yuqc6dk0	52	8	from	from	IN
cord-259738-yuqc6dk0	52	9	the	the	DT
cord-259738-yuqc6dk0	52	10	plasmid	plasmid	NN
cord-259738-yuqc6dk0	52	11	pDNAIL-2	pDNAIL-2	NNP
cord-259738-yuqc6dk0	52	12	as	as	IN
cord-259738-yuqc6dk0	52	13	a	a	DT
cord-259738-yuqc6dk0	52	14	template	template	NN
cord-259738-yuqc6dk0	52	15	.	.	.
cord-259738-yuqc6dk0	53	1	The	the	DT
cord-259738-yuqc6dk0	53	2	forward	forward	JJ
cord-259738-yuqc6dk0	53	3	primer	primer	NN
cord-259738-yuqc6dk0	53	4	(	(	-LRB-
cord-259738-yuqc6dk0	53	5	5	5	CD
cord-259738-yuqc6dk0	53	6	-CCAGGATCCACCATGATGTG	-ccaggatccaccatgatgtg	NN
cord-259738-yuqc6dk0	53	7	AAG-3	aag-3	NN
cord-259738-yuqc6dk0	53	8	)	)	-RRB-
cord-259738-yuqc6dk0	53	9	and	and	CC
cord-259738-yuqc6dk0	53	10	reverse	reverse	VB
cord-259738-yuqc6dk0	53	11	primer	primer	NN
cord-259738-yuqc6dk0	53	12	(	(	-LRB-
cord-259738-yuqc6dk0	53	13	5	5	CD
cord-259738-yuqc6dk0	53	14	-GAAGCGGCCGCAGATTAGTTAGC-3	-GAAGCGGCCGCAGATTAGTTAGC-3	NNP
cord-259738-yuqc6dk0	53	15	)	)	-RRB-
cord-259738-yuqc6dk0	53	16	specific	specific	JJ
cord-259738-yuqc6dk0	53	17	for	for	IN
cord-259738-yuqc6dk0	53	18	the	the	DT
cord-259738-yuqc6dk0	53	19	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	53	20	gene	gene	NN
cord-259738-yuqc6dk0	53	21	were	be	VBD
cord-259738-yuqc6dk0	53	22	employed	employ	VBN
cord-259738-yuqc6dk0	53	23	.	.	.
cord-259738-yuqc6dk0	54	1	The	the	DT
cord-259738-yuqc6dk0	54	2	PCR	PCR	NNP
cord-259738-yuqc6dk0	54	3	product	product	NN
cord-259738-yuqc6dk0	54	4	of	of	IN
cord-259738-yuqc6dk0	54	5	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	54	6	was	be	VBD
cord-259738-yuqc6dk0	54	7	digested	digest	VBN
cord-259738-yuqc6dk0	54	8	with	with	IN
cord-259738-yuqc6dk0	54	9	BamHI	BamHI	NNP
cord-259738-yuqc6dk0	54	10	and	and	CC
cord-259738-yuqc6dk0	54	11	NotI	NotI	NNP
cord-259738-yuqc6dk0	54	12	and	and	CC
cord-259738-yuqc6dk0	54	13	ligated	ligate	VBD
cord-259738-yuqc6dk0	54	14	into	into	IN
cord-259738-yuqc6dk0	54	15	similarly	similarly	RB
cord-259738-yuqc6dk0	54	16	digested	digest	VBN
cord-259738-yuqc6dk0	54	17	pIRES	pires	NN
cord-259738-yuqc6dk0	54	18	-	-	HYPH
cord-259738-yuqc6dk0	54	19	N	N	NNP
cord-259738-yuqc6dk0	54	20	/	/	SYM
cord-259738-yuqc6dk0	54	21	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	54	22	,	,	,
cord-259738-yuqc6dk0	54	23	then	then	RB
cord-259738-yuqc6dk0	54	24	the	the	DT
cord-259738-yuqc6dk0	54	25	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	54	26	plasmid	plasmid	NN
cord-259738-yuqc6dk0	54	27	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	54	28	-	-	HYPH
cord-259738-yuqc6dk0	54	29	N	N	NNP
cord-259738-yuqc6dk0	54	30	/	/	SYM
cord-259738-yuqc6dk0	54	31	IL2	IL2	NNP
cord-259738-yuqc6dk0	54	32	was	be	VBD
cord-259738-yuqc6dk0	54	33	created	create	VBN
cord-259738-yuqc6dk0	54	34	.	.	.
cord-259738-yuqc6dk0	55	1	The	the	DT
cord-259738-yuqc6dk0	55	2	plasmid	plasmid	NN
cord-259738-yuqc6dk0	55	3	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	55	4	-	-	HYPH
cord-259738-yuqc6dk0	55	5	N	N	NNP
cord-259738-yuqc6dk0	55	6	/	/	SYM
cord-259738-yuqc6dk0	55	7	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	55	8	was	be	VBD
cord-259738-yuqc6dk0	55	9	digested	digest	VBN
cord-259738-yuqc6dk0	55	10	with	with	IN
cord-259738-yuqc6dk0	55	11	BamHI	BamHI	NNP
cord-259738-yuqc6dk0	55	12	and	and	CC
cord-259738-yuqc6dk0	55	13	NotI	NotI	NNP
cord-259738-yuqc6dk0	55	14	,	,	,
cord-259738-yuqc6dk0	55	15	gel	gel	NN
cord-259738-yuqc6dk0	55	16	purified	purify	VBN
cord-259738-yuqc6dk0	55	17	and	and	CC
cord-259738-yuqc6dk0	55	18	blunt	blunt	JJ
cord-259738-yuqc6dk0	55	19	ended	end	VBD
cord-259738-yuqc6dk0	55	20	to	to	TO
cord-259738-yuqc6dk0	55	21	create	create	VB
cord-259738-yuqc6dk0	55	22	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	55	23	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	55	24	-	-	HYPH
cord-259738-yuqc6dk0	55	25	N.	N.	NNP
cord-259738-yuqc6dk0	55	26	Identification	identification	NN
cord-259738-yuqc6dk0	55	27	of	of	IN
cord-259738-yuqc6dk0	55	28	the	the	DT
cord-259738-yuqc6dk0	55	29	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	55	30	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	55	31	was	be	VBD
cord-259738-yuqc6dk0	55	32	performed	perform	VBN
cord-259738-yuqc6dk0	55	33	by	by	IN
cord-259738-yuqc6dk0	55	34	double	double	JJ
cord-259738-yuqc6dk0	55	35	enzymes	enzyme	NNS
cord-259738-yuqc6dk0	55	36	digestion	digestion	NN
cord-259738-yuqc6dk0	55	37	and	and	CC
cord-259738-yuqc6dk0	55	38	DNA	dna	NN
cord-259738-yuqc6dk0	55	39	sequencing	sequencing	NN
cord-259738-yuqc6dk0	55	40	.	.	.
cord-259738-yuqc6dk0	56	1	Six	six	CD
cord-259738-yuqc6dk0	56	2	-	-	HYPH
cord-259738-yuqc6dk0	56	3	well	well	RB
cord-259738-yuqc6dk0	56	4	tissue	tissue	NN
cord-259738-yuqc6dk0	56	5	culture	culture	NN
cord-259738-yuqc6dk0	56	6	plates	plate	NNS
cord-259738-yuqc6dk0	56	7	were	be	VBD
cord-259738-yuqc6dk0	56	8	seeded	seed	VBN
cord-259738-yuqc6dk0	56	9	with	with	IN
cord-259738-yuqc6dk0	56	10	Vero	Vero	NNP
cord-259738-yuqc6dk0	56	11	cells	cell	NNS
cord-259738-yuqc6dk0	56	12	(	(	-LRB-
cord-259738-yuqc6dk0	56	13	10	10	CD
cord-259738-yuqc6dk0	56	14	6	6	CD
cord-259738-yuqc6dk0	56	15	/well	/well	,
cord-259738-yuqc6dk0	56	16	)	)	-RRB-
cord-259738-yuqc6dk0	56	17	,	,	,
cord-259738-yuqc6dk0	56	18	and	and	CC
cord-259738-yuqc6dk0	56	19	the	the	DT
cord-259738-yuqc6dk0	56	20	cells	cell	NNS
cord-259738-yuqc6dk0	56	21	were	be	VBD
cord-259738-yuqc6dk0	56	22	grown	grow	VBN
cord-259738-yuqc6dk0	56	23	until	until	IN
cord-259738-yuqc6dk0	56	24	they	-PRON-	PRP
cord-259738-yuqc6dk0	56	25	were	be	VBD
cord-259738-yuqc6dk0	56	26	about	about	RB
cord-259738-yuqc6dk0	56	27	70	70	CD
cord-259738-yuqc6dk0	56	28	%	%	NN
cord-259738-yuqc6dk0	56	29	confluent	confluent	JJ
cord-259738-yuqc6dk0	56	30	.	.	.
cord-259738-yuqc6dk0	57	1	The	the	DT
cord-259738-yuqc6dk0	57	2	purified	purify	VBN
cord-259738-yuqc6dk0	57	3	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	57	4	,	,	,
cord-259738-yuqc6dk0	57	5	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	57	6	-	-	HYPH
cord-259738-yuqc6dk0	57	7	N	N	NNP
cord-259738-yuqc6dk0	57	8	,	,	,
cord-259738-yuqc6dk0	57	9	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	57	10	-	-	HYPH
cord-259738-yuqc6dk0	57	11	N	N	NNP
cord-259738-yuqc6dk0	57	12	/	/	SYM
cord-259738-yuqc6dk0	57	13	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	57	14	and	and	CC
cord-259738-yuqc6dk0	57	15	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	57	16	were	be	VBD
cord-259738-yuqc6dk0	57	17	transfected	transfecte	VBN
cord-259738-yuqc6dk0	57	18	respectively	respectively	RB
cord-259738-yuqc6dk0	57	19	into	into	IN
cord-259738-yuqc6dk0	57	20	the	the	DT
cord-259738-yuqc6dk0	57	21	Vero	Vero	NNP
cord-259738-yuqc6dk0	57	22	cells	cell	NNS
cord-259738-yuqc6dk0	57	23	with	with	IN
cord-259738-yuqc6dk0	57	24	lipofectamine	lipofectamine	NN
cord-259738-yuqc6dk0	57	25	according	accord	VBG
cord-259738-yuqc6dk0	57	26	to	to	IN
cord-259738-yuqc6dk0	57	27	the	the	DT
cord-259738-yuqc6dk0	57	28	manufacturer	manufacturer	NN
cord-259738-yuqc6dk0	57	29	's	's	POS
cord-259738-yuqc6dk0	57	30	instructions	instruction	NNS
cord-259738-yuqc6dk0	57	31	(	(	-LRB-
cord-259738-yuqc6dk0	57	32	Invitrogen	Invitrogen	NNP
cord-259738-yuqc6dk0	57	33	,	,	,
cord-259738-yuqc6dk0	57	34	CA	CA	NNP
cord-259738-yuqc6dk0	57	35	,	,	,
cord-259738-yuqc6dk0	57	36	USA	USA	NNP
cord-259738-yuqc6dk0	57	37	)	)	-RRB-
cord-259738-yuqc6dk0	57	38	.	.	.
cord-259738-yuqc6dk0	58	1	The	the	DT
cord-259738-yuqc6dk0	58	2	expression	expression	NN
cord-259738-yuqc6dk0	58	3	products	product	NNS
cord-259738-yuqc6dk0	58	4	were	be	VBD
cord-259738-yuqc6dk0	58	5	identified	identify	VBN
cord-259738-yuqc6dk0	58	6	after	after	IN
cord-259738-yuqc6dk0	58	7	36	36	CD
cord-259738-yuqc6dk0	58	8	-	-	SYM
cord-259738-yuqc6dk0	58	9	48	48	CD
cord-259738-yuqc6dk0	58	10	h.	h.	NN
cord-259738-yuqc6dk0	59	1	The	the	DT
cord-259738-yuqc6dk0	59	2	transfected	transfecte	VBN
cord-259738-yuqc6dk0	59	3	cells	cell	NNS
cord-259738-yuqc6dk0	59	4	were	be	VBD
cord-259738-yuqc6dk0	59	5	harvested	harvest	VBN
cord-259738-yuqc6dk0	59	6	after	after	IN
cord-259738-yuqc6dk0	59	7	36	36	CD
cord-259738-yuqc6dk0	59	8	h	h	NN
cord-259738-yuqc6dk0	59	9	and	and	CC
cord-259738-yuqc6dk0	59	10	total	total	JJ
cord-259738-yuqc6dk0	59	11	cellular	cellular	JJ
cord-259738-yuqc6dk0	59	12	RNA	RNA	NNP
cord-259738-yuqc6dk0	59	13	was	be	VBD
cord-259738-yuqc6dk0	59	14	prepared	prepare	VBN
cord-259738-yuqc6dk0	59	15	from	from	IN
cord-259738-yuqc6dk0	59	16	the	the	DT
cord-259738-yuqc6dk0	59	17	transfected	transfecte	VBN
cord-259738-yuqc6dk0	59	18	cells	cell	NNS
cord-259738-yuqc6dk0	59	19	by	by	IN
cord-259738-yuqc6dk0	59	20	TRIZOL	TRIZOL	NNP
cord-259738-yuqc6dk0	59	21	reagent	reagent	NN
cord-259738-yuqc6dk0	59	22	(	(	-LRB-
cord-259738-yuqc6dk0	59	23	Gibco	Gibco	NNP
cord-259738-yuqc6dk0	59	24	BRL	BRL	NNP
cord-259738-yuqc6dk0	59	25	,	,	,
cord-259738-yuqc6dk0	59	26	USA	USA	NNP
cord-259738-yuqc6dk0	59	27	)	)	-RRB-
cord-259738-yuqc6dk0	59	28	.	.	.
cord-259738-yuqc6dk0	60	1	The	the	DT
cord-259738-yuqc6dk0	60	2	transcription	transcription	NN
cord-259738-yuqc6dk0	60	3	products	product	NNS
cord-259738-yuqc6dk0	60	4	were	be	VBD
cord-259738-yuqc6dk0	60	5	detected	detect	VBN
cord-259738-yuqc6dk0	60	6	by	by	IN
cord-259738-yuqc6dk0	60	7	reverse	reverse	JJ
cord-259738-yuqc6dk0	60	8	-	-	HYPH
cord-259738-yuqc6dk0	60	9	transcription	transcription	NN
cord-259738-yuqc6dk0	60	10	polymerase	polymerase	NN
cord-259738-yuqc6dk0	60	11	chain	chain	NN
cord-259738-yuqc6dk0	60	12	reaction	reaction	NN
cord-259738-yuqc6dk0	60	13	(	(	-LRB-
cord-259738-yuqc6dk0	60	14	RT	RT	NNP
cord-259738-yuqc6dk0	60	15	-	-	HYPH
cord-259738-yuqc6dk0	60	16	PCR	PCR	NNP
cord-259738-yuqc6dk0	60	17	)	)	-RRB-
cord-259738-yuqc6dk0	60	18	with	with	IN
cord-259738-yuqc6dk0	60	19	specific	specific	JJ
cord-259738-yuqc6dk0	60	20	primer	primer	NN
cord-259738-yuqc6dk0	60	21	sets	set	NNS
cord-259738-yuqc6dk0	60	22	for	for	IN
cord-259738-yuqc6dk0	60	23	the	the	DT
cord-259738-yuqc6dk0	60	24	N	n	CD
cord-259738-yuqc6dk0	60	25	gene	gene	NN
cord-259738-yuqc6dk0	60	26	and	and	CC
cord-259738-yuqc6dk0	60	27	IL-2	il-2	NN
cord-259738-yuqc6dk0	60	28	as	as	IN
cord-259738-yuqc6dk0	60	29	listed	list	VBN
cord-259738-yuqc6dk0	60	30	.	.	.
cord-259738-yuqc6dk0	61	1	N	N	NNP
cord-259738-yuqc6dk0	61	2	forward	forward	NNP
cord-259738-yuqc6dk0	61	3	primer	primer	NN
cord-259738-yuqc6dk0	61	4	:	:	:
cord-259738-yuqc6dk0	61	5	5	5	CD
cord-259738-yuqc6dk0	61	6	-CATCTCGAGTCTT	-catctcgagtctt	NN
cord-259738-yuqc6dk0	61	7	-	-	:
cord-259738-yuqc6dk0	61	8	TTATCATGGCAAGC-3	ttatcatggcaagc-3	JJ
cord-259738-yuqc6dk0	61	9	,	,	,
cord-259738-yuqc6dk0	61	10	reverse	reverse	JJ
cord-259738-yuqc6dk0	61	11	primer	primer	NN
cord-259738-yuqc6dk0	61	12	:	:	:
cord-259738-yuqc6dk0	61	13	5	5	LS
cord-259738-yuqc6dk0	61	14	-GGCGAATTCA	-GGCGAATTCA	,
cord-259738-yuqc6dk0	61	15	-	-	:
cord-259738-yuqc6dk0	61	16	TTAGAGTTCATTTTCAC-3	TTAGAGTTCATTTTCAC-3	NNP
cord-259738-yuqc6dk0	61	17	;	;	:
cord-259738-yuqc6dk0	61	18	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	62	1	forward	forward	NNP
cord-259738-yuqc6dk0	62	2	primer	primer	NN
cord-259738-yuqc6dk0	62	3	:	:	:
cord-259738-yuqc6dk0	62	4	5	5	CD
cord-259738-yuqc6dk0	62	5	-CCAGGATCCACCATGATGTGCAAAG-3	-ccaggatccaccatgatgtgcaaag-3	SYM
cord-259738-yuqc6dk0	62	6	,	,	,
cord-259738-yuqc6dk0	62	7	reverse	reverse	JJ
cord-259738-yuqc6dk0	62	8	primer	primer	NN
cord-259738-yuqc6dk0	62	9	:	:	:
cord-259738-yuqc6dk0	62	10	5	5	CD
cord-259738-yuqc6dk0	62	11	-GAAGCGGCCGCAGATTAGTTAGC-3	-GAAGCGGCCGCAGATTAGTTAGC-3	NNP
cord-259738-yuqc6dk0	62	12	.	.	.
cord-259738-yuqc6dk0	63	1	The	the	DT
cord-259738-yuqc6dk0	63	2	medium	medium	NN
cord-259738-yuqc6dk0	63	3	was	be	VBD
cord-259738-yuqc6dk0	63	4	aspirated	aspirate	VBN
cord-259738-yuqc6dk0	63	5	48	48	CD
cord-259738-yuqc6dk0	63	6	h	h	NN
cord-259738-yuqc6dk0	63	7	after	after	IN
cord-259738-yuqc6dk0	63	8	transfection	transfection	NN
cord-259738-yuqc6dk0	63	9	,	,	,
cord-259738-yuqc6dk0	63	10	and	and	CC
cord-259738-yuqc6dk0	63	11	the	the	DT
cord-259738-yuqc6dk0	63	12	cells	cell	NNS
cord-259738-yuqc6dk0	63	13	were	be	VBD
cord-259738-yuqc6dk0	63	14	washed	wash	VBN
cord-259738-yuqc6dk0	63	15	once	once	RB
cord-259738-yuqc6dk0	63	16	with	with	IN
cord-259738-yuqc6dk0	63	17	phosphate	phosphate	NN
cord-259738-yuqc6dk0	63	18	-	-	HYPH
cord-259738-yuqc6dk0	63	19	buffered	buffer	VBN
cord-259738-yuqc6dk0	63	20	-	-	HYPH
cord-259738-yuqc6dk0	63	21	saline	saline	NN
cord-259738-yuqc6dk0	63	22	(	(	-LRB-
cord-259738-yuqc6dk0	63	23	PBS	PBS	NNP
cord-259738-yuqc6dk0	63	24	)	)	-RRB-
cord-259738-yuqc6dk0	63	25	,	,	,
cord-259738-yuqc6dk0	63	26	fixed	fix	VBN
cord-259738-yuqc6dk0	63	27	with	with	IN
cord-259738-yuqc6dk0	63	28	100	100	CD
cord-259738-yuqc6dk0	63	29	%	%	NN
cord-259738-yuqc6dk0	63	30	acetone	acetone	NN
cord-259738-yuqc6dk0	63	31	for	for	IN
cord-259738-yuqc6dk0	63	32	10	10	CD
cord-259738-yuqc6dk0	63	33	min	min	NN
cord-259738-yuqc6dk0	63	34	at	at	IN
cord-259738-yuqc6dk0	63	35	−20	−20	NNP
cord-259738-yuqc6dk0	63	36	•	•	NNP
cord-259738-yuqc6dk0	63	37	C	C	NNP
cord-259738-yuqc6dk0	63	38	,	,	,
cord-259738-yuqc6dk0	63	39	then	then	RB
cord-259738-yuqc6dk0	63	40	washed	wash	VBD
cord-259738-yuqc6dk0	63	41	three	three	CD
cord-259738-yuqc6dk0	63	42	times	time	NNS
cord-259738-yuqc6dk0	63	43	for	for	IN
cord-259738-yuqc6dk0	63	44	5	5	CD
cord-259738-yuqc6dk0	63	45	min	min	NN
cord-259738-yuqc6dk0	63	46	each	each	DT
cord-259738-yuqc6dk0	63	47	with	with	IN
cord-259738-yuqc6dk0	63	48	PBS	PBS	NNP
cord-259738-yuqc6dk0	63	49	.	.	.
cord-259738-yuqc6dk0	64	1	Thereafter	thereafter	RB
cord-259738-yuqc6dk0	64	2	,	,	,
cord-259738-yuqc6dk0	64	3	transfected	transfecte	VBN
cord-259738-yuqc6dk0	64	4	cells	cell	NNS
cord-259738-yuqc6dk0	64	5	were	be	VBD
cord-259738-yuqc6dk0	64	6	incubated	incubate	VBN
cord-259738-yuqc6dk0	64	7	at	at	IN
cord-259738-yuqc6dk0	64	8	37	37	CD
cord-259738-yuqc6dk0	64	9	•	•	NN
cord-259738-yuqc6dk0	64	10	C	c	NN
cord-259738-yuqc6dk0	64	11	for	for	IN
cord-259738-yuqc6dk0	64	12	1	1	CD
cord-259738-yuqc6dk0	64	13	h	h	NN
cord-259738-yuqc6dk0	64	14	with	with	IN
cord-259738-yuqc6dk0	64	15	the	the	DT
cord-259738-yuqc6dk0	64	16	antibody	antibody	NN
cord-259738-yuqc6dk0	64	17	,	,	,
cord-259738-yuqc6dk0	64	18	which	which	WDT
cord-259738-yuqc6dk0	64	19	was	be	VBD
cord-259738-yuqc6dk0	64	20	antiserum	antiserum	NN
cord-259738-yuqc6dk0	64	21	of	of	IN
cord-259738-yuqc6dk0	64	22	rabbit	rabbit	NN
cord-259738-yuqc6dk0	64	23	to	to	IN
cord-259738-yuqc6dk0	64	24	IBV	IBV	NNP
cord-259738-yuqc6dk0	64	25	.	.	.
cord-259738-yuqc6dk0	65	1	Then	then	RB
cord-259738-yuqc6dk0	65	2	the	the	DT
cord-259738-yuqc6dk0	65	3	cells	cell	NNS
cord-259738-yuqc6dk0	65	4	were	be	VBD
cord-259738-yuqc6dk0	65	5	washed	wash	VBN
cord-259738-yuqc6dk0	65	6	twice	twice	RB
cord-259738-yuqc6dk0	65	7	for	for	IN
cord-259738-yuqc6dk0	65	8	5	5	CD
cord-259738-yuqc6dk0	65	9	min	min	NN
cord-259738-yuqc6dk0	65	10	each	each	DT
cord-259738-yuqc6dk0	65	11	with	with	IN
cord-259738-yuqc6dk0	65	12	PBS	PBS	NNP
cord-259738-yuqc6dk0	65	13	and	and	CC
cord-259738-yuqc6dk0	65	14	incubated	incubate	VBN
cord-259738-yuqc6dk0	65	15	for	for	IN
cord-259738-yuqc6dk0	65	16	a	a	DT
cord-259738-yuqc6dk0	65	17	further	further	JJ
cord-259738-yuqc6dk0	65	18	1	1	CD
cord-259738-yuqc6dk0	65	19	h	h	NN
cord-259738-yuqc6dk0	65	20	at	at	IN
cord-259738-yuqc6dk0	65	21	37	37	CD
cord-259738-yuqc6dk0	65	22	•	•	NN
cord-259738-yuqc6dk0	65	23	C	c	NN
cord-259738-yuqc6dk0	65	24	with	with	IN
cord-259738-yuqc6dk0	65	25	the	the	DT
cord-259738-yuqc6dk0	65	26	secondary	secondary	JJ
cord-259738-yuqc6dk0	65	27	FITC	FITC	NNP
cord-259738-yuqc6dk0	65	28	-	-	HYPH
cord-259738-yuqc6dk0	65	29	conjugated	conjugate	VBN
cord-259738-yuqc6dk0	65	30	goat	goat	NN
cord-259738-yuqc6dk0	65	31	-	-	HYPH
cord-259738-yuqc6dk0	65	32	anti	anti	JJ
cord-259738-yuqc6dk0	65	33	-	-	JJ
cord-259738-yuqc6dk0	65	34	rabbit	rabbit	JJ
cord-259738-yuqc6dk0	65	35	IgG	IgG	NNP
cord-259738-yuqc6dk0	65	36	antibody	antibody	NN
cord-259738-yuqc6dk0	65	37	that	that	WDT
cord-259738-yuqc6dk0	65	38	included	include	VBD
cord-259738-yuqc6dk0	65	39	0.05	0.05	CD
cord-259738-yuqc6dk0	65	40	%	%	NN
cord-259738-yuqc6dk0	65	41	azovan	azovan	NNP
cord-259738-yuqc6dk0	65	42	blue	blue	NNP
cord-259738-yuqc6dk0	65	43	(	(	-LRB-
cord-259738-yuqc6dk0	65	44	purpose	purpose	NN
cord-259738-yuqc6dk0	65	45	of	of	IN
cord-259738-yuqc6dk0	65	46	using	use	VBG
cord-259738-yuqc6dk0	65	47	azovan	azovan	NNP
cord-259738-yuqc6dk0	65	48	blue	blue	NNP
cord-259738-yuqc6dk0	65	49	was	be	VBD
cord-259738-yuqc6dk0	65	50	to	to	TO
cord-259738-yuqc6dk0	65	51	identify	identify	VB
cord-259738-yuqc6dk0	65	52	the	the	DT
cord-259738-yuqc6dk0	65	53	positive	positive	JJ
cord-259738-yuqc6dk0	65	54	Vero	Vero	NNP
cord-259738-yuqc6dk0	65	55	cells	cell	NNS
cord-259738-yuqc6dk0	65	56	and	and	CC
cord-259738-yuqc6dk0	65	57	negative	negative	JJ
cord-259738-yuqc6dk0	65	58	Vero	Vero	NNP
cord-259738-yuqc6dk0	65	59	cells	cell	NNS
cord-259738-yuqc6dk0	65	60	)	)	-RRB-
cord-259738-yuqc6dk0	65	61	.	.	.
cord-259738-yuqc6dk0	66	1	The	the	DT
cord-259738-yuqc6dk0	66	2	positive	positive	JJ
cord-259738-yuqc6dk0	66	3	Vero	Vero	NNP
cord-259738-yuqc6dk0	66	4	cells	cell	NNS
cord-259738-yuqc6dk0	66	5	expressing	express	VBG
cord-259738-yuqc6dk0	66	6	N	n	CD
cord-259738-yuqc6dk0	66	7	protein	protein	NN
cord-259738-yuqc6dk0	66	8	were	be	VBD
cord-259738-yuqc6dk0	66	9	stained	stain	VBN
cord-259738-yuqc6dk0	66	10	green	green	JJ
cord-259738-yuqc6dk0	66	11	;	;	:
cord-259738-yuqc6dk0	66	12	the	the	DT
cord-259738-yuqc6dk0	66	13	negative	negative	JJ
cord-259738-yuqc6dk0	66	14	Vero	Vero	NNP
cord-259738-yuqc6dk0	66	15	cells	cell	NNS
cord-259738-yuqc6dk0	66	16	were	be	VBD
cord-259738-yuqc6dk0	66	17	stained	stain	VBN
cord-259738-yuqc6dk0	66	18	red	red	JJ
cord-259738-yuqc6dk0	66	19	.	.	.
cord-259738-yuqc6dk0	67	1	The	the	DT
cord-259738-yuqc6dk0	67	2	cells	cell	NNS
cord-259738-yuqc6dk0	67	3	were	be	VBD
cord-259738-yuqc6dk0	67	4	washed	wash	VBN
cord-259738-yuqc6dk0	67	5	twice	twice	RB
cord-259738-yuqc6dk0	67	6	with	with	IN
cord-259738-yuqc6dk0	67	7	PBS	PBS	NNP
cord-259738-yuqc6dk0	67	8	and	and	CC
cord-259738-yuqc6dk0	67	9	analyzed	analyze	VBD
cord-259738-yuqc6dk0	67	10	the	the	DT
cord-259738-yuqc6dk0	67	11	expression	expression	NN
cord-259738-yuqc6dk0	67	12	of	of	IN
cord-259738-yuqc6dk0	67	13	the	the	DT
cord-259738-yuqc6dk0	67	14	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	67	15	plasmid	plasmid	NN
cord-259738-yuqc6dk0	67	16	by	by	IN
cord-259738-yuqc6dk0	67	17	fluorescence	fluorescence	NN
cord-259738-yuqc6dk0	67	18	microscopy	microscopy	NNS
cord-259738-yuqc6dk0	67	19	.	.	.
cord-259738-yuqc6dk0	68	1	The	the	DT
cord-259738-yuqc6dk0	68	2	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	68	3	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	68	4	-	-	HYPH
cord-259738-yuqc6dk0	68	5	N	N	NNP
cord-259738-yuqc6dk0	68	6	/	/	SYM
cord-259738-yuqc6dk0	68	7	IL2	IL2	NNP
cord-259738-yuqc6dk0	68	8	,	,	,
cord-259738-yuqc6dk0	68	9	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	68	10	-	-	HYPH
cord-259738-yuqc6dk0	68	11	N	N	NNP
cord-259738-yuqc6dk0	68	12	,	,	,
cord-259738-yuqc6dk0	68	13	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	68	14	were	be	VBD
cord-259738-yuqc6dk0	68	15	amplified	amplify	VBN
cord-259738-yuqc6dk0	68	16	in	in	IN
cord-259738-yuqc6dk0	68	17	Escherichia	Escherichia	NNP
cord-259738-yuqc6dk0	68	18	coli	coli	NNS
cord-259738-yuqc6dk0	68	19	JM	JM	NNP
cord-259738-yuqc6dk0	68	20	109	109	CD
cord-259738-yuqc6dk0	68	21	and	and	CC
cord-259738-yuqc6dk0	68	22	extracted	extract	VBD
cord-259738-yuqc6dk0	68	23	using	use	VBG
cord-259738-yuqc6dk0	68	24	the	the	DT
cord-259738-yuqc6dk0	68	25	alkaline	alkaline	JJ
cord-259738-yuqc6dk0	68	26	lysis	lysis	NN
cord-259738-yuqc6dk0	68	27	method	method	NN
cord-259738-yuqc6dk0	68	28	as	as	IN
cord-259738-yuqc6dk0	68	29	described	describe	VBN
cord-259738-yuqc6dk0	68	30	previously	previously	RB
cord-259738-yuqc6dk0	68	31	(	(	-LRB-
cord-259738-yuqc6dk0	68	32	Sambrook	Sambrook	NNP
cord-259738-yuqc6dk0	68	33	et	et	NNP
cord-259738-yuqc6dk0	68	34	al	al	NNP
cord-259738-yuqc6dk0	68	35	.	.	NNP
cord-259738-yuqc6dk0	68	36	,	,	,
cord-259738-yuqc6dk0	68	37	1989	1989	CD
cord-259738-yuqc6dk0	68	38	)	)	-RRB-
cord-259738-yuqc6dk0	68	39	.	.	.
cord-259738-yuqc6dk0	69	1	After	after	IN
cord-259738-yuqc6dk0	69	2	purification	purification	NN
cord-259738-yuqc6dk0	69	3	by	by	IN
cord-259738-yuqc6dk0	69	4	PEG8000	PEG8000	NNP
cord-259738-yuqc6dk0	69	5	precipitation	precipitation	NN
cord-259738-yuqc6dk0	69	6	,	,	,
cord-259738-yuqc6dk0	69	7	the	the	DT
cord-259738-yuqc6dk0	69	8	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	69	9	were	be	VBD
cord-259738-yuqc6dk0	69	10	resuspended	resuspend	VBN
cord-259738-yuqc6dk0	69	11	in	in	IN
cord-259738-yuqc6dk0	69	12	phosphate	phosphate	NN
cord-259738-yuqc6dk0	69	13	-	-	HYPH
cord-259738-yuqc6dk0	69	14	buffered	buffer	VBN
cord-259738-yuqc6dk0	69	15	saline	saline	NN
cord-259738-yuqc6dk0	69	16	(	(	-LRB-
cord-259738-yuqc6dk0	69	17	PBS	PBS	NNP
cord-259738-yuqc6dk0	69	18	,	,	,
cord-259738-yuqc6dk0	69	19	pH	pH	NNP
cord-259738-yuqc6dk0	69	20	7.2	7.2	CD
cord-259738-yuqc6dk0	69	21	)	)	-RRB-
cord-259738-yuqc6dk0	69	22	and	and	CC
cord-259738-yuqc6dk0	69	23	kept	keep	VBD
cord-259738-yuqc6dk0	69	24	at	at	IN
cord-259738-yuqc6dk0	69	25	−20	−20	NNP
cord-259738-yuqc6dk0	69	26	•	•	NNP
cord-259738-yuqc6dk0	69	27	C	C	NNP
cord-259738-yuqc6dk0	69	28	until	until	IN
cord-259738-yuqc6dk0	69	29	used	use	VBN
cord-259738-yuqc6dk0	69	30	for	for	IN
cord-259738-yuqc6dk0	69	31	immunization	immunization	NN
cord-259738-yuqc6dk0	69	32	.	.	.
cord-259738-yuqc6dk0	70	1	For	for	IN
cord-259738-yuqc6dk0	70	2	vaccination	vaccination	NN
cord-259738-yuqc6dk0	70	3	,	,	,
cord-259738-yuqc6dk0	70	4	the	the	DT
cord-259738-yuqc6dk0	70	5	chickens	chicken	NNS
cord-259738-yuqc6dk0	70	6	were	be	VBD
cord-259738-yuqc6dk0	70	7	randomly	randomly	RB
cord-259738-yuqc6dk0	70	8	divided	divide	VBN
cord-259738-yuqc6dk0	70	9	into	into	IN
cord-259738-yuqc6dk0	70	10	four	four	CD
cord-259738-yuqc6dk0	70	11	groups	group	NNS
cord-259738-yuqc6dk0	70	12	(	(	-LRB-
cord-259738-yuqc6dk0	70	13	n	n	NN
cord-259738-yuqc6dk0	70	14	=	=	SYM
cord-259738-yuqc6dk0	70	15	20	20	CD
cord-259738-yuqc6dk0	70	16	each	each	DT
cord-259738-yuqc6dk0	70	17	)	)	-RRB-
cord-259738-yuqc6dk0	70	18	.	.	.
cord-259738-yuqc6dk0	71	1	The	the	DT
cord-259738-yuqc6dk0	71	2	7-day	7-day	JJ
cord-259738-yuqc6dk0	71	3	-	-	HYPH
cord-259738-yuqc6dk0	71	4	old	old	JJ
cord-259738-yuqc6dk0	71	5	chickens	chicken	NNS
cord-259738-yuqc6dk0	71	6	were	be	VBD
cord-259738-yuqc6dk0	71	7	injected	inject	VBN
cord-259738-yuqc6dk0	71	8	intramuscularly	intramuscularly	RB
cord-259738-yuqc6dk0	71	9	into	into	IN
cord-259738-yuqc6dk0	71	10	the	the	DT
cord-259738-yuqc6dk0	71	11	quadriceps	quadriceps	NN
cord-259738-yuqc6dk0	71	12	muscle	muscle	NN
cord-259738-yuqc6dk0	71	13	with	with	IN
cord-259738-yuqc6dk0	71	14	150	150	CD
cord-259738-yuqc6dk0	71	15	g	g	NN
cord-259738-yuqc6dk0	71	16	of	of	IN
cord-259738-yuqc6dk0	71	17	plasmid	plasmid	NN
cord-259738-yuqc6dk0	71	18	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	71	19	-	-	HYPH
cord-259738-yuqc6dk0	71	20	N	N	NNP
cord-259738-yuqc6dk0	71	21	/	/	SYM
cord-259738-yuqc6dk0	71	22	IL2	IL2	NNP
cord-259738-yuqc6dk0	71	23	(	(	-LRB-
cord-259738-yuqc6dk0	71	24	group	group	NN
cord-259738-yuqc6dk0	71	25	1	1	CD
cord-259738-yuqc6dk0	71	26	)	)	-RRB-
cord-259738-yuqc6dk0	71	27	.	.	.
cord-259738-yuqc6dk0	72	1	A	a	DT
cord-259738-yuqc6dk0	72	2	second	second	JJ
cord-259738-yuqc6dk0	72	3	group	group	NN
cord-259738-yuqc6dk0	72	4	was	be	VBD
cord-259738-yuqc6dk0	72	5	given	give	VBN
cord-259738-yuqc6dk0	72	6	150	150	CD
cord-259738-yuqc6dk0	72	7	g	g	NN
cord-259738-yuqc6dk0	72	8	of	of	IN
cord-259738-yuqc6dk0	72	9	plasmid	plasmid	NN
cord-259738-yuqc6dk0	72	10	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	72	11	-	-	HYPH
cord-259738-yuqc6dk0	72	12	N	N	NNP
cord-259738-yuqc6dk0	72	13	(	(	-LRB-
cord-259738-yuqc6dk0	72	14	group	group	NN
cord-259738-yuqc6dk0	72	15	2	2	CD
cord-259738-yuqc6dk0	72	16	)	)	-RRB-
cord-259738-yuqc6dk0	72	17	.	.	.
cord-259738-yuqc6dk0	73	1	Other	other	JJ
cord-259738-yuqc6dk0	73	2	groups	group	NNS
cord-259738-yuqc6dk0	73	3	included	include	VBD
cord-259738-yuqc6dk0	73	4	chickens	chicken	NNS
cord-259738-yuqc6dk0	73	5	administered	administer	VBN
cord-259738-yuqc6dk0	73	6	with	with	IN
cord-259738-yuqc6dk0	73	7	150	150	CD
cord-259738-yuqc6dk0	73	8	g	g	NN
cord-259738-yuqc6dk0	73	9	of	of	IN
cord-259738-yuqc6dk0	73	10	empty	empty	JJ
cord-259738-yuqc6dk0	73	11	vector	vector	NN
cord-259738-yuqc6dk0	73	12	pIRES	pires	NN
cord-259738-yuqc6dk0	73	13	(	(	-LRB-
cord-259738-yuqc6dk0	73	14	group	group	NN
cord-259738-yuqc6dk0	73	15	3	3	CD
cord-259738-yuqc6dk0	73	16	)	)	-RRB-
cord-259738-yuqc6dk0	73	17	,	,	,
cord-259738-yuqc6dk0	73	18	and	and	CC
cord-259738-yuqc6dk0	73	19	chickens	chicken	NNS
cord-259738-yuqc6dk0	73	20	injected	inject	VBN
cord-259738-yuqc6dk0	73	21	with	with	IN
cord-259738-yuqc6dk0	73	22	0.5	0.5	CD
cord-259738-yuqc6dk0	73	23	ml	ml	NN
cord-259738-yuqc6dk0	74	1	PBS	PBS	NNP
cord-259738-yuqc6dk0	74	2	only	only	RB
cord-259738-yuqc6dk0	74	3	(	(	-LRB-
cord-259738-yuqc6dk0	74	4	group	group	NN
cord-259738-yuqc6dk0	74	5	4	4	CD
cord-259738-yuqc6dk0	74	6	)	)	-RRB-
cord-259738-yuqc6dk0	74	7	.	.	.
cord-259738-yuqc6dk0	75	1	All	all	DT
cord-259738-yuqc6dk0	75	2	groups	group	NNS
cord-259738-yuqc6dk0	75	3	were	be	VBD
cord-259738-yuqc6dk0	75	4	boosted	boost	VBN
cord-259738-yuqc6dk0	75	5	with	with	IN
cord-259738-yuqc6dk0	75	6	an	an	DT
cord-259738-yuqc6dk0	75	7	equivalent	equivalent	JJ
cord-259738-yuqc6dk0	75	8	dose	dose	NN
cord-259738-yuqc6dk0	75	9	at	at	IN
cord-259738-yuqc6dk0	75	10	21	21	CD
cord-259738-yuqc6dk0	75	11	days	day	NNS
cord-259738-yuqc6dk0	75	12	after	after	IN
cord-259738-yuqc6dk0	75	13	the	the	DT
cord-259738-yuqc6dk0	75	14	initial	initial	JJ
cord-259738-yuqc6dk0	75	15	inoculation	inoculation	NN
cord-259738-yuqc6dk0	75	16	.	.	.
cord-259738-yuqc6dk0	76	1	Pre	pre	JJ
cord-259738-yuqc6dk0	76	2	-	-	JJ
cord-259738-yuqc6dk0	76	3	vaccination	vaccination	NN
cord-259738-yuqc6dk0	76	4	sera	sera	NNP
cord-259738-yuqc6dk0	76	5	were	be	VBD
cord-259738-yuqc6dk0	76	6	collected	collect	VBN
cord-259738-yuqc6dk0	76	7	from	from	IN
cord-259738-yuqc6dk0	76	8	all	all	DT
cord-259738-yuqc6dk0	76	9	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	76	10	chickens	chicken	NNS
cord-259738-yuqc6dk0	76	11	.	.	.
cord-259738-yuqc6dk0	77	1	Blood	blood	NN
cord-259738-yuqc6dk0	77	2	was	be	VBD
cord-259738-yuqc6dk0	77	3	also	also	RB
cord-259738-yuqc6dk0	77	4	collected	collect	VBN
cord-259738-yuqc6dk0	77	5	before	before	IN
cord-259738-yuqc6dk0	77	6	booster	booster	NN
cord-259738-yuqc6dk0	77	7	vaccination	vaccination	NN
cord-259738-yuqc6dk0	77	8	as	as	RB
cord-259738-yuqc6dk0	77	9	well	well	RB
cord-259738-yuqc6dk0	77	10	as	as	IN
cord-259738-yuqc6dk0	77	11	before	before	IN
cord-259738-yuqc6dk0	77	12	challenge	challenge	NN
cord-259738-yuqc6dk0	77	13	.	.	.
cord-259738-yuqc6dk0	78	1	Sera	Sera	NNP
cord-259738-yuqc6dk0	78	2	were	be	VBD
cord-259738-yuqc6dk0	78	3	stored	store	VBN
cord-259738-yuqc6dk0	78	4	at	at	IN
cord-259738-yuqc6dk0	78	5	−20	−20	NNP
cord-259738-yuqc6dk0	78	6	•	•	NNP
cord-259738-yuqc6dk0	78	7	C	C	NNP
cord-259738-yuqc6dk0	78	8	for	for	IN
cord-259738-yuqc6dk0	78	9	serologic	serologic	NNP
cord-259738-yuqc6dk0	78	10	analysis	analysis	NN
cord-259738-yuqc6dk0	78	11	.	.	.
cord-259738-yuqc6dk0	79	1	Total	total	JJ
cord-259738-yuqc6dk0	79	2	serum	serum	NN
cord-259738-yuqc6dk0	79	3	immunoglobulin	immunoglobulin	NN
cord-259738-yuqc6dk0	79	4	G	G	NNP
cord-259738-yuqc6dk0	79	5	(	(	-LRB-
cord-259738-yuqc6dk0	79	6	IgG	IgG	NNP
cord-259738-yuqc6dk0	79	7	)	)	-RRB-
cord-259738-yuqc6dk0	79	8	specific	specific	JJ
cord-259738-yuqc6dk0	79	9	for	for	IN
cord-259738-yuqc6dk0	79	10	IBV	IBV	NNP
cord-259738-yuqc6dk0	79	11	was	be	VBD
cord-259738-yuqc6dk0	79	12	measured	measure	VBN
cord-259738-yuqc6dk0	79	13	by	by	IN
cord-259738-yuqc6dk0	79	14	indirect	indirect	JJ
cord-259738-yuqc6dk0	79	15	enzyme	enzyme	NN
cord-259738-yuqc6dk0	79	16	-	-	HYPH
cord-259738-yuqc6dk0	79	17	linked	link	VBN
cord-259738-yuqc6dk0	79	18	immunosorbent	immunosorbent	JJ
cord-259738-yuqc6dk0	79	19	assay	assay	NN
cord-259738-yuqc6dk0	79	20	(	(	-LRB-
cord-259738-yuqc6dk0	79	21	ELISA	ELISA	NNP
cord-259738-yuqc6dk0	79	22	)	)	-RRB-
cord-259738-yuqc6dk0	79	23	as	as	IN
cord-259738-yuqc6dk0	79	24	described	describe	VBN
cord-259738-yuqc6dk0	79	25	previously	previously	RB
cord-259738-yuqc6dk0	79	26	,	,	,
cord-259738-yuqc6dk0	79	27	with	with	IN
cord-259738-yuqc6dk0	79	28	brief	brief	JJ
cord-259738-yuqc6dk0	79	29	modifications	modification	NNS
cord-259738-yuqc6dk0	79	30	:	:	:
cord-259738-yuqc6dk0	79	31	ELISA	ELISA	NNP
cord-259738-yuqc6dk0	79	32	plates	plate	NNS
cord-259738-yuqc6dk0	79	33	were	be	VBD
cord-259738-yuqc6dk0	79	34	coated	coat	VBN
cord-259738-yuqc6dk0	79	35	with	with	IN
cord-259738-yuqc6dk0	79	36	IBV	IBV	NNP
cord-259738-yuqc6dk0	79	37	lysate	lysate	NN
cord-259738-yuqc6dk0	79	38	at	at	IN
cord-259738-yuqc6dk0	79	39	5	5	CD
cord-259738-yuqc6dk0	79	40	g	g	NN
cord-259738-yuqc6dk0	79	41	/	/	SYM
cord-259738-yuqc6dk0	79	42	ml	ml	NN
cord-259738-yuqc6dk0	79	43	in	in	IN
cord-259738-yuqc6dk0	79	44	carbonate	carbonate	NN
cord-259738-yuqc6dk0	79	45	buffer	buffer	NN
cord-259738-yuqc6dk0	79	46	,	,	,
cord-259738-yuqc6dk0	79	47	PH	PH	NNP
cord-259738-yuqc6dk0	79	48	9.6	9.6	CD
cord-259738-yuqc6dk0	79	49	,	,	,
cord-259738-yuqc6dk0	79	50	for	for	IN
cord-259738-yuqc6dk0	79	51	overnight	overnight	RB
cord-259738-yuqc6dk0	79	52	at	at	IN
cord-259738-yuqc6dk0	79	53	4	4	CD
cord-259738-yuqc6dk0	80	1	•	•	NNP
cord-259738-yuqc6dk0	80	2	C	C	NNP
cord-259738-yuqc6dk0	80	3	and	and	CC
cord-259738-yuqc6dk0	80	4	blocked	block	VBD
cord-259738-yuqc6dk0	80	5	with	with	IN
cord-259738-yuqc6dk0	80	6	10	10	CD
cord-259738-yuqc6dk0	80	7	%	%	NN
cord-259738-yuqc6dk0	80	8	non	non	JJ
cord-259738-yuqc6dk0	80	9	-	-	JJ
cord-259738-yuqc6dk0	80	10	fat	fat	JJ
cord-259738-yuqc6dk0	80	11	dried	dry	VBN
cord-259738-yuqc6dk0	80	12	milk	milk	NN
cord-259738-yuqc6dk0	80	13	in	in	IN
cord-259738-yuqc6dk0	80	14	PBS	PBS	NNP
cord-259738-yuqc6dk0	80	15	at	at	IN
cord-259738-yuqc6dk0	80	16	37	37	CD
cord-259738-yuqc6dk0	81	1	•	•	NNP
cord-259738-yuqc6dk0	81	2	C	C	NNP
cord-259738-yuqc6dk0	81	3	for	for	IN
cord-259738-yuqc6dk0	81	4	3	3	CD
cord-259738-yuqc6dk0	81	5	h.	h.	NN
cord-259738-yuqc6dk0	81	6	Serum	serum	NN
cord-259738-yuqc6dk0	81	7	samples	sample	NNS
cord-259738-yuqc6dk0	81	8	were	be	VBD
cord-259738-yuqc6dk0	81	9	tested	test	VBN
cord-259738-yuqc6dk0	81	10	in	in	IN
cord-259738-yuqc6dk0	81	11	1:20	1:20	CD
cord-259738-yuqc6dk0	81	12	dilution	dilution	NN
cord-259738-yuqc6dk0	81	13	in	in	IN
cord-259738-yuqc6dk0	81	14	10	10	CD
cord-259738-yuqc6dk0	81	15	%	%	NN
cord-259738-yuqc6dk0	81	16	dried	dry	VBN
cord-259738-yuqc6dk0	81	17	milk	milk	NN
cord-259738-yuqc6dk0	81	18	in	in	IN
cord-259738-yuqc6dk0	81	19	PBST	PBST	NNP
cord-259738-yuqc6dk0	81	20	.	.	.
cord-259738-yuqc6dk0	82	1	IgG	IgG	NNP
cord-259738-yuqc6dk0	82	2	against	against	IN
cord-259738-yuqc6dk0	82	3	IBV	IBV	NNP
cord-259738-yuqc6dk0	82	4	was	be	VBD
cord-259738-yuqc6dk0	82	5	revealed	reveal	VBN
cord-259738-yuqc6dk0	82	6	with	with	IN
cord-259738-yuqc6dk0	82	7	horseradish	horseradish	NN
cord-259738-yuqc6dk0	82	8	peroxidaselabeled	peroxidaselabeled	NNP
cord-259738-yuqc6dk0	82	9	goat	goat	NN
cord-259738-yuqc6dk0	82	10	-	-	HYPH
cord-259738-yuqc6dk0	82	11	anti	anti	JJ
cord-259738-yuqc6dk0	82	12	-	-	JJ
cord-259738-yuqc6dk0	82	13	chicken	chicken	JJ
cord-259738-yuqc6dk0	82	14	conjugate	conjugate	NN
cord-259738-yuqc6dk0	82	15	diluted	dilute	VBN
cord-259738-yuqc6dk0	82	16	1:2000	1:2000	CD
cord-259738-yuqc6dk0	82	17	in	in	IN
cord-259738-yuqc6dk0	82	18	PBST	PBST	NNP
cord-259738-yuqc6dk0	82	19	.	.	.
cord-259738-yuqc6dk0	83	1	The	the	DT
cord-259738-yuqc6dk0	83	2	substrate	substrate	NN
cord-259738-yuqc6dk0	83	3	solution	solution	NN
cord-259738-yuqc6dk0	83	4	used	use	VBN
cord-259738-yuqc6dk0	83	5	was	be	VBD
cord-259738-yuqc6dk0	83	6	TMB	TMB	NNP
cord-259738-yuqc6dk0	83	7	microwell	microwell	NNP
cord-259738-yuqc6dk0	83	8	peroxidase	peroxidase	NN
cord-259738-yuqc6dk0	83	9	.	.	.
cord-259738-yuqc6dk0	84	1	After	after	IN
cord-259738-yuqc6dk0	84	2	20	20	CD
cord-259738-yuqc6dk0	84	3	min	min	NN
cord-259738-yuqc6dk0	84	4	of	of	IN
cord-259738-yuqc6dk0	84	5	incubation	incubation	NN
cord-259738-yuqc6dk0	84	6	in	in	IN
cord-259738-yuqc6dk0	84	7	the	the	DT
cord-259738-yuqc6dk0	84	8	dark	dark	NN
cord-259738-yuqc6dk0	84	9	,	,	,
cord-259738-yuqc6dk0	84	10	the	the	DT
cord-259738-yuqc6dk0	84	11	reaction	reaction	NN
cord-259738-yuqc6dk0	84	12	was	be	VBD
cord-259738-yuqc6dk0	84	13	stopped	stop	VBN
cord-259738-yuqc6dk0	84	14	by	by	IN
cord-259738-yuqc6dk0	84	15	the	the	DT
cord-259738-yuqc6dk0	84	16	addition	addition	NN
cord-259738-yuqc6dk0	84	17	of	of	IN
cord-259738-yuqc6dk0	84	18	100	100	CD
cord-259738-yuqc6dk0	84	19	l	l	NN
cord-259738-yuqc6dk0	84	20	of	of	IN
cord-259738-yuqc6dk0	84	21	2	2	CD
cord-259738-yuqc6dk0	84	22	M	M	NNP
cord-259738-yuqc6dk0	84	23	H	H	NNP
cord-259738-yuqc6dk0	84	24	2	2	CD
cord-259738-yuqc6dk0	84	25	SO	so	RB
cord-259738-yuqc6dk0	84	26	4	4	CD
cord-259738-yuqc6dk0	84	27	,	,	,
cord-259738-yuqc6dk0	84	28	and	and	CC
cord-259738-yuqc6dk0	84	29	the	the	DT
cord-259738-yuqc6dk0	84	30	optical	optical	JJ
cord-259738-yuqc6dk0	84	31	density	density	NN
cord-259738-yuqc6dk0	84	32	at	at	IN
cord-259738-yuqc6dk0	84	33	450	450	CD
cord-259738-yuqc6dk0	84	34	nm	nm	NN
cord-259738-yuqc6dk0	84	35	was	be	VBD
cord-259738-yuqc6dk0	84	36	measured	measure	VBN
cord-259738-yuqc6dk0	84	37	in	in	IN
cord-259738-yuqc6dk0	84	38	an	an	DT
cord-259738-yuqc6dk0	84	39	ELISA	ELISA	NNP
cord-259738-yuqc6dk0	84	40	microplate	microplate	NN
cord-259738-yuqc6dk0	84	41	reader	reader	NN
cord-259738-yuqc6dk0	84	42	.	.	.
cord-259738-yuqc6dk0	85	1	Sera	Sera	NNP
cord-259738-yuqc6dk0	85	2	were	be	VBD
cord-259738-yuqc6dk0	85	3	run	run	VBN
cord-259738-yuqc6dk0	85	4	in	in	IN
cord-259738-yuqc6dk0	85	5	duplicate	duplicate	NN
cord-259738-yuqc6dk0	85	6	.	.	.
cord-259738-yuqc6dk0	86	1	Negative	negative	JJ
cord-259738-yuqc6dk0	86	2	and	and	CC
cord-259738-yuqc6dk0	86	3	positive	positive	JJ
cord-259738-yuqc6dk0	86	4	control	control	NN
cord-259738-yuqc6dk0	86	5	sera	sera	NNP
cord-259738-yuqc6dk0	86	6	were	be	VBD
cord-259738-yuqc6dk0	86	7	included	include	VBN
cord-259738-yuqc6dk0	86	8	in	in	IN
cord-259738-yuqc6dk0	86	9	each	each	DT
cord-259738-yuqc6dk0	86	10	assay	assay	NN
cord-259738-yuqc6dk0	86	11	.	.	.
cord-259738-yuqc6dk0	87	1	Total	total	JJ
cord-259738-yuqc6dk0	87	2	serum	serum	NN
cord-259738-yuqc6dk0	87	3	immunoglobulin	immunoglobulin	NN
cord-259738-yuqc6dk0	87	4	G	G	NNP
cord-259738-yuqc6dk0	87	5	(	(	-LRB-
cord-259738-yuqc6dk0	87	6	IgG	IgG	NNP
cord-259738-yuqc6dk0	87	7	)	)	-RRB-
cord-259738-yuqc6dk0	87	8	specific	specific	JJ
cord-259738-yuqc6dk0	87	9	for	for	IN
cord-259738-yuqc6dk0	87	10	IBV	IBV	NNP
cord-259738-yuqc6dk0	87	11	are	be	VBP
cord-259738-yuqc6dk0	87	12	represented	represent	VBN
cord-259738-yuqc6dk0	87	13	as	as	IN
cord-259738-yuqc6dk0	87	14	the	the	DT
cord-259738-yuqc6dk0	87	15	optical	optical	JJ
cord-259738-yuqc6dk0	87	16	density	density	NN
cord-259738-yuqc6dk0	87	17	.	.	.
cord-259738-yuqc6dk0	88	1	Peripheral	peripheral	JJ
cord-259738-yuqc6dk0	88	2	blood	blood	NN
cord-259738-yuqc6dk0	88	3	samples	sample	NNS
cord-259738-yuqc6dk0	88	4	from	from	IN
cord-259738-yuqc6dk0	88	5	immunized	immunize	VBN
cord-259738-yuqc6dk0	88	6	chicken	chicken	NN
cord-259738-yuqc6dk0	88	7	were	be	VBD
cord-259738-yuqc6dk0	88	8	collected	collect	VBN
cord-259738-yuqc6dk0	88	9	from	from	IN
cord-259738-yuqc6dk0	88	10	the	the	DT
cord-259738-yuqc6dk0	88	11	jugular	jugular	NN
cord-259738-yuqc6dk0	88	12	vein	vein	NN
cord-259738-yuqc6dk0	88	13	in	in	IN
cord-259738-yuqc6dk0	88	14	2.5	2.5	CD
cord-259738-yuqc6dk0	88	15	ml	ml	NNS
cord-259738-yuqc6dk0	88	16	syringes	syrinx	NNS
cord-259738-yuqc6dk0	88	17	preloaded	preloade	VBN
cord-259738-yuqc6dk0	88	18	with	with	IN
cord-259738-yuqc6dk0	88	19	0.2	0.2	CD
cord-259738-yuqc6dk0	88	20	ml	ml	NNS
cord-259738-yuqc6dk0	88	21	of	of	IN
cord-259738-yuqc6dk0	88	22	sodium	sodium	NN
cord-259738-yuqc6dk0	88	23	heparin	heparin	JJ
cord-259738-yuqc6dk0	88	24	to	to	TO
cord-259738-yuqc6dk0	88	25	prevent	prevent	VB
cord-259738-yuqc6dk0	88	26	clotting	clotting	NN
cord-259738-yuqc6dk0	88	27	on	on	IN
cord-259738-yuqc6dk0	88	28	day	day	NN
cord-259738-yuqc6dk0	88	29	7	7	CD
cord-259738-yuqc6dk0	88	30	after	after	IN
cord-259738-yuqc6dk0	88	31	the	the	DT
cord-259738-yuqc6dk0	88	32	boosting	boost	VBG
cord-259738-yuqc6dk0	88	33	vaccination	vaccination	NN
cord-259738-yuqc6dk0	88	34	.	.	.
cord-259738-yuqc6dk0	89	1	Peripheral	peripheral	JJ
cord-259738-yuqc6dk0	89	2	blood	blood	NN
cord-259738-yuqc6dk0	89	3	mononuclear	mononuclear	NN
cord-259738-yuqc6dk0	89	4	cells	cell	NNS
cord-259738-yuqc6dk0	89	5	were	be	VBD
cord-259738-yuqc6dk0	89	6	isolated	isolate	VBN
cord-259738-yuqc6dk0	89	7	from	from	IN
cord-259738-yuqc6dk0	89	8	each	each	DT
cord-259738-yuqc6dk0	89	9	blood	blood	NN
cord-259738-yuqc6dk0	89	10	sample	sample	NN
cord-259738-yuqc6dk0	89	11	by	by	IN
cord-259738-yuqc6dk0	89	12	Ficoll	Ficoll	NNP
cord-259738-yuqc6dk0	89	13	-	-	HYPH
cord-259738-yuqc6dk0	89	14	Hypaque	hypaque	JJ
cord-259738-yuqc6dk0	89	15	density	density	NN
cord-259738-yuqc6dk0	89	16	gradient	gradient	NN
cord-259738-yuqc6dk0	89	17	centrifugation	centrifugation	NN
cord-259738-yuqc6dk0	89	18	.	.	.
cord-259738-yuqc6dk0	90	1	PBMC	PBMC	NNS
cord-259738-yuqc6dk0	90	2	were	be	VBD
cord-259738-yuqc6dk0	90	3	adjusted	adjust	VBN
cord-259738-yuqc6dk0	90	4	to	to	IN
cord-259738-yuqc6dk0	90	5	1	1	CD
cord-259738-yuqc6dk0	90	6	×	×	CD
cord-259738-yuqc6dk0	90	7	10	10	CD
cord-259738-yuqc6dk0	90	8	7	7	CD
cord-259738-yuqc6dk0	90	9	cells	cell	NNS
cord-259738-yuqc6dk0	90	10	/	/	SYM
cord-259738-yuqc6dk0	90	11	ml	ml	NN
cord-259738-yuqc6dk0	90	12	.	.	.
cord-259738-yuqc6dk0	91	1	The	the	DT
cord-259738-yuqc6dk0	91	2	100	100	CD
cord-259738-yuqc6dk0	91	3	l	l	NN
cord-259738-yuqc6dk0	91	4	of	of	IN
cord-259738-yuqc6dk0	91	5	samples	sample	NNS
cord-259738-yuqc6dk0	91	6	(	(	-LRB-
cord-259738-yuqc6dk0	91	7	1	1	CD
cord-259738-yuqc6dk0	91	8	×	×	NN
cord-259738-yuqc6dk0	91	9	10	10	CD
cord-259738-yuqc6dk0	91	10	6	6	CD
cord-259738-yuqc6dk0	91	11	cells	cell	NNS
cord-259738-yuqc6dk0	91	12	)	)	-RRB-
cord-259738-yuqc6dk0	91	13	were	be	VBD
cord-259738-yuqc6dk0	91	14	incubated	incubate	VBN
cord-259738-yuqc6dk0	91	15	for	for	IN
cord-259738-yuqc6dk0	91	16	1	1	CD
cord-259738-yuqc6dk0	91	17	h	h	NN
cord-259738-yuqc6dk0	91	18	at	at	IN
cord-259738-yuqc6dk0	91	19	room	room	NN
cord-259738-yuqc6dk0	91	20	temperature	temperature	NN
cord-259738-yuqc6dk0	91	21	with	with	IN
cord-259738-yuqc6dk0	91	22	antibody	antibody	NN
cord-259738-yuqc6dk0	91	23	as	as	IN
cord-259738-yuqc6dk0	91	24	follows	follow	VBZ
cord-259738-yuqc6dk0	91	25	:	:	:
cord-259738-yuqc6dk0	91	26	mouse	mouse	NNP
cord-259738-yuqc6dk0	91	27	anti	anti	JJ
cord-259738-yuqc6dk0	91	28	-	-	JJ
cord-259738-yuqc6dk0	91	29	chicken	chicken	JJ
cord-259738-yuqc6dk0	91	30	CD4-PE	cd4-pe	NN
cord-259738-yuqc6dk0	91	31	,	,	,
cord-259738-yuqc6dk0	91	32	mouse	mouse	NN
cord-259738-yuqc6dk0	91	33	anti	anti	JJ
cord-259738-yuqc6dk0	91	34	-	-	JJ
cord-259738-yuqc6dk0	91	35	chicken	chicken	JJ
cord-259738-yuqc6dk0	91	36	CD8-FITC	CD8-FITC	NNP
cord-259738-yuqc6dk0	91	37	,	,	,
cord-259738-yuqc6dk0	91	38	mouse	mouse	NN
cord-259738-yuqc6dk0	91	39	anti	anti	JJ
cord-259738-yuqc6dk0	91	40	-	-	JJ
cord-259738-yuqc6dk0	91	41	chicken	chicken	JJ
cord-259738-yuqc6dk0	91	42	CD3-SPRD	CD3-SPRD	NNP
cord-259738-yuqc6dk0	91	43	(	(	-LRB-
cord-259738-yuqc6dk0	91	44	BD	BD	NNP
cord-259738-yuqc6dk0	91	45	Biosciences	Biosciences	NNP
cord-259738-yuqc6dk0	91	46	Pharmingen	Pharmingen	NNP
cord-259738-yuqc6dk0	91	47	)	)	-RRB-
cord-259738-yuqc6dk0	91	48	.	.	.
cord-259738-yuqc6dk0	92	1	Leukocyte	Leukocyte	NNP
cord-259738-yuqc6dk0	92	2	samples	sample	NNS
cord-259738-yuqc6dk0	92	3	were	be	VBD
cord-259738-yuqc6dk0	92	4	triply	triply	RB
cord-259738-yuqc6dk0	92	5	labeled	label	VBN
cord-259738-yuqc6dk0	92	6	with	with	IN
cord-259738-yuqc6dk0	92	7	CD3	CD3	NNP
cord-259738-yuqc6dk0	92	8	,	,	,
cord-259738-yuqc6dk0	92	9	CD4	CD4	NNP
cord-259738-yuqc6dk0	92	10	and	and	CC
cord-259738-yuqc6dk0	92	11	CD8	CD8	NNP
cord-259738-yuqc6dk0	92	12	antibodies	antibody	NNS
cord-259738-yuqc6dk0	92	13	.	.	.
cord-259738-yuqc6dk0	93	1	The	the	DT
cord-259738-yuqc6dk0	93	2	samples	sample	NNS
cord-259738-yuqc6dk0	93	3	were	be	VBD
cord-259738-yuqc6dk0	93	4	processed	process	VBN
cord-259738-yuqc6dk0	93	5	on	on	IN
cord-259738-yuqc6dk0	93	6	fluorescence	fluorescence	NN
cord-259738-yuqc6dk0	93	7	activated	activate	VBN
cord-259738-yuqc6dk0	93	8	cell	cell	NN
cord-259738-yuqc6dk0	93	9	sorter	sorter	NN
cord-259738-yuqc6dk0	93	10	.	.	.
cord-259738-yuqc6dk0	94	1	All	all	DT
cord-259738-yuqc6dk0	94	2	of	of	IN
cord-259738-yuqc6dk0	94	3	the	the	DT
cord-259738-yuqc6dk0	94	4	chickens	chicken	NNS
cord-259738-yuqc6dk0	94	5	were	be	VBD
cord-259738-yuqc6dk0	94	6	challenged	challenge	VBN
cord-259738-yuqc6dk0	94	7	with	with	IN
cord-259738-yuqc6dk0	94	8	100EID	100eid	CD
cord-259738-yuqc6dk0	94	9	50	50	CD
cord-259738-yuqc6dk0	94	10	of	of	IN
cord-259738-yuqc6dk0	94	11	the	the	DT
cord-259738-yuqc6dk0	94	12	IBV	IBV	NNP
cord-259738-yuqc6dk0	94	13	SAIBk	SAIBk	NNPS
cord-259738-yuqc6dk0	94	14	strain	strain	NN
cord-259738-yuqc6dk0	94	15	in	in	IN
cord-259738-yuqc6dk0	94	16	0.1	0.1	CD
cord-259738-yuqc6dk0	94	17	ml	ml	NNS
cord-259738-yuqc6dk0	94	18	by	by	IN
cord-259738-yuqc6dk0	94	19	the	the	DT
cord-259738-yuqc6dk0	94	20	nasal	nasal	JJ
cord-259738-yuqc6dk0	94	21	-	-	HYPH
cord-259738-yuqc6dk0	94	22	ocular	ocular	JJ
cord-259738-yuqc6dk0	94	23	route	route	NN
cord-259738-yuqc6dk0	94	24	at	at	IN
cord-259738-yuqc6dk0	94	25	15	15	CD
cord-259738-yuqc6dk0	94	26	days	day	NNS
cord-259738-yuqc6dk0	94	27	after	after	IN
cord-259738-yuqc6dk0	94	28	the	the	DT
cord-259738-yuqc6dk0	94	29	boosting	boost	VBG
cord-259738-yuqc6dk0	94	30	immunization	immunization	NN
cord-259738-yuqc6dk0	94	31	.	.	.
cord-259738-yuqc6dk0	95	1	The	the	DT
cord-259738-yuqc6dk0	95	2	challenged	challenge	VBN
cord-259738-yuqc6dk0	95	3	chickens	chicken	NNS
cord-259738-yuqc6dk0	95	4	were	be	VBD
cord-259738-yuqc6dk0	95	5	examined	examine	VBN
cord-259738-yuqc6dk0	95	6	daily	daily	RB
cord-259738-yuqc6dk0	95	7	for	for	IN
cord-259738-yuqc6dk0	95	8	signs	sign	NNS
cord-259738-yuqc6dk0	95	9	of	of	IN
cord-259738-yuqc6dk0	95	10	clinical	clinical	JJ
cord-259738-yuqc6dk0	95	11	illness	illness	NN
cord-259738-yuqc6dk0	95	12	such	such	JJ
cord-259738-yuqc6dk0	95	13	as	as	IN
cord-259738-yuqc6dk0	95	14	coughing	coughing	NN
cord-259738-yuqc6dk0	95	15	,	,	,
cord-259738-yuqc6dk0	95	16	sneezing	sneezing	NN
cord-259738-yuqc6dk0	95	17	,	,	,
cord-259738-yuqc6dk0	95	18	ataxia	ataxia	NN
cord-259738-yuqc6dk0	95	19	,	,	,
cord-259738-yuqc6dk0	95	20	dyspnea	dyspnea	NN
cord-259738-yuqc6dk0	95	21	or	or	CC
cord-259738-yuqc6dk0	95	22	death	death	NN
cord-259738-yuqc6dk0	95	23	for	for	IN
cord-259738-yuqc6dk0	95	24	2	2	CD
cord-259738-yuqc6dk0	95	25	weeks	week	NNS
cord-259738-yuqc6dk0	95	26	.	.	.
cord-259738-yuqc6dk0	96	1	Dead	dead	JJ
cord-259738-yuqc6dk0	96	2	chickens	chicken	NNS
cord-259738-yuqc6dk0	96	3	were	be	VBD
cord-259738-yuqc6dk0	96	4	necropsied	necropsie	VBN
cord-259738-yuqc6dk0	96	5	to	to	TO
cord-259738-yuqc6dk0	96	6	confirm	confirm	VB
cord-259738-yuqc6dk0	96	7	death	death	NN
cord-259738-yuqc6dk0	96	8	by	by	IN
cord-259738-yuqc6dk0	96	9	IBV	IBV	NNP
cord-259738-yuqc6dk0	96	10	infection	infection	NN
cord-259738-yuqc6dk0	96	11	.	.	.
cord-259738-yuqc6dk0	97	1	The	the	DT
cord-259738-yuqc6dk0	97	2	challenged	challenge	VBN
cord-259738-yuqc6dk0	97	3	chickens	chicken	NNS
cord-259738-yuqc6dk0	97	4	generally	generally	RB
cord-259738-yuqc6dk0	97	5	began	begin	VBD
cord-259738-yuqc6dk0	97	6	to	to	TO
cord-259738-yuqc6dk0	97	7	show	show	VB
cord-259738-yuqc6dk0	97	8	clinical	clinical	JJ
cord-259738-yuqc6dk0	97	9	signs	sign	NNS
cord-259738-yuqc6dk0	97	10	from	from	IN
cord-259738-yuqc6dk0	97	11	4	4	CD
cord-259738-yuqc6dk0	97	12	to	to	TO
cord-259738-yuqc6dk0	97	13	10	10	CD
cord-259738-yuqc6dk0	97	14	days	day	NNS
cord-259738-yuqc6dk0	97	15	after	after	IN
cord-259738-yuqc6dk0	97	16	challenge	challenge	NN
cord-259738-yuqc6dk0	97	17	.	.	.
cord-259738-yuqc6dk0	98	1	Chickens	chicken	NNS
cord-259738-yuqc6dk0	98	2	in	in	IN
cord-259738-yuqc6dk0	98	3	each	each	DT
cord-259738-yuqc6dk0	98	4	group	group	NN
cord-259738-yuqc6dk0	98	5	were	be	VBD
cord-259738-yuqc6dk0	98	6	euthanized	euthanize	VBN
cord-259738-yuqc6dk0	98	7	at	at	IN
cord-259738-yuqc6dk0	98	8	14	14	CD
cord-259738-yuqc6dk0	98	9	days	day	NNS
cord-259738-yuqc6dk0	98	10	post	post	NN
cord-259738-yuqc6dk0	98	11	-	-	NN
cord-259738-yuqc6dk0	98	12	infection	infection	NN
cord-259738-yuqc6dk0	98	13	.	.	.
cord-259738-yuqc6dk0	99	1	Necropsies	Necropsies	NNP
cord-259738-yuqc6dk0	99	2	were	be	VBD
cord-259738-yuqc6dk0	99	3	performed	perform	VBN
cord-259738-yuqc6dk0	99	4	immediately	immediately	RB
cord-259738-yuqc6dk0	99	5	postmortem	postmortem	JJ
cord-259738-yuqc6dk0	99	6	and	and	CC
cord-259738-yuqc6dk0	99	7	kidney	kidney	NN
cord-259738-yuqc6dk0	99	8	tissues	tissue	NNS
cord-259738-yuqc6dk0	99	9	were	be	VBD
cord-259738-yuqc6dk0	99	10	collected	collect	VBN
cord-259738-yuqc6dk0	99	11	for	for	IN
cord-259738-yuqc6dk0	99	12	further	further	JJ
cord-259738-yuqc6dk0	99	13	detection	detection	NN
cord-259738-yuqc6dk0	99	14	of	of	IN
cord-259738-yuqc6dk0	99	15	virus	virus	NN
cord-259738-yuqc6dk0	99	16	.	.	.
cord-259738-yuqc6dk0	99	17	 	 	_SP
cord-259738-yuqc6dk0	100	1	The	the	DT
cord-259738-yuqc6dk0	100	2	kidney	kidney	NN
cord-259738-yuqc6dk0	100	3	tissues	tissue	NNS
cord-259738-yuqc6dk0	100	4	were	be	VBD
cord-259738-yuqc6dk0	100	5	incised	incise	VBN
cord-259738-yuqc6dk0	100	6	individually	individually	RB
cord-259738-yuqc6dk0	100	7	from	from	IN
cord-259738-yuqc6dk0	100	8	either	either	CC
cord-259738-yuqc6dk0	100	9	the	the	DT
cord-259738-yuqc6dk0	100	10	dead	dead	JJ
cord-259738-yuqc6dk0	100	11	or	or	CC
cord-259738-yuqc6dk0	100	12	euthanized	euthanize	VBN
cord-259738-yuqc6dk0	100	13	chickens	chicken	NNS
cord-259738-yuqc6dk0	100	14	at	at	IN
cord-259738-yuqc6dk0	100	15	14	14	CD
cord-259738-yuqc6dk0	100	16	days	day	NNS
cord-259738-yuqc6dk0	100	17	post	post	NN
cord-259738-yuqc6dk0	100	18	-	-	JJ
cord-259738-yuqc6dk0	100	19	challenged	challenge	VBN
cord-259738-yuqc6dk0	100	20	.	.	.
cord-259738-yuqc6dk0	101	1	The	the	DT
cord-259738-yuqc6dk0	101	2	virus	virus	NN
cord-259738-yuqc6dk0	101	3	in	in	IN
cord-259738-yuqc6dk0	101	4	the	the	DT
cord-259738-yuqc6dk0	101	5	kidney	kidney	NN
cord-259738-yuqc6dk0	101	6	tissues	tissue	NNS
cord-259738-yuqc6dk0	101	7	of	of	IN
cord-259738-yuqc6dk0	101	8	the	the	DT
cord-259738-yuqc6dk0	101	9	challenged	challenge	VBN
cord-259738-yuqc6dk0	101	10	chicken	chicken	NN
cord-259738-yuqc6dk0	101	11	was	be	VBD
cord-259738-yuqc6dk0	101	12	detected	detect	VBN
cord-259738-yuqc6dk0	101	13	by	by	IN
cord-259738-yuqc6dk0	101	14	RT	RT	NNP
cord-259738-yuqc6dk0	101	15	-	-	HYPH
cord-259738-yuqc6dk0	101	16	PCR	PCR	NNP
cord-259738-yuqc6dk0	101	17	.	.	.
cord-259738-yuqc6dk0	102	1	Total	Total	NNP
cord-259738-yuqc6dk0	102	2	RNA	RNA	NNP
cord-259738-yuqc6dk0	102	3	was	be	VBD
cord-259738-yuqc6dk0	102	4	extracted	extract	VBN
cord-259738-yuqc6dk0	102	5	using	use	VBG
cord-259738-yuqc6dk0	102	6	Trizol	Trizol	NNP
cord-259738-yuqc6dk0	102	7	LS	LS	NNP
cord-259738-yuqc6dk0	102	8	reagent	reagent	NN
cord-259738-yuqc6dk0	102	9	and	and	CC
cord-259738-yuqc6dk0	102	10	subjected	subject	VBN
cord-259738-yuqc6dk0	102	11	to	to	IN
cord-259738-yuqc6dk0	102	12	RT	RT	NNP
cord-259738-yuqc6dk0	102	13	-	-	HYPH
cord-259738-yuqc6dk0	102	14	PCR	PCR	NNP
cord-259738-yuqc6dk0	102	15	using	use	VBG
cord-259738-yuqc6dk0	102	16	primers	primer	NNS
cord-259738-yuqc6dk0	102	17	directed	direct	VBN
cord-259738-yuqc6dk0	102	18	to	to	IN
cord-259738-yuqc6dk0	102	19	the	the	DT
cord-259738-yuqc6dk0	102	20	3	3	CD
cord-259738-yuqc6dk0	102	21	untranslated	untranslated	JJ
cord-259738-yuqc6dk0	102	22	region	region	NN
cord-259738-yuqc6dk0	102	23	(	(	-LRB-
cord-259738-yuqc6dk0	102	24	forward	forward	NNP
cord-259738-yuqc6dk0	102	25	primer	primer	NN
cord-259738-yuqc6dk0	102	26	:	:	:
cord-259738-yuqc6dk0	102	27	5	5	CD
cord-259738-yuqc6dk0	102	28	-GATGAGGAGAGGAACAATGC-3	-GATGAGGAGAGGAACAATGC-3	NNP
cord-259738-yuqc6dk0	102	29	;	;	:
cord-259738-yuqc6dk0	102	30	reverse	reverse	JJ
cord-259738-yuqc6dk0	102	31	primer	primer	NN
cord-259738-yuqc6dk0	102	32	:	:	:
cord-259738-yuqc6dk0	102	33	5	5	CD
cord-259738-yuqc6dk0	102	34	-TGGGCGTCCTAGTGCTGT-3	-TGGGCGTCCTAGTGCTGT-3	NNP
cord-259738-yuqc6dk0	102	35	)	)	-RRB-
cord-259738-yuqc6dk0	102	36	.	.	.
cord-259738-yuqc6dk0	103	1	Total	total	JJ
cord-259738-yuqc6dk0	103	2	protection	protection	NN
cord-259738-yuqc6dk0	103	3	was	be	VBD
cord-259738-yuqc6dk0	103	4	defined	define	VBN
cord-259738-yuqc6dk0	103	5	as	as	IN
cord-259738-yuqc6dk0	103	6	negative	negative	JJ
cord-259738-yuqc6dk0	103	7	for	for	IN
cord-259738-yuqc6dk0	103	8	the	the	DT
cord-259738-yuqc6dk0	103	9	presence	presence	NN
cord-259738-yuqc6dk0	103	10	of	of	IN
cord-259738-yuqc6dk0	103	11	virus	virus	NN
cord-259738-yuqc6dk0	103	12	in	in	IN
cord-259738-yuqc6dk0	103	13	the	the	DT
cord-259738-yuqc6dk0	103	14	kidney	kidney	NN
cord-259738-yuqc6dk0	103	15	.	.	.
cord-259738-yuqc6dk0	104	1	Data	datum	NNS
cord-259738-yuqc6dk0	104	2	were	be	VBD
cord-259738-yuqc6dk0	104	3	analyzed	analyze	VBN
cord-259738-yuqc6dk0	104	4	using	use	VBG
cord-259738-yuqc6dk0	104	5	the	the	DT
cord-259738-yuqc6dk0	104	6	one	one	CD
cord-259738-yuqc6dk0	104	7	-	-	HYPH
cord-259738-yuqc6dk0	104	8	sided	sided	JJ
cord-259738-yuqc6dk0	104	9	Student	Student	NNP
cord-259738-yuqc6dk0	104	10	's	's	POS
cord-259738-yuqc6dk0	104	11	t	t	NN
cord-259738-yuqc6dk0	104	12	test	test	NN
cord-259738-yuqc6dk0	104	13	.	.	.
cord-259738-yuqc6dk0	105	1	Differences	difference	NNS
cord-259738-yuqc6dk0	105	2	were	be	VBD
cord-259738-yuqc6dk0	105	3	considered	consider	VBN
cord-259738-yuqc6dk0	105	4	statistically	statistically	RB
cord-259738-yuqc6dk0	105	5	significant	significant	JJ
cord-259738-yuqc6dk0	105	6	with	with	IN
cord-259738-yuqc6dk0	105	7	P	p	NN
cord-259738-yuqc6dk0	105	8	<	<	XX
cord-259738-yuqc6dk0	105	9	0.05	0.05	CD
cord-259738-yuqc6dk0	105	10	.	.	.
cord-259738-yuqc6dk0	106	1	The	the	DT
cord-259738-yuqc6dk0	106	2	monocistronic	monocistronic	NN
cord-259738-yuqc6dk0	106	3	and	and	CC
cord-259738-yuqc6dk0	106	4	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	106	5	DNA	DNA	NNP
cord-259738-yuqc6dk0	106	6	vaccine	vaccine	NN
cord-259738-yuqc6dk0	106	7	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	106	8	encoding	encode	VBG
cord-259738-yuqc6dk0	106	9	IBV	IBV	NNP
cord-259738-yuqc6dk0	106	10	N	N	NNP
cord-259738-yuqc6dk0	106	11	protein	protein	NN
cord-259738-yuqc6dk0	106	12	and	and	CC
cord-259738-yuqc6dk0	106	13	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	106	14	were	be	VBD
cord-259738-yuqc6dk0	106	15	constructed	construct	VBN
cord-259738-yuqc6dk0	106	16	using	use	VBG
cord-259738-yuqc6dk0	106	17	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	106	18	plasmid	plasmid	NNP
cord-259738-yuqc6dk0	106	19	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	106	20	-	-	HYPH
cord-259738-yuqc6dk0	106	21	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	106	22	/	/	SYM
cord-259738-yuqc6dk0	106	23	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	106	24	as	as	IN
cord-259738-yuqc6dk0	106	25	shown	show	VBN
cord-259738-yuqc6dk0	106	26	in	in	IN
cord-259738-yuqc6dk0	106	27	Fig	Fig	NNP
cord-259738-yuqc6dk0	106	28	.	.	.
cord-259738-yuqc6dk0	107	1	1	1	LS
cord-259738-yuqc6dk0	107	2	.	.	.
cord-259738-yuqc6dk0	108	1	To	to	TO
cord-259738-yuqc6dk0	108	2	confirm	confirm	VB
cord-259738-yuqc6dk0	108	3	the	the	DT
cord-259738-yuqc6dk0	108	4	transcription	transcription	NN
cord-259738-yuqc6dk0	108	5	of	of	IN
cord-259738-yuqc6dk0	108	6	constructs	construct	NNS
cord-259738-yuqc6dk0	109	1	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	109	2	-	-	HYPH
cord-259738-yuqc6dk0	109	3	N	N	NNP
cord-259738-yuqc6dk0	109	4	/	/	SYM
cord-259738-yuqc6dk0	109	5	IL2	IL2	NNP
cord-259738-yuqc6dk0	109	6	,	,	,
cord-259738-yuqc6dk0	109	7	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	109	8	-	-	HYPH
cord-259738-yuqc6dk0	109	9	N	N	NNP
cord-259738-yuqc6dk0	109	10	in	in	IN
cord-259738-yuqc6dk0	109	11	a	a	DT
cord-259738-yuqc6dk0	109	12	eukaryotic	eukaryotic	JJ
cord-259738-yuqc6dk0	109	13	system	system	NN
cord-259738-yuqc6dk0	109	14	,	,	,
cord-259738-yuqc6dk0	109	15	the	the	DT
cord-259738-yuqc6dk0	109	16	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	109	17	were	be	VBD
cord-259738-yuqc6dk0	109	18	transfected	transfecte	VBN
cord-259738-yuqc6dk0	109	19	respectively	respectively	RB
cord-259738-yuqc6dk0	109	20	into	into	IN
cord-259738-yuqc6dk0	109	21	Vero	Vero	NNP
cord-259738-yuqc6dk0	109	22	cells	cell	NNS
cord-259738-yuqc6dk0	109	23	.	.	.
cord-259738-yuqc6dk0	110	1	Total	Total	NNP
cord-259738-yuqc6dk0	110	2	RNA	RNA	NNP
cord-259738-yuqc6dk0	110	3	was	be	VBD
cord-259738-yuqc6dk0	110	4	extracted	extract	VBN
cord-259738-yuqc6dk0	110	5	from	from	IN
cord-259738-yuqc6dk0	110	6	transfected	transfected	JJ
cord-259738-yuqc6dk0	110	7	cells	cell	NNS
cord-259738-yuqc6dk0	110	8	at	at	IN
cord-259738-yuqc6dk0	110	9	38	38	CD
cord-259738-yuqc6dk0	110	10	h	h	NN
cord-259738-yuqc6dk0	110	11	and	and	CC
cord-259738-yuqc6dk0	110	12	analyzed	analyze	VBN
cord-259738-yuqc6dk0	110	13	by	by	IN
cord-259738-yuqc6dk0	110	14	RT	RT	NNP
cord-259738-yuqc6dk0	110	15	-	-	HYPH
cord-259738-yuqc6dk0	110	16	PCR	PCR	NNP
cord-259738-yuqc6dk0	110	17	for	for	IN
cord-259738-yuqc6dk0	110	18	the	the	DT
cord-259738-yuqc6dk0	110	19	presence	presence	NN
cord-259738-yuqc6dk0	110	20	of	of	IN
cord-259738-yuqc6dk0	110	21	each	each	DT
cord-259738-yuqc6dk0	110	22	corresponding	correspond	VBG
cord-259738-yuqc6dk0	110	23	mRNA	mrna	NN
cord-259738-yuqc6dk0	110	24	.	.	.
cord-259738-yuqc6dk0	111	1	The	the	DT
cord-259738-yuqc6dk0	111	2	predicted	predict	VBN
cord-259738-yuqc6dk0	111	3	RT	RT	NNP
cord-259738-yuqc6dk0	111	4	-	-	HYPH
cord-259738-yuqc6dk0	111	5	PCR	PCR	NNP
cord-259738-yuqc6dk0	111	6	products	product	NNS
cord-259738-yuqc6dk0	111	7	were	be	VBD
cord-259738-yuqc6dk0	111	8	of	of	IN
cord-259738-yuqc6dk0	111	9	1.3	1.3	CD
cord-259738-yuqc6dk0	111	10	kb	kb	NNS
cord-259738-yuqc6dk0	111	11	in	in	IN
cord-259738-yuqc6dk0	111	12	size	size	NN
cord-259738-yuqc6dk0	111	13	for	for	IN
cord-259738-yuqc6dk0	111	14	N	n	CD
cord-259738-yuqc6dk0	111	15	and	and	CC
cord-259738-yuqc6dk0	111	16	0.6	0.6	CD
cord-259738-yuqc6dk0	111	17	kb	kb	NNS
cord-259738-yuqc6dk0	111	18	for	for	IN
cord-259738-yuqc6dk0	111	19	IL2	IL2	NNP
cord-259738-yuqc6dk0	111	20	,	,	,
cord-259738-yuqc6dk0	111	21	all	all	DT
cord-259738-yuqc6dk0	111	22	of	of	IN
cord-259738-yuqc6dk0	111	23	which	which	WDT
cord-259738-yuqc6dk0	111	24	were	be	VBD
cord-259738-yuqc6dk0	111	25	confirmed	confirm	VBN
cord-259738-yuqc6dk0	111	26	by	by	IN
cord-259738-yuqc6dk0	111	27	gel	gel	NN
cord-259738-yuqc6dk0	111	28	electrophoresis	electrophoresis	NN
cord-259738-yuqc6dk0	111	29	.	.	.
cord-259738-yuqc6dk0	112	1	No	no	DT
cord-259738-yuqc6dk0	112	2	specific	specific	JJ
cord-259738-yuqc6dk0	112	3	band	band	NN
cord-259738-yuqc6dk0	112	4	of	of	IN
cord-259738-yuqc6dk0	112	5	a	a	DT
cord-259738-yuqc6dk0	112	6	similar	similar	JJ
cord-259738-yuqc6dk0	112	7	size	size	NN
cord-259738-yuqc6dk0	112	8	was	be	VBD
cord-259738-yuqc6dk0	112	9	seen	see	VBN
cord-259738-yuqc6dk0	112	10	in	in	IN
cord-259738-yuqc6dk0	112	11	any	any	DT
cord-259738-yuqc6dk0	112	12	of	of	IN
cord-259738-yuqc6dk0	112	13	the	the	DT
cord-259738-yuqc6dk0	112	14	mRNA	mRNA	NNP
cord-259738-yuqc6dk0	112	15	samples	sample	NNS
cord-259738-yuqc6dk0	112	16	in	in	IN
cord-259738-yuqc6dk0	112	17	the	the	DT
cord-259738-yuqc6dk0	112	18	absence	absence	NN
cord-259738-yuqc6dk0	112	19	of	of	IN
cord-259738-yuqc6dk0	112	20	reverse	reverse	JJ
cord-259738-yuqc6dk0	112	21	transcription	transcription	NN
cord-259738-yuqc6dk0	112	22	(	(	-LRB-
cord-259738-yuqc6dk0	112	23	Fig	fig	NN
cord-259738-yuqc6dk0	112	24	.	.	NNP
cord-259738-yuqc6dk0	112	25	2	2	CD
cord-259738-yuqc6dk0	112	26	)	)	-RRB-
cord-259738-yuqc6dk0	112	27	.	.	.
cord-259738-yuqc6dk0	113	1	This	this	DT
cord-259738-yuqc6dk0	113	2	result	result	NN
cord-259738-yuqc6dk0	113	3	showed	show	VBD
cord-259738-yuqc6dk0	113	4	that	that	IN
cord-259738-yuqc6dk0	113	5	constructs	construct	NNS
cord-259738-yuqc6dk0	113	6	encoding	encode	VBG
cord-259738-yuqc6dk0	113	7	N	N	NNP
cord-259738-yuqc6dk0	113	8	and	and	CC
cord-259738-yuqc6dk0	113	9	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	113	10	gene	gene	NN
cord-259738-yuqc6dk0	113	11	can	can	MD
cord-259738-yuqc6dk0	113	12	be	be	VB
cord-259738-yuqc6dk0	113	13	transcribed	transcribe	VBN
cord-259738-yuqc6dk0	113	14	successfully	successfully	RB
cord-259738-yuqc6dk0	113	15	in	in	IN
cord-259738-yuqc6dk0	113	16	the	the	DT
cord-259738-yuqc6dk0	113	17	eukaryotic	eukaryotic	JJ
cord-259738-yuqc6dk0	113	18	system	system	NN
cord-259738-yuqc6dk0	113	19	.	.	.
cord-259738-yuqc6dk0	114	1	The	the	DT
cord-259738-yuqc6dk0	114	2	expression	expression	NN
cord-259738-yuqc6dk0	114	3	of	of	IN
cord-259738-yuqc6dk0	114	4	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	114	5	-	-	HYPH
cord-259738-yuqc6dk0	114	6	N	N	NNP
cord-259738-yuqc6dk0	114	7	/	/	SYM
cord-259738-yuqc6dk0	114	8	IL2	IL2	NNP
cord-259738-yuqc6dk0	114	9	,	,	,
cord-259738-yuqc6dk0	114	10	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	114	11	-	-	HYPH
cord-259738-yuqc6dk0	114	12	N	N	NNP
cord-259738-yuqc6dk0	114	13	was	be	VBD
cord-259738-yuqc6dk0	114	14	demonstrated	demonstrate	VBN
cord-259738-yuqc6dk0	114	15	by	by	IN
cord-259738-yuqc6dk0	114	16	indirect	indirect	JJ
cord-259738-yuqc6dk0	114	17	immunofluorescence	immunofluorescence	NN
cord-259738-yuqc6dk0	114	18	assay	assay	NN
cord-259738-yuqc6dk0	114	19	.	.	.
cord-259738-yuqc6dk0	115	1	After	after	IN
cord-259738-yuqc6dk0	115	2	transfection	transfection	NN
cord-259738-yuqc6dk0	115	3	with	with	IN
cord-259738-yuqc6dk0	115	4	lipofectamine	lipofectamine	NN
cord-259738-yuqc6dk0	115	5	,	,	,
cord-259738-yuqc6dk0	115	6	the	the	DT
cord-259738-yuqc6dk0	115	7	transfected	transfecte	VBN
cord-259738-yuqc6dk0	115	8	cells	cell	NNS
cord-259738-yuqc6dk0	115	9	displayed	display	VBD
cord-259738-yuqc6dk0	115	10	positive	positive	JJ
cord-259738-yuqc6dk0	115	11	signals	signal	NNS
cord-259738-yuqc6dk0	115	12	for	for	IN
cord-259738-yuqc6dk0	115	13	the	the	DT
cord-259738-yuqc6dk0	115	14	protein	protein	NN
cord-259738-yuqc6dk0	115	15	and	and	CC
cord-259738-yuqc6dk0	115	16	located	locate	VBN
cord-259738-yuqc6dk0	115	17	cytoplasm	cytoplasm	NN
cord-259738-yuqc6dk0	115	18	,	,	,
cord-259738-yuqc6dk0	115	19	where	where	WRB
cord-259738-yuqc6dk0	115	20	there	there	EX
cord-259738-yuqc6dk0	115	21	was	be	VBD
cord-259738-yuqc6dk0	115	22	green	green	JJ
cord-259738-yuqc6dk0	115	23	fluorescence	fluorescence	NN
cord-259738-yuqc6dk0	115	24	.	.	.
cord-259738-yuqc6dk0	116	1	Expression	expression	NN
cord-259738-yuqc6dk0	116	2	of	of	IN
cord-259738-yuqc6dk0	116	3	N	n	CD
cord-259738-yuqc6dk0	116	4	protein	protein	NN
cord-259738-yuqc6dk0	116	5	was	be	VBD
cord-259738-yuqc6dk0	116	6	not	not	RB
cord-259738-yuqc6dk0	116	7	detected	detect	VBN
cord-259738-yuqc6dk0	116	8	in	in	IN
cord-259738-yuqc6dk0	116	9	transfected	transfecte	VBN
cord-259738-yuqc6dk0	116	10	empty	empty	JJ
cord-259738-yuqc6dk0	116	11	plasmid	plasmid	NN
cord-259738-yuqc6dk0	116	12	control	control	NN
cord-259738-yuqc6dk0	116	13	cells	cell	NNS
cord-259738-yuqc6dk0	116	14	(	(	-LRB-
cord-259738-yuqc6dk0	116	15	Fig	Fig	NNP
cord-259738-yuqc6dk0	116	16	.	.	.
cord-259738-yuqc6dk0	116	17	3	3	CD
cord-259738-yuqc6dk0	116	18	)	)	-RRB-
cord-259738-yuqc6dk0	116	19	.	.	.
cord-259738-yuqc6dk0	117	1	This	this	DT
cord-259738-yuqc6dk0	117	2	result	result	NN
cord-259738-yuqc6dk0	117	3	shows	show	VBZ
cord-259738-yuqc6dk0	117	4	that	that	IN
cord-259738-yuqc6dk0	117	5	constructs	construct	VBZ
cord-259738-yuqc6dk0	117	6	encoding	encode	VBG
cord-259738-yuqc6dk0	117	7	N	n	CD
cord-259738-yuqc6dk0	117	8	protein	protein	NN
cord-259738-yuqc6dk0	117	9	can	can	MD
cord-259738-yuqc6dk0	117	10	be	be	VB
cord-259738-yuqc6dk0	117	11	successfully	successfully	RB
cord-259738-yuqc6dk0	117	12	expressed	express	VBN
cord-259738-yuqc6dk0	117	13	in	in	IN
cord-259738-yuqc6dk0	117	14	the	the	DT
cord-259738-yuqc6dk0	117	15	eukaryotic	eukaryotic	JJ
cord-259738-yuqc6dk0	117	16	system	system	NN
cord-259738-yuqc6dk0	117	17	.	.	.
cord-259738-yuqc6dk0	117	18	 	 	_SP
cord-259738-yuqc6dk0	118	1	The	the	DT
cord-259738-yuqc6dk0	118	2	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	119	1	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	119	2	-	-	HYPH
cord-259738-yuqc6dk0	119	3	N	N	NNP
cord-259738-yuqc6dk0	119	4	/	/	SYM
cord-259738-yuqc6dk0	119	5	IL2	IL2	NNP
cord-259738-yuqc6dk0	119	6	,	,	,
cord-259738-yuqc6dk0	119	7	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	119	8	-	-	HYPH
cord-259738-yuqc6dk0	119	9	N	N	NNP
cord-259738-yuqc6dk0	119	10	induced	induce	VBN
cord-259738-yuqc6dk0	119	11	detectable	detectable	JJ
cord-259738-yuqc6dk0	119	12	antibodies	antibody	NNS
cord-259738-yuqc6dk0	119	13	to	to	IN
cord-259738-yuqc6dk0	119	14	IBV	IBV	NNP
cord-259738-yuqc6dk0	119	15	Ag	Ag	NNP
cord-259738-yuqc6dk0	119	16	in	in	IN
cord-259738-yuqc6dk0	119	17	chickens	chicken	NNS
cord-259738-yuqc6dk0	119	18	one	one	CD
cord-259738-yuqc6dk0	119	19	week	week	NN
cord-259738-yuqc6dk0	119	20	after	after	IN
cord-259738-yuqc6dk0	119	21	injection	injection	NN
cord-259738-yuqc6dk0	119	22	and	and	CC
cord-259738-yuqc6dk0	119	23	the	the	DT
cord-259738-yuqc6dk0	119	24	levels	level	NNS
cord-259738-yuqc6dk0	119	25	increased	increase	VBD
cord-259738-yuqc6dk0	119	26	with	with	IN
cord-259738-yuqc6dk0	119	27	subsequent	subsequent	JJ
cord-259738-yuqc6dk0	119	28	vaccination	vaccination	NN
cord-259738-yuqc6dk0	119	29	.	.	.
cord-259738-yuqc6dk0	120	1	There	there	EX
cord-259738-yuqc6dk0	120	2	was	be	VBD
cord-259738-yuqc6dk0	120	3	no	no	DT
cord-259738-yuqc6dk0	120	4	specific	specific	JJ
cord-259738-yuqc6dk0	120	5	antibody	antibody	NN
cord-259738-yuqc6dk0	120	6	response	response	NN
cord-259738-yuqc6dk0	120	7	in	in	IN
cord-259738-yuqc6dk0	120	8	the	the	DT
cord-259738-yuqc6dk0	120	9	group	group	NN
cord-259738-yuqc6dk0	120	10	of	of	IN
cord-259738-yuqc6dk0	120	11	chickens	chicken	NNS
cord-259738-yuqc6dk0	120	12	receiving	receive	VBG
cord-259738-yuqc6dk0	120	13	PBS	PBS	NNP
cord-259738-yuqc6dk0	120	14	and	and	CC
cord-259738-yuqc6dk0	120	15	pIRES	pires	NN
cord-259738-yuqc6dk0	120	16	plasmid	plasmid	NN
cord-259738-yuqc6dk0	120	17	.	.	.
cord-259738-yuqc6dk0	121	1	There	there	EX
cord-259738-yuqc6dk0	121	2	was	be	VBD
cord-259738-yuqc6dk0	121	3	a	a	DT
cord-259738-yuqc6dk0	121	4	significant	significant	JJ
cord-259738-yuqc6dk0	121	5	difference	difference	NN
cord-259738-yuqc6dk0	121	6	in	in	IN
cord-259738-yuqc6dk0	121	7	ELISA	ELISA	NNP
cord-259738-yuqc6dk0	121	8	antibody	antibody	NN
cord-259738-yuqc6dk0	121	9	levels	level	NNS
cord-259738-yuqc6dk0	121	10	(	(	-LRB-
cord-259738-yuqc6dk0	121	11	P	p	NN
cord-259738-yuqc6dk0	121	12	<	<	XX
cord-259738-yuqc6dk0	121	13	0.05	0.05	CD
cord-259738-yuqc6dk0	121	14	)	)	-RRB-
cord-259738-yuqc6dk0	121	15	elicited	elicit	VBN
cord-259738-yuqc6dk0	121	16	by	by	IN
cord-259738-yuqc6dk0	121	17	either	either	DT
cord-259738-yuqc6dk0	121	18	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	121	19	or	or	CC
cord-259738-yuqc6dk0	121	20	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	121	21	DNA	DNA	NNP
cord-259738-yuqc6dk0	121	22	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	121	23	since	since	IN
cord-259738-yuqc6dk0	121	24	the	the	DT
cord-259738-yuqc6dk0	121	25	14th	14th	JJ
cord-259738-yuqc6dk0	121	26	-	-	HYPH
cord-259738-yuqc6dk0	121	27	day	day	NN
cord-259738-yuqc6dk0	121	28	after	after	IN
cord-259738-yuqc6dk0	121	29	first	first	JJ
cord-259738-yuqc6dk0	121	30	inoculation	inoculation	NN
cord-259738-yuqc6dk0	121	31	.	.	.
cord-259738-yuqc6dk0	122	1	The	the	DT
cord-259738-yuqc6dk0	122	2	result	result	NN
cord-259738-yuqc6dk0	122	3	suggests	suggest	VBZ
cord-259738-yuqc6dk0	122	4	that	that	IN
cord-259738-yuqc6dk0	122	5	the	the	DT
cord-259738-yuqc6dk0	122	6	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	122	7	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	122	8	-	-	HYPH
cord-259738-yuqc6dk0	122	9	N	N	NNP
cord-259738-yuqc6dk0	122	10	/	/	SYM
cord-259738-yuqc6dk0	122	11	IL2	IL2	NNP
cord-259738-yuqc6dk0	122	12	can	can	MD
cord-259738-yuqc6dk0	122	13	enhance	enhance	VB
cord-259738-yuqc6dk0	122	14	humoral	humoral	JJ
cord-259738-yuqc6dk0	122	15	responses	response	NNS
cord-259738-yuqc6dk0	122	16	(	(	-LRB-
cord-259738-yuqc6dk0	122	17	Fig	Fig	NNP
cord-259738-yuqc6dk0	122	18	.	.	NNP
cord-259738-yuqc6dk0	122	19	4	4	CD
cord-259738-yuqc6dk0	122	20	)	)	-RRB-
cord-259738-yuqc6dk0	122	21	.	.	.
cord-259738-yuqc6dk0	123	1	Peripheral	peripheral	JJ
cord-259738-yuqc6dk0	123	2	blood	blood	NN
cord-259738-yuqc6dk0	123	3	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	123	4	were	be	VBD
cord-259738-yuqc6dk0	123	5	analyzed	analyze	VBN
cord-259738-yuqc6dk0	123	6	by	by	IN
cord-259738-yuqc6dk0	123	7	flow	flow	NN
cord-259738-yuqc6dk0	123	8	cytometry	cytometry	NN
cord-259738-yuqc6dk0	123	9	on	on	IN
cord-259738-yuqc6dk0	123	10	day	day	NN
cord-259738-yuqc6dk0	123	11	7	7	CD
cord-259738-yuqc6dk0	123	12	after	after	IN
cord-259738-yuqc6dk0	123	13	the	the	DT
cord-259738-yuqc6dk0	123	14	boosting	boost	VBG
cord-259738-yuqc6dk0	123	15	immunization	immunization	NN
cord-259738-yuqc6dk0	123	16	.	.	.
cord-259738-yuqc6dk0	124	1	The	the	DT
cord-259738-yuqc6dk0	124	2	percentage	percentage	NN
cord-259738-yuqc6dk0	124	3	of	of	IN
cord-259738-yuqc6dk0	124	4	CD3	CD3	NNP
cord-259738-yuqc6dk0	124	5	+	+	CC
cord-259738-yuqc6dk0	124	6	and	and	CC
cord-259738-yuqc6dk0	124	7	CD4	CD4	NNP
cord-259738-yuqc6dk0	124	8	+	+	SYM
cord-259738-yuqc6dk0	124	9	CD3	CD3	NNP
cord-259738-yuqc6dk0	124	10	+	+	CC
cord-259738-yuqc6dk0	124	11	T	t	NN
cord-259738-yuqc6dk0	124	12	-	-	HYPH
cord-259738-yuqc6dk0	124	13	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	124	14	significantly	significantly	RB
cord-259738-yuqc6dk0	124	15	higher	high	JJR
cord-259738-yuqc6dk0	124	16	(	(	-LRB-
cord-259738-yuqc6dk0	124	17	P	p	NN
cord-259738-yuqc6dk0	124	18	<	<	XX
cord-259738-yuqc6dk0	124	19	0.05	0.05	CD
cord-259738-yuqc6dk0	124	20	)	)	-RRB-
cord-259738-yuqc6dk0	124	21	was	be	VBD
cord-259738-yuqc6dk0	124	22	observed	observe	VBN
cord-259738-yuqc6dk0	124	23	from	from	IN
cord-259738-yuqc6dk0	124	24	chickens	chicken	NNS
cord-259738-yuqc6dk0	124	25	immunized	immunize	VBN
cord-259738-yuqc6dk0	124	26	with	with	IN
cord-259738-yuqc6dk0	124	27	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	124	28	-	-	HYPH
cord-259738-yuqc6dk0	124	29	N	N	NNP
cord-259738-yuqc6dk0	124	30	/	/	SYM
cord-259738-yuqc6dk0	124	31	IL2	IL2	NNP
cord-259738-yuqc6dk0	124	32	than	than	IN
cord-259738-yuqc6dk0	124	33	the	the	DT
cord-259738-yuqc6dk0	124	34	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	124	35	-	-	HYPH
cord-259738-yuqc6dk0	124	36	N	N	NNP
cord-259738-yuqc6dk0	124	37	group	group	NN
cord-259738-yuqc6dk0	124	38	.	.	.
cord-259738-yuqc6dk0	125	1	The	the	DT
cord-259738-yuqc6dk0	125	2	percentage	percentage	NN
cord-259738-yuqc6dk0	125	3	numbers	number	NNS
cord-259738-yuqc6dk0	125	4	of	of	IN
cord-259738-yuqc6dk0	125	5	CD8	CD8	NNP
cord-259738-yuqc6dk0	125	6	+	+	SYM
cord-259738-yuqc6dk0	125	7	CD3	CD3	NNP
cord-259738-yuqc6dk0	125	8	+	+	CC
cord-259738-yuqc6dk0	125	9	T	T	NNP
cord-259738-yuqc6dk0	125	10	-	-	HYPH
cord-259738-yuqc6dk0	125	11	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	125	12	subgroups	subgroup	NNS
cord-259738-yuqc6dk0	125	13	of	of	IN
cord-259738-yuqc6dk0	125	14	the	the	DT
cord-259738-yuqc6dk0	125	15	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	125	16	-	-	HYPH
cord-259738-yuqc6dk0	125	17	N	N	NNP
cord-259738-yuqc6dk0	125	18	/	/	SYM
cord-259738-yuqc6dk0	125	19	IL2vaccinated	IL2vaccinated	NNP
cord-259738-yuqc6dk0	125	20	group	group	NN
cord-259738-yuqc6dk0	125	21	were	be	VBD
cord-259738-yuqc6dk0	125	22	higher	high	JJR
cord-259738-yuqc6dk0	125	23	than	than	IN
cord-259738-yuqc6dk0	125	24	the	the	DT
cord-259738-yuqc6dk0	125	25	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	125	26	-	-	HYPH
cord-259738-yuqc6dk0	125	27	N	N	NNP
cord-259738-yuqc6dk0	125	28	-	-	HYPH
cord-259738-yuqc6dk0	125	29	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	125	30	group	group	NN
cord-259738-yuqc6dk0	125	31	but	but	CC
cord-259738-yuqc6dk0	125	32	no	no	DT
cord-259738-yuqc6dk0	125	33	significant	significant	JJ
cord-259738-yuqc6dk0	125	34	difference	difference	NN
cord-259738-yuqc6dk0	125	35	(	(	-LRB-
cord-259738-yuqc6dk0	125	36	P	p	NN
cord-259738-yuqc6dk0	125	37	>	>	XX
cord-259738-yuqc6dk0	125	38	0.05	0.05	CD
cord-259738-yuqc6dk0	125	39	)	)	-RRB-
cord-259738-yuqc6dk0	126	1	(	(	-LRB-
cord-259738-yuqc6dk0	126	2	Fig	Fig	NNP
cord-259738-yuqc6dk0	126	3	.	.	NNP
cord-259738-yuqc6dk0	126	4	5	5	CD
cord-259738-yuqc6dk0	126	5	)	)	-RRB-
cord-259738-yuqc6dk0	126	6	.	.	.
cord-259738-yuqc6dk0	127	1	Mortality	mortality	NN
cord-259738-yuqc6dk0	127	2	,	,	,
cord-259738-yuqc6dk0	127	3	kidney	kidney	NN
cord-259738-yuqc6dk0	127	4	infection	infection	NN
cord-259738-yuqc6dk0	127	5	and	and	CC
cord-259738-yuqc6dk0	127	6	percent	percent	NN
cord-259738-yuqc6dk0	127	7	protection	protection	NN
cord-259738-yuqc6dk0	127	8	after	after	IN
cord-259738-yuqc6dk0	127	9	challenge	challenge	NN
cord-259738-yuqc6dk0	127	10	of	of	IN
cord-259738-yuqc6dk0	127	11	chickens	chicken	NNS
cord-259738-yuqc6dk0	127	12	were	be	VBD
cord-259738-yuqc6dk0	127	13	summarized	summarize	VBN
cord-259738-yuqc6dk0	127	14	in	in	IN
cord-259738-yuqc6dk0	127	15	Table	Table	NNP
cord-259738-yuqc6dk0	127	16	1	1	CD
cord-259738-yuqc6dk0	127	17	.	.	.
cord-259738-yuqc6dk0	128	1	Chickens	chicken	NNS
cord-259738-yuqc6dk0	128	2	that	that	WDT
cord-259738-yuqc6dk0	128	3	started	start	VBD
cord-259738-yuqc6dk0	128	4	to	to	TO
cord-259738-yuqc6dk0	128	5	show	show	VB
cord-259738-yuqc6dk0	128	6	clinical	clinical	JJ
cord-259738-yuqc6dk0	128	7	signs	sign	NNS
cord-259738-yuqc6dk0	128	8	or	or	CC
cord-259738-yuqc6dk0	128	9	died	die	VBD
cord-259738-yuqc6dk0	128	10	from	from	IN
cord-259738-yuqc6dk0	128	11	viral	viral	JJ
cord-259738-yuqc6dk0	128	12	infection	infection	NN
cord-259738-yuqc6dk0	128	13	did	do	VBD
cord-259738-yuqc6dk0	128	14	so	so	RB
cord-259738-yuqc6dk0	128	15	beginning	begin	VBG
cord-259738-yuqc6dk0	128	16	on	on	IN
cord-259738-yuqc6dk0	128	17	day	day	NN
cord-259738-yuqc6dk0	128	18	5	5	CD
cord-259738-yuqc6dk0	128	19	after	after	IN
cord-259738-yuqc6dk0	128	20	challenge	challenge	NN
cord-259738-yuqc6dk0	128	21	.	.	.
cord-259738-yuqc6dk0	129	1	The	the	DT
cord-259738-yuqc6dk0	129	2	chickens	chicken	NNS
cord-259738-yuqc6dk0	129	3	immunized	immunize	VBN
cord-259738-yuqc6dk0	129	4	with	with	IN
cord-259738-yuqc6dk0	129	5	either	either	DT
cord-259738-yuqc6dk0	129	6	control	control	NN
cord-259738-yuqc6dk0	129	7	vector	vector	NN
cord-259738-yuqc6dk0	129	8	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	129	9	or	or	CC
cord-259738-yuqc6dk0	129	10	PBS	PBS	NNP
cord-259738-yuqc6dk0	129	11	were	be	VBD
cord-259738-yuqc6dk0	129	12	not	not	RB
cord-259738-yuqc6dk0	129	13	protected	protect	VBN
cord-259738-yuqc6dk0	129	14	and	and	CC
cord-259738-yuqc6dk0	129	15	developed	develop	VBN
cord-259738-yuqc6dk0	129	16	coughing	coughing	NN
cord-259738-yuqc6dk0	129	17	,	,	,
cord-259738-yuqc6dk0	129	18	nasal	nasal	NN
cord-259738-yuqc6dk0	129	19	discharge	discharge	NN
cord-259738-yuqc6dk0	129	20	,	,	,
cord-259738-yuqc6dk0	129	21	dyspnea	dyspnea	NN
cord-259738-yuqc6dk0	129	22	.	.	.
cord-259738-yuqc6dk0	130	1	The	the	DT
cord-259738-yuqc6dk0	130	2	death	death	NN
cord-259738-yuqc6dk0	130	3	rate	rate	NN
cord-259738-yuqc6dk0	130	4	of	of	IN
cord-259738-yuqc6dk0	130	5	the	the	DT
cord-259738-yuqc6dk0	130	6	pIRES	pires	NN
cord-259738-yuqc6dk0	131	1	and	and	CC
cord-259738-yuqc6dk0	131	2	PBS	PBS	NNP
cord-259738-yuqc6dk0	131	3	immunized	immunize	VBD
cord-259738-yuqc6dk0	131	4	chickens	chicken	NNS
cord-259738-yuqc6dk0	131	5	was	be	VBD
cord-259738-yuqc6dk0	131	6	55	55	CD
cord-259738-yuqc6dk0	131	7	and	and	CC
cord-259738-yuqc6dk0	131	8	65	65	CD
cord-259738-yuqc6dk0	131	9	%	%	NN
cord-259738-yuqc6dk0	131	10	at	at	IN
cord-259738-yuqc6dk0	131	11	14	14	CD
cord-259738-yuqc6dk0	131	12	days	day	NNS
cord-259738-yuqc6dk0	131	13	after	after	IN
cord-259738-yuqc6dk0	131	14	challenge	challenge	NN
cord-259738-yuqc6dk0	131	15	,	,	,
cord-259738-yuqc6dk0	131	16	respectively	respectively	RB
cord-259738-yuqc6dk0	131	17	.	.	.
cord-259738-yuqc6dk0	132	1	The	the	DT
cord-259738-yuqc6dk0	132	2	death	death	NN
cord-259738-yuqc6dk0	132	3	rate	rate	NN
cord-259738-yuqc6dk0	132	4	of	of	IN
cord-259738-yuqc6dk0	132	5	the	the	DT
cord-259738-yuqc6dk0	132	6	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	132	7	-	-	HYPH
cord-259738-yuqc6dk0	132	8	N	N	NNP
cord-259738-yuqc6dk0	132	9	/	/	SYM
cord-259738-yuqc6dk0	132	10	IL2	IL2	NNP
cord-259738-yuqc6dk0	132	11	DNA	DNA	NNP
cord-259738-yuqc6dk0	132	12	vaccine	vaccine	NN
cord-259738-yuqc6dk0	132	13	immunized	immunize	VBD
cord-259738-yuqc6dk0	132	14	chickens	chicken	NNS
cord-259738-yuqc6dk0	132	15	was	be	VBD
cord-259738-yuqc6dk0	132	16	only	only	RB
cord-259738-yuqc6dk0	132	17	5	5	CD
cord-259738-yuqc6dk0	132	18	%	%	NN
cord-259738-yuqc6dk0	132	19	,	,	,
cord-259738-yuqc6dk0	132	20	lower	low	JJR
cord-259738-yuqc6dk0	132	21	than	than	IN
cord-259738-yuqc6dk0	132	22	that	that	DT
cord-259738-yuqc6dk0	132	23	of	of	IN
cord-259738-yuqc6dk0	132	24	the	the	DT
cord-259738-yuqc6dk0	132	25	chickens	chicken	NNS
cord-259738-yuqc6dk0	132	26	injected	inject	VBN
cord-259738-yuqc6dk0	132	27	with	with	IN
cord-259738-yuqc6dk0	132	28	the	the	DT
cord-259738-yuqc6dk0	132	29	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	132	30	-	-	HYPH
cord-259738-yuqc6dk0	132	31	N	N	NNP
cord-259738-yuqc6dk0	132	32	DNA	DNA	NNP
cord-259738-yuqc6dk0	132	33	vaccine	vaccine	NN
cord-259738-yuqc6dk0	132	34	.	.	.
cord-259738-yuqc6dk0	133	1	To	to	TO
cord-259738-yuqc6dk0	133	2	evaluate	evaluate	VB
cord-259738-yuqc6dk0	133	3	the	the	DT
cord-259738-yuqc6dk0	133	4	level	level	NN
cord-259738-yuqc6dk0	133	5	of	of	IN
cord-259738-yuqc6dk0	133	6	protective	protective	JJ
cord-259738-yuqc6dk0	133	7	response	response	NN
cord-259738-yuqc6dk0	133	8	after	after	IN
cord-259738-yuqc6dk0	133	9	challenge	challenge	NN
cord-259738-yuqc6dk0	133	10	,	,	,
cord-259738-yuqc6dk0	133	11	the	the	DT
cord-259738-yuqc6dk0	133	12	collected	collect	VBN
cord-259738-yuqc6dk0	133	13	kidney	kidney	NN
cord-259738-yuqc6dk0	133	14	samples	sample	NNS
cord-259738-yuqc6dk0	133	15	were	be	VBD
cord-259738-yuqc6dk0	133	16	analyzed	analyze	VBN
cord-259738-yuqc6dk0	133	17	by	by	IN
cord-259738-yuqc6dk0	133	18	RT	RT	NNP
cord-259738-yuqc6dk0	133	19	-	-	HYPH
cord-259738-yuqc6dk0	133	20	PCR	PCR	NNP
cord-259738-yuqc6dk0	133	21	.	.	.
cord-259738-yuqc6dk0	134	1	PCR	PCR	NNP
cord-259738-yuqc6dk0	134	2	results	result	NNS
cord-259738-yuqc6dk0	134	3	indicated	indicate	VBD
cord-259738-yuqc6dk0	134	4	that	that	IN
cord-259738-yuqc6dk0	134	5	20	20	CD
cord-259738-yuqc6dk0	134	6	and	and	CC
cord-259738-yuqc6dk0	134	7	50	50	CD
cord-259738-yuqc6dk0	134	8	%	%	NN
cord-259738-yuqc6dk0	134	9	of	of	IN
cord-259738-yuqc6dk0	134	10	birds	bird	NNS
cord-259738-yuqc6dk0	134	11	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	134	12	with	with	IN
cord-259738-yuqc6dk0	134	13	the	the	DT
cord-259738-yuqc6dk0	134	14	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	134	15	-	-	HYPH
cord-259738-yuqc6dk0	134	16	N	N	NNP
cord-259738-yuqc6dk0	134	17	/	/	SYM
cord-259738-yuqc6dk0	134	18	IL2	IL2	NNP
cord-259738-yuqc6dk0	134	19	and	and	CC
cord-259738-yuqc6dk0	134	20	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	134	21	-	-	HYPH
cord-259738-yuqc6dk0	134	22	N	n	CD
cord-259738-yuqc6dk0	134	23	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	134	24	were	be	VBD
cord-259738-yuqc6dk0	134	25	positive	positive	JJ
cord-259738-yuqc6dk0	134	26	for	for	IN
cord-259738-yuqc6dk0	134	27	the	the	DT
cord-259738-yuqc6dk0	134	28	presence	presence	NN
cord-259738-yuqc6dk0	134	29	of	of	IN
cord-259738-yuqc6dk0	134	30	virus	virus	NN
cord-259738-yuqc6dk0	134	31	in	in	IN
cord-259738-yuqc6dk0	134	32	the	the	DT
cord-259738-yuqc6dk0	134	33	kidney	kidney	NN
cord-259738-yuqc6dk0	134	34	,	,	,
cord-259738-yuqc6dk0	134	35	respectively	respectively	RB
cord-259738-yuqc6dk0	134	36	.	.	.
cord-259738-yuqc6dk0	135	1	All	all	DT
cord-259738-yuqc6dk0	135	2	chickens	chicken	NNS
cord-259738-yuqc6dk0	135	3	immunized	immunize	VBN
cord-259738-yuqc6dk0	135	4	with	with	IN
cord-259738-yuqc6dk0	135	5	either	either	DT
cord-259738-yuqc6dk0	135	6	control	control	NN
cord-259738-yuqc6dk0	135	7	vector	vector	NN
cord-259738-yuqc6dk0	135	8	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	135	9	or	or	CC
cord-259738-yuqc6dk0	135	10	PBS	PBS	NNP
cord-259738-yuqc6dk0	135	11	were	be	VBD
cord-259738-yuqc6dk0	135	12	positive	positive	JJ
cord-259738-yuqc6dk0	135	13	in	in	IN
cord-259738-yuqc6dk0	135	14	RT	RT	NNP
cord-259738-yuqc6dk0	135	15	-	-	HYPH
cord-259738-yuqc6dk0	135	16	PCR	PCR	NNP
cord-259738-yuqc6dk0	135	17	test	test	NN
cord-259738-yuqc6dk0	135	18	.	.	.
cord-259738-yuqc6dk0	136	1	The	the	DT
cord-259738-yuqc6dk0	136	2	protection	protection	NN
cord-259738-yuqc6dk0	136	3	percent	percent	NN
cord-259738-yuqc6dk0	136	4	of	of	IN
cord-259738-yuqc6dk0	136	5	group	group	NN
cord-259738-yuqc6dk0	136	6	that	that	WDT
cord-259738-yuqc6dk0	136	7	vaccinated	vaccinate	VBD
cord-259738-yuqc6dk0	136	8	with	with	IN
cord-259738-yuqc6dk0	136	9	the	the	DT
cord-259738-yuqc6dk0	136	10	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	136	11	-	-	HYPH
cord-259738-yuqc6dk0	136	12	N	N	NNP
cord-259738-yuqc6dk0	136	13	alone	alone	RB
cord-259738-yuqc6dk0	136	14	was	be	VBD
cord-259738-yuqc6dk0	136	15	higher	high	JJR
cord-259738-yuqc6dk0	136	16	than	than	IN
cord-259738-yuqc6dk0	136	17	that	that	DT
cord-259738-yuqc6dk0	136	18	of	of	IN
cord-259738-yuqc6dk0	136	19	the	the	DT
cord-259738-yuqc6dk0	136	20	empty	empty	JJ
cord-259738-yuqc6dk0	136	21	vector	vector	NN
cord-259738-yuqc6dk0	136	22	or	or	CC
cord-259738-yuqc6dk0	136	23	PBS	PBS	NNP
cord-259738-yuqc6dk0	136	24	.	.	.
cord-259738-yuqc6dk0	137	1	The	the	DT
cord-259738-yuqc6dk0	137	2	group	group	NN
cord-259738-yuqc6dk0	137	3	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	137	4	with	with	IN
cord-259738-yuqc6dk0	137	5	the	the	DT
cord-259738-yuqc6dk0	137	6	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	137	7	-	-	HYPH
cord-259738-yuqc6dk0	137	8	N	N	NNP
cord-259738-yuqc6dk0	137	9	/	/	SYM
cord-259738-yuqc6dk0	137	10	IL2	IL2	NNP
cord-259738-yuqc6dk0	137	11	DNA	DNA	NNP
cord-259738-yuqc6dk0	137	12	vaccine	vaccine	NN
cord-259738-yuqc6dk0	137	13	(	(	-LRB-
cord-259738-yuqc6dk0	137	14	80	80	CD
cord-259738-yuqc6dk0	137	15	%	%	NN
cord-259738-yuqc6dk0	137	16	)	)	-RRB-
cord-259738-yuqc6dk0	137	17	had	have	VBD
cord-259738-yuqc6dk0	137	18	the	the	DT
cord-259738-yuqc6dk0	137	19	highest	high	JJS
cord-259738-yuqc6dk0	137	20	protection	protection	NN
cord-259738-yuqc6dk0	137	21	rate	rate	NN
cord-259738-yuqc6dk0	137	22	in	in	IN
cord-259738-yuqc6dk0	137	23	all	all	DT
cord-259738-yuqc6dk0	137	24	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	137	25	groups	group	NNS
cord-259738-yuqc6dk0	137	26	.	.	.
cord-259738-yuqc6dk0	138	1	This	this	DT
cord-259738-yuqc6dk0	138	2	suggests	suggest	VBZ
cord-259738-yuqc6dk0	138	3	that	that	IN
cord-259738-yuqc6dk0	138	4	the	the	DT
cord-259738-yuqc6dk0	138	5	plasmid	plasmid	NN
cord-259738-yuqc6dk0	138	6	expressing	express	VBG
cord-259738-yuqc6dk0	138	7	both	both	CC
cord-259738-yuqc6dk0	138	8	N	n	CD
cord-259738-yuqc6dk0	138	9	protein	protein	NN
cord-259738-yuqc6dk0	138	10	and	and	CC
cord-259738-yuqc6dk0	138	11	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	138	12	offers	offer	VBZ
cord-259738-yuqc6dk0	138	13	enhanced	enhanced	JJ
cord-259738-yuqc6dk0	138	14	resistance	resistance	NN
cord-259738-yuqc6dk0	138	15	against	against	IN
cord-259738-yuqc6dk0	138	16	a	a	DT
cord-259738-yuqc6dk0	138	17	virulent	virulent	JJ
cord-259738-yuqc6dk0	138	18	IBV	IBV	NNP
cord-259738-yuqc6dk0	138	19	challenge	challenge	NN
cord-259738-yuqc6dk0	138	20	.	.	.
cord-259738-yuqc6dk0	139	1	In	in	IN
cord-259738-yuqc6dk0	139	2	order	order	NN
cord-259738-yuqc6dk0	139	3	to	to	TO
cord-259738-yuqc6dk0	139	4	increase	increase	VB
cord-259738-yuqc6dk0	139	5	the	the	DT
cord-259738-yuqc6dk0	139	6	efficiency	efficiency	NN
cord-259738-yuqc6dk0	139	7	of	of	IN
cord-259738-yuqc6dk0	139	8	this	this	DT
cord-259738-yuqc6dk0	139	9	immunization	immunization	NN
cord-259738-yuqc6dk0	139	10	procedure	procedure	NN
cord-259738-yuqc6dk0	139	11	,	,	,
cord-259738-yuqc6dk0	139	12	cytokine	cytokine	NN
cord-259738-yuqc6dk0	139	13	was	be	VBD
cord-259738-yuqc6dk0	139	14	co	co	VBN
cord-259738-yuqc6dk0	139	15	-	-	VBN
cord-259738-yuqc6dk0	139	16	expressed	express	VBN
cord-259738-yuqc6dk0	139	17	with	with	IN
cord-259738-yuqc6dk0	139	18	the	the	DT
cord-259738-yuqc6dk0	139	19	viral	viral	JJ
cord-259738-yuqc6dk0	139	20	protein	protein	NN
cord-259738-yuqc6dk0	139	21	.	.	.
cord-259738-yuqc6dk0	140	1	This	this	DT
cord-259738-yuqc6dk0	140	2	strategy	strategy	NN
cord-259738-yuqc6dk0	140	3	has	have	VBZ
cord-259738-yuqc6dk0	140	4	already	already	RB
cord-259738-yuqc6dk0	140	5	been	be	VBN
cord-259738-yuqc6dk0	140	6	used	use	VBN
cord-259738-yuqc6dk0	140	7	successfully	successfully	RB
cord-259738-yuqc6dk0	140	8	to	to	TO
cord-259738-yuqc6dk0	140	9	enhance	enhance	VB
cord-259738-yuqc6dk0	140	10	the	the	DT
cord-259738-yuqc6dk0	140	11	effect	effect	NN
cord-259738-yuqc6dk0	140	12	of	of	IN
cord-259738-yuqc6dk0	140	13	DNA	dna	NN
cord-259738-yuqc6dk0	140	14	vaccination	vaccination	NN
cord-259738-yuqc6dk0	140	15	procedures	procedure	NNS
cord-259738-yuqc6dk0	141	1	(	(	-LRB-
cord-259738-yuqc6dk0	141	2	Li	Li	NNP
cord-259738-yuqc6dk0	141	3	et	et	NNP
cord-259738-yuqc6dk0	141	4	al	al	NNP
cord-259738-yuqc6dk0	141	5	.	.	NNP
cord-259738-yuqc6dk0	141	6	,	,	,
cord-259738-yuqc6dk0	141	7	2006	2006	CD
cord-259738-yuqc6dk0	142	1	;	;	:
cord-259738-yuqc6dk0	142	2	Chow	Chow	NNP
cord-259738-yuqc6dk0	142	3	et	et	NNP
cord-259738-yuqc6dk0	142	4	al	al	NNP
cord-259738-yuqc6dk0	142	5	.	.	NNP
cord-259738-yuqc6dk0	142	6	,	,	,
cord-259738-yuqc6dk0	142	7	1997	1997	CD
cord-259738-yuqc6dk0	142	8	;	;	:
cord-259738-yuqc6dk0	142	9	Henke	Henke	NNP
cord-259738-yuqc6dk0	142	10	et	et	NNP
cord-259738-yuqc6dk0	142	11	al	al	NNP
cord-259738-yuqc6dk0	142	12	.	.	NNP
cord-259738-yuqc6dk0	142	13	,	,	,
cord-259738-yuqc6dk0	142	14	2004	2004	CD
cord-259738-yuqc6dk0	142	15	)	)	-RRB-
cord-259738-yuqc6dk0	142	16	.	.	.
cord-259738-yuqc6dk0	143	1	The	the	DT
cord-259738-yuqc6dk0	143	2	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	143	3	-	-	HYPH
cord-259738-yuqc6dk0	143	4	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	143	5	/	/	SYM
cord-259738-yuqc6dk0	143	6	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	143	7	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	143	8	plasmid	plasmid	NN
cord-259738-yuqc6dk0	143	9	which	which	WDT
cord-259738-yuqc6dk0	143	10	was	be	VBD
cord-259738-yuqc6dk0	143	11	constructed	construct	VBN
cord-259738-yuqc6dk0	143	12	and	and	CC
cord-259738-yuqc6dk0	143	13	identified	identify	VBN
cord-259738-yuqc6dk0	143	14	previously	previously	RB
cord-259738-yuqc6dk0	143	15	was	be	VBD
cord-259738-yuqc6dk0	143	16	used	use	VBN
cord-259738-yuqc6dk0	143	17	to	to	TO
cord-259738-yuqc6dk0	143	18	construct	construct	VB
cord-259738-yuqc6dk0	143	19	monocistronic	monocistronic	NNP
cord-259738-yuqc6dk0	143	20	and	and	CC
cord-259738-yuqc6dk0	143	21	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	143	22	DNA	DNA	NNP
cord-259738-yuqc6dk0	143	23	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	143	24	.	.	.
cord-259738-yuqc6dk0	144	1	The	the	DT
cord-259738-yuqc6dk0	144	2	plasmid	plasmid	NN
cord-259738-yuqc6dk0	144	3	pIRES	pires	NN
cord-259738-yuqc6dk0	144	4	-	-	HYPH
cord-259738-yuqc6dk0	144	5	EGFP	EGFP	NNP
cord-259738-yuqc6dk0	144	6	/	/	SYM
cord-259738-yuqc6dk0	144	7	DsRed	DsRed	NNP
cord-259738-yuqc6dk0	144	8	enabled	enable	VBD
cord-259738-yuqc6dk0	144	9	the	the	DT
cord-259738-yuqc6dk0	144	10	simultaneous	simultaneous	JJ
cord-259738-yuqc6dk0	144	11	translation	translation	NN
cord-259738-yuqc6dk0	144	12	of	of	IN
cord-259738-yuqc6dk0	144	13	two	two	CD
cord-259738-yuqc6dk0	144	14	genes	gene	NNS
cord-259738-yuqc6dk0	144	15	of	of	IN
cord-259738-yuqc6dk0	144	16	interest	interest	NN
cord-259738-yuqc6dk0	144	17	from	from	IN
cord-259738-yuqc6dk0	144	18	the	the	DT
cord-259738-yuqc6dk0	144	19	same	same	JJ
cord-259738-yuqc6dk0	144	20	RNA	RNA	NNP
cord-259738-yuqc6dk0	144	21	transcript	transcript	NN
cord-259738-yuqc6dk0	144	22	.	.	.
cord-259738-yuqc6dk0	145	1	Each	each	DT
cord-259738-yuqc6dk0	145	2	gene	gene	NN
cord-259738-yuqc6dk0	145	3	is	be	VBZ
cord-259738-yuqc6dk0	145	4	cloned	clone	VBN
cord-259738-yuqc6dk0	145	5	into	into	IN
cord-259738-yuqc6dk0	145	6	one	one	CD
cord-259738-yuqc6dk0	145	7	of	of	IN
cord-259738-yuqc6dk0	145	8	the	the	DT
cord-259738-yuqc6dk0	145	9	multiple	multiple	JJ
cord-259738-yuqc6dk0	145	10	cloning	clone	VBG
cord-259738-yuqc6dk0	145	11	sites	site	NNS
cord-259738-yuqc6dk0	145	12	on	on	IN
cord-259738-yuqc6dk0	145	13	either	either	DT
cord-259738-yuqc6dk0	145	14	side	side	NN
cord-259738-yuqc6dk0	145	15	of	of	IN
cord-259738-yuqc6dk0	145	16	the	the	DT
cord-259738-yuqc6dk0	145	17	internal	internal	JJ
cord-259738-yuqc6dk0	145	18	ribosomal	ribosomal	JJ
cord-259738-yuqc6dk0	145	19	entry	entry	NN
cord-259738-yuqc6dk0	145	20	site	site	NN
cord-259738-yuqc6dk0	145	21	of	of	IN
cord-259738-yuqc6dk0	145	22	the	the	DT
cord-259738-yuqc6dk0	145	23	encephalomyocarditis	encephalomyocarditis	NNP
cord-259738-yuqc6dk0	145	24	virus	virus	NN
cord-259738-yuqc6dk0	145	25	(	(	-LRB-
cord-259738-yuqc6dk0	145	26	EMCV	EMCV	NNP
cord-259738-yuqc6dk0	145	27	)	)	-RRB-
cord-259738-yuqc6dk0	145	28	.	.	.
cord-259738-yuqc6dk0	146	1	The	the	DT
cord-259738-yuqc6dk0	146	2	entire	entire	JJ
cord-259738-yuqc6dk0	146	3	construct	construct	NN
cord-259738-yuqc6dk0	146	4	is	be	VBZ
cord-259738-yuqc6dk0	146	5	under	under	IN
cord-259738-yuqc6dk0	146	6	control	control	NN
cord-259738-yuqc6dk0	146	7	of	of	IN
cord-259738-yuqc6dk0	146	8	the	the	DT
cord-259738-yuqc6dk0	146	9	cytomegalovirus	cytomegalovirus	NNP
cord-259738-yuqc6dk0	146	10	(	(	-LRB-
cord-259738-yuqc6dk0	146	11	CMV	CMV	NNP
cord-259738-yuqc6dk0	146	12	)	)	-RRB-
cord-259738-yuqc6dk0	146	13	immediate	immediate	JJ
cord-259738-yuqc6dk0	146	14	-	-	HYPH
cord-259738-yuqc6dk0	146	15	early	early	JJ
cord-259738-yuqc6dk0	146	16	promoter	promoter	NN
cord-259738-yuqc6dk0	146	17	allowing	allow	VBG
cord-259738-yuqc6dk0	146	18	the	the	DT
cord-259738-yuqc6dk0	146	19	expression	expression	NN
cord-259738-yuqc6dk0	146	20	of	of	IN
cord-259738-yuqc6dk0	146	21	two	two	CD
cord-259738-yuqc6dk0	146	22	individual	individual	JJ
cord-259738-yuqc6dk0	146	23	proteins	protein	NNS
cord-259738-yuqc6dk0	146	24	from	from	IN
cord-259738-yuqc6dk0	146	25	one	one	CD
cord-259738-yuqc6dk0	146	26	plasmid	plasmid	NN
cord-259738-yuqc6dk0	146	27	.	.	.
cord-259738-yuqc6dk0	147	1	In	in	IN
cord-259738-yuqc6dk0	147	2	this	this	DT
cord-259738-yuqc6dk0	147	3	study	study	NN
cord-259738-yuqc6dk0	147	4	,	,	,
cord-259738-yuqc6dk0	147	5	an	an	DT
cord-259738-yuqc6dk0	147	6	experimental	experimental	JJ
cord-259738-yuqc6dk0	147	7	immunization	immunization	NN
cord-259738-yuqc6dk0	147	8	strategy	strategy	NN
cord-259738-yuqc6dk0	147	9	was	be	VBD
cord-259738-yuqc6dk0	147	10	developed	develop	VBN
cord-259738-yuqc6dk0	147	11	and	and	CC
cord-259738-yuqc6dk0	147	12	tested	test	VBN
cord-259738-yuqc6dk0	147	13	against	against	IN
cord-259738-yuqc6dk0	147	14	IBV	IBV	NNP
cord-259738-yuqc6dk0	147	15	.	.	.
cord-259738-yuqc6dk0	148	1	Two	two	CD
cord-259738-yuqc6dk0	148	2	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	148	3	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	148	4	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	148	5	-	-	HYPH
cord-259738-yuqc6dk0	148	6	N	N	NNP
cord-259738-yuqc6dk0	148	7	/	/	SYM
cord-259738-yuqc6dk0	148	8	IL2	IL2	NNP
cord-259738-yuqc6dk0	148	9	,	,	,
cord-259738-yuqc6dk0	148	10	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	148	11	-	-	HYPH
cord-259738-yuqc6dk0	148	12	N	N	NNP
cord-259738-yuqc6dk0	148	13	were	be	VBD
cord-259738-yuqc6dk0	148	14	constructed	construct	VBN
cord-259738-yuqc6dk0	148	15	.	.	.
cord-259738-yuqc6dk0	149	1	These	these	DT
cord-259738-yuqc6dk0	149	2	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	149	3	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	149	4	were	be	VBD
cord-259738-yuqc6dk0	149	5	inoculated	inoculate	VBN
cord-259738-yuqc6dk0	149	6	in	in	IN
cord-259738-yuqc6dk0	149	7	chickens	chicken	NNS
cord-259738-yuqc6dk0	149	8	and	and	CC
cord-259738-yuqc6dk0	149	9	tested	test	VBN
cord-259738-yuqc6dk0	149	10	in	in	IN
cord-259738-yuqc6dk0	149	11	a	a	DT
cord-259738-yuqc6dk0	149	12	protection	protection	NN
cord-259738-yuqc6dk0	149	13	-	-	HYPH
cord-259738-yuqc6dk0	149	14	challenge	challenge	NN
cord-259738-yuqc6dk0	149	15	experiment	experiment	NN
cord-259738-yuqc6dk0	149	16	,	,	,
cord-259738-yuqc6dk0	149	17	demonstrating	demonstrate	VBG
cord-259738-yuqc6dk0	149	18	that	that	DT
cord-259738-yuqc6dk0	149	19	vaccination	vaccination	NN
cord-259738-yuqc6dk0	149	20	with	with	IN
cord-259738-yuqc6dk0	149	21	the	the	DT
cord-259738-yuqc6dk0	149	22	co	co	JJ
cord-259738-yuqc6dk0	149	23	-	-	JJ
cord-259738-yuqc6dk0	149	24	expression	expression	JJ
cord-259738-yuqc6dk0	149	25	plasmid	plasmid	NN
cord-259738-yuqc6dk0	150	1	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	150	2	-	-	HYPH
cord-259738-yuqc6dk0	150	3	N	N	NNP
cord-259738-yuqc6dk0	150	4	/	/	SYM
cord-259738-yuqc6dk0	150	5	IL2	IL2	NNP
cord-259738-yuqc6dk0	150	6	can	can	MD
cord-259738-yuqc6dk0	150	7	induce	induce	VB
cord-259738-yuqc6dk0	150	8	stronger	strong	JJR
cord-259738-yuqc6dk0	150	9	immune	immune	JJ
cord-259738-yuqc6dk0	150	10	response	response	NN
cord-259738-yuqc6dk0	150	11	than	than	IN
cord-259738-yuqc6dk0	150	12	vaccination	vaccination	NN
cord-259738-yuqc6dk0	150	13	with	with	IN
cord-259738-yuqc6dk0	151	1	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	151	2	-	-	HYPH
cord-259738-yuqc6dk0	151	3	N.	N.	NNP
cord-259738-yuqc6dk0	151	4	Thus	thus	RB
cord-259738-yuqc6dk0	151	5	,	,	,
cord-259738-yuqc6dk0	151	6	it	-PRON-	PRP
cord-259738-yuqc6dk0	151	7	seems	seem	VBZ
cord-259738-yuqc6dk0	151	8	that	that	IN
cord-259738-yuqc6dk0	151	9	vaccination	vaccination	NN
cord-259738-yuqc6dk0	151	10	with	with	IN
cord-259738-yuqc6dk0	151	11	a	a	DT
cord-259738-yuqc6dk0	151	12	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	151	13	DNA	dna	NN
cord-259738-yuqc6dk0	151	14	vaccine	vaccine	NN
cord-259738-yuqc6dk0	151	15	expressing	express	VBG
cord-259738-yuqc6dk0	151	16	both	both	CC
cord-259738-yuqc6dk0	151	17	IBV	IBV	NNP
cord-259738-yuqc6dk0	151	18	N	N	NNP
cord-259738-yuqc6dk0	151	19	protein	protein	NN
cord-259738-yuqc6dk0	151	20	and	and	CC
cord-259738-yuqc6dk0	151	21	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	151	22	may	may	MD
cord-259738-yuqc6dk0	151	23	elicit	elicit	VB
cord-259738-yuqc6dk0	151	24	potent	potent	JJ
cord-259738-yuqc6dk0	151	25	immune	immune	JJ
cord-259738-yuqc6dk0	151	26	response	response	NN
cord-259738-yuqc6dk0	151	27	.	.	.
cord-259738-yuqc6dk0	152	1	In	in	IN
cord-259738-yuqc6dk0	152	2	this	this	DT
cord-259738-yuqc6dk0	152	3	study	study	NN
cord-259738-yuqc6dk0	152	4	,	,	,
cord-259738-yuqc6dk0	152	5	the	the	DT
cord-259738-yuqc6dk0	152	6	level	level	NN
cord-259738-yuqc6dk0	152	7	of	of	IN
cord-259738-yuqc6dk0	152	8	specific	specific	JJ
cord-259738-yuqc6dk0	152	9	antibodies	antibody	NNS
cord-259738-yuqc6dk0	152	10	developed	develop	VBN
cord-259738-yuqc6dk0	152	11	in	in	IN
cord-259738-yuqc6dk0	152	12	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	152	13	-	-	HYPH
cord-259738-yuqc6dk0	152	14	N	N	NNP
cord-259738-yuqc6dk0	152	15	/	/	SYM
cord-259738-yuqc6dk0	152	16	IL2	IL2	NNP
cord-259738-yuqc6dk0	152	17	group	group	NN
cord-259738-yuqc6dk0	152	18	was	be	VBD
cord-259738-yuqc6dk0	152	19	higher	high	JJR
cord-259738-yuqc6dk0	152	20	than	than	IN
cord-259738-yuqc6dk0	152	21	that	that	DT
cord-259738-yuqc6dk0	152	22	of	of	IN
cord-259738-yuqc6dk0	152	23	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	152	24	-	-	HYPH
cord-259738-yuqc6dk0	152	25	N	N	NNP
cord-259738-yuqc6dk0	152	26	group	group	NN
cord-259738-yuqc6dk0	152	27	.	.	.
cord-259738-yuqc6dk0	153	1	However	however	RB
cord-259738-yuqc6dk0	153	2	,	,	,
cord-259738-yuqc6dk0	153	3	the	the	DT
cord-259738-yuqc6dk0	153	4	precise	precise	JJ
cord-259738-yuqc6dk0	153	5	role	role	NN
cord-259738-yuqc6dk0	153	6	of	of	IN
cord-259738-yuqc6dk0	153	7	antibodies	antibody	NNS
cord-259738-yuqc6dk0	153	8	for	for	IN
cord-259738-yuqc6dk0	153	9	the	the	DT
cord-259738-yuqc6dk0	153	10	control	control	NN
cord-259738-yuqc6dk0	153	11	of	of	IN
cord-259738-yuqc6dk0	153	12	IBV	IBV	NNP
cord-259738-yuqc6dk0	153	13	infection	infection	NN
cord-259738-yuqc6dk0	153	14	remains	remain	VBZ
cord-259738-yuqc6dk0	153	15	controversial	controversial	JJ
cord-259738-yuqc6dk0	153	16	.	.	.
cord-259738-yuqc6dk0	154	1	Some	some	DT
cord-259738-yuqc6dk0	154	2	reports	report	NNS
cord-259738-yuqc6dk0	154	3	have	have	VBP
cord-259738-yuqc6dk0	154	4	shown	show	VBN
cord-259738-yuqc6dk0	154	5	that	that	IN
cord-259738-yuqc6dk0	154	6	circulating	circulate	VBG
cord-259738-yuqc6dk0	154	7	antibody	antibody	NN
cord-259738-yuqc6dk0	154	8	titer	titer	NN
cord-259738-yuqc6dk0	154	9	did	do	VBD
cord-259738-yuqc6dk0	154	10	not	not	RB
cord-259738-yuqc6dk0	154	11	correlate	correlate	VB
cord-259738-yuqc6dk0	154	12	with	with	IN
cord-259738-yuqc6dk0	154	13	protection	protection	NN
cord-259738-yuqc6dk0	154	14	from	from	IN
cord-259738-yuqc6dk0	154	15	IBV	IBV	NNP
cord-259738-yuqc6dk0	154	16	infection	infection	NN
cord-259738-yuqc6dk0	155	1	(	(	-LRB-
cord-259738-yuqc6dk0	155	2	Gelb	Gelb	NNP
cord-259738-yuqc6dk0	155	3	et	et	NNP
cord-259738-yuqc6dk0	155	4	al	al	NNP
cord-259738-yuqc6dk0	155	5	.	.	NNP
cord-259738-yuqc6dk0	155	6	,	,	,
cord-259738-yuqc6dk0	155	7	1998	1998	CD
cord-259738-yuqc6dk0	155	8	;	;	:
cord-259738-yuqc6dk0	155	9	Gough	Gough	NNP
cord-259738-yuqc6dk0	155	10	and	and	CC
cord-259738-yuqc6dk0	155	11	Alexander	Alexander	NNP
cord-259738-yuqc6dk0	155	12	,	,	,
cord-259738-yuqc6dk0	155	13	1979	1979	CD
cord-259738-yuqc6dk0	155	14	;	;	:
cord-259738-yuqc6dk0	155	15	Raggi	Raggi	NNP
cord-259738-yuqc6dk0	155	16	and	and	CC
cord-259738-yuqc6dk0	155	17	Lee	Lee	NNP
cord-259738-yuqc6dk0	155	18	,	,	,
cord-259738-yuqc6dk0	155	19	1965	1965	CD
cord-259738-yuqc6dk0	155	20	)	)	-RRB-
cord-259738-yuqc6dk0	155	21	.	.	.
cord-259738-yuqc6dk0	156	1	Other	other	JJ
cord-259738-yuqc6dk0	156	2	studies	study	NNS
cord-259738-yuqc6dk0	156	3	demonstrated	demonstrate	VBD
cord-259738-yuqc6dk0	156	4	that	that	IN
cord-259738-yuqc6dk0	156	5	humoral	humoral	JJ
cord-259738-yuqc6dk0	156	6	immunity	immunity	NN
cord-259738-yuqc6dk0	156	7	plays	play	VBZ
cord-259738-yuqc6dk0	156	8	an	an	DT
cord-259738-yuqc6dk0	156	9	important	important	JJ
cord-259738-yuqc6dk0	156	10	role	role	NN
cord-259738-yuqc6dk0	156	11	in	in	IN
cord-259738-yuqc6dk0	156	12	disease	disease	NN
cord-259738-yuqc6dk0	156	13	recovery	recovery	NN
cord-259738-yuqc6dk0	156	14	and	and	CC
cord-259738-yuqc6dk0	156	15	virus	virus	NN
cord-259738-yuqc6dk0	156	16	clearance	clearance	NN
cord-259738-yuqc6dk0	157	1	(	(	-LRB-
cord-259738-yuqc6dk0	157	2	Cook	Cook	NNP
cord-259738-yuqc6dk0	157	3	et	et	NNP
cord-259738-yuqc6dk0	157	4	al	al	NNP
cord-259738-yuqc6dk0	157	5	.	.	NNP
cord-259738-yuqc6dk0	157	6	,	,	,
cord-259738-yuqc6dk0	157	7	1991	1991	CD
cord-259738-yuqc6dk0	157	8	;	;	:
cord-259738-yuqc6dk0	157	9	Thompson	Thompson	NNP
cord-259738-yuqc6dk0	157	10	et	et	NNP
cord-259738-yuqc6dk0	157	11	al	al	NNP
cord-259738-yuqc6dk0	157	12	.	.	NNP
cord-259738-yuqc6dk0	157	13	,	,	,
cord-259738-yuqc6dk0	157	14	1997	1997	CD
cord-259738-yuqc6dk0	157	15	;	;	.
cord-259738-yuqc6dk0	158	1	Toro	Toro	NNP
cord-259738-yuqc6dk0	158	2	and	and	CC
cord-259738-yuqc6dk0	158	3	Fernandez	Fernandez	NNP
cord-259738-yuqc6dk0	158	4	,	,	,
cord-259738-yuqc6dk0	158	5	1994	1994	CD
cord-259738-yuqc6dk0	158	6	)	)	-RRB-
cord-259738-yuqc6dk0	158	7	.	.	.
cord-259738-yuqc6dk0	159	1	The	the	DT
cord-259738-yuqc6dk0	159	2	antibody	antibody	NN
cord-259738-yuqc6dk0	159	3	-	-	HYPH
cord-259738-yuqc6dk0	159	4	dependence	dependence	NN
cord-259738-yuqc6dk0	159	5	of	of	IN
cord-259738-yuqc6dk0	159	6	the	the	DT
cord-259738-yuqc6dk0	159	7	mechanism	mechanism	NN
cord-259738-yuqc6dk0	159	8	of	of	IN
cord-259738-yuqc6dk0	159	9	protection	protection	NN
cord-259738-yuqc6dk0	159	10	against	against	IN
cord-259738-yuqc6dk0	159	11	the	the	DT
cord-259738-yuqc6dk0	159	12	disease	disease	NN
cord-259738-yuqc6dk0	159	13	remains	remain	VBZ
cord-259738-yuqc6dk0	159	14	unclear	unclear	JJ
cord-259738-yuqc6dk0	159	15	,	,	,
cord-259738-yuqc6dk0	159	16	suggesting	suggest	VBG
cord-259738-yuqc6dk0	159	17	an	an	DT
cord-259738-yuqc6dk0	159	18	important	important	JJ
cord-259738-yuqc6dk0	159	19	role	role	NN
cord-259738-yuqc6dk0	159	20	of	of	IN
cord-259738-yuqc6dk0	159	21	the	the	DT
cord-259738-yuqc6dk0	159	22	T	t	NN
cord-259738-yuqc6dk0	159	23	-	-	HYPH
cord-259738-yuqc6dk0	159	24	cell	cell	NN
cord-259738-yuqc6dk0	159	25	response	response	NN
cord-259738-yuqc6dk0	159	26	in	in	IN
cord-259738-yuqc6dk0	159	27	efficacy	efficacy	NN
cord-259738-yuqc6dk0	159	28	protection	protection	NN
cord-259738-yuqc6dk0	159	29	.	.	.
cord-259738-yuqc6dk0	160	1	In	in	IN
cord-259738-yuqc6dk0	160	2	order	order	NN
cord-259738-yuqc6dk0	160	3	to	to	TO
cord-259738-yuqc6dk0	160	4	evaluate	evaluate	VB
cord-259738-yuqc6dk0	160	5	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	160	6	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	160	7	induced	induce	VBN
cord-259738-yuqc6dk0	160	8	T	t	NN
cord-259738-yuqc6dk0	160	9	-	-	HYPH
cord-259738-yuqc6dk0	160	10	cell	cell	NN
cord-259738-yuqc6dk0	160	11	response	response	NN
cord-259738-yuqc6dk0	160	12	,	,	,
cord-259738-yuqc6dk0	160	13	peripheral	peripheral	JJ
cord-259738-yuqc6dk0	160	14	blood	blood	NN
cord-259738-yuqc6dk0	160	15	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	160	16	were	be	VBD
cord-259738-yuqc6dk0	160	17	analyzed	analyze	VBN
cord-259738-yuqc6dk0	160	18	by	by	IN
cord-259738-yuqc6dk0	160	19	flow	flow	NN
cord-259738-yuqc6dk0	160	20	cytometry	cytometry	NN
cord-259738-yuqc6dk0	160	21	.	.	.
cord-259738-yuqc6dk0	161	1	Results	result	NNS
cord-259738-yuqc6dk0	161	2	of	of	IN
cord-259738-yuqc6dk0	161	3	T	T	NNP
cord-259738-yuqc6dk0	161	4	-	-	HYPH
cord-259738-yuqc6dk0	161	5	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	161	6	subgroup	subgroup	NNP
cord-259738-yuqc6dk0	161	7	detection	detection	NN
cord-259738-yuqc6dk0	161	8	indicated	indicate	VBD
cord-259738-yuqc6dk0	161	9	,	,	,
cord-259738-yuqc6dk0	161	10	that	that	IN
cord-259738-yuqc6dk0	161	11	the	the	DT
cord-259738-yuqc6dk0	161	12	percentage	percentage	NN
cord-259738-yuqc6dk0	161	13	numbers	number	NNS
cord-259738-yuqc6dk0	161	14	of	of	IN
cord-259738-yuqc6dk0	161	15	CD3	CD3	NNP
cord-259738-yuqc6dk0	161	16	+	+	CC
cord-259738-yuqc6dk0	161	17	,	,	,
cord-259738-yuqc6dk0	161	18	CD4	CD4	NNP
cord-259738-yuqc6dk0	161	19	+	+	CC
cord-259738-yuqc6dk0	161	20	CD3	CD3	NNP
cord-259738-yuqc6dk0	161	21	+	+	CC
cord-259738-yuqc6dk0	161	22	and	and	CC
cord-259738-yuqc6dk0	161	23	CD8	CD8	NNP
cord-259738-yuqc6dk0	161	24	+	+	SYM
cord-259738-yuqc6dk0	161	25	CD3	CD3	NNP
cord-259738-yuqc6dk0	161	26	+	+	CC
cord-259738-yuqc6dk0	161	27	T	T	NNP
cord-259738-yuqc6dk0	161	28	-	-	HYPH
cord-259738-yuqc6dk0	161	29	lymphocytes	lymphocyte	VBZ
cord-259738-yuqc6dk0	161	30	subgroups	subgroup	NNS
cord-259738-yuqc6dk0	161	31	in	in	IN
cord-259738-yuqc6dk0	162	1	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	162	2	-	-	HYPH
cord-259738-yuqc6dk0	162	3	N	N	NNP
cord-259738-yuqc6dk0	162	4	/	/	SYM
cord-259738-yuqc6dk0	162	5	IL2	IL2	NNP
cord-259738-yuqc6dk0	162	6	vaccinated	vaccinate	VBD
cord-259738-yuqc6dk0	162	7	chickens	chicken	NNS
cord-259738-yuqc6dk0	162	8	group	group	NN
cord-259738-yuqc6dk0	162	9	were	be	VBD
cord-259738-yuqc6dk0	162	10	higher	high	JJR
cord-259738-yuqc6dk0	162	11	than	than	IN
cord-259738-yuqc6dk0	162	12	those	those	DT
cord-259738-yuqc6dk0	162	13	in	in	IN
cord-259738-yuqc6dk0	162	14	the	the	DT
cord-259738-yuqc6dk0	162	15	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	162	16	-	-	HYPH
cord-259738-yuqc6dk0	162	17	N	N	NNP
cord-259738-yuqc6dk0	162	18	vaccinated	vaccinate	VBD
cord-259738-yuqc6dk0	162	19	chickens	chicken	NNS
cord-259738-yuqc6dk0	162	20	.	.	.
cord-259738-yuqc6dk0	163	1	This	this	DT
cord-259738-yuqc6dk0	163	2	demonstrated	demonstrate	VBD
cord-259738-yuqc6dk0	163	3	that	that	IN
cord-259738-yuqc6dk0	163	4	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	163	5	has	have	VBZ
cord-259738-yuqc6dk0	163	6	the	the	DT
cord-259738-yuqc6dk0	163	7	ability	ability	NN
cord-259738-yuqc6dk0	163	8	to	to	TO
cord-259738-yuqc6dk0	163	9	stimulate	stimulate	VB
cord-259738-yuqc6dk0	163	10	T	t	NN
cord-259738-yuqc6dk0	163	11	-	-	HYPH
cord-259738-yuqc6dk0	163	12	cell	cell	NN
cord-259738-yuqc6dk0	163	13	growth	growth	NN
cord-259738-yuqc6dk0	163	14	.	.	.
cord-259738-yuqc6dk0	164	1	It	-PRON-	PRP
cord-259738-yuqc6dk0	164	2	has	have	VBZ
cord-259738-yuqc6dk0	164	3	been	be	VBN
cord-259738-yuqc6dk0	164	4	shown	show	VBN
cord-259738-yuqc6dk0	164	5	that	that	IN
cord-259738-yuqc6dk0	164	6	cellmediated	cellmediate	VBN
cord-259738-yuqc6dk0	164	7	immunity	immunity	NN
cord-259738-yuqc6dk0	164	8	to	to	IN
cord-259738-yuqc6dk0	164	9	IBV	IBV	NNP
cord-259738-yuqc6dk0	164	10	is	be	VBZ
cord-259738-yuqc6dk0	164	11	also	also	RB
cord-259738-yuqc6dk0	164	12	induced	induce	VBN
cord-259738-yuqc6dk0	164	13	and	and	CC
cord-259738-yuqc6dk0	164	14	is	be	VBZ
cord-259738-yuqc6dk0	164	15	believed	believe	VBN
cord-259738-yuqc6dk0	164	16	to	to	TO
cord-259738-yuqc6dk0	164	17	be	be	VB
cord-259738-yuqc6dk0	164	18	a	a	DT
cord-259738-yuqc6dk0	164	19	protective	protective	JJ
cord-259738-yuqc6dk0	164	20	mechanism	mechanism	NN
cord-259738-yuqc6dk0	164	21	in	in	IN
cord-259738-yuqc6dk0	164	22	IBV	IBV	NNP
cord-259738-yuqc6dk0	164	23	infection	infection	NN
cord-259738-yuqc6dk0	164	24	.	.	.
cord-259738-yuqc6dk0	165	1	CD8	CD8	NNP
cord-259738-yuqc6dk0	165	2	+	+	SYM
cord-259738-yuqc6dk0	165	3	CTL	CTL	NNP
cord-259738-yuqc6dk0	165	4	are	be	VBP
cord-259738-yuqc6dk0	165	5	critical	critical	JJ
cord-259738-yuqc6dk0	165	6	in	in	IN
cord-259738-yuqc6dk0	165	7	the	the	DT
cord-259738-yuqc6dk0	165	8	control	control	NN
cord-259738-yuqc6dk0	165	9	of	of	IN
cord-259738-yuqc6dk0	165	10	infectious	infectious	JJ
cord-259738-yuqc6dk0	165	11	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	165	12	in	in	IN
cord-259738-yuqc6dk0	165	13	poultry	poultry	NN
cord-259738-yuqc6dk0	165	14	Dhinakar	Dhinakar	NNP
cord-259738-yuqc6dk0	165	15	and	and	CC
cord-259738-yuqc6dk0	165	16	Jones	Jones	NNP
cord-259738-yuqc6dk0	165	17	,	,	,
cord-259738-yuqc6dk0	165	18	1997	1997	CD
cord-259738-yuqc6dk0	165	19	;	;	.
cord-259738-yuqc6dk0	166	1	Seo	Seo	NNP
cord-259738-yuqc6dk0	166	2	et	et	NNP
cord-259738-yuqc6dk0	166	3	al	al	NNP
cord-259738-yuqc6dk0	166	4	.	.	NNP
cord-259738-yuqc6dk0	166	5	,	,	,
cord-259738-yuqc6dk0	166	6	2000	2000	CD
cord-259738-yuqc6dk0	166	7	)	)	-RRB-
cord-259738-yuqc6dk0	166	8	.	.	.
cord-259738-yuqc6dk0	167	1	CD4	CD4	NNP
cord-259738-yuqc6dk0	167	2	+	+	CC
cord-259738-yuqc6dk0	167	3	T	t	NN
cord-259738-yuqc6dk0	167	4	-	-	HYPH
cord-259738-yuqc6dk0	167	5	cell	cell	NN
cord-259738-yuqc6dk0	167	6	responses	response	NNS
cord-259738-yuqc6dk0	167	7	may	may	MD
cord-259738-yuqc6dk0	167	8	increase	increase	VB
cord-259738-yuqc6dk0	167	9	the	the	DT
cord-259738-yuqc6dk0	167	10	proliferation	proliferation	NN
cord-259738-yuqc6dk0	167	11	,	,	,
cord-259738-yuqc6dk0	167	12	maturation	maturation	NN
cord-259738-yuqc6dk0	167	13	and	and	CC
cord-259738-yuqc6dk0	167	14	functional	functional	JJ
cord-259738-yuqc6dk0	167	15	activity	activity	NN
cord-259738-yuqc6dk0	167	16	of	of	IN
cord-259738-yuqc6dk0	167	17	CD8	CD8	NNP
cord-259738-yuqc6dk0	167	18	+	+	SYM
cord-259738-yuqc6dk0	167	19	CTL	CTL	NNP
cord-259738-yuqc6dk0	167	20	,	,	,
cord-259738-yuqc6dk0	167	21	providing	provide	VBG
cord-259738-yuqc6dk0	167	22	increased	increase	VBN
cord-259738-yuqc6dk0	167	23	help	help	NN
cord-259738-yuqc6dk0	167	24	for	for	IN
cord-259738-yuqc6dk0	167	25	B	b	NN
cord-259738-yuqc6dk0	167	26	-	-	HYPH
cord-259738-yuqc6dk0	167	27	cells	cell	NNS
cord-259738-yuqc6dk0	167	28	and	and	CC
cord-259738-yuqc6dk0	167	29	directly	directly	RB
cord-259738-yuqc6dk0	167	30	producing	produce	VBG
cord-259738-yuqc6dk0	167	31	antiviral	antiviral	JJ
cord-259738-yuqc6dk0	167	32	cytokines	cytokine	NNS
cord-259738-yuqc6dk0	167	33	.	.	.
cord-259738-yuqc6dk0	168	1	By	by	IN
cord-259738-yuqc6dk0	168	2	increasing	increase	VBG
cord-259738-yuqc6dk0	168	3	the	the	DT
cord-259738-yuqc6dk0	168	4	number	number	NN
cord-259738-yuqc6dk0	168	5	of	of	IN
cord-259738-yuqc6dk0	168	6	T	t	NN
cord-259738-yuqc6dk0	168	7	-	-	HYPH
cord-259738-yuqc6dk0	168	8	cells	cell	NNS
cord-259738-yuqc6dk0	168	9	at	at	IN
cord-259738-yuqc6dk0	168	10	the	the	DT
cord-259738-yuqc6dk0	168	11	same	same	JJ
cord-259738-yuqc6dk0	168	12	site	site	NN
cord-259738-yuqc6dk0	168	13	being	be	VBG
cord-259738-yuqc6dk0	168	14	able	able	JJ
cord-259738-yuqc6dk0	168	15	to	to	TO
cord-259738-yuqc6dk0	168	16	respond	respond	VB
cord-259738-yuqc6dk0	168	17	to	to	IN
cord-259738-yuqc6dk0	168	18	N	n	CD
cord-259738-yuqc6dk0	168	19	antigen	antigen	NN
cord-259738-yuqc6dk0	168	20	,	,	,
cord-259738-yuqc6dk0	168	21	there	there	EX
cord-259738-yuqc6dk0	168	22	might	may	MD
cord-259738-yuqc6dk0	168	23	have	have	VB
cord-259738-yuqc6dk0	168	24	been	be	VBN
cord-259738-yuqc6dk0	168	25	a	a	DT
cord-259738-yuqc6dk0	168	26	limiting	limit	VBG
cord-259738-yuqc6dk0	168	27	effect	effect	NN
cord-259738-yuqc6dk0	168	28	on	on	IN
cord-259738-yuqc6dk0	168	29	viral	viral	JJ
cord-259738-yuqc6dk0	168	30	replication	replication	NN
cord-259738-yuqc6dk0	168	31	leading	lead	VBG
cord-259738-yuqc6dk0	168	32	to	to	IN
cord-259738-yuqc6dk0	168	33	better	well	JJR
cord-259738-yuqc6dk0	168	34	protection	protection	NN
cord-259738-yuqc6dk0	168	35	.	.	.
cord-259738-yuqc6dk0	169	1	To	to	TO
cord-259738-yuqc6dk0	169	2	investigate	investigate	VB
cord-259738-yuqc6dk0	169	3	the	the	DT
cord-259738-yuqc6dk0	169	4	level	level	NN
cord-259738-yuqc6dk0	169	5	of	of	IN
cord-259738-yuqc6dk0	169	6	protection	protection	NN
cord-259738-yuqc6dk0	169	7	elicited	elicit	VBN
cord-259738-yuqc6dk0	169	8	by	by	IN
cord-259738-yuqc6dk0	169	9	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	169	10	-	-	HYPH
cord-259738-yuqc6dk0	169	11	N/	N/	NNP
cord-259738-yuqc6dk0	169	12	IL2	IL2	NNP
cord-259738-yuqc6dk0	169	13	,	,	,
cord-259738-yuqc6dk0	169	14	vaccinated	vaccinated	JJ
cord-259738-yuqc6dk0	169	15	chickens	chicken	NNS
cord-259738-yuqc6dk0	169	16	were	be	VBD
cord-259738-yuqc6dk0	169	17	challenged	challenge	VBN
cord-259738-yuqc6dk0	169	18	with	with	IN
cord-259738-yuqc6dk0	169	19	a	a	DT
cord-259738-yuqc6dk0	169	20	nephropathogenic	nephropathogenic	JJ
cord-259738-yuqc6dk0	169	21	strain	strain	NN
cord-259738-yuqc6dk0	169	22	of	of	IN
cord-259738-yuqc6dk0	169	23	IBV	IBV	NNP
cord-259738-yuqc6dk0	169	24	.	.	.
cord-259738-yuqc6dk0	170	1	Chickens	chicken	NNS
cord-259738-yuqc6dk0	170	2	that	that	WDT
cord-259738-yuqc6dk0	170	3	received	receive	VBD
cord-259738-yuqc6dk0	170	4	the	the	DT
cord-259738-yuqc6dk0	170	5	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	170	6	-	-	HYPH
cord-259738-yuqc6dk0	170	7	N	N	NNP
cord-259738-yuqc6dk0	170	8	/	/	SYM
cord-259738-yuqc6dk0	170	9	IL2	IL2	NNP
cord-259738-yuqc6dk0	170	10	plasmid	plasmid	NN
cord-259738-yuqc6dk0	170	11	DNA	DNA	NNP
cord-259738-yuqc6dk0	170	12	were	be	VBD
cord-259738-yuqc6dk0	170	13	better	well	RBR
cord-259738-yuqc6dk0	170	14	protected	protect	VBN
cord-259738-yuqc6dk0	170	15	than	than	IN
cord-259738-yuqc6dk0	170	16	that	that	DT
cord-259738-yuqc6dk0	170	17	administered	administer	VBN
cord-259738-yuqc6dk0	170	18	with	with	IN
cord-259738-yuqc6dk0	170	19	the	the	DT
cord-259738-yuqc6dk0	170	20	plasmid	plasmid	NN
cord-259738-yuqc6dk0	170	21	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	170	22	-	-	HYPH
cord-259738-yuqc6dk0	170	23	N.	N.	NNP
cord-259738-yuqc6dk0	170	24	Mortality	Mortality	NNP
cord-259738-yuqc6dk0	170	25	of	of	IN
cord-259738-yuqc6dk0	170	26	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	170	27	-	-	HYPH
cord-259738-yuqc6dk0	170	28	N	N	NNP
cord-259738-yuqc6dk0	170	29	/	/	SYM
cord-259738-yuqc6dk0	170	30	IL2	IL2	NNP
cord-259738-yuqc6dk0	170	31	group	group	NN
cord-259738-yuqc6dk0	170	32	was	be	VBD
cord-259738-yuqc6dk0	170	33	lower	low	JJR
cord-259738-yuqc6dk0	170	34	than	than	IN
cord-259738-yuqc6dk0	170	35	that	that	DT
cord-259738-yuqc6dk0	170	36	of	of	IN
cord-259738-yuqc6dk0	170	37	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	170	38	-	-	HYPH
cord-259738-yuqc6dk0	170	39	N	N	NNP
cord-259738-yuqc6dk0	170	40	group	group	NN
cord-259738-yuqc6dk0	170	41	.	.	.
cord-259738-yuqc6dk0	171	1	The	the	DT
cord-259738-yuqc6dk0	171	2	protection	protection	NN
cord-259738-yuqc6dk0	171	3	rate	rate	NN
cord-259738-yuqc6dk0	171	4	of	of	IN
cord-259738-yuqc6dk0	171	5	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	171	6	-	-	HYPH
cord-259738-yuqc6dk0	171	7	N	N	NNP
cord-259738-yuqc6dk0	171	8	/	/	SYM
cord-259738-yuqc6dk0	171	9	IL2	IL2	NNP
cord-259738-yuqc6dk0	171	10	group	group	NN
cord-259738-yuqc6dk0	171	11	was	be	VBD
cord-259738-yuqc6dk0	171	12	the	the	DT
cord-259738-yuqc6dk0	171	13	highest	high	JJS
cord-259738-yuqc6dk0	171	14	in	in	IN
cord-259738-yuqc6dk0	171	15	all	all	PDT
cord-259738-yuqc6dk0	171	16	the	the	DT
cord-259738-yuqc6dk0	171	17	vaccination	vaccination	NN
cord-259738-yuqc6dk0	171	18	groups	group	NNS
cord-259738-yuqc6dk0	171	19	,	,	,
cord-259738-yuqc6dk0	171	20	possibly	possibly	RB
cord-259738-yuqc6dk0	171	21	indicating	indicate	VBG
cord-259738-yuqc6dk0	171	22	protective	protective	JJ
cord-259738-yuqc6dk0	171	23	immunity	immunity	NN
cord-259738-yuqc6dk0	171	24	overcome	overcome	VBN
cord-259738-yuqc6dk0	171	25	by	by	IN
cord-259738-yuqc6dk0	171	26	virus	virus	NN
cord-259738-yuqc6dk0	171	27	aggressiveness	aggressiveness	NN
cord-259738-yuqc6dk0	171	28	.	.	.
cord-259738-yuqc6dk0	172	1	These	these	DT
cord-259738-yuqc6dk0	172	2	results	result	NNS
cord-259738-yuqc6dk0	172	3	suggested	suggest	VBD
cord-259738-yuqc6dk0	172	4	that	that	IN
cord-259738-yuqc6dk0	172	5	vaccination	vaccination	NN
cord-259738-yuqc6dk0	172	6	with	with	IN
cord-259738-yuqc6dk0	172	7	the	the	DT
cord-259738-yuqc6dk0	172	8	co	co	JJ
cord-259738-yuqc6dk0	172	9	-	-	JJ
cord-259738-yuqc6dk0	172	10	expression	expression	JJ
cord-259738-yuqc6dk0	172	11	plasmid	plasmid	NN
cord-259738-yuqc6dk0	172	12	of	of	IN
cord-259738-yuqc6dk0	172	13	N	N	NNP
cord-259738-yuqc6dk0	172	14	protein	protein	NN
cord-259738-yuqc6dk0	172	15	gene	gene	NN
cord-259738-yuqc6dk0	172	16	and	and	CC
cord-259738-yuqc6dk0	172	17	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	172	18	may	may	MD
cord-259738-yuqc6dk0	172	19	have	have	VB
cord-259738-yuqc6dk0	172	20	increased	increase	VBN
cord-259738-yuqc6dk0	172	21	the	the	DT
cord-259738-yuqc6dk0	172	22	protection	protection	NN
cord-259738-yuqc6dk0	172	23	rate	rate	NN
cord-259738-yuqc6dk0	172	24	against	against	IN
cord-259738-yuqc6dk0	172	25	challenge	challenge	NN
cord-259738-yuqc6dk0	172	26	.	.	.
cord-259738-yuqc6dk0	173	1	Results	result	NNS
cord-259738-yuqc6dk0	173	2	of	of	IN
cord-259738-yuqc6dk0	173	3	immune	immune	JJ
cord-259738-yuqc6dk0	173	4	response	response	NN
cord-259738-yuqc6dk0	173	5	and	and	CC
cord-259738-yuqc6dk0	173	6	viral	viral	JJ
cord-259738-yuqc6dk0	173	7	challenge	challenge	NN
cord-259738-yuqc6dk0	173	8	showed	show	VBD
cord-259738-yuqc6dk0	173	9	that	that	IN
cord-259738-yuqc6dk0	173	10	the	the	DT
cord-259738-yuqc6dk0	173	11	group	group	NN
cord-259738-yuqc6dk0	173	12	inoculated	inoculate	VBN
cord-259738-yuqc6dk0	173	13	with	with	IN
cord-259738-yuqc6dk0	173	14	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	173	15	-	-	HYPH
cord-259738-yuqc6dk0	173	16	N	N	NNP
cord-259738-yuqc6dk0	173	17	/	/	SYM
cord-259738-yuqc6dk0	173	18	IL2	IL2	NNP
cord-259738-yuqc6dk0	173	19	provided	provide	VBD
cord-259738-yuqc6dk0	173	20	stronger	strong	JJR
cord-259738-yuqc6dk0	173	21	immune	immune	JJ
cord-259738-yuqc6dk0	173	22	response	response	NN
cord-259738-yuqc6dk0	173	23	and	and	CC
cord-259738-yuqc6dk0	173	24	better	well	JJR
cord-259738-yuqc6dk0	173	25	protection	protection	NN
cord-259738-yuqc6dk0	173	26	rate	rate	NN
cord-259738-yuqc6dk0	173	27	than	than	IN
cord-259738-yuqc6dk0	173	28	pIRES	pIRES	NNP
cord-259738-yuqc6dk0	173	29	-	-	HYPH
cord-259738-yuqc6dk0	173	30	N	N	NNP
cord-259738-yuqc6dk0	173	31	vaccinated	vaccinate	VBD
cord-259738-yuqc6dk0	173	32	group	group	NN
cord-259738-yuqc6dk0	173	33	.	.	.
cord-259738-yuqc6dk0	174	1	This	this	DT
cord-259738-yuqc6dk0	174	2	indicated	indicate	VBD
cord-259738-yuqc6dk0	174	3	that	that	IN
cord-259738-yuqc6dk0	174	4	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	174	5	might	may	MD
cord-259738-yuqc6dk0	174	6	effectively	effectively	RB
cord-259738-yuqc6dk0	174	7	enhanced	enhance	VBN
cord-259738-yuqc6dk0	174	8	humoral	humoral	JJ
cord-259738-yuqc6dk0	174	9	-	-	HYPH
cord-259738-yuqc6dk0	174	10	mediated	mediate	VBN
cord-259738-yuqc6dk0	174	11	immune	immune	NN
cord-259738-yuqc6dk0	174	12	and	and	CC
cord-259738-yuqc6dk0	174	13	cell	cell	NN
cord-259738-yuqc6dk0	174	14	-	-	HYPH
cord-259738-yuqc6dk0	174	15	mediated	mediate	VBN
cord-259738-yuqc6dk0	174	16	immune	immune	JJ
cord-259738-yuqc6dk0	174	17	responses	response	NNS
cord-259738-yuqc6dk0	174	18	to	to	IN
cord-259738-yuqc6dk0	174	19	some	some	DT
cord-259738-yuqc6dk0	174	20	extent	extent	NN
cord-259738-yuqc6dk0	174	21	.	.	.
cord-259738-yuqc6dk0	175	1	This	this	DT
cord-259738-yuqc6dk0	175	2	was	be	VBD
cord-259738-yuqc6dk0	175	3	consistent	consistent	JJ
cord-259738-yuqc6dk0	175	4	with	with	IN
cord-259738-yuqc6dk0	175	5	the	the	DT
cord-259738-yuqc6dk0	175	6	result	result	NN
cord-259738-yuqc6dk0	175	7	of	of	IN
cord-259738-yuqc6dk0	175	8	previous	previous	JJ
cord-259738-yuqc6dk0	175	9	reports	report	NNS
cord-259738-yuqc6dk0	175	10	(	(	-LRB-
cord-259738-yuqc6dk0	175	11	Li	Li	NNP
cord-259738-yuqc6dk0	175	12	et	et	NNP
cord-259738-yuqc6dk0	175	13	al	al	NNP
cord-259738-yuqc6dk0	175	14	.	.	NNP
cord-259738-yuqc6dk0	175	15	,	,	,
cord-259738-yuqc6dk0	175	16	2006	2006	CD
cord-259738-yuqc6dk0	175	17	;	;	:
cord-259738-yuqc6dk0	175	18	Chow	Chow	NNP
cord-259738-yuqc6dk0	175	19	et	et	NNP
cord-259738-yuqc6dk0	175	20	al	al	NNP
cord-259738-yuqc6dk0	175	21	.	.	NNP
cord-259738-yuqc6dk0	175	22	,	,	,
cord-259738-yuqc6dk0	175	23	1997	1997	CD
cord-259738-yuqc6dk0	175	24	;	;	:
cord-259738-yuqc6dk0	175	25	Henke	Henke	NNP
cord-259738-yuqc6dk0	175	26	et	et	NNP
cord-259738-yuqc6dk0	175	27	al	al	NNP
cord-259738-yuqc6dk0	175	28	.	.	NNP
cord-259738-yuqc6dk0	175	29	,	,	,
cord-259738-yuqc6dk0	175	30	2004	2004	CD
cord-259738-yuqc6dk0	175	31	)	)	-RRB-
cord-259738-yuqc6dk0	175	32	.	.	.
cord-259738-yuqc6dk0	176	1	These	these	DT
cord-259738-yuqc6dk0	176	2	results	result	NNS
cord-259738-yuqc6dk0	176	3	demonstrated	demonstrate	VBD
cord-259738-yuqc6dk0	176	4	that	that	IN
cord-259738-yuqc6dk0	176	5	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	176	6	DNA	dna	NN
cord-259738-yuqc6dk0	176	7	vaccine	vaccine	NN
cord-259738-yuqc6dk0	176	8	is	be	VBZ
cord-259738-yuqc6dk0	176	9	an	an	DT
cord-259738-yuqc6dk0	176	10	effective	effective	JJ
cord-259738-yuqc6dk0	176	11	approach	approach	NN
cord-259738-yuqc6dk0	176	12	to	to	TO
cord-259738-yuqc6dk0	176	13	increase	increase	VB
cord-259738-yuqc6dk0	176	14	IBV	IBV	NNP
cord-259738-yuqc6dk0	176	15	DNA	DNA	NNP
cord-259738-yuqc6dk0	176	16	vaccine	vaccine	NN
cord-259738-yuqc6dk0	176	17	immunogenicity	immunogenicity	NN
cord-259738-yuqc6dk0	176	18	.	.	.
cord-259738-yuqc6dk0	177	1	Our	-PRON-	PRP$
cord-259738-yuqc6dk0	177	2	results	result	NNS
cord-259738-yuqc6dk0	177	3	showing	show	VBG
cord-259738-yuqc6dk0	177	4	the	the	DT
cord-259738-yuqc6dk0	177	5	induction	induction	NN
cord-259738-yuqc6dk0	177	6	of	of	IN
cord-259738-yuqc6dk0	177	7	both	both	DT
cord-259738-yuqc6dk0	177	8	antibody	antibody	NN
cord-259738-yuqc6dk0	177	9	and	and	CC
cord-259738-yuqc6dk0	177	10	T	t	NN
cord-259738-yuqc6dk0	177	11	-	-	HYPH
cord-259738-yuqc6dk0	177	12	cell	cell	NN
cord-259738-yuqc6dk0	177	13	responses	response	NNS
cord-259738-yuqc6dk0	177	14	against	against	IN
cord-259738-yuqc6dk0	177	15	the	the	DT
cord-259738-yuqc6dk0	177	16	IBV	IBV	NNP
cord-259738-yuqc6dk0	177	17	challenge	challenge	NN
cord-259738-yuqc6dk0	177	18	in	in	IN
cord-259738-yuqc6dk0	177	19	chickens	chicken	NNS
cord-259738-yuqc6dk0	177	20	demonstrate	demonstrate	VBP
cord-259738-yuqc6dk0	177	21	that	that	IN
cord-259738-yuqc6dk0	177	22	the	the	DT
cord-259738-yuqc6dk0	177	23	delivery	delivery	NN
cord-259738-yuqc6dk0	177	24	of	of	IN
cord-259738-yuqc6dk0	177	25	antigens	antigen	NNS
cord-259738-yuqc6dk0	177	26	and	and	CC
cord-259738-yuqc6dk0	177	27	cytokines	cytokine	NNS
cord-259738-yuqc6dk0	177	28	via	via	IN
cord-259738-yuqc6dk0	177	29	bicistronic	bicistronic	JJ
cord-259738-yuqc6dk0	177	30	vectors	vector	NNS
cord-259738-yuqc6dk0	177	31	is	be	VBZ
cord-259738-yuqc6dk0	177	32	feasible	feasible	JJ
cord-259738-yuqc6dk0	177	33	in	in	IN
cord-259738-yuqc6dk0	177	34	the	the	DT
cord-259738-yuqc6dk0	177	35	chicken	chicken	NN
cord-259738-yuqc6dk0	177	36	model	model	NN
cord-259738-yuqc6dk0	177	37	.	.	.
cord-259738-yuqc6dk0	178	1	Rational	rational	JJ
cord-259738-yuqc6dk0	178	2	design	design	NN
cord-259738-yuqc6dk0	178	3	of	of	IN
cord-259738-yuqc6dk0	178	4	gene	gene	NN
cord-259738-yuqc6dk0	178	5	-	-	HYPH
cord-259738-yuqc6dk0	178	6	based	base	VBN
cord-259738-yuqc6dk0	178	7	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	179	1	The	the	DT
cord-259738-yuqc6dk0	179	2	role	role	NN
cord-259738-yuqc6dk0	179	3	of	of	IN
cord-259738-yuqc6dk0	179	4	cytokine	cytokine	NN
cord-259738-yuqc6dk0	179	5	DNAs	dna	NNS
cord-259738-yuqc6dk0	179	6	as	as	IN
cord-259738-yuqc6dk0	179	7	vaccine	vaccine	NN
cord-259738-yuqc6dk0	179	8	adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	179	9	for	for	IN
cord-259738-yuqc6dk0	179	10	optimizing	optimize	VBG
cord-259738-yuqc6dk0	179	11	cellular	cellular	JJ
cord-259738-yuqc6dk0	179	12	immune	immune	JJ
cord-259738-yuqc6dk0	179	13	responses	response	NNS
cord-259738-yuqc6dk0	179	14	Development	development	NN
cord-259738-yuqc6dk0	179	15	and	and	CC
cord-259738-yuqc6dk0	179	16	use	use	NN
cord-259738-yuqc6dk0	179	17	of	of	IN
cord-259738-yuqc6dk0	179	18	the	the	DT
cord-259738-yuqc6dk0	179	19	H	H	NNP
cord-259738-yuqc6dk0	179	20	strain	strain	NN
cord-259738-yuqc6dk0	179	21	of	of	IN
cord-259738-yuqc6dk0	179	22	avian	avian	JJ
cord-259738-yuqc6dk0	179	23	infectious	infectious	JJ
cord-259738-yuqc6dk0	179	24	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	179	25	virus	virus	NN
cord-259738-yuqc6dk0	179	26	from	from	IN
cord-259738-yuqc6dk0	179	27	The	the	DT
cord-259738-yuqc6dk0	179	28	Netherlands	Netherlands	NNP
cord-259738-yuqc6dk0	179	29	as	as	IN
cord-259738-yuqc6dk0	179	30	a	a	DT
cord-259738-yuqc6dk0	179	31	vaccine	vaccine	NN
cord-259738-yuqc6dk0	179	32	:	:	:
cord-259738-yuqc6dk0	179	33	a	a	DT
cord-259738-yuqc6dk0	179	34	review	review	NN
cord-259738-yuqc6dk0	179	35	Induction	Induction	NNP
cord-259738-yuqc6dk0	179	36	of	of	IN
cord-259738-yuqc6dk0	179	37	anti	anti	JJ
cord-259738-yuqc6dk0	179	38	-	-	JJ
cord-259738-yuqc6dk0	179	39	viral	viral	JJ
cord-259738-yuqc6dk0	179	40	immune	immune	JJ
cord-259738-yuqc6dk0	179	41	responses	response	NNS
cord-259738-yuqc6dk0	179	42	by	by	IN
cord-259738-yuqc6dk0	179	43	immunization	immunization	NN
cord-259738-yuqc6dk0	179	44	with	with	IN
cord-259738-yuqc6dk0	180	1	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	180	2	-	-	HYPH
cord-259738-yuqc6dk0	180	3	DNA	dna	NN
cord-259738-yuqc6dk0	180	4	encoded	encode	VBN
cord-259738-yuqc6dk0	180	5	avian	avian	JJ
cord-259738-yuqc6dk0	180	6	coronavirus	coronavirus	NN
cord-259738-yuqc6dk0	180	7	nucleocapsid	nucleocapsid	NN
cord-259738-yuqc6dk0	180	8	protein	protein	NN
cord-259738-yuqc6dk0	180	9	Co	co	NN
cord-259738-yuqc6dk0	180	10	-	-	NN
cord-259738-yuqc6dk0	180	11	delivery	delivery	NN
cord-259738-yuqc6dk0	180	12	of	of	IN
cord-259738-yuqc6dk0	180	13	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	180	14	or	or	CC
cord-259738-yuqc6dk0	180	15	liposomes	liposome	NNS
cord-259738-yuqc6dk0	180	16	augment	augment	VB
cord-259738-yuqc6dk0	180	17	the	the	DT
cord-259738-yuqc6dk0	180	18	responses	response	NNS
cord-259738-yuqc6dk0	180	19	of	of	IN
cord-259738-yuqc6dk0	180	20	mice	mouse	NNS
cord-259738-yuqc6dk0	180	21	to	to	IN
cord-259738-yuqc6dk0	180	22	a	a	DT
cord-259738-yuqc6dk0	180	23	DNA	dna	NN
cord-259738-yuqc6dk0	180	24	vaccine	vaccine	NN
cord-259738-yuqc6dk0	180	25	for	for	IN
cord-259738-yuqc6dk0	180	26	pseudorabies	pseudorabies	NN
cord-259738-yuqc6dk0	180	27	virus	virus	NN
cord-259738-yuqc6dk0	180	28	IE80	IE80	NNP
cord-259738-yuqc6dk0	180	29	Enhancement	Enhancement	NNP
cord-259738-yuqc6dk0	180	30	of	of	IN
cord-259738-yuqc6dk0	180	31	human	human	JJ
cord-259738-yuqc6dk0	180	32	immunodeficiency	immunodeficiency	NN
cord-259738-yuqc6dk0	180	33	virus	virus	NN
cord-259738-yuqc6dk0	180	34	type	type	NN
cord-259738-yuqc6dk0	180	35	1-DNA	1-dna	CD
cord-259738-yuqc6dk0	180	36	vaccine	vaccine	NN
cord-259738-yuqc6dk0	180	37	potency	potency	NN
cord-259738-yuqc6dk0	180	38	through	through	IN
cord-259738-yuqc6dk0	180	39	incorporation	incorporation	NN
cord-259738-yuqc6dk0	180	40	of	of	IN
cord-259738-yuqc6dk0	180	41	T	T	NNP
cord-259738-yuqc6dk0	180	42	-	-	HYPH
cord-259738-yuqc6dk0	180	43	helper	helper	NN
cord-259738-yuqc6dk0	180	44	1	1	CD
cord-259738-yuqc6dk0	180	45	molecular	molecular	JJ
cord-259738-yuqc6dk0	180	46	adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	181	1	Severe	severe	JJ
cord-259738-yuqc6dk0	181	2	acute	acute	JJ
cord-259738-yuqc6dk0	181	3	respiratory	respiratory	JJ
cord-259738-yuqc6dk0	181	4	syndrome	syndrome	NN
cord-259738-yuqc6dk0	181	5	vaccine	vaccine	NN
cord-259738-yuqc6dk0	181	6	development	development	NN
cord-259738-yuqc6dk0	181	7	:	:	:
cord-259738-yuqc6dk0	181	8	experiences	experience	NNS
cord-259738-yuqc6dk0	181	9	of	of	IN
cord-259738-yuqc6dk0	181	10	vaccination	vaccination	NN
cord-259738-yuqc6dk0	181	11	against	against	IN
cord-259738-yuqc6dk0	181	12	avian	avian	JJ
cord-259738-yuqc6dk0	181	13	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	14	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	15	coronavirus	coronavirus	NN
cord-259738-yuqc6dk0	181	16	Infectious	infectious	JJ
cord-259738-yuqc6dk0	181	17	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	18	Improvement	improvement	NN
cord-259738-yuqc6dk0	181	19	of	of	IN
cord-259738-yuqc6dk0	181	20	hepatitis	hepatitis	NN
cord-259738-yuqc6dk0	181	21	B	B	NNP
cord-259738-yuqc6dk0	181	22	virus	virus	NN
cord-259738-yuqc6dk0	181	23	DNA	dna	NN
cord-259738-yuqc6dk0	181	24	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	181	25	by	by	IN
cord-259738-yuqc6dk0	181	26	plasmids	plasmid	NNS
cord-259738-yuqc6dk0	181	27	coexpressing	coexpresse	VBG
cord-259738-yuqc6dk0	181	28	hepatitis	hepatitis	NNP
cord-259738-yuqc6dk0	181	29	B	B	NNP
cord-259738-yuqc6dk0	181	30	surface	surface	NN
cord-259738-yuqc6dk0	181	31	antigen	antigen	NN
cord-259738-yuqc6dk0	181	32	and	and	CC
cord-259738-yuqc6dk0	181	33	interleukin-2	interleukin-2	IN
cord-259738-yuqc6dk0	181	34	Cytotoxic	Cytotoxic	NNP
cord-259738-yuqc6dk0	181	35	T	t	NN
cord-259738-yuqc6dk0	181	36	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	181	37	are	be	VBP
cord-259738-yuqc6dk0	181	38	critical	critical	JJ
cord-259738-yuqc6dk0	181	39	in	in	IN
cord-259738-yuqc6dk0	181	40	the	the	DT
cord-259738-yuqc6dk0	181	41	control	control	NN
cord-259738-yuqc6dk0	181	42	of	of	IN
cord-259738-yuqc6dk0	181	43	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	44	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	45	in	in	IN
cord-259738-yuqc6dk0	181	46	poultry	poultry	NN
cord-259738-yuqc6dk0	181	47	Infectious	infectious	JJ
cord-259738-yuqc6dk0	181	48	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	49	immunity	immunity	NN
cord-259738-yuqc6dk0	181	50	:	:	:
cord-259738-yuqc6dk0	181	51	its	-PRON-	PRP$
cord-259738-yuqc6dk0	181	52	study	study	NN
cord-259738-yuqc6dk0	181	53	in	in	IN
cord-259738-yuqc6dk0	181	54	chickens	chicken	NNS
cord-259738-yuqc6dk0	181	55	experimentally	experimentally	RB
cord-259738-yuqc6dk0	181	56	infected	infect	VBN
cord-259738-yuqc6dk0	181	57	with	with	IN
cord-259738-yuqc6dk0	181	58	mixtures	mixture	NNS
cord-259738-yuqc6dk0	181	59	of	of	IN
cord-259738-yuqc6dk0	181	60	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	61	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	62	virus	virus	NN
cord-259738-yuqc6dk0	181	63	and	and	CC
cord-259738-yuqc6dk0	181	64	Escherichia	escherichia	NN
cord-259738-yuqc6dk0	181	65	coli	coli	NNS
cord-259738-yuqc6dk0	181	66	Effect	effect	NN
cord-259738-yuqc6dk0	181	67	of	of	IN
cord-259738-yuqc6dk0	181	68	in	in	IN
cord-259738-yuqc6dk0	181	69	vivo	vivo	NNP
cord-259738-yuqc6dk0	181	70	bursectomy	bursectomy	NN
cord-259738-yuqc6dk0	181	71	on	on	IN
cord-259738-yuqc6dk0	181	72	the	the	DT
cord-259738-yuqc6dk0	181	73	course	course	NN
cord-259738-yuqc6dk0	181	74	of	of	IN
cord-259738-yuqc6dk0	181	75	an	an	DT
cord-259738-yuqc6dk0	181	76	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	77	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	78	virus	virus	NN
cord-259738-yuqc6dk0	181	79	infection	infection	NN
cord-259738-yuqc6dk0	181	80	in	in	IN
cord-259738-yuqc6dk0	181	81	line	line	NN
cord-259738-yuqc6dk0	181	82	C	C	NNP
cord-259738-yuqc6dk0	181	83	White	White	NNP
cord-259738-yuqc6dk0	181	84	Leghoen	Leghoen	NNP
cord-259738-yuqc6dk0	181	85	chickens	chicken	NNS
cord-259738-yuqc6dk0	181	86	Cross	Cross	NNP
cord-259738-yuqc6dk0	181	87	-	-	JJ
cord-259738-yuqc6dk0	181	88	reactive	reactive	JJ
cord-259738-yuqc6dk0	181	89	cellular	cellular	JJ
cord-259738-yuqc6dk0	181	90	immnue	immnue	NN
cord-259738-yuqc6dk0	181	91	responses	response	NNS
cord-259738-yuqc6dk0	181	92	in	in	IN
cord-259738-yuqc6dk0	181	93	chickens	chicken	NNS
cord-259738-yuqc6dk0	181	94	vaccinated	vaccinate	VBN
cord-259738-yuqc6dk0	181	95	with	with	IN
cord-259738-yuqc6dk0	181	96	live	live	JJ
cord-259738-yuqc6dk0	181	97	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	98	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	99	virus	virus	NN
cord-259738-yuqc6dk0	181	100	vaccine	vaccine	NN
cord-259738-yuqc6dk0	181	101	Partial	partial	JJ
cord-259738-yuqc6dk0	181	102	protection	protection	NN
cord-259738-yuqc6dk0	181	103	against	against	IN
cord-259738-yuqc6dk0	181	104	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	105	bursal	bursal	NN
cord-259738-yuqc6dk0	181	106	disease	disease	NN
cord-259738-yuqc6dk0	181	107	virus	virus	NN
cord-259738-yuqc6dk0	181	108	through	through	IN
cord-259738-yuqc6dk0	181	109	DNA	dna	NN
cord-259738-yuqc6dk0	181	110	-	-	HYPH
cord-259738-yuqc6dk0	181	111	mediated	mediate	VBN
cord-259738-yuqc6dk0	181	112	vaccination	vaccination	NN
cord-259738-yuqc6dk0	181	113	with	with	IN
cord-259738-yuqc6dk0	181	114	the	the	DT
cord-259738-yuqc6dk0	181	115	VP2	VP2	NNP
cord-259738-yuqc6dk0	181	116	capsid	capsid	NN
cord-259738-yuqc6dk0	181	117	protein	protein	NN
cord-259738-yuqc6dk0	181	118	and	and	CC
cord-259738-yuqc6dk0	181	119	chicken	chicken	NN
cord-259738-yuqc6dk0	181	120	IL-2	IL-2	NNP
cord-259738-yuqc6dk0	181	121	genes	gene	VBZ
cord-259738-yuqc6dk0	181	122	Infectious	infectious	JJ
cord-259738-yuqc6dk0	181	123	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	124	virus	virus	NN
cord-259738-yuqc6dk0	181	125	antibodies	antibody	NNS
cord-259738-yuqc6dk0	181	126	in	in	IN
cord-259738-yuqc6dk0	181	127	tears	tear	NNS
cord-259738-yuqc6dk0	181	128	and	and	CC
cord-259738-yuqc6dk0	181	129	their	-PRON-	PRP$
cord-259738-yuqc6dk0	181	130	relationship	relationship	NN
cord-259738-yuqc6dk0	181	131	to	to	IN
cord-259738-yuqc6dk0	181	132	immunity	immunity	NN
cord-259738-yuqc6dk0	181	133	Comparison	comparison	NN
cord-259738-yuqc6dk0	181	134	of	of	IN
cord-259738-yuqc6dk0	181	135	duration	duration	NN
cord-259738-yuqc6dk0	181	136	of	of	IN
cord-259738-yuqc6dk0	181	137	immunity	immunity	NN
cord-259738-yuqc6dk0	181	138	in	in	IN
cord-259738-yuqc6dk0	181	139	chickens	chicken	NNS
cord-259738-yuqc6dk0	181	140	infected	infect	VBN
cord-259738-yuqc6dk0	181	141	with	with	IN
cord-259738-yuqc6dk0	181	142	a	a	DT
cord-259738-yuqc6dk0	181	143	live	live	JJ
cord-259738-yuqc6dk0	181	144	infectious	infectious	JJ
cord-259738-yuqc6dk0	181	145	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	181	146	vaccine	vaccine	NN
cord-259738-yuqc6dk0	181	147	by	by	IN
cord-259738-yuqc6dk0	181	148	three	three	CD
cord-259738-yuqc6dk0	181	149	different	different	JJ
cord-259738-yuqc6dk0	181	150	routes	route	NNS
cord-259738-yuqc6dk0	181	151	DNA	dna	VBP
cord-259738-yuqc6dk0	181	152	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	181	153	:	:	:
cord-259738-yuqc6dk0	181	154	immunology	immunology	NN
cord-259738-yuqc6dk0	181	155	,	,	,
cord-259738-yuqc6dk0	181	156	application	application	NN
cord-259738-yuqc6dk0	181	157	,	,	,
cord-259738-yuqc6dk0	181	158	and	and	CC
cord-259738-yuqc6dk0	181	159	optimization	optimization	NN
cord-259738-yuqc6dk0	181	160	Co	co	NN
cord-259738-yuqc6dk0	181	161	-	-	NN
cord-259738-yuqc6dk0	181	162	expression	expression	NN
cord-259738-yuqc6dk0	181	163	of	of	IN
cord-259738-yuqc6dk0	181	164	interleukin-2	interleukin-2	IN
cord-259738-yuqc6dk0	181	165	to	to	TO
cord-259738-yuqc6dk0	181	166	increase	increase	VB
cord-259738-yuqc6dk0	181	167	the	the	DT
cord-259738-yuqc6dk0	181	168	efficacy	efficacy	NN
cord-259738-yuqc6dk0	181	169	of	of	IN
cord-259738-yuqc6dk0	181	170	DNA	dna	NN
cord-259738-yuqc6dk0	181	171	vaccine	vaccine	NN
cord-259738-yuqc6dk0	181	172	-	-	HYPH
cord-259738-yuqc6dk0	181	173	mediated	mediate	VBN
cord-259738-yuqc6dk0	181	174	protection	protection	NN
cord-259738-yuqc6dk0	181	175	in	in	IN
cord-259738-yuqc6dk0	181	176	coxsackievirus	coxsackievirus	NNP
cord-259738-yuqc6dk0	181	177	B3-infected	b3-infecte	VBD
cord-259738-yuqc6dk0	181	178	mice	mouse	NNS
cord-259738-yuqc6dk0	181	179	DNA	dna	NN
cord-259738-yuqc6dk0	181	180	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	181	181	for	for	IN
cord-259738-yuqc6dk0	181	182	parasitic	parasitic	JJ
cord-259738-yuqc6dk0	181	183	infections	infection	NNS
cord-259738-yuqc6dk0	181	184	Coexpression	Coexpression	NNP
cord-259738-yuqc6dk0	181	185	of	of	IN
cord-259738-yuqc6dk0	181	186	interleukin-2	interleukin-2	NNP
cord-259738-yuqc6dk0	181	187	enhances	enhance	VBZ
cord-259738-yuqc6dk0	181	188	the	the	DT
cord-259738-yuqc6dk0	181	189	immunization	immunization	NN
cord-259738-yuqc6dk0	181	190	effect	effect	NN
cord-259738-yuqc6dk0	181	191	of	of	IN
cord-259738-yuqc6dk0	181	192	a	a	DT
cord-259738-yuqc6dk0	181	193	DNA	dna	NN
cord-259738-yuqc6dk0	181	194	vaccine	vaccine	NN
cord-259738-yuqc6dk0	181	195	expressing	express	VBG
cord-259738-yuqc6dk0	181	196	herpes	herpes	NNP
cord-259738-yuqc6dk0	181	197	simplex	simplex	NNP
cord-259738-yuqc6dk0	181	198	1	1	CD
cord-259738-yuqc6dk0	181	199	glycoprotein	glycoprotein	NN
cord-259738-yuqc6dk0	181	200	D	d	NN
cord-259738-yuqc6dk0	181	201	DNA	dna	NN
cord-259738-yuqc6dk0	181	202	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	181	203	for	for	IN
cord-259738-yuqc6dk0	181	204	aquacultured	aquacultured	JJ
cord-259738-yuqc6dk0	181	205	fish	fish	NN
cord-259738-yuqc6dk0	182	1	Selective	selective	JJ
cord-259738-yuqc6dk0	182	2	in	in	IN
cord-259738-yuqc6dk0	182	3	vitro	vitro	FW
cord-259738-yuqc6dk0	182	4	growth	growth	NN
cord-259738-yuqc6dk0	182	5	of	of	IN
cord-259738-yuqc6dk0	182	6	T	t	NN
cord-259738-yuqc6dk0	182	7	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	182	8	from	from	IN
cord-259738-yuqc6dk0	182	9	normal	normal	JJ
cord-259738-yuqc6dk0	182	10	human	human	JJ
cord-259738-yuqc6dk0	182	11	bone	bone	NN
cord-259738-yuqc6dk0	182	12	marrows	marrow	VBZ
cord-259738-yuqc6dk0	182	13	DNA	DNA	NNP
cord-259738-yuqc6dk0	182	14	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	182	15	:	:	:
cord-259738-yuqc6dk0	182	16	back	back	RB
cord-259738-yuqc6dk0	182	17	in	in	IN
cord-259738-yuqc6dk0	182	18	the	the	DT
cord-259738-yuqc6dk0	182	19	saddle	saddle	NN
cord-259738-yuqc6dk0	182	20	again	again	RB
cord-259738-yuqc6dk0	182	21	?	?	.
cord-259738-yuqc6dk0	183	1	Lack	lack	NN
cord-259738-yuqc6dk0	183	2	of	of	IN
cord-259738-yuqc6dk0	183	3	correlation	correlation	NN
cord-259738-yuqc6dk0	183	4	between	between	IN
cord-259738-yuqc6dk0	183	5	infectivity	infectivity	NN
cord-259738-yuqc6dk0	183	6	,	,	,
cord-259738-yuqc6dk0	183	7	serologic	serologic	NN
cord-259738-yuqc6dk0	183	8	response	response	NN
cord-259738-yuqc6dk0	183	9	and	and	CC
cord-259738-yuqc6dk0	183	10	challenge	challenge	NN
cord-259738-yuqc6dk0	183	11	results	result	NNS
cord-259738-yuqc6dk0	183	12	in	in	IN
cord-259738-yuqc6dk0	183	13	immunization	immunization	NN
cord-259738-yuqc6dk0	183	14	with	with	IN
cord-259738-yuqc6dk0	183	15	an	an	DT
cord-259738-yuqc6dk0	183	16	avian	avian	JJ
cord-259738-yuqc6dk0	183	17	infectious	infectious	JJ
cord-259738-yuqc6dk0	183	18	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	183	19	vaccine	vaccine	NN
cord-259738-yuqc6dk0	183	20	DNA	dna	NN
cord-259738-yuqc6dk0	183	21	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	183	22	Positive	positive	JJ
cord-259738-yuqc6dk0	183	23	inductive	inductive	JJ
cord-259738-yuqc6dk0	183	24	effect	effect	NN
cord-259738-yuqc6dk0	183	25	of	of	IN
cord-259738-yuqc6dk0	183	26	IL-2	il-2	NN
cord-259738-yuqc6dk0	183	27	on	on	IN
cord-259738-yuqc6dk0	183	28	virus	virus	NN
cord-259738-yuqc6dk0	183	29	-	-	HYPH
cord-259738-yuqc6dk0	183	30	specific	specific	JJ
cord-259738-yuqc6dk0	183	31	cellular	cellular	JJ
cord-259738-yuqc6dk0	183	32	responses	response	NNS
cord-259738-yuqc6dk0	183	33	elicited	elicit	VBN
cord-259738-yuqc6dk0	183	34	by	by	IN
cord-259738-yuqc6dk0	183	35	a	a	DT
cord-259738-yuqc6dk0	183	36	PRRSV	PRRSV	NNP
cord-259738-yuqc6dk0	183	37	-	-	HYPH
cord-259738-yuqc6dk0	183	38	ORF7	ORF7	NNP
cord-259738-yuqc6dk0	183	39	DNA	dna	NN
cord-259738-yuqc6dk0	183	40	vaccine	vaccine	NN
cord-259738-yuqc6dk0	183	41	in	in	IN
cord-259738-yuqc6dk0	183	42	swine	swine	NN
cord-259738-yuqc6dk0	183	43	Molecular	Molecular	NNP
cord-259738-yuqc6dk0	183	44	Cloning	Cloning	NNP
cord-259738-yuqc6dk0	183	45	-	-	HYPH
cord-259738-yuqc6dk0	183	46	A	A	NNP
cord-259738-yuqc6dk0	183	47	Laboratory	Laboratory	NNP
cord-259738-yuqc6dk0	183	48	Manual	Manual	NNP
cord-259738-yuqc6dk0	183	49	Genetic	genetic	JJ
cord-259738-yuqc6dk0	183	50	adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	183	51	for	for	IN
cord-259738-yuqc6dk0	183	52	DNA	dna	NN
cord-259738-yuqc6dk0	183	53	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	183	54	Location	Location	NNP
cord-259738-yuqc6dk0	183	55	of	of	IN
cord-259738-yuqc6dk0	183	56	linear	linear	JJ
cord-259738-yuqc6dk0	183	57	B	b	NN
cord-259738-yuqc6dk0	183	58	-	-	HYPH
cord-259738-yuqc6dk0	183	59	cell	cell	NN
cord-259738-yuqc6dk0	183	60	epitopes	epitope	NNS
cord-259738-yuqc6dk0	183	61	on	on	IN
cord-259738-yuqc6dk0	183	62	infectious	infectious	JJ
cord-259738-yuqc6dk0	183	63	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	183	64	virus	virus	NN
cord-259738-yuqc6dk0	183	65	nucleocapsid	nucleocapsid	NN
cord-259738-yuqc6dk0	183	66	protein	protein	NN
cord-259738-yuqc6dk0	183	67	Specific	specific	JJ
cord-259738-yuqc6dk0	183	68	cytotoxic	cytotoxic	JJ
cord-259738-yuqc6dk0	183	69	T	t	NN
cord-259738-yuqc6dk0	183	70	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	183	71	are	be	VBP
cord-259738-yuqc6dk0	183	72	involved	involve	VBN
cord-259738-yuqc6dk0	183	73	in	in	IN
cord-259738-yuqc6dk0	183	74	in	in	IN
cord-259738-yuqc6dk0	183	75	vivo	vivo	NN
cord-259738-yuqc6dk0	183	76	clearance	clearance	NN
cord-259738-yuqc6dk0	183	77	of	of	IN
cord-259738-yuqc6dk0	183	78	infectious	infectious	JJ
cord-259738-yuqc6dk0	183	79	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	183	80	virus	virus	NN
cord-259738-yuqc6dk0	184	1	The	the	DT
cord-259738-yuqc6dk0	184	2	carboxylterminal	carboxylterminal	JJ
cord-259738-yuqc6dk0	184	3	120-residue	120-residue	CD
cord-259738-yuqc6dk0	184	4	polypeptide	polypeptide	NN
cord-259738-yuqc6dk0	184	5	of	of	IN
cord-259738-yuqc6dk0	184	6	infectious	infectious	JJ
cord-259738-yuqc6dk0	184	7	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	184	8	virus	virus	NN
cord-259738-yuqc6dk0	184	9	nucleocapsid	nucleocapsid	NN
cord-259738-yuqc6dk0	184	10	induces	induce	VBZ
cord-259738-yuqc6dk0	184	11	cytotoxic	cytotoxic	JJ
cord-259738-yuqc6dk0	184	12	T	t	NN
cord-259738-yuqc6dk0	184	13	lymphocytes	lymphocyte	NNS
cord-259738-yuqc6dk0	184	14	and	and	CC
cord-259738-yuqc6dk0	184	15	protects	protect	VBZ
cord-259738-yuqc6dk0	184	16	chickens	chicken	NNS
cord-259738-yuqc6dk0	184	17	from	from	IN
cord-259738-yuqc6dk0	184	18	acute	acute	JJ
cord-259738-yuqc6dk0	184	19	infection	infection	NN
cord-259738-yuqc6dk0	184	20	Adoptive	adoptive	JJ
cord-259738-yuqc6dk0	184	21	transfer	transfer	NN
cord-259738-yuqc6dk0	184	22	of	of	IN
cord-259738-yuqc6dk0	184	23	infectious	infectious	JJ
cord-259738-yuqc6dk0	184	24	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	184	25	virus	virus	NN
cord-259738-yuqc6dk0	185	1	primed	prime	VBN
cord-259738-yuqc6dk0	185	2	alpha	alpha	NN
cord-259738-yuqc6dk0	185	3	beta	beta	NN
cord-259738-yuqc6dk0	185	4	T	T	NNP
cord-259738-yuqc6dk0	185	5	cells	cell	NNS
cord-259738-yuqc6dk0	185	6	bearing	bear	VBG
cord-259738-yuqc6dk0	185	7	CD8	CD8	NNP
cord-259738-yuqc6dk0	185	8	antigen	antigen	NNP
cord-259738-yuqc6dk0	185	9	protects	protect	VBZ
cord-259738-yuqc6dk0	185	10	chicks	chick	NNS
cord-259738-yuqc6dk0	185	11	from	from	IN
cord-259738-yuqc6dk0	185	12	acute	acute	JJ
cord-259738-yuqc6dk0	185	13	infection	infection	NN
cord-259738-yuqc6dk0	185	14	DNA	dna	NN
cord-259738-yuqc6dk0	185	15	vaccines	vaccine	NNS
cord-259738-yuqc6dk0	185	16	and	and	CC
cord-259738-yuqc6dk0	185	17	adjuvants	adjuvant	NNS
cord-259738-yuqc6dk0	185	18	DNA	dna	NN
cord-259738-yuqc6dk0	185	19	vaccine	vaccine	NN
cord-259738-yuqc6dk0	185	20	for	for	IN
cord-259738-yuqc6dk0	185	21	bacterial	bacterial	JJ
cord-259738-yuqc6dk0	185	22	infections	infection	NNS
cord-259738-yuqc6dk0	185	23	Phase	phase	NN
cord-259738-yuqc6dk0	186	1	I	-PRON-	PRP
cord-259738-yuqc6dk0	186	2	safety	safety	VBP
cord-259738-yuqc6dk0	186	3	and	and	CC
cord-259738-yuqc6dk0	186	4	immune	immune	JJ
cord-259738-yuqc6dk0	186	5	response	response	NN
cord-259738-yuqc6dk0	186	6	studies	study	NNS
cord-259738-yuqc6dk0	186	7	of	of	IN
cord-259738-yuqc6dk0	186	8	a	a	DT
cord-259738-yuqc6dk0	186	9	DNA	dna	NN
cord-259738-yuqc6dk0	186	10	vaccine	vaccine	NN
cord-259738-yuqc6dk0	187	1	encoding	encode	VBG
cord-259738-yuqc6dk0	187	2	hepatitis	hepatitis	NNP
cord-259738-yuqc6dk0	187	3	B	B	NNP
cord-259738-yuqc6dk0	187	4	surface	surface	NN
cord-259738-yuqc6dk0	187	5	antigen	antigen	NN
cord-259738-yuqc6dk0	187	6	delivered	deliver	VBN
cord-259738-yuqc6dk0	187	7	by	by	IN
cord-259738-yuqc6dk0	187	8	a	a	DT
cord-259738-yuqc6dk0	187	9	gene	gene	NN
cord-259738-yuqc6dk0	187	10	delivery	delivery	NN
cord-259738-yuqc6dk0	187	11	device	device	NN
cord-259738-yuqc6dk0	187	12	Systemic	systemic	JJ
cord-259738-yuqc6dk0	187	13	and	and	CC
cord-259738-yuqc6dk0	187	14	local	local	JJ
cord-259738-yuqc6dk0	187	15	antibody	antibody	NN
cord-259738-yuqc6dk0	187	16	responses	response	NNS
cord-259738-yuqc6dk0	187	17	to	to	IN
cord-259738-yuqc6dk0	187	18	infectious	infectious	JJ
cord-259738-yuqc6dk0	187	19	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	187	20	virus	virus	NN
cord-259738-yuqc6dk0	187	21	in	in	IN
cord-259738-yuqc6dk0	187	22	chickens	chicken	NNS
cord-259738-yuqc6dk0	187	23	inoculated	inoculate	VBN
cord-259738-yuqc6dk0	187	24	with	with	IN
cord-259738-yuqc6dk0	187	25	infectious	infectious	JJ
cord-259738-yuqc6dk0	187	26	bursal	bursal	NN
cord-259738-yuqc6dk0	187	27	disease	disease	NN
cord-259738-yuqc6dk0	187	28	virus	virus	NN
cord-259738-yuqc6dk0	187	29	and	and	CC
cord-259738-yuqc6dk0	187	30	control	control	NN
cord-259738-yuqc6dk0	187	31	chickens	chicken	NNS
cord-259738-yuqc6dk0	188	1	Avian	avian	JJ
cord-259738-yuqc6dk0	188	2	infectious	infectious	JJ
cord-259738-yuqc6dk0	188	3	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	188	4	:	:	:
cord-259738-yuqc6dk0	188	5	specific	specific	JJ
cord-259738-yuqc6dk0	188	6	lachrymal	lachrymal	NNP
cord-259738-yuqc6dk0	188	7	IgA	IgA	NNP
cord-259738-yuqc6dk0	188	8	level	level	NN
cord-259738-yuqc6dk0	188	9	and	and	CC
cord-259738-yuqc6dk0	188	10	resistance	resistance	NN
cord-259738-yuqc6dk0	188	11	against	against	IN
cord-259738-yuqc6dk0	188	12	challenge	challenge	NNP
cord-259738-yuqc6dk0	188	13	Study	Study	NNP
cord-259738-yuqc6dk0	188	14	of	of	IN
cord-259738-yuqc6dk0	188	15	protection	protection	NN
cord-259738-yuqc6dk0	188	16	by	by	IN
cord-259738-yuqc6dk0	188	17	recombinant	recombinant	JJ
cord-259738-yuqc6dk0	188	18	fowl	fowl	NNP
cord-259738-yuqc6dk0	188	19	poxvirus	poxvirus	NNP
cord-259738-yuqc6dk0	188	20	expressing	express	VBG
cord-259738-yuqc6dk0	188	21	C	C	NNP
cord-259738-yuqc6dk0	188	22	-	-	HYPH
cord-259738-yuqc6dk0	188	23	terminal	terminal	NN
cord-259738-yuqc6dk0	188	24	nucleocapsid	nucleocapsid	NNP
cord-259738-yuqc6dk0	188	25	protein	protein	NN
cord-259738-yuqc6dk0	188	26	of	of	IN
cord-259738-yuqc6dk0	188	27	infectious	infectious	JJ
cord-259738-yuqc6dk0	188	28	bronchitis	bronchitis	NN
cord-259738-yuqc6dk0	188	29	virus	virus	NN
cord-259738-yuqc6dk0	188	30	against	against	IN
cord-259738-yuqc6dk0	188	31	challenge	challenge	NN
cord-259738-yuqc6dk0	188	32	This	this	DT
cord-259738-yuqc6dk0	188	33	work	work	NN
cord-259738-yuqc6dk0	188	34	was	be	VBD
cord-259738-yuqc6dk0	188	35	supported	support	VBN
cord-259738-yuqc6dk0	188	36	by	by	IN
cord-259738-yuqc6dk0	188	37	the	the	DT
cord-259738-yuqc6dk0	188	38	foundation	foundation	NN
cord-259738-yuqc6dk0	188	39	of	of	IN
cord-259738-yuqc6dk0	188	40	Chinese	Chinese	NNP
cord-259738-yuqc6dk0	188	41	National	National	NNP
cord-259738-yuqc6dk0	188	42	Programs	Programs	NNPS
cord-259738-yuqc6dk0	188	43	for	for	IN
cord-259738-yuqc6dk0	188	44	High	High	NNP
cord-259738-yuqc6dk0	188	45	Technology	Technology	NNP
cord-259738-yuqc6dk0	188	46	Research	Research	NNP
cord-259738-yuqc6dk0	188	47	and	and	CC
cord-259738-yuqc6dk0	188	48	Development	Development	NNP
cord-259738-yuqc6dk0	188	49	(	(	-LRB-
cord-259738-yuqc6dk0	188	50	project	project	NN
cord-259738-yuqc6dk0	188	51	number	number	NN
cord-259738-yuqc6dk0	188	52	2006AA10A205	2006AA10A205	NNP
cord-259738-yuqc6dk0	188	53	)	)	-RRB-
cord-259738-yuqc6dk0	188	54	.	.	.