id	sid	tid	token	lemma	pos
cord-021063-4y8m33ea	1	1	key	key	NN
cord-021063-4y8m33ea	1	2	:	:	:
cord-021063-4y8m33ea	2	1	cord-021063	cord-021063	NNP
cord-021063-4y8m33ea	2	2	-	-	HYPH
cord-021063-4y8m33ea	2	3	4y8m33ea	4y8m33ea	CD
cord-021063-4y8m33ea	2	4	authors	author	NNS
cord-021063-4y8m33ea	2	5	:	:	:
cord-021063-4y8m33ea	2	6	Hug	hug	VB
cord-021063-4y8m33ea	2	7	,	,	,
cord-021063-4y8m33ea	2	8	Peter	Peter	NNP
cord-021063-4y8m33ea	2	9	;	;	:
cord-021063-4y8m33ea	2	10	Sleight	Sleight	NNP
cord-021063-4y8m33ea	2	11	,	,	,
cord-021063-4y8m33ea	3	1	Richard	Richard	NNP
cord-021063-4y8m33ea	3	2	G.	G.	NNP
cord-021063-4y8m33ea	3	3	title	title	NN
cord-021063-4y8m33ea	3	4	:	:	:
cord-021063-4y8m33ea	3	5	Chapter	chapter	NN
cord-021063-4y8m33ea	3	6	18	18	CD
cord-021063-4y8m33ea	4	1	The	the	DT
cord-021063-4y8m33ea	4	2	advantages	advantage	NNS
cord-021063-4y8m33ea	4	3	of	of	IN
cord-021063-4y8m33ea	4	4	liposome	liposome	NN
cord-021063-4y8m33ea	4	5	-	-	HYPH
cord-021063-4y8m33ea	4	6	based	base	VBN
cord-021063-4y8m33ea	4	7	gene	gene	NN
cord-021063-4y8m33ea	4	8	therapy	therapy	NN
cord-021063-4y8m33ea	4	9	:	:	:
cord-021063-4y8m33ea	4	10	A	a	DT
cord-021063-4y8m33ea	4	11	comparison	comparison	NN
cord-021063-4y8m33ea	4	12	of	of	IN
cord-021063-4y8m33ea	4	13	viral	viral	JJ
cord-021063-4y8m33ea	4	14	versus	versus	IN
cord-021063-4y8m33ea	4	15	liposome	liposome	NN
cord-021063-4y8m33ea	4	16	-	-	HYPH
cord-021063-4y8m33ea	4	17	based	base	VBN
cord-021063-4y8m33ea	4	18	gene	gene	NN
cord-021063-4y8m33ea	4	19	delivery	delivery	NN
cord-021063-4y8m33ea	4	20	date	date	NN
cord-021063-4y8m33ea	4	21	:	:	:
cord-021063-4y8m33ea	4	22	2007	2007	CD
cord-021063-4y8m33ea	4	23	-	-	SYM
cord-021063-4y8m33ea	4	24	09	09	CD
cord-021063-4y8m33ea	4	25	-	-	HYPH
cord-021063-4y8m33ea	4	26	02	02	CD
cord-021063-4y8m33ea	4	27	journal	journal	NN
cord-021063-4y8m33ea	4	28	:	:	:
cord-021063-4y8m33ea	4	29	nan	nan	NNP
cord-021063-4y8m33ea	4	30	DOI	DOI	NNP
cord-021063-4y8m33ea	4	31	:	:	:
cord-021063-4y8m33ea	4	32	10.1016	10.1016	CD
cord-021063-4y8m33ea	4	33	/	/	SYM
cord-021063-4y8m33ea	4	34	s1569	s1569	CD
cord-021063-4y8m33ea	4	35	-	-	HYPH
cord-021063-4y8m33ea	4	36	2582(97)80043	2582(97)80043	CD
cord-021063-4y8m33ea	4	37	-	-	HYPH
cord-021063-4y8m33ea	4	38	8	8	CD
cord-021063-4y8m33ea	4	39	sha	sha	NN
cord-021063-4y8m33ea	4	40	:	:	:
cord-021063-4y8m33ea	4	41	a347a23ce4452adcb772163e22fa377854605ad8	a347a23ce4452adcb772163e22fa377854605ad8	XX
cord-021063-4y8m33ea	4	42	doc_id	doc_id	CD
cord-021063-4y8m33ea	4	43	:	:	:
cord-021063-4y8m33ea	4	44	21063	21063	CD
cord-021063-4y8m33ea	4	45	cord_uid	cord_uid	NNS
cord-021063-4y8m33ea	4	46	:	:	:
cord-021063-4y8m33ea	5	1	4y8m33ea	4y8m33ea	LS
cord-021063-4y8m33ea	6	1	Viruses	virus	NNS
cord-021063-4y8m33ea	6	2	have	have	VBP
cord-021063-4y8m33ea	6	3	evolved	evolve	VBN
cord-021063-4y8m33ea	6	4	in	in	IN
cord-021063-4y8m33ea	6	5	such	such	PDT
cord-021063-4y8m33ea	6	6	a	a	DT
cord-021063-4y8m33ea	6	7	way	way	NN
cord-021063-4y8m33ea	6	8	they	-PRON-	PRP
cord-021063-4y8m33ea	6	9	are	be	VBP
cord-021063-4y8m33ea	6	10	able	able	JJ
cord-021063-4y8m33ea	6	11	to	to	TO
cord-021063-4y8m33ea	6	12	efficiently	efficiently	RB
cord-021063-4y8m33ea	6	13	introduce	introduce	VB
cord-021063-4y8m33ea	6	14	and	and	CC
cord-021063-4y8m33ea	6	15	express	express	VB
cord-021063-4y8m33ea	6	16	exogenous	exogenous	JJ
cord-021063-4y8m33ea	6	17	genes	gene	NNS
cord-021063-4y8m33ea	6	18	in	in	IN
cord-021063-4y8m33ea	6	19	eukaryotic	eukaryotic	JJ
cord-021063-4y8m33ea	6	20	cells	cell	NNS
cord-021063-4y8m33ea	6	21	.	.	.
cord-021063-4y8m33ea	7	1	Most	Most	JJS
cord-021063-4y8m33ea	7	2	viruses	virus	NNS
cord-021063-4y8m33ea	7	3	need	need	VBP
cord-021063-4y8m33ea	7	4	to	to	TO
cord-021063-4y8m33ea	7	5	maintain	maintain	VB
cord-021063-4y8m33ea	7	6	high	high	JJ
cord-021063-4y8m33ea	7	7	-	-	HYPH
cord-021063-4y8m33ea	7	8	level	level	NN
cord-021063-4y8m33ea	7	9	expression	expression	NN
cord-021063-4y8m33ea	7	10	of	of	IN
cord-021063-4y8m33ea	7	11	their	-PRON-	PRP$
cord-021063-4y8m33ea	7	12	proteins	protein	NNS
cord-021063-4y8m33ea	7	13	for	for	IN
cord-021063-4y8m33ea	7	14	only	only	RB
cord-021063-4y8m33ea	7	15	a	a	DT
cord-021063-4y8m33ea	7	16	short	short	JJ
cord-021063-4y8m33ea	7	17	time	time	NN
cord-021063-4y8m33ea	7	18	and	and	CC
cord-021063-4y8m33ea	7	19	need	nee	MD
cord-021063-4y8m33ea	7	20	not	not	RB
cord-021063-4y8m33ea	7	21	be	be	VB
cord-021063-4y8m33ea	7	22	concerned	concern	VBN
cord-021063-4y8m33ea	7	23	with	with	IN
cord-021063-4y8m33ea	7	24	the	the	DT
cord-021063-4y8m33ea	7	25	viability	viability	NN
cord-021063-4y8m33ea	7	26	of	of	IN
cord-021063-4y8m33ea	7	27	the	the	DT
cord-021063-4y8m33ea	7	28	host	host	NN
cord-021063-4y8m33ea	7	29	cell	cell	NN
cord-021063-4y8m33ea	7	30	after	after	IN
cord-021063-4y8m33ea	7	31	infection	infection	NN
cord-021063-4y8m33ea	7	32	.	.	.
cord-021063-4y8m33ea	8	1	Attempts	attempt	NNS
cord-021063-4y8m33ea	8	2	to	to	TO
cord-021063-4y8m33ea	8	3	modify	modify	VB
cord-021063-4y8m33ea	8	4	a	a	DT
cord-021063-4y8m33ea	8	5	virus	virus	NN
cord-021063-4y8m33ea	8	6	into	into	IN
cord-021063-4y8m33ea	8	7	a	a	DT
cord-021063-4y8m33ea	8	8	gene	gene	NN
cord-021063-4y8m33ea	8	9	therapy	therapy	NN
cord-021063-4y8m33ea	8	10	vector	vector	NN
cord-021063-4y8m33ea	8	11	can	can	MD
cord-021063-4y8m33ea	8	12	be	be	VB
cord-021063-4y8m33ea	8	13	hampered	hamper	VBN
cord-021063-4y8m33ea	8	14	by	by	IN
cord-021063-4y8m33ea	8	15	this	this	DT
cord-021063-4y8m33ea	8	16	conflict	conflict	NN
cord-021063-4y8m33ea	8	17	.	.	.
cord-021063-4y8m33ea	9	1	Virus	virus	NN
cord-021063-4y8m33ea	9	2	-	-	HYPH
cord-021063-4y8m33ea	9	3	based	base	VBN
cord-021063-4y8m33ea	9	4	methods	method	NNS
cord-021063-4y8m33ea	9	5	of	of	IN
cord-021063-4y8m33ea	9	6	gene	gene	NN
cord-021063-4y8m33ea	9	7	therapy	therapy	NN
cord-021063-4y8m33ea	9	8	are	be	VBP
cord-021063-4y8m33ea	9	9	likely	likely	JJ
cord-021063-4y8m33ea	9	10	to	to	TO
cord-021063-4y8m33ea	9	11	be	be	VB
cord-021063-4y8m33ea	9	12	most	most	RBS
cord-021063-4y8m33ea	9	13	useful	useful	JJ
cord-021063-4y8m33ea	9	14	in	in	IN
cord-021063-4y8m33ea	9	15	applications	application	NNS
cord-021063-4y8m33ea	9	16	that	that	WDT
cord-021063-4y8m33ea	9	17	require	require	VBP
cord-021063-4y8m33ea	9	18	a	a	DT
cord-021063-4y8m33ea	9	19	burst	burst	NN
cord-021063-4y8m33ea	9	20	of	of	IN
cord-021063-4y8m33ea	9	21	high	high	JJ
cord-021063-4y8m33ea	9	22	-	-	HYPH
cord-021063-4y8m33ea	9	23	level	level	NN
cord-021063-4y8m33ea	9	24	expression	expression	NN
cord-021063-4y8m33ea	9	25	in	in	IN
cord-021063-4y8m33ea	9	26	many	many	JJ
cord-021063-4y8m33ea	9	27	of	of	IN
cord-021063-4y8m33ea	9	28	the	the	DT
cord-021063-4y8m33ea	9	29	patient	patient	NN
cord-021063-4y8m33ea	9	30	's	's	POS
cord-021063-4y8m33ea	9	31	cells	cell	NNS
cord-021063-4y8m33ea	9	32	,	,	,
cord-021063-4y8m33ea	9	33	such	such	JJ
cord-021063-4y8m33ea	9	34	as	as	IN
cord-021063-4y8m33ea	9	35	in	in	IN
cord-021063-4y8m33ea	9	36	cancer	cancer	NN
cord-021063-4y8m33ea	9	37	therapy	therapy	NN
cord-021063-4y8m33ea	9	38	.	.	.
cord-021063-4y8m33ea	10	1	Liposomal	Liposomal	NNP
cord-021063-4y8m33ea	10	2	methods	method	NNS
cord-021063-4y8m33ea	10	3	of	of	IN
cord-021063-4y8m33ea	10	4	gene	gene	NN
cord-021063-4y8m33ea	10	5	therapy	therapy	NN
cord-021063-4y8m33ea	10	6	are	be	VBP
cord-021063-4y8m33ea	10	7	flexible	flexible	JJ
cord-021063-4y8m33ea	10	8	in	in	IN
cord-021063-4y8m33ea	10	9	that	that	IN
cord-021063-4y8m33ea	10	10	all	all	PDT
cord-021063-4y8m33ea	10	11	the	the	DT
cord-021063-4y8m33ea	10	12	components	component	NNS
cord-021063-4y8m33ea	10	13	of	of	IN
cord-021063-4y8m33ea	10	14	the	the	DT
cord-021063-4y8m33ea	10	15	system	system	NN
cord-021063-4y8m33ea	10	16	are	be	VBP
cord-021063-4y8m33ea	10	17	controlled	control	VBN
cord-021063-4y8m33ea	10	18	by	by	IN
cord-021063-4y8m33ea	10	19	designers	designer	NNS
cord-021063-4y8m33ea	10	20	.	.	.
cord-021063-4y8m33ea	11	1	As	as	IN
cord-021063-4y8m33ea	11	2	these	these	DT
cord-021063-4y8m33ea	11	3	systems	system	NNS
cord-021063-4y8m33ea	11	4	have	have	VBP
cord-021063-4y8m33ea	11	5	become	become	VBN
cord-021063-4y8m33ea	11	6	more	more	RBR
cord-021063-4y8m33ea	11	7	sophisticated	sophisticated	JJ
cord-021063-4y8m33ea	11	8	,	,	,
cord-021063-4y8m33ea	11	9	they	-PRON-	PRP
cord-021063-4y8m33ea	11	10	have	have	VBP
cord-021063-4y8m33ea	11	11	begun	begin	VBN
cord-021063-4y8m33ea	11	12	to	to	TO
cord-021063-4y8m33ea	11	13	take	take	VB
cord-021063-4y8m33ea	11	14	on	on	RP
cord-021063-4y8m33ea	11	15	several	several	JJ
cord-021063-4y8m33ea	11	16	characteristics	characteristic	NNS
cord-021063-4y8m33ea	11	17	of	of	IN
cord-021063-4y8m33ea	11	18	the	the	DT
cord-021063-4y8m33ea	11	19	viruses	virus	NNS
cord-021063-4y8m33ea	11	20	that	that	WDT
cord-021063-4y8m33ea	11	21	they	-PRON-	PRP
cord-021063-4y8m33ea	11	22	are	be	VBP
cord-021063-4y8m33ea	11	23	intended	intend	VBN
cord-021063-4y8m33ea	11	24	to	to	TO
cord-021063-4y8m33ea	11	25	replace	replace	VB
cord-021063-4y8m33ea	11	26	.	.	.
cord-021063-4y8m33ea	12	1	The	the	DT
cord-021063-4y8m33ea	12	2	use	use	NN
cord-021063-4y8m33ea	12	3	of	of	IN
cord-021063-4y8m33ea	12	4	basic	basic	JJ
cord-021063-4y8m33ea	12	5	substances	substance	NNS
cord-021063-4y8m33ea	12	6	to	to	TO
cord-021063-4y8m33ea	12	7	condense	condense	VB
cord-021063-4y8m33ea	12	8	DNA	DNA	NNP
cord-021063-4y8m33ea	12	9	has	have	VBZ
cord-021063-4y8m33ea	12	10	increased	increase	VBN
cord-021063-4y8m33ea	12	11	the	the	DT
cord-021063-4y8m33ea	12	12	efficiency	efficiency	NN
cord-021063-4y8m33ea	12	13	of	of	IN
cord-021063-4y8m33ea	12	14	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	12	15	.	.	.
cord-021063-4y8m33ea	13	1	The	the	DT
cord-021063-4y8m33ea	13	2	addition	addition	NN
cord-021063-4y8m33ea	13	3	of	of	IN
cord-021063-4y8m33ea	13	4	nucleophilic	nucleophilic	JJ
cord-021063-4y8m33ea	13	5	proteins	protein	NNS
cord-021063-4y8m33ea	13	6	raises	raise	VBZ
cord-021063-4y8m33ea	13	7	the	the	DT
cord-021063-4y8m33ea	13	8	efficiency	efficiency	NN
cord-021063-4y8m33ea	13	9	of	of	IN
cord-021063-4y8m33ea	13	10	transfection	transfection	NN
cord-021063-4y8m33ea	13	11	.	.	.
cord-021063-4y8m33ea	14	1	By	by	IN
cord-021063-4y8m33ea	14	2	adding	add	VBG
cord-021063-4y8m33ea	14	3	antibodies	antibody	NNS
cord-021063-4y8m33ea	14	4	or	or	CC
cord-021063-4y8m33ea	14	5	other	other	JJ
cord-021063-4y8m33ea	14	6	targeting	target	VBG
cord-021063-4y8m33ea	14	7	molecules	molecule	NNS
cord-021063-4y8m33ea	14	8	to	to	IN
cord-021063-4y8m33ea	14	9	the	the	DT
cord-021063-4y8m33ea	14	10	surface	surface	NN
cord-021063-4y8m33ea	14	11	of	of	IN
cord-021063-4y8m33ea	14	12	liposomes	liposome	NNS
cord-021063-4y8m33ea	14	13	,	,	,
cord-021063-4y8m33ea	14	14	preferential	preferential	JJ
cord-021063-4y8m33ea	14	15	binding	binding	NN
cord-021063-4y8m33ea	14	16	of	of	IN
cord-021063-4y8m33ea	14	17	vesicles	vesicle	NNS
cord-021063-4y8m33ea	14	18	to	to	IN
cord-021063-4y8m33ea	14	19	a	a	DT
cord-021063-4y8m33ea	14	20	desired	desire	VBN
cord-021063-4y8m33ea	14	21	cell	cell	NN
cord-021063-4y8m33ea	14	22	type	type	NN
cord-021063-4y8m33ea	14	23	has	have	VBZ
cord-021063-4y8m33ea	14	24	been	be	VBN
cord-021063-4y8m33ea	14	25	increased	increase	VBN
cord-021063-4y8m33ea	14	26	.	.	.
cord-021063-4y8m33ea	15	1	Gene	gene	NN
cord-021063-4y8m33ea	15	2	therapy	therapy	NN
cord-021063-4y8m33ea	15	3	as	as	IN
cord-021063-4y8m33ea	15	4	a	a	DT
cord-021063-4y8m33ea	15	5	treatment	treatment	NN
cord-021063-4y8m33ea	15	6	for	for	IN
cord-021063-4y8m33ea	15	7	human	human	JJ
cord-021063-4y8m33ea	15	8	disease	disease	NN
cord-021063-4y8m33ea	15	9	is	be	VBZ
cord-021063-4y8m33ea	15	10	in	in	IN
cord-021063-4y8m33ea	15	11	a	a	DT
cord-021063-4y8m33ea	15	12	stage	stage	NN
cord-021063-4y8m33ea	15	13	of	of	IN
cord-021063-4y8m33ea	15	14	intensive	intensive	JJ
cord-021063-4y8m33ea	15	15	development	development	NN
cord-021063-4y8m33ea	15	16	.	.	.
cord-021063-4y8m33ea	16	1	Already	already	RB
cord-021063-4y8m33ea	16	2	in	in	IN
cord-021063-4y8m33ea	16	3	limited	limited	JJ
cord-021063-4y8m33ea	16	4	use	use	NN
cord-021063-4y8m33ea	16	5	as	as	IN
cord-021063-4y8m33ea	16	6	an	an	DT
cord-021063-4y8m33ea	16	7	experimental	experimental	JJ
cord-021063-4y8m33ea	16	8	therapy	therapy	NN
cord-021063-4y8m33ea	16	9	for	for	IN
cord-021063-4y8m33ea	16	10	cancer	cancer	NN
cord-021063-4y8m33ea	17	1	(	(	-LRB-
cord-021063-4y8m33ea	17	2	Oldfield	Oldfield	NNP
cord-021063-4y8m33ea	17	3	et	et	NNP
cord-021063-4y8m33ea	17	4	al	al	NNP
cord-021063-4y8m33ea	17	5	.	.	NNP
cord-021063-4y8m33ea	17	6	,	,	,
cord-021063-4y8m33ea	17	7	1993	1993	CD
cord-021063-4y8m33ea	17	8	;	;	:
cord-021063-4y8m33ea	18	1	Nabel	Nabel	NNP
cord-021063-4y8m33ea	18	2	et	et	NNP
cord-021063-4y8m33ea	18	3	al	al	NNP
cord-021063-4y8m33ea	18	4	.	.	NNP
cord-021063-4y8m33ea	18	5	,	,	,
cord-021063-4y8m33ea	18	6	1993	1993	CD
cord-021063-4y8m33ea	18	7	)	)	-RRB-
cord-021063-4y8m33ea	18	8	and	and	CC
cord-021063-4y8m33ea	18	9	inborn	inborn	JJ
cord-021063-4y8m33ea	18	10	errors	error	NNS
cord-021063-4y8m33ea	18	11	of	of	IN
cord-021063-4y8m33ea	18	12	metabolism	metabolism	NN
cord-021063-4y8m33ea	19	1	(	(	-LRB-
cord-021063-4y8m33ea	19	2	Hoogerbrugge	Hoogerbrugge	NNP
cord-021063-4y8m33ea	19	3	et	et	NNP
cord-021063-4y8m33ea	19	4	al	al	NNP
cord-021063-4y8m33ea	19	5	.	.	.
cord-021063-4y8m33ea	20	1	,	,	,
cord-021063-4y8m33ea	20	2	1992	1992	CD
cord-021063-4y8m33ea	20	3	;	;	:
cord-021063-4y8m33ea	20	4	Morgan	Morgan	NNP
cord-021063-4y8m33ea	20	5	and	and	CC
cord-021063-4y8m33ea	20	6	Anderson	Anderson	NNP
cord-021063-4y8m33ea	20	7	,	,	,
cord-021063-4y8m33ea	20	8	1993	1993	CD
cord-021063-4y8m33ea	20	9	)	)	-RRB-
cord-021063-4y8m33ea	20	10	,	,	,
cord-021063-4y8m33ea	20	11	it	-PRON-	PRP
cord-021063-4y8m33ea	20	12	will	will	MD
cord-021063-4y8m33ea	20	13	become	become	VB
cord-021063-4y8m33ea	20	14	a	a	DT
cord-021063-4y8m33ea	20	15	tool	tool	NN
cord-021063-4y8m33ea	20	16	to	to	TO
cord-021063-4y8m33ea	20	17	treat	treat	VB
cord-021063-4y8m33ea	20	18	diseases	disease	NNS
cord-021063-4y8m33ea	20	19	that	that	WDT
cord-021063-4y8m33ea	20	20	are	be	VBP
cord-021063-4y8m33ea	20	21	currently	currently	RB
cord-021063-4y8m33ea	20	22	difficult	difficult	JJ
cord-021063-4y8m33ea	20	23	to	to	TO
cord-021063-4y8m33ea	20	24	manage	manage	VB
cord-021063-4y8m33ea	20	25	.	.	.
cord-021063-4y8m33ea	21	1	There	there	EX
cord-021063-4y8m33ea	21	2	are	be	VBP
cord-021063-4y8m33ea	21	3	many	many	JJ
cord-021063-4y8m33ea	21	4	competing	compete	VBG
cord-021063-4y8m33ea	21	5	strategies	strategy	NNS
cord-021063-4y8m33ea	21	6	being	be	VBG
cord-021063-4y8m33ea	21	7	developed	develop	VBN
cord-021063-4y8m33ea	21	8	to	to	TO
cord-021063-4y8m33ea	21	9	introduce	introduce	VB
cord-021063-4y8m33ea	21	10	exogenous	exogenous	JJ
cord-021063-4y8m33ea	21	11	genes	gene	NNS
cord-021063-4y8m33ea	21	12	into	into	IN
cord-021063-4y8m33ea	21	13	a	a	DT
cord-021063-4y8m33ea	21	14	subset	subset	NN
cord-021063-4y8m33ea	21	15	of	of	IN
cord-021063-4y8m33ea	21	16	cells	cell	NNS
cord-021063-4y8m33ea	21	17	of	of	IN
cord-021063-4y8m33ea	21	18	a	a	DT
cord-021063-4y8m33ea	21	19	patient	patient	NN
cord-021063-4y8m33ea	21	20	.	.	.
cord-021063-4y8m33ea	22	1	Several	several	JJ
cord-021063-4y8m33ea	22	2	of	of	IN
cord-021063-4y8m33ea	22	3	these	these	DT
cord-021063-4y8m33ea	22	4	are	be	VBP
cord-021063-4y8m33ea	22	5	likely	likely	JJ
cord-021063-4y8m33ea	22	6	to	to	TO
cord-021063-4y8m33ea	22	7	proceed	proceed	VB
cord-021063-4y8m33ea	22	8	to	to	IN
cord-021063-4y8m33ea	22	9	clinical	clinical	JJ
cord-021063-4y8m33ea	22	10	application	application	NN
cord-021063-4y8m33ea	22	11	.	.	.
cord-021063-4y8m33ea	23	1	Deciding	decide	VBG
cord-021063-4y8m33ea	23	2	which	which	WDT
cord-021063-4y8m33ea	23	3	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	23	4	strategy	strategy	NN
cord-021063-4y8m33ea	23	5	is	be	VBZ
cord-021063-4y8m33ea	23	6	best	well	RBS
cord-021063-4y8m33ea	23	7	suited	suit	VBN
cord-021063-4y8m33ea	23	8	for	for	IN
cord-021063-4y8m33ea	23	9	a	a	DT
cord-021063-4y8m33ea	23	10	given	give	VBN
cord-021063-4y8m33ea	23	11	disease	disease	NN
cord-021063-4y8m33ea	23	12	will	will	MD
cord-021063-4y8m33ea	23	13	require	require	VB
cord-021063-4y8m33ea	23	14	an	an	DT
cord-021063-4y8m33ea	23	15	understanding	understanding	NN
cord-021063-4y8m33ea	23	16	of	of	IN
cord-021063-4y8m33ea	23	17	the	the	DT
cord-021063-4y8m33ea	23	18	underlying	underlying	JJ
cord-021063-4y8m33ea	23	19	biology	biology	NN
cord-021063-4y8m33ea	23	20	of	of	IN
cord-021063-4y8m33ea	23	21	the	the	DT
cord-021063-4y8m33ea	23	22	disease	disease	NN
cord-021063-4y8m33ea	23	23	,	,	,
cord-021063-4y8m33ea	23	24	as	as	RB
cord-021063-4y8m33ea	23	25	well	well	RB
cord-021063-4y8m33ea	23	26	as	as	IN
cord-021063-4y8m33ea	23	27	the	the	DT
cord-021063-4y8m33ea	23	28	therapy	therapy	NN
cord-021063-4y8m33ea	23	29	.	.	.
cord-021063-4y8m33ea	24	1	In	in	IN
cord-021063-4y8m33ea	24	2	using	use	VBG
cord-021063-4y8m33ea	24	3	this	this	DT
cord-021063-4y8m33ea	24	4	chapter	chapter	NN
cord-021063-4y8m33ea	24	5	to	to	TO
cord-021063-4y8m33ea	24	6	learn	learn	VB
cord-021063-4y8m33ea	24	7	about	about	IN
cord-021063-4y8m33ea	24	8	liposomal	liposomal	JJ
cord-021063-4y8m33ea	24	9	methods	method	NNS
cord-021063-4y8m33ea	24	10	of	of	IN
cord-021063-4y8m33ea	24	11	gene	gene	NN
cord-021063-4y8m33ea	24	12	therapy	therapy	NN
cord-021063-4y8m33ea	24	13	,	,	,
cord-021063-4y8m33ea	24	14	one	one	PRP
cord-021063-4y8m33ea	24	15	should	should	MD
cord-021063-4y8m33ea	24	16	remember	remember	VB
cord-021063-4y8m33ea	24	17	that	that	IN
cord-021063-4y8m33ea	24	18	the	the	DT
cord-021063-4y8m33ea	24	19	material	material	NN
cord-021063-4y8m33ea	24	20	presented	present	VBN
cord-021063-4y8m33ea	24	21	here	here	RB
cord-021063-4y8m33ea	24	22	is	be	VBZ
cord-021063-4y8m33ea	24	23	only	only	RB
cord-021063-4y8m33ea	24	24	current	current	JJ
cord-021063-4y8m33ea	24	25	as	as	IN
cord-021063-4y8m33ea	24	26	of	of	IN
cord-021063-4y8m33ea	24	27	January	January	NNP
cord-021063-4y8m33ea	24	28	,	,	,
cord-021063-4y8m33ea	24	29	1994	1994	CD
cord-021063-4y8m33ea	24	30	.	.	.
cord-021063-4y8m33ea	25	1	For	for	IN
cord-021063-4y8m33ea	25	2	more	more	JJR
cord-021063-4y8m33ea	25	3	recent	recent	JJ
cord-021063-4y8m33ea	25	4	developments	development	NNS
cord-021063-4y8m33ea	25	5	and	and	CC
cord-021063-4y8m33ea	25	6	greater	great	JJR
cord-021063-4y8m33ea	25	7	detail	detail	NN
cord-021063-4y8m33ea	25	8	,	,	,
cord-021063-4y8m33ea	25	9	it	-PRON-	PRP
cord-021063-4y8m33ea	25	10	will	will	MD
cord-021063-4y8m33ea	25	11	be	be	VB
cord-021063-4y8m33ea	25	12	necessary	necessary	JJ
cord-021063-4y8m33ea	25	13	to	to	TO
cord-021063-4y8m33ea	25	14	consult	consult	VB
cord-021063-4y8m33ea	25	15	the	the	DT
cord-021063-4y8m33ea	25	16	original	original	JJ
cord-021063-4y8m33ea	25	17	literature	literature	NN
cord-021063-4y8m33ea	25	18	.	.	.
cord-021063-4y8m33ea	26	1	Regardless	regardless	RB
cord-021063-4y8m33ea	26	2	of	of	IN
cord-021063-4y8m33ea	26	3	the	the	DT
cord-021063-4y8m33ea	26	4	specific	specific	JJ
cord-021063-4y8m33ea	26	5	disease	disease	NN
cord-021063-4y8m33ea	26	6	being	be	VBG
cord-021063-4y8m33ea	26	7	addressed	address	VBN
cord-021063-4y8m33ea	26	8	by	by	IN
cord-021063-4y8m33ea	26	9	gene	gene	NN
cord-021063-4y8m33ea	26	10	therapy	therapy	NN
cord-021063-4y8m33ea	26	11	,	,	,
cord-021063-4y8m33ea	26	12	several	several	JJ
cord-021063-4y8m33ea	26	13	factors	factor	NNS
cord-021063-4y8m33ea	26	14	must	must	MD
cord-021063-4y8m33ea	26	15	be	be	VB
cord-021063-4y8m33ea	26	16	considered	consider	VBN
cord-021063-4y8m33ea	26	17	before	before	IN
cord-021063-4y8m33ea	26	18	one	one	CD
cord-021063-4y8m33ea	26	19	treats	treat	VBZ
cord-021063-4y8m33ea	26	20	patients	patient	NNS
cord-021063-4y8m33ea	26	21	.	.	.
cord-021063-4y8m33ea	27	1	The	the	DT
cord-021063-4y8m33ea	27	2	first	first	JJ
cord-021063-4y8m33ea	27	3	is	be	VBZ
cord-021063-4y8m33ea	27	4	that	that	IN
cord-021063-4y8m33ea	27	5	the	the	DT
cord-021063-4y8m33ea	27	6	method	method	NN
cord-021063-4y8m33ea	27	7	and	and	CC
cord-021063-4y8m33ea	27	8	the	the	DT
cord-021063-4y8m33ea	27	9	treatment	treatment	NN
cord-021063-4y8m33ea	27	10	itself	-PRON-	PRP
cord-021063-4y8m33ea	27	11	must	must	MD
cord-021063-4y8m33ea	27	12	be	be	VB
cord-021063-4y8m33ea	27	13	commensurate	commensurate	JJ
cord-021063-4y8m33ea	27	14	with	with	IN
cord-021063-4y8m33ea	27	15	the	the	DT
cord-021063-4y8m33ea	27	16	severity	severity	NN
cord-021063-4y8m33ea	27	17	of	of	IN
cord-021063-4y8m33ea	27	18	the	the	DT
cord-021063-4y8m33ea	27	19	disease	disease	NN
cord-021063-4y8m33ea	27	20	.	.	.
cord-021063-4y8m33ea	28	1	Gene	gene	NN
cord-021063-4y8m33ea	28	2	therapy	therapy	NN
cord-021063-4y8m33ea	28	3	will	will	MD
cord-021063-4y8m33ea	28	4	not	not	RB
cord-021063-4y8m33ea	28	5	be	be	VB
cord-021063-4y8m33ea	28	6	the	the	DT
cord-021063-4y8m33ea	28	7	preferred	preferred	JJ
cord-021063-4y8m33ea	28	8	treatment	treatment	NN
cord-021063-4y8m33ea	28	9	for	for	IN
cord-021063-4y8m33ea	28	10	diseases	disease	NNS
cord-021063-4y8m33ea	28	11	such	such	JJ
cord-021063-4y8m33ea	28	12	as	as	IN
cord-021063-4y8m33ea	28	13	phenylketonuria	phenylketonuria	NN
cord-021063-4y8m33ea	28	14	or	or	CC
cord-021063-4y8m33ea	28	15	galactosemia	galactosemia	NN
cord-021063-4y8m33ea	28	16	,	,	,
cord-021063-4y8m33ea	28	17	both	both	DT
cord-021063-4y8m33ea	28	18	of	of	IN
cord-021063-4y8m33ea	28	19	which	which	WDT
cord-021063-4y8m33ea	28	20	can	can	MD
cord-021063-4y8m33ea	28	21	be	be	VB
cord-021063-4y8m33ea	28	22	treated	treat	VBN
cord-021063-4y8m33ea	28	23	adequately	adequately	RB
cord-021063-4y8m33ea	28	24	by	by	IN
cord-021063-4y8m33ea	28	25	diet	diet	NN
cord-021063-4y8m33ea	28	26	modification	modification	NN
cord-021063-4y8m33ea	28	27	.	.	.
cord-021063-4y8m33ea	29	1	A	a	DT
cord-021063-4y8m33ea	29	2	more	more	RBR
cord-021063-4y8m33ea	29	3	widely	widely	RB
cord-021063-4y8m33ea	29	4	recognized	recognize	VBN
cord-021063-4y8m33ea	29	5	problem	problem	NN
cord-021063-4y8m33ea	29	6	is	be	VBZ
cord-021063-4y8m33ea	29	7	that	that	IN
cord-021063-4y8m33ea	29	8	the	the	DT
cord-021063-4y8m33ea	29	9	treatment	treatment	NN
cord-021063-4y8m33ea	29	10	may	may	MD
cord-021063-4y8m33ea	29	11	,	,	,
cord-021063-4y8m33ea	29	12	while	while	IN
cord-021063-4y8m33ea	29	13	curing	cure	VBG
cord-021063-4y8m33ea	29	14	the	the	DT
cord-021063-4y8m33ea	29	15	disease	disease	NN
cord-021063-4y8m33ea	29	16	,	,	,
cord-021063-4y8m33ea	29	17	create	create	VB
cord-021063-4y8m33ea	29	18	a	a	DT
cord-021063-4y8m33ea	29	19	new	new	JJ
cord-021063-4y8m33ea	29	20	one	one	CD
cord-021063-4y8m33ea	29	21	in	in	IN
cord-021063-4y8m33ea	29	22	the	the	DT
cord-021063-4y8m33ea	29	23	process	process	NN
cord-021063-4y8m33ea	29	24	.	.	.
cord-021063-4y8m33ea	30	1	The	the	DT
cord-021063-4y8m33ea	30	2	archetypal	archetypal	JJ
cord-021063-4y8m33ea	30	3	concern	concern	NN
cord-021063-4y8m33ea	30	4	is	be	VBZ
cord-021063-4y8m33ea	30	5	the	the	DT
cord-021063-4y8m33ea	30	6	generation	generation	NN
cord-021063-4y8m33ea	30	7	of	of	IN
cord-021063-4y8m33ea	30	8	insertional	insertional	JJ
cord-021063-4y8m33ea	30	9	mutations	mutation	NNS
cord-021063-4y8m33ea	30	10	by	by	IN
cord-021063-4y8m33ea	30	11	the	the	DT
cord-021063-4y8m33ea	30	12	integration	integration	NN
cord-021063-4y8m33ea	30	13	of	of	IN
cord-021063-4y8m33ea	30	14	the	the	DT
cord-021063-4y8m33ea	30	15	curative	curative	JJ
cord-021063-4y8m33ea	30	16	gene	gene	NN
cord-021063-4y8m33ea	30	17	into	into	IN
cord-021063-4y8m33ea	30	18	a	a	DT
cord-021063-4y8m33ea	30	19	cellular	cellular	JJ
cord-021063-4y8m33ea	30	20	protooncogene	protooncogene	NN
cord-021063-4y8m33ea	30	21	,	,	,
cord-021063-4y8m33ea	30	22	leading	lead	VBG
cord-021063-4y8m33ea	30	23	to	to	IN
cord-021063-4y8m33ea	30	24	cancer	cancer	NN
cord-021063-4y8m33ea	30	25	in	in	IN
cord-021063-4y8m33ea	30	26	the	the	DT
cord-021063-4y8m33ea	30	27	"	"	``
cord-021063-4y8m33ea	30	28	cured	cure	VBN
cord-021063-4y8m33ea	30	29	"	"	''
cord-021063-4y8m33ea	30	30	patient	patient	JJ
cord-021063-4y8m33ea	30	31	.	.	.
cord-021063-4y8m33ea	31	1	Once	once	RB
cord-021063-4y8m33ea	31	2	a	a	DT
cord-021063-4y8m33ea	31	3	method	method	NN
cord-021063-4y8m33ea	31	4	has	have	VBZ
cord-021063-4y8m33ea	31	5	been	be	VBN
cord-021063-4y8m33ea	31	6	determined	determine	VBN
cord-021063-4y8m33ea	31	7	to	to	TO
cord-021063-4y8m33ea	31	8	be	be	VB
cord-021063-4y8m33ea	31	9	reasonably	reasonably	RB
cord-021063-4y8m33ea	31	10	safe	safe	JJ
cord-021063-4y8m33ea	31	11	for	for	IN
cord-021063-4y8m33ea	31	12	the	the	DT
cord-021063-4y8m33ea	31	13	patient	patient	NN
cord-021063-4y8m33ea	31	14	,	,	,
cord-021063-4y8m33ea	31	15	it	-PRON-	PRP
cord-021063-4y8m33ea	31	16	must	must	MD
cord-021063-4y8m33ea	31	17	be	be	VB
cord-021063-4y8m33ea	31	18	shown	show	VBN
cord-021063-4y8m33ea	31	19	not	not	RB
cord-021063-4y8m33ea	31	20	to	to	TO
cord-021063-4y8m33ea	31	21	present	present	VB
cord-021063-4y8m33ea	31	22	a	a	DT
cord-021063-4y8m33ea	31	23	danger	danger	NN
cord-021063-4y8m33ea	31	24	to	to	IN
cord-021063-4y8m33ea	31	25	others	other	NNS
cord-021063-4y8m33ea	31	26	.	.	.
cord-021063-4y8m33ea	32	1	This	this	DT
cord-021063-4y8m33ea	32	2	problem	problem	NN
cord-021063-4y8m33ea	32	3	has	have	VBZ
cord-021063-4y8m33ea	32	4	hampered	hamper	VBN
cord-021063-4y8m33ea	32	5	the	the	DT
cord-021063-4y8m33ea	32	6	widespread	widespread	JJ
cord-021063-4y8m33ea	32	7	use	use	NN
cord-021063-4y8m33ea	32	8	of	of	IN
cord-021063-4y8m33ea	32	9	viral	viral	JJ
cord-021063-4y8m33ea	32	10	transfection	transfection	NN
cord-021063-4y8m33ea	32	11	vectors	vector	NNS
cord-021063-4y8m33ea	32	12	.	.	.
cord-021063-4y8m33ea	33	1	Substantial	substantial	JJ
cord-021063-4y8m33ea	33	2	progress	progress	NN
cord-021063-4y8m33ea	33	3	has	have	VBZ
cord-021063-4y8m33ea	33	4	been	be	VBN
cord-021063-4y8m33ea	33	5	made	make	VBN
cord-021063-4y8m33ea	33	6	in	in	IN
cord-021063-4y8m33ea	33	7	the	the	DT
cord-021063-4y8m33ea	33	8	construction	construction	NN
cord-021063-4y8m33ea	33	9	of	of	IN
cord-021063-4y8m33ea	33	10	replication	replication	NN
cord-021063-4y8m33ea	33	11	-	-	HYPH
cord-021063-4y8m33ea	33	12	defective	defective	JJ
cord-021063-4y8m33ea	33	13	retroviral	retroviral	NN
cord-021063-4y8m33ea	33	14	and	and	CC
cord-021063-4y8m33ea	33	15	adenoviral	adenoviral	JJ
cord-021063-4y8m33ea	33	16	vectors	vector	NNS
cord-021063-4y8m33ea	33	17	.	.	.
cord-021063-4y8m33ea	34	1	However	however	RB
cord-021063-4y8m33ea	34	2	,	,	,
cord-021063-4y8m33ea	34	3	the	the	DT
cord-021063-4y8m33ea	34	4	possibility	possibility	NN
cord-021063-4y8m33ea	34	5	remains	remain	VBZ
cord-021063-4y8m33ea	34	6	that	that	IN
cord-021063-4y8m33ea	34	7	superinfection	superinfection	NN
cord-021063-4y8m33ea	34	8	with	with	IN
cord-021063-4y8m33ea	34	9	wild	wild	JJ
cord-021063-4y8m33ea	34	10	-	-	HYPH
cord-021063-4y8m33ea	34	11	type	type	NN
cord-021063-4y8m33ea	34	12	adenovirus	adenovirus	NN
cord-021063-4y8m33ea	34	13	,	,	,
cord-021063-4y8m33ea	34	14	or	or	CC
cord-021063-4y8m33ea	34	15	insertion	insertion	NN
cord-021063-4y8m33ea	34	16	of	of	IN
cord-021063-4y8m33ea	34	17	a	a	DT
cord-021063-4y8m33ea	34	18	wild	wild	JJ
cord-021063-4y8m33ea	34	19	-	-	HYPH
cord-021063-4y8m33ea	34	20	type	type	NN
cord-021063-4y8m33ea	34	21	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	34	22	into	into	IN
cord-021063-4y8m33ea	34	23	an	an	DT
cord-021063-4y8m33ea	34	24	integrated	integrate	VBN
cord-021063-4y8m33ea	34	25	defective	defective	JJ
cord-021063-4y8m33ea	34	26	retroviral	retroviral	NN
cord-021063-4y8m33ea	34	27	genome	genome	NN
cord-021063-4y8m33ea	34	28	,	,	,
cord-021063-4y8m33ea	34	29	can	can	MD
cord-021063-4y8m33ea	34	30	generate	generate	VB
cord-021063-4y8m33ea	34	31	new	new	JJ
cord-021063-4y8m33ea	34	32	,	,	,
cord-021063-4y8m33ea	34	33	infectious	infectious	JJ
cord-021063-4y8m33ea	34	34	viral	viral	JJ
cord-021063-4y8m33ea	34	35	particles	particle	NNS
cord-021063-4y8m33ea	34	36	that	that	WDT
cord-021063-4y8m33ea	34	37	could	could	MD
cord-021063-4y8m33ea	34	38	contain	contain	VB
cord-021063-4y8m33ea	34	39	an	an	DT
cord-021063-4y8m33ea	34	40	oncogenic	oncogenic	JJ
cord-021063-4y8m33ea	34	41	fragment	fragment	NN
cord-021063-4y8m33ea	34	42	of	of	IN
cord-021063-4y8m33ea	34	43	the	the	DT
cord-021063-4y8m33ea	34	44	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	34	45	gene	gene	NN
cord-021063-4y8m33ea	34	46	.	.	.
cord-021063-4y8m33ea	35	1	These	these	DT
cord-021063-4y8m33ea	35	2	viral	viral	JJ
cord-021063-4y8m33ea	35	3	particles	particle	NNS
cord-021063-4y8m33ea	35	4	may	may	MD
cord-021063-4y8m33ea	35	5	have	have	VB
cord-021063-4y8m33ea	35	6	the	the	DT
cord-021063-4y8m33ea	35	7	ability	ability	NN
cord-021063-4y8m33ea	35	8	to	to	TO
cord-021063-4y8m33ea	35	9	transfer	transfer	VB
cord-021063-4y8m33ea	35	10	genes	gene	NNS
cord-021063-4y8m33ea	35	11	between	between	IN
cord-021063-4y8m33ea	35	12	a	a	DT
cord-021063-4y8m33ea	35	13	patient	patient	NN
cord-021063-4y8m33ea	35	14	's	's	POS
cord-021063-4y8m33ea	35	15	cells	cell	NNS
cord-021063-4y8m33ea	35	16	,	,	,
cord-021063-4y8m33ea	35	17	and	and	CC
cord-021063-4y8m33ea	35	18	between	between	IN
cord-021063-4y8m33ea	35	19	individuals	individual	NNS
cord-021063-4y8m33ea	35	20	.	.	.
cord-021063-4y8m33ea	36	1	In	in	IN
cord-021063-4y8m33ea	36	2	order	order	NN
cord-021063-4y8m33ea	36	3	for	for	IN
cord-021063-4y8m33ea	36	4	a	a	DT
cord-021063-4y8m33ea	36	5	method	method	NN
cord-021063-4y8m33ea	36	6	of	of	IN
cord-021063-4y8m33ea	36	7	gene	gene	NN
cord-021063-4y8m33ea	36	8	therapy	therapy	NN
cord-021063-4y8m33ea	36	9	to	to	TO
cord-021063-4y8m33ea	36	10	be	be	VB
cord-021063-4y8m33ea	36	11	useful	useful	JJ
cord-021063-4y8m33ea	36	12	,	,	,
cord-021063-4y8m33ea	36	13	it	-PRON-	PRP
cord-021063-4y8m33ea	36	14	must	must	MD
cord-021063-4y8m33ea	36	15	significantly	significantly	RB
cord-021063-4y8m33ea	36	16	ameliorate	ameliorate	VB
cord-021063-4y8m33ea	36	17	the	the	DT
cord-021063-4y8m33ea	36	18	symptoms	symptom	NNS
cord-021063-4y8m33ea	36	19	of	of	IN
cord-021063-4y8m33ea	36	20	the	the	DT
cord-021063-4y8m33ea	36	21	disease	disease	NN
cord-021063-4y8m33ea	36	22	,	,	,
cord-021063-4y8m33ea	36	23	and	and	CC
cord-021063-4y8m33ea	36	24	halt	halt	VB
cord-021063-4y8m33ea	36	25	the	the	DT
cord-021063-4y8m33ea	36	26	progression	progression	NN
cord-021063-4y8m33ea	36	27	of	of	IN
cord-021063-4y8m33ea	36	28	the	the	DT
cord-021063-4y8m33ea	36	29	disease	disease	NN
cord-021063-4y8m33ea	36	30	state	state	NN
cord-021063-4y8m33ea	36	31	for	for	IN
cord-021063-4y8m33ea	36	32	a	a	DT
cord-021063-4y8m33ea	36	33	significant	significant	JJ
cord-021063-4y8m33ea	36	34	period	period	NN
cord-021063-4y8m33ea	36	35	of	of	IN
cord-021063-4y8m33ea	36	36	time	time	NN
cord-021063-4y8m33ea	36	37	.	.	.
cord-021063-4y8m33ea	37	1	Ideally	ideally	RB
cord-021063-4y8m33ea	37	2	,	,	,
cord-021063-4y8m33ea	37	3	the	the	DT
cord-021063-4y8m33ea	37	4	disease	disease	NN
cord-021063-4y8m33ea	37	5	would	would	MD
cord-021063-4y8m33ea	37	6	be	be	VB
cord-021063-4y8m33ea	37	7	cured	cure	VBN
cord-021063-4y8m33ea	37	8	permanently	permanently	RB
cord-021063-4y8m33ea	37	9	by	by	IN
cord-021063-4y8m33ea	37	10	one	one	CD
cord-021063-4y8m33ea	37	11	treatment	treatment	NN
cord-021063-4y8m33ea	37	12	.	.	.
cord-021063-4y8m33ea	38	1	Although	although	IN
cord-021063-4y8m33ea	38	2	periodic	periodic	JJ
cord-021063-4y8m33ea	38	3	retreatments	retreatment	NNS
cord-021063-4y8m33ea	38	4	might	may	MD
cord-021063-4y8m33ea	38	5	be	be	VB
cord-021063-4y8m33ea	38	6	acceptable	acceptable	JJ
cord-021063-4y8m33ea	38	7	,	,	,
cord-021063-4y8m33ea	38	8	every	every	DT
cord-021063-4y8m33ea	38	9	treatment	treatment	NN
cord-021063-4y8m33ea	38	10	brings	bring	VBZ
cord-021063-4y8m33ea	38	11	with	with	IN
cord-021063-4y8m33ea	38	12	it	-PRON-	PRP
cord-021063-4y8m33ea	38	13	the	the	DT
cord-021063-4y8m33ea	38	14	possibility	possibility	NN
cord-021063-4y8m33ea	38	15	of	of	IN
cord-021063-4y8m33ea	38	16	insertional	insertional	JJ
cord-021063-4y8m33ea	38	17	mutagenesis	mutagenesis	NN
cord-021063-4y8m33ea	38	18	or	or	CC
cord-021063-4y8m33ea	38	19	the	the	DT
cord-021063-4y8m33ea	38	20	generation	generation	NN
cord-021063-4y8m33ea	38	21	of	of	IN
cord-021063-4y8m33ea	38	22	an	an	DT
cord-021063-4y8m33ea	38	23	immune	immune	JJ
cord-021063-4y8m33ea	38	24	response	response	NN
cord-021063-4y8m33ea	38	25	to	to	IN
cord-021063-4y8m33ea	38	26	the	the	DT
cord-021063-4y8m33ea	38	27	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	38	28	vector	vector	NN
cord-021063-4y8m33ea	38	29	.	.	.
cord-021063-4y8m33ea	39	1	The	the	DT
cord-021063-4y8m33ea	39	2	final	final	JJ
cord-021063-4y8m33ea	39	3	considerations	consideration	NNS
cord-021063-4y8m33ea	39	4	involve	involve	VBP
cord-021063-4y8m33ea	39	5	ease	ease	NN
cord-021063-4y8m33ea	39	6	of	of	IN
cord-021063-4y8m33ea	39	7	preparation	preparation	NN
cord-021063-4y8m33ea	39	8	and	and	CC
cord-021063-4y8m33ea	39	9	use	use	NN
cord-021063-4y8m33ea	39	10	.	.	.
cord-021063-4y8m33ea	40	1	Many	many	JJ
cord-021063-4y8m33ea	40	2	candidate	candidate	NN
cord-021063-4y8m33ea	40	3	vectors	vector	NNS
cord-021063-4y8m33ea	40	4	,	,	,
cord-021063-4y8m33ea	40	5	although	although	IN
cord-021063-4y8m33ea	40	6	potentially	potentially	RB
cord-021063-4y8m33ea	40	7	capable	capable	JJ
cord-021063-4y8m33ea	40	8	of	of	IN
cord-021063-4y8m33ea	40	9	introducing	introduce	VBG
cord-021063-4y8m33ea	40	10	foreign	foreign	JJ
cord-021063-4y8m33ea	40	11	genes	gene	NNS
cord-021063-4y8m33ea	40	12	into	into	IN
cord-021063-4y8m33ea	40	13	an	an	DT
cord-021063-4y8m33ea	40	14	organism	organism	NN
cord-021063-4y8m33ea	40	15	,	,	,
cord-021063-4y8m33ea	40	16	are	be	VBP
cord-021063-4y8m33ea	40	17	so	so	RB
cord-021063-4y8m33ea	40	18	difficult	difficult	JJ
cord-021063-4y8m33ea	40	19	to	to	TO
cord-021063-4y8m33ea	40	20	generate	generate	VB
cord-021063-4y8m33ea	40	21	,	,	,
cord-021063-4y8m33ea	40	22	or	or	CC
cord-021063-4y8m33ea	40	23	administer	administer	VB
cord-021063-4y8m33ea	40	24	,	,	,
cord-021063-4y8m33ea	40	25	that	that	IN
cord-021063-4y8m33ea	40	26	it	-PRON-	PRP
cord-021063-4y8m33ea	40	27	is	be	VBZ
cord-021063-4y8m33ea	40	28	difficult	difficult	JJ
cord-021063-4y8m33ea	40	29	to	to	TO
cord-021063-4y8m33ea	40	30	envision	envision	VB
cord-021063-4y8m33ea	40	31	their	-PRON-	PRP$
cord-021063-4y8m33ea	40	32	widespread	widespread	JJ
cord-021063-4y8m33ea	40	33	adoption	adoption	NN
cord-021063-4y8m33ea	40	34	.	.	.
cord-021063-4y8m33ea	41	1	This	this	DT
cord-021063-4y8m33ea	41	2	aspect	aspect	NN
cord-021063-4y8m33ea	41	3	of	of	IN
cord-021063-4y8m33ea	41	4	vector	vector	NN
cord-021063-4y8m33ea	41	5	design	design	NN
cord-021063-4y8m33ea	41	6	is	be	VBZ
cord-021063-4y8m33ea	41	7	often	often	RB
cord-021063-4y8m33ea	41	8	not	not	RB
cord-021063-4y8m33ea	41	9	considered	consider	VBN
cord-021063-4y8m33ea	41	10	until	until	IN
cord-021063-4y8m33ea	41	11	after	after	IN
cord-021063-4y8m33ea	41	12	the	the	DT
cord-021063-4y8m33ea	41	13	vector	vector	NN
cord-021063-4y8m33ea	41	14	is	be	VBZ
cord-021063-4y8m33ea	41	15	developed	develop	VBN
cord-021063-4y8m33ea	41	16	.	.	.
cord-021063-4y8m33ea	42	1	Bearing	bear	VBG
cord-021063-4y8m33ea	42	2	in	in	IN
cord-021063-4y8m33ea	42	3	mind	mind	NN
cord-021063-4y8m33ea	42	4	the	the	DT
cord-021063-4y8m33ea	42	5	above	above	JJ
cord-021063-4y8m33ea	42	6	considerations	consideration	NNS
cord-021063-4y8m33ea	42	7	,	,	,
cord-021063-4y8m33ea	42	8	let	let	VB
cord-021063-4y8m33ea	42	9	us	-PRON-	PRP
cord-021063-4y8m33ea	42	10	now	now	RB
cord-021063-4y8m33ea	42	11	consider	consider	VB
cord-021063-4y8m33ea	42	12	the	the	DT
cord-021063-4y8m33ea	42	13	various	various	JJ
cord-021063-4y8m33ea	42	14	approaches	approach	NNS
cord-021063-4y8m33ea	42	15	being	be	VBG
cord-021063-4y8m33ea	42	16	developed	develop	VBN
cord-021063-4y8m33ea	42	17	to	to	TO
cord-021063-4y8m33ea	42	18	create	create	VB
cord-021063-4y8m33ea	42	19	vectors	vector	NNS
cord-021063-4y8m33ea	42	20	for	for	IN
cord-021063-4y8m33ea	42	21	gene	gene	NN
cord-021063-4y8m33ea	42	22	therapy	therapy	NN
cord-021063-4y8m33ea	42	23	.	.	.
cord-021063-4y8m33ea	43	1	In	in	IN
cord-021063-4y8m33ea	43	2	general	general	JJ
cord-021063-4y8m33ea	43	3	,	,	,
cord-021063-4y8m33ea	43	4	these	these	DT
cord-021063-4y8m33ea	43	5	can	can	MD
cord-021063-4y8m33ea	43	6	be	be	VB
cord-021063-4y8m33ea	43	7	divided	divide	VBN
cord-021063-4y8m33ea	43	8	into	into	IN
cord-021063-4y8m33ea	43	9	three	three	CD
cord-021063-4y8m33ea	43	10	groups	group	NNS
cord-021063-4y8m33ea	43	11	:	:	:
cord-021063-4y8m33ea	43	12	(	(	-LRB-
cord-021063-4y8m33ea	43	13	i	i	NN
cord-021063-4y8m33ea	43	14	)	)	-RRB-
cord-021063-4y8m33ea	43	15	active	active	JJ
cord-021063-4y8m33ea	43	16	viruses	virus	NNS
cord-021063-4y8m33ea	43	17	,	,	,
cord-021063-4y8m33ea	43	18	(	(	-LRB-
cord-021063-4y8m33ea	43	19	ii	ii	NN
cord-021063-4y8m33ea	43	20	)	)	-RRB-
cord-021063-4y8m33ea	43	21	viral	viral	JJ
cord-021063-4y8m33ea	43	22	mimics	mimic	VBZ
cord-021063-4y8m33ea	43	23	that	that	WDT
cord-021063-4y8m33ea	43	24	attempt	attempt	VBP
cord-021063-4y8m33ea	43	25	to	to	TO
cord-021063-4y8m33ea	43	26	reproduce	reproduce	VB
cord-021063-4y8m33ea	43	27	a	a	DT
cord-021063-4y8m33ea	43	28	subset	subset	NN
cord-021063-4y8m33ea	43	29	of	of	IN
cord-021063-4y8m33ea	43	30	viral	viral	JJ
cord-021063-4y8m33ea	43	31	activities	activity	NNS
cord-021063-4y8m33ea	43	32	in	in	IN
cord-021063-4y8m33ea	43	33	a	a	DT
cord-021063-4y8m33ea	43	34	synthetic	synthetic	JJ
cord-021063-4y8m33ea	43	35	construct	construct	NN
cord-021063-4y8m33ea	43	36	,	,	,
cord-021063-4y8m33ea	43	37	and	and	CC
cord-021063-4y8m33ea	43	38	(	(	-LRB-
cord-021063-4y8m33ea	43	39	iii	iii	NN
cord-021063-4y8m33ea	43	40	)	)	-RRB-
cord-021063-4y8m33ea	43	41	artificial	artificial	JJ
cord-021063-4y8m33ea	43	42	delivery	delivery	NN
cord-021063-4y8m33ea	43	43	systems	system	NNS
cord-021063-4y8m33ea	43	44	.	.	.
cord-021063-4y8m33ea	44	1	Given	give	VBN
cord-021063-4y8m33ea	44	2	the	the	DT
cord-021063-4y8m33ea	44	3	amount	amount	NN
cord-021063-4y8m33ea	44	4	of	of	IN
cord-021063-4y8m33ea	44	5	effort	effort	NN
cord-021063-4y8m33ea	44	6	being	be	VBG
cord-021063-4y8m33ea	44	7	expended	expend	VBN
cord-021063-4y8m33ea	44	8	on	on	IN
cord-021063-4y8m33ea	44	9	the	the	DT
cord-021063-4y8m33ea	44	10	development	development	NN
cord-021063-4y8m33ea	44	11	of	of	IN
cord-021063-4y8m33ea	44	12	these	these	DT
cord-021063-4y8m33ea	44	13	systems	system	NNS
cord-021063-4y8m33ea	44	14	,	,	,
cord-021063-4y8m33ea	44	15	and	and	CC
cord-021063-4y8m33ea	44	16	the	the	DT
cord-021063-4y8m33ea	44	17	highly	highly	RB
cord-021063-4y8m33ea	44	18	varied	varied	JJ
cord-021063-4y8m33ea	44	19	nature	nature	NN
cord-021063-4y8m33ea	44	20	of	of	IN
cord-021063-4y8m33ea	44	21	the	the	DT
cord-021063-4y8m33ea	44	22	intended	intend	VBN
cord-021063-4y8m33ea	44	23	target	target	NN
cord-021063-4y8m33ea	44	24	diseases	disease	NNS
cord-021063-4y8m33ea	44	25	and	and	CC
cord-021063-4y8m33ea	44	26	organs	organ	NNS
cord-021063-4y8m33ea	44	27	,	,	,
cord-021063-4y8m33ea	44	28	it	-PRON-	PRP
cord-021063-4y8m33ea	44	29	is	be	VBZ
cord-021063-4y8m33ea	44	30	likely	likely	JJ
cord-021063-4y8m33ea	44	31	that	that	IN
cord-021063-4y8m33ea	44	32	there	there	EX
cord-021063-4y8m33ea	44	33	will	will	MD
cord-021063-4y8m33ea	44	34	never	never	RB
cord-021063-4y8m33ea	44	35	be	be	VB
cord-021063-4y8m33ea	44	36	a	a	DT
cord-021063-4y8m33ea	44	37	single	single	JJ
cord-021063-4y8m33ea	44	38	best	good	JJS
cord-021063-4y8m33ea	44	39	choice	choice	NN
cord-021063-4y8m33ea	44	40	of	of	IN
cord-021063-4y8m33ea	44	41	method	method	NN
cord-021063-4y8m33ea	44	42	.	.	.
cord-021063-4y8m33ea	45	1	Every	every	DT
cord-021063-4y8m33ea	45	2	disease	disease	NN
cord-021063-4y8m33ea	45	3	will	will	MD
cord-021063-4y8m33ea	45	4	call	call	VB
cord-021063-4y8m33ea	45	5	for	for	IN
cord-021063-4y8m33ea	45	6	a	a	DT
cord-021063-4y8m33ea	45	7	tailored	tailor	VBN
cord-021063-4y8m33ea	45	8	strategy	strategy	NN
cord-021063-4y8m33ea	45	9	that	that	WDT
cord-021063-4y8m33ea	45	10	can	can	MD
cord-021063-4y8m33ea	45	11	be	be	VB
cord-021063-4y8m33ea	45	12	optimized	optimize	VBN
cord-021063-4y8m33ea	45	13	to	to	IN
cord-021063-4y8m33ea	45	14	best	good	JJS
cord-021063-4y8m33ea	45	15	fit	fit	VB
cord-021063-4y8m33ea	45	16	the	the	DT
cord-021063-4y8m33ea	45	17	needs	need	NNS
cord-021063-4y8m33ea	45	18	of	of	IN
cord-021063-4y8m33ea	45	19	the	the	DT
cord-021063-4y8m33ea	45	20	situation	situation	NN
cord-021063-4y8m33ea	45	21	.	.	.
cord-021063-4y8m33ea	46	1	Table	table	NN
cord-021063-4y8m33ea	46	2	1	1	CD
cord-021063-4y8m33ea	46	3	contains	contain	VBZ
cord-021063-4y8m33ea	46	4	a	a	DT
cord-021063-4y8m33ea	46	5	summary	summary	NN
cord-021063-4y8m33ea	46	6	and	and	CC
cord-021063-4y8m33ea	46	7	comparison	comparison	NN
cord-021063-4y8m33ea	46	8	of	of	IN
cord-021063-4y8m33ea	46	9	various	various	JJ
cord-021063-4y8m33ea	46	10	gene	gene	NN
cord-021063-4y8m33ea	46	11	therapy	therapy	NN
cord-021063-4y8m33ea	46	12	delivery	delivery	NN
cord-021063-4y8m33ea	46	13	vectors	vector	NNS
cord-021063-4y8m33ea	46	14	.	.	.
cord-021063-4y8m33ea	47	1	Ten	ten	CD
cord-021063-4y8m33ea	47	2	years	year	NNS
cord-021063-4y8m33ea	47	3	ago	ago	RB
cord-021063-4y8m33ea	47	4	,	,	,
cord-021063-4y8m33ea	47	5	it	-PRON-	PRP
cord-021063-4y8m33ea	47	6	seemed	seem	VBD
cord-021063-4y8m33ea	47	7	a	a	DT
cord-021063-4y8m33ea	47	8	foregone	foregone	JJ
cord-021063-4y8m33ea	47	9	conclusion	conclusion	NN
cord-021063-4y8m33ea	47	10	that	that	IN
cord-021063-4y8m33ea	47	11	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	47	12	were	be	VBD
cord-021063-4y8m33ea	47	13	the	the	DT
cord-021063-4y8m33ea	47	14	only	only	JJ
cord-021063-4y8m33ea	47	15	serious	serious	JJ
cord-021063-4y8m33ea	47	16	candidate	candidate	NN
cord-021063-4y8m33ea	47	17	for	for	IN
cord-021063-4y8m33ea	47	18	an	an	DT
cord-021063-4y8m33ea	47	19	eventual	eventual	JJ
cord-021063-4y8m33ea	47	20	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	47	21	vector	vector	NN
cord-021063-4y8m33ea	47	22	.	.	.
cord-021063-4y8m33ea	48	1	Today	today	NN
cord-021063-4y8m33ea	48	2	,	,	,
cord-021063-4y8m33ea	48	3	many	many	JJ
cord-021063-4y8m33ea	48	4	different	different	JJ
cord-021063-4y8m33ea	48	5	viral	viral	JJ
cord-021063-4y8m33ea	48	6	vectors	vector	NNS
cord-021063-4y8m33ea	48	7	are	be	VBP
cord-021063-4y8m33ea	48	8	being	be	VBG
cord-021063-4y8m33ea	48	9	considered	consider	VBN
cord-021063-4y8m33ea	48	10	for	for	IN
cord-021063-4y8m33ea	48	11	use	use	NN
cord-021063-4y8m33ea	48	12	in	in	IN
cord-021063-4y8m33ea	48	13	gene	gene	NN
cord-021063-4y8m33ea	48	14	therapy	therapy	NN
cord-021063-4y8m33ea	48	15	.	.	.
cord-021063-4y8m33ea	49	1	Because	because	IN
cord-021063-4y8m33ea	49	2	different	different	JJ
cord-021063-4y8m33ea	49	3	viruses	virus	NNS
cord-021063-4y8m33ea	49	4	target	target	VBP
cord-021063-4y8m33ea	49	5	different	different	JJ
cord-021063-4y8m33ea	49	6	cell	cell	NN
cord-021063-4y8m33ea	49	7	types	type	NNS
cord-021063-4y8m33ea	49	8	and	and	CC
cord-021063-4y8m33ea	49	9	have	have	VBP
cord-021063-4y8m33ea	49	10	different	different	JJ
cord-021063-4y8m33ea	49	11	modes	mode	NNS
cord-021063-4y8m33ea	49	12	of	of	IN
cord-021063-4y8m33ea	49	13	replication	replication	NN
cord-021063-4y8m33ea	49	14	,	,	,
cord-021063-4y8m33ea	49	15	each	each	DT
cord-021063-4y8m33ea	49	16	viral	viral	JJ
cord-021063-4y8m33ea	49	17	delivery	delivery	NN
cord-021063-4y8m33ea	49	18	system	system	NN
cord-021063-4y8m33ea	49	19	may	may	MD
cord-021063-4y8m33ea	49	20	be	be	VB
cord-021063-4y8m33ea	49	21	best	well	RBS
cord-021063-4y8m33ea	49	22	suited	suit	VBN
cord-021063-4y8m33ea	49	23	for	for	IN
cord-021063-4y8m33ea	49	24	treatment	treatment	NN
cord-021063-4y8m33ea	49	25	of	of	IN
cord-021063-4y8m33ea	49	26	different	different	JJ
cord-021063-4y8m33ea	49	27	diseases	disease	NNS
cord-021063-4y8m33ea	49	28	.	.	.
cord-021063-4y8m33ea	50	1	The	the	DT
cord-021063-4y8m33ea	50	2	viral	viral	JJ
cord-021063-4y8m33ea	50	3	vectors	vector	NNS
cord-021063-4y8m33ea	50	4	under	under	IN
cord-021063-4y8m33ea	50	5	most	most	JJS
cord-021063-4y8m33ea	50	6	active	active	JJ
cord-021063-4y8m33ea	50	7	development	development	NN
cord-021063-4y8m33ea	50	8	are	be	VBP
cord-021063-4y8m33ea	50	9	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	50	10	,	,	,
cord-021063-4y8m33ea	50	11	adenoviruses	adenovirus	NNS
cord-021063-4y8m33ea	50	12	,	,	,
cord-021063-4y8m33ea	50	13	adeno	adeno	NN
cord-021063-4y8m33ea	50	14	-	-	HYPH
cord-021063-4y8m33ea	50	15	associated	associate	VBN
cord-021063-4y8m33ea	50	16	virus	virus	NN
cord-021063-4y8m33ea	50	17	,	,	,
cord-021063-4y8m33ea	50	18	and	and	CC
cord-021063-4y8m33ea	50	19	herpesviruses	herpesviruses	NNP
cord-021063-4y8m33ea	50	20	.	.	.
cord-021063-4y8m33ea	51	1	Some	some	DT
cord-021063-4y8m33ea	51	2	general	general	JJ
cord-021063-4y8m33ea	51	3	characteristics	characteristic	NNS
cord-021063-4y8m33ea	51	4	of	of	IN
cord-021063-4y8m33ea	51	5	the	the	DT
cord-021063-4y8m33ea	51	6	viruses	virus	NNS
cord-021063-4y8m33ea	51	7	currently	currently	RB
cord-021063-4y8m33ea	51	8	being	be	VBG
cord-021063-4y8m33ea	51	9	developed	develop	VBN
cord-021063-4y8m33ea	51	10	as	as	IN
cord-021063-4y8m33ea	51	11	gene	gene	NN
cord-021063-4y8m33ea	51	12	therapy	therapy	NN
cord-021063-4y8m33ea	51	13	vectors	vector	NNS
cord-021063-4y8m33ea	51	14	are	be	VBP
cord-021063-4y8m33ea	51	15	presented	present	VBN
cord-021063-4y8m33ea	51	16	in	in	IN
cord-021063-4y8m33ea	51	17	Table	Table	NNP
cord-021063-4y8m33ea	51	18	2	2	CD
cord-021063-4y8m33ea	51	19	.	.	.
cord-021063-4y8m33ea	52	1	Wild	wild	JJ
cord-021063-4y8m33ea	52	2	-	-	HYPH
cord-021063-4y8m33ea	52	3	type	type	NN
cord-021063-4y8m33ea	52	4	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	52	5	are	be	VBP
cord-021063-4y8m33ea	52	6	contained	contain	VBN
cord-021063-4y8m33ea	52	7	within	within	IN
cord-021063-4y8m33ea	52	8	a	a	DT
cord-021063-4y8m33ea	52	9	lipid	lipid	NN
cord-021063-4y8m33ea	52	10	membrane	membrane	NN
cord-021063-4y8m33ea	52	11	and	and	CC
cord-021063-4y8m33ea	52	12	have	have	VBP
cord-021063-4y8m33ea	52	13	two	two	CD
cord-021063-4y8m33ea	52	14	identical	identical	JJ
cord-021063-4y8m33ea	52	15	copies	copy	NNS
cord-021063-4y8m33ea	52	16	of	of	IN
cord-021063-4y8m33ea	52	17	an	an	DT
cord-021063-4y8m33ea	52	18	RNA	RNA	NNP
cord-021063-4y8m33ea	52	19	genome	genome	NN
cord-021063-4y8m33ea	52	20	.	.	.
cord-021063-4y8m33ea	53	1	After	after	IN
cord-021063-4y8m33ea	53	2	the	the	DT
cord-021063-4y8m33ea	53	3	viral	viral	JJ
cord-021063-4y8m33ea	53	4	particle	particle	NN
cord-021063-4y8m33ea	53	5	's	's	POS
cord-021063-4y8m33ea	53	6	entry	entry	NN
cord-021063-4y8m33ea	53	7	into	into	IN
cord-021063-4y8m33ea	53	8	the	the	DT
cord-021063-4y8m33ea	53	9	cell	cell	NN
cord-021063-4y8m33ea	53	10	,	,	,
cord-021063-4y8m33ea	53	11	the	the	DT
cord-021063-4y8m33ea	53	12	RNA	RNA	NNP
cord-021063-4y8m33ea	53	13	molecule	molecule	NN
cord-021063-4y8m33ea	53	14	is	be	VBZ
cord-021063-4y8m33ea	53	15	reverse	reverse	RB
cord-021063-4y8m33ea	53	16	-	-	HYPH
cord-021063-4y8m33ea	53	17	transcribed	transcribe	VBN
cord-021063-4y8m33ea	53	18	into	into	IN
cord-021063-4y8m33ea	53	19	a	a	DT
cord-021063-4y8m33ea	53	20	DNA	dna	NN
cord-021063-4y8m33ea	53	21	copy	copy	NN
cord-021063-4y8m33ea	53	22	,	,	,
cord-021063-4y8m33ea	53	23	which	which	WDT
cord-021063-4y8m33ea	53	24	is	be	VBZ
cord-021063-4y8m33ea	53	25	then	then	RB
cord-021063-4y8m33ea	53	26	integrated	integrate	VBN
cord-021063-4y8m33ea	53	27	into	into	IN
cord-021063-4y8m33ea	53	28	the	the	DT
cord-021063-4y8m33ea	53	29	host	host	NN
cord-021063-4y8m33ea	53	30	's	's	POS
cord-021063-4y8m33ea	53	31	genome	genome	NN
cord-021063-4y8m33ea	53	32	,	,	,
cord-021063-4y8m33ea	53	33	mRNAs	mRNAs	NNPS
cord-021063-4y8m33ea	53	34	for	for	IN
cord-021063-4y8m33ea	53	35	the	the	DT
cord-021063-4y8m33ea	53	36	various	various	JJ
cord-021063-4y8m33ea	53	37	viral	viral	JJ
cord-021063-4y8m33ea	53	38	proteins	protein	NNS
cord-021063-4y8m33ea	53	39	synthesized	synthesize	VBN
cord-021063-4y8m33ea	53	40	during	during	IN
cord-021063-4y8m33ea	53	41	the	the	DT
cord-021063-4y8m33ea	53	42	course	course	NN
cord-021063-4y8m33ea	53	43	of	of	IN
cord-021063-4y8m33ea	53	44	the	the	DT
cord-021063-4y8m33ea	53	45	infection	infection	NN
cord-021063-4y8m33ea	53	46	are	be	VBP
cord-021063-4y8m33ea	53	47	transcribed	transcribe	VBN
cord-021063-4y8m33ea	53	48	from	from	IN
cord-021063-4y8m33ea	53	49	this	this	DT
cord-021063-4y8m33ea	53	50	integrated	integrate	VBN
cord-021063-4y8m33ea	53	51	DNA	dna	NN
cord-021063-4y8m33ea	53	52	.	.	.
cord-021063-4y8m33ea	54	1	The	the	DT
cord-021063-4y8m33ea	54	2	integration	integration	NN
cord-021063-4y8m33ea	54	3	of	of	IN
cord-021063-4y8m33ea	54	4	the	the	DT
cord-021063-4y8m33ea	54	5	retroviral	retroviral	JJ
cord-021063-4y8m33ea	54	6	DNA	dna	NN
cord-021063-4y8m33ea	54	7	genome	genome	NN
cord-021063-4y8m33ea	54	8	into	into	IN
cord-021063-4y8m33ea	54	9	the	the	DT
cord-021063-4y8m33ea	54	10	host	host	NN
cord-021063-4y8m33ea	54	11	genome	genome	NN
cord-021063-4y8m33ea	54	12	forms	form	VBZ
cord-021063-4y8m33ea	54	13	the	the	DT
cord-021063-4y8m33ea	54	14	basis	basis	NN
cord-021063-4y8m33ea	54	15	for	for	IN
cord-021063-4y8m33ea	54	16	the	the	DT
cord-021063-4y8m33ea	54	17	use	use	NN
cord-021063-4y8m33ea	54	18	of	of	IN
cord-021063-4y8m33ea	54	19	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	54	20	as	as	IN
cord-021063-4y8m33ea	54	21	vectors	vector	NNS
cord-021063-4y8m33ea	54	22	for	for	IN
cord-021063-4y8m33ea	54	23	the	the	DT
cord-021063-4y8m33ea	54	24	introduction	introduction	NN
cord-021063-4y8m33ea	54	25	of	of	IN
cord-021063-4y8m33ea	54	26	exogenous	exogenous	JJ
cord-021063-4y8m33ea	54	27	DNA	dna	NN
cord-021063-4y8m33ea	54	28	into	into	IN
cord-021063-4y8m33ea	54	29	eukaryotic	eukaryotic	JJ
cord-021063-4y8m33ea	54	30	cells	cell	NNS
cord-021063-4y8m33ea	55	1	(	(	-LRB-
cord-021063-4y8m33ea	55	2	Hoeben	Hoeben	NNP
cord-021063-4y8m33ea	55	3	et	et	FW
cord-021063-4y8m33ea	55	4	al	al	NNP
cord-021063-4y8m33ea	55	5	.	.	NNP
cord-021063-4y8m33ea	55	6	,	,	,
cord-021063-4y8m33ea	55	7	1992	1992	CD
cord-021063-4y8m33ea	55	8	)	)	-RRB-
cord-021063-4y8m33ea	55	9	.	.	.
cord-021063-4y8m33ea	56	1	To	to	TO
cord-021063-4y8m33ea	56	2	convert	convert	VB
cord-021063-4y8m33ea	56	3	a	a	DT
cord-021063-4y8m33ea	56	4	wild	wild	JJ
cord-021063-4y8m33ea	56	5	-	-	HYPH
cord-021063-4y8m33ea	56	6	type	type	NN
cord-021063-4y8m33ea	56	7	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	56	8	into	into	IN
cord-021063-4y8m33ea	56	9	a	a	DT
cord-021063-4y8m33ea	56	10	retroviral	retroviral	JJ
cord-021063-4y8m33ea	56	11	gene	gene	NN
cord-021063-4y8m33ea	56	12	therapy	therapy	NN
cord-021063-4y8m33ea	56	13	vector	vector	NN
cord-021063-4y8m33ea	56	14	,	,	,
cord-021063-4y8m33ea	56	15	two	two	CD
cord-021063-4y8m33ea	56	16	steps	step	NNS
cord-021063-4y8m33ea	56	17	are	be	VBP
cord-021063-4y8m33ea	56	18	necessary	necessary	JJ
cord-021063-4y8m33ea	56	19	:	:	:
cord-021063-4y8m33ea	56	20	first	first	RB
cord-021063-4y8m33ea	56	21	,	,	,
cord-021063-4y8m33ea	56	22	the	the	DT
cord-021063-4y8m33ea	56	23	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	56	24	gene	gene	NN
cord-021063-4y8m33ea	56	25	must	must	MD
cord-021063-4y8m33ea	56	26	be	be	VB
cord-021063-4y8m33ea	56	27	added	add	VBN
cord-021063-4y8m33ea	56	28	to	to	IN
cord-021063-4y8m33ea	56	29	the	the	DT
cord-021063-4y8m33ea	56	30	retroviral	retroviral	NN
cord-021063-4y8m33ea	56	31	and	and	CC
cord-021063-4y8m33ea	56	32	,	,	,
cord-021063-4y8m33ea	56	33	genome	genome	JJ
cord-021063-4y8m33ea	56	34	second	second	NN
cord-021063-4y8m33ea	56	35	,	,	,
cord-021063-4y8m33ea	56	36	the	the	DT
cord-021063-4y8m33ea	56	37	potential	potential	NN
cord-021063-4y8m33ea	56	38	of	of	IN
cord-021063-4y8m33ea	56	39	the	the	DT
cord-021063-4y8m33ea	56	40	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	56	41	to	to	TO
cord-021063-4y8m33ea	56	42	remain	remain	VB
cord-021063-4y8m33ea	56	43	infectious	infectious	JJ
cord-021063-4y8m33ea	56	44	after	after	IN
cord-021063-4y8m33ea	56	45	integration	integration	NN
cord-021063-4y8m33ea	56	46	into	into	IN
cord-021063-4y8m33ea	56	47	the	the	DT
cord-021063-4y8m33ea	56	48	target	target	NN
cord-021063-4y8m33ea	56	49	cell	cell	NN
cord-021063-4y8m33ea	56	50	must	must	MD
cord-021063-4y8m33ea	56	51	be	be	VB
cord-021063-4y8m33ea	56	52	eliminated	eliminate	VBN
cord-021063-4y8m33ea	56	53	.	.	.
cord-021063-4y8m33ea	57	1	Presently	presently	RB
cord-021063-4y8m33ea	57	2	,	,	,
cord-021063-4y8m33ea	57	3	this	this	DT
cord-021063-4y8m33ea	57	4	is	be	VBZ
cord-021063-4y8m33ea	57	5	accomplished	accomplish	VBN
cord-021063-4y8m33ea	57	6	by	by	IN
cord-021063-4y8m33ea	57	7	inserting	insert	VBG
cord-021063-4y8m33ea	57	8	a	a	DT
cord-021063-4y8m33ea	57	9	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	57	10	gene	gene	NN
cord-021063-4y8m33ea	57	11	into	into	IN
cord-021063-4y8m33ea	57	12	a	a	DT
cord-021063-4y8m33ea	57	13	retroviral	retroviral	JJ
cord-021063-4y8m33ea	57	14	genome	genome	NN
cord-021063-4y8m33ea	57	15	that	that	WDT
cord-021063-4y8m33ea	57	16	has	have	VBZ
cord-021063-4y8m33ea	57	17	had	have	VBN
cord-021063-4y8m33ea	57	18	most	most	JJS
cord-021063-4y8m33ea	57	19	of	of	IN
cord-021063-4y8m33ea	57	20	the	the	DT
cord-021063-4y8m33ea	57	21	genes	gene	NNS
cord-021063-4y8m33ea	57	22	necessary	necessary	JJ
cord-021063-4y8m33ea	57	23	for	for	IN
cord-021063-4y8m33ea	57	24	packaging	package	VBG
cord-021063-4y8m33ea	57	25	the	the	DT
cord-021063-4y8m33ea	57	26	virus	virus	NN
cord-021063-4y8m33ea	57	27	removed	remove	VBN
cord-021063-4y8m33ea	57	28	or	or	CC
cord-021063-4y8m33ea	57	29	mutated	mutate	VBN
cord-021063-4y8m33ea	57	30	.	.	.
cord-021063-4y8m33ea	58	1	Deletion	deletion	NN
cord-021063-4y8m33ea	58	2	of	of	IN
cord-021063-4y8m33ea	58	3	the	the	DT
cord-021063-4y8m33ea	58	4	viral	viral	JJ
cord-021063-4y8m33ea	58	5	genes	gene	NNS
cord-021063-4y8m33ea	58	6	eliminates	eliminate	VBZ
cord-021063-4y8m33ea	58	7	infectivity	infectivity	NN
cord-021063-4y8m33ea	58	8	in	in	IN
cord-021063-4y8m33ea	58	9	the	the	DT
cord-021063-4y8m33ea	58	10	treated	treat	VBN
cord-021063-4y8m33ea	58	11	cells	cell	NNS
cord-021063-4y8m33ea	58	12	by	by	IN
cord-021063-4y8m33ea	58	13	preventing	prevent	VBG
cord-021063-4y8m33ea	58	14	the	the	DT
cord-021063-4y8m33ea	58	15	newly	newly	RB
cord-021063-4y8m33ea	58	16	integrated	integrate	VBN
cord-021063-4y8m33ea	58	17	genome	genome	NN
cord-021063-4y8m33ea	58	18	from	from	IN
cord-021063-4y8m33ea	58	19	making	make	VBG
cord-021063-4y8m33ea	58	20	infectious	infectious	JJ
cord-021063-4y8m33ea	58	21	copies	copy	NNS
cord-021063-4y8m33ea	58	22	of	of	IN
cord-021063-4y8m33ea	58	23	itself	-PRON-	PRP
cord-021063-4y8m33ea	58	24	that	that	WDT
cord-021063-4y8m33ea	58	25	could	could	MD
cord-021063-4y8m33ea	58	26	complete	complete	VB
cord-021063-4y8m33ea	58	27	further	further	JJ
cord-021063-4y8m33ea	58	28	rounds	round	NNS
cord-021063-4y8m33ea	58	29	of	of	IN
cord-021063-4y8m33ea	58	30	infection	infection	NN
cord-021063-4y8m33ea	58	31	.	.	.
cord-021063-4y8m33ea	59	1	It	-PRON-	PRP
cord-021063-4y8m33ea	59	2	also	also	RB
cord-021063-4y8m33ea	59	3	has	have	VBZ
cord-021063-4y8m33ea	59	4	the	the	DT
cord-021063-4y8m33ea	59	5	added	add	VBN
cord-021063-4y8m33ea	59	6	benefit	benefit	NN
cord-021063-4y8m33ea	59	7	of	of	IN
cord-021063-4y8m33ea	59	8	increasing	increase	VBG
cord-021063-4y8m33ea	59	9	the	the	DT
cord-021063-4y8m33ea	59	10	maximum	maximum	JJ
cord-021063-4y8m33ea	59	11	length	length	NN
cord-021063-4y8m33ea	59	12	of	of	IN
cord-021063-4y8m33ea	59	13	a	a	DT
cord-021063-4y8m33ea	59	14	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	59	15	gene	gene	NN
cord-021063-4y8m33ea	59	16	that	that	WDT
cord-021063-4y8m33ea	59	17	can	can	MD
cord-021063-4y8m33ea	59	18	be	be	VB
cord-021063-4y8m33ea	59	19	delivered	deliver	VBN
cord-021063-4y8m33ea	59	20	by	by	IN
cord-021063-4y8m33ea	59	21	a	a	DT
cord-021063-4y8m33ea	59	22	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	59	23	.	.	.
cord-021063-4y8m33ea	60	1	Some	some	DT
cord-021063-4y8m33ea	60	2	advantages	advantage	NNS
cord-021063-4y8m33ea	60	3	of	of	IN
cord-021063-4y8m33ea	60	4	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	60	5	as	as	IN
cord-021063-4y8m33ea	60	6	gene	gene	NN
cord-021063-4y8m33ea	60	7	delivery	delivery	NN
cord-021063-4y8m33ea	60	8	vectors	vector	NNS
cord-021063-4y8m33ea	60	9	include	include	VBP
cord-021063-4y8m33ea	60	10	:	:	:
cord-021063-4y8m33ea	60	11	(	(	-LRB-
cord-021063-4y8m33ea	60	12	i	i	NN
cord-021063-4y8m33ea	60	13	)	)	-RRB-
cord-021063-4y8m33ea	61	1	the	the	DT
cord-021063-4y8m33ea	61	2	efficient	efficient	JJ
cord-021063-4y8m33ea	61	3	and	and	CC
cord-021063-4y8m33ea	61	4	stable	stable	JJ
cord-021063-4y8m33ea	61	5	integration	integration	NN
cord-021063-4y8m33ea	61	6	of	of	IN
cord-021063-4y8m33ea	61	7	the	the	DT
cord-021063-4y8m33ea	61	8	introduced	introduce	VBN
cord-021063-4y8m33ea	61	9	gene	gene	NN
cord-021063-4y8m33ea	61	10	into	into	IN
cord-021063-4y8m33ea	61	11	the	the	DT
cord-021063-4y8m33ea	61	12	host	host	NN
cord-021063-4y8m33ea	61	13	genome	genome	NN
cord-021063-4y8m33ea	61	14	,	,	,
cord-021063-4y8m33ea	61	15	(	(	-LRB-
cord-021063-4y8m33ea	61	16	ii	ii	NN
cord-021063-4y8m33ea	61	17	)	)	-RRB-
cord-021063-4y8m33ea	61	18	a	a	DT
cord-021063-4y8m33ea	61	19	wide	wide	JJ
cord-021063-4y8m33ea	61	20	host	host	NN
cord-021063-4y8m33ea	61	21	range	range	NN
cord-021063-4y8m33ea	61	22	,	,	,
cord-021063-4y8m33ea	61	23	and	and	CC
cord-021063-4y8m33ea	61	24	(	(	-LRB-
cord-021063-4y8m33ea	61	25	iii	iii	NN
cord-021063-4y8m33ea	61	26	)	)	-RRB-
cord-021063-4y8m33ea	62	1	the	the	DT
cord-021063-4y8m33ea	62	2	ability	ability	NN
cord-021063-4y8m33ea	62	3	to	to	TO
cord-021063-4y8m33ea	62	4	infect	infect	VB
cord-021063-4y8m33ea	62	5	large	large	JJ
cord-021063-4y8m33ea	62	6	numbers	number	NNS
cord-021063-4y8m33ea	62	7	of	of	IN
cord-021063-4y8m33ea	62	8	cells	cell	NNS
cord-021063-4y8m33ea	62	9	.	.	.
cord-021063-4y8m33ea	63	1	Potential	potential	JJ
cord-021063-4y8m33ea	63	2	problems	problem	NNS
cord-021063-4y8m33ea	63	3	associated	associate	VBN
cord-021063-4y8m33ea	63	4	with	with	IN
cord-021063-4y8m33ea	63	5	the	the	DT
cord-021063-4y8m33ea	63	6	use	use	NN
cord-021063-4y8m33ea	63	7	of	of	IN
cord-021063-4y8m33ea	63	8	viral	viral	JJ
cord-021063-4y8m33ea	63	9	vectors	vector	NNS
cord-021063-4y8m33ea	63	10	include	include	VBP
cord-021063-4y8m33ea	63	11	:	:	:
cord-021063-4y8m33ea	63	12	(	(	-LRB-
cord-021063-4y8m33ea	63	13	i	i	NN
cord-021063-4y8m33ea	63	14	)	)	-RRB-
cord-021063-4y8m33ea	64	1	the	the	DT
cord-021063-4y8m33ea	64	2	possibility	possibility	NN
cord-021063-4y8m33ea	64	3	of	of	IN
cord-021063-4y8m33ea	64	4	recombination	recombination	NN
cord-021063-4y8m33ea	64	5	events	event	NNS
cord-021063-4y8m33ea	64	6	that	that	WDT
cord-021063-4y8m33ea	64	7	could	could	MD
cord-021063-4y8m33ea	64	8	convert	convert	VB
cord-021063-4y8m33ea	64	9	a	a	DT
cord-021063-4y8m33ea	64	10	replication	replication	NN
cord-021063-4y8m33ea	64	11	-	-	HYPH
cord-021063-4y8m33ea	64	12	defective	defective	JJ
cord-021063-4y8m33ea	64	13	vector	vector	NN
cord-021063-4y8m33ea	64	14	into	into	IN
cord-021063-4y8m33ea	64	15	an	an	DT
cord-021063-4y8m33ea	64	16	infectious	infectious	JJ
cord-021063-4y8m33ea	64	17	agent	agent	NN
cord-021063-4y8m33ea	64	18	,	,	,
cord-021063-4y8m33ea	64	19	(	(	-LRB-
cord-021063-4y8m33ea	64	20	ii	ii	NN
cord-021063-4y8m33ea	64	21	)	)	-RRB-
cord-021063-4y8m33ea	65	1	the	the	DT
cord-021063-4y8m33ea	65	2	possibility	possibility	NN
cord-021063-4y8m33ea	65	3	that	that	IN
cord-021063-4y8m33ea	65	4	superinfection	superinfection	NN
cord-021063-4y8m33ea	65	5	with	with	IN
cord-021063-4y8m33ea	65	6	another	another	DT
cord-021063-4y8m33ea	65	7	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	65	8	may	may	MD
cord-021063-4y8m33ea	65	9	allow	allow	VB
cord-021063-4y8m33ea	65	10	unwanted	unwanted	JJ
cord-021063-4y8m33ea	65	11	transfer	transfer	NN
cord-021063-4y8m33ea	65	12	of	of	IN
cord-021063-4y8m33ea	65	13	the	the	DT
cord-021063-4y8m33ea	65	14	introduced	introduce	VBN
cord-021063-4y8m33ea	65	15	gene	gene	NN
cord-021063-4y8m33ea	65	16	between	between	IN
cord-021063-4y8m33ea	65	17	individuals	individual	NNS
cord-021063-4y8m33ea	65	18	,	,	,
cord-021063-4y8m33ea	65	19	(	(	-LRB-
cord-021063-4y8m33ea	65	20	iii	iii	NN
cord-021063-4y8m33ea	65	21	)	)	-RRB-
cord-021063-4y8m33ea	65	22	a	a	DT
cord-021063-4y8m33ea	65	23	7	7	CD
cord-021063-4y8m33ea	65	24	-	-	SYM
cord-021063-4y8m33ea	65	25	13	13	CD
cord-021063-4y8m33ea	65	26	kb	kb	NNP
cord-021063-4y8m33ea	65	27	limit	limit	NN
cord-021063-4y8m33ea	65	28	on	on	IN
cord-021063-4y8m33ea	65	29	the	the	DT
cord-021063-4y8m33ea	65	30	amount	amount	NN
cord-021063-4y8m33ea	65	31	of	of	IN
cord-021063-4y8m33ea	65	32	DNA	dna	NN
cord-021063-4y8m33ea	65	33	that	that	WDT
cord-021063-4y8m33ea	65	34	can	can	MD
cord-021063-4y8m33ea	65	35	be	be	VB
cord-021063-4y8m33ea	65	36	packaged	package	VBN
cord-021063-4y8m33ea	65	37	,	,	,
cord-021063-4y8m33ea	65	38	(	(	-LRB-
cord-021063-4y8m33ea	65	39	iv	iv	LS
cord-021063-4y8m33ea	65	40	)	)	-RRB-
cord-021063-4y8m33ea	65	41	potential	potential	JJ
cord-021063-4y8m33ea	65	42	problems	problem	NNS
cord-021063-4y8m33ea	65	43	in	in	IN
cord-021063-4y8m33ea	65	44	targeting	target	VBG
cord-021063-4y8m33ea	65	45	the	the	DT
cord-021063-4y8m33ea	65	46	virus	virus	NN
cord-021063-4y8m33ea	65	47	to	to	IN
cord-021063-4y8m33ea	65	48	specific	specific	JJ
cord-021063-4y8m33ea	65	49	cells	cell	NNS
cord-021063-4y8m33ea	65	50	,	,	,
cord-021063-4y8m33ea	65	51	and	and	CC
cord-021063-4y8m33ea	65	52	(	(	-LRB-
cord-021063-4y8m33ea	65	53	v	v	NN
cord-021063-4y8m33ea	65	54	)	)	-RRB-
cord-021063-4y8m33ea	65	55	difficulty	difficulty	NN
cord-021063-4y8m33ea	65	56	in	in	IN
cord-021063-4y8m33ea	65	57	maintaining	maintain	VBG
cord-021063-4y8m33ea	65	58	high	high	JJ
cord-021063-4y8m33ea	65	59	-	-	HYPH
cord-021063-4y8m33ea	65	60	level	level	NN
cord-021063-4y8m33ea	65	61	expression	expression	NN
cord-021063-4y8m33ea	65	62	of	of	IN
cord-021063-4y8m33ea	65	63	the	the	DT
cord-021063-4y8m33ea	65	64	exogenous	exogenous	JJ
cord-021063-4y8m33ea	65	65	gene	gene	NN
cord-021063-4y8m33ea	65	66	.	.	.
cord-021063-4y8m33ea	66	1	Wild	wild	JJ
cord-021063-4y8m33ea	66	2	-	-	HYPH
cord-021063-4y8m33ea	66	3	type	type	NN
cord-021063-4y8m33ea	66	4	adenoviruses	adenovirus	NNS
cord-021063-4y8m33ea	66	5	cause	cause	VBP
cord-021063-4y8m33ea	66	6	respiratory	respiratory	JJ
cord-021063-4y8m33ea	66	7	disease	disease	NN
cord-021063-4y8m33ea	66	8	in	in	IN
cord-021063-4y8m33ea	66	9	humans	human	NNS
cord-021063-4y8m33ea	66	10	.	.	.
cord-021063-4y8m33ea	67	1	The	the	DT
cord-021063-4y8m33ea	67	2	adenovirus	adenovirus	NN
cord-021063-4y8m33ea	67	3	genome	genome	NN
cord-021063-4y8m33ea	67	4	is	be	VBZ
cord-021063-4y8m33ea	67	5	a	a	DT
cord-021063-4y8m33ea	67	6	linear	linear	JJ
cord-021063-4y8m33ea	67	7	double	double	RB
cord-021063-4y8m33ea	67	8	-	-	HYPH
cord-021063-4y8m33ea	67	9	stranded	strand	VBN
cord-021063-4y8m33ea	67	10	DNA	dna	NN
cord-021063-4y8m33ea	67	11	molecule	molecule	NN
cord-021063-4y8m33ea	67	12	,	,	,
cord-021063-4y8m33ea	67	13	about	about	RB
cord-021063-4y8m33ea	67	14	36	36	CD
cord-021063-4y8m33ea	67	15	kb	kb	NNS
cord-021063-4y8m33ea	67	16	in	in	IN
cord-021063-4y8m33ea	67	17	length	length	NN
cord-021063-4y8m33ea	67	18	.	.	.
cord-021063-4y8m33ea	68	1	Although	although	IN
cord-021063-4y8m33ea	68	2	the	the	DT
cord-021063-4y8m33ea	68	3	virus	virus	NN
cord-021063-4y8m33ea	68	4	does	do	VBZ
cord-021063-4y8m33ea	68	5	not	not	RB
cord-021063-4y8m33ea	68	6	have	have	VB
cord-021063-4y8m33ea	68	7	a	a	DT
cord-021063-4y8m33ea	68	8	lipid	lipid	NN
cord-021063-4y8m33ea	68	9	coat	coat	NN
cord-021063-4y8m33ea	68	10	,	,	,
cord-021063-4y8m33ea	68	11	it	-PRON-	PRP
cord-021063-4y8m33ea	68	12	is	be	VBZ
cord-021063-4y8m33ea	68	13	taken	take	VBN
cord-021063-4y8m33ea	68	14	up	up	RP
cord-021063-4y8m33ea	68	15	by	by	IN
cord-021063-4y8m33ea	68	16	cells	cell	NNS
cord-021063-4y8m33ea	68	17	via	via	IN
cord-021063-4y8m33ea	68	18	endocytosis	endocytosis	NN
cord-021063-4y8m33ea	68	19	and	and	CC
cord-021063-4y8m33ea	68	20	enters	enter	VBZ
cord-021063-4y8m33ea	68	21	the	the	DT
cord-021063-4y8m33ea	68	22	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	68	23	in	in	IN
cord-021063-4y8m33ea	68	24	a	a	DT
cord-021063-4y8m33ea	68	25	manner	manner	NN
cord-021063-4y8m33ea	68	26	analogous	analogous	JJ
cord-021063-4y8m33ea	68	27	to	to	IN
cord-021063-4y8m33ea	68	28	the	the	DT
cord-021063-4y8m33ea	68	29	retrovirus	retrovirus	NN
cord-021063-4y8m33ea	68	30	.	.	.
cord-021063-4y8m33ea	69	1	Once	once	IN
cord-021063-4y8m33ea	69	2	inside	inside	IN
cord-021063-4y8m33ea	69	3	the	the	DT
cord-021063-4y8m33ea	69	4	cell	cell	NN
cord-021063-4y8m33ea	69	5	,	,	,
cord-021063-4y8m33ea	69	6	the	the	DT
cord-021063-4y8m33ea	69	7	viral	viral	JJ
cord-021063-4y8m33ea	69	8	DNA	dna	NN
cord-021063-4y8m33ea	69	9	moves	move	NNS
cord-021063-4y8m33ea	69	10	to	to	IN
cord-021063-4y8m33ea	69	11	the	the	DT
cord-021063-4y8m33ea	69	12	nucleus	nucleus	NN
cord-021063-4y8m33ea	69	13	and	and	CC
cord-021063-4y8m33ea	69	14	begins	begin	VBZ
cord-021063-4y8m33ea	69	15	its	-PRON-	PRP$
cord-021063-4y8m33ea	69	16	replication	replication	NN
cord-021063-4y8m33ea	69	17	cycle	cycle	NN
cord-021063-4y8m33ea	69	18	without	without	IN
cord-021063-4y8m33ea	69	19	becoming	become	VBG
cord-021063-4y8m33ea	69	20	part	part	NN
cord-021063-4y8m33ea	69	21	of	of	IN
cord-021063-4y8m33ea	69	22	the	the	DT
cord-021063-4y8m33ea	69	23	host	host	NN
cord-021063-4y8m33ea	69	24	cell	cell	NN
cord-021063-4y8m33ea	69	25	's	's	POS
cord-021063-4y8m33ea	69	26	genome	genome	NN
cord-021063-4y8m33ea	69	27	.	.	.
cord-021063-4y8m33ea	70	1	Adenoviruses	adenovirus	NNS
cord-021063-4y8m33ea	70	2	that	that	WDT
cord-021063-4y8m33ea	70	3	are	be	VBP
cord-021063-4y8m33ea	70	4	being	be	VBG
cord-021063-4y8m33ea	70	5	considered	consider	VBN
cord-021063-4y8m33ea	70	6	for	for	IN
cord-021063-4y8m33ea	70	7	use	use	NN
cord-021063-4y8m33ea	70	8	as	as	IN
cord-021063-4y8m33ea	70	9	gene	gene	NN
cord-021063-4y8m33ea	70	10	therapy	therapy	NN
cord-021063-4y8m33ea	70	11	vectors	vector	NNS
cord-021063-4y8m33ea	70	12	have	have	VBP
cord-021063-4y8m33ea	70	13	had	have	VBN
cord-021063-4y8m33ea	70	14	substantial	substantial	JJ
cord-021063-4y8m33ea	70	15	parts	part	NNS
cord-021063-4y8m33ea	70	16	of	of	IN
cord-021063-4y8m33ea	70	17	their	-PRON-	PRP$
cord-021063-4y8m33ea	70	18	genome	genome	NN
cord-021063-4y8m33ea	70	19	removed	remove	VBN
cord-021063-4y8m33ea	70	20	,	,	,
cord-021063-4y8m33ea	70	21	rendering	render	VBG
cord-021063-4y8m33ea	70	22	them	-PRON-	PRP
cord-021063-4y8m33ea	70	23	unable	unable	JJ
cord-021063-4y8m33ea	70	24	to	to	TO
cord-021063-4y8m33ea	70	25	replicate	replicate	VB
cord-021063-4y8m33ea	70	26	except	except	IN
cord-021063-4y8m33ea	70	27	in	in	IN
cord-021063-4y8m33ea	70	28	specially	specially	RB
cord-021063-4y8m33ea	70	29	developed	develop	VBN
cord-021063-4y8m33ea	70	30	cell	cell	NN
cord-021063-4y8m33ea	70	31	lines	line	NNS
cord-021063-4y8m33ea	70	32	(	(	-LRB-
cord-021063-4y8m33ea	70	33	i.e.	i.e.	FW
cord-021063-4y8m33ea	70	34	,	,	,
cord-021063-4y8m33ea	70	35	packaging	packaging	NN
cord-021063-4y8m33ea	70	36	cell	cell	NN
cord-021063-4y8m33ea	70	37	lines	line	NNS
cord-021063-4y8m33ea	70	38	)	)	-RRB-
cord-021063-4y8m33ea	70	39	that	that	WDT
cord-021063-4y8m33ea	70	40	express	express	VBP
cord-021063-4y8m33ea	70	41	the	the	DT
cord-021063-4y8m33ea	70	42	removed	remove	VBN
cord-021063-4y8m33ea	70	43	proteins	protein	NNS
cord-021063-4y8m33ea	70	44	(	(	-LRB-
cord-021063-4y8m33ea	70	45	Kozarsky	Kozarsky	NNP
cord-021063-4y8m33ea	70	46	and	and	CC
cord-021063-4y8m33ea	70	47	Wilson	Wilson	NNP
cord-021063-4y8m33ea	70	48	,	,	,
cord-021063-4y8m33ea	70	49	1993	1993	CD
cord-021063-4y8m33ea	70	50	)	)	-RRB-
cord-021063-4y8m33ea	70	51	.	.	.
cord-021063-4y8m33ea	71	1	The	the	DT
cord-021063-4y8m33ea	71	2	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	71	3	gene	gene	NN
cord-021063-4y8m33ea	71	4	to	to	TO
cord-021063-4y8m33ea	71	5	be	be	VB
cord-021063-4y8m33ea	71	6	introduced	introduce	VBN
cord-021063-4y8m33ea	71	7	is	be	VBZ
cord-021063-4y8m33ea	71	8	added	add	VBN
cord-021063-4y8m33ea	71	9	to	to	IN
cord-021063-4y8m33ea	71	10	the	the	DT
cord-021063-4y8m33ea	71	11	adenoviral	adenoviral	JJ
cord-021063-4y8m33ea	71	12	genome	genome	NN
cord-021063-4y8m33ea	71	13	by	by	IN
cord-021063-4y8m33ea	71	14	homologous	homologous	JJ
cord-021063-4y8m33ea	71	15	recombination	recombination	NN
cord-021063-4y8m33ea	71	16	with	with	IN
cord-021063-4y8m33ea	71	17	a	a	DT
cord-021063-4y8m33ea	71	18	plasmid	plasmid	NN
cord-021063-4y8m33ea	71	19	containing	contain	VBG
cord-021063-4y8m33ea	71	20	the	the	DT
cord-021063-4y8m33ea	71	21	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	71	22	gene	gene	NN
cord-021063-4y8m33ea	71	23	flanked	flank	VBN
cord-021063-4y8m33ea	71	24	by	by	IN
cord-021063-4y8m33ea	71	25	adenoviral	adenoviral	NNP
cord-021063-4y8m33ea	71	26	DNA	DNA	NNP
cord-021063-4y8m33ea	71	27	.	.	.
cord-021063-4y8m33ea	72	1	Although	although	IN
cord-021063-4y8m33ea	72	2	possible	possible	JJ
cord-021063-4y8m33ea	72	3	target	target	NN
cord-021063-4y8m33ea	72	4	organs	organ	NNS
cord-021063-4y8m33ea	72	5	for	for	IN
cord-021063-4y8m33ea	72	6	adenoviral	adenoviral	JJ
cord-021063-4y8m33ea	72	7	gene	gene	NN
cord-021063-4y8m33ea	72	8	therapy	therapy	NN
cord-021063-4y8m33ea	72	9	include	include	VBP
cord-021063-4y8m33ea	72	10	liver	liver	NN
cord-021063-4y8m33ea	72	11	,	,	,
cord-021063-4y8m33ea	72	12	central	central	JJ
cord-021063-4y8m33ea	72	13	nervous	nervous	JJ
cord-021063-4y8m33ea	72	14	system	system	NN
cord-021063-4y8m33ea	72	15	,	,	,
cord-021063-4y8m33ea	72	16	vascular	vascular	JJ
cord-021063-4y8m33ea	72	17	endothelium	endothelium	NN
cord-021063-4y8m33ea	72	18	,	,	,
cord-021063-4y8m33ea	72	19	and	and	CC
cord-021063-4y8m33ea	72	20	muscle	muscle	NN
cord-021063-4y8m33ea	72	21	,	,	,
cord-021063-4y8m33ea	72	22	the	the	DT
cord-021063-4y8m33ea	72	23	primary	primary	JJ
cord-021063-4y8m33ea	72	24	target	target	NN
cord-021063-4y8m33ea	72	25	organ	organ	NN
cord-021063-4y8m33ea	72	26	is	be	VBZ
cord-021063-4y8m33ea	72	27	the	the	DT
cord-021063-4y8m33ea	72	28	lung	lung	NN
cord-021063-4y8m33ea	72	29	.	.	.
cord-021063-4y8m33ea	73	1	Clinical	clinical	JJ
cord-021063-4y8m33ea	73	2	trials	trial	NNS
cord-021063-4y8m33ea	73	3	of	of	IN
cord-021063-4y8m33ea	73	4	adenoviral	adenoviral	JJ
cord-021063-4y8m33ea	73	5	gene	gene	NN
cord-021063-4y8m33ea	73	6	therapy	therapy	NN
cord-021063-4y8m33ea	73	7	for	for	IN
cord-021063-4y8m33ea	73	8	treatment	treatment	NN
cord-021063-4y8m33ea	73	9	of	of	IN
cord-021063-4y8m33ea	73	10	~l	~l	NNP
cord-021063-4y8m33ea	73	11	-	-	HYPH
cord-021063-4y8m33ea	73	12	antitrypsin	antitrypsin	NN
cord-021063-4y8m33ea	73	13	deficiency	deficiency	NN
cord-021063-4y8m33ea	73	14	and	and	CC
cord-021063-4y8m33ea	73	15	cystic	cystic	JJ
cord-021063-4y8m33ea	73	16	fibrosis	fibrosis	NN
cord-021063-4y8m33ea	73	17	are	be	VBP
cord-021063-4y8m33ea	73	18	underway	underway	JJ
cord-021063-4y8m33ea	73	19	(	(	-LRB-
cord-021063-4y8m33ea	73	20	Crystal	Crystal	NNP
cord-021063-4y8m33ea	73	21	,	,	,
cord-021063-4y8m33ea	73	22	1992	1992	CD
cord-021063-4y8m33ea	73	23	)	)	-RRB-
cord-021063-4y8m33ea	73	24	.	.	.
cord-021063-4y8m33ea	74	1	Advantages	advantage	NNS
cord-021063-4y8m33ea	74	2	of	of	IN
cord-021063-4y8m33ea	74	3	adenovirus	adenovirus	NN
cord-021063-4y8m33ea	74	4	-	-	HYPH
cord-021063-4y8m33ea	74	5	based	base	VBN
cord-021063-4y8m33ea	74	6	vectors	vector	NNS
cord-021063-4y8m33ea	74	7	include	include	VBP
cord-021063-4y8m33ea	74	8	:	:	:
cord-021063-4y8m33ea	74	9	(	(	-LRB-
cord-021063-4y8m33ea	74	10	i	i	NN
cord-021063-4y8m33ea	74	11	)	)	-RRB-
cord-021063-4y8m33ea	75	1	the	the	DT
cord-021063-4y8m33ea	75	2	ability	ability	NN
cord-021063-4y8m33ea	75	3	to	to	TO
cord-021063-4y8m33ea	75	4	infect	infect	VB
cord-021063-4y8m33ea	75	5	large	large	JJ
cord-021063-4y8m33ea	75	6	numbers	number	NNS
cord-021063-4y8m33ea	75	7	of	of	IN
cord-021063-4y8m33ea	75	8	cells	cell	NNS
cord-021063-4y8m33ea	75	9	,	,	,
cord-021063-4y8m33ea	75	10	(	(	-LRB-
cord-021063-4y8m33ea	75	11	ii	ii	NN
cord-021063-4y8m33ea	75	12	)	)	-RRB-
cord-021063-4y8m33ea	75	13	relative	relative	JJ
cord-021063-4y8m33ea	75	14	ease	ease	NN
cord-021063-4y8m33ea	75	15	of	of	IN
cord-021063-4y8m33ea	75	16	constructing	construct	VBG
cord-021063-4y8m33ea	75	17	the	the	DT
cord-021063-4y8m33ea	75	18	vector	vector	NN
cord-021063-4y8m33ea	75	19	and	and	CC
cord-021063-4y8m33ea	75	20	obtaining	obtain	VBG
cord-021063-4y8m33ea	75	21	large	large	JJ
cord-021063-4y8m33ea	75	22	amounts	amount	NNS
cord-021063-4y8m33ea	75	23	of	of	IN
cord-021063-4y8m33ea	75	24	virus	virus	NN
cord-021063-4y8m33ea	75	25	,	,	,
cord-021063-4y8m33ea	75	26	and	and	CC
cord-021063-4y8m33ea	75	27	(	(	-LRB-
cord-021063-4y8m33ea	75	28	iii	iii	NN
cord-021063-4y8m33ea	75	29	)	)	-RRB-
cord-021063-4y8m33ea	75	30	absence	absence	NN
cord-021063-4y8m33ea	75	31	of	of	IN
cord-021063-4y8m33ea	75	32	genomic	genomic	JJ
cord-021063-4y8m33ea	75	33	integration	integration	NN
cord-021063-4y8m33ea	75	34	,	,	,
cord-021063-4y8m33ea	75	35	obviating	obviate	VBG
cord-021063-4y8m33ea	75	36	the	the	DT
cord-021063-4y8m33ea	75	37	problem	problem	NN
cord-021063-4y8m33ea	75	38	of	of	IN
cord-021063-4y8m33ea	75	39	insertional	insertional	JJ
cord-021063-4y8m33ea	75	40	mutagenesis	mutagenesis	NN
cord-021063-4y8m33ea	75	41	.	.	.
cord-021063-4y8m33ea	76	1	Some	some	DT
cord-021063-4y8m33ea	76	2	disadvantages	disadvantage	NNS
cord-021063-4y8m33ea	76	3	are	be	VBP
cord-021063-4y8m33ea	76	4	:	:	:
cord-021063-4y8m33ea	76	5	(	(	-LRB-
cord-021063-4y8m33ea	76	6	i	i	NN
cord-021063-4y8m33ea	76	7	)	)	-RRB-
cord-021063-4y8m33ea	77	1	the	the	DT
cord-021063-4y8m33ea	77	2	necessity	necessity	NN
cord-021063-4y8m33ea	77	3	to	to	TO
cord-021063-4y8m33ea	77	4	periodically	periodically	RB
cord-021063-4y8m33ea	77	5	reinfect	reinfect	VB
cord-021063-4y8m33ea	77	6	the	the	DT
cord-021063-4y8m33ea	77	7	patient	patient	NN
cord-021063-4y8m33ea	77	8	,	,	,
cord-021063-4y8m33ea	77	9	as	as	IN
cord-021063-4y8m33ea	77	10	the	the	DT
cord-021063-4y8m33ea	77	11	genome	genome	NN
cord-021063-4y8m33ea	77	12	is	be	VBZ
cord-021063-4y8m33ea	77	13	lost	lose	VBN
cord-021063-4y8m33ea	77	14	from	from	IN
cord-021063-4y8m33ea	77	15	dividing	divide	VBG
cord-021063-4y8m33ea	77	16	cells	cell	NNS
cord-021063-4y8m33ea	77	17	;	;	,
cord-021063-4y8m33ea	77	18	(	(	-LRB-
cord-021063-4y8m33ea	77	19	ii	ii	NN
cord-021063-4y8m33ea	77	20	)	)	-RRB-
cord-021063-4y8m33ea	78	1	the	the	DT
cord-021063-4y8m33ea	78	2	concomitant	concomitant	JJ
cord-021063-4y8m33ea	78	3	development	development	NN
cord-021063-4y8m33ea	78	4	of	of	IN
cord-021063-4y8m33ea	78	5	an	an	DT
cord-021063-4y8m33ea	78	6	allergic	allergic	JJ
cord-021063-4y8m33ea	78	7	response	response	NN
cord-021063-4y8m33ea	78	8	;	;	:
cord-021063-4y8m33ea	78	9	and	and	CC
cord-021063-4y8m33ea	78	10	(	(	-LRB-
cord-021063-4y8m33ea	78	11	iii	iii	NN
cord-021063-4y8m33ea	78	12	)	)	-RRB-
cord-021063-4y8m33ea	79	1	possible	possible	JJ
cord-021063-4y8m33ea	79	2	toxicity	toxicity	NN
cord-021063-4y8m33ea	79	3	of	of	IN
cord-021063-4y8m33ea	79	4	high	high	JJ
cord-021063-4y8m33ea	79	5	doses	dose	NNS
cord-021063-4y8m33ea	79	6	of	of	IN
cord-021063-4y8m33ea	79	7	virus	virus	NN
cord-021063-4y8m33ea	79	8	.	.	.
cord-021063-4y8m33ea	80	1	Adeno	Adeno	NNP
cord-021063-4y8m33ea	80	2	-	-	HYPH
cord-021063-4y8m33ea	80	3	associated	associate	VBN
cord-021063-4y8m33ea	80	4	virus	virus	NN
cord-021063-4y8m33ea	80	5	is	be	VBZ
cord-021063-4y8m33ea	80	6	a	a	DT
cord-021063-4y8m33ea	80	7	naturally	naturally	RB
cord-021063-4y8m33ea	80	8	occurring	occur	VBG
cord-021063-4y8m33ea	80	9	defective	defective	JJ
cord-021063-4y8m33ea	80	10	virus	virus	NN
cord-021063-4y8m33ea	80	11	that	that	WDT
cord-021063-4y8m33ea	80	12	infects	infect	VBZ
cord-021063-4y8m33ea	80	13	human	human	JJ
cord-021063-4y8m33ea	80	14	lungs	lung	NNS
cord-021063-4y8m33ea	80	15	.	.	.
cord-021063-4y8m33ea	81	1	Although	although	IN
cord-021063-4y8m33ea	81	2	it	-PRON-	PRP
cord-021063-4y8m33ea	81	3	is	be	VBZ
cord-021063-4y8m33ea	81	4	unable	unable	JJ
cord-021063-4y8m33ea	81	5	to	to	TO
cord-021063-4y8m33ea	81	6	go	go	VB
cord-021063-4y8m33ea	81	7	through	through	IN
cord-021063-4y8m33ea	81	8	lytic	lytic	JJ
cord-021063-4y8m33ea	81	9	infection	infection	NN
cord-021063-4y8m33ea	81	10	of	of	IN
cord-021063-4y8m33ea	81	11	cells	cell	NNS
cord-021063-4y8m33ea	81	12	independently	independently	RB
cord-021063-4y8m33ea	81	13	,	,	,
cord-021063-4y8m33ea	81	14	it	-PRON-	PRP
cord-021063-4y8m33ea	81	15	can	can	MD
cord-021063-4y8m33ea	81	16	do	do	VB
cord-021063-4y8m33ea	81	17	so	so	RB
cord-021063-4y8m33ea	81	18	in	in	IN
cord-021063-4y8m33ea	81	19	the	the	DT
cord-021063-4y8m33ea	81	20	presence	presence	NN
cord-021063-4y8m33ea	81	21	of	of	IN
cord-021063-4y8m33ea	81	22	a	a	DT
cord-021063-4y8m33ea	81	23	separate	separate	JJ
cord-021063-4y8m33ea	81	24	infection	infection	NN
cord-021063-4y8m33ea	81	25	by	by	IN
cord-021063-4y8m33ea	81	26	adenovirus	adenovirus	NN
cord-021063-4y8m33ea	81	27	or	or	CC
cord-021063-4y8m33ea	81	28	herpes	herpes	NN
cord-021063-4y8m33ea	81	29	simplex	simplex	NN
cord-021063-4y8m33ea	81	30	virus	virus	NN
cord-021063-4y8m33ea	81	31	.	.	.
cord-021063-4y8m33ea	82	1	In	in	IN
cord-021063-4y8m33ea	82	2	the	the	DT
cord-021063-4y8m33ea	82	3	absence	absence	NN
cord-021063-4y8m33ea	82	4	of	of	IN
cord-021063-4y8m33ea	82	5	a	a	DT
cord-021063-4y8m33ea	82	6	helper	helper	NN
cord-021063-4y8m33ea	82	7	infection	infection	NN
cord-021063-4y8m33ea	82	8	by	by	IN
cord-021063-4y8m33ea	82	9	one	one	CD
cord-021063-4y8m33ea	82	10	of	of	IN
cord-021063-4y8m33ea	82	11	these	these	DT
cord-021063-4y8m33ea	82	12	viruses	virus	NNS
cord-021063-4y8m33ea	82	13	,	,	,
cord-021063-4y8m33ea	82	14	the	the	DT
cord-021063-4y8m33ea	82	15	adeno	adeno	NN
cord-021063-4y8m33ea	82	16	-	-	HYPH
cord-021063-4y8m33ea	82	17	associated	associate	VBN
cord-021063-4y8m33ea	82	18	virus	virus	NN
cord-021063-4y8m33ea	82	19	establishes	establish	VBZ
cord-021063-4y8m33ea	82	20	a	a	DT
cord-021063-4y8m33ea	82	21	latent	latent	JJ
cord-021063-4y8m33ea	82	22	infection	infection	NN
cord-021063-4y8m33ea	82	23	of	of	IN
cord-021063-4y8m33ea	82	24	the	the	DT
cord-021063-4y8m33ea	82	25	host	host	NN
cord-021063-4y8m33ea	82	26	cell	cell	NN
cord-021063-4y8m33ea	82	27	by	by	IN
cord-021063-4y8m33ea	82	28	integrating	integrate	VBG
cord-021063-4y8m33ea	82	29	itself	-PRON-	PRP
cord-021063-4y8m33ea	82	30	into	into	IN
cord-021063-4y8m33ea	82	31	the	the	DT
cord-021063-4y8m33ea	82	32	host	host	NN
cord-021063-4y8m33ea	82	33	genome	genome	NN
cord-021063-4y8m33ea	82	34	.	.	.
cord-021063-4y8m33ea	83	1	About	about	RB
cord-021063-4y8m33ea	83	2	70	70	CD
cord-021063-4y8m33ea	83	3	%	%	NN
cord-021063-4y8m33ea	83	4	of	of	IN
cord-021063-4y8m33ea	83	5	the	the	DT
cord-021063-4y8m33ea	83	6	integrations	integration	NNS
cord-021063-4y8m33ea	83	7	of	of	IN
cord-021063-4y8m33ea	83	8	the	the	DT
cord-021063-4y8m33ea	83	9	wild	wild	JJ
cord-021063-4y8m33ea	83	10	-	-	HYPH
cord-021063-4y8m33ea	83	11	type	type	NN
cord-021063-4y8m33ea	83	12	virus	virus	NN
cord-021063-4y8m33ea	83	13	occur	occur	VBP
cord-021063-4y8m33ea	83	14	at	at	IN
cord-021063-4y8m33ea	83	15	a	a	DT
cord-021063-4y8m33ea	83	16	single	single	JJ
cord-021063-4y8m33ea	83	17	site	site	NN
cord-021063-4y8m33ea	83	18	on	on	IN
cord-021063-4y8m33ea	83	19	chromosome	chromosome	NN
cord-021063-4y8m33ea	83	20	19	19	CD
cord-021063-4y8m33ea	84	1	(	(	-LRB-
cord-021063-4y8m33ea	84	2	Samulski	Samulski	NNP
cord-021063-4y8m33ea	84	3	et	et	NNP
cord-021063-4y8m33ea	84	4	al	al	NNP
cord-021063-4y8m33ea	84	5	.	.	NNP
cord-021063-4y8m33ea	84	6	,	,	,
cord-021063-4y8m33ea	84	7	1991	1991	CD
cord-021063-4y8m33ea	84	8	)	)	-RRB-
cord-021063-4y8m33ea	84	9	.	.	.
cord-021063-4y8m33ea	85	1	Because	because	IN
cord-021063-4y8m33ea	85	2	these	these	DT
cord-021063-4y8m33ea	85	3	integrations	integration	NNS
cord-021063-4y8m33ea	85	4	do	do	VBP
cord-021063-4y8m33ea	85	5	not	not	RB
cord-021063-4y8m33ea	85	6	seem	seem	VB
cord-021063-4y8m33ea	85	7	to	to	TO
cord-021063-4y8m33ea	85	8	be	be	VB
cord-021063-4y8m33ea	85	9	associated	associate	VBN
cord-021063-4y8m33ea	85	10	with	with	IN
cord-021063-4y8m33ea	85	11	any	any	DT
cord-021063-4y8m33ea	85	12	disease	disease	NN
cord-021063-4y8m33ea	85	13	,	,	,
cord-021063-4y8m33ea	85	14	this	this	DT
cord-021063-4y8m33ea	85	15	virus	virus	NN
cord-021063-4y8m33ea	85	16	may	may	MD
cord-021063-4y8m33ea	85	17	eventually	eventually	RB
cord-021063-4y8m33ea	85	18	be	be	VB
cord-021063-4y8m33ea	85	19	used	use	VBN
cord-021063-4y8m33ea	85	20	to	to	TO
cord-021063-4y8m33ea	85	21	add	add	VB
cord-021063-4y8m33ea	85	22	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	85	23	genes	gene	NNS
cord-021063-4y8m33ea	85	24	to	to	IN
cord-021063-4y8m33ea	85	25	the	the	DT
cord-021063-4y8m33ea	85	26	human	human	JJ
cord-021063-4y8m33ea	85	27	genome	genome	NN
cord-021063-4y8m33ea	85	28	with	with	IN
cord-021063-4y8m33ea	85	29	a	a	DT
cord-021063-4y8m33ea	85	30	lower	low	JJR
cord-021063-4y8m33ea	85	31	risk	risk	NN
cord-021063-4y8m33ea	85	32	of	of	IN
cord-021063-4y8m33ea	85	33	insertional	insertional	JJ
cord-021063-4y8m33ea	85	34	mutagenesis	mutagenesis	NN
cord-021063-4y8m33ea	85	35	.	.	.
cord-021063-4y8m33ea	86	1	To	to	IN
cord-021063-4y8m33ea	86	2	date	date	NN
cord-021063-4y8m33ea	86	3	,	,	,
cord-021063-4y8m33ea	86	4	however	however	RB
cord-021063-4y8m33ea	86	5	,	,	,
cord-021063-4y8m33ea	86	6	the	the	DT
cord-021063-4y8m33ea	86	7	targeted	targeted	JJ
cord-021063-4y8m33ea	86	8	nature	nature	NN
cord-021063-4y8m33ea	86	9	of	of	IN
cord-021063-4y8m33ea	86	10	the	the	DT
cord-021063-4y8m33ea	86	11	insertional	insertional	JJ
cord-021063-4y8m33ea	86	12	events	event	NNS
cord-021063-4y8m33ea	86	13	is	be	VBZ
cord-021063-4y8m33ea	86	14	not	not	RB
cord-021063-4y8m33ea	86	15	retained	retain	VBN
cord-021063-4y8m33ea	86	16	when	when	WRB
cord-021063-4y8m33ea	86	17	the	the	DT
cord-021063-4y8m33ea	86	18	native	native	JJ
cord-021063-4y8m33ea	86	19	viral	viral	JJ
cord-021063-4y8m33ea	86	20	genome	genome	NN
cord-021063-4y8m33ea	86	21	is	be	VBZ
cord-021063-4y8m33ea	86	22	replaced	replace	VBN
cord-021063-4y8m33ea	86	23	by	by	IN
cord-021063-4y8m33ea	86	24	a	a	DT
cord-021063-4y8m33ea	86	25	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	86	26	or	or	CC
cord-021063-4y8m33ea	86	27	marker	marker	NN
cord-021063-4y8m33ea	86	28	gene	gene	NN
cord-021063-4y8m33ea	86	29	.	.	.
cord-021063-4y8m33ea	87	1	In	in	IN
cord-021063-4y8m33ea	87	2	addition	addition	NN
cord-021063-4y8m33ea	87	3	,	,	,
cord-021063-4y8m33ea	87	4	packaging	packaging	NN
cord-021063-4y8m33ea	87	5	constraints	constraint	NNS
cord-021063-4y8m33ea	87	6	limit	limit	VBP
cord-021063-4y8m33ea	87	7	the	the	DT
cord-021063-4y8m33ea	87	8	size	size	NN
cord-021063-4y8m33ea	87	9	of	of	IN
cord-021063-4y8m33ea	87	10	any	any	DT
cord-021063-4y8m33ea	87	11	insert	insert	NN
cord-021063-4y8m33ea	87	12	to	to	IN
cord-021063-4y8m33ea	87	13	4.5	4.5	CD
cord-021063-4y8m33ea	87	14	-	-	SYM
cord-021063-4y8m33ea	87	15	5	5	CD
cord-021063-4y8m33ea	87	16	kb	kb	NNP
cord-021063-4y8m33ea	87	17	of	of	IN
cord-021063-4y8m33ea	87	18	DNA	DNA	NNP
cord-021063-4y8m33ea	87	19	.	.	.
cord-021063-4y8m33ea	88	1	The	the	DT
cord-021063-4y8m33ea	88	2	genomes	genome	NNS
cord-021063-4y8m33ea	88	3	of	of	IN
cord-021063-4y8m33ea	88	4	herpesviruses	herpesviruse	NNS
cord-021063-4y8m33ea	88	5	are	be	VBP
cord-021063-4y8m33ea	88	6	very	very	RB
cord-021063-4y8m33ea	88	7	large	large	JJ
cord-021063-4y8m33ea	88	8	and	and	CC
cord-021063-4y8m33ea	88	9	complex	complex	JJ
cord-021063-4y8m33ea	88	10	.	.	.
cord-021063-4y8m33ea	89	1	This	this	DT
cord-021063-4y8m33ea	89	2	fact	fact	NN
cord-021063-4y8m33ea	89	3	alone	alone	RB
cord-021063-4y8m33ea	89	4	might	may	MD
cord-021063-4y8m33ea	89	5	argue	argue	VB
cord-021063-4y8m33ea	89	6	against	against	IN
cord-021063-4y8m33ea	89	7	the	the	DT
cord-021063-4y8m33ea	89	8	use	use	NN
cord-021063-4y8m33ea	89	9	of	of	IN
cord-021063-4y8m33ea	89	10	herpes	herpes	NNP
cord-021063-4y8m33ea	89	11	simplex	simplex	NNP
cord-021063-4y8m33ea	89	12	virus	virus	NN
cord-021063-4y8m33ea	90	1	I	-PRON-	PRP
cord-021063-4y8m33ea	90	2	(	(	-LRB-
cord-021063-4y8m33ea	90	3	HSV	HSV	NNP
cord-021063-4y8m33ea	90	4	-	-	HYPH
cord-021063-4y8m33ea	90	5	I	I	NNP
cord-021063-4y8m33ea	90	6	)	)	-RRB-
cord-021063-4y8m33ea	90	7	as	as	IN
cord-021063-4y8m33ea	90	8	a	a	DT
cord-021063-4y8m33ea	90	9	gene	gene	NN
cord-021063-4y8m33ea	90	10	therapy	therapy	NN
cord-021063-4y8m33ea	90	11	vector	vector	NN
cord-021063-4y8m33ea	90	12	,	,	,
cord-021063-4y8m33ea	90	13	since	since	IN
cord-021063-4y8m33ea	90	14	a	a	DT
cord-021063-4y8m33ea	90	15	complex	complex	JJ
cord-021063-4y8m33ea	90	16	genome	genome	NN
cord-021063-4y8m33ea	90	17	will	will	MD
cord-021063-4y8m33ea	90	18	be	be	VB
cord-021063-4y8m33ea	90	19	more	more	RBR
cord-021063-4y8m33ea	90	20	difficult	difficult	JJ
cord-021063-4y8m33ea	90	21	to	to	TO
cord-021063-4y8m33ea	90	22	effectively	effectively	RB
cord-021063-4y8m33ea	90	23	"	"	``
cord-021063-4y8m33ea	90	24	tame	tame	JJ
cord-021063-4y8m33ea	90	25	.	.	.
cord-021063-4y8m33ea	90	26	"	"	''
cord-021063-4y8m33ea	91	1	However	however	RB
cord-021063-4y8m33ea	91	2	,	,	,
cord-021063-4y8m33ea	91	3	HSV	HSV	NNP
cord-021063-4y8m33ea	91	4	-	-	HYPH
cord-021063-4y8m33ea	91	5	I	-PRON-	PRP
cord-021063-4y8m33ea	91	6	has	have	VBZ
cord-021063-4y8m33ea	91	7	an	an	DT
cord-021063-4y8m33ea	91	8	advantage	advantage	NN
cord-021063-4y8m33ea	91	9	that	that	WDT
cord-021063-4y8m33ea	91	10	compensates	compensate	VBZ
cord-021063-4y8m33ea	91	11	for	for	IN
cord-021063-4y8m33ea	91	12	this	this	DT
cord-021063-4y8m33ea	91	13	problem	problem	NN
cord-021063-4y8m33ea	91	14	,	,	,
cord-021063-4y8m33ea	91	15	in	in	IN
cord-021063-4y8m33ea	91	16	that	that	IN
cord-021063-4y8m33ea	91	17	the	the	DT
cord-021063-4y8m33ea	91	18	virus	virus	NN
cord-021063-4y8m33ea	91	19	can	can	MD
cord-021063-4y8m33ea	91	20	exist	exist	VB
cord-021063-4y8m33ea	91	21	and	and	CC
cord-021063-4y8m33ea	91	22	mediate	mediate	VB
cord-021063-4y8m33ea	91	23	persistent	persistent	JJ
cord-021063-4y8m33ea	91	24	expression	expression	NN
cord-021063-4y8m33ea	91	25	of	of	IN
cord-021063-4y8m33ea	91	26	its	-PRON-	PRP$
cord-021063-4y8m33ea	91	27	genes	gene	NNS
cord-021063-4y8m33ea	91	28	in	in	IN
cord-021063-4y8m33ea	91	29	neurons	neuron	NNS
cord-021063-4y8m33ea	91	30	and	and	CC
cord-021063-4y8m33ea	91	31	other	other	JJ
cord-021063-4y8m33ea	91	32	postmitotic	postmitotic	JJ
cord-021063-4y8m33ea	91	33	cells	cell	NNS
cord-021063-4y8m33ea	91	34	(	(	-LRB-
cord-021063-4y8m33ea	91	35	Geller	Geller	NNP
cord-021063-4y8m33ea	91	36	,	,	,
cord-021063-4y8m33ea	91	37	1993	1993	CD
cord-021063-4y8m33ea	91	38	)	)	-RRB-
cord-021063-4y8m33ea	91	39	.	.	.
cord-021063-4y8m33ea	92	1	In	in	IN
cord-021063-4y8m33ea	92	2	the	the	DT
cord-021063-4y8m33ea	92	3	normal	normal	JJ
cord-021063-4y8m33ea	92	4	course	course	NN
cord-021063-4y8m33ea	92	5	of	of	IN
cord-021063-4y8m33ea	92	6	an	an	DT
cord-021063-4y8m33ea	92	7	infection	infection	NN
cord-021063-4y8m33ea	92	8	,	,	,
cord-021063-4y8m33ea	92	9	HSV	HSV	NNP
cord-021063-4y8m33ea	92	10	-	-	:
cord-021063-4y8m33ea	92	11	I	-PRON-	PRP
cord-021063-4y8m33ea	92	12	will	will	MD
cord-021063-4y8m33ea	92	13	enter	enter	VB
cord-021063-4y8m33ea	92	14	neuronal	neuronal	JJ
cord-021063-4y8m33ea	92	15	cells	cell	NNS
cord-021063-4y8m33ea	92	16	and	and	CC
cord-021063-4y8m33ea	92	17	remain	remain	VBP
cord-021063-4y8m33ea	92	18	latent	latent	JJ
cord-021063-4y8m33ea	92	19	,	,	,
cord-021063-4y8m33ea	92	20	sometimes	sometimes	RB
cord-021063-4y8m33ea	92	21	for	for	IN
cord-021063-4y8m33ea	92	22	years	year	NNS
cord-021063-4y8m33ea	92	23	,	,	,
cord-021063-4y8m33ea	92	24	before	before	IN
cord-021063-4y8m33ea	92	25	reactivating	reactivate	VBG
cord-021063-4y8m33ea	92	26	and	and	CC
cord-021063-4y8m33ea	92	27	starting	start	VBG
cord-021063-4y8m33ea	92	28	a	a	DT
cord-021063-4y8m33ea	92	29	lytic	lytic	JJ
cord-021063-4y8m33ea	92	30	cycle	cycle	NN
cord-021063-4y8m33ea	92	31	that	that	WDT
cord-021063-4y8m33ea	92	32	results	result	VBZ
cord-021063-4y8m33ea	92	33	in	in	IN
cord-021063-4y8m33ea	92	34	a	a	DT
cord-021063-4y8m33ea	92	35	small	small	JJ
cord-021063-4y8m33ea	92	36	patch	patch	NN
cord-021063-4y8m33ea	92	37	of	of	IN
cord-021063-4y8m33ea	92	38	endothelial	endothelial	JJ
cord-021063-4y8m33ea	92	39	cell	cell	NN
cord-021063-4y8m33ea	92	40	death	death	NN
cord-021063-4y8m33ea	92	41	(	(	-LRB-
cord-021063-4y8m33ea	92	42	e.g.	e.g.	RB
cord-021063-4y8m33ea	92	43	,	,	,
cord-021063-4y8m33ea	92	44	a	a	DT
cord-021063-4y8m33ea	92	45	cold	cold	JJ
cord-021063-4y8m33ea	92	46	sore	sore	NN
cord-021063-4y8m33ea	92	47	)	)	-RRB-
cord-021063-4y8m33ea	92	48	.	.	.
cord-021063-4y8m33ea	93	1	During	during	IN
cord-021063-4y8m33ea	93	2	latency	latency	NNP
cord-021063-4y8m33ea	93	3	,	,	,
cord-021063-4y8m33ea	93	4	the	the	DT
cord-021063-4y8m33ea	93	5	host	host	NN
cord-021063-4y8m33ea	93	6	cell	cell	NN
cord-021063-4y8m33ea	93	7	transcribes	transcribe	VBZ
cord-021063-4y8m33ea	93	8	some	some	DT
cord-021063-4y8m33ea	93	9	viral	viral	JJ
cord-021063-4y8m33ea	93	10	genes	gene	NNS
cord-021063-4y8m33ea	93	11	,	,	,
cord-021063-4y8m33ea	93	12	producing	produce	VBG
cord-021063-4y8m33ea	93	13	what	what	WP
cord-021063-4y8m33ea	93	14	are	be	VBP
cord-021063-4y8m33ea	93	15	termed	term	VBN
cord-021063-4y8m33ea	93	16	latency	latency	NN
cord-021063-4y8m33ea	93	17	associated	associate	VBN
cord-021063-4y8m33ea	93	18	transcripts	transcript	NNS
cord-021063-4y8m33ea	93	19	(	(	-LRB-
cord-021063-4y8m33ea	93	20	LATs	LATs	NNP
cord-021063-4y8m33ea	93	21	)	)	-RRB-
cord-021063-4y8m33ea	93	22	.	.	.
cord-021063-4y8m33ea	94	1	HSV	HSV	NNP
cord-021063-4y8m33ea	94	2	-	-	HYPH
cord-021063-4y8m33ea	94	3	I	-PRON-	PRP
cord-021063-4y8m33ea	94	4	deletion	deletion	NN
cord-021063-4y8m33ea	94	5	mutants	mutant	NNS
cord-021063-4y8m33ea	94	6	have	have	VBP
cord-021063-4y8m33ea	94	7	been	be	VBN
cord-021063-4y8m33ea	94	8	isolated	isolate	VBN
cord-021063-4y8m33ea	94	9	that	that	WDT
cord-021063-4y8m33ea	94	10	are	be	VBP
cord-021063-4y8m33ea	94	11	incapable	incapable	JJ
cord-021063-4y8m33ea	94	12	of	of	IN
cord-021063-4y8m33ea	94	13	reactivation	reactivation	NN
cord-021063-4y8m33ea	94	14	,	,	,
cord-021063-4y8m33ea	94	15	and	and	CC
cord-021063-4y8m33ea	94	16	that	that	IN
cord-021063-4y8m33ea	94	17	once	once	RB
cord-021063-4y8m33ea	94	18	inserted	insert	VBD
cord-021063-4y8m33ea	94	19	into	into	IN
cord-021063-4y8m33ea	94	20	a	a	DT
cord-021063-4y8m33ea	94	21	neuron	neuron	NN
cord-021063-4y8m33ea	94	22	will	will	MD
cord-021063-4y8m33ea	94	23	remain	remain	VB
cord-021063-4y8m33ea	94	24	latent	latent	JJ
cord-021063-4y8m33ea	94	25	indefinitely	indefinitely	RB
cord-021063-4y8m33ea	94	26	.	.	.
cord-021063-4y8m33ea	95	1	If	if	IN
cord-021063-4y8m33ea	95	2	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	95	3	genes	gene	NNS
cord-021063-4y8m33ea	95	4	added	add	VBD
cord-021063-4y8m33ea	95	5	to	to	IN
cord-021063-4y8m33ea	95	6	these	these	DT
cord-021063-4y8m33ea	95	7	vectors	vector	NNS
cord-021063-4y8m33ea	95	8	have	have	VBP
cord-021063-4y8m33ea	95	9	LAT	lat	NN
cord-021063-4y8m33ea	95	10	promoter	promoter	NN
cord-021063-4y8m33ea	95	11	elements	element	NNS
cord-021063-4y8m33ea	95	12	,	,	,
cord-021063-4y8m33ea	95	13	they	-PRON-	PRP
cord-021063-4y8m33ea	95	14	will	will	MD
cord-021063-4y8m33ea	95	15	be	be	VB
cord-021063-4y8m33ea	95	16	expressed	express	VBN
cord-021063-4y8m33ea	95	17	even	even	RB
cord-021063-4y8m33ea	95	18	though	though	IN
cord-021063-4y8m33ea	95	19	the	the	DT
cord-021063-4y8m33ea	95	20	virus	virus	NN
cord-021063-4y8m33ea	95	21	itself	-PRON-	PRP
cord-021063-4y8m33ea	95	22	remains	remain	VBZ
cord-021063-4y8m33ea	95	23	latent	latent	JJ
cord-021063-4y8m33ea	95	24	.	.	.
cord-021063-4y8m33ea	96	1	These	these	DT
cord-021063-4y8m33ea	96	2	vectors	vector	NNS
cord-021063-4y8m33ea	96	3	are	be	VBP
cord-021063-4y8m33ea	96	4	being	be	VBG
cord-021063-4y8m33ea	96	5	developed	develop	VBN
cord-021063-4y8m33ea	96	6	for	for	IN
cord-021063-4y8m33ea	96	7	the	the	DT
cord-021063-4y8m33ea	96	8	introduction	introduction	NN
cord-021063-4y8m33ea	96	9	of	of	IN
cord-021063-4y8m33ea	96	10	genes	gene	NNS
cord-021063-4y8m33ea	96	11	into	into	IN
cord-021063-4y8m33ea	96	12	cells	cell	NNS
cord-021063-4y8m33ea	96	13	of	of	IN
cord-021063-4y8m33ea	96	14	the	the	DT
cord-021063-4y8m33ea	96	15	central	central	JJ
cord-021063-4y8m33ea	96	16	nervous	nervous	JJ
cord-021063-4y8m33ea	96	17	system	system	NN
cord-021063-4y8m33ea	96	18	(	(	-LRB-
cord-021063-4y8m33ea	96	19	Geller	Geller	NNP
cord-021063-4y8m33ea	96	20	,	,	,
cord-021063-4y8m33ea	96	21	1993	1993	CD
cord-021063-4y8m33ea	96	22	)	)	-RRB-
cord-021063-4y8m33ea	96	23	.	.	.
cord-021063-4y8m33ea	97	1	In	in	IN
cord-021063-4y8m33ea	97	2	summary	summary	NN
cord-021063-4y8m33ea	97	3	,	,	,
cord-021063-4y8m33ea	97	4	all	all	DT
cord-021063-4y8m33ea	97	5	viral	viral	JJ
cord-021063-4y8m33ea	97	6	methods	method	NNS
cord-021063-4y8m33ea	97	7	of	of	IN
cord-021063-4y8m33ea	97	8	gene	gene	NN
cord-021063-4y8m33ea	97	9	therapy	therapy	NN
cord-021063-4y8m33ea	97	10	share	share	NN
cord-021063-4y8m33ea	97	11	several	several	JJ
cord-021063-4y8m33ea	97	12	strengths	strength	NNS
cord-021063-4y8m33ea	97	13	,	,	,
cord-021063-4y8m33ea	97	14	as	as	RB
cord-021063-4y8m33ea	97	15	well	well	RB
cord-021063-4y8m33ea	97	16	as	as	IN
cord-021063-4y8m33ea	97	17	numerous	numerous	JJ
cord-021063-4y8m33ea	97	18	weaknesses	weakness	NNS
cord-021063-4y8m33ea	97	19	.	.	.
cord-021063-4y8m33ea	98	1	It	-PRON-	PRP
cord-021063-4y8m33ea	98	2	is	be	VBZ
cord-021063-4y8m33ea	98	3	relatively	relatively	RB
cord-021063-4y8m33ea	98	4	easy	easy	JJ
cord-021063-4y8m33ea	98	5	to	to	TO
cord-021063-4y8m33ea	98	6	produce	produce	VB
cord-021063-4y8m33ea	98	7	the	the	DT
cord-021063-4y8m33ea	98	8	large	large	JJ
cord-021063-4y8m33ea	98	9	amounts	amount	NNS
cord-021063-4y8m33ea	98	10	of	of	IN
cord-021063-4y8m33ea	98	11	virus	virus	NN
cord-021063-4y8m33ea	98	12	necessary	necessary	JJ
cord-021063-4y8m33ea	98	13	to	to	TO
cord-021063-4y8m33ea	98	14	treat	treat	VB
cord-021063-4y8m33ea	98	15	patients	patient	NNS
cord-021063-4y8m33ea	98	16	.	.	.
cord-021063-4y8m33ea	99	1	Viruses	virus	NNS
cord-021063-4y8m33ea	99	2	efficiently	efficiently	RB
cord-021063-4y8m33ea	99	3	infect	infect	VBP
cord-021063-4y8m33ea	99	4	a	a	DT
cord-021063-4y8m33ea	99	5	large	large	JJ
cord-021063-4y8m33ea	99	6	fraction	fraction	NN
cord-021063-4y8m33ea	99	7	of	of	IN
cord-021063-4y8m33ea	99	8	the	the	DT
cord-021063-4y8m33ea	99	9	cells	cell	NNS
cord-021063-4y8m33ea	99	10	with	with	IN
cord-021063-4y8m33ea	99	11	which	which	WDT
cord-021063-4y8m33ea	99	12	they	-PRON-	PRP
cord-021063-4y8m33ea	99	13	come	come	VBP
cord-021063-4y8m33ea	99	14	in	in	IN
cord-021063-4y8m33ea	99	15	contact	contact	NN
cord-021063-4y8m33ea	99	16	.	.	.
cord-021063-4y8m33ea	100	1	Although	although	IN
cord-021063-4y8m33ea	100	2	this	this	DT
cord-021063-4y8m33ea	100	3	may	may	MD
cord-021063-4y8m33ea	100	4	be	be	VB
cord-021063-4y8m33ea	100	5	a	a	DT
cord-021063-4y8m33ea	100	6	drawback	drawback	NN
cord-021063-4y8m33ea	100	7	in	in	IN
cord-021063-4y8m33ea	100	8	some	some	DT
cord-021063-4y8m33ea	100	9	cases	case	NNS
cord-021063-4y8m33ea	100	10	where	where	WRB
cord-021063-4y8m33ea	100	11	expression	expression	NN
cord-021063-4y8m33ea	100	12	of	of	IN
cord-021063-4y8m33ea	100	13	the	the	DT
cord-021063-4y8m33ea	100	14	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	100	15	gene	gene	NN
cord-021063-4y8m33ea	100	16	must	must	MD
cord-021063-4y8m33ea	100	17	be	be	VB
cord-021063-4y8m33ea	100	18	restricted	restrict	VBN
cord-021063-4y8m33ea	100	19	to	to	IN
cord-021063-4y8m33ea	100	20	specific	specific	JJ
cord-021063-4y8m33ea	100	21	cells	cell	NNS
cord-021063-4y8m33ea	100	22	,	,	,
cord-021063-4y8m33ea	100	23	it	-PRON-	PRP
cord-021063-4y8m33ea	100	24	may	may	MD
cord-021063-4y8m33ea	100	25	be	be	VB
cord-021063-4y8m33ea	100	26	possible	possible	JJ
cord-021063-4y8m33ea	100	27	to	to	TO
cord-021063-4y8m33ea	100	28	limit	limit	VB
cord-021063-4y8m33ea	100	29	unwanted	unwanted	JJ
cord-021063-4y8m33ea	100	30	expression	expression	NN
cord-021063-4y8m33ea	100	31	of	of	IN
cord-021063-4y8m33ea	100	32	the	the	DT
cord-021063-4y8m33ea	100	33	inserted	insert	VBN
cord-021063-4y8m33ea	100	34	gene	gene	NN
cord-021063-4y8m33ea	100	35	by	by	IN
cord-021063-4y8m33ea	100	36	the	the	DT
cord-021063-4y8m33ea	100	37	use	use	NN
cord-021063-4y8m33ea	100	38	of	of	IN
cord-021063-4y8m33ea	100	39	tissue	tissue	NN
cord-021063-4y8m33ea	100	40	-	-	HYPH
cord-021063-4y8m33ea	100	41	specific	specific	JJ
cord-021063-4y8m33ea	100	42	promoters	promoter	NNS
cord-021063-4y8m33ea	100	43	.	.	.
cord-021063-4y8m33ea	101	1	All	all	DT
cord-021063-4y8m33ea	101	2	viral	viral	JJ
cord-021063-4y8m33ea	101	3	vectors	vector	NNS
cord-021063-4y8m33ea	101	4	for	for	IN
cord-021063-4y8m33ea	101	5	human	human	JJ
cord-021063-4y8m33ea	101	6	gene	gene	NN
cord-021063-4y8m33ea	101	7	therapy	therapy	NN
cord-021063-4y8m33ea	101	8	are	be	VBP
cord-021063-4y8m33ea	101	9	derived	derive	VBN
cord-021063-4y8m33ea	101	10	from	from	IN
cord-021063-4y8m33ea	101	11	organisms	organism	NNS
cord-021063-4y8m33ea	101	12	that	that	WDT
cord-021063-4y8m33ea	101	13	cause	cause	VBP
cord-021063-4y8m33ea	101	14	human	human	JJ
cord-021063-4y8m33ea	101	15	disease	disease	NN
cord-021063-4y8m33ea	101	16	.	.	.
cord-021063-4y8m33ea	102	1	The	the	DT
cord-021063-4y8m33ea	102	2	possibility	possibility	NN
cord-021063-4y8m33ea	102	3	of	of	IN
cord-021063-4y8m33ea	102	4	reactivation	reactivation	NN
cord-021063-4y8m33ea	102	5	of	of	IN
cord-021063-4y8m33ea	102	6	the	the	DT
cord-021063-4y8m33ea	102	7	vectors	vector	NNS
cord-021063-4y8m33ea	102	8	'	'	POS
cord-021063-4y8m33ea	102	9	infectivity	infectivity	NN
cord-021063-4y8m33ea	102	10	or	or	CC
cord-021063-4y8m33ea	102	11	pathogenicity	pathogenicity	NN
cord-021063-4y8m33ea	102	12	is	be	VBZ
cord-021063-4y8m33ea	102	13	hard	hard	JJ
cord-021063-4y8m33ea	102	14	to	to	TO
cord-021063-4y8m33ea	102	15	dismiss	dismiss	VB
cord-021063-4y8m33ea	102	16	.	.	.
cord-021063-4y8m33ea	103	1	In	in	IN
cord-021063-4y8m33ea	103	2	the	the	DT
cord-021063-4y8m33ea	103	3	end	end	NN
cord-021063-4y8m33ea	103	4	,	,	,
cord-021063-4y8m33ea	103	5	it	-PRON-	PRP
cord-021063-4y8m33ea	103	6	may	may	MD
cord-021063-4y8m33ea	103	7	prove	prove	VB
cord-021063-4y8m33ea	103	8	easier	easy	JJR
cord-021063-4y8m33ea	103	9	and	and	CC
cord-021063-4y8m33ea	103	10	safer	safe	JJR
cord-021063-4y8m33ea	103	11	to	to	TO
cord-021063-4y8m33ea	103	12	construct	construct	VB
cord-021063-4y8m33ea	103	13	a	a	DT
cord-021063-4y8m33ea	103	14	gene	gene	NN
cord-021063-4y8m33ea	103	15	therapy	therapy	NN
cord-021063-4y8m33ea	103	16	vector	vector	NN
cord-021063-4y8m33ea	103	17	having	have	VBG
cord-021063-4y8m33ea	103	18	the	the	DT
cord-021063-4y8m33ea	103	19	elements	element	NNS
cord-021063-4y8m33ea	103	20	of	of	IN
cord-021063-4y8m33ea	103	21	a	a	DT
cord-021063-4y8m33ea	103	22	successful	successful	JJ
cord-021063-4y8m33ea	103	23	virus	virus	NN
cord-021063-4y8m33ea	103	24	,	,	,
cord-021063-4y8m33ea	103	25	but	but	CC
cord-021063-4y8m33ea	103	26	derived	derive	VBN
cord-021063-4y8m33ea	103	27	from	from	IN
cord-021063-4y8m33ea	103	28	completely	completely	RB
cord-021063-4y8m33ea	103	29	synthetic	synthetic	JJ
cord-021063-4y8m33ea	103	30	components	component	NNS
cord-021063-4y8m33ea	103	31	.	.	.
cord-021063-4y8m33ea	104	1	One	one	CD
cord-021063-4y8m33ea	104	2	such	such	JJ
cord-021063-4y8m33ea	104	3	strategy	strategy	NN
cord-021063-4y8m33ea	104	4	involves	involve	VBZ
cord-021063-4y8m33ea	104	5	the	the	DT
cord-021063-4y8m33ea	104	6	use	use	NN
cord-021063-4y8m33ea	104	7	of	of	IN
cord-021063-4y8m33ea	104	8	liposomes	liposome	NNS
cord-021063-4y8m33ea	104	9	.	.	.
cord-021063-4y8m33ea	105	1	Liposomes	liposome	NNS
cord-021063-4y8m33ea	105	2	are	be	VBP
cord-021063-4y8m33ea	105	3	small	small	JJ
cord-021063-4y8m33ea	105	4	vesicles	vesicle	NNS
cord-021063-4y8m33ea	105	5	usually	usually	RB
cord-021063-4y8m33ea	105	6	made	make	VBN
cord-021063-4y8m33ea	105	7	from	from	IN
cord-021063-4y8m33ea	105	8	pure	pure	JJ
cord-021063-4y8m33ea	105	9	preparations	preparation	NNS
cord-021063-4y8m33ea	105	10	of	of	IN
cord-021063-4y8m33ea	105	11	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	105	12	.	.	.
cord-021063-4y8m33ea	106	1	Because	because	IN
cord-021063-4y8m33ea	106	2	they	-PRON-	PRP
cord-021063-4y8m33ea	106	3	can	can	MD
cord-021063-4y8m33ea	106	4	be	be	VB
cord-021063-4y8m33ea	106	5	made	make	VBN
cord-021063-4y8m33ea	106	6	from	from	IN
cord-021063-4y8m33ea	106	7	completely	completely	RB
cord-021063-4y8m33ea	106	8	pure	pure	JJ
cord-021063-4y8m33ea	106	9	components	component	NNS
cord-021063-4y8m33ea	106	10	,	,	,
cord-021063-4y8m33ea	106	11	they	-PRON-	PRP
cord-021063-4y8m33ea	106	12	have	have	VBP
cord-021063-4y8m33ea	106	13	been	be	VBN
cord-021063-4y8m33ea	106	14	used	use	VBN
cord-021063-4y8m33ea	106	15	extensively	extensively	RB
cord-021063-4y8m33ea	106	16	to	to	TO
cord-021063-4y8m33ea	106	17	characterize	characterize	VB
cord-021063-4y8m33ea	106	18	and	and	CC
cord-021063-4y8m33ea	106	19	define	define	VB
cord-021063-4y8m33ea	106	20	the	the	DT
cord-021063-4y8m33ea	106	21	physical	physical	JJ
cord-021063-4y8m33ea	106	22	properties	property	NNS
cord-021063-4y8m33ea	106	23	of	of	IN
cord-021063-4y8m33ea	106	24	membranes	membrane	NNS
cord-021063-4y8m33ea	106	25	and	and	CC
cord-021063-4y8m33ea	106	26	membrane	membrane	NN
cord-021063-4y8m33ea	106	27	proteins	protein	NNS
cord-021063-4y8m33ea	106	28	.	.	.
cord-021063-4y8m33ea	107	1	Liposomes	liposome	NNS
cord-021063-4y8m33ea	107	2	have	have	VBP
cord-021063-4y8m33ea	107	3	also	also	RB
cord-021063-4y8m33ea	107	4	been	be	VBN
cord-021063-4y8m33ea	107	5	used	use	VBN
cord-021063-4y8m33ea	107	6	as	as	IN
cord-021063-4y8m33ea	107	7	a	a	DT
cord-021063-4y8m33ea	107	8	means	means	NN
cord-021063-4y8m33ea	107	9	of	of	IN
cord-021063-4y8m33ea	107	10	delivering	deliver	VBG
cord-021063-4y8m33ea	107	11	substances	substance	NNS
cord-021063-4y8m33ea	107	12	to	to	IN
cord-021063-4y8m33ea	107	13	cells	cell	NNS
cord-021063-4y8m33ea	107	14	,	,	,
cord-021063-4y8m33ea	107	15	both	both	CC
cord-021063-4y8m33ea	107	16	in	in	IN
cord-021063-4y8m33ea	107	17	vitro	vitro	FW
cord-021063-4y8m33ea	107	18	and	and	CC
cord-021063-4y8m33ea	107	19	in	in	IN
cord-021063-4y8m33ea	107	20	vivo	vivo	NN
cord-021063-4y8m33ea	107	21	,	,	,
cord-021063-4y8m33ea	107	22	including	include	VBG
cord-021063-4y8m33ea	107	23	DNA	dna	NN
cord-021063-4y8m33ea	107	24	(	(	-LRB-
cord-021063-4y8m33ea	107	25	Hug	Hug	NNP
cord-021063-4y8m33ea	107	26	and	and	CC
cord-021063-4y8m33ea	107	27	Sleight	Sleight	NNP
cord-021063-4y8m33ea	107	28	,	,	,
cord-021063-4y8m33ea	107	29	1991	1991	CD
cord-021063-4y8m33ea	107	30	)	)	-RRB-
cord-021063-4y8m33ea	107	31	.	.	.
cord-021063-4y8m33ea	108	1	Before	before	IN
cord-021063-4y8m33ea	108	2	discussing	discuss	VBG
cord-021063-4y8m33ea	108	3	factors	factor	NNS
cord-021063-4y8m33ea	108	4	to	to	TO
cord-021063-4y8m33ea	108	5	be	be	VB
cord-021063-4y8m33ea	108	6	considered	consider	VBN
cord-021063-4y8m33ea	108	7	in	in	IN
cord-021063-4y8m33ea	108	8	preparing	prepare	VBG
cord-021063-4y8m33ea	108	9	liposomes	liposome	NNS
cord-021063-4y8m33ea	108	10	for	for	IN
cord-021063-4y8m33ea	108	11	gene	gene	NN
cord-021063-4y8m33ea	108	12	therapy	therapy	NN
cord-021063-4y8m33ea	108	13	of	of	IN
cord-021063-4y8m33ea	108	14	a	a	DT
cord-021063-4y8m33ea	108	15	given	give	VBN
cord-021063-4y8m33ea	108	16	disease	disease	NN
cord-021063-4y8m33ea	108	17	,	,	,
cord-021063-4y8m33ea	108	18	it	-PRON-	PRP
cord-021063-4y8m33ea	108	19	is	be	VBZ
cord-021063-4y8m33ea	108	20	worthwhile	worthwhile	JJ
cord-021063-4y8m33ea	108	21	to	to	TO
cord-021063-4y8m33ea	108	22	review	review	VB
cord-021063-4y8m33ea	108	23	some	some	DT
cord-021063-4y8m33ea	108	24	general	general	JJ
cord-021063-4y8m33ea	108	25	aspects	aspect	NNS
cord-021063-4y8m33ea	108	26	of	of	IN
cord-021063-4y8m33ea	108	27	liposome	liposome	NN
cord-021063-4y8m33ea	108	28	preparation	preparation	NN
cord-021063-4y8m33ea	108	29	and	and	CC
cord-021063-4y8m33ea	108	30	design	design	NN
cord-021063-4y8m33ea	108	31	.	.	.
cord-021063-4y8m33ea	109	1	Liposomes	liposome	NNS
cord-021063-4y8m33ea	109	2	are	be	VBP
cord-021063-4y8m33ea	109	3	generally	generally	RB
cord-021063-4y8m33ea	109	4	classified	classify	VBN
cord-021063-4y8m33ea	109	5	by	by	IN
cord-021063-4y8m33ea	109	6	the	the	DT
cord-021063-4y8m33ea	109	7	number	number	NN
cord-021063-4y8m33ea	109	8	of	of	IN
cord-021063-4y8m33ea	109	9	bilayers	bilayer	NNS
cord-021063-4y8m33ea	109	10	(	(	-LRB-
cord-021063-4y8m33ea	109	11	lamellae	lamella	NNS
cord-021063-4y8m33ea	109	12	)	)	-RRB-
cord-021063-4y8m33ea	109	13	they	-PRON-	PRP
cord-021063-4y8m33ea	109	14	contain	contain	VBP
cord-021063-4y8m33ea	109	15	,	,	,
cord-021063-4y8m33ea	109	16	by	by	IN
cord-021063-4y8m33ea	109	17	size	size	NN
cord-021063-4y8m33ea	109	18	,	,	,
cord-021063-4y8m33ea	109	19	and	and	CC
cord-021063-4y8m33ea	109	20	by	by	IN
cord-021063-4y8m33ea	109	21	the	the	DT
cord-021063-4y8m33ea	109	22	composition	composition	NN
cord-021063-4y8m33ea	109	23	of	of	IN
cord-021063-4y8m33ea	109	24	their	-PRON-	PRP$
cord-021063-4y8m33ea	109	25	membrane	membrane	NN
cord-021063-4y8m33ea	109	26	.	.	.
cord-021063-4y8m33ea	110	1	The	the	DT
cord-021063-4y8m33ea	110	2	earliest	early	JJS
cord-021063-4y8m33ea	110	3	liposomes	liposome	NNS
cord-021063-4y8m33ea	110	4	were	be	VBD
cord-021063-4y8m33ea	110	5	formed	form	VBN
cord-021063-4y8m33ea	110	6	by	by	IN
cord-021063-4y8m33ea	110	7	hydration	hydration	NN
cord-021063-4y8m33ea	110	8	of	of	IN
cord-021063-4y8m33ea	110	9	a	a	DT
cord-021063-4y8m33ea	110	10	dried	dry	VBN
cord-021063-4y8m33ea	110	11	lipid	lipid	NN
cord-021063-4y8m33ea	110	12	film	film	NN
cord-021063-4y8m33ea	110	13	,	,	,
cord-021063-4y8m33ea	110	14	followed	follow	VBN
cord-021063-4y8m33ea	110	15	by	by	IN
cord-021063-4y8m33ea	110	16	vortexing	vortexe	VBG
cord-021063-4y8m33ea	110	17	.	.	.
cord-021063-4y8m33ea	111	1	This	this	DT
cord-021063-4y8m33ea	111	2	results	result	VBZ
cord-021063-4y8m33ea	111	3	in	in	IN
cord-021063-4y8m33ea	111	4	a	a	DT
cord-021063-4y8m33ea	111	5	cloudy	cloudy	JJ
cord-021063-4y8m33ea	111	6	solution	solution	NN
cord-021063-4y8m33ea	111	7	of	of	IN
cord-021063-4y8m33ea	111	8	liposomes	liposome	NNS
cord-021063-4y8m33ea	111	9	having	have	VBG
cord-021063-4y8m33ea	111	10	many	many	JJ
cord-021063-4y8m33ea	111	11	lamellar	lamellar	JJ
cord-021063-4y8m33ea	111	12	layers	layer	NNS
cord-021063-4y8m33ea	111	13	,	,	,
cord-021063-4y8m33ea	111	14	resembling	resemble	VBG
cord-021063-4y8m33ea	111	15	an	an	DT
cord-021063-4y8m33ea	111	16	onion	onion	NN
cord-021063-4y8m33ea	111	17	.	.	.
cord-021063-4y8m33ea	112	1	These	these	DT
cord-021063-4y8m33ea	112	2	are	be	VBP
cord-021063-4y8m33ea	112	3	called	call	VBN
cord-021063-4y8m33ea	112	4	multilamellar	multilamellar	JJ
cord-021063-4y8m33ea	112	5	vesicles	vesicle	NNS
cord-021063-4y8m33ea	112	6	,	,	,
cord-021063-4y8m33ea	112	7	or	or	CC
cord-021063-4y8m33ea	112	8	MLVs	mlv	NNS
cord-021063-4y8m33ea	112	9	.	.	.
cord-021063-4y8m33ea	113	1	Although	although	IN
cord-021063-4y8m33ea	113	2	MLVs	mlv	NNS
cord-021063-4y8m33ea	113	3	are	be	VBP
cord-021063-4y8m33ea	113	4	effective	effective	JJ
cord-021063-4y8m33ea	113	5	for	for	IN
cord-021063-4y8m33ea	113	6	the	the	DT
cord-021063-4y8m33ea	113	7	packaging	packaging	NN
cord-021063-4y8m33ea	113	8	of	of	IN
cord-021063-4y8m33ea	113	9	lipophilic	lipophilic	JJ
cord-021063-4y8m33ea	113	10	drugs	drug	NNS
cord-021063-4y8m33ea	113	11	,	,	,
cord-021063-4y8m33ea	113	12	they	-PRON-	PRP
cord-021063-4y8m33ea	113	13	have	have	VBP
cord-021063-4y8m33ea	113	14	very	very	RB
cord-021063-4y8m33ea	113	15	little	little	JJ
cord-021063-4y8m33ea	113	16	aqueous	aqueous	JJ
cord-021063-4y8m33ea	113	17	volume	volume	NN
cord-021063-4y8m33ea	113	18	in	in	IN
cord-021063-4y8m33ea	113	19	their	-PRON-	PRP$
cord-021063-4y8m33ea	113	20	interiors	interior	NNS
cord-021063-4y8m33ea	113	21	,	,	,
cord-021063-4y8m33ea	113	22	and	and	CC
cord-021063-4y8m33ea	113	23	are	be	VBP
cord-021063-4y8m33ea	113	24	not	not	RB
cord-021063-4y8m33ea	113	25	efficient	efficient	JJ
cord-021063-4y8m33ea	113	26	at	at	IN
cord-021063-4y8m33ea	113	27	entrapping	entrap	VBG
cord-021063-4y8m33ea	113	28	water	water	NN
cord-021063-4y8m33ea	113	29	-	-	HYPH
cord-021063-4y8m33ea	113	30	soluble	soluble	JJ
cord-021063-4y8m33ea	113	31	compounds	compound	NNS
cord-021063-4y8m33ea	113	32	or	or	CC
cord-021063-4y8m33ea	113	33	large	large	JJ
cord-021063-4y8m33ea	113	34	DNA	dna	NN
cord-021063-4y8m33ea	113	35	fragments	fragment	NNS
cord-021063-4y8m33ea	113	36	.	.	.
cord-021063-4y8m33ea	114	1	Because	because	IN
cord-021063-4y8m33ea	114	2	of	of	IN
cord-021063-4y8m33ea	114	3	these	these	DT
cord-021063-4y8m33ea	114	4	deficiencies	deficiency	NNS
cord-021063-4y8m33ea	114	5	,	,	,
cord-021063-4y8m33ea	114	6	MLVs	mlv	NNS
cord-021063-4y8m33ea	114	7	were	be	VBD
cord-021063-4y8m33ea	114	8	quickly	quickly	RB
cord-021063-4y8m33ea	114	9	superseded	supersede	VBN
cord-021063-4y8m33ea	114	10	by	by	IN
cord-021063-4y8m33ea	114	11	unilamellar	unilamellar	NNP
cord-021063-4y8m33ea	114	12	vesicles	vesicle	NNS
cord-021063-4y8m33ea	114	13	,	,	,
cord-021063-4y8m33ea	114	14	which	which	WDT
cord-021063-4y8m33ea	114	15	have	have	VBP
cord-021063-4y8m33ea	114	16	only	only	RB
cord-021063-4y8m33ea	114	17	a	a	DT
cord-021063-4y8m33ea	114	18	single	single	JJ
cord-021063-4y8m33ea	114	19	lipid	lipid	NN
cord-021063-4y8m33ea	114	20	bilayer	bilayer	NN
cord-021063-4y8m33ea	114	21	between	between	IN
cord-021063-4y8m33ea	114	22	the	the	DT
cord-021063-4y8m33ea	114	23	external	external	JJ
cord-021063-4y8m33ea	114	24	fluid	fluid	NN
cord-021063-4y8m33ea	114	25	and	and	CC
cord-021063-4y8m33ea	114	26	the	the	DT
cord-021063-4y8m33ea	114	27	lumen	lumen	NN
cord-021063-4y8m33ea	114	28	.	.	.
cord-021063-4y8m33ea	115	1	Several	several	JJ
cord-021063-4y8m33ea	115	2	methods	method	NNS
cord-021063-4y8m33ea	115	3	are	be	VBP
cord-021063-4y8m33ea	115	4	used	use	VBN
cord-021063-4y8m33ea	115	5	to	to	TO
cord-021063-4y8m33ea	115	6	pre	pre	VB
cord-021063-4y8m33ea	115	7	~	~	NNP
cord-021063-4y8m33ea	115	8	pare	pare	VB
cord-021063-4y8m33ea	115	9	unilamellar	unilamellar	NNP
cord-021063-4y8m33ea	115	10	vesicles	vesicle	NNS
cord-021063-4y8m33ea	115	11	,	,	,
cord-021063-4y8m33ea	115	12	each	each	DT
cord-021063-4y8m33ea	115	13	having	have	VBG
cord-021063-4y8m33ea	115	14	its	-PRON-	PRP$
cord-021063-4y8m33ea	115	15	own	own	JJ
cord-021063-4y8m33ea	115	16	advantages	advantage	NNS
cord-021063-4y8m33ea	115	17	and	and	CC
cord-021063-4y8m33ea	115	18	disadvantages	disadvantage	NNS
cord-021063-4y8m33ea	115	19	.	.	.
cord-021063-4y8m33ea	116	1	Because	because	IN
cord-021063-4y8m33ea	116	2	of	of	IN
cord-021063-4y8m33ea	116	3	space	space	NN
cord-021063-4y8m33ea	116	4	limitations	limitation	NNS
cord-021063-4y8m33ea	116	5	,	,	,
cord-021063-4y8m33ea	116	6	we	-PRON-	PRP
cord-021063-4y8m33ea	116	7	will	will	MD
cord-021063-4y8m33ea	116	8	only	only	RB
cord-021063-4y8m33ea	116	9	briefly	briefly	RB
cord-021063-4y8m33ea	116	10	describe	describe	VB
cord-021063-4y8m33ea	116	11	those	those	DT
cord-021063-4y8m33ea	116	12	methods	method	NNS
cord-021063-4y8m33ea	116	13	of	of	IN
cord-021063-4y8m33ea	116	14	liposome	liposome	NN
cord-021063-4y8m33ea	116	15	preparation	preparation	NN
cord-021063-4y8m33ea	116	16	that	that	WDT
cord-021063-4y8m33ea	116	17	are	be	VBP
cord-021063-4y8m33ea	116	18	directly	directly	RB
cord-021063-4y8m33ea	116	19	relevant	relevant	JJ
cord-021063-4y8m33ea	116	20	to	to	IN
cord-021063-4y8m33ea	116	21	the	the	DT
cord-021063-4y8m33ea	116	22	construction	construction	NN
cord-021063-4y8m33ea	116	23	of	of	IN
cord-021063-4y8m33ea	116	24	gene	gene	NN
cord-021063-4y8m33ea	116	25	therapy	therapy	NN
cord-021063-4y8m33ea	116	26	vectors	vector	NNS
cord-021063-4y8m33ea	116	27	.	.	.
cord-021063-4y8m33ea	117	1	Table	table	NN
cord-021063-4y8m33ea	117	2	3	3	CD
cord-021063-4y8m33ea	117	3	gives	give	VBZ
cord-021063-4y8m33ea	117	4	a	a	DT
cord-021063-4y8m33ea	117	5	direct	direct	JJ
cord-021063-4y8m33ea	117	6	comparison	comparison	NN
cord-021063-4y8m33ea	117	7	of	of	IN
cord-021063-4y8m33ea	117	8	these	these	DT
cord-021063-4y8m33ea	117	9	methods	method	NNS
cord-021063-4y8m33ea	117	10	of	of	IN
cord-021063-4y8m33ea	117	11	preparation	preparation	NN
cord-021063-4y8m33ea	117	12	as	as	IN
cord-021063-4y8m33ea	117	13	related	relate	VBN
cord-021063-4y8m33ea	117	14	to	to	IN
cord-021063-4y8m33ea	117	15	transfection	transfection	NN
cord-021063-4y8m33ea	117	16	.	.	.
cord-021063-4y8m33ea	118	1	Cationic	Cationic	NNP
cord-021063-4y8m33ea	118	2	Liposomes	liposome	NNS
cord-021063-4y8m33ea	118	3	.	.	.
cord-021063-4y8m33ea	119	1	Liposomes	liposome	NNS
cord-021063-4y8m33ea	119	2	made	make	VBD
cord-021063-4y8m33ea	119	3	entirely	entirely	RB
cord-021063-4y8m33ea	119	4	or	or	CC
cord-021063-4y8m33ea	119	5	partially	partially	RB
cord-021063-4y8m33ea	119	6	of	of	IN
cord-021063-4y8m33ea	119	7	a	a	DT
cord-021063-4y8m33ea	119	8	cationic	cationic	JJ
cord-021063-4y8m33ea	119	9	lipid	lipid	NN
cord-021063-4y8m33ea	119	10	have	have	VBP
cord-021063-4y8m33ea	119	11	a	a	DT
cord-021063-4y8m33ea	119	12	charge	charge	NN
cord-021063-4y8m33ea	119	13	-	-	HYPH
cord-021063-4y8m33ea	119	14	based	base	VBN
cord-021063-4y8m33ea	119	15	affinity	affinity	NN
cord-021063-4y8m33ea	119	16	for	for	IN
cord-021063-4y8m33ea	119	17	DNA	dna	NN
cord-021063-4y8m33ea	119	18	.	.	.
cord-021063-4y8m33ea	120	1	When	when	WRB
cord-021063-4y8m33ea	120	2	such	such	JJ
cord-021063-4y8m33ea	120	3	liposomes	liposome	NNS
cord-021063-4y8m33ea	120	4	are	be	VBP
cord-021063-4y8m33ea	120	5	incubated	incubate	VBN
cord-021063-4y8m33ea	120	6	in	in	IN
cord-021063-4y8m33ea	120	7	solution	solution	NN
cord-021063-4y8m33ea	120	8	with	with	IN
cord-021063-4y8m33ea	120	9	DNA	DNA	NNP
cord-021063-4y8m33ea	120	10	,	,	,
cord-021063-4y8m33ea	120	11	they	-PRON-	PRP
cord-021063-4y8m33ea	120	12	form	form	VBP
cord-021063-4y8m33ea	120	13	complexes	complex	NNS
cord-021063-4y8m33ea	120	14	that	that	WDT
cord-021063-4y8m33ea	120	15	can	can	MD
cord-021063-4y8m33ea	120	16	transfect	transfect	VB
cord-021063-4y8m33ea	120	17	cells	cell	NNS
cord-021063-4y8m33ea	120	18	.	.	.
cord-021063-4y8m33ea	121	1	Several	several	JJ
cord-021063-4y8m33ea	121	2	types	type	NNS
cord-021063-4y8m33ea	121	3	of	of	IN
cord-021063-4y8m33ea	121	4	cationic	cationic	JJ
cord-021063-4y8m33ea	121	5	lipid	lipid	NN
cord-021063-4y8m33ea	121	6	are	be	VBP
cord-021063-4y8m33ea	121	7	available	available	JJ
cord-021063-4y8m33ea	121	8	which	which	WDT
cord-021063-4y8m33ea	121	9	in	in	IN
cord-021063-4y8m33ea	121	10	general	general	JJ
cord-021063-4y8m33ea	121	11	have	have	VBP
cord-021063-4y8m33ea	121	12	similar	similar	JJ
cord-021063-4y8m33ea	121	13	characteristics	characteristic	NNS
cord-021063-4y8m33ea	121	14	(	(	-LRB-
cord-021063-4y8m33ea	121	15	Leventis	Leventis	NNP
cord-021063-4y8m33ea	121	16	and	and	CC
cord-021063-4y8m33ea	121	17	Silvius	Silvius	NNP
cord-021063-4y8m33ea	121	18	,	,	,
cord-021063-4y8m33ea	121	19	1990	1990	CD
cord-021063-4y8m33ea	121	20	;	;	:
cord-021063-4y8m33ea	121	21	Ito	Ito	NNP
cord-021063-4y8m33ea	121	22	et	et	NNP
cord-021063-4y8m33ea	121	23	al	al	NNP
cord-021063-4y8m33ea	121	24	.	.	NNP
cord-021063-4y8m33ea	121	25	,	,	,
cord-021063-4y8m33ea	121	26	1990	1990	CD
cord-021063-4y8m33ea	121	27	;	;	:
cord-021063-4y8m33ea	121	28	Gao	Gao	NNP
cord-021063-4y8m33ea	121	29	and	and	CC
cord-021063-4y8m33ea	121	30	Huang	Huang	NNP
cord-021063-4y8m33ea	121	31	,	,	,
cord-021063-4y8m33ea	121	32	1991	1991	CD
cord-021063-4y8m33ea	121	33	;	;	:
cord-021063-4y8m33ea	121	34	Hazinski	Hazinski	NNP
cord-021063-4y8m33ea	121	35	et	et	NNP
cord-021063-4y8m33ea	121	36	al	al	NNP
cord-021063-4y8m33ea	121	37	.	.	NNP
cord-021063-4y8m33ea	121	38	,	,	,
cord-021063-4y8m33ea	121	39	1991	1991	CD
cord-021063-4y8m33ea	121	40	;	;	:
cord-021063-4y8m33ea	121	41	Farhood	Farhood	NNP
cord-021063-4y8m33ea	121	42	et	et	NNP
cord-021063-4y8m33ea	121	43	al	al	NNP
cord-021063-4y8m33ea	121	44	.	.	NNP
cord-021063-4y8m33ea	121	45	,	,	,
cord-021063-4y8m33ea	121	46	1992	1992	CD
cord-021063-4y8m33ea	121	47	)	)	-RRB-
cord-021063-4y8m33ea	121	48	.	.	.
cord-021063-4y8m33ea	122	1	This	this	DT
cord-021063-4y8m33ea	122	2	technique	technique	NN
cord-021063-4y8m33ea	122	3	was	be	VBD
cord-021063-4y8m33ea	122	4	initially	initially	RB
cord-021063-4y8m33ea	122	5	intended	intend	VBN
cord-021063-4y8m33ea	122	6	exclusively	exclusively	RB
cord-021063-4y8m33ea	122	7	for	for	IN
cord-021063-4y8m33ea	122	8	in	in	FW
cord-021063-4y8m33ea	122	9	vitro	vitro	FW
cord-021063-4y8m33ea	122	10	use	use	NN
cord-021063-4y8m33ea	122	11	.	.	.
cord-021063-4y8m33ea	123	1	However	however	RB
cord-021063-4y8m33ea	123	2	,	,	,
cord-021063-4y8m33ea	123	3	in	in	IN
cord-021063-4y8m33ea	123	4	vivo	vivo	FW
cord-021063-4y8m33ea	123	5	transfections	transfection	NNS
cord-021063-4y8m33ea	123	6	have	have	VBP
cord-021063-4y8m33ea	123	7	been	be	VBN
cord-021063-4y8m33ea	123	8	performed	perform	VBN
cord-021063-4y8m33ea	123	9	with	with	IN
cord-021063-4y8m33ea	123	10	several	several	JJ
cord-021063-4y8m33ea	123	11	commercial	commercial	JJ
cord-021063-4y8m33ea	123	12	preparations	preparation	NNS
cord-021063-4y8m33ea	123	13	of	of	IN
cord-021063-4y8m33ea	123	14	cationic	cationic	JJ
cord-021063-4y8m33ea	123	15	lipids	lipid	NNS
cord-021063-4y8m33ea	123	16	.	.	.
cord-021063-4y8m33ea	124	1	There	there	EX
cord-021063-4y8m33ea	124	2	are	be	VBP
cord-021063-4y8m33ea	124	3	several	several	JJ
cord-021063-4y8m33ea	124	4	reasons	reason	NNS
cord-021063-4y8m33ea	124	5	why	why	WRB
cord-021063-4y8m33ea	124	6	cationic	cationic	JJ
cord-021063-4y8m33ea	124	7	lipid	lipid	NN
cord-021063-4y8m33ea	124	8	-	-	HYPH
cord-021063-4y8m33ea	124	9	DNA	dna	NN
cord-021063-4y8m33ea	124	10	complexes	complex	NNS
cord-021063-4y8m33ea	124	11	have	have	VBP
cord-021063-4y8m33ea	124	12	proved	prove	VBN
cord-021063-4y8m33ea	124	13	so	so	RB
cord-021063-4y8m33ea	124	14	popular	popular	JJ
cord-021063-4y8m33ea	124	15	for	for	IN
cord-021063-4y8m33ea	124	16	transfections	transfection	NNS
cord-021063-4y8m33ea	124	17	:	:	:
cord-021063-4y8m33ea	125	1	(	(	-LRB-
cord-021063-4y8m33ea	125	2	i	i	NN
cord-021063-4y8m33ea	125	3	)	)	-RRB-
cord-021063-4y8m33ea	126	1	They	-PRON-	PRP
cord-021063-4y8m33ea	126	2	are	be	VBP
cord-021063-4y8m33ea	126	3	very	very	RB
cord-021063-4y8m33ea	126	4	easy	easy	JJ
cord-021063-4y8m33ea	126	5	to	to	TO
cord-021063-4y8m33ea	126	6	prepare	prepare	VB
cord-021063-4y8m33ea	126	7	.	.	.
cord-021063-4y8m33ea	127	1	The	the	DT
cord-021063-4y8m33ea	127	2	liposomes	liposome	NNS
cord-021063-4y8m33ea	127	3	and	and	CC
cord-021063-4y8m33ea	127	4	the	the	DT
cord-021063-4y8m33ea	127	5	DNA	dna	NN
cord-021063-4y8m33ea	127	6	are	be	VBP
cord-021063-4y8m33ea	127	7	incubated	incubate	VBN
cord-021063-4y8m33ea	127	8	together	together	RB
cord-021063-4y8m33ea	127	9	,	,	,
cord-021063-4y8m33ea	127	10	and	and	CC
cord-021063-4y8m33ea	127	11	then	then	RB
cord-021063-4y8m33ea	127	12	used	use	VBN
cord-021063-4y8m33ea	127	13	without	without	IN
cord-021063-4y8m33ea	127	14	further	further	JJ
cord-021063-4y8m33ea	127	15	preparation	preparation	NN
cord-021063-4y8m33ea	127	16	.	.	.
cord-021063-4y8m33ea	128	1	(	(	-LRB-
cord-021063-4y8m33ea	128	2	ii	ii	NN
cord-021063-4y8m33ea	128	3	)	)	-RRB-
cord-021063-4y8m33ea	129	1	The	the	DT
cord-021063-4y8m33ea	129	2	complexes	complex	NNS
cord-021063-4y8m33ea	129	3	are	be	VBP
cord-021063-4y8m33ea	129	4	relatively	relatively	RB
cord-021063-4y8m33ea	129	5	non	non	JJ
cord-021063-4y8m33ea	129	6	-	-	JJ
cord-021063-4y8m33ea	129	7	toxic	toxic	JJ
cord-021063-4y8m33ea	129	8	.	.	.
cord-021063-4y8m33ea	130	1	(	(	-LRB-
cord-021063-4y8m33ea	130	2	iii	iii	NN
cord-021063-4y8m33ea	130	3	)	)	-RRB-
cord-021063-4y8m33ea	131	1	Transfections	transfection	NNS
cord-021063-4y8m33ea	131	2	using	use	VBG
cord-021063-4y8m33ea	131	3	this	this	DT
cord-021063-4y8m33ea	131	4	method	method	NN
cord-021063-4y8m33ea	131	5	are	be	VBP
cord-021063-4y8m33ea	131	6	efficient	efficient	JJ
cord-021063-4y8m33ea	131	7	with	with	IN
cord-021063-4y8m33ea	131	8	most	most	JJS
cord-021063-4y8m33ea	131	9	cell	cell	NN
cord-021063-4y8m33ea	131	10	types	type	NNS
cord-021063-4y8m33ea	131	11	.	.	.
cord-021063-4y8m33ea	132	1	And	and	CC
cord-021063-4y8m33ea	132	2	(	(	-LRB-
cord-021063-4y8m33ea	132	3	iv	iv	LS
cord-021063-4y8m33ea	132	4	)	)	-RRB-
cord-021063-4y8m33ea	132	5	because	because	IN
cord-021063-4y8m33ea	132	6	the	the	DT
cord-021063-4y8m33ea	132	7	DNA	dna	NN
cord-021063-4y8m33ea	132	8	remains	remain	VBZ
cord-021063-4y8m33ea	132	9	outside	outside	IN
cord-021063-4y8m33ea	132	10	the	the	DT
cord-021063-4y8m33ea	132	11	liposome	liposome	NN
cord-021063-4y8m33ea	132	12	,	,	,
cord-021063-4y8m33ea	132	13	questions	question	NNS
cord-021063-4y8m33ea	132	14	of	of	IN
cord-021063-4y8m33ea	132	15	entrapment	entrapment	NN
cord-021063-4y8m33ea	132	16	efficiency	efficiency	NN
cord-021063-4y8m33ea	132	17	do	do	VBP
cord-021063-4y8m33ea	132	18	not	not	RB
cord-021063-4y8m33ea	132	19	apply	apply	VB
cord-021063-4y8m33ea	132	20	.	.	.
cord-021063-4y8m33ea	133	1	In	in	IN
cord-021063-4y8m33ea	133	2	vivo	vivo	FW
cord-021063-4y8m33ea	133	3	transfections	transfection	NNS
cord-021063-4y8m33ea	133	4	using	use	VBG
cord-021063-4y8m33ea	133	5	cationic	cationic	JJ
cord-021063-4y8m33ea	133	6	lipid	lipid	NN
cord-021063-4y8m33ea	133	7	-	-	HYPH
cord-021063-4y8m33ea	133	8	DNA	dna	NN
cord-021063-4y8m33ea	133	9	complexes	complex	NNS
cord-021063-4y8m33ea	133	10	are	be	VBP
cord-021063-4y8m33ea	133	11	completely	completely	RB
cord-021063-4y8m33ea	133	12	uncontrolled	uncontrolled	JJ
cord-021063-4y8m33ea	133	13	.	.	.
cord-021063-4y8m33ea	134	1	That	that	RB
cord-021063-4y8m33ea	134	2	is	is	RB
cord-021063-4y8m33ea	134	3	,	,	,
cord-021063-4y8m33ea	134	4	there	there	EX
cord-021063-4y8m33ea	134	5	are	be	VBP
cord-021063-4y8m33ea	134	6	no	no	DT
cord-021063-4y8m33ea	134	7	targeting	targeting	JJ
cord-021063-4y8m33ea	134	8	or	or	CC
cord-021063-4y8m33ea	134	9	specific	specific	JJ
cord-021063-4y8m33ea	134	10	binding	bind	VBG
cord-021063-4y8m33ea	134	11	agents	agent	NNS
cord-021063-4y8m33ea	134	12	present	present	JJ
cord-021063-4y8m33ea	134	13	,	,	,
cord-021063-4y8m33ea	134	14	and	and	CC
cord-021063-4y8m33ea	134	15	the	the	DT
cord-021063-4y8m33ea	134	16	complexes	complex	NNS
cord-021063-4y8m33ea	134	17	probably	probably	RB
cord-021063-4y8m33ea	134	18	deliver	deliver	VBP
cord-021063-4y8m33ea	134	19	DNA	DNA	NNP
cord-021063-4y8m33ea	134	20	to	to	IN
cord-021063-4y8m33ea	134	21	the	the	DT
cord-021063-4y8m33ea	134	22	first	first	JJ
cord-021063-4y8m33ea	134	23	cell	cell	NN
cord-021063-4y8m33ea	134	24	they	-PRON-	PRP
cord-021063-4y8m33ea	134	25	contact	contact	VBP
cord-021063-4y8m33ea	134	26	.	.	.
cord-021063-4y8m33ea	135	1	This	this	DT
cord-021063-4y8m33ea	135	2	occurs	occur	VBZ
cord-021063-4y8m33ea	135	3	most	most	RBS
cord-021063-4y8m33ea	135	4	often	often	RB
cord-021063-4y8m33ea	135	5	in	in	IN
cord-021063-4y8m33ea	135	6	the	the	DT
cord-021063-4y8m33ea	135	7	first	first	JJ
cord-021063-4y8m33ea	135	8	capillary	capillary	JJ
cord-021063-4y8m33ea	135	9	bed	bed	NN
cord-021063-4y8m33ea	135	10	through	through	IN
cord-021063-4y8m33ea	135	11	which	which	WDT
cord-021063-4y8m33ea	135	12	the	the	DT
cord-021063-4y8m33ea	135	13	injected	inject	VBN
cord-021063-4y8m33ea	135	14	complexes	complex	NNS
cord-021063-4y8m33ea	135	15	pass	pass	VBP
cord-021063-4y8m33ea	135	16	.	.	.
cord-021063-4y8m33ea	136	1	When	when	WRB
cord-021063-4y8m33ea	136	2	DNA	dna	NN
cord-021063-4y8m33ea	136	3	-	-	HYPH
cord-021063-4y8m33ea	136	4	lipid	lipid	NN
cord-021063-4y8m33ea	136	5	complexes	complex	NNS
cord-021063-4y8m33ea	136	6	are	be	VBP
cord-021063-4y8m33ea	136	7	intravenously	intravenously	RB
cord-021063-4y8m33ea	136	8	injected	inject	VBN
cord-021063-4y8m33ea	136	9	,	,	,
cord-021063-4y8m33ea	136	10	the	the	DT
cord-021063-4y8m33ea	136	11	highest	high	JJS
cord-021063-4y8m33ea	136	12	levels	level	NNS
cord-021063-4y8m33ea	136	13	of	of	IN
cord-021063-4y8m33ea	136	14	gene	gene	NN
cord-021063-4y8m33ea	136	15	expression	expression	NN
cord-021063-4y8m33ea	136	16	are	be	VBP
cord-021063-4y8m33ea	136	17	usually	usually	RB
cord-021063-4y8m33ea	136	18	seen	see	VBN
cord-021063-4y8m33ea	136	19	in	in	IN
cord-021063-4y8m33ea	136	20	the	the	DT
cord-021063-4y8m33ea	136	21	pulmonary	pulmonary	JJ
cord-021063-4y8m33ea	136	22	vascular	vascular	JJ
cord-021063-4y8m33ea	136	23	endothelium	endothelium	NN
cord-021063-4y8m33ea	136	24	(	(	-LRB-
cord-021063-4y8m33ea	136	25	Brigham	Brigham	NNP
cord-021063-4y8m33ea	136	26	et	et	NNP
cord-021063-4y8m33ea	136	27	al	al	NNP
cord-021063-4y8m33ea	136	28	.	.	NNP
cord-021063-4y8m33ea	136	29	,	,	,
cord-021063-4y8m33ea	136	30	1993	1993	CD
cord-021063-4y8m33ea	136	31	)	)	-RRB-
cord-021063-4y8m33ea	136	32	.	.	.
cord-021063-4y8m33ea	137	1	DNA	dna	NN
cord-021063-4y8m33ea	137	2	-	-	HYPH
cord-021063-4y8m33ea	137	3	lipid	lipid	NN
cord-021063-4y8m33ea	137	4	complexes	complex	NNS
cord-021063-4y8m33ea	137	5	introduced	introduce	VBN
cord-021063-4y8m33ea	137	6	into	into	IN
cord-021063-4y8m33ea	137	7	the	the	DT
cord-021063-4y8m33ea	137	8	airway	airway	NN
cord-021063-4y8m33ea	137	9	cause	cause	NN
cord-021063-4y8m33ea	137	10	a	a	DT
cord-021063-4y8m33ea	137	11	generalized	generalized	JJ
cord-021063-4y8m33ea	137	12	transfection	transfection	NN
cord-021063-4y8m33ea	137	13	of	of	IN
cord-021063-4y8m33ea	137	14	the	the	DT
cord-021063-4y8m33ea	137	15	lung	lung	NN
cord-021063-4y8m33ea	137	16	.	.	.
cord-021063-4y8m33ea	138	1	Because	because	IN
cord-021063-4y8m33ea	138	2	the	the	DT
cord-021063-4y8m33ea	138	3	site	site	NN
cord-021063-4y8m33ea	138	4	of	of	IN
cord-021063-4y8m33ea	138	5	transfection	transfection	NN
cord-021063-4y8m33ea	138	6	is	be	VBZ
cord-021063-4y8m33ea	138	7	completely	completely	RB
cord-021063-4y8m33ea	138	8	uncontrolled	uncontrolled	JJ
cord-021063-4y8m33ea	138	9	,	,	,
cord-021063-4y8m33ea	138	10	expression	expression	NN
cord-021063-4y8m33ea	138	11	of	of	IN
cord-021063-4y8m33ea	138	12	the	the	DT
cord-021063-4y8m33ea	138	13	inserted	insert	VBN
cord-021063-4y8m33ea	138	14	gene	gene	NN
cord-021063-4y8m33ea	138	15	may	may	MD
cord-021063-4y8m33ea	138	16	need	need	VB
cord-021063-4y8m33ea	138	17	to	to	TO
cord-021063-4y8m33ea	138	18	be	be	VB
cord-021063-4y8m33ea	138	19	regulated	regulate	VBN
cord-021063-4y8m33ea	138	20	by	by	IN
cord-021063-4y8m33ea	138	21	means	mean	NNS
cord-021063-4y8m33ea	138	22	of	of	IN
cord-021063-4y8m33ea	138	23	tissue	tissue	NN
cord-021063-4y8m33ea	138	24	-	-	HYPH
cord-021063-4y8m33ea	138	25	specific	specific	JJ
cord-021063-4y8m33ea	138	26	promoters	promoter	NNS
cord-021063-4y8m33ea	138	27	(	(	-LRB-
cord-021063-4y8m33ea	138	28	Hazinski	Hazinski	NNP
cord-021063-4y8m33ea	138	29	et	et	NNP
cord-021063-4y8m33ea	138	30	al	al	NNP
cord-021063-4y8m33ea	138	31	.	.	NNP
cord-021063-4y8m33ea	138	32	,	,	,
cord-021063-4y8m33ea	138	33	1991	1991	CD
cord-021063-4y8m33ea	138	34	)	)	-RRB-
cord-021063-4y8m33ea	138	35	.	.	.
cord-021063-4y8m33ea	139	1	Cell	cell	NN
cord-021063-4y8m33ea	139	2	specific	specific	JJ
cord-021063-4y8m33ea	139	3	promoters	promoter	NNS
cord-021063-4y8m33ea	139	4	are	be	VBP
cord-021063-4y8m33ea	139	5	not	not	RB
cord-021063-4y8m33ea	139	6	available	available	JJ
cord-021063-4y8m33ea	139	7	for	for	IN
cord-021063-4y8m33ea	139	8	all	all	DT
cord-021063-4y8m33ea	139	9	tissues	tissue	NNS
cord-021063-4y8m33ea	139	10	,	,	,
cord-021063-4y8m33ea	139	11	and	and	CC
cord-021063-4y8m33ea	139	12	those	those	DT
cord-021063-4y8m33ea	139	13	currently	currently	RB
cord-021063-4y8m33ea	139	14	available	available	JJ
cord-021063-4y8m33ea	139	15	are	be	VBP
cord-021063-4y8m33ea	139	16	sometimes	sometimes	RB
cord-021063-4y8m33ea	139	17	less	less	RBR
cord-021063-4y8m33ea	139	18	specific	specific	JJ
cord-021063-4y8m33ea	139	19	than	than	IN
cord-021063-4y8m33ea	139	20	desired	desire	VBN
cord-021063-4y8m33ea	139	21	.	.	.
cord-021063-4y8m33ea	140	1	While	while	IN
cord-021063-4y8m33ea	140	2	both	both	DT
cord-021063-4y8m33ea	140	3	cationic	cationic	JJ
cord-021063-4y8m33ea	140	4	liposomes	liposome	NNS
cord-021063-4y8m33ea	140	5	and	and	CC
cord-021063-4y8m33ea	140	6	retroviral	retroviral	NN
cord-021063-4y8m33ea	140	7	gene	gene	NN
cord-021063-4y8m33ea	140	8	delivery	delivery	NN
cord-021063-4y8m33ea	140	9	vectors	vector	NNS
cord-021063-4y8m33ea	140	10	lack	lack	VBP
cord-021063-4y8m33ea	140	11	the	the	DT
cord-021063-4y8m33ea	140	12	ability	ability	NN
cord-021063-4y8m33ea	140	13	to	to	TO
cord-021063-4y8m33ea	140	14	target	target	VB
cord-021063-4y8m33ea	140	15	specific	specific	JJ
cord-021063-4y8m33ea	140	16	cells	cell	NNS
cord-021063-4y8m33ea	140	17	,	,	,
cord-021063-4y8m33ea	140	18	the	the	DT
cord-021063-4y8m33ea	140	19	lack	lack	NN
cord-021063-4y8m33ea	140	20	of	of	IN
cord-021063-4y8m33ea	140	21	length	length	NN
cord-021063-4y8m33ea	140	22	constraints	constraint	NNS
cord-021063-4y8m33ea	140	23	makes	make	VBZ
cord-021063-4y8m33ea	140	24	tissue	tissue	NN
cord-021063-4y8m33ea	140	25	-	-	HYPH
cord-021063-4y8m33ea	140	26	specific	specific	JJ
cord-021063-4y8m33ea	140	27	expression	expression	NN
cord-021063-4y8m33ea	140	28	easier	easy	JJR
cord-021063-4y8m33ea	140	29	to	to	TO
cord-021063-4y8m33ea	140	30	achieve	achieve	VB
cord-021063-4y8m33ea	140	31	using	use	VBG
cord-021063-4y8m33ea	140	32	DNA	dna	NN
cord-021063-4y8m33ea	140	33	-	-	HYPH
cord-021063-4y8m33ea	140	34	lipid	lipid	NN
cord-021063-4y8m33ea	140	35	complexes	complex	NNS
cord-021063-4y8m33ea	140	36	as	as	IN
cord-021063-4y8m33ea	140	37	compared	compare	VBN
cord-021063-4y8m33ea	140	38	to	to	IN
cord-021063-4y8m33ea	140	39	retroviruses	retrovirus	NNS
cord-021063-4y8m33ea	140	40	.	.	.
cord-021063-4y8m33ea	141	1	Ca2	Ca2	NNP
cord-021063-4y8m33ea	141	2	+	+	CC
cord-021063-4y8m33ea	142	1	/EDTA	/EDTA	NFP
cord-021063-4y8m33ea	142	2	Chelation	Chelation	NNP
cord-021063-4y8m33ea	142	3	.	.	.
cord-021063-4y8m33ea	143	1	In	in	IN
cord-021063-4y8m33ea	143	2	the	the	DT
cord-021063-4y8m33ea	143	3	presence	presence	NN
cord-021063-4y8m33ea	143	4	of	of	IN
cord-021063-4y8m33ea	143	5	calcium	calcium	NN
cord-021063-4y8m33ea	143	6	,	,	,
cord-021063-4y8m33ea	143	7	small	small	JJ
cord-021063-4y8m33ea	143	8	unilamellar	unilamellar	NNP
cord-021063-4y8m33ea	143	9	vesicles	vesicle	NNS
cord-021063-4y8m33ea	143	10	(	(	-LRB-
cord-021063-4y8m33ea	143	11	SUVs	suv	NNS
cord-021063-4y8m33ea	143	12	)	)	-RRB-
cord-021063-4y8m33ea	143	13	composed	compose	VBN
cord-021063-4y8m33ea	143	14	of	of	IN
cord-021063-4y8m33ea	143	15	acidic	acidic	JJ
cord-021063-4y8m33ea	143	16	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	143	17	(	(	-LRB-
cord-021063-4y8m33ea	143	18	typically	typically	RB
cord-021063-4y8m33ea	143	19	phosphatidylserine	phosphatidylserine	NN
cord-021063-4y8m33ea	143	20	)	)	-RRB-
cord-021063-4y8m33ea	143	21	aggregate	aggregate	NN
cord-021063-4y8m33ea	143	22	and	and	CC
cord-021063-4y8m33ea	143	23	fuse	fuse	VB
cord-021063-4y8m33ea	143	24	into	into	IN
cord-021063-4y8m33ea	143	25	large	large	JJ
cord-021063-4y8m33ea	143	26	cochleate	cochleate	NN
cord-021063-4y8m33ea	143	27	structures	structure	NNS
cord-021063-4y8m33ea	143	28	.	.	.
cord-021063-4y8m33ea	144	1	When	when	WRB
cord-021063-4y8m33ea	144	2	EDTA	EDTA	NNP
cord-021063-4y8m33ea	144	3	is	be	VBZ
cord-021063-4y8m33ea	144	4	added	add	VBN
cord-021063-4y8m33ea	144	5	to	to	TO
cord-021063-4y8m33ea	144	6	chelate	chelate	VB
cord-021063-4y8m33ea	144	7	the	the	DT
cord-021063-4y8m33ea	144	8	calcium	calcium	NN
cord-021063-4y8m33ea	144	9	,	,	,
cord-021063-4y8m33ea	144	10	these	these	DT
cord-021063-4y8m33ea	144	11	structures	structure	NNS
cord-021063-4y8m33ea	144	12	form	form	VBP
cord-021063-4y8m33ea	144	13	large	large	JJ
cord-021063-4y8m33ea	144	14	unilamellar	unilamellar	JJ
cord-021063-4y8m33ea	144	15	vesicles	vesicle	NNS
cord-021063-4y8m33ea	144	16	(	(	-LRB-
cord-021063-4y8m33ea	144	17	LUVs	LUVs	NNP
cord-021063-4y8m33ea	144	18	)	)	-RRB-
cord-021063-4y8m33ea	145	1	(	(	-LRB-
cord-021063-4y8m33ea	145	2	Papahadjopoulos	Papahadjopoulos	NNP
cord-021063-4y8m33ea	145	3	et	et	NNP
cord-021063-4y8m33ea	145	4	al	al	NNP
cord-021063-4y8m33ea	145	5	.	.	NNP
cord-021063-4y8m33ea	145	6	,	,	,
cord-021063-4y8m33ea	145	7	1975	1975	CD
cord-021063-4y8m33ea	145	8	)	)	-RRB-
cord-021063-4y8m33ea	145	9	.	.	.
cord-021063-4y8m33ea	146	1	In	in	IN
cord-021063-4y8m33ea	146	2	the	the	DT
cord-021063-4y8m33ea	146	3	process	process	NN
cord-021063-4y8m33ea	146	4	,	,	,
cord-021063-4y8m33ea	146	5	the	the	DT
cord-021063-4y8m33ea	146	6	vesicles	vesicle	NNS
cord-021063-4y8m33ea	146	7	encapsulate	encapsulate	VBP
cord-021063-4y8m33ea	146	8	a	a	DT
cord-021063-4y8m33ea	146	9	substantial	substantial	JJ
cord-021063-4y8m33ea	146	10	fraction	fraction	NN
cord-021063-4y8m33ea	146	11	of	of	IN
cord-021063-4y8m33ea	146	12	the	the	DT
cord-021063-4y8m33ea	146	13	surrounding	surround	VBG
cord-021063-4y8m33ea	146	14	solvent	solvent	NN
cord-021063-4y8m33ea	146	15	and	and	CC
cord-021063-4y8m33ea	146	16	solutes	solute	NNS
cord-021063-4y8m33ea	146	17	.	.	.
cord-021063-4y8m33ea	147	1	If	if	IN
cord-021063-4y8m33ea	147	2	DNA	DNA	NNP
cord-021063-4y8m33ea	147	3	is	be	VBZ
cord-021063-4y8m33ea	147	4	present	present	JJ
cord-021063-4y8m33ea	147	5	,	,	,
cord-021063-4y8m33ea	147	6	some	some	DT
cord-021063-4y8m33ea	147	7	of	of	IN
cord-021063-4y8m33ea	147	8	it	-PRON-	PRP
cord-021063-4y8m33ea	147	9	becomes	become	VBZ
cord-021063-4y8m33ea	147	10	encapsulated	encapsulate	VBN
cord-021063-4y8m33ea	147	11	in	in	IN
cord-021063-4y8m33ea	147	12	the	the	DT
cord-021063-4y8m33ea	147	13	vesicles	vesicle	NNS
cord-021063-4y8m33ea	147	14	.	.	.
cord-021063-4y8m33ea	148	1	Two	two	CD
cord-021063-4y8m33ea	148	2	advantages	advantage	NNS
cord-021063-4y8m33ea	148	3	of	of	IN
cord-021063-4y8m33ea	148	4	this	this	DT
cord-021063-4y8m33ea	148	5	technique	technique	NN
cord-021063-4y8m33ea	148	6	are	be	VBP
cord-021063-4y8m33ea	148	7	:	:	:
cord-021063-4y8m33ea	148	8	(	(	-LRB-
cord-021063-4y8m33ea	148	9	i	i	NN
cord-021063-4y8m33ea	148	10	)	)	-RRB-
cord-021063-4y8m33ea	149	1	the	the	DT
cord-021063-4y8m33ea	149	2	DNA	DNA	NNP
cord-021063-4y8m33ea	149	3	is	be	VBZ
cord-021063-4y8m33ea	149	4	not	not	RB
cord-021063-4y8m33ea	149	5	exposed	expose	VBN
cord-021063-4y8m33ea	149	6	to	to	IN
cord-021063-4y8m33ea	149	7	harsh	harsh	JJ
cord-021063-4y8m33ea	149	8	conditions	condition	NNS
cord-021063-4y8m33ea	149	9	that	that	WDT
cord-021063-4y8m33ea	149	10	would	would	MD
cord-021063-4y8m33ea	149	11	degrade	degrade	VB
cord-021063-4y8m33ea	149	12	it	-PRON-	PRP
cord-021063-4y8m33ea	149	13	,	,	,
cord-021063-4y8m33ea	149	14	and	and	CC
cord-021063-4y8m33ea	149	15	(	(	-LRB-
cord-021063-4y8m33ea	149	16	ii	ii	NN
cord-021063-4y8m33ea	149	17	)	)	-RRB-
cord-021063-4y8m33ea	150	1	the	the	DT
cord-021063-4y8m33ea	150	2	entrapment	entrapment	NN
cord-021063-4y8m33ea	150	3	is	be	VBZ
cord-021063-4y8m33ea	150	4	relatively	relatively	RB
cord-021063-4y8m33ea	150	5	efficient	efficient	JJ
cord-021063-4y8m33ea	150	6	.	.	.
cord-021063-4y8m33ea	151	1	However	however	RB
cord-021063-4y8m33ea	151	2	,	,	,
cord-021063-4y8m33ea	151	3	the	the	DT
cord-021063-4y8m33ea	151	4	requirement	requirement	NN
cord-021063-4y8m33ea	151	5	for	for	IN
cord-021063-4y8m33ea	151	6	phosphatidylserine	phosphatidylserine	NN
cord-021063-4y8m33ea	151	7	makes	make	VBZ
cord-021063-4y8m33ea	151	8	this	this	DT
cord-021063-4y8m33ea	151	9	technique	technique	NN
cord-021063-4y8m33ea	151	10	impractical	impractical	JJ
cord-021063-4y8m33ea	151	11	for	for	IN
cord-021063-4y8m33ea	151	12	most	most	JJS
cord-021063-4y8m33ea	151	13	in	in	IN
cord-021063-4y8m33ea	151	14	vivo	vivo	NN
cord-021063-4y8m33ea	151	15	applications	application	NNS
cord-021063-4y8m33ea	151	16	,	,	,
cord-021063-4y8m33ea	151	17	with	with	IN
cord-021063-4y8m33ea	151	18	the	the	DT
cord-021063-4y8m33ea	151	19	exception	exception	NN
cord-021063-4y8m33ea	151	20	of	of	IN
cord-021063-4y8m33ea	151	21	transfecting	transfecting	NN
cord-021063-4y8m33ea	151	22	macrophages	macrophage	NNS
cord-021063-4y8m33ea	151	23	which	which	WDT
cord-021063-4y8m33ea	151	24	have	have	VBP
cord-021063-4y8m33ea	151	25	cell	cell	NN
cord-021063-4y8m33ea	151	26	surface	surface	NN
cord-021063-4y8m33ea	151	27	receptors	receptor	NNS
cord-021063-4y8m33ea	151	28	for	for	IN
cord-021063-4y8m33ea	151	29	phosphatidylserine	phosphatidylserine	NN
cord-021063-4y8m33ea	151	30	(	(	-LRB-
cord-021063-4y8m33ea	151	31	PS	PS	NNP
cord-021063-4y8m33ea	151	32	)	)	-RRB-
cord-021063-4y8m33ea	151	33	.	.	.
cord-021063-4y8m33ea	152	1	Detergent	Detergent	NNP
cord-021063-4y8m33ea	152	2	Dialysis	Dialysis	NNP
cord-021063-4y8m33ea	152	3	(	(	-LRB-
cord-021063-4y8m33ea	152	4	and	and	CC
cord-021063-4y8m33ea	152	5	Virosomes	Virosomes	NNP
cord-021063-4y8m33ea	152	6	)	)	-RRB-
cord-021063-4y8m33ea	152	7	.	.	.
cord-021063-4y8m33ea	153	1	When	when	WRB
cord-021063-4y8m33ea	153	2	membranes	membrane	NNS
cord-021063-4y8m33ea	153	3	are	be	VBP
cord-021063-4y8m33ea	153	4	mixed	mix	VBN
cord-021063-4y8m33ea	153	5	with	with	IN
cord-021063-4y8m33ea	153	6	detergents	detergent	NNS
cord-021063-4y8m33ea	153	7	at	at	IN
cord-021063-4y8m33ea	153	8	concentrations	concentration	NNS
cord-021063-4y8m33ea	153	9	above	above	IN
cord-021063-4y8m33ea	153	10	their	-PRON-	PRP$
cord-021063-4y8m33ea	153	11	critical	critical	JJ
cord-021063-4y8m33ea	153	12	mixed	mixed	JJ
cord-021063-4y8m33ea	153	13	micellar	micellar	JJ
cord-021063-4y8m33ea	153	14	concentration	concentration	NN
cord-021063-4y8m33ea	153	15	(	(	-LRB-
cord-021063-4y8m33ea	153	16	CMMC	CMMC	NNP
cord-021063-4y8m33ea	153	17	)	)	-RRB-
cord-021063-4y8m33ea	153	18	,	,	,
cord-021063-4y8m33ea	153	19	they	-PRON-	PRP
cord-021063-4y8m33ea	153	20	combine	combine	VBP
cord-021063-4y8m33ea	153	21	with	with	IN
cord-021063-4y8m33ea	153	22	the	the	DT
cord-021063-4y8m33ea	153	23	membrane	membrane	NN
cord-021063-4y8m33ea	153	24	's	's	POS
cord-021063-4y8m33ea	153	25	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	153	26	and	and	CC
cord-021063-4y8m33ea	153	27	proteins	protein	NNS
cord-021063-4y8m33ea	153	28	to	to	TO
cord-021063-4y8m33ea	153	29	form	form	VB
cord-021063-4y8m33ea	153	30	mixed	mixed	JJ
cord-021063-4y8m33ea	153	31	micelles	micelle	NNS
cord-021063-4y8m33ea	153	32	.	.	.
cord-021063-4y8m33ea	154	1	Because	because	IN
cord-021063-4y8m33ea	154	2	detergents	detergent	NNS
cord-021063-4y8m33ea	154	3	are	be	VBP
cord-021063-4y8m33ea	154	4	relatively	relatively	RB
cord-021063-4y8m33ea	154	5	water	water	NN
cord-021063-4y8m33ea	154	6	soluble	soluble	JJ
cord-021063-4y8m33ea	154	7	,	,	,
cord-021063-4y8m33ea	154	8	they	-PRON-	PRP
cord-021063-4y8m33ea	154	9	can	can	MD
cord-021063-4y8m33ea	154	10	be	be	VB
cord-021063-4y8m33ea	154	11	removed	remove	VBN
cord-021063-4y8m33ea	154	12	from	from	IN
cord-021063-4y8m33ea	154	13	the	the	DT
cord-021063-4y8m33ea	154	14	mixed	mixed	JJ
cord-021063-4y8m33ea	154	15	micelles	micelle	NNS
cord-021063-4y8m33ea	154	16	by	by	IN
cord-021063-4y8m33ea	154	17	dialysis	dialysis	NN
cord-021063-4y8m33ea	154	18	.	.	.
cord-021063-4y8m33ea	155	1	When	when	WRB
cord-021063-4y8m33ea	155	2	their	-PRON-	PRP$
cord-021063-4y8m33ea	155	3	concentration	concentration	NN
cord-021063-4y8m33ea	155	4	in	in	IN
cord-021063-4y8m33ea	155	5	the	the	DT
cord-021063-4y8m33ea	155	6	micelles	micelle	NNS
cord-021063-4y8m33ea	155	7	falls	fall	VBZ
cord-021063-4y8m33ea	155	8	below	below	IN
cord-021063-4y8m33ea	155	9	the	the	DT
cord-021063-4y8m33ea	155	10	CMMC	CMMC	NNP
cord-021063-4y8m33ea	155	11	,	,	,
cord-021063-4y8m33ea	155	12	membrane	membrane	NN
cord-021063-4y8m33ea	155	13	bilayers	bilayer	NNS
cord-021063-4y8m33ea	155	14	regenerate	regenerate	VBP
cord-021063-4y8m33ea	155	15	and	and	CC
cord-021063-4y8m33ea	155	16	form	form	NN
cord-021063-4y8m33ea	155	17	liposomes	liposome	NNS
cord-021063-4y8m33ea	155	18	.	.	.
cord-021063-4y8m33ea	156	1	Enveloped	envelop	VBN
cord-021063-4y8m33ea	156	2	viruses	virus	NNS
cord-021063-4y8m33ea	156	3	have	have	VBP
cord-021063-4y8m33ea	156	4	membrane	membrane	NN
cord-021063-4y8m33ea	156	5	proteins	protein	NNS
cord-021063-4y8m33ea	156	6	that	that	WDT
cord-021063-4y8m33ea	156	7	promote	promote	VBP
cord-021063-4y8m33ea	156	8	the	the	DT
cord-021063-4y8m33ea	156	9	fusion	fusion	NN
cord-021063-4y8m33ea	156	10	of	of	IN
cord-021063-4y8m33ea	156	11	the	the	DT
cord-021063-4y8m33ea	156	12	viral	viral	JJ
cord-021063-4y8m33ea	156	13	membrane	membrane	NN
cord-021063-4y8m33ea	156	14	with	with	IN
cord-021063-4y8m33ea	156	15	cellular	cellular	JJ
cord-021063-4y8m33ea	156	16	membranes	membrane	NNS
cord-021063-4y8m33ea	156	17	.	.	.
cord-021063-4y8m33ea	157	1	When	when	WRB
cord-021063-4y8m33ea	157	2	an	an	DT
cord-021063-4y8m33ea	157	3	enveloped	envelop	VBN
cord-021063-4y8m33ea	157	4	virus	virus	NN
cord-021063-4y8m33ea	157	5	fuses	fuse	VBZ
cord-021063-4y8m33ea	157	6	to	to	IN
cord-021063-4y8m33ea	157	7	a	a	DT
cord-021063-4y8m33ea	157	8	cell	cell	NN
cord-021063-4y8m33ea	157	9	,	,	,
cord-021063-4y8m33ea	157	10	the	the	DT
cord-021063-4y8m33ea	157	11	nucleocapsid	nucleocapsid	NN
cord-021063-4y8m33ea	157	12	is	be	VBZ
cord-021063-4y8m33ea	157	13	released	release	VBN
cord-021063-4y8m33ea	157	14	into	into	IN
cord-021063-4y8m33ea	157	15	the	the	DT
cord-021063-4y8m33ea	157	16	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	157	17	,	,	,
cord-021063-4y8m33ea	157	18	and	and	CC
cord-021063-4y8m33ea	157	19	the	the	DT
cord-021063-4y8m33ea	157	20	infection	infection	NN
cord-021063-4y8m33ea	157	21	proceeds	proceed	VBZ
cord-021063-4y8m33ea	157	22	.	.	.
cord-021063-4y8m33ea	158	1	Detergent	detergent	NN
cord-021063-4y8m33ea	158	2	solubilization	solubilization	NN
cord-021063-4y8m33ea	158	3	of	of	IN
cord-021063-4y8m33ea	158	4	enveloped	enveloped	JJ
cord-021063-4y8m33ea	158	5	viruses	virus	NNS
cord-021063-4y8m33ea	158	6	produces	produce	VBZ
cord-021063-4y8m33ea	158	7	a	a	DT
cord-021063-4y8m33ea	158	8	micellar	micellar	JJ
cord-021063-4y8m33ea	158	9	mixture	mixture	NN
cord-021063-4y8m33ea	158	10	containing	contain	VBG
cord-021063-4y8m33ea	158	11	detergent	detergent	NN
cord-021063-4y8m33ea	158	12	,	,	,
cord-021063-4y8m33ea	158	13	viral	viral	JJ
cord-021063-4y8m33ea	158	14	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	158	15	,	,	,
cord-021063-4y8m33ea	158	16	and	and	CC
cord-021063-4y8m33ea	158	17	viral	viral	JJ
cord-021063-4y8m33ea	158	18	membrane	membrane	NN
cord-021063-4y8m33ea	158	19	proteins	protein	NNS
cord-021063-4y8m33ea	158	20	.	.	.
cord-021063-4y8m33ea	159	1	Dialysis	dialysis	NN
cord-021063-4y8m33ea	159	2	of	of	IN
cord-021063-4y8m33ea	159	3	these	these	DT
cord-021063-4y8m33ea	159	4	detergent	detergent	NN
cord-021063-4y8m33ea	159	5	mixtures	mixture	NNS
cord-021063-4y8m33ea	159	6	results	result	NNS
cord-021063-4y8m33ea	159	7	in	in	IN
cord-021063-4y8m33ea	159	8	the	the	DT
cord-021063-4y8m33ea	159	9	production	production	NN
cord-021063-4y8m33ea	159	10	of	of	IN
cord-021063-4y8m33ea	159	11	liposomes	liposome	NNS
cord-021063-4y8m33ea	159	12	that	that	WDT
cord-021063-4y8m33ea	159	13	are	be	VBP
cord-021063-4y8m33ea	159	14	often	often	RB
cord-021063-4y8m33ea	159	15	called	call	VBN
cord-021063-4y8m33ea	159	16	virosomes	virosome	NNS
cord-021063-4y8m33ea	159	17	to	to	TO
cord-021063-4y8m33ea	159	18	indicate	indicate	VB
cord-021063-4y8m33ea	159	19	the	the	DT
cord-021063-4y8m33ea	159	20	presence	presence	NN
cord-021063-4y8m33ea	159	21	of	of	IN
cord-021063-4y8m33ea	159	22	viral	viral	JJ
cord-021063-4y8m33ea	159	23	proteins	protein	NNS
cord-021063-4y8m33ea	159	24	.	.	.
cord-021063-4y8m33ea	160	1	If	if	IN
cord-021063-4y8m33ea	160	2	the	the	DT
cord-021063-4y8m33ea	160	3	dialysis	dialysis	NN
cord-021063-4y8m33ea	160	4	is	be	VBZ
cord-021063-4y8m33ea	160	5	performed	perform	VBN
cord-021063-4y8m33ea	160	6	properly	properly	RB
cord-021063-4y8m33ea	160	7	,	,	,
cord-021063-4y8m33ea	160	8	the	the	DT
cord-021063-4y8m33ea	160	9	fusion	fusion	NN
cord-021063-4y8m33ea	160	10	proteins	protein	NNS
cord-021063-4y8m33ea	160	11	of	of	IN
cord-021063-4y8m33ea	160	12	many	many	JJ
cord-021063-4y8m33ea	160	13	viruses	virus	NNS
cord-021063-4y8m33ea	160	14	can	can	MD
cord-021063-4y8m33ea	160	15	be	be	VB
cord-021063-4y8m33ea	160	16	reconstituted	reconstitute	VBN
cord-021063-4y8m33ea	160	17	in	in	IN
cord-021063-4y8m33ea	160	18	an	an	DT
cord-021063-4y8m33ea	160	19	active	active	JJ
cord-021063-4y8m33ea	160	20	conformation	conformation	NN
cord-021063-4y8m33ea	160	21	(	(	-LRB-
cord-021063-4y8m33ea	160	22	White	White	NNP
cord-021063-4y8m33ea	160	23	et	et	FW
cord-021063-4y8m33ea	160	24	al	al	NNP
cord-021063-4y8m33ea	160	25	.	.	NNP
cord-021063-4y8m33ea	160	26	,	,	,
cord-021063-4y8m33ea	160	27	1983	1983	CD
cord-021063-4y8m33ea	160	28	)	)	-RRB-
cord-021063-4y8m33ea	160	29	.	.	.
cord-021063-4y8m33ea	161	1	Viral	viral	JJ
cord-021063-4y8m33ea	161	2	fusion	fusion	NN
cord-021063-4y8m33ea	161	3	proteins	protein	NNS
cord-021063-4y8m33ea	161	4	having	have	VBG
cord-021063-4y8m33ea	161	5	a	a	DT
cord-021063-4y8m33ea	161	6	variety	variety	NN
cord-021063-4y8m33ea	161	7	of	of	IN
cord-021063-4y8m33ea	161	8	different	different	JJ
cord-021063-4y8m33ea	161	9	characteristics	characteristic	NNS
cord-021063-4y8m33ea	161	10	have	have	VBP
cord-021063-4y8m33ea	161	11	been	be	VBN
cord-021063-4y8m33ea	161	12	identified	identify	VBN
cord-021063-4y8m33ea	161	13	.	.	.
cord-021063-4y8m33ea	162	1	For	for	IN
cord-021063-4y8m33ea	162	2	example	example	NN
cord-021063-4y8m33ea	162	3	,	,	,
cord-021063-4y8m33ea	162	4	some	some	DT
cord-021063-4y8m33ea	162	5	are	be	VBP
cord-021063-4y8m33ea	162	6	only	only	RB
cord-021063-4y8m33ea	162	7	active	active	JJ
cord-021063-4y8m33ea	162	8	at	at	IN
cord-021063-4y8m33ea	162	9	low	low	JJ
cord-021063-4y8m33ea	162	10	pH	ph	NN
cord-021063-4y8m33ea	162	11	and	and	CC
cord-021063-4y8m33ea	162	12	fuse	fuse	VB
cord-021063-4y8m33ea	162	13	with	with	IN
cord-021063-4y8m33ea	162	14	endosomal	endosomal	NNP
cord-021063-4y8m33ea	162	15	membranes	membrane	NNS
cord-021063-4y8m33ea	162	16	,	,	,
cord-021063-4y8m33ea	162	17	while	while	IN
cord-021063-4y8m33ea	162	18	others	other	NNS
cord-021063-4y8m33ea	162	19	are	be	VBP
cord-021063-4y8m33ea	162	20	active	active	JJ
cord-021063-4y8m33ea	162	21	at	at	IN
cord-021063-4y8m33ea	162	22	physiological	physiological	JJ
cord-021063-4y8m33ea	162	23	pH	ph	NN
cord-021063-4y8m33ea	162	24	and	and	CC
cord-021063-4y8m33ea	162	25	fuse	fuse	VB
cord-021063-4y8m33ea	162	26	to	to	IN
cord-021063-4y8m33ea	162	27	the	the	DT
cord-021063-4y8m33ea	162	28	plasma	plasma	NN
cord-021063-4y8m33ea	162	29	membrane	membrane	NN
cord-021063-4y8m33ea	162	30	.	.	.
cord-021063-4y8m33ea	163	1	Some	some	DT
cord-021063-4y8m33ea	163	2	viral	viral	JJ
cord-021063-4y8m33ea	163	3	fusion	fusion	NN
cord-021063-4y8m33ea	163	4	proteins	protein	NNS
cord-021063-4y8m33ea	163	5	,	,	,
cord-021063-4y8m33ea	163	6	such	such	JJ
cord-021063-4y8m33ea	163	7	as	as	IN
cord-021063-4y8m33ea	163	8	the	the	DT
cord-021063-4y8m33ea	163	9	HA	HA	NNP
cord-021063-4y8m33ea	163	10	protein	protein	NN
cord-021063-4y8m33ea	163	11	of	of	IN
cord-021063-4y8m33ea	163	12	influenza	influenza	NN
cord-021063-4y8m33ea	163	13	,	,	,
cord-021063-4y8m33ea	163	14	bind	bind	VB
cord-021063-4y8m33ea	163	15	to	to	IN
cord-021063-4y8m33ea	163	16	specific	specific	JJ
cord-021063-4y8m33ea	163	17	receptors	receptor	NNS
cord-021063-4y8m33ea	163	18	before	before	IN
cord-021063-4y8m33ea	163	19	fusion	fusion	NN
cord-021063-4y8m33ea	163	20	.	.	.
cord-021063-4y8m33ea	164	1	A	a	DT
cord-021063-4y8m33ea	164	2	few	few	JJ
cord-021063-4y8m33ea	164	3	viral	viral	JJ
cord-021063-4y8m33ea	164	4	fusion	fusion	NN
cord-021063-4y8m33ea	164	5	proteins	protein	NNS
cord-021063-4y8m33ea	164	6	will	will	MD
cord-021063-4y8m33ea	164	7	only	only	RB
cord-021063-4y8m33ea	164	8	promote	promote	VB
cord-021063-4y8m33ea	164	9	fusion	fusion	NN
cord-021063-4y8m33ea	164	10	to	to	TO
cord-021063-4y8m33ea	164	11	target	target	VB
cord-021063-4y8m33ea	164	12	membranes	membrane	NNS
cord-021063-4y8m33ea	164	13	containing	contain	VBG
cord-021063-4y8m33ea	164	14	a	a	DT
cord-021063-4y8m33ea	164	15	specific	specific	JJ
cord-021063-4y8m33ea	164	16	mixture	mixture	NN
cord-021063-4y8m33ea	164	17	of	of	IN
cord-021063-4y8m33ea	164	18	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	164	19	(	(	-LRB-
cord-021063-4y8m33ea	164	20	Cervin	Cervin	NNP
cord-021063-4y8m33ea	164	21	and	and	CC
cord-021063-4y8m33ea	164	22	Anderson	Anderson	NNP
cord-021063-4y8m33ea	164	23	,	,	,
cord-021063-4y8m33ea	164	24	1991	1991	CD
cord-021063-4y8m33ea	164	25	;	;	:
cord-021063-4y8m33ea	164	26	Nussbaum	Nussbaum	NNP
cord-021063-4y8m33ea	164	27	et	et	NNP
cord-021063-4y8m33ea	164	28	al	al	NNP
cord-021063-4y8m33ea	164	29	.	.	NNP
cord-021063-4y8m33ea	164	30	,	,	,
cord-021063-4y8m33ea	164	31	1992	1992	CD
cord-021063-4y8m33ea	164	32	)	)	-RRB-
cord-021063-4y8m33ea	164	33	.	.	.
cord-021063-4y8m33ea	165	1	The	the	DT
cord-021063-4y8m33ea	165	2	major	major	JJ
cord-021063-4y8m33ea	165	3	advantage	advantage	NN
cord-021063-4y8m33ea	165	4	of	of	IN
cord-021063-4y8m33ea	165	5	detergent	detergent	NN
cord-021063-4y8m33ea	165	6	dialysis	dialysis	NN
cord-021063-4y8m33ea	165	7	is	be	VBZ
cord-021063-4y8m33ea	165	8	that	that	IN
cord-021063-4y8m33ea	165	9	it	-PRON-	PRP
cord-021063-4y8m33ea	165	10	is	be	VBZ
cord-021063-4y8m33ea	165	11	very	very	RB
cord-021063-4y8m33ea	165	12	gentle	gentle	JJ
cord-021063-4y8m33ea	165	13	since	since	IN
cord-021063-4y8m33ea	165	14	no	no	DT
cord-021063-4y8m33ea	165	15	sonication	sonication	NN
cord-021063-4y8m33ea	165	16	,	,	,
cord-021063-4y8m33ea	165	17	vortexing	vortexing	NN
cord-021063-4y8m33ea	165	18	,	,	,
cord-021063-4y8m33ea	165	19	or	or	CC
cord-021063-4y8m33ea	165	20	organic	organic	JJ
cord-021063-4y8m33ea	165	21	solvent	solvent	NN
cord-021063-4y8m33ea	165	22	is	be	VBZ
cord-021063-4y8m33ea	165	23	used	use	VBN
cord-021063-4y8m33ea	165	24	.	.	.
cord-021063-4y8m33ea	166	1	This	this	DT
cord-021063-4y8m33ea	166	2	results	result	VBZ
cord-021063-4y8m33ea	166	3	in	in	IN
cord-021063-4y8m33ea	166	4	only	only	RB
cord-021063-4y8m33ea	166	5	a	a	DT
cord-021063-4y8m33ea	166	6	small	small	JJ
cord-021063-4y8m33ea	166	7	amount	amount	NN
cord-021063-4y8m33ea	166	8	of	of	IN
cord-021063-4y8m33ea	166	9	DNA	dna	NN
cord-021063-4y8m33ea	166	10	fragmentation	fragmentation	NN
cord-021063-4y8m33ea	166	11	during	during	IN
cord-021063-4y8m33ea	166	12	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	166	13	.	.	.
cord-021063-4y8m33ea	167	1	Unfortunately	unfortunately	RB
cord-021063-4y8m33ea	167	2	,	,	,
cord-021063-4y8m33ea	167	3	the	the	DT
cord-021063-4y8m33ea	167	4	efficiency	efficiency	NN
cord-021063-4y8m33ea	167	5	of	of	IN
cord-021063-4y8m33ea	167	6	entrapment	entrapment	NN
cord-021063-4y8m33ea	167	7	is	be	VBZ
cord-021063-4y8m33ea	167	8	very	very	RB
cord-021063-4y8m33ea	167	9	low	low	JJ
cord-021063-4y8m33ea	167	10	,	,	,
cord-021063-4y8m33ea	167	11	causing	cause	VBG
cord-021063-4y8m33ea	167	12	much	much	JJ
cord-021063-4y8m33ea	167	13	of	of	IN
cord-021063-4y8m33ea	167	14	the	the	DT
cord-021063-4y8m33ea	167	15	DNA	dna	NN
cord-021063-4y8m33ea	167	16	to	to	TO
cord-021063-4y8m33ea	167	17	be	be	VB
cord-021063-4y8m33ea	167	18	wasted	waste	VBN
cord-021063-4y8m33ea	167	19	and	and	CC
cord-021063-4y8m33ea	167	20	resulting	result	VBG
cord-021063-4y8m33ea	167	21	in	in	IN
cord-021063-4y8m33ea	167	22	a	a	DT
cord-021063-4y8m33ea	167	23	high	high	JJ
cord-021063-4y8m33ea	167	24	fraction	fraction	NN
cord-021063-4y8m33ea	167	25	of	of	IN
cord-021063-4y8m33ea	167	26	empty	empty	JJ
cord-021063-4y8m33ea	167	27	liposomes	liposome	NNS
cord-021063-4y8m33ea	167	28	being	be	VBG
cord-021063-4y8m33ea	167	29	formed	form	VBN
cord-021063-4y8m33ea	167	30	.	.	.
cord-021063-4y8m33ea	168	1	Loyter	Loyter	NNP
cord-021063-4y8m33ea	168	2	and	and	CC
cord-021063-4y8m33ea	168	3	co	co	NNS
cord-021063-4y8m33ea	168	4	-	-	NNS
cord-021063-4y8m33ea	168	5	workers	worker	NNS
cord-021063-4y8m33ea	168	6	have	have	VBP
cord-021063-4y8m33ea	168	7	fused	fuse	VBN
cord-021063-4y8m33ea	168	8	DNA	dna	NN
cord-021063-4y8m33ea	168	9	-	-	HYPH
cord-021063-4y8m33ea	168	10	containing	containing	NN
cord-021063-4y8m33ea	168	11	liposomes	liposome	NNS
cord-021063-4y8m33ea	168	12	with	with	IN
cord-021063-4y8m33ea	168	13	cells	cell	NNS
cord-021063-4y8m33ea	168	14	by	by	IN
cord-021063-4y8m33ea	168	15	making	make	VBG
cord-021063-4y8m33ea	168	16	empty	empty	JJ
cord-021063-4y8m33ea	168	17	virosomes	virosome	NNS
cord-021063-4y8m33ea	168	18	and	and	CC
cord-021063-4y8m33ea	168	19	DNA	dna	NN
cord-021063-4y8m33ea	168	20	-	-	HYPH
cord-021063-4y8m33ea	168	21	containing	containing	NN
cord-021063-4y8m33ea	168	22	liposomes	liposome	NNS
cord-021063-4y8m33ea	168	23	separately	separately	RB
cord-021063-4y8m33ea	168	24	,	,	,
cord-021063-4y8m33ea	168	25	and	and	CC
cord-021063-4y8m33ea	168	26	then	then	RB
cord-021063-4y8m33ea	168	27	fusing	fuse	VBG
cord-021063-4y8m33ea	168	28	the	the	DT
cord-021063-4y8m33ea	168	29	two	two	CD
cord-021063-4y8m33ea	168	30	populations	population	NNS
cord-021063-4y8m33ea	168	31	of	of	IN
cord-021063-4y8m33ea	168	32	vesicles	vesicle	NNS
cord-021063-4y8m33ea	168	33	in	in	IN
cord-021063-4y8m33ea	168	34	the	the	DT
cord-021063-4y8m33ea	168	35	presence	presence	NN
cord-021063-4y8m33ea	168	36	of	of	IN
cord-021063-4y8m33ea	168	37	target	target	NN
cord-021063-4y8m33ea	168	38	cells	cell	NNS
cord-021063-4y8m33ea	168	39	(	(	-LRB-
cord-021063-4y8m33ea	168	40	Lapidot	Lapidot	NNP
cord-021063-4y8m33ea	168	41	and	and	CC
cord-021063-4y8m33ea	168	42	Loyter	Loyter	NNP
cord-021063-4y8m33ea	168	43	,	,	,
cord-021063-4y8m33ea	168	44	1990	1990	CD
cord-021063-4y8m33ea	168	45	)	)	-RRB-
cord-021063-4y8m33ea	168	46	.	.	.
cord-021063-4y8m33ea	169	1	To	to	IN
cord-021063-4y8m33ea	169	2	date	date	NN
cord-021063-4y8m33ea	169	3	,	,	,
cord-021063-4y8m33ea	169	4	this	this	DT
cord-021063-4y8m33ea	169	5	technique	technique	NN
cord-021063-4y8m33ea	169	6	has	have	VBZ
cord-021063-4y8m33ea	169	7	not	not	RB
cord-021063-4y8m33ea	169	8	been	be	VBN
cord-021063-4y8m33ea	169	9	used	use	VBN
cord-021063-4y8m33ea	169	10	in	in	IN
cord-021063-4y8m33ea	169	11	vivo	vivo	NN
cord-021063-4y8m33ea	169	12	.	.	.
cord-021063-4y8m33ea	170	1	Reverse	Reverse	NNP
cord-021063-4y8m33ea	170	2	-	-	HYPH
cord-021063-4y8m33ea	170	3	Phase	phase	NN
cord-021063-4y8m33ea	170	4	Evaporation	evaporation	NN
cord-021063-4y8m33ea	170	5	.	.	.
cord-021063-4y8m33ea	171	1	Currently	currently	RB
cord-021063-4y8m33ea	171	2	,	,	,
cord-021063-4y8m33ea	171	3	the	the	DT
cord-021063-4y8m33ea	171	4	highest	high	JJS
cord-021063-4y8m33ea	171	5	DNA	dna	NN
cord-021063-4y8m33ea	171	6	entrapment	entrapment	NN
cord-021063-4y8m33ea	171	7	efficiencies	efficiency	NNS
cord-021063-4y8m33ea	171	8	can	can	MD
cord-021063-4y8m33ea	171	9	be	be	VB
cord-021063-4y8m33ea	171	10	achieved	achieve	VBN
cord-021063-4y8m33ea	171	11	when	when	WRB
cord-021063-4y8m33ea	171	12	vesicles	vesicle	NNS
cord-021063-4y8m33ea	171	13	(	(	-LRB-
cord-021063-4y8m33ea	171	14	REVs	rev	NNS
cord-021063-4y8m33ea	171	15	)	)	-RRB-
cord-021063-4y8m33ea	171	16	are	be	VBP
cord-021063-4y8m33ea	171	17	prepared	prepare	VBN
cord-021063-4y8m33ea	171	18	by	by	IN
cord-021063-4y8m33ea	171	19	reverse	reverse	JJ
cord-021063-4y8m33ea	171	20	-	-	HYPH
cord-021063-4y8m33ea	171	21	phase	phase	NN
cord-021063-4y8m33ea	171	22	evaporation	evaporation	NN
cord-021063-4y8m33ea	172	1	(	(	-LRB-
cord-021063-4y8m33ea	172	2	Fraley	Fraley	NNP
cord-021063-4y8m33ea	172	3	et	et	NNP
cord-021063-4y8m33ea	172	4	al	al	NNP
cord-021063-4y8m33ea	172	5	.	.	NNP
cord-021063-4y8m33ea	172	6	,	,	,
cord-021063-4y8m33ea	172	7	1980	1980	CD
cord-021063-4y8m33ea	172	8	)	)	-RRB-
cord-021063-4y8m33ea	172	9	.	.	.
cord-021063-4y8m33ea	173	1	REVs	rev	NNS
cord-021063-4y8m33ea	173	2	are	be	VBP
cord-021063-4y8m33ea	173	3	prepared	prepare	VBN
cord-021063-4y8m33ea	173	4	by	by	IN
cord-021063-4y8m33ea	173	5	bath	bath	NN
cord-021063-4y8m33ea	173	6	sonication	sonication	NN
cord-021063-4y8m33ea	173	7	of	of	IN
cord-021063-4y8m33ea	173	8	a	a	DT
cord-021063-4y8m33ea	173	9	mixture	mixture	NN
cord-021063-4y8m33ea	173	10	of	of	IN
cord-021063-4y8m33ea	173	11	lipid	lipid	NN
cord-021063-4y8m33ea	173	12	,	,	,
cord-021063-4y8m33ea	173	13	DNA	dna	NN
cord-021063-4y8m33ea	173	14	,	,	,
cord-021063-4y8m33ea	173	15	ether	ether	NN
cord-021063-4y8m33ea	173	16	,	,	,
cord-021063-4y8m33ea	173	17	and	and	CC
cord-021063-4y8m33ea	173	18	buffered	buffer	VBN
cord-021063-4y8m33ea	173	19	saline	saline	NN
cord-021063-4y8m33ea	173	20	,	,	,
cord-021063-4y8m33ea	173	21	followed	follow	VBN
cord-021063-4y8m33ea	173	22	by	by	IN
cord-021063-4y8m33ea	173	23	evaporation	evaporation	NN
cord-021063-4y8m33ea	173	24	of	of	IN
cord-021063-4y8m33ea	173	25	the	the	DT
cord-021063-4y8m33ea	173	26	ether	ether	NN
cord-021063-4y8m33ea	173	27	to	to	TO
cord-021063-4y8m33ea	173	28	form	form	VB
cord-021063-4y8m33ea	173	29	a	a	DT
cord-021063-4y8m33ea	173	30	paste	paste	NN
cord-021063-4y8m33ea	173	31	of	of	IN
cord-021063-4y8m33ea	173	32	phospholipid	phospholipid	NN
cord-021063-4y8m33ea	173	33	inverted	invert	VBN
cord-021063-4y8m33ea	173	34	monolayers	monolayer	NNS
cord-021063-4y8m33ea	173	35	surrounding	surround	VBG
cord-021063-4y8m33ea	173	36	aqueous	aqueous	JJ
cord-021063-4y8m33ea	173	37	droplets	droplet	NNS
cord-021063-4y8m33ea	173	38	.	.	.
cord-021063-4y8m33ea	174	1	These	these	DT
cord-021063-4y8m33ea	174	2	are	be	VBP
cord-021063-4y8m33ea	174	3	resolved	resolve	VBN
cord-021063-4y8m33ea	174	4	into	into	IN
cord-021063-4y8m33ea	174	5	liposomes	liposome	NNS
cord-021063-4y8m33ea	174	6	by	by	IN
cord-021063-4y8m33ea	174	7	vortexing	vortexe	VBG
cord-021063-4y8m33ea	174	8	the	the	DT
cord-021063-4y8m33ea	174	9	paste	paste	NN
cord-021063-4y8m33ea	174	10	.	.	.
cord-021063-4y8m33ea	175	1	Encapsulation	encapsulation	NN
cord-021063-4y8m33ea	175	2	efficiency	efficiency	NN
cord-021063-4y8m33ea	175	3	is	be	VBZ
cord-021063-4y8m33ea	175	4	high	high	JJ
cord-021063-4y8m33ea	175	5	(	(	-LRB-
cord-021063-4y8m33ea	175	6	up	up	IN
cord-021063-4y8m33ea	175	7	to	to	TO
cord-021063-4y8m33ea	175	8	40	40	CD
cord-021063-4y8m33ea	175	9	or	or	CC
cord-021063-4y8m33ea	175	10	50	50	CD
cord-021063-4y8m33ea	175	11	%	%	NN
cord-021063-4y8m33ea	175	12	)	)	-RRB-
cord-021063-4y8m33ea	175	13	because	because	IN
cord-021063-4y8m33ea	175	14	there	there	EX
cord-021063-4y8m33ea	175	15	is	be	VBZ
cord-021063-4y8m33ea	175	16	only	only	RB
cord-021063-4y8m33ea	175	17	a	a	DT
cord-021063-4y8m33ea	175	18	small	small	JJ
cord-021063-4y8m33ea	175	19	amount	amount	NN
cord-021063-4y8m33ea	175	20	of	of	IN
cord-021063-4y8m33ea	175	21	aqueous	aqueous	JJ
cord-021063-4y8m33ea	175	22	buffer	buffer	NN
cord-021063-4y8m33ea	175	23	present	present	JJ
cord-021063-4y8m33ea	175	24	in	in	IN
cord-021063-4y8m33ea	175	25	relation	relation	NN
cord-021063-4y8m33ea	175	26	to	to	IN
cord-021063-4y8m33ea	175	27	the	the	DT
cord-021063-4y8m33ea	175	28	amount	amount	NN
cord-021063-4y8m33ea	175	29	of	of	IN
cord-021063-4y8m33ea	175	30	lipid	lipid	NN
cord-021063-4y8m33ea	175	31	.	.	.
cord-021063-4y8m33ea	176	1	Presumably	presumably	RB
cord-021063-4y8m33ea	176	2	,	,	,
cord-021063-4y8m33ea	176	3	DNA	dna	NN
cord-021063-4y8m33ea	176	4	molecules	molecule	NNS
cord-021063-4y8m33ea	176	5	present	present	JJ
cord-021063-4y8m33ea	176	6	in	in	IN
cord-021063-4y8m33ea	176	7	the	the	DT
cord-021063-4y8m33ea	176	8	buffer	buffer	NN
cord-021063-4y8m33ea	176	9	are	be	VBP
cord-021063-4y8m33ea	176	10	forced	force	VBN
cord-021063-4y8m33ea	176	11	to	to	TO
cord-021063-4y8m33ea	176	12	curl	curl	VB
cord-021063-4y8m33ea	176	13	up	up	RP
cord-021063-4y8m33ea	176	14	in	in	IN
cord-021063-4y8m33ea	176	15	small	small	JJ
cord-021063-4y8m33ea	176	16	aqueous	aqueous	JJ
cord-021063-4y8m33ea	176	17	droplets	droplet	NNS
cord-021063-4y8m33ea	176	18	,	,	,
cord-021063-4y8m33ea	176	19	a	a	DT
cord-021063-4y8m33ea	176	20	conformation	conformation	NN
cord-021063-4y8m33ea	176	21	that	that	WDT
cord-021063-4y8m33ea	176	22	is	be	VBZ
cord-021063-4y8m33ea	176	23	easily	easily	RB
cord-021063-4y8m33ea	176	24	entrapped	entrap	VBN
cord-021063-4y8m33ea	176	25	(	(	-LRB-
cord-021063-4y8m33ea	176	26	Szelei	Szelei	NNP
cord-021063-4y8m33ea	176	27	and	and	CC
cord-021063-4y8m33ea	176	28	Duda	Duda	NNP
cord-021063-4y8m33ea	176	29	,	,	,
cord-021063-4y8m33ea	176	30	1989	1989	CD
cord-021063-4y8m33ea	176	31	)	)	-RRB-
cord-021063-4y8m33ea	176	32	.	.	.
cord-021063-4y8m33ea	177	1	Because	because	IN
cord-021063-4y8m33ea	177	2	the	the	DT
cord-021063-4y8m33ea	177	3	size	size	NN
cord-021063-4y8m33ea	177	4	distribution	distribution	NN
cord-021063-4y8m33ea	177	5	of	of	IN
cord-021063-4y8m33ea	177	6	the	the	DT
cord-021063-4y8m33ea	177	7	REVs	rev	NNS
cord-021063-4y8m33ea	177	8	is	be	VBZ
cord-021063-4y8m33ea	177	9	heterogeneous	heterogeneous	JJ
cord-021063-4y8m33ea	177	10	and	and	CC
cord-021063-4y8m33ea	177	11	hard	hard	JJ
cord-021063-4y8m33ea	177	12	to	to	TO
cord-021063-4y8m33ea	177	13	control	control	VB
cord-021063-4y8m33ea	177	14	,	,	,
cord-021063-4y8m33ea	177	15	they	-PRON-	PRP
cord-021063-4y8m33ea	177	16	are	be	VBP
cord-021063-4y8m33ea	177	17	often	often	RB
cord-021063-4y8m33ea	177	18	sized	size	VBN
cord-021063-4y8m33ea	177	19	by	by	IN
cord-021063-4y8m33ea	177	20	extrusion	extrusion	NN
cord-021063-4y8m33ea	177	21	through	through	IN
cord-021063-4y8m33ea	177	22	a	a	DT
cord-021063-4y8m33ea	177	23	filter	filter	NN
cord-021063-4y8m33ea	177	24	before	before	IN
cord-021063-4y8m33ea	177	25	use	use	NN
cord-021063-4y8m33ea	177	26	.	.	.
cord-021063-4y8m33ea	178	1	Extrusion	extrusion	NN
cord-021063-4y8m33ea	178	2	.	.	.
cord-021063-4y8m33ea	179	1	Multilamellar	multilamellar	JJ
cord-021063-4y8m33ea	179	2	vesicles	vesicle	NNS
cord-021063-4y8m33ea	179	3	formed	form	VBN
cord-021063-4y8m33ea	179	4	by	by	IN
cord-021063-4y8m33ea	179	5	vortexing	vortexe	VBG
cord-021063-4y8m33ea	179	6	a	a	DT
cord-021063-4y8m33ea	179	7	hydrated	hydrated	JJ
cord-021063-4y8m33ea	179	8	lipid	lipid	NN
cord-021063-4y8m33ea	179	9	film	film	NN
cord-021063-4y8m33ea	179	10	are	be	VBP
cord-021063-4y8m33ea	179	11	repeatedly	repeatedly	RB
cord-021063-4y8m33ea	179	12	passed	pass	VBN
cord-021063-4y8m33ea	179	13	(	(	-LRB-
cord-021063-4y8m33ea	179	14	extruded	extrude	VBN
cord-021063-4y8m33ea	179	15	)	)	-RRB-
cord-021063-4y8m33ea	179	16	through	through	IN
cord-021063-4y8m33ea	179	17	a	a	DT
cord-021063-4y8m33ea	179	18	filter	filter	NN
cord-021063-4y8m33ea	179	19	under	under	IN
cord-021063-4y8m33ea	179	20	pressure	pressure	NN
cord-021063-4y8m33ea	179	21	.	.	.
cord-021063-4y8m33ea	180	1	As	as	IN
cord-021063-4y8m33ea	180	2	the	the	DT
cord-021063-4y8m33ea	180	3	liposomes	liposome	NNS
cord-021063-4y8m33ea	180	4	are	be	VBP
cord-021063-4y8m33ea	180	5	forced	force	VBN
cord-021063-4y8m33ea	180	6	through	through	IN
cord-021063-4y8m33ea	180	7	the	the	DT
cord-021063-4y8m33ea	180	8	pores	pore	NNS
cord-021063-4y8m33ea	180	9	of	of	IN
cord-021063-4y8m33ea	180	10	the	the	DT
cord-021063-4y8m33ea	180	11	filter	filter	NN
cord-021063-4y8m33ea	180	12	,	,	,
cord-021063-4y8m33ea	180	13	they	-PRON-	PRP
cord-021063-4y8m33ea	180	14	are	be	VBP
cord-021063-4y8m33ea	180	15	converted	convert	VBN
cord-021063-4y8m33ea	180	16	from	from	IN
cord-021063-4y8m33ea	180	17	multilamellar	multilamellar	NNP
cord-021063-4y8m33ea	180	18	to	to	IN
cord-021063-4y8m33ea	180	19	unilamellar	unilamellar	NNP
cord-021063-4y8m33ea	180	20	vesicles	vesicle	NNS
cord-021063-4y8m33ea	180	21	,	,	,
cord-021063-4y8m33ea	180	22	and	and	CC
cord-021063-4y8m33ea	180	23	the	the	DT
cord-021063-4y8m33ea	180	24	buffer	buffer	NN
cord-021063-4y8m33ea	180	25	and	and	CC
cord-021063-4y8m33ea	180	26	anything	anything	NN
cord-021063-4y8m33ea	180	27	present	present	JJ
cord-021063-4y8m33ea	180	28	in	in	IN
cord-021063-4y8m33ea	180	29	it	-PRON-	PRP
cord-021063-4y8m33ea	180	30	is	be	VBZ
cord-021063-4y8m33ea	180	31	trapped	trap	VBN
cord-021063-4y8m33ea	180	32	inside	inside	IN
cord-021063-4y8m33ea	180	33	the	the	DT
cord-021063-4y8m33ea	180	34	liposomes	liposome	NNS
cord-021063-4y8m33ea	180	35	(	(	-LRB-
cord-021063-4y8m33ea	180	36	Mayer	Mayer	NNP
cord-021063-4y8m33ea	180	37	et	et	NNP
cord-021063-4y8m33ea	180	38	al	al	NNP
cord-021063-4y8m33ea	180	39	.	.	NNP
cord-021063-4y8m33ea	180	40	,	,	,
cord-021063-4y8m33ea	180	41	1986	1986	CD
cord-021063-4y8m33ea	180	42	)	)	-RRB-
cord-021063-4y8m33ea	180	43	.	.	.
cord-021063-4y8m33ea	181	1	To	to	IN
cord-021063-4y8m33ea	181	2	a	a	DT
cord-021063-4y8m33ea	181	3	certain	certain	JJ
cord-021063-4y8m33ea	181	4	extent	extent	NN
cord-021063-4y8m33ea	181	5	,	,	,
cord-021063-4y8m33ea	181	6	the	the	DT
cord-021063-4y8m33ea	181	7	size	size	NN
cord-021063-4y8m33ea	181	8	of	of	IN
cord-021063-4y8m33ea	181	9	the	the	DT
cord-021063-4y8m33ea	181	10	liposomes	liposome	NNS
cord-021063-4y8m33ea	181	11	corresponds	correspond	VBZ
cord-021063-4y8m33ea	181	12	to	to	IN
cord-021063-4y8m33ea	181	13	the	the	DT
cord-021063-4y8m33ea	181	14	pore	pore	NN
cord-021063-4y8m33ea	181	15	size	size	NN
cord-021063-4y8m33ea	181	16	of	of	IN
cord-021063-4y8m33ea	181	17	the	the	DT
cord-021063-4y8m33ea	181	18	filter	filter	NN
cord-021063-4y8m33ea	181	19	used	use	VBN
cord-021063-4y8m33ea	181	20	.	.	.
cord-021063-4y8m33ea	182	1	Advantages	advantage	NNS
cord-021063-4y8m33ea	182	2	of	of	IN
cord-021063-4y8m33ea	182	3	this	this	DT
cord-021063-4y8m33ea	182	4	technique	technique	NN
cord-021063-4y8m33ea	182	5	are	be	VBP
cord-021063-4y8m33ea	182	6	:	:	:
cord-021063-4y8m33ea	182	7	(	(	-LRB-
cord-021063-4y8m33ea	182	8	i	i	NN
cord-021063-4y8m33ea	182	9	)	)	-RRB-
cord-021063-4y8m33ea	182	10	nearly	nearly	RB
cord-021063-4y8m33ea	182	11	any	any	DT
cord-021063-4y8m33ea	182	12	lipid	lipid	NN
cord-021063-4y8m33ea	182	13	or	or	CC
cord-021063-4y8m33ea	182	14	lipid	lipid	NN
cord-021063-4y8m33ea	182	15	mixture	mixture	NN
cord-021063-4y8m33ea	182	16	can	can	MD
cord-021063-4y8m33ea	182	17	be	be	VB
cord-021063-4y8m33ea	182	18	used	use	VBN
cord-021063-4y8m33ea	182	19	,	,	,
cord-021063-4y8m33ea	182	20	(	(	-LRB-
cord-021063-4y8m33ea	182	21	ii	ii	NN
cord-021063-4y8m33ea	182	22	)	)	-RRB-
cord-021063-4y8m33ea	182	23	liposome	liposome	NN
cord-021063-4y8m33ea	182	24	size	size	NN
cord-021063-4y8m33ea	182	25	from	from	IN
cord-021063-4y8m33ea	182	26	25	25	CD
cord-021063-4y8m33ea	182	27	to	to	TO
cord-021063-4y8m33ea	182	28	100	100	CD
cord-021063-4y8m33ea	182	29	nm	nm	NN
cord-021063-4y8m33ea	182	30	in	in	IN
cord-021063-4y8m33ea	182	31	diameter	diameter	NN
cord-021063-4y8m33ea	182	32	can	can	MD
cord-021063-4y8m33ea	182	33	be	be	VB
cord-021063-4y8m33ea	182	34	controlled	control	VBN
cord-021063-4y8m33ea	182	35	,	,	,
cord-021063-4y8m33ea	182	36	and	and	CC
cord-021063-4y8m33ea	182	37	(	(	-LRB-
cord-021063-4y8m33ea	182	38	iii	iii	NN
cord-021063-4y8m33ea	182	39	)	)	-RRB-
cord-021063-4y8m33ea	183	1	the	the	DT
cord-021063-4y8m33ea	183	2	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	183	3	efficiency	efficiency	NN
cord-021063-4y8m33ea	183	4	is	be	VBZ
cord-021063-4y8m33ea	183	5	excellent	excellent	JJ
cord-021063-4y8m33ea	183	6	(	(	-LRB-
cord-021063-4y8m33ea	183	7	up	up	IN
cord-021063-4y8m33ea	183	8	to	to	TO
cord-021063-4y8m33ea	183	9	30	30	CD
cord-021063-4y8m33ea	183	10	%	%	NN
cord-021063-4y8m33ea	183	11	)	)	-RRB-
cord-021063-4y8m33ea	183	12	.	.	.
cord-021063-4y8m33ea	184	1	The	the	DT
cord-021063-4y8m33ea	184	2	main	main	JJ
cord-021063-4y8m33ea	184	3	disadvantage	disadvantage	NN
cord-021063-4y8m33ea	184	4	is	be	VBZ
cord-021063-4y8m33ea	184	5	that	that	IN
cord-021063-4y8m33ea	184	6	the	the	DT
cord-021063-4y8m33ea	184	7	high	high	JJ
cord-021063-4y8m33ea	184	8	pressures	pressure	NNS
cord-021063-4y8m33ea	184	9	used	use	VBN
cord-021063-4y8m33ea	184	10	in	in	IN
cord-021063-4y8m33ea	184	11	the	the	DT
cord-021063-4y8m33ea	184	12	extrusion	extrusion	NN
cord-021063-4y8m33ea	184	13	process	process	NN
cord-021063-4y8m33ea	184	14	can	can	MD
cord-021063-4y8m33ea	184	15	fragment	fragment	VB
cord-021063-4y8m33ea	184	16	the	the	DT
cord-021063-4y8m33ea	184	17	DNA	dna	NN
cord-021063-4y8m33ea	184	18	,	,	,
cord-021063-4y8m33ea	184	19	and	and	CC
cord-021063-4y8m33ea	184	20	denature	denature	VB
cord-021063-4y8m33ea	184	21	or	or	CC
cord-021063-4y8m33ea	184	22	dissociate	dissociate	VB
cord-021063-4y8m33ea	184	23	DNA	dna	NN
cord-021063-4y8m33ea	184	24	-	-	HYPH
cord-021063-4y8m33ea	184	25	protein	protein	NN
cord-021063-4y8m33ea	184	26	complexes	complex	NNS
cord-021063-4y8m33ea	184	27	.	.	.
cord-021063-4y8m33ea	185	1	A	a	DT
cord-021063-4y8m33ea	185	2	central	central	JJ
cord-021063-4y8m33ea	185	3	difficulty	difficulty	NN
cord-021063-4y8m33ea	185	4	in	in	IN
cord-021063-4y8m33ea	185	5	using	use	VBG
cord-021063-4y8m33ea	185	6	liposomes	liposome	NNS
cord-021063-4y8m33ea	185	7	for	for	IN
cord-021063-4y8m33ea	185	8	gene	gene	NN
cord-021063-4y8m33ea	185	9	therapy	therapy	NN
cord-021063-4y8m33ea	185	10	is	be	VBZ
cord-021063-4y8m33ea	185	11	the	the	DT
cord-021063-4y8m33ea	185	12	entrapment	entrapment	NN
cord-021063-4y8m33ea	185	13	of	of	IN
cord-021063-4y8m33ea	185	14	the	the	DT
cord-021063-4y8m33ea	185	15	DNA	dna	NN
cord-021063-4y8m33ea	185	16	.	.	.
cord-021063-4y8m33ea	186	1	The	the	DT
cord-021063-4y8m33ea	186	2	ideal	ideal	JJ
cord-021063-4y8m33ea	186	3	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	186	4	method	method	NN
cord-021063-4y8m33ea	186	5	would	would	MD
cord-021063-4y8m33ea	186	6	efficiently	efficiently	RB
cord-021063-4y8m33ea	186	7	entrap	entrap	VB
cord-021063-4y8m33ea	186	8	but	but	CC
cord-021063-4y8m33ea	186	9	not	not	RB
cord-021063-4y8m33ea	186	10	degrade	degrade	VB
cord-021063-4y8m33ea	186	11	the	the	DT
cord-021063-4y8m33ea	186	12	DNA	dna	NN
cord-021063-4y8m33ea	186	13	.	.	.
cord-021063-4y8m33ea	187	1	At	at	IN
cord-021063-4y8m33ea	187	2	the	the	DT
cord-021063-4y8m33ea	187	3	same	same	JJ
cord-021063-4y8m33ea	187	4	time	time	NN
cord-021063-4y8m33ea	187	5	,	,	,
cord-021063-4y8m33ea	187	6	the	the	DT
cord-021063-4y8m33ea	187	7	method	method	NN
cord-021063-4y8m33ea	187	8	would	would	MD
cord-021063-4y8m33ea	187	9	be	be	VB
cord-021063-4y8m33ea	187	10	sufficiently	sufficiently	RB
cord-021063-4y8m33ea	187	11	flexible	flexible	JJ
cord-021063-4y8m33ea	187	12	with	with	IN
cord-021063-4y8m33ea	187	13	regard	regard	NN
cord-021063-4y8m33ea	187	14	to	to	IN
cord-021063-4y8m33ea	187	15	size	size	NN
cord-021063-4y8m33ea	187	16	and	and	CC
cord-021063-4y8m33ea	187	17	vesicle	vesicle	NN
cord-021063-4y8m33ea	187	18	composition	composition	NN
cord-021063-4y8m33ea	187	19	to	to	TO
cord-021063-4y8m33ea	187	20	allow	allow	VB
cord-021063-4y8m33ea	187	21	targeting	targeting	NN
cord-021063-4y8m33ea	187	22	of	of	IN
cord-021063-4y8m33ea	187	23	liposomes	liposome	NNS
cord-021063-4y8m33ea	187	24	based	base	VBN
cord-021063-4y8m33ea	187	25	on	on	IN
cord-021063-4y8m33ea	187	26	these	these	DT
cord-021063-4y8m33ea	187	27	properties	property	NNS
cord-021063-4y8m33ea	187	28	.	.	.
cord-021063-4y8m33ea	188	1	DNA	DNA	NNP
cord-021063-4y8m33ea	188	2	in	in	IN
cord-021063-4y8m33ea	188	3	solution	solution	NN
cord-021063-4y8m33ea	188	4	has	have	VBZ
cord-021063-4y8m33ea	188	5	a	a	DT
cord-021063-4y8m33ea	188	6	linear	linear	JJ
cord-021063-4y8m33ea	188	7	conformation	conformation	NN
cord-021063-4y8m33ea	188	8	.	.	.
cord-021063-4y8m33ea	189	1	In	in	IN
cord-021063-4y8m33ea	189	2	general	general	JJ
cord-021063-4y8m33ea	189	3	,	,	,
cord-021063-4y8m33ea	189	4	DNA	dna	NN
cord-021063-4y8m33ea	189	5	restriction	restriction	NN
cord-021063-4y8m33ea	189	6	fragments	fragment	NNS
cord-021063-4y8m33ea	189	7	containing	contain	VBG
cord-021063-4y8m33ea	189	8	an	an	DT
cord-021063-4y8m33ea	189	9	entire	entire	JJ
cord-021063-4y8m33ea	189	10	gene	gene	NN
cord-021063-4y8m33ea	189	11	are	be	VBP
cord-021063-4y8m33ea	189	12	much	much	RB
cord-021063-4y8m33ea	189	13	longer	long	JJR
cord-021063-4y8m33ea	189	14	than	than	IN
cord-021063-4y8m33ea	189	15	the	the	DT
cord-021063-4y8m33ea	189	16	diameter	diameter	NN
cord-021063-4y8m33ea	189	17	of	of	IN
cord-021063-4y8m33ea	189	18	com	com	NN
cord-021063-4y8m33ea	189	19	-	-	HYPH
cord-021063-4y8m33ea	189	20	monly	monly	NN
cord-021063-4y8m33ea	189	21	used	use	VBN
cord-021063-4y8m33ea	189	22	liposomes	liposome	NNS
cord-021063-4y8m33ea	189	23	.	.	.
cord-021063-4y8m33ea	190	1	It	-PRON-	PRP
cord-021063-4y8m33ea	190	2	should	should	MD
cord-021063-4y8m33ea	190	3	not	not	RB
cord-021063-4y8m33ea	190	4	be	be	VB
cord-021063-4y8m33ea	190	5	surprising	surprising	JJ
cord-021063-4y8m33ea	190	6	then	then	RB
cord-021063-4y8m33ea	190	7	that	that	IN
cord-021063-4y8m33ea	190	8	most	most	JJS
cord-021063-4y8m33ea	190	9	methods	method	NNS
cord-021063-4y8m33ea	190	10	of	of	IN
cord-021063-4y8m33ea	190	11	liposome	liposome	NN
cord-021063-4y8m33ea	190	12	formation	formation	NN
cord-021063-4y8m33ea	190	13	entrap	entrap	VBP
cord-021063-4y8m33ea	190	14	DNA	dna	NN
cord-021063-4y8m33ea	190	15	only	only	RB
cord-021063-4y8m33ea	190	16	at	at	IN
cord-021063-4y8m33ea	190	17	low	low	JJ
cord-021063-4y8m33ea	190	18	efficiency	efficiency	NN
cord-021063-4y8m33ea	190	19	,	,	,
cord-021063-4y8m33ea	190	20	and	and	CC
cord-021063-4y8m33ea	190	21	that	that	IN
cord-021063-4y8m33ea	190	22	the	the	DT
cord-021063-4y8m33ea	190	23	efficiency	efficiency	NN
cord-021063-4y8m33ea	190	24	is	be	VBZ
cord-021063-4y8m33ea	190	25	inversely	inversely	RB
cord-021063-4y8m33ea	190	26	related	relate	VBN
cord-021063-4y8m33ea	190	27	to	to	IN
cord-021063-4y8m33ea	190	28	the	the	DT
cord-021063-4y8m33ea	190	29	length	length	NN
cord-021063-4y8m33ea	190	30	of	of	IN
cord-021063-4y8m33ea	190	31	the	the	DT
cord-021063-4y8m33ea	190	32	DNA	dna	NN
cord-021063-4y8m33ea	190	33	(	(	-LRB-
cord-021063-4y8m33ea	190	34	Fraley	Fraley	NNP
cord-021063-4y8m33ea	190	35	et	et	NNP
cord-021063-4y8m33ea	190	36	al	al	NNP
cord-021063-4y8m33ea	190	37	.	.	NNP
cord-021063-4y8m33ea	190	38	,	,	,
cord-021063-4y8m33ea	190	39	1980	1980	CD
cord-021063-4y8m33ea	190	40	)	)	-RRB-
cord-021063-4y8m33ea	190	41	.	.	.
cord-021063-4y8m33ea	191	1	Two	two	CD
cord-021063-4y8m33ea	191	2	methods	method	NNS
cord-021063-4y8m33ea	191	3	of	of	IN
cord-021063-4y8m33ea	191	4	increasing	increase	VBG
cord-021063-4y8m33ea	191	5	the	the	DT
cord-021063-4y8m33ea	191	6	entrapment	entrapment	NN
cord-021063-4y8m33ea	191	7	efficiency	efficiency	NN
cord-021063-4y8m33ea	191	8	of	of	IN
cord-021063-4y8m33ea	191	9	DNA	DNA	NNP
cord-021063-4y8m33ea	191	10	have	have	VBP
cord-021063-4y8m33ea	191	11	been	be	VBN
cord-021063-4y8m33ea	191	12	developed	develop	VBN
cord-021063-4y8m33ea	191	13	.	.	.
cord-021063-4y8m33ea	192	1	In	in	IN
cord-021063-4y8m33ea	192	2	the	the	DT
cord-021063-4y8m33ea	192	3	first	first	JJ
cord-021063-4y8m33ea	192	4	,	,	,
cord-021063-4y8m33ea	192	5	the	the	DT
cord-021063-4y8m33ea	192	6	liposomes	liposome	NNS
cord-021063-4y8m33ea	192	7	are	be	VBP
cord-021063-4y8m33ea	192	8	formed	form	VBN
cord-021063-4y8m33ea	192	9	in	in	IN
cord-021063-4y8m33ea	192	10	such	such	PDT
cord-021063-4y8m33ea	192	11	a	a	DT
cord-021063-4y8m33ea	192	12	way	way	NN
cord-021063-4y8m33ea	192	13	that	that	WDT
cord-021063-4y8m33ea	192	14	the	the	DT
cord-021063-4y8m33ea	192	15	aqueous	aqueous	JJ
cord-021063-4y8m33ea	192	16	phase	phase	NN
cord-021063-4y8m33ea	192	17	,	,	,
cord-021063-4y8m33ea	192	18	containing	contain	VBG
cord-021063-4y8m33ea	192	19	the	the	DT
cord-021063-4y8m33ea	192	20	DNA	dna	NN
cord-021063-4y8m33ea	192	21	,	,	,
cord-021063-4y8m33ea	192	22	is	be	VBZ
cord-021063-4y8m33ea	192	23	broken	break	VBN
cord-021063-4y8m33ea	192	24	into	into	IN
cord-021063-4y8m33ea	192	25	droplets	droplet	NNS
cord-021063-4y8m33ea	192	26	small	small	JJ
cord-021063-4y8m33ea	192	27	enough	enough	RB
cord-021063-4y8m33ea	192	28	such	such	JJ
cord-021063-4y8m33ea	192	29	that	that	IN
cord-021063-4y8m33ea	192	30	the	the	DT
cord-021063-4y8m33ea	192	31	DNA	dna	NN
cord-021063-4y8m33ea	192	32	is	be	VBZ
cord-021063-4y8m33ea	192	33	forced	force	VBN
cord-021063-4y8m33ea	192	34	to	to	TO
cord-021063-4y8m33ea	192	35	curl	curl	VB
cord-021063-4y8m33ea	192	36	in	in	RP
cord-021063-4y8m33ea	192	37	upon	upon	IN
cord-021063-4y8m33ea	192	38	itself	-PRON-	PRP
cord-021063-4y8m33ea	192	39	to	to	TO
cord-021063-4y8m33ea	192	40	remain	remain	VB
cord-021063-4y8m33ea	192	41	within	within	IN
cord-021063-4y8m33ea	192	42	the	the	DT
cord-021063-4y8m33ea	192	43	droplet	droplet	NN
cord-021063-4y8m33ea	192	44	.	.	.
cord-021063-4y8m33ea	193	1	This	this	DT
cord-021063-4y8m33ea	193	2	probably	probably	RB
cord-021063-4y8m33ea	193	3	occurs	occur	VBZ
cord-021063-4y8m33ea	193	4	during	during	IN
cord-021063-4y8m33ea	193	5	REV	REV	NNP
cord-021063-4y8m33ea	193	6	formation	formation	NN
cord-021063-4y8m33ea	193	7	.	.	.
cord-021063-4y8m33ea	194	1	The	the	DT
cord-021063-4y8m33ea	194	2	other	other	JJ
cord-021063-4y8m33ea	194	3	method	method	NN
cord-021063-4y8m33ea	194	4	achieves	achieve	VBZ
cord-021063-4y8m33ea	194	5	the	the	DT
cord-021063-4y8m33ea	194	6	same	same	JJ
cord-021063-4y8m33ea	194	7	result	result	NN
cord-021063-4y8m33ea	194	8	by	by	IN
cord-021063-4y8m33ea	194	9	complexing	complexe	VBG
cord-021063-4y8m33ea	194	10	the	the	DT
cord-021063-4y8m33ea	194	11	DNA	dna	NN
cord-021063-4y8m33ea	194	12	with	with	IN
cord-021063-4y8m33ea	194	13	some	some	DT
cord-021063-4y8m33ea	194	14	substance	substance	NN
cord-021063-4y8m33ea	194	15	in	in	IN
cord-021063-4y8m33ea	194	16	a	a	DT
cord-021063-4y8m33ea	194	17	tight	tight	JJ
cord-021063-4y8m33ea	194	18	and	and	CC
cord-021063-4y8m33ea	194	19	compact	compact	JJ
cord-021063-4y8m33ea	194	20	aggregate	aggregate	NN
cord-021063-4y8m33ea	194	21	.	.	.
cord-021063-4y8m33ea	195	1	To	to	IN
cord-021063-4y8m33ea	195	2	date	date	NN
cord-021063-4y8m33ea	195	3	,	,	,
cord-021063-4y8m33ea	195	4	this	this	DT
cord-021063-4y8m33ea	195	5	has	have	VBZ
cord-021063-4y8m33ea	195	6	been	be	VBN
cord-021063-4y8m33ea	195	7	done	do	VBN
cord-021063-4y8m33ea	195	8	with	with	IN
cord-021063-4y8m33ea	195	9	spermine	spermine	FW
cord-021063-4y8m33ea	195	10	(	(	-LRB-
cord-021063-4y8m33ea	195	11	Tikchonenko	Tikchonenko	NNP
cord-021063-4y8m33ea	195	12	et	et	FW
cord-021063-4y8m33ea	195	13	al	al	NNP
cord-021063-4y8m33ea	195	14	.	.	NNP
cord-021063-4y8m33ea	195	15	,	,	,
cord-021063-4y8m33ea	195	16	1988	1988	CD
cord-021063-4y8m33ea	195	17	)	)	-RRB-
cord-021063-4y8m33ea	195	18	and	and	CC
cord-021063-4y8m33ea	195	19	basic	basic	JJ
cord-021063-4y8m33ea	195	20	proteins	protein	NNS
cord-021063-4y8m33ea	195	21	(	(	-LRB-
cord-021063-4y8m33ea	195	22	Jay	Jay	NNP
cord-021063-4y8m33ea	195	23	and	and	CC
cord-021063-4y8m33ea	195	24	Gilbert	Gilbert	NNP
cord-021063-4y8m33ea	195	25	,	,	,
cord-021063-4y8m33ea	195	26	1987	1987	CD
cord-021063-4y8m33ea	195	27	)	)	-RRB-
cord-021063-4y8m33ea	195	28	.	.	.
cord-021063-4y8m33ea	196	1	The	the	DT
cord-021063-4y8m33ea	196	2	efficiency	efficiency	NN
cord-021063-4y8m33ea	196	3	of	of	IN
cord-021063-4y8m33ea	196	4	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	196	5	can	can	MD
cord-021063-4y8m33ea	196	6	also	also	RB
cord-021063-4y8m33ea	196	7	be	be	VB
cord-021063-4y8m33ea	196	8	increased	increase	VBN
cord-021063-4y8m33ea	196	9	by	by	IN
cord-021063-4y8m33ea	196	10	compacting	compact	VBG
cord-021063-4y8m33ea	196	11	the	the	DT
cord-021063-4y8m33ea	196	12	DNA	dna	NN
cord-021063-4y8m33ea	196	13	in	in	IN
cord-021063-4y8m33ea	196	14	phage	phage	NN
cord-021063-4y8m33ea	196	15	head	head	NN
cord-021063-4y8m33ea	196	16	particles	particle	NNS
cord-021063-4y8m33ea	196	17	(	(	-LRB-
cord-021063-4y8m33ea	196	18	Szelei	Szelei	NNP
cord-021063-4y8m33ea	196	19	and	and	CC
cord-021063-4y8m33ea	196	20	Duda	Duda	NNP
cord-021063-4y8m33ea	196	21	,	,	,
cord-021063-4y8m33ea	196	22	1989	1989	CD
cord-021063-4y8m33ea	196	23	)	)	-RRB-
cord-021063-4y8m33ea	196	24	before	before	IN
cord-021063-4y8m33ea	196	25	liposomal	liposomal	JJ
cord-021063-4y8m33ea	196	26	entrapment	entrapment	NN
cord-021063-4y8m33ea	196	27	.	.	.
cord-021063-4y8m33ea	197	1	The	the	DT
cord-021063-4y8m33ea	197	2	basis	basis	NN
cord-021063-4y8m33ea	197	3	for	for	IN
cord-021063-4y8m33ea	197	4	successful	successful	JJ
cord-021063-4y8m33ea	197	5	transfections	transfection	NNS
cord-021063-4y8m33ea	197	6	by	by	IN
cord-021063-4y8m33ea	197	7	liposomal	liposomal	NN
cord-021063-4y8m33ea	197	8	vectors	vector	NNS
cord-021063-4y8m33ea	197	9	delivered	deliver	VBD
cord-021063-4y8m33ea	197	10	intravenously	intravenously	RB
cord-021063-4y8m33ea	197	11	lies	lie	VBZ
cord-021063-4y8m33ea	197	12	in	in	IN
cord-021063-4y8m33ea	197	13	the	the	DT
cord-021063-4y8m33ea	197	14	maximization	maximization	NN
cord-021063-4y8m33ea	197	15	of	of	IN
cord-021063-4y8m33ea	197	16	the	the	DT
cord-021063-4y8m33ea	197	17	in	in	IN
cord-021063-4y8m33ea	197	18	vivo	vivo	NNP
cord-021063-4y8m33ea	197	19	circulation	circulation	NN
cord-021063-4y8m33ea	197	20	time	time	NN
cord-021063-4y8m33ea	197	21	of	of	IN
cord-021063-4y8m33ea	197	22	the	the	DT
cord-021063-4y8m33ea	197	23	liposomes	liposome	NNS
cord-021063-4y8m33ea	197	24	.	.	.
cord-021063-4y8m33ea	198	1	Most	Most	JJS
cord-021063-4y8m33ea	198	2	liposome	liposome	NN
cord-021063-4y8m33ea	198	3	formulations	formulation	NNS
cord-021063-4y8m33ea	198	4	are	be	VBP
cord-021063-4y8m33ea	198	5	cleared	clear	VBN
cord-021063-4y8m33ea	198	6	rapidly	rapidly	RB
cord-021063-4y8m33ea	198	7	from	from	IN
cord-021063-4y8m33ea	198	8	the	the	DT
cord-021063-4y8m33ea	198	9	circulation	circulation	NN
cord-021063-4y8m33ea	198	10	by	by	IN
cord-021063-4y8m33ea	198	11	the	the	DT
cord-021063-4y8m33ea	198	12	reticuloendothelial	reticuloendothelial	JJ
cord-021063-4y8m33ea	198	13	system	system	NN
cord-021063-4y8m33ea	198	14	(	(	-LRB-
cord-021063-4y8m33ea	198	15	RES	RES	NNP
cord-021063-4y8m33ea	198	16	)	)	-RRB-
cord-021063-4y8m33ea	198	17	,	,	,
cord-021063-4y8m33ea	198	18	with	with	IN
cord-021063-4y8m33ea	198	19	large	large	JJ
cord-021063-4y8m33ea	198	20	amounts	amount	NNS
cord-021063-4y8m33ea	198	21	accumulating	accumulate	VBG
cord-021063-4y8m33ea	198	22	in	in	IN
cord-021063-4y8m33ea	198	23	macrophages	macrophage	NNS
cord-021063-4y8m33ea	198	24	(	(	-LRB-
cord-021063-4y8m33ea	198	25	Allen	Allen	NNP
cord-021063-4y8m33ea	198	26	et	et	NNP
cord-021063-4y8m33ea	198	27	al	al	NNP
cord-021063-4y8m33ea	198	28	.	.	NNP
cord-021063-4y8m33ea	198	29	,	,	,
cord-021063-4y8m33ea	198	30	1989	1989	CD
cord-021063-4y8m33ea	198	31	)	)	-RRB-
cord-021063-4y8m33ea	198	32	.	.	.
cord-021063-4y8m33ea	199	1	If	if	IN
cord-021063-4y8m33ea	199	2	the	the	DT
cord-021063-4y8m33ea	199	3	intended	intend	VBN
cord-021063-4y8m33ea	199	4	target	target	NN
cord-021063-4y8m33ea	199	5	of	of	IN
cord-021063-4y8m33ea	199	6	the	the	DT
cord-021063-4y8m33ea	199	7	gene	gene	NN
cord-021063-4y8m33ea	199	8	therapy	therapy	NN
cord-021063-4y8m33ea	199	9	is	be	VBZ
cord-021063-4y8m33ea	199	10	the	the	DT
cord-021063-4y8m33ea	199	11	RES	RES	NNP
cord-021063-4y8m33ea	199	12	,	,	,
cord-021063-4y8m33ea	199	13	as	as	IN
cord-021063-4y8m33ea	199	14	in	in	IN
cord-021063-4y8m33ea	199	15	Gaucher	Gaucher	NNP
cord-021063-4y8m33ea	199	16	's	's	POS
cord-021063-4y8m33ea	199	17	disease	disease	NN
cord-021063-4y8m33ea	199	18	,	,	,
cord-021063-4y8m33ea	199	19	this	this	DT
cord-021063-4y8m33ea	199	20	is	be	VBZ
cord-021063-4y8m33ea	199	21	a	a	DT
cord-021063-4y8m33ea	199	22	benefit	benefit	NN
cord-021063-4y8m33ea	199	23	.	.	.
cord-021063-4y8m33ea	200	1	In	in	IN
cord-021063-4y8m33ea	200	2	most	most	JJS
cord-021063-4y8m33ea	200	3	cases	case	NNS
cord-021063-4y8m33ea	200	4	,	,	,
cord-021063-4y8m33ea	200	5	however	however	RB
cord-021063-4y8m33ea	200	6	,	,	,
cord-021063-4y8m33ea	200	7	this	this	DT
cord-021063-4y8m33ea	200	8	fate	fate	NN
cord-021063-4y8m33ea	200	9	will	will	MD
cord-021063-4y8m33ea	200	10	only	only	RB
cord-021063-4y8m33ea	200	11	serve	serve	VB
cord-021063-4y8m33ea	200	12	to	to	TO
cord-021063-4y8m33ea	200	13	reduce	reduce	VB
cord-021063-4y8m33ea	200	14	the	the	DT
cord-021063-4y8m33ea	200	15	number	number	NN
cord-021063-4y8m33ea	200	16	of	of	IN
cord-021063-4y8m33ea	200	17	liposomes	liposome	NNS
cord-021063-4y8m33ea	200	18	being	be	VBG
cord-021063-4y8m33ea	200	19	exposed	expose	VBN
cord-021063-4y8m33ea	200	20	to	to	IN
cord-021063-4y8m33ea	200	21	their	-PRON-	PRP$
cord-021063-4y8m33ea	200	22	intended	intend	VBN
cord-021063-4y8m33ea	200	23	target	target	NN
cord-021063-4y8m33ea	200	24	cell	cell	NN
cord-021063-4y8m33ea	200	25	population	population	NN
cord-021063-4y8m33ea	200	26	.	.	.
cord-021063-4y8m33ea	201	1	While	while	IN
cord-021063-4y8m33ea	201	2	several	several	JJ
cord-021063-4y8m33ea	201	3	different	different	JJ
cord-021063-4y8m33ea	201	4	strategies	strategy	NNS
cord-021063-4y8m33ea	201	5	have	have	VBP
cord-021063-4y8m33ea	201	6	been	be	VBN
cord-021063-4y8m33ea	201	7	explored	explore	VBN
cord-021063-4y8m33ea	201	8	to	to	TO
cord-021063-4y8m33ea	201	9	lengthen	lengthen	VB
cord-021063-4y8m33ea	201	10	the	the	DT
cord-021063-4y8m33ea	201	11	circulation	circulation	NN
cord-021063-4y8m33ea	201	12	half	half	JJ
cord-021063-4y8m33ea	201	13	-	-	HYPH
cord-021063-4y8m33ea	201	14	life	life	NN
cord-021063-4y8m33ea	201	15	of	of	IN
cord-021063-4y8m33ea	201	16	liposomes	liposome	NNS
cord-021063-4y8m33ea	201	17	,	,	,
cord-021063-4y8m33ea	201	18	they	-PRON-	PRP
cord-021063-4y8m33ea	201	19	all	all	DT
cord-021063-4y8m33ea	201	20	have	have	VBP
cord-021063-4y8m33ea	201	21	certain	certain	JJ
cord-021063-4y8m33ea	201	22	aspects	aspect	NNS
cord-021063-4y8m33ea	201	23	in	in	IN
cord-021063-4y8m33ea	201	24	common	common	JJ
cord-021063-4y8m33ea	202	1	(	(	-LRB-
cord-021063-4y8m33ea	202	2	Allen	Allen	NNP
cord-021063-4y8m33ea	202	3	et	et	NNP
cord-021063-4y8m33ea	202	4	al	al	NNP
cord-021063-4y8m33ea	202	5	.	.	.
cord-021063-4y8m33ea	203	1	,	,	,
cord-021063-4y8m33ea	203	2	1989	1989	CD
cord-021063-4y8m33ea	203	3	;	;	:
cord-021063-4y8m33ea	203	4	Gabizon	Gabizon	NNP
cord-021063-4y8m33ea	203	5	and	and	CC
cord-021063-4y8m33ea	203	6	Papahadjopoulos	Papahadjopoulos	NNP
cord-021063-4y8m33ea	203	7	,	,	,
cord-021063-4y8m33ea	203	8	1992	1992	CD
cord-021063-4y8m33ea	203	9	)	)	-RRB-
cord-021063-4y8m33ea	203	10	.	.	.
cord-021063-4y8m33ea	204	1	First	first	RB
cord-021063-4y8m33ea	204	2	,	,	,
cord-021063-4y8m33ea	204	3	they	-PRON-	PRP
cord-021063-4y8m33ea	204	4	seek	seek	VBP
cord-021063-4y8m33ea	204	5	to	to	TO
cord-021063-4y8m33ea	204	6	make	make	VB
cord-021063-4y8m33ea	204	7	the	the	DT
cord-021063-4y8m33ea	204	8	liposomes	liposome	NNS
cord-021063-4y8m33ea	204	9	as	as	RB
cord-021063-4y8m33ea	204	10	small	small	JJ
cord-021063-4y8m33ea	204	11	as	as	IN
cord-021063-4y8m33ea	204	12	possible	possible	JJ
cord-021063-4y8m33ea	204	13	.	.	.
cord-021063-4y8m33ea	205	1	This	this	DT
cord-021063-4y8m33ea	205	2	seems	seem	VBZ
cord-021063-4y8m33ea	205	3	to	to	TO
cord-021063-4y8m33ea	205	4	reduce	reduce	VB
cord-021063-4y8m33ea	205	5	their	-PRON-	PRP$
cord-021063-4y8m33ea	205	6	interaction	interaction	NN
cord-021063-4y8m33ea	205	7	with	with	IN
cord-021063-4y8m33ea	205	8	the	the	DT
cord-021063-4y8m33ea	205	9	RES	res	NN
cord-021063-4y8m33ea	205	10	.	.	.
cord-021063-4y8m33ea	206	1	Second	second	RB
cord-021063-4y8m33ea	206	2	,	,	,
cord-021063-4y8m33ea	206	3	they	-PRON-	PRP
cord-021063-4y8m33ea	206	4	are	be	VBP
cord-021063-4y8m33ea	206	5	designed	design	VBN
cord-021063-4y8m33ea	206	6	to	to	TO
cord-021063-4y8m33ea	206	7	make	make	VB
cord-021063-4y8m33ea	206	8	the	the	DT
cord-021063-4y8m33ea	206	9	liposomal	liposomal	JJ
cord-021063-4y8m33ea	206	10	surface	surface	NN
cord-021063-4y8m33ea	206	11	as	as	RB
cord-021063-4y8m33ea	206	12	much	much	RB
cord-021063-4y8m33ea	206	13	like	like	IN
cord-021063-4y8m33ea	206	14	the	the	DT
cord-021063-4y8m33ea	206	15	surface	surface	NN
cord-021063-4y8m33ea	206	16	of	of	IN
cord-021063-4y8m33ea	206	17	a	a	DT
cord-021063-4y8m33ea	206	18	normal	normal	JJ
cord-021063-4y8m33ea	206	19	cell	cell	NN
cord-021063-4y8m33ea	206	20	as	as	IN
cord-021063-4y8m33ea	206	21	possible	possible	JJ
cord-021063-4y8m33ea	206	22	.	.	.
cord-021063-4y8m33ea	207	1	This	this	DT
cord-021063-4y8m33ea	207	2	can	can	MD
cord-021063-4y8m33ea	207	3	be	be	VB
cord-021063-4y8m33ea	207	4	accomplished	accomplish	VBN
cord-021063-4y8m33ea	207	5	by	by	IN
cord-021063-4y8m33ea	207	6	using	use	VBG
cord-021063-4y8m33ea	207	7	lipid	lipid	NN
cord-021063-4y8m33ea	207	8	formulations	formulation	NNS
cord-021063-4y8m33ea	207	9	containing	contain	VBG
cord-021063-4y8m33ea	207	10	a	a	DT
cord-021063-4y8m33ea	207	11	high	high	JJ
cord-021063-4y8m33ea	207	12	fraction	fraction	NN
cord-021063-4y8m33ea	207	13	of	of	IN
cord-021063-4y8m33ea	207	14	cholesterol	cholesterol	NN
cord-021063-4y8m33ea	207	15	,	,	,
cord-021063-4y8m33ea	207	16	high	high	JJ
cord-021063-4y8m33ea	207	17	transition	transition	NN
cord-021063-4y8m33ea	207	18	temperature	temperature	NN
cord-021063-4y8m33ea	207	19	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	207	20	,	,	,
cord-021063-4y8m33ea	207	21	and	and	CC
cord-021063-4y8m33ea	207	22	a	a	DT
cord-021063-4y8m33ea	207	23	substantial	substantial	JJ
cord-021063-4y8m33ea	207	24	fraction	fraction	NN
cord-021063-4y8m33ea	207	25	of	of	IN
cord-021063-4y8m33ea	207	26	sphingomyelin	sphingomyelin	NN
cord-021063-4y8m33ea	207	27	or	or	CC
cord-021063-4y8m33ea	207	28	the	the	DT
cord-021063-4y8m33ea	207	29	ganglioside	ganglioside	NN
cord-021063-4y8m33ea	207	30	GM	GM	NNP
cord-021063-4y8m33ea	207	31	1	1	CD
cord-021063-4y8m33ea	207	32	.	.	.
cord-021063-4y8m33ea	208	1	A	a	DT
cord-021063-4y8m33ea	208	2	different	different	JJ
cord-021063-4y8m33ea	208	3	approach	approach	NN
cord-021063-4y8m33ea	208	4	has	have	VBZ
cord-021063-4y8m33ea	208	5	been	be	VBN
cord-021063-4y8m33ea	208	6	to	to	TO
cord-021063-4y8m33ea	208	7	modify	modify	VB
cord-021063-4y8m33ea	208	8	the	the	DT
cord-021063-4y8m33ea	208	9	liposomal	liposomal	JJ
cord-021063-4y8m33ea	208	10	phospholipids	phospholipid	NNS
cord-021063-4y8m33ea	208	11	by	by	IN
cord-021063-4y8m33ea	208	12	addition	addition	NN
cord-021063-4y8m33ea	208	13	of	of	IN
cord-021063-4y8m33ea	208	14	polyethylene	polyethylene	NN
cord-021063-4y8m33ea	208	15	glycol	glycol	NN
cord-021063-4y8m33ea	208	16	(	(	-LRB-
cord-021063-4y8m33ea	208	17	PEG	PEG	NNP
cord-021063-4y8m33ea	208	18	)	)	-RRB-
cord-021063-4y8m33ea	208	19	to	to	IN
cord-021063-4y8m33ea	208	20	the	the	DT
cord-021063-4y8m33ea	208	21	polar	polar	JJ
cord-021063-4y8m33ea	208	22	headgroup	headgroup	NN
cord-021063-4y8m33ea	208	23	(	(	-LRB-
cord-021063-4y8m33ea	208	24	Lasic	Lasic	NNP
cord-021063-4y8m33ea	208	25	et	et	FW
cord-021063-4y8m33ea	208	26	al	al	NNP
cord-021063-4y8m33ea	208	27	.	.	NNP
cord-021063-4y8m33ea	208	28	,	,	,
cord-021063-4y8m33ea	208	29	1991	1991	CD
cord-021063-4y8m33ea	208	30	;	;	:
cord-021063-4y8m33ea	208	31	Blume	Blume	NNP
cord-021063-4y8m33ea	208	32	and	and	CC
cord-021063-4y8m33ea	208	33	Cevc	Cevc	NNP
cord-021063-4y8m33ea	208	34	,	,	,
cord-021063-4y8m33ea	208	35	1993	1993	CD
cord-021063-4y8m33ea	208	36	)	)	-RRB-
cord-021063-4y8m33ea	208	37	.	.	.
cord-021063-4y8m33ea	209	1	PEG	peg	NN
cord-021063-4y8m33ea	209	2	-	-	HYPH
cord-021063-4y8m33ea	209	3	modified	modify	VBN
cord-021063-4y8m33ea	209	4	lipids	lipid	NNS
cord-021063-4y8m33ea	209	5	have	have	VBP
cord-021063-4y8m33ea	209	6	a	a	DT
cord-021063-4y8m33ea	209	7	higher	high	JJR
cord-021063-4y8m33ea	209	8	hydrophilicity	hydrophilicity	NN
cord-021063-4y8m33ea	209	9	than	than	IN
cord-021063-4y8m33ea	209	10	native	native	JJ
cord-021063-4y8m33ea	209	11	lipids	lipid	NNS
cord-021063-4y8m33ea	209	12	,	,	,
cord-021063-4y8m33ea	209	13	and	and	CC
cord-021063-4y8m33ea	209	14	appear	appear	VBP
cord-021063-4y8m33ea	209	15	to	to	TO
cord-021063-4y8m33ea	209	16	escape	escape	VB
cord-021063-4y8m33ea	209	17	recognition	recognition	NN
cord-021063-4y8m33ea	209	18	by	by	IN
cord-021063-4y8m33ea	209	19	macrophages	macrophage	NNS
cord-021063-4y8m33ea	209	20	.	.	.
cord-021063-4y8m33ea	210	1	A	a	DT
cord-021063-4y8m33ea	210	2	potential	potential	JJ
cord-021063-4y8m33ea	210	3	disadvantage	disadvantage	NN
cord-021063-4y8m33ea	210	4	of	of	IN
cord-021063-4y8m33ea	210	5	this	this	DT
cord-021063-4y8m33ea	210	6	approach	approach	NN
cord-021063-4y8m33ea	210	7	is	be	VBZ
cord-021063-4y8m33ea	210	8	that	that	IN
cord-021063-4y8m33ea	210	9	targeting	targeting	NN
cord-021063-4y8m33ea	210	10	of	of	IN
cord-021063-4y8m33ea	210	11	liposomes	liposome	NNS
cord-021063-4y8m33ea	210	12	with	with	IN
cord-021063-4y8m33ea	210	13	covalently	covalently	RB
cord-021063-4y8m33ea	210	14	attached	attach	VBN
cord-021063-4y8m33ea	210	15	antibodies	antibody	NNS
cord-021063-4y8m33ea	210	16	(	(	-LRB-
cord-021063-4y8m33ea	210	17	see	see	VB
cord-021063-4y8m33ea	210	18	below	below	RB
cord-021063-4y8m33ea	210	19	)	)	-RRB-
cord-021063-4y8m33ea	210	20	is	be	VBZ
cord-021063-4y8m33ea	210	21	inhibited	inhibit	VBN
cord-021063-4y8m33ea	210	22	by	by	IN
cord-021063-4y8m33ea	210	23	the	the	DT
cord-021063-4y8m33ea	210	24	presence	presence	NN
cord-021063-4y8m33ea	210	25	of	of	IN
cord-021063-4y8m33ea	210	26	PEG	peg	NN
cord-021063-4y8m33ea	210	27	-	-	HYPH
cord-021063-4y8m33ea	210	28	modified	modify	VBN
cord-021063-4y8m33ea	210	29	lipids	lipid	NNS
cord-021063-4y8m33ea	211	1	(	(	-LRB-
cord-021063-4y8m33ea	211	2	Klibanov	Klibanov	NNP
cord-021063-4y8m33ea	211	3	et	et	NNP
cord-021063-4y8m33ea	211	4	al	al	NNP
cord-021063-4y8m33ea	211	5	.	.	NNP
cord-021063-4y8m33ea	211	6	,	,	,
cord-021063-4y8m33ea	211	7	1991	1991	CD
cord-021063-4y8m33ea	211	8	)	)	-RRB-
cord-021063-4y8m33ea	211	9	.	.	.
cord-021063-4y8m33ea	212	1	Once	once	RB
cord-021063-4y8m33ea	212	2	a	a	DT
cord-021063-4y8m33ea	212	3	liposome	liposome	NN
cord-021063-4y8m33ea	212	4	formulation	formulation	NN
cord-021063-4y8m33ea	212	5	has	have	VBZ
cord-021063-4y8m33ea	212	6	been	be	VBN
cord-021063-4y8m33ea	212	7	settled	settle	VBN
cord-021063-4y8m33ea	212	8	upon	upon	IN
cord-021063-4y8m33ea	212	9	,	,	,
cord-021063-4y8m33ea	212	10	attention	attention	NN
cord-021063-4y8m33ea	212	11	must	must	MD
cord-021063-4y8m33ea	212	12	be	be	VB
cord-021063-4y8m33ea	212	13	paid	pay	VBN
cord-021063-4y8m33ea	212	14	to	to	IN
cord-021063-4y8m33ea	212	15	limiting	limit	VBG
cord-021063-4y8m33ea	212	16	the	the	DT
cord-021063-4y8m33ea	212	17	number	number	NN
cord-021063-4y8m33ea	212	18	of	of	IN
cord-021063-4y8m33ea	212	19	cells	cell	NNS
cord-021063-4y8m33ea	212	20	exposed	expose	VBN
cord-021063-4y8m33ea	212	21	to	to	IN
cord-021063-4y8m33ea	212	22	the	the	DT
cord-021063-4y8m33ea	212	23	DNA	dna	NN
cord-021063-4y8m33ea	212	24	delivered	deliver	VBN
cord-021063-4y8m33ea	212	25	by	by	IN
cord-021063-4y8m33ea	212	26	the	the	DT
cord-021063-4y8m33ea	212	27	liposomes	liposome	NNS
cord-021063-4y8m33ea	212	28	.	.	.
cord-021063-4y8m33ea	213	1	This	this	DT
cord-021063-4y8m33ea	213	2	is	be	VBZ
cord-021063-4y8m33ea	213	3	necessary	necessary	JJ
cord-021063-4y8m33ea	213	4	for	for	IN
cord-021063-4y8m33ea	213	5	several	several	JJ
cord-021063-4y8m33ea	213	6	reasons	reason	NNS
cord-021063-4y8m33ea	213	7	.	.	.
cord-021063-4y8m33ea	214	1	First	first	RB
cord-021063-4y8m33ea	214	2	,	,	,
cord-021063-4y8m33ea	214	3	if	if	IN
cord-021063-4y8m33ea	214	4	the	the	DT
cord-021063-4y8m33ea	214	5	DNA	dna	NN
cord-021063-4y8m33ea	214	6	will	will	MD
cord-021063-4y8m33ea	214	7	be	be	VB
cord-021063-4y8m33ea	214	8	integrated	integrate	VBN
cord-021063-4y8m33ea	214	9	into	into	IN
cord-021063-4y8m33ea	214	10	the	the	DT
cord-021063-4y8m33ea	214	11	host	host	NN
cord-021063-4y8m33ea	214	12	cell	cell	NN
cord-021063-4y8m33ea	214	13	's	's	POS
cord-021063-4y8m33ea	214	14	genome	genome	NN
cord-021063-4y8m33ea	214	15	,	,	,
cord-021063-4y8m33ea	214	16	there	there	EX
cord-021063-4y8m33ea	214	17	is	be	VBZ
cord-021063-4y8m33ea	214	18	a	a	DT
cord-021063-4y8m33ea	214	19	chance	chance	NN
cord-021063-4y8m33ea	214	20	that	that	IN
cord-021063-4y8m33ea	214	21	insertional	insertional	JJ
cord-021063-4y8m33ea	214	22	mutagenesis	mutagenesis	NN
cord-021063-4y8m33ea	214	23	will	will	MD
cord-021063-4y8m33ea	214	24	lead	lead	VB
cord-021063-4y8m33ea	214	25	to	to	IN
cord-021063-4y8m33ea	214	26	activation	activation	NN
cord-021063-4y8m33ea	214	27	of	of	IN
cord-021063-4y8m33ea	214	28	a	a	DT
cord-021063-4y8m33ea	214	29	proto	proto	JJ
cord-021063-4y8m33ea	214	30	-	-	HYPH
cord-021063-4y8m33ea	214	31	oncogene	oncogene	NN
cord-021063-4y8m33ea	214	32	and	and	CC
cord-021063-4y8m33ea	214	33	the	the	DT
cord-021063-4y8m33ea	214	34	development	development	NN
cord-021063-4y8m33ea	214	35	of	of	IN
cord-021063-4y8m33ea	214	36	cancer	cancer	NN
cord-021063-4y8m33ea	214	37	by	by	IN
cord-021063-4y8m33ea	214	38	the	the	DT
cord-021063-4y8m33ea	214	39	host	host	NN
cord-021063-4y8m33ea	214	40	.	.	.
cord-021063-4y8m33ea	215	1	By	by	IN
cord-021063-4y8m33ea	215	2	limiting	limit	VBG
cord-021063-4y8m33ea	215	3	the	the	DT
cord-021063-4y8m33ea	215	4	sites	site	NNS
cord-021063-4y8m33ea	215	5	of	of	IN
cord-021063-4y8m33ea	215	6	DNA	dna	NN
cord-021063-4y8m33ea	215	7	delivery	delivery	NN
cord-021063-4y8m33ea	215	8	,	,	,
cord-021063-4y8m33ea	215	9	the	the	DT
cord-021063-4y8m33ea	215	10	chance	chance	NN
cord-021063-4y8m33ea	215	11	of	of	IN
cord-021063-4y8m33ea	215	12	insertional	insertional	JJ
cord-021063-4y8m33ea	215	13	mutagenesis	mutagenesis	NN
cord-021063-4y8m33ea	215	14	is	be	VBZ
cord-021063-4y8m33ea	215	15	lowered	lower	VBN
cord-021063-4y8m33ea	215	16	.	.	.
cord-021063-4y8m33ea	216	1	Second	second	RB
cord-021063-4y8m33ea	216	2	,	,	,
cord-021063-4y8m33ea	216	3	limiting	limit	VBG
cord-021063-4y8m33ea	216	4	the	the	DT
cord-021063-4y8m33ea	216	5	number	number	NN
cord-021063-4y8m33ea	216	6	of	of	IN
cord-021063-4y8m33ea	216	7	cells	cell	NNS
cord-021063-4y8m33ea	216	8	that	that	WDT
cord-021063-4y8m33ea	216	9	are	be	VBP
cord-021063-4y8m33ea	216	10	transfected	transfecte	VBN
cord-021063-4y8m33ea	216	11	reduces	reduce	VBZ
cord-021063-4y8m33ea	216	12	the	the	DT
cord-021063-4y8m33ea	216	13	amount	amount	NN
cord-021063-4y8m33ea	216	14	of	of	IN
cord-021063-4y8m33ea	216	15	DNA	dna	NN
cord-021063-4y8m33ea	216	16	,	,	,
cord-021063-4y8m33ea	216	17	lipid	lipid	NN
cord-021063-4y8m33ea	216	18	,	,	,
cord-021063-4y8m33ea	216	19	and	and	CC
cord-021063-4y8m33ea	216	20	other	other	JJ
cord-021063-4y8m33ea	216	21	proteins	protein	NNS
cord-021063-4y8m33ea	216	22	needed	need	VBN
cord-021063-4y8m33ea	216	23	to	to	TO
cord-021063-4y8m33ea	216	24	perform	perform	VB
cord-021063-4y8m33ea	216	25	the	the	DT
cord-021063-4y8m33ea	216	26	gene	gene	NN
cord-021063-4y8m33ea	216	27	therapy	therapy	NN
cord-021063-4y8m33ea	216	28	.	.	.
cord-021063-4y8m33ea	217	1	This	this	DT
cord-021063-4y8m33ea	217	2	reduction	reduction	NN
cord-021063-4y8m33ea	217	3	can	can	MD
cord-021063-4y8m33ea	217	4	be	be	VB
cord-021063-4y8m33ea	217	5	substantial	substantial	JJ
cord-021063-4y8m33ea	217	6	,	,	,
cord-021063-4y8m33ea	217	7	and	and	CC
cord-021063-4y8m33ea	217	8	can	can	MD
cord-021063-4y8m33ea	217	9	reduce	reduce	VB
cord-021063-4y8m33ea	217	10	potential	potential	JJ
cord-021063-4y8m33ea	217	11	systemic	systemic	JJ
cord-021063-4y8m33ea	217	12	toxicities	toxicity	NNS
cord-021063-4y8m33ea	217	13	,	,	,
cord-021063-4y8m33ea	217	14	as	as	RB
cord-021063-4y8m33ea	217	15	well	well	RB
cord-021063-4y8m33ea	217	16	as	as	IN
cord-021063-4y8m33ea	217	17	cost	cost	NN
cord-021063-4y8m33ea	217	18	.	.	.
cord-021063-4y8m33ea	218	1	Table	table	NN
cord-021063-4y8m33ea	218	2	4	4	CD
cord-021063-4y8m33ea	218	3	compares	compare	VBZ
cord-021063-4y8m33ea	218	4	some	some	DT
cord-021063-4y8m33ea	218	5	different	different	JJ
cord-021063-4y8m33ea	218	6	methods	method	NNS
cord-021063-4y8m33ea	218	7	of	of	IN
cord-021063-4y8m33ea	218	8	targeting	target	VBG
cord-021063-4y8m33ea	218	9	liposomes	liposome	NNS
cord-021063-4y8m33ea	218	10	to	to	IN
cord-021063-4y8m33ea	218	11	specific	specific	JJ
cord-021063-4y8m33ea	218	12	cell	cell	NN
cord-021063-4y8m33ea	218	13	types	type	NNS
cord-021063-4y8m33ea	218	14	.	.	.
cord-021063-4y8m33ea	219	1	There	there	EX
cord-021063-4y8m33ea	219	2	are	be	VBP
cord-021063-4y8m33ea	219	3	three	three	CD
cord-021063-4y8m33ea	219	4	general	general	JJ
cord-021063-4y8m33ea	219	5	methods	method	NNS
cord-021063-4y8m33ea	219	6	by	by	IN
cord-021063-4y8m33ea	219	7	which	which	WDT
cord-021063-4y8m33ea	219	8	liposomes	liposome	NNS
cord-021063-4y8m33ea	219	9	may	may	MD
cord-021063-4y8m33ea	219	10	be	be	VB
cord-021063-4y8m33ea	219	11	directed	direct	VBN
cord-021063-4y8m33ea	219	12	to	to	IN
cord-021063-4y8m33ea	219	13	a	a	DT
cord-021063-4y8m33ea	219	14	subset	subset	NN
cord-021063-4y8m33ea	219	15	of	of	IN
cord-021063-4y8m33ea	219	16	cells	cell	NNS
cord-021063-4y8m33ea	219	17	within	within	IN
cord-021063-4y8m33ea	219	18	an	an	DT
cord-021063-4y8m33ea	219	19	organism	organism	NN
cord-021063-4y8m33ea	219	20	.	.	.
cord-021063-4y8m33ea	220	1	First	first	RB
cord-021063-4y8m33ea	220	2	,	,	,
cord-021063-4y8m33ea	220	3	the	the	DT
cord-021063-4y8m33ea	220	4	liposomes	liposome	NNS
cord-021063-4y8m33ea	220	5	may	may	MD
cord-021063-4y8m33ea	220	6	be	be	VB
cord-021063-4y8m33ea	220	7	introduced	introduce	VBN
cord-021063-4y8m33ea	220	8	into	into	IN
cord-021063-4y8m33ea	220	9	a	a	DT
cord-021063-4y8m33ea	220	10	compartment	compartment	NN
cord-021063-4y8m33ea	220	11	of	of	IN
cord-021063-4y8m33ea	220	12	the	the	DT
cord-021063-4y8m33ea	220	13	body	body	NN
cord-021063-4y8m33ea	220	14	that	that	WDT
cord-021063-4y8m33ea	220	15	is	be	VBZ
cord-021063-4y8m33ea	220	16	sequestered	sequester	VBN
cord-021063-4y8m33ea	220	17	from	from	IN
cord-021063-4y8m33ea	220	18	the	the	DT
cord-021063-4y8m33ea	220	19	general	general	JJ
cord-021063-4y8m33ea	220	20	circulation	circulation	NN
cord-021063-4y8m33ea	220	21	.	.	.
cord-021063-4y8m33ea	221	1	One	one	CD
cord-021063-4y8m33ea	221	2	example	example	NN
cord-021063-4y8m33ea	221	3	of	of	IN
cord-021063-4y8m33ea	221	4	this	this	DT
cord-021063-4y8m33ea	221	5	is	be	VBZ
cord-021063-4y8m33ea	221	6	the	the	DT
cord-021063-4y8m33ea	221	7	addition	addition	NN
cord-021063-4y8m33ea	221	8	of	of	IN
cord-021063-4y8m33ea	221	9	liposomes	liposome	NNS
cord-021063-4y8m33ea	221	10	to	to	IN
cord-021063-4y8m33ea	221	11	the	the	DT
cord-021063-4y8m33ea	221	12	airway	airway	NN
cord-021063-4y8m33ea	221	13	in	in	IN
cord-021063-4y8m33ea	221	14	a	a	DT
cord-021063-4y8m33ea	221	15	fluid	fluid	NN
cord-021063-4y8m33ea	221	16	or	or	CC
cord-021063-4y8m33ea	221	17	an	an	DT
cord-021063-4y8m33ea	221	18	aerosol	aerosol	NN
cord-021063-4y8m33ea	222	1	(	(	-LRB-
cord-021063-4y8m33ea	222	2	Hazinski	Hazinski	NNP
cord-021063-4y8m33ea	222	3	et	et	NNP
cord-021063-4y8m33ea	222	4	al	al	NNP
cord-021063-4y8m33ea	222	5	.	.	NNP
cord-021063-4y8m33ea	222	6	,	,	,
cord-021063-4y8m33ea	222	7	1991	1991	CD
cord-021063-4y8m33ea	222	8	;	;	:
cord-021063-4y8m33ea	222	9	Stribling	Stribling	NNP
cord-021063-4y8m33ea	222	10	et	et	FW
cord-021063-4y8m33ea	222	11	al	al	NNP
cord-021063-4y8m33ea	222	12	.	.	NNP
cord-021063-4y8m33ea	222	13	,	,	,
cord-021063-4y8m33ea	222	14	1992	1992	CD
cord-021063-4y8m33ea	222	15	)	)	-RRB-
cord-021063-4y8m33ea	222	16	.	.	.
cord-021063-4y8m33ea	223	1	Because	because	IN
cord-021063-4y8m33ea	223	2	liposomes	liposome	NNS
cord-021063-4y8m33ea	223	3	can	can	MD
cord-021063-4y8m33ea	223	4	not	not	RB
cord-021063-4y8m33ea	223	5	cross	cross	VB
cord-021063-4y8m33ea	223	6	the	the	DT
cord-021063-4y8m33ea	223	7	lung	lung	NN
cord-021063-4y8m33ea	223	8	epithelial	epithelial	JJ
cord-021063-4y8m33ea	223	9	barrier	barrier	NN
cord-021063-4y8m33ea	223	10	,	,	,
cord-021063-4y8m33ea	223	11	entrance	entrance	NN
cord-021063-4y8m33ea	223	12	into	into	IN
cord-021063-4y8m33ea	223	13	the	the	DT
cord-021063-4y8m33ea	223	14	general	general	JJ
cord-021063-4y8m33ea	223	15	circulation	circulation	NN
cord-021063-4y8m33ea	223	16	is	be	VBZ
cord-021063-4y8m33ea	223	17	prevented	prevent	VBN
cord-021063-4y8m33ea	223	18	.	.	.
cord-021063-4y8m33ea	224	1	Therefore	therefore	RB
cord-021063-4y8m33ea	224	2	,	,	,
cord-021063-4y8m33ea	224	3	it	-PRON-	PRP
cord-021063-4y8m33ea	224	4	is	be	VBZ
cord-021063-4y8m33ea	224	5	anticipated	anticipate	VBN
cord-021063-4y8m33ea	224	6	that	that	IN
cord-021063-4y8m33ea	224	7	liposome	liposome	NN
cord-021063-4y8m33ea	224	8	vectors	vector	NNS
cord-021063-4y8m33ea	224	9	will	will	MD
cord-021063-4y8m33ea	224	10	transfect	transfect	VB
cord-021063-4y8m33ea	224	11	only	only	RB
cord-021063-4y8m33ea	224	12	those	those	DT
cord-021063-4y8m33ea	224	13	cells	cell	NNS
cord-021063-4y8m33ea	224	14	present	present	JJ
cord-021063-4y8m33ea	224	15	on	on	IN
cord-021063-4y8m33ea	224	16	the	the	DT
cord-021063-4y8m33ea	224	17	epithelial	epithelial	JJ
cord-021063-4y8m33ea	224	18	surface	surface	NN
cord-021063-4y8m33ea	224	19	.	.	.
cord-021063-4y8m33ea	225	1	Another	another	DT
cord-021063-4y8m33ea	225	2	site	site	NN
cord-021063-4y8m33ea	225	3	where	where	WRB
cord-021063-4y8m33ea	225	4	this	this	DT
cord-021063-4y8m33ea	225	5	approach	approach	NN
cord-021063-4y8m33ea	225	6	may	may	MD
cord-021063-4y8m33ea	225	7	be	be	VB
cord-021063-4y8m33ea	225	8	applicable	applicable	JJ
cord-021063-4y8m33ea	225	9	is	be	VBZ
cord-021063-4y8m33ea	225	10	in	in	IN
cord-021063-4y8m33ea	225	11	the	the	DT
cord-021063-4y8m33ea	225	12	central	central	JJ
cord-021063-4y8m33ea	225	13	nervous	nervous	JJ
cord-021063-4y8m33ea	225	14	system	system	NN
cord-021063-4y8m33ea	225	15	(	(	-LRB-
cord-021063-4y8m33ea	225	16	CNS	CNS	NNP
cord-021063-4y8m33ea	225	17	)	)	-RRB-
cord-021063-4y8m33ea	225	18	.	.	.
cord-021063-4y8m33ea	226	1	In	in	IN
cord-021063-4y8m33ea	226	2	the	the	DT
cord-021063-4y8m33ea	226	3	cerebral	cerebral	JJ
cord-021063-4y8m33ea	226	4	vasculature	vasculature	NN
cord-021063-4y8m33ea	226	5	,	,	,
cord-021063-4y8m33ea	226	6	blood	blood	NN
cord-021063-4y8m33ea	226	7	is	be	VBZ
cord-021063-4y8m33ea	226	8	separated	separate	VBN
cord-021063-4y8m33ea	226	9	from	from	IN
cord-021063-4y8m33ea	226	10	the	the	DT
cord-021063-4y8m33ea	226	11	CNS	CNS	NNP
cord-021063-4y8m33ea	226	12	by	by	IN
cord-021063-4y8m33ea	226	13	the	the	DT
cord-021063-4y8m33ea	226	14	blood	blood	NN
cord-021063-4y8m33ea	226	15	-	-	HYPH
cord-021063-4y8m33ea	226	16	brain	brain	NN
cord-021063-4y8m33ea	226	17	barrier	barrier	NN
cord-021063-4y8m33ea	226	18	,	,	,
cord-021063-4y8m33ea	226	19	which	which	WDT
cord-021063-4y8m33ea	226	20	would	would	MD
cord-021063-4y8m33ea	226	21	normally	normally	RB
cord-021063-4y8m33ea	226	22	be	be	VB
cord-021063-4y8m33ea	226	23	completely	completely	RB
cord-021063-4y8m33ea	226	24	impermeable	impermeable	JJ
cord-021063-4y8m33ea	226	25	to	to	IN
cord-021063-4y8m33ea	226	26	any	any	DT
cord-021063-4y8m33ea	226	27	liposomes	liposome	NNS
cord-021063-4y8m33ea	226	28	.	.	.
cord-021063-4y8m33ea	227	1	However	however	RB
cord-021063-4y8m33ea	227	2	,	,	,
cord-021063-4y8m33ea	227	3	if	if	IN
cord-021063-4y8m33ea	227	4	the	the	DT
cord-021063-4y8m33ea	227	5	cerebral	cerebral	JJ
cord-021063-4y8m33ea	227	6	vasculature	vasculature	NN
cord-021063-4y8m33ea	227	7	is	be	VBZ
cord-021063-4y8m33ea	227	8	isolated	isolate	VBN
cord-021063-4y8m33ea	227	9	,	,	,
cord-021063-4y8m33ea	227	10	and	and	CC
cord-021063-4y8m33ea	227	11	perfused	perfuse	VBN
cord-021063-4y8m33ea	227	12	with	with	IN
cord-021063-4y8m33ea	227	13	a	a	DT
cord-021063-4y8m33ea	227	14	hyperosmotic	hyperosmotic	JJ
cord-021063-4y8m33ea	227	15	solution	solution	NN
cord-021063-4y8m33ea	227	16	,	,	,
cord-021063-4y8m33ea	227	17	the	the	DT
cord-021063-4y8m33ea	227	18	blood	blood	NN
cord-021063-4y8m33ea	227	19	-	-	HYPH
cord-021063-4y8m33ea	227	20	brain	brain	NN
cord-021063-4y8m33ea	227	21	barrier	barrier	NN
cord-021063-4y8m33ea	227	22	is	be	VBZ
cord-021063-4y8m33ea	227	23	damaged	damage	VBN
cord-021063-4y8m33ea	227	24	,	,	,
cord-021063-4y8m33ea	227	25	leaving	leave	VBG
cord-021063-4y8m33ea	227	26	holes	hole	NNS
cord-021063-4y8m33ea	227	27	large	large	JJ
cord-021063-4y8m33ea	227	28	enough	enough	RB
cord-021063-4y8m33ea	227	29	to	to	TO
cord-021063-4y8m33ea	227	30	admit	admit	VB
cord-021063-4y8m33ea	227	31	liposomes	liposome	NNS
cord-021063-4y8m33ea	227	32	into	into	IN
cord-021063-4y8m33ea	227	33	the	the	DT
cord-021063-4y8m33ea	227	34	CNS	CNS	NNP
cord-021063-4y8m33ea	227	35	(	(	-LRB-
cord-021063-4y8m33ea	227	36	Johansson	Johansson	NNP
cord-021063-4y8m33ea	227	37	,	,	,
cord-021063-4y8m33ea	227	38	1992	1992	CD
cord-021063-4y8m33ea	227	39	)	)	-RRB-
cord-021063-4y8m33ea	227	40	.	.	.
cord-021063-4y8m33ea	228	1	The	the	DT
cord-021063-4y8m33ea	228	2	blood	blood	NN
cord-021063-4y8m33ea	228	3	-	-	HYPH
cord-021063-4y8m33ea	228	4	brain	brain	NN
cord-021063-4y8m33ea	228	5	barrier	barrier	NN
cord-021063-4y8m33ea	228	6	reconstitutes	reconstitute	VBZ
cord-021063-4y8m33ea	228	7	itself	-PRON-	PRP
cord-021063-4y8m33ea	228	8	shortly	shortly	RB
cord-021063-4y8m33ea	228	9	thereafter	thereafter	RB
cord-021063-4y8m33ea	228	10	,	,	,
cord-021063-4y8m33ea	228	11	leaving	leave	VBG
cord-021063-4y8m33ea	228	12	the	the	DT
cord-021063-4y8m33ea	228	13	liposomes	liposome	NNS
cord-021063-4y8m33ea	228	14	trapped	trap	VBN
cord-021063-4y8m33ea	228	15	within	within	IN
cord-021063-4y8m33ea	228	16	the	the	DT
cord-021063-4y8m33ea	228	17	CNS	CNS	NNP
cord-021063-4y8m33ea	228	18	and	and	CC
cord-021063-4y8m33ea	228	19	unable	unable	JJ
cord-021063-4y8m33ea	228	20	to	to	TO
cord-021063-4y8m33ea	228	21	interact	interact	VB
cord-021063-4y8m33ea	228	22	with	with	IN
cord-021063-4y8m33ea	228	23	any	any	DT
cord-021063-4y8m33ea	228	24	other	other	JJ
cord-021063-4y8m33ea	228	25	cells	cell	NNS
cord-021063-4y8m33ea	228	26	.	.	.
cord-021063-4y8m33ea	229	1	May	May	MD
cord-021063-4y8m33ea	229	2	not	not	RB
cord-021063-4y8m33ea	229	3	be	be	VB
cord-021063-4y8m33ea	229	4	specific	specific	JJ
cord-021063-4y8m33ea	229	5	enough	enough	JJ
cord-021063-4y8m33ea	229	6	Second	second	JJ
cord-021063-4y8m33ea	229	7	,	,	,
cord-021063-4y8m33ea	229	8	the	the	DT
cord-021063-4y8m33ea	229	9	lipid	lipid	NN
cord-021063-4y8m33ea	229	10	composition	composition	NN
cord-021063-4y8m33ea	229	11	of	of	IN
cord-021063-4y8m33ea	229	12	the	the	DT
cord-021063-4y8m33ea	229	13	vesicles	vesicle	NNS
cord-021063-4y8m33ea	229	14	can	can	MD
cord-021063-4y8m33ea	229	15	be	be	VB
cord-021063-4y8m33ea	229	16	adjusted	adjust	VBN
cord-021063-4y8m33ea	229	17	to	to	TO
cord-021063-4y8m33ea	229	18	cause	cause	VB
cord-021063-4y8m33ea	229	19	them	-PRON-	PRP
cord-021063-4y8m33ea	229	20	to	to	TO
cord-021063-4y8m33ea	229	21	be	be	VB
cord-021063-4y8m33ea	229	22	taken	take	VBN
cord-021063-4y8m33ea	229	23	up	up	RP
cord-021063-4y8m33ea	229	24	preferentially	preferentially	RB
cord-021063-4y8m33ea	229	25	by	by	IN
cord-021063-4y8m33ea	229	26	some	some	DT
cord-021063-4y8m33ea	229	27	types	type	NNS
cord-021063-4y8m33ea	229	28	of	of	IN
cord-021063-4y8m33ea	229	29	cells	cell	NNS
cord-021063-4y8m33ea	229	30	.	.	.
cord-021063-4y8m33ea	230	1	For	for	IN
cord-021063-4y8m33ea	230	2	example	example	NN
cord-021063-4y8m33ea	230	3	,	,	,
cord-021063-4y8m33ea	230	4	macrophages	macrophage	NNS
cord-021063-4y8m33ea	230	5	have	have	VBP
cord-021063-4y8m33ea	230	6	a	a	DT
cord-021063-4y8m33ea	230	7	very	very	RB
cord-021063-4y8m33ea	230	8	high	high	JJ
cord-021063-4y8m33ea	230	9	affinity	affinity	NN
cord-021063-4y8m33ea	230	10	for	for	IN
cord-021063-4y8m33ea	230	11	liposomes	liposome	NNS
cord-021063-4y8m33ea	230	12	containing	contain	VBG
cord-021063-4y8m33ea	230	13	(	(	-LRB-
cord-021063-4y8m33ea	230	14	PS	PS	NNP
cord-021063-4y8m33ea	230	15	)	)	-RRB-
cord-021063-4y8m33ea	230	16	on	on	IN
cord-021063-4y8m33ea	230	17	their	-PRON-	PRP$
cord-021063-4y8m33ea	230	18	surface	surface	NN
cord-021063-4y8m33ea	231	1	(	(	-LRB-
cord-021063-4y8m33ea	231	2	Lee	Lee	NNP
cord-021063-4y8m33ea	231	3	et	et	NNP
cord-021063-4y8m33ea	231	4	al	al	NNP
cord-021063-4y8m33ea	231	5	.	.	NNP
cord-021063-4y8m33ea	231	6	,	,	,
cord-021063-4y8m33ea	231	7	1992	1992	CD
cord-021063-4y8m33ea	231	8	)	)	-RRB-
cord-021063-4y8m33ea	231	9	.	.	.
cord-021063-4y8m33ea	232	1	Consequently	consequently	RB
cord-021063-4y8m33ea	232	2	,	,	,
cord-021063-4y8m33ea	232	3	liposomes	liposome	NNS
cord-021063-4y8m33ea	232	4	containing	contain	VBG
cord-021063-4y8m33ea	232	5	a	a	DT
cord-021063-4y8m33ea	232	6	substantial	substantial	JJ
cord-021063-4y8m33ea	232	7	fraction	fraction	NN
cord-021063-4y8m33ea	232	8	of	of	IN
cord-021063-4y8m33ea	232	9	PS	PS	NNP
cord-021063-4y8m33ea	232	10	are	be	VBP
cord-021063-4y8m33ea	232	11	quickly	quickly	RB
cord-021063-4y8m33ea	232	12	cleared	clear	VBN
cord-021063-4y8m33ea	232	13	from	from	IN
cord-021063-4y8m33ea	232	14	the	the	DT
cord-021063-4y8m33ea	232	15	circulation	circulation	NN
cord-021063-4y8m33ea	232	16	by	by	IN
cord-021063-4y8m33ea	232	17	macrophages	macrophage	NNS
cord-021063-4y8m33ea	232	18	(	(	-LRB-
cord-021063-4y8m33ea	232	19	Allen	Allen	NNP
cord-021063-4y8m33ea	232	20	et	et	NNP
cord-021063-4y8m33ea	232	21	al	al	NNP
cord-021063-4y8m33ea	232	22	.	.	NNP
cord-021063-4y8m33ea	232	23	,	,	,
cord-021063-4y8m33ea	232	24	1989	1989	CD
cord-021063-4y8m33ea	232	25	)	)	-RRB-
cord-021063-4y8m33ea	232	26	.	.	.
cord-021063-4y8m33ea	233	1	A	a	DT
cord-021063-4y8m33ea	233	2	similar	similar	JJ
cord-021063-4y8m33ea	233	3	targeting	target	VBG
cord-021063-4y8m33ea	233	4	strategy	strategy	NN
cord-021063-4y8m33ea	233	5	uses	use	VBZ
cord-021063-4y8m33ea	233	6	lactosylceramide	lactosylceramide	NN
cord-021063-4y8m33ea	233	7	as	as	IN
cord-021063-4y8m33ea	233	8	a	a	DT
cord-021063-4y8m33ea	233	9	membrane	membrane	NN
cord-021063-4y8m33ea	233	10	component	component	NN
cord-021063-4y8m33ea	233	11	.	.	.
cord-021063-4y8m33ea	234	1	This	this	DT
cord-021063-4y8m33ea	234	2	lipid	lipid	NN
cord-021063-4y8m33ea	234	3	binds	bind	VBZ
cord-021063-4y8m33ea	234	4	to	to	IN
cord-021063-4y8m33ea	234	5	the	the	DT
cord-021063-4y8m33ea	234	6	asialoglycoprotein	asialoglycoprotein	NNP
cord-021063-4y8m33ea	234	7	receptors	receptor	NNS
cord-021063-4y8m33ea	234	8	found	find	VBN
cord-021063-4y8m33ea	234	9	on	on	IN
cord-021063-4y8m33ea	234	10	hepatocytes	hepatocyte	NNS
cord-021063-4y8m33ea	234	11	,	,	,
cord-021063-4y8m33ea	234	12	causing	cause	VBG
cord-021063-4y8m33ea	234	13	these	these	DT
cord-021063-4y8m33ea	234	14	cells	cell	NNS
cord-021063-4y8m33ea	234	15	to	to	TO
cord-021063-4y8m33ea	234	16	be	be	VB
cord-021063-4y8m33ea	234	17	preferentially	preferentially	RB
cord-021063-4y8m33ea	234	18	targeted	target	VBN
cord-021063-4y8m33ea	234	19	by	by	IN
cord-021063-4y8m33ea	234	20	the	the	DT
cord-021063-4y8m33ea	234	21	vesicles	vesicle	NNS
cord-021063-4y8m33ea	234	22	(	(	-LRB-
cord-021063-4y8m33ea	234	23	Grosse	Grosse	NNP
cord-021063-4y8m33ea	234	24	et	et	FW
cord-021063-4y8m33ea	234	25	al	al	NNP
cord-021063-4y8m33ea	234	26	.	.	NNP
cord-021063-4y8m33ea	234	27	,	,	,
cord-021063-4y8m33ea	234	28	1984	1984	CD
cord-021063-4y8m33ea	234	29	)	)	-RRB-
cord-021063-4y8m33ea	234	30	.	.	.
cord-021063-4y8m33ea	235	1	The	the	DT
cord-021063-4y8m33ea	235	2	final	final	JJ
cord-021063-4y8m33ea	235	3	method	method	NN
cord-021063-4y8m33ea	235	4	involves	involve	VBZ
cord-021063-4y8m33ea	235	5	the	the	DT
cord-021063-4y8m33ea	235	6	addition	addition	NN
cord-021063-4y8m33ea	235	7	of	of	IN
cord-021063-4y8m33ea	235	8	antibodies	antibody	NNS
cord-021063-4y8m33ea	235	9	or	or	CC
cord-021063-4y8m33ea	235	10	other	other	JJ
cord-021063-4y8m33ea	235	11	proteins	protein	NNS
cord-021063-4y8m33ea	235	12	to	to	IN
cord-021063-4y8m33ea	235	13	the	the	DT
cord-021063-4y8m33ea	235	14	surface	surface	NN
cord-021063-4y8m33ea	235	15	of	of	IN
cord-021063-4y8m33ea	235	16	the	the	DT
cord-021063-4y8m33ea	235	17	liposome	liposome	NN
cord-021063-4y8m33ea	235	18	after	after	IN
cord-021063-4y8m33ea	235	19	it	-PRON-	PRP
cord-021063-4y8m33ea	235	20	has	have	VBZ
cord-021063-4y8m33ea	235	21	been	be	VBN
cord-021063-4y8m33ea	235	22	formed	form	VBN
cord-021063-4y8m33ea	235	23	(	(	-LRB-
cord-021063-4y8m33ea	235	24	Loughrey	Loughrey	NNP
cord-021063-4y8m33ea	235	25	et	et	NNP
cord-021063-4y8m33ea	235	26	al	al	NNP
cord-021063-4y8m33ea	235	27	.	.	NNP
cord-021063-4y8m33ea	235	28	,	,	,
cord-021063-4y8m33ea	235	29	1990	1990	CD
cord-021063-4y8m33ea	235	30	)	)	-RRB-
cord-021063-4y8m33ea	235	31	.	.	.
cord-021063-4y8m33ea	236	1	These	these	DT
cord-021063-4y8m33ea	236	2	"	"	``
cord-021063-4y8m33ea	236	3	proteoliposomes	proteoliposome	NNS
cord-021063-4y8m33ea	236	4	"	"	''
cord-021063-4y8m33ea	236	5	are	be	VBP
cord-021063-4y8m33ea	236	6	taken	take	VBN
cord-021063-4y8m33ea	236	7	up	up	RP
cord-021063-4y8m33ea	236	8	by	by	IN
cord-021063-4y8m33ea	236	9	cells	cell	NNS
cord-021063-4y8m33ea	236	10	to	to	TO
cord-021063-4y8m33ea	236	11	which	which	WDT
cord-021063-4y8m33ea	236	12	they	-PRON-	PRP
cord-021063-4y8m33ea	236	13	can	can	MD
cord-021063-4y8m33ea	236	14	bind	bind	VB
cord-021063-4y8m33ea	236	15	.	.	.
cord-021063-4y8m33ea	237	1	Table	table	NN
cord-021063-4y8m33ea	237	2	5	5	CD
cord-021063-4y8m33ea	237	3	gives	give	VBZ
cord-021063-4y8m33ea	237	4	a	a	DT
cord-021063-4y8m33ea	237	5	representative	representative	JJ
cord-021063-4y8m33ea	237	6	list	list	NN
cord-021063-4y8m33ea	237	7	of	of	IN
cord-021063-4y8m33ea	237	8	proteins	protein	NNS
cord-021063-4y8m33ea	237	9	that	that	WDT
cord-021063-4y8m33ea	237	10	have	have	VBP
cord-021063-4y8m33ea	237	11	been	be	VBN
cord-021063-4y8m33ea	237	12	used	use	VBN
cord-021063-4y8m33ea	237	13	to	to	TO
cord-021063-4y8m33ea	237	14	target	target	VB
cord-021063-4y8m33ea	237	15	liposomes	liposome	NNS
cord-021063-4y8m33ea	237	16	to	to	IN
cord-021063-4y8m33ea	237	17	cells	cell	NNS
cord-021063-4y8m33ea	237	18	.	.	.
cord-021063-4y8m33ea	238	1	Many	many	JJ
cord-021063-4y8m33ea	238	2	proteins	protein	NNS
cord-021063-4y8m33ea	238	3	can	can	MD
cord-021063-4y8m33ea	238	4	be	be	VB
cord-021063-4y8m33ea	238	5	modified	modify	VBN
cord-021063-4y8m33ea	238	6	by	by	IN
cord-021063-4y8m33ea	238	7	the	the	DT
cord-021063-4y8m33ea	238	8	addition	addition	NN
cord-021063-4y8m33ea	238	9	of	of	IN
cord-021063-4y8m33ea	238	10	phospholipid	phospholipid	NN
cord-021063-4y8m33ea	238	11	molecules	molecule	NNS
cord-021063-4y8m33ea	238	12	or	or	CC
cord-021063-4y8m33ea	238	13	acyl	acyl	NN
cord-021063-4y8m33ea	238	14	chains	chain	NNS
cord-021063-4y8m33ea	238	15	and	and	CC
cord-021063-4y8m33ea	238	16	retain	retain	VB
cord-021063-4y8m33ea	238	17	their	-PRON-	PRP$
cord-021063-4y8m33ea	238	18	native	native	JJ
cord-021063-4y8m33ea	238	19	function	function	NN
cord-021063-4y8m33ea	238	20	.	.	.
cord-021063-4y8m33ea	239	1	These	these	DT
cord-021063-4y8m33ea	239	2	modified	modify	VBN
cord-021063-4y8m33ea	239	3	proteins	protein	NNS
cord-021063-4y8m33ea	239	4	can	can	MD
cord-021063-4y8m33ea	239	5	be	be	VB
cord-021063-4y8m33ea	239	6	coupled	couple	VBN
cord-021063-4y8m33ea	239	7	to	to	IN
cord-021063-4y8m33ea	239	8	a	a	DT
cord-021063-4y8m33ea	239	9	preexisting	preexist	VBG
cord-021063-4y8m33ea	239	10	bilayer	bilayer	NN
cord-021063-4y8m33ea	239	11	,	,	,
cord-021063-4y8m33ea	239	12	endowing	endow	VBG
cord-021063-4y8m33ea	239	13	the	the	DT
cord-021063-4y8m33ea	239	14	liposome	liposome	NN
cord-021063-4y8m33ea	239	15	with	with	IN
cord-021063-4y8m33ea	239	16	their	-PRON-	PRP$
cord-021063-4y8m33ea	239	17	binding	bind	VBG
cord-021063-4y8m33ea	239	18	properties	property	NNS
cord-021063-4y8m33ea	239	19	.	.	.
cord-021063-4y8m33ea	240	1	All	all	DT
cord-021063-4y8m33ea	240	2	three	three	CD
cord-021063-4y8m33ea	240	3	methods	method	NNS
cord-021063-4y8m33ea	240	4	described	describe	VBN
cord-021063-4y8m33ea	240	5	above	above	RB
cord-021063-4y8m33ea	240	6	have	have	VBP
cord-021063-4y8m33ea	240	7	been	be	VBN
cord-021063-4y8m33ea	240	8	used	use	VBN
cord-021063-4y8m33ea	240	9	successfully	successfully	RB
cord-021063-4y8m33ea	240	10	to	to	TO
cord-021063-4y8m33ea	240	11	direct	direct	VB
cord-021063-4y8m33ea	240	12	the	the	DT
cord-021063-4y8m33ea	240	13	uptake	uptake	NN
cord-021063-4y8m33ea	240	14	of	of	IN
cord-021063-4y8m33ea	240	15	liposomes	liposome	NNS
cord-021063-4y8m33ea	240	16	to	to	IN
cord-021063-4y8m33ea	240	17	specific	specific	JJ
cord-021063-4y8m33ea	240	18	cell	cell	NN
cord-021063-4y8m33ea	240	19	types	type	NNS
cord-021063-4y8m33ea	240	20	.	.	.
cord-021063-4y8m33ea	241	1	The	the	DT
cord-021063-4y8m33ea	241	2	methods	method	NNS
cord-021063-4y8m33ea	241	3	are	be	VBP
cord-021063-4y8m33ea	241	4	not	not	RB
cord-021063-4y8m33ea	241	5	mutually	mutually	RB
cord-021063-4y8m33ea	241	6	exclusive	exclusive	JJ
cord-021063-4y8m33ea	241	7	and	and	CC
cord-021063-4y8m33ea	241	8	,	,	,
cord-021063-4y8m33ea	241	9	in	in	IN
cord-021063-4y8m33ea	241	10	principle	principle	NN
cord-021063-4y8m33ea	241	11	,	,	,
cord-021063-4y8m33ea	241	12	it	-PRON-	PRP
cord-021063-4y8m33ea	241	13	should	should	MD
cord-021063-4y8m33ea	241	14	be	be	VB
cord-021063-4y8m33ea	241	15	possible	possible	JJ
cord-021063-4y8m33ea	241	16	to	to	TO
cord-021063-4y8m33ea	241	17	combine	combine	VB
cord-021063-4y8m33ea	241	18	them	-PRON-	PRP
cord-021063-4y8m33ea	241	19	for	for	IN
cord-021063-4y8m33ea	241	20	more	more	RBR
cord-021063-4y8m33ea	241	21	specific	specific	JJ
cord-021063-4y8m33ea	241	22	targeting	targeting	NN
cord-021063-4y8m33ea	241	23	.	.	.
cord-021063-4y8m33ea	242	1	The	the	DT
cord-021063-4y8m33ea	242	2	central	central	JJ
cord-021063-4y8m33ea	242	3	problem	problem	NN
cord-021063-4y8m33ea	242	4	of	of	IN
cord-021063-4y8m33ea	242	5	any	any	DT
cord-021063-4y8m33ea	242	6	liposome	liposome	NN
cord-021063-4y8m33ea	242	7	-	-	HYPH
cord-021063-4y8m33ea	242	8	based	base	VBN
cord-021063-4y8m33ea	242	9	gene	gene	NN
cord-021063-4y8m33ea	242	10	therapy	therapy	NN
cord-021063-4y8m33ea	242	11	system	system	NN
cord-021063-4y8m33ea	242	12	is	be	VBZ
cord-021063-4y8m33ea	242	13	that	that	IN
cord-021063-4y8m33ea	242	14	DNA	DNA	NNP
cord-021063-4y8m33ea	242	15	must	must	MD
cord-021063-4y8m33ea	242	16	be	be	VB
cord-021063-4y8m33ea	242	17	introduced	introduce	VBN
cord-021063-4y8m33ea	242	18	into	into	IN
cord-021063-4y8m33ea	242	19	the	the	DT
cord-021063-4y8m33ea	242	20	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	242	21	of	of	IN
cord-021063-4y8m33ea	242	22	the	the	DT
cord-021063-4y8m33ea	242	23	target	target	NN
cord-021063-4y8m33ea	242	24	cell	cell	NN
cord-021063-4y8m33ea	242	25	.	.	.
cord-021063-4y8m33ea	243	1	When	when	WRB
cord-021063-4y8m33ea	243	2	DNA	dna	NN
cord-021063-4y8m33ea	243	3	-	-	HYPH
cord-021063-4y8m33ea	243	4	loaded	load	VBN
cord-021063-4y8m33ea	243	5	liposomes	liposome	NNS
cord-021063-4y8m33ea	243	6	are	be	VBP
cord-021063-4y8m33ea	243	7	added	add	VBN
cord-021063-4y8m33ea	243	8	to	to	IN
cord-021063-4y8m33ea	243	9	cells	cell	NNS
cord-021063-4y8m33ea	243	10	they	-PRON-	PRP
cord-021063-4y8m33ea	243	11	are	be	VBP
cord-021063-4y8m33ea	243	12	endocytosed	endocytose	VBN
cord-021063-4y8m33ea	243	13	,	,	,
cord-021063-4y8m33ea	243	14	and	and	CC
cord-021063-4y8m33ea	243	15	most	most	JJS
cord-021063-4y8m33ea	243	16	are	be	VBP
cord-021063-4y8m33ea	243	17	carried	carry	VBN
cord-021063-4y8m33ea	243	18	to	to	IN
cord-021063-4y8m33ea	243	19	lysosomes	lysosome	NNS
cord-021063-4y8m33ea	243	20	where	where	WRB
cord-021063-4y8m33ea	243	21	they	-PRON-	PRP
cord-021063-4y8m33ea	243	22	are	be	VBP
cord-021063-4y8m33ea	243	23	degraded	degrade	VBN
cord-021063-4y8m33ea	243	24	by	by	IN
cord-021063-4y8m33ea	243	25	digestive	digestive	JJ
cord-021063-4y8m33ea	243	26	enzymes	enzyme	NNS
cord-021063-4y8m33ea	243	27	.	.	.
cord-021063-4y8m33ea	244	1	Somehow	somehow	RB
cord-021063-4y8m33ea	244	2	,	,	,
cord-021063-4y8m33ea	244	3	a	a	DT
cord-021063-4y8m33ea	244	4	small	small	JJ
cord-021063-4y8m33ea	244	5	fraction	fraction	NN
cord-021063-4y8m33ea	244	6	of	of	IN
cord-021063-4y8m33ea	244	7	encapsulated	encapsulate	VBN
cord-021063-4y8m33ea	244	8	DNA	dna	NN
cord-021063-4y8m33ea	244	9	escapes	escape	VBZ
cord-021063-4y8m33ea	244	10	this	this	DT
cord-021063-4y8m33ea	244	11	pathway	pathway	NN
cord-021063-4y8m33ea	244	12	,	,	,
cord-021063-4y8m33ea	244	13	enters	enter	VBZ
cord-021063-4y8m33ea	244	14	the	the	DT
cord-021063-4y8m33ea	244	15	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	244	16	,	,	,
cord-021063-4y8m33ea	244	17	and	and	CC
cord-021063-4y8m33ea	244	18	is	be	VBZ
cord-021063-4y8m33ea	244	19	expressed	express	VBN
cord-021063-4y8m33ea	244	20	by	by	IN
cord-021063-4y8m33ea	244	21	the	the	DT
cord-021063-4y8m33ea	244	22	cells	cell	NNS
cord-021063-4y8m33ea	244	23	(	(	-LRB-
cord-021063-4y8m33ea	244	24	Straubinger	Straubinger	NNP
cord-021063-4y8m33ea	244	25	et	et	NNP
cord-021063-4y8m33ea	244	26	al	al	NNP
cord-021063-4y8m33ea	244	27	.	.	NNP
cord-021063-4y8m33ea	244	28	,	,	,
cord-021063-4y8m33ea	244	29	1990	1990	CD
cord-021063-4y8m33ea	244	30	)	)	-RRB-
cord-021063-4y8m33ea	244	31	.	.	.
cord-021063-4y8m33ea	245	1	Several	several	JJ
cord-021063-4y8m33ea	245	2	methods	method	NNS
cord-021063-4y8m33ea	245	3	have	have	VBP
cord-021063-4y8m33ea	245	4	been	be	VBN
cord-021063-4y8m33ea	245	5	developed	develop	VBN
cord-021063-4y8m33ea	245	6	to	to	TO
cord-021063-4y8m33ea	245	7	increase	increase	VB
cord-021063-4y8m33ea	245	8	the	the	DT
cord-021063-4y8m33ea	245	9	proportion	proportion	NN
cord-021063-4y8m33ea	245	10	of	of	IN
cord-021063-4y8m33ea	245	11	DNA	dna	NN
cord-021063-4y8m33ea	245	12	delivered	deliver	VBN
cord-021063-4y8m33ea	245	13	to	to	IN
cord-021063-4y8m33ea	245	14	the	the	DT
cord-021063-4y8m33ea	245	15	cytosol	cytosol	NN
cord-021063-4y8m33ea	245	16	.	.	.
cord-021063-4y8m33ea	246	1	Two	two	CD
cord-021063-4y8m33ea	246	2	general	general	JJ
cord-021063-4y8m33ea	246	3	approaches	approach	NNS
cord-021063-4y8m33ea	246	4	that	that	WDT
cord-021063-4y8m33ea	246	5	have	have	VBP
cord-021063-4y8m33ea	246	6	been	be	VBN
cord-021063-4y8m33ea	246	7	used	use	VBN
cord-021063-4y8m33ea	246	8	successfully	successfully	RB
cord-021063-4y8m33ea	246	9	are	be	VBP
cord-021063-4y8m33ea	246	10	:	:	:
cord-021063-4y8m33ea	246	11	(	(	-LRB-
cord-021063-4y8m33ea	246	12	i	i	NN
cord-021063-4y8m33ea	246	13	)	)	-RRB-
cord-021063-4y8m33ea	246	14	bypassing	bypass	VBG
cord-021063-4y8m33ea	246	15	the	the	DT
cord-021063-4y8m33ea	246	16	endocytic	endocytic	JJ
cord-021063-4y8m33ea	246	17	pathway	pathway	NN
cord-021063-4y8m33ea	246	18	completely	completely	RB
cord-021063-4y8m33ea	246	19	,	,	,
cord-021063-4y8m33ea	246	20	and	and	CC
cord-021063-4y8m33ea	246	21	(	(	-LRB-
cord-021063-4y8m33ea	246	22	ii	ii	NN
cord-021063-4y8m33ea	246	23	)	)	-RRB-
cord-021063-4y8m33ea	246	24	using	use	VBG
cord-021063-4y8m33ea	246	25	the	the	DT
cord-021063-4y8m33ea	246	26	initial	initial	JJ
cord-021063-4y8m33ea	246	27	stages	stage	NNS
cord-021063-4y8m33ea	246	28	of	of	IN
cord-021063-4y8m33ea	246	29	the	the	DT
cord-021063-4y8m33ea	246	30	endocytic	endocytic	JJ
cord-021063-4y8m33ea	246	31	pathway	pathway	NN
cord-021063-4y8m33ea	246	32	but	but	CC
cord-021063-4y8m33ea	246	33	escaping	escape	VBG
cord-021063-4y8m33ea	246	34	the	the	DT
cord-021063-4y8m33ea	246	35	pathway	pathway	NN
cord-021063-4y8m33ea	246	36	before	before	IN
cord-021063-4y8m33ea	246	37	delivery	delivery	NN
cord-021063-4y8m33ea	246	38	of	of	IN
cord-021063-4y8m33ea	246	39	the	the	DT
cord-021063-4y8m33ea	246	40	DNA	dna	NN
cord-021063-4y8m33ea	246	41	to	to	IN
cord-021063-4y8m33ea	246	42	lysosomes	lysosome	NNS
cord-021063-4y8m33ea	246	43	.	.	.
cord-021063-4y8m33ea	247	1	The	the	DT
cord-021063-4y8m33ea	247	2	endocytic	endocytic	JJ
cord-021063-4y8m33ea	247	3	pathway	pathway	NN
cord-021063-4y8m33ea	247	4	of	of	IN
cord-021063-4y8m33ea	247	5	liposomal	liposomal	JJ
cord-021063-4y8m33ea	247	6	DNA	dna	NN
cord-021063-4y8m33ea	247	7	entry	entry	NN
cord-021063-4y8m33ea	247	8	into	into	IN
cord-021063-4y8m33ea	247	9	the	the	DT
cord-021063-4y8m33ea	247	10	cell	cell	NN
cord-021063-4y8m33ea	247	11	is	be	VBZ
cord-021063-4y8m33ea	247	12	bypassed	bypass	VBN
cord-021063-4y8m33ea	247	13	when	when	WRB
cord-021063-4y8m33ea	247	14	liposomes	liposome	NNS
cord-021063-4y8m33ea	247	15	are	be	VBP
cord-021063-4y8m33ea	247	16	fused	fuse	VBN
cord-021063-4y8m33ea	247	17	directly	directly	RB
cord-021063-4y8m33ea	247	18	to	to	IN
cord-021063-4y8m33ea	247	19	the	the	DT
cord-021063-4y8m33ea	247	20	plasma	plasma	NN
cord-021063-4y8m33ea	247	21	membrane	membrane	NN
cord-021063-4y8m33ea	247	22	of	of	IN
cord-021063-4y8m33ea	247	23	the	the	DT
cord-021063-4y8m33ea	247	24	cell	cell	NN
cord-021063-4y8m33ea	247	25	.	.	.
cord-021063-4y8m33ea	248	1	This	this	DT
cord-021063-4y8m33ea	248	2	has	have	VBZ
cord-021063-4y8m33ea	248	3	been	be	VBN
cord-021063-4y8m33ea	248	4	accomplished	accomplish	VBN
cord-021063-4y8m33ea	248	5	using	use	VBG
cord-021063-4y8m33ea	248	6	virosomes	virosome	NNS
cord-021063-4y8m33ea	248	7	containing	contain	VBG
cord-021063-4y8m33ea	248	8	a	a	DT
cord-021063-4y8m33ea	248	9	constitutively	constitutively	RB
cord-021063-4y8m33ea	248	10	active	active	JJ
cord-021063-4y8m33ea	248	11	viral	viral	JJ
cord-021063-4y8m33ea	248	12	fusion	fusion	NN
cord-021063-4y8m33ea	248	13	protein	protein	NN
cord-021063-4y8m33ea	248	14	(	(	-LRB-
cord-021063-4y8m33ea	248	15	Nakanishi	Nakanishi	NNP
cord-021063-4y8m33ea	248	16	et	et	NNP
cord-021063-4y8m33ea	248	17	al	al	NNP
cord-021063-4y8m33ea	248	18	.	.	NNP
cord-021063-4y8m33ea	248	19	,	,	,
cord-021063-4y8m33ea	248	20	1987	1987	CD
cord-021063-4y8m33ea	248	21	)	)	-RRB-
cord-021063-4y8m33ea	248	22	.	.	.
cord-021063-4y8m33ea	249	1	As	as	RB
cord-021063-4y8m33ea	249	2	soon	soon	RB
cord-021063-4y8m33ea	249	3	as	as	IN
cord-021063-4y8m33ea	249	4	the	the	DT
cord-021063-4y8m33ea	249	5	virosome	virosome	NN
cord-021063-4y8m33ea	249	6	binds	bind	VBZ
cord-021063-4y8m33ea	249	7	to	to	IN
cord-021063-4y8m33ea	249	8	the	the	DT
cord-021063-4y8m33ea	249	9	cell	cell	NN
cord-021063-4y8m33ea	249	10	surface	surface	NN
cord-021063-4y8m33ea	249	11	,	,	,
cord-021063-4y8m33ea	249	12	the	the	DT
cord-021063-4y8m33ea	249	13	fusion	fusion	NN
cord-021063-4y8m33ea	249	14	protein	protein	NN
cord-021063-4y8m33ea	249	15	acts	act	VBZ
cord-021063-4y8m33ea	249	16	to	to	TO
cord-021063-4y8m33ea	249	17	fuse	fuse	VB
cord-021063-4y8m33ea	249	18	the	the	DT
cord-021063-4y8m33ea	249	19	virosomal	virosomal	JJ
cord-021063-4y8m33ea	249	20	membrane	membrane	NN
cord-021063-4y8m33ea	249	21	with	with	IN
cord-021063-4y8m33ea	249	22	that	that	DT
cord-021063-4y8m33ea	249	23	of	of	IN
cord-021063-4y8m33ea	249	24	the	the	DT
cord-021063-4y8m33ea	249	25	cell	cell	NN
cord-021063-4y8m33ea	249	26	,	,	,
cord-021063-4y8m33ea	249	27	causing	cause	VBG
cord-021063-4y8m33ea	249	28	the	the	DT
cord-021063-4y8m33ea	249	29	vesicle	vesicle	NN
cord-021063-4y8m33ea	249	30	contents	content	NNS
cord-021063-4y8m33ea	249	31	to	to	TO
cord-021063-4y8m33ea	249	32	be	be	VB
cord-021063-4y8m33ea	249	33	introduced	introduce	VBN
cord-021063-4y8m33ea	249	34	directly	directly	RB
cord-021063-4y8m33ea	249	35	into	into	IN
cord-021063-4y8m33ea	249	36	the	the	DT
cord-021063-4y8m33ea	249	37	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	249	38	.	.	.
cord-021063-4y8m33ea	250	1	Some	some	DT
cord-021063-4y8m33ea	250	2	liposome	liposome	NN
cord-021063-4y8m33ea	250	3	delivery	delivery	NN
cord-021063-4y8m33ea	250	4	systems	system	NNS
cord-021063-4y8m33ea	250	5	use	use	VBP
cord-021063-4y8m33ea	250	6	the	the	DT
cord-021063-4y8m33ea	250	7	initial	initial	JJ
cord-021063-4y8m33ea	250	8	stages	stage	NNS
cord-021063-4y8m33ea	250	9	of	of	IN
cord-021063-4y8m33ea	250	10	the	the	DT
cord-021063-4y8m33ea	250	11	endocytic	endocytic	JJ
cord-021063-4y8m33ea	250	12	pathway	pathway	NN
cord-021063-4y8m33ea	250	13	in	in	IN
cord-021063-4y8m33ea	250	14	the	the	DT
cord-021063-4y8m33ea	250	15	delivery	delivery	NN
cord-021063-4y8m33ea	250	16	process	process	NN
cord-021063-4y8m33ea	250	17	.	.	.
cord-021063-4y8m33ea	251	1	This	this	DT
cord-021063-4y8m33ea	251	2	strategy	strategy	NN
cord-021063-4y8m33ea	251	3	takes	take	VBZ
cord-021063-4y8m33ea	251	4	advantage	advantage	NN
cord-021063-4y8m33ea	251	5	of	of	IN
cord-021063-4y8m33ea	251	6	the	the	DT
cord-021063-4y8m33ea	251	7	low	low	JJ
cord-021063-4y8m33ea	251	8	pH	ph	NN
cord-021063-4y8m33ea	251	9	of	of	IN
cord-021063-4y8m33ea	251	10	endosomes	endosome	NNS
cord-021063-4y8m33ea	251	11	,	,	,
cord-021063-4y8m33ea	251	12	which	which	WDT
cord-021063-4y8m33ea	251	13	can	can	MD
cord-021063-4y8m33ea	251	14	be	be	VB
cord-021063-4y8m33ea	251	15	used	use	VBN
cord-021063-4y8m33ea	251	16	to	to	TO
cord-021063-4y8m33ea	251	17	trigger	trigger	VB
cord-021063-4y8m33ea	251	18	fusion	fusion	NN
cord-021063-4y8m33ea	251	19	of	of	IN
cord-021063-4y8m33ea	251	20	liposomes	liposome	NNS
cord-021063-4y8m33ea	251	21	with	with	IN
cord-021063-4y8m33ea	251	22	the	the	DT
cord-021063-4y8m33ea	251	23	endosomal	endosomal	NNP
cord-021063-4y8m33ea	251	24	membrane	membrane	NN
cord-021063-4y8m33ea	251	25	.	.	.
cord-021063-4y8m33ea	252	1	pH	ph	JJ
cord-021063-4y8m33ea	252	2	-	-	HYPH
cord-021063-4y8m33ea	252	3	dependent	dependent	JJ
cord-021063-4y8m33ea	252	4	fusion	fusion	NN
cord-021063-4y8m33ea	252	5	of	of	IN
cord-021063-4y8m33ea	252	6	liposomes	liposome	NNS
cord-021063-4y8m33ea	252	7	with	with	IN
cord-021063-4y8m33ea	252	8	endosomes	endosome	NNS
cord-021063-4y8m33ea	252	9	has	have	VBZ
cord-021063-4y8m33ea	252	10	been	be	VBN
cord-021063-4y8m33ea	252	11	achieved	achieve	VBN
cord-021063-4y8m33ea	252	12	using	use	VBG
cord-021063-4y8m33ea	252	13	vesicles	vesicle	NNS
cord-021063-4y8m33ea	252	14	composed	compose	VBN
cord-021063-4y8m33ea	252	15	of	of	IN
cord-021063-4y8m33ea	252	16	phosphatidylethanolamine	phosphatidylethanolamine	JJ
cord-021063-4y8m33ea	252	17	(	(	-LRB-
cord-021063-4y8m33ea	252	18	PE	PE	NNP
cord-021063-4y8m33ea	252	19	)	)	-RRB-
cord-021063-4y8m33ea	252	20	and	and	CC
cord-021063-4y8m33ea	252	21	substances	substance	NNS
cord-021063-4y8m33ea	252	22	such	such	JJ
cord-021063-4y8m33ea	252	23	as	as	IN
cord-021063-4y8m33ea	252	24	dipalmitoylsuccinylglycerol	dipalmitoylsuccinylglycerol	NNP
cord-021063-4y8m33ea	252	25	that	that	WDT
cord-021063-4y8m33ea	252	26	leave	leave	VBP
cord-021063-4y8m33ea	252	27	the	the	DT
cord-021063-4y8m33ea	252	28	membrane	membrane	NN
cord-021063-4y8m33ea	252	29	at	at	IN
cord-021063-4y8m33ea	252	30	low	low	JJ
cord-021063-4y8m33ea	252	31	pH	ph	NN
cord-021063-4y8m33ea	253	1	(	(	-LRB-
cord-021063-4y8m33ea	253	2	Liu	Liu	NNP
cord-021063-4y8m33ea	253	3	and	and	CC
cord-021063-4y8m33ea	253	4	Huang	Huang	NNP
cord-021063-4y8m33ea	253	5	,	,	,
cord-021063-4y8m33ea	253	6	1990	1990	CD
cord-021063-4y8m33ea	253	7	)	)	-RRB-
cord-021063-4y8m33ea	253	8	.	.	.
cord-021063-4y8m33ea	254	1	As	as	IN
cord-021063-4y8m33ea	254	2	the	the	DT
cord-021063-4y8m33ea	254	3	pH	pH	NNP
cord-021063-4y8m33ea	254	4	is	be	VBZ
cord-021063-4y8m33ea	254	5	lowered	lower	VBN
cord-021063-4y8m33ea	254	6	in	in	IN
cord-021063-4y8m33ea	254	7	the	the	DT
cord-021063-4y8m33ea	254	8	endosome	endosome	NN
cord-021063-4y8m33ea	254	9	,	,	,
cord-021063-4y8m33ea	254	10	the	the	DT
cord-021063-4y8m33ea	254	11	effective	effective	JJ
cord-021063-4y8m33ea	254	12	concentration	concentration	NN
cord-021063-4y8m33ea	254	13	of	of	IN
cord-021063-4y8m33ea	254	14	PE	PE	NNP
cord-021063-4y8m33ea	254	15	rises	rise	NNS
cord-021063-4y8m33ea	254	16	.	.	.
cord-021063-4y8m33ea	255	1	When	when	WRB
cord-021063-4y8m33ea	255	2	the	the	DT
cord-021063-4y8m33ea	255	3	concentration	concentration	NN
cord-021063-4y8m33ea	255	4	rises	rise	VBZ
cord-021063-4y8m33ea	255	5	above	above	IN
cord-021063-4y8m33ea	255	6	60	60	CD
cord-021063-4y8m33ea	255	7	mole	mole	NN
cord-021063-4y8m33ea	255	8	percent	percent	NN
cord-021063-4y8m33ea	255	9	,	,	,
cord-021063-4y8m33ea	255	10	the	the	DT
cord-021063-4y8m33ea	255	11	vesicle	vesicle	NN
cord-021063-4y8m33ea	255	12	membrane	membrane	NN
cord-021063-4y8m33ea	255	13	can	can	MD
cord-021063-4y8m33ea	255	14	no	no	RB
cord-021063-4y8m33ea	255	15	longer	longer	RB
cord-021063-4y8m33ea	255	16	remain	remain	VB
cord-021063-4y8m33ea	255	17	as	as	IN
cord-021063-4y8m33ea	255	18	a	a	DT
cord-021063-4y8m33ea	255	19	bilayer	bilayer	NN
cord-021063-4y8m33ea	255	20	.	.	.
cord-021063-4y8m33ea	256	1	As	as	IN
cord-021063-4y8m33ea	256	2	it	-PRON-	PRP
cord-021063-4y8m33ea	256	3	converts	convert	VBZ
cord-021063-4y8m33ea	256	4	to	to	IN
cord-021063-4y8m33ea	256	5	the	the	DT
cord-021063-4y8m33ea	256	6	inverted	inverted	JJ
cord-021063-4y8m33ea	256	7	hexagonal	hexagonal	JJ
cord-021063-4y8m33ea	256	8	phase	phase	NN
cord-021063-4y8m33ea	256	9	,	,	,
cord-021063-4y8m33ea	256	10	it	-PRON-	PRP
cord-021063-4y8m33ea	256	11	destabilizes	destabilize	VBZ
cord-021063-4y8m33ea	256	12	the	the	DT
cord-021063-4y8m33ea	256	13	adjacent	adjacent	JJ
cord-021063-4y8m33ea	256	14	endosomal	endosomal	NNP
cord-021063-4y8m33ea	256	15	membrane	membrane	NN
cord-021063-4y8m33ea	256	16	,	,	,
cord-021063-4y8m33ea	256	17	and	and	CC
cord-021063-4y8m33ea	256	18	releases	release	VBZ
cord-021063-4y8m33ea	256	19	the	the	DT
cord-021063-4y8m33ea	256	20	liposomal	liposomal	JJ
cord-021063-4y8m33ea	256	21	contents	content	NNS
cord-021063-4y8m33ea	256	22	into	into	IN
cord-021063-4y8m33ea	256	23	the	the	DT
cord-021063-4y8m33ea	256	24	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	256	25	.	.	.
cord-021063-4y8m33ea	257	1	Similar	similar	JJ
cord-021063-4y8m33ea	257	2	results	result	NNS
cord-021063-4y8m33ea	257	3	can	can	MD
cord-021063-4y8m33ea	257	4	be	be	VB
cord-021063-4y8m33ea	257	5	seen	see	VBN
cord-021063-4y8m33ea	257	6	using	use	VBG
cord-021063-4y8m33ea	257	7	virosomes	virosomes	NN
cord-021063-4y8m33ea	257	8	containing	contain	VBG
cord-021063-4y8m33ea	257	9	pH	ph	JJ
cord-021063-4y8m33ea	257	10	-	-	HYPH
cord-021063-4y8m33ea	257	11	sensitive	sensitive	JJ
cord-021063-4y8m33ea	257	12	fusion	fusion	NN
cord-021063-4y8m33ea	257	13	proteins	protein	NNS
cord-021063-4y8m33ea	258	1	(	(	-LRB-
cord-021063-4y8m33ea	258	2	Gould	Gould	NNP
cord-021063-4y8m33ea	258	3	-	-	HYPH
cord-021063-4y8m33ea	258	4	Fogerite	Fogerite	NNP
cord-021063-4y8m33ea	258	5	et	et	NNP
cord-021063-4y8m33ea	258	6	al	al	NNP
cord-021063-4y8m33ea	258	7	.	.	NNP
cord-021063-4y8m33ea	258	8	,	,	,
cord-021063-4y8m33ea	258	9	1989	1989	CD
cord-021063-4y8m33ea	258	10	;	;	:
cord-021063-4y8m33ea	258	11	Lapidot	Lapidot	NNP
cord-021063-4y8m33ea	258	12	and	and	CC
cord-021063-4y8m33ea	258	13	Loyter	Loyter	NNP
cord-021063-4y8m33ea	258	14	,	,	,
cord-021063-4y8m33ea	258	15	1990	1990	CD
cord-021063-4y8m33ea	258	16	)	)	-RRB-
cord-021063-4y8m33ea	258	17	.	.	.
cord-021063-4y8m33ea	259	1	Once	once	IN
cord-021063-4y8m33ea	259	2	the	the	DT
cord-021063-4y8m33ea	259	3	DNA	dna	NN
cord-021063-4y8m33ea	259	4	has	have	VBZ
cord-021063-4y8m33ea	259	5	successfully	successfully	RB
cord-021063-4y8m33ea	259	6	been	be	VBN
cord-021063-4y8m33ea	259	7	introduced	introduce	VBN
cord-021063-4y8m33ea	259	8	into	into	IN
cord-021063-4y8m33ea	259	9	the	the	DT
cord-021063-4y8m33ea	259	10	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	259	11	of	of	IN
cord-021063-4y8m33ea	259	12	the	the	DT
cord-021063-4y8m33ea	259	13	target	target	NN
cord-021063-4y8m33ea	259	14	cell	cell	NN
cord-021063-4y8m33ea	259	15	,	,	,
cord-021063-4y8m33ea	259	16	the	the	DT
cord-021063-4y8m33ea	259	17	factors	factor	NNS
cord-021063-4y8m33ea	259	18	governing	govern	VBG
cord-021063-4y8m33ea	259	19	expression	expression	NN
cord-021063-4y8m33ea	259	20	are	be	VBP
cord-021063-4y8m33ea	259	21	much	much	RB
cord-021063-4y8m33ea	259	22	less	less	RBR
cord-021063-4y8m33ea	259	23	well	well	RB
cord-021063-4y8m33ea	259	24	understood	understand	VBN
cord-021063-4y8m33ea	259	25	.	.	.
cord-021063-4y8m33ea	260	1	However	however	RB
cord-021063-4y8m33ea	260	2	,	,	,
cord-021063-4y8m33ea	260	3	proteins	protein	NNS
cord-021063-4y8m33ea	260	4	exist	exist	VBP
cord-021063-4y8m33ea	260	5	that	that	WDT
cord-021063-4y8m33ea	260	6	are	be	VBP
cord-021063-4y8m33ea	260	7	karyophilic	karyophilic	NNP
cord-021063-4y8m33ea	261	1	,	,	,
cord-021063-4y8m33ea	261	2	that	that	RB
cord-021063-4y8m33ea	261	3	is	is	RB
cord-021063-4y8m33ea	261	4	,	,	,
cord-021063-4y8m33ea	261	5	they	-PRON-	PRP
cord-021063-4y8m33ea	261	6	are	be	VBP
cord-021063-4y8m33ea	261	7	actively	actively	RB
cord-021063-4y8m33ea	261	8	transported	transport	VBN
cord-021063-4y8m33ea	261	9	into	into	IN
cord-021063-4y8m33ea	261	10	the	the	DT
cord-021063-4y8m33ea	261	11	nucleus	nucleus	NN
cord-021063-4y8m33ea	261	12	.	.	.
cord-021063-4y8m33ea	262	1	Two	two	CD
cord-021063-4y8m33ea	262	2	such	such	JJ
cord-021063-4y8m33ea	262	3	proteins	protein	NNS
cord-021063-4y8m33ea	262	4	are	be	VBP
cord-021063-4y8m33ea	262	5	the	the	DT
cord-021063-4y8m33ea	262	6	Semliki	Semliki	NNP
cord-021063-4y8m33ea	262	7	Forest	Forest	NNP
cord-021063-4y8m33ea	262	8	Virus	Virus	NNP
cord-021063-4y8m33ea	262	9	nucleocapsid	nucleocapsid	NN
cord-021063-4y8m33ea	262	10	protein	protein	NN
cord-021063-4y8m33ea	262	11	(	(	-LRB-
cord-021063-4y8m33ea	262	12	Michel	Michel	NNP
cord-021063-4y8m33ea	262	13	et	et	NNP
cord-021063-4y8m33ea	262	14	al	al	NNP
cord-021063-4y8m33ea	262	15	.	.	NNP
cord-021063-4y8m33ea	262	16	,	,	,
cord-021063-4y8m33ea	262	17	1990	1990	CD
cord-021063-4y8m33ea	262	18	)	)	-RRB-
cord-021063-4y8m33ea	262	19	and	and	CC
cord-021063-4y8m33ea	262	20	the	the	DT
cord-021063-4y8m33ea	262	21	non	non	JJ
cord-021063-4y8m33ea	262	22	-	-	JJ
cord-021063-4y8m33ea	262	23	histone	histone	JJ
cord-021063-4y8m33ea	262	24	high	high	JJ
cord-021063-4y8m33ea	262	25	mobility	mobility	NN
cord-021063-4y8m33ea	262	26	group	group	NN
cord-021063-4y8m33ea	262	27	I	-PRON-	PRP
cord-021063-4y8m33ea	262	28	chromosomal	chromosomal	JJ
cord-021063-4y8m33ea	262	29	protein	protein	NN
cord-021063-4y8m33ea	262	30	(	(	-LRB-
cord-021063-4y8m33ea	262	31	HMG	HMG	NNP
cord-021063-4y8m33ea	262	32	I	I	NNP
cord-021063-4y8m33ea	262	33	)	)	-RRB-
cord-021063-4y8m33ea	262	34	(	(	-LRB-
cord-021063-4y8m33ea	262	35	Kato	Kato	NNP
cord-021063-4y8m33ea	262	36	et	et	NNP
cord-021063-4y8m33ea	262	37	al	al	NNP
cord-021063-4y8m33ea	262	38	.	.	NNP
cord-021063-4y8m33ea	262	39	,	,	,
cord-021063-4y8m33ea	262	40	1991	1991	CD
cord-021063-4y8m33ea	262	41	;	;	:
cord-021063-4y8m33ea	262	42	Tomita	Tomita	NNP
cord-021063-4y8m33ea	262	43	et	et	NNP
cord-021063-4y8m33ea	262	44	al	al	NNP
cord-021063-4y8m33ea	262	45	.	.	NNP
cord-021063-4y8m33ea	262	46	,	,	,
cord-021063-4y8m33ea	262	47	1992	1992	CD
cord-021063-4y8m33ea	262	48	)	)	-RRB-
cord-021063-4y8m33ea	262	49	.	.	.
cord-021063-4y8m33ea	263	1	If	if	IN
cord-021063-4y8m33ea	263	2	a	a	DT
cord-021063-4y8m33ea	263	3	gene	gene	NN
cord-021063-4y8m33ea	263	4	is	be	VBZ
cord-021063-4y8m33ea	263	5	coencapsulated	coencapsulate	VBN
cord-021063-4y8m33ea	263	6	in	in	IN
cord-021063-4y8m33ea	263	7	a	a	DT
cord-021063-4y8m33ea	263	8	liposome	liposome	NN
cord-021063-4y8m33ea	263	9	with	with	IN
cord-021063-4y8m33ea	263	10	HMG	HMG	NNP
cord-021063-4y8m33ea	263	11	I	I	NNP
cord-021063-4y8m33ea	263	12	,	,	,
cord-021063-4y8m33ea	263	13	expression	expression	NN
cord-021063-4y8m33ea	263	14	increases	increase	VBZ
cord-021063-4y8m33ea	263	15	as	as	RB
cord-021063-4y8m33ea	263	16	much	much	RB
cord-021063-4y8m33ea	263	17	as	as	IN
cord-021063-4y8m33ea	263	18	10-fold	10-fold	JJ
cord-021063-4y8m33ea	263	19	over	over	IN
cord-021063-4y8m33ea	263	20	the	the	DT
cord-021063-4y8m33ea	263	21	levels	level	NNS
cord-021063-4y8m33ea	263	22	seen	see	VBN
cord-021063-4y8m33ea	263	23	without	without	IN
cord-021063-4y8m33ea	263	24	the	the	DT
cord-021063-4y8m33ea	263	25	protein	protein	NN
cord-021063-4y8m33ea	263	26	.	.	.
cord-021063-4y8m33ea	264	1	Delivery	delivery	NN
cord-021063-4y8m33ea	264	2	of	of	IN
cord-021063-4y8m33ea	264	3	DNA	dna	NN
cord-021063-4y8m33ea	264	4	/	/	SYM
cord-021063-4y8m33ea	264	5	protein	protein	NN
cord-021063-4y8m33ea	264	6	complexes	complex	NNS
cord-021063-4y8m33ea	264	7	has	have	VBZ
cord-021063-4y8m33ea	264	8	the	the	DT
cord-021063-4y8m33ea	264	9	potential	potential	NN
cord-021063-4y8m33ea	264	10	for	for	IN
cord-021063-4y8m33ea	264	11	greatly	greatly	RB
cord-021063-4y8m33ea	264	12	increasing	increase	VBG
cord-021063-4y8m33ea	264	13	transfection	transfection	NN
cord-021063-4y8m33ea	264	14	efficiency	efficiency	NN
cord-021063-4y8m33ea	264	15	,	,	,
cord-021063-4y8m33ea	264	16	and	and	CC
cord-021063-4y8m33ea	264	17	should	should	MD
cord-021063-4y8m33ea	264	18	prove	prove	VB
cord-021063-4y8m33ea	264	19	to	to	TO
cord-021063-4y8m33ea	264	20	be	be	VB
cord-021063-4y8m33ea	264	21	compatible	compatible	JJ
cord-021063-4y8m33ea	264	22	with	with	IN
cord-021063-4y8m33ea	264	23	most	most	JJS
cord-021063-4y8m33ea	264	24	methods	method	NNS
cord-021063-4y8m33ea	264	25	of	of	IN
cord-021063-4y8m33ea	264	26	liposomal	liposomal	JJ
cord-021063-4y8m33ea	264	27	preparation	preparation	NN
cord-021063-4y8m33ea	264	28	.	.	.
cord-021063-4y8m33ea	265	1	One	one	CD
cord-021063-4y8m33ea	265	2	shortcoming	shortcoming	NN
cord-021063-4y8m33ea	265	3	that	that	IN
cord-021063-4y8m33ea	265	4	all	all	DT
cord-021063-4y8m33ea	265	5	present	present	JJ
cord-021063-4y8m33ea	265	6	approaches	approach	NNS
cord-021063-4y8m33ea	265	7	to	to	TO
cord-021063-4y8m33ea	265	8	gene	gene	NN
cord-021063-4y8m33ea	265	9	therapy	therapy	NN
cord-021063-4y8m33ea	265	10	share	share	NN
cord-021063-4y8m33ea	265	11	is	be	VBZ
cord-021063-4y8m33ea	265	12	their	-PRON-	PRP$
cord-021063-4y8m33ea	265	13	inability	inability	NN
cord-021063-4y8m33ea	265	14	to	to	TO
cord-021063-4y8m33ea	265	15	maintain	maintain	VB
cord-021063-4y8m33ea	265	16	a	a	DT
cord-021063-4y8m33ea	265	17	high	high	JJ
cord-021063-4y8m33ea	265	18	level	level	NN
cord-021063-4y8m33ea	265	19	expression	expression	NN
cord-021063-4y8m33ea	265	20	of	of	IN
cord-021063-4y8m33ea	265	21	the	the	DT
cord-021063-4y8m33ea	265	22	transfected	transfecte	VBN
cord-021063-4y8m33ea	265	23	gene	gene	NN
cord-021063-4y8m33ea	265	24	for	for	IN
cord-021063-4y8m33ea	265	25	long	long	JJ
cord-021063-4y8m33ea	265	26	periods	period	NNS
cord-021063-4y8m33ea	265	27	of	of	IN
cord-021063-4y8m33ea	265	28	time	time	NN
cord-021063-4y8m33ea	265	29	.	.	.
cord-021063-4y8m33ea	266	1	For	for	IN
cord-021063-4y8m33ea	266	2	some	some	DT
cord-021063-4y8m33ea	266	3	applications	application	NNS
cord-021063-4y8m33ea	266	4	,	,	,
cord-021063-4y8m33ea	266	5	such	such	JJ
cord-021063-4y8m33ea	266	6	as	as	IN
cord-021063-4y8m33ea	266	7	the	the	DT
cord-021063-4y8m33ea	266	8	activation	activation	NN
cord-021063-4y8m33ea	266	9	of	of	IN
cord-021063-4y8m33ea	266	10	the	the	DT
cord-021063-4y8m33ea	266	11	immune	immune	JJ
cord-021063-4y8m33ea	266	12	system	system	NN
cord-021063-4y8m33ea	266	13	to	to	TO
cord-021063-4y8m33ea	266	14	fight	fight	VB
cord-021063-4y8m33ea	266	15	a	a	DT
cord-021063-4y8m33ea	266	16	cancer	cancer	NN
cord-021063-4y8m33ea	266	17	,	,	,
cord-021063-4y8m33ea	266	18	this	this	DT
cord-021063-4y8m33ea	266	19	will	will	MD
cord-021063-4y8m33ea	266	20	probably	probably	RB
cord-021063-4y8m33ea	266	21	not	not	RB
cord-021063-4y8m33ea	266	22	be	be	VB
cord-021063-4y8m33ea	266	23	a	a	DT
cord-021063-4y8m33ea	266	24	problem	problem	NN
cord-021063-4y8m33ea	266	25	.	.	.
cord-021063-4y8m33ea	267	1	However	however	RB
cord-021063-4y8m33ea	267	2	,	,	,
cord-021063-4y8m33ea	267	3	to	to	TO
cord-021063-4y8m33ea	267	4	effectively	effectively	RB
cord-021063-4y8m33ea	267	5	cure	cure	VB
cord-021063-4y8m33ea	267	6	many	many	JJ
cord-021063-4y8m33ea	267	7	genetic	genetic	JJ
cord-021063-4y8m33ea	267	8	diseases	disease	NNS
cord-021063-4y8m33ea	267	9	,	,	,
cord-021063-4y8m33ea	267	10	the	the	DT
cord-021063-4y8m33ea	267	11	gene	gene	NN
cord-021063-4y8m33ea	267	12	will	will	MD
cord-021063-4y8m33ea	267	13	need	need	VB
cord-021063-4y8m33ea	267	14	to	to	TO
cord-021063-4y8m33ea	267	15	be	be	VB
cord-021063-4y8m33ea	267	16	expressed	express	VBN
cord-021063-4y8m33ea	267	17	at	at	IN
cord-021063-4y8m33ea	267	18	near	near	IN
cord-021063-4y8m33ea	267	19	normal	normal	JJ
cord-021063-4y8m33ea	267	20	levels	level	NNS
cord-021063-4y8m33ea	267	21	throughout	throughout	IN
cord-021063-4y8m33ea	267	22	the	the	DT
cord-021063-4y8m33ea	267	23	patient	patient	NN
cord-021063-4y8m33ea	267	24	's	's	POS
cord-021063-4y8m33ea	267	25	life	life	NN
cord-021063-4y8m33ea	267	26	.	.	.
cord-021063-4y8m33ea	268	1	Successful	successful	JJ
cord-021063-4y8m33ea	268	2	transfection	transfection	NN
cord-021063-4y8m33ea	268	3	of	of	IN
cord-021063-4y8m33ea	268	4	cells	cell	NNS
cord-021063-4y8m33ea	268	5	with	with	IN
cord-021063-4y8m33ea	268	6	some	some	DT
cord-021063-4y8m33ea	268	7	currently	currently	RB
cord-021063-4y8m33ea	268	8	available	available	JJ
cord-021063-4y8m33ea	268	9	viral	viral	JJ
cord-021063-4y8m33ea	268	10	vectors	vector	NNS
cord-021063-4y8m33ea	268	11	may	may	MD
cord-021063-4y8m33ea	268	12	lead	lead	VB
cord-021063-4y8m33ea	268	13	to	to	IN
cord-021063-4y8m33ea	268	14	expression	expression	NN
cord-021063-4y8m33ea	268	15	of	of	IN
cord-021063-4y8m33ea	268	16	at	at	IN
cord-021063-4y8m33ea	268	17	least	least	JJS
cord-021063-4y8m33ea	268	18	some	some	DT
cord-021063-4y8m33ea	268	19	viral	viral	JJ
cord-021063-4y8m33ea	268	20	proteins	protein	NNS
cord-021063-4y8m33ea	268	21	,	,	,
cord-021063-4y8m33ea	268	22	resulting	result	VBG
cord-021063-4y8m33ea	268	23	in	in	IN
cord-021063-4y8m33ea	268	24	the	the	DT
cord-021063-4y8m33ea	268	25	clearance	clearance	NN
cord-021063-4y8m33ea	268	26	of	of	IN
cord-021063-4y8m33ea	268	27	the	the	DT
cord-021063-4y8m33ea	268	28	cells	cell	NNS
cord-021063-4y8m33ea	268	29	by	by	IN
cord-021063-4y8m33ea	268	30	the	the	DT
cord-021063-4y8m33ea	268	31	host	host	NN
cord-021063-4y8m33ea	268	32	's	's	POS
cord-021063-4y8m33ea	268	33	immune	immune	JJ
cord-021063-4y8m33ea	268	34	system	system	NN
cord-021063-4y8m33ea	268	35	.	.	.
cord-021063-4y8m33ea	269	1	This	this	DT
cord-021063-4y8m33ea	269	2	process	process	NN
cord-021063-4y8m33ea	269	3	may	may	MD
cord-021063-4y8m33ea	269	4	be	be	VB
cord-021063-4y8m33ea	269	5	partially	partially	RB
cord-021063-4y8m33ea	269	6	responsible	responsible	JJ
cord-021063-4y8m33ea	269	7	for	for	IN
cord-021063-4y8m33ea	269	8	the	the	DT
cord-021063-4y8m33ea	269	9	loss	loss	NN
cord-021063-4y8m33ea	269	10	of	of	IN
cord-021063-4y8m33ea	269	11	expression	expression	NN
cord-021063-4y8m33ea	269	12	seen	see	VBN
cord-021063-4y8m33ea	269	13	in	in	IN
cord-021063-4y8m33ea	269	14	transfections	transfection	NNS
cord-021063-4y8m33ea	269	15	with	with	IN
cord-021063-4y8m33ea	269	16	retroviral	retroviral	JJ
cord-021063-4y8m33ea	269	17	and	and	CC
cord-021063-4y8m33ea	269	18	adenoviral	adenoviral	JJ
cord-021063-4y8m33ea	269	19	vectors	vector	NNS
cord-021063-4y8m33ea	269	20	.	.	.
cord-021063-4y8m33ea	270	1	Another	another	DT
cord-021063-4y8m33ea	270	2	possible	possible	JJ
cord-021063-4y8m33ea	270	3	cause	cause	NN
cord-021063-4y8m33ea	270	4	of	of	IN
cord-021063-4y8m33ea	270	5	low	low	JJ
cord-021063-4y8m33ea	270	6	levels	level	NNS
cord-021063-4y8m33ea	270	7	of	of	IN
cord-021063-4y8m33ea	270	8	expression	expression	NN
cord-021063-4y8m33ea	270	9	is	be	VBZ
cord-021063-4y8m33ea	270	10	that	that	IN
cord-021063-4y8m33ea	270	11	length	length	NN
cord-021063-4y8m33ea	270	12	constraints	constraint	NNS
cord-021063-4y8m33ea	270	13	of	of	IN
cord-021063-4y8m33ea	270	14	viral	viral	JJ
cord-021063-4y8m33ea	270	15	vectors	vector	NNS
cord-021063-4y8m33ea	270	16	usually	usually	RB
cord-021063-4y8m33ea	270	17	necessitate	necessitate	VBP
cord-021063-4y8m33ea	270	18	the	the	DT
cord-021063-4y8m33ea	270	19	use	use	NN
cord-021063-4y8m33ea	270	20	of	of	IN
cord-021063-4y8m33ea	270	21	cDNA	cdna	NN
cord-021063-4y8m33ea	270	22	copies	copy	NNS
cord-021063-4y8m33ea	270	23	of	of	IN
cord-021063-4y8m33ea	270	24	genes	gene	NNS
cord-021063-4y8m33ea	270	25	.	.	.
cord-021063-4y8m33ea	271	1	These	these	DT
cord-021063-4y8m33ea	271	2	constructs	construct	NNS
cord-021063-4y8m33ea	271	3	do	do	VBP
cord-021063-4y8m33ea	271	4	not	not	RB
cord-021063-4y8m33ea	271	5	have	have	VB
cord-021063-4y8m33ea	271	6	introns	intron	NNS
cord-021063-4y8m33ea	271	7	,	,	,
cord-021063-4y8m33ea	271	8	greatly	greatly	RB
cord-021063-4y8m33ea	271	9	reducing	reduce	VBG
cord-021063-4y8m33ea	271	10	their	-PRON-	PRP$
cord-021063-4y8m33ea	271	11	length	length	NN
cord-021063-4y8m33ea	271	12	.	.	.
cord-021063-4y8m33ea	272	1	There	there	EX
cord-021063-4y8m33ea	272	2	is	be	VBZ
cord-021063-4y8m33ea	272	3	some	some	DT
cord-021063-4y8m33ea	272	4	evidence	evidence	NN
cord-021063-4y8m33ea	272	5	from	from	IN
cord-021063-4y8m33ea	272	6	transgenic	transgenic	JJ
cord-021063-4y8m33ea	272	7	mouse	mouse	NN
cord-021063-4y8m33ea	272	8	experiments	experiment	NNS
cord-021063-4y8m33ea	272	9	that	that	WDT
cord-021063-4y8m33ea	272	10	expression	expression	NN
cord-021063-4y8m33ea	272	11	of	of	IN
cord-021063-4y8m33ea	272	12	exogenous	exogenous	JJ
cord-021063-4y8m33ea	272	13	genes	gene	NNS
cord-021063-4y8m33ea	272	14	is	be	VBZ
cord-021063-4y8m33ea	272	15	higher	high	JJR
cord-021063-4y8m33ea	272	16	when	when	WRB
cord-021063-4y8m33ea	272	17	at	at	IN
cord-021063-4y8m33ea	272	18	least	least	JJS
cord-021063-4y8m33ea	272	19	some	some	DT
cord-021063-4y8m33ea	272	20	of	of	IN
cord-021063-4y8m33ea	272	21	the	the	DT
cord-021063-4y8m33ea	272	22	introns	intron	NNS
cord-021063-4y8m33ea	272	23	are	be	VBP
cord-021063-4y8m33ea	272	24	present	present	JJ
cord-021063-4y8m33ea	273	1	(	(	-LRB-
cord-021063-4y8m33ea	273	2	Brinster	Brinster	NNP
cord-021063-4y8m33ea	273	3	et	et	NNP
cord-021063-4y8m33ea	273	4	al	al	NNP
cord-021063-4y8m33ea	273	5	.	.	NNP
cord-021063-4y8m33ea	273	6	,	,	,
cord-021063-4y8m33ea	273	7	1988	1988	CD
cord-021063-4y8m33ea	273	8	;	;	:
cord-021063-4y8m33ea	273	9	Palmiter	Palmiter	NNP
cord-021063-4y8m33ea	273	10	et	et	NNP
cord-021063-4y8m33ea	273	11	al	al	NNP
cord-021063-4y8m33ea	273	12	.	.	NNP
cord-021063-4y8m33ea	273	13	,	,	,
cord-021063-4y8m33ea	273	14	1991	1991	CD
cord-021063-4y8m33ea	273	15	)	)	-RRB-
cord-021063-4y8m33ea	273	16	.	.	.
cord-021063-4y8m33ea	274	1	If	if	IN
cord-021063-4y8m33ea	274	2	this	this	DT
cord-021063-4y8m33ea	274	3	is	be	VBZ
cord-021063-4y8m33ea	274	4	true	true	JJ
cord-021063-4y8m33ea	274	5	for	for	IN
cord-021063-4y8m33ea	274	6	genes	gene	NNS
cord-021063-4y8m33ea	274	7	inserted	insert	VBN
cord-021063-4y8m33ea	274	8	by	by	IN
cord-021063-4y8m33ea	274	9	viral	viral	JJ
cord-021063-4y8m33ea	274	10	or	or	CC
cord-021063-4y8m33ea	274	11	liposomal	liposomal	JJ
cord-021063-4y8m33ea	274	12	methods	method	NNS
cord-021063-4y8m33ea	274	13	,	,	,
cord-021063-4y8m33ea	274	14	then	then	RB
cord-021063-4y8m33ea	274	15	it	-PRON-	PRP
cord-021063-4y8m33ea	274	16	may	may	MD
cord-021063-4y8m33ea	274	17	be	be	VB
cord-021063-4y8m33ea	274	18	necessary	necessary	JJ
cord-021063-4y8m33ea	274	19	to	to	TO
cord-021063-4y8m33ea	274	20	include	include	VB
cord-021063-4y8m33ea	274	21	introns	intron	NNS
cord-021063-4y8m33ea	274	22	in	in	IN
cord-021063-4y8m33ea	274	23	the	the	DT
cord-021063-4y8m33ea	274	24	delivered	deliver	VBN
cord-021063-4y8m33ea	274	25	DNA	DNA	NNP
cord-021063-4y8m33ea	274	26	to	to	TO
cord-021063-4y8m33ea	274	27	produce	produce	VB
cord-021063-4y8m33ea	274	28	an	an	DT
cord-021063-4y8m33ea	274	29	adequate	adequate	JJ
cord-021063-4y8m33ea	274	30	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	274	31	effect	effect	NN
cord-021063-4y8m33ea	274	32	.	.	.
cord-021063-4y8m33ea	275	1	Unfortunately	unfortunately	RB
cord-021063-4y8m33ea	275	2	,	,	,
cord-021063-4y8m33ea	275	3	increasing	increase	VBG
cord-021063-4y8m33ea	275	4	the	the	DT
cord-021063-4y8m33ea	275	5	size	size	NN
cord-021063-4y8m33ea	275	6	of	of	IN
cord-021063-4y8m33ea	275	7	the	the	DT
cord-021063-4y8m33ea	275	8	therapeutic	therapeutic	JJ
cord-021063-4y8m33ea	275	9	gene	gene	NN
cord-021063-4y8m33ea	275	10	would	would	MD
cord-021063-4y8m33ea	275	11	prevent	prevent	VB
cord-021063-4y8m33ea	275	12	the	the	DT
cord-021063-4y8m33ea	275	13	use	use	NN
cord-021063-4y8m33ea	275	14	of	of	IN
cord-021063-4y8m33ea	275	15	most	most	JJS
cord-021063-4y8m33ea	275	16	viral	viral	JJ
cord-021063-4y8m33ea	275	17	vectors	vector	NNS
cord-021063-4y8m33ea	275	18	.	.	.
cord-021063-4y8m33ea	276	1	While	while	IN
cord-021063-4y8m33ea	276	2	all	all	PDT
cord-021063-4y8m33ea	276	3	the	the	DT
cord-021063-4y8m33ea	276	4	methods	method	NNS
cord-021063-4y8m33ea	276	5	of	of	IN
cord-021063-4y8m33ea	276	6	gene	gene	NN
cord-021063-4y8m33ea	276	7	therapy	therapy	NN
cord-021063-4y8m33ea	276	8	have	have	VBP
cord-021063-4y8m33ea	276	9	specific	specific	JJ
cord-021063-4y8m33ea	276	10	advantages	advantage	NNS
cord-021063-4y8m33ea	276	11	,	,	,
cord-021063-4y8m33ea	276	12	none	none	NN
cord-021063-4y8m33ea	276	13	is	be	VBZ
cord-021063-4y8m33ea	276	14	suitable	suitable	JJ
cord-021063-4y8m33ea	276	15	for	for	IN
cord-021063-4y8m33ea	276	16	all	all	DT
cord-021063-4y8m33ea	276	17	applications	application	NNS
cord-021063-4y8m33ea	276	18	.	.	.
cord-021063-4y8m33ea	277	1	In	in	IN
cord-021063-4y8m33ea	277	2	an	an	DT
cord-021063-4y8m33ea	277	3	environment	environment	NN
cord-021063-4y8m33ea	277	4	as	as	RB
cord-021063-4y8m33ea	277	5	complex	complex	JJ
cord-021063-4y8m33ea	277	6	as	as	IN
cord-021063-4y8m33ea	277	7	a	a	DT
cord-021063-4y8m33ea	277	8	living	live	VBG
cord-021063-4y8m33ea	277	9	organism	organism	NN
cord-021063-4y8m33ea	277	10	,	,	,
cord-021063-4y8m33ea	277	11	it	-PRON-	PRP
cord-021063-4y8m33ea	277	12	is	be	VBZ
cord-021063-4y8m33ea	277	13	futile	futile	JJ
cord-021063-4y8m33ea	277	14	to	to	TO
cord-021063-4y8m33ea	277	15	hope	hope	VB
cord-021063-4y8m33ea	277	16	that	that	IN
cord-021063-4y8m33ea	277	17	a	a	DT
cord-021063-4y8m33ea	277	18	single	single	JJ
cord-021063-4y8m33ea	277	19	method	method	NN
cord-021063-4y8m33ea	277	20	might	may	MD
cord-021063-4y8m33ea	277	21	be	be	VB
cord-021063-4y8m33ea	277	22	ideal	ideal	JJ
cord-021063-4y8m33ea	277	23	in	in	IN
cord-021063-4y8m33ea	277	24	all	all	DT
cord-021063-4y8m33ea	277	25	situations	situation	NNS
cord-021063-4y8m33ea	277	26	.	.	.
cord-021063-4y8m33ea	278	1	However	however	RB
cord-021063-4y8m33ea	278	2	,	,	,
cord-021063-4y8m33ea	278	3	the	the	DT
cord-021063-4y8m33ea	278	4	availability	availability	NN
cord-021063-4y8m33ea	278	5	of	of	IN
cord-021063-4y8m33ea	278	6	many	many	JJ
cord-021063-4y8m33ea	278	7	methods	method	NNS
cord-021063-4y8m33ea	278	8	greatly	greatly	RB
cord-021063-4y8m33ea	278	9	increases	increase	VBZ
cord-021063-4y8m33ea	278	10	the	the	DT
cord-021063-4y8m33ea	278	11	number	number	NN
cord-021063-4y8m33ea	278	12	and	and	CC
cord-021063-4y8m33ea	278	13	types	type	NNS
cord-021063-4y8m33ea	278	14	of	of	IN
cord-021063-4y8m33ea	278	15	diseases	disease	NNS
cord-021063-4y8m33ea	278	16	that	that	WDT
cord-021063-4y8m33ea	278	17	can	can	MD
cord-021063-4y8m33ea	278	18	be	be	VB
cord-021063-4y8m33ea	278	19	treated	treat	VBN
cord-021063-4y8m33ea	278	20	.	.	.
cord-021063-4y8m33ea	279	1	Viruses	virus	NNS
cord-021063-4y8m33ea	279	2	have	have	VBP
cord-021063-4y8m33ea	279	3	evolved	evolve	VBN
cord-021063-4y8m33ea	279	4	such	such	JJ
cord-021063-4y8m33ea	279	5	that	that	IN
cord-021063-4y8m33ea	279	6	they	-PRON-	PRP
cord-021063-4y8m33ea	279	7	are	be	VBP
cord-021063-4y8m33ea	279	8	able	able	JJ
cord-021063-4y8m33ea	279	9	to	to	TO
cord-021063-4y8m33ea	279	10	efficiently	efficiently	RB
cord-021063-4y8m33ea	279	11	introduce	introduce	VB
cord-021063-4y8m33ea	279	12	and	and	CC
cord-021063-4y8m33ea	279	13	express	express	VB
cord-021063-4y8m33ea	279	14	exogenous	exogenous	JJ
cord-021063-4y8m33ea	279	15	genes	gene	NNS
cord-021063-4y8m33ea	279	16	(	(	-LRB-
cord-021063-4y8m33ea	279	17	i.e.	i.e.	FW
cord-021063-4y8m33ea	279	18	,	,	,
cord-021063-4y8m33ea	279	19	viral	viral	JJ
cord-021063-4y8m33ea	279	20	genes	gene	NNS
cord-021063-4y8m33ea	279	21	)	)	-RRB-
cord-021063-4y8m33ea	279	22	in	in	IN
cord-021063-4y8m33ea	279	23	eukaryotic	eukaryotic	JJ
cord-021063-4y8m33ea	279	24	cells	cell	NNS
cord-021063-4y8m33ea	279	25	.	.	.
cord-021063-4y8m33ea	280	1	However	however	RB
cord-021063-4y8m33ea	280	2	,	,	,
cord-021063-4y8m33ea	280	3	it	-PRON-	PRP
cord-021063-4y8m33ea	280	4	is	be	VBZ
cord-021063-4y8m33ea	280	5	important	important	JJ
cord-021063-4y8m33ea	280	6	to	to	TO
cord-021063-4y8m33ea	280	7	remember	remember	VB
cord-021063-4y8m33ea	280	8	that	that	IN
cord-021063-4y8m33ea	280	9	viruses	virus	NNS
cord-021063-4y8m33ea	280	10	have	have	VBP
cord-021063-4y8m33ea	280	11	been	be	VBN
cord-021063-4y8m33ea	280	12	optimized	optimize	VBN
cord-021063-4y8m33ea	280	13	by	by	IN
cord-021063-4y8m33ea	280	14	selective	selective	JJ
cord-021063-4y8m33ea	280	15	pressure	pressure	NN
cord-021063-4y8m33ea	280	16	to	to	TO
cord-021063-4y8m33ea	280	17	meet	meet	VB
cord-021063-4y8m33ea	280	18	criteria	criterion	NNS
cord-021063-4y8m33ea	280	19	that	that	WDT
cord-021063-4y8m33ea	280	20	are	be	VBP
cord-021063-4y8m33ea	280	21	not	not	RB
cord-021063-4y8m33ea	280	22	precisely	precisely	RB
cord-021063-4y8m33ea	280	23	those	those	DT
cord-021063-4y8m33ea	280	24	of	of	IN
cord-021063-4y8m33ea	280	25	gene	gene	NN
cord-021063-4y8m33ea	280	26	therapy	therapy	NN
cord-021063-4y8m33ea	280	27	.	.	.
cord-021063-4y8m33ea	281	1	In	in	IN
cord-021063-4y8m33ea	281	2	particular	particular	JJ
cord-021063-4y8m33ea	281	3	,	,	,
cord-021063-4y8m33ea	281	4	most	most	JJS
cord-021063-4y8m33ea	281	5	viruses	virus	NNS
cord-021063-4y8m33ea	281	6	need	need	VBP
cord-021063-4y8m33ea	281	7	to	to	TO
cord-021063-4y8m33ea	281	8	maintain	maintain	VB
cord-021063-4y8m33ea	281	9	high	high	JJ
cord-021063-4y8m33ea	281	10	-	-	HYPH
cord-021063-4y8m33ea	281	11	level	level	NN
cord-021063-4y8m33ea	281	12	expression	expression	NN
cord-021063-4y8m33ea	281	13	of	of	IN
cord-021063-4y8m33ea	281	14	their	-PRON-	PRP$
cord-021063-4y8m33ea	281	15	proteins	protein	NNS
cord-021063-4y8m33ea	281	16	for	for	IN
cord-021063-4y8m33ea	281	17	only	only	RB
cord-021063-4y8m33ea	281	18	a	a	DT
cord-021063-4y8m33ea	281	19	short	short	JJ
cord-021063-4y8m33ea	281	20	time	time	NN
cord-021063-4y8m33ea	281	21	,	,	,
cord-021063-4y8m33ea	281	22	and	and	CC
cord-021063-4y8m33ea	281	23	need	nee	MD
cord-021063-4y8m33ea	281	24	not	not	RB
cord-021063-4y8m33ea	281	25	be	be	VB
cord-021063-4y8m33ea	281	26	concerned	concern	VBN
cord-021063-4y8m33ea	281	27	with	with	IN
cord-021063-4y8m33ea	281	28	the	the	DT
cord-021063-4y8m33ea	281	29	viability	viability	NN
cord-021063-4y8m33ea	281	30	of	of	IN
cord-021063-4y8m33ea	281	31	the	the	DT
cord-021063-4y8m33ea	281	32	host	host	NN
cord-021063-4y8m33ea	281	33	cell	cell	NN
cord-021063-4y8m33ea	281	34	after	after	IN
cord-021063-4y8m33ea	281	35	infection	infection	NN
cord-021063-4y8m33ea	281	36	.	.	.
cord-021063-4y8m33ea	282	1	Attempts	attempt	NNS
cord-021063-4y8m33ea	282	2	to	to	TO
cord-021063-4y8m33ea	282	3	modify	modify	VB
cord-021063-4y8m33ea	282	4	a	a	DT
cord-021063-4y8m33ea	282	5	virus	virus	NN
cord-021063-4y8m33ea	282	6	into	into	IN
cord-021063-4y8m33ea	282	7	a	a	DT
cord-021063-4y8m33ea	282	8	gene	gene	NN
cord-021063-4y8m33ea	282	9	therapy	therapy	NN
cord-021063-4y8m33ea	282	10	vector	vector	NN
cord-021063-4y8m33ea	282	11	will	will	MD
cord-021063-4y8m33ea	282	12	be	be	VB
cord-021063-4y8m33ea	282	13	hampered	hamper	VBN
cord-021063-4y8m33ea	282	14	by	by	IN
cord-021063-4y8m33ea	282	15	this	this	DT
cord-021063-4y8m33ea	282	16	conflict	conflict	NN
cord-021063-4y8m33ea	282	17	.	.	.
cord-021063-4y8m33ea	283	1	Virus	virus	NN
cord-021063-4y8m33ea	283	2	-	-	HYPH
cord-021063-4y8m33ea	283	3	based	base	VBN
cord-021063-4y8m33ea	283	4	methods	method	NNS
cord-021063-4y8m33ea	283	5	of	of	IN
cord-021063-4y8m33ea	283	6	gene	gene	NN
cord-021063-4y8m33ea	283	7	therapy	therapy	NN
cord-021063-4y8m33ea	283	8	are	be	VBP
cord-021063-4y8m33ea	283	9	likely	likely	JJ
cord-021063-4y8m33ea	283	10	to	to	TO
cord-021063-4y8m33ea	283	11	be	be	VB
cord-021063-4y8m33ea	283	12	most	most	RBS
cord-021063-4y8m33ea	283	13	useful	useful	JJ
cord-021063-4y8m33ea	283	14	in	in	IN
cord-021063-4y8m33ea	283	15	applications	application	NNS
cord-021063-4y8m33ea	283	16	that	that	WDT
cord-021063-4y8m33ea	283	17	require	require	VBP
cord-021063-4y8m33ea	283	18	a	a	DT
cord-021063-4y8m33ea	283	19	burst	burst	NN
cord-021063-4y8m33ea	283	20	of	of	IN
cord-021063-4y8m33ea	283	21	high	high	JJ
cord-021063-4y8m33ea	283	22	-	-	HYPH
cord-021063-4y8m33ea	283	23	level	level	NN
cord-021063-4y8m33ea	283	24	expression	expression	NN
cord-021063-4y8m33ea	283	25	in	in	IN
cord-021063-4y8m33ea	283	26	many	many	JJ
cord-021063-4y8m33ea	283	27	of	of	IN
cord-021063-4y8m33ea	283	28	the	the	DT
cord-021063-4y8m33ea	283	29	patient	patient	NN
cord-021063-4y8m33ea	283	30	's	's	POS
cord-021063-4y8m33ea	283	31	cells	cell	NNS
cord-021063-4y8m33ea	283	32	,	,	,
cord-021063-4y8m33ea	283	33	such	such	JJ
cord-021063-4y8m33ea	283	34	as	as	IN
cord-021063-4y8m33ea	283	35	in	in	IN
cord-021063-4y8m33ea	283	36	cancer	cancer	NN
cord-021063-4y8m33ea	283	37	therapy	therapy	NN
cord-021063-4y8m33ea	283	38	.	.	.
cord-021063-4y8m33ea	284	1	Cationic	cationic	JJ
cord-021063-4y8m33ea	284	2	lipid	lipid	NN
cord-021063-4y8m33ea	284	3	-	-	HYPH
cord-021063-4y8m33ea	284	4	DNA	dna	NN
cord-021063-4y8m33ea	284	5	complexes	complex	NNS
cord-021063-4y8m33ea	284	6	will	will	MD
cord-021063-4y8m33ea	284	7	never	never	RB
cord-021063-4y8m33ea	284	8	be	be	VB
cord-021063-4y8m33ea	284	9	as	as	RB
cord-021063-4y8m33ea	284	10	selective	selective	JJ
cord-021063-4y8m33ea	284	11	as	as	IN
cord-021063-4y8m33ea	284	12	either	either	DT
cord-021063-4y8m33ea	284	13	virus	virus	NN
cord-021063-4y8m33ea	284	14	-	-	:
cord-021063-4y8m33ea	284	15	or	or	CC
cord-021063-4y8m33ea	284	16	conventional	conventional	JJ
cord-021063-4y8m33ea	284	17	liposome	liposome	NN
cord-021063-4y8m33ea	284	18	-	-	HYPH
cord-021063-4y8m33ea	284	19	based	base	VBN
cord-021063-4y8m33ea	284	20	gene	gene	NN
cord-021063-4y8m33ea	284	21	therapy	therapy	NN
cord-021063-4y8m33ea	284	22	.	.	.
cord-021063-4y8m33ea	285	1	However	however	RB
cord-021063-4y8m33ea	285	2	,	,	,
cord-021063-4y8m33ea	285	3	they	-PRON-	PRP
cord-021063-4y8m33ea	285	4	will	will	MD
cord-021063-4y8m33ea	285	5	remain	remain	VB
cord-021063-4y8m33ea	285	6	much	much	RB
cord-021063-4y8m33ea	285	7	easier	easy	JJR
cord-021063-4y8m33ea	285	8	to	to	TO
cord-021063-4y8m33ea	285	9	prepare	prepare	VB
cord-021063-4y8m33ea	285	10	and	and	CC
cord-021063-4y8m33ea	285	11	use	use	VB
cord-021063-4y8m33ea	285	12	.	.	.
cord-021063-4y8m33ea	286	1	The	the	DT
cord-021063-4y8m33ea	286	2	complexes	complex	NNS
cord-021063-4y8m33ea	286	3	will	will	MD
cord-021063-4y8m33ea	286	4	probably	probably	RB
cord-021063-4y8m33ea	286	5	find	find	VB
cord-021063-4y8m33ea	286	6	their	-PRON-	PRP$
cord-021063-4y8m33ea	286	7	greatest	great	JJS
cord-021063-4y8m33ea	286	8	use	use	NN
cord-021063-4y8m33ea	286	9	in	in	IN
cord-021063-4y8m33ea	286	10	in	in	IN
cord-021063-4y8m33ea	286	11	vitro	vitro	FW
cord-021063-4y8m33ea	286	12	and	and	CC
cord-021063-4y8m33ea	286	13	animal	animal	NN
cord-021063-4y8m33ea	286	14	gene	gene	NN
cord-021063-4y8m33ea	286	15	transfer	transfer	NN
cord-021063-4y8m33ea	286	16	.	.	.
cord-021063-4y8m33ea	287	1	Although	although	IN
cord-021063-4y8m33ea	287	2	they	-PRON-	PRP
cord-021063-4y8m33ea	287	3	are	be	VBP
cord-021063-4y8m33ea	287	4	currently	currently	RB
cord-021063-4y8m33ea	287	5	more	more	RBR
cord-021063-4y8m33ea	287	6	widely	widely	RB
cord-021063-4y8m33ea	287	7	used	use	VBN
cord-021063-4y8m33ea	287	8	in	in	IN
cord-021063-4y8m33ea	287	9	all	all	DT
cord-021063-4y8m33ea	287	10	areas	area	NNS
cord-021063-4y8m33ea	287	11	of	of	IN
cord-021063-4y8m33ea	287	12	gene	gene	NN
cord-021063-4y8m33ea	287	13	transfer	transfer	NN
cord-021063-4y8m33ea	287	14	than	than	IN
cord-021063-4y8m33ea	287	15	any	any	DT
cord-021063-4y8m33ea	287	16	other	other	JJ
cord-021063-4y8m33ea	287	17	technique	technique	NN
cord-021063-4y8m33ea	287	18	,	,	,
cord-021063-4y8m33ea	287	19	their	-PRON-	PRP$
cord-021063-4y8m33ea	287	20	lack	lack	NN
cord-021063-4y8m33ea	287	21	of	of	IN
cord-021063-4y8m33ea	287	22	control	control	NN
cord-021063-4y8m33ea	287	23	and	and	CC
cord-021063-4y8m33ea	287	24	cell	cell	NN
cord-021063-4y8m33ea	287	25	-	-	HYPH
cord-021063-4y8m33ea	287	26	specificity	specificity	NN
cord-021063-4y8m33ea	287	27	will	will	MD
cord-021063-4y8m33ea	287	28	probably	probably	RB
cord-021063-4y8m33ea	287	29	lead	lead	VB
cord-021063-4y8m33ea	287	30	to	to	IN
cord-021063-4y8m33ea	287	31	their	-PRON-	PRP$
cord-021063-4y8m33ea	287	32	replacement	replacement	NN
cord-021063-4y8m33ea	287	33	by	by	IN
cord-021063-4y8m33ea	287	34	viral	viral	JJ
cord-021063-4y8m33ea	287	35	and	and	CC
cord-021063-4y8m33ea	287	36	liposomal	liposomal	JJ
cord-021063-4y8m33ea	287	37	methods	method	NNS
cord-021063-4y8m33ea	287	38	of	of	IN
cord-021063-4y8m33ea	287	39	gene	gene	NN
cord-021063-4y8m33ea	287	40	introduction	introduction	NN
cord-021063-4y8m33ea	287	41	in	in	IN
cord-021063-4y8m33ea	287	42	clinical	clinical	JJ
cord-021063-4y8m33ea	287	43	areas	area	NNS
cord-021063-4y8m33ea	287	44	.	.	.
cord-021063-4y8m33ea	288	1	Liposomal	Liposomal	NNP
cord-021063-4y8m33ea	288	2	methods	method	NNS
cord-021063-4y8m33ea	288	3	of	of	IN
cord-021063-4y8m33ea	288	4	gene	gene	NN
cord-021063-4y8m33ea	288	5	therapy	therapy	NN
cord-021063-4y8m33ea	288	6	are	be	VBP
cord-021063-4y8m33ea	288	7	flexible	flexible	JJ
cord-021063-4y8m33ea	288	8	,	,	,
cord-021063-4y8m33ea	288	9	in	in	IN
cord-021063-4y8m33ea	288	10	that	that	DT
cord-021063-4y8m33ea	288	11	all	all	PDT
cord-021063-4y8m33ea	288	12	the	the	DT
cord-021063-4y8m33ea	288	13	components	component	NNS
cord-021063-4y8m33ea	288	14	of	of	IN
cord-021063-4y8m33ea	288	15	the	the	DT
cord-021063-4y8m33ea	288	16	system	system	NN
cord-021063-4y8m33ea	288	17	are	be	VBP
cord-021063-4y8m33ea	288	18	controlled	control	VBN
cord-021063-4y8m33ea	288	19	by	by	IN
cord-021063-4y8m33ea	288	20	the	the	DT
cord-021063-4y8m33ea	288	21	designers	designer	NNS
cord-021063-4y8m33ea	288	22	.	.	.
cord-021063-4y8m33ea	289	1	As	as	IN
cord-021063-4y8m33ea	289	2	these	these	DT
cord-021063-4y8m33ea	289	3	systems	system	NNS
cord-021063-4y8m33ea	289	4	have	have	VBP
cord-021063-4y8m33ea	289	5	become	become	VBN
cord-021063-4y8m33ea	289	6	more	more	RBR
cord-021063-4y8m33ea	289	7	sophisticated	sophisticated	JJ
cord-021063-4y8m33ea	289	8	,	,	,
cord-021063-4y8m33ea	289	9	they	-PRON-	PRP
cord-021063-4y8m33ea	289	10	have	have	VBP
cord-021063-4y8m33ea	289	11	begun	begin	VBN
cord-021063-4y8m33ea	289	12	to	to	TO
cord-021063-4y8m33ea	289	13	take	take	VB
cord-021063-4y8m33ea	289	14	on	on	RP
cord-021063-4y8m33ea	289	15	several	several	JJ
cord-021063-4y8m33ea	289	16	characteristics	characteristic	NNS
cord-021063-4y8m33ea	289	17	of	of	IN
cord-021063-4y8m33ea	289	18	the	the	DT
cord-021063-4y8m33ea	289	19	viruses	virus	NNS
cord-021063-4y8m33ea	289	20	that	that	WDT
cord-021063-4y8m33ea	289	21	they	-PRON-	PRP
cord-021063-4y8m33ea	289	22	are	be	VBP
cord-021063-4y8m33ea	289	23	intended	intend	VBN
cord-021063-4y8m33ea	289	24	to	to	TO
cord-021063-4y8m33ea	289	25	replace	replace	VB
cord-021063-4y8m33ea	289	26	.	.	.
cord-021063-4y8m33ea	290	1	The	the	DT
cord-021063-4y8m33ea	290	2	use	use	NN
cord-021063-4y8m33ea	290	3	of	of	IN
cord-021063-4y8m33ea	290	4	basic	basic	JJ
cord-021063-4y8m33ea	290	5	substances	substance	NNS
cord-021063-4y8m33ea	290	6	to	to	TO
cord-021063-4y8m33ea	290	7	condense	condense	VB
cord-021063-4y8m33ea	290	8	the	the	DT
cord-021063-4y8m33ea	290	9	DNA	dna	NN
cord-021063-4y8m33ea	290	10	has	have	VBZ
cord-021063-4y8m33ea	290	11	increased	increase	VBN
cord-021063-4y8m33ea	290	12	the	the	DT
cord-021063-4y8m33ea	290	13	efficiency	efficiency	NN
cord-021063-4y8m33ea	290	14	of	of	IN
cord-021063-4y8m33ea	290	15	encapsulation	encapsulation	NN
cord-021063-4y8m33ea	290	16	.	.	.
cord-021063-4y8m33ea	291	1	The	the	DT
cord-021063-4y8m33ea	291	2	addition	addition	NN
cord-021063-4y8m33ea	291	3	of	of	IN
cord-021063-4y8m33ea	291	4	nucleophilic	nucleophilic	JJ
cord-021063-4y8m33ea	291	5	proteins	protein	NNS
cord-021063-4y8m33ea	291	6	raises	raise	VBZ
cord-021063-4y8m33ea	291	7	the	the	DT
cord-021063-4y8m33ea	291	8	efficiency	efficiency	NN
cord-021063-4y8m33ea	291	9	of	of	IN
cord-021063-4y8m33ea	291	10	transfection	transfection	NN
cord-021063-4y8m33ea	291	11	.	.	.
cord-021063-4y8m33ea	292	1	By	by	IN
cord-021063-4y8m33ea	292	2	adding	add	VBG
cord-021063-4y8m33ea	292	3	antibodies	antibody	NNS
cord-021063-4y8m33ea	292	4	or	or	CC
cord-021063-4y8m33ea	292	5	other	other	JJ
cord-021063-4y8m33ea	292	6	targeting	target	VBG
cord-021063-4y8m33ea	292	7	molecules	molecule	NNS
cord-021063-4y8m33ea	292	8	to	to	IN
cord-021063-4y8m33ea	292	9	the	the	DT
cord-021063-4y8m33ea	292	10	surface	surface	NN
cord-021063-4y8m33ea	292	11	of	of	IN
cord-021063-4y8m33ea	292	12	liposomes	liposome	NNS
cord-021063-4y8m33ea	292	13	,	,	,
cord-021063-4y8m33ea	292	14	preferential	preferential	JJ
cord-021063-4y8m33ea	292	15	binding	binding	NN
cord-021063-4y8m33ea	292	16	of	of	IN
cord-021063-4y8m33ea	292	17	vesicles	vesicle	NNS
cord-021063-4y8m33ea	292	18	to	to	IN
cord-021063-4y8m33ea	292	19	a	a	DT
cord-021063-4y8m33ea	292	20	desired	desire	VBN
cord-021063-4y8m33ea	292	21	cell	cell	NN
cord-021063-4y8m33ea	292	22	type	type	NN
cord-021063-4y8m33ea	292	23	has	have	VBZ
cord-021063-4y8m33ea	292	24	been	be	VBN
cord-021063-4y8m33ea	292	25	increased	increase	VBN
cord-021063-4y8m33ea	292	26	.	.	.
cord-021063-4y8m33ea	293	1	The	the	DT
cord-021063-4y8m33ea	293	2	incorporation	incorporation	NN
cord-021063-4y8m33ea	293	3	of	of	IN
cord-021063-4y8m33ea	293	4	viral	viral	JJ
cord-021063-4y8m33ea	293	5	fusion	fusion	NN
cord-021063-4y8m33ea	293	6	proteins	protein	NNS
cord-021063-4y8m33ea	293	7	into	into	IN
cord-021063-4y8m33ea	293	8	the	the	DT
cord-021063-4y8m33ea	293	9	membrane	membrane	NN
cord-021063-4y8m33ea	293	10	allows	allow	VBZ
cord-021063-4y8m33ea	293	11	a	a	DT
cord-021063-4y8m33ea	293	12	greater	great	JJR
cord-021063-4y8m33ea	293	13	fraction	fraction	NN
cord-021063-4y8m33ea	293	14	of	of	IN
cord-021063-4y8m33ea	293	15	liposomes	liposome	NNS
cord-021063-4y8m33ea	293	16	binding	bind	VBG
cord-021063-4y8m33ea	293	17	to	to	IN
cord-021063-4y8m33ea	293	18	cells	cell	NNS
cord-021063-4y8m33ea	293	19	to	to	TO
cord-021063-4y8m33ea	293	20	deliver	deliver	VB
cord-021063-4y8m33ea	293	21	their	-PRON-	PRP$
cord-021063-4y8m33ea	293	22	contents	content	NNS
cord-021063-4y8m33ea	293	23	into	into	IN
cord-021063-4y8m33ea	293	24	the	the	DT
cord-021063-4y8m33ea	293	25	cytoplasm	cytoplasm	NN
cord-021063-4y8m33ea	293	26	.	.	.
cord-021063-4y8m33ea	294	1	In	in	IN
cord-021063-4y8m33ea	294	2	short	short	JJ
cord-021063-4y8m33ea	294	3	,	,	,
cord-021063-4y8m33ea	294	4	many	many	JJ
cord-021063-4y8m33ea	294	5	aspects	aspect	NNS
cord-021063-4y8m33ea	294	6	of	of	IN
cord-021063-4y8m33ea	294	7	a	a	DT
cord-021063-4y8m33ea	294	8	virus	virus	NN
cord-021063-4y8m33ea	294	9	from	from	IN
cord-021063-4y8m33ea	294	10	budding	bud	VBG
cord-021063-4y8m33ea	294	11	to	to	IN
cord-021063-4y8m33ea	294	12	delivery	delivery	NN
cord-021063-4y8m33ea	294	13	of	of	IN
cord-021063-4y8m33ea	294	14	the	the	DT
cord-021063-4y8m33ea	294	15	genetic	genetic	JJ
cord-021063-4y8m33ea	294	16	material	material	NN
cord-021063-4y8m33ea	294	17	to	to	IN
cord-021063-4y8m33ea	294	18	the	the	DT
cord-021063-4y8m33ea	294	19	nucleus	nucleus	NN
cord-021063-4y8m33ea	294	20	have	have	VBP
cord-021063-4y8m33ea	294	21	been	be	VBN
cord-021063-4y8m33ea	294	22	mimicked	mimic	VBN
cord-021063-4y8m33ea	294	23	in	in	IN
cord-021063-4y8m33ea	294	24	liposomal	liposomal	JJ
cord-021063-4y8m33ea	294	25	vectors	vector	NNS
cord-021063-4y8m33ea	294	26	.	.	.
cord-021063-4y8m33ea	295	1	Current	current	JJ
cord-021063-4y8m33ea	295	2	methods	method	NNS
cord-021063-4y8m33ea	295	3	of	of	IN
cord-021063-4y8m33ea	295	4	liposome	liposome	NN
cord-021063-4y8m33ea	295	5	-	-	HYPH
cord-021063-4y8m33ea	295	6	based	base	VBN
cord-021063-4y8m33ea	295	7	gene	gene	NN
cord-021063-4y8m33ea	295	8	therapy	therapy	NN
cord-021063-4y8m33ea	295	9	may	may	MD
cord-021063-4y8m33ea	295	10	be	be	VB
cord-021063-4y8m33ea	295	11	improved	improve	VBN
cord-021063-4y8m33ea	295	12	by	by	IN
cord-021063-4y8m33ea	295	13	modifying	modify	VBG
cord-021063-4y8m33ea	295	14	the	the	DT
cord-021063-4y8m33ea	295	15	structure	structure	NN
cord-021063-4y8m33ea	295	16	of	of	IN
cord-021063-4y8m33ea	295	17	the	the	DT
cord-021063-4y8m33ea	295	18	introduced	introduce	VBN
cord-021063-4y8m33ea	295	19	gene	gene	NN
cord-021063-4y8m33ea	295	20	.	.	.
cord-021063-4y8m33ea	296	1	Here	here	RB
cord-021063-4y8m33ea	296	2	,	,	,
cord-021063-4y8m33ea	296	3	preliminary	preliminary	JJ
cord-021063-4y8m33ea	296	4	indications	indication	NNS
cord-021063-4y8m33ea	296	5	are	be	VBP
cord-021063-4y8m33ea	296	6	that	that	IN
cord-021063-4y8m33ea	296	7	constructs	construct	NNS
cord-021063-4y8m33ea	296	8	resembling	resemble	VBG
cord-021063-4y8m33ea	296	9	native	native	JJ
cord-021063-4y8m33ea	296	10	genes	gene	NNS
cord-021063-4y8m33ea	296	11	,	,	,
cord-021063-4y8m33ea	296	12	rather	rather	RB
cord-021063-4y8m33ea	296	13	than	than	IN
cord-021063-4y8m33ea	296	14	cDNAs	cdna	NNS
cord-021063-4y8m33ea	296	15	,	,	,
cord-021063-4y8m33ea	296	16	will	will	MD
cord-021063-4y8m33ea	296	17	provide	provide	VB
cord-021063-4y8m33ea	296	18	the	the	DT
cord-021063-4y8m33ea	296	19	best	good	JJS
cord-021063-4y8m33ea	296	20	long	long	JJ
cord-021063-4y8m33ea	296	21	-	-	HYPH
cord-021063-4y8m33ea	296	22	term	term	NN
cord-021063-4y8m33ea	296	23	expression	expression	NN
cord-021063-4y8m33ea	296	24	.	.	.
cord-021063-4y8m33ea	297	1	Not	not	RB
cord-021063-4y8m33ea	297	2	all	all	PDT
cord-021063-4y8m33ea	297	3	these	these	DT
cord-021063-4y8m33ea	297	4	enhancements	enhancement	NNS
cord-021063-4y8m33ea	297	5	to	to	IN
cord-021063-4y8m33ea	297	6	the	the	DT
cord-021063-4y8m33ea	297	7	basic	basic	JJ
cord-021063-4y8m33ea	297	8	liposome	liposome	NN
cord-021063-4y8m33ea	297	9	have	have	VBP
cord-021063-4y8m33ea	297	10	been	be	VBN
cord-021063-4y8m33ea	297	11	used	use	VBN
cord-021063-4y8m33ea	297	12	together	together	RB
cord-021063-4y8m33ea	297	13	,	,	,
cord-021063-4y8m33ea	297	14	and	and	CC
cord-021063-4y8m33ea	297	15	not	not	RB
cord-021063-4y8m33ea	297	16	all	all	DT
cord-021063-4y8m33ea	297	17	of	of	IN
cord-021063-4y8m33ea	297	18	them	-PRON-	PRP
cord-021063-4y8m33ea	297	19	are	be	VBP
cord-021063-4y8m33ea	297	20	mutually	mutually	RB
cord-021063-4y8m33ea	297	21	compatible	compatible	JJ
cord-021063-4y8m33ea	297	22	.	.	.
cord-021063-4y8m33ea	298	1	However	however	RB
cord-021063-4y8m33ea	298	2	,	,	,
cord-021063-4y8m33ea	298	3	it	-PRON-	PRP
cord-021063-4y8m33ea	298	4	seems	seem	VBZ
cord-021063-4y8m33ea	298	5	inevitable	inevitable	JJ
cord-021063-4y8m33ea	298	6	that	that	IN
cord-021063-4y8m33ea	298	7	the	the	DT
cord-021063-4y8m33ea	298	8	best	good	JJS
cord-021063-4y8m33ea	298	9	liposome	liposome	NN
cord-021063-4y8m33ea	298	10	-	-	HYPH
cord-021063-4y8m33ea	298	11	based	base	VBN
cord-021063-4y8m33ea	298	12	gene	gene	NN
cord-021063-4y8m33ea	298	13	therapy	therapy	NN
cord-021063-4y8m33ea	298	14	vectors	vector	NNS
cord-021063-4y8m33ea	298	15	will	will	MD
cord-021063-4y8m33ea	298	16	eventually	eventually	RB
cord-021063-4y8m33ea	298	17	combine	combine	VB
cord-021063-4y8m33ea	298	18	many	many	JJ
cord-021063-4y8m33ea	298	19	of	of	IN
cord-021063-4y8m33ea	298	20	these	these	DT
cord-021063-4y8m33ea	298	21	enhancements	enhancement	NNS
cord-021063-4y8m33ea	298	22	.	.	.
cord-021063-4y8m33ea	299	1	The	the	DT
cord-021063-4y8m33ea	299	2	resulting	result	VBG
cord-021063-4y8m33ea	299	3	liposome	liposome	NN
cord-021063-4y8m33ea	299	4	,	,	,
cord-021063-4y8m33ea	299	5	with	with	IN
cord-021063-4y8m33ea	299	6	a	a	DT
cord-021063-4y8m33ea	299	7	condensed	condense	VBN
cord-021063-4y8m33ea	299	8	DNA	dna	NN
cord-021063-4y8m33ea	299	9	-	-	HYPH
cord-021063-4y8m33ea	299	10	protein	protein	NN
cord-021063-4y8m33ea	299	11	complex	complex	NN
cord-021063-4y8m33ea	299	12	containing	contain	VBG
cord-021063-4y8m33ea	299	13	both	both	DT
cord-021063-4y8m33ea	299	14	histonelike	histonelike	JJ
cord-021063-4y8m33ea	299	15	proteins	protein	NNS
cord-021063-4y8m33ea	299	16	and	and	CC
cord-021063-4y8m33ea	299	17	nuclear	nuclear	JJ
cord-021063-4y8m33ea	299	18	targeting	targeting	NN
cord-021063-4y8m33ea	299	19	proteins	protein	NNS
cord-021063-4y8m33ea	299	20	,	,	,
cord-021063-4y8m33ea	299	21	a	a	DT
cord-021063-4y8m33ea	299	22	membrane	membrane	NN
cord-021063-4y8m33ea	299	23	designed	design	VBN
cord-021063-4y8m33ea	299	24	for	for	IN
cord-021063-4y8m33ea	299	25	long	long	JJ
cord-021063-4y8m33ea	299	26	circulation	circulation	NN
cord-021063-4y8m33ea	299	27	within	within	IN
cord-021063-4y8m33ea	299	28	the	the	DT
cord-021063-4y8m33ea	299	29	blood	blood	NN
cord-021063-4y8m33ea	299	30	,	,	,
cord-021063-4y8m33ea	299	31	targeting	target	VBG
cord-021063-4y8m33ea	299	32	molecules	molecule	NNS
cord-021063-4y8m33ea	299	33	on	on	IN
cord-021063-4y8m33ea	299	34	the	the	DT
cord-021063-4y8m33ea	299	35	surface	surface	NN
cord-021063-4y8m33ea	299	36	,	,	,
cord-021063-4y8m33ea	299	37	and	and	CC
cord-021063-4y8m33ea	299	38	viral	viral	JJ
cord-021063-4y8m33ea	299	39	fusion	fusion	NN
cord-021063-4y8m33ea	299	40	proteins	protein	NNS
cord-021063-4y8m33ea	299	41	in	in	IN
cord-021063-4y8m33ea	299	42	the	the	DT
cord-021063-4y8m33ea	299	43	membrane	membrane	NN
cord-021063-4y8m33ea	299	44	,	,	,
cord-021063-4y8m33ea	299	45	will	will	MD
cord-021063-4y8m33ea	299	46	in	in	IN
cord-021063-4y8m33ea	299	47	effect	effect	NN
cord-021063-4y8m33ea	299	48	be	be	VB
cord-021063-4y8m33ea	299	49	a	a	DT
cord-021063-4y8m33ea	299	50	completely	completely	RB
cord-021063-4y8m33ea	299	51	man	man	NN
cord-021063-4y8m33ea	299	52	-	-	HYPH
cord-021063-4y8m33ea	299	53	made	make	VBN
cord-021063-4y8m33ea	299	54	nonreplicative	nonreplicative	NNP
cord-021063-4y8m33ea	299	55	virus	virus	NN
cord-021063-4y8m33ea	299	56	.	.	.
cord-021063-4y8m33ea	299	57	 	 	_SP
cord-021063-4y8m33ea	300	1	Liposomes	liposome	VBN
cord-021063-4y8m33ea	300	2	with	with	IN
cord-021063-4y8m33ea	300	3	prolonged	prolonged	JJ
cord-021063-4y8m33ea	300	4	circulation	circulation	NN
cord-021063-4y8m33ea	300	5	times	time	NNS
cord-021063-4y8m33ea	300	6	:	:	:
cord-021063-4y8m33ea	300	7	factors	factor	NNS
cord-021063-4y8m33ea	300	8	affecting	affect	VBG
cord-021063-4y8m33ea	300	9	uptake	uptake	NN
cord-021063-4y8m33ea	300	10	by	by	IN
cord-021063-4y8m33ea	300	11	reticuloendothelial	reticuloendothelial	JJ
cord-021063-4y8m33ea	300	12	and	and	CC
cord-021063-4y8m33ea	300	13	other	other	JJ
cord-021063-4y8m33ea	300	14	tissues	tissue	NNS
cord-021063-4y8m33ea	301	1	Molecular	molecular	JJ
cord-021063-4y8m33ea	301	2	mechanism	mechanism	NN
cord-021063-4y8m33ea	301	3	of	of	IN
cord-021063-4y8m33ea	301	4	the	the	DT
cord-021063-4y8m33ea	301	5	lipid	lipid	NN
cord-021063-4y8m33ea	301	6	vesicle	vesicle	NN
cord-021063-4y8m33ea	301	7	longevity	longevity	NN
cord-021063-4y8m33ea	301	8	in	in	IN
cord-021063-4y8m33ea	301	9	vivo	vivo	NNP
cord-021063-4y8m33ea	301	10	Expression	expression	NN
cord-021063-4y8m33ea	301	11	of	of	IN
cord-021063-4y8m33ea	301	12	human	human	JJ
cord-021063-4y8m33ea	301	13	growth	growth	NN
cord-021063-4y8m33ea	301	14	hormone	hormone	NN
cord-021063-4y8m33ea	301	15	fusion	fusion	NN
cord-021063-4y8m33ea	301	16	genes	gene	NNS
cord-021063-4y8m33ea	301	17	in	in	IN
cord-021063-4y8m33ea	301	18	cultured	cultured	JJ
cord-021063-4y8m33ea	301	19	lung	lung	NN
cord-021063-4y8m33ea	301	20	endothelial	endothelial	NN
cord-021063-4y8m33ea	301	21	cells	cell	NNS
cord-021063-4y8m33ea	301	22	and	and	CC
cord-021063-4y8m33ea	301	23	in	in	IN
cord-021063-4y8m33ea	301	24	the	the	DT
cord-021063-4y8m33ea	301	25	lungs	lung	NNS
cord-021063-4y8m33ea	301	26	of	of	IN
cord-021063-4y8m33ea	301	27	mice	mice	NN
cord-021063-4y8m33ea	301	28	lntrons	lntron	NNS
cord-021063-4y8m33ea	301	29	increase	increase	VBP
cord-021063-4y8m33ea	301	30	transcriptional	transcriptional	JJ
cord-021063-4y8m33ea	301	31	efficiency	efficiency	NN
cord-021063-4y8m33ea	301	32	in	in	IN
cord-021063-4y8m33ea	301	33	transgenic	transgenic	JJ
cord-021063-4y8m33ea	301	34	mice	mouse	NNS
cord-021063-4y8m33ea	301	35	Modulation	modulation	NN
cord-021063-4y8m33ea	301	36	of	of	IN
cord-021063-4y8m33ea	301	37	coronavirus	coronavirus	NN
cord-021063-4y8m33ea	301	38	-	-	HYPH
cord-021063-4y8m33ea	301	39	mediated	mediate	VBN
cord-021063-4y8m33ea	301	40	cell	cell	NN
cord-021063-4y8m33ea	301	41	fusion	fusion	NN
cord-021063-4y8m33ea	301	42	by	by	IN
cord-021063-4y8m33ea	301	43	homeostatic	homeostatic	JJ
cord-021063-4y8m33ea	301	44	control	control	NN
cord-021063-4y8m33ea	301	45	of	of	IN
cord-021063-4y8m33ea	301	46	cholesterol	cholesterol	NN
cord-021063-4y8m33ea	301	47	and	and	CC
cord-021063-4y8m33ea	301	48	fatty	fatty	JJ
cord-021063-4y8m33ea	301	49	acid	acid	NN
cord-021063-4y8m33ea	301	50	metabolism	metabolism	NN
cord-021063-4y8m33ea	301	51	Gene	Gene	NNP
cord-021063-4y8m33ea	301	52	therapy	therapy	NN
cord-021063-4y8m33ea	301	53	strategies	strategy	NNS
cord-021063-4y8m33ea	301	54	for	for	IN
cord-021063-4y8m33ea	301	55	pulmonary	pulmonary	JJ
cord-021063-4y8m33ea	301	56	disease	disease	NN
cord-021063-4y8m33ea	301	57	19q_'2	19q_'2	NNP
cord-021063-4y8m33ea	301	58	)	)	-RRB-
cord-021063-4y8m33ea	301	59	Effect	effect	NN
cord-021063-4y8m33ea	301	60	of	of	IN
cord-021063-4y8m33ea	301	61	cationic	cationic	JJ
cord-021063-4y8m33ea	301	62	cho!e	cho!e	JJ
cord-021063-4y8m33ea	302	1	~terol	~terol	NFP
cord-021063-4y8m33ea	302	2	derivatives	derivative	NNS
cord-021063-4y8m33ea	302	3	on	on	IN
cord-021063-4y8m33ea	302	4	gene	gene	NN
cord-021063-4y8m33ea	302	5	transfer	transfer	NN
cord-021063-4y8m33ea	302	6	and	and	CC
cord-021063-4y8m33ea	302	7	protein	protein	NN
cord-021063-4y8m33ea	302	8	kinase	kinase	NN
cord-021063-4y8m33ea	302	9	C	c	NN
cord-021063-4y8m33ea	302	10	activity	activity	NN
cord-021063-4y8m33ea	302	11	Introduction	introduction	NN
cord-021063-4y8m33ea	302	12	of	of	IN
cord-021063-4y8m33ea	302	13	liposome	liposome	NN
cord-021063-4y8m33ea	302	14	-	-	HYPH
cord-021063-4y8m33ea	302	15	encapsulated	encapsulate	VBN
cord-021063-4y8m33ea	302	16	SV40	SV40	NNP
cord-021063-4y8m33ea	302	17	DNA	DNA	NNP
cord-021063-4y8m33ea	302	18	into	into	IN
cord-021063-4y8m33ea	302	19	cells	cell	NNS
cord-021063-4y8m33ea	303	1	The	the	DT
cord-021063-4y8m33ea	303	2	role	role	NN
cord-021063-4y8m33ea	303	3	of	of	IN
cord-021063-4y8m33ea	303	4	surface	surface	NN
cord-021063-4y8m33ea	303	5	charge	charge	NN
cord-021063-4y8m33ea	303	6	and	and	CC
cord-021063-4y8m33ea	303	7	hydrophilic	hydrophilic	JJ
cord-021063-4y8m33ea	303	8	groups	group	NNS
cord-021063-4y8m33ea	303	9	on	on	IN
cord-021063-4y8m33ea	303	10	liposome	liposome	NN
cord-021063-4y8m33ea	303	11	clearance	clearance	NN
cord-021063-4y8m33ea	303	12	in	in	IN
cord-021063-4y8m33ea	303	13	vivo	vivo	NNP
cord-021063-4y8m33ea	303	14	A	a	DT
cord-021063-4y8m33ea	303	15	novel	novel	JJ
cord-021063-4y8m33ea	303	16	cationic	cationic	JJ
cord-021063-4y8m33ea	303	17	liposome	liposome	NN
cord-021063-4y8m33ea	303	18	reagent	reagent	NN
cord-021063-4y8m33ea	303	19	for	for	IN
cord-021063-4y8m33ea	303	20	efficient	efficient	JJ
cord-021063-4y8m33ea	303	21	transfection	transfection	NN
cord-021063-4y8m33ea	303	22	of	of	IN
cord-021063-4y8m33ea	303	23	mammalian	mammalian	JJ
cord-021063-4y8m33ea	303	24	cells	cell	NNS
cord-021063-4y8m33ea	303	25	Herpesviruses	Herpesviruses	NNP
cord-021063-4y8m33ea	303	26	:	:	:
cord-021063-4y8m33ea	303	27	expression	expression	NN
cord-021063-4y8m33ea	303	28	of	of	IN
cord-021063-4y8m33ea	303	29	genes	gene	NNS
cord-021063-4y8m33ea	303	30	in	in	IN
cord-021063-4y8m33ea	303	31	postmitotic	postmitotic	JJ
cord-021063-4y8m33ea	303	32	brain	brain	NN
cord-021063-4y8m33ea	303	33	cells	cell	NNS
cord-021063-4y8m33ea	303	34	Chimerasome	Chimerasome	NNP
cord-021063-4y8m33ea	303	35	-	-	HYPH
cord-021063-4y8m33ea	303	36	mediated	mediate	VBN
cord-021063-4y8m33ea	303	37	gene	gene	NN
cord-021063-4y8m33ea	303	38	transfer	transfer	NN
cord-021063-4y8m33ea	303	39	in	in	IN
cord-021063-4y8m33ea	303	40	vitro	vitro	FW
cord-021063-4y8m33ea	303	41	and	and	CC
cord-021063-4y8m33ea	303	42	in	in	IN
cord-021063-4y8m33ea	303	43	vivo	vivo	NNP
cord-021063-4y8m33ea	303	44	Flow	Flow	NNP
cord-021063-4y8m33ea	303	45	cytofluorometric	cytofluorometric	JJ
cord-021063-4y8m33ea	303	46	investigation	investigation	NN
cord-021063-4y8m33ea	303	47	of	of	IN
cord-021063-4y8m33ea	303	48	the	the	DT
cord-021063-4y8m33ea	303	49	uptake	uptake	NN
cord-021063-4y8m33ea	303	50	by	by	IN
cord-021063-4y8m33ea	303	51	hepatocytes	hepatocyte	NNS
cord-021063-4y8m33ea	303	52	and	and	CC
cord-021063-4y8m33ea	303	53	spleen	spleen	NN
cord-021063-4y8m33ea	303	54	cells	cell	NNS
cord-021063-4y8m33ea	303	55	of	of	IN
cord-021063-4y8m33ea	303	56	targeted	target	VBN
cord-021063-4y8m33ea	303	57	and	and	CC
cord-021063-4y8m33ea	303	58	untargeted	untargeted	JJ
cord-021063-4y8m33ea	303	59	liposomes	liposome	NNS
cord-021063-4y8m33ea	303	60	injected	inject	VBN
cord-021063-4y8m33ea	303	61	intravenously	intravenously	RB
cord-021063-4y8m33ea	303	62	into	into	IN
cord-021063-4y8m33ea	303	63	mice	mouse	NNS
cord-021063-4y8m33ea	303	64	Localization	localization	NN
cord-021063-4y8m33ea	303	65	and	and	CC
cord-021063-4y8m33ea	303	66	induced	induce	VBN
cord-021063-4y8m33ea	303	67	expression	expression	NN
cord-021063-4y8m33ea	303	68	of	of	IN
cord-021063-4y8m33ea	303	69	fusion	fusion	NN
cord-021063-4y8m33ea	303	70	genes	gene	NNS
cord-021063-4y8m33ea	303	71	in	in	IN
cord-021063-4y8m33ea	303	72	the	the	DT
cord-021063-4y8m33ea	303	73	rat	rat	NN
cord-021063-4y8m33ea	303	74	lung	lung	NN
cord-021063-4y8m33ea	303	75	Gene	Gene	NNP
cord-021063-4y8m33ea	303	76	therapy	therapy	NN
cord-021063-4y8m33ea	303	77	for	for	IN
cord-021063-4y8m33ea	303	78	human	human	JJ
cord-021063-4y8m33ea	303	79	inherited	inherit	VBN
cord-021063-4y8m33ea	303	80	disorders	disorder	NNS
cord-021063-4y8m33ea	303	81	:	:	:
cord-021063-4y8m33ea	303	82	techniques	technique	NNS
cord-021063-4y8m33ea	303	83	and	and	CC
cord-021063-4y8m33ea	303	84	status	status	NN
cord-021063-4y8m33ea	303	85	Treatment	treatment	NN
cord-021063-4y8m33ea	303	86	of	of	IN
cord-021063-4y8m33ea	303	87	patients	patient	NNS
cord-021063-4y8m33ea	303	88	with	with	IN
cord-021063-4y8m33ea	303	89	severe	severe	JJ
cord-021063-4y8m33ea	303	90	combined	combine	VBN
cord-021063-4y8m33ea	303	91	immunodeficiency	immunodeficiency	NN
cord-021063-4y8m33ea	303	92	due	due	IN
cord-021063-4y8m33ea	303	93	to	to	IN
cord-021063-4y8m33ea	304	1	adenosine	adenosine	NN
cord-021063-4y8m33ea	304	2	deaminase	deaminase	NNP
cord-021063-4y8m33ea	304	3	(	(	-LRB-
cord-021063-4y8m33ea	304	4	ADA	ADA	NNP
cord-021063-4y8m33ea	304	5	)	)	-RRB-
cord-021063-4y8m33ea	304	6	deficiency	deficiency	NN
cord-021063-4y8m33ea	304	7	by	by	IN
cord-021063-4y8m33ea	304	8	autologous	autologous	JJ
cord-021063-4y8m33ea	304	9	transplantation	transplantation	NN
cord-021063-4y8m33ea	304	10	of	of	IN
cord-021063-4y8m33ea	304	11	genetically	genetically	RB
cord-021063-4y8m33ea	304	12	modified	modify	VBN
cord-021063-4y8m33ea	304	13	bone	bone	NN
cord-021063-4y8m33ea	304	14	marrow	marrow	NN
cord-021063-4y8m33ea	304	15	cells	cell	NNS
cord-021063-4y8m33ea	304	16	Liposome	liposome	NN
cord-021063-4y8m33ea	304	17	-	-	HYPH
cord-021063-4y8m33ea	304	18	mediated	mediate	VBN
cord-021063-4y8m33ea	304	19	transformation	transformation	NN
cord-021063-4y8m33ea	304	20	of	of	IN
cord-021063-4y8m33ea	304	21	eukaryotic	eukaryotic	JJ
cord-021063-4y8m33ea	304	22	cells	cell	NNS
cord-021063-4y8m33ea	304	23	Synthetic	synthetic	JJ
cord-021063-4y8m33ea	304	24	cationic	cationic	JJ
cord-021063-4y8m33ea	304	25	amphiphiles	amphiphile	NNS
cord-021063-4y8m33ea	304	26	for	for	IN
cord-021063-4y8m33ea	304	27	liposome	liposome	NN
cord-021063-4y8m33ea	304	28	-	-	HYPH
cord-021063-4y8m33ea	304	29	mediated	mediate	VBN
cord-021063-4y8m33ea	304	30	DNA	dna	NN
cord-021063-4y8m33ea	304	31	transfection	transfection	NN
cord-021063-4y8m33ea	304	32	Basic	basic	JJ
cord-021063-4y8m33ea	304	33	protein	protein	NN
cord-021063-4y8m33ea	304	34	enhances	enhance	VBZ
cord-021063-4y8m33ea	304	35	the	the	DT
cord-021063-4y8m33ea	304	36	incorporation	incorporation	NN
cord-021063-4y8m33ea	304	37	of	of	IN
cord-021063-4y8m33ea	304	38	DNA	DNA	NNP
cord-021063-4y8m33ea	304	39	into	into	IN
cord-021063-4y8m33ea	304	40	lipid	lipid	NN
cord-021063-4y8m33ea	304	41	vesicles	vesicle	NNS
cord-021063-4y8m33ea	304	42	:	:	:
cord-021063-4y8m33ea	304	43	model	model	NN
cord-021063-4y8m33ea	304	44	for	for	IN
cord-021063-4y8m33ea	304	45	the	the	DT
cord-021063-4y8m33ea	304	46	formation	formation	NN
cord-021063-4y8m33ea	304	47	of	of	IN
cord-021063-4y8m33ea	304	48	primordial	primordial	JJ
cord-021063-4y8m33ea	304	49	cells	cell	NNS
cord-021063-4y8m33ea	304	50	Experimental	experimental	JJ
cord-021063-4y8m33ea	304	51	models	model	NNS
cord-021063-4y8m33ea	304	52	of	of	IN
cord-021063-4y8m33ea	304	53	altering	alter	VBG
cord-021063-4y8m33ea	304	54	the	the	DT
cord-021063-4y8m33ea	304	55	blood	blood	NN
cord-021063-4y8m33ea	304	56	-	-	HYPH
cord-021063-4y8m33ea	304	57	brain	brain	NN
cord-021063-4y8m33ea	304	58	barrier	barrier	NN
cord-021063-4y8m33ea	304	59	Direct	direct	JJ
cord-021063-4y8m33ea	304	60	injection	injection	NN
cord-021063-4y8m33ea	304	61	of	of	IN
cord-021063-4y8m33ea	304	62	hepatitis	hepatitis	NN
cord-021063-4y8m33ea	304	63	B	B	NNP
cord-021063-4y8m33ea	304	64	virus	virus	NN
cord-021063-4y8m33ea	304	65	DNA	dna	NN
cord-021063-4y8m33ea	304	66	into	into	IN
cord-021063-4y8m33ea	304	67	liver	liver	NN
cord-021063-4y8m33ea	304	68	induced	induce	VBN
cord-021063-4y8m33ea	304	69	hepatitis	hepatitis	NN
cord-021063-4y8m33ea	304	70	in	in	IN
cord-021063-4y8m33ea	304	71	adult	adult	NN
cord-021063-4y8m33ea	304	72	rats	rat	NNS
cord-021063-4y8m33ea	304	73	Activity	Activity	NNP
cord-021063-4y8m33ea	304	74	of	of	IN
cord-021063-4y8m33ea	304	75	amphipathic	amphipathic	NNP
cord-021063-4y8m33ea	304	76	poly(ethylene	poly(ethylene	NNP
cord-021063-4y8m33ea	304	77	glycol	glycol	NN
cord-021063-4y8m33ea	304	78	)	)	-RRB-
cord-021063-4y8m33ea	304	79	5000	5000	CD
cord-021063-4y8m33ea	304	80	to	to	TO
cord-021063-4y8m33ea	304	81	prolong	prolong	VB
cord-021063-4y8m33ea	304	82	the	the	DT
cord-021063-4y8m33ea	304	83	circulation	circulation	NN
cord-021063-4y8m33ea	304	84	time	time	NN
cord-021063-4y8m33ea	304	85	of	of	IN
cord-021063-4y8m33ea	304	86	liposomes	liposome	NNS
cord-021063-4y8m33ea	304	87	depends	depend	VBZ
cord-021063-4y8m33ea	304	88	on	on	IN
cord-021063-4y8m33ea	304	89	the	the	DT
cord-021063-4y8m33ea	304	90	liposome	liposome	NN
cord-021063-4y8m33ea	304	91	size	size	NN
cord-021063-4y8m33ea	304	92	and	and	CC
cord-021063-4y8m33ea	304	93	is	be	VBZ
cord-021063-4y8m33ea	304	94	unfavorable	unfavorable	JJ
cord-021063-4y8m33ea	304	95	for	for	IN
cord-021063-4y8m33ea	304	96	immunoliposome	immunoliposome	NN
cord-021063-4y8m33ea	304	97	binding	bind	VBG
cord-021063-4y8m33ea	304	98	to	to	TO
cord-021063-4y8m33ea	304	99	target	target	VB
cord-021063-4y8m33ea	304	100	Gene	Gene	NNP
cord-021063-4y8m33ea	304	101	therapy	therapy	NN
cord-021063-4y8m33ea	304	102	:	:	:
cord-021063-4y8m33ea	304	103	Adenovirus	Adenovirus	NNP
cord-021063-4y8m33ea	304	104	vectors	vector	NNS
cord-021063-4y8m33ea	304	105	Fusion	fusion	NN
cord-021063-4y8m33ea	304	106	-	-	HYPH
cord-021063-4y8m33ea	304	107	mediated	mediate	VBN
cord-021063-4y8m33ea	304	108	microinjection	microinjection	NN
cord-021063-4y8m33ea	304	109	of	of	IN
cord-021063-4y8m33ea	304	110	liposome	liposome	NN
cord-021063-4y8m33ea	304	111	-	-	HYPH
cord-021063-4y8m33ea	304	112	enclosed	enclose	VBN
cord-021063-4y8m33ea	304	113	DNA	dna	NN
cord-021063-4y8m33ea	304	114	into	into	IN
cord-021063-4y8m33ea	304	115	cultured	cultured	JJ
cord-021063-4y8m33ea	304	116	cells	cell	NNS
cord-021063-4y8m33ea	304	117	with	with	IN
cord-021063-4y8m33ea	304	118	the	the	DT
cord-021063-4y8m33ea	304	119	aid	aid	NN
cord-021063-4y8m33ea	304	120	of	of	IN
cord-021063-4y8m33ea	304	121	influenza	influenza	NN
cord-021063-4y8m33ea	304	122	virus	virus	NN
cord-021063-4y8m33ea	304	123	glycoproteins	glycoprotein	NNS
cord-021063-4y8m33ea	304	124	Sterically	sterically	RB
cord-021063-4y8m33ea	304	125	stabilized	stabilize	VBD
cord-021063-4y8m33ea	304	126	liposomes	liposome	NNS
cord-021063-4y8m33ea	304	127	:	:	:
cord-021063-4y8m33ea	305	1	A	a	DT
cord-021063-4y8m33ea	305	2	hypothesis	hypothesis	NN
cord-021063-4y8m33ea	305	3	on	on	IN
cord-021063-4y8m33ea	305	4	the	the	DT
cord-021063-4y8m33ea	305	5	molecular	molecular	JJ
cord-021063-4y8m33ea	305	6	origin	origin	NN
cord-021063-4y8m33ea	305	7	of	of	IN
cord-021063-4y8m33ea	305	8	the	the	DT
cord-021063-4y8m33ea	305	9	extended	extended	JJ
cord-021063-4y8m33ea	305	10	circulation	circulation	NN
cord-021063-4y8m33ea	305	11	times	time	NNS
cord-021063-4y8m33ea	305	12	Recognition	Recognition	NNP
cord-021063-4y8m33ea	305	13	of	of	IN
cord-021063-4y8m33ea	305	14	liposomes	liposome	NNS
cord-021063-4y8m33ea	305	15	by	by	IN
cord-021063-4y8m33ea	305	16	cells	cell	NNS
cord-021063-4y8m33ea	305	17	:	:	:
cord-021063-4y8m33ea	305	18	In	in	IN
cord-021063-4y8m33ea	305	19	vitro	vitro	FW
cord-021063-4y8m33ea	305	20	binding	binding	NN
cord-021063-4y8m33ea	305	21	and	and	CC
cord-021063-4y8m33ea	305	22	endocytosis	endocytosis	NN
cord-021063-4y8m33ea	305	23	mediated	mediate	VBN
cord-021063-4y8m33ea	305	24	by	by	IN
cord-021063-4y8m33ea	305	25	specific	specific	JJ
cord-021063-4y8m33ea	305	26	lipid	lipid	NN
cord-021063-4y8m33ea	305	27	headgroups	headgroups	NNP
cord-021063-4y8m33ea	305	28	and	and	CC
cord-021063-4y8m33ea	305	29	surface	surface	NN
cord-021063-4y8m33ea	305	30	charge	charge	NN
cord-021063-4y8m33ea	305	31	density	density	NN
cord-021063-4y8m33ea	305	32	Interactions	interaction	NNS
cord-021063-4y8m33ea	305	33	of	of	IN
cord-021063-4y8m33ea	305	34	mammalian	mammalian	JJ
cord-021063-4y8m33ea	305	35	cells	cell	NNS
cord-021063-4y8m33ea	305	36	with	with	IN
cord-021063-4y8m33ea	305	37	lipid	lipid	NN
cord-021063-4y8m33ea	305	38	dispersions	dispersion	NNS
cord-021063-4y8m33ea	305	39	containing	contain	VBG
cord-021063-4y8m33ea	305	40	novel	novel	NN
cord-021063-4y8m33ea	305	41	metabolizable	metabolizable	JJ
cord-021063-4y8m33ea	305	42	cationic	cationic	JJ
cord-021063-4y8m33ea	305	43	amphiphiles	amphiphile	NNS
cord-021063-4y8m33ea	305	44	.	.	.
cord-021063-4y8m33ea	306	1	B	B	NNP
cord-021063-4y8m33ea	306	2	iochim	iochim	NNP
cord-021063-4y8m33ea	306	3	.	.	.
cord-021063-4y8m33ea	307	1	B	B	NNP
cord-021063-4y8m33ea	307	2	iophys	iophys	NNP
cord-021063-4y8m33ea	307	3	pH	ph	NN
cord-021063-4y8m33ea	307	4	-	-	HYPH
cord-021063-4y8m33ea	307	5	sensitive	sensitive	JJ
cord-021063-4y8m33ea	307	6	,	,	,
cord-021063-4y8m33ea	307	7	plasma	plasma	NN
cord-021063-4y8m33ea	307	8	-	-	HYPH
cord-021063-4y8m33ea	307	9	stable	stable	JJ
cord-021063-4y8m33ea	307	10	liposomes	liposome	NNS
cord-021063-4y8m33ea	307	11	with	with	IN
cord-021063-4y8m33ea	307	12	relatively	relatively	RB
cord-021063-4y8m33ea	307	13	prolonged	prolong	VBN
cord-021063-4y8m33ea	307	14	residence	residence	NN
cord-021063-4y8m33ea	307	15	in	in	IN
cord-021063-4y8m33ea	307	16	circulation	circulation	NN
cord-021063-4y8m33ea	308	1	Optimized	optimized	JJ
cord-021063-4y8m33ea	308	2	procedures	procedure	NNS
cord-021063-4y8m33ea	308	3	for	for	IN
cord-021063-4y8m33ea	308	4	the	the	DT
cord-021063-4y8m33ea	308	5	coupling	coupling	NN
cord-021063-4y8m33ea	308	6	of	of	IN
cord-021063-4y8m33ea	308	7	proteins	protein	NNS
cord-021063-4y8m33ea	308	8	to	to	IN
cord-021063-4y8m33ea	308	9	liposomes	liposome	VBN
cord-021063-4y8m33ea	308	10	Vesicles	Vesicles	NNP
cord-021063-4y8m33ea	308	11	of	of	IN
cord-021063-4y8m33ea	308	12	variable	variable	JJ
cord-021063-4y8m33ea	308	13	sizes	size	NNS
cord-021063-4y8m33ea	308	14	produced	produce	VBN
cord-021063-4y8m33ea	308	15	by	by	IN
cord-021063-4y8m33ea	308	16	a	a	DT
cord-021063-4y8m33ea	308	17	rapid	rapid	JJ
cord-021063-4y8m33ea	308	18	extrusion	extrusion	NN
cord-021063-4y8m33ea	308	19	procedure	procedure	NN
cord-021063-4y8m33ea	308	20	Karyophilic	Karyophilic	NNP
cord-021063-4y8m33ea	308	21	properties	property	NNS
cord-021063-4y8m33ea	308	22	of	of	IN
cord-021063-4y8m33ea	308	23	Semliki	Semliki	NNP
cord-021063-4y8m33ea	308	24	Forest	Forest	NNP
cord-021063-4y8m33ea	308	25	virus	virus	NN
cord-021063-4y8m33ea	308	26	nucleocapsid	nucleocapsid	NN
cord-021063-4y8m33ea	308	27	protein	protein	NNP
cord-021063-4y8m33ea	308	28	Human	human	JJ
cord-021063-4y8m33ea	308	29	gene	gene	NN
cord-021063-4y8m33ea	308	30	therapy	therapy	NN
cord-021063-4y8m33ea	308	31	Direct	direct	JJ
cord-021063-4y8m33ea	308	32	gene	gene	NN
cord-021063-4y8m33ea	308	33	transfer	transfer	NN
cord-021063-4y8m33ea	308	34	with	with	IN
cord-021063-4y8m33ea	308	35	DNA	dna	NN
cord-021063-4y8m33ea	308	36	-	-	HYPH
cord-021063-4y8m33ea	308	37	liposome	liposome	NN
cord-021063-4y8m33ea	308	38	complexes	complex	NNS
cord-021063-4y8m33ea	308	39	in	in	IN
cord-021063-4y8m33ea	308	40	melanoma	melanoma	NN
cord-021063-4y8m33ea	308	41	:	:	:
cord-021063-4y8m33ea	308	42	expression	expression	NN
cord-021063-4y8m33ea	308	43	,	,	,
cord-021063-4y8m33ea	308	44	biologic	biologic	JJ
cord-021063-4y8m33ea	308	45	activity	activity	NN
cord-021063-4y8m33ea	308	46	,	,	,
cord-021063-4y8m33ea	308	47	and	and	CC
cord-021063-4y8m33ea	308	48	lack	lack	NN
cord-021063-4y8m33ea	308	49	of	of	IN
cord-021063-4y8m33ea	308	50	toxicity	toxicity	NN
cord-021063-4y8m33ea	308	51	in	in	IN
cord-021063-4y8m33ea	308	52	humans	human	NNS
cord-021063-4y8m33ea	309	1	The	the	DT
cord-021063-4y8m33ea	309	2	improved	improved	JJ
cord-021063-4y8m33ea	309	3	efficient	efficient	JJ
cord-021063-4y8m33ea	309	4	method	method	NN
cord-021063-4y8m33ea	309	5	for	for	IN
cord-021063-4y8m33ea	309	6	introducing	introduce	VBG
cord-021063-4y8m33ea	309	7	macromolecules	macromolecule	NNS
cord-021063-4y8m33ea	309	8	into	into	IN
cord-021063-4y8m33ea	309	9	cells	cell	NNS
cord-021063-4y8m33ea	309	10	using	use	VBG
cord-021063-4y8m33ea	309	11	HVJ	HVJ	NNP
cord-021063-4y8m33ea	309	12	(	(	-LRB-
cord-021063-4y8m33ea	309	13	Sendai	Sendai	NNP
cord-021063-4y8m33ea	309	14	virus	virus	NN
cord-021063-4y8m33ea	309	15	)	)	-RRB-
cord-021063-4y8m33ea	309	16	liposomes	liposome	VBN
cord-021063-4y8m33ea	309	17	with	with	IN
cord-021063-4y8m33ea	309	18	gangliosides	ganglioside	NNS
cord-021063-4y8m33ea	309	19	Fusion	Fusion	NNP
cord-021063-4y8m33ea	309	20	of	of	IN
cord-021063-4y8m33ea	309	21	influenza	influenza	NN
cord-021063-4y8m33ea	309	22	virus	virus	NN
cord-021063-4y8m33ea	309	23	particles	particle	NNS
cord-021063-4y8m33ea	309	24	with	with	IN
cord-021063-4y8m33ea	309	25	liposomes	liposome	NNS
cord-021063-4y8m33ea	309	26	:	:	:
cord-021063-4y8m33ea	310	1	Requirement	requirement	NN
cord-021063-4y8m33ea	310	2	for	for	IN
cord-021063-4y8m33ea	310	3	cholesterol	cholesterol	NN
cord-021063-4y8m33ea	310	4	and	and	CC
cord-021063-4y8m33ea	310	5	virus	virus	NN
cord-021063-4y8m33ea	310	6	receptors	receptor	NNS
cord-021063-4y8m33ea	310	7	to	to	TO
cord-021063-4y8m33ea	310	8	allow	allow	VB
cord-021063-4y8m33ea	310	9	fusion	fusion	NN
cord-021063-4y8m33ea	310	10	with	with	IN
cord-021063-4y8m33ea	310	11	and	and	CC
cord-021063-4y8m33ea	310	12	lysis	lysis	NN
cord-021063-4y8m33ea	310	13	of	of	IN
cord-021063-4y8m33ea	310	14	neutral	neutral	JJ
cord-021063-4y8m33ea	310	15	but	but	CC
cord-021063-4y8m33ea	310	16	not	not	RB
cord-021063-4y8m33ea	310	17	negatively	negatively	RB
cord-021063-4y8m33ea	310	18	charged	charge	VBN
cord-021063-4y8m33ea	310	19	liposomes	liposome	NNS
cord-021063-4y8m33ea	310	20	Gene	Gene	NNP
cord-021063-4y8m33ea	310	21	therapy	therapy	NN
cord-021063-4y8m33ea	310	22	for	for	IN
cord-021063-4y8m33ea	310	23	the	the	DT
cord-021063-4y8m33ea	310	24	treatment	treatment	NN
cord-021063-4y8m33ea	310	25	of	of	IN
cord-021063-4y8m33ea	310	26	brain	brain	NN
cord-021063-4y8m33ea	310	27	tumors	tumor	NNS
cord-021063-4y8m33ea	310	28	using	use	VBG
cord-021063-4y8m33ea	310	29	intra	intra	JJ
cord-021063-4y8m33ea	310	30	-	-	JJ
cord-021063-4y8m33ea	310	31	tumoral	tumoral	JJ
cord-021063-4y8m33ea	310	32	transduction	transduction	NN
cord-021063-4y8m33ea	310	33	with	with	IN
cord-021063-4y8m33ea	310	34	the	the	DT
cord-021063-4y8m33ea	310	35	thymidine	thymidine	NN
cord-021063-4y8m33ea	310	36	kinase	kinase	NN
cord-021063-4y8m33ea	310	37	gene	gene	NN
cord-021063-4y8m33ea	310	38	and	and	CC
cord-021063-4y8m33ea	310	39	intravenous	intravenous	JJ
cord-021063-4y8m33ea	310	40	ganciclovir	ganciclovir	NN
cord-021063-4y8m33ea	310	41	Heterologous	Heterologous	NNP
cord-021063-4y8m33ea	310	42	introns	intron	NNS
cord-021063-4y8m33ea	310	43	can	can	MD
cord-021063-4y8m33ea	310	44	enhance	enhance	VB
cord-021063-4y8m33ea	310	45	expression	expression	NN
cord-021063-4y8m33ea	310	46	oftransgenes	oftransgene	NNS
cord-021063-4y8m33ea	310	47	in	in	IN
cord-021063-4y8m33ea	310	48	mice	mouse	NNS
cord-021063-4y8m33ea	310	49	Cochleate	Cochleate	NNP
cord-021063-4y8m33ea	310	50	lipid	lipid	NN
cord-021063-4y8m33ea	310	51	cylinders	cylinder	NNS
cord-021063-4y8m33ea	310	52	:	:	:
cord-021063-4y8m33ea	310	53	formation	formation	NN
cord-021063-4y8m33ea	310	54	by	by	IN
cord-021063-4y8m33ea	310	55	fusion	fusion	NN
cord-021063-4y8m33ea	310	56	of	of	IN
cord-021063-4y8m33ea	310	57	unilamellar	unilamellar	JJ
cord-021063-4y8m33ea	310	58	lipid	lipid	NN
cord-021063-4y8m33ea	310	59	vesicles	vesicle	NNS
cord-021063-4y8m33ea	311	1	Targeted	target	VBN
cord-021063-4y8m33ea	311	2	integration	integration	NN
cord-021063-4y8m33ea	311	3	of	of	IN
cord-021063-4y8m33ea	311	4	adeno	adeno	NN
cord-021063-4y8m33ea	311	5	-	-	HYPH
cord-021063-4y8m33ea	311	6	associated	associate	VBN
cord-021063-4y8m33ea	311	7	virus	virus	NN
cord-021063-4y8m33ea	311	8	(	(	-LRB-
cord-021063-4y8m33ea	311	9	AAV	AAV	NNP
cord-021063-4y8m33ea	311	10	)	)	-RRB-
cord-021063-4y8m33ea	311	11	into	into	IN
cord-021063-4y8m33ea	311	12	human	human	JJ
cord-021063-4y8m33ea	311	13	chromosome	chromosome	NN
cord-021063-4y8m33ea	311	14	19	19	CD
cord-021063-4y8m33ea	311	15	Endocytosis	Endocytosis	NNP
cord-021063-4y8m33ea	311	16	and	and	CC
cord-021063-4y8m33ea	311	17	intracellular	intracellular	JJ
cord-021063-4y8m33ea	311	18	fate	fate	NN
cord-021063-4y8m33ea	311	19	of	of	IN
cord-021063-4y8m33ea	311	20	liposomes	liposome	NNS
cord-021063-4y8m33ea	311	21	using	use	VBG
cord-021063-4y8m33ea	311	22	pyranine	pyranine	NNP
cord-021063-4y8m33ea	311	23	as	as	IN
cord-021063-4y8m33ea	311	24	probe	probe	NN
cord-021063-4y8m33ea	311	25	Aerosol	Aerosol	NNP
cord-021063-4y8m33ea	311	26	gene	gene	NN
cord-021063-4y8m33ea	311	27	delivery	delivery	NN
cord-021063-4y8m33ea	311	28	in	in	IN
cord-021063-4y8m33ea	311	29	vivo	vivo	NN
cord-021063-4y8m33ea	311	30	Entrapment	entrapment	NN
cord-021063-4y8m33ea	311	31	of	of	IN
cord-021063-4y8m33ea	311	32	high	high	JJ
cord-021063-4y8m33ea	311	33	-	-	HYPH
cord-021063-4y8m33ea	311	34	molecular	molecular	JJ
cord-021063-4y8m33ea	311	35	-	-	HYPH
cord-021063-4y8m33ea	311	36	mass	mass	NN
cord-021063-4y8m33ea	311	37	DNA	dna	NN
cord-021063-4y8m33ea	311	38	molecules	molecule	NNS
cord-021063-4y8m33ea	311	39	in	in	IN
cord-021063-4y8m33ea	311	40	liposomes	liposome	NNS
cord-021063-4y8m33ea	311	41	for	for	IN
cord-021063-4y8m33ea	311	42	the	the	DT
cord-021063-4y8m33ea	311	43	genetic	genetic	JJ
cord-021063-4y8m33ea	311	44	transformation	transformation	NN
cord-021063-4y8m33ea	311	45	of	of	IN
cord-021063-4y8m33ea	311	46	animal	animal	NN
cord-021063-4y8m33ea	311	47	cells	cell	NNS
cord-021063-4y8m33ea	311	48	Transfer	transfer	NN
cord-021063-4y8m33ea	311	49	of	of	IN
cord-021063-4y8m33ea	311	50	condensed	condense	VBN
cord-021063-4y8m33ea	311	51	viral	viral	JJ
cord-021063-4y8m33ea	311	52	DNA	dna	NN
cord-021063-4y8m33ea	311	53	into	into	IN
cord-021063-4y8m33ea	311	54	eukaryotic	eukaryotic	JJ
cord-021063-4y8m33ea	311	55	cells	cell	NNS
cord-021063-4y8m33ea	311	56	using	use	VBG
cord-021063-4y8m33ea	311	57	proteoliposomes	proteoliposome	NNS
cord-021063-4y8m33ea	311	58	Direct	direct	JJ
cord-021063-4y8m33ea	311	59	in	in	IN
cord-021063-4y8m33ea	311	60	vivo	vivo	NN
cord-021063-4y8m33ea	311	61	gene	gene	NN
cord-021063-4y8m33ea	311	62	introduction	introduction	NN
cord-021063-4y8m33ea	311	63	into	into	IN
cord-021063-4y8m33ea	311	64	rat	rat	NN
cord-021063-4y8m33ea	311	65	kidney	kidney	NN
cord-021063-4y8m33ea	311	66	Membrane	membrane	NN
cord-021063-4y8m33ea	311	67	fusion	fusion	NN
cord-021063-4y8m33ea	311	68	proteins	protein	NNS
cord-021063-4y8m33ea	311	69	of	of	IN
cord-021063-4y8m33ea	311	70	enveloped	envelop	VBN
cord-021063-4y8m33ea	311	71	animal	animal	NN
cord-021063-4y8m33ea	311	72	viruses	virus	NNS